@17:45:11.88UTC03-Oct-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @17:45:14.46 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @17:45:18.44 Polling Can every 10:00 @17:45:18.45 Cleanly exited at 17:35:12UTC03-Oct-22 @17:45:18.49 Loading /home/esp/esp2/mission/DA_WCR_10322.rb mission @17:45:18.54 GoodNews.email "Starting DA_WCR_10322 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@17:45:18, 22.3C, 18% humidity, 15.6psia, 13.836V, 0.257A, 0.257A avg, 3.56W",Subject: "DA_WCR_10322 begun" @17:45:18.56
Preparing to sleep until + 00:00:05 @17:45:18.62 Asleep til + 00:00:05 @17:45:18.63 Continuing at 17:45 @17:45:18.92 Gate.power :core,:OFF @17:45:18.93 Gate.power :raw,:OFF @17:45:18.94 Shutdown @17:45:22.49 GoodNews.email "Then, in tube 2: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_10322.rb:14\n{1 => 0,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:45:18, 22.3C, 18% humidity, 15.6psia, 13.836V, 0.257A, 0.257A avg, 3.56W",Subject: "Asleep until 17:45" @17:45:23.54
Powering core bus @17:45:28.90 Gate.power :raw,:ON @17:45:30.37 CS.configure :default @17:45:30.55 PS.configure :default @17:45:30.73 SS.configure :default @17:45:30.94 CC.configure :default @17:45:31.11 PC.configure :default @17:45:31.32 Forearm.configure :loose @17:45:31.50 Elbow.configure :loose @17:45:31.77 SC.configure :loose @17:45:31.95 SE.configure :default @17:45:32.03 CH.coast @17:45:32.14 CH.configure :default @17:45:32.20 PH.coast @17:45:32.33 PH.configure :default @17:45:32.40 All dwarf controllers running firmware version 3.85 @17:45:32.55 Valve.reset @17:45:33.05 Intake.select :close @17:45:33.09 PSV.dial :air @17:45:33.18 Exhaust.select :close @17:45:33.23 CTV.dial :air @17:45:33.45 CSV.dial :waste1 @17:45:33.52 PS.reconfigure :default @17:45:33.80 PS.home.seek 0ml @17:45:33.84
Forearm.reconfigure :loose @17:45:33.99 Forearm.home.seek :retract @17:45:34.13 SSV.dial :spit,avoiding: :exhaust @17:45:34.36 CS.reconfigure :fast @17:45:34.43 SS.reconfigure :default @17:45:34.66 SS.home.seek 0ml @17:45:34.74 CS.home.seek 0ml @17:45:34.93 Hand.close @17:45:34.99
Elbow.jog 1500 @17:45:35.48 Elbow.reconfigure :loose @17:45:35.57 Elbow.home.seek :clear @17:45:38.94 SE.reconfigure :default @17:45:39.04 SE.home.seek :down @17:45:39.80 SC.reconfigure :tight @17:45:39.91 SC.home.seek :initial @17:45:42.58 PS.stop @17:46:01.22 upsync @17:46:10.00 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @17:46:10.01
Retry #1 of 3 @17:46:10.07 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @17:46:11.26 SC.configure :limp @17:46:11.31 Forearm.seek :retract @17:46:11.37 Elbow.seek :hammer @17:46:13.39 Forearm.reconfigure :tight @17:46:13.43 Forearm.seek retracted @17:46:13.77 PC.reconfigure :default @17:46:13.79
CC.reconfigure :default @17:46:13.87 PC.home.seek :open @17:46:13.90
CC.home.seek :open @17:46:17.09 Phase [initialPurge; da [100, 100] #da and wsr are run simultanelously] @17:46:17.10 Notice.email "At 17:46:17 began in tube 2: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_10322.rb:14\n{1 => 0,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:46:17, 22.3C, 18% humidity, 15.6psia, 13.787V, 0.416A, 0.416A avg, 5.74W",Subject: "Started DA_WCR_10322 phase initialPurge; da [100, 100] #da and wsr are run simultanelously" @17:46:17.12
Starting initialPurge @17:46:17.15 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:46:17.22 Hand.open @17:46:18.11 Elbow.reconfigure :tight @17:46:18.15 Elbow.seek FlushPuck::Garage @17:46:20.05 Elbow.reconfigure :loose @17:46:20.14 Forearm.reconfigure :loose @17:46:20.18 Forearm.seek :garage @17:46:21.30 Hand.close @17:46:22.17 Forearm.seek :retract @17:46:23.35 Elbow.reconfigure :tight @17:46:23.43 Elbow.seek CC @17:46:26.51 Elbow.reconfigure :loose @17:46:26.70 Forearm.reconfigure :tight @17:46:26.78 Forearm.seek CC @17:46:28.37 CC.seek :closed @17:46:32.70 Collection Clamp onSpring/offSpring for 8/25 samples, on/off torque 1.91 @17:46:32.94 Hand.open @17:46:33.84 Forearm.reconfigure :loose @17:46:33.94 Forearm.seek :retract @17:46:35.03 Flushing the Collection Valve::Manifold @17:46:35.25 CSV.dial CSR @17:46:36.66 Pulling 4.999ml of CSR.flush @17:46:36.68 CSR.select :flush @17:46:36.87 CS.reconfigure :default @17:46:36.95 CS.seek 5ml @17:46:50.46 delay 5 seconds @17:46:55.48 CSR.select :air @17:46:55.72 CS.reconfigure :fast @17:46:55.81 CS.seek 7.5ml @17:46:58.97 upsync @17:47:02.19
CSV.dial :waste @17:47:03.46 CS.reconfigure :default @17:47:03.50 CS.seek 0ml @17:47:23.19 Purging external Intake @17:47:23.30 CSV.dial :flush @17:47:23.76 CS.reconfigure :fast @17:47:23.80 CS.seek 10ml @17:47:46.94 delay 5 seconds @17:47:51.96 CSV.dial CBV @17:47:51.99
CBV.dial CSV @17:47:52.77 CTV.dial :intake @17:47:53.50 Intake.select :open @17:47:53.61 CS.seek 0ml @17:48:16.37 Intake.select :close @17:48:16.47 CTV.dial :air @17:48:17.22 Flushing upper manifold @17:48:17.33 CSV.dial :flush @17:48:18.07 CS.seek 5ml @17:48:30.06 delay 5 seconds @17:48:35.08 CSV.dial :air @17:48:35.57 CS.seek 7.001ml @17:48:40.88 CSV.dial CBV @17:48:40.91
CBV.dial CSV @17:48:41.91 CTV.dial :air @17:48:41.92 CS.seek 0ml @17:48:58.19 delay 7 seconds @17:49:05.21 CS.seek 3ml @17:49:12.75 CSV.dial :waste @17:49:13.19 CS.seek 0ml @17:49:20.85 CSV.dial :flush @17:49:21.29 CS.seek 3ml @17:49:28.81 delay 5 seconds @17:49:33.83 CSV.dial :air @17:49:34.34 CS.seek 5ml @17:49:39.59 CSV.dial :air @17:49:39.61 CS.seek 0ml @17:49:51.43 delay 7 seconds @17:49:58.46 CS.seek 3ml @17:50:06.06 CSV.dial :waste @17:50:06.78 CS.seek 0ml @17:50:14.18 Garaging FlushPuck in Collection Clamp @17:50:14.25 Elbow.reconfigure :tight @17:50:14.29 Elbow.seek CC @17:50:14.40 Elbow.reconfigure :loose @17:50:14.49 Forearm.seek CC @17:50:15.43 Hand.close @17:50:16.12 CC.seek :open @17:50:20.45 Forearm.seek :retract @17:50:21.56 Elbow.reconfigure :tight @17:50:21.60 Elbow.seek :garage @17:50:24.71 Elbow.reconfigure :loose @17:50:24.80 Forearm.reconfigure :tight @17:50:24.84 Forearm.seek :garage @17:50:25.93 Hand.open @17:50:27.11 Forearm.reconfigure :loose @17:50:27.15 Forearm.seek :retract @17:50:28.20 da sampling at most 100ml, wcr at most 100ml @17:50:28.22 DAfans.email "Started da in tube 2 at 17:50:28UTC03-Oct-22\n{1 => 0,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:46:17, 22.3C, 18% humidity, 15.6psia, 13.787V, 0.416A, 0.416A avg, 5.74W",Subject: "da sampling at most 100ml, wcr at most 100ml" @17:50:28.26
Loading sh1 puck from tube 2 into Collection Clamp @17:50:28.49 SC.reconfigure :tight @17:50:28.55 SC.seek 2 @17:50:41.55 SC.configure :limp @17:50:41.98 Elbow.reconfigure :tight @17:50:42.06 Elbow.seek 2 @17:50:44.14 Forearm.reconfigure :tight @17:50:44.24 Forearm.seek 2 + 700 counts @17:50:45.43 SE.reconfigure :pick @17:50:45.53 SE.seek :beamBreak @17:50:46.51 SE.configure :default @17:50:46.80 Elbow.seek 2 @17:50:47.13 Forearm.seek 2 + 300 counts @17:50:47.55 Forearm.reconfigure :loose @17:50:47.62 Hand.close @17:50:48.42 SE.seek :down @17:50:48.82
Forearm.seek :retract @17:50:49.14 SE.stop @17:50:49.84
Elbow.seek CC @17:50:51.44 Elbow.reconfigure :loose @17:50:51.63 Forearm.reconfigure :tight @17:50:51.71 Forearm.seek CC @17:50:53.39 CC.seek :closed @17:50:57.72 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.76 @17:50:57.74 Hand.open @17:50:58.63 Forearm.reconfigure :loose @17:50:58.71 Forearm.seek :retract @17:50:59.76 collectSample 100ml w/bubble point of 24psi @17:50:59.78 Priming 7ml @17:50:59.80 Intake.select :close @17:50:59.82 Exhaust.select :close @17:50:59.84 SS.hold @17:51:00.21 Calibrating pressure and temperature sensors @17:51:00.28 CTV.dial :air @17:51:00.31 SSV.dial :spit,avoiding: :exhaust @17:51:00.34 SS.reconfigure :fast @17:51:00.41 SS.seek 5ml @17:51:03.32 SSV.dial CBV @17:51:03.36
CBV.dial SSV @17:51:04.75 delay 3.8 seconds @17:51:07.21 upsync @17:51:08.66
SSV.dial :spit @17:51:09.98 SS.seek 0ml @17:51:12.50 SSV.dial CBV @17:51:13.79 CTV.dial :intake @17:51:14.55 SSV.dial CBV @17:51:14.57
CBV.dial SSV @17:51:14.58 Allowing 40s for 7.0ml of remaining 7.0ml @17:51:14.59 Intake.select :open @17:51:16.74 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:51:16.76 SS.reconfigure :inhaling 2.14ml/s @17:51:16.80 SS.seek 7ml @17:51:23.78 Stroke completed -- waiting for pressure to equalize ... @17:51:23.79 SS.reconfigure :exhale @17:51:23.83 SS.hold @17:51:24.13 Intake.select :close @17:51:24.27 SSV.dial :exhaust @17:51:25.00 Exhaust.select :open @17:51:25.11 SS.seek 0ml @17:51:28.93 Exhaust.select :close @17:51:29.03 Sampling 100.0ml @17:51:29.04 Intake.select :close @17:51:29.05 Exhaust.select :close @17:51:29.06 SS.reconfigure :default @17:51:29.10 SS.hold @17:51:29.28 CTV.dial :intake @17:51:29.29 SSV.dial CBV @17:51:29.32
CBV.dial SSV @17:51:30.07 Allowing 130s for 25.0ml of remaining 100.0ml @17:51:30.08 Intake.select :open @17:51:32.23 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:51:32.24 SS.reconfigure :inhaling 2.14ml/s @17:51:32.29 SS.seek 25ml @17:51:42.45 Vacuum too high -- waiting up to 40 seconds @17:51:42.46 SS.reconfigure :exhale @17:51:42.50 SS.hold @17:51:43.19 Intake @10.0psia, vacuum from 4.5 to 7.5psi @17:51:43.20 SS.reconfigure :inhaling 1.93ml/s @17:51:43.24 SS.seek 25ml @17:51:49.77 Stroke completed -- waiting for pressure to equalize ... @17:51:49.78 SS.reconfigure :exhale @17:51:49.82 SS.hold @17:51:50.38 Intake.select :close @17:51:50.52 SSV.dial :exhaust @17:51:51.27 Exhaust.select :open @17:51:51.79 SS.seek 0ml @17:52:00.55 Exhaust.select :close @17:52:00.65 SSV.dial CBV @17:52:00.67
CBV.dial SSV @17:52:01.43 Allowing 130s for 25.0ml of remaining 75.0ml @17:52:01.44 Intake.select :open @17:52:03.59 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:52:03.61 SS.reconfigure :inhaling 1.93ml/s @17:52:03.65 SS.seek 25ml @17:52:19.66 Stroke completed -- waiting for pressure to equalize ... @17:52:19.67 SS.reconfigure :exhale @17:52:19.71 SS.hold @17:52:20.65 Intake.select :close @17:52:20.79 SSV.dial :exhaust @17:52:21.52 Exhaust.select :open @17:52:21.62 SS.seek 0ml @17:52:30.44 Exhaust.select :close @17:52:30.54 SSV.dial CBV @17:52:30.56
CBV.dial SSV @17:52:31.33 Allowing 130s for 25.0ml of remaining 50.0ml @17:52:31.34 Intake.select :open @17:52:33.49 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:52:33.50 SS.reconfigure :inhaling 1.93ml/s @17:52:33.55 SS.seek 25ml @17:52:48.10 Vacuum too high -- waiting up to 40 seconds @17:52:48.11 SS.reconfigure :exhale @17:52:48.15 SS.hold @17:52:48.78 Intake @11.4psia, vacuum from 5.9 to 8.9psi @17:52:48.79 SS.reconfigure :inhaling 1.74ml/s @17:52:48.84 SS.seek 25ml @17:52:51.64 Stroke completed -- waiting for pressure to equalize ... @17:52:51.65 SS.reconfigure :exhale @17:52:51.69 SS.hold @17:52:51.99 Intake.select :close @17:52:52.13 SSV.dial :exhaust @17:52:52.86 Exhaust.select :open @17:52:52.97 SS.seek 0ml @17:53:01.83 Exhaust.select :close @17:53:01.93 SSV.dial CBV @17:53:01.95
CBV.dial SSV @17:53:02.69 Allowing 130s for 25.0ml of remaining 25.0ml @17:53:02.70 Intake.select :open @17:53:04.85 Intake @16.2psia, vacuum from 10.0 to 13.0psi @17:53:04.87 SS.reconfigure :inhaling 1.74ml/s @17:53:04.91 SS.seek 24.99ml @17:53:12.85 Vacuum too high -- waiting up to 40 seconds @17:53:12.86 SS.reconfigure :exhale @17:53:12.90 SS.hold @17:53:13.47 Intake @12.5psia, vacuum from 7.0 to 10.0psi @17:53:13.48 SS.reconfigure :inhaling 1.57ml/s @17:53:13.52 SS.seek 24.99ml @17:53:21.76 Vacuum too high -- waiting up to 40 seconds @17:53:21.77 SS.reconfigure :exhale @17:53:21.81 SS.hold @17:53:25.48 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:53:25.50 SS.reconfigure :inhaling 1.41ml/s @17:53:25.54 SS.seek 24.99ml @17:53:30.11 Stroke completed -- waiting for pressure to equalize ... @17:53:30.12 SS.reconfigure :exhale @17:53:30.16 SS.hold @17:53:32.10 Intake.select :close @17:53:32.23 SSV.dial :exhaust @17:53:32.97 Exhaust.select :open @17:53:33.07 SS.seek 0ml @17:53:42.25 Exhaust.select :close @17:53:42.35 Sampled 100.0ml @17:53:42.37 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:53:42.38 Intake.select :close @17:53:42.43 CSV.dial :waste @17:53:42.50 CS.seek 0ml @17:53:42.56 CSV.dial :air @17:53:42.60 SSV.dial CBV,avoiding: :exhaust @17:53:42.63
CBV.dial SSV @17:53:43.36 CS.seek 10ml @17:53:44.49
CTV.dial :air @17:53:45.24 delay 15 seconds @17:54:00.26 SSV.dial :spit,avoiding: :exhaust @17:54:01.57 CTV.dial CSV,avoiding: :intake @17:54:01.60
SS.seek 0ml @17:54:01.72 SSV.dial CBV,avoiding: :exhaust @17:54:06.76 CBV.dial between SSV and waste2 @17:54:07.14 CTV.dial CSV,avoiding: :air @17:54:07.17
CSV.dial CTV @17:54:07.73 CS.reconfigure :pressurize @17:54:07.77 CS.seek 0ml @17:54:28.93 BubblePoint: Intake @39.9psia, Outlet @15.7psia @17:54:28.94 CBV.dial SSV @17:54:29.26 SS.reconfigure :default @17:54:29.30 SS.seek 10ml @17:54:35.23 delay 15 seconds @17:54:50.35 Evacuated: Intake @16.8psia, Outlet @9.4psia @17:54:50.36 SSV.dial :spit,avoiding: :exhaust @17:54:51.67 SS.reconfigure :exhale @17:54:51.72 SS.seek 0ml @17:54:56.88 SS.reconfigure :fast @17:54:56.92 SS.seek 5ml @17:54:59.85 SSV.dial CBV,avoiding: :exhaust @17:54:59.89
CBV.dial SSV @17:55:01.17 CTV.dial :air,avoiding: :intake @17:55:02.47 CSV.dial CTV @17:55:02.51
SS.seek 10ml @17:55:03.50 CSV.dial :air @17:55:03.93 CS.reconfigure :fast @17:55:03.97 CS.seek 0ml @17:55:05.01
SSV.dial :spit,avoiding: :exhaust @17:55:06.35 SS.reconfigure :exhale @17:55:06.39 SS.seek 0ml @17:55:08.77 CS.stop @17:55:11.58
SS.reconfigure :fast @17:55:11.63 SS.seek 5ml @17:55:14.83 SSV.dial CBV,avoiding: :exhaust @17:55:14.86
CBV.dial SSV @17:55:17.18 SSV.dial :spit,avoiding: :exhaust @17:55:18.52 SS.reconfigure :default @17:55:18.56 SS.seek 0ml @17:55:22.07 SS.stop @17:55:22.11 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:55:22.12 Intake.select :close @17:55:22.19 CSV.dial :waste @17:55:22.30 SSV.dial CBV,avoiding: :exhaust @17:55:22.32
CBV.dial SSV @17:55:22.98 CS.seek 0ml @17:55:23.05 CSV.dial :air @17:55:23.64
CTV.dial :air @17:55:23.65 delay 15 seconds @17:55:23.79 CS.seek 10ml @17:55:38.67
SSV.dial :spit,avoiding: :exhaust @17:55:40.01 CTV.dial CSV,avoiding: :intake @17:55:40.04
SS.reconfigure :exhale @17:55:40.13 SS.seek 0ml @17:55:40.19 SSV.dial CBV,avoiding: :exhaust @17:55:47.04 CBV.dial between SSV and waste2 @17:55:47.42 CTV.dial CSV,avoiding: :air @17:55:47.45
CSV.dial CTV @17:55:48.01 CS.reconfigure :pressurize @17:55:48.05 CS.seek 0ml @17:56:10.45 BubblePoint: Intake @39.1psia, Outlet @14.8psia @17:56:10.46 CBV.dial SSV @17:56:10.79 SS.reconfigure :default @17:56:10.83 SS.seek 10ml @17:56:16.42 delay 15 seconds @17:56:31.54 Evacuated: Intake @17.3psia, Outlet @10.1psia @17:56:31.55 SSV.dial :spit,avoiding: :exhaust @17:56:32.90 SS.reconfigure :exhale @17:56:32.95 SS.seek 0ml @17:56:38.24 SS.reconfigure :fast @17:56:38.28 SS.seek 5ml @17:56:41.19 SSV.dial CBV,avoiding: :exhaust @17:56:41.21
CBV.dial SSV @17:56:42.51 CTV.dial :air,avoiding: :intake @17:56:43.82 CSV.dial CTV @17:56:43.85
SS.seek 10ml @17:56:44.84 CSV.dial :air @17:56:45.27 CS.reconfigure :fast @17:56:45.32 CS.seek 0ml @17:56:46.54
SSV.dial :spit,avoiding: :exhaust @17:56:47.88 SS.reconfigure :exhale @17:56:47.92 SS.seek 0ml @17:56:49.45 CS.stop @17:56:53.08
SS.reconfigure :fast @17:56:53.12 SS.seek 5ml @17:56:56.28 SSV.dial CBV,avoiding: :exhaust @17:56:56.30
CBV.dial SSV @17:56:58.63 SSV.dial :spit,avoiding: :exhaust @17:56:59.95 SS.reconfigure :default @17:56:59.99 SS.seek 0ml @17:57:03.07 SS.stop @17:57:03.10 Lysing: Adding 2.0ml AqMeOH @17:57:03.12 CBV.dial between CSV and waste1,avoiding: :DBV @17:57:04.56 CTV.dial CSV @17:57:05.86 CSV.dial CSR @17:57:06.65 Pulling 1ml of CSR.AqMeOH @17:57:06.66 CSR.select :AqMeOH @17:57:06.76 CS.reconfigure :default @17:57:06.81 CS.seek 1ml @17:57:10.17 delay 5 seconds @17:57:15.19 CSR.select :air @17:57:15.34 CS.seek 6ml @17:57:28.76 delay 2 seconds @17:57:30.78 CSV.dial :waste2 @17:57:31.21 CS.reconfigure :fast @17:57:31.25 CS.seek 0ml @17:57:45.36 CSV.dial CSR @17:57:45.85 Pulling 2ml of CSR.AqMeOH @17:57:45.86 CSR.select :AqMeOH @17:57:45.97 CS.reconfigure :default @17:57:46.01 CS.seek 2ml @17:57:51.86 delay 5 seconds @17:57:56.88 CSR.select :air @17:57:57.03 CS.seek 5ml @17:58:05.39 delay 2 seconds @17:58:07.40 CSV.dial CTV @17:58:07.83 CS.seek 1ml @17:58:18.96 Before lysing Collection Puck Heater at 18.7C/39.0psia @17:58:18.98 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @17:58:18.99 CH.seek 70C,8:00 @18:00:24.04 CH.hold 10:00,70C @18:00:24.22 Begun lysing Collection Puck Heater at 68C/32.1psia @18:10:24.23 Ended lysing Collection Puck Heater at 70C/15.1psia @18:10:24.24 CS.seek 0ml @18:10:27.64 CTV.dial between spare and air,avoiding: :intake @18:10:28.67 CSV.dial :air @18:10:29.13 CS.seek 5ml @18:10:42.52 CSV.dial CTV @18:10:43.09 delay 2 seconds @18:10:45.10 CSV.dial :air @18:10:45.54 delay 2 seconds @18:10:47.56 CSV.dial CTV @18:10:48.01 delay 2 seconds @18:10:50.03 CSV.dial :air @18:10:50.46 delay 2 seconds @18:10:52.48 CS.seek 0ml @18:11:05.92 Puck @14.7psia after final lysis pressurization @18:11:05.93 Recovering Lysate into Collection syringe @18:11:05.94 CSV.dial CBV @18:11:07.09 CBV.dial CSV @18:11:07.46 CS.seek 5ml @18:11:20.84 delay 5 seconds @18:11:25.85 CTV.dial :air @18:11:26.36 CS.seek 7.001ml @18:11:32.18 delay 5 seconds @18:11:37.20 CSV.dial :air2 @18:11:37.65 CS.seek 10ml @18:11:45.99 CSV.dial between air and CTV,avoiding: CBV @18:11:47.14 Lysing Complete @18:11:47.20 Storing sh1 puck in Collection Clamp to tube 1 @18:11:47.36 SC.reconfigure :tight @18:11:47.40 SC.seek 1 @18:11:51.02 SC.configure :limp @18:11:51.16 SE.reconfigure :place @18:11:51.23
Elbow.reconfigure :tight @18:11:51.27 Elbow.seek CC @18:11:51.29 SE.seek :beamBreak @18:11:51.43
Elbow.reconfigure :loose @18:11:51.52 Forearm.seek CC @18:11:52.54 Hand.close @18:11:53.33 CC.seek :open @18:11:54.15 SE.configure :default @18:11:54.19 SE.seek :place @18:11:57.76
Forearm.seek :retract @18:11:58.82 Elbow.reconfigure :tight @18:11:58.87 Elbow.seek 1 @18:12:00.62 Elbow.reconfigure :limp @18:12:00.66 Forearm.seek 1 @18:12:01.79 Elbow.hold @18:12:01.85 Elbow.reconfigure :tight @18:12:01.89 Hand.open @18:12:02.69 Forearm.force -20 @18:12:02.72 Forearm.seek 1 + 316 counts @18:12:03.11 Forearm.reconfigure :tight @18:12:03.15 Forearm.seek 1 + 700 counts @18:12:03.42 SE.seek :down @18:12:04.54
Forearm.reconfigure :loose @18:12:04.58 Forearm.seek :retract @18:12:06.20 SE.stop @18:12:06.24
Tranferring DA @18:12:06.25 Beginning samplePassC @18:12:06.27 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:12:06.44 Elbow.seek FlushPuck::Garage @18:12:08.22 Elbow.reconfigure :loose @18:12:08.31 Forearm.seek :garage @18:12:09.45 Hand.close @18:12:10.31 Forearm.seek :retract @18:12:11.42 Elbow.reconfigure :tight @18:12:11.46 Elbow.seek CC @18:12:14.39 Elbow.reconfigure :loose @18:12:14.48 Forearm.reconfigure :tight @18:12:14.52 Forearm.seek CC @18:12:16.28 CC.seek :closed @18:12:20.51 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.91 @18:12:20.58 Hand.open @18:12:21.38 Forearm.reconfigure :loose @18:12:21.42 Forearm.seek :retract @18:12:22.43 PSV.dial PSR @18:12:23.77 Pulling 1.5ml of PSR.flush @18:12:23.78 PSR.select :flush @18:12:23.90 PS.seek 1.5ml @18:12:39.95 delay 5 seconds @18:12:44.97 PSV.dial :waste1 @18:12:45.43 PS.seek 0ml @18:13:01.45 PSV.dial PSR @18:13:01.89 PSR.select :air @18:13:02.00 PS.seek 2.5ml @18:13:28.27 PRV.dial CBV,avoiding: :ab2 @18:13:28.31
CBV.dial PRV @18:13:29.95 PSV.dial :mixloop @18:13:30.97 CTV.dial :air,avoiding: :intake @18:13:30.98 PTV.dial :PRVmixing @18:13:32.24 PS.seek 0ml @18:13:58.39 PSV.dial :air @18:13:58.83 PS.seek 2.5ml @18:14:24.98 PSV.dial :mixloop @18:14:25.42 PS.seek 0ml @18:14:51.57 PSV.dial :air @18:14:52.02 PS.seek 2.5ml @18:15:18.16 PSV.dial :mixloop @18:15:18.61 PS.seek 0ml @18:15:44.85 Garaging FlushPuck in Collection Clamp @18:15:44.92 Elbow.reconfigure :tight @18:15:44.96 Elbow.seek CC @18:15:45.07 Elbow.reconfigure :loose @18:15:45.16 Forearm.seek CC @18:15:46.16 Hand.close @18:15:46.94 CC.seek :open @18:15:51.28 Forearm.seek :retract @18:15:52.36 Elbow.reconfigure :tight @18:15:52.40 Elbow.seek :garage @18:15:55.32 Elbow.reconfigure :loose @18:15:55.41 Forearm.reconfigure :tight @18:15:55.45 Forearm.seek :garage @18:15:56.56 Hand.open @18:15:57.37 Forearm.reconfigure :loose @18:15:57.41 Forearm.seek :retract @18:15:58.48 PSV.dial :mixloop @18:15:58.50 PTV.dial :PRVmixing @18:15:58.51 PRV.dial CBV @18:15:58.53
CBV.dial PRV @18:15:58.55 CTV.dial :air,avoiding: :intake @18:15:58.57 Loading lyfil puck from tube 2 into Collection Clamp @18:15:58.78 SC.reconfigure :tight @18:15:58.82 SC.seek 2 @18:16:02.39 SC.configure :limp @18:16:02.58 Elbow.reconfigure :tight @18:16:02.62 Elbow.seek 2 @18:16:05.38 Forearm.reconfigure :tight @18:16:05.43 Forearm.seek 2 + 700 counts @18:16:06.45 SE.reconfigure :pick @18:16:06.50 SE.seek :beamBreak @18:16:07.55 SE.configure :default @18:16:07.69 Elbow.seek 2 @18:16:07.85 Forearm.seek 2 + 300 counts @18:16:08.02 Forearm.reconfigure :loose @18:16:08.06 Hand.close @18:16:08.83 SE.seek :down @18:16:09.46
Forearm.seek :retract @18:16:09.53 SE.stop @18:16:10.46
Elbow.seek CC @18:16:12.09 Elbow.reconfigure :loose @18:16:12.18 Forearm.reconfigure :tight @18:16:12.22 Forearm.seek CC @18:16:13.54 CC.seek :closed @18:16:17.77 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.73 @18:16:17.78 Hand.open @18:16:18.62 Forearm.reconfigure :loose @18:16:18.66 Forearm.seek :retract @18:16:19.64 CTV.dial CSV,avoiding: :intake @18:16:20.94 CSV.dial CTV @18:16:21.24 PS.seek 2.5ml @18:16:47.47 CS.seek 5.5ml @18:16:59.73 delay 8 seconds @18:17:07.75 PSV.dial :air @18:17:08.19 delay 2 seconds @18:17:10.21 PSV.dial :waste2 @18:17:11.06 PS.seek 0ml @18:17:37.20 CBV.dial between PRV and waste1 @18:17:37.52 CSV.dial :air @18:17:37.97 CS.seek 10ml @18:17:50.20 CSV.dial CTV @18:17:50.64 CS.seek 0ml @18:18:16.67 CBV.dial :waste1 @18:18:16.96 delay 5 seconds @18:18:21.98 CTV.dial :air,avoiding: :intake @18:18:23.32 Storing lyfil puck in Collection Clamp to tube 1 @18:18:23.48 SC.reconfigure :tight @18:18:23.52 SC.seek 1 @18:18:27.11 SC.configure :limp @18:18:27.30 Elbow.reconfigure :tight @18:18:27.34 Elbow.seek 1 @18:18:29.11 Forearm.reconfigure :tight @18:18:29.15 Forearm.seek 1 + 700 counts @18:18:30.17 SE.reconfigure :pick @18:18:30.22 SE.seek :beamBreak @18:18:33.23 SE.configure :default @18:18:33.37 Elbow.reconfigure :limp @18:18:33.42 Forearm.reconfigure :loose @18:18:33.46 Forearm.seek 1 @18:18:33.87 Elbow.hold @18:18:33.93 Elbow.reconfigure :tight @18:18:34.02 SE.seek 0.18 Pucks below Top Plate @18:18:34.43 Forearm.force -20 @18:18:34.46 Forearm.seek 1 + 316 counts @18:18:34.75 Forearm.reconfigure :tight @18:18:34.80 Forearm.seek 1 + 700 counts @18:18:35.14 SE.seek 1.18 Pucks below Top Plate @18:18:35.62 Forearm.reconfigure :loose @18:18:35.67 Forearm.seek :retract @18:18:36.67 Elbow.seek CC @18:18:38.31 Elbow.reconfigure :loose @18:18:38.39 Forearm.seek CC @18:18:39.35 Hand.close @18:18:40.06 CC.seek :open @18:18:44.53 Forearm.seek :retract @18:18:45.58 Elbow.reconfigure :tight @18:18:45.62 Elbow.seek 1 @18:18:47.51 Elbow.reconfigure :limp @18:18:47.56 Forearm.seek 1 @18:18:49.12 Elbow.hold @18:18:49.18 Elbow.reconfigure :tight @18:18:49.22 Hand.open @18:18:50.04 Forearm.force -20 @18:18:50.08 Forearm.seek 1 + 318 counts @18:18:50.45 Forearm.reconfigure :tight @18:18:50.49 Forearm.seek 1 + 700 counts @18:18:50.75 SE.seek :down @18:18:51.87
Forearm.reconfigure :loose @18:18:51.91 Forearm.seek :retract @18:18:53.38 SE.stop @18:18:53.42
PSV.dial :air @18:18:54.24 PS.seek 2.5ml @18:19:20.47 delay 1 second @18:19:21.48 PSV.dial :mixloop @18:19:21.94 delay 1 second @18:19:22.95 PSV.dial :air @18:19:23.39 delay 1 second @18:19:24.41 PSV.dial :mixloop @18:19:24.85 delay 1 second @18:19:25.86 PSV.dial :air @18:19:26.32 delay 1 second @18:19:27.34 PS.seek 0ml @18:19:53.50 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:19:53.67 Elbow.seek FlushPuck::Garage @18:19:55.72 Elbow.reconfigure :loose @18:19:55.81 Forearm.seek :garage @18:19:56.88 Hand.close @18:19:57.54 Forearm.seek :retract @18:19:58.61 Elbow.reconfigure :tight @18:19:58.65 Elbow.seek PC @18:20:00.13 Elbow.reconfigure :loose @18:20:00.22 Forearm.reconfigure :tight @18:20:00.26 Forearm.seek PC @18:20:01.61 PC.seek :closed @18:20:05.47 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.45 @18:20:05.53 Hand.open @18:20:06.37 Forearm.reconfigure :loose @18:20:06.41 Forearm.seek :retract @18:20:07.48 PRV.dial :air @18:20:07.91 PSV.dial :mixloop @18:20:08.42 PS.seek 0.3ml @18:20:12.31 PSV.dial PSR @18:20:13.35 Pulling 0.4ml of PSR.air @18:20:13.36 PSR.select :air @18:20:13.39 PS.seek 0.7ml @18:20:18.37 delay 3 seconds @18:20:21.39 PSR.select :air @18:20:21.41 PS.seek 2.5ml @18:20:40.50 delay 3 seconds @18:20:43.51 PSV.dial :mixloop @18:20:44.50 PTV.dial :mixing @18:20:45.20 PBV.dial :waste2 @18:20:46.09 PS.seek 0ml @18:21:12.31 Garaging FlushPuck in Processing Clamp @18:21:12.38 Elbow.reconfigure :tight @18:21:12.42 Elbow.seek PC @18:21:12.69 Elbow.reconfigure :loose @18:21:12.77 Forearm.seek PC @18:21:13.71 Hand.close @18:21:14.31 PC.seek :open @18:21:18.26 Forearm.seek :retract @18:21:19.34 Elbow.reconfigure :tight @18:21:19.38 Elbow.seek :garage @18:21:20.98 Elbow.reconfigure :loose @18:21:21.07 Forearm.reconfigure :tight @18:21:21.11 Forearm.seek :garage @18:21:22.21 Hand.open @18:21:22.99 Forearm.reconfigure :loose @18:21:23.03 Forearm.seek :retract @18:21:24.52 Loading da puck from tube 2 into Processing Clamp @18:21:24.76 SC.reconfigure :tight @18:21:24.80 SC.seek 2 @18:21:28.39 SC.configure :limp @18:21:28.59 Elbow.reconfigure :tight @18:21:28.63 Elbow.seek 2 @18:21:30.68 Forearm.reconfigure :tight @18:21:30.72 Forearm.seek 2 + 700 counts @18:21:31.80 SE.reconfigure :pick @18:21:31.85 SE.seek :beamBreak @18:21:32.88 SE.configure :default @18:21:33.03 Elbow.seek 2 @18:21:33.20 Forearm.seek 2 + 300 counts @18:21:33.54 Forearm.reconfigure :loose @18:21:33.58 Hand.close @18:21:34.19 SE.seek :down @18:21:34.37
Forearm.seek :retract @18:21:34.97 SE.stop @18:21:35.40
Elbow.seek PC @18:21:38.03 Elbow.reconfigure :loose @18:21:38.12 Forearm.reconfigure :tight @18:21:38.16 Forearm.seek PC @18:21:39.53 PC.seek :closed @18:21:43.45 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.39 @18:21:43.46 Hand.open @18:21:44.23 Forearm.reconfigure :loose @18:21:44.28 Forearm.seek :retract @18:21:45.39 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @18:21:45.41 PH.seek 28C,5:00 @18:22:35.71 PH.hold 2:00:00,28C @18:22:35.76 delay 1:30 @18:24:05.80 PSR.select :air @18:24:05.81 PSV.dial PSR @18:24:06.99 Pulling 0.5ml of PSR.wash PSR.select :wash @18:24:07.11 PS.seek 0.5ml @18:24:13.03 delay 5 seconds @18:24:18.05 PSR.select :air @18:24:18.16 PS.seek 2ml @18:24:34.15 PTV.dial :puck @18:24:34.85 PSV.dial PTV @18:24:35.67 PBV.dial :waste1 @18:24:36.99 PS.seek 1.5ml @18:24:42.96 delay 3 seconds @18:24:45.98 PBV.dial between PSV and waste1 @18:24:46.35 PS.seek 0ml @18:25:02.34 delay 5:00 @18:30:02.36 recoverPSRreagent08 @18:30:02.37 PTV.dial :puck @18:30:02.38 PBV.dial :waste1 @18:30:02.74 delay 30 seconds @18:30:32.76 PBV.dial between PSV and waste1 @18:30:33.13 PSR.select :air @18:30:33.14 PSV.dial PSR @18:30:33.87 PS.reconfigure :fast @18:30:33.91 PS.seek 2.5ml @18:30:56.95 PSV.dial PTV @18:30:57.67 delay 5 seconds @18:31:02.69 PSV.dial PSR @18:31:03.42 delay 5 seconds @18:31:08.44 PSV.dial PTV @18:31:09.66 delay 5 seconds @18:31:14.67 PSV.dial :air @18:31:15.40 PS.seek 0ml @18:31:38.26 PTV.dial :PRVmixing,via: PRV @18:31:39.50 PSV.dial :mixloop @18:31:39.94 PRV.dial :ab1 @18:31:40.64 PS.reconfigure :slow @18:31:40.69 PS.seek 0.15ml @18:31:44.23 delay 2 seconds @18:31:46.25 CBV.dial PRV @18:31:46.27
PRV.dial CBV @18:31:46.84 PS.seek 0.34ml @18:31:51.26 delay 2 seconds @18:31:53.28 CBV.dial between PRV and PSV @18:31:53.57 PRV.dial :air @18:31:54.00 PS.seek 0.9ml @18:32:05.89 PRV.dial :waste2 @18:32:06.61 PS.seek 0.5ml @18:32:15.26 addPMLReagent08 @18:32:15.27 PSV.dial :air @18:32:15.72 PS.reconfigure :default @18:32:15.77 PS.seek 2ml @18:32:31.76 PSV.dial :PML @18:32:32.20 PTV.dial :mixing @18:32:32.92 PBV.dial :waste1 @18:32:33.32 PS.seek 1.85ml @18:32:35.67 PBV.dial between PSV and waste1 @18:32:36.05 PS.seek 0ml @18:32:55.60 delay 2 seconds @18:32:57.62 Beginning samplePassC @18:32:57.64 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:32:57.80 Elbow.reconfigure :tight @18:32:57.84 Elbow.seek FlushPuck::Garage @18:32:59.25 Elbow.reconfigure :loose @18:32:59.33 Forearm.seek :garage @18:33:00.42 Hand.close @18:33:01.14 Forearm.seek :retract @18:33:02.25 Elbow.reconfigure :tight @18:33:02.29 Elbow.seek CC @18:33:05.18 Elbow.reconfigure :loose @18:33:05.27 Forearm.reconfigure :tight @18:33:05.31 Forearm.seek CC @18:33:06.49 CC.seek :closed @18:33:10.73 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.83 @18:33:10.81 Hand.open @18:33:11.59 Forearm.reconfigure :loose @18:33:11.63 Forearm.seek :retract @18:33:12.61 PSV.dial PSR @18:33:13.66 Pulling 1.5ml of PSR.flush @18:33:13.67 PSR.select :flush @18:33:13.79 PS.seek 1.5ml @18:33:29.86 delay 5 seconds @18:33:34.88 PSV.dial :waste1 @18:33:35.31 PS.seek 0ml @18:33:51.32 PSV.dial PSR @18:33:51.87 PSR.select :air @18:33:51.98 PS.seek 2.5ml @18:34:18.11 PRV.dial CBV,avoiding: :ab2 @18:34:18.13
CBV.dial PRV @18:34:19.71 PSV.dial :mixloop @18:34:20.73 CTV.dial :air,avoiding: :intake @18:34:20.74 PTV.dial :PRVmixing @18:34:21.45 PS.seek 0ml @18:34:47.63 PSV.dial :air @18:34:48.08 PS.seek 2.5ml @18:35:14.29 PSV.dial :mixloop @18:35:14.73 PS.seek 0ml @18:35:41.01 PSV.dial :air @18:35:41.45 PS.seek 2.5ml @18:36:07.66 PSV.dial :mixloop @18:36:08.57 PS.seek 0ml @18:36:34.79 Garaging FlushPuck in Collection Clamp @18:36:34.86 Elbow.reconfigure :tight @18:36:34.90 Elbow.seek CC @18:36:35.17 Elbow.reconfigure :loose @18:36:35.26 Forearm.seek CC @18:36:36.21 Hand.close @18:36:36.91 CC.seek :open @18:36:41.25 Forearm.seek :retract @18:36:42.29 Elbow.reconfigure :tight @18:36:42.33 Elbow.seek :garage @18:36:45.34 Elbow.reconfigure :loose @18:36:45.43 Forearm.reconfigure :tight @18:36:45.48 Forearm.seek :garage @18:36:46.63 Hand.open @18:36:47.46 Forearm.reconfigure :loose @18:36:47.50 Forearm.seek :retract @18:36:48.54 delayUntil "18:40:57.61" @18:36:48.57 Whole Cell Archive sampling 100ml @18:36:48.59 Loading wcr puck from tube 2 into Collection Clamp @18:36:49.03 Elbow.reconfigure :tight @18:36:49.07 Elbow.seek 2 @18:36:51.04 Forearm.reconfigure :tight @18:36:51.09 Forearm.seek 2 + 700 counts @18:36:52.08 SE.reconfigure :pick @18:36:52.14 SE.seek :beamBreak @18:36:53.30 SE.configure :default @18:36:53.46 Elbow.seek 2 @18:36:53.86 Forearm.seek 2 + 300 counts @18:36:54.10 Forearm.reconfigure :loose @18:36:54.15 Hand.close @18:36:54.91 SE.seek :down @18:36:55.12 Forearm.seek :retract @18:36:55.73 SE.stop @18:36:56.07 Elbow.seek CC @18:36:57.61 Elbow.reconfigure :loose @18:36:57.70 Forearm.reconfigure :tight @18:36:57.75 Forearm.seek CC @18:36:59.01 CC.seek :closed @18:37:03.24 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.77 @18:37:03.25 Hand.open @18:37:04.08 Forearm.reconfigure :loose @18:37:04.13 Forearm.seek :retract @18:37:05.17 collectSample 100ml w/bubble point of 35psi @18:37:05.18 Priming 7ml @18:37:05.19 Intake.select :close @18:37:05.20 Exhaust.select :close @18:37:05.21 SS.hold @18:37:05.40 Calibrating pressure sensors @18:37:05.42 CTV.dial :air @18:37:05.43 SSV.dial :spit,avoiding: :exhaust @18:37:05.45 SS.reconfigure :fast @18:37:05.49 SS.seek 5ml @18:37:08.33 SSV.dial CBV @18:37:08.36 CBV.dial SSV @18:37:09.67 delay 3.8 seconds @18:37:13.59 SSV.dial :spit @18:37:14.91 SS.seek 0ml @18:37:17.68 SSV.dial CBV @18:37:18.99 CTV.dial :intake @18:37:20.20 SSV.dial CBV @18:37:20.23 CBV.dial SSV @18:37:20.24 Allowing 40s for 7.0ml of remaining 7.0ml @18:37:20.25 Intake.select :open @18:37:22.41 Intake @16.1psia, vacuum from 10.0 to 13.0psi @18:37:22.43 SS.reconfigure :inhaling 2.14ml/s @18:37:22.47 SS.seek 7ml @18:37:29.18 Stroke completed -- waiting for pressure to equalize ... @18:37:29.19 SS.reconfigure :exhale @18:37:29.24 SS.hold @18:37:32.71 Intake.select :close @18:37:32.85 SSV.dial :exhaust @18:37:33.59 Exhaust.select :open @18:37:33.70 SS.seek 0ml @18:37:37.90 Exhaust.select :close @18:37:38.01 Sampling 100.0ml @18:37:38.02 Intake.select :close @18:37:38.03 Exhaust.select :close @18:37:38.04 SS.reconfigure :default @18:37:38.08 SS.hold @18:37:38.27 CTV.dial :intake @18:37:38.29 SSV.dial CBV @18:37:38.31 CBV.dial SSV @18:37:39.06 Allowing 130s for 25.0ml of remaining 100.0ml @18:37:39.07 Intake.select :open @18:37:41.22 Intake @16.2psia, vacuum from 10.0 to 13.0psi @18:37:41.24 SS.reconfigure :inhaling 2.14ml/s @18:37:41.29 SS.seek 25ml @18:37:46.70 Vacuum too high -- waiting up to 40 seconds @18:37:46.72 SS.reconfigure :exhale @18:37:46.76 SS.hold @18:37:47.48 Intake @12.0psia, vacuum from 6.5 to 9.5psi @18:37:47.50 SS.reconfigure :inhaling 1.93ml/s @18:37:47.54 SS.seek 25ml @18:37:58.49 Stroke completed -- waiting for pressure to equalize ... @18:37:58.50 SS.reconfigure :exhale @18:37:58.55 SS.hold @18:38:01.45 Intake.select :close @18:38:01.59 SSV.dial :exhaust @18:38:02.34 Exhaust.select :open @18:38:02.45 SS.seek 0ml @18:38:11.59 Exhaust.select :close @18:38:11.69 SSV.dial CBV @18:38:11.71 CBV.dial SSV @18:38:12.46 Allowing 130s for 25.0ml of remaining 75.0ml @18:38:12.48 Intake.select :open @18:38:14.63 Intake @16.1psia, vacuum from 10.0 to 13.0psi @18:38:14.65 SS.reconfigure :inhaling 1.93ml/s @18:38:14.69 SS.seek 25ml @18:38:20.98 Vacuum too high -- waiting up to 40 seconds @18:38:21.00 SS.reconfigure :exhale @18:38:21.04 SS.hold @18:38:21.65 Intake @11.7psia, vacuum from 6.2 to 9.2psi @18:38:21.67 SS.reconfigure :inhaling 1.74ml/s @18:38:21.71 SS.seek 25ml @18:38:32.72 Stroke completed -- waiting for pressure to equalize ... @18:38:32.74 SS.reconfigure :exhale @18:38:32.78 SS.hold @18:38:34.73 Intake.select :close @18:38:34.88 SSV.dial :exhaust @18:38:35.60 Exhaust.select :open @18:38:35.71 SS.seek 0ml @18:38:44.60 Exhaust.select :close @18:38:44.71 SSV.dial CBV @18:38:44.73 CBV.dial SSV @18:38:45.49 Allowing 130s for 25.0ml of remaining 50.0ml @18:38:45.51 Intake.select :open @18:38:47.66 Intake @16.1psia, vacuum from 10.0 to 13.0psi @18:38:47.68 SS.reconfigure :inhaling 1.74ml/s @18:38:47.72 SS.seek 25ml @18:38:57.67 Vacuum too high -- waiting up to 40 seconds @18:38:57.68 SS.reconfigure :exhale @18:38:57.73 SS.hold @18:38:58.30 Intake @12.1psia, vacuum from 6.6 to 9.6psi @18:38:58.32 SS.reconfigure :inhaling 1.57ml/s @18:38:58.36 SS.seek 25ml @18:39:07.45 Stroke completed -- waiting for pressure to equalize ... @18:39:07.46 SS.reconfigure :exhale @18:39:07.51 SS.hold @18:39:11.70 Intake.select :close @18:39:11.84 SSV.dial :exhaust @18:39:12.59 Exhaust.select :open @18:39:12.70 SS.seek 0ml @18:39:21.75 Exhaust.select :close @18:39:21.86 SSV.dial CBV @18:39:21.88 CBV.dial SSV @18:39:22.63 Allowing 130s for 25.0ml of remaining 25.0ml @18:39:22.65 Intake.select :open @18:39:24.80 Intake @16.1psia, vacuum from 10.0 to 13.0psi @18:39:24.82 SS.reconfigure :inhaling 1.57ml/s @18:39:24.86 SS.seek 25ml @18:39:30.36 Vacuum too high -- waiting up to 40 seconds @18:39:30.37 SS.reconfigure :exhale @18:39:30.41 SS.hold @18:39:31.52 Intake @13.9psia, vacuum from 8.4 to 11.4psi @18:39:31.54 SS.reconfigure :inhaling 1.41ml/s @18:39:31.58 SS.seek 25ml @18:39:37.81 Vacuum too high -- waiting up to 40 seconds @18:39:38.03 SS.reconfigure :exhale @18:39:38.07 SS.hold @18:39:40.91 Intake @14.6psia, vacuum from 9.1 to 12.1psi @18:39:40.93 SS.reconfigure :inhaling 1.27ml/s @18:39:40.97 SS.seek 25ml @18:39:46.48 Vacuum too high -- waiting up to 40 seconds @18:39:46.49 SS.reconfigure :exhale @18:39:46.54 SS.hold @18:39:51.42 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:39:51.44 SS.reconfigure :inhaling 1.14ml/s @18:39:51.49 SS.seek 25ml @18:39:58.36 Vacuum too high -- waiting up to 40 seconds @18:39:58.37 SS.reconfigure :exhale @18:39:58.41 SS.hold @18:40:04.92 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:40:04.94 SS.reconfigure :inhaling 1.03ml/s @18:40:04.98 SS.seek 25ml @18:40:06.75 Stroke completed -- waiting for pressure to equalize ... @18:40:06.76 SS.reconfigure :exhale @18:40:06.81 SS.hold @18:40:07.66 Intake.select :close @18:40:07.81 SSV.dial :exhaust @18:40:08.55 Exhaust.select :open @18:40:08.66 SS.seek 0ml @18:40:17.57 Exhaust.select :close @18:40:17.67 Sampled 100.0ml @18:40:17.69 Drying puck before fixing for 20:00 @18:40:17.70 CBV.dial :waste2b @18:40:18.42 delay 1:00 @18:40:57.63 recoverPRVreagent08 @18:40:57.64 PTV.dial :mixing @18:40:58.36 PBV.dial :waste1 @18:40:58.72 delay 30 seconds @18:41:18.44 CSV.dial :air @18:41:18.90 CS.reconfigure :fast @18:41:19.44 CS.seek 10ml @18:41:28.74 PBV.dial between PSV and waste1 @18:41:29.09 PSV.dial :air @18:41:29.55 PS.reconfigure :fast @18:41:29.59 PS.seek 2.5ml @18:41:42.38 Pumping wcr puck from tube 2 up to 35psi... @18:41:42.40 CBV.dial SSV @18:41:43.69 CBV.dial between CSV and waste2b @18:41:44.62 CSV.dial between air and CTV @18:41:45.06 CS.seek 7.214ml @18:41:52.14 CTV.dial CSV,avoiding: :air @18:41:52.17 CSV.dial CTV @18:41:52.93 CS.reconfigure :pressurize @18:41:53.01 CS.seek 0ml @18:41:53.02 PSV.dial :mixloop @18:41:53.51 delay 2 seconds @18:41:55.53 PSV.dial :air @18:41:55.98 delay 2 seconds @18:41:58.00 PSV.dial :mixloop @18:41:58.45 delay 2 seconds @18:42:00.47 PSV.dial :air @18:42:00.92 PS.seek 0ml @18:42:09.93 CBV.dial :waste2b @18:42:10.26 CS.reconfigure :default @18:42:10.31 CS.seek 0ml @18:42:12.82 delay 1:00 @18:42:24.15 washCycleDA #1 of 2 @18:42:24.16 PSR.select :air @18:42:24.18 PSV.dial PSR @18:42:25.50 Pulling 0.5ml of PSR.wash @18:42:25.51 PSR.select :wash @18:42:25.62 PS.reconfigure :default @18:42:25.67 PS.seek 0.5ml @18:42:31.62 delay 5 seconds @18:42:36.64 PSR.select :air @18:42:36.75 PS.seek 2ml @18:42:52.84 PTV.dial :puck @18:42:53.56 PSV.dial PTV @18:42:54.28 PBV.dial :waste1 @18:42:54.69 PS.seek 1.5ml @18:43:00.56 delay 3 seconds @18:43:03.58 PBV.dial between PSV and waste1 @18:43:03.96 PS.seek 0ml @18:43:12.84 CTV.dial :air,avoiding: :intake @18:43:14.13 Fix #1 of 2 (with RNAlater) for 10:00 @18:43:14.15 CSV.dial CSR @18:43:14.64 Pulling 2ml of CSR.RNAlater @18:43:14.65 CSR.select :RNAlater @18:43:14.76 CS.seek 2ml @18:43:20.03 delay 2:00 @18:43:20.69 delay 5 seconds @18:43:25.71 CSR.select :air @18:43:25.82 CS.reconfigure :fast @18:43:25.86 CS.seek 10ml @18:43:44.57 Pumping wcr puck from tube 2 up to 35psi... @18:43:44.58 CBV.dial SSV @18:43:45.85 CBV.dial between CSV and waste2b @18:43:46.78 CSV.dial between air and CTV @18:43:47.39 CS.seek 7.374ml @18:43:54.07 CTV.dial CSV,avoiding: :air @18:43:54.09 CSV.dial CTV @18:43:55.46 CS.reconfigure :pressurize @18:43:55.51 CS.seek 0ml @18:44:13.62 delay 10:00 @18:45:20.05 recoverPSRreagent08 @18:45:20.06 PTV.dial :puck @18:45:20.07 PBV.dial :waste1 @18:45:20.44 delay 30 seconds @18:45:50.47 PBV.dial between PSV and waste1 @18:45:50.82 PSR.select :air @18:45:50.84 PSV.dial PSR @18:45:51.57 PS.reconfigure :fast @18:45:51.62 PS.seek 2.5ml @18:46:14.86 PSV.dial PTV @18:46:15.60 delay 5 seconds @18:46:20.62 PSV.dial PSR @18:46:21.35 delay 5 seconds @18:46:26.37 PSV.dial PTV @18:46:27.10 delay 5 seconds @18:46:32.12 PSV.dial :air @18:46:32.85 PS.seek 0ml @18:46:55.99 washCycleDA #2 of 2 @18:46:56.00 PSR.select :air @18:46:56.02 PSV.dial PSR @18:46:57.35 Pulling 0.5ml of PSR.wash @18:46:57.36 PSR.select :wash @18:46:57.48 PS.reconfigure :default @18:46:57.52 PS.seek 0.5ml @18:47:03.43 delay 5 seconds @18:47:08.45 PSR.select :air @18:47:08.56 PS.seek 2ml @18:47:24.61 PTV.dial :puck @18:47:24.62 PSV.dial PTV @18:47:25.33 PBV.dial :waste1 @18:47:25.75 PS.seek 1.5ml @18:47:31.58 delay 3 seconds @18:47:34.60 PBV.dial between PSV and waste1 @18:47:34.97 PS.seek 0ml @18:47:50.96 delay 2:00 @18:49:50.98 recoverPSRreagent08 @18:49:50.99 PTV.dial :puck @18:49:51.00 PBV.dial :waste1 @18:49:51.36 delay 30 seconds @18:50:21.38 PBV.dial between PSV and waste1 @18:50:21.75 PSR.select :air @18:50:21.77 PSV.dial PSR @18:50:22.51 PS.reconfigure :fast @18:50:22.55 PS.seek 2.5ml @18:50:45.67 PSV.dial PTV @18:50:46.40 delay 5 seconds @18:50:51.42 PSV.dial PSR @18:50:52.15 delay 5 seconds @18:50:57.17 PSV.dial PTV @18:50:58.01 delay 5 seconds @18:51:03.03 PSV.dial :air @18:51:03.78 PS.seek 0ml @18:51:26.98 getPMLReagent08 @18:51:26.99 PRV.dial :air @18:51:27.42 delay 5 seconds @18:51:32.44 PTV.dial :PRVmixing @18:51:33.70 delay 5 seconds @18:51:38.72 PRV.dial :ab2 @18:51:39.17 PTV.dial :PRVmixing @18:51:39.18 PSV.dial :mixloop @18:51:39.63 PS.reconfigure :default @18:51:39.67 PS.seek 0.3ml @18:51:43.57 delay 2 seconds @18:51:45.59 PRV.dial :air @18:51:46.10 PS.seek 0.55ml @18:51:49.42 addPMLReagent08 @18:51:49.43 PSV.dial :air @18:51:49.88 PS.seek 2ml @18:52:05.35 PSV.dial :PML @18:52:05.81 PTV.dial :mixing @18:52:06.52 PBV.dial :waste1 @18:52:06.94 PS.seek 1.85ml @18:52:09.35 PBV.dial between PSV and waste1 @18:52:09.74 PS.seek 0ml @18:52:29.42 delay 2 seconds @18:52:31.44 delay 8:00 @18:54:13.64 CBV.dial :waste2b @18:54:13.96 CS.reconfigure :default @18:54:14.01 CS.seek 0ml @18:54:15.74 delay 1:00 @18:55:15.76 CTV.dial :air,avoiding: :intake @18:55:17.05 Fix #2 of 2 (with RNAlater) for 10:00 @18:55:17.07 CSV.dial CSR @18:55:17.56 Pulling 2ml of CSR.RNAlater @18:55:17.57 CSR.select :RNAlater @18:55:17.68 CS.seek 2ml @18:55:23.58 delay 5 seconds @18:55:28.60 CSR.select :air @18:55:28.71 CS.reconfigure :fast @18:55:28.75 CS.seek 10ml @18:55:47.36 Pumping wcr puck from tube 2 up to 35psi... @18:55:47.37 CBV.dial SSV @18:55:48.64 CBV.dial between CSV and waste2b @18:55:49.57 CSV.dial between air and CTV @18:55:50.18 CS.seek 7.386ml @18:55:56.84 CTV.dial CSV,avoiding: :air @18:55:56.86 CSV.dial CTV @18:55:58.19 CS.reconfigure :pressurize @18:55:58.24 CS.seek 0ml @18:56:16.23 delay 10:00 @19:00:31.46 recoverPRVreagent08 @19:00:31.47 PTV.dial :mixing @19:00:31.48 PBV.dial :waste1 @19:00:31.84 delay 30 seconds @19:01:01.87 PBV.dial between PSV and waste1 @19:01:02.23 PSV.dial :air @19:01:02.69 PS.reconfigure :fast @19:01:02.73 PS.seek 2.5ml @19:01:26.06 PSV.dial :mixloop @19:01:26.50 delay 2 seconds @19:01:28.51 PSV.dial :air @19:01:28.96 delay 2 seconds @19:01:30.98 PSV.dial :mixloop @19:01:31.43 delay 2 seconds @19:01:33.45 PSV.dial :air @19:01:34.36 PS.seek 0ml @19:01:57.56 washCycleDA #1 of 3 @19:01:57.57 PSR.select :air @19:01:57.59 PSV.dial PSR @19:01:58.91 Pulling 0.5ml of PSR.wash @19:01:58.92 PSR.select :wash @19:01:59.03 PS.reconfigure :default @19:01:59.08 PS.seek 0.5ml @19:02:05.06 delay 5 seconds @19:02:10.08 PSR.select :air @19:02:10.20 PS.seek 2ml @19:02:26.18 PTV.dial :puck @19:02:26.93 PSV.dial PTV @19:02:27.65 PBV.dial :waste1 @19:02:28.07 PS.seek 1.5ml @19:02:33.96 delay 3 seconds @19:02:36.98 PBV.dial between PSV and waste1 @19:02:37.35 PS.seek 0ml @19:02:53.34 delay 2:00 @19:04:53.37 recoverPSRreagent08 PTV.dial :puck @19:04:53.38 PBV.dial :waste1 @19:04:53.74 delay 30 seconds @19:05:23.76 PBV.dial between PSV and waste1 @19:05:24.13 PSR.select :air @19:05:24.15 PSV.dial PSR @19:05:24.99 PS.reconfigure :fast @19:05:25.04 PS.seek 2.5ml @19:05:48.33 PSV.dial PTV @19:05:49.16 delay 5 seconds @19:05:54.18 PSV.dial PSR @19:05:55.00 delay 5 seconds @19:06:00.02 PSV.dial PTV @19:06:00.72 delay 5 seconds @19:06:05.74 PSV.dial :air @19:06:06.48 PS.seek 0ml @19:06:16.25 CBV.dial :waste2b @19:06:16.58 CS.reconfigure :default @19:06:16.63 CS.seek 0ml @19:06:18.40 delay 1:00 @19:06:29.68 washCycleDA #2 of 3 @19:06:29.69 PSR.select :air @19:06:29.70 PSV.dial PSR @19:06:31.02 Pulling 0.5ml of PSR.wash @19:06:31.03 PSR.select :wash @19:06:31.15 PS.reconfigure :default @19:06:31.19 PS.seek 0.5ml @19:06:37.15 delay 5 seconds @19:06:42.16 PSR.select :air @19:06:42.28 PS.seek 2ml @19:06:58.23 PTV.dial :puck @19:06:58.25 PSV.dial PTV @19:06:58.95 PBV.dial :waste1 @19:06:59.39 PS.seek 1.5ml @19:07:05.23 delay 3 seconds @19:07:08.25 PBV.dial between PSV and waste1 @19:07:08.63 PS.seek 0ml @19:07:18.43 CTV.dial :air,avoiding: :intake @19:07:24.64 delay 2:00 @19:09:24.67 recoverPSRreagent08 @19:09:24.68 PTV.dial :puck @19:09:24.69 PBV.dial :waste1 @19:09:25.06 delay 30 seconds @19:09:55.09 PBV.dial between PSV and waste1 @19:09:55.44 PSR.select :air @19:09:55.45 PSV.dial PSR @19:09:56.19 PS.reconfigure :fast @19:09:56.24 PS.seek 2.5ml @19:10:19.54 PSV.dial PTV @19:10:20.28 delay 5 seconds @19:10:25.30 PSV.dial PSR @19:10:26.03 delay 5 seconds @19:10:31.05 PSV.dial PTV @19:10:31.78 delay 5 seconds @19:10:36.80 PSV.dial :air @19:10:37.52 PS.seek 0ml @19:11:00.98 washCycleDA #3 of 3 @19:11:00.99 PSR.select :air @19:11:01.01 PSV.dial PSR @19:11:02.33 Pulling 0.5ml of PSR.wash @19:11:02.34 PSR.select :wash @19:11:02.45 PS.reconfigure :default @19:11:02.49 PS.seek 0.5ml @19:11:08.36 delay 5 seconds @19:11:13.37 PSR.select :air @19:11:13.49 PS.seek 2ml @19:11:29.45 PTV.dial :puck @19:11:29.46 PSV.dial PTV @19:11:30.16 PBV.dial :waste1 @19:11:30.57 PS.seek 1.5ml @19:11:36.45 delay 3 seconds @19:11:39.46 PBV.dial between PSV and waste1 @19:11:39.83 PS.seek 0ml @19:11:55.82 delay 2:00 @19:13:55.85 recoverPSRreagent08 @19:13:55.86 PTV.dial :puck @19:13:55.87 PBV.dial :waste1 @19:13:56.22 delay 30 seconds @19:14:26.25 PBV.dial between PSV and waste1 @19:14:26.60 PSR.select :air @19:14:26.62 PSV.dial PSR @19:14:27.35 PS.reconfigure :fast @19:14:27.40 PS.seek 2.5ml @19:14:50.69 PSV.dial PTV @19:14:51.43 delay 5 seconds @19:14:56.45 PSV.dial PSR @19:14:57.18 delay 5 seconds @19:15:02.20 PSV.dial PTV @19:15:02.93 delay 5 seconds @19:15:08.35 PSV.dial :air @19:15:09.14 PS.seek 0ml @19:15:32.56 Gate.power :camera,:ON @19:15:32.58 Mixing DA Substrates @19:15:32.59 PSR.select :air @19:15:32.60 PSV.dial PSR @19:15:34.16 Pulling 0.25ml of PSR.sub1 @19:15:34.21 PSR.select :sub1 @19:15:34.51 PS.reconfigure :default @19:15:34.68 PS.seek 0.25ml @19:15:38.17 delay 5 seconds @19:15:43.24 Pulling 0.25ml of PSR.sub2 @19:15:43.25 PSR.select :sub2 @19:15:43.36 PS.seek 0.5ml @19:15:46.79 delay 5 seconds @19:15:51.80 PSR.select :air @19:15:51.91 PS.seek 2ml @19:16:08.00 PTV.dial :puck @19:16:08.01 PSV.dial PTV @19:16:08.74 PBV.dial :waste1 @19:16:09.17 PS.seek 1.5ml @19:16:15.00 delay 3 seconds @19:16:18.02 PBV.dial between PSV and waste1 @19:16:18.40 PS.seek 0ml @19:16:34.63 delay 10 seconds @19:16:44.65 recoverPSRreagent08 @19:16:44.66 PTV.dial :puck @19:16:44.67 PBV.dial :waste1 @19:16:45.03 delay 30 seconds @19:17:15.06 PBV.dial between PSV and waste1 @19:17:15.42 PSR.select :air @19:17:15.44 PSV.dial PSR @19:17:16.30 PS.reconfigure :fast @19:17:16.35 PS.seek 2.5ml @19:17:39.93 PSV.dial PTV @19:17:40.66 delay 5 seconds @19:17:45.68 PSV.dial PSR @19:17:46.41 delay 5 seconds @19:17:51.43 PSV.dial PTV @19:17:52.16 delay 5 seconds @19:17:57.17 PSV.dial :air @19:17:57.91 PS.seek 0ml @19:18:21.28 Additional air clear @19:18:21.29 PSR.select :air @19:18:21.31 PSV.dial PSR @19:18:22.64 Pulling 2ml of PSR.air @19:18:22.65 PSR.select :air @19:18:22.68 PS.reconfigure :default @19:18:22.72 PS.seek 2ml @19:18:43.90 delay 5 seconds @19:18:48.92 PTV.dial :puck @19:18:48.94 PSV.dial PTV @19:18:49.67 PBV.dial :waste1 @19:18:50.09 PS.seek 1.5ml @19:18:55.97 delay 3 seconds @19:18:58.98 PBV.dial between PSV and waste1 @19:18:59.36 delay 1 second @19:19:00.38 PSV.dial :waste1 @19:19:01.40 PS.seek 0ml @19:19:17.45 Processing Puck Heater at 28C before imaging @19:19:17.46 PH.coast @19:19:17.59 Imaging da puck in Processing Clamp while storing it to tube 1 @19:19:17.74 SC.reconfigure :tight @19:19:17.79 SC.seek 1 @19:19:21.44 SC.configure :limp @19:19:21.63 Elbow.reconfigure :tight @19:19:21.68 Elbow.seek 1 @19:19:23.69 Forearm.reconfigure :tight @19:19:23.73 Forearm.seek 1 + 700 counts @19:19:24.78 SE.reconfigure :pick @19:19:24.83 SE.seek :beamBreak @19:19:27.88 SE.configure :default @19:19:28.02 Elbow.reconfigure :limp @19:19:28.07 Forearm.reconfigure :loose @19:19:28.11 Forearm.seek 1 @19:19:28.48 Elbow.hold @19:19:28.55 Elbow.reconfigure :tight @19:19:28.64 SE.seek 1.19 Pucks below Top Plate @19:19:29.20 Forearm.force -20 @19:19:29.24 Forearm.seek 1 + 319 counts @19:19:29.55 Forearm.reconfigure :tight @19:19:29.60 Forearm.seek 1 + 700 counts @19:19:29.80 SE.seek 2.19 Pucks below Top Plate @19:19:30.26 Forearm.reconfigure :loose @19:19:30.30 Forearm.seek :retract @19:19:31.28 Elbow.seek PC @19:19:33.76 Elbow.reconfigure :loose @19:19:33.85 Forearm.seek PC @19:19:34.92 Hand.close @19:19:35.69 PC.seek :open @19:19:39.72 Forearm.seek :retract @19:19:40.81 Elbow.reconfigure :tight @19:19:40.86 Elbow.seek 1 @19:19:43.94 Forearm.reconfigure :tight @19:19:43.98 Forearm.seek 1 + 700 counts @19:19:44.95 Hand.open @19:19:45.75 SE.seek 1.84 Pucks below Top Plate @19:19:46.11 SE.reconfigure :seal @19:19:46.16 SE.seek 0.73 Pucks below Top Plate @19:19:46.76 Lights, camera, ACTION! @19:19:46.79 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22oct0319h100ml.tif @19:20:02.38 SX MX5/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 11.862 seconds /var/log/esp/da22oct0319h100ml.tif: TIFF Upload Complete @19:20:02.44 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22oct0319h100ml15s.tif @19:20:06.21 Waiting >20 seconds for da to relinquish FlushPuck @19:20:18.14 SX MX5/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22oct0319h100ml15s.tif: TIFF Upload Complete @19:20:18.17 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da22oct0319h100ml25s.tif @19:20:43.90 SX MX5/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da22oct0319h100ml25s.tif: TIFF Upload Complete @19:20:43.92 Gate.power :camera,:OFF @19:20:43.94 DAfans.email "11.862 sec auto-exposure",Subject: "da22oct0319h100ml images!" @19:20:44.25 Elbow.seek 1 @19:20:44.43 Forearm.seek 1 + 700 counts @19:20:44.52 SE.reconfigure :gently @19:20:44.56 SE.seek 1.44 Pucks below Top Plate @19:20:45.27 Elbow.reconfigure :limp @19:20:45.32 Forearm.reconfigure :loose @19:20:45.38 Forearm.seek 1 @19:20:45.78 Elbow.hold @19:20:45.85 Elbow.reconfigure :tight @19:20:45.92 SE.reconfigure :default @19:20:45.97 SE.seek 1.63 Pucks below Top Plate @19:20:46.40 Forearm.force -20 @19:20:46.44 Forearm.seek 1 + 317 counts @19:20:46.77 Forearm.reconfigure :tight @19:20:46.82 Forearm.seek 1 + 700 counts @19:20:47.23 SE.seek :down @19:20:48.48 Forearm.reconfigure :loose @19:20:48.58 Forearm.seek :retract @19:20:49.83 SE.stop @19:20:49.93 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:20:50.44 Elbow.seek FlushPuck::Garage @19:20:52.56 Elbow.reconfigure :loose @19:20:52.75 Forearm.seek :garage @19:20:53.94 Hand.close @19:20:54.96 Forearm.seek :retract @19:20:56.10 Elbow.reconfigure :tight @19:20:56.19 Elbow.seek PC @19:20:57.57 Elbow.reconfigure :loose @19:20:57.79 Forearm.reconfigure :tight @19:20:57.90 Forearm.seek PC @19:20:59.60 PC.seek :closed @19:21:03.66 Processing Clamp onSpring/offSpring for 9/24 samples, on/off torque 1.43 @19:21:03.83 Hand.open @19:21:04.71 Forearm.reconfigure :loose @19:21:04.83 Forearm.seek :retract @19:21:06.01 PSR.select :air @19:21:06.06 Storing wcr puck in Collection Clamp to tube 1 @19:21:06.10 PSV.dial PSR @19:21:08.06 Pulling 0.5ml of PSR.flush @19:21:08.10 PSR.select :flush @19:21:08.32 Elbow.reconfigure :tight @19:21:08.43 Elbow.seek 1 @19:21:08.54 PS.seek 0.5ml @19:21:11.36 Forearm.reconfigure :tight @19:21:11.45 Forearm.seek 1 + 700 counts @19:21:12.74 SE.reconfigure :pick @19:21:12.84 SE.seek :beamBreak @19:21:14.53 delay 5 seconds @19:21:15.98 SE.configure :default @19:21:16.29 Elbow.reconfigure :limp @19:21:16.38 Forearm.reconfigure :loose @19:21:16.46 Forearm.seek 1 @19:21:16.95 Elbow.hold @19:21:17.08 Elbow.reconfigure :tight @19:21:17.31 SE.seek 2.19 Pucks below Top Plate @19:21:17.90 Forearm.force -20 @19:21:18.05 Forearm.seek 1 + 316 counts @19:21:18.56 Forearm.reconfigure :tight @19:21:18.65 Forearm.seek 1 + 700 counts @19:21:18.97 SE.seek 3.19 Pucks below Top Plate @19:21:19.62 Forearm.reconfigure :loose @19:21:19.72 Forearm.seek :retract @19:21:19.77 PSR.select :air @19:21:20.01 PS.seek 2.5ml @19:21:20.77 Elbow.seek CC @19:21:22.51 Elbow.reconfigure :loose @19:21:22.70 Forearm.seek CC @19:21:23.74 Hand.close @19:21:24.59 CC.seek :open @19:21:29.32 Forearm.seek :retract @19:21:30.52 Elbow.reconfigure :tight @19:21:30.61 Elbow.seek 1 @19:21:32.46 Elbow.reconfigure :limp @19:21:32.55 Forearm.seek 1 @19:21:33.73 Elbow.hold @19:21:33.87 Elbow.reconfigure :tight @19:21:33.95 Hand.open @19:21:34.97 Forearm.force -20 @19:21:35.05 Forearm.seek 1 + 317 counts @19:21:35.57 Forearm.reconfigure :tight @19:21:35.66 Forearm.seek 1 + 700 counts @19:21:36.11 SE.seek :down @19:21:37.35 Forearm.reconfigure :loose @19:21:37.43 Forearm.seek :retract @19:21:38.65 SE.stop @19:21:38.67 upsync @19:21:38.71
Starting finalPurge @19:21:41.15 delay 1 second @19:21:42.17 PRV.dial :waste2,avoiding: :ab2 @19:21:44.01 PTV.dial :PRVmixing @19:21:45.27 PSV.dial :mixloop @19:21:46.28 PS.seek :empty @19:21:58.40
Waiting >20 seconds for psFlush to relinquish FlushPuck @19:22:12.49 PSR.select :air @19:22:12.51 PSV.dial PSR @19:22:13.68 Pulling 0.5ml of PSR.flush @19:22:13.69 PSR.select :flush @19:22:13.81 PS.seek 0.5ml @19:22:19.65 delay 5 seconds @19:22:24.67 PSR.select :air @19:22:24.78 PS.seek 2.5ml @19:22:45.83 delay 1 second @19:22:46.85 PBV.dial :waste1 @19:22:47.21 PTV.dial :mixing @19:22:47.93 PSV.dial :mixloop @19:22:49.04 PS.seek :empty @19:23:15.31 PSV.dial PSR @19:23:16.28 PSR.select :flush @19:23:16.40 PS.seek 1ml @19:23:27.58 PSR.select :air @19:23:27.69 PS.seek 2.5ml @19:23:43.71 PSV.dial PTV @19:23:44.43 PTV.dial :puck @19:23:45.13 PBV.dial :waste1 @19:23:45.15 PS.seek 0ml @19:24:11.27 PSV.dial PSR @19:24:12.11 PSR.select :flush @19:24:12.23 PS.seek 0.5ml @19:24:18.17 PSR.select :air @19:24:18.29 PS.seek 2.5ml @19:24:39.36 PSV.dial PTV @19:24:40.07 PBV.dial PSV @19:24:40.62 PS.seek 0ml @19:25:06.83 PSV.dial PBV @19:25:07.29 PS.seek 2.5ml @19:25:33.52 PSV.dial :waste1 @19:25:34.24 PS.seek 0ml @19:26:00.45 PSV.dial PSR @19:26:00.91 PSR.select :air @19:26:00.93 PS.seek 2.5ml @19:26:27.05 PSV.dial PTV @19:26:27.77 PBV.dial PSV @19:26:27.79 PS.seek 0ml @19:26:54.01 PSV.dial PBV @19:26:54.47 PS.seek 2.5ml @19:27:20.86 PSV.dial :waste1 @19:27:21.58 PS.seek 0ml @19:27:47.73 PSV.dial PSR @19:27:48.18 PSR.select :flush @19:27:48.30 PS.seek 1ml @19:27:59.23 PSR.select :air @19:27:59.34 PS.seek 2.5ml @19:28:15.32 PSV.dial PTV @19:28:16.05 PTV.dial :puck @19:28:16.06 PBV.dial :waste1 @19:28:16.61 PS.seek 0ml @19:28:42.73 PSV.dial PSR @19:28:43.45 PSR.select :flush @19:28:43.56 PS.seek 0.5ml @19:28:49.45 PSR.select :air @19:28:49.56 PS.seek 2.5ml @19:29:10.67 PSV.dial PTV @19:29:11.39 PBV.dial PSV @19:29:11.94 PS.seek 0ml @19:29:38.10 PSV.dial PBV @19:29:38.56 PS.seek 2.5ml @19:30:04.95 PSV.dial :waste1 @19:30:05.78 PS.seek 0ml @19:30:32.01 PSV.dial PSR @19:30:32.55 PSR.select :air @19:30:32.58 PS.seek 2.5ml @19:30:58.85 PSV.dial PTV @19:30:59.59 PBV.dial PSV @19:30:59.61 PS.seek 0ml @19:31:25.72 PSV.dial PBV @19:31:26.18 PS.seek 2.5ml @19:31:52.32 PSV.dial :waste1 @19:31:53.04 PS.seek 0ml @19:32:19.20
Loading FlushPuck into Collection Clamp from Processing Clamp @19:32:19.37 Elbow.seek PC @19:32:21.93 Elbow.reconfigure :loose @19:32:22.02 Forearm.seek PC @19:32:22.96 Hand.close @19:32:23.54 PC.seek :open @19:32:27.49 Forearm.seek :retract @19:32:28.49 Elbow.reconfigure :tight @19:32:28.53 Elbow.seek CC @19:32:32.06 Elbow.reconfigure :loose @19:32:32.14 Forearm.reconfigure :tight @19:32:32.19 Forearm.seek CC @19:32:33.49 CC.seek :closed @19:32:37.72 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.83 @19:32:37.79 Hand.open @19:32:38.60 Forearm.reconfigure :loose @19:32:38.64 Forearm.seek :retract @19:32:39.71 Flushing the Collection Valve::Manifold @19:32:39.83 CSV.dial CSR @19:32:40.32 Pulling 4.999ml of CSR.flush @19:32:40.33 CSR.select :flush @19:32:40.45 CS.seek 5ml @19:32:53.83 delay 5 seconds @19:32:58.85 CSR.select :air @19:32:59.00 CS.reconfigure :fast @19:32:59.04 CS.seek 7.5ml @19:33:05.39 CSV.dial :waste @19:33:06.68 CS.reconfigure :default @19:33:06.72 CS.seek 0ml @19:33:26.39 Flushing lower manifold's waste1 port @19:33:26.51 CSV.dial :flush @19:33:27.07 CS.reconfigure :fast @19:33:27.11 CS.seek 5ml @19:33:38.98 delay 5 seconds @19:33:44.00 CSV.dial :air @19:33:44.51 CS.seek 7ml @19:33:49.74 CSV.dial CTV @19:33:49.76
CTV.dial CSV @19:33:51.08 CBV.dial :waste1 @19:33:51.82 CS.seek 0ml @19:34:08.14 Flushing lower manifold's waste2 port @19:34:08.26 CSV.dial :flush @19:34:08.99 CS.seek 5ml @19:34:20.89 delay 5 seconds @19:34:25.91 CSV.dial :air @19:34:26.42 CS.seek 7ml @19:34:31.74 CSV.dial CTV @19:34:31.77
CTV.dial CSV @19:34:32.19 CBV.dial :waste2 @19:34:34.18 CS.seek 0ml @19:34:50.36 Flushing lower manifold's waste2b port @19:34:50.48 CSV.dial :flush @19:34:51.21 CS.seek 5ml @19:35:03.01 delay 5 seconds @19:35:08.03 CSV.dial :air @19:35:08.54 CS.seek 7ml @19:35:13.83 CSV.dial CTV @19:35:13.85
CTV.dial CSV @19:35:14.28 CBV.dial :waste2b @19:35:15.29 CS.seek 0ml @19:35:31.55 Disinfecting external Intake with kill @19:35:31.66 CSV.dial CSR @19:35:32.15 Pulling 8ml of CSR.kill CSR.select :kill @19:35:32.27 CS.seek 8ml @19:35:50.73 delay 5 seconds @19:35:55.75 CSR.select :air @19:35:55.87 CS.seek 10ml @19:36:01.14 CSV.dial CBV @19:36:01.20
CBV.dial CSV @19:36:02.18 CTV.dial :intake @19:36:02.89 Intake.select :open @19:36:02.99 CS.seek 2ml @19:36:21.26 Intake.select :close @19:36:21.36 CTV.dial :air @19:36:22.11 CS.reconfigure :default @19:36:22.15 CS.seek 0ml @19:36:28.01 CSV.dial CSR @19:36:29.07 Pulling 4.999ml of CSR.flush CSR.select :flush @19:36:29.18 CS.seek 5ml @19:36:42.64 delay 5 seconds @19:36:47.65 CSR.select :air @19:36:47.77 CS.seek 10ml @19:37:01.20 CSV.dial CBV @19:37:02.19 CS.seek 0ml @19:37:28.20 Garaging FlushPuck in Collection Clamp @19:37:28.27 Elbow.reconfigure :tight @19:37:28.31 Elbow.seek CC @19:37:28.42 Elbow.reconfigure :loose @19:37:28.51 Forearm.seek CC @19:37:29.56 Hand.close @19:37:30.28 CC.seek :open @19:37:34.62 Forearm.seek :retract @19:37:35.78 Elbow.reconfigure :tight @19:37:35.82 Elbow.seek :garage @19:37:38.97 Elbow.reconfigure :loose @19:37:39.06 Forearm.reconfigure :tight @19:37:39.10 Forearm.seek :garage @19:37:40.22 Hand.open @19:37:41.41 Forearm.reconfigure :loose @19:37:41.46 Forearm.seek :retract @19:37:42.70 Completed [initialPurge; da [100, 100] #da and wsr are run simultanelously] begun 17:46:17 @19:37:42.75 Asleep after completing DA_WCR_10322 assays @19:37:42.77 GoodNews.email "{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:35:21, 23.6C, 21% humidity, 15.7psia, 13.826V, 0.386A, 0.386A avg, 5.33W\nSampled 100.0ml",Subject: "Asleep after completing DA_WCR_10322 assays" @19:38:16.81
Uploading Logs @19:38:19.86 upsync @17:12:34.42UTC06-Oct-22
>RESET< ESP2 v3.41 I2C Gateway v3.20 for core bus @17:12:37.02 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @17:12:40.95 Polling Can every 10:00 @17:12:40.98 Cleanly exited at 16:26:36UTC06-Oct-22 @17:12:41.02 Loading /home/esp/esp2/mission/DA_WCR_10622.rb mission @17:12:41.07 Preparing to sleep until + 00:00:05 @17:12:41.09 GoodNews.email "Starting DA_WCR_10622 w/pucks in tube 2\n{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:12:40, 24.9C, 12% humidity, 15.7psia, 14.050V, 0.086A, 0.086A avg, 1.20W",Subject: "DA_WCR_10622 begun" @17:12:41.13 Shutdown @17:12:41.17
Asleep til + 00:00:05 @17:12:41.18 Continuing at 17:12 @17:12:46.05 Powering core bus @17:12:51.42 Gate.power :raw,:ON @17:12:52.20 CS.configure :default @17:12:52.29 PS.configure :default @17:12:52.39 SS.configure :default @17:12:52.49 CC.configure :default @17:12:52.58 PC.configure :default @17:12:52.71 Forearm.configure :loose @17:12:52.79 Elbow.configure :loose @17:12:52.92 SC.configure :loose @17:12:53.01 SE.configure :default @17:12:53.07 CH.coast @17:12:53.13 CH.configure :default @17:12:53.16 PH.coast @17:12:53.21 PH.configure :default @17:12:53.24 All dwarf controllers running firmware version 3.85 @17:12:53.36 Valve.reset @17:12:53.60 Intake.select :close @17:12:53.64 Exhaust.select :close @17:12:53.65 CTV.dial :air @17:12:53.73 PSV.dial :air @17:12:53.83
Forearm.reconfigure :loose @17:12:53.88 Forearm.home.seek :retract @17:12:53.91 CSV.dial :waste1 @17:12:54.00 PS.reconfigure :default @17:12:54.05 PS.home.seek 0ml @17:12:54.40 SSV.dial :spit,avoiding: :exhaust @17:12:54.52 CS.reconfigure :fast @17:12:54.56 SS.reconfigure :default @17:12:54.63 CS.home.seek 0ml @17:12:55.73 SS.home.seek 0ml @17:12:55.78 Hand.close @17:12:55.80
Elbow.jog 1500 @17:12:56.30 Elbow.reconfigure :loose @17:12:56.34 Elbow.home.seek :clear @17:12:59.48 SE.reconfigure :default @17:12:59.52 SE.home.seek :down @17:13:00.12 SC.reconfigure :tight @17:13:00.17 SC.home.seek :initial @17:13:02.48 Net::SMTPUnknownError in Qmail -- could not get 3xx (421) @17:13:02.51 Email::Queue::Purge in Qmail -- Purged output queue @17:13:02.70 PS.stop @17:13:30.21 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @17:13:30.22
Retry #1 of 3 @17:13:30.29 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @17:13:31.50 SC.configure :limp @17:13:31.55 Forearm.seek :retract @17:13:31.61 Elbow.seek :hammer @17:13:32.69 Forearm.reconfigure :tight @17:13:32.73 Forearm.seek retracted @17:13:33.11 PC.reconfigure :default @17:13:33.14
CC.reconfigure :default @17:13:33.21 PC.home.seek :open @17:13:33.27
CC.home.seek :open @17:13:36.39 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @17:13:36.41 Notice.email "At 17:13:36 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_10622.rb:14\n{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:13:36, 24.9C, 12% humidity, 15.7psia, 13.967V, 0.404A, 0.398A avg, 5.64W",Subject: "Started DA_WCR_10622 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @17:13:36.43
Starting initialPurge @17:13:36.45 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:13:36.51 Hand.open @17:13:37.39 Elbow.reconfigure :tight @17:13:37.43 Elbow.seek FlushPuck::Garage @17:13:39.40 Elbow.reconfigure :loose @17:13:39.49 Forearm.reconfigure :loose @17:13:39.53 Forearm.seek :garage @17:13:40.62 Hand.close @17:13:41.38 Forearm.seek :retract @17:13:42.42 Elbow.reconfigure :tight @17:13:42.46 Elbow.seek CC @17:13:45.32 Elbow.reconfigure :loose @17:13:45.41 Forearm.reconfigure :tight @17:13:45.45 Forearm.seek CC @17:13:46.69 CC.seek :closed @17:13:50.92 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.86 @17:13:50.99 Hand.open @17:13:51.83 Forearm.reconfigure :loose @17:13:51.87 Forearm.seek :retract @17:13:52.95 Flushing the Collection Valve::Manifold @17:13:53.07 CSV.dial CSR @17:13:54.38 Pulling 5ml of CSR.flush @17:13:54.39 CSR.select :flush @17:13:54.51 CS.reconfigure :default @17:13:54.55 CS.seek 5ml @17:14:07.96 delay 5 seconds @17:14:12.97 CSR.select :air @17:14:13.12 CS.reconfigure :fast @17:14:13.17 CS.seek 7.5ml @17:14:19.45 CSV.dial :waste @17:14:20.71 CS.reconfigure :default @17:14:20.75 CS.seek 0ml @17:14:40.42 Purging external Intake @17:14:40.54 CSV.dial :flush @17:14:40.97 CS.reconfigure :fast @17:14:41.02 CS.seek 10ml @17:15:03.61 delay 5 seconds @17:15:08.63 CSV.dial CBV @17:15:08.66
CBV.dial CSV @17:15:09.40 CTV.dial :intake @17:15:10.11 Intake.select :open @17:15:10.21 CS.seek 0ml @17:15:32.79 Intake.select :close @17:15:32.89 CTV.dial :air @17:15:33.64 Flushing upper manifold @17:15:33.76 CSV.dial :flush @17:15:34.49 CS.seek 5ml @17:15:46.19 delay 5 seconds @17:15:51.22 CSV.dial :air @17:15:51.81 CS.seek 7ml @17:15:57.17 CSV.dial CBV @17:15:57.21
CBV.dial CSV @17:15:58.28 CTV.dial :air @17:15:58.32 CS.seek 0ml @17:16:13.99 upsync @17:16:14.38
delay 7 seconds @17:16:21.40 CS.seek 3ml @17:16:28.85 CSV.dial :waste @17:16:29.32 CS.seek 0ml @17:16:36.80 CSV.dial :flush @17:16:37.36 CS.seek 3ml @17:16:44.67 delay 5 seconds @17:16:49.68 CSV.dial :air @17:16:50.20 CS.seek 5ml @17:16:55.39 CSV.dial :air @17:16:55.40 CS.seek 0ml @17:17:07.01 delay 7 seconds @17:17:14.04 CS.seek 3ml @17:17:21.48 CSV.dial :waste @17:17:22.19 CS.seek 0ml @17:17:29.54 Garaging FlushPuck in Collection Clamp @17:17:29.61 Elbow.reconfigure :tight @17:17:29.65 Elbow.seek CC @17:17:29.84 Elbow.reconfigure :loose @17:17:29.93 Forearm.seek CC @17:17:30.91 Hand.close @17:17:31.63 CC.seek :open @17:17:35.96 Forearm.seek :retract @17:17:36.93 Elbow.reconfigure :tight @17:17:36.97 Elbow.seek :garage @17:17:39.84 Elbow.reconfigure :loose @17:17:39.93 Forearm.reconfigure :tight @17:17:39.97 Forearm.seek :garage @17:17:41.12 Hand.open @17:17:42.40 Forearm.reconfigure :loose @17:17:42.44 Forearm.seek :retract @17:17:43.57 da sampling at most 1000ml, wcr at most 1000ml @17:17:43.60 DAfans.email "Started da in tube 2 at 17:17:43UTC06-Oct-22\n{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:13:36, 24.9C, 12% humidity, 15.7psia, 13.967V, 0.404A, 0.398A avg, 5.64W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @17:17:43.64
Loading sh1 puck from tube 2 into Collection Clamp @17:17:43.87 SC.reconfigure :tight @17:17:43.91 SC.seek 2 @17:17:56.86 SC.configure :limp @17:17:57.06 Elbow.reconfigure :tight @17:17:57.10 Elbow.seek 2 @17:17:59.12 Forearm.reconfigure :tight @17:17:59.16 Forearm.seek 2 + 700 counts @17:18:00.20 SE.reconfigure :pick @17:18:00.25 SE.seek :beamBreak @17:18:01.55 SE.configure :default @17:18:01.69 Elbow.seek 2 @17:18:02.10 Forearm.seek 2 + 300 counts @17:18:02.36 Forearm.reconfigure :loose @17:18:02.40 Hand.close @17:18:03.02 SE.seek :down @17:18:03.22
Forearm.seek :retract @17:18:03.91 SE.stop @17:18:04.26
Elbow.seek CC @17:18:05.93 Elbow.reconfigure :loose @17:18:06.03 Forearm.reconfigure :tight @17:18:06.07 Forearm.seek CC @17:18:07.35 CC.seek :closed @17:18:11.55 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.72 @17:18:11.56 Hand.open @17:18:12.31 Forearm.reconfigure :loose @17:18:12.35 Forearm.seek :retract @17:18:13.35 collectSample 1000ml w/bubble point of 24psi @17:18:13.36 Priming 7ml @17:18:13.37 Intake.select :close @17:18:13.38 Exhaust.select :close @17:18:13.39 SS.hold @17:18:13.59 Calibrating pressure and temperature sensors @17:18:13.62 CTV.dial :air @17:18:13.63 SSV.dial :spit,avoiding: :exhaust @17:18:13.65 SS.reconfigure :fast @17:18:13.70 SS.seek 5ml @17:18:16.59 SSV.dial CBV @17:18:16.63
CBV.dial SSV @17:18:17.90 delay 3.8 seconds @17:18:22.00 SSV.dial :spit @17:18:23.32 SS.seek 0ml @17:18:26.13 SSV.dial CBV @17:18:27.49 CTV.dial :intake @17:18:28.37 SSV.dial CBV @17:18:28.43
CBV.dial SSV @17:18:28.46 Allowing 40s for 7.0ml of remaining 7.0ml @17:18:28.49 Intake.select :open @17:18:30.80 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:18:30.84 SS.reconfigure :inhaling 2.14ml/s @17:18:30.92 SS.seek 7ml @17:18:37.96 Stroke completed -- waiting for pressure to equalize ... @17:18:37.98 SS.reconfigure :exhale @17:18:38.06 SS.hold @17:18:38.51 Intake.select :close @17:18:38.76 SSV.dial :exhaust @17:18:39.52 Exhaust.select :open @17:18:39.67 SS.seek 0ml @17:18:43.63 Exhaust.select :close @17:18:43.83 Sampling 1000.0ml @17:18:43.85 Intake.select :close @17:18:43.87 Exhaust.select :close @17:18:43.89 SS.reconfigure :default @17:18:43.97 SS.hold @17:18:44.36 CTV.dial :intake @17:18:44.40 SSV.dial CBV @17:18:44.44
CBV.dial SSV @17:18:45.24 Allowing 130s for 25.0ml of remaining 1000.0ml @17:18:45.26 Intake.select :open @17:18:47.51 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:18:47.54 SS.reconfigure :inhaling 2.14ml/s @17:18:47.65 SS.seek 25ml @17:18:55.37 upsync @17:18:55.53
Vacuum too high -- waiting up to 40 seconds @17:18:55.54 SS.reconfigure :exhale @17:18:55.58 SS.hold @17:18:56.26 Intake @10.7psia, vacuum from 5.2 to 8.2psi @17:18:56.28 SS.reconfigure :inhaling 1.93ml/s @17:18:56.32 SS.seek 25ml @17:19:01.03 Vacuum too high -- waiting up to 40 seconds @17:19:01.04 SS.reconfigure :exhale @17:19:01.08 SS.hold @17:19:03.10 Intake @13.4psia, vacuum from 7.9 to 10.9psi @17:19:03.11 SS.reconfigure :inhaling 1.74ml/s @17:19:03.16 SS.seek 25ml @17:19:09.31 Stroke completed -- waiting for pressure to equalize ... @17:19:09.32 SS.reconfigure :exhale @17:19:09.37 SS.hold @17:19:10.32 Intake.select :close @17:19:10.46 SSV.dial :exhaust @17:19:11.19 Exhaust.select :open @17:19:11.30 SS.seek 0ml @17:19:20.09 Exhaust.select :close @17:19:20.20 SSV.dial CBV @17:19:20.22
CBV.dial SSV @17:19:20.96 Allowing 130s for 25.0ml of remaining 975.0ml @17:19:20.97 Intake.select :open @17:19:23.12 Intake @15.0psia, vacuum from 9.5 to 12.5psi @17:19:23.13 SS.reconfigure :inhaling 1.74ml/s @17:19:23.17 SS.seek 25ml @17:19:32.87 Vacuum too high -- waiting up to 40 seconds @17:19:32.88 SS.reconfigure :exhale @17:19:32.92 SS.hold @17:19:33.54 Intake @10.3psia, vacuum from 4.8 to 7.8psi @17:19:33.56 SS.reconfigure :inhaling 1.57ml/s @17:19:33.62 SS.seek 25ml @17:19:43.44 Stroke completed -- waiting for pressure to equalize ... @17:19:43.45 SS.reconfigure :exhale @17:19:43.49 SS.hold @17:19:44.76 Intake.select :close @17:19:44.90 SSV.dial :exhaust @17:19:45.63 Exhaust.select :open @17:19:45.73 SS.seek 0ml @17:19:54.92 Exhaust.select :close @17:19:55.02 SSV.dial CBV @17:19:55.06
CBV.dial SSV @17:19:55.78 Allowing 130s for 25.0ml of remaining 950.0ml @17:19:55.79 Intake.select :open @17:19:57.94 Intake @14.9psia, vacuum from 9.4 to 12.4psi @17:19:57.96 SS.reconfigure :inhaling 1.57ml/s @17:19:58.00 SS.seek 25ml @17:20:04.80 Vacuum too high -- waiting up to 40 seconds @17:20:04.81 SS.reconfigure :exhale @17:20:04.86 SS.hold @17:20:05.40 Intake @12.0psia, vacuum from 6.5 to 9.5psi @17:20:05.42 SS.reconfigure :inhaling 1.41ml/s @17:20:05.46 SS.seek 25ml @17:20:10.50 Vacuum too high -- waiting up to 40 seconds @17:20:10.51 SS.reconfigure :exhale @17:20:10.55 SS.hold @17:20:12.29 Intake @13.4psia, vacuum from 7.9 to 10.9psi @17:20:12.31 SS.reconfigure :inhaling 1.27ml/s @17:20:12.35 SS.seek 25ml @17:20:17.62 Vacuum too high -- waiting up to 40 seconds @17:20:17.63 SS.reconfigure :exhale @17:20:17.67 SS.hold @17:20:19.79 Intake @13.7psia, vacuum from 8.2 to 11.2psi @17:20:19.80 SS.reconfigure :inhaling 1.14ml/s @17:20:19.85 SS.seek 25ml @17:20:25.31 Stroke completed -- waiting for pressure to equalize ... @17:20:25.32 SS.reconfigure :exhale @17:20:25.36 SS.hold @17:20:28.26 Intake.select :close @17:20:28.40 SSV.dial :exhaust @17:20:29.12 Exhaust.select :open @17:20:29.23 SS.seek 0ml @17:20:38.07 Exhaust.select :close @17:20:38.18 SSV.dial CBV @17:20:38.20
CBV.dial SSV @17:20:38.95 Allowing 130s for 25.0ml of remaining 925.0ml @17:20:38.96 Intake.select :open @17:20:41.11 Intake @14.9psia, vacuum from 9.4 to 12.4psi @17:20:41.13 SS.reconfigure :inhaling 1.14ml/s @17:20:41.17 SS.seek 25ml @17:20:46.14 Vacuum too high -- waiting up to 40 seconds @17:20:46.15 SS.reconfigure :exhale @17:20:46.20 SS.hold @17:20:48.31 Intake @14.4psia, vacuum from 8.9 to 11.9psi @17:20:48.33 SS.reconfigure :inhaling 1.03ml/s @17:20:48.37 SS.seek 25ml @17:20:52.11 Vacuum too high -- waiting up to 40 seconds @17:20:52.12 SS.reconfigure :exhale @17:20:52.16 SS.hold @17:20:55.48 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:20:55.49 SS.reconfigure :inhaling 0.93ml/s @17:20:55.53 SS.seek 25ml @17:20:58.99 Vacuum too high -- waiting up to 40 seconds @17:20:59.00 SS.reconfigure :exhale @17:20:59.04 SS.hold @17:21:03.20 Intake @14.9psia, vacuum from 9.4 to 12.4psi @17:21:03.22 SS.reconfigure :inhaling 0.84ml/s @17:21:03.26 SS.seek 25ml @17:21:06.89 Vacuum too high -- waiting up to 40 seconds @17:21:06.90 SS.reconfigure :exhale @17:21:06.95 SS.hold @17:21:15.77 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:21:15.79 SS.reconfigure :inhaling 0.76ml/s @17:21:15.83 SS.seek 25ml @17:21:18.58 Vacuum too high -- waiting up to 40 seconds @17:21:18.59 SS.reconfigure :exhale @17:21:18.63 SS.hold @17:21:58.87 SS.reconfigure :default @17:21:58.91 SS.hold @17:21:59.02 Intake.select :close @17:21:59.16 ShallowSampler::Clogged in MAIN -- Intake @14.9psia, & Outlet @3.7psia -- no progress after 86.9ml @17:21:59.17 SSV.dial :exhaust @17:21:59.90 Exhaust.select :open @17:22:00.01 SS.reconfigure :exhale @17:22:00.05 SS.seek 0ml @17:22:05.87 Exhaust.select :close @17:22:05.97 Sampled 86.9ml @17:22:05.98 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:22:05.99 Intake.select :close @17:22:06.05 CSV.dial :waste @17:22:06.09 CS.seek 0ml @17:22:06.19 SSV.dial CBV,avoiding: :exhaust @17:22:06.21
CBV.dial SSV @17:22:06.27 CSV.dial :air @17:22:06.99 CS.seek 10ml @17:22:08.03
CTV.dial :air @17:22:08.75 delay 15 seconds @17:22:23.77 SSV.dial :spit,avoiding: :exhaust @17:22:25.06 CTV.dial CSV,avoiding: :intake @17:22:25.10
SS.seek 0ml @17:22:25.20 SSV.dial CBV,avoiding: :exhaust @17:22:29.90 CBV.dial between SSV and waste2 @17:22:30.27 CTV.dial CSV,avoiding: :air @17:22:30.30
CSV.dial CTV @17:22:30.73 CS.reconfigure :pressurize @17:22:30.78 CS.seek 0ml @17:22:50.00 BubblePoint: Intake @32.5psia, Outlet @8.2psia @17:22:50.01 CBV.dial SSV @17:22:50.32 SS.reconfigure :default @17:22:50.36 SS.seek 10ml @17:22:56.21 delay 15 seconds @17:23:11.33 Evacuated: Intake @16.6psia, Outlet @9.7psia @17:23:11.34 SSV.dial :spit,avoiding: :exhaust @17:23:12.64 SS.reconfigure :exhale @17:23:12.68 SS.seek 0ml @17:23:17.80 SS.reconfigure :fast @17:23:17.84 SS.seek 5ml @17:23:20.64 SSV.dial CBV,avoiding: :exhaust @17:23:20.66
CBV.dial SSV @17:23:21.95 CTV.dial :air,avoiding: :intake @17:23:23.21 CSV.dial CTV @17:23:23.24
SS.seek 10ml @17:23:24.24 CSV.dial :air @17:23:24.69 CS.reconfigure :fast @17:23:24.74 CS.seek 0ml @17:23:25.74
SSV.dial :spit,avoiding: :exhaust @17:23:27.05 SS.reconfigure :exhale @17:23:27.09 SS.seek 0ml @17:23:31.12 CS.stop @17:23:32.33
SS.reconfigure :fast @17:23:32.37 SS.seek 5ml @17:23:35.23 SSV.dial CBV,avoiding: :exhaust @17:23:35.26
CBV.dial SSV @17:23:37.55 SSV.dial :spit,avoiding: :exhaust @17:23:38.86 SS.reconfigure :default @17:23:38.90 SS.seek 0ml @17:23:42.16 SS.stop @17:23:42.20 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:23:42.21 Intake.select :close @17:23:42.27 CSV.dial :waste @17:23:42.39 SSV.dial CBV,avoiding: :exhaust @17:23:42.41
CBV.dial SSV @17:23:43.16 CS.seek 0ml @17:23:43.22 CSV.dial :air @17:23:43.70
CTV.dial :air @17:23:43.71 delay 15 seconds @17:23:43.93 CS.seek 10ml @17:23:58.73
SSV.dial :spit,avoiding: :exhaust @17:24:00.03 CTV.dial CSV,avoiding: :intake @17:24:00.45
SS.reconfigure :exhale @17:24:00.63 SS.seek 0ml @17:24:00.70 SSV.dial CBV,avoiding: :exhaust @17:24:06.62 CBV.dial between SSV and waste2 @17:24:07.00 CTV.dial CSV,avoiding: :air @17:24:07.03
CSV.dial CTV @17:24:07.47 CS.reconfigure :pressurize @17:24:07.52 CS.seek 0ml @17:24:29.53 BubblePoint: Intake @39.2psia, Outlet @15.2psia @17:24:29.54 CBV.dial SSV @17:24:29.86 SS.reconfigure :default @17:24:29.91 SS.seek 10ml @17:24:35.48 delay 15 seconds @17:24:50.59 Evacuated: Intake @17.4psia, Outlet @11.0psia @17:24:50.60 SSV.dial :spit,avoiding: :exhaust @17:24:51.90 SS.reconfigure :exhale @17:24:51.94 SS.seek 0ml @17:24:57.55 SS.reconfigure :fast @17:24:57.59 SS.seek 5ml @17:25:00.23 SSV.dial CBV,avoiding: :exhaust @17:25:00.25
CBV.dial SSV @17:25:01.54 CTV.dial :air,avoiding: :intake @17:25:02.81 CSV.dial CTV @17:25:02.84
SS.seek 10ml @17:25:03.83 CSV.dial :air @17:25:04.28 CS.reconfigure :fast @17:25:04.32 CS.seek 0ml @17:25:05.32
SSV.dial :spit,avoiding: :exhaust @17:25:06.62 SS.reconfigure :exhale @17:25:06.66 SS.seek 0ml @17:25:08.43 CS.stop @17:25:12.17
SS.reconfigure :fast @17:25:12.21 SS.seek 5ml @17:25:15.01 SSV.dial CBV,avoiding: :exhaust @17:25:15.03
CBV.dial SSV @17:25:17.31 SSV.dial :spit,avoiding: :exhaust @17:25:18.59 SS.reconfigure :default @17:25:18.63 SS.seek 0ml @17:25:21.72 SS.stop @17:25:21.76 Lysing: Adding 2.0ml AqMeOH @17:25:21.77 CBV.dial between CSV and waste1,avoiding: :DBV @17:25:23.20 CTV.dial CSV @17:25:25.09 CSV.dial CSR @17:25:25.86 Pulling 1ml of CSR.AqMeOH @17:25:25.87 CSR.select :AqMeOH @17:25:25.98 CS.reconfigure :default @17:25:26.02 CS.seek 1ml @17:25:29.37 delay 5 seconds @17:25:34.39 CSR.select :air @17:25:34.53 CS.seek 6ml @17:25:47.90 delay 2 seconds @17:25:49.91 CSV.dial :waste2 @17:25:50.34 CS.reconfigure :fast @17:25:50.39 CS.seek 0ml @17:26:04.30 CSV.dial CSR @17:26:04.91 Pulling 2ml of CSR.AqMeOH @17:26:04.92 CSR.select :AqMeOH @17:26:05.02 CS.reconfigure :default @17:26:05.06 CS.seek 2ml @17:26:10.90 delay 5 seconds @17:26:15.92 CSR.select :air @17:26:16.08 CS.seek 4.999ml @17:26:24.55 delay 2 seconds @17:26:26.57 CSV.dial CTV @17:26:27.02 CS.seek 1ml @17:26:37.97 Before lysing Collection Puck Heater at 19C/38.1psia @17:26:37.99 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @17:26:38.01 CH.seek 70C,8:00 @17:28:33.71 CH.hold 10:00,70C @17:28:33.88 Begun lysing Collection Puck Heater at 68C/35.0psia @17:38:33.89 Ended lysing Collection Puck Heater at 70C/28.5psia @17:38:33.91 CS.seek 0ml @17:38:37.25 CTV.dial between spare and air,avoiding: :intake @17:38:38.22 CSV.dial :air @17:38:38.67 CS.seek 5ml @17:38:52.13 CSV.dial CTV @17:38:52.57 delay 2 seconds @17:38:54.58 CSV.dial :air @17:38:55.02 delay 2 seconds @17:38:57.03 CSV.dial CTV @17:38:57.60 delay 2 seconds @17:38:59.62 CSV.dial :air @17:39:00.05 delay 2 seconds @17:39:02.07 CS.seek 0ml @17:39:15.55 Puck @25.7psia after final lysis pressurization @17:39:15.57 Recovering Lysate into Collection syringe CSV.dial CBV @17:39:16.56 CBV.dial CSV @17:39:16.92 CS.seek 5ml @17:39:30.31 delay 5 seconds @17:39:35.33 CTV.dial :air @17:39:35.83 CS.seek 7ml @17:39:41.63 delay 5 seconds @17:39:46.64 CSV.dial :air2 @17:39:47.09 CS.seek 10ml @17:39:55.44 CSV.dial between air and CTV,avoiding: CBV @17:39:56.57 Lysing Complete @17:39:56.64 Storing sh1 puck in Collection Clamp to tube 1 @17:39:56.80 SC.reconfigure :tight @17:39:56.84 SC.seek 1 @17:40:00.45 SC.configure :limp @17:40:00.65 Elbow.reconfigure :tight @17:40:00.69 Elbow.seek 1 @17:40:02.46 Forearm.reconfigure :tight @17:40:02.50 Forearm.seek 1 + 700 counts @17:40:03.48 SE.reconfigure :pick @17:40:03.53 SE.seek :beamBreak @17:40:06.43 SE.configure :default @17:40:06.57 Elbow.reconfigure :limp @17:40:06.62 Forearm.reconfigure :loose @17:40:06.66 Forearm.seek 1 @17:40:07.06 Elbow.hold @17:40:07.12 Elbow.reconfigure :tight @17:40:07.21 SE.seek 3.22 Pucks below Top Plate @17:40:07.63 Forearm.force -20 @17:40:07.67 Forearm.seek 1 + 319 counts @17:40:07.96 Forearm.reconfigure :tight @17:40:08.00 Forearm.seek 1 + 700 counts @17:40:08.29 SE.seek 4.22 Pucks below Top Plate @17:40:08.77 Forearm.reconfigure :loose @17:40:08.81 Forearm.seek :retract @17:40:09.80 Elbow.seek CC @17:40:11.48 Elbow.reconfigure :loose @17:40:11.57 Forearm.seek CC @17:40:12.53 Hand.close @17:40:13.21 CC.seek :open @17:40:17.65 Forearm.seek :retract @17:40:18.66 Elbow.reconfigure :tight @17:40:18.70 Elbow.seek 1 @17:40:20.47 Elbow.reconfigure :limp @17:40:20.51 Forearm.seek 1 @17:40:21.63 Elbow.hold @17:40:21.69 Elbow.reconfigure :tight @17:40:21.73 Hand.open @17:40:22.57 Forearm.force -20 @17:40:22.61 Forearm.seek 1 + 314 counts @17:40:22.87 Forearm.reconfigure :tight @17:40:22.91 Forearm.seek 1 + 700 counts @17:40:23.22 SE.seek :down @17:40:24.34
Forearm.reconfigure :loose @17:40:24.38 Forearm.seek :retract @17:40:25.74 SE.stop @17:40:25.78
Tranferring DA @17:40:25.79 Beginning samplePassC @17:40:25.81 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:40:26.45 Elbow.seek FlushPuck::Garage @17:40:28.18 Elbow.reconfigure :loose @17:40:28.27 Forearm.seek :garage @17:40:29.38 Hand.close @17:40:30.07 Forearm.seek :retract @17:40:31.13 Elbow.reconfigure :tight @17:40:31.17 Elbow.seek CC @17:40:34.04 Elbow.reconfigure :loose @17:40:34.13 Forearm.reconfigure :tight @17:40:34.17 Forearm.seek CC @17:40:35.43 CC.seek :closed @17:40:39.66 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.94 @17:40:39.72 Hand.open @17:40:40.51 Forearm.reconfigure :loose @17:40:40.55 Forearm.seek :retract @17:40:41.67 PSV.dial PSR @17:40:43.00 Pulling 1.5ml of PSR.flush @17:40:43.01 PSR.select :flush @17:40:43.13 PS.seek 1.5ml @17:40:59.12 delay 5 seconds @17:41:04.14 PSV.dial :waste1 @17:41:04.61 PS.seek 0ml @17:41:20.74 PSV.dial PSR @17:41:21.29 PSR.select :air @17:41:21.40 PS.seek 2.5ml @17:41:47.52 PRV.dial CBV,avoiding: :ab2 @17:41:47.55
CBV.dial PRV @17:41:49.19 PSV.dial :mixloop @17:41:50.19 CTV.dial :air,avoiding: :intake @17:41:50.21 PTV.dial :PRVmixing @17:41:51.43 PS.seek 0ml @17:42:17.61 PSV.dial :air @17:42:18.04 PS.seek 2.5ml @17:42:44.27 PSV.dial :mixloop @17:42:44.71 PS.seek 0ml @17:43:10.86 PSV.dial :air @17:43:11.30 PS.seek 2.5ml @17:43:37.58 PSV.dial :mixloop @17:43:38.02 PS.seek 0ml @17:44:04.24 Garaging FlushPuck in Collection Clamp @17:44:04.30 Elbow.reconfigure :tight @17:44:04.35 Elbow.seek CC @17:44:04.45 Elbow.reconfigure :loose @17:44:04.54 Forearm.seek CC @17:44:05.52 Hand.close @17:44:06.20 CC.seek :open @17:44:10.54 Forearm.seek :retract @17:44:11.54 Elbow.reconfigure :tight @17:44:11.58 Elbow.seek :garage @17:44:14.70 Elbow.reconfigure :loose @17:44:14.79 Forearm.reconfigure :tight @17:44:14.83 Forearm.seek :garage @17:44:15.91 Hand.open @17:44:16.75 Forearm.reconfigure :loose @17:44:16.80 Forearm.seek :retract @17:44:17.90 PSV.dial :mixloop @17:44:17.91 PTV.dial :PRVmixing @17:44:17.92 PRV.dial CBV @17:44:17.95
CBV.dial PRV @17:44:17.96 CTV.dial :air,avoiding: :intake @17:44:17.98 Loading lyfil puck from tube 2 into Collection Clamp @17:44:18.20 SC.reconfigure :tight @17:44:18.24 SC.seek 2 @17:44:21.93 SC.configure :limp @17:44:22.12 Elbow.reconfigure :tight @17:44:22.16 Elbow.seek 2 @17:44:24.55 Forearm.reconfigure :tight @17:44:24.59 Forearm.seek 2 + 700 counts @17:44:25.65 SE.reconfigure :pick @17:44:25.70 SE.seek :beamBreak @17:44:27.02 SE.configure :default @17:44:27.16 Elbow.seek 2 @17:44:27.34 Forearm.seek 2 + 300 counts @17:44:27.60 Forearm.reconfigure :loose @17:44:27.64 Hand.close @17:44:28.21 SE.seek :down @17:44:28.41
Forearm.seek :retract @17:44:29.16 SE.stop @17:44:29.37
Elbow.seek CC @17:44:31.02 Elbow.reconfigure :loose @17:44:31.11 Forearm.reconfigure :tight @17:44:31.15 Forearm.seek CC @17:44:32.47 CC.seek :closed @17:44:36.70 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.65 @17:44:36.71 Hand.open @17:44:37.51 Forearm.reconfigure :loose @17:44:37.55 Forearm.seek :retract @17:44:38.67 CTV.dial CSV,avoiding: :intake @17:44:39.93 CSV.dial CTV @17:44:40.23 PS.seek 2.5ml @17:45:06.42 CS.seek 5.499ml @17:45:18.62 delay 8 seconds @17:45:26.64 PSV.dial :air @17:45:27.09 delay 2 seconds @17:45:29.11 PSV.dial :waste2 @17:45:29.83 PS.seek 0ml @17:45:56.01 CBV.dial between PRV and waste1 @17:45:56.34 CSV.dial :air @17:45:56.79 CS.seek 10ml @17:46:09.02 CSV.dial CTV @17:46:09.47 CS.seek 0ml @17:46:35.39 CBV.dial :waste1 @17:46:35.69 delay 5 seconds @17:46:40.71 CTV.dial :air,avoiding: :intake @17:46:42.03 Storing lyfil puck in Collection Clamp to tube 1 @17:46:42.19 SC.reconfigure :tight @17:46:42.23 SC.seek 1 @17:46:45.84 SC.configure :limp @17:46:46.04 Elbow.reconfigure :tight @17:46:46.08 Elbow.seek 1 @17:46:47.95 Forearm.reconfigure :tight @17:46:47.99 Forearm.seek 1 + 700 counts @17:46:49.10 SE.reconfigure :pick @17:46:49.15 SE.seek :beamBreak @17:46:51.99 SE.configure :default @17:46:52.13 Elbow.reconfigure :limp @17:46:52.18 Forearm.reconfigure :loose @17:46:52.22 Forearm.seek 1 @17:46:52.61 Elbow.hold @17:46:52.68 Elbow.reconfigure :tight @17:46:52.77 SE.seek 4.23 Pucks below Top Plate @17:46:53.20 Forearm.force -20 @17:46:53.24 Forearm.seek 1 + 320 counts @17:46:53.57 Forearm.reconfigure :tight @17:46:53.61 Forearm.seek 1 + 700 counts @17:46:53.92 SE.seek 5.23 Pucks below Top Plate @17:46:54.51 Forearm.reconfigure :loose @17:46:54.55 Forearm.seek :retract @17:46:55.96 Elbow.seek CC @17:46:57.67 Elbow.reconfigure :loose @17:46:57.77 Forearm.seek CC @17:46:58.71 Hand.close @17:46:59.44 CC.seek :open @17:47:03.90 Forearm.seek :retract @17:47:04.90 Elbow.reconfigure :tight @17:47:04.94 Elbow.seek 1 @17:47:06.72 Elbow.reconfigure :limp @17:47:06.77 Forearm.seek 1 @17:47:07.91 Elbow.hold @17:47:07.97 Elbow.reconfigure :tight @17:47:08.01 Hand.open @17:47:08.81 Forearm.force -20 @17:47:08.85 Forearm.seek 1 + 319 counts @17:47:09.10 Forearm.reconfigure :tight @17:47:09.15 Forearm.seek 1 + 700 counts @17:47:09.55 SE.seek :down @17:47:10.68
Forearm.reconfigure :loose @17:47:10.72 Forearm.seek :retract @17:47:11.90 SE.stop @17:47:11.94
PSV.dial :air @17:47:12.66 PS.seek 2.5ml @17:47:38.79 delay 1 second @17:47:39.80 PSV.dial :mixloop @17:47:40.23 delay 1 second @17:47:41.24 PSV.dial :air @17:47:41.68 delay 1 second @17:47:42.70 PSV.dial :mixloop @17:47:43.14 delay 1 second @17:47:44.15 PSV.dial :air @17:47:44.60 delay 1 second @17:47:45.62 PS.seek 0ml @17:48:11.79 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:48:11.96 Elbow.seek FlushPuck::Garage @17:48:13.79 Elbow.reconfigure :loose @17:48:13.88 Forearm.seek :garage @17:48:14.95 Hand.close @17:48:15.68 Forearm.seek :retract @17:48:16.74 Elbow.reconfigure :tight @17:48:16.79 Elbow.seek PC @17:48:18.07 Elbow.reconfigure :loose @17:48:18.16 Forearm.reconfigure :tight @17:48:18.20 Forearm.seek PC @17:48:19.54 PC.seek :closed @17:48:23.41 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.45 @17:48:23.48 Hand.open @17:48:24.24 Forearm.reconfigure :loose @17:48:24.28 Forearm.seek :retract @17:48:25.41 PRV.dial :air @17:48:25.85 PSV.dial :mixloop @17:48:26.37 PS.seek 0.3ml @17:48:30.22 PSV.dial PSR @17:48:31.25 Pulling 0.4ml of PSR.air @17:48:31.26 PSR.select :air @17:48:31.28 PS.seek 0.7ml @17:48:36.19 delay 3 seconds @17:48:39.21 PSR.select :air @17:48:39.23 PS.seek 2.5ml @17:48:58.38 delay 3 seconds @17:49:01.40 PSV.dial :mixloop @17:49:02.38 PTV.dial :mixing @17:49:03.10 PBV.dial :waste2 @17:49:03.98 PS.seek 0ml @17:49:30.10 Garaging FlushPuck in Processing Clamp @17:49:30.17 Elbow.reconfigure :tight @17:49:30.21 Elbow.seek PC @17:49:30.32 Elbow.reconfigure :loose @17:49:30.87 Forearm.seek PC @17:49:31.79 Hand.close @17:49:32.44 PC.seek :open @17:49:36.40 Forearm.seek :retract @17:49:37.38 Elbow.reconfigure :tight @17:49:37.42 Elbow.seek :garage @17:49:38.80 Elbow.reconfigure :loose @17:49:38.89 Forearm.reconfigure :tight @17:49:38.94 Forearm.seek :garage @17:49:40.09 Hand.open @17:49:40.87 Forearm.reconfigure :loose @17:49:40.91 Forearm.seek :retract @17:49:41.94 Loading da puck from tube 2 into Processing Clamp @17:49:42.16 SC.reconfigure :tight @17:49:42.20 SC.seek 2 @17:49:45.78 SC.configure :limp @17:49:45.97 Elbow.reconfigure :tight @17:49:46.02 Elbow.seek 2 @17:49:47.91 Forearm.reconfigure :tight @17:49:47.95 Forearm.seek 2 + 700 counts @17:49:49.00 SE.reconfigure :pick @17:49:49.05 SE.seek :beamBreak @17:49:50.42 SE.configure :default @17:49:50.58 Elbow.seek 2 @17:49:50.75 Forearm.seek 2 + 300 counts @17:49:50.98 Forearm.reconfigure :loose @17:49:51.02 Hand.close @17:49:51.70 SE.seek :down @17:49:51.89
Forearm.seek :retract @17:49:52.75 SE.stop @17:49:52.88
Elbow.seek PC @17:49:55.43 Elbow.reconfigure :loose @17:49:55.52 Forearm.reconfigure :tight @17:49:55.56 Forearm.seek PC @17:49:56.97 PC.seek :closed @17:50:00.86 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.39 @17:50:00.87 Hand.open @17:50:01.62 Forearm.reconfigure :loose @17:50:01.66 Forearm.seek :retract @17:50:02.74 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @17:50:02.76 PH.seek 28C,5:00 @17:50:50.96 PH.hold 2:00:00,28C @17:50:51.00 delay 1:30 @17:52:21.04 PSR.select :air @17:52:21.05 PSV.dial PSR @17:52:22.09 Pulling 0.5ml of PSR.wash @17:52:22.10 PSR.select :wash @17:52:22.21 PS.seek 0.5ml @17:52:28.13 delay 5 seconds @17:52:33.14 PSR.select :air @17:52:33.26 PS.seek 2ml @17:52:49.28 PTV.dial :puck @17:52:49.97 PSV.dial PTV @17:52:50.80 PBV.dial :waste1 @17:52:52.09 PS.seek 1.5ml @17:52:58.41 delay 3 seconds @17:53:01.42 PBV.dial between PSV and waste1 @17:53:01.77 PS.seek 0ml @17:53:17.78 delay 5:00 @17:58:17.80 recoverPSRreagent08 @17:58:17.81 PTV.dial :puck @17:58:17.82 PBV.dial :waste1 @17:58:18.18 delay 30 seconds @17:58:48.20 PBV.dial between PSV and waste1 @17:58:48.55 PSR.select :air @17:58:48.57 PSV.dial PSR @17:58:49.28 PS.reconfigure :fast @17:58:49.32 PS.seek 2.5ml @17:59:11.96 PSV.dial PTV @17:59:12.68 delay 5 seconds @17:59:17.70 PSV.dial PSR @17:59:18.41 delay 5 seconds @17:59:23.43 PSV.dial PTV @17:59:24.13 delay 5 seconds @17:59:29.15 PSV.dial :air @17:59:29.87 PS.seek 0ml @17:59:52.61 PTV.dial :PRVmixing,via: PRV @17:59:53.91 PSV.dial :mixloop @17:59:54.35 PRV.dial :ab1 @17:59:55.09 PS.reconfigure :slow @17:59:55.13 PS.seek 0.15ml @17:59:58.74 delay 2 seconds @18:00:00.76 CBV.dial PRV @18:00:00.78
PRV.dial CBV @18:00:01.35 PS.seek 0.34ml @18:00:05.64 delay 2 seconds @18:00:07.66 CBV.dial between PRV and PSV @18:00:07.97 PRV.dial :air @18:00:08.40 PS.seek 0.9ml @18:00:20.24 PRV.dial :waste2 @18:00:20.95 PS.seek 0.5ml @18:00:29.55 addPMLReagent08 @18:00:29.56 PSV.dial :air @18:00:30.01 PS.reconfigure :default @18:00:30.05 PS.seek 2ml @18:00:46.14 PSV.dial :PML @18:00:46.58 PTV.dial :mixing @18:00:47.30 PBV.dial :waste1 @18:00:47.70 PS.seek 1.85ml @18:00:50.11 PBV.dial between PSV and waste1 @18:00:50.48 PS.seek 0ml @18:01:10.23 delay 2 seconds @18:01:12.25 Beginning samplePassC @18:01:12.27 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:01:12.43 Elbow.reconfigure :tight @18:01:12.47 Elbow.seek FlushPuck::Garage @18:01:13.79 Elbow.reconfigure :loose @18:01:13.88 Forearm.seek :garage @18:01:15.00 Hand.close @18:01:15.78 Forearm.seek :retract @18:01:16.88 Elbow.reconfigure :tight @18:01:16.93 Elbow.seek CC @18:01:19.84 Elbow.reconfigure :loose @18:01:19.94 Forearm.reconfigure :tight @18:01:19.98 Forearm.seek CC @18:01:21.28 CC.seek :closed @18:01:25.51 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.81 @18:01:25.58 Hand.open @18:01:26.35 Forearm.reconfigure :loose @18:01:26.39 Forearm.seek :retract @18:01:27.42 PSV.dial PSR @18:01:28.46 Pulling 1.5ml of PSR.flush @18:01:28.47 PSR.select :flush @18:01:28.59 PS.seek 1.5ml @18:01:44.61 delay 5 seconds @18:01:49.63 PSV.dial :waste1 @18:01:50.07 PS.seek 0ml @18:02:06.14 PSV.dial PSR @18:02:06.69 PSR.select :air @18:02:06.80 PS.seek 2.5ml @18:02:32.92 PRV.dial CBV,avoiding: :ab2 @18:02:33.41
CBV.dial PRV @18:02:34.98 PSV.dial :mixloop @18:02:35.99 CTV.dial :air,avoiding: :intake @18:02:36.00 PTV.dial :PRVmixing @18:02:36.74 PS.seek 0ml @18:03:02.95 PSV.dial :air @18:03:03.39 PS.seek 2.5ml @18:03:29.51 PSV.dial :mixloop @18:03:29.97 PS.seek 0ml @18:03:56.17 PSV.dial :air @18:03:56.61 PS.seek 2.5ml @18:04:22.76 PSV.dial :mixloop @18:04:23.20 PS.seek 0ml @18:04:49.36 Garaging FlushPuck in Collection Clamp @18:04:49.43 Elbow.reconfigure :tight @18:04:49.47 Elbow.seek CC @18:04:49.58 Elbow.reconfigure :loose @18:04:49.68 Forearm.seek CC @18:04:50.62 Hand.close @18:04:51.41 CC.seek :open @18:04:55.73 Forearm.seek :retract @18:04:56.76 Elbow.reconfigure :tight @18:04:56.81 Elbow.seek :garage @18:04:59.90 Elbow.reconfigure :loose @18:04:59.99 Forearm.reconfigure :tight @18:05:00.03 Forearm.seek :garage @18:05:01.17 Hand.open @18:05:01.96 Forearm.reconfigure :loose @18:05:02.00 Forearm.seek :retract @18:05:03.03 delayUntil "18:09:12.24" @18:05:03.05 Whole Cell Archive sampling 1000ml @18:05:03.07 Loading wcr puck from tube 2 into Collection Clamp @18:05:03.52 Elbow.reconfigure :tight @18:05:03.56 Elbow.seek 2 @18:05:05.54 Forearm.reconfigure :tight @18:05:05.58 Forearm.seek 2 + 700 counts @18:05:06.66 SE.reconfigure :pick @18:05:06.71 SE.seek :beamBreak @18:05:08.15 SE.configure :default @18:05:08.30 Elbow.seek 2 @18:05:08.68 Forearm.seek 2 + 300 counts @18:05:08.84 Forearm.reconfigure :loose @18:05:08.88 Hand.close @18:05:09.54 SE.seek :down @18:05:09.74 Forearm.seek :retract @18:05:10.69 SE.stop @18:05:10.80 Elbow.seek CC @18:05:12.44 Elbow.reconfigure :loose @18:05:12.54 Forearm.reconfigure :tight @18:05:12.59 Forearm.seek CC @18:05:13.94 CC.seek :closed @18:05:18.18 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.74 @18:05:18.19 Hand.open @18:05:18.96 Forearm.reconfigure :loose @18:05:19.01 Forearm.seek :retract @18:05:20.03 collectSample 1000ml w/bubble point of 35psi @18:05:20.04 Priming 7ml @18:05:20.05 Intake.select :close @18:05:20.06 Exhaust.select :close @18:05:20.07 SS.hold @18:05:20.73 Calibrating pressure sensors @18:05:20.75 CTV.dial :air @18:05:20.76 SSV.dial :spit,avoiding: :exhaust @18:05:20.78 SS.reconfigure :fast @18:05:20.82 SS.seek 5ml @18:05:24.02 SSV.dial CBV @18:05:24.05 CBV.dial SSV @18:05:25.35 delay 3.8 seconds @18:05:29.27 SSV.dial :spit @18:05:30.59 SS.seek 0ml @18:05:33.36 SSV.dial CBV @18:05:34.65 CTV.dial :intake @18:05:35.37 SSV.dial CBV @18:05:35.39 CBV.dial SSV @18:05:35.42 Allowing 40s for 7.0ml of remaining 7.0ml @18:05:35.43 Intake.select :open @18:05:37.58 Intake @15.0psia, vacuum from 9.5 to 12.5psi @18:05:37.60 SS.reconfigure :inhaling 2.14ml/s @18:05:37.64 SS.seek 7ml @18:05:43.45 Stroke completed -- waiting for pressure to equalize ... @18:05:43.46 SS.reconfigure :exhale @18:05:43.51 SS.hold @18:05:48.28 Intake.select :close @18:05:48.43 SSV.dial :exhaust @18:05:49.19 Exhaust.select :open @18:05:49.30 SS.seek 0ml @18:05:53.58 Exhaust.select :close @18:05:53.69 Sampling 1000.0ml @18:05:53.70 Intake.select :close @18:05:53.71 Exhaust.select :close @18:05:53.72 SS.reconfigure :default @18:05:53.76 SS.hold @18:05:53.95 CTV.dial :intake @18:05:53.97 SSV.dial CBV @18:05:53.99 CBV.dial SSV @18:05:54.75 Allowing 130s for 25.0ml of remaining 1000.0ml @18:05:54.76 Intake.select :open @18:05:56.91 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:05:56.93 SS.reconfigure :inhaling 2.14ml/s @18:05:56.98 SS.seek 25ml @18:06:02.24 Vacuum too high -- waiting up to 40 seconds @18:06:02.25 SS.reconfigure :exhale @18:06:02.30 SS.hold @18:06:03.03 Intake @11.9psia, vacuum from 6.4 to 9.4psi @18:06:03.05 SS.reconfigure :inhaling 1.93ml/s @18:06:03.09 SS.seek 25ml @18:06:03.24 Vacuum too high -- waiting up to 40 seconds @18:06:03.25 SS.reconfigure :exhale @18:06:03.29 SS.hold @18:06:05.36 Intake @14.1psia, vacuum from 8.6 to 11.6psi @18:06:05.38 SS.reconfigure :inhaling 1.74ml/s @18:06:05.42 SS.seek 25ml @18:06:09.46 Vacuum too high -- waiting up to 40 seconds @18:06:09.47 SS.reconfigure :exhale @18:06:09.51 SS.hold @18:06:10.97 Intake @13.4psia, vacuum from 7.9 to 10.9psi @18:06:10.99 SS.reconfigure :inhaling 1.57ml/s @18:06:11.03 SS.seek 25ml @18:06:14.14 Vacuum too high -- waiting up to 40 seconds @18:06:14.16 SS.reconfigure :exhale @18:06:14.20 SS.hold @18:06:15.98 Intake @14.0psia, vacuum from 8.5 to 11.5psi @18:06:16.00 SS.reconfigure :inhaling 1.41ml/s @18:06:16.04 SS.seek 25ml @18:06:20.68 Vacuum too high -- waiting up to 40 seconds @18:06:20.69 SS.reconfigure :exhale @18:06:20.73 SS.hold @18:06:23.06 Intake @14.3psia, vacuum from 8.8 to 11.8psi @18:06:23.08 SS.reconfigure :inhaling 1.27ml/s @18:06:23.12 SS.seek 25ml @18:06:27.18 Stroke completed -- waiting for pressure to equalize ... @18:06:27.20 SS.reconfigure :exhale @18:06:27.24 SS.hold @18:06:29.64 Intake.select :close @18:06:29.79 SSV.dial :exhaust @18:06:30.51 Exhaust.select :open @18:06:30.62 SS.seek 0ml @18:06:39.54 Exhaust.select :close @18:06:39.65 SSV.dial CBV @18:06:39.69 CBV.dial SSV @18:06:40.42 Allowing 130s for 25.0ml of remaining 975.0ml @18:06:40.43 Intake.select :open @18:06:42.59 Intake @15.0psia, vacuum from 9.5 to 12.5psi @18:06:42.61 SS.reconfigure :inhaling 1.27ml/s @18:06:42.65 SS.seek 25ml @18:06:50.39 Vacuum too high -- waiting up to 40 seconds @18:06:50.41 SS.reconfigure :exhale @18:06:50.45 SS.hold @18:06:51.79 Intake @13.9psia, vacuum from 8.4 to 11.4psi @18:06:51.81 SS.reconfigure :inhaling 1.14ml/s @18:06:51.86 SS.seek 25ml @18:06:58.08 Vacuum too high -- waiting up to 40 seconds @18:06:58.09 SS.reconfigure :exhale @18:06:58.13 SS.hold @18:06:59.85 Intake @14.0psia, vacuum from 8.5 to 11.5psi @18:06:59.87 SS.reconfigure :inhaling 1.03ml/s @18:06:59.92 SS.seek 25ml @18:07:12.41 Stroke completed -- waiting for pressure to equalize ... @18:07:12.43 SS.reconfigure :exhale @18:07:12.47 SS.hold @18:07:15.37 Intake.select :close @18:07:15.52 SSV.dial :exhaust @18:07:16.25 Exhaust.select :open @18:07:16.36 SS.seek 0ml @18:07:25.38 Exhaust.select :close @18:07:25.49 SSV.dial CBV @18:07:25.52 CBV.dial SSV @18:07:26.28 Allowing 130s for 25.0ml of remaining 950.0ml @18:07:26.29 Intake.select :open @18:07:28.44 Intake @14.9psia, vacuum from 9.4 to 12.4psi @18:07:28.46 SS.reconfigure :inhaling 1.03ml/s @18:07:28.51 SS.seek 25ml @18:07:39.74 Vacuum too high -- waiting up to 40 seconds @18:07:39.75 SS.reconfigure :exhale @18:07:39.79 SS.hold @18:07:40.76 Intake @13.6psia, vacuum from 8.1 to 11.1psi @18:07:40.77 SS.reconfigure :inhaling 0.93ml/s @18:07:40.82 SS.seek 25ml @18:07:57.86 Vacuum too high -- waiting up to 40 seconds @18:07:57.87 SS.reconfigure :exhale @18:07:57.92 SS.hold @18:07:59.56 Intake @13.3psia, vacuum from 7.8 to 10.8psi @18:07:59.58 SS.reconfigure :inhaling 0.84ml/s @18:07:59.63 SS.seek 25ml @18:08:01.55 Stroke completed -- waiting for pressure to equalize ... @18:08:01.57 SS.reconfigure :exhale @18:08:01.61 SS.hold @18:08:02.80 Intake.select :close @18:08:02.96 SSV.dial :exhaust @18:08:03.70 Exhaust.select :open @18:08:03.81 SS.seek 0ml @18:08:13.12 Exhaust.select :close @18:08:13.70 SSV.dial CBV @18:08:13.73 CBV.dial SSV @18:08:14.48 Allowing 130s for 25.0ml of remaining 925.0ml @18:08:14.49 Intake.select :open @18:08:16.65 Intake @14.8psia, vacuum from 9.3 to 12.3psi @18:08:16.67 SS.reconfigure :inhaling 0.84ml/s @18:08:16.71 SS.seek 25ml @18:08:25.26 Vacuum too high -- waiting up to 40 seconds @18:08:25.28 SS.reconfigure :exhale @18:08:25.32 SS.hold @18:08:26.62 Intake @14.2psia, vacuum from 8.7 to 11.7psi @18:08:26.64 SS.reconfigure :inhaling 0.76ml/s @18:08:26.68 SS.seek 25ml @18:08:34.08 Vacuum too high -- waiting up to 40 seconds @18:08:34.09 SS.reconfigure :exhale @18:08:34.13 SS.hold @18:08:36.19 Intake @15.2psia, vacuum from 9.7 to 12.7psi @18:08:36.21 SS.reconfigure :inhaling 0.69ml/s @18:08:36.25 SS.seek 25ml @18:08:44.42 Vacuum too high -- waiting up to 40 seconds @18:08:44.43 SS.reconfigure :exhale @18:08:44.48 SS.hold @18:08:47.48 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:08:47.50 SS.reconfigure :inhaling 0.61ml/s @18:08:47.55 SS.seek 25ml @18:08:55.17 Vacuum too high -- waiting up to 40 seconds @18:08:55.18 SS.reconfigure :exhale @18:08:55.22 SS.hold @18:09:01.74 SS.reconfigure :inhaling 0.56ml/s @18:09:01.79 SS.seek 25ml @18:09:06.64 Vacuum too high -- waiting up to 40 seconds @18:09:06.65 SS.reconfigure :exhale @18:09:06.69 SS.hold @18:09:12.30 recoverPRVreagent08 @18:09:12.31 PTV.dial :mixing @18:09:13.03 PBV.dial :waste1 @18:09:13.39 delay 30 seconds @18:09:43.42 PBV.dial between PSV and waste1 @18:09:43.79 PSV.dial :air @18:09:44.24 PS.reconfigure :fast @18:09:44.29 PS.seek 2.5ml @18:09:46.88 SS.reconfigure :default @18:09:46.93 SS.hold @18:09:47.03 Intake.select :close @18:09:47.68 ShallowSampler::Clogged in WCR -- Intake @14.8psia, & Outlet @4.2psia -- no progress after 97.3ml @18:09:47.69 SSV.dial :exhaust @18:09:48.41 Exhaust.select :open @18:09:48.52 SS.reconfigure :exhale @18:09:48.57 SS.seek 0ml @18:09:57.27 Exhaust.select :close @18:09:57.38 Sampled 97.3ml @18:09:57.39 Drying puck before fixing for 20:00 @18:09:57.40 CBV.dial :waste2b @18:09:58.11 delay 1:00 @18:10:07.47 PSV.dial :mixloop @18:10:07.92 delay 2 seconds @18:10:09.94 PSV.dial :air @18:10:10.39 delay 2 seconds @18:10:12.41 PSV.dial :mixloop @18:10:12.86 delay 2 seconds @18:10:14.88 PSV.dial :air @18:10:15.33 PS.seek 0ml @18:10:37.91 washCycleDA #1 of 2 @18:10:37.92 PSR.select :air @18:10:37.93 PSV.dial PSR @18:10:39.24 Pulling 0.5ml of PSR.wash @18:10:39.25 PSR.select :wash @18:10:39.36 PS.reconfigure :default @18:10:39.40 PS.seek 0.5ml @18:10:45.35 delay 5 seconds @18:10:50.36 PSR.select :air @18:10:50.48 PS.seek 2ml @18:10:58.13 CSV.dial :air @18:10:58.58 CS.reconfigure :fast @18:10:58.62 CS.seek 10ml @18:11:06.47 PTV.dial :puck @18:11:07.20 PSV.dial PTV @18:11:07.92 PBV.dial :waste1 @18:11:08.34 PS.seek 1.5ml @18:11:14.31 delay 3 seconds @18:11:17.33 PBV.dial between PSV and waste1 @18:11:17.69 PS.seek 0ml @18:11:21.26 Pumping wcr puck from tube 2 up to 35psi... @18:11:21.28 CBV.dial SSV @18:11:22.54 CBV.dial between CSV and waste2b @18:11:23.45 CSV.dial between air and CTV @18:11:23.77 CS.seek 7.735ml @18:11:29.55 CTV.dial CSV,avoiding: :air @18:11:29.58 CSV.dial CTV @18:11:30.33 CS.reconfigure :pressurize @18:11:30.38 CS.seek 0ml @18:11:33.78 delay 2:00 @18:11:49.81 CBV.dial :waste2b @18:11:50.14 CS.reconfigure :default @18:11:50.18 CS.seek 0ml @18:11:51.45 delay 1:00 @18:12:51.47 CTV.dial :air,avoiding: :intake @18:12:52.73 Fix #1 of 2 (with RNAlater) for 10:00 @18:12:52.75 CSV.dial CSR @18:12:53.23 Pulling 2ml of CSR.RNAlater @18:12:53.24 CSR.select :RNAlater @18:12:53.36 CS.seek 2ml @18:12:59.23 delay 5 seconds @18:13:04.25 CSR.select :air @18:13:04.36 CS.reconfigure :fast @18:13:04.41 CS.seek 10ml @18:13:22.60 Pumping wcr puck from tube 2 up to 35psi... @18:13:22.62 CBV.dial SSV @18:13:23.88 CBV.dial between CSV and waste2b @18:13:24.80 CSV.dial between air and CTV @18:13:25.39 CS.seek 7.418ml @18:13:31.92 CTV.dial CSV,avoiding: :air @18:13:31.96 CSV.dial CTV @18:13:33.23 CS.reconfigure :pressurize @18:13:33.28 CS.seek 0ml @18:13:33.80 recoverPSRreagent08 @18:13:33.81 PTV.dial :puck @18:13:33.82 PBV.dial :waste1 @18:13:34.17 delay 30 seconds @18:13:51.12 delay 10:00 @18:14:04.19 PBV.dial between PSV and waste1 @18:14:04.54 PSR.select :air @18:14:04.56 PSV.dial PSR @18:14:05.28 PS.reconfigure :fast @18:14:05.33 PS.seek 2.5ml @18:14:28.24 PSV.dial PTV @18:14:29.40 delay 5 seconds @18:14:34.42 PSV.dial PSR @18:14:35.14 delay 5 seconds @18:14:40.15 PSV.dial PTV @18:14:40.87 delay 5 seconds @18:14:45.89 PSV.dial :air @18:14:46.61 PS.seek 0ml @18:15:09.24 washCycleDA #2 of 2 @18:15:09.25 PSR.select :air @18:15:09.27 PSV.dial PSR @18:15:10.56 Pulling 0.5ml of PSR.wash @18:15:10.57 PSR.select :wash @18:15:10.68 PS.reconfigure :default @18:15:10.73 PS.seek 0.5ml @18:15:16.59 delay 5 seconds @18:15:21.61 PSR.select :air @18:15:21.72 PS.seek 2ml @18:15:37.74 PTV.dial :puck @18:15:37.75 PSV.dial PTV @18:15:38.44 PBV.dial :waste1 @18:15:38.86 PS.seek 1.5ml @18:15:44.71 delay 3 seconds @18:15:47.73 PBV.dial between PSV and waste1 @18:15:48.09 PS.seek 0ml @18:16:04.12 delay 2:00 @18:18:04.14 recoverPSRreagent08 @18:18:04.15 PTV.dial :puck @18:18:04.16 PBV.dial :waste1 @18:18:04.52 delay 30 seconds @18:18:34.55 PBV.dial between PSV and waste1 @18:18:34.89 PSR.select :air @18:18:34.91 PSV.dial PSR @18:18:35.63 PS.reconfigure :fast @18:18:35.68 PS.seek 2.5ml @18:18:58.20 PSV.dial PTV @18:18:58.93 delay 5 seconds @18:19:03.94 PSV.dial PSR @18:19:04.66 delay 5 seconds @18:19:09.68 PSV.dial PTV @18:19:10.39 delay 5 seconds @18:19:15.41 PSV.dial :air @18:19:16.13 PS.seek 0ml @18:19:38.74 getPMLReagent08 @18:19:38.75 PRV.dial :air @18:19:39.19 delay 5 seconds @18:19:44.21 PTV.dial :PRVmixing @18:19:45.50 delay 5 seconds @18:19:50.52 PRV.dial :ab2 @18:19:50.97 PTV.dial :PRVmixing @18:19:50.98 PSV.dial :mixloop @18:19:51.43 PS.reconfigure :default @18:19:51.47 PS.seek 0.3ml @18:19:55.42 delay 2 seconds @18:19:57.44 PRV.dial :air @18:19:57.97 PS.seek 0.55ml @18:20:01.42 addPMLReagent08 @18:20:01.43 PSV.dial :air @18:20:01.88 PS.seek 2ml @18:20:17.58 PSV.dial :PML @18:20:18.02 PTV.dial :mixing @18:20:18.75 PBV.dial :waste1 @18:20:19.15 PS.seek 1.85ml @18:20:21.55 PBV.dial between PSV and waste1 @18:20:21.91 PS.seek 0ml @18:20:41.70 delay 2 seconds @18:20:43.72 delay 8:00 @18:23:51.14 CBV.dial :waste2b @18:23:51.45 CS.reconfigure :default @18:23:51.49 CS.seek 0ml @18:23:53.59 delay 1:00 @18:24:53.61 CTV.dial :air,avoiding: :intake @18:24:54.89 Fix #2 of 2 (with RNAlater) for 10:00 @18:24:54.90 CSV.dial CSR @18:24:55.37 Pulling 2ml of CSR.RNAlater @18:24:55.38 CSR.select :RNAlater @18:24:55.50 CS.seek 2ml @18:25:01.40 delay 5 seconds @18:25:06.42 CSR.select :air @18:25:06.53 CS.reconfigure :fast @18:25:06.57 CS.seek 10ml @18:25:24.87 Pumping wcr puck from tube 2 up to 35psi... @18:25:24.88 CBV.dial SSV @18:25:26.13 CBV.dial between CSV and waste2b @18:25:27.05 CSV.dial between air and CTV @18:25:27.64 CS.seek 7.377ml @18:25:34.24 CTV.dial CSV,avoiding: :air @18:25:34.27 CSV.dial CTV @18:25:35.57 CS.reconfigure :pressurize @18:25:35.62 CS.seek 0ml @18:25:52.98 delay 10:00 @18:28:43.74 recoverPRVreagent08 @18:28:43.75 PTV.dial :mixing @18:28:43.76 PBV.dial :waste1 @18:28:44.12 delay 30 seconds @18:29:14.14 PBV.dial between PSV and waste1 @18:29:14.49 PSV.dial :air @18:29:15.42 PS.reconfigure :fast @18:29:15.46 PS.seek 2.5ml @18:29:38.41 PSV.dial :mixloop @18:29:38.86 delay 2 seconds @18:29:40.88 PSV.dial :air @18:29:41.33 delay 2 seconds @18:29:43.35 PSV.dial :mixloop @18:29:43.80 delay 2 seconds @18:29:45.82 PSV.dial :air @18:29:46.28 PS.seek 0ml @18:30:08.94 washCycleDA #1 of 3 @18:30:08.95 PSR.select :air @18:30:08.97 PSV.dial PSR @18:30:10.28 Pulling 0.5ml of PSR.wash @18:30:10.29 PSR.select :wash @18:30:10.40 PS.reconfigure :default @18:30:10.44 PS.seek 0.5ml @18:30:16.41 delay 5 seconds @18:30:21.43 PSR.select :air @18:30:21.54 PS.seek 2ml @18:30:37.53 PTV.dial :puck @18:30:38.24 PSV.dial PTV @18:30:38.94 PBV.dial :waste1 @18:30:39.36 PS.seek 1.5ml @18:30:45.28 delay 3 seconds @18:30:48.30 PBV.dial between PSV and waste1 @18:30:48.67 PS.seek 0ml @18:31:04.69 delay 2:00 @18:33:04.71 recoverPSRreagent08 @18:33:04.72 PTV.dial :puck @18:33:04.73 PBV.dial :waste1 @18:33:05.08 delay 30 seconds @18:33:35.10 PBV.dial between PSV and waste1 @18:33:35.45 PSR.select :air @18:33:35.47 PSV.dial PSR @18:33:36.19 PS.reconfigure :fast @18:33:36.24 PS.seek 2.5ml @18:33:59.21 PSV.dial PTV @18:33:59.94 delay 5 seconds @18:34:04.95 PSV.dial PSR @18:34:05.67 delay 5 seconds @18:34:10.69 PSV.dial PTV @18:34:11.40 delay 5 seconds @18:34:16.42 PSV.dial :air @18:34:17.14 PS.seek 0ml @18:34:40.06 washCycleDA #2 of 3 @18:34:40.07 PSR.select :air @18:34:40.09 PSV.dial PSR @18:34:41.37 Pulling 0.5ml of PSR.wash @18:34:41.38 PSR.select :wash @18:34:41.50 PS.reconfigure :default @18:34:41.54 PS.seek 0.5ml @18:34:47.50 delay 5 seconds @18:34:52.52 PSR.select :air @18:34:52.63 PS.seek 2ml @18:35:08.74 PTV.dial :puck @18:35:08.76 PSV.dial PTV @18:35:09.45 PBV.dial :waste1 @18:35:09.87 PS.seek 1.5ml @18:35:15.71 delay 3 seconds @18:35:18.73 PBV.dial between PSV and waste1 @18:35:19.10 PS.seek 0ml @18:35:35.15 delay 2:00 @18:35:53.01 CBV.dial :waste2b @18:35:53.32 CS.reconfigure :default @18:35:53.37 CS.seek 0ml @18:35:55.76 delay 1:00 @18:36:55.78 CTV.dial :air,avoiding: :intake @18:37:35.18 recoverPSRreagent08 @18:37:35.19 PTV.dial :puck @18:37:35.20 PBV.dial :waste1 @18:37:35.55 delay 30 seconds @18:38:05.58 PBV.dial between PSV and waste1 @18:38:05.93 PSR.select :air @18:38:05.95 PSV.dial PSR @18:38:06.67 PS.reconfigure :fast @18:38:06.71 PS.seek 2.5ml @18:38:29.61 PSV.dial PTV @18:38:30.33 delay 5 seconds @18:38:35.35 PSV.dial PSR @18:38:36.07 delay 5 seconds @18:38:41.09 PSV.dial PTV @18:38:41.80 delay 5 seconds @18:38:46.82 PSV.dial :air @18:38:47.54 PS.seek 0ml @18:39:10.46 washCycleDA #3 of 3 @18:39:10.47 PSR.select :air @18:39:10.48 PSV.dial PSR @18:39:11.90 Pulling 0.5ml of PSR.wash @18:39:11.91 PSR.select :wash @18:39:12.02 PS.reconfigure :default @18:39:12.07 PS.seek 0.5ml @18:39:18.08 delay 5 seconds @18:39:23.10 PSR.select :air @18:39:23.21 PS.seek 2ml @18:39:39.20 PTV.dial :puck @18:39:39.22 PSV.dial PTV @18:39:39.94 PBV.dial :waste1 @18:39:40.34 PS.seek 1.5ml @18:39:46.20 delay 3 seconds @18:39:49.22 PBV.dial between PSV and waste1 @18:39:49.59 PS.seek 0ml @18:40:05.61 delay 2:00 @18:42:05.64 recoverPSRreagent08 @18:42:05.65 PTV.dial :puck @18:42:05.66 PBV.dial :waste1 @18:42:06.01 delay 30 seconds @18:42:36.04 PBV.dial between PSV and waste1 @18:42:36.39 PSR.select :air @18:42:36.88 PSV.dial PSR @18:42:37.60 PS.reconfigure :fast @18:42:37.64 PS.seek 2.5ml @18:43:00.64 PSV.dial PTV @18:43:01.36 delay 5 seconds @18:43:06.38 PSV.dial PSR @18:43:07.09 delay 5 seconds @18:43:12.11 PSV.dial PTV @18:43:12.83 delay 5 seconds @18:43:17.84 PSV.dial :air @18:43:18.56 PS.seek 0ml @18:43:41.51 Gate.power :camera,:ON @18:43:41.52 Mixing DA Substrates @18:43:41.53 PSR.select :air @18:43:41.55 PSV.dial PSR @18:43:43.08 Pulling 0.25ml of PSR.sub1 @18:43:43.11 PSR.select :sub1 @18:43:43.40 PS.reconfigure :default @18:43:43.56 PS.seek 0.25ml @18:43:47.01 delay 5 seconds @18:43:52.08 Pulling 0.25ml of PSR.sub2 @18:43:52.09 PSR.select :sub2 @18:43:52.21 PS.seek 0.5ml @18:43:55.54 delay 5 seconds @18:44:00.56 PSR.select :air @18:44:00.67 PS.seek 2ml @18:44:16.73 PTV.dial :puck @18:44:16.74 PSV.dial PTV @18:44:17.45 PBV.dial :waste1 @18:44:17.88 PS.seek 1.5ml @18:44:23.85 delay 3 seconds @18:44:26.87 PBV.dial between PSV and waste1 @18:44:27.24 PS.seek 0ml @18:44:43.32 delay 10 seconds @18:44:53.35 recoverPSRreagent08 @18:44:53.36 PTV.dial :puck @18:44:53.37 PBV.dial :waste1 @18:44:53.71 delay 30 seconds @18:45:23.73 PBV.dial between PSV and waste1 @18:45:24.09 PSR.select :air @18:45:24.10 PSV.dial PSR @18:45:24.95 PS.reconfigure :fast @18:45:25.00 PS.seek 2.5ml @18:45:48.22 PSV.dial PTV @18:45:48.93 delay 5 seconds @18:45:53.95 PSV.dial PSR @18:45:54.66 delay 5 seconds @18:45:59.68 PSV.dial PTV @18:46:00.40 delay 5 seconds @18:46:05.42 PSV.dial :air @18:46:06.14 PS.seek 0ml @18:46:28.95 Additional air clear @18:46:28.96 PSR.select :air @18:46:28.97 PSV.dial PSR @18:46:30.28 Pulling 2ml of PSR.air @18:46:30.29 PSR.select :air @18:46:30.31 PS.reconfigure :default @18:46:30.35 PS.seek 2ml @18:46:51.41 delay 5 seconds @18:46:56.43 PTV.dial :puck @18:46:56.45 PSV.dial PTV @18:46:57.16 PBV.dial :waste1 @18:46:57.58 PS.seek 1.5ml @18:47:03.60 delay 3 seconds @18:47:06.62 PBV.dial between PSV and waste1 @18:47:06.98 delay 1 second @18:47:07.99 PSV.dial :waste1 @18:47:08.99 PS.seek 0ml @18:47:25.09 Processing Puck Heater at 28.2C before imaging @18:47:25.10 PH.coast @18:47:25.22 Imaging da puck in Processing Clamp while storing it to tube 1 @18:47:25.38 SC.reconfigure :tight @18:47:25.42 SC.seek 1 @18:47:29.04 SC.configure :limp @18:47:29.23 Elbow.reconfigure :tight @18:47:29.28 Elbow.seek 1 @18:47:30.95 Forearm.reconfigure :tight @18:47:30.99 Forearm.seek 1 + 700 counts @18:47:31.99 SE.reconfigure :pick @18:47:32.04 SE.seek :beamBreak @18:47:34.78 SE.configure :default @18:47:34.92 Elbow.reconfigure :limp @18:47:34.97 Forearm.reconfigure :loose @18:47:35.01 Forearm.seek 1 @18:47:35.43 Elbow.hold @18:47:35.49 Elbow.reconfigure :tight @18:47:35.58 SE.seek 5.23 Pucks below Top Plate @18:47:36.11 Forearm.force -20 @18:47:36.15 Forearm.seek 1 + 319 counts @18:47:36.47 Forearm.reconfigure :tight @18:47:36.51 Forearm.seek 1 + 700 counts @18:47:36.75 SE.seek 6.23 Pucks below Top Plate @18:47:37.29 Forearm.reconfigure :loose @18:47:37.33 Forearm.seek :retract @18:47:38.31 Elbow.seek PC @18:47:41.07 Elbow.reconfigure :loose @18:47:41.59 Forearm.seek PC @18:47:42.74 Hand.close @18:47:43.45 PC.seek :open @18:47:47.48 Forearm.seek :retract @18:47:48.51 Elbow.reconfigure :tight @18:47:48.55 Elbow.seek 1 @18:47:51.15 Forearm.reconfigure :tight @18:47:51.20 Forearm.seek 1 + 700 counts @18:47:52.18 Hand.open @18:47:53.01 SE.seek 5.88 Pucks below Top Plate @18:47:53.30 SE.reconfigure :seal @18:47:53.34 SE.seek 4.78 Pucks below Top Plate @18:47:53.91 Lights, camera, ACTION! @18:47:53.94 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22oct0618h87ml.tif @18:48:10.70 SX MX5/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 13.035 seconds /var/log/esp/da22oct0618h87ml.tif: TIFF Upload Complete @18:48:10.73 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22oct0618h87ml15s.tif @18:48:13.39 Waiting >20 seconds for da to relinquish FlushPuck @18:48:26.50 SX MX5/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22oct0618h87ml15s.tif: TIFF Upload Complete @18:48:26.53 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da22oct0618h87ml25s.tif @18:48:52.24 SX MX5/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da22oct0618h87ml25s.tif: TIFF Upload Complete @18:48:52.26 Gate.power :camera,:OFF @18:48:52.31 DAfans.email "13.035 sec auto-exposure",Subject: "da22oct0618h87ml images!" @18:48:52.59 Elbow.seek 1 @18:48:52.77 Forearm.seek 1 + 700 counts @18:48:52.85 SE.reconfigure :gently @18:48:52.89 SE.seek 5.49 Pucks below Top Plate @18:48:53.61 Elbow.reconfigure :limp @18:48:53.67 Forearm.reconfigure :loose @18:48:53.71 Forearm.seek 1 @18:48:54.13 Elbow.hold @18:48:54.20 Elbow.reconfigure :tight @18:48:54.25 SE.reconfigure :default @18:48:54.29 SE.seek 5.68 Pucks below Top Plate @18:48:54.71 Forearm.force -20 @18:48:54.76 Forearm.seek 1 + 319 counts @18:48:55.20 Forearm.reconfigure :tight @18:48:55.24 Forearm.seek 1 + 700 counts @18:48:55.52 SE.seek :down @18:48:56.70 Forearm.reconfigure :loose @18:48:56.74 Forearm.seek :retract @18:48:57.85 SE.stop @18:48:57.97 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:48:58.21 Elbow.seek FlushPuck::Garage @18:49:00.29 Elbow.reconfigure :loose @18:49:00.40 Forearm.seek :garage @18:49:01.55 Hand.close @18:49:02.44 Forearm.seek :retract @18:49:03.52 Elbow.reconfigure :tight @18:49:03.62 Elbow.seek PC @18:49:04.95 Elbow.reconfigure :loose @18:49:05.05 Forearm.reconfigure :tight @18:49:05.09 Forearm.seek PC @18:49:06.60 PC.seek :closed @18:49:10.55 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.44 @18:49:10.62 Hand.open @18:49:12.02 Forearm.reconfigure :loose @18:49:12.10 Forearm.seek :retract @18:49:13.29 PSR.select :air @18:49:13.35 Storing wcr puck in Collection Clamp to tube 1 @18:49:13.39 PSV.dial PSR @18:49:13.77 SC.reconfigure :tight @18:49:13.82 SC.seek 1 @18:49:13.92 SC.configure :limp @18:49:14.11 Pulling 0.5ml of PSR.flush @18:49:14.15 PSR.select :flush @18:49:14.38 Elbow.reconfigure :tight @18:49:14.46 Elbow.seek 1 @18:49:14.58 PS.seek 0.5ml @18:49:17.15 Forearm.reconfigure :tight @18:49:17.20 Forearm.seek 1 + 700 counts @18:49:18.29 SE.reconfigure :pick @18:49:18.34 SE.seek :beamBreak @18:49:20.44 delay 5 seconds @18:49:21.16 SE.configure :default @18:49:21.47 Elbow.reconfigure :limp @18:49:21.52 Forearm.reconfigure :loose @18:49:21.56 Forearm.seek 1 @18:49:21.96 Elbow.hold @18:49:22.03 Elbow.reconfigure :tight @18:49:22.13 SE.seek 6.25 Pucks below Top Plate @18:49:22.58 Forearm.force -20 @18:49:22.61 Forearm.seek 1 + 320 counts @18:49:22.97 Forearm.reconfigure :tight @18:49:23.02 Forearm.seek 1 + 700 counts @18:49:23.39 SE.seek 7.25 Pucks below Top Plate @18:49:24.04 Forearm.reconfigure :loose @18:49:24.12 Forearm.seek :retract @18:49:25.24 Elbow.seek CC @18:49:25.48 PSR.select :air @18:49:25.65 PS.seek 2.5ml @18:49:26.97 Elbow.reconfigure :loose @18:49:27.25 Forearm.seek CC @18:49:28.28 Hand.close @18:49:29.10 CC.seek :open @18:49:33.94 Forearm.seek :retract @18:49:35.04 Elbow.reconfigure :tight @18:49:35.14 Elbow.seek 1 @18:49:37.06 Elbow.reconfigure :limp @18:49:37.16 Forearm.seek 1 @18:49:38.37 Elbow.hold @18:49:38.50 Elbow.reconfigure :tight @18:49:38.58 Hand.open @18:49:39.59 Forearm.force -20 @18:49:39.69 Forearm.seek 1 + 320 counts @18:49:40.32 Forearm.reconfigure :tight @18:49:40.41 Forearm.seek 1 + 700 counts @18:49:40.82 SE.seek :down @18:49:42.07 Forearm.reconfigure :loose @18:49:42.20 Forearm.seek :retract @18:49:43.08 SE.stop @18:49:43.24
Starting finalPurge @18:49:46.80 delay 1 second @18:49:47.84 PRV.dial :waste2,avoiding: :ab2 @18:49:49.69 PTV.dial :PRVmixing @18:49:50.96 PSV.dial :mixloop @18:49:52.01 PS.seek :empty @18:50:03.17
Waiting >20 seconds for psFlush to relinquish FlushPuck @18:50:04.08 upsync @18:50:18.22 PSR.select :air @18:50:18.24 PSV.dial PSR @18:50:19.74 Pulling 0.5ml of PSR.flush @18:50:19.75 PSR.select :flush @18:50:19.87 PS.seek 0.5ml @18:50:25.82 delay 5 seconds @18:50:30.84 PSR.select :air @18:50:30.95 PS.seek 2.5ml @18:50:52.06 delay 1 second @18:50:53.08 PBV.dial :waste1 @18:50:53.44 PTV.dial :mixing @18:50:54.14 PSV.dial :mixloop @18:50:55.13 PS.seek :empty @18:51:21.34 PSV.dial PSR @18:51:22.32 PSR.select :flush @18:51:22.44 PS.seek 1ml @18:51:33.46 PSR.select :air @18:51:33.58 PS.seek 2.5ml @18:51:49.65 PSV.dial PTV @18:51:50.37 PTV.dial :puck @18:51:51.07 PBV.dial :waste1 @18:51:51.09 PS.seek 0ml @18:52:17.28 PSV.dial PSR @18:52:18.00 PSR.select :flush @18:52:18.12 PS.seek 0.5ml @18:52:23.99 PSR.select :air @18:52:24.11 PS.seek 2.5ml @18:52:45.18 PSV.dial PTV @18:52:46.01 PBV.dial PSV @18:52:46.55 PS.seek 0ml @18:53:12.77 PSV.dial PBV @18:53:13.33 PS.seek 2.5ml @18:53:39.59 PSV.dial :waste1 @18:53:40.31 PS.seek 0ml @18:54:06.49 PSV.dial PSR @18:54:07.05 PSR.select :air @18:54:07.08 PS.seek 2.5ml @18:54:33.27 PSV.dial PTV @18:54:33.99 PBV.dial PSV @18:54:34.01 PS.seek 0ml @18:55:00.24 PSV.dial PBV @18:55:00.81 PS.seek 2.5ml @18:55:26.93 PSV.dial :waste1 @18:55:27.65 PS.seek 0ml @18:55:53.86 PSV.dial PSR @18:55:54.30 PSR.select :flush @18:55:54.42 PS.seek 1ml @18:56:05.43 PSR.select :air @18:56:05.54 PS.seek 2.5ml @18:56:21.68 PSV.dial PTV @18:56:22.38 PTV.dial :puck @18:56:22.39 PBV.dial :waste1 @18:56:22.93 PS.seek 0ml @18:56:49.14 PSV.dial PSR @18:56:49.86 PSR.select :flush @18:56:49.98 PS.seek 0.5ml @18:56:55.86 PSR.select :air @18:56:55.97 PS.seek 2.5ml @18:57:17.05 PSV.dial PTV @18:57:17.77 PBV.dial PSV @18:57:18.31 PS.seek 0ml @18:57:44.45 PSV.dial PBV @18:57:44.91 PS.seek 2.5ml @18:58:11.11 PSV.dial :waste1 @18:58:11.81 PS.seek 0ml @18:58:37.95 PSV.dial PSR @18:58:38.39 PSR.select :air @18:58:38.42 PS.seek 2.5ml @18:59:04.64 PSV.dial PTV @18:59:05.34 PBV.dial PSV @18:59:05.36 PS.seek 0ml @18:59:31.55 PSV.dial PBV @18:59:32.12 PS.seek 2.5ml @18:59:58.23 PSV.dial :waste1 @18:59:58.96 PS.seek 0ml @19:00:25.08
Loading FlushPuck into Collection Clamp from Processing Clamp @19:00:25.26 Elbow.seek PC @19:00:27.85 Elbow.reconfigure :loose @19:00:27.94 Forearm.seek PC @19:00:29.03 Hand.close @19:00:29.67 PC.seek :open @19:00:33.62 Forearm.seek :retract @19:00:34.66 Elbow.reconfigure :tight @19:00:34.70 Elbow.seek CC @19:00:38.70 Elbow.reconfigure :loose @19:00:38.79 Forearm.reconfigure :tight @19:00:38.83 Forearm.seek CC @19:00:40.20 CC.seek :closed @19:00:44.42 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.77 @19:00:44.49 Hand.open @19:00:45.23 Forearm.reconfigure :loose @19:00:45.27 Forearm.seek :retract @19:00:46.31 Flushing the Collection Valve::Manifold @19:00:46.43 CSV.dial CSR @19:00:46.90 Pulling 5ml of CSR.flush CSR.select :flush @19:00:47.02 CS.seek 5ml @19:01:00.41 delay 5 seconds @19:01:05.43 CSR.select :air @19:01:05.58 CS.reconfigure :fast @19:01:05.63 CS.seek 7.5ml @19:01:11.96 CSV.dial :waste @19:01:13.22 CS.reconfigure :default @19:01:13.26 CS.seek 0ml @19:01:32.97 Flushing lower manifold's waste1 port @19:01:33.08 CSV.dial :flush @19:01:33.52 CS.reconfigure :fast @19:01:33.56 CS.seek 5ml @19:01:45.28 delay 5 seconds @19:01:50.30 CSV.dial :air @19:01:50.79 CS.seek 7ml @19:01:55.97 CSV.dial CTV @19:01:55.99
CTV.dial CSV @19:01:57.29 CBV.dial :waste1 @19:01:58.02 CS.seek 0ml @19:02:14.12 Flushing lower manifold's waste2 port @19:02:14.24 CSV.dial :flush @19:02:14.95 CS.seek 5ml @19:02:26.65 delay 5 seconds @19:02:31.67 CSV.dial :air @19:02:32.18 CS.seek 6.999ml @19:02:37.53 CSV.dial CTV @19:02:37.55
CTV.dial CSV @19:02:38.10 CBV.dial :waste2 @19:02:39.11 CS.seek 0ml @19:02:55.03 Flushing lower manifold's waste2b port @19:02:55.14 CSV.dial :flush @19:02:55.84 CS.seek 5ml @19:03:07.62 delay 5 seconds @19:03:12.64 CSV.dial :air @19:03:13.15 CS.seek 7.001ml @19:03:18.46 CSV.dial CTV @19:03:18.49
CTV.dial CSV @19:03:18.92 CBV.dial :waste2b @19:03:19.91 CS.seek 0ml @19:03:35.84 Disinfecting external Intake with kill @19:03:35.95 CSV.dial CSR @19:03:36.44 Pulling 8ml of CSR.kill @19:03:36.45 CSR.select :kill @19:03:36.56 CS.seek 8ml @19:03:54.75 delay 5 seconds @19:03:59.76 CSR.select :air @19:03:59.88 CS.seek 10ml @19:04:05.19 CSV.dial CBV @19:04:05.21
CBV.dial CSV @19:04:06.24 CTV.dial :intake @19:04:06.96 Intake.select :open @19:04:07.07 CS.seek 2ml @19:04:25.21 Intake.select :close @19:04:25.31 CTV.dial :air @19:04:26.04 CS.reconfigure :default @19:04:26.08 CS.seek 0ml @19:04:31.96 CSV.dial CSR @19:04:33.00 Pulling 5ml of CSR.flush @19:04:33.01 CSR.select :flush @19:04:33.12 CS.seek 5ml @19:04:46.56 delay 5 seconds @19:04:51.57 CSR.select :air @19:04:51.68 CS.seek 10ml @19:05:05.11 CSV.dial CBV @19:05:06.09 CS.seek 0ml @19:05:32.18 Garaging FlushPuck in Collection Clamp @19:05:32.25 Elbow.reconfigure :tight @19:05:32.29 Elbow.seek CC @19:05:32.40 Elbow.reconfigure :loose @19:05:32.49 Forearm.seek CC @19:05:33.44 Hand.close @19:05:34.61 CC.seek :open @19:05:38.98 Forearm.seek :retract @19:05:40.01 Elbow.reconfigure :tight @19:05:40.05 Elbow.seek :garage @19:05:43.11 Elbow.reconfigure :loose @19:05:43.20 Forearm.reconfigure :tight @19:05:43.24 Forearm.seek :garage @19:05:44.34 Hand.open @19:05:45.10 Forearm.reconfigure :loose @19:05:45.14 Forearm.seek :retract @19:05:46.18 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 17:13:36 @19:05:46.23 Asleep after completing DA_WCR_10622 assays @19:05:46.25 GoodNews.email "{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:02:42, 25.2C, 16% humidity, 15.7psia, 13.972V, 0.361A, 0.361A avg, 5.05W\nSampled 97.3ml",Subject: "Asleep after completing DA_WCR_10622 assays" @19:06:21.28
Uploading Logs @19:06:25.25 upsync @19:55:02.43UTC13-Oct-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @19:55:05.07 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @19:55:09.12 Polling Can every 10:00 @19:55:09.17 Cleanly exited at 19:35:53UTC13-Oct-22 @19:55:09.20 Loading /home/esp/esp2/mission/DA_WCR_101322.rb mission @19:55:09.26 Preparing to sleep until + 00:00:05 @19:55:09.27 GoodNews.email "Starting DA_WCR_101322 w/pucks in tube 2\n{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:55:09, 24.5C, 16% humidity, 15.7psia, 14.065V, 0.202A, 0.196A avg, 2.84W",Subject: "DA_WCR_101322 begun" @19:55:09.33
Asleep til + 00:00:05 @19:55:09.35 Continuing at 19:55 @19:55:09.63 Gate.power :core,:OFF @19:55:09.65 Gate.power :raw,:OFF @19:55:09.66 Shutdown @19:55:14.23
Powering core bus @19:55:14.30 GoodNews.email "Then, in tube 2: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_101322.rb:14\n{1 => 0,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:55:09, 24.5C, 16% humidity, 15.7psia, 14.065V, 0.202A, 0.196A avg, 2.84W",Subject: "Asleep until 19:55" @19:55:19.63
Gate.power :raw,:ON @19:55:20.93 CS.configure :default @19:55:21.13 PS.configure :default @19:55:21.31 SS.configure :default @19:55:21.48 CC.configure :default @19:55:21.66 PC.configure :default @19:55:21.87 Forearm.configure :loose @19:55:22.03 Elbow.configure :loose @19:55:22.25 SC.configure :loose @19:55:22.43 SE.configure :default @19:55:22.49 CH.coast @19:55:22.61 CH.configure :default @19:55:22.67 PH.coast @19:55:22.80 PH.configure :default @19:55:22.86 All dwarf controllers running firmware version 3.85 @19:55:23.00 Valve.reset @19:55:23.51 Intake.select :close @19:55:23.55 Exhaust.select :close @19:55:23.57 CTV.dial :air @19:55:23.73 PSV.dial :air @19:55:23.93 CSV.dial :waste1 @19:55:23.99
Forearm.reconfigure :loose @19:55:24.14 Forearm.home.seek :retract @19:55:24.51 SSV.dial :spit,avoiding: :exhaust @19:55:24.83 PS.reconfigure :default @19:55:24.91 PS.home.seek 0ml @19:55:25.05 Hand.close @19:55:25.13
Elbow.jog 1500 @19:55:25.38 CS.reconfigure :fast @19:55:25.46 SS.reconfigure :default @19:55:25.63 CS.home.seek 0ml @19:55:25.71 SS.home.seek 0ml @19:55:25.95
Elbow.reconfigure :loose @19:55:26.03 Elbow.home.seek :clear @19:55:30.57 SE.reconfigure :default @19:55:30.67 SE.home.seek :down @19:55:31.39 SC.reconfigure :tight @19:55:31.47 SC.home.seek :initial @19:55:33.70 PS.stop @19:56:01.61 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @19:56:01.64
Retry #1 of 3 @19:56:01.78 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @19:56:05.95 SC.configure :limp @19:56:06.05 Forearm.seek :retract @19:56:06.25 Elbow.seek :hammer @19:56:07.44 Forearm.reconfigure :tight @19:56:07.52 Forearm.seek retracted @19:56:08.04 PC.reconfigure :default @19:56:08.10
CC.reconfigure :default @19:56:08.28 CC.home.seek :open @19:56:08.32 PC.home.seek :open @19:56:12.20
Phase [initialPurge; da [100, 100] #da and wsr are run simultanelously] @19:56:12.22 Notice.email "At 19:56:12 began in tube 2: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_101322.rb:14\n{1 => 0,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:56:12, 24.5C, 16% humidity, 15.7psia, 14.021V, 0.263A, 0.263A avg, 3.69W",Subject: "Started DA_WCR_101322 phase initialPurge; da [100, 100] #da and wsr are run simultanelously" @19:56:12.26
Starting initialPurge @19:56:12.32 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:56:12.48 Hand.open @19:56:13.52 Elbow.reconfigure :tight @19:56:13.60 Elbow.seek FlushPuck::Garage @19:56:15.58 Elbow.reconfigure :loose @19:56:15.76 Forearm.reconfigure :loose @19:56:15.85 Forearm.seek :garage @19:56:17.08 Hand.close @19:56:17.97 Forearm.seek :retract @19:56:19.14 Elbow.reconfigure :tight @19:56:19.26 Elbow.seek CC @19:56:22.36 Elbow.reconfigure :loose @19:56:22.57 Forearm.reconfigure :tight @19:56:22.65 Forearm.seek CC @19:56:24.28 CC.seek :closed @19:56:28.71 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.85 @19:56:28.90 Hand.open @19:56:29.84 Forearm.reconfigure :loose @19:56:29.90 Forearm.seek :retract @19:56:30.94 Flushing the Collection Valve::Manifold @19:56:31.20 CSV.dial CSR @19:56:32.63 Pulling 5ml of CSR.flush @19:56:32.64 CSR.select :flush @19:56:32.81 CS.reconfigure :default @19:56:32.89 CS.seek 5ml @19:56:46.35 delay 5 seconds @19:56:51.38 CSR.select :air @19:56:51.63 CS.reconfigure :fast @19:56:51.77 CS.seek 7.5ml @19:56:58.18 CSV.dial :waste @19:56:59.44 CS.reconfigure :default @19:56:59.51 CS.seek 0ml @19:57:19.27 Purging external Intake @19:57:19.58 CSV.dial :flush @19:57:20.07 CS.reconfigure :fast @19:57:20.14 CS.seek 10ml @19:57:42.55 delay 5 seconds @19:57:47.59 CSV.dial CBV @19:57:47.65
CBV.dial CSV @19:57:48.46 CTV.dial :intake @19:57:49.17 Intake.select :open @19:57:49.30 CS.seek 0ml @19:58:11.78 Intake.select :close @19:58:11.89 CTV.dial :air @19:58:12.63 Flushing upper manifold @19:58:12.74 CSV.dial :flush @19:58:13.51 CS.seek 5ml @19:58:25.27 delay 5 seconds @19:58:30.31 CSV.dial :air @19:58:30.90 CS.seek 6.999ml @19:58:36.22 CSV.dial CBV @19:58:36.29
CBV.dial CSV @19:58:37.33 CTV.dial :air @19:58:37.37 CS.seek 0ml @19:58:53.39 delay 7 seconds @19:59:00.45 CS.seek 3ml @19:59:07.86 CSV.dial :waste @19:59:08.39 CS.seek 0ml @19:59:15.89 CSV.dial :flush @19:59:16.36 CS.seek 3ml @19:59:23.72 delay 5 seconds @19:59:28.73 CSV.dial :air @19:59:29.45 CS.seek 5ml @19:59:34.80 CSV.dial :air @19:59:34.84 CS.seek 0ml @19:59:46.50 delay 7 seconds @19:59:53.52 CS.seek 3ml @20:00:00.93 CSV.dial :waste @20:00:01.66 CS.seek 0ml @20:00:09.10 Garaging FlushPuck in Collection Clamp @20:00:09.24 Elbow.reconfigure :tight @20:00:09.32 Elbow.seek CC @20:00:09.49 Elbow.reconfigure :loose @20:00:09.58 Forearm.seek CC @20:00:10.62 Hand.close @20:00:11.46 CC.seek :open @20:00:16.00 Forearm.seek :retract @20:00:17.10 Elbow.reconfigure :tight @20:00:17.17 Elbow.seek :garage @20:00:20.28 Elbow.reconfigure :loose @20:00:20.46 Forearm.reconfigure :tight @20:00:20.54 Forearm.seek :garage @20:00:21.74 Hand.open @20:00:23.56 Forearm.reconfigure :loose @20:00:23.78 Forearm.seek :retract @20:00:24.95 da sampling at most 100ml, wcr at most 100ml @20:00:24.99 Loading sh1 puck from tube 2 into Collection Clamp @20:00:25.01 DAfans.email "Started da in tube 2 at 20:00:24UTC13-Oct-22\n{1 => 0,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:56:12, 24.5C, 16% humidity, 15.7psia, 14.021V, 0.263A, 0.263A avg, 3.69W",Subject: "da sampling at most 100ml, wcr at most 100ml" @20:00:25.51
SC.reconfigure :tight @20:00:25.59 SC.seek 2 @20:00:38.63 SC.configure :limp @20:00:39.10 Elbow.reconfigure :tight @20:00:39.19 Elbow.seek 2 @20:00:41.42 Forearm.reconfigure :tight @20:00:41.47 Forearm.seek 2 + 700 counts @20:00:42.58 SE.reconfigure :pick @20:00:42.70 SE.seek :beamBreak @20:00:44.32 SE.configure :default @20:00:44.44 SE.seek :down @20:00:45.63 SE.stop @20:00:45.83 Forearm.reconfigure :loose @20:00:45.91 Forearm.seek :retract @20:00:46.90 Puck::Error in MAIN -- 25 pucks loaded in tube 2 when expecting 33 @20:00:46.92 Waiting to Resume from Checkpoint "Loading sh1 puck from tube 2 into Collection Clamp" @20:00:46.95 BadNews.email "25 pucks loaded in tube 2 when expecting 33",Subject: "Puck::Error in MAIN" @20:05:09.56 Session Begun @20:05:30.62 -> abort! 'mission file incorrect' @20:05:30.64 CmdServer::Client::Error in dave -- Aborting Mission: mission file incorrect @20:05:30.74 BadNews.email "mission file incorrect",Subject: "DA_WCR_101322 ABORTED!" @20:05:46.09 -> exit @20:05:46.13 Session Ended @20:06:05.40
Uploading Logs @20:12:42.00UTC13-Oct-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @20:12:44.61 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @20:12:48.53 Polling Can every 10:00 @20:12:48.58 Loading /home/esp/esp2/mission/DA_WCR_101322.rb mission @20:12:48.63 GoodNews.email "Starting DA_WCR_101322 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@20:12:48, 24.6C, 16% humidity, 15.7psia, 14.055V, 0.233A, 0.233A avg, 3.27W",Subject: "DA_WCR_101322 begun" @20:12:48.65
Preparing to sleep until + 00:00:05 @20:12:48.70 Asleep til + 00:00:05 @20:12:48.72 Continuing at 20:12 @20:12:49.00 Gate.power :core,:OFF @20:12:49.01 Gate.power :raw,:OFF @20:12:49.02 Shutdown @20:12:53.51 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_101322.rb:14\n{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:12:48, 24.6C, 16% humidity, 15.7psia, 14.055V, 0.233A, 0.233A avg, 3.27W",Subject: "Asleep until 20:12" @20:12:53.68
Powering core bus @20:12:58.08 Net::SMTPUnknownError in Qmail -- could not get 3xx (421) @20:12:58.10 Email::Queue::Purge in Qmail -- Purged output queue @20:12:59.01 Gate.power :raw,:ON @20:13:00.50 CS.configure :default @20:13:00.69 PS.configure :default @20:13:00.88 SS.configure :default @20:13:01.11 CC.configure :default @20:13:01.29 PC.configure :default @20:13:01.51 Forearm.configure :loose @20:13:01.69 Elbow.configure :loose @20:13:01.91 SC.configure :loose @20:13:02.11 SE.configure :default @20:13:02.19 CH.coast @20:13:02.29 CH.configure :default @20:13:02.35 PH.coast @20:13:02.53 PH.configure :default @20:13:02.61 All dwarf controllers running firmware version 3.85 @20:13:02.75 Valve.reset @20:13:03.07 Intake.select :close @20:13:03.21 Exhaust.select :close @20:13:03.29 CTV.dial :air @20:13:03.53 PSV.dial :air @20:13:03.55 CSV.dial :waste1 @20:13:03.81 SSV.dial :spit,avoiding: :exhaust @20:13:03.95 PS.reconfigure :default @20:13:04.11
Forearm.reconfigure :loose @20:13:04.23 PS.home.seek 0ml @20:13:04.30
Forearm.home.seek :retract @20:13:04.62 CS.reconfigure :fast @20:13:04.66 SS.reconfigure :default @20:13:04.82 CS.home.seek 0ml @20:13:04.88 SS.home.seek 0ml @20:13:05.27 Hand.close @20:13:05.33
Elbow.jog 1500 @20:13:06.03 Elbow.reconfigure :loose @20:13:06.12 Elbow.home.seek :clear @20:13:10.69 SE.reconfigure :default @20:13:10.78 SE.home.seek :down @20:13:11.49 SC.reconfigure :tight @20:13:11.57 SC.home.seek :initial @20:13:13.01 PS.stop @20:13:41.66 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @20:13:41.69
Retry #1 of 3 @20:13:41.82 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @20:13:46.08 SC.configure :limp @20:13:46.17 Forearm.seek :retract @20:13:46.31 Elbow.seek :hammer @20:13:47.52 Forearm.reconfigure :tight @20:13:47.60 Forearm.seek retracted @20:13:48.04 PC.reconfigure :default @20:13:48.10
CC.reconfigure :default @20:13:48.35 CC.home.seek :open @20:13:48.39 PC.home.seek :open @20:13:52.29
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @20:13:52.31 Notice.email "At 20:13:52 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_101322.rb:14\n{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:13:52, 24.6C, 16% humidity, 15.7psia, 14.026V, 0.263A, 0.263A avg, 3.69W",Subject: "Started DA_WCR_101322 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @20:13:52.35
Starting initialPurge @20:13:52.41 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:13:52.55 Hand.open @20:13:53.54 Elbow.reconfigure :tight @20:13:53.62 Elbow.seek FlushPuck::Garage @20:13:55.72 Elbow.reconfigure :loose @20:13:55.93 Forearm.reconfigure :loose @20:13:55.99 Forearm.seek :garage @20:13:57.10 Hand.close @20:13:58.03 Forearm.seek :retract @20:13:59.16 Elbow.reconfigure :tight @20:13:59.24 Elbow.seek CC @20:14:02.20 Elbow.reconfigure :loose @20:14:02.46 Forearm.reconfigure :tight @20:14:02.54 Forearm.seek CC @20:14:04.16 CC.seek :closed @20:14:08.51 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.80 @20:14:08.70 Hand.open @20:14:09.56 Forearm.reconfigure :loose @20:14:09.64 Forearm.seek :retract @20:14:10.68 Flushing the Collection Valve::Manifold @20:14:10.91 CSV.dial CSR @20:14:12.42 Pulling 5ml of CSR.flush @20:14:12.43 CSR.select :flush @20:14:12.58 CS.reconfigure :default @20:14:12.66 CS.seek 5ml @20:14:26.14 delay 5 seconds @20:14:31.18 CSR.select :air @20:14:31.47 CS.reconfigure :fast @20:14:31.55 CS.seek 7.5ml @20:14:37.98 CSV.dial :waste @20:14:39.25 CS.reconfigure :default @20:14:39.34 CS.seek 0ml @20:14:41.96 upsync @20:14:59.09
Purging external Intake @20:14:59.20 CSV.dial :flush @20:14:59.64 CS.reconfigure :fast @20:14:59.68 CS.seek 10ml @20:15:22.24 delay 5 seconds @20:15:27.26 CSV.dial CBV @20:15:27.31
CBV.dial CSV @20:15:27.99 CTV.dial :intake @20:15:28.71 Intake.select :open @20:15:28.82 CS.seek 0ml @20:15:51.15 Intake.select :close @20:15:51.25 CTV.dial :air @20:15:51.98 Flushing upper manifold @20:15:52.09 CSV.dial :flush @20:15:52.81 CS.seek 5ml @20:16:04.37 delay 5 seconds @20:16:09.38 CSV.dial :air @20:16:09.88 CS.seek 6.999ml @20:16:15.09 CSV.dial CBV @20:16:15.11
CBV.dial CSV @20:16:16.23 CTV.dial :air @20:16:16.24 CS.seek 0ml @20:16:32.18 delay 7 seconds @20:16:39.20 CS.seek 3ml @20:16:46.55 CSV.dial :waste @20:16:46.98 CS.seek 0ml @20:16:54.44 CSV.dial :flush @20:16:54.87 CS.seek 3ml @20:17:02.15 delay 5 seconds @20:17:07.16 CSV.dial :air @20:17:07.66 CS.seek 5ml @20:17:12.89 CSV.dial :air @20:17:12.91 CS.seek 0ml @20:17:24.52 delay 7 seconds @20:17:31.55 CS.seek 3ml @20:17:38.99 CSV.dial :waste @20:17:39.70 CS.seek 0ml @20:17:47.02 Garaging FlushPuck in Collection Clamp @20:17:47.09 Elbow.reconfigure :tight @20:17:47.13 Elbow.seek CC @20:17:47.23 Elbow.reconfigure :loose @20:17:47.33 Forearm.seek CC @20:17:48.28 Hand.close @20:17:48.89 CC.seek :open @20:17:53.22 Forearm.seek :retract @20:17:54.30 Elbow.reconfigure :tight @20:17:54.34 Elbow.seek :garage @20:17:57.43 Elbow.reconfigure :loose @20:17:57.52 Forearm.reconfigure :tight @20:17:57.56 Forearm.seek :garage @20:17:58.64 Hand.open @20:17:59.79 Forearm.reconfigure :loose @20:17:59.90 Forearm.seek :retract @20:18:00.98 da sampling at most 1000ml, wcr at most 1000ml @20:18:01.02 Loading sh1 puck from tube 2 into Collection Clamp @20:18:01.03 DAfans.email "Started da in tube 2 at 20:18:00UTC13-Oct-22\n{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:13:52, 24.6C, 16% humidity, 15.7psia, 14.026V, 0.263A, 0.263A avg, 3.69W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @20:18:01.28
SC.reconfigure :tight @20:18:01.32 SC.seek 2 @20:18:14.30 SC.configure :limp @20:18:14.81 Elbow.reconfigure :tight @20:18:14.93 Elbow.seek 2 @20:18:17.00 Forearm.reconfigure :tight @20:18:17.10 Forearm.seek 2 + 700 counts @20:18:18.36 SE.reconfigure :pick @20:18:18.46 SE.seek :beamBreak @20:18:20.05 SE.configure :default @20:18:20.39 Elbow.seek 2 @20:18:20.78 Forearm.seek 2 + 300 counts @20:18:21.03 Forearm.reconfigure :loose @20:18:21.11 Hand.close @20:18:21.82 SE.seek :down @20:18:22.20
Forearm.seek :retract @20:18:23.16 SE.stop @20:18:23.30
Elbow.seek CC @20:18:25.02 Elbow.reconfigure :loose @20:18:25.23 Forearm.reconfigure :tight @20:18:25.30 Forearm.seek CC @20:18:27.02 CC.seek :closed @20:18:31.37 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.73 @20:18:31.39 Hand.open @20:18:32.29 Forearm.reconfigure :loose @20:18:32.35 Forearm.seek :retract @20:18:33.44 collectSample 1000ml w/bubble point of 24psi @20:18:33.46 Priming 7ml @20:18:33.48 Intake.select :close @20:18:33.50 Exhaust.select :close @20:18:33.52 SS.hold @20:18:33.91 Calibrating pressure and temperature sensors @20:18:33.97 CTV.dial :air @20:18:33.99 SSV.dial :spit,avoiding: :exhaust @20:18:34.03 SS.reconfigure :fast @20:18:34.11 SS.seek 5ml @20:18:37.17 SSV.dial CBV @20:18:37.25
CBV.dial SSV @20:18:38.54 delay 3.8 seconds @20:18:42.57 SSV.dial :spit @20:18:43.91 SS.seek 0ml @20:18:46.70 SSV.dial CBV @20:18:48.00 CTV.dial :intake @20:18:48.74 SSV.dial CBV @20:18:48.81
CBV.dial SSV @20:18:48.85 Allowing 40s for 7.0ml of remaining 7.0ml @20:18:48.86 Intake.select :open @20:18:51.18 Intake @15.1psia, vacuum from 9.6 to 12.6psi @20:18:51.21 SS.reconfigure :inhaling 2.14ml/s @20:18:51.31 SS.seek 7ml @20:18:58.33 Stroke completed -- waiting for pressure to equalize ... @20:18:58.35 SS.reconfigure :exhale @20:18:58.41 SS.hold @20:19:01.99 Intake.select :close @20:19:02.21 SSV.dial :exhaust @20:19:02.97 Exhaust.select :open @20:19:03.13 SS.seek 0ml @20:19:07.06 Exhaust.select :close @20:19:07.25 Sampling 1000.0ml @20:19:07.27 Intake.select :close @20:19:07.29 Exhaust.select :close @20:19:07.31 SS.reconfigure :default @20:19:07.39 SS.hold @20:19:07.78 CTV.dial :intake @20:19:07.80 SSV.dial CBV @20:19:07.86
CBV.dial SSV @20:19:08.66 Allowing 130s for 25.0ml of remaining 1000.0ml @20:19:08.68 Intake.select :open @20:19:10.93 Intake @15.2psia, vacuum from 9.7 to 12.7psi @20:19:10.96 SS.reconfigure :inhaling 2.14ml/s @20:19:11.03 SS.seek 25ml @20:19:17.12 Vacuum too high -- waiting up to 40 seconds @20:19:17.14 SS.reconfigure :exhale @20:19:17.21 SS.hold @20:19:17.96 Intake @12.0psia, vacuum from 6.5 to 9.5psi @20:19:17.99 SS.reconfigure :inhaling 1.93ml/s @20:19:18.08 SS.seek 25ml @20:19:20.88 Vacuum too high -- waiting up to 40 seconds @20:19:20.90 SS.reconfigure :exhale @20:19:21.99 SS.hold @20:19:22.49 Intake @15.1psia, vacuum from 9.6 to 12.6psi @20:19:22.53 SS.reconfigure :inhaling 1.74ml/s @20:19:22.61 SS.seek 25ml @20:19:25.57 Vacuum too high -- waiting up to 40 seconds @20:19:25.59 SS.reconfigure :exhale @20:19:25.67 SS.hold @20:19:26.38 Intake @13.1psia, vacuum from 7.6 to 10.6psi @20:19:26.42 SS.reconfigure :inhaling 1.57ml/s @20:19:26.52 SS.seek 25ml @20:19:29.65 Vacuum too high -- waiting up to 40 seconds @20:19:29.67 SS.reconfigure :exhale @20:19:29.73 SS.hold @20:19:31.02 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:19:31.06 SS.reconfigure :inhaling 1.41ml/s @20:19:31.14 SS.seek 25ml @20:19:34.40 Vacuum too high -- waiting up to 40 seconds @20:19:34.42 SS.reconfigure :exhale @20:19:34.50 SS.hold @20:19:35.41 Intake @13.9psia, vacuum from 8.4 to 11.4psi @20:19:35.45 SS.reconfigure :inhaling 1.27ml/s @20:19:35.53 SS.seek 25ml @20:19:39.93 Vacuum too high -- waiting up to 40 seconds @20:19:39.95 SS.reconfigure :exhale @20:19:40.10 SS.hold @20:19:40.86 Intake @12.9psia, vacuum from 7.4 to 10.4psi @20:19:40.89 SS.reconfigure :inhaling 1.14ml/s @20:19:40.97 SS.seek 25ml @20:19:44.48 Stroke completed -- waiting for pressure to equalize ... @20:19:44.50 SS.reconfigure :exhale @20:19:44.58 SS.hold @20:19:46.09 Intake.select :close @20:19:46.30 SSV.dial :exhaust @20:19:47.09 Exhaust.select :open @20:19:47.25 SS.seek 0ml @20:19:55.42 upsync @20:19:56.37
Exhaust.select :close @20:19:56.47 SSV.dial CBV @20:19:56.51
CBV.dial SSV @20:19:57.23 Allowing 130s for 25.0ml of remaining 975.0ml @20:19:57.24 Intake.select :open @20:19:59.40 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:19:59.41 SS.reconfigure :inhaling 1.14ml/s @20:19:59.45 SS.seek 25ml @20:20:07.47 Vacuum too high -- waiting up to 40 seconds @20:20:07.48 SS.reconfigure :exhale @20:20:07.52 SS.hold @20:20:08.21 Intake @13.3psia, vacuum from 7.8 to 10.8psi @20:20:08.22 SS.reconfigure :inhaling 1.03ml/s @20:20:08.27 SS.seek 25ml @20:20:12.38 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @20:20:12.42 SS.hold @20:20:13.10 Intake @13.9psia, vacuum from 8.4 to 11.4psi @20:20:13.12 SS.reconfigure :inhaling 0.93ml/s @20:20:13.16 SS.seek 25ml @20:20:29.68 Stroke completed -- waiting for pressure to equalize ... @20:20:29.69 SS.reconfigure :exhale @20:20:29.73 SS.hold @20:20:31.27 Intake.select :close @20:20:31.41 SSV.dial :exhaust @20:20:32.13 Exhaust.select :open @20:20:32.24 SS.seek 0ml @20:20:41.49 Exhaust.select :close @20:20:41.59 SSV.dial CBV @20:20:41.62
CBV.dial SSV @20:20:42.35 Allowing 130s for 25.0ml of remaining 950.0ml @20:20:42.36 Intake.select :open @20:20:44.52 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:20:44.53 SS.reconfigure :inhaling 0.93ml/s @20:20:44.57 SS.seek 25ml @20:21:13.66 Stroke completed -- waiting for pressure to equalize ... @20:21:13.67 SS.reconfigure :exhale @20:21:13.71 SS.hold @20:21:16.49 Intake.select :close @20:21:16.63 SSV.dial :exhaust @20:21:17.33 Exhaust.select :open @20:21:17.44 SS.seek 0ml @20:21:26.35 Exhaust.select :close @20:21:26.45 SSV.dial CBV @20:21:26.50
CBV.dial SSV @20:21:27.19 Allowing 130s for 25.0ml of remaining 925.0ml @20:21:27.20 Intake.select :open @20:21:29.35 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:21:29.37 SS.reconfigure :inhaling 0.93ml/s @20:21:29.41 SS.seek 25ml @20:21:59.22 Stroke completed -- waiting for pressure to equalize ... @20:21:59.23 SS.reconfigure :exhale @20:21:59.27 SS.hold @20:22:01.21 Intake.select :close @20:22:01.35 SSV.dial :exhaust @20:22:02.08 Exhaust.select :open @20:22:02.19 SS.seek 0ml @20:22:11.47 Exhaust.select :close @20:22:11.57 SSV.dial CBV @20:22:11.60
CBV.dial SSV @20:22:12.35 Allowing 130s for 25.0ml of remaining 900.0ml @20:22:12.36 Intake.select :open @20:22:14.51 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:22:14.53 SS.reconfigure :inhaling 0.93ml/s @20:22:14.57 SS.seek 25ml @20:22:43.30 Stroke completed -- waiting for pressure to equalize ... @20:22:43.31 SS.reconfigure :exhale @20:22:43.35 SS.hold @20:22:45.30 Intake.select :close @20:22:45.44 SSV.dial :exhaust @20:22:46.16 Exhaust.select :open @20:22:46.27 SS.seek 0ml @20:22:55.45 Exhaust.select :close @20:22:55.56 SSV.dial CBV @20:22:55.59
CBV.dial SSV @20:22:56.32 Allowing 130s for 25.0ml of remaining 875.0ml @20:22:56.33 Intake.select :open @20:22:58.96 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:22:58.97 SS.reconfigure :inhaling 0.93ml/s @20:22:59.02 SS.seek 25ml @20:23:27.83 Stroke completed -- waiting for pressure to equalize ... @20:23:27.84 SS.reconfigure :exhale @20:23:27.88 SS.hold @20:23:29.82 Intake.select :close @20:23:29.96 SSV.dial :exhaust @20:23:30.67 Exhaust.select :open @20:23:30.78 SS.seek 0ml @20:23:40.09 Exhaust.select :close @20:23:40.20 SSV.dial CBV @20:23:40.22
CBV.dial SSV @20:23:40.96 Allowing 130s for 25.0ml of remaining 850.0ml @20:23:40.97 Intake.select :open @20:23:43.12 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:23:43.14 SS.reconfigure :inhaling 0.93ml/s @20:23:43.18 SS.seek 25ml @20:24:11.79 Stroke completed -- waiting for pressure to equalize ... @20:24:11.80 SS.reconfigure :exhale @20:24:11.85 SS.hold @20:24:13.40 Intake.select :close @20:24:13.54 SSV.dial :exhaust @20:24:14.27 Exhaust.select :open @20:24:14.37 SS.seek 0ml @20:24:23.28 Exhaust.select :close @20:24:23.38 SSV.dial CBV @20:24:23.41
CBV.dial SSV @20:24:24.16 Allowing 130s for 25.0ml of remaining 825.0ml @20:24:24.17 Intake.select :open @20:24:26.32 Intake @14.2psia, vacuum from 8.7 to 11.7psi @20:24:26.34 SS.reconfigure :inhaling 0.93ml/s @20:24:26.38 SS.seek 25ml @20:24:55.37 Stroke completed -- waiting for pressure to equalize ... @20:24:55.38 SS.reconfigure :exhale @20:24:55.42 SS.hold @20:24:56.97 Intake.select :close @20:24:57.10 SSV.dial :exhaust @20:24:57.83 Exhaust.select :open @20:24:57.93 SS.seek 0ml @20:25:07.32 Exhaust.select :close @20:25:07.43 SSV.dial CBV @20:25:07.45
CBV.dial SSV @20:25:08.19 Allowing 130s for 25.0ml of remaining 800.0ml @20:25:08.20 Intake.select :open @20:25:10.35 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:25:10.36 SS.reconfigure :inhaling 0.93ml/s @20:25:10.41 SS.seek 25ml @20:25:39.43 Stroke completed -- waiting for pressure to equalize ... @20:25:39.44 SS.reconfigure :exhale @20:25:39.48 SS.hold @20:25:41.87 Intake.select :close @20:25:42.01 SSV.dial :exhaust @20:25:42.73 Exhaust.select :open @20:25:42.84 SS.seek 0ml @20:25:51.82 Exhaust.select :close @20:25:51.93 SSV.dial CBV @20:25:51.95
CBV.dial SSV @20:25:52.69 Allowing 130s for 25.0ml of remaining 775.0ml @20:25:52.70 Intake.select :open @20:25:54.85 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:25:54.86 SS.reconfigure :inhaling 0.93ml/s @20:25:54.91 SS.seek 25ml @20:26:24.67 Stroke completed -- waiting for pressure to equalize ... @20:26:24.68 SS.reconfigure :exhale @20:26:24.72 SS.hold @20:26:26.67 Intake.select :close @20:26:26.80 SSV.dial :exhaust @20:26:27.51 Exhaust.select :open @20:26:27.62 SS.seek 0ml @20:26:36.54 Exhaust.select :close @20:26:36.64 SSV.dial CBV @20:26:36.67
CBV.dial SSV @20:26:37.40 Allowing 130s for 25.0ml of remaining 750.0ml @20:26:37.41 Intake.select :open @20:26:39.56 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:26:39.58 SS.reconfigure :inhaling 0.93ml/s @20:26:39.62 SS.seek 25ml @20:27:08.60 Stroke completed -- waiting for pressure to equalize ... @20:27:08.61 SS.reconfigure :exhale @20:27:08.65 SS.hold @20:27:11.04 Intake.select :close @20:27:11.18 SSV.dial :exhaust @20:27:11.90 Exhaust.select :open @20:27:12.01 SS.seek 0ml @20:27:21.26 Exhaust.select :close @20:27:21.36 SSV.dial CBV @20:27:21.41
CBV.dial SSV @20:27:22.10 Allowing 130s for 25.0ml of remaining 725.0ml @20:27:22.11 Intake.select :open @20:27:24.28 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:27:24.30 SS.reconfigure :inhaling 0.93ml/s @20:27:24.34 SS.seek 25ml @20:27:53.15 Stroke completed -- waiting for pressure to equalize ... @20:27:53.16 SS.reconfigure :exhale @20:27:53.20 SS.hold @20:27:55.23 Intake.select :close @20:27:55.37 SSV.dial :exhaust @20:27:56.09 Exhaust.select :open @20:27:56.19 SS.seek 0ml @20:28:05.40 Exhaust.select :close @20:28:05.50 SSV.dial CBV @20:28:05.55
CBV.dial SSV @20:28:06.24 Allowing 130s for 25.0ml of remaining 700.0ml @20:28:06.25 Intake.select :open @20:28:08.41 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:28:08.42 SS.reconfigure :inhaling 0.93ml/s @20:28:08.46 SS.seek 25ml @20:28:37.18 Stroke completed -- waiting for pressure to equalize ... @20:28:37.19 SS.reconfigure :exhale @20:28:37.61 SS.hold @20:28:39.34 Intake.select :close @20:28:39.48 SSV.dial :exhaust @20:28:40.21 Exhaust.select :open @20:28:40.32 SS.seek 0ml @20:28:49.74 Exhaust.select :close @20:28:49.84 SSV.dial CBV @20:28:49.87
CBV.dial SSV @20:28:50.60 Allowing 130s for 25.0ml of remaining 675.0ml @20:28:50.61 Intake.select :open @20:28:52.76 Intake @14.4psia, vacuum from 8.9 to 11.9psi @20:28:52.78 SS.reconfigure :inhaling 0.93ml/s @20:28:52.82 SS.seek 25ml @20:29:21.66 Stroke completed -- waiting for pressure to equalize ... @20:29:21.67 SS.reconfigure :exhale @20:29:21.71 SS.hold @20:29:24.11 Intake.select :close @20:29:24.25 SSV.dial :exhaust @20:29:24.96 Exhaust.select :open @20:29:25.07 SS.seek 0ml @20:29:34.36 Exhaust.select :close @20:29:34.47 SSV.dial CBV @20:29:34.49
CBV.dial SSV @20:29:35.23 Allowing 130s for 25.0ml of remaining 650.0ml @20:29:35.24 Intake.select :open @20:29:37.39 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:29:37.41 SS.reconfigure :inhaling 0.93ml/s @20:29:37.45 SS.seek 25ml @20:29:56.07 Vacuum too high -- waiting up to 40 seconds @20:29:56.08 SS.reconfigure :exhale @20:29:56.12 SS.hold @20:29:56.80 Intake @12.6psia, vacuum from 7.1 to 10.1psi @20:29:56.81 SS.reconfigure :inhaling 0.84ml/s @20:29:56.86 SS.seek 25ml @20:30:08.25 Stroke completed -- waiting for pressure to equalize ... @20:30:08.26 SS.reconfigure :exhale @20:30:08.30 SS.hold @20:30:10.25 Intake.select :close @20:30:10.40 SSV.dial :exhaust @20:30:11.13 Exhaust.select :open @20:30:11.24 SS.seek 0ml @20:30:20.20 Exhaust.select :close @20:30:20.31 SSV.dial CBV @20:30:20.34
CBV.dial SSV @20:30:21.08 Allowing 130s for 25.0ml of remaining 625.0ml @20:30:21.09 Intake.select :open @20:30:23.25 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:30:23.26 SS.reconfigure :inhaling 0.84ml/s @20:30:23.31 SS.seek 25ml @20:30:55.72 Stroke completed -- waiting for pressure to equalize ... @20:30:55.73 SS.reconfigure :exhale @20:30:55.77 SS.hold @20:30:57.85 Intake.select :close @20:30:57.99 SSV.dial :exhaust @20:30:58.72 Exhaust.select :open @20:30:58.83 SS.seek 0ml @20:31:08.13 Exhaust.select :close @20:31:08.23 SSV.dial CBV @20:31:08.26
CBV.dial SSV @20:31:08.99 Allowing 130s for 25.0ml of remaining 600.0ml @20:31:09.00 Intake.select :open @20:31:11.15 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:31:11.16 SS.reconfigure :inhaling 0.84ml/s @20:31:11.21 SS.seek 25ml @20:31:42.61 Stroke completed -- waiting for pressure to equalize ... @20:31:42.62 SS.reconfigure :exhale @20:31:42.66 SS.hold @20:31:46.10 Intake.select :close @20:31:46.23 SSV.dial :exhaust @20:31:46.94 Exhaust.select :open @20:31:47.05 SS.seek 0ml @20:31:56.00 Exhaust.select :close @20:31:56.10 SSV.dial CBV @20:31:56.13
CBV.dial SSV @20:31:56.86 Allowing 130s for 25.0ml of remaining 575.0ml @20:31:56.87 Intake.select :open @20:31:59.02 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:31:59.04 SS.reconfigure :inhaling 0.84ml/s @20:31:59.08 SS.seek 25ml @20:32:11.66 Vacuum too high -- waiting up to 40 seconds @20:32:11.67 SS.reconfigure :exhale @20:32:11.71 SS.hold @20:32:12.98 Intake @13.2psia, vacuum from 7.7 to 10.7psi @20:32:13.00 SS.reconfigure :inhaling 0.76ml/s @20:32:13.04 SS.seek 25ml @20:32:25.22 Vacuum too high -- waiting up to 40 seconds @20:32:25.23 SS.reconfigure :exhale @20:32:25.28 SS.hold @20:32:27.93 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:32:27.95 SS.reconfigure :inhaling 0.69ml/s @20:32:27.99 SS.seek 25ml @20:32:40.81 Stroke completed -- waiting for pressure to equalize ... @20:32:40.82 SS.reconfigure :exhale @20:32:40.86 SS.hold @20:32:45.30 Intake.select :close @20:32:45.44 SSV.dial :exhaust @20:32:46.17 Exhaust.select :open @20:32:46.28 SS.seek 0ml @20:32:55.68 Exhaust.select :close @20:32:55.79 SSV.dial CBV @20:32:55.82
CBV.dial SSV @20:32:56.57 Allowing 130s for 25.0ml of remaining 550.0ml @20:32:56.58 Intake.select :open @20:32:58.73 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:32:58.75 SS.reconfigure :inhaling 0.69ml/s @20:32:58.79 SS.seek 25ml @20:33:11.35 Vacuum too high -- waiting up to 40 seconds @20:33:11.36 SS.reconfigure :exhale @20:33:11.40 SS.hold @20:33:13.88 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:33:13.90 SS.reconfigure :inhaling 0.61ml/s @20:33:13.94 SS.seek 25ml @20:33:26.50 Vacuum too high -- waiting up to 40 seconds @20:33:26.51 SS.reconfigure :exhale @20:33:26.56 SS.hold @20:33:29.05 SS.reconfigure :inhaling 0.56ml/s @20:33:29.09 SS.seek 25ml @20:33:48.04 Stroke completed -- waiting for pressure to equalize ... @20:33:48.05 SS.reconfigure :exhale @20:33:48.09 SS.hold @20:33:55.57 Intake.select :close @20:33:55.71 SSV.dial :exhaust @20:33:56.43 Exhaust.select :open @20:33:56.54 SS.seek 0ml @20:34:05.81 Exhaust.select :close @20:34:05.91 SSV.dial CBV @20:34:05.96
CBV.dial SSV @20:34:06.65 Allowing 130s for 25.0ml of remaining 525.0ml @20:34:06.66 Intake.select :open @20:34:08.82 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:34:08.83 SS.reconfigure :inhaling 0.56ml/s @20:34:08.88 SS.seek 25ml @20:34:17.85 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @20:34:17.90 SS.hold @20:34:20.38 Intake @14.1psia, vacuum from 8.6 to 11.6psi @20:34:20.40 SS.reconfigure :inhaling 0.50ml/s @20:34:20.44 SS.seek 25ml @20:34:30.81 Vacuum too high -- waiting up to 40 seconds @20:34:30.82 SS.reconfigure :exhale @20:34:30.86 SS.hold @20:34:33.24 Intake @13.6psia, vacuum from 8.1 to 11.1psi @20:34:33.26 SS.reconfigure :inhaling 0.46ml/s @20:34:33.30 SS.seek 25ml @20:34:59.69 Vacuum too high -- waiting up to 40 seconds @20:34:59.70 SS.reconfigure :exhale @20:34:59.74 SS.hold @20:35:03.92 Intake @14.0psia, vacuum from 8.5 to 11.5psi @20:35:03.93 SS.reconfigure :inhaling 0.41ml/s @20:35:03.98 SS.seek 25ml @20:35:17.80 Stroke completed -- waiting for pressure to equalize ... @20:35:17.81 SS.reconfigure :exhale @20:35:17.86 SS.hold @20:35:25.31 Intake.select :close @20:35:25.45 SSV.dial :exhaust @20:35:26.16 Exhaust.select :open @20:35:26.27 SS.seek 0ml @20:35:35.62 Exhaust.select :close @20:35:35.72 SSV.dial CBV @20:35:35.77
CBV.dial SSV @20:35:36.46 Allowing 130s for 25.0ml of remaining 500.0ml @20:35:36.47 Intake.select :open @20:35:38.63 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:35:38.65 SS.reconfigure :inhaling 0.41ml/s @20:35:38.69 SS.seek 25ml @20:35:50.84 Vacuum too high -- waiting up to 40 seconds @20:35:50.85 SS.reconfigure :exhale @20:35:50.89 SS.hold @20:35:54.94 Intake @13.9psia, vacuum from 8.4 to 11.4psi @20:35:54.96 SS.reconfigure :inhaling 0.37ml/s @20:35:55.00 SS.seek 25ml @20:36:08.09 Vacuum too high -- waiting up to 40 seconds @20:36:08.10 SS.reconfigure :exhale @20:36:08.15 SS.hold @20:36:13.78 Intake @14.2psia, vacuum from 8.7 to 11.7psi @20:36:13.80 SS.reconfigure :inhaling 0.33ml/s @20:36:13.84 SS.seek 25ml @20:36:31.00 Vacuum too high -- waiting up to 40 seconds @20:36:31.01 SS.reconfigure :exhale @20:36:31.05 SS.hold @20:36:35.79 Intake @14.1psia, vacuum from 8.6 to 11.6psi @20:36:35.80 SS.reconfigure :inhaling 0.30ml/s @20:36:35.84 SS.seek 25ml @20:37:02.71 Vacuum too high -- waiting up to 40 seconds @20:37:02.72 SS.reconfigure :exhale @20:37:02.77 SS.hold @20:37:09.29 Intake @13.9psia, vacuum from 8.4 to 11.4psi @20:37:09.31 SS.reconfigure :inhaling 0.27ml/s @20:37:09.35 SS.seek 25ml @20:37:30.33 Stroke completed -- waiting for pressure to equalize ... @20:37:30.34 SS.reconfigure :exhale @20:37:30.38 SS.hold @20:37:37.73 Intake.select :close @20:37:37.87 SSV.dial :exhaust @20:37:38.60 Exhaust.select :open @20:37:38.71 SS.seek 0ml @20:37:47.69 Exhaust.select :close @20:37:47.80 SSV.dial CBV @20:37:47.82
CBV.dial SSV @20:37:48.55 Allowing 130s for 25.0ml of remaining 475.0ml @20:37:48.56 Intake.select :open @20:37:50.72 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:37:50.73 SS.reconfigure :inhaling 0.27ml/s @20:37:50.78 SS.seek 25ml @20:38:49.90 Vacuum too high -- waiting up to 40 seconds @20:38:49.91 SS.reconfigure :exhale @20:38:49.95 SS.hold @20:38:55.47 Intake @14.4psia, vacuum from 8.9 to 11.9psi @20:38:55.49 SS.reconfigure :inhaling 0.24ml/s @20:38:55.53 SS.seek 25ml @20:39:49.84 Stroke completed -- waiting for pressure to equalize ... @20:39:49.85 SS.reconfigure :exhale @20:39:49.89 SS.hold @20:39:58.33 Intake.select :close @20:39:58.47 SSV.dial :exhaust @20:39:59.17 Exhaust.select :open @20:39:59.28 SS.seek 0ml @20:40:08.62 Exhaust.select :close @20:40:08.73 SSV.dial CBV @20:40:08.75
CBV.dial SSV @20:40:09.48 Allowing 130s for 25.0ml of remaining 450.0ml @20:40:09.50 Intake.select :open @20:40:11.65 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:40:11.66 SS.reconfigure :inhaling 0.24ml/s @20:40:11.71 SS.seek 25ml @20:42:10.65 Stroke completed -- waiting for pressure to equalize ... @20:42:10.66 SS.reconfigure :exhale @20:42:10.70 SS.hold @20:42:19.50 SS.reconfigure :default @20:42:19.54 SS.hold @20:42:19.65 Intake.select :close @20:42:19.78 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 575.0ml @20:42:19.80 SSV.dial :exhaust @20:42:20.53 Exhaust.select :open @20:42:20.63 SS.reconfigure :exhale @20:42:20.67 SS.seek 0ml @20:42:29.84 Exhaust.select :close @20:42:29.94 Sampled 575.0ml @20:42:29.95 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @20:42:29.96 Intake.select :close @20:42:30.02 CSV.dial :waste @20:42:30.08 CS.seek 0ml @20:42:30.15 CSV.dial :air @20:42:30.20 SSV.dial CBV,avoiding: :exhaust @20:42:30.23
CBV.dial SSV @20:42:30.89 CS.seek 10ml @20:42:32.05
CTV.dial :air @20:42:32.76 delay 15 seconds @20:42:47.78 SSV.dial :spit,avoiding: :exhaust @20:42:49.08 CTV.dial CSV,avoiding: :intake @20:42:49.12
SS.seek 0ml @20:42:49.23 SSV.dial CBV,avoiding: :exhaust @20:42:53.54 CBV.dial between SSV and waste2 @20:42:53.91 CTV.dial CSV,avoiding: :air @20:42:53.94
CSV.dial CTV @20:42:54.37 CS.reconfigure :pressurize @20:42:54.41 CS.seek 0ml @20:43:15.73 BubblePoint: Intake @40.0psia, Outlet @15.9psia @20:43:15.74 CBV.dial SSV @20:43:16.06 SS.reconfigure :default @20:43:16.10 SS.seek 10ml @20:43:21.54 delay 15 seconds @20:43:36.65 Evacuated: Intake @17.6psia, Outlet @11.1psia @20:43:36.67 SSV.dial :spit,avoiding: :exhaust @20:43:37.97 SS.reconfigure :exhale @20:43:38.01 SS.seek 0ml @20:43:43.36 SS.reconfigure :fast @20:43:43.40 SS.seek 5ml @20:43:46.43 SSV.dial CBV,avoiding: :exhaust @20:43:46.46
CBV.dial SSV @20:43:47.76 CTV.dial :air,avoiding: :intake @20:43:49.03 CSV.dial CTV @20:43:49.06
SS.seek 10ml @20:43:50.06 CSV.dial :air @20:43:50.51 CS.reconfigure :fast @20:43:50.56 CS.seek 0ml @20:43:51.71
SSV.dial :spit,avoiding: :exhaust @20:43:53.01 SS.reconfigure :exhale @20:43:53.06 SS.seek 0ml @20:43:55.63 CS.stop @20:43:58.70
SS.reconfigure :fast @20:43:58.75 SS.seek 5ml @20:44:02.09 SSV.dial CBV,avoiding: :exhaust @20:44:02.12
CBV.dial SSV @20:44:04.42 SSV.dial :spit,avoiding: :exhaust @20:44:05.72 SS.reconfigure :default @20:44:05.76 SS.seek 0ml @20:44:09.11 SS.stop @20:44:09.15 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @20:44:09.16 Intake.select :close @20:44:09.23 CSV.dial :waste @20:44:09.34 SSV.dial CBV,avoiding: :exhaust @20:44:09.37
CBV.dial SSV @20:44:09.98 CS.seek 0ml @20:44:10.04 CSV.dial :air @20:44:10.65
CTV.dial :air @20:44:10.66 delay 15 seconds @20:44:10.76 CS.seek 10ml @20:44:25.68
SSV.dial :spit,avoiding: :exhaust @20:44:26.96 CTV.dial CSV,avoiding: :intake @20:44:27.00
SS.reconfigure :exhale @20:44:27.09 SS.seek 0ml @20:44:27.63 SSV.dial CBV,avoiding: :exhaust @20:44:33.19 CBV.dial between SSV and waste2 @20:44:33.57 CTV.dial CSV,avoiding: :air @20:44:33.59
CSV.dial CTV @20:44:34.14 CS.reconfigure :pressurize @20:44:34.18 CS.seek 0ml @20:44:55.50 BubblePoint: Intake @39.1psia, Outlet @15.0psia @20:44:55.51 CBV.dial SSV @20:44:55.84 SS.reconfigure :default @20:44:55.88 SS.seek 10ml @20:45:01.51 delay 15 seconds @20:45:16.62 Evacuated: Intake @18.1psia, Outlet @11.6psia @20:45:16.63 SSV.dial :spit,avoiding: :exhaust @20:45:17.92 SS.reconfigure :exhale @20:45:17.96 SS.seek 0ml @20:45:23.39 SS.reconfigure :fast @20:45:23.43 SS.seek 5ml @20:45:26.26 SSV.dial CBV,avoiding: :exhaust @20:45:26.30
CBV.dial SSV @20:45:27.56 CTV.dial :air,avoiding: :intake @20:45:28.80 CSV.dial CTV @20:45:28.84
SS.seek 10ml @20:45:29.84 CSV.dial :air @20:45:30.26 CS.reconfigure :fast @20:45:30.30 CS.seek 0ml @20:45:31.27
SSV.dial :spit,avoiding: :exhaust @20:45:32.56 SS.reconfigure :exhale @20:45:32.60 SS.seek 0ml @20:45:35.00 CS.stop @20:45:37.89
SS.reconfigure :fast @20:45:37.93 SS.seek 5ml @20:45:40.73 SSV.dial CBV,avoiding: :exhaust @20:45:40.76
CBV.dial SSV @20:45:43.05 SSV.dial :spit,avoiding: :exhaust @20:45:44.32 SS.reconfigure :default @20:45:44.36 SS.seek 0ml @20:45:47.70 SS.stop @20:45:47.74 Lysing: Adding 2.0ml AqMeOH @20:45:47.75 CBV.dial between CSV and waste1,avoiding: :DBV @20:45:49.18 CTV.dial CSV @20:45:50.44 CSV.dial CSR @20:45:51.20 Pulling 1ml of CSR.AqMeOH @20:45:51.21 CSR.select :AqMeOH @20:45:51.31 CS.reconfigure :default @20:45:51.35 CS.seek 1ml @20:45:55.13 delay 5 seconds @20:46:00.15 CSR.select :air @20:46:00.30 CS.seek 6ml @20:46:13.75 delay 2 seconds @20:46:15.77 CSV.dial :waste2 @20:46:16.21 CS.reconfigure :fast @20:46:16.25 CS.seek 0ml @20:46:30.06 CSV.dial CSR @20:46:30.56 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @20:46:30.67 CS.reconfigure :default @20:46:30.71 CS.seek 2ml @20:46:36.63 delay 5 seconds @20:46:41.65 CSR.select :air @20:46:41.80 CS.seek 5ml @20:46:50.16 delay 2 seconds @20:46:52.17 CSV.dial CTV @20:46:52.60 CS.seek 1ml @20:47:03.57 Before lysing Collection Puck Heater at 19.9C/38.4psia @20:47:03.59 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @20:47:03.61 CH.seek 70C,8:00 @20:48:50.46 CH.hold 10:00,70C @20:48:50.63 Begun lysing Collection Puck Heater at 68.1C/38.2psia @20:58:50.65 Ended lysing Collection Puck Heater at 70C/36.8psia @20:58:50.66 CS.seek 0ml @20:58:54.08 CTV.dial between spare and air,avoiding: :intake @20:58:55.06 CSV.dial :air @20:58:55.50 CS.seek 5ml @20:59:08.95 CSV.dial CTV @20:59:09.50 delay 2 seconds @20:59:11.51 CSV.dial :air @20:59:11.93 delay 2 seconds @20:59:13.95 CSV.dial CTV @20:59:14.39 delay 2 seconds @20:59:16.40 CSV.dial :air @20:59:16.97 delay 2 seconds @20:59:18.99 CS.seek 0ml @20:59:32.41 Puck @46.7psia after final lysis pressurization @20:59:32.42 Recovering Lysate into Collection syringe @20:59:32.43 CSV.dial CBV @20:59:33.54 CBV.dial CSV @20:59:33.90 CS.seek 5ml @20:59:47.32 delay 5 seconds @20:59:52.34 CTV.dial :air @20:59:52.85 CS.seek 6.999ml @20:59:58.67 delay 5 seconds @21:00:03.68 CSV.dial :air2 @21:00:04.14 CS.seek 10ml @21:00:12.48 CSV.dial between air and CTV,avoiding: CBV @21:00:13.59 Lysing Complete @21:00:13.66 Storing sh1 puck in Collection Clamp to tube 1 @21:00:13.82 SC.reconfigure :tight @21:00:13.86 SC.seek 1 @21:00:17.97 SC.configure :limp @21:00:18.16 Elbow.reconfigure :tight @21:00:18.20 Elbow.seek 1 @21:00:19.87 Forearm.reconfigure :tight @21:00:19.91 Forearm.seek 1 + 700 counts @21:00:21.00 SE.reconfigure :pick @21:00:21.05 SE.seek :beamBreak @21:00:23.70 SE.configure :default @21:00:23.84 Elbow.reconfigure :limp @21:00:23.89 Forearm.reconfigure :loose @21:00:23.93 Forearm.seek 1 @21:00:24.33 Elbow.hold @21:00:24.39 Elbow.reconfigure :tight @21:00:24.48 SE.seek 7.26 Pucks below Top Plate @21:00:24.91 Forearm.force -20 @21:00:24.94 Forearm.seek 1 + 319 counts @21:00:25.26 Forearm.reconfigure :tight @21:00:25.30 Forearm.seek 1 + 700 counts @21:00:25.49 SE.seek 8.26 Pucks below Top Plate @21:00:26.11 Forearm.reconfigure :loose @21:00:26.15 Forearm.seek :retract @21:00:27.05 Elbow.seek CC @21:00:28.66 Elbow.reconfigure :loose @21:00:28.74 Forearm.seek CC @21:00:29.70 Hand.close @21:00:30.30 CC.seek :open @21:00:34.72 Forearm.seek :retract @21:00:35.72 Elbow.reconfigure :tight @21:00:35.77 Elbow.seek 1 @21:00:37.62 Elbow.reconfigure :limp @21:00:37.67 Forearm.seek 1 @21:00:38.78 Elbow.hold @21:00:38.84 Elbow.reconfigure :tight @21:00:38.88 Hand.open @21:00:39.79 Forearm.force -20 @21:00:39.83 Forearm.seek 1 + 318 counts @21:00:40.10 Forearm.reconfigure :tight @21:00:40.14 Forearm.seek 1 + 700 counts @21:00:40.47 SE.seek :down @21:00:41.59
Forearm.reconfigure :loose @21:00:41.64 Forearm.seek :retract @21:00:42.62 SE.stop @21:00:42.68
Tranferring DA @21:00:42.69 Beginning samplePassC @21:00:42.70 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:00:42.87 Elbow.seek FlushPuck::Garage @21:00:44.60 Elbow.reconfigure :loose @21:00:44.70 Forearm.seek :garage @21:00:45.78 Hand.close @21:00:46.53 Forearm.seek :retract @21:00:47.68 Elbow.reconfigure :tight @21:00:47.72 Elbow.seek CC @21:00:50.66 Elbow.reconfigure :loose @21:00:50.75 Forearm.reconfigure :tight @21:00:50.79 Forearm.seek CC @21:00:52.10 CC.seek :closed @21:00:56.30 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.88 @21:00:56.37 Hand.open @21:00:57.11 Forearm.reconfigure :loose @21:00:57.15 Forearm.seek :retract @21:00:58.20 PSV.dial PSR @21:00:59.99 Pulling 1.5ml of PSR.flush @21:01:00.00 PSR.select :flush @21:01:00.12 PS.seek 1.5ml @21:01:16.09 delay 5 seconds @21:01:21.11 PSV.dial :waste1 @21:01:21.55 PS.seek 0ml @21:01:37.65 PSV.dial PSR @21:01:38.20 PSR.select :air @21:01:38.31 PS.seek 2.5ml @21:02:04.56 PRV.dial CBV,avoiding: :ab2 @21:02:04.59
CBV.dial PRV @21:02:06.21 PSV.dial :mixloop @21:02:07.22 CTV.dial :air,avoiding: :intake @21:02:07.23 PTV.dial :PRVmixing @21:02:08.44 PS.seek 0ml @21:02:34.56 PSV.dial :air @21:02:34.98 PS.seek 2.5ml @21:03:01.15 PSV.dial :mixloop @21:03:01.59 PS.seek 0ml @21:03:27.80 PSV.dial :air @21:03:28.25 PS.seek 2.5ml @21:03:54.40 PSV.dial :mixloop @21:03:54.95 PS.seek 0ml @21:04:21.21 Garaging FlushPuck in Collection Clamp @21:04:21.28 Elbow.reconfigure :tight @21:04:21.32 Elbow.seek CC @21:04:21.69 Elbow.reconfigure :loose @21:04:21.78 Forearm.seek CC @21:04:22.77 Hand.close @21:04:23.54 CC.seek :open @21:04:27.86 Forearm.seek :retract @21:04:28.84 Elbow.reconfigure :tight @21:04:28.88 Elbow.seek :garage @21:04:31.91 Elbow.reconfigure :loose @21:04:32.00 Forearm.reconfigure :tight @21:04:32.04 Forearm.seek :garage @21:04:33.17 Hand.open @21:04:33.97 Forearm.reconfigure :loose @21:04:34.02 Forearm.seek :retract @21:04:35.09 PSV.dial :mixloop @21:04:35.10 PTV.dial :PRVmixing @21:04:35.11 PRV.dial CBV @21:04:35.14
CBV.dial PRV @21:04:35.15 CTV.dial :air,avoiding: :intake @21:04:35.17 Loading lyfil puck from tube 2 into Collection Clamp @21:04:35.38 SC.reconfigure :tight @21:04:35.42 SC.seek 2 @21:04:39.00 SC.configure :limp @21:04:39.20 Elbow.reconfigure :tight @21:04:39.24 Elbow.seek 2 @21:04:41.17 Forearm.reconfigure :tight @21:04:41.21 Forearm.seek 2 + 700 counts @21:04:42.27 SE.reconfigure :pick @21:04:42.32 SE.seek :beamBreak @21:04:43.83 SE.configure :default @21:04:43.98 Elbow.seek 2 @21:04:44.16 Forearm.seek 2 + 300 counts @21:04:44.32 Forearm.reconfigure :loose @21:04:44.36 Hand.close @21:04:44.93 SE.seek :down @21:04:45.11
Forearm.seek :retract @21:04:46.17 Elbow.seek CC @21:04:46.19 SE.stop @21:04:47.71
Elbow.reconfigure :loose @21:04:47.79 Forearm.reconfigure :tight @21:04:47.84 Forearm.seek CC @21:04:49.08 CC.seek :closed @21:04:53.40 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.69 @21:04:53.41 Hand.open @21:04:54.23 Forearm.reconfigure :loose @21:04:54.27 Forearm.seek :retract @21:04:55.34 CTV.dial CSV,avoiding: :intake @21:04:56.60 CSV.dial CTV @21:04:57.03 PS.seek 2.5ml @21:05:23.30 CS.seek 5.5ml @21:05:35.51 delay 8 seconds @21:05:43.53 PSV.dial :air @21:05:44.07 delay 2 seconds @21:05:46.08 PSV.dial :waste2 @21:05:46.82 PS.seek 0ml @21:06:13.08 CBV.dial between PRV and waste1 @21:06:13.39 CSV.dial :air @21:06:13.82 CS.seek 10ml @21:06:25.97 CSV.dial CTV @21:06:26.40 CS.seek 0ml @21:06:52.40 CBV.dial :waste1 @21:06:52.70 delay 5 seconds @21:06:57.72 CTV.dial :air,avoiding: :intake @21:06:59.02 Storing lyfil puck in Collection Clamp to tube 1 @21:06:59.18 SC.reconfigure :tight @21:06:59.22 SC.seek 1 @21:07:02.89 SC.configure :limp @21:07:03.09 Elbow.reconfigure :tight @21:07:03.13 Elbow.seek 1 @21:07:04.99 Forearm.reconfigure :tight @21:07:05.03 Forearm.seek 1 + 700 counts @21:07:05.97 SE.reconfigure :pick @21:07:06.02 SE.seek :beamBreak @21:07:08.55 SE.configure :default @21:07:08.69 Elbow.reconfigure :limp @21:07:08.74 Forearm.reconfigure :loose @21:07:08.78 Forearm.seek 1 @21:07:09.19 Elbow.hold @21:07:09.25 Elbow.reconfigure :tight @21:07:09.34 SE.seek 8.27 Pucks below Top Plate @21:07:09.85 Forearm.force -20 @21:07:09.89 Forearm.seek 1 + 312 counts @21:07:10.28 Forearm.reconfigure :tight @21:07:10.32 Forearm.seek 1 + 700 counts @21:07:10.53 SE.seek 9.27 Pucks below Top Plate @21:07:11.10 Forearm.reconfigure :loose @21:07:11.14 Forearm.seek :retract @21:07:12.13 Elbow.seek CC @21:07:13.74 Elbow.reconfigure :loose @21:07:13.82 Forearm.seek CC @21:07:14.80 Hand.close @21:07:15.41 CC.seek :open @21:07:19.87 Forearm.seek :retract @21:07:20.94 Elbow.reconfigure :tight @21:07:20.98 Elbow.seek 1 @21:07:22.66 Elbow.reconfigure :limp @21:07:22.70 Forearm.seek 1 @21:07:23.80 Elbow.hold @21:07:23.86 Elbow.reconfigure :tight @21:07:23.90 Hand.open @21:07:24.78 Forearm.force -20 @21:07:24.82 Forearm.seek 1 + 316 counts @21:07:25.09 Forearm.reconfigure :tight @21:07:25.13 Forearm.seek 1 + 700 counts @21:07:25.50 SE.seek :down @21:07:26.63
Forearm.reconfigure :loose @21:07:26.67 Forearm.seek :retract @21:07:27.59 SE.stop @21:07:28.12
PSV.dial :air @21:07:28.84 PS.seek 2.5ml @21:07:55.05 delay 1 second @21:07:56.06 PSV.dial :mixloop @21:07:56.49 delay 1 second @21:07:57.50 PSV.dial :air @21:07:57.94 delay 1 second @21:07:58.95 PSV.dial :mixloop @21:07:59.38 delay 1 second @21:08:00.39 PSV.dial :air @21:08:00.83 delay 1 second @21:08:01.85 PS.seek 0ml @21:08:28.18 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @21:08:28.35 Elbow.seek FlushPuck::Garage @21:08:30.10 Elbow.reconfigure :loose @21:08:30.19 Forearm.seek :garage @21:08:31.27 Hand.close @21:08:32.02 Forearm.seek :retract @21:08:33.21 Elbow.reconfigure :tight @21:08:33.26 Elbow.seek PC @21:08:34.65 Elbow.reconfigure :loose @21:08:34.74 Forearm.reconfigure :tight @21:08:34.78 Forearm.seek PC @21:08:36.00 PC.seek :closed @21:08:39.90 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.44 @21:08:39.97 Hand.open @21:08:40.72 Forearm.reconfigure :loose @21:08:40.76 Forearm.seek :retract @21:08:41.75 PRV.dial :air @21:08:42.19 PSV.dial :mixloop @21:08:42.68 PS.seek 0.3ml @21:08:46.54 PSV.dial PSR @21:08:47.57 Pulling 0.4ml of PSR.air @21:08:47.58 PSR.select :air @21:08:47.60 PS.seek 0.7ml @21:08:52.55 delay 3 seconds @21:08:55.56 PSR.select :air @21:08:55.58 PS.seek 2.5ml @21:09:14.64 delay 3 seconds @21:09:17.65 PSV.dial :mixloop @21:09:18.62 PTV.dial :mixing @21:09:19.33 PBV.dial :waste2 @21:09:20.19 PS.seek 0ml @21:09:46.39 Garaging FlushPuck in Processing Clamp @21:09:46.46 Elbow.reconfigure :tight @21:09:46.50 Elbow.seek PC @21:09:46.68 Elbow.reconfigure :loose @21:09:46.77 Forearm.seek PC @21:09:47.87 Hand.close @21:09:48.53 PC.seek :open @21:09:52.50 Forearm.seek :retract @21:09:53.52 Elbow.reconfigure :tight @21:09:53.56 Elbow.seek :garage @21:09:54.94 Elbow.reconfigure :loose @21:09:55.03 Forearm.reconfigure :tight @21:09:55.07 Forearm.seek :garage @21:09:56.23 Hand.open @21:09:56.96 Forearm.reconfigure :loose @21:09:57.00 Forearm.seek :retract @21:09:58.03 Loading da puck from tube 2 into Processing Clamp @21:09:58.25 SC.reconfigure :tight @21:09:58.29 SC.seek 2 @21:10:01.87 SC.configure :limp @21:10:02.06 Elbow.reconfigure :tight @21:10:02.10 Elbow.seek 2 @21:10:04.05 Forearm.reconfigure :tight @21:10:04.09 Forearm.seek 2 + 700 counts @21:10:05.15 SE.reconfigure :pick @21:10:05.20 SE.seek :beamBreak @21:10:06.89 SE.configure :default @21:10:07.03 Elbow.seek 2 @21:10:07.83 Forearm.seek 2 + 300 counts @21:10:08.19 Forearm.reconfigure :loose @21:10:08.23 Hand.close @21:10:08.79 SE.seek :down @21:10:08.99
Forearm.seek :retract @21:10:10.02 Elbow.seek PC @21:10:10.09 SE.stop @21:10:12.58
Elbow.reconfigure :loose @21:10:12.67 Forearm.reconfigure :tight @21:10:12.72 Forearm.seek PC @21:10:14.07 PC.seek :closed @21:10:17.95 Processing Clamp onSpring/offSpring for 8/23 samples, on/off torque 1.40 @21:10:17.96 Hand.open @21:10:18.71 Forearm.reconfigure :loose @21:10:18.75 Forearm.seek :retract @21:10:19.79 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @21:10:19.80 PH.seek 28C,5:00 @21:11:13.24 PH.hold 2:00:00,28C @21:11:13.28 delay 1:30 @21:12:43.32 PSR.select :air @21:12:43.34 PSV.dial PSR @21:12:44.38 Pulling 0.5ml of PSR.wash PSR.select :wash @21:12:44.50 PS.seek 0.5ml @21:12:50.45 delay 5 seconds @21:12:55.47 PSR.select :air @21:12:55.58 PS.seek 2ml @21:13:11.79 PTV.dial :puck @21:13:12.48 PSV.dial PTV @21:13:13.18 PBV.dial :waste1 @21:13:14.46 PS.seek 1.5ml @21:13:20.38 delay 3 seconds @21:13:23.40 PBV.dial between PSV and waste1 @21:13:23.77 PS.seek 0ml @21:13:39.79 delay 5:00 @21:18:39.81 recoverPSRreagent08 PTV.dial :puck @21:18:39.82 PBV.dial :waste1 @21:18:40.17 delay 30 seconds @21:19:10.19 PBV.dial between PSV and waste1 @21:19:10.55 PSR.select :air @21:19:10.56 PSV.dial PSR @21:19:11.39 PS.reconfigure :fast @21:19:11.44 PS.seek 2.5ml @21:19:34.15 PSV.dial PTV @21:19:34.83 delay 5 seconds @21:19:39.84 PSV.dial PSR @21:19:40.54 delay 5 seconds @21:19:45.56 PSV.dial PTV @21:19:46.26 delay 5 seconds @21:19:51.28 PSV.dial :air @21:19:51.99 PS.seek 0ml @21:20:14.62 PTV.dial :PRVmixing,via: PRV @21:20:15.85 PSV.dial :mixloop @21:20:16.40 PRV.dial :ab1 @21:20:17.13 PS.reconfigure :slow @21:20:17.17 PS.seek 0.15ml @21:20:20.68 delay 2 seconds @21:20:22.70 CBV.dial PRV @21:20:22.75
PRV.dial CBV @21:20:23.26 PS.seek 0.34ml @21:20:27.62 delay 2 seconds @21:20:29.64 CBV.dial between PRV and PSV @21:20:30.43 PRV.dial :air @21:20:30.88 PS.seek 0.9ml @21:20:42.77 PRV.dial :waste2 @21:20:43.49 PS.seek 0.5ml @21:20:52.15 addPMLReagent08 @21:20:52.16 PSV.dial :air @21:20:52.58 PS.reconfigure :default @21:20:52.62 PS.seek 2ml @21:21:08.62 PSV.dial :PML @21:21:09.04 PTV.dial :mixing @21:21:09.76 PBV.dial :waste1 @21:21:10.18 PS.seek 1.85ml @21:21:12.56 PBV.dial between PSV and waste1 @21:21:12.94 PS.seek 0ml @21:21:32.46 delay 2 seconds @21:21:34.48 Beginning samplePassC @21:21:34.49 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:21:34.66 Elbow.reconfigure :tight @21:21:34.70 Elbow.seek FlushPuck::Garage @21:21:36.17 Elbow.reconfigure :loose @21:21:36.26 Forearm.seek :garage @21:21:37.37 Hand.close @21:21:38.12 Forearm.seek :retract @21:21:39.24 Elbow.reconfigure :tight @21:21:39.28 Elbow.seek CC @21:21:42.05 Elbow.reconfigure :loose @21:21:42.14 Forearm.reconfigure :tight @21:21:42.18 Forearm.seek CC @21:21:43.44 CC.seek :closed @21:21:47.66 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.87 @21:21:47.73 Hand.open @21:21:48.57 Forearm.reconfigure :loose @21:21:48.61 Forearm.seek :retract @21:21:49.61 PSV.dial PSR @21:21:50.64 Pulling 1.5ml of PSR.flush @21:21:50.65 PSR.select :flush @21:21:50.77 PS.seek 1.5ml @21:22:06.84 delay 5 seconds @21:22:11.86 PSV.dial :waste1 @21:22:12.29 PS.seek 0ml @21:22:28.52 PSV.dial PSR @21:22:28.94 PSR.select :air @21:22:29.05 PS.seek 2.5ml @21:22:55.21 PRV.dial CBV,avoiding: :ab2 @21:22:55.24
CBV.dial PRV @21:22:56.79 PSV.dial :mixloop @21:22:57.77 CTV.dial :air,avoiding: :intake @21:22:57.79 PTV.dial :PRVmixing @21:22:58.50 PS.seek 0ml @21:23:24.68 PSV.dial :air @21:23:25.10 PS.seek 2.5ml @21:23:51.33 PSV.dial :mixloop @21:23:51.78 PS.seek 0ml @21:24:17.99 PSV.dial :air @21:24:18.43 PS.seek 2.5ml @21:24:44.55 PSV.dial :mixloop @21:24:45.10 PS.seek 0ml @21:25:11.27 Garaging FlushPuck in Collection Clamp @21:25:11.34 Elbow.reconfigure :tight @21:25:11.38 Elbow.seek CC @21:25:11.49 Elbow.reconfigure :loose @21:25:11.58 Forearm.seek CC @21:25:12.53 Hand.close @21:25:13.25 CC.seek :open @21:25:17.58 Forearm.seek :retract @21:25:18.69 Elbow.reconfigure :tight @21:25:18.73 Elbow.seek :garage @21:25:21.79 Elbow.reconfigure :loose @21:25:22.32 Forearm.reconfigure :tight @21:25:22.39 Forearm.seek :garage @21:25:23.51 Hand.open @21:25:24.31 Forearm.reconfigure :loose @21:25:24.35 Forearm.seek :retract @21:25:25.36 delayUntil "21:29:34.47" @21:25:25.39 Whole Cell Archive sampling 1000ml @21:25:25.40 Loading wcr puck from tube 2 into Collection Clamp @21:25:25.63 SC.reconfigure :tight @21:25:25.68 SC.seek 2 @21:25:25.80 SC.configure :limp @21:25:26.00 Elbow.reconfigure :tight @21:25:26.05 Elbow.seek 2 @21:25:27.97 Forearm.reconfigure :tight @21:25:28.02 Forearm.seek 2 + 700 counts @21:25:29.09 SE.reconfigure :pick @21:25:29.15 SE.seek :beamBreak @21:25:30.87 SE.configure :default @21:25:31.01 Elbow.seek 2 @21:25:31.19 Forearm.seek 2 + 300 counts @21:25:31.45 Forearm.reconfigure :loose @21:25:31.49 Hand.close @21:25:32.14 SE.seek :down @21:25:32.33 Forearm.seek :retract @21:25:33.36 Elbow.seek CC @21:25:33.53 SE.stop @21:25:34.93 Elbow.reconfigure :loose @21:25:35.02 Forearm.reconfigure :tight @21:25:35.07 Forearm.seek CC @21:25:36.40 CC.seek :closed @21:25:40.65 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.66 @21:25:40.66 Hand.open @21:25:41.43 Forearm.reconfigure :loose @21:25:41.48 Forearm.seek :retract @21:25:42.53 collectSample 1000ml w/bubble point of 35psi @21:25:42.54 Priming 7ml @21:25:42.55 Intake.select :close @21:25:42.56 Exhaust.select :close @21:25:42.58 SS.hold @21:25:42.77 Calibrating pressure sensors @21:25:42.78 CTV.dial :air @21:25:42.80 SSV.dial :spit,avoiding: :exhaust @21:25:42.82 SS.reconfigure :fast @21:25:42.86 SS.seek 5ml @21:25:45.65 SSV.dial CBV @21:25:45.68 CBV.dial SSV @21:25:46.99 delay 3.8 seconds @21:25:50.90 SSV.dial :spit @21:25:52.18 SS.seek 0ml @21:25:55.28 SSV.dial CBV @21:25:56.57 CTV.dial :intake @21:25:57.28 SSV.dial CBV @21:25:57.31 CBV.dial SSV @21:25:57.33 Allowing 40s for 7.0ml of remaining 7.0ml @21:25:57.34 Intake.select :open @21:25:59.49 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:25:59.51 SS.reconfigure :inhaling 2.14ml/s @21:25:59.55 SS.seek 7ml @21:26:05.37 Stroke completed -- waiting for pressure to equalize ... @21:26:05.39 SS.reconfigure :exhale @21:26:05.43 SS.hold @21:26:10.43 Intake.select :close @21:26:10.57 SSV.dial :exhaust @21:26:11.30 Exhaust.select :open @21:26:11.41 SS.seek 0ml @21:26:15.42 Exhaust.select :close @21:26:15.57 Sampling 1000.0ml @21:26:15.59 Intake.select :close @21:26:15.61 Exhaust.select :close @21:26:15.63 SS.reconfigure :default @21:26:15.67 SS.hold @21:26:15.86 CTV.dial :intake @21:26:15.87 SSV.dial CBV @21:26:15.90 CBV.dial SSV @21:26:16.64 Allowing 130s for 25.0ml of remaining 1000.0ml @21:26:16.66 Intake.select :open @21:26:18.81 Intake @15.3psia, vacuum from 9.8 to 12.8psi @21:26:18.83 SS.reconfigure :inhaling 2.14ml/s @21:26:18.87 SS.seek 25ml @21:26:24.77 Vacuum too high -- waiting up to 40 seconds @21:26:24.79 SS.reconfigure :exhale @21:26:24.83 SS.hold @21:26:26.09 Intake @12.5psia, vacuum from 7.0 to 10.0psi @21:26:26.11 SS.reconfigure :inhaling 1.93ml/s @21:26:26.16 SS.seek 25ml @21:26:28.74 Vacuum too high -- waiting up to 40 seconds @21:26:28.75 SS.reconfigure :exhale @21:26:28.80 SS.hold @21:26:30.43 Intake @13.0psia, vacuum from 7.5 to 10.5psi @21:26:30.45 SS.reconfigure :inhaling 1.74ml/s @21:26:30.49 SS.seek 25ml @21:26:33.96 Vacuum too high -- waiting up to 40 seconds @21:26:33.97 SS.reconfigure :exhale @21:26:34.01 SS.hold @21:26:35.84 Intake @13.6psia, vacuum from 8.1 to 11.1psi @21:26:35.86 SS.reconfigure :inhaling 1.57ml/s @21:26:35.90 SS.seek 25ml @21:26:39.59 Vacuum too high -- waiting up to 40 seconds @21:26:39.60 SS.reconfigure :exhale @21:26:39.64 SS.hold @21:26:41.09 Intake @13.3psia, vacuum from 7.8 to 10.8psi @21:26:41.11 SS.reconfigure :inhaling 1.41ml/s @21:26:41.15 SS.seek 25ml @21:26:45.19 Vacuum too high -- waiting up to 40 seconds @21:26:45.20 SS.reconfigure :exhale @21:26:45.24 SS.hold @21:26:46.96 Intake @13.3psia, vacuum from 7.8 to 10.8psi @21:26:46.98 SS.reconfigure :inhaling 1.27ml/s @21:26:47.03 SS.seek 25ml @21:26:50.77 Stroke completed -- waiting for pressure to equalize ... @21:26:50.78 SS.reconfigure :exhale @21:26:50.82 SS.hold @21:26:52.81 Intake.select :close @21:26:52.95 SSV.dial :exhaust @21:26:53.69 Exhaust.select :open @21:26:53.80 SS.seek 0ml @21:27:02.65 Exhaust.select :close @21:27:02.76 SSV.dial CBV @21:27:02.79 CBV.dial SSV @21:27:03.55 Allowing 130s for 25.0ml of remaining 975.0ml @21:27:03.56 Intake.select :open @21:27:05.71 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:27:05.73 SS.reconfigure :inhaling 1.27ml/s @21:27:05.78 SS.seek 25ml @21:27:10.74 Vacuum too high -- waiting up to 40 seconds @21:27:10.75 SS.reconfigure :exhale @21:27:10.80 SS.hold @21:27:11.23 Intake @12.6psia, vacuum from 7.1 to 10.1psi @21:27:11.25 SS.reconfigure :inhaling 1.14ml/s @21:27:11.29 SS.seek 25ml @21:27:16.15 Vacuum too high -- waiting up to 40 seconds @21:27:16.74 SS.reconfigure :exhale @21:27:16.79 SS.hold @21:27:17.46 Intake @13.5psia, vacuum from 8.0 to 11.0psi @21:27:17.48 SS.reconfigure :inhaling 1.03ml/s @21:27:17.52 SS.seek 25ml @21:27:27.30 Vacuum too high -- waiting up to 40 seconds @21:27:27.32 SS.reconfigure :exhale @21:27:27.36 SS.hold @21:27:28.65 Intake @13.4psia, vacuum from 7.9 to 10.9psi @21:27:28.67 SS.reconfigure :inhaling 0.93ml/s @21:27:28.71 SS.seek 25ml @21:27:37.29 Stroke completed -- waiting for pressure to equalize ... @21:27:37.31 SS.reconfigure :exhale @21:27:37.35 SS.hold @21:27:39.39 Intake.select :close @21:27:39.54 SSV.dial :exhaust @21:27:40.26 Exhaust.select :open @21:27:40.37 SS.seek 0ml @21:27:49.67 Exhaust.select :close @21:27:49.78 SSV.dial CBV @21:27:49.81 CBV.dial SSV @21:27:50.53 Allowing 130s for 25.0ml of remaining 950.0ml @21:27:50.54 Intake.select :open @21:27:52.70 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:27:52.71 SS.reconfigure :inhaling 0.93ml/s @21:27:52.76 SS.seek 25ml @21:28:18.14 Vacuum too high -- waiting up to 40 seconds @21:28:18.16 SS.reconfigure :exhale @21:28:18.20 SS.hold @21:28:19.15 Intake @13.4psia, vacuum from 7.9 to 10.9psi @21:28:19.17 SS.reconfigure :inhaling 0.84ml/s @21:28:19.21 SS.seek 25ml @21:28:23.28 Stroke completed -- waiting for pressure to equalize ... @21:28:23.30 SS.reconfigure :exhale @21:28:23.34 SS.hold @21:28:24.92 Intake.select :close @21:28:25.07 SSV.dial :exhaust @21:28:25.79 Exhaust.select :open @21:28:25.91 SS.seek 0ml @21:28:34.75 Exhaust.select :close @21:28:34.85 SSV.dial CBV @21:28:34.88 CBV.dial SSV @21:28:35.64 Allowing 130s for 25.0ml of remaining 925.0ml @21:28:35.65 Intake.select :open @21:28:37.81 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:28:37.82 SS.reconfigure :inhaling 0.84ml/s @21:28:37.87 SS.seek 25ml @21:29:10.10 Stroke completed -- waiting for pressure to equalize ... @21:29:10.12 SS.reconfigure :exhale @21:29:10.16 SS.hold @21:29:12.66 Intake.select :close @21:29:12.80 SSV.dial :exhaust @21:29:13.53 Exhaust.select :open @21:29:13.64 SS.seek 0ml @21:29:22.59 Exhaust.select :close @21:29:22.69 SSV.dial CBV @21:29:22.72 CBV.dial SSV @21:29:23.47 Allowing 130s for 25.0ml of remaining 900.0ml @21:29:23.48 Intake.select :open @21:29:25.63 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:29:25.65 SS.reconfigure :inhaling 0.84ml/s @21:29:25.69 SS.seek 25ml @21:29:34.48 recoverPRVreagent08 @21:29:34.49 PTV.dial :mixing @21:29:35.20 PBV.dial :waste1 @21:29:35.56 delay 30 seconds @21:29:58.10 Stroke completed -- waiting for pressure to equalize ... @21:29:58.11 SS.reconfigure :exhale @21:29:58.16 SS.hold @21:30:00.66 Intake.select :close @21:30:01.26 SSV.dial :exhaust @21:30:02.01 Exhaust.select :open @21:30:02.12 SS.seek 0ml @21:30:05.57 PBV.dial between PSV and waste1 @21:30:05.93 PSV.dial :air @21:30:06.37 PS.reconfigure :fast @21:30:06.42 PS.seek 2.5ml @21:30:11.45 Exhaust.select :close @21:30:11.56 SSV.dial CBV @21:30:11.58 CBV.dial SSV @21:30:12.34 Allowing 130s for 25.0ml of remaining 875.0ml @21:30:12.35 Intake.select :open @21:30:14.51 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:30:14.52 SS.reconfigure :inhaling 0.84ml/s @21:30:14.57 SS.seek 25ml @21:30:29.23 PSV.dial :mixloop @21:30:29.68 delay 2 seconds @21:30:31.70 PSV.dial :air @21:30:32.15 delay 2 seconds @21:30:34.17 PSV.dial :mixloop @21:30:34.63 delay 2 seconds @21:30:36.64 PSV.dial :air @21:30:37.09 PS.seek 0ml @21:30:46.05 Stroke completed -- waiting for pressure to equalize ... @21:30:46.07 SS.reconfigure :exhale @21:30:46.11 SS.hold @21:30:49.02 Intake.select :close @21:30:49.16 SSV.dial :exhaust @21:30:49.88 Exhaust.select :open @21:30:49.99 SS.seek 0ml @21:30:58.91 Exhaust.select :close @21:30:59.02 SSV.dial CBV @21:30:59.06 CBV.dial SSV @21:30:59.78 Allowing 130s for 25.0ml of remaining 850.0ml @21:30:59.79 Intake.select :open @21:30:59.85 washCycleDA #1 of 2 @21:30:59.86 PSR.select :air @21:30:59.90 PSV.dial PSR @21:31:01.31 Pulling 0.5ml of PSR.wash @21:31:01.32 PSR.select :wash @21:31:01.43 PS.reconfigure :default @21:31:01.48 PS.seek 0.5ml @21:31:01.99 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:31:02.01 SS.reconfigure :inhaling 0.84ml/s @21:31:02.05 SS.seek 25ml @21:31:07.32 delay 5 seconds @21:31:12.34 PSR.select :air @21:31:12.45 PS.seek 2ml @21:31:26.11 Vacuum too high -- waiting up to 40 seconds @21:31:26.12 SS.reconfigure :exhale @21:31:26.16 SS.hold @21:31:27.12 Intake @13.3psia, vacuum from 7.8 to 10.8psi @21:31:27.19 SS.reconfigure :inhaling 0.76ml/s @21:31:27.23 SS.seek 25ml @21:31:28.44 PTV.dial :puck @21:31:29.15 PSV.dial PTV @21:31:29.99 PBV.dial :waste1 @21:31:30.41 PS.seek 1.5ml @21:31:36.41 delay 3 seconds @21:31:37.27 Stroke completed -- waiting for pressure to equalize ... @21:31:37.28 SS.reconfigure :exhale @21:31:37.32 SS.hold @21:31:39.37 Intake.select :close @21:31:39.43 PBV.dial between PSV and waste1 @21:31:39.52 SSV.dial :exhaust @21:31:39.80 PS.seek 0ml @21:31:40.24 Exhaust.select :open @21:31:40.35 SS.seek 0ml @21:31:49.72 Exhaust.select :close @21:31:49.83 SSV.dial CBV @21:31:49.87 CBV.dial SSV @21:31:50.60 Allowing 130s for 25.0ml of remaining 825.0ml @21:31:50.61 Intake.select :open @21:31:52.77 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:31:52.78 SS.reconfigure :inhaling 0.76ml/s @21:31:52.83 SS.seek 25ml @21:31:55.88 delay 2:00 @21:32:27.69 Stroke completed -- waiting for pressure to equalize ... @21:32:27.70 SS.reconfigure :exhale @21:32:27.74 SS.hold @21:32:30.42 Intake.select :close @21:32:30.57 SSV.dial :exhaust @21:32:31.30 Exhaust.select :open @21:32:31.41 SS.seek 0ml @21:32:40.41 Exhaust.select :close @21:32:40.52 SSV.dial CBV @21:32:40.55 CBV.dial SSV @21:32:41.27 Allowing 130s for 25.0ml of remaining 800.0ml @21:32:41.29 Intake.select :open @21:32:43.44 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:32:43.46 SS.reconfigure :inhaling 0.76ml/s @21:32:43.50 SS.seek 25ml @21:33:18.70 Stroke completed -- waiting for pressure to equalize ... @21:33:18.72 SS.reconfigure :exhale @21:33:18.76 SS.hold @21:33:21.66 Intake.select :close @21:33:21.81 SSV.dial :exhaust @21:33:22.53 Exhaust.select :open @21:33:22.65 SS.seek 0ml @21:33:31.84 Exhaust.select :close @21:33:31.95 SSV.dial CBV @21:33:31.98 CBV.dial SSV @21:33:32.74 Allowing 130s for 25.0ml of remaining 775.0ml @21:33:32.80 Intake.select :open @21:33:34.95 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:33:34.97 SS.reconfigure :inhaling 0.76ml/s @21:33:35.01 SS.seek 25ml @21:33:55.91 recoverPSRreagent08 @21:33:55.92 PTV.dial :puck @21:33:55.93 PBV.dial :waste1 @21:33:56.29 delay 30 seconds @21:34:09.95 Stroke completed -- waiting for pressure to equalize ... @21:34:09.96 SS.reconfigure :exhale @21:34:10.01 SS.hold @21:34:12.99 Intake.select :close @21:34:13.14 SSV.dial :exhaust @21:34:13.86 Exhaust.select :open @21:34:13.97 SS.seek 0ml @21:34:23.37 Exhaust.select :close @21:34:23.48 SSV.dial CBV @21:34:23.51 CBV.dial SSV @21:34:24.25 Allowing 130s for 25.0ml of remaining 750.0ml @21:34:24.26 Intake.select :open @21:34:26.30 PBV.dial between PSV and waste1 @21:34:26.42 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:34:26.43 SS.reconfigure :inhaling 0.76ml/s @21:34:26.48 SS.seek 25ml @21:34:26.66 PSR.select :air @21:34:26.68 PSV.dial PSR @21:34:27.38 PS.reconfigure :fast @21:34:27.42 PS.seek 2.5ml @21:34:50.28 PSV.dial PTV @21:34:51.00 delay 5 seconds @21:34:56.02 PSV.dial PSR @21:34:56.74 delay 5 seconds @21:35:01.52 Stroke completed -- waiting for pressure to equalize ... @21:35:01.53 SS.reconfigure :exhale @21:35:01.57 SS.hold @21:35:01.80 PSV.dial PTV @21:35:02.63 delay 5 seconds @21:35:05.07 Intake.select :close @21:35:05.21 SSV.dial :exhaust @21:35:05.94 Exhaust.select :open @21:35:06.05 SS.seek 0ml @21:35:07.64 PSV.dial :air @21:35:08.38 PS.seek 0ml @21:35:15.01 Exhaust.select :close @21:35:15.12 SSV.dial CBV @21:35:15.15 CBV.dial SSV @21:35:15.91 Allowing 130s for 25.0ml of remaining 725.0ml @21:35:15.92 Intake.select :open @21:35:18.07 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:35:18.09 SS.reconfigure :inhaling 0.76ml/s @21:35:18.13 SS.seek 25ml @21:35:31.74 washCycleDA #2 of 2 @21:35:31.77 PSR.select :air @21:35:31.79 PSV.dial PSR @21:35:33.08 Pulling 0.5ml of PSR.wash @21:35:33.09 PSR.select :wash @21:35:33.21 PS.reconfigure :default @21:35:33.25 PS.seek 0.5ml @21:35:39.10 delay 5 seconds @21:35:44.12 PSR.select :air @21:35:44.23 PS.seek 2ml @21:35:53.98 Stroke completed -- waiting for pressure to equalize ... @21:35:53.99 SS.reconfigure :exhale @21:35:54.04 SS.hold @21:35:57.48 Intake.select :close @21:35:57.62 SSV.dial :exhaust @21:35:58.36 Exhaust.select :open @21:35:58.47 SS.seek 0ml @21:36:00.28 PTV.dial :puck @21:36:00.29 PSV.dial PTV @21:36:00.98 PBV.dial :waste1 @21:36:01.40 PS.seek 1.5ml @21:36:07.28 delay 3 seconds @21:36:07.76 Exhaust.select :close @21:36:07.87 SSV.dial CBV @21:36:07.91 CBV.dial SSV @21:36:08.62 Allowing 130s for 25.0ml of remaining 700.0ml @21:36:08.64 Intake.select :open @21:36:10.30 PBV.dial between PSV and waste1 @21:36:10.66 PS.seek 0ml @21:36:10.79 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:36:10.81 SS.reconfigure :inhaling 0.76ml/s @21:36:10.85 SS.seek 25ml @21:36:26.75 delay 2:00 @21:36:42.35 Vacuum too high -- waiting up to 40 seconds @21:36:42.36 SS.reconfigure :exhale @21:36:42.40 SS.hold @21:36:44.05 Intake @13.0psia, vacuum from 7.5 to 10.5psi @21:36:44.07 SS.reconfigure :inhaling 0.69ml/s @21:36:44.11 SS.seek 25ml @21:36:48.17 Stroke completed -- waiting for pressure to equalize ... @21:36:48.18 SS.reconfigure :exhale @21:36:48.22 SS.hold @21:36:50.17 Intake.select :close @21:36:50.31 SSV.dial :exhaust @21:36:51.04 Exhaust.select :open @21:36:51.15 SS.seek 0ml @21:37:00.57 Exhaust.select :close @21:37:00.68 SSV.dial CBV @21:37:00.73 CBV.dial SSV @21:37:01.44 Allowing 130s for 25.0ml of remaining 675.0ml @21:37:01.45 Intake.select :open @21:37:03.60 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:37:03.62 SS.reconfigure :inhaling 0.69ml/s @21:37:03.66 SS.seek 25ml @21:37:42.15 Stroke completed -- waiting for pressure to equalize ... @21:37:42.16 SS.reconfigure :exhale @21:37:42.20 SS.hold @21:37:45.25 Intake.select :close @21:37:45.39 SSV.dial :exhaust @21:37:46.12 Exhaust.select :open @21:37:46.23 SS.seek 0ml @21:37:55.18 Exhaust.select :close @21:37:55.29 SSV.dial CBV @21:37:55.31 CBV.dial SSV @21:37:56.04 Allowing 130s for 25.0ml of remaining 650.0ml @21:37:56.05 Intake.select :open @21:37:58.21 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:37:58.22 SS.reconfigure :inhaling 0.69ml/s @21:37:58.27 SS.seek 25ml @21:38:26.77 recoverPSRreagent08 @21:38:26.78 PTV.dial :puck @21:38:26.79 PBV.dial :waste1 @21:38:27.14 delay 30 seconds @21:38:37.68 Stroke completed -- waiting for pressure to equalize ... @21:38:37.69 SS.reconfigure :exhale @21:38:37.73 SS.hold @21:38:41.06 Intake.select :close @21:38:41.20 SSV.dial :exhaust @21:38:41.93 Exhaust.select :open @21:38:42.04 SS.seek 0ml @21:38:50.90 Exhaust.select :close @21:38:51.00 SSV.dial CBV @21:38:51.03 CBV.dial SSV @21:38:51.79 Allowing 130s for 25.0ml of remaining 625.0ml @21:38:51.80 Intake.select :open @21:38:53.96 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:38:53.98 SS.reconfigure :inhaling 0.69ml/s @21:38:54.02 SS.seek 25ml @21:38:57.15 PBV.dial between PSV and waste1 @21:38:57.51 PSR.select :air @21:38:57.53 PSV.dial PSR @21:38:58.25 PS.reconfigure :fast @21:38:58.29 PS.seek 2.5ml @21:39:20.99 PSV.dial PTV @21:39:21.71 delay 5 seconds @21:39:26.73 PSV.dial PSR @21:39:27.45 delay 5 seconds @21:39:32.47 PSV.dial PTV @21:39:33.18 delay 5 seconds @21:39:33.25 Stroke completed -- waiting for pressure to equalize ... @21:39:33.27 SS.reconfigure :exhale @21:39:33.31 SS.hold @21:39:37.83 Intake.select :close @21:39:37.98 SSV.dial :exhaust @21:39:38.20 PSV.dial :air @21:39:38.71 Exhaust.select :open @21:39:38.82 SS.seek 0ml @21:39:38.91 PS.seek 0ml @21:39:48.24 Exhaust.select :close @21:39:48.35 SSV.dial CBV @21:39:48.38 CBV.dial SSV @21:39:49.12 Allowing 130s for 25.0ml of remaining 600.0ml @21:39:49.13 Intake.select :open @21:39:51.28 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:39:51.30 SS.reconfigure :inhaling 0.69ml/s @21:39:51.34 SS.seek 25ml @21:40:01.49 getPMLReagent08 @21:40:01.50 PRV.dial :air @21:40:01.93 delay 5 seconds @21:40:06.95 PTV.dial :PRVmixing @21:40:08.20 delay 5 seconds @21:40:13.21 PRV.dial :ab2 @21:40:13.66 PTV.dial :PRVmixing @21:40:13.68 PSV.dial :mixloop @21:40:14.11 PS.reconfigure :default @21:40:14.15 PS.seek 0.3ml @21:40:17.96 delay 2 seconds @21:40:19.98 PRV.dial :air @21:40:20.49 PS.seek 0.55ml @21:40:23.80 addPMLReagent08 @21:40:23.81 PSV.dial :air @21:40:24.25 PS.seek 2ml @21:40:29.64 Stroke completed -- waiting for pressure to equalize ... @21:40:29.66 SS.reconfigure :exhale @21:40:29.70 SS.hold @21:40:33.76 Intake.select :close @21:40:33.90 SSV.dial :exhaust @21:40:34.63 Exhaust.select :open @21:40:34.75 SS.seek 0ml @21:40:39.75 PSV.dial :PML @21:40:40.30 PTV.dial :mixing @21:40:41.02 PBV.dial :waste1 @21:40:41.44 PS.seek 1.85ml @21:40:43.74 PBV.dial between PSV and waste1 @21:40:44.02 Exhaust.select :close @21:40:44.11 PS.seek 0ml @21:40:44.16 SSV.dial CBV @21:40:44.19 CBV.dial SSV @21:40:44.95 Allowing 130s for 25.0ml of remaining 575.0ml @21:40:45.45 Intake.select :open @21:40:47.60 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:40:47.62 SS.reconfigure :inhaling 0.69ml/s @21:40:47.66 SS.seek 25ml @21:41:03.71 delay 2 seconds @21:41:05.73 delay 8:00 @21:41:26.02 Stroke completed -- waiting for pressure to equalize ... @21:41:26.03 SS.reconfigure :exhale @21:41:26.07 SS.hold @21:41:30.84 Intake.select :close @21:41:30.98 SSV.dial :exhaust @21:41:31.72 Exhaust.select :open @21:41:31.83 SS.seek 0ml @21:41:41.16 Exhaust.select :close @21:41:41.26 SSV.dial CBV @21:41:41.29 CBV.dial SSV @21:41:42.04 Allowing 130s for 25.0ml of remaining 550.0ml @21:41:42.05 Intake.select :open @21:41:44.20 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:41:44.22 SS.reconfigure :inhaling 0.69ml/s @21:41:44.27 SS.seek 25ml @21:42:22.64 Stroke completed -- waiting for pressure to equalize ... @21:42:22.65 SS.reconfigure :exhale @21:42:22.69 SS.hold @21:42:27.48 Intake.select :close @21:42:27.62 SSV.dial :exhaust @21:42:28.36 Exhaust.select :open @21:42:28.47 SS.seek 0ml @21:42:37.37 Exhaust.select :close @21:42:37.48 SSV.dial CBV @21:42:37.51 CBV.dial SSV @21:42:38.27 Allowing 130s for 25.0ml of remaining 525.0ml @21:42:38.28 Intake.select :open @21:42:40.43 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:42:40.45 SS.reconfigure :inhaling 0.69ml/s @21:42:40.50 SS.seek 25ml @21:43:09.80 Vacuum too high -- waiting up to 40 seconds @21:43:09.82 SS.reconfigure :exhale @21:43:09.86 SS.hold @21:43:11.90 Intake @13.9psia, vacuum from 8.4 to 11.4psi @21:43:11.92 SS.reconfigure :inhaling 0.61ml/s @21:43:11.96 SS.seek 25ml @21:43:23.40 Stroke completed -- waiting for pressure to equalize ... @21:43:23.41 SS.reconfigure :exhale @21:43:23.46 SS.hold @21:43:27.00 Intake.select :close @21:43:27.14 SSV.dial :exhaust @21:43:27.87 Exhaust.select :open @21:43:27.98 SS.seek 0ml @21:43:37.34 Exhaust.select :close @21:43:37.45 SSV.dial CBV @21:43:37.48 CBV.dial SSV @21:43:38.20 Allowing 130s for 25.0ml of remaining 500.0ml @21:43:38.22 Intake.select :open @21:43:40.37 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:43:40.39 SS.reconfigure :inhaling 0.61ml/s @21:43:40.43 SS.seek 25ml @21:44:23.06 Stroke completed -- waiting for pressure to equalize ... @21:44:23.07 SS.reconfigure :exhale @21:44:23.11 SS.hold @21:44:27.15 Intake.select :close @21:44:27.30 SSV.dial :exhaust @21:44:28.03 Exhaust.select :open @21:44:28.15 SS.seek 0ml @21:44:37.28 Exhaust.select :close @21:44:37.38 SSV.dial CBV @21:44:37.41 CBV.dial SSV @21:44:38.17 Allowing 130s for 25.0ml of remaining 475.0ml @21:44:38.18 Intake.select :open @21:44:40.34 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:44:40.36 SS.reconfigure :inhaling 0.61ml/s @21:44:40.40 SS.seek 25ml @21:45:23.15 Stroke completed -- waiting for pressure to equalize ... @21:45:23.16 SS.reconfigure :exhale @21:45:23.20 SS.hold @21:45:27.98 Intake.select :close @21:45:28.12 SSV.dial :exhaust @21:45:28.85 Exhaust.select :open @21:45:28.97 SS.seek 0ml @21:45:38.29 Exhaust.select :close @21:45:38.40 SSV.dial CBV @21:45:38.42 CBV.dial SSV @21:45:39.15 Allowing 130s for 25.0ml of remaining 450.0ml @21:45:39.17 Intake.select :open @21:45:41.32 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:45:41.34 SS.reconfigure :inhaling 0.61ml/s @21:45:41.38 SS.seek 25ml @21:46:24.05 Stroke completed -- waiting for pressure to equalize ... @21:46:24.07 SS.reconfigure :exhale @21:46:24.11 SS.hold @21:46:28.96 Intake.select :close @21:46:29.11 SSV.dial :exhaust @21:46:29.84 Exhaust.select :open @21:46:29.95 SS.seek 0ml @21:46:39.24 Exhaust.select :close @21:46:39.35 SSV.dial CBV @21:46:39.38 CBV.dial SSV @21:46:40.10 Allowing 130s for 25.0ml of remaining 425.0ml @21:46:40.12 Intake.select :open @21:46:42.27 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:46:42.29 SS.reconfigure :inhaling 0.61ml/s @21:46:42.33 SS.seek 25ml @21:47:25.02 Stroke completed -- waiting for pressure to equalize ... @21:47:25.03 SS.reconfigure :exhale @21:47:25.07 SS.hold @21:47:29.93 Intake.select :close @21:47:30.08 SSV.dial :exhaust @21:47:30.79 Exhaust.select :open @21:47:30.90 SS.seek 0ml @21:47:39.85 Exhaust.select :close @21:47:39.95 SSV.dial CBV @21:47:39.98 CBV.dial SSV @21:47:40.71 Allowing 130s for 25.0ml of remaining 400.0ml @21:47:40.72 Intake.select :open @21:47:42.88 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:47:42.89 SS.reconfigure :inhaling 0.61ml/s @21:47:42.94 SS.seek 25ml @21:48:26.55 Stroke completed -- waiting for pressure to equalize ... @21:48:26.56 SS.reconfigure :exhale @21:48:26.61 SS.hold @21:48:32.27 Intake.select :close @21:48:32.41 SSV.dial :exhaust @21:48:33.14 Exhaust.select :open @21:48:33.26 SS.seek 0ml @21:48:42.19 Exhaust.select :close @21:48:42.30 SSV.dial CBV @21:48:42.34 CBV.dial SSV @21:48:43.05 Allowing 130s for 25.0ml of remaining 375.0ml @21:48:43.06 Intake.select :open @21:48:45.22 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:48:45.24 SS.reconfigure :inhaling 0.61ml/s @21:48:45.28 SS.seek 25ml @21:49:05.75 recoverPRVreagent08 @21:49:05.76 PTV.dial :mixing @21:49:05.77 PBV.dial :waste1 @21:49:06.12 delay 30 seconds @21:49:24.89 Vacuum too high -- waiting up to 40 seconds @21:49:24.90 SS.reconfigure :exhale @21:49:24.94 SS.hold @21:49:27.94 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:49:27.96 SS.reconfigure :inhaling 0.56ml/s @21:49:28.01 SS.seek 25ml @21:49:32.58 Stroke completed -- waiting for pressure to equalize ... @21:49:32.59 SS.reconfigure :exhale @21:49:32.63 SS.hold @21:49:36.09 Intake.select :close @21:49:36.14 PBV.dial between PSV and waste1 @21:49:36.24 SSV.dial :exhaust @21:49:36.49 PSV.dial :air @21:49:36.93 PS.reconfigure :fast @21:49:36.99 Exhaust.select :open @21:49:37.02 PS.seek 2.5ml @21:49:37.14 SS.seek 0ml @21:49:46.08 Exhaust.select :close @21:49:46.19 SSV.dial CBV @21:49:46.21 CBV.dial SSV @21:49:46.96 Allowing 130s for 25.0ml of remaining 350.0ml @21:49:46.97 Intake.select :open @21:49:49.12 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:49:49.14 SS.reconfigure :inhaling 0.56ml/s @21:49:49.18 SS.seek 25ml @21:50:00.10 PSV.dial :mixloop @21:50:00.55 delay 2 seconds @21:50:02.57 PSV.dial :air @21:50:03.02 delay 2 seconds @21:50:05.04 PSV.dial :mixloop @21:50:05.50 delay 2 seconds @21:50:07.51 PSV.dial :air @21:50:07.97 PS.seek 0ml @21:50:30.70 washCycleDA #1 of 3 @21:50:30.71 PSR.select :air @21:50:30.72 PSV.dial PSR @21:50:32.14 Pulling 0.5ml of PSR.wash @21:50:32.15 PSR.select :wash @21:50:32.26 PS.reconfigure :default @21:50:32.30 PS.seek 0.5ml @21:50:37.42 Stroke completed -- waiting for pressure to equalize ... @21:50:37.43 SS.reconfigure :exhale @21:50:37.47 SS.hold @21:50:38.23 delay 5 seconds @21:50:42.34 Intake.select :close @21:50:42.49 SSV.dial :exhaust @21:50:43.22 Exhaust.select :open @21:50:43.27 PSR.select :air @21:50:43.33 SS.seek 0ml @21:50:43.38 PS.seek 2ml @21:50:52.70 Exhaust.select :close @21:50:52.81 SSV.dial CBV @21:50:52.84 CBV.dial SSV @21:50:53.56 Allowing 130s for 25.0ml of remaining 325.0ml @21:50:53.58 Intake.select :open @21:50:55.73 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:50:56.25 SS.reconfigure :inhaling 0.56ml/s @21:50:56.29 SS.seek 25ml @21:50:59.44 PTV.dial :puck @21:51:00.14 PSV.dial PTV @21:51:00.86 PBV.dial :waste1 @21:51:01.28 PS.seek 1.5ml @21:51:07.19 delay 3 seconds @21:51:10.21 PBV.dial between PSV and waste1 @21:51:10.58 PS.seek 0ml @21:51:26.69 delay 2:00 @21:51:43.78 Stroke completed -- waiting for pressure to equalize ... @21:51:43.79 SS.reconfigure :exhale @21:51:43.83 SS.hold @21:51:49.37 Intake.select :close @21:51:49.51 SSV.dial :exhaust @21:51:50.23 Exhaust.select :open @21:51:50.34 SS.seek 0ml @21:51:59.36 Exhaust.select :close @21:51:59.46 SSV.dial CBV @21:51:59.49 CBV.dial SSV @21:52:00.23 Allowing 130s for 25.0ml of remaining 300.0ml @21:52:00.25 Intake.select :open @21:52:02.41 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:52:02.43 SS.reconfigure :inhaling 0.56ml/s @21:52:02.47 SS.seek 25ml @21:52:50.46 Stroke completed -- waiting for pressure to equalize ... @21:52:50.47 SS.reconfigure :exhale @21:52:50.52 SS.hold @21:52:55.38 Intake.select :close @21:52:55.53 SSV.dial :exhaust @21:52:56.26 Exhaust.select :open @21:52:56.37 SS.seek 0ml @21:53:05.35 Exhaust.select :close @21:53:05.46 SSV.dial CBV @21:53:05.50 CBV.dial SSV @21:53:06.22 Allowing 130s for 25.0ml of remaining 275.0ml @21:53:06.23 Intake.select :open @21:53:08.38 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:53:08.40 SS.reconfigure :inhaling 0.56ml/s @21:53:08.44 SS.seek 25ml @21:53:26.71 recoverPSRreagent08 @21:53:26.72 PTV.dial :puck @21:53:26.74 PBV.dial :waste1 @21:53:27.09 delay 30 seconds @21:53:56.32 Stroke completed -- waiting for pressure to equalize ... @21:53:56.33 SS.reconfigure :exhale @21:53:56.37 SS.hold @21:53:57.17 PBV.dial between PSV and waste1 @21:53:57.54 PSR.select :air @21:53:57.56 PSV.dial PSR @21:53:58.30 PS.reconfigure :fast @21:53:58.35 PS.seek 2.5ml @21:54:01.91 Intake.select :close @21:54:02.05 SSV.dial :exhaust @21:54:02.77 Exhaust.select :open @21:54:02.88 SS.seek 0ml @21:54:12.27 Exhaust.select :close @21:54:12.38 SSV.dial CBV @21:54:12.42 CBV.dial SSV @21:54:13.14 Allowing 130s for 25.0ml of remaining 250.0ml @21:54:13.15 Intake.select :open @21:54:15.30 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:54:15.32 SS.reconfigure :inhaling 0.56ml/s @21:54:15.36 SS.seek 25ml @21:54:21.34 PSV.dial PTV @21:54:22.06 delay 5 seconds @21:54:27.08 PSV.dial PSR @21:54:28.39 delay 5 seconds @21:54:33.41 PSV.dial PTV @21:54:34.12 delay 5 seconds @21:54:39.14 PSV.dial :air @21:54:39.85 PS.seek 0ml @21:55:02.53 washCycleDA #2 of 3 @21:55:02.55 PSR.select :air @21:55:02.56 PSV.dial PSR @21:55:02.62 Stroke completed -- waiting for pressure to equalize ... @21:55:02.63 SS.reconfigure :exhale @21:55:02.67 SS.hold @21:55:03.88 Pulling 0.5ml of PSR.wash @21:55:03.89 PSR.select :wash @21:55:04.00 PS.reconfigure :default @21:55:04.04 PS.seek 0.5ml @21:55:08.23 Intake.select :close @21:55:08.38 SSV.dial :exhaust @21:55:09.10 Exhaust.select :open @21:55:09.21 SS.seek 0ml @21:55:10.16 delay 5 seconds @21:55:15.17 PSR.select :air @21:55:15.28 PS.seek 2ml @21:55:18.49 Exhaust.select :close @21:55:18.60 SSV.dial CBV @21:55:18.62 CBV.dial SSV @21:55:19.35 Allowing 130s for 25.0ml of remaining 225.0ml @21:55:19.36 Intake.select :open @21:55:21.52 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:55:21.54 SS.reconfigure :inhaling 0.56ml/s @21:55:21.58 SS.seek 25ml @21:55:31.25 PTV.dial :puck @21:55:31.26 PSV.dial PTV @21:55:31.95 PBV.dial :waste1 @21:55:32.37 PS.seek 1.5ml @21:55:38.28 delay 3 seconds @21:55:41.30 PBV.dial between PSV and waste1 @21:55:41.66 PS.seek 0ml @21:55:57.68 delay 2:00 @21:56:09.08 Stroke completed -- waiting for pressure to equalize ... @21:56:09.09 SS.reconfigure :exhale @21:56:09.14 SS.hold @21:56:15.53 Intake.select :close @21:56:15.67 SSV.dial :exhaust @21:56:16.40 Exhaust.select :open @21:56:16.51 SS.seek 0ml @21:56:25.75 Exhaust.select :close @21:56:25.86 SSV.dial CBV @21:56:25.89 CBV.dial SSV @21:56:26.63 Allowing 130s for 25.0ml of remaining 200.0ml @21:56:26.64 Intake.select :open @21:56:28.80 Intake @15.2psia, vacuum from 9.7 to 12.7psi @21:56:28.82 SS.reconfigure :inhaling 0.56ml/s @21:56:28.86 SS.seek 25ml @21:57:16.30 Stroke completed -- waiting for pressure to equalize ... @21:57:16.31 SS.reconfigure :exhale @21:57:16.35 SS.hold @21:57:22.75 Intake.select :close @21:57:22.89 SSV.dial :exhaust @21:57:23.63 Exhaust.select :open @21:57:23.74 SS.seek 0ml @21:57:33.02 Exhaust.select :close @21:57:33.12 SSV.dial CBV @21:57:33.15 CBV.dial SSV @21:57:33.89 Allowing 130s for 25.0ml of remaining 175.0ml @21:57:33.91 Intake.select :open @21:57:36.57 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:57:36.59 SS.reconfigure :inhaling 0.56ml/s @21:57:36.63 SS.seek 25ml @21:57:57.71 recoverPSRreagent08 @21:57:57.72 PTV.dial :puck @21:57:57.73 PBV.dial :waste1 @21:57:58.09 delay 30 seconds @21:58:24.25 Stroke completed -- waiting for pressure to equalize ... @21:58:24.26 SS.reconfigure :exhale @21:58:24.30 SS.hold @21:58:28.10 PBV.dial between PSV and waste1 @21:58:28.46 PSR.select :air @21:58:28.48 PSV.dial PSR @21:58:29.20 PS.reconfigure :fast @21:58:29.24 PS.seek 2.5ml @21:58:30.70 Intake.select :close @21:58:30.85 SSV.dial :exhaust @21:58:31.56 Exhaust.select :open @21:58:31.67 SS.seek 0ml @21:58:40.59 Exhaust.select :close @21:58:40.70 SSV.dial CBV @21:58:40.74 CBV.dial SSV @21:58:41.45 Allowing 130s for 25.0ml of remaining 150.0ml @21:58:41.47 Intake.select :open @21:58:43.62 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:58:43.64 SS.reconfigure :inhaling 0.56ml/s @21:58:43.68 SS.seek 25ml @21:58:52.21 PSV.dial PTV @21:58:52.93 delay 5 seconds @21:58:57.95 PSV.dial PSR @21:58:58.66 delay 5 seconds @21:59:03.68 PSV.dial PTV @21:59:04.51 delay 5 seconds @21:59:09.53 PSV.dial :air @21:59:10.26 PS.seek 0ml @21:59:32.07 Stroke completed -- waiting for pressure to equalize ... @21:59:32.09 SS.reconfigure :exhale @21:59:32.13 SS.hold @21:59:32.87 washCycleDA #3 of 3 @21:59:32.88 PSR.select :air @21:59:32.89 PSV.dial PSR @21:59:34.20 Pulling 0.5ml of PSR.wash @21:59:34.21 PSR.select :wash @21:59:34.32 PS.reconfigure :default @21:59:34.37 PS.seek 0.5ml @21:59:38.66 Intake.select :close @21:59:38.81 SSV.dial :exhaust @21:59:39.54 Exhaust.select :open @21:59:39.66 SS.seek 0ml @21:59:40.21 delay 5 seconds @21:59:45.23 PSR.select :air @21:59:45.33 PS.seek 2ml @21:59:48.93 Exhaust.select :close @21:59:49.04 SSV.dial CBV @21:59:49.08 CBV.dial SSV @21:59:49.80 Allowing 130s for 25.0ml of remaining 125.0ml @21:59:49.81 Intake.select :open @21:59:51.96 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:59:51.98 SS.reconfigure :inhaling 0.56ml/s @21:59:52.03 SS.seek 25ml @22:00:01.36 PTV.dial :puck @22:00:01.38 PSV.dial PTV @22:00:02.07 PBV.dial :waste1 @22:00:02.49 PS.seek 1.5ml @22:00:08.43 delay 3 seconds @22:00:11.44 PBV.dial between PSV and waste1 @22:00:11.81 PS.seek 0ml @22:00:27.86 delay 2:00 @22:00:39.63 Stroke completed -- waiting for pressure to equalize ... @22:00:39.65 SS.reconfigure :exhale @22:00:39.69 SS.hold @22:00:47.16 Intake.select :close @22:00:47.31 SSV.dial :exhaust @22:00:48.04 Exhaust.select :open @22:00:48.15 SS.seek 0ml @22:00:56.99 Exhaust.select :close @22:00:57.10 SSV.dial CBV @22:00:57.13 CBV.dial SSV @22:00:57.89 Allowing 130s for 25.0ml of remaining 100.0ml @22:00:57.90 Intake.select :open @22:01:00.07 Intake @15.1psia, vacuum from 9.6 to 12.6psi @22:01:00.08 SS.reconfigure :inhaling 0.56ml/s @22:01:00.13 SS.seek 25ml @22:01:49.52 Stroke completed -- waiting for pressure to equalize ... @22:01:49.53 SS.reconfigure :exhale @22:01:49.58 SS.hold @22:01:57.04 Intake.select :close @22:01:57.19 SSV.dial :exhaust @22:01:57.92 Exhaust.select :open @22:01:58.03 SS.seek 0ml @22:02:07.33 Exhaust.select :close @22:02:07.44 SSV.dial CBV @22:02:07.47 CBV.dial SSV @22:02:08.21 Allowing 130s for 25.0ml of remaining 75.0ml @22:02:08.23 Intake.select :open @22:02:10.38 Intake @15.1psia, vacuum from 9.6 to 12.6psi @22:02:10.40 SS.reconfigure :inhaling 0.56ml/s @22:02:10.44 SS.seek 25ml @22:02:27.89 recoverPSRreagent08 @22:02:27.90 PTV.dial :puck @22:02:27.91 PBV.dial :waste1 @22:02:28.27 delay 30 seconds @22:02:51.45 Vacuum too high -- waiting up to 40 seconds @22:02:51.47 SS.reconfigure :exhale @22:02:51.51 SS.hold @22:02:55.06 Intake @14.1psia, vacuum from 8.6 to 11.6psi @22:02:55.07 SS.reconfigure :inhaling 0.50ml/s @22:02:55.12 SS.seek 25ml @22:02:58.28 PBV.dial between PSV and waste1 @22:02:58.64 PSR.select :air @22:02:58.66 PSV.dial PSR @22:02:59.38 PS.reconfigure :fast @22:02:59.42 PS.seek 2.5ml @22:03:03.51 Stroke completed -- waiting for pressure to equalize ... @22:03:03.53 SS.reconfigure :exhale @22:03:03.57 SS.hold @22:03:08.33 Intake.select :close @22:03:08.48 SSV.dial :exhaust @22:03:09.19 Exhaust.select :open @22:03:09.30 SS.seek 0ml @22:03:18.24 Exhaust.select :close @22:03:18.35 SSV.dial CBV @22:03:18.37 CBV.dial SSV @22:03:19.09 Allowing 130s for 25.0ml of remaining 50.0ml @22:03:19.10 Intake.select :open @22:03:21.25 Intake @15.1psia, vacuum from 9.6 to 12.6psi @22:03:21.27 SS.reconfigure :inhaling 0.50ml/s @22:03:21.31 SS.seek 25ml @22:03:22.14 PSV.dial PTV @22:03:22.86 delay 5 seconds @22:03:27.88 PSV.dial PSR @22:03:28.59 delay 5 seconds @22:03:33.61 PSV.dial PTV @22:03:34.44 delay 5 seconds @22:03:39.46 PSV.dial :air @22:03:40.19 PS.seek 0ml @22:04:03.28 Gate.power :camera,:ON @22:04:03.29 Mixing DA Substrates @22:04:03.30 PSR.select :air @22:04:03.31 PSV.dial PSR @22:04:04.86 Pulling 0.25ml of PSR.sub1 @22:04:04.90 PSR.select :sub1 @22:04:05.21 PS.reconfigure :default @22:04:05.34 PS.seek 0.25ml @22:04:08.77 delay 5 seconds @22:04:13.85 Pulling 0.25ml of PSR.sub2 @22:04:13.86 PSR.select :sub2 @22:04:13.98 PS.seek 0.5ml @22:04:17.39 delay 5 seconds @22:04:18.50 Stroke completed -- waiting for pressure to equalize ... @22:04:18.52 SS.reconfigure :exhale @22:04:18.56 SS.hold @22:04:22.40 PSR.select :air @22:04:22.57 PS.seek 2ml @22:04:23.32 Intake.select :close @22:04:23.47 SSV.dial :exhaust @22:04:24.20 Exhaust.select :open @22:04:24.31 SS.seek 0ml @22:04:33.30 Exhaust.select :close @22:04:33.41 SSV.dial CBV @22:04:33.43 CBV.dial SSV @22:04:34.18 Allowing 130s for 25.0ml of remaining 25.0ml @22:04:34.19 Intake.select :open @22:04:36.35 Intake @15.1psia, vacuum from 9.6 to 12.6psi @22:04:36.36 SS.reconfigure :inhaling 0.50ml/s @22:04:36.41 SS.seek 24.95ml @22:04:38.67 PTV.dial :puck @22:04:38.69 PSV.dial PTV @22:04:39.40 PBV.dial :waste1 @22:04:39.84 PS.seek 1.5ml @22:04:45.73 delay 3 seconds @22:04:48.75 PBV.dial between PSV and waste1 @22:04:49.11 PS.seek 0ml @22:05:05.26 delay 10 seconds @22:05:15.29 recoverPSRreagent08 @22:05:15.30 PTV.dial :puck @22:05:15.31 PBV.dial :waste1 @22:05:15.67 delay 30 seconds @22:05:32.58 Stroke completed -- waiting for pressure to equalize ... @22:05:32.59 SS.reconfigure :exhale @22:05:32.63 SS.hold @22:05:39.08 Intake.select :close @22:05:39.22 SSV.dial :exhaust @22:05:39.97 Exhaust.select :open @22:05:40.08 SS.seek 0ml @22:05:45.69 PBV.dial between PSV and waste1 @22:05:46.04 PSR.select :air @22:05:46.06 PSV.dial PSR @22:05:46.91 PS.reconfigure :fast @22:05:46.95 PS.seek 2.5ml @22:05:49.39 Exhaust.select :close @22:05:49.50 Sampled 1000.0ml @22:05:49.52 Drying puck before fixing for 20:00 @22:05:49.53 CBV.dial :waste2b @22:05:50.25 delay 1:00 @22:06:09.95 PSV.dial PTV @22:06:10.65 delay 5 seconds @22:06:15.67 PSV.dial PSR @22:06:16.39 delay 5 seconds @22:06:21.40 PSV.dial PTV @22:06:22.12 delay 5 seconds @22:06:27.14 PSV.dial :air @22:06:27.86 PS.seek 0ml @22:06:50.27 CSV.dial :air @22:06:50.54 Additional air clear @22:06:50.55 PSR.select :air @22:06:50.57 PSV.dial PSR @22:06:50.73 CS.reconfigure :fast @22:06:50.78 CS.seek 10ml @22:06:51.86 Pulling 2ml of PSR.air @22:06:51.87 PSR.select :air @22:06:51.89 PS.reconfigure :default @22:06:51.93 PS.seek 2ml @22:07:13.01 delay 5 seconds @22:07:13.31 Pumping wcr puck from tube 2 up to 35psi... @22:07:13.32 CBV.dial SSV @22:07:14.61 CBV.dial between CSV and waste2b @22:07:15.52 CSV.dial between air and CTV @22:07:16.35 CS.seek 7.583ml @22:07:18.02 PTV.dial :puck @22:07:18.04 PSV.dial PTV @22:07:18.74 PBV.dial :waste1 @22:07:19.16 PS.seek 1.5ml @22:07:22.53 CTV.dial CSV,avoiding: :air @22:07:22.56 CSV.dial CTV @22:07:23.37 CS.reconfigure :pressurize @22:07:23.41 CS.seek 0ml @22:07:25.07 delay 3 seconds @22:07:28.09 PBV.dial between PSV and waste1 @22:07:28.45 delay 1 second @22:07:29.47 PSV.dial :waste1 @22:07:30.45 PS.seek 0ml @22:07:38.76 CBV.dial :waste2b @22:07:39.09 CS.reconfigure :default @22:07:39.14 CS.seek 0ml @22:07:44.15 delay 1:00 @22:07:46.56 Processing Puck Heater at 28.6C before imaging @22:07:46.57 PH.coast @22:07:46.69 Imaging da puck in Processing Clamp while storing it to tube 1 @22:07:46.85 SC.reconfigure :tight @22:07:46.89 SC.seek 1 @22:07:50.55 SC.configure :limp @22:07:50.74 Elbow.reconfigure :tight @22:07:50.79 Elbow.seek 1 @22:07:52.73 Forearm.reconfigure :tight @22:07:52.77 Forearm.seek 1 + 700 counts @22:07:53.91 SE.reconfigure :pick @22:07:53.96 SE.seek :beamBreak @22:07:56.49 SE.configure :default @22:07:56.64 Elbow.reconfigure :limp @22:07:56.68 Forearm.reconfigure :loose @22:07:56.72 Forearm.seek 1 @22:07:57.13 Elbow.hold @22:07:57.19 Elbow.reconfigure :tight @22:07:57.28 SE.seek 9.28 Pucks below Top Plate @22:07:57.84 Forearm.force -20 @22:07:57.88 Forearm.seek 1 + 313 counts @22:07:58.21 Forearm.reconfigure :tight @22:07:58.26 Forearm.seek 1 + 700 counts @22:07:58.59 SE.seek 10.28 Pucks below Top Plate @22:07:59.14 Forearm.reconfigure :loose @22:07:59.18 Forearm.seek :retract @22:08:00.10 Elbow.seek PC @22:08:02.62 Elbow.reconfigure :loose @22:08:02.71 Forearm.seek PC @22:08:03.82 Hand.close @22:08:04.54 PC.seek :open @22:08:08.54 Forearm.seek :retract @22:08:09.71 Elbow.reconfigure :tight @22:08:09.75 Elbow.seek 1 @22:08:12.36 Forearm.reconfigure :tight @22:08:12.41 Forearm.seek 1 + 700 counts @22:08:13.40 Hand.open @22:08:14.22 SE.seek 9.93 Pucks below Top Plate @22:08:14.49 SE.reconfigure :seal @22:08:14.54 SE.seek 8.82 Pucks below Top Plate @22:08:15.09 Lights, camera, ACTION! @22:08:15.12 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22oct1322h575ml.tif @22:08:31.44 SX MX5/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 12.662 seconds /var/log/esp/da22oct1322h575ml.tif: TIFF Upload Complete @22:08:31.47 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22oct1322h575ml15s.tif @22:08:34.59 Waiting >20 seconds for da to relinquish FlushPuck @22:08:44.18 CTV.dial :air,avoiding: :intake @22:08:45.44 Fix #1 of 2 (with RNAlater) for 10:00 @22:08:45.45 CSV.dial CSR @22:08:45.95 Pulling 2ml of CSR.RNAlater @22:08:45.96 CSR.select :RNAlater @22:08:46.08 CS.seek 2ml @22:08:47.27 SX MX5/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22oct1322h575ml15s.tif: TIFF Upload Complete @22:08:47.30 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da22oct1322h575ml25s.tif @22:08:52.03 delay 5 seconds @22:08:57.05 CSR.select :air @22:08:57.16 CS.reconfigure :fast @22:08:57.21 CS.seek 10ml @22:09:13.04 SX MX5/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da22oct1322h575ml25s.tif: TIFF Upload Complete @22:09:13.63 Gate.power :camera,:OFF @22:09:13.65 DAfans.email "12.662 sec auto-exposure",Subject: "da22oct1322h575ml images!" @22:09:13.95 Elbow.seek 1 @22:09:14.12 Forearm.seek 1 + 700 counts @22:09:14.19 SE.reconfigure :gently @22:09:14.25 SE.seek 9.53 Pucks below Top Plate @22:09:14.94 Elbow.reconfigure :limp @22:09:14.99 Forearm.reconfigure :loose @22:09:15.03 Forearm.seek 1 @22:09:15.43 Elbow.hold @22:09:15.51 Pumping wcr puck from tube 2 up to 35psi... @22:09:15.54 Elbow.reconfigure :tight @22:09:15.57 CBV.dial SSV @22:09:15.66 SE.reconfigure :default @22:09:15.71 SE.seek 9.72 Pucks below Top Plate @22:09:16.07 Forearm.force -20 @22:09:16.11 Forearm.seek 1 + 320 counts @22:09:16.50 Forearm.reconfigure :tight @22:09:16.54 Forearm.seek 1 + 700 counts @22:09:16.84 SE.seek :down @22:09:16.96 CBV.dial between CSV and waste2b @22:09:17.89 CSV.dial between air and CTV @22:09:18.05 Forearm.reconfigure :loose @22:09:18.10 Forearm.seek :retract @22:09:18.47 CS.seek 7.282ml @22:09:18.88 SE.stop @22:09:19.24 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:09:19.63 Elbow.seek FlushPuck::Garage @22:09:21.85 Elbow.reconfigure :loose @22:09:22.08 Forearm.seek :garage @22:09:23.21 Hand.close @22:09:24.04 Forearm.seek :retract @22:09:25.13 Elbow.reconfigure :tight @22:09:25.24 Elbow.seek PC @22:09:25.42 CTV.dial CSV,avoiding: :air @22:09:25.48 CSV.dial CTV @22:09:26.72 Elbow.reconfigure :loose @22:09:26.98 CS.reconfigure :pressurize @22:09:27.04 Forearm.reconfigure :tight @22:09:27.28 CS.seek 0ml @22:09:27.32 Forearm.seek PC @22:09:29.13 PC.seek :closed @22:09:33.17 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.45 @22:09:33.34 Hand.open @22:09:34.33 Forearm.reconfigure :loose @22:09:34.41 Forearm.seek :retract @22:09:35.55 PSR.select :air @22:09:35.59 PSV.dial PSR @22:09:37.44 Pulling 0.5ml of PSR.flush @22:09:37.46 PSR.select :flush @22:09:37.62 PS.seek 0.5ml @22:09:43.60 delay 5 seconds @22:09:44.36 delay 10:00 @22:09:48.64 PSR.select :air @22:09:48.81 PS.seek 2.5ml @22:10:10.01 delay 1 second @22:10:11.03 PRV.dial :waste2,avoiding: :ab2 @22:10:12.88 PTV.dial :PRVmixing @22:10:14.13 PSV.dial :mixloop @22:10:15.32 PS.seek :empty @22:10:41.61 PSR.select :air @22:10:41.65 PSV.dial PSR @22:10:42.77 Pulling 0.5ml of PSR.flush @22:10:42.79 PSR.select :flush @22:10:42.97 PS.seek 0.5ml @22:10:48.91 delay 5 seconds @22:10:53.95 PSR.select :air @22:10:54.10 PS.seek 2.5ml @22:11:15.27 delay 1 second @22:11:16.31 PBV.dial :waste1 @22:11:16.71 PTV.dial :mixing @22:11:17.46 PSV.dial :mixloop @22:11:18.49 PS.seek :empty @22:11:28.46 upsync @22:11:44.69 PSV.dial PSR @22:11:45.67 PSR.select :flush @22:11:45.79 PS.seek 1ml @22:11:56.81 PSR.select :air @22:11:56.92 PS.seek 2.5ml @22:12:12.90 PSV.dial PTV @22:12:13.61 PTV.dial :puck @22:12:14.30 PBV.dial :waste1 @22:12:14.32 PS.seek 0ml @22:12:40.53 PSV.dial PSR @22:12:41.22 PSR.select :flush @22:12:41.33 PS.seek 0.5ml @22:12:47.18 PSR.select :air @22:12:47.30 PS.seek 2.5ml @22:13:08.46 PSV.dial PTV @22:13:09.17 PBV.dial PSV @22:13:09.71 PS.seek 0ml @22:13:35.93 PSV.dial PBV @22:13:36.38 PS.seek 2.5ml @22:14:02.56 PSV.dial :waste1 @22:14:03.27 PS.seek 0ml @22:14:29.46 PSV.dial PSR @22:14:29.90 PSR.select :air @22:14:29.92 PS.seek 2.5ml @22:14:56.05 PSV.dial PTV @22:14:56.76 PBV.dial PSV @22:14:56.78 PS.seek 0ml @22:15:22.93 PSV.dial PBV @22:15:23.37 PS.seek 2.5ml @22:15:49.52 PSV.dial :waste1 @22:15:50.23 PS.seek 0ml @22:16:16.37 PSV.dial PSR @22:16:16.80 PSR.select :flush @22:16:16.92 PS.seek 1ml @22:16:27.86 PSR.select :air @22:16:27.98 PS.seek 2.5ml @22:16:44.05 PSV.dial PTV @22:16:44.76 PTV.dial :puck @22:16:44.77 PBV.dial :waste1 @22:16:45.31 PS.seek 0ml @22:17:11.43 PSV.dial PSR @22:17:12.15 PSR.select :flush @22:17:12.26 PS.seek 0.5ml @22:17:18.24 PSR.select :air @22:17:18.35 PS.seek 2.5ml @22:17:39.49 PSV.dial PTV @22:17:40.21 PBV.dial PSV @22:17:40.75 PS.seek 0ml @22:18:06.96 PSV.dial PBV @22:18:07.42 PS.seek 2.5ml @22:18:33.55 PSV.dial :waste1 @22:18:34.26 PS.seek 0ml @22:19:00.42 PSV.dial PSR @22:19:00.86 PSR.select :air @22:19:00.88 PS.seek 2.5ml @22:19:27.08 PSV.dial PTV @22:19:27.78 PBV.dial PSV @22:19:27.80 PS.seek 0ml @22:19:44.39 CBV.dial :waste2b @22:19:44.70 CS.reconfigure :default @22:19:44.74 CS.seek 0ml @22:19:47.35 delay 1:00 @22:19:54.21 PSV.dial PBV @22:19:54.66 PS.seek 2.5ml @22:20:20.92 PSV.dial :waste1 @22:20:21.63 PS.seek 0ml @22:20:47.75 CTV.dial :air,avoiding: :intake @22:20:47.83 Garaging FlushPuck in Processing Clamp @22:20:47.90 Elbow.reconfigure :tight @22:20:47.95 Elbow.seek PC @22:20:48.07 Elbow.reconfigure :loose @22:20:48.17 Forearm.seek PC @22:20:48.99 Fix #2 of 2 (with RNAlater) for 10:00 @22:20:49.01 CSV.dial CSR @22:20:49.23 Hand.close @22:20:49.62 Pulling 2ml of CSR.RNAlater @22:20:49.63 CSR.select :RNAlater @22:20:49.75 CS.seek 2ml @22:20:49.89 PC.seek :open @22:20:53.85 Forearm.seek :retract @22:20:54.87 Elbow.reconfigure :tight @22:20:54.91 Elbow.seek :garage @22:20:55.69 delay 5 seconds @22:20:56.31 Elbow.reconfigure :loose @22:20:56.40 Forearm.reconfigure :tight @22:20:56.45 Forearm.seek :garage @22:20:57.62 Hand.open @22:20:58.44 Forearm.reconfigure :loose @22:20:58.49 Forearm.seek :retract @22:21:00.71 CSR.select :air @22:21:00.82 CS.reconfigure :fast @22:21:00.86 CS.seek 10ml @22:21:18.88 Pumping wcr puck from tube 2 up to 35psi... @22:21:18.89 CBV.dial SSV @22:21:20.16 CBV.dial between CSV and waste2b @22:21:21.08 CSV.dial between air and CTV @22:21:21.65 CS.seek 7.258ml @22:21:28.38 CTV.dial CSV,avoiding: :air @22:21:28.41 CSV.dial CTV @22:21:29.72 CS.reconfigure :pressurize @22:21:29.77 CS.seek 0ml @22:21:46.80 delay 10:00 @22:31:46.82 CBV.dial :waste2b @22:31:47.14 CS.reconfigure :default @22:31:47.19 CS.seek 0ml @22:31:49.74 delay 1:00 @22:32:49.77 CTV.dial :air,avoiding: :intake @22:32:51.06 Storing wcr puck in Collection Clamp to tube 1 @22:32:51.22 SC.reconfigure :tight @22:32:51.26 SC.seek 1 @22:32:51.33 SC.configure :limp @22:32:51.54 Elbow.reconfigure :tight @22:32:51.58 Elbow.seek 1 @22:32:53.41 Forearm.reconfigure :tight @22:32:53.46 Forearm.seek 1 + 700 counts @22:32:54.61 SE.reconfigure :pick @22:32:54.66 SE.seek :beamBreak @22:32:57.14 SE.configure :default @22:32:57.29 Elbow.reconfigure :limp @22:32:57.33 Forearm.reconfigure :loose @22:32:57.38 Forearm.seek 1 @22:32:57.80 Elbow.hold @22:32:57.86 Elbow.reconfigure :tight @22:32:57.95 SE.seek 10.27 Pucks below Top Plate @22:32:58.56 Forearm.force -20 @22:32:58.60 Forearm.seek 1 + 318 counts @22:32:58.91 Forearm.reconfigure :tight @22:32:58.96 Forearm.seek 1 + 700 counts @22:32:59.76 SE.seek 11.27 Pucks below Top Plate @22:33:00.34 Forearm.reconfigure :loose @22:33:00.38 Forearm.seek :retract @22:33:01.39 Elbow.seek CC @22:33:02.99 Elbow.reconfigure :loose @22:33:03.09 Forearm.seek CC @22:33:04.08 Hand.close @22:33:04.86 CC.seek :open @22:33:09.29 Forearm.seek :retract @22:33:10.36 Elbow.reconfigure :tight @22:33:10.41 Elbow.seek 1 @22:33:12.07 Elbow.reconfigure :limp @22:33:12.12 Forearm.seek 1 @22:33:13.22 Elbow.hold @22:33:13.28 Elbow.reconfigure :tight @22:33:13.33 Hand.open @22:33:14.23 Forearm.force -20 @22:33:14.27 Forearm.seek 1 + 319 counts @22:33:14.58 Forearm.reconfigure :tight @22:33:14.63 Forearm.seek 1 + 700 counts @22:33:15.04 SE.seek :down @22:33:16.16 Forearm.reconfigure :loose @22:33:16.21 Forearm.seek :retract @22:33:16.98 SE.stop @22:33:17.13
Starting finalPurge @22:33:17.14 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:33:17.32 Elbow.seek FlushPuck::Garage @22:33:19.34 Elbow.reconfigure :loose @22:33:19.43 Forearm.seek :garage @22:33:20.55 Hand.close @22:33:21.22 Forearm.seek :retract @22:33:22.32 Elbow.reconfigure :tight @22:33:22.36 Elbow.seek CC @22:33:25.13 Elbow.reconfigure :loose @22:33:25.22 Forearm.reconfigure :tight @22:33:25.27 Forearm.seek CC @22:33:26.57 CC.seek :closed @22:33:30.87 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.84 @22:33:30.94 Hand.open @22:33:31.67 Forearm.reconfigure :loose @22:33:31.71 Forearm.seek :retract @22:33:32.80 Flushing the Collection Valve::Manifold @22:33:32.91 CSV.dial CSR @22:33:33.38 Pulling 5ml of CSR.flush @22:33:33.39 CSR.select :flush @22:33:33.50 CS.seek 5ml @22:33:46.92 delay 5 seconds @22:33:51.94 CSR.select :air @22:33:52.08 CS.reconfigure :fast @22:33:52.12 CS.seek 7.5ml @22:33:58.48 CSV.dial :waste @22:33:59.72 CS.reconfigure :default @22:33:59.76 CS.seek 0ml @22:34:19.42 Flushing lower manifold's waste1 port @22:34:19.53 CSV.dial :flush @22:34:19.97 CS.reconfigure :fast @22:34:20.01 CS.seek 5ml @22:34:31.76 delay 5 seconds @22:34:36.78 CSV.dial :air @22:34:37.27 CS.seek 7ml @22:34:42.55 CSV.dial CTV @22:34:42.58
CTV.dial CSV @22:34:44.31 CBV.dial :waste1 @22:34:45.06 CS.seek 0ml @22:35:00.92 Flushing lower manifold's waste2 port @22:35:01.03 CSV.dial :flush @22:35:01.75 CS.seek 5ml @22:35:13.48 delay 5 seconds @22:35:18.50 CSV.dial :air @22:35:19.12 CS.seek 7ml @22:35:24.29 CSV.dial CTV @22:35:24.32
CTV.dial CSV @22:35:24.89 CBV.dial :waste2 @22:35:25.87 CS.seek 0ml @22:35:41.76 Flushing lower manifold's waste2b port @22:35:41.87 CSV.dial :flush @22:35:42.58 CS.seek 5ml @22:35:54.20 delay 5 seconds @22:35:59.21 CSV.dial :air @22:35:59.72 CS.seek 6.999ml @22:36:04.89 CSV.dial CTV @22:36:04.91
CTV.dial CSV @22:36:05.34 CBV.dial :waste2b @22:36:06.33 CS.seek 0ml @22:36:22.13 Disinfecting external Intake with kill @22:36:22.25 CSV.dial CSR @22:36:22.73 Pulling 8ml of CSR.kill @22:36:22.74 CSR.select :kill @22:36:22.85 CS.seek 8ml @22:36:40.92 delay 5 seconds @22:36:45.93 CSR.select :air @22:36:46.04 CS.seek 10ml @22:36:51.26 CSV.dial CBV @22:36:51.29
CBV.dial CSV @22:36:52.32 CTV.dial :intake @22:36:53.04 Intake.select :open @22:36:53.14 CS.seek 2ml @22:37:11.04 Intake.select :close @22:37:11.14 CTV.dial :air @22:37:11.85 CS.reconfigure :default @22:37:11.90 CS.seek 0ml @22:37:17.72 CSV.dial CSR @22:37:18.75 Pulling 5ml of CSR.flush CSR.select :flush @22:37:18.86 CS.seek 5ml @22:37:32.35 delay 5 seconds @22:37:37.37 CSR.select :air @22:37:37.48 CS.seek 10ml @22:37:50.97 CSV.dial CBV @22:37:51.95 CS.seek 0ml @22:38:17.94 Garaging FlushPuck in Collection Clamp @22:38:18.01 Elbow.reconfigure :tight @22:38:18.05 Elbow.seek CC @22:38:18.15 Elbow.reconfigure :loose @22:38:18.24 Forearm.seek CC @22:38:19.19 Hand.close @22:38:19.85 CC.seek :open @22:38:24.17 Forearm.seek :retract @22:38:25.27 Elbow.reconfigure :tight @22:38:25.31 Elbow.seek :garage @22:38:28.40 Elbow.reconfigure :loose @22:38:28.49 Forearm.reconfigure :tight @22:38:28.53 Forearm.seek :garage @22:38:29.65 Hand.open @22:38:30.41 Forearm.reconfigure :loose @22:38:30.45 Forearm.seek :retract @22:38:31.62 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 20:13:52 @22:38:31.66 Asleep after completing DA_WCR_101322 assays @22:38:31.68 GoodNews.email "{1 => 12,\n 2 => 21,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:32:51, 25.1C, 17% humidity, 15.7psia, 14.007V, 0.380A, 0.380A avg, 5.32W\nSampled 1000.0ml",Subject: "Asleep after completing DA_WCR_101322 assays" @22:39:07.42
Uploading Logs @22:40:18.89 upsync @21:04:39.78UTC19-Oct-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @21:04:42.40 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @21:04:46.41 Polling Can every 10:00 @21:04:46.44 Cleanly exited at 21:02:17UTC19-Oct-22 @21:04:46.48 Loading /home/esp/esp2/mission/DA_WCR_101922.rb mission @21:04:46.53 Preparing to sleep until + 00:00:05 @21:04:46.54 GoodNews.email "Starting DA_WCR_101922 w/pucks in tube 2\n{1 => 12,\n 2 => 21,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:04:46, 25.3C, 19% humidity, 15.8psia, 14.055V, 0.233A, 0.226A avg, 3.27W",Subject: "DA_WCR_101922 begun" @21:04:46.59
Asleep til + 00:00:05 @21:04:46.63 Continuing at 21:04 @21:04:46.87 Gate.power :core,:OFF @21:04:46.88 Gate.power :raw,:OFF @21:04:46.89 Shutdown @21:04:51.50
Powering core bus @21:04:51.99 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_101922.rb:14\n{1 => 12,\n 2 => 21,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:04:46, 25.3C, 19% humidity, 15.8psia, 14.055V, 0.233A, 0.226A avg, 3.27W",Subject: "Asleep until 21:04" @21:04:57.09
Gate.power :raw,:ON @21:04:58.43 CS.configure :default @21:04:58.60 PS.configure :default @21:04:58.78 SS.configure :default @21:04:59.01 CC.configure :default @21:04:59.19 PC.configure :default @21:04:59.43 Forearm.configure :loose @21:04:59.60 Elbow.configure :loose @21:04:59.80 SC.configure :loose @21:04:59.98 SE.configure :default @21:05:00.06 CH.coast @21:05:00.16 CH.configure :default @21:05:00.22 PH.coast @21:05:00.40 PH.configure :default @21:05:00.46 All dwarf controllers running firmware version 3.85 @21:05:00.62 Valve.reset @21:05:01.06 Intake.select :close @21:05:01.16 Exhaust.select :close @21:05:01.20 CTV.dial :air @21:05:01.40 PSV.dial :air @21:05:01.46 CSV.dial :waste1 @21:05:01.65
Forearm.reconfigure :loose @21:05:01.82 Forearm.home.seek :retract @21:05:02.00 SSV.dial :spit,avoiding: :exhaust @21:05:02.52 PS.reconfigure :default @21:05:02.63 PS.home.seek 0ml @21:05:02.74 Hand.close @21:05:02.80
Elbow.jog 1500 @21:05:03.07 CS.reconfigure :fast @21:05:03.13 SS.reconfigure :default @21:05:03.27 CS.home.seek 0ml @21:05:03.33 SS.home.seek 0ml @21:05:03.56
Elbow.reconfigure :loose @21:05:03.66 Elbow.home.seek :clear @21:05:08.19 SE.reconfigure :default @21:05:08.29 SE.home.seek :down @21:05:08.92 SC.reconfigure :tight @21:05:09.00 SC.home.seek :initial @21:05:11.36 PS.stop @21:05:39.09 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @21:05:39.11
Retry #1 of 3 @21:05:39.23 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @21:05:43.49 SC.configure :limp @21:05:43.57 Forearm.seek :retract @21:05:43.69 Elbow.seek :hammer @21:05:44.99 Forearm.reconfigure :tight @21:05:45.05 Forearm.seek retracted @21:05:45.82 PC.reconfigure :default @21:05:45.88
CC.reconfigure :default @21:05:46.00 PC.home.seek :open @21:05:46.07
CC.home.seek :open @21:05:49.82 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @21:05:49.86 Notice.email "At 21:05:49 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_101922.rb:14\n{1 => 12,\n 2 => 21,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:05:49, 25.3C, 19% humidity, 15.8psia, 14.036V, 0.245A, 0.245A avg, 3.44W",Subject: "Started DA_WCR_101922 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @21:05:49.90
Starting initialPurge @21:05:49.94 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:05:50.05 Hand.open @21:05:51.20 Elbow.reconfigure :tight @21:05:51.27 Elbow.seek FlushPuck::Garage @21:05:53.25 Elbow.reconfigure :loose @21:05:53.45 Forearm.reconfigure :loose @21:05:53.53 Forearm.seek :garage @21:05:54.65 Hand.close @21:05:55.67 Forearm.seek :retract @21:05:56.82 Elbow.reconfigure :tight @21:05:56.90 Elbow.seek CC @21:05:59.80 Elbow.reconfigure :loose @21:05:59.98 Forearm.reconfigure :tight @21:06:00.04 Forearm.seek CC @21:06:01.77 CC.seek :closed @21:06:06.10 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.86 @21:06:06.28 Hand.open @21:06:07.11 Forearm.reconfigure :loose @21:06:07.17 Forearm.seek :retract @21:06:08.24 Flushing the Collection Valve::Manifold @21:06:08.48 CSV.dial CSR @21:06:09.86 Pulling 4.999ml of CSR.flush @21:06:09.88 CSR.select :flush @21:06:10.11 CS.reconfigure :default @21:06:10.21 CS.seek 5ml @21:06:23.78 delay 5 seconds @21:06:28.79 CSR.select :air @21:06:29.05 CS.reconfigure :fast @21:06:29.13 CS.seek 7.501ml @21:06:35.48 CSV.dial :waste @21:06:36.78 CS.reconfigure :default @21:06:36.89 CS.seek 0ml @21:06:43.98 upsync @21:06:56.62
Purging external Intake @21:06:56.73 CSV.dial :flush @21:06:57.18 CS.reconfigure :fast @21:06:57.23 CS.seek 10ml @21:07:19.84 delay 5 seconds @21:07:24.86 CSV.dial CBV @21:07:24.89
CBV.dial CSV @21:07:25.62 CTV.dial :intake @21:07:26.32 Intake.select :open @21:07:26.42 CS.seek 0ml @21:07:48.86 Intake.select :close @21:07:48.97 CTV.dial :air @21:07:49.68 Flushing upper manifold @21:07:49.79 CSV.dial :flush @21:07:50.51 CS.seek 5ml @21:08:02.21 delay 5 seconds @21:08:07.23 CSV.dial :air @21:08:08.38 CS.seek 7ml @21:08:13.52 CSV.dial CBV @21:08:13.55
CBV.dial CSV @21:08:14.65 CTV.dial :air @21:08:14.67 CS.seek 0ml @21:08:30.71 delay 7 seconds @21:08:37.74 CS.seek 3ml @21:08:45.05 CSV.dial :waste @21:08:45.59 CS.seek 0ml @21:08:53.09 CSV.dial :flush @21:08:53.54 CS.seek 3ml @21:09:00.89 delay 5 seconds @21:09:05.91 CSV.dial :air @21:09:06.41 CS.seek 5ml @21:09:11.65 CSV.dial :air @21:09:11.67 CS.seek 0ml @21:09:23.39 delay 7 seconds @21:09:30.42 CS.seek 3ml @21:09:37.74 CSV.dial :waste @21:09:38.45 CS.seek 0ml @21:09:45.80 Garaging FlushPuck in Collection Clamp @21:09:45.87 Elbow.reconfigure :tight @21:09:45.91 Elbow.seek CC @21:09:46.02 Elbow.reconfigure :loose @21:09:46.11 Forearm.seek CC @21:09:47.15 Hand.close @21:09:47.70 CC.seek :open @21:09:52.04 Forearm.seek :retract @21:09:53.08 Elbow.reconfigure :tight @21:09:53.12 Elbow.seek :garage @21:09:56.03 Elbow.reconfigure :loose @21:09:56.12 Forearm.reconfigure :tight @21:09:56.16 Forearm.seek :garage @21:09:57.26 Hand.open @21:09:58.35 Forearm.reconfigure :loose @21:09:58.46 Forearm.seek :retract @21:09:59.56 da sampling at most 1000ml, wcr at most 1000ml @21:09:59.59 DAfans.email "Started da in tube 2 at 21:09:59UTC19-Oct-22\n{1 => 12,\n 2 => 21,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:05:49, 25.3C, 19% humidity, 15.8psia, 14.036V, 0.245A, 0.245A avg, 3.44W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @21:09:59.61
Loading sh1 puck from tube 2 into Collection Clamp @21:09:59.86 SC.reconfigure :tight @21:09:59.90 SC.seek 2 @21:10:12.90 SC.configure :limp @21:10:13.34 Elbow.reconfigure :tight @21:10:13.42 Elbow.seek 2 @21:10:15.68 Forearm.reconfigure :tight @21:10:15.76 Forearm.seek 2 + 700 counts @21:10:16.99 SE.reconfigure :pick @21:10:17.09 SE.seek :beamBreak @21:10:18.99 SE.configure :default @21:10:19.29 Elbow.seek 2 @21:10:19.88 Forearm.seek 2 + 300 counts @21:10:20.21 Forearm.reconfigure :loose @21:10:20.28 Hand.close @21:10:21.00 SE.seek :down @21:10:21.40
Forearm.seek :retract @21:10:22.42 Elbow.seek CC @21:10:22.62 SE.stop @21:10:24.28
Elbow.reconfigure :loose @21:10:24.46 Forearm.reconfigure :tight @21:10:24.56 Forearm.seek CC @21:10:26.16 CC.seek :closed @21:10:30.60 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.75 @21:10:30.62 Hand.open @21:10:31.46 Forearm.reconfigure :loose @21:10:31.54 Forearm.seek :retract @21:10:32.67 collectSample 1000ml w/bubble point of 24psi @21:10:32.69 Priming 7ml @21:10:32.71 Intake.select :close @21:10:32.73 Exhaust.select :close @21:10:32.75 SS.hold @21:10:33.16 Calibrating pressure and temperature sensors @21:10:33.22 CTV.dial :air @21:10:33.24 SSV.dial :spit,avoiding: :exhaust @21:10:33.28 SS.reconfigure :fast @21:10:33.36 SS.seek 5ml @21:10:36.33 SSV.dial CBV @21:10:36.41
CBV.dial SSV @21:10:37.69 delay 3.8 seconds @21:10:41.73 SSV.dial :spit @21:10:43.03 SS.seek 0ml @21:10:45.81 SSV.dial CBV @21:10:47.11 CTV.dial :intake @21:10:47.90 SSV.dial CBV @21:10:47.96
CBV.dial SSV @21:10:47.98 Allowing 40s for 7.0ml of remaining 7.0ml @21:10:48.00 Intake.select :open @21:10:50.25 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:10:50.27 SS.reconfigure :inhaling 2.14ml/s @21:10:50.35 SS.seek 7ml @21:10:57.39 Stroke completed -- waiting for pressure to equalize ... @21:10:57.41 SS.reconfigure :exhale @21:10:57.49 SS.hold @21:11:00.43 Intake.select :close @21:11:00.67 SSV.dial :exhaust @21:11:01.42 Exhaust.select :open @21:11:01.60 SS.seek 0ml @21:11:05.56 Exhaust.select :close @21:11:05.71 Sampling 1000.0ml @21:11:05.72 Intake.select :close @21:11:05.74 Exhaust.select :close @21:11:05.78 SS.reconfigure :default @21:11:05.84 SS.hold @21:11:06.25 CTV.dial :intake @21:11:06.29 SSV.dial CBV @21:11:06.35
CBV.dial SSV @21:11:07.14 Allowing 130s for 25.0ml of remaining 1000.0ml @21:11:07.16 Intake.select :open @21:11:09.39 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:11:09.41 SS.reconfigure :inhaling 2.14ml/s @21:11:09.56 SS.seek 25ml @21:11:14.93 Vacuum too high -- waiting up to 40 seconds @21:11:14.95 SS.reconfigure :exhale @21:11:15.01 SS.hold @21:11:15.80 Intake @12.1psia, vacuum from 6.6 to 9.6psi @21:11:15.84 SS.reconfigure :inhaling 1.93ml/s @21:11:15.92 SS.seek 25ml @21:11:18.55 Vacuum too high -- waiting up to 40 seconds @21:11:18.57 SS.reconfigure :exhale @21:11:18.64 SS.hold @21:11:20.16 Intake @14.2psia, vacuum from 8.7 to 11.7psi @21:11:20.20 SS.reconfigure :inhaling 1.74ml/s @21:11:20.28 SS.seek 25ml @21:11:23.46 Vacuum too high -- waiting up to 40 seconds @21:11:23.48 SS.reconfigure :exhale @21:11:23.54 SS.hold @21:11:24.20 Intake @12.2psia, vacuum from 6.7 to 9.7psi @21:11:24.24 SS.reconfigure :inhaling 1.57ml/s @21:11:24.30 SS.seek 25ml @21:11:27.76 Vacuum too high -- waiting up to 40 seconds @21:11:27.78 SS.reconfigure :exhale @21:11:27.86 SS.hold @21:11:29.49 Intake @15.2psia, vacuum from 9.7 to 12.7psi @21:11:29.53 SS.reconfigure :inhaling 1.41ml/s @21:11:29.61 SS.seek 25ml @21:11:34.21 Vacuum too high -- waiting up to 40 seconds @21:11:34.23 SS.reconfigure :exhale @21:11:34.31 SS.hold @21:11:34.94 Intake @12.2psia, vacuum from 6.7 to 9.7psi @21:11:34.98 SS.reconfigure :inhaling 1.27ml/s @21:11:35.08 SS.seek 25ml @21:11:39.65 Vacuum too high -- waiting up to 40 seconds @21:11:39.67 SS.reconfigure :exhale @21:11:39.76 SS.hold @21:11:41.06 Intake @14.0psia, vacuum from 8.5 to 11.5psi @21:11:41.08 SS.reconfigure :inhaling 1.14ml/s @21:11:41.18 SS.seek 25ml @21:11:43.09 Stroke completed -- waiting for pressure to equalize ... @21:11:43.11 SS.reconfigure :exhale @21:11:43.19 SS.hold @21:11:43.93 Intake.select :close @21:11:44.17 SSV.dial :exhaust @21:11:44.93 Exhaust.select :open @21:11:45.09 SS.seek 0ml @21:11:54.12 Exhaust.select :close @21:11:54.27 SSV.dial CBV @21:11:54.31
CBV.dial SSV @21:11:55.09 Allowing 130s for 25.0ml of remaining 975.0ml @21:11:55.11 Intake.select :open @21:11:57.30 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:11:57.32 SS.reconfigure :inhaling 1.14ml/s @21:11:57.37 SS.seek 25ml @21:11:57.50 upsync @21:12:08.53
Vacuum too high -- waiting up to 40 seconds @21:12:08.54 SS.reconfigure :exhale @21:12:08.58 SS.hold @21:12:08.96 Intake @11.6psia, vacuum from 6.1 to 9.1psi @21:12:08.98 SS.reconfigure :inhaling 1.03ml/s @21:12:09.02 SS.seek 25ml @21:12:19.47 Vacuum too high -- waiting up to 40 seconds @21:12:19.48 SS.reconfigure :exhale @21:12:19.52 SS.hold @21:12:20.79 Intake @13.8psia, vacuum from 8.3 to 11.3psi @21:12:20.81 SS.reconfigure :inhaling 0.93ml/s @21:12:20.85 SS.seek 25ml @21:12:28.07 Stroke completed -- waiting for pressure to equalize ... @21:12:28.08 SS.reconfigure :exhale @21:12:28.12 SS.hold @21:12:29.66 Intake.select :close @21:12:29.80 SSV.dial :exhaust @21:12:30.51 Exhaust.select :open @21:12:30.61 SS.seek 0ml @21:12:39.40 Exhaust.select :close @21:12:39.50 SSV.dial CBV @21:12:39.53
CBV.dial SSV @21:12:40.26 Allowing 130s for 25.0ml of remaining 950.0ml @21:12:40.27 Intake.select :open @21:12:42.42 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:12:42.43 SS.reconfigure :inhaling 0.93ml/s @21:12:42.48 SS.seek 25ml @21:13:12.36 Stroke completed -- waiting for pressure to equalize ... @21:13:12.37 SS.reconfigure :exhale @21:13:12.41 SS.hold @21:13:14.35 Intake.select :close @21:13:14.49 SSV.dial :exhaust @21:13:15.21 Exhaust.select :open @21:13:15.32 SS.seek 0ml @21:13:24.11 Exhaust.select :close @21:13:24.21 SSV.dial CBV @21:13:24.24
CBV.dial SSV @21:13:24.97 Allowing 130s for 25.0ml of remaining 925.0ml @21:13:24.98 Intake.select :open @21:13:27.14 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:13:27.15 SS.reconfigure :inhaling 0.93ml/s @21:13:27.19 SS.seek 25ml @21:13:57.09 Stroke completed -- waiting for pressure to equalize ... @21:13:57.10 SS.reconfigure :exhale @21:13:57.15 SS.hold @21:13:59.53 Intake.select :close @21:13:59.67 SSV.dial :exhaust @21:14:00.39 Exhaust.select :open @21:14:00.50 SS.seek 0ml @21:14:09.78 Exhaust.select :close @21:14:09.88 SSV.dial CBV @21:14:09.91
CBV.dial SSV @21:14:10.64 Allowing 130s for 25.0ml of remaining 900.0ml @21:14:10.65 Intake.select :open @21:14:12.81 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:14:12.82 SS.reconfigure :inhaling 0.93ml/s @21:14:12.86 SS.seek 25ml @21:14:35.59 Vacuum too high -- waiting up to 40 seconds @21:14:35.60 SS.reconfigure :exhale @21:14:35.64 SS.hold @21:14:36.02 Intake @12.5psia, vacuum from 7.0 to 10.0psi @21:14:36.04 SS.reconfigure :inhaling 0.84ml/s @21:14:36.08 SS.seek 25ml @21:14:43.44 Stroke completed -- waiting for pressure to equalize ... @21:14:43.45 SS.reconfigure :exhale @21:14:43.49 SS.hold @21:14:45.12 Intake.select :close @21:14:45.26 SSV.dial :exhaust @21:14:45.97 Exhaust.select :open @21:14:46.55 SS.seek 0ml @21:14:55.41 Exhaust.select :close @21:14:55.51 SSV.dial CBV @21:14:55.56
CBV.dial SSV @21:14:56.25 Allowing 130s for 25.0ml of remaining 875.0ml @21:14:56.26 Intake.select :open @21:14:58.42 Intake @14.2psia, vacuum from 8.7 to 11.7psi @21:14:58.43 SS.reconfigure :inhaling 0.84ml/s @21:14:58.48 SS.seek 25ml @21:15:30.97 Stroke completed -- waiting for pressure to equalize ... @21:15:30.98 SS.reconfigure :exhale @21:15:31.02 SS.hold @21:15:33.05 Intake.select :close @21:15:33.19 SSV.dial :exhaust @21:15:33.91 Exhaust.select :open @21:15:34.02 SS.seek 0ml @21:15:43.22 Exhaust.select :close @21:15:43.32 SSV.dial CBV @21:15:43.37
CBV.dial SSV @21:15:44.06 Allowing 130s for 25.0ml of remaining 850.0ml @21:15:44.07 Intake.select :open @21:15:46.23 Intake @14.1psia, vacuum from 8.6 to 11.6psi @21:15:46.24 SS.reconfigure :inhaling 0.84ml/s @21:15:46.28 SS.seek 25ml @21:16:17.75 Stroke completed -- waiting for pressure to equalize ... @21:16:17.76 SS.reconfigure :exhale @21:16:17.80 SS.hold @21:16:20.19 Intake.select :close @21:16:20.32 SSV.dial :exhaust @21:16:21.05 Exhaust.select :open @21:16:21.15 SS.seek 0ml @21:16:30.43 Exhaust.select :close @21:16:30.54 SSV.dial CBV @21:16:30.56
CBV.dial SSV @21:16:31.30 Allowing 130s for 25.0ml of remaining 825.0ml @21:16:31.31 Intake.select :open @21:16:33.46 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:16:33.47 SS.reconfigure :inhaling 0.84ml/s @21:16:33.52 SS.seek 25ml @21:17:05.14 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @21:17:05.19 SS.hold @21:17:08.09 Intake.select :close @21:17:08.23 SSV.dial :exhaust @21:17:08.93 Exhaust.select :open @21:17:09.04 SS.seek 0ml @21:17:17.84 Exhaust.select :close @21:17:17.94 SSV.dial CBV @21:17:17.98
CBV.dial SSV @21:17:18.70 Allowing 130s for 25.0ml of remaining 800.0ml @21:17:18.71 Intake.select :open @21:17:20.86 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:17:20.88 SS.reconfigure :inhaling 0.84ml/s @21:17:20.92 SS.seek 25ml @21:17:50.83 Vacuum too high -- waiting up to 40 seconds @21:17:50.84 SS.reconfigure :exhale @21:17:50.88 SS.hold @21:17:52.51 Intake @13.0psia, vacuum from 7.5 to 10.5psi @21:17:52.52 SS.reconfigure :inhaling 0.76ml/s @21:17:52.56 SS.seek 25ml @21:17:55.65 Stroke completed -- waiting for pressure to equalize ... @21:17:55.66 SS.reconfigure :exhale @21:17:55.70 SS.hold @21:17:57.65 Intake.select :close @21:17:57.78 SSV.dial :exhaust @21:17:58.50 Exhaust.select :open @21:17:58.60 SS.seek 0ml @21:18:07.51 Exhaust.select :close @21:18:07.61 SSV.dial CBV @21:18:07.65
CBV.dial SSV @21:18:08.37 Allowing 130s for 25.0ml of remaining 775.0ml @21:18:08.38 Intake.select :open @21:18:10.53 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:18:10.55 SS.reconfigure :inhaling 0.76ml/s @21:18:10.59 SS.seek 25ml @21:18:46.30 Stroke completed -- waiting for pressure to equalize ... @21:18:46.31 SS.reconfigure :exhale @21:18:46.35 SS.hold @21:18:49.24 Intake.select :close @21:18:49.38 SSV.dial :exhaust @21:18:50.10 Exhaust.select :open @21:18:50.21 SS.seek 0ml @21:18:59.62 Exhaust.select :close @21:18:59.72 SSV.dial CBV @21:18:59.76
CBV.dial SSV @21:19:00.46 Allowing 130s for 25.0ml of remaining 750.0ml @21:19:00.47 Intake.select :open @21:19:02.62 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:19:02.64 SS.reconfigure :inhaling 0.76ml/s @21:19:02.68 SS.seek 25ml @21:19:37.83 Stroke completed -- waiting for pressure to equalize ... @21:19:37.84 SS.reconfigure :exhale @21:19:37.88 SS.hold @21:19:40.85 Intake.select :close @21:19:40.98 SSV.dial :exhaust @21:19:41.69 Exhaust.select :open @21:19:41.80 SS.seek 0ml @21:19:51.18 Exhaust.select :close @21:19:51.28 SSV.dial CBV @21:19:51.33
CBV.dial SSV @21:19:52.12 Allowing 130s for 25.0ml of remaining 725.0ml @21:19:52.13 Intake.select :open @21:19:54.28 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:19:54.30 SS.reconfigure :inhaling 0.76ml/s @21:19:54.34 SS.seek 25ml @21:20:18.42 Vacuum too high -- waiting up to 40 seconds @21:20:18.43 SS.reconfigure :exhale @21:20:18.47 SS.hold @21:20:20.54 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:20:20.55 SS.reconfigure :inhaling 0.69ml/s @21:20:20.59 SS.seek 25ml @21:20:32.80 Stroke completed -- waiting for pressure to equalize ... @21:20:32.81 SS.reconfigure :exhale @21:20:32.85 SS.hold @21:20:36.44 Intake.select :close @21:20:36.58 SSV.dial :exhaust @21:20:37.30 Exhaust.select :open @21:20:37.41 SS.seek 0ml @21:20:46.72 Exhaust.select :close @21:20:46.83 SSV.dial CBV @21:20:46.85
CBV.dial SSV @21:20:47.58 Allowing 130s for 25.0ml of remaining 700.0ml @21:20:47.59 Intake.select :open @21:20:49.74 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:20:49.76 SS.reconfigure :inhaling 0.69ml/s @21:20:49.80 SS.seek 25ml @21:21:28.17 Stroke completed -- waiting for pressure to equalize ... @21:21:28.18 SS.reconfigure :exhale @21:21:28.23 SS.hold @21:21:32.98 Intake.select :close @21:21:33.11 SSV.dial :exhaust @21:21:33.82 Exhaust.select :open @21:21:33.92 SS.seek 0ml @21:21:42.92 Exhaust.select :close @21:21:43.03 SSV.dial CBV @21:21:43.05
CBV.dial SSV @21:21:43.79 Allowing 130s for 25.0ml of remaining 675.0ml @21:21:43.80 Intake.select :open @21:21:45.95 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:21:45.96 SS.reconfigure :inhaling 0.69ml/s @21:21:46.00 SS.seek 25ml @21:22:09.95 Vacuum too high -- waiting up to 40 seconds @21:22:09.96 SS.reconfigure :exhale @21:22:10.00 SS.hold @21:22:12.03 Intake @14.3psia, vacuum from 8.8 to 11.8psi @21:22:12.04 SS.reconfigure :inhaling 0.61ml/s @21:22:12.08 SS.seek 25ml @21:22:29.67 Stroke completed -- waiting for pressure to equalize ... @21:22:29.68 SS.reconfigure :exhale @21:22:29.72 SS.hold @21:22:33.26 Intake.select :close @21:22:33.39 SSV.dial :exhaust @21:22:34.11 Exhaust.select :open @21:22:34.22 SS.seek 0ml @21:22:43.56 Exhaust.select :close @21:22:43.67 SSV.dial CBV @21:22:43.69
CBV.dial SSV @21:22:44.42 Allowing 130s for 25.0ml of remaining 650.0ml @21:22:44.43 Intake.select :open @21:22:46.58 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:22:46.60 SS.reconfigure :inhaling 0.61ml/s @21:22:46.64 SS.seek 25ml @21:23:24.16 Vacuum too high -- waiting up to 40 seconds @21:23:24.17 SS.reconfigure :exhale @21:23:24.21 SS.hold @21:23:27.18 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:23:27.20 SS.reconfigure :inhaling 0.56ml/s @21:23:27.24 SS.seek 25ml @21:23:33.07 Stroke completed -- waiting for pressure to equalize ... @21:23:33.08 SS.reconfigure :exhale @21:23:33.13 SS.hold @21:23:36.59 Intake.select :close @21:23:36.72 SSV.dial :exhaust @21:23:37.45 Exhaust.select :open @21:23:37.56 SS.seek 0ml @21:23:46.72 Exhaust.select :close @21:23:46.82 SSV.dial CBV @21:23:47.22
CBV.dial SSV @21:23:48.05 Allowing 130s for 25.0ml of remaining 625.0ml @21:23:48.06 Intake.select :open @21:23:50.21 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:23:50.23 SS.reconfigure :inhaling 0.56ml/s @21:23:50.27 SS.seek 25ml @21:24:37.49 Stroke completed -- waiting for pressure to equalize ... @21:24:37.50 SS.reconfigure :exhale @21:24:37.55 SS.hold @21:24:42.44 Intake.select :close @21:24:42.58 SSV.dial :exhaust @21:24:43.29 Exhaust.select :open @21:24:43.40 SS.seek 0ml @21:24:52.28 Exhaust.select :close @21:24:52.38 SSV.dial CBV @21:24:52.41
CBV.dial SSV @21:24:53.14 Allowing 130s for 25.0ml of remaining 600.0ml @21:24:53.15 Intake.select :open @21:24:55.30 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:24:55.32 SS.reconfigure :inhaling 0.56ml/s @21:24:55.36 SS.seek 25ml @21:25:41.31 Vacuum too high -- waiting up to 40 seconds @21:25:41.32 SS.reconfigure :exhale @21:25:41.36 SS.hold @21:25:44.94 Intake @14.3psia, vacuum from 8.8 to 11.8psi @21:25:44.96 SS.reconfigure :inhaling 0.50ml/s @21:25:45.00 SS.seek 25ml @21:25:47.45 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @21:25:47.50 SS.hold @21:25:50.38 Intake.select :close @21:25:50.52 SSV.dial :exhaust @21:25:51.24 Exhaust.select :open @21:25:51.35 SS.seek 0ml @21:26:00.70 Exhaust.select :close @21:26:00.80 SSV.dial CBV @21:26:00.82
CBV.dial SSV @21:26:01.56 Allowing 130s for 25.0ml of remaining 575.0ml @21:26:01.57 Intake.select :open @21:26:03.72 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:26:03.73 SS.reconfigure :inhaling 0.50ml/s @21:26:03.78 SS.seek 25ml @21:26:56.86 Stroke completed -- waiting for pressure to equalize ... @21:26:56.87 SS.reconfigure :exhale @21:26:56.92 SS.hold @21:27:02.53 Intake.select :close @21:27:02.66 SSV.dial :exhaust @21:27:03.38 Exhaust.select :open @21:27:03.49 SS.seek 0ml @21:27:12.46 Exhaust.select :close @21:27:12.56 SSV.dial CBV @21:27:12.61
CBV.dial SSV @21:27:13.30 Allowing 130s for 25.0ml of remaining 550.0ml @21:27:13.31 Intake.select :open @21:27:15.47 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:27:15.48 SS.reconfigure :inhaling 0.50ml/s @21:27:15.52 SS.seek 25ml @21:28:10.96 Stroke completed -- waiting for pressure to equalize ... @21:28:10.97 SS.reconfigure :exhale @21:28:11.01 SS.hold @21:28:18.35 Intake.select :close @21:28:18.49 SSV.dial :exhaust @21:28:19.21 Exhaust.select :open @21:28:19.31 SS.seek 0ml @21:28:28.60 Exhaust.select :close @21:28:29.16 SSV.dial CBV @21:28:29.19
CBV.dial SSV @21:28:29.93 Allowing 130s for 25.0ml of remaining 525.0ml @21:28:29.94 Intake.select :open @21:28:32.09 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:28:32.11 SS.reconfigure :inhaling 0.50ml/s @21:28:32.15 SS.seek 25ml @21:29:13.31 Vacuum too high -- waiting up to 40 seconds @21:29:13.32 SS.reconfigure :exhale @21:29:13.36 SS.hold @21:29:17.51 Intake @15.3psia, vacuum from 9.8 to 12.8psi @21:29:17.52 SS.reconfigure :inhaling 0.46ml/s @21:29:17.57 SS.seek 25ml @21:29:32.59 Stroke completed -- waiting for pressure to equalize ... @21:29:32.60 SS.reconfigure :exhale @21:29:32.64 SS.hold @21:29:38.27 Intake.select :close @21:29:38.40 SSV.dial :exhaust @21:29:39.11 Exhaust.select :open @21:29:39.22 SS.seek 0ml @21:29:48.22 Exhaust.select :close @21:29:48.32 SSV.dial CBV @21:29:48.37
CBV.dial SSV @21:29:49.06 Allowing 130s for 25.0ml of remaining 500.0ml @21:29:49.07 Intake.select :open @21:29:51.22 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:29:51.24 SS.reconfigure :inhaling 0.46ml/s @21:29:51.28 SS.seek 25ml @21:30:51.11 Vacuum too high -- waiting up to 40 seconds @21:30:51.12 SS.reconfigure :exhale @21:30:51.17 SS.hold @21:30:56.67 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:30:56.69 SS.reconfigure :inhaling 0.41ml/s @21:30:56.73 SS.seek 25ml @21:30:59.45 Stroke completed -- waiting for pressure to equalize ... @21:30:59.46 SS.reconfigure :exhale @21:30:59.50 SS.hold @21:31:02.94 Intake.select :close @21:31:03.08 SSV.dial :exhaust @21:31:03.81 Exhaust.select :open @21:31:03.92 SS.seek 0ml @21:31:13.48 Exhaust.select :close @21:31:13.58 SSV.dial CBV @21:31:13.61
CBV.dial SSV @21:31:14.34 Allowing 130s for 25.0ml of remaining 475.0ml @21:31:14.35 Intake.select :open @21:31:16.50 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:31:16.52 SS.reconfigure :inhaling 0.41ml/s @21:31:16.56 SS.seek 25ml @21:32:25.21 Stroke completed -- waiting for pressure to equalize ... @21:32:25.22 SS.reconfigure :exhale @21:32:25.26 SS.hold @21:32:33.70 Intake.select :close @21:32:33.84 SSV.dial :exhaust @21:32:34.54 Exhaust.select :open @21:32:34.65 SS.seek 0ml @21:32:43.51 Exhaust.select :close @21:32:43.61 SSV.dial CBV @21:32:43.65
CBV.dial SSV @21:32:44.35 Allowing 130s for 25.0ml of remaining 450.0ml @21:32:44.36 Intake.select :open @21:32:46.52 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:32:46.97 SS.reconfigure :inhaling 0.41ml/s @21:32:47.03 SS.seek 25ml @21:33:57.64 Stroke completed -- waiting for pressure to equalize ... @21:33:57.65 SS.reconfigure :exhale @21:33:57.70 SS.hold @21:34:06.28 Intake.select :close @21:34:06.42 SSV.dial :exhaust @21:34:07.13 Exhaust.select :open @21:34:07.24 SS.seek 0ml @21:34:16.66 Exhaust.select :close @21:34:16.76 SSV.dial CBV @21:34:16.79
CBV.dial SSV @21:34:17.52 Allowing 130s for 25.0ml of remaining 425.0ml @21:34:17.53 Intake.select :open @21:34:19.68 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:34:19.70 SS.reconfigure :inhaling 0.41ml/s @21:34:19.74 SS.seek 25ml @21:35:15.23 Vacuum too high -- waiting up to 40 seconds @21:35:15.24 SS.reconfigure :exhale @21:35:15.28 SS.hold @21:35:20.80 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:35:20.82 SS.reconfigure :inhaling 0.37ml/s @21:35:20.86 SS.seek 25ml @21:35:38.66 Stroke completed -- waiting for pressure to equalize ... @21:35:38.67 SS.reconfigure :exhale @21:35:38.71 SS.hold @21:35:46.08 Intake.select :close @21:35:46.21 SSV.dial :exhaust @21:35:46.94 Exhaust.select :open @21:35:47.05 SS.seek 0ml @21:35:56.00 Exhaust.select :close @21:35:56.10 SSV.dial CBV @21:35:56.13
CBV.dial SSV @21:35:56.86 Allowing 130s for 25.0ml of remaining 400.0ml @21:35:56.87 Intake.select :open @21:35:59.02 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:35:59.04 SS.reconfigure :inhaling 0.37ml/s @21:35:59.08 SS.seek 25ml @21:37:19.51 Stroke completed -- waiting for pressure to equalize ... @21:37:19.52 SS.reconfigure :exhale @21:37:19.56 SS.hold @21:37:29.21 Intake.select :close @21:37:29.35 SSV.dial :exhaust @21:37:30.07 Exhaust.select :open @21:37:30.17 SS.seek 0ml @21:37:39.06 Exhaust.select :close @21:37:39.16 SSV.dial CBV @21:37:39.19
CBV.dial SSV @21:37:39.92 Allowing 130s for 25.0ml of remaining 375.0ml @21:37:39.93 Intake.select :open @21:37:42.08 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:37:42.10 SS.reconfigure :inhaling 0.37ml/s @21:37:42.14 SS.seek 25ml @21:38:53.10 Vacuum too high -- waiting up to 40 seconds @21:38:53.11 SS.reconfigure :exhale @21:38:53.15 SS.hold @21:38:58.45 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:38:58.47 SS.reconfigure :inhaling 0.33ml/s @21:38:58.52 SS.seek 25ml @21:39:10.29 Stroke completed -- waiting for pressure to equalize ... @21:39:10.30 SS.reconfigure :exhale @21:39:10.34 SS.hold @21:39:18.87 Intake.select :close @21:39:19.00 SSV.dial :exhaust @21:39:19.73 Exhaust.select :open @21:39:19.84 SS.seek 0ml @21:39:29.07 Exhaust.select :close @21:39:29.17 SSV.dial CBV @21:39:29.20
CBV.dial SSV @21:39:29.93 Allowing 130s for 25.0ml of remaining 350.0ml @21:39:29.94 Intake.select :open @21:39:32.09 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:39:32.11 SS.reconfigure :inhaling 0.33ml/s @21:39:32.15 SS.seek 25ml @21:41:04.47 Stroke completed -- waiting for pressure to equalize ... @21:41:04.48 SS.reconfigure :exhale @21:41:04.53 SS.hold @21:41:14.25 Intake.select :close @21:41:14.38 SSV.dial :exhaust @21:41:15.11 Exhaust.select :open @21:41:15.21 SS.seek 0ml @21:41:24.65 Exhaust.select :close @21:41:24.75 SSV.dial CBV @21:41:24.78
CBV.dial SSV @21:41:25.51 Allowing 130s for 25.0ml of remaining 325.0ml @21:41:25.52 Intake.select :open @21:41:27.67 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:41:27.68 SS.reconfigure :inhaling 0.33ml/s @21:41:27.73 SS.seek 25ml @21:42:54.99 Vacuum too high -- waiting up to 40 seconds @21:42:55.00 SS.reconfigure :exhale @21:42:55.05 SS.hold @21:42:59.77 Intake @13.8psia, vacuum from 8.3 to 11.3psi @21:42:59.79 SS.reconfigure :inhaling 0.30ml/s @21:42:59.83 SS.seek 25ml @21:43:05.69 Stroke completed -- waiting for pressure to equalize ... @21:43:05.70 SS.reconfigure :exhale @21:43:05.74 SS.hold @21:43:14.25 Intake.select :close @21:43:14.39 SSV.dial :exhaust @21:43:15.11 Exhaust.select :open @21:43:15.22 SS.seek 0ml @21:43:24.24 Exhaust.select :close @21:43:24.34 SSV.dial CBV @21:43:24.36
CBV.dial SSV @21:43:25.10 Allowing 130s for 25.0ml of remaining 300.0ml @21:43:25.11 Intake.select :open @21:43:27.26 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:43:27.27 SS.reconfigure :inhaling 0.30ml/s @21:43:27.32 SS.seek 25ml @21:45:07.30 Vacuum too high -- waiting up to 40 seconds @21:45:07.31 SS.reconfigure :exhale @21:45:07.35 SS.hold @21:45:14.16 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:45:14.18 SS.reconfigure :inhaling 0.27ml/s @21:45:14.22 SS.seek 25ml @21:45:15.53 Stroke completed -- waiting for pressure to equalize ... @21:45:15.54 SS.reconfigure :exhale @21:45:15.58 SS.hold @21:45:21.97 Intake.select :close @21:45:22.10 SSV.dial :exhaust @21:45:22.81 Exhaust.select :open @21:45:22.92 SS.seek 0ml @21:45:32.28 Exhaust.select :close @21:45:32.38 SSV.dial CBV @21:45:32.41
CBV.dial SSV @21:45:33.14 Allowing 130s for 25.0ml of remaining 275.0ml @21:45:33.15 Intake.select :open @21:45:35.31 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:45:35.32 SS.reconfigure :inhaling 0.27ml/s @21:45:35.37 SS.seek 25ml @21:47:25.29 Stroke completed -- waiting for pressure to equalize ... @21:47:25.30 SS.reconfigure :exhale @21:47:25.34 SS.hold @21:47:37.98 Intake.select :close @21:47:38.11 SSV.dial :exhaust @21:47:38.84 Exhaust.select :open @21:47:38.95 SS.seek 0ml @21:47:47.96 Exhaust.select :close @21:47:48.07 SSV.dial CBV @21:47:48.09
CBV.dial SSV @21:47:48.82 Allowing 130s for 25.0ml of remaining 250.0ml @21:47:48.83 Intake.select :open @21:47:50.99 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:47:51.00 SS.reconfigure :inhaling 0.27ml/s @21:47:51.05 SS.seek 25ml @21:49:42.55 Stroke completed -- waiting for pressure to equalize ... @21:49:42.56 SS.reconfigure :exhale @21:49:42.60 SS.hold @21:49:56.84 Intake.select :close @21:49:56.97 SSV.dial :exhaust @21:49:57.68 Exhaust.select :open @21:49:57.78 SS.seek 0ml @21:50:07.24 Exhaust.select :close @21:50:07.34 SSV.dial CBV @21:50:07.37
CBV.dial SSV @21:50:08.10 Allowing 130s for 25.0ml of remaining 224.9ml @21:50:08.11 Intake.select :open @21:50:10.26 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:50:10.28 SS.reconfigure :inhaling 0.27ml/s @21:50:10.32 SS.seek 25ml @21:51:40.60 Vacuum too high -- waiting up to 40 seconds @21:51:40.61 SS.reconfigure :exhale @21:51:40.65 SS.hold @21:51:49.56 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:51:49.57 SS.reconfigure :inhaling 0.24ml/s @21:51:49.62 SS.seek 25ml @21:52:12.45 Stroke completed -- waiting for pressure to equalize ... @21:52:12.46 SS.reconfigure :exhale @21:52:12.51 SS.hold @21:52:18.12 SS.reconfigure :default @21:52:18.16 SS.hold @21:52:18.26 Intake.select :close @21:52:18.40 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 800.1ml @21:52:18.42 SSV.dial :exhaust @21:52:19.14 Exhaust.select :open @21:52:19.25 SS.reconfigure :exhale @21:52:19.29 SS.seek 0ml @21:52:28.72 Exhaust.select :close @21:52:28.82 Sampled 800.1ml @21:52:28.83 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @21:52:28.84 Intake.select :close @21:52:28.90 CSV.dial :waste @21:52:28.93 CS.seek 0ml @21:52:29.06 SSV.dial CBV,avoiding: :exhaust @21:52:29.09 CSV.dial :air @21:52:29.12
CBV.dial SSV @21:52:29.84 CS.seek 10ml @21:52:30.93
CTV.dial :air @21:52:31.65 delay 15 seconds @21:52:46.67 SSV.dial :spit,avoiding: :exhaust @21:52:47.94 CTV.dial CSV,avoiding: :intake @21:52:47.98
SS.seek 0ml @21:52:48.09 SSV.dial CBV,avoiding: :exhaust @21:52:52.37 CBV.dial between SSV and waste2 @21:52:52.75 CTV.dial CSV,avoiding: :air @21:52:52.78
CSV.dial CTV @21:52:53.21 CS.reconfigure :pressurize @21:52:53.26 CS.seek 0ml @21:53:11.94 BubblePoint: Intake @40.2psia, Outlet @16.1psia @21:53:11.95 CBV.dial SSV @21:53:12.28 SS.reconfigure :default @21:53:12.32 SS.seek 10ml @21:53:17.76 delay 15 seconds @21:53:32.87 Evacuated: Intake @16.0psia, Outlet @11.1psia @21:53:32.88 SSV.dial :spit,avoiding: :exhaust @21:53:34.18 SS.reconfigure :exhale @21:53:34.22 SS.seek 0ml @21:53:39.66 SS.reconfigure :fast @21:53:39.70 SS.seek 5ml @21:53:42.75 SSV.dial CBV,avoiding: :exhaust @21:53:42.78
CBV.dial SSV @21:53:44.07 CTV.dial :air,avoiding: :intake @21:53:45.32 CSV.dial CTV @21:53:45.35
SS.seek 10ml @21:53:46.35 CSV.dial :air @21:53:46.79 CS.reconfigure :fast @21:53:46.83 CS.seek 0ml @21:53:48.05
SSV.dial :spit,avoiding: :exhaust @21:53:49.33 SS.reconfigure :exhale @21:53:49.38 SS.seek 0ml @21:53:53.72 CS.stop @21:53:54.93
SS.reconfigure :fast @21:53:54.97 SS.seek 5ml @21:53:57.58 SSV.dial CBV,avoiding: :exhaust @21:53:57.61
CBV.dial SSV @21:53:59.90 SSV.dial :spit,avoiding: :exhaust @21:54:01.19 SS.reconfigure :default @21:54:01.23 SS.seek 0ml @21:54:04.55 SS.stop @21:54:04.59 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @21:54:04.66 CSV.dial :waste @21:54:04.77 SSV.dial CBV,avoiding: :exhaust @21:54:04.79
CBV.dial SSV @21:54:05.38 CS.seek 0ml @21:54:05.45 CSV.dial :air @21:54:06.08
CTV.dial :air @21:54:06.09 delay 15 seconds @21:54:06.17 CS.seek 10ml @21:54:21.11
SSV.dial :spit,avoiding: :exhaust @21:54:22.39 CTV.dial CSV,avoiding: :intake @21:54:22.43
SS.reconfigure :exhale @21:54:22.97 SS.seek 0ml @21:54:23.03 SSV.dial CBV,avoiding: :exhaust @21:54:28.71 CBV.dial between SSV and waste2 @21:54:29.10 CTV.dial CSV,avoiding: :air @21:54:29.13
CSV.dial CTV @21:54:29.68 CS.reconfigure :pressurize @21:54:29.73 CS.seek 0ml @21:54:50.82 BubblePoint: Intake @39.5psia, Outlet @15.4psia CBV.dial SSV @21:54:51.15 SS.reconfigure :default @21:54:51.19 SS.seek 10ml @21:54:56.73 delay 15 seconds @21:55:11.85 Evacuated: Intake @18.2psia, Outlet @12.4psia @21:55:11.86 SSV.dial :spit,avoiding: :exhaust @21:55:13.14 SS.reconfigure :exhale @21:55:13.19 SS.seek 0ml @21:55:18.65 SS.reconfigure :fast @21:55:18.69 SS.seek 5ml @21:55:21.50 SSV.dial CBV,avoiding: :exhaust @21:55:21.53
CBV.dial SSV @21:55:22.81 CTV.dial :air,avoiding: :intake @21:55:24.07 CSV.dial CTV @21:55:24.10
SS.seek 10ml @21:55:25.10 CSV.dial :air @21:55:25.52 CS.reconfigure :fast @21:55:25.57 CS.seek 0ml @21:55:26.59
SSV.dial :spit,avoiding: :exhaust @21:55:27.88 SS.reconfigure :exhale @21:55:27.92 SS.seek 0ml @21:55:30.47 CS.stop @21:55:33.54
SS.reconfigure :fast @21:55:33.58 SS.seek 5ml @21:55:36.19 SSV.dial CBV,avoiding: :exhaust @21:55:36.21
CBV.dial SSV @21:55:38.51 SSV.dial :spit,avoiding: :exhaust @21:55:39.78 SS.reconfigure :default @21:55:39.82 SS.seek 0ml @21:55:43.13 SS.stop @21:55:43.17 Lysing: Adding 2.0ml AqMeOH @21:55:43.18 CBV.dial between CSV and waste1,avoiding: :DBV @21:55:44.60 CTV.dial CSV @21:55:45.84 CSV.dial CSR @21:55:46.60 Pulling 1ml of CSR.AqMeOH @21:55:46.61 CSR.select :AqMeOH @21:55:46.72 CS.reconfigure :default @21:55:46.76 CS.seek 1ml @21:55:50.13 delay 5 seconds @21:55:55.14 CSR.select :air @21:55:55.30 CS.seek 6ml @21:56:08.78 delay 2 seconds @21:56:10.80 CSV.dial :waste2 @21:56:11.35 CS.reconfigure :fast @21:56:11.39 CS.seek 0ml @21:56:25.09 CSV.dial CSR @21:56:25.55 Pulling 2ml of CSR.AqMeOH @21:56:25.56 CSR.select :AqMeOH @21:56:25.67 CS.reconfigure :default @21:56:25.71 CS.seek 2ml @21:56:31.66 delay 5 seconds @21:56:36.67 CSR.select :air @21:56:36.83 CS.seek 5.001ml @21:56:45.19 delay 2 seconds @21:56:47.20 CSV.dial CTV @21:56:47.61 CS.seek 1ml @21:56:58.60 Before lysing Collection Puck Heater at 20.8C/39.1psia @21:56:58.63 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @21:56:58.64 CH.seek 70C,8:00 @21:58:39.61 CH.hold 10:00,70C @21:58:39.78 Begun lysing Collection Puck Heater at 68C/42.7psia @22:08:39.79 Ended lysing Collection Puck Heater at 70C/42.3psia @22:08:39.80 CS.seek 0ml @22:08:43.14 CTV.dial between spare and air,avoiding: :intake @22:08:44.12 CSV.dial :air @22:08:44.56 CS.seek 5ml @22:08:57.95 CSV.dial CTV @22:08:58.37 delay 2 seconds @22:09:00.39 CSV.dial :air @22:09:00.82 delay 2 seconds @22:09:02.84 CSV.dial CTV @22:09:03.28 delay 2 seconds @22:09:05.30 CSV.dial :air @22:09:05.73 delay 2 seconds @22:09:07.75 CS.seek 0ml @22:09:21.28 Puck @67.6psia after final lysis pressurization @22:09:21.29 Recovering Lysate into Collection syringe @22:09:21.30 CSV.dial CBV @22:09:22.28 CBV.dial CSV @22:09:22.63 CS.seek 5ml @22:09:36.07 delay 5 seconds @22:09:41.09 CTV.dial :air @22:09:41.58 CS.seek 7ml @22:09:47.45 delay 5 seconds @22:09:52.46 CSV.dial :air2 @22:09:52.91 CS.seek 10ml @22:10:01.29 CSV.dial between air and CTV,avoiding: CBV @22:10:02.41 Lysing Complete @22:10:02.47 Storing sh1 puck in Collection Clamp to tube 1 @22:10:02.63 SC.reconfigure :tight @22:10:02.67 SC.seek 1 @22:10:06.30 SC.configure :limp @22:10:06.49 Elbow.reconfigure :tight @22:10:06.53 Elbow.seek 1 @22:10:08.40 Forearm.reconfigure :tight @22:10:08.44 Forearm.seek 1 + 700 counts @22:10:09.48 SE.reconfigure :pick @22:10:09.53 SE.seek :beamBreak @22:10:11.89 SE.configure :default @22:10:12.03 Elbow.reconfigure :limp @22:10:12.07 Forearm.reconfigure :loose @22:10:12.11 Forearm.seek 1 @22:10:12.49 Elbow.hold @22:10:12.55 Elbow.reconfigure :tight @22:10:12.64 SE.seek 11.28 Pucks below Top Plate @22:10:13.21 Forearm.force -20 @22:10:13.24 Forearm.seek 1 + 305 counts @22:10:13.55 Forearm.reconfigure :tight @22:10:13.59 Forearm.seek 1 + 700 counts @22:10:13.94 SE.seek 12.28 Pucks below Top Plate @22:10:14.52 Forearm.reconfigure :loose @22:10:14.56 Forearm.seek :retract @22:10:15.51 Elbow.seek CC @22:10:17.22 Elbow.reconfigure :loose @22:10:17.32 Forearm.seek CC @22:10:18.24 Hand.close @22:10:18.82 CC.seek :open @22:10:23.25 Forearm.seek :retract @22:10:24.22 Elbow.reconfigure :tight @22:10:24.26 Elbow.seek 1 @22:10:25.93 Elbow.reconfigure :limp @22:10:25.97 Forearm.seek 1 @22:10:27.12 Elbow.hold @22:10:27.18 Elbow.reconfigure :tight @22:10:27.22 Hand.open @22:10:28.57 Forearm.force -20 @22:10:28.61 Forearm.seek 1 + 317 counts @22:10:28.92 Forearm.reconfigure :tight @22:10:28.96 Forearm.seek 1 + 700 counts @22:10:29.40 SE.seek :down @22:10:30.52
Forearm.reconfigure :loose @22:10:30.56 Forearm.seek :retract @22:10:31.37 SE.stop @22:10:31.59
Tranferring DA @22:10:31.60 Beginning samplePassC @22:10:31.61 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:10:31.80 Elbow.seek FlushPuck::Garage @22:10:33.77 Elbow.reconfigure :loose @22:10:33.86 Forearm.seek :garage @22:10:34.96 Hand.close @22:10:35.68 Forearm.seek :retract @22:10:36.71 Elbow.reconfigure :tight @22:10:36.76 Elbow.seek CC @22:10:39.52 Elbow.reconfigure :loose @22:10:39.60 Forearm.reconfigure :tight @22:10:39.65 Forearm.seek CC @22:10:40.87 CC.seek :closed @22:10:45.10 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.94 @22:10:45.17 Hand.open @22:10:46.00 Forearm.reconfigure :loose @22:10:46.04 Forearm.seek :retract @22:10:47.12 PSV.dial PSR @22:10:48.41 Pulling 1.5ml of PSR.flush PSR.select :flush @22:10:48.53 PS.seek 1.5ml @22:11:04.50 delay 5 seconds @22:11:09.52 PSV.dial :waste1 @22:11:09.95 PS.seek 0ml @22:11:26.21 PSV.dial PSR @22:11:26.65 PSR.select :air @22:11:26.76 PS.seek 2.5ml @22:11:52.87 PRV.dial CBV,avoiding: :ab2 @22:11:52.91
CBV.dial PRV @22:11:54.51 PSV.dial :mixloop @22:11:55.48 CTV.dial :air,avoiding: :intake @22:11:55.49 PTV.dial :PRVmixing @22:11:56.72 PS.seek 0ml @22:12:22.83 PSV.dial :air @22:12:23.26 PS.seek 2.5ml @22:12:49.40 PSV.dial :mixloop @22:12:49.84 PS.seek 0ml @22:13:16.08 PSV.dial :air @22:13:16.53 PS.seek 2.5ml @22:13:42.77 PSV.dial :mixloop @22:13:43.21 PS.seek 0ml @22:14:09.33 Garaging FlushPuck in Collection Clamp @22:14:09.40 Elbow.reconfigure :tight @22:14:09.44 Elbow.seek CC @22:14:09.55 Elbow.reconfigure :loose @22:14:09.64 Forearm.seek CC @22:14:10.58 Hand.close @22:14:11.19 CC.seek :open @22:14:15.52 Forearm.seek :retract @22:14:16.58 Elbow.reconfigure :tight @22:14:16.63 Elbow.seek :garage @22:14:19.73 Elbow.reconfigure :loose @22:14:19.81 Forearm.reconfigure :tight @22:14:19.85 Forearm.seek :garage @22:14:20.94 Hand.open @22:14:21.75 Forearm.reconfigure :loose @22:14:22.26 Forearm.seek :retract @22:14:23.34 PSV.dial :mixloop @22:14:23.36 PTV.dial :PRVmixing @22:14:23.37 PRV.dial CBV @22:14:23.39
CBV.dial PRV @22:14:23.41 CTV.dial :air,avoiding: :intake @22:14:23.43 Loading lyfil puck from tube 2 into Collection Clamp @22:14:23.64 SC.reconfigure :tight @22:14:23.69 SC.seek 2 @22:14:27.30 SC.configure :limp @22:14:27.50 Elbow.reconfigure :tight @22:14:27.54 Elbow.seek 2 @22:14:29.41 Forearm.reconfigure :tight @22:14:29.45 Forearm.seek 2 + 700 counts @22:14:30.47 SE.reconfigure :pick @22:14:30.52 SE.seek :beamBreak @22:14:32.33 SE.configure :default @22:14:32.47 Elbow.seek 2 @22:14:32.64 Forearm.seek 2 + 300 counts @22:14:32.98 Forearm.reconfigure :loose @22:14:33.03 Hand.close @22:14:33.70 SE.seek :down @22:14:33.89
Forearm.seek :retract @22:14:34.85 Elbow.seek CC @22:14:35.25 SE.stop @22:14:36.42
Elbow.reconfigure :loose @22:14:36.50 Forearm.reconfigure :tight @22:14:36.55 Forearm.seek CC @22:14:37.87 CC.seek :closed @22:14:42.08 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.68 @22:14:42.09 Hand.open @22:14:42.87 Forearm.reconfigure :loose @22:14:42.91 Forearm.seek :retract @22:14:43.91 CTV.dial CSV,avoiding: :intake @22:14:45.16 CSV.dial CTV @22:14:45.48 PS.seek 2.5ml @22:15:11.80 CS.seek 5.5ml @22:15:24.00 delay 8 seconds @22:15:32.02 PSV.dial :air @22:15:32.47 delay 2 seconds @22:15:34.49 PSV.dial :waste2 @22:15:35.18 PS.seek 0ml @22:16:01.39 CBV.dial between PRV and waste1 @22:16:01.71 CSV.dial :air @22:16:02.15 CS.seek 10ml @22:16:14.28 CSV.dial CTV @22:16:14.82 CS.seek 0ml @22:16:40.84 CBV.dial :waste1 @22:16:41.14 delay 5 seconds @22:16:46.16 CTV.dial :air,avoiding: :intake @22:16:47.44 Storing lyfil puck in Collection Clamp to tube 1 @22:16:47.60 SC.reconfigure :tight @22:16:47.64 SC.seek 1 @22:16:51.27 SC.configure :limp @22:16:51.47 Elbow.reconfigure :tight @22:16:51.51 Elbow.seek 1 @22:16:53.26 Forearm.reconfigure :tight @22:16:53.31 Forearm.seek 1 + 700 counts @22:16:54.46 SE.reconfigure :pick @22:16:54.51 SE.seek :beamBreak @22:16:56.79 SE.configure :default @22:16:56.93 Elbow.reconfigure :limp @22:16:56.97 Forearm.reconfigure :loose @22:16:57.48 Forearm.seek 1 @22:16:57.90 Elbow.hold @22:16:57.96 Elbow.reconfigure :tight @22:16:58.06 SE.seek 12.29 Pucks below Top Plate @22:16:58.49 Forearm.force -20 @22:16:58.53 Forearm.seek 1 + 313 counts @22:16:58.82 Forearm.reconfigure :tight @22:16:58.86 Forearm.seek 1 + 700 counts @22:16:59.14 SE.seek 13.29 Pucks below Top Plate @22:16:59.70 Forearm.reconfigure :loose @22:16:59.75 Forearm.seek :retract @22:17:00.67 Elbow.seek CC @22:17:02.34 Elbow.reconfigure :loose @22:17:02.43 Forearm.seek CC @22:17:03.42 Hand.close @22:17:04.19 CC.seek :open @22:17:08.61 Forearm.seek :retract @22:17:09.58 Elbow.reconfigure :tight @22:17:09.62 Elbow.seek 1 @22:17:11.40 Elbow.reconfigure :limp @22:17:11.45 Forearm.seek 1 @22:17:12.57 Elbow.hold @22:17:12.63 Elbow.reconfigure :tight @22:17:12.68 Hand.open @22:17:13.55 Forearm.force -20 @22:17:13.58 Forearm.seek 1 + 314 counts @22:17:13.95 Forearm.reconfigure :tight @22:17:13.99 Forearm.seek 1 + 700 counts @22:17:14.26 SE.seek :down @22:17:15.39
Forearm.reconfigure :loose @22:17:15.43 Forearm.seek :retract @22:17:16.17 SE.stop @22:17:16.39
PSV.dial :air @22:17:17.08 PS.seek 2.5ml @22:17:43.33 delay 1 second @22:17:44.34 PSV.dial :mixloop @22:17:44.77 delay 1 second @22:17:45.78 PSV.dial :air @22:17:46.22 delay 1 second @22:17:47.23 PSV.dial :mixloop @22:17:47.66 delay 1 second @22:17:48.67 PSV.dial :air @22:17:49.11 delay 1 second @22:17:50.13 PS.seek 0ml @22:18:16.45 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:18:16.62 Elbow.seek FlushPuck::Garage @22:18:18.61 Elbow.reconfigure :loose @22:18:18.70 Forearm.seek :garage @22:18:19.81 Hand.close @22:18:20.54 Forearm.seek :retract @22:18:21.66 Elbow.reconfigure :tight @22:18:21.70 Elbow.seek PC @22:18:23.05 Elbow.reconfigure :loose @22:18:23.14 Forearm.reconfigure :tight @22:18:23.18 Forearm.seek PC @22:18:24.50 PC.seek :closed @22:18:28.40 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.43 @22:18:28.47 Hand.open @22:18:29.24 Forearm.reconfigure :loose @22:18:29.28 Forearm.seek :retract @22:18:30.32 PRV.dial :air @22:18:30.76 PSV.dial :mixloop @22:18:31.85 PS.seek 0.3ml @22:18:35.67 PSV.dial PSR @22:18:36.71 Pulling 0.4ml of PSR.air PSR.select :air @22:18:36.74 PS.seek 0.7ml @22:18:41.57 delay 3 seconds @22:18:44.59 PSR.select :air @22:18:44.61 PS.seek 2.5ml @22:19:03.70 delay 3 seconds @22:19:06.72 PSV.dial :mixloop @22:19:07.70 PTV.dial :mixing @22:19:08.40 PBV.dial :waste2 @22:19:09.27 PS.seek 0ml @22:19:35.42 Garaging FlushPuck in Processing Clamp @22:19:35.49 Elbow.reconfigure :tight @22:19:35.53 Elbow.seek PC @22:19:35.64 Elbow.reconfigure :loose @22:19:35.73 Forearm.seek PC @22:19:36.85 Hand.close @22:19:37.43 PC.seek :open @22:19:41.37 Forearm.seek :retract @22:19:42.42 Elbow.reconfigure :tight @22:19:42.46 Elbow.seek :garage @22:19:43.83 Elbow.reconfigure :loose @22:19:43.91 Forearm.reconfigure :tight @22:19:43.95 Forearm.seek :garage @22:19:45.02 Hand.open @22:19:45.86 Forearm.reconfigure :loose @22:19:45.91 Forearm.seek :retract @22:19:46.96 Loading da puck from tube 2 into Processing Clamp @22:19:47.18 SC.reconfigure :tight @22:19:47.22 SC.seek 2 @22:19:50.84 SC.configure :limp @22:19:51.03 Elbow.reconfigure :tight @22:19:51.07 Elbow.seek 2 @22:19:53.10 Forearm.reconfigure :tight @22:19:53.14 Forearm.seek 2 + 700 counts @22:19:54.20 SE.reconfigure :pick @22:19:54.25 SE.seek :beamBreak @22:19:56.14 SE.configure :default @22:19:56.28 Elbow.seek 2 @22:19:56.66 Forearm.seek 2 + 300 counts @22:19:56.93 Forearm.reconfigure :loose @22:19:56.97 Hand.close @22:19:57.57 SE.seek :down @22:19:57.75
Forearm.seek :retract @22:19:58.78 Elbow.seek PC @22:19:59.26 SE.stop @22:20:01.23
Elbow.reconfigure :loose @22:20:01.32 Forearm.reconfigure :tight @22:20:01.37 Forearm.seek PC @22:20:02.58 PC.seek :closed @22:20:06.45 Processing Clamp onSpring/offSpring for 8/23 samples, on/off torque 1.40 @22:20:06.46 Hand.open @22:20:07.24 Forearm.reconfigure :loose @22:20:07.28 Forearm.seek :retract @22:20:08.30 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @22:20:08.31 PH.seek 28C,5:00 @22:20:52.80 PH.hold 2:00:00,28C @22:20:52.84 delay 1:30 @22:22:22.89 PSR.select :air @22:22:22.91 PSV.dial PSR @22:22:23.94 Pulling 0.5ml of PSR.wash @22:22:23.95 PSR.select :wash @22:22:24.06 PS.seek 0.5ml @22:22:29.95 delay 5 seconds @22:22:34.96 PSR.select :air @22:22:35.08 PS.seek 2ml @22:22:51.13 PTV.dial :puck @22:22:51.82 PSV.dial PTV @22:22:52.65 PBV.dial :waste1 @22:22:53.94 PS.seek 1.5ml @22:22:59.91 delay 3 seconds @22:23:02.93 PBV.dial between PSV and waste1 @22:23:03.30 PS.seek 0ml @22:23:19.35 delay 5:00 @22:28:19.37 recoverPSRreagent08 @22:28:19.38 PTV.dial :puck @22:28:19.39 PBV.dial :waste1 @22:28:19.75 delay 30 seconds @22:28:49.77 PBV.dial between PSV and waste1 @22:28:50.12 PSR.select :air @22:28:50.14 PSV.dial PSR @22:28:50.95 PS.reconfigure :fast @22:28:51.00 PS.seek 2.5ml @22:29:13.59 PSV.dial PTV @22:29:14.26 delay 5 seconds @22:29:19.28 PSV.dial PSR @22:29:19.98 delay 5 seconds @22:29:25.00 PSV.dial PTV @22:29:25.70 delay 5 seconds @22:29:30.72 PSV.dial :air @22:29:31.42 PS.seek 0ml @22:29:54.03 PTV.dial :PRVmixing,via: PRV @22:29:55.29 PSV.dial :mixloop @22:29:55.95 PRV.dial :ab1 @22:29:56.67 PS.reconfigure :slow @22:29:56.71 PS.seek 0.15ml @22:30:00.24 delay 2 seconds @22:30:02.26 CBV.dial PRV @22:30:02.30
PRV.dial CBV @22:30:02.84 PS.seek 0.34ml @22:30:07.18 delay 2 seconds @22:30:09.20 CBV.dial between PRV and PSV @22:30:09.49 PRV.dial :air @22:30:09.94 PS.seek 0.9ml @22:30:21.74 PRV.dial :waste2 @22:30:22.44 PS.seek 0.5ml @22:30:31.12 addPMLReagent08 @22:30:31.13 PSV.dial :air @22:30:31.58 PS.reconfigure :default @22:30:31.62 PS.seek 2ml @22:30:47.65 PSV.dial :PML @22:30:48.09 PTV.dial :mixing @22:30:48.82 PBV.dial :waste1 @22:30:49.24 PS.seek 1.85ml @22:30:51.65 PBV.dial between PSV and waste1 @22:30:52.03 PS.seek 0ml @22:31:11.62 delay 2 seconds @22:31:13.63 Beginning samplePassC @22:31:13.65 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:31:13.80 Elbow.reconfigure :tight @22:31:13.85 Elbow.seek FlushPuck::Garage @22:31:15.38 Elbow.reconfigure :loose @22:31:15.47 Forearm.seek :garage @22:31:16.56 Hand.close @22:31:17.27 Forearm.seek :retract @22:31:18.38 Elbow.reconfigure :tight @22:31:18.42 Elbow.seek CC @22:31:21.32 Elbow.reconfigure :loose @22:31:21.41 Forearm.reconfigure :tight @22:31:21.45 Forearm.seek CC @22:31:22.66 CC.seek :closed @22:31:26.89 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.81 @22:31:26.97 Hand.open @22:31:28.18 Forearm.reconfigure :loose @22:31:28.23 Forearm.seek :retract @22:31:29.34 PSV.dial PSR @22:31:30.36 Pulling 1.5ml of PSR.flush @22:31:30.37 PSR.select :flush @22:31:30.48 PS.seek 1.5ml @22:31:46.59 delay 5 seconds @22:31:51.61 PSV.dial :waste1 @22:31:52.05 PS.seek 0ml @22:32:08.18 PSV.dial PSR @22:32:08.61 PSR.select :air @22:32:08.72 PS.seek 2.5ml @22:32:35.09 PRV.dial CBV,avoiding: :ab2 @22:32:35.11
CBV.dial PRV @22:32:36.68 PSV.dial :mixloop @22:32:37.66 CTV.dial :air,avoiding: :intake @22:32:37.67 PTV.dial :PRVmixing @22:32:38.39 PS.seek 0ml @22:33:04.52 PSV.dial :air @22:33:04.95 PS.seek 2.5ml @22:33:31.08 PSV.dial :mixloop @22:33:31.52 PS.seek 0ml @22:33:57.70 PSV.dial :air @22:33:58.15 PS.seek 2.5ml @22:34:24.48 PSV.dial :mixloop @22:34:24.93 PS.seek 0ml @22:34:51.11 Garaging FlushPuck in Collection Clamp @22:34:51.18 Elbow.reconfigure :tight @22:34:51.22 Elbow.seek CC @22:34:51.33 Elbow.reconfigure :loose @22:34:51.43 Forearm.seek CC @22:34:52.40 Hand.close @22:34:53.15 CC.seek :open @22:34:57.48 Forearm.seek :retract @22:34:58.45 Elbow.reconfigure :tight @22:34:58.49 Elbow.seek :garage @22:35:01.37 Elbow.reconfigure :loose @22:35:01.46 Forearm.reconfigure :tight @22:35:01.51 Forearm.seek :garage @22:35:02.61 Hand.open @22:35:03.46 Forearm.reconfigure :loose @22:35:03.50 Forearm.seek :retract @22:35:04.59 delayUntil "22:39:13.62" @22:35:04.61 Whole Cell Archive sampling 1000ml @22:35:04.63 Loading wcr puck from tube 2 into Collection Clamp @22:35:04.85 SC.reconfigure :tight @22:35:04.90 SC.seek 2 @22:35:04.99 SC.configure :limp @22:35:05.19 Elbow.reconfigure :tight @22:35:05.24 Elbow.seek 2 @22:35:07.23 Forearm.reconfigure :tight @22:35:07.28 Forearm.seek 2 + 700 counts @22:35:08.35 SE.reconfigure :pick @22:35:08.41 SE.seek :beamBreak @22:35:10.32 SE.configure :default @22:35:10.47 Elbow.seek 2 @22:35:10.81 Forearm.seek 2 + 300 counts @22:35:11.24 Forearm.reconfigure :loose @22:35:11.28 Hand.close @22:35:11.91 SE.seek :down @22:35:12.11 Forearm.seek :retract @22:35:13.09 Elbow.seek CC @22:35:13.60 SE.stop @22:35:14.74 Elbow.reconfigure :loose @22:35:14.83 Forearm.reconfigure :tight @22:35:14.87 Forearm.seek CC @22:35:16.66 CC.seek :closed @22:35:20.97 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.68 @22:35:20.99 Hand.open @22:35:21.83 Forearm.reconfigure :loose @22:35:21.88 Forearm.seek :retract @22:35:22.98 collectSample 1000ml w/bubble point of 35psi @22:35:22.99 Priming 7ml @22:35:23.00 Intake.select :close @22:35:23.01 Exhaust.select :close @22:35:23.02 SS.hold @22:35:23.21 Calibrating pressure sensors @22:35:23.22 CTV.dial :air @22:35:23.23 SSV.dial :spit,avoiding: :exhaust @22:35:23.25 SS.reconfigure :fast @22:35:23.30 SS.seek 5ml @22:35:26.05 SSV.dial CBV @22:35:26.07 CBV.dial SSV @22:35:27.36 delay 3.8 seconds @22:35:31.29 SSV.dial :spit @22:35:32.57 SS.seek 0ml @22:35:35.62 SSV.dial CBV @22:35:36.90 CTV.dial :intake @22:35:37.63 SSV.dial CBV @22:35:37.65 CBV.dial SSV @22:35:37.67 Allowing 40s for 7.0ml of remaining 7.0ml @22:35:37.68 Intake.select :open @22:35:39.83 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:35:39.85 SS.reconfigure :inhaling 2.14ml/s @22:35:39.89 SS.seek 7ml @22:35:45.85 Stroke completed -- waiting for pressure to equalize ... @22:35:45.87 SS.reconfigure :exhale @22:35:45.91 SS.hold @22:35:50.08 Intake.select :close @22:35:50.22 SSV.dial :exhaust @22:35:50.95 Exhaust.select :open @22:35:51.07 SS.seek 0ml @22:35:55.38 Exhaust.select :close @22:35:55.49 Sampling 1000.0ml @22:35:55.50 Intake.select :close @22:35:55.51 Exhaust.select :close @22:35:55.53 SS.reconfigure :default @22:35:55.56 SS.hold @22:35:55.76 CTV.dial :intake @22:35:55.78 SSV.dial CBV @22:35:55.80 CBV.dial SSV @22:35:56.54 Allowing 130s for 25.0ml of remaining 1000.0ml @22:35:56.56 Intake.select :open @22:35:58.71 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:35:58.73 SS.reconfigure :inhaling 2.14ml/s @22:35:58.77 SS.seek 25ml @22:36:03.67 Vacuum too high -- waiting up to 40 seconds @22:36:03.69 SS.reconfigure :exhale @22:36:03.73 SS.hold @22:36:05.63 Intake @13.1psia, vacuum from 7.6 to 10.6psi @22:36:05.65 SS.reconfigure :inhaling 1.93ml/s @22:36:05.70 SS.seek 25ml @22:36:08.46 Vacuum too high -- waiting up to 40 seconds @22:36:08.47 SS.reconfigure :exhale @22:36:08.51 SS.hold @22:36:10.40 Intake @13.6psia, vacuum from 8.1 to 11.1psi @22:36:10.42 SS.reconfigure :inhaling 1.74ml/s @22:36:10.46 SS.seek 25ml @22:36:13.14 Vacuum too high -- waiting up to 40 seconds @22:36:13.16 SS.reconfigure :exhale @22:36:13.20 SS.hold @22:36:14.73 Intake @13.5psia, vacuum from 8.0 to 11.0psi @22:36:14.75 SS.reconfigure :inhaling 1.57ml/s @22:36:15.27 SS.seek 25ml @22:36:19.24 Vacuum too high -- waiting up to 40 seconds @22:36:19.25 SS.reconfigure :exhale @22:36:19.30 SS.hold @22:36:20.75 Intake @13.1psia, vacuum from 7.6 to 10.6psi @22:36:20.76 SS.reconfigure :inhaling 1.41ml/s @22:36:20.81 SS.seek 25ml @22:36:25.14 Vacuum too high -- waiting up to 40 seconds @22:36:25.15 SS.reconfigure :exhale @22:36:25.20 SS.hold @22:36:26.64 Intake @13.1psia, vacuum from 7.6 to 10.6psi @22:36:26.66 SS.reconfigure :inhaling 1.27ml/s @22:36:26.70 SS.seek 25ml @22:36:30.37 Stroke completed -- waiting for pressure to equalize ... @22:36:30.39 SS.reconfigure :exhale @22:36:30.43 SS.hold @22:36:31.97 Intake.select :close @22:36:32.12 SSV.dial :exhaust @22:36:32.85 Exhaust.select :open @22:36:32.97 SS.seek 0ml @22:36:42.34 Exhaust.select :close @22:36:42.44 SSV.dial CBV @22:36:42.47 CBV.dial SSV @22:36:43.21 Allowing 130s for 25.0ml of remaining 975.0ml @22:36:43.23 Intake.select :open @22:36:45.38 Intake @14.9psia, vacuum from 9.4 to 12.4psi @22:36:45.40 SS.reconfigure :inhaling 1.27ml/s @22:36:45.44 SS.seek 25ml @22:36:50.58 Vacuum too high -- waiting up to 40 seconds @22:36:50.59 SS.reconfigure :exhale @22:36:50.63 SS.hold @22:36:51.34 Intake @13.0psia, vacuum from 7.5 to 10.5psi @22:36:51.36 SS.reconfigure :inhaling 1.14ml/s @22:36:51.40 SS.seek 25ml @22:36:56.83 Vacuum too high -- waiting up to 40 seconds @22:36:56.84 SS.reconfigure :exhale @22:36:56.89 SS.hold @22:36:58.20 Intake @13.5psia, vacuum from 8.0 to 11.0psi @22:36:58.22 SS.reconfigure :inhaling 1.03ml/s @22:36:58.26 SS.seek 25ml @22:37:12.42 Vacuum too high -- waiting up to 40 seconds @22:37:12.43 SS.reconfigure :exhale @22:37:12.48 SS.hold @22:37:14.12 Intake @14.1psia, vacuum from 8.6 to 11.6psi @22:37:14.14 SS.reconfigure :inhaling 0.93ml/s @22:37:14.19 SS.seek 25ml @22:37:16.79 Stroke completed -- waiting for pressure to equalize ... @22:37:16.80 SS.reconfigure :exhale @22:37:16.84 SS.hold @22:37:17.41 Intake.select :close @22:37:17.55 SSV.dial :exhaust @22:37:18.27 Exhaust.select :open @22:37:18.38 SS.seek 0ml @22:37:27.37 Exhaust.select :close @22:37:27.47 SSV.dial CBV @22:37:27.50 CBV.dial SSV @22:37:28.24 Allowing 130s for 25.0ml of remaining 950.0ml @22:37:28.26 Intake.select :open @22:37:30.41 Intake @14.9psia, vacuum from 9.4 to 12.4psi @22:37:30.43 SS.reconfigure :inhaling 0.93ml/s @22:37:30.48 SS.seek 25ml @22:38:00.05 Stroke completed -- waiting for pressure to equalize ... @22:38:00.06 SS.reconfigure :exhale @22:38:00.11 SS.hold @22:38:02.60 Intake.select :close @22:38:02.75 SSV.dial :exhaust @22:38:03.48 Exhaust.select :open @22:38:03.59 SS.seek 0ml @22:38:12.46 Exhaust.select :close @22:38:13.05 SSV.dial CBV @22:38:13.08 CBV.dial SSV @22:38:13.82 Allowing 130s for 25.0ml of remaining 925.0ml @22:38:13.83 Intake.select :open @22:38:15.99 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:38:16.01 SS.reconfigure :inhaling 0.93ml/s @22:38:16.05 SS.seek 25ml @22:38:45.75 Stroke completed -- waiting for pressure to equalize ... @22:38:45.77 SS.reconfigure :exhale @22:38:45.81 SS.hold @22:38:48.71 Intake.select :close @22:38:48.85 SSV.dial :exhaust @22:38:49.58 Exhaust.select :open @22:38:49.70 SS.seek 0ml @22:38:58.83 Exhaust.select :close @22:38:58.94 SSV.dial CBV @22:38:58.98 CBV.dial SSV @22:38:59.69 Allowing 130s for 25.0ml of remaining 900.0ml @22:38:59.71 Intake.select :open @22:39:01.87 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:39:01.88 SS.reconfigure :inhaling 0.93ml/s @22:39:01.93 SS.seek 25ml @22:39:13.64 recoverPRVreagent08 @22:39:13.65 PTV.dial :mixing @22:39:14.37 PBV.dial :waste1 @22:39:14.71 delay 30 seconds @22:39:18.48 Vacuum too high -- waiting up to 40 seconds @22:39:18.49 SS.reconfigure :exhale @22:39:18.53 SS.hold @22:39:19.49 Intake @13.5psia, vacuum from 8.0 to 11.0psi @22:39:19.51 SS.reconfigure :inhaling 0.84ml/s @22:39:19.56 SS.seek 25ml @22:39:33.24 Stroke completed -- waiting for pressure to equalize ... @22:39:33.25 SS.reconfigure :exhale @22:39:33.29 SS.hold @22:39:35.24 Intake.select :close @22:39:35.38 SSV.dial :exhaust @22:39:36.11 Exhaust.select :open @22:39:36.22 SS.seek 0ml @22:39:44.73 PBV.dial between PSV and waste1 @22:39:45.09 PSV.dial :air @22:39:45.54 PS.reconfigure :fast @22:39:45.59 PS.seek 2.5ml @22:39:45.86 Exhaust.select :close @22:39:45.97 SSV.dial CBV @22:39:46.01 CBV.dial SSV @22:39:46.74 Allowing 130s for 25.0ml of remaining 875.0ml @22:39:46.75 Intake.select :open @22:39:48.90 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:39:48.92 SS.reconfigure :inhaling 0.84ml/s @22:39:48.96 SS.seek 25ml @22:40:08.26 PSV.dial :mixloop @22:40:08.71 delay 2 seconds @22:40:10.73 PSV.dial :air @22:40:11.18 delay 2 seconds @22:40:13.20 PSV.dial :mixloop @22:40:13.64 delay 2 seconds @22:40:15.66 PSV.dial :air @22:40:16.11 PS.seek 0ml @22:40:21.05 Stroke completed -- waiting for pressure to equalize ... @22:40:21.06 SS.reconfigure :exhale @22:40:21.10 SS.hold @22:40:24.00 Intake.select :close @22:40:24.14 SSV.dial :exhaust @22:40:24.86 Exhaust.select :open @22:40:24.97 SS.seek 0ml @22:40:34.36 Exhaust.select :close @22:40:34.47 SSV.dial CBV @22:40:34.50 CBV.dial SSV @22:40:35.22 Allowing 130s for 25.0ml of remaining 850.0ml @22:40:35.24 Intake.select :open @22:40:37.39 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:40:37.41 SS.reconfigure :inhaling 0.84ml/s @22:40:37.45 SS.seek 25ml @22:40:38.82 washCycleDA #1 of 2 @22:40:38.83 PSR.select :air @22:40:38.84 PSV.dial PSR @22:40:40.61 Pulling 0.5ml of PSR.wash @22:40:40.62 PSR.select :wash @22:40:40.73 PS.reconfigure :default @22:40:40.77 PS.seek 0.5ml @22:40:46.76 delay 5 seconds @22:40:51.78 PSR.select :air @22:40:51.89 PS.seek 2ml @22:41:07.85 PTV.dial :puck @22:41:08.57 PSV.dial PTV @22:41:08.76 Stroke completed -- waiting for pressure to equalize ... @22:41:08.78 SS.reconfigure :exhale @22:41:08.82 SS.hold @22:41:09.30 PBV.dial :waste1 @22:41:09.77 PS.seek 1.5ml @22:41:11.74 Intake.select :close @22:41:11.89 SSV.dial :exhaust @22:41:12.61 Exhaust.select :open @22:41:12.72 SS.seek 0ml @22:41:15.79 delay 3 seconds @22:41:18.80 PBV.dial between PSV and waste1 @22:41:19.17 PS.seek 0ml @22:41:21.70 Exhaust.select :close @22:41:21.81 SSV.dial CBV @22:41:21.84 CBV.dial SSV @22:41:22.60 Allowing 130s for 25.0ml of remaining 825.0ml @22:41:22.61 Intake.select :open @22:41:24.76 Intake @14.7psia, vacuum from 9.2 to 12.2psi @22:41:24.78 SS.reconfigure :inhaling 0.84ml/s @22:41:24.83 SS.seek 25ml @22:41:35.32 delay 2:00 @22:41:51.38 Vacuum too high -- waiting up to 40 seconds @22:41:51.39 SS.reconfigure :exhale @22:41:51.44 SS.hold @22:41:53.08 Intake @14.5psia, vacuum from 9.0 to 12.0psi @22:41:53.10 SS.reconfigure :inhaling 0.76ml/s @22:41:53.15 SS.seek 25ml @22:41:59.68 Stroke completed -- waiting for pressure to equalize ... @22:41:59.70 SS.reconfigure :exhale @22:41:59.74 SS.hold @22:42:01.79 Intake.select :close @22:42:01.93 SSV.dial :exhaust @22:42:02.66 Exhaust.select :open @22:42:02.77 SS.seek 0ml @22:42:12.15 Exhaust.select :close @22:42:12.26 SSV.dial CBV @22:42:12.31 CBV.dial SSV @22:42:13.00 Allowing 130s for 25.0ml of remaining 800.0ml @22:42:13.01 Intake.select :open @22:42:15.17 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:42:15.18 SS.reconfigure :inhaling 0.76ml/s @22:42:15.23 SS.seek 25ml @22:42:49.87 Stroke completed -- waiting for pressure to equalize ... @22:42:49.88 SS.reconfigure :exhale @22:42:49.92 SS.hold @22:42:52.42 Intake.select :close @22:42:52.56 SSV.dial :exhaust @22:42:53.29 Exhaust.select :open @22:42:53.40 SS.seek 0ml @22:43:02.42 Exhaust.select :close @22:43:02.52 SSV.dial CBV @22:43:02.55 CBV.dial SSV @22:43:03.30 Allowing 130s for 25.0ml of remaining 775.0ml @22:43:03.31 Intake.select :open @22:43:05.46 Intake @14.7psia, vacuum from 9.2 to 12.2psi @22:43:05.48 SS.reconfigure :inhaling 0.76ml/s @22:43:05.52 SS.seek 25ml @22:43:35.34 recoverPSRreagent08 @22:43:35.35 PTV.dial :puck @22:43:35.36 PBV.dial :waste1 @22:43:35.72 delay 30 seconds @22:43:40.88 Stroke completed -- waiting for pressure to equalize ... @22:43:40.90 SS.reconfigure :exhale @22:43:40.94 SS.hold @22:43:44.03 Intake.select :close @22:43:44.17 SSV.dial :exhaust @22:43:44.89 Exhaust.select :open @22:43:45.00 SS.seek 0ml @22:43:54.32 Exhaust.select :close @22:43:54.43 SSV.dial CBV @22:43:54.46 CBV.dial SSV @22:43:55.20 Allowing 130s for 25.0ml of remaining 750.0ml @22:43:55.21 Intake.select :open @22:43:57.37 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:43:57.39 SS.reconfigure :inhaling 0.76ml/s @22:43:57.43 SS.seek 25ml @22:44:05.74 PBV.dial between PSV and waste1 @22:44:06.10 PSR.select :air @22:44:06.11 PSV.dial PSR @22:44:06.84 PS.reconfigure :fast @22:44:06.89 PS.seek 2.5ml @22:44:26.25 Vacuum too high -- waiting up to 40 seconds @22:44:26.26 SS.reconfigure :exhale @22:44:26.31 SS.hold @22:44:28.35 Intake @14.3psia, vacuum from 8.8 to 11.8psi @22:44:28.37 SS.reconfigure :inhaling 0.69ml/s @22:44:28.42 SS.seek 25ml @22:44:29.69 PSV.dial PTV @22:44:30.41 delay 5 seconds @22:44:35.24 Stroke completed -- waiting for pressure to equalize ... @22:44:35.25 SS.reconfigure :exhale @22:44:35.30 SS.hold @22:44:35.42 PSV.dial PSR @22:44:36.15 delay 5 seconds @22:44:37.71 Intake.select :close @22:44:37.86 SSV.dial :exhaust @22:44:38.59 Exhaust.select :open @22:44:38.70 SS.seek 0ml @22:44:41.17 PSV.dial PTV @22:44:41.88 delay 5 seconds @22:44:46.89 PSV.dial :air @22:44:47.71 PS.seek 0ml @22:44:47.96 Exhaust.select :close @22:44:48.07 SSV.dial CBV @22:44:48.11 CBV.dial SSV @22:44:48.84 Allowing 130s for 25.0ml of remaining 725.0ml @22:44:48.85 Intake.select :open @22:44:51.01 Intake @14.7psia, vacuum from 9.2 to 12.2psi @22:44:51.02 SS.reconfigure :inhaling 0.69ml/s @22:44:51.07 SS.seek 25ml @22:45:10.47 washCycleDA #2 of 2 @22:45:10.48 PSR.select :air @22:45:10.49 PSV.dial PSR @22:45:11.79 Pulling 0.5ml of PSR.wash PSR.select :wash @22:45:11.91 PS.reconfigure :default @22:45:11.95 PS.seek 0.5ml @22:45:17.81 delay 5 seconds @22:45:22.83 PSR.select :air @22:45:22.94 PS.seek 2ml @22:45:29.22 Stroke completed -- waiting for pressure to equalize ... @22:45:29.24 SS.reconfigure :exhale @22:45:29.28 SS.hold @22:45:33.35 Intake.select :close @22:45:33.50 SSV.dial :exhaust @22:45:34.21 Exhaust.select :open @22:45:34.33 SS.seek 0ml @22:45:39.03 PTV.dial :puck @22:45:39.04 PSV.dial PTV @22:45:39.75 PBV.dial :waste1 @22:45:40.17 PS.seek 1.5ml @22:45:43.29 Exhaust.select :close @22:45:43.39 SSV.dial CBV @22:45:43.42 CBV.dial SSV @22:45:44.17 Allowing 130s for 25.0ml of remaining 700.0ml @22:45:44.18 Intake.select :open @22:45:46.09 delay 3 seconds @22:45:46.33 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:45:46.35 SS.reconfigure :inhaling 0.69ml/s @22:45:46.40 SS.seek 25ml @22:45:49.11 PBV.dial between PSV and waste1 @22:45:49.96 PS.seek 0ml @22:46:05.94 delay 2:00 @22:46:20.69 Vacuum too high -- waiting up to 40 seconds @22:46:20.70 SS.reconfigure :exhale @22:46:20.74 SS.hold @22:46:22.83 Intake @13.9psia, vacuum from 8.4 to 11.4psi @22:46:22.85 SS.reconfigure :inhaling 0.61ml/s @22:46:22.89 SS.seek 25ml @22:46:28.63 Stroke completed -- waiting for pressure to equalize ... @22:46:28.64 SS.reconfigure :exhale @22:46:28.68 SS.hold @22:46:31.58 Intake.select :close @22:46:31.73 SSV.dial :exhaust @22:46:32.46 Exhaust.select :open @22:46:32.57 SS.seek 0ml @22:46:41.97 Exhaust.select :close @22:46:42.08 SSV.dial CBV @22:46:42.11 CBV.dial SSV @22:46:42.85 Allowing 130s for 25.0ml of remaining 675.0ml @22:46:42.86 Intake.select :open @22:46:45.02 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:46:45.03 SS.reconfigure :inhaling 0.61ml/s @22:46:45.08 SS.seek 25ml @22:47:27.67 Stroke completed -- waiting for pressure to equalize ... @22:47:27.69 SS.reconfigure :exhale @22:47:27.73 SS.hold @22:47:31.89 Intake.select :close @22:47:32.03 SSV.dial :exhaust @22:47:32.76 Exhaust.select :open @22:47:32.87 SS.seek 0ml @22:47:42.21 Exhaust.select :close @22:47:42.32 SSV.dial CBV @22:47:42.35 CBV.dial SSV @22:47:43.08 Allowing 130s for 25.0ml of remaining 650.0ml @22:47:43.10 Intake.select :open @22:47:45.25 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:47:45.27 SS.reconfigure :inhaling 0.61ml/s @22:47:45.31 SS.seek 25ml @22:48:05.96 recoverPSRreagent08 @22:48:05.97 PTV.dial :puck @22:48:05.98 PBV.dial :waste1 @22:48:06.34 delay 30 seconds @22:48:20.09 Vacuum too high -- waiting up to 40 seconds @22:48:20.10 SS.reconfigure :exhale @22:48:20.14 SS.hold @22:48:23.02 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:48:23.04 SS.reconfigure :inhaling 0.56ml/s @22:48:23.08 SS.seek 25ml @22:48:32.81 Stroke completed -- waiting for pressure to equalize ... @22:48:32.82 SS.reconfigure :exhale @22:48:32.87 SS.hold @22:48:36.42 PBV.dial between PSV and waste1 @22:48:36.48 Intake.select :close @22:48:36.62 SSV.dial :exhaust @22:48:36.78 PSR.select :air @22:48:36.79 PSV.dial PSR @22:48:37.35 Exhaust.select :open @22:48:37.46 SS.seek 0ml @22:48:37.52 PS.reconfigure :fast @22:48:37.57 PS.seek 2.5ml @22:48:46.78 Exhaust.select :close @22:48:47.39 SSV.dial CBV @22:48:47.42 CBV.dial SSV @22:48:48.16 Allowing 130s for 25.0ml of remaining 625.0ml @22:48:48.17 Intake.select :open @22:48:50.33 Intake @14.7psia, vacuum from 9.2 to 12.2psi @22:48:50.35 SS.reconfigure :inhaling 0.56ml/s @22:48:50.39 SS.seek 25ml @22:49:00.34 PSV.dial PTV @22:49:01.17 delay 5 seconds @22:49:06.19 PSV.dial PSR @22:49:06.88 delay 5 seconds @22:49:11.90 PSV.dial PTV @22:49:12.62 delay 5 seconds @22:49:17.64 PSV.dial :air @22:49:18.34 PS.seek 0ml @22:49:37.86 Stroke completed -- waiting for pressure to equalize ... @22:49:37.87 SS.reconfigure :exhale @22:49:37.91 SS.hold @22:49:40.93 getPMLReagent08 @22:49:40.94 PRV.dial :air @22:49:41.37 delay 5 seconds @22:49:43.45 Intake.select :close @22:49:43.59 SSV.dial :exhaust @22:49:44.33 Exhaust.select :open @22:49:44.44 SS.seek 0ml @22:49:46.38 PTV.dial :PRVmixing @22:49:47.68 delay 5 seconds @22:49:52.70 PRV.dial :ab2 @22:49:53.13 PTV.dial :PRVmixing @22:49:53.14 PSV.dial :mixloop @22:49:53.70 PS.reconfigure :default @22:49:53.74 PS.seek 0.3ml @22:49:53.80 Exhaust.select :close @22:49:53.91 SSV.dial CBV @22:49:53.93 CBV.dial SSV @22:49:54.67 Allowing 130s for 25.0ml of remaining 600.0ml @22:49:54.69 Intake.select :open @22:49:56.84 Intake @14.9psia, vacuum from 9.4 to 12.4psi @22:49:56.86 SS.reconfigure :inhaling 0.56ml/s @22:49:56.90 SS.seek 25ml @22:49:57.58 delay 2 seconds @22:49:59.60 PRV.dial :air @22:50:00.11 PS.seek 0.55ml @22:50:03.56 addPMLReagent08 @22:50:03.57 PSV.dial :air @22:50:03.98 PS.seek 2ml @22:50:19.58 PSV.dial :PML @22:50:20.00 PTV.dial :mixing @22:50:20.74 PBV.dial :waste1 @22:50:21.14 PS.seek 1.85ml @22:50:23.52 PBV.dial between PSV and waste1 @22:50:23.89 PS.seek 0ml @22:50:27.58 Vacuum too high -- waiting up to 40 seconds @22:50:27.60 SS.reconfigure :exhale @22:50:27.64 SS.hold @22:50:30.67 Intake @14.7psia, vacuum from 9.2 to 12.2psi @22:50:30.69 SS.reconfigure :inhaling 0.50ml/s @22:50:30.74 SS.seek 25ml @22:50:43.46 delay 2 seconds @22:50:45.48 delay 8:00 @22:50:49.90 Stroke completed -- waiting for pressure to equalize ... @22:50:49.91 SS.reconfigure :exhale @22:50:49.96 SS.hold @22:50:54.88 Intake.select :close @22:50:55.03 SSV.dial :exhaust @22:50:55.75 Exhaust.select :open @22:50:55.86 SS.seek 0ml @22:51:04.89 Exhaust.select :close @22:51:04.99 SSV.dial CBV @22:51:05.02 CBV.dial SSV @22:51:05.76 Allowing 130s for 25.0ml of remaining 575.0ml @22:51:05.78 Intake.select :open @22:51:07.93 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:51:08.44 SS.reconfigure :inhaling 0.50ml/s @22:51:08.49 SS.seek 25ml @22:52:03.12 Stroke completed -- waiting for pressure to equalize ... @22:52:03.13 SS.reconfigure :exhale @22:52:03.17 SS.hold @22:52:10.56 Intake.select :close @22:52:10.70 SSV.dial :exhaust @22:52:11.43 Exhaust.select :open @22:52:11.54 SS.seek 0ml @22:52:20.57 Exhaust.select :close @22:52:20.68 SSV.dial CBV @22:52:20.73 CBV.dial SSV @22:52:21.43 Allowing 130s for 25.0ml of remaining 550.0ml @22:52:21.45 Intake.select :open @22:52:23.60 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:52:23.62 SS.reconfigure :inhaling 0.50ml/s @22:52:23.67 SS.seek 25ml @22:52:58.95 Vacuum too high -- waiting up to 40 seconds @22:52:58.97 SS.reconfigure :exhale @22:52:59.01 SS.hold @22:53:02.55 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:53:02.57 SS.reconfigure :inhaling 0.46ml/s @22:53:02.61 SS.seek 25ml @22:53:26.32 Stroke completed -- waiting for pressure to equalize ... @22:53:26.33 SS.reconfigure :exhale @22:53:26.37 SS.hold @22:53:32.91 Intake.select :close @22:53:33.05 SSV.dial :exhaust @22:53:33.77 Exhaust.select :open @22:53:33.88 SS.seek 0ml @22:53:42.87 Exhaust.select :close @22:53:42.97 SSV.dial CBV @22:53:43.00 CBV.dial SSV @22:53:43.74 Allowing 130s for 25.0ml of remaining 525.0ml @22:53:43.76 Intake.select :open @22:53:45.91 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:53:45.93 SS.reconfigure :inhaling 0.46ml/s @22:53:45.98 SS.seek 25ml @22:54:48.46 Stroke completed -- waiting for pressure to equalize ... @22:54:48.47 SS.reconfigure :exhale @22:54:48.51 SS.hold @22:54:58.17 Intake.select :close @22:54:58.31 SSV.dial :exhaust @22:54:59.04 Exhaust.select :open @22:54:59.15 SS.seek 0ml @22:55:08.46 Exhaust.select :close @22:55:08.56 SSV.dial CBV @22:55:08.59 CBV.dial SSV @22:55:09.32 Allowing 130s for 25.0ml of remaining 500.0ml @22:55:09.33 Intake.select :open @22:55:11.48 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:55:11.99 SS.reconfigure :inhaling 0.46ml/s @22:55:12.05 SS.seek 25ml @22:55:45.64 Vacuum too high -- waiting up to 40 seconds @22:55:45.65 SS.reconfigure :exhale @22:55:45.69 SS.hold @22:55:49.43 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:55:49.45 SS.reconfigure :inhaling 0.41ml/s @22:55:49.50 SS.seek 25ml @22:56:10.92 Vacuum too high -- waiting up to 40 seconds @22:56:10.93 SS.reconfigure :exhale @22:56:10.97 SS.hold @22:56:16.63 Intake @15.2psia, vacuum from 9.7 to 12.7psi @22:56:16.65 SS.reconfigure :inhaling 0.37ml/s @22:56:16.69 SS.seek 25ml @22:56:30.30 Stroke completed -- waiting for pressure to equalize ... @22:56:30.31 SS.reconfigure :exhale @22:56:30.35 SS.hold @22:56:37.72 Intake.select :close @22:56:37.86 SSV.dial :exhaust @22:56:38.58 Exhaust.select :open @22:56:38.69 SS.seek 0ml @22:56:48.00 Exhaust.select :close @22:56:48.11 SSV.dial CBV @22:56:48.16 CBV.dial SSV @22:56:48.86 Allowing 130s for 25.0ml of remaining 475.0ml @22:56:48.88 Intake.select :open @22:56:51.03 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:56:51.05 SS.reconfigure :inhaling 0.37ml/s @22:56:51.09 SS.seek 25ml @22:57:49.76 Vacuum too high -- waiting up to 40 seconds @22:57:49.77 SS.reconfigure :exhale @22:57:49.82 SS.hold @22:57:56.32 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:57:56.34 SS.reconfigure :inhaling 0.33ml/s @22:57:56.39 SS.seek 25ml @22:58:20.68 Stroke completed -- waiting for pressure to equalize ... @22:58:20.70 SS.reconfigure :exhale @22:58:20.74 SS.hold @22:58:31.86 Intake.select :close @22:58:32.00 SSV.dial :exhaust @22:58:32.73 Exhaust.select :open @22:58:32.84 SS.seek 0ml @22:58:42.39 Exhaust.select :close @22:58:42.50 SSV.dial CBV @22:58:42.52 CBV.dial SSV @22:58:43.27 Allowing 130s for 25.0ml of remaining 450.0ml @22:58:43.28 Intake.select :open @22:58:45.43 Intake @15.1psia, vacuum from 9.6 to 12.6psi @22:58:45.45 SS.reconfigure :inhaling 0.33ml/s @22:58:45.50 SS.seek 25ml @22:58:45.54 recoverPRVreagent08 @22:58:45.55 PTV.dial :mixing @22:58:45.56 PBV.dial :waste1 @22:58:45.92 delay 30 seconds @22:59:15.94 PBV.dial between PSV and waste1 @22:59:16.29 PSV.dial :air @22:59:16.75 PS.reconfigure :fast @22:59:16.79 PS.seek 2.5ml @22:59:24.50 Vacuum too high -- waiting up to 40 seconds @22:59:24.52 SS.reconfigure :exhale @22:59:24.56 SS.hold @22:59:31.08 SS.reconfigure :inhaling 0.30ml/s @22:59:31.12 SS.seek 25ml @22:59:39.50 PSV.dial :mixloop @22:59:39.96 delay 2 seconds @22:59:41.98 PSV.dial :air @22:59:42.43 delay 2 seconds @22:59:44.44 PSV.dial :mixloop @22:59:44.90 delay 2 seconds @22:59:46.91 PSV.dial :air @22:59:47.37 PS.seek 0ml @23:00:03.35 Vacuum too high -- waiting up to 40 seconds @23:00:03.36 SS.reconfigure :exhale @23:00:03.40 SS.hold @23:00:09.97 washCycleDA #1 of 3 @23:00:09.98 PSR.select :air @23:00:10.00 PSV.dial PSR @23:00:11.41 Pulling 0.5ml of PSR.wash @23:00:11.42 PSR.select :wash @23:00:11.54 PS.reconfigure :default @23:00:11.58 PS.seek 0.5ml @23:00:11.95 Intake @15.1psia, vacuum from 9.6 to 12.6psi @23:00:11.96 SS.reconfigure :inhaling 0.27ml/s @23:00:12.01 SS.seek 25ml @23:00:17.41 delay 5 seconds @23:00:22.43 PSR.select :air @23:00:22.55 PS.seek 2ml @23:00:38.53 PTV.dial :puck @23:00:39.27 PSV.dial PTV @23:00:39.99 PBV.dial :waste1 @23:00:40.42 PS.seek 1.5ml @23:00:41.18 Stroke completed -- waiting for pressure to equalize ... @23:00:41.19 SS.reconfigure :exhale @23:00:41.23 SS.hold @23:00:46.41 delay 3 seconds @23:00:49.42 PBV.dial between PSV and waste1 @23:00:49.81 PS.seek 0ml @23:00:53.29 SS.reconfigure :default @23:00:53.33 SS.hold @23:00:53.44 Intake.select :close @23:00:53.60 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 575.0ml @23:00:53.61 SSV.dial :exhaust @23:00:54.32 Exhaust.select :open @23:00:54.86 SS.reconfigure :exhale @23:00:54.99 SS.seek 0ml @23:01:03.99 Exhaust.select :close @23:01:04.10 Sampled 575.0ml @23:01:04.12 Drying puck before fixing for 20:00 @23:01:04.13 CBV.dial :waste2b @23:01:04.82 delay 1:00 @23:01:05.91 delay 2:00 @23:02:04.84 CSV.dial :air @23:02:05.28 CS.reconfigure :fast @23:02:05.32 CS.seek 10ml @23:02:27.74 Pumping wcr puck from tube 2 up to 35psi... @23:02:27.75 CBV.dial SSV @23:02:29.03 CBV.dial between CSV and waste2b @23:02:29.94 CSV.dial between air and CTV @23:02:30.37 CS.seek 7.653ml @23:02:36.27 CTV.dial CSV,avoiding: :air @23:02:36.30 CSV.dial CTV @23:02:37.07 CS.reconfigure :pressurize @23:02:37.12 CS.seek 0ml @23:02:55.13 CBV.dial :waste2b @23:02:55.46 CS.reconfigure :default @23:02:55.51 CS.seek 0ml @23:02:58.02 delay 1:00 @23:03:05.94 recoverPSRreagent08 @23:03:05.95 PTV.dial :puck @23:03:05.96 PBV.dial :waste1 @23:03:06.33 delay 30 seconds @23:03:36.35 PBV.dial between PSV and waste1 @23:03:36.72 PSR.select :air @23:03:36.74 PSV.dial PSR @23:03:37.46 PS.reconfigure :fast @23:03:37.50 PS.seek 2.5ml @23:03:58.04 CTV.dial :air,avoiding: :intake @23:03:59.31 Fix #1 of 2 (with RNAlater) for 10:00 @23:03:59.33 CSV.dial CSR @23:03:59.79 Pulling 2ml of CSR.RNAlater @23:03:59.80 CSR.select :RNAlater @23:03:59.91 CS.seek 2ml @23:04:00.28 PSV.dial PTV @23:04:01.00 delay 5 seconds @23:04:05.80 delay 5 seconds @23:04:06.01 PSV.dial PSR @23:04:06.74 delay 5 seconds @23:04:10.82 CSR.select :air @23:04:10.94 CS.reconfigure :fast @23:04:10.98 CS.seek 10ml @23:04:11.75 PSV.dial PTV @23:04:12.47 delay 5 seconds @23:04:17.49 PSV.dial :air @23:04:18.19 PS.seek 0ml @23:04:29.02 Pumping wcr puck from tube 2 up to 35psi... @23:04:29.04 CBV.dial SSV @23:04:30.30 CBV.dial between CSV and waste2b @23:04:31.22 CSV.dial between air and CTV @23:04:31.81 CS.seek 7.319ml @23:04:38.43 CTV.dial CSV,avoiding: :air @23:04:38.46 CSV.dial CTV @23:04:39.75 CS.reconfigure :pressurize @23:04:39.80 CS.seek 0ml @23:04:40.81 washCycleDA #2 of 3 @23:04:40.82 PSR.select :air @23:04:40.84 PSV.dial PSR @23:04:42.13 Pulling 0.5ml of PSR.wash @23:04:42.14 PSR.select :wash @23:04:42.25 PS.reconfigure :default @23:04:42.29 PS.seek 0.5ml @23:04:48.19 delay 5 seconds @23:04:53.21 PSR.select :air @23:04:53.32 PS.seek 2ml @23:04:56.91 delay 10:00 @23:05:09.34 PTV.dial :puck @23:05:09.35 PSV.dial PTV @23:05:10.04 PBV.dial :waste1 @23:05:10.44 PS.seek 1.5ml @23:05:16.40 delay 3 seconds @23:05:19.42 PBV.dial between PSV and waste1 @23:05:19.79 PS.seek 0ml @23:05:35.84 delay 2:00 @23:07:35.87 recoverPSRreagent08 @23:07:35.88 PTV.dial :puck @23:07:35.89 PBV.dial :waste1 @23:07:36.24 delay 30 seconds @23:08:06.26 PBV.dial between PSV and waste1 @23:08:06.62 PSR.select :air @23:08:07.11 PSV.dial PSR @23:08:07.84 PS.reconfigure :fast @23:08:07.89 PS.seek 2.5ml @23:08:30.74 PSV.dial PTV @23:08:31.57 delay 5 seconds @23:08:36.59 PSV.dial PSR @23:08:37.29 delay 5 seconds @23:08:42.31 PSV.dial PTV @23:08:43.02 delay 5 seconds @23:08:48.04 PSV.dial :air @23:08:48.75 PS.seek 0ml @23:09:11.21 washCycleDA #3 of 3 @23:09:11.22 PSR.select :air @23:09:11.23 PSV.dial PSR @23:09:12.53 Pulling 0.5ml of PSR.wash @23:09:12.54 PSR.select :wash @23:09:12.65 PS.reconfigure :default @23:09:12.69 PS.seek 0.5ml @23:09:18.68 delay 5 seconds @23:09:23.70 PSR.select :air @23:09:23.81 PS.seek 2ml @23:09:39.83 PTV.dial :puck @23:09:39.84 PSV.dial PTV @23:09:40.54 PBV.dial :waste1 @23:09:40.95 PS.seek 1.5ml @23:09:46.86 delay 3 seconds @23:09:49.88 PBV.dial between PSV and waste1 @23:09:50.25 PS.seek 0ml @23:10:06.27 delay 2:00 @23:12:06.30 recoverPSRreagent08 PTV.dial :puck @23:12:06.31 PBV.dial :waste1 @23:12:06.66 delay 30 seconds @23:12:36.68 PBV.dial between PSV and waste1 @23:12:37.03 PSR.select :air @23:12:37.05 PSV.dial PSR @23:12:37.77 PS.reconfigure :fast @23:12:37.82 PS.seek 2.5ml @23:13:00.42 PSV.dial PTV @23:13:01.14 delay 5 seconds @23:13:06.16 PSV.dial PSR @23:13:06.87 delay 5 seconds @23:13:11.89 PSV.dial PTV @23:13:12.61 delay 5 seconds @23:13:17.63 PSV.dial :air @23:13:18.33 PS.seek 0ml @23:13:40.92 Gate.power :camera,:ON @23:13:40.93 Mixing DA Substrates @23:13:40.94 PSR.select :air @23:13:40.96 PSV.dial PSR @23:13:42.60 Pulling 0.25ml of PSR.sub1 @23:13:42.63 PSR.select :sub1 @23:13:42.90 PS.reconfigure :default @23:13:43.09 PS.seek 0.25ml @23:13:46.49 delay 5 seconds @23:13:51.58 Pulling 0.25ml of PSR.sub2 @23:13:51.59 PSR.select :sub2 @23:13:51.70 PS.seek 0.5ml @23:13:55.05 delay 5 seconds @23:14:00.07 PSR.select :air @23:14:00.17 PS.seek 2ml @23:14:16.20 PTV.dial :puck @23:14:16.21 PSV.dial PTV @23:14:16.94 PBV.dial :waste1 @23:14:17.37 PS.seek 1.5ml @23:14:23.36 delay 3 seconds @23:14:26.37 PBV.dial between PSV and waste1 @23:14:26.74 PS.seek 0ml @23:14:42.79 delay 10 seconds @23:14:52.81 recoverPSRreagent08 @23:14:52.82 PTV.dial :puck @23:14:52.84 PBV.dial :waste1 @23:14:53.18 delay 30 seconds @23:14:56.93 CBV.dial :waste2b @23:14:57.27 CS.reconfigure :default @23:14:57.32 CS.seek 0ml @23:14:59.81 delay 1:00 @23:15:23.20 PBV.dial between PSV and waste1 @23:15:23.56 PSR.select :air @23:15:23.58 PSV.dial PSR @23:15:24.30 PS.reconfigure :fast @23:15:24.34 PS.seek 2.5ml @23:15:47.01 PSV.dial PTV @23:15:47.73 delay 5 seconds @23:15:52.75 PSV.dial PSR @23:15:53.46 delay 5 seconds @23:15:58.48 PSV.dial PTV @23:15:59.20 delay 5 seconds @23:15:59.83 CTV.dial :air,avoiding: :intake @23:16:01.08 Fix #2 of 2 (with RNAlater) for 10:00 @23:16:01.10 CSV.dial CSR @23:16:01.57 Pulling 2ml of CSR.RNAlater @23:16:01.58 CSR.select :RNAlater @23:16:01.69 CS.seek 2ml @23:16:04.22 PSV.dial :air @23:16:04.94 PS.seek 0ml @23:16:07.56 delay 5 seconds @23:16:12.58 CSR.select :air @23:16:12.69 CS.reconfigure :fast @23:16:12.73 CS.seek 10ml @23:16:27.57 Additional air clear @23:16:27.58 PSR.select :air @23:16:27.60 PSV.dial PSR @23:16:28.89 Pulling 2ml of PSR.air @23:16:28.90 PSR.select :air @23:16:28.92 PS.reconfigure :default @23:16:28.96 PS.seek 2ml @23:16:31.09 Pumping wcr puck from tube 2 up to 35psi... @23:16:31.11 CBV.dial SSV @23:16:32.88 CBV.dial between CSV and waste2b @23:16:33.81 CSV.dial between air and CTV @23:16:34.40 CS.seek 7.243ml @23:16:41.28 CTV.dial CSV,avoiding: :air @23:16:41.31 CSV.dial CTV @23:16:42.74 CS.reconfigure :pressurize @23:16:42.79 CS.seek 0ml @23:16:50.07 delay 5 seconds @23:16:55.09 PTV.dial :puck @23:16:55.11 PSV.dial PTV @23:16:55.93 PBV.dial :waste1 @23:16:56.35 PS.seek 1.5ml @23:16:59.55 delay 10:00 @23:17:02.29 delay 3 seconds @23:17:05.31 PBV.dial between PSV and waste1 @23:17:05.67 delay 1 second @23:17:06.68 PSV.dial :waste1 @23:17:07.65 PS.seek 0ml @23:17:23.74 Processing Puck Heater at 28C before imaging @23:17:23.75 PH.coast @23:17:23.87 Imaging da puck in Processing Clamp while storing it to tube 1 @23:17:24.04 SC.reconfigure :tight @23:17:24.08 SC.seek 1 @23:17:27.74 SC.configure :limp @23:17:27.94 Elbow.reconfigure :tight @23:17:27.98 Elbow.seek 1 @23:17:29.64 Forearm.reconfigure :tight @23:17:29.69 Forearm.seek 1 + 700 counts @23:17:30.73 SE.reconfigure :pick @23:17:30.78 SE.seek :beamBreak @23:17:33.03 SE.configure :default @23:17:33.17 Elbow.reconfigure :limp @23:17:33.22 Forearm.reconfigure :loose @23:17:33.26 Forearm.seek 1 @23:17:33.69 Elbow.hold @23:17:33.75 Elbow.reconfigure :tight @23:17:33.84 SE.seek 13.3 Pucks below Top Plate @23:17:34.30 Forearm.force -20 @23:17:34.34 Forearm.seek 1 + 313 counts @23:17:34.62 Forearm.reconfigure :tight @23:17:34.66 Forearm.seek 1 + 700 counts @23:17:34.94 SE.seek 14.3 Pucks below Top Plate @23:17:35.52 Forearm.reconfigure :loose @23:17:35.56 Forearm.seek :retract @23:17:36.58 Elbow.seek PC @23:17:39.21 Elbow.reconfigure :loose @23:17:39.30 Forearm.seek PC @23:17:40.33 Hand.close @23:17:40.94 PC.seek :open @23:17:44.95 Forearm.seek :retract @23:17:46.05 Elbow.reconfigure :tight @23:17:46.09 Elbow.seek 1 @23:17:48.69 Forearm.reconfigure :tight @23:17:48.74 Forearm.seek 1 + 700 counts @23:17:49.70 Hand.open @23:17:50.50 SE.seek 13.94 Pucks below Top Plate @23:17:50.76 SE.reconfigure :seal @23:17:50.81 SE.seek 12.84 Pucks below Top Plate @23:17:51.36 Lights, camera, ACTION! @23:17:51.39 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22oct1923h800ml.tif @23:18:06.98 SX MX5/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 12.054 seconds /var/log/esp/da22oct1923h800ml.tif: TIFF Upload Complete @23:18:07.01 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22oct1923h800ml15s.tif @23:18:10.85 Waiting >20 seconds for da to relinquish FlushPuck @23:18:22.79 SX MX5/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22oct1923h800ml15s.tif: TIFF Upload Complete @23:18:22.81 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da22oct1923h800ml25s.tif @23:18:48.57 SX MX5/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da22oct1923h800ml25s.tif: TIFF Upload Complete @23:18:48.58 Gate.power :camera,:OFF @23:18:48.66 DAfans.email "12.054 sec auto-exposure",Subject: "da22oct1923h800ml images!" @23:18:48.91 Elbow.seek 1 @23:18:49.08 Forearm.seek 1 + 700 counts @23:18:49.15 SE.reconfigure :gently @23:18:49.19 SE.seek 13.55 Pucks below Top Plate @23:18:49.99 Elbow.reconfigure :limp @23:18:50.04 Forearm.reconfigure :loose @23:18:50.08 Forearm.seek 1 @23:18:50.48 Elbow.hold @23:18:50.55 Elbow.reconfigure :tight @23:18:50.60 SE.reconfigure :default @23:18:50.65 SE.seek 13.74 Pucks below Top Plate @23:18:51.03 Forearm.force -20 @23:18:51.07 Forearm.seek 1 + 320 counts @23:18:51.40 Forearm.reconfigure :tight @23:18:51.46 Forearm.seek 1 + 700 counts @23:18:51.90 SE.seek :down @23:18:53.03 Forearm.reconfigure :loose @23:18:53.08 Forearm.seek :retract @23:18:53.81 SE.stop @23:18:54.08 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @23:18:54.26 Elbow.seek FlushPuck::Garage @23:18:56.28 Elbow.reconfigure :loose @23:18:56.38 Forearm.seek :garage @23:18:57.55 Hand.close @23:18:58.44 Forearm.seek :retract @23:18:59.67 Elbow.reconfigure :tight @23:18:59.77 Elbow.seek PC @23:19:01.36 Elbow.reconfigure :loose @23:19:01.58 Forearm.reconfigure :tight @23:19:01.67 Forearm.seek PC @23:19:03.15 PC.seek :closed @23:19:07.14 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.44 @23:19:07.29 Hand.open @23:19:08.23 Forearm.reconfigure :loose @23:19:08.30 Forearm.seek :retract @23:19:09.46 PSR.select :air @23:19:09.50 PSV.dial PSR @23:19:10.03 Pulling 0.5ml of PSR.flush @23:19:10.04 PSR.select :flush @23:19:10.16 PS.seek 0.5ml @23:19:16.10 delay 5 seconds @23:19:21.12 PSR.select :air @23:19:21.24 PS.seek 2.5ml @23:19:42.42 delay 1 second @23:19:43.46 PRV.dial :waste2,avoiding: :ab2 @23:19:45.27 PTV.dial :PRVmixing @23:19:46.55 PSV.dial :mixloop @23:19:47.71 PS.seek :empty @23:20:13.91 PSR.select :air @23:20:13.93 PSV.dial PSR @23:20:15.01 Pulling 0.5ml of PSR.flush @23:20:15.05 PSR.select :flush @23:20:15.21 PS.seek 0.5ml @23:20:21.19 delay 5 seconds @23:20:26.21 PSR.select :air @23:20:26.39 PS.seek 2.5ml @23:20:47.55 delay 1 second @23:20:48.58 PBV.dial :waste1 @23:20:48.95 PTV.dial :mixing @23:20:49.70 PSV.dial :mixloop @23:20:50.69 PS.seek :empty @23:21:16.86 PSV.dial PSR @23:21:17.91 PSR.select :flush @23:21:18.14 PS.seek 1ml @23:21:29.16 PSR.select :air @23:21:29.33 PS.seek 2.5ml @23:21:46.49 PSV.dial PTV @23:21:47.24 PTV.dial :puck @23:21:48.05 PBV.dial :waste1 @23:21:48.09 PS.seek 0ml @23:21:58.61 upsync @23:22:14.40 PSV.dial PSR @23:22:15.11 PSR.select :flush @23:22:15.22 PS.seek 0.5ml @23:22:21.06 PSR.select :air @23:22:21.17 PS.seek 2.5ml @23:22:42.34 PSV.dial PTV @23:22:43.04 PBV.dial PSV @23:22:43.59 PS.seek 0ml @23:23:09.78 PSV.dial PBV @23:23:10.22 PS.seek 2.5ml @23:23:36.40 PSV.dial :waste1 @23:23:37.11 PS.seek 0ml @23:24:03.31 PSV.dial PSR @23:24:03.75 PSR.select :air @23:24:03.77 PS.seek 2.5ml @23:24:29.90 PSV.dial PTV @23:24:30.60 PBV.dial PSV @23:24:30.62 PS.seek 0ml @23:24:56.87 PSV.dial PBV @23:24:57.31 PS.seek 2.5ml @23:25:23.43 PSV.dial :waste1 @23:25:24.14 PS.seek 0ml @23:25:50.37 PSV.dial PSR @23:25:50.80 PSR.select :flush @23:25:50.92 PS.seek 1ml @23:26:01.96 PSR.select :air @23:26:02.07 PS.seek 2.5ml @23:26:18.15 PSV.dial PTV @23:26:18.85 PTV.dial :puck @23:26:18.86 PBV.dial :waste1 @23:26:19.39 PS.seek 0ml @23:26:45.58 PSV.dial PSR @23:26:46.41 PSR.select :flush @23:26:46.53 PS.seek 0.5ml @23:26:52.43 PSR.select :air @23:26:52.54 PS.seek 2.5ml @23:26:59.57 CBV.dial :waste2b @23:26:59.89 CS.reconfigure :default @23:26:59.94 CS.seek 0ml @23:27:02.64 delay 1:00 @23:27:13.71 PSV.dial PTV @23:27:14.40 PBV.dial PSV @23:27:14.95 PS.seek 0ml @23:27:41.11 PSV.dial PBV @23:27:41.56 PS.seek 2.5ml @23:28:02.66 CTV.dial :air,avoiding: :intake @23:28:03.99 Storing wcr puck in Collection Clamp to tube 1 @23:28:04.15 SC.reconfigure :tight @23:28:04.20 SC.seek 1 @23:28:04.29 SC.configure :limp @23:28:04.49 Elbow.reconfigure :tight @23:28:04.53 Elbow.seek 1 @23:28:07.11 Forearm.reconfigure :tight @23:28:07.16 Forearm.seek 1 + 700 counts @23:28:07.71 PSV.dial :waste1 @23:28:08.18 SE.reconfigure :pick @23:28:08.23 SE.seek :beamBreak @23:28:08.42 PS.seek 0ml @23:28:10.44 SE.configure :default @23:28:10.58 Elbow.reconfigure :limp @23:28:10.63 Forearm.reconfigure :loose @23:28:10.67 Forearm.seek 1 @23:28:11.06 Elbow.hold @23:28:11.12 Elbow.reconfigure :tight @23:28:11.22 SE.seek 14.31 Pucks below Top Plate @23:28:11.81 Forearm.force -20 @23:28:11.85 Forearm.seek 1 + 316 counts @23:28:12.25 Forearm.reconfigure :tight @23:28:12.78 Forearm.seek 1 + 700 counts @23:28:13.05 SE.seek 15.31 Pucks below Top Plate @23:28:13.68 Forearm.reconfigure :loose @23:28:13.73 Forearm.seek :retract @23:28:14.73 Elbow.seek CC @23:28:16.35 Elbow.reconfigure :loose @23:28:16.44 Forearm.seek CC @23:28:17.43 Hand.close @23:28:18.04 CC.seek :open @23:28:22.51 Forearm.seek :retract @23:28:23.48 Elbow.reconfigure :tight @23:28:23.53 Elbow.seek 1 @23:28:25.28 Elbow.reconfigure :limp @23:28:25.33 Forearm.seek 1 @23:28:26.45 Elbow.hold @23:28:26.52 Elbow.reconfigure :tight @23:28:26.56 Hand.open @23:28:27.40 Forearm.force -20 @23:28:27.45 Forearm.seek 1 + 317 counts @23:28:27.74 Forearm.reconfigure :tight @23:28:27.79 Forearm.seek 1 + 700 counts @23:28:28.18 SE.seek :down @23:28:29.31 Forearm.reconfigure :loose @23:28:29.36 Forearm.seek :retract @23:28:29.87 SE.stop @23:28:30.34
Starting finalPurge @23:28:34.55 PSV.dial PSR @23:28:34.99 PSR.select :air @23:28:35.01 PS.seek 2.5ml @23:28:49.91
Waiting >20 seconds for psFlush to relinquish FlushPuck @23:29:01.20 PSV.dial PTV @23:29:01.91 PBV.dial PSV @23:29:01.93 PS.seek 0ml @23:29:28.14 PSV.dial PBV @23:29:28.59 PS.seek 2.5ml @23:29:54.73 PSV.dial :waste1 @23:29:55.44 PS.seek 0ml @23:30:21.71
Loading FlushPuck into Collection Clamp from Processing Clamp @23:30:21.88 Elbow.seek PC @23:30:24.34 Elbow.reconfigure :loose @23:30:24.43 Forearm.seek PC @23:30:25.50 Hand.close @23:30:26.16 PC.seek :open @23:30:30.12 Forearm.seek :retract @23:30:31.18 Elbow.reconfigure :tight @23:30:31.22 Elbow.seek CC @23:30:34.84 Elbow.reconfigure :loose @23:30:34.93 Forearm.reconfigure :tight @23:30:34.97 Forearm.seek CC @23:30:36.29 CC.seek :closed @23:30:40.54 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.84 @23:30:40.60 Hand.open @23:30:41.34 Forearm.reconfigure :loose @23:30:41.39 Forearm.seek :retract @23:30:42.38 Flushing the Collection Valve::Manifold @23:30:42.50 CSV.dial CSR @23:30:42.97 Pulling 5ml of CSR.flush CSR.select :flush @23:30:43.09 CS.seek 5ml @23:30:56.48 delay 5 seconds @23:31:01.49 CSR.select :air @23:31:02.11 CS.reconfigure :fast @23:31:02.17 CS.seek 7.5ml @23:31:08.50 CSV.dial :waste @23:31:09.74 CS.reconfigure :default @23:31:09.79 CS.seek 0ml @23:31:29.54 Flushing lower manifold's waste1 port @23:31:29.65 CSV.dial :flush @23:31:30.09 CS.reconfigure :fast @23:31:30.13 CS.seek 5ml @23:31:41.82 delay 5 seconds @23:31:46.83 CSV.dial :air @23:31:47.44 CS.seek 7ml @23:31:52.70 CSV.dial CTV @23:31:52.72
CTV.dial CSV @23:31:54.00 CBV.dial :waste1 @23:31:54.74 CS.seek 0ml @23:32:10.50 Flushing lower manifold's waste2 port @23:32:10.62 CSV.dial :flush @23:32:11.34 CS.seek 5ml @23:32:22.91 delay 5 seconds @23:32:27.93 CSV.dial :air @23:32:28.44 CS.seek 7ml @23:32:33.60 CSV.dial CTV @23:32:33.63
CTV.dial CSV @23:32:34.05 CBV.dial :waste2 @23:32:35.05 CS.seek 0ml @23:32:50.78 Flushing lower manifold's waste2b port @23:32:50.90 CSV.dial :flush @23:32:51.62 CS.seek 5ml @23:33:03.22 delay 5 seconds @23:33:08.24 CSV.dial :air @23:33:08.75 CS.seek 7ml @23:33:13.94 CSV.dial CTV @23:33:13.97
CTV.dial CSV @23:33:14.39 CBV.dial :waste2b @23:33:15.38 CS.seek 0ml @23:33:31.22 Disinfecting external Intake with kill @23:33:31.33 CSV.dial CSR @23:33:32.04 Pulling 8ml of CSR.kill @23:33:32.05 CSR.select :kill @23:33:32.16 CS.seek 8ml @23:33:50.31 delay 5 seconds @23:33:55.33 CSR.select :air @23:33:55.44 CS.seek 10ml @23:34:00.63 CSV.dial CBV @23:34:00.67
CBV.dial CSV @23:34:01.65 CTV.dial :intake @23:34:02.36 Intake.select :open @23:34:02.46 CS.seek 2ml @23:34:20.43 Intake.select :close @23:34:20.53 CTV.dial :air @23:34:21.27 CS.reconfigure :default @23:34:21.31 CS.seek 0ml @23:34:27.21 CSV.dial CSR @23:34:28.24 Pulling 5ml of CSR.flush @23:34:28.25 CSR.select :flush @23:34:28.36 CS.seek 5ml @23:34:41.78 delay 5 seconds @23:34:46.80 CSR.select :air @23:34:46.90 CS.seek 10ml @23:35:00.37 CSV.dial CBV @23:35:01.35 CS.seek 0ml @23:35:27.40 Garaging FlushPuck in Collection Clamp @23:35:27.47 Elbow.reconfigure :tight @23:35:27.51 Elbow.seek CC @23:35:27.63 Elbow.reconfigure :loose @23:35:27.72 Forearm.seek CC @23:35:28.65 Hand.close @23:35:29.28 CC.seek :open @23:35:33.60 Forearm.seek :retract @23:35:34.61 Elbow.reconfigure :tight @23:35:34.65 Elbow.seek :garage @23:35:37.77 Elbow.reconfigure :loose @23:35:37.85 Forearm.reconfigure :tight @23:35:37.90 Forearm.seek :garage @23:35:39.06 Hand.open @23:35:39.83 Forearm.reconfigure :loose @23:35:39.88 Forearm.seek :retract @23:35:40.92 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 21:05:49 @23:35:40.96 Asleep after completing DA_WCR_101922 assays @23:35:40.98 GoodNews.email "{1 => 16,\n 2 => 17,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@23:34:49, 25.6C, 19% humidity, 15.8psia, 14.011V, 0.410A, 0.410A avg, 5.75W\nSampled 575.0ml",Subject: "Asleep after completing DA_WCR_101922 assays" @23:36:16.47
Uploading Logs @23:37:33.04 upsync @16:16:11.95UTC28-Oct-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @16:16:14.67 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @16:16:18.76 Polling Can every 10:00 @16:16:18.78 Cleanly exited at 16:13:13UTC28-Oct-22 @16:16:18.82 Loading /home/esp/esp2/mission/DA_WCR_102822.rb mission @16:16:18.87 GoodNews.email "Starting DA_WCR_102822 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@16:16:18, 24.0C, 22% humidity, 15.7psia, 14.046V, 0.275A, 0.275A avg, 3.87W",Subject: "DA_WCR_102822 begun" @16:16:18.89
Preparing to sleep until + 00:00:05 @16:16:18.94 Asleep til + 00:00:05 @16:16:18.96 Continuing at 16:16 @16:16:19.25 Gate.power :core,:OFF @16:16:19.26 Gate.power :raw,:OFF @16:16:19.27 Shutdown @16:16:23.85
Powering core bus @16:16:23.92 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_102822.rb:14\n{1 => 16,\n 2 => 17,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:16:18, 24.0C, 22% humidity, 15.7psia, 14.046V, 0.275A, 0.275A avg, 3.87W",Subject: "Asleep until 16:16" @16:16:29.37
Gate.power :raw,:ON @16:16:30.85 CS.configure :default @16:16:31.03 PS.configure :default @16:16:31.29 SS.configure :default @16:16:31.47 CC.configure :default @16:16:31.69 PC.configure :default @16:16:31.92 Forearm.configure :loose @16:16:32.09 Elbow.configure :loose @16:16:32.30 SC.configure :loose @16:16:32.48 SE.configure :default @16:16:32.59 CH.coast @16:16:32.73 CH.configure :default @16:16:32.81 PH.coast @16:16:32.91 PH.configure :default @16:16:32.99 All dwarf controllers running firmware version 3.85 @16:16:33.13 Valve.reset @16:16:33.68 Intake.select :close @16:16:33.72 PSV.dial :air @16:16:33.76 Exhaust.select :close @16:16:33.80 CTV.dial :air @16:16:34.03 CSV.dial :waste1 @16:16:34.27
Forearm.reconfigure :loose @16:16:34.35 Forearm.home.seek :retract @16:16:34.63 SSV.dial :spit,avoiding: :exhaust @16:16:34.88 PS.reconfigure :default @16:16:34.98 PS.home.seek 0ml @16:16:35.16 Hand.close @16:16:35.22
Elbow.jog 1500 @16:16:35.54 CS.reconfigure :fast @16:16:35.60 SS.reconfigure :default @16:16:35.74 CS.home.seek 0ml @16:16:35.84 SS.home.seek 0ml @16:16:36.08
Elbow.reconfigure :loose @16:16:36.18 Elbow.home.seek :clear @16:16:39.47 SE.reconfigure :default @16:16:39.57 SE.home.seek :down @16:16:40.20 SC.reconfigure :tight @16:16:40.30 SC.home.seek :initial @16:16:43.62 PS.stop @16:16:59.19
SC.configure :limp @16:16:59.35 Forearm.seek :retract @16:16:59.45 Elbow.seek :hammer @16:17:00.65 Forearm.reconfigure :tight @16:17:00.73 Forearm.seek retracted @16:17:01.19 PC.reconfigure :default @16:17:01.25
CC.reconfigure :default @16:17:01.39 PC.home.seek :open @16:17:01.45
CC.home.seek :open @16:17:05.28 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @16:17:05.30 Notice.email "At 16:17:05 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_102822.rb:14\n{1 => 16,\n 2 => 17,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:17:05, 24.1C, 22% humidity, 15.7psia, 14.050V, 0.269A, 0.269A avg, 3.78W",Subject: "Started DA_WCR_102822 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @16:17:05.34
Starting initialPurge @16:17:05.40 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:17:05.51 Hand.open @16:17:06.57 Elbow.reconfigure :tight @16:17:06.65 Elbow.seek FlushPuck::Garage @16:17:08.67 Elbow.reconfigure :loose @16:17:08.87 Forearm.reconfigure :loose @16:17:08.94 Forearm.seek :garage @16:17:10.06 Hand.close @16:17:10.93 Forearm.seek :retract @16:17:12.00 Elbow.reconfigure :tight @16:17:12.10 Elbow.seek CC @16:17:15.06 Elbow.reconfigure :loose @16:17:15.24 Forearm.reconfigure :tight @16:17:15.44 Forearm.seek CC @16:17:17.05 CC.seek :closed @16:17:21.38 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.84 @16:17:21.56 Hand.open @16:17:22.47 Forearm.reconfigure :loose @16:17:22.55 Forearm.seek :retract @16:17:23.58 Flushing the Collection Valve::Manifold @16:17:23.80 CSV.dial CSR @16:17:25.19 Pulling 5ml of CSR.flush @16:17:25.21 CSR.select :flush @16:17:25.37 CS.reconfigure :default @16:17:25.45 CS.seek 5ml @16:17:38.88 delay 5 seconds @16:17:43.89 CSR.select :air @16:17:44.12 CS.reconfigure :fast @16:17:44.20 CS.seek 7.5ml @16:17:50.52 CSV.dial :waste @16:17:51.82 CS.reconfigure :default @16:17:51.90 CS.seek 0ml @16:18:11.60 Purging external Intake @16:18:11.82 CSV.dial :flush @16:18:12.32 CS.reconfigure :fast @16:18:12.40 CS.seek 10ml @16:18:18.99 upsync @16:18:35.22
delay 5 seconds @16:18:40.24 CSV.dial CBV @16:18:40.28
CBV.dial CSV @16:18:41.01 CTV.dial :intake @16:18:41.73 Intake.select :open @16:18:41.84 CS.seek 0ml @16:19:04.34 Intake.select :close @16:19:04.44 CTV.dial :air @16:19:05.17 Flushing upper manifold @16:19:05.29 CSV.dial :flush @16:19:06.01 CS.seek 5ml @16:19:17.75 delay 5 seconds @16:19:22.77 CSV.dial :air @16:19:23.26 CS.seek 7.001ml @16:19:28.54 CSV.dial CBV @16:19:28.56
CBV.dial CSV @16:19:29.67 CTV.dial :air @16:19:29.69 CS.seek 0ml @16:19:45.68 delay 7 seconds @16:19:52.71 CS.seek 3ml @16:20:00.06 CSV.dial :waste @16:20:00.49 CS.seek 0ml @16:20:08.01 CSV.dial :flush @16:20:08.44 CS.seek 3ml @16:20:15.84 delay 5 seconds @16:20:20.86 CSV.dial :air @16:20:21.34 CS.seek 5.001ml @16:20:26.50 CSV.dial :air @16:20:26.52 CS.seek 0ml @16:20:38.18 delay 7 seconds @16:20:45.21 CS.seek 3ml @16:20:52.65 CSV.dial :waste @16:20:53.36 CS.seek 0ml @16:21:00.78 Garaging FlushPuck in Collection Clamp @16:21:00.85 Elbow.reconfigure :tight @16:21:00.89 Elbow.seek CC @16:21:01.00 Elbow.reconfigure :loose @16:21:01.09 Forearm.seek CC @16:21:02.07 Hand.close @16:21:02.68 CC.seek :open @16:21:07.01 Forearm.seek :retract @16:21:08.09 Elbow.reconfigure :tight @16:21:08.13 Elbow.seek :garage @16:21:11.26 Elbow.reconfigure :loose @16:21:11.35 Forearm.reconfigure :tight @16:21:11.39 Forearm.seek :garage @16:21:12.52 Hand.open @16:21:13.70 Forearm.reconfigure :loose @16:21:13.81 Forearm.seek :retract @16:21:14.87 da sampling at most 1000ml, wcr at most 1000ml @16:21:14.90 Loading sh1 puck from tube 2 into Collection Clamp @16:21:14.92 DAfans.email "Started da in tube 2 at 16:21:14UTC28-Oct-22\n{1 => 16,\n 2 => 17,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:17:05, 24.1C, 22% humidity, 15.7psia, 14.050V, 0.269A, 0.269A avg, 3.78W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @16:21:15.16
SC.reconfigure :tight @16:21:15.20 SC.seek 2 @16:21:28.29 SC.configure :limp @16:21:28.74 Elbow.reconfigure :tight @16:21:28.82 Elbow.seek 2 @16:21:30.97 Forearm.reconfigure :tight @16:21:31.05 Forearm.seek 2 + 700 counts @16:21:32.20 SE.reconfigure :pick @16:21:32.31 SE.seek :beamBreak @16:21:34.52 SE.configure :default @16:21:34.81 Elbow.seek 2 @16:21:35.45 Forearm.seek 2 + 300 counts @16:21:35.70 Forearm.reconfigure :loose @16:21:35.78 Hand.close @16:21:36.48 SE.seek :down @16:21:36.90
Forearm.seek :retract @16:21:37.96 Elbow.seek CC @16:21:38.43 SE.stop @16:21:39.72
Elbow.reconfigure :loose @16:21:39.98 Forearm.reconfigure :tight @16:21:40.06 Forearm.seek CC @16:21:41.64 CC.seek :closed @16:21:46.08 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.69 @16:21:46.10 Hand.open @16:21:46.96 Forearm.reconfigure :loose @16:21:47.04 Forearm.seek :retract @16:21:48.04 collectSample 1000ml w/bubble point of 24psi @16:21:48.06 Priming 7ml @16:21:48.08 Intake.select :close @16:21:48.10 Exhaust.select :close @16:21:48.12 SS.hold @16:21:48.97 Calibrating pressure and temperature sensors @16:21:49.03 CTV.dial :air @16:21:49.05 SSV.dial :spit,avoiding: :exhaust @16:21:49.09 SS.reconfigure :fast @16:21:49.17 SS.seek 5ml @16:21:52.40 SSV.dial CBV @16:21:52.44
CBV.dial SSV @16:21:53.90 delay 3.8 seconds @16:21:57.93 SSV.dial :spit @16:21:59.24 SS.seek 0ml @16:22:02.07 SSV.dial CBV @16:22:03.38 CTV.dial :intake @16:22:04.17 SSV.dial CBV @16:22:04.21
CBV.dial SSV @16:22:04.23 Allowing 40s for 7.0ml of remaining 7.0ml @16:22:04.25 Intake.select :open @16:22:06.50 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:22:06.52 SS.reconfigure :inhaling 2.14ml/s @16:22:06.60 SS.seek 7ml @16:22:12.66 Stroke completed -- waiting for pressure to equalize ... @16:22:12.68 SS.reconfigure :exhale @16:22:12.77 SS.hold @16:22:16.86 Intake.select :close @16:22:17.07 SSV.dial :exhaust @16:22:17.85 Exhaust.select :open @16:22:17.99 SS.seek 0ml @16:22:22.18 Exhaust.select :close @16:22:22.35 Sampling 1000.0ml @16:22:22.37 Intake.select :close @16:22:22.39 Exhaust.select :close @16:22:22.41 SS.reconfigure :default @16:22:22.49 SS.hold @16:22:22.90 CTV.dial :intake @16:22:22.94 SSV.dial CBV @16:22:22.98
CBV.dial SSV @16:22:23.79 Allowing 130s for 25.0ml of remaining 1000.0ml @16:22:23.81 Intake.select :open @16:22:26.08 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:22:26.12 SS.reconfigure :inhaling 2.14ml/s @16:22:26.20 SS.seek 25ml @16:22:30.89 Vacuum too high -- waiting up to 40 seconds @16:22:30.91 SS.reconfigure :exhale @16:22:30.99 SS.hold @16:22:31.91 Intake @12.0psia, vacuum from 6.5 to 9.5psi @16:22:31.95 SS.reconfigure :inhaling 1.93ml/s @16:22:32.05 SS.seek 25ml @16:22:36.83 Vacuum too high -- waiting up to 40 seconds @16:22:36.85 SS.reconfigure :exhale @16:22:37.01 SS.hold @16:22:38.83 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:22:38.87 SS.reconfigure :inhaling 1.74ml/s @16:22:38.95 SS.seek 25ml @16:22:41.90 Vacuum too high -- waiting up to 40 seconds @16:22:41.92 SS.reconfigure :exhale @16:22:42.02 SS.hold @16:22:42.74 Intake @14.2psia, vacuum from 8.7 to 11.7psi @16:22:42.78 SS.reconfigure :inhaling 1.57ml/s @16:22:42.86 SS.seek 25ml @16:22:45.46 Vacuum too high -- waiting up to 40 seconds @16:22:45.48 SS.reconfigure :exhale @16:22:45.56 SS.hold @16:22:46.52 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:22:46.56 SS.reconfigure :inhaling 1.41ml/s @16:22:46.64 SS.seek 25ml @16:22:50.36 Vacuum too high -- waiting up to 40 seconds @16:22:50.45 SS.reconfigure :exhale @16:22:50.53 SS.hold @16:22:51.85 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:22:51.89 SS.reconfigure :inhaling 1.27ml/s @16:22:51.97 SS.seek 25ml @16:22:55.05 Vacuum too high -- waiting up to 40 seconds @16:22:55.07 SS.reconfigure :exhale @16:22:55.16 SS.hold @16:22:55.76 Intake @14.2psia, vacuum from 8.7 to 11.7psi @16:22:55.80 SS.reconfigure :inhaling 1.14ml/s @16:22:55.88 SS.seek 25ml @16:23:01.60 Stroke completed -- waiting for pressure to equalize ... @16:23:01.62 SS.reconfigure :exhale @16:23:01.70 SS.hold @16:23:02.48 Intake.select :close @16:23:02.69 SSV.dial :exhaust @16:23:03.46 Exhaust.select :open @16:23:03.62 SS.seek 0ml @16:23:12.47 Exhaust.select :close @16:23:12.62 SSV.dial CBV @16:23:12.66
CBV.dial SSV @16:23:13.44 Allowing 130s for 25.0ml of remaining 975.0ml @16:23:13.46 Intake.select :open @16:23:15.70 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:23:15.72 SS.reconfigure :inhaling 1.14ml/s @16:23:15.82 SS.seek 25ml @16:23:17.49 upsync @16:23:24.79
Vacuum too high -- waiting up to 40 seconds @16:23:24.80 SS.reconfigure :exhale @16:23:24.84 SS.hold @16:23:25.84 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:23:25.86 SS.reconfigure :inhaling 1.03ml/s @16:23:25.90 SS.seek 25ml @16:23:37.13 Vacuum too high -- waiting up to 40 seconds @16:23:37.14 SS.reconfigure :exhale @16:23:37.19 SS.hold @16:23:38.46 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:23:38.48 SS.reconfigure :inhaling 0.93ml/s @16:23:38.52 SS.seek 25ml @16:23:47.64 Stroke completed -- waiting for pressure to equalize ... @16:23:47.65 SS.reconfigure :exhale @16:23:47.69 SS.hold @16:23:48.25 Intake.select :close @16:23:48.39 SSV.dial :exhaust @16:23:49.09 Exhaust.select :open @16:23:49.20 SS.seek 0ml @16:23:58.51 Exhaust.select :close @16:23:58.62 SSV.dial CBV @16:23:58.64
CBV.dial SSV @16:23:59.37 Allowing 130s for 25.0ml of remaining 950.0ml @16:23:59.38 Intake.select :open @16:24:01.54 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:24:01.55 SS.reconfigure :inhaling 0.93ml/s @16:24:01.59 SS.seek 25ml @16:24:19.69 Vacuum too high -- waiting up to 40 seconds @16:24:19.70 SS.reconfigure :exhale @16:24:19.74 SS.hold @16:24:20.40 Intake @13.0psia, vacuum from 7.5 to 10.5psi @16:24:20.42 SS.reconfigure :inhaling 0.84ml/s @16:24:20.46 SS.seek 25ml @16:24:33.03 Stroke completed -- waiting for pressure to equalize ... @16:24:33.04 SS.reconfigure :exhale @16:24:33.08 SS.hold @16:24:34.63 Intake.select :close @16:24:34.77 SSV.dial :exhaust @16:24:35.50 Exhaust.select :open @16:24:35.60 SS.seek 0ml @16:24:44.89 Exhaust.select :close @16:24:44.99 SSV.dial CBV @16:24:45.02
CBV.dial SSV @16:24:45.76 Allowing 130s for 25.0ml of remaining 925.0ml @16:24:45.77 Intake.select :open @16:24:47.93 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:24:47.94 SS.reconfigure :inhaling 0.84ml/s @16:24:47.99 SS.seek 25ml @16:25:19.51 Stroke completed -- waiting for pressure to equalize ... @16:25:19.52 SS.reconfigure :exhale @16:25:19.56 SS.hold @16:25:21.19 Intake.select :close @16:25:21.33 SSV.dial :exhaust @16:25:22.06 Exhaust.select :open @16:25:22.17 SS.seek 0ml @16:25:31.40 Exhaust.select :close @16:25:31.50 SSV.dial CBV @16:25:31.53
CBV.dial SSV @16:25:32.26 Allowing 130s for 25.0ml of remaining 900.0ml @16:25:32.27 Intake.select :open @16:25:34.43 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:25:34.44 SS.reconfigure :inhaling 0.84ml/s @16:25:34.49 SS.seek 25ml @16:26:06.07 Stroke completed -- waiting for pressure to equalize ... @16:26:06.08 SS.reconfigure :exhale @16:26:06.12 SS.hold @16:26:08.16 Intake.select :close @16:26:08.30 SSV.dial :exhaust @16:26:09.49 Exhaust.select :open @16:26:09.60 SS.seek 0ml @16:26:18.60 Exhaust.select :close @16:26:18.71 SSV.dial CBV @16:26:18.73
CBV.dial SSV @16:26:19.48 Allowing 130s for 25.0ml of remaining 875.0ml @16:26:19.49 Intake.select :open @16:26:21.64 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:26:21.66 SS.reconfigure :inhaling 0.84ml/s @16:26:21.70 SS.seek 25ml @16:26:54.01 Stroke completed -- waiting for pressure to equalize ... @16:26:54.02 SS.reconfigure :exhale @16:26:54.06 SS.hold @16:26:56.10 Intake.select :close @16:26:56.24 SSV.dial :exhaust @16:26:56.98 Exhaust.select :open @16:26:57.09 SS.seek 0ml @16:27:06.10 Exhaust.select :close @16:27:06.20 SSV.dial CBV @16:27:06.23
CBV.dial SSV @16:27:06.96 Allowing 130s for 25.0ml of remaining 850.0ml @16:27:06.97 Intake.select :open @16:27:09.13 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:27:09.14 SS.reconfigure :inhaling 0.84ml/s @16:27:09.19 SS.seek 25ml @16:27:41.47 Stroke completed -- waiting for pressure to equalize ... @16:27:41.48 SS.reconfigure :exhale @16:27:41.52 SS.hold @16:27:43.47 Intake.select :close @16:27:43.61 SSV.dial :exhaust @16:27:44.32 Exhaust.select :open @16:27:44.43 SS.seek 0ml @16:27:53.29 Exhaust.select :close @16:27:53.39 SSV.dial CBV @16:27:53.43
CBV.dial SSV @16:27:54.13 Allowing 130s for 25.0ml of remaining 825.0ml @16:27:54.14 Intake.select :open @16:27:56.29 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:27:56.31 SS.reconfigure :inhaling 0.84ml/s @16:27:56.35 SS.seek 25ml @16:28:28.63 Stroke completed -- waiting for pressure to equalize ... @16:28:28.64 SS.reconfigure :exhale @16:28:28.68 SS.hold @16:28:31.68 Intake.select :close @16:28:31.82 SSV.dial :exhaust @16:28:32.54 Exhaust.select :open @16:28:32.64 SS.seek 0ml @16:28:41.86 Exhaust.select :close @16:28:41.97 SSV.dial CBV @16:28:41.99
CBV.dial SSV @16:28:42.72 Allowing 130s for 25.0ml of remaining 800.0ml @16:28:42.74 Intake.select :open @16:28:44.89 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:28:44.90 SS.reconfigure :inhaling 0.84ml/s @16:28:44.95 SS.seek 25ml @16:29:16.44 Stroke completed -- waiting for pressure to equalize ... @16:29:16.45 SS.reconfigure :exhale @16:29:16.49 SS.hold @16:29:19.47 Intake.select :close @16:29:19.61 SSV.dial :exhaust @16:29:20.32 Exhaust.select :open @16:29:20.42 SS.seek 0ml @16:29:29.74 Exhaust.select :close @16:29:29.84 SSV.dial CBV @16:29:29.88
CBV.dial SSV @16:29:30.58 Allowing 130s for 25.0ml of remaining 775.0ml @16:29:30.59 Intake.select :open @16:29:32.75 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:29:32.76 SS.reconfigure :inhaling 0.84ml/s @16:29:32.80 SS.seek 25ml @16:29:59.34 Vacuum too high -- waiting up to 40 seconds @16:29:59.35 SS.reconfigure :exhale @16:29:59.39 SS.hold @16:30:00.04 Intake @12.3psia, vacuum from 6.8 to 9.8psi @16:30:00.06 SS.reconfigure :inhaling 0.76ml/s @16:30:00.10 SS.seek 25ml @16:30:06.08 Stroke completed -- waiting for pressure to equalize ... @16:30:06.09 SS.reconfigure :exhale @16:30:06.13 SS.hold @16:30:08.08 Intake.select :close @16:30:08.22 SSV.dial :exhaust @16:30:08.94 Exhaust.select :open @16:30:09.05 SS.seek 0ml @16:30:17.89 Exhaust.select :close @16:30:17.99 SSV.dial CBV @16:30:18.02
CBV.dial SSV @16:30:18.75 Allowing 130s for 25.0ml of remaining 750.0ml @16:30:18.76 Intake.select :open @16:30:20.91 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:30:20.93 SS.reconfigure :inhaling 0.76ml/s @16:30:20.97 SS.seek 25ml @16:30:56.58 Stroke completed -- waiting for pressure to equalize ... @16:30:56.59 SS.reconfigure :exhale @16:30:56.63 SS.hold @16:30:59.11 Intake.select :close @16:30:59.25 SSV.dial :exhaust @16:30:59.97 Exhaust.select :open @16:31:00.07 SS.seek 0ml @16:31:09.20 Exhaust.select :close @16:31:09.31 SSV.dial CBV @16:31:09.33
CBV.dial SSV @16:31:10.06 Allowing 130s for 25.0ml of remaining 725.0ml @16:31:10.07 Intake.select :open @16:31:12.23 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:31:12.24 SS.reconfigure :inhaling 0.76ml/s @16:31:12.29 SS.seek 25ml @16:31:46.97 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @16:31:47.40 SS.hold @16:31:49.81 Intake.select :close @16:31:49.95 SSV.dial :exhaust @16:31:50.67 Exhaust.select :open @16:31:50.78 SS.seek 0ml @16:31:59.75 Exhaust.select :close @16:31:59.85 SSV.dial CBV @16:31:59.90
CBV.dial SSV @16:32:00.61 Allowing 130s for 25.0ml of remaining 700.0ml @16:32:00.62 Intake.select :open @16:32:02.77 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:32:02.79 SS.reconfigure :inhaling 0.76ml/s @16:32:02.83 SS.seek 25ml @16:32:38.37 Stroke completed -- waiting for pressure to equalize ... @16:32:38.38 SS.reconfigure :exhale @16:32:38.42 SS.hold @16:32:41.88 Intake.select :close @16:32:42.02 SSV.dial :exhaust @16:32:42.75 Exhaust.select :open @16:32:42.85 SS.seek 0ml @16:32:51.82 Exhaust.select :close @16:32:51.93 SSV.dial CBV @16:32:51.95
CBV.dial SSV @16:32:52.69 Allowing 130s for 25.0ml of remaining 675.0ml @16:32:52.70 Intake.select :open @16:32:54.85 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:32:54.86 SS.reconfigure :inhaling 0.76ml/s @16:32:54.91 SS.seek 25ml @16:33:30.51 Stroke completed -- waiting for pressure to equalize ... @16:33:30.52 SS.reconfigure :exhale @16:33:30.56 SS.hold @16:33:34.10 Intake.select :close @16:33:34.24 SSV.dial :exhaust @16:33:34.95 Exhaust.select :open @16:33:35.06 SS.seek 0ml @16:33:43.92 Exhaust.select :close @16:33:44.02 SSV.dial CBV @16:33:44.05
CBV.dial SSV @16:33:44.78 Allowing 130s for 25.0ml of remaining 650.0ml @16:33:44.79 Intake.select :open @16:33:46.94 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:33:46.96 SS.reconfigure :inhaling 0.76ml/s @16:33:47.00 SS.seek 25ml @16:34:16.11 Vacuum too high -- waiting up to 40 seconds @16:34:16.12 SS.reconfigure :exhale @16:34:16.16 SS.hold @16:34:17.43 Intake @13.1psia, vacuum from 7.6 to 10.6psi @16:34:17.45 SS.reconfigure :inhaling 0.69ml/s @16:34:17.49 SS.seek 25ml @16:34:25.02 Stroke completed -- waiting for pressure to equalize ... @16:34:25.03 SS.reconfigure :exhale @16:34:25.07 SS.hold @16:34:27.51 Intake.select :close @16:34:27.65 SSV.dial :exhaust @16:34:28.39 Exhaust.select :open @16:34:28.50 SS.seek 0ml @16:34:37.46 Exhaust.select :close @16:34:37.57 SSV.dial CBV @16:34:37.59
CBV.dial SSV @16:34:38.34 Allowing 130s for 25.0ml of remaining 625.0ml @16:34:38.35 Intake.select :open @16:34:40.50 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:34:40.52 SS.reconfigure :inhaling 0.69ml/s @16:34:40.56 SS.seek 25ml @16:35:19.57 Stroke completed -- waiting for pressure to equalize ... @16:35:19.58 SS.reconfigure :exhale @16:35:19.62 SS.hold @16:35:22.61 Intake.select :close @16:35:22.75 SSV.dial :exhaust @16:35:23.48 Exhaust.select :open @16:35:23.58 SS.seek 0ml @16:35:32.86 Exhaust.select :close @16:35:32.97 SSV.dial CBV @16:35:33.00
CBV.dial SSV @16:35:33.73 Allowing 130s for 25.0ml of remaining 600.0ml @16:35:33.74 Intake.select :open @16:35:35.89 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:35:35.90 SS.reconfigure :inhaling 0.69ml/s @16:35:35.95 SS.seek 25ml @16:36:14.25 Stroke completed -- waiting for pressure to equalize ... @16:36:14.26 SS.reconfigure :exhale @16:36:14.30 SS.hold @16:36:17.84 Intake.select :close @16:36:17.98 SSV.dial :exhaust @16:36:18.71 Exhaust.select :open @16:36:18.82 SS.seek 0ml @16:36:28.25 Exhaust.select :close @16:36:28.36 SSV.dial CBV @16:36:28.39
CBV.dial SSV @16:36:29.13 Allowing 130s for 25.0ml of remaining 575.0ml @16:36:29.14 Intake.select :open @16:36:31.30 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:36:31.31 SS.reconfigure :inhaling 0.69ml/s @16:36:31.36 SS.seek 25ml @16:37:09.72 Stroke completed -- waiting for pressure to equalize ... @16:37:09.73 SS.reconfigure :exhale @16:37:09.77 SS.hold @16:37:13.93 Intake.select :close @16:37:14.07 SSV.dial :exhaust @16:37:14.80 Exhaust.select :open @16:37:14.91 SS.seek 0ml @16:37:24.16 Exhaust.select :close @16:37:24.26 SSV.dial CBV @16:37:24.29
CBV.dial SSV @16:37:25.02 Allowing 130s for 25.0ml of remaining 550.0ml @16:37:25.03 Intake.select :open @16:37:27.18 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:37:27.20 SS.reconfigure :inhaling 0.69ml/s @16:37:27.24 SS.seek 25ml @16:37:56.10 Vacuum too high -- waiting up to 40 seconds @16:37:56.11 SS.reconfigure :exhale @16:37:56.15 SS.hold @16:37:57.43 Intake @12.7psia, vacuum from 7.2 to 10.2psi @16:37:57.44 SS.reconfigure :inhaling 0.61ml/s @16:37:57.49 SS.seek 25ml @16:38:08.48 Stroke completed -- waiting for pressure to equalize ... @16:38:08.49 SS.reconfigure :exhale @16:38:08.54 SS.hold @16:38:11.52 Intake.select :close @16:38:11.66 SSV.dial :exhaust @16:38:12.38 Exhaust.select :open @16:38:12.48 SS.seek 0ml @16:38:21.44 Exhaust.select :close @16:38:21.54 SSV.dial CBV @16:38:21.57
CBV.dial SSV @16:38:22.30 Allowing 130s for 25.0ml of remaining 525.0ml @16:38:22.31 Intake.select :open @16:38:24.46 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:38:24.48 SS.reconfigure :inhaling 0.61ml/s @16:38:24.53 SS.seek 25ml @16:39:08.09 Stroke completed -- waiting for pressure to equalize ... @16:39:08.10 SS.reconfigure :exhale @16:39:08.14 SS.hold @16:39:12.21 Intake.select :close @16:39:12.34 SSV.dial :exhaust @16:39:13.06 Exhaust.select :open @16:39:13.18 SS.seek 0ml @16:39:22.36 Exhaust.select :close @16:39:22.46 SSV.dial CBV @16:39:22.50
CBV.dial SSV @16:39:23.22 Allowing 130s for 25.0ml of remaining 500.0ml @16:39:23.23 Intake.select :open @16:39:25.38 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:39:25.40 SS.reconfigure :inhaling 0.61ml/s @16:39:25.44 SS.seek 25ml @16:40:01.57 Vacuum too high -- waiting up to 40 seconds @16:40:01.58 SS.reconfigure :exhale @16:40:01.62 SS.hold @16:40:02.79 Intake @13.0psia, vacuum from 7.5 to 10.5psi @16:40:02.81 SS.reconfigure :inhaling 0.56ml/s @16:40:02.85 SS.seek 25ml @16:40:11.31 Stroke completed -- waiting for pressure to equalize ... @16:40:11.32 SS.reconfigure :exhale @16:40:11.36 SS.hold @16:40:14.25 Intake.select :close @16:40:14.85 SSV.dial :exhaust @16:40:15.56 Exhaust.select :open @16:40:15.68 SS.seek 0ml @16:40:24.59 Exhaust.select :close @16:40:24.70 SSV.dial CBV @16:40:24.72
CBV.dial SSV @16:40:25.45 Allowing 130s for 25.0ml of remaining 475.0ml @16:40:25.46 Intake.select :open @16:40:27.62 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:40:27.63 SS.reconfigure :inhaling 0.56ml/s @16:40:27.67 SS.seek 25ml @16:41:16.24 Stroke completed -- waiting for pressure to equalize ... @16:41:16.25 SS.reconfigure :exhale @16:41:16.30 SS.hold @16:41:20.46 Intake.select :close @16:41:20.60 SSV.dial :exhaust @16:41:21.33 Exhaust.select :open @16:41:21.44 SS.seek 0ml @16:41:30.54 Exhaust.select :close @16:41:30.64 SSV.dial CBV @16:41:30.67
CBV.dial SSV @16:41:31.40 Allowing 130s for 25.0ml of remaining 450.0ml @16:41:31.41 Intake.select :open @16:41:33.57 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:41:33.58 SS.reconfigure :inhaling 0.56ml/s @16:41:33.62 SS.seek 25ml @16:42:22.91 Stroke completed -- waiting for pressure to equalize ... @16:42:22.92 SS.reconfigure :exhale @16:42:22.97 SS.hold @16:42:27.78 Intake.select :close @16:42:27.92 SSV.dial :exhaust @16:42:28.63 Exhaust.select :open @16:42:28.74 SS.seek 0ml @16:42:37.74 Exhaust.select :close @16:42:37.84 SSV.dial CBV @16:42:37.87
CBV.dial SSV @16:42:38.60 Allowing 130s for 25.0ml of remaining 425.0ml @16:42:38.61 Intake.select :open @16:42:40.77 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:42:40.78 SS.reconfigure :inhaling 0.56ml/s @16:42:40.83 SS.seek 25ml @16:43:30.27 Stroke completed -- waiting for pressure to equalize ... @16:43:30.28 SS.reconfigure :exhale @16:43:30.32 SS.hold @16:43:35.96 Intake.select :close @16:43:36.10 SSV.dial :exhaust @16:43:36.82 Exhaust.select :open @16:43:36.93 SS.seek 0ml @16:43:45.97 Exhaust.select :close @16:43:46.08 SSV.dial CBV @16:43:46.10
CBV.dial SSV @16:43:46.84 Allowing 130s for 25.0ml of remaining 400.0ml @16:43:46.85 Intake.select :open @16:43:49.00 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:43:49.01 SS.reconfigure :inhaling 0.56ml/s @16:43:49.06 SS.seek 25ml @16:44:32.09 Vacuum too high -- waiting up to 40 seconds @16:44:32.10 SS.reconfigure :exhale @16:44:32.14 SS.hold @16:44:32.42 Intake @12.4psia, vacuum from 6.9 to 9.9psi @16:44:32.44 SS.reconfigure :inhaling 0.50ml/s @16:44:32.48 SS.seek 25ml @16:44:40.06 Stroke completed -- waiting for pressure to equalize ... @16:44:40.07 SS.reconfigure :exhale @16:44:40.12 SS.hold @16:44:44.18 Intake.select :close @16:44:44.31 SSV.dial :exhaust @16:44:45.02 Exhaust.select :open @16:44:45.13 SS.seek 0ml @16:44:54.58 Exhaust.select :close @16:44:54.68 SSV.dial CBV @16:44:54.71
CBV.dial SSV @16:44:55.44 Allowing 130s for 25.0ml of remaining 375.0ml @16:44:55.45 Intake.select :open @16:44:57.60 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:44:58.06 SS.reconfigure :inhaling 0.50ml/s @16:44:58.12 SS.seek 25ml @16:45:53.87 Stroke completed -- waiting for pressure to equalize ... @16:45:53.88 SS.reconfigure :exhale @16:45:53.93 SS.hold @16:45:58.12 Intake.select :close @16:45:58.26 SSV.dial :exhaust @16:45:58.99 Exhaust.select :open @16:45:59.10 SS.seek 0ml @16:46:08.17 Exhaust.select :close @16:46:08.28 SSV.dial CBV @16:46:08.30
CBV.dial SSV @16:46:09.03 Allowing 130s for 25.0ml of remaining 350.0ml @16:46:09.04 Intake.select :open @16:46:11.20 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:46:11.22 SS.reconfigure :inhaling 0.50ml/s @16:46:11.26 SS.seek 25ml @16:47:08.54 Stroke completed -- waiting for pressure to equalize ... @16:47:08.55 SS.reconfigure :exhale @16:47:08.60 SS.hold @16:47:14.22 Intake.select :close @16:47:14.36 SSV.dial :exhaust @16:47:15.08 Exhaust.select :open @16:47:15.18 SS.seek 0ml @16:47:24.23 Exhaust.select :close @16:47:24.34 SSV.dial CBV @16:47:24.36
CBV.dial SSV @16:47:25.09 Allowing 130s for 25.0ml of remaining 325.0ml @16:47:25.10 Intake.select :open @16:47:27.26 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:47:27.27 SS.reconfigure :inhaling 0.50ml/s @16:47:27.32 SS.seek 25ml @16:48:22.85 Stroke completed -- waiting for pressure to equalize ... @16:48:22.86 SS.reconfigure :exhale @16:48:22.91 SS.hold @16:48:30.27 Intake.select :close @16:48:30.41 SSV.dial :exhaust @16:48:31.12 Exhaust.select :open @16:48:31.23 SS.seek 0ml @16:48:40.64 Exhaust.select :close @16:48:40.74 SSV.dial CBV @16:48:40.77
CBV.dial SSV @16:48:41.50 Allowing 130s for 25.0ml of remaining 300.0ml @16:48:41.51 Intake.select :open @16:48:43.66 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:48:43.68 SS.reconfigure :inhaling 0.50ml/s @16:48:43.72 SS.seek 25ml @16:49:38.43 Stroke completed -- waiting for pressure to equalize ... @16:49:38.44 SS.reconfigure :exhale @16:49:38.48 SS.hold @16:49:45.94 Intake.select :close @16:49:46.08 SSV.dial :exhaust @16:49:46.81 Exhaust.select :open @16:49:46.92 SS.seek 0ml @16:49:56.29 Exhaust.select :close @16:49:56.39 SSV.dial CBV @16:49:56.42
CBV.dial SSV @16:49:57.15 Allowing 130s for 25.0ml of remaining 275.0ml @16:49:57.16 Intake.select :open @16:49:59.31 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:49:59.33 SS.reconfigure :inhaling 0.50ml/s @16:49:59.37 SS.seek 25ml @16:50:43.30 Vacuum too high -- waiting up to 40 seconds @16:50:43.31 SS.reconfigure :exhale @16:50:43.35 SS.hold @16:50:45.74 Intake @13.2psia, vacuum from 7.7 to 10.7psi @16:50:45.76 SS.reconfigure :inhaling 0.46ml/s @16:50:45.80 SS.seek 25ml @16:50:59.32 Stroke completed -- waiting for pressure to equalize ... @16:50:59.33 SS.reconfigure :exhale @16:50:59.37 SS.hold @16:51:04.99 Intake.select :close @16:51:05.13 SSV.dial :exhaust @16:51:05.84 Exhaust.select :open @16:51:05.94 SS.seek 0ml @16:51:14.85 Exhaust.select :close @16:51:14.95 SSV.dial CBV @16:51:14.98
CBV.dial SSV @16:51:15.71 Allowing 130s for 25.0ml of remaining 249.9ml @16:51:15.72 Intake.select :open @16:51:17.87 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:51:17.89 SS.reconfigure :inhaling 0.46ml/s @16:51:17.93 SS.seek 25ml @16:52:20.02 Stroke completed -- waiting for pressure to equalize ... @16:52:20.03 SS.reconfigure :exhale @16:52:20.07 SS.hold @16:52:27.56 Intake.select :close @16:52:27.70 SSV.dial :exhaust @16:52:28.43 Exhaust.select :open @16:52:28.54 SS.seek 0ml @16:52:37.88 Exhaust.select :close @16:52:37.98 SSV.dial CBV @16:52:38.01
CBV.dial SSV @16:52:38.74 Allowing 130s for 25.0ml of remaining 224.9ml @16:52:38.75 Intake.select :open @16:52:40.90 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:52:40.92 SS.reconfigure :inhaling 0.46ml/s @16:52:40.96 SS.seek 25ml @16:53:33.51 Vacuum too high -- waiting up to 40 seconds @16:53:33.52 SS.reconfigure :exhale @16:53:33.56 SS.hold @16:53:37.62 Intake @13.3psia, vacuum from 7.8 to 10.8psi @16:53:37.63 SS.reconfigure :inhaling 0.41ml/s @16:53:37.68 SS.seek 25ml @16:53:46.62 Stroke completed -- waiting for pressure to equalize ... @16:53:46.63 SS.reconfigure :exhale @16:53:46.68 SS.hold @16:53:52.30 Intake.select :close @16:53:52.44 SSV.dial :exhaust @16:53:53.14 Exhaust.select :open @16:53:53.25 SS.seek 0ml @16:54:02.66 Exhaust.select :close @16:54:02.76 SSV.dial CBV @16:54:02.81
CBV.dial SSV @16:54:03.50 Allowing 130s for 25.0ml of remaining 199.9ml @16:54:03.51 Intake.select :open @16:54:05.67 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:54:05.68 SS.reconfigure :inhaling 0.41ml/s @16:54:05.73 SS.seek 25ml @16:55:12.95 Stroke completed -- waiting for pressure to equalize ... @16:55:12.96 SS.reconfigure :exhale @16:55:13.00 SS.hold @16:55:21.60 Intake.select :close @16:55:21.74 SSV.dial :exhaust @16:55:22.47 Exhaust.select :open @16:55:22.58 SS.seek 0ml @16:55:31.64 Exhaust.select :close @16:55:31.74 SSV.dial CBV @16:55:31.77
CBV.dial SSV @16:55:32.50 Allowing 130s for 25.0ml of remaining 174.9ml @16:55:32.51 Intake.select :open @16:55:34.66 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:55:34.68 SS.reconfigure :inhaling 0.41ml/s @16:55:34.72 SS.seek 25ml @16:56:42.76 Stroke completed -- waiting for pressure to equalize ... @16:56:42.77 SS.reconfigure :exhale @16:56:42.81 SS.hold @16:56:52.56 Intake.select :close @16:56:52.70 SSV.dial :exhaust @16:56:53.42 Exhaust.select :open @16:56:53.53 SS.seek 0ml @16:57:02.93 Exhaust.select :close @16:57:03.04 SSV.dial CBV @16:57:03.06
CBV.dial SSV @16:57:03.81 Allowing 130s for 25.0ml of remaining 149.9ml @16:57:03.82 Intake.select :open @16:57:05.97 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:57:05.99 SS.reconfigure :inhaling 0.41ml/s @16:57:06.03 SS.seek 25ml @16:57:57.69 Vacuum too high -- waiting up to 40 seconds @16:57:57.70 SS.reconfigure :exhale @16:57:57.74 SS.hold @16:58:01.31 Intake @13.4psia, vacuum from 7.9 to 10.9psi @16:58:01.32 SS.reconfigure :inhaling 0.37ml/s @16:58:01.37 SS.seek 25ml @16:58:18.93 Stroke completed -- waiting for pressure to equalize ... @16:58:18.94 SS.reconfigure :exhale @16:58:18.98 SS.hold @16:58:27.51 Intake.select :close @16:58:27.65 SSV.dial :exhaust @16:58:28.38 Exhaust.select :open @16:58:28.49 SS.seek 0ml @16:58:37.51 Exhaust.select :close @16:58:37.61 SSV.dial CBV @16:58:37.65
CBV.dial SSV @16:58:38.35 Allowing 130s for 25.0ml of remaining 124.9ml @16:58:38.36 Intake.select :open @16:58:40.52 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:58:40.53 SS.reconfigure :inhaling 0.37ml/s @16:58:40.58 SS.seek 25ml @16:59:56.28 Stroke completed -- waiting for pressure to equalize ... @16:59:56.30 SS.reconfigure :exhale @16:59:56.34 SS.hold @17:00:07.43 Intake.select :close @17:00:07.57 SSV.dial :exhaust @17:00:08.31 Exhaust.select :open @17:00:08.41 SS.seek 0ml @17:00:17.43 Exhaust.select :close @17:00:17.53 SSV.dial CBV @17:00:17.55
CBV.dial SSV @17:00:18.29 Allowing 130s for 25.0ml of remaining 99.9ml @17:00:18.30 Intake.select :open @17:00:20.92 Intake @14.9psia, vacuum from 9.4 to 12.4psi @17:00:20.93 SS.reconfigure :inhaling 0.37ml/s @17:00:20.97 SS.seek 25ml @17:01:37.67 Stroke completed -- waiting for pressure to equalize ... @17:01:37.68 SS.reconfigure :exhale @17:01:37.73 SS.hold @17:01:52.09 Intake.select :close @17:01:52.22 SSV.dial :exhaust @17:01:52.94 Exhaust.select :open @17:01:53.05 SS.seek 0ml @17:02:02.11 Exhaust.select :close @17:02:02.21 SSV.dial CBV @17:02:02.24
CBV.dial SSV @17:02:02.97 Allowing 130s for 25.0ml of remaining 74.9ml @17:02:02.98 Intake.select :open @17:02:05.13 Intake @14.9psia, vacuum from 9.4 to 12.4psi @17:02:05.15 SS.reconfigure :inhaling 0.37ml/s @17:02:05.20 SS.seek 25ml @17:03:08.02 Vacuum too high -- waiting up to 40 seconds @17:03:08.03 SS.reconfigure :exhale @17:03:08.07 SS.hold @17:03:13.69 Intake @14.0psia, vacuum from 8.5 to 11.5psi @17:03:13.71 SS.reconfigure :inhaling 0.33ml/s @17:03:13.75 SS.seek 25ml @17:03:30.34 Stroke completed -- waiting for pressure to equalize ... @17:03:30.35 SS.reconfigure :exhale @17:03:30.39 SS.hold @17:03:41.41 Intake.select :close @17:03:41.55 SSV.dial :exhaust @17:03:42.28 Exhaust.select :open @17:03:42.39 SS.seek 0ml @17:03:51.89 Exhaust.select :close @17:03:51.99 SSV.dial CBV @17:03:52.03
CBV.dial SSV @17:03:52.73 Allowing 130s for 25.0ml of remaining 49.9ml @17:03:52.74 Intake.select :open @17:03:54.90 Intake @15.0psia, vacuum from 9.5 to 12.5psi @17:03:54.91 SS.reconfigure :inhaling 0.33ml/s @17:03:54.96 SS.seek 25ml @17:05:22.23 Stroke completed -- waiting for pressure to equalize ... @17:05:22.24 SS.reconfigure :exhale @17:05:22.28 SS.hold @17:05:34.92 Intake.select :close @17:05:35.06 SSV.dial :exhaust @17:05:35.78 Exhaust.select :open @17:05:35.88 SS.seek 0ml @17:05:45.27 Exhaust.select :close @17:05:45.38 SSV.dial CBV @17:05:45.40
CBV.dial SSV @17:05:46.12 Allowing 130s for 24.9ml of remaining 24.9ml @17:05:46.13 Intake.select :open @17:05:48.28 Intake @15.0psia, vacuum from 9.5 to 12.5psi @17:05:48.30 SS.reconfigure :inhaling 0.33ml/s @17:05:48.34 SS.seek 24.94ml @17:07:16.68 Stroke completed -- waiting for pressure to equalize ... @17:07:16.69 SS.reconfigure :exhale @17:07:16.73 SS.hold @17:07:32.99 Intake.select :close @17:07:33.13 SSV.dial :exhaust @17:07:33.83 Exhaust.select :open @17:07:33.94 SS.seek 0ml @17:07:43.24 Exhaust.select :close @17:07:43.34 Sampled 1000.0ml @17:07:43.35 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:07:43.36 Intake.select :close @17:07:43.42 CSV.dial :waste @17:07:43.49 CS.seek 0ml @17:07:43.56 CSV.dial :air @17:07:43.59 SSV.dial CBV,avoiding: :exhaust @17:07:43.64
CBV.dial SSV @17:07:44.33 CS.seek 10ml @17:07:45.46
CTV.dial :air @17:07:46.19 delay 15 seconds @17:08:01.20 SSV.dial :spit,avoiding: :exhaust @17:08:02.50 CTV.dial CSV,avoiding: :intake @17:08:02.53
SS.seek 0ml @17:08:02.64 SSV.dial CBV,avoiding: :exhaust @17:08:06.98 CBV.dial between SSV and waste2 @17:08:07.35 CTV.dial CSV,avoiding: :air @17:08:07.38
CSV.dial CTV @17:08:07.81 CS.reconfigure :pressurize @17:08:07.86 CS.seek 0ml @17:08:26.51 BubblePoint: Intake @40.3psia, Outlet @16.2psia @17:08:26.52 CBV.dial SSV @17:08:26.84 SS.reconfigure :default @17:08:26.89 SS.seek 10ml @17:08:32.50 delay 15 seconds @17:08:47.62 Evacuated: Intake @16.2psia, Outlet @10.9psia @17:08:47.63 SSV.dial :spit,avoiding: :exhaust @17:08:48.92 SS.reconfigure :exhale @17:08:48.96 SS.seek 0ml @17:08:54.58 SS.reconfigure :fast @17:08:54.62 SS.seek 5ml @17:08:57.26 SSV.dial CBV,avoiding: :exhaust @17:08:57.29
CBV.dial SSV @17:08:58.57 CTV.dial :air,avoiding: :intake @17:08:59.83 CSV.dial CTV @17:08:59.86
SS.seek 10ml @17:09:00.86 CSV.dial :air @17:09:01.31 CS.reconfigure :fast @17:09:01.36 CS.seek 0ml @17:09:02.33
SSV.dial :spit,avoiding: :exhaust @17:09:03.64 SS.reconfigure :exhale @17:09:03.68 SS.seek 0ml @17:09:08.29 CS.stop @17:09:09.29
SS.reconfigure :fast @17:09:09.34 SS.seek 5ml @17:09:12.01 SSV.dial CBV,avoiding: :exhaust @17:09:12.04
CBV.dial SSV @17:09:14.34 SSV.dial :spit,avoiding: :exhaust @17:09:15.62 SS.reconfigure :default @17:09:15.66 SS.seek 0ml @17:09:18.97 SS.stop @17:09:19.01 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @17:09:19.08 CSV.dial :waste @17:09:19.20 SSV.dial CBV,avoiding: :exhaust @17:09:19.23
CBV.dial SSV @17:09:19.83 CS.seek 0ml @17:09:19.89 CSV.dial :air @17:09:20.51
CTV.dial :air @17:09:20.52 delay 15 seconds @17:09:20.72 CS.seek 10ml @17:09:35.54
SSV.dial :spit,avoiding: :exhaust @17:09:36.84 CTV.dial CSV,avoiding: :intake @17:09:36.87
SS.reconfigure :exhale @17:09:36.96 SS.seek 0ml @17:09:37.02 SSV.dial CBV,avoiding: :exhaust @17:09:43.25 CBV.dial between SSV and waste2 @17:09:44.09 CTV.dial CSV,avoiding: :air @17:09:44.11
CSV.dial CTV @17:09:44.56 CS.reconfigure :pressurize @17:09:44.61 CS.seek 0ml @17:10:05.70 BubblePoint: Intake @39.4psia, Outlet @15.4psia @17:10:05.71 CBV.dial SSV @17:10:06.03 SS.reconfigure :default @17:10:06.07 SS.seek 10ml @17:10:11.58 delay 15 seconds @17:10:26.69 Evacuated: Intake @18.3psia, Outlet @12.4psia @17:10:26.71 SSV.dial :spit,avoiding: :exhaust @17:10:27.99 SS.reconfigure :exhale @17:10:28.04 SS.seek 0ml @17:10:33.48 SS.reconfigure :fast @17:10:33.52 SS.seek 5ml @17:10:36.52 SSV.dial CBV,avoiding: :exhaust @17:10:36.55
CBV.dial SSV @17:10:37.83 CTV.dial :air,avoiding: :intake @17:10:39.09 CSV.dial CTV @17:10:39.12
SS.seek 10ml @17:10:40.12 CSV.dial :air @17:10:40.68 CS.reconfigure :fast @17:10:40.74 CS.seek 0ml @17:10:41.94
SSV.dial :spit,avoiding: :exhaust @17:10:43.23 SS.reconfigure :exhale @17:10:43.27 SS.seek 0ml @17:10:45.54 CS.stop @17:10:48.49
SS.reconfigure :fast @17:10:48.54 SS.seek 5ml @17:10:51.36 SSV.dial CBV,avoiding: :exhaust @17:10:51.39
CBV.dial SSV @17:10:53.70 SSV.dial :spit,avoiding: :exhaust @17:10:54.97 SS.reconfigure :default @17:10:55.02 SS.seek 0ml @17:10:58.29 SS.stop @17:10:58.33 Lysing: Adding 2.0ml AqMeOH @17:10:58.34 CBV.dial between CSV and waste1,avoiding: :DBV @17:10:59.78 CTV.dial CSV @17:11:01.04 CSV.dial CSR @17:11:01.78 Pulling 1ml of CSR.AqMeOH @17:11:01.79 CSR.select :AqMeOH @17:11:01.90 CS.reconfigure :default @17:11:01.96 CS.seek 1ml @17:11:05.35 delay 5 seconds @17:11:10.37 CSR.select :air @17:11:10.51 CS.seek 6ml @17:11:23.91 delay 2 seconds @17:11:25.93 CSV.dial :waste2 @17:11:27.00 CS.reconfigure :fast @17:11:27.04 CS.seek 0ml @17:11:40.88 CSV.dial CSR @17:11:41.37 Pulling 2ml of CSR.AqMeOH @17:11:41.38 CSR.select :AqMeOH @17:11:41.49 CS.reconfigure :default @17:11:41.53 CS.seek 2ml @17:11:47.44 delay 5 seconds @17:11:52.46 CSR.select :air @17:11:52.61 CS.seek 5ml @17:12:01.04 delay 2 seconds @17:12:03.05 CSV.dial CTV @17:12:03.48 CS.seek 1ml @17:12:14.55 Before lysing Collection Puck Heater at 19.6C/38.8psia @17:12:14.56 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @17:12:14.58 CH.seek 70C,8:00 @17:13:56.68 CH.hold 10:00,70C @17:13:56.86 Begun lysing Collection Puck Heater at 68C/40.8psia @17:23:56.87 Ended lysing Collection Puck Heater at 70C/42.3psia @17:23:56.89 CS.seek 0ml @17:24:00.40 CTV.dial between spare and air,avoiding: :intake @17:24:01.38 CSV.dial :air @17:24:01.83 CS.seek 5ml @17:24:15.26 CSV.dial CTV @17:24:15.83 delay 2 seconds @17:24:17.84 CSV.dial :air @17:24:18.27 delay 2 seconds @17:24:20.28 CSV.dial CTV @17:24:20.72 delay 2 seconds @17:24:22.73 CSV.dial :air @17:24:23.17 delay 2 seconds @17:24:25.19 CS.seek 0ml @17:24:38.63 Puck @67.1psia after final lysis pressurization @17:24:38.64 Recovering Lysate into Collection syringe @17:24:38.65 CSV.dial CBV @17:24:39.75 CBV.dial CSV @17:24:40.11 CS.seek 5ml @17:24:53.51 delay 5 seconds @17:24:58.53 CTV.dial :air @17:24:59.03 CS.seek 7.001ml @17:25:04.83 delay 5 seconds @17:25:09.84 CSV.dial :air2 @17:25:10.30 CS.seek 10ml @17:25:18.64 CSV.dial between air and CTV,avoiding: CBV @17:25:19.75 Lysing Complete @17:25:19.82 Storing sh1 puck in Collection Clamp to tube 1 @17:25:19.98 SC.reconfigure :tight @17:25:20.02 SC.seek 1 @17:25:23.56 SC.configure :limp @17:25:23.76 Elbow.reconfigure :tight @17:25:23.80 Elbow.seek 1 @17:25:25.59 Forearm.reconfigure :tight @17:25:25.64 Forearm.seek 1 + 700 counts @17:25:26.65 SE.reconfigure :pick @17:25:26.70 SE.seek :beamBreak @17:25:28.73 SE.configure :default @17:25:28.87 Elbow.reconfigure :limp @17:25:28.92 Forearm.reconfigure :loose @17:25:28.96 Forearm.seek 1 @17:25:29.33 Elbow.hold @17:25:29.38 Elbow.reconfigure :tight @17:25:29.48 SE.seek 15.31 Pucks below Top Plate @17:25:29.98 Forearm.force -20 @17:25:30.02 Forearm.seek 1 + 308 counts @17:25:30.40 Forearm.reconfigure :tight @17:25:30.44 Forearm.seek 1 + 700 counts @17:25:30.68 SE.seek 16.31 Pucks below Top Plate @17:25:31.18 Forearm.reconfigure :loose @17:25:31.22 Forearm.seek :retract @17:25:32.14 Elbow.seek CC @17:25:33.81 Elbow.reconfigure :loose @17:25:33.89 Forearm.seek CC @17:25:34.90 Hand.close @17:25:35.67 CC.seek :open @17:25:40.10 Forearm.seek :retract @17:25:41.09 Elbow.reconfigure :tight @17:25:41.14 Elbow.seek 1 @17:25:42.89 Elbow.reconfigure :limp @17:25:42.93 Forearm.seek 1 @17:25:44.08 Elbow.hold @17:25:44.14 Elbow.reconfigure :tight @17:25:44.18 Hand.open @17:25:45.04 Forearm.force -20 @17:25:45.07 Forearm.seek 1 + 318 counts @17:25:45.83 Forearm.reconfigure :tight @17:25:45.87 Forearm.seek 1 + 700 counts @17:25:46.11 SE.seek :down @17:25:47.24
Forearm.reconfigure :loose @17:25:47.28 Forearm.seek :retract @17:25:47.73 SE.stop @17:25:48.27
Tranferring DA @17:25:48.28 Beginning samplePassC @17:25:48.29 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:25:48.47 Elbow.seek FlushPuck::Garage @17:25:50.44 Elbow.reconfigure :loose @17:25:50.53 Forearm.seek :garage @17:25:51.62 Hand.close @17:25:52.28 Forearm.seek :retract @17:25:53.42 Elbow.reconfigure :tight @17:25:53.47 Elbow.seek CC @17:25:56.30 Elbow.reconfigure :loose @17:25:56.39 Forearm.reconfigure :tight @17:25:56.43 Forearm.seek CC @17:25:57.74 CC.seek :closed @17:26:01.98 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.89 @17:26:02.05 Hand.open @17:26:02.85 Forearm.reconfigure :loose @17:26:02.90 Forearm.seek :retract @17:26:03.98 PSV.dial PSR @17:26:05.29 Pulling 1.5ml of PSR.flush PSR.select :flush @17:26:05.41 PS.seek 1.5ml @17:26:21.46 delay 5 seconds @17:26:26.48 PSV.dial :waste1 @17:26:26.91 PS.seek 0ml @17:26:43.01 PSV.dial PSR @17:26:43.58 PSR.select :air @17:26:43.69 PS.seek 2.5ml @17:27:09.91 PRV.dial CBV,avoiding: :ab2 @17:27:09.93
CBV.dial PRV @17:27:11.56 PSV.dial :mixloop @17:27:12.56 CTV.dial :air,avoiding: :intake @17:27:12.57 PTV.dial :PRVmixing @17:27:13.80 PS.seek 0ml @17:27:40.03 PSV.dial :air @17:27:40.45 PS.seek 2.5ml @17:28:06.62 PSV.dial :mixloop @17:28:07.06 PS.seek 0ml @17:28:33.27 PSV.dial :air @17:28:33.72 PS.seek 2.5ml @17:28:59.93 PSV.dial :mixloop @17:29:00.37 PS.seek 0ml @17:29:26.75 Garaging FlushPuck in Collection Clamp @17:29:26.81 Elbow.reconfigure :tight @17:29:26.86 Elbow.seek CC @17:29:26.97 Elbow.reconfigure :loose @17:29:27.06 Forearm.seek CC @17:29:28.07 Hand.close @17:29:28.79 CC.seek :open @17:29:33.12 Forearm.seek :retract @17:29:34.10 Elbow.reconfigure :tight @17:29:34.14 Elbow.seek :garage @17:29:37.00 Elbow.reconfigure :loose @17:29:37.09 Forearm.reconfigure :tight @17:29:37.13 Forearm.seek :garage @17:29:38.23 Hand.open @17:29:38.97 Forearm.reconfigure :loose @17:29:39.01 Forearm.seek :retract @17:29:40.05 PSV.dial :mixloop @17:29:40.06 PTV.dial :PRVmixing @17:29:40.08 PRV.dial CBV @17:29:40.10
CBV.dial PRV @17:29:40.57 CTV.dial :air,avoiding: :intake @17:29:40.59 Loading lyfil puck from tube 2 into Collection Clamp @17:29:40.82 SC.reconfigure :tight @17:29:40.86 SC.seek 2 @17:29:44.45 SC.configure :limp @17:29:44.64 Elbow.reconfigure :tight @17:29:44.69 Elbow.seek 2 @17:29:46.71 Forearm.reconfigure :tight @17:29:46.76 Forearm.seek 2 + 700 counts @17:29:47.79 SE.reconfigure :pick @17:29:47.84 SE.seek :beamBreak @17:29:49.84 SE.configure :default @17:29:50.00 Elbow.seek 2 @17:29:50.32 Forearm.seek 2 + 300 counts @17:29:50.49 Forearm.reconfigure :loose @17:29:50.53 Hand.close @17:29:51.18 SE.seek :down @17:29:51.37
Forearm.seek :retract @17:29:52.43 Elbow.seek CC @17:29:53.04 SE.stop @17:29:54.08
Elbow.reconfigure :loose @17:29:54.17 Forearm.reconfigure :tight @17:29:54.21 Forearm.seek CC @17:29:55.48 CC.seek :closed @17:29:59.71 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.65 @17:29:59.72 Hand.open @17:30:00.47 Forearm.reconfigure :loose @17:30:00.51 Forearm.seek :retract @17:30:01.59 CTV.dial CSV,avoiding: :intake @17:30:02.85 CSV.dial CTV @17:30:03.17 PS.seek 2.5ml @17:30:29.36 CS.seek 5.5ml @17:30:41.54 delay 8 seconds @17:30:49.56 PSV.dial :air @17:30:50.01 delay 2 seconds @17:30:52.02 PSV.dial :waste2 @17:30:52.75 PS.seek 0ml @17:31:18.96 CBV.dial between PRV and waste1 @17:31:19.29 CSV.dial :air @17:31:19.74 CS.seek 10ml @17:31:31.97 CSV.dial CTV @17:31:32.52 CS.seek 0ml @17:31:58.59 CBV.dial :waste1 @17:31:58.88 delay 5 seconds @17:32:03.90 CTV.dial :air,avoiding: :intake @17:32:05.20 Storing lyfil puck in Collection Clamp to tube 1 @17:32:05.36 SC.reconfigure :tight @17:32:05.40 SC.seek 1 @17:32:09.01 SC.configure :limp @17:32:09.21 Elbow.reconfigure :tight @17:32:09.25 Elbow.seek 1 @17:32:11.13 Forearm.reconfigure :tight @17:32:11.17 Forearm.seek 1 + 700 counts @17:32:12.26 SE.reconfigure :pick @17:32:12.32 SE.seek :beamBreak @17:32:14.39 SE.configure :default @17:32:14.53 Elbow.reconfigure :limp @17:32:14.58 Forearm.reconfigure :loose @17:32:14.62 Forearm.seek 1 @17:32:15.03 Elbow.hold @17:32:15.09 Elbow.reconfigure :tight @17:32:15.18 SE.seek 16.33 Pucks below Top Plate @17:32:16.10 Forearm.force -20 @17:32:16.14 Forearm.seek 1 + 316 counts @17:32:16.46 Forearm.reconfigure :tight @17:32:16.51 Forearm.seek 1 + 700 counts @17:32:16.84 SE.seek 17.33 Pucks below Top Plate @17:32:17.44 Forearm.reconfigure :loose @17:32:17.49 Forearm.seek :retract @17:32:18.43 Elbow.seek CC @17:32:20.04 Elbow.reconfigure :loose @17:32:20.13 Forearm.seek CC @17:32:21.06 Hand.close @17:32:21.66 CC.seek :open @17:32:26.09 Forearm.seek :retract @17:32:27.07 Elbow.reconfigure :tight @17:32:27.11 Elbow.seek 1 @17:32:28.91 Elbow.reconfigure :limp @17:32:28.96 Forearm.seek 1 @17:32:30.05 Elbow.hold @17:32:30.11 Elbow.reconfigure :tight @17:32:30.15 Hand.open @17:32:31.03 Forearm.force -20 @17:32:31.06 Forearm.seek 1 + 319 counts @17:32:31.41 Forearm.reconfigure :tight @17:32:31.46 Forearm.seek 1 + 700 counts @17:32:31.75 SE.seek :down @17:32:32.88
Forearm.reconfigure :loose @17:32:32.92 Forearm.seek :retract @17:32:33.35 SE.stop @17:32:33.91
PSV.dial :air @17:32:34.62 PS.seek 2.5ml @17:33:00.86 delay 1 second @17:33:01.88 PSV.dial :mixloop @17:33:02.30 delay 1 second @17:33:03.31 PSV.dial :air @17:33:03.74 delay 1 second @17:33:04.75 PSV.dial :mixloop @17:33:05.18 delay 1 second @17:33:06.19 PSV.dial :air @17:33:06.63 delay 1 second @17:33:07.65 PS.seek 0ml @17:33:33.86 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:33:34.03 Elbow.seek FlushPuck::Garage @17:33:35.83 Elbow.reconfigure :loose @17:33:35.92 Forearm.seek :garage @17:33:37.03 Hand.close @17:33:37.77 Forearm.seek :retract @17:33:38.83 Elbow.reconfigure :tight @17:33:38.88 Elbow.seek PC @17:33:40.19 Elbow.reconfigure :loose @17:33:40.28 Forearm.reconfigure :tight @17:33:40.32 Forearm.seek PC @17:33:41.66 PC.seek :closed @17:33:45.56 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.43 @17:33:45.63 Hand.open @17:33:46.46 Forearm.reconfigure :loose @17:33:46.50 Forearm.seek :retract @17:33:47.51 PRV.dial :air @17:33:47.94 PSV.dial :mixloop @17:33:48.42 PS.seek 0.3ml @17:33:52.33 PSV.dial PSR @17:33:53.35 Pulling 0.4ml of PSR.air @17:33:53.36 PSR.select :air @17:33:53.38 PS.seek 0.7ml @17:33:58.77 delay 3 seconds @17:34:01.78 PSR.select :air @17:34:01.80 PS.seek 2.5ml @17:34:20.86 delay 3 seconds @17:34:23.88 PSV.dial :mixloop @17:34:24.98 PTV.dial :mixing @17:34:25.69 PBV.dial :waste2 @17:34:26.56 PS.seek 0ml @17:34:52.77 Garaging FlushPuck in Processing Clamp @17:34:52.84 Elbow.reconfigure :tight @17:34:52.88 Elbow.seek PC @17:34:52.99 Elbow.reconfigure :loose @17:34:53.08 Forearm.seek PC @17:34:54.17 Hand.close @17:34:54.78 PC.seek :open @17:34:58.75 Forearm.seek :retract @17:34:59.78 Elbow.reconfigure :tight @17:34:59.82 Elbow.seek :garage @17:35:01.16 Elbow.reconfigure :loose @17:35:01.25 Forearm.reconfigure :tight @17:35:01.29 Forearm.seek :garage @17:35:02.40 Hand.open @17:35:03.21 Forearm.reconfigure :loose @17:35:03.25 Forearm.seek :retract @17:35:04.28 Loading da puck from tube 2 into Processing Clamp @17:35:04.50 SC.reconfigure :tight @17:35:04.54 SC.seek 2 @17:35:08.19 SC.configure :limp @17:35:08.38 Elbow.reconfigure :tight @17:35:08.42 Elbow.seek 2 @17:35:10.39 Forearm.reconfigure :tight @17:35:10.44 Forearm.seek 2 + 700 counts @17:35:11.45 SE.reconfigure :pick @17:35:11.50 SE.seek :beamBreak @17:35:13.69 SE.configure :default @17:35:13.83 Elbow.seek 2 @17:35:14.00 Forearm.seek 2 + 300 counts @17:35:14.17 Forearm.reconfigure :loose @17:35:14.21 Hand.close @17:35:14.79 SE.seek :down @17:35:14.98
Forearm.seek :retract @17:35:15.92 Elbow.seek PC @17:35:16.69 SE.stop @17:35:18.61
Elbow.reconfigure :loose @17:35:18.70 Forearm.reconfigure :tight @17:35:18.74 Forearm.seek PC @17:35:20.10 PC.seek :closed @17:35:23.98 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.39 @17:35:23.99 Hand.open @17:35:24.83 Forearm.reconfigure :loose @17:35:24.88 Forearm.seek :retract @17:35:25.91 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @17:35:25.93 PH.seek 28C,5:00 @17:36:16.43 PH.hold 2:00:00,28C @17:36:16.47 delay 1:30 @17:37:46.49 PSR.select :air @17:37:46.99 PSV.dial PSR @17:37:48.02 Pulling 0.5ml of PSR.wash @17:37:48.03 PSR.select :wash @17:37:48.15 PS.seek 0.5ml @17:37:53.98 delay 5 seconds @17:37:59.00 PSR.select :air @17:37:59.11 PS.seek 2ml @17:38:15.13 PTV.dial :puck @17:38:15.84 PSV.dial PTV @17:38:16.54 PBV.dial :waste1 @17:38:17.85 PS.seek 1.5ml @17:38:23.73 delay 3 seconds @17:38:26.74 PBV.dial between PSV and waste1 @17:38:27.10 PS.seek 0ml @17:38:43.14 delay 5:00 @17:43:43.16 recoverPSRreagent08 @17:43:43.17 PTV.dial :puck @17:43:43.18 PBV.dial :waste1 @17:43:43.53 delay 30 seconds @17:44:13.56 PBV.dial between PSV and waste1 @17:44:13.91 PSR.select :air @17:44:13.92 PSV.dial PSR @17:44:14.76 PS.reconfigure :fast @17:44:14.80 PS.seek 2.5ml @17:44:37.53 PSV.dial PTV @17:44:38.22 delay 5 seconds @17:44:43.24 PSV.dial PSR @17:44:43.94 delay 5 seconds @17:44:48.96 PSV.dial PTV @17:44:49.66 delay 5 seconds @17:44:54.67 PSV.dial :air @17:44:55.38 PS.seek 0ml @17:45:17.87 PTV.dial :PRVmixing,via: PRV @17:45:19.15 PSV.dial :mixloop @17:45:19.59 PRV.dial :ab1 @17:45:20.32 PS.reconfigure :slow @17:45:20.36 PS.seek 0.15ml @17:45:23.87 delay 2 seconds @17:45:25.89 CBV.dial PRV @17:45:25.92
PRV.dial CBV @17:45:26.47 PS.seek 0.34ml @17:45:30.87 delay 2 seconds @17:45:32.89 CBV.dial between PRV and PSV @17:45:33.20 PRV.dial :air @17:45:33.64 PS.seek 0.9ml @17:45:45.46 PRV.dial :waste2 @17:45:46.17 PS.seek 0.5ml @17:45:54.84 addPMLReagent08 @17:45:54.85 PSV.dial :air @17:45:55.28 PS.reconfigure :default @17:45:55.33 PS.seek 2ml @17:46:11.43 PSV.dial :PML @17:46:11.85 PTV.dial :mixing @17:46:12.57 PBV.dial :waste1 @17:46:12.98 PS.seek 1.85ml @17:46:15.24 PBV.dial between PSV and waste1 @17:46:15.61 PS.seek 0ml @17:46:35.28 delay 2 seconds @17:46:37.29 Beginning samplePassC @17:46:37.31 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:46:37.47 Elbow.reconfigure :tight @17:46:37.51 Elbow.seek FlushPuck::Garage @17:46:39.01 Elbow.reconfigure :loose @17:46:39.10 Forearm.seek :garage @17:46:40.22 Hand.close @17:46:40.99 Forearm.seek :retract @17:46:42.04 Elbow.reconfigure :tight @17:46:42.08 Elbow.seek CC @17:46:44.95 Elbow.reconfigure :loose @17:46:45.04 Forearm.reconfigure :tight @17:46:45.08 Forearm.seek CC @17:46:46.39 CC.seek :closed @17:46:50.65 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.86 @17:46:50.72 Hand.open @17:46:51.57 Forearm.reconfigure :loose @17:46:51.61 Forearm.seek :retract @17:46:53.08 PSV.dial PSR @17:46:54.11 Pulling 1.5ml of PSR.flush @17:46:54.12 PSR.select :flush @17:46:54.24 PS.seek 1.5ml @17:47:10.31 delay 5 seconds @17:47:15.33 PSV.dial :waste1 @17:47:15.77 PS.seek 0ml @17:47:31.80 PSV.dial PSR @17:47:32.24 PSR.select :air @17:47:32.36 PS.seek 2.5ml @17:47:58.56 PRV.dial CBV,avoiding: :ab2 @17:47:58.59
CBV.dial PRV @17:48:00.13 PSV.dial :mixloop @17:48:01.12 CTV.dial :air,avoiding: :intake @17:48:01.13 PTV.dial :PRVmixing @17:48:01.83 PS.seek 0ml @17:48:28.05 PSV.dial :air @17:48:28.48 PS.seek 2.5ml @17:48:54.61 PSV.dial :mixloop @17:48:55.06 PS.seek 0ml @17:49:21.27 PSV.dial :air @17:49:21.72 PS.seek 2.5ml @17:49:47.93 PSV.dial :mixloop @17:49:48.48 PS.seek 0ml @17:50:14.71 Garaging FlushPuck in Collection Clamp @17:50:14.78 Elbow.reconfigure :tight @17:50:14.82 Elbow.seek CC @17:50:14.93 Elbow.reconfigure :loose @17:50:15.02 Forearm.seek CC @17:50:15.99 Hand.close @17:50:16.62 CC.seek :open @17:50:20.96 Forearm.seek :retract @17:50:21.95 Elbow.reconfigure :tight @17:50:21.99 Elbow.seek :garage @17:50:25.08 Elbow.reconfigure :loose @17:50:25.17 Forearm.reconfigure :tight @17:50:25.22 Forearm.seek :garage @17:50:26.38 Hand.open @17:50:27.18 Forearm.reconfigure :loose @17:50:27.22 Forearm.seek :retract @17:50:28.25 delayUntil "17:54:37.28" @17:50:28.27 Whole Cell Archive sampling 1000ml @17:50:28.29 Loading wcr puck from tube 2 into Collection Clamp @17:50:28.73 Elbow.reconfigure :tight @17:50:28.77 Elbow.seek 2 @17:50:30.89 Forearm.reconfigure :tight @17:50:30.94 Forearm.seek 2 + 700 counts @17:50:32.06 SE.reconfigure :pick @17:50:32.11 SE.seek :beamBreak @17:50:34.38 SE.configure :default @17:50:34.52 Elbow.seek 2 @17:50:34.70 Forearm.seek 2 + 300 counts @17:50:34.95 Forearm.reconfigure :loose @17:50:34.99 Hand.close @17:50:35.63 SE.seek :down @17:50:35.83 Forearm.seek :retract @17:50:36.84 Elbow.seek CC @17:50:37.53 SE.stop @17:50:38.40 Elbow.reconfigure :loose @17:50:38.49 Forearm.reconfigure :tight @17:50:38.54 Forearm.seek CC @17:50:39.75 CC.seek :closed @17:50:43.97 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.73 @17:50:43.98 Hand.open @17:50:44.81 Forearm.reconfigure :loose @17:50:44.85 Forearm.seek :retract @17:50:45.89 collectSample 1000ml w/bubble point of 35psi @17:50:45.90 Priming 7ml @17:50:45.91 Intake.select :close @17:50:45.92 Exhaust.select :close @17:50:45.93 SS.hold @17:50:46.13 Calibrating pressure sensors @17:50:46.14 CTV.dial :air @17:50:46.15 SSV.dial :spit,avoiding: :exhaust @17:50:46.17 SS.reconfigure :fast @17:50:46.22 SS.seek 5ml @17:50:48.97 SSV.dial CBV @17:50:49.00 CBV.dial SSV @17:50:50.30 delay 3.8 seconds @17:50:54.23 SSV.dial :spit @17:50:55.51 SS.seek 0ml @17:50:58.23 SSV.dial CBV @17:50:59.49 CTV.dial :intake @17:51:00.21 SSV.dial CBV @17:51:00.23 CBV.dial SSV @17:51:00.25 Allowing 40s for 7.0ml of remaining 7.0ml @17:51:00.26 Intake.select :open @17:51:02.42 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:51:02.44 SS.reconfigure :inhaling 2.14ml/s @17:51:02.48 SS.seek 7ml @17:51:09.11 Stroke completed -- waiting for pressure to equalize ... @17:51:09.12 SS.reconfigure :exhale @17:51:09.16 SS.hold @17:51:14.71 Intake.select :close @17:51:14.86 SSV.dial :exhaust @17:51:15.59 Exhaust.select :open @17:51:15.70 SS.seek 0ml @17:51:20.11 Exhaust.select :close @17:51:20.21 Sampling 1000.0ml @17:51:20.22 Intake.select :close @17:51:20.23 Exhaust.select :close @17:51:20.25 SS.reconfigure :default @17:51:20.29 SS.hold @17:51:20.48 CTV.dial :intake @17:51:20.50 SSV.dial CBV @17:51:20.52 CBV.dial SSV @17:51:21.27 Allowing 130s for 25.0ml of remaining 1000.0ml @17:51:21.28 Intake.select :open @17:51:23.44 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:51:23.46 SS.reconfigure :inhaling 2.14ml/s @17:51:23.50 SS.seek 25ml @17:51:28.65 Vacuum too high -- waiting up to 40 seconds @17:51:28.66 SS.reconfigure :exhale @17:51:28.70 SS.hold @17:51:30.28 Intake @13.6psia, vacuum from 8.1 to 11.1psi @17:51:30.30 SS.reconfigure :inhaling 1.93ml/s @17:51:30.34 SS.seek 25ml @17:51:33.58 Vacuum too high -- waiting up to 40 seconds @17:51:33.60 SS.reconfigure :exhale @17:51:33.64 SS.hold @17:51:35.57 Intake @13.8psia, vacuum from 8.3 to 11.3psi @17:51:35.59 SS.reconfigure :inhaling 1.74ml/s @17:51:35.64 SS.seek 25ml @17:51:39.58 Vacuum too high -- waiting up to 40 seconds @17:51:39.60 SS.reconfigure :exhale @17:51:39.64 SS.hold @17:51:41.48 Intake @14.2psia, vacuum from 8.7 to 11.7psi @17:51:41.50 SS.reconfigure :inhaling 1.57ml/s @17:51:41.55 SS.seek 25ml @17:51:45.52 Vacuum too high -- waiting up to 40 seconds @17:51:45.53 SS.reconfigure :exhale @17:51:45.58 SS.hold @17:51:46.86 Intake @13.1psia, vacuum from 7.6 to 10.6psi @17:51:46.88 SS.reconfigure :inhaling 1.41ml/s @17:51:46.93 SS.seek 25ml @17:51:51.06 Stroke completed -- waiting for pressure to equalize ... @17:51:51.07 SS.reconfigure :exhale @17:51:51.11 SS.hold @17:51:53.52 Intake.select :close @17:51:53.66 SSV.dial :exhaust @17:51:54.37 Exhaust.select :open @17:51:54.48 SS.seek 0ml @17:52:03.81 Exhaust.select :close @17:52:03.93 SSV.dial CBV @17:52:04.01 CBV.dial SSV @17:52:04.73 Allowing 130s for 25.0ml of remaining 975.0ml @17:52:04.75 Intake.select :open @17:52:06.90 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:52:06.92 SS.reconfigure :inhaling 1.41ml/s @17:52:06.97 SS.seek 25ml @17:52:12.05 Vacuum too high -- waiting up to 40 seconds @17:52:12.07 SS.reconfigure :exhale @17:52:12.11 SS.hold @17:52:12.56 Intake @13.1psia, vacuum from 7.6 to 10.6psi @17:52:12.58 SS.reconfigure :inhaling 1.27ml/s @17:52:12.62 SS.seek 25ml @17:52:18.11 Vacuum too high -- waiting up to 40 seconds @17:52:18.13 SS.reconfigure :exhale @17:52:18.17 SS.hold @17:52:19.46 Intake @13.6psia, vacuum from 8.1 to 11.1psi @17:52:19.48 SS.reconfigure :inhaling 1.14ml/s @17:52:19.53 SS.seek 25ml @17:52:30.77 Vacuum too high -- waiting up to 40 seconds @17:52:30.78 SS.reconfigure :exhale @17:52:30.82 SS.hold @17:52:32.47 Intake @13.2psia, vacuum from 7.7 to 10.7psi @17:52:32.49 SS.reconfigure :inhaling 1.03ml/s @17:52:32.54 SS.seek 25ml @17:52:35.57 Stroke completed -- waiting for pressure to equalize ... @17:52:35.59 SS.reconfigure :exhale @17:52:35.63 SS.hold @17:52:37.21 Intake.select :close @17:52:37.36 SSV.dial :exhaust @17:52:38.09 Exhaust.select :open @17:52:38.20 SS.seek 0ml @17:52:47.28 Exhaust.select :close @17:52:47.38 SSV.dial CBV @17:52:47.41 CBV.dial SSV @17:52:48.15 Allowing 130s for 25.0ml of remaining 950.0ml @17:52:48.17 Intake.select :open @17:52:50.32 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:52:50.34 SS.reconfigure :inhaling 1.03ml/s @17:52:50.38 SS.seek 25ml @17:53:11.36 Vacuum too high -- waiting up to 40 seconds @17:53:11.37 SS.reconfigure :exhale @17:53:11.42 SS.hold @17:53:12.08 Intake @13.0psia, vacuum from 7.5 to 10.5psi @17:53:12.10 SS.reconfigure :inhaling 0.93ml/s @17:53:12.15 SS.seek 25ml @17:53:19.31 Stroke completed -- waiting for pressure to equalize ... @17:53:19.32 SS.reconfigure :exhale @17:53:19.36 SS.hold @17:53:21.41 Intake.select :close @17:53:21.56 SSV.dial :exhaust @17:53:22.29 Exhaust.select :open @17:53:22.40 SS.seek 0ml @17:53:31.31 Exhaust.select :close @17:53:31.41 SSV.dial CBV @17:53:31.44 CBV.dial SSV @17:53:32.18 Allowing 130s for 25.0ml of remaining 925.0ml @17:53:32.20 Intake.select :open @17:53:34.36 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:53:34.83 SS.reconfigure :inhaling 0.93ml/s @17:53:34.89 SS.seek 25ml @17:54:03.91 Stroke completed -- waiting for pressure to equalize ... @17:54:03.93 SS.reconfigure :exhale @17:54:03.97 SS.hold @17:54:06.37 Intake.select :close @17:54:06.52 SSV.dial :exhaust @17:54:07.24 Exhaust.select :open @17:54:07.36 SS.seek 0ml @17:54:16.55 Exhaust.select :close @17:54:16.66 SSV.dial CBV @17:54:16.69 CBV.dial SSV @17:54:17.43 Allowing 130s for 25.0ml of remaining 900.0ml @17:54:17.44 Intake.select :open @17:54:19.60 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:54:19.62 SS.reconfigure :inhaling 0.93ml/s @17:54:19.66 SS.seek 25ml @17:54:37.29 recoverPRVreagent08 @17:54:37.31 PTV.dial :mixing @17:54:38.01 PBV.dial :waste1 @17:54:38.37 delay 30 seconds @17:54:48.46 Stroke completed -- waiting for pressure to equalize ... @17:54:48.47 SS.reconfigure :exhale @17:54:48.51 SS.hold @17:54:51.41 Intake.select :close @17:54:51.56 SSV.dial :exhaust @17:54:52.29 Exhaust.select :open @17:54:52.40 SS.seek 0ml @17:55:01.29 Exhaust.select :close @17:55:01.40 SSV.dial CBV @17:55:01.42 CBV.dial SSV @17:55:02.16 Allowing 130s for 25.0ml of remaining 875.0ml @17:55:02.18 Intake.select :open @17:55:04.33 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:55:04.35 SS.reconfigure :inhaling 0.93ml/s @17:55:04.40 SS.seek 25ml @17:55:08.39 PBV.dial between PSV and waste1 @17:55:08.75 PSV.dial :air @17:55:09.19 PS.reconfigure :fast @17:55:09.24 PS.seek 2.5ml @17:55:31.85 PSV.dial :mixloop @17:55:32.31 delay 2 seconds @17:55:34.02 Stroke completed -- waiting for pressure to equalize ... @17:55:34.03 SS.reconfigure :exhale @17:55:34.07 SS.hold @17:55:34.33 PSV.dial :air @17:55:34.78 delay 2 seconds @17:55:36.79 PSV.dial :mixloop @17:55:37.23 delay 2 seconds @17:55:37.55 Intake.select :close @17:55:37.69 SSV.dial :exhaust @17:55:38.43 Exhaust.select :open @17:55:38.54 SS.seek 0ml @17:55:39.24 PSV.dial :air @17:55:39.71 PS.seek 0ml @17:55:47.61 Exhaust.select :close @17:55:47.72 SSV.dial CBV @17:55:47.75 CBV.dial SSV @17:55:48.49 Allowing 130s for 25.0ml of remaining 850.0ml @17:55:48.50 Intake.select :open @17:55:50.66 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:55:50.68 SS.reconfigure :inhaling 0.93ml/s @17:55:50.72 SS.seek 25ml @17:56:02.42 washCycleDA #1 of 2 @17:56:02.43 PSR.select :air @17:56:02.44 PSV.dial PSR @17:56:03.75 Pulling 0.5ml of PSR.wash @17:56:03.76 PSR.select :wash @17:56:03.87 PS.reconfigure :default @17:56:03.92 PS.seek 0.5ml @17:56:09.89 delay 5 seconds @17:56:11.76 Vacuum too high -- waiting up to 40 seconds @17:56:11.77 SS.reconfigure :exhale @17:56:11.82 SS.hold @17:56:13.46 Intake @14.2psia, vacuum from 8.7 to 11.7psi @17:56:13.88 SS.reconfigure :inhaling 0.84ml/s @17:56:14.00 SS.seek 25ml @17:56:14.90 PSR.select :air @17:56:15.02 PS.seek 2ml @17:56:23.69 Stroke completed -- waiting for pressure to equalize ... @17:56:23.70 SS.reconfigure :exhale @17:56:23.75 SS.hold @17:56:26.15 Intake.select :close @17:56:26.30 SSV.dial :exhaust @17:56:27.04 Exhaust.select :open @17:56:27.15 SS.seek 0ml @17:56:30.98 PTV.dial :puck @17:56:31.68 PSV.dial PTV @17:56:32.39 PBV.dial :waste1 @17:56:32.82 PS.seek 1.5ml @17:56:36.21 Exhaust.select :close @17:56:36.31 SSV.dial CBV @17:56:36.34 CBV.dial SSV @17:56:37.10 Allowing 130s for 25.0ml of remaining 825.0ml @17:56:37.11 Intake.select :open @17:56:38.70 delay 3 seconds @17:56:39.27 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:56:39.28 SS.reconfigure :inhaling 0.84ml/s @17:56:39.33 SS.seek 25ml @17:56:41.72 PBV.dial between PSV and waste1 @17:56:42.10 PS.seek 0ml @17:56:58.32 delay 2:00 @17:57:11.66 Stroke completed -- waiting for pressure to equalize ... @17:57:11.67 SS.reconfigure :exhale @17:57:11.71 SS.hold @17:57:15.16 Intake.select :close @17:57:15.31 SSV.dial :exhaust @17:57:16.03 Exhaust.select :open @17:57:16.15 SS.seek 0ml @17:57:25.25 Exhaust.select :close @17:57:25.36 SSV.dial CBV @17:57:25.39 CBV.dial SSV @17:57:26.13 Allowing 130s for 25.0ml of remaining 800.0ml @17:57:26.14 Intake.select :open @17:57:28.30 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:57:28.32 SS.reconfigure :inhaling 0.84ml/s @17:57:28.36 SS.seek 25ml @17:58:00.70 Stroke completed -- waiting for pressure to equalize ... @17:58:00.71 SS.reconfigure :exhale @17:58:00.76 SS.hold @17:58:04.22 Intake.select :close @17:58:04.36 SSV.dial :exhaust @17:58:05.09 Exhaust.select :open @17:58:05.21 SS.seek 0ml @17:58:14.30 Exhaust.select :close @17:58:14.40 SSV.dial CBV @17:58:14.43 CBV.dial SSV @17:58:15.17 Allowing 130s for 25.0ml of remaining 775.0ml @17:58:15.19 Intake.select :open @17:58:17.34 Intake @15.0psia, vacuum from 9.5 to 12.5psi @17:58:17.36 SS.reconfigure :inhaling 0.84ml/s @17:58:17.40 SS.seek 25ml @17:58:48.48 Vacuum too high -- waiting up to 40 seconds @17:58:48.49 SS.reconfigure :exhale @17:58:48.53 SS.hold @17:58:51.03 Intake @14.3psia, vacuum from 8.8 to 11.8psi @17:58:51.05 SS.reconfigure :inhaling 0.76ml/s @17:58:51.10 SS.seek 25ml @17:58:52.83 Stroke completed -- waiting for pressure to equalize ... @17:58:52.84 SS.reconfigure :exhale @17:58:52.88 SS.hold @17:58:54.93 Intake.select :close @17:58:55.07 SSV.dial :exhaust @17:58:55.78 Exhaust.select :open @17:58:55.89 SS.seek 0ml @17:58:58.34 recoverPSRreagent08 @17:58:58.35 PTV.dial :puck @17:58:58.36 PBV.dial :waste1 @17:58:58.72 delay 30 seconds @17:59:05.42 Exhaust.select :close @17:59:05.53 SSV.dial CBV @17:59:05.56 CBV.dial SSV @17:59:06.30 Allowing 130s for 25.0ml of remaining 750.0ml @17:59:06.31 Intake.select :open @17:59:08.47 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:59:08.49 SS.reconfigure :inhaling 0.76ml/s @17:59:08.53 SS.seek 25ml @17:59:28.74 PBV.dial between PSV and waste1 @17:59:29.10 PSR.select :air @17:59:29.12 PSV.dial PSR @17:59:29.84 PS.reconfigure :fast @17:59:29.88 PS.seek 2.5ml @17:59:44.03 Stroke completed -- waiting for pressure to equalize ... @17:59:44.04 SS.reconfigure :exhale @17:59:44.08 SS.hold @17:59:47.55 Intake.select :close @17:59:47.70 SSV.dial :exhaust @17:59:48.42 Exhaust.select :open @17:59:48.53 SS.seek 0ml @17:59:52.60 PSV.dial PTV @17:59:53.32 delay 5 seconds @17:59:57.93 Exhaust.select :close @17:59:58.04 SSV.dial CBV @17:59:58.07 CBV.dial SSV @17:59:58.33 PSV.dial PSR @17:59:58.82 Allowing 130s for 25.0ml of remaining 725.0ml @17:59:58.83 Intake.select :open @17:59:59.05 delay 5 seconds @18:00:00.98 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:00:01.00 SS.reconfigure :inhaling 0.76ml/s @18:00:01.05 SS.seek 25ml @18:00:04.07 PSV.dial PTV @18:00:04.79 delay 5 seconds @18:00:09.81 PSV.dial :air @18:00:10.51 PS.seek 0ml @18:00:33.22 washCycleDA #2 of 2 @18:00:33.23 PSR.select :air @18:00:33.25 PSV.dial PSR @18:00:34.67 Pulling 0.5ml of PSR.wash @18:00:34.68 PSR.select :wash @18:00:34.80 PS.reconfigure :default @18:00:34.84 PS.seek 0.5ml @18:00:35.88 Stroke completed -- waiting for pressure to equalize ... @18:00:35.90 SS.reconfigure :exhale @18:00:35.94 SS.hold @18:00:40.02 Intake.select :close @18:00:40.16 SSV.dial :exhaust @18:00:40.82 delay 5 seconds @18:00:40.89 Exhaust.select :open @18:00:41.00 SS.seek 0ml @18:00:45.83 PSR.select :air @18:00:45.94 PS.seek 2ml @18:00:50.09 Exhaust.select :close @18:00:50.20 SSV.dial CBV @18:00:50.23 CBV.dial SSV @18:00:50.97 Allowing 130s for 25.0ml of remaining 700.0ml @18:00:50.99 Intake.select :open @18:00:53.14 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:00:53.16 SS.reconfigure :inhaling 0.76ml/s @18:00:53.21 SS.seek 25ml @18:01:02.03 PTV.dial :puck @18:01:02.05 PSV.dial PTV @18:01:02.88 PBV.dial :waste1 @18:01:03.29 PS.seek 1.5ml @18:01:09.16 delay 3 seconds @18:01:12.18 PBV.dial between PSV and waste1 @18:01:12.54 PS.seek 0ml @18:01:25.81 Vacuum too high -- waiting up to 40 seconds @18:01:25.83 SS.reconfigure :exhale @18:01:25.87 SS.hold @18:01:28.18 Intake @14.0psia, vacuum from 8.5 to 11.5psi @18:01:28.20 SS.reconfigure :inhaling 0.69ml/s @18:01:28.25 SS.seek 25ml @18:01:28.57 delay 2:00 @18:01:31.87 Stroke completed -- waiting for pressure to equalize ... @18:01:31.88 SS.reconfigure :exhale @18:01:31.92 SS.hold @18:01:34.85 Intake.select :close @18:01:35.00 SSV.dial :exhaust @18:01:35.73 Exhaust.select :open @18:01:35.84 SS.seek 0ml @18:01:44.93 Exhaust.select :close @18:01:45.04 SSV.dial CBV @18:01:45.08 CBV.dial SSV @18:01:45.79 Allowing 130s for 25.0ml of remaining 675.0ml @18:01:45.81 Intake.select :open @18:01:47.96 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:01:47.98 SS.reconfigure :inhaling 0.69ml/s @18:01:48.03 SS.seek 25ml @18:02:27.77 Stroke completed -- waiting for pressure to equalize ... @18:02:27.78 SS.reconfigure :exhale @18:02:27.83 SS.hold @18:02:31.90 Intake.select :close @18:02:32.05 SSV.dial :exhaust @18:02:32.78 Exhaust.select :open @18:02:32.89 SS.seek 0ml @18:02:41.85 Exhaust.select :close @18:02:41.96 SSV.dial CBV @18:02:41.98 CBV.dial SSV @18:02:42.71 Allowing 130s for 25.0ml of remaining 650.0ml @18:02:42.73 Intake.select :open @18:02:44.88 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:02:44.90 SS.reconfigure :inhaling 0.69ml/s @18:02:44.95 SS.seek 25ml @18:03:15.44 Vacuum too high -- waiting up to 40 seconds @18:03:15.45 SS.reconfigure :exhale @18:03:15.49 SS.hold @18:03:18.00 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:03:18.02 SS.reconfigure :inhaling 0.61ml/s @18:03:18.06 SS.seek 25ml @18:03:28.08 Stroke completed -- waiting for pressure to equalize ... @18:03:28.09 SS.reconfigure :exhale @18:03:28.14 SS.hold @18:03:28.63 recoverPSRreagent08 @18:03:28.64 PTV.dial :puck @18:03:28.66 PBV.dial :waste1 @18:03:29.01 delay 30 seconds @18:03:31.05 Intake.select :close @18:03:31.19 SSV.dial :exhaust @18:03:31.91 Exhaust.select :open @18:03:32.03 SS.seek 0ml @18:03:41.41 Exhaust.select :close @18:03:41.52 SSV.dial CBV @18:03:41.56 CBV.dial SSV @18:03:42.29 Allowing 130s for 25.0ml of remaining 625.0ml @18:03:42.30 Intake.select :open @18:03:44.46 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:03:44.47 SS.reconfigure :inhaling 0.61ml/s @18:03:44.52 SS.seek 25ml @18:03:59.03 PBV.dial between PSV and waste1 @18:03:59.39 PSR.select :air @18:03:59.41 PSV.dial PSR @18:04:00.11 PS.reconfigure :fast @18:04:00.16 PS.seek 2.5ml @18:04:22.68 PSV.dial PTV @18:04:23.40 delay 5 seconds @18:04:27.25 Stroke completed -- waiting for pressure to equalize ... @18:04:27.26 SS.reconfigure :exhale @18:04:27.71 SS.hold @18:04:28.42 PSV.dial PSR @18:04:29.14 delay 5 seconds @18:04:31.84 Intake.select :close @18:04:31.98 SSV.dial :exhaust @18:04:32.71 Exhaust.select :open @18:04:32.82 SS.seek 0ml @18:04:34.15 PSV.dial PTV @18:04:34.98 delay 5 seconds @18:04:40.00 PSV.dial :air @18:04:40.74 PS.seek 0ml @18:04:41.83 Exhaust.select :close @18:04:41.94 SSV.dial CBV @18:04:41.97 CBV.dial SSV @18:04:42.71 Allowing 130s for 25.0ml of remaining 600.0ml @18:04:42.72 Intake.select :open @18:04:44.88 Intake @15.2psia, vacuum from 9.7 to 12.7psi @18:04:44.89 SS.reconfigure :inhaling 0.61ml/s @18:04:44.94 SS.seek 25ml @18:05:03.31 getPMLReagent08 @18:05:03.32 PRV.dial :air @18:05:03.76 delay 5 seconds @18:05:08.78 PTV.dial :PRVmixing @18:05:10.04 delay 5 seconds @18:05:15.06 PRV.dial :ab2 @18:05:15.49 PTV.dial :PRVmixing @18:05:15.51 PSV.dial :mixloop @18:05:15.95 PS.reconfigure :default @18:05:16.00 PS.seek 0.3ml @18:05:19.93 delay 2 seconds @18:05:21.95 PRV.dial :air @18:05:22.46 PS.seek 0.55ml @18:05:25.84 addPMLReagent08 @18:05:25.85 PSV.dial :air @18:05:26.30 PS.seek 2ml @18:05:27.81 Stroke completed -- waiting for pressure to equalize ... @18:05:27.83 SS.reconfigure :exhale @18:05:27.87 SS.hold @18:05:33.44 Intake.select :close @18:05:33.59 SSV.dial :exhaust @18:05:34.31 Exhaust.select :open @18:05:34.43 SS.seek 0ml @18:05:41.80 PSV.dial :PML @18:05:42.26 PTV.dial :mixing @18:05:42.98 PBV.dial :waste1 @18:05:43.38 PS.seek 1.85ml @18:05:43.86 Exhaust.select :close @18:05:43.97 SSV.dial CBV @18:05:43.99 CBV.dial SSV @18:05:44.72 Allowing 130s for 25.0ml of remaining 575.0ml @18:05:44.73 Intake.select :open @18:05:45.68 PBV.dial between PSV and waste1 @18:05:46.05 PS.seek 0ml @18:05:46.89 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:05:46.91 SS.reconfigure :inhaling 0.61ml/s @18:05:46.96 SS.seek 25ml @18:06:05.62 delay 2 seconds @18:06:07.64 delay 8:00 @18:06:29.94 Stroke completed -- waiting for pressure to equalize ... @18:06:29.95 SS.reconfigure :exhale @18:06:29.99 SS.hold @18:06:36.49 Intake.select :close @18:06:36.63 SSV.dial :exhaust @18:06:37.36 Exhaust.select :open @18:06:37.47 SS.seek 0ml @18:06:46.44 Exhaust.select :close @18:06:46.54 SSV.dial CBV @18:06:46.57 CBV.dial SSV @18:06:47.81 Allowing 130s for 25.0ml of remaining 550.0ml @18:06:47.83 Intake.select :open @18:06:49.98 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:06:50.00 SS.reconfigure :inhaling 0.61ml/s @18:06:50.05 SS.seek 25ml @18:07:25.15 Vacuum too high -- waiting up to 40 seconds @18:07:25.16 SS.reconfigure :exhale @18:07:25.21 SS.hold @18:07:28.09 Intake @14.0psia, vacuum from 8.5 to 11.5psi @18:07:28.11 SS.reconfigure :inhaling 0.56ml/s @18:07:28.16 SS.seek 25ml @18:07:38.28 Stroke completed -- waiting for pressure to equalize ... @18:07:38.29 SS.reconfigure :exhale @18:07:38.33 SS.hold @18:07:42.41 Intake.select :close @18:07:42.56 SSV.dial :exhaust @18:07:43.30 Exhaust.select :open @18:07:43.41 SS.seek 0ml @18:07:52.68 Exhaust.select :close @18:07:52.79 SSV.dial CBV @18:07:52.82 CBV.dial SSV @18:07:53.56 Allowing 130s for 25.0ml of remaining 525.0ml @18:07:53.57 Intake.select :open @18:07:55.73 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:07:55.75 SS.reconfigure :inhaling 0.56ml/s @18:07:55.79 SS.seek 25ml @18:08:42.99 Stroke completed -- waiting for pressure to equalize ... @18:08:43.01 SS.reconfigure :exhale @18:08:43.05 SS.hold @18:08:49.46 Intake.select :close @18:08:49.61 SSV.dial :exhaust @18:08:50.34 Exhaust.select :open @18:08:50.45 SS.seek 0ml @18:08:59.79 Exhaust.select :close @18:08:59.90 SSV.dial CBV @18:08:59.94 CBV.dial SSV @18:09:00.65 Allowing 130s for 25.0ml of remaining 500.0ml @18:09:00.67 Intake.select :open @18:09:02.83 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:09:02.84 SS.reconfigure :inhaling 0.56ml/s @18:09:02.89 SS.seek 25ml @18:09:46.36 Vacuum too high -- waiting up to 40 seconds @18:09:46.37 SS.reconfigure :exhale @18:09:46.42 SS.hold @18:09:50.59 Intake @14.6psia, vacuum from 9.1 to 12.1psi @18:09:50.61 SS.reconfigure :inhaling 0.50ml/s @18:09:50.66 SS.seek 25ml @18:09:55.10 Stroke completed -- waiting for pressure to equalize ... @18:09:55.11 SS.reconfigure :exhale @18:09:55.15 SS.hold @18:09:59.33 Intake.select :close @18:09:59.47 SSV.dial :exhaust @18:10:00.21 Exhaust.select :open @18:10:00.33 SS.seek 0ml @18:10:09.66 Exhaust.select :close @18:10:09.77 SSV.dial CBV @18:10:09.80 CBV.dial SSV @18:10:10.56 Allowing 130s for 25.0ml of remaining 475.0ml @18:10:10.57 Intake.select :open @18:10:12.73 Intake @15.2psia, vacuum from 9.7 to 12.7psi @18:10:12.75 SS.reconfigure :inhaling 0.50ml/s @18:10:12.79 SS.seek 25ml @18:11:05.97 Stroke completed -- waiting for pressure to equalize ... @18:11:05.99 SS.reconfigure :exhale @18:11:06.03 SS.hold @18:11:13.40 Intake.select :close @18:11:13.55 SSV.dial :exhaust @18:11:14.27 Exhaust.select :open @18:11:14.39 SS.seek 0ml @18:11:23.65 Exhaust.select :close @18:11:23.75 SSV.dial CBV @18:11:23.78 CBV.dial SSV @18:11:24.53 Allowing 130s for 25.0ml of remaining 450.0ml @18:11:24.54 Intake.select :open @18:11:26.69 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:11:26.71 SS.reconfigure :inhaling 0.50ml/s @18:11:26.76 SS.seek 25ml @18:12:19.91 Stroke completed -- waiting for pressure to equalize ... @18:12:19.92 SS.reconfigure :exhale @18:12:19.96 SS.hold @18:12:28.42 Intake.select :close @18:12:28.57 SSV.dial :exhaust @18:12:29.30 Exhaust.select :open @18:12:29.41 SS.seek 0ml @18:12:38.50 Exhaust.select :close @18:12:38.61 SSV.dial CBV @18:12:38.64 CBV.dial SSV @18:12:39.38 Allowing 130s for 25.0ml of remaining 425.0ml @18:12:39.39 Intake.select :open @18:12:41.55 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:12:41.56 SS.reconfigure :inhaling 0.50ml/s @18:12:41.61 SS.seek 25ml @18:13:17.67 Vacuum too high -- waiting up to 40 seconds @18:13:17.68 SS.reconfigure :exhale @18:13:17.72 SS.hold @18:13:21.94 SS.reconfigure :inhaling 0.46ml/s @18:13:21.99 SS.seek 25ml @18:13:42.59 Stroke completed -- waiting for pressure to equalize ... @18:13:42.61 SS.reconfigure :exhale @18:13:42.65 SS.hold @18:13:50.05 Intake.select :close @18:13:50.20 SSV.dial :exhaust @18:13:51.42 Exhaust.select :open @18:13:51.53 SS.seek 0ml @18:14:00.47 Exhaust.select :close @18:14:00.58 SSV.dial CBV @18:14:00.60 CBV.dial SSV @18:14:01.36 Allowing 130s for 25.0ml of remaining 400.0ml @18:14:01.37 Intake.select :open @18:14:03.54 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:14:03.55 SS.reconfigure :inhaling 0.46ml/s @18:14:03.60 SS.seek 25ml @18:14:07.65 recoverPRVreagent08 @18:14:07.66 PTV.dial :mixing @18:14:07.67 PBV.dial :waste1 @18:14:08.03 delay 30 seconds @18:14:38.05 PBV.dial between PSV and waste1 @18:14:38.40 PSV.dial :air @18:14:38.86 PS.reconfigure :fast @18:14:38.91 PS.seek 2.5ml @18:15:01.79 PSV.dial :mixloop @18:15:02.24 delay 2 seconds @18:15:02.98 Stroke completed -- waiting for pressure to equalize ... @18:15:02.99 SS.reconfigure :exhale @18:15:03.04 SS.hold @18:15:04.26 PSV.dial :air @18:15:04.71 delay 2 seconds @18:15:06.75 PSV.dial :mixloop @18:15:07.33 delay 2 seconds @18:15:09.35 PSV.dial :air @18:15:09.80 PS.seek 0ml @18:15:13.05 Intake.select :close @18:15:13.20 SSV.dial :exhaust @18:15:13.94 Exhaust.select :open @18:15:14.05 SS.seek 0ml @18:15:23.14 Exhaust.select :close @18:15:23.25 SSV.dial CBV @18:15:23.27 CBV.dial SSV @18:15:24.02 Allowing 130s for 25.0ml of remaining 375.0ml @18:15:24.03 Intake.select :open @18:15:26.18 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:15:26.20 SS.reconfigure :inhaling 0.46ml/s @18:15:26.25 SS.seek 25ml @18:15:32.54 washCycleDA #1 of 3 @18:15:32.55 PSR.select :air @18:15:32.57 PSV.dial PSR @18:15:33.86 Pulling 0.5ml of PSR.wash @18:15:33.87 PSR.select :wash @18:15:33.99 PS.reconfigure :default @18:15:34.03 PS.seek 0.5ml @18:15:39.89 delay 5 seconds @18:15:44.90 PSR.select :air @18:15:45.02 PS.seek 2ml @18:16:01.07 PTV.dial :puck @18:16:01.79 PSV.dial PTV @18:16:02.49 PBV.dial :waste1 @18:16:02.91 PS.seek 1.5ml @18:16:05.73 Vacuum too high -- waiting up to 40 seconds @18:16:05.74 SS.reconfigure :exhale @18:16:05.78 SS.hold @18:16:08.85 delay 3 seconds @18:16:10.53 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:16:10.55 SS.reconfigure :inhaling 0.41ml/s @18:16:10.60 SS.seek 25ml @18:16:11.87 PBV.dial between PSV and waste1 @18:16:12.23 PS.seek 0ml @18:16:28.29 delay 2:00 @18:16:34.37 Stroke completed -- waiting for pressure to equalize ... @18:16:34.38 SS.reconfigure :exhale @18:16:34.42 SS.hold @18:16:42.32 Intake.select :close @18:16:42.47 SSV.dial :exhaust @18:16:43.20 Exhaust.select :open @18:16:43.31 SS.seek 0ml @18:16:52.68 Exhaust.select :close @18:16:52.79 SSV.dial CBV @18:16:52.83 CBV.dial SSV @18:16:53.54 Allowing 130s for 25.0ml of remaining 350.0ml @18:16:53.56 Intake.select :open @18:16:55.71 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:16:55.73 SS.reconfigure :inhaling 0.41ml/s @18:16:55.78 SS.seek 25ml @18:18:02.19 Stroke completed -- waiting for pressure to equalize ... @18:18:02.21 SS.reconfigure :exhale @18:18:02.25 SS.hold @18:18:13.29 Intake.select :close @18:18:13.43 SSV.dial :exhaust @18:18:14.15 Exhaust.select :open @18:18:14.26 SS.seek 0ml @18:18:23.55 Exhaust.select :close @18:18:23.66 SSV.dial CBV @18:18:23.69 CBV.dial SSV @18:18:24.43 Allowing 130s for 25.0ml of remaining 325.0ml @18:18:24.44 Intake.select :open @18:18:26.60 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:18:26.62 SS.reconfigure :inhaling 0.41ml/s @18:18:26.67 SS.seek 25ml @18:18:28.31 recoverPSRreagent08 @18:18:28.32 PTV.dial :puck @18:18:28.33 PBV.dial :waste1 @18:18:28.68 delay 30 seconds @18:18:58.71 PBV.dial between PSV and waste1 @18:18:59.07 PSR.select :air @18:18:59.08 PSV.dial PSR @18:18:59.80 PS.reconfigure :fast @18:18:59.85 PS.seek 2.5ml @18:19:07.66 Vacuum too high -- waiting up to 40 seconds @18:19:07.67 SS.reconfigure :exhale @18:19:07.71 SS.hold @18:19:12.58 Intake @15.2psia, vacuum from 9.7 to 12.7psi @18:19:12.60 SS.reconfigure :inhaling 0.37ml/s @18:19:12.65 SS.seek 25ml @18:19:22.37 PSV.dial PTV @18:19:23.10 delay 5 seconds @18:19:28.12 PSV.dial PSR @18:19:28.83 delay 5 seconds @18:19:33.85 PSV.dial PTV @18:19:34.56 delay 5 seconds @18:19:39.58 PSV.dial :air @18:19:40.29 PS.seek 0ml @18:19:42.16 Stroke completed -- waiting for pressure to equalize ... @18:19:42.17 SS.reconfigure :exhale @18:19:42.22 SS.hold @18:19:52.23 Intake.select :close @18:19:52.37 SSV.dial :exhaust @18:19:53.10 Exhaust.select :open @18:19:53.21 SS.seek 0ml @18:20:02.22 Exhaust.select :close @18:20:02.84 washCycleDA #2 of 3 @18:20:02.85 PSR.select :air @18:20:02.88 PSV.dial PSR @18:20:02.92 SSV.dial CBV @18:20:02.95 CBV.dial SSV @18:20:03.69 Allowing 130s for 25.0ml of remaining 300.0ml @18:20:03.71 Intake.select :open @18:20:04.18 Pulling 0.5ml of PSR.wash @18:20:04.19 PSR.select :wash @18:20:04.31 PS.reconfigure :default @18:20:04.35 PS.seek 0.5ml @18:20:05.86 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:20:05.88 SS.reconfigure :inhaling 0.37ml/s @18:20:05.93 SS.seek 25ml @18:20:10.32 delay 5 seconds @18:20:15.34 PSR.select :air @18:20:15.45 PS.seek 2ml @18:20:31.44 PTV.dial :puck @18:20:31.45 PSV.dial PTV @18:20:32.26 PBV.dial :waste1 @18:20:32.68 PS.seek 1.5ml @18:20:38.62 delay 3 seconds @18:20:41.64 PBV.dial between PSV and waste1 @18:20:42.01 PS.seek 0ml @18:20:58.03 delay 2:00 @18:21:22.06 Stroke completed -- waiting for pressure to equalize ... @18:21:22.08 SS.reconfigure :exhale @18:21:22.12 SS.hold @18:21:36.40 Intake.select :close @18:21:36.54 SSV.dial :exhaust @18:21:37.25 Exhaust.select :open @18:21:37.36 SS.seek 0ml @18:21:46.64 Exhaust.select :close @18:21:46.75 SSV.dial CBV @18:21:46.79 CBV.dial SSV @18:21:47.49 Allowing 130s for 25.0ml of remaining 275.0ml @18:21:47.50 Intake.select :open @18:21:49.66 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:21:49.67 SS.reconfigure :inhaling 0.37ml/s @18:21:49.72 SS.seek 25ml @18:22:58.05 recoverPSRreagent08 @18:22:58.06 PTV.dial :puck @18:22:58.07 PBV.dial :waste1 @18:22:58.42 delay 30 seconds @18:23:01.96 Vacuum too high -- waiting up to 40 seconds @18:23:01.97 SS.reconfigure :exhale @18:23:02.02 SS.hold @18:23:10.62 Intake @15.0psia, vacuum from 9.5 to 12.5psi @18:23:10.63 SS.reconfigure :inhaling 0.33ml/s @18:23:10.68 SS.seek 25ml @18:23:16.73 Stroke completed -- waiting for pressure to equalize ... @18:23:16.74 SS.reconfigure :exhale @18:23:16.78 SS.hold @18:23:25.36 Intake.select :close @18:23:25.50 SSV.dial :exhaust @18:23:26.22 Exhaust.select :open @18:23:26.33 SS.seek 0ml @18:23:28.44 PBV.dial between PSV and waste1 @18:23:29.31 PSR.select :air @18:23:29.33 PSV.dial PSR @18:23:30.03 PS.reconfigure :fast @18:23:30.08 PS.seek 2.5ml @18:23:35.65 Exhaust.select :close @18:23:35.76 SSV.dial CBV @18:23:35.79 CBV.dial SSV @18:23:36.53 Allowing 130s for 25.0ml of remaining 250.0ml @18:23:36.54 Intake.select :open @18:23:38.70 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:23:38.72 SS.reconfigure :inhaling 0.33ml/s @18:23:38.76 SS.seek 25ml @18:23:52.77 PSV.dial PTV @18:23:53.50 delay 5 seconds @18:23:58.52 PSV.dial PSR @18:23:59.23 delay 5 seconds @18:24:04.25 PSV.dial PTV @18:24:05.07 delay 5 seconds @18:24:10.09 PSV.dial :air @18:24:10.83 PS.seek 0ml @18:24:33.46 washCycleDA #3 of 3 @18:24:33.47 PSR.select :air @18:24:33.49 PSV.dial PSR @18:24:34.78 Pulling 0.5ml of PSR.wash @18:24:34.79 PSR.select :wash @18:24:34.91 PS.reconfigure :default @18:24:34.95 PS.seek 0.5ml @18:24:41.21 delay 5 seconds @18:24:46.23 PSR.select :air @18:24:46.35 PS.seek 2ml @18:25:02.36 PTV.dial :puck @18:25:02.38 PSV.dial PTV @18:25:03.19 PBV.dial :waste1 @18:25:03.61 PS.seek 1.5ml @18:25:07.24 Stroke completed -- waiting for pressure to equalize ... @18:25:07.26 SS.reconfigure :exhale @18:25:07.30 SS.hold @18:25:09.55 delay 3 seconds @18:25:12.57 PBV.dial between PSV and waste1 @18:25:12.94 PS.seek 0ml @18:25:21.59 Intake.select :close @18:25:21.74 SSV.dial :exhaust @18:25:22.46 Exhaust.select :open @18:25:22.57 SS.seek 0ml @18:25:28.93 delay 2:00 @18:25:31.85 Exhaust.select :close @18:25:31.96 SSV.dial CBV @18:25:32.00 CBV.dial SSV @18:25:32.71 Allowing 130s for 25.0ml of remaining 224.9ml @18:25:32.73 Intake.select :open @18:25:34.89 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:25:34.90 SS.reconfigure :inhaling 0.33ml/s @18:25:34.95 SS.seek 25ml @18:26:53.70 Vacuum too high -- waiting up to 40 seconds @18:26:53.71 SS.reconfigure :exhale @18:26:53.76 SS.hold @18:27:01.24 Intake @14.8psia, vacuum from 9.3 to 12.3psi @18:27:01.25 SS.reconfigure :inhaling 0.30ml/s @18:27:01.30 SS.seek 25ml @18:27:14.19 Stroke completed -- waiting for pressure to equalize ... @18:27:14.20 SS.reconfigure :exhale @18:27:14.24 SS.hold @18:27:25.28 Intake.select :close @18:27:25.42 SSV.dial :exhaust @18:27:26.15 Exhaust.select :open @18:27:26.26 SS.seek 0ml @18:27:28.95 recoverPSRreagent08 @18:27:28.96 PTV.dial :puck @18:27:28.97 PBV.dial :waste1 @18:27:29.32 delay 30 seconds @18:27:35.61 Exhaust.select :close @18:27:35.72 SSV.dial CBV @18:27:35.75 CBV.dial SSV @18:27:36.48 Allowing 130s for 25.0ml of remaining 199.9ml @18:27:36.49 Intake.select :open @18:27:38.64 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:27:38.66 SS.reconfigure :inhaling 0.30ml/s @18:27:38.71 SS.seek 25ml @18:27:59.34 PBV.dial between PSV and waste1 @18:27:59.69 PSR.select :air @18:27:59.71 PSV.dial PSR @18:28:00.41 PS.reconfigure :fast @18:28:00.46 PS.seek 2.5ml @18:28:23.27 PSV.dial PTV @18:28:23.99 delay 5 seconds @18:28:29.01 PSV.dial PSR @18:28:29.72 delay 5 seconds @18:28:34.74 PSV.dial PTV @18:28:35.56 delay 5 seconds @18:28:40.58 PSV.dial :air @18:28:41.32 PS.seek 0ml @18:29:03.76 Gate.power :camera,:ON @18:29:03.78 Mixing DA Substrates @18:29:03.79 PSR.select :air @18:29:03.80 PSV.dial PSR @18:29:05.47 Pulling 0.25ml of PSR.sub1 @18:29:05.53 PSR.select :sub1 @18:29:05.78 PS.reconfigure :default @18:29:05.99 PS.seek 0.25ml @18:29:09.53 delay 5 seconds @18:29:14.60 Pulling 0.25ml of PSR.sub2 @18:29:14.61 PSR.select :sub2 @18:29:14.72 PS.seek 0.5ml @18:29:18.05 delay 5 seconds @18:29:18.34 Stroke completed -- waiting for pressure to equalize ... @18:29:18.36 SS.reconfigure :exhale @18:29:18.40 SS.hold @18:29:23.10 PSR.select :air @18:29:23.22 PS.seek 2ml @18:29:36.65 Intake.select :close @18:29:36.80 SSV.dial :exhaust @18:29:37.53 Exhaust.select :open @18:29:37.64 SS.seek 0ml @18:29:39.29 PTV.dial :puck @18:29:39.31 PSV.dial PTV @18:29:40.03 PBV.dial :waste1 @18:29:40.47 PS.seek 1.5ml @18:29:46.33 delay 3 seconds @18:29:47.00 Exhaust.select :close @18:29:47.11 SSV.dial CBV @18:29:47.15 CBV.dial SSV @18:29:47.88 Allowing 130s for 25.0ml of remaining 174.9ml @18:29:47.89 Intake.select :open @18:29:49.34 PBV.dial between PSV and waste1 @18:29:49.73 PS.seek 0ml @18:29:50.04 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:29:50.06 SS.reconfigure :inhaling 0.30ml/s @18:29:50.11 SS.seek 25ml @18:30:05.79 delay 10 seconds @18:30:15.82 recoverPSRreagent08 @18:30:15.83 PTV.dial :puck @18:30:15.84 PBV.dial :waste1 @18:30:16.19 delay 30 seconds @18:30:46.21 PBV.dial between PSV and waste1 @18:30:46.56 PSR.select :air @18:30:46.58 PSV.dial PSR @18:30:47.30 PS.reconfigure :fast @18:30:47.35 PS.seek 2.5ml @18:30:52.69 Vacuum too high -- waiting up to 40 seconds @18:30:52.71 SS.reconfigure :exhale @18:30:52.75 SS.hold @18:31:00.58 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:31:00.60 SS.reconfigure :inhaling 0.27ml/s @18:31:00.64 SS.seek 25ml @18:31:10.07 PSV.dial PTV @18:31:10.80 delay 5 seconds @18:31:15.81 PSV.dial PSR @18:31:16.53 delay 5 seconds @18:31:21.55 PSV.dial PTV @18:31:22.26 delay 5 seconds @18:31:27.28 PSV.dial :air @18:31:28.00 PS.seek 0ml @18:31:40.01 Stroke completed -- waiting for pressure to equalize ... @18:31:40.02 SS.reconfigure :exhale @18:31:40.07 SS.hold @18:31:50.61 Additional air clear @18:31:50.62 PSR.select :air @18:31:50.64 PSV.dial PSR @18:31:52.07 Pulling 2ml of PSR.air @18:31:52.08 PSR.select :air @18:31:52.10 PS.reconfigure :default @18:31:52.14 PS.seek 2ml @18:31:56.26 Intake.select :close @18:31:56.40 SSV.dial :exhaust @18:31:57.14 Exhaust.select :open @18:31:57.25 SS.seek 0ml @18:32:06.59 Exhaust.select :close @18:32:06.70 SSV.dial CBV @18:32:06.72 CBV.dial SSV @18:32:07.47 Allowing 130s for 25.0ml of remaining 149.9ml @18:32:07.49 Intake.select :open @18:32:09.64 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:32:09.66 SS.reconfigure :inhaling 0.27ml/s @18:32:09.71 SS.seek 25ml @18:32:13.39 delay 5 seconds @18:32:18.40 PTV.dial :puck @18:32:18.42 PSV.dial PTV @18:32:19.15 PBV.dial :waste1 @18:32:19.57 PS.seek 1.5ml @18:32:25.48 delay 3 seconds @18:32:28.50 PBV.dial between PSV and waste1 @18:32:28.86 delay 1 second @18:32:29.88 PSV.dial :waste1 @18:32:30.97 PS.seek 0ml @18:32:47.12 Processing Puck Heater at 28.1C before imaging @18:32:47.13 PH.coast @18:32:47.25 Imaging da puck in Processing Clamp while storing it to tube 1 @18:32:47.42 SC.reconfigure :tight @18:32:47.47 SC.seek 1 @18:32:51.06 SC.configure :limp @18:32:51.26 Elbow.reconfigure :tight @18:32:51.30 Elbow.seek 1 @18:32:52.98 Forearm.reconfigure :tight @18:32:53.02 Forearm.seek 1 + 700 counts @18:32:54.19 SE.reconfigure :pick @18:32:54.24 SE.seek :beamBreak @18:32:56.22 SE.configure :default @18:32:56.36 Elbow.reconfigure :limp @18:32:56.41 Forearm.reconfigure :loose @18:32:56.45 Forearm.seek 1 @18:32:56.83 Elbow.hold @18:32:56.90 Elbow.reconfigure :tight @18:32:56.99 SE.seek 17.33 Pucks below Top Plate @18:32:57.47 Forearm.force -20 @18:32:57.51 Forearm.seek 1 + 319 counts @18:32:58.34 Forearm.reconfigure :tight @18:32:58.47 Forearm.seek 1 + 700 counts @18:32:58.79 SE.seek 18.33 Pucks below Top Plate @18:32:59.48 Forearm.reconfigure :loose @18:32:59.52 Forearm.seek :retract @18:33:00.43 Elbow.seek PC @18:33:02.92 Elbow.reconfigure :loose @18:33:03.02 Forearm.seek PC @18:33:04.13 Hand.close @18:33:04.91 PC.seek :open @18:33:08.93 Forearm.seek :retract @18:33:10.04 Elbow.reconfigure :tight @18:33:10.08 Elbow.seek 1 @18:33:12.82 Forearm.reconfigure :tight @18:33:12.86 Forearm.seek 1 + 700 counts @18:33:13.75 Hand.open @18:33:14.60 SE.seek 17.97 Pucks below Top Plate @18:33:14.88 SE.reconfigure :seal @18:33:14.92 SE.seek 16.87 Pucks below Top Plate @18:33:15.50 Lights, camera, ACTION! @18:33:15.54 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22oct2818h1000ml.tif @18:33:31.60 SX MX5/16 (USB:09): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 12.577 seconds /var/log/esp/da22oct2818h1000ml.tif: TIFF Upload Complete @18:33:31.63 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22oct2818h1000ml15s.tif @18:33:34.97 Waiting >20 seconds for da to relinquish FlushPuck @18:33:47.36 SX MX5/16 (USB:09): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22oct2818h1000ml15s.tif: TIFF Upload Complete @18:33:47.39 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da22oct2818h1000ml25s.tif @18:33:59.37 Stroke completed -- waiting for pressure to equalize ... @18:33:59.38 SS.reconfigure :exhale @18:33:59.43 SS.hold @18:34:13.12 SX MX5/16 (USB:09): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da22oct2818h1000ml25s.tif: TIFF Upload Complete @18:34:13.14 Gate.power :camera,:OFF @18:34:13.19 DAfans.email "12.577 sec auto-exposure",Subject: "da22oct2818h1000ml images!" @18:34:13.45 Elbow.seek 1 @18:34:14.05 Forearm.seek 1 + 700 counts @18:34:14.13 SE.reconfigure :gently @18:34:14.23 SE.seek 17.58 Pucks below Top Plate @18:34:14.94 Elbow.reconfigure :limp @18:34:14.99 Forearm.reconfigure :loose @18:34:15.05 Forearm.seek 1 @18:34:15.47 Elbow.hold @18:34:15.54 Elbow.reconfigure :tight @18:34:15.61 SE.reconfigure :default @18:34:15.65 SE.seek 17.77 Pucks below Top Plate @18:34:16.11 Forearm.force -20 @18:34:16.15 Forearm.seek 1 + 316 counts @18:34:16.47 Forearm.reconfigure :tight @18:34:16.52 Forearm.seek 1 + 700 counts @18:34:16.79 SE.seek :down @18:34:17.50 SS.reconfigure :default @18:34:17.54 SS.hold @18:34:17.67 Intake.select :close @18:34:17.82 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 875.1ml @18:34:17.83 SSV.dial :exhaust @18:34:18.49 SE.stop @18:34:18.74 Forearm.reconfigure :loose @18:34:18.86 Forearm.seek :retract @18:34:19.17 Exhaust.select :open @18:34:19.33 SS.reconfigure :exhale @18:34:19.38 SS.seek 0ml @18:34:19.95 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:34:20.23 Elbow.seek FlushPuck::Garage @18:34:22.14 Elbow.reconfigure :loose @18:34:22.34 Forearm.seek :garage @18:34:23.49 Hand.close @18:34:24.47 Forearm.seek :retract @18:34:25.63 Elbow.reconfigure :tight @18:34:25.71 Elbow.seek PC @18:34:27.22 Elbow.reconfigure :loose @18:34:27.42 Forearm.reconfigure :tight @18:34:27.52 Forearm.seek PC @18:34:28.96 Exhaust.select :close @18:34:29.36 Sampled 875.1ml @18:34:29.44 Drying puck before fixing for 20:00 CBV.dial :waste2b @18:34:29.60 PC.seek :closed @18:34:30.27 delay 1:00 @18:34:33.64 Processing Clamp onSpring/offSpring for 9/24 samples, on/off torque 1.40 @18:34:33.80 Hand.open @18:34:34.69 Forearm.reconfigure :loose @18:34:34.78 Forearm.seek :retract @18:34:35.94 PSR.select :air @18:34:35.98 PSV.dial PSR @18:34:36.56 Pulling 0.5ml of PSR.flush @18:34:36.58 PSR.select :flush @18:34:36.78 PS.seek 0.5ml @18:34:42.86 delay 5 seconds @18:34:47.90 PSR.select :air @18:34:48.06 PS.seek 2.5ml @18:35:09.20 delay 1 second @18:35:10.24 PRV.dial :waste2,avoiding: :ab2 @18:35:12.09 PTV.dial :PRVmixing @18:35:13.35 PSV.dial :mixloop @18:35:14.37 PS.seek :empty @18:35:30.30 CSV.dial :air @18:35:30.75 CS.reconfigure :fast @18:35:30.85 CS.seek 10ml @18:35:40.61 PSR.select :air @18:35:40.65 PSV.dial PSR @18:35:41.78 Pulling 0.5ml of PSR.flush @18:35:41.80 PSR.select :flush @18:35:41.98 PS.seek 0.5ml @18:35:47.88 delay 5 seconds @18:35:52.92 PSR.select :air @18:35:53.16 PS.seek 2.5ml @18:35:53.43 Pumping wcr puck from tube 2 up to 35psi... @18:35:53.47 CBV.dial SSV @18:35:54.87 CBV.dial between CSV and waste2b @18:35:55.88 CSV.dial between air and CTV @18:35:56.28 CS.seek 7.43ml @18:36:02.76 CTV.dial CSV,avoiding: :air @18:36:02.80 CSV.dial CTV @18:36:03.83 CS.reconfigure :pressurize @18:36:03.94 CS.seek 0ml @18:36:14.31 delay 1 second @18:36:15.33 PBV.dial :waste1 @18:36:15.74 PTV.dial :mixing @18:36:16.52 PSV.dial :mixloop @18:36:17.54 PS.seek :empty @18:36:18.69 CBV.dial :waste2b @18:36:19.06 CS.reconfigure :default @18:36:19.14 CS.seek 0ml @18:36:24.43 delay 1:00 @18:36:37.13 upsync @18:36:43.72 PSV.dial PSR @18:36:45.20 PSR.select :flush @18:36:45.32 PS.seek 1ml @18:36:56.25 PSR.select :air @18:36:56.37 PS.seek 2.5ml @18:37:12.47 PSV.dial PTV @18:37:13.19 PTV.dial :puck @18:37:13.89 PBV.dial :waste1 @18:37:13.92 PS.seek 0ml @18:37:24.46 CTV.dial :air,avoiding: :intake @18:37:25.70 Fix #1 of 2 (with RNAlater) for 10:00 @18:37:25.71 CSV.dial CSR @18:37:26.22 Pulling 2ml of CSR.RNAlater @18:37:26.23 CSR.select :RNAlater @18:37:26.34 CS.seek 2ml @18:37:32.21 delay 5 seconds @18:37:37.23 CSR.select :air @18:37:37.34 CS.reconfigure :fast @18:37:37.39 CS.seek 10ml @18:37:40.12 PSV.dial PSR @18:37:40.84 PSR.select :flush @18:37:40.96 PS.seek 0.5ml @18:37:46.90 PSR.select :air @18:37:47.02 PS.seek 2.5ml @18:37:55.59 Pumping wcr puck from tube 2 up to 35psi... @18:37:55.60 CBV.dial SSV @18:37:56.87 CBV.dial between CSV and waste2b @18:37:57.78 CSV.dial between air and CTV @18:37:58.37 CS.seek 7.254ml @18:38:05.15 CTV.dial CSV,avoiding: :air @18:38:05.18 CSV.dial CTV @18:38:06.49 CS.reconfigure :pressurize @18:38:06.54 CS.seek 0ml @18:38:08.09 PSV.dial PTV @18:38:08.92 PBV.dial PSV @18:38:09.46 PS.seek 0ml @18:38:23.35 delay 10:00 @18:38:35.72 PSV.dial PBV @18:38:36.15 PS.seek 2.5ml @18:39:02.31 PSV.dial :waste1 @18:39:03.02 PS.seek 0ml @18:39:29.21 PSV.dial PSR @18:39:29.65 PSR.select :air @18:39:29.68 PS.seek 2.5ml @18:39:55.84 PSV.dial PTV @18:39:56.54 PBV.dial PSV @18:39:56.56 PS.seek 0ml @18:40:22.74 PSV.dial PBV @18:40:23.19 PS.seek 2.5ml @18:40:49.31 PSV.dial :waste1 @18:40:50.02 PS.seek 0ml @18:41:16.21 PSV.dial PSR @18:41:16.65 PSR.select :flush @18:41:16.77 PS.seek 1ml @18:41:27.68 PSR.select :air @18:41:27.79 PS.seek 2.5ml @18:41:43.84 PSV.dial PTV @18:41:44.54 PTV.dial :puck @18:41:44.55 PBV.dial :waste1 @18:41:45.08 PS.seek 0ml @18:42:11.34 PSV.dial PSR @18:42:12.05 PSR.select :flush @18:42:12.17 PS.seek 0.5ml @18:42:18.15 PSR.select :air @18:42:18.26 PS.seek 2.5ml @18:42:39.33 PSV.dial PTV @18:42:40.05 PBV.dial PSV @18:42:40.59 PS.seek 0ml @18:43:06.83 PSV.dial PBV @18:43:07.29 PS.seek 2.5ml @18:43:33.43 PSV.dial :waste1 @18:43:34.14 PS.seek 0ml @18:44:00.30 PSV.dial PSR @18:44:00.74 PSR.select :air @18:44:00.76 PS.seek 2.5ml @18:44:26.89 PSV.dial PTV @18:44:27.60 PBV.dial PSV @18:44:27.62 PS.seek 0ml @18:44:54.24 PSV.dial PBV @18:44:54.70 PS.seek 2.5ml @18:45:20.83 PSV.dial :waste1 @18:45:21.54 PS.seek 0ml @18:45:47.80 Garaging FlushPuck in Processing Clamp @18:45:47.87 Elbow.reconfigure :tight @18:45:47.92 Elbow.seek PC @18:45:48.12 Elbow.reconfigure :loose @18:45:48.21 Forearm.seek PC @18:45:49.30 Hand.close @18:45:49.89 PC.seek :open @18:45:53.86 Forearm.seek :retract @18:45:54.90 Elbow.reconfigure :tight @18:45:54.94 Elbow.seek :garage @18:45:56.34 Elbow.reconfigure :loose @18:45:56.43 Forearm.reconfigure :tight @18:45:56.48 Forearm.seek :garage @18:45:57.61 Hand.open @18:45:58.44 Forearm.reconfigure :loose @18:45:58.48 Forearm.seek :retract @18:48:23.37 CBV.dial :waste2b @18:48:23.69 CS.reconfigure :default @18:48:23.74 CS.seek 0ml @18:48:26.38 delay 1:00 @18:49:26.40 CTV.dial :air,avoiding: :intake @18:49:27.65 Fix #2 of 2 (with RNAlater) for 10:00 @18:49:27.66 CSV.dial CSR @18:49:28.13 Pulling 2ml of CSR.RNAlater @18:49:28.14 CSR.select :RNAlater @18:49:28.26 CS.seek 2ml @18:49:34.13 delay 5 seconds @18:49:39.15 CSR.select :air @18:49:39.26 CS.reconfigure :fast @18:49:39.31 CS.seek 10ml @18:49:57.41 Pumping wcr puck from tube 2 up to 35psi... @18:49:57.42 CBV.dial SSV @18:49:58.67 CBV.dial between CSV and waste2b @18:49:59.59 CSV.dial between air and CTV @18:50:00.18 CS.seek 7.197ml @18:50:07.13 CTV.dial CSV,avoiding: :air @18:50:07.16 CSV.dial CTV @18:50:08.45 CS.reconfigure :pressurize @18:50:08.50 CS.seek 0ml @18:50:25.27 delay 10:00 @19:00:25.29 CBV.dial :waste2b @19:00:25.61 CS.reconfigure :default @19:00:25.66 CS.seek 0ml @19:00:28.26 delay 1:00 @19:01:28.29 CTV.dial :air,avoiding: :intake @19:01:29.59 Storing wcr puck in Collection Clamp to tube 1 @19:01:29.75 SC.reconfigure :tight @19:01:29.80 SC.seek 1 @19:01:29.91 SC.configure :limp @19:01:30.11 Elbow.reconfigure :tight @19:01:30.15 Elbow.seek 1 @19:01:32.00 Forearm.reconfigure :tight @19:01:32.05 Forearm.seek 1 + 700 counts @19:01:33.10 SE.reconfigure :pick @19:01:33.15 SE.seek :beamBreak @19:01:35.11 SE.configure :default @19:01:35.27 Elbow.reconfigure :limp @19:01:35.32 Forearm.reconfigure :loose @19:01:35.36 Forearm.seek 1 @19:01:35.77 Elbow.hold @19:01:35.84 Elbow.reconfigure :tight @19:01:35.93 SE.seek 18.32 Pucks below Top Plate @19:01:36.42 Forearm.force -20 @19:01:36.46 Forearm.seek 1 + 319 counts @19:01:37.28 Forearm.reconfigure :tight @19:01:37.33 Forearm.seek 1 + 700 counts @19:01:37.54 SE.seek 19.32 Pucks below Top Plate @19:01:38.21 Forearm.reconfigure :loose @19:01:38.25 Forearm.seek :retract @19:01:39.23 Elbow.seek CC @19:01:40.84 Elbow.reconfigure :loose @19:01:40.94 Forearm.seek CC @19:01:41.96 Hand.close @19:01:42.73 CC.seek :open @19:01:47.17 Forearm.seek :retract @19:01:48.24 Elbow.reconfigure :tight @19:01:48.29 Elbow.seek 1 @19:01:50.12 Elbow.reconfigure :limp @19:01:50.17 Forearm.seek 1 @19:01:51.27 Elbow.hold @19:01:51.34 Elbow.reconfigure :tight @19:01:51.38 Hand.open @19:01:52.22 Forearm.force -20 @19:01:52.26 Forearm.seek 1 + 320 counts @19:01:52.59 Forearm.reconfigure :tight @19:01:52.64 Forearm.seek 1 + 700 counts @19:01:53.02 SE.seek :down @19:01:54.15 Forearm.reconfigure :loose @19:01:54.20 Forearm.seek :retract @19:01:54.50 SE.stop @19:01:55.18
Starting finalPurge @19:01:55.19 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:01:55.37 Elbow.seek FlushPuck::Garage @19:01:57.09 Elbow.reconfigure :loose @19:01:57.19 Forearm.seek :garage @19:01:58.27 Hand.close @19:01:58.96 Forearm.seek :retract @19:01:59.99 Elbow.reconfigure :tight @19:02:00.03 Elbow.seek CC @19:02:02.90 Elbow.reconfigure :loose @19:02:02.99 Forearm.reconfigure :tight @19:02:03.03 Forearm.seek CC @19:02:04.27 CC.seek :closed @19:02:08.50 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.87 @19:02:08.56 Hand.open @19:02:09.41 Forearm.reconfigure :loose @19:02:09.45 Forearm.seek :retract @19:02:10.63 Flushing the Collection Valve::Manifold @19:02:10.75 CSV.dial CSR @19:02:11.22 Pulling 5ml of CSR.flush @19:02:11.23 CSR.select :flush @19:02:11.34 CS.seek 5ml @19:02:24.79 delay 5 seconds @19:02:29.81 CSR.select :air @19:02:29.96 CS.reconfigure :fast @19:02:30.00 CS.seek 7.5ml @19:02:36.32 CSV.dial :waste @19:02:37.56 CS.reconfigure :default @19:02:37.60 CS.seek 0ml @19:02:57.35 Flushing lower manifold's waste1 port @19:02:57.47 CSV.dial :flush @19:02:57.90 CS.reconfigure :fast @19:02:57.95 CS.seek 5ml @19:03:09.66 delay 5 seconds @19:03:14.68 CSV.dial :air @19:03:15.65 CS.seek 7.001ml @19:03:20.92 CSV.dial CTV @19:03:20.94
CTV.dial CSV @19:03:22.24 CBV.dial :waste1 @19:03:22.98 CS.seek 0ml @19:03:38.76 Flushing lower manifold's waste2 port @19:03:38.88 CSV.dial :flush @19:03:39.59 CS.seek 5ml @19:03:51.26 delay 5 seconds @19:03:56.28 CSV.dial :air @19:03:56.90 CS.seek 7ml @19:04:02.10 CSV.dial CTV @19:04:02.13
CTV.dial CSV @19:04:02.70 CBV.dial :waste2 @19:04:03.70 CS.seek 0ml @19:04:19.48 Flushing lower manifold's waste2b port @19:04:19.59 CSV.dial :flush @19:04:20.28 CS.seek 5ml @19:04:31.88 delay 5 seconds @19:04:36.90 CSV.dial :air @19:04:37.40 CS.seek 7ml @19:04:42.55 CSV.dial CTV @19:04:42.57
CTV.dial CSV @19:04:42.99 CBV.dial :waste2b @19:04:43.98 CS.seek 0ml @19:04:59.79 Disinfecting external Intake with kill @19:04:59.90 CSV.dial CSR @19:05:00.44 Pulling 8ml of CSR.kill @19:05:00.45 CSR.select :kill @19:05:00.56 CS.seek 8ml @19:05:18.54 delay 5 seconds @19:05:23.56 CSR.select :air @19:05:23.67 CS.seek 10ml @19:05:28.95 CSV.dial CBV @19:05:28.97
CBV.dial CSV @19:05:29.99 CTV.dial :intake @19:05:30.71 Intake.select :open @19:05:30.81 CS.seek 2ml @19:05:48.63 Intake.select :close @19:05:48.73 CTV.dial :air @19:05:49.45 CS.reconfigure :default @19:05:49.49 CS.seek 0ml @19:05:55.35 CSV.dial CSR @19:05:56.38 Pulling 5ml of CSR.flush @19:05:56.39 CSR.select :flush @19:05:56.51 CS.seek 5ml @19:06:10.01 delay 5 seconds @19:06:15.02 CSR.select :air @19:06:15.13 CS.seek 10ml @19:06:28.59 CSV.dial CBV @19:06:29.57 CS.seek 0ml @19:06:55.63 Garaging FlushPuck in Collection Clamp @19:06:55.70 Elbow.reconfigure :tight @19:06:55.74 Elbow.seek CC @19:06:55.85 Elbow.reconfigure :loose @19:06:55.93 Forearm.seek CC @19:06:56.95 Hand.close @19:06:57.72 CC.seek :open @19:07:02.05 Forearm.seek :retract @19:07:03.11 Elbow.reconfigure :tight @19:07:03.15 Elbow.seek :garage @19:07:06.19 Elbow.reconfigure :loose @19:07:06.28 Forearm.reconfigure :tight @19:07:06.32 Forearm.seek :garage @19:07:07.47 Hand.open @19:07:08.27 Forearm.reconfigure :loose @19:07:08.31 Forearm.seek :retract @19:07:09.32 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 16:17:05 @19:07:09.37 Asleep after completing DA_WCR_102822 assays @19:07:09.39 GoodNews.email "{1 => 20,\n 2 => 13,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:06:21, 24.8C, 23% humidity, 15.8psia, 14.026V, 0.361A, 0.361A avg, 5.07W\nSampled 875.1ml",Subject: "Asleep after completing DA_WCR_102822 assays" @19:07:44.56
Uploading Logs @19:09:01.70 upsync @22:16:10.28UTC09-Nov-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @22:16:12.82 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @22:16:16.70 Polling Can every 10:00 @22:16:16.73 Cleanly exited at 13:39:03UTC04-Nov-22 @22:16:16.77 Loading /home/esp/esp2/mission/DA_WCR_11922.rb mission @22:16:16.82 Preparing to sleep until + 00:00:05 @22:16:16.84 GoodNews.email "Starting DA_WCR_11922 w/pucks in tube 2\n{1 => 20,\n 2 => 13,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:16:16, 22.8C, 26% humidity, 15.7psia, 14.065V, 0.202A, 0.202A avg, 2.84W",Subject: "DA_WCR_11922 begun" @22:16:16.90
Asleep til + 00:00:05 @22:16:16.91 Continuing at 22:16 @22:16:17.18 Gate.power :core,:OFF @22:16:17.19 Gate.power :raw,:OFF @22:16:17.20 Shutdown @22:16:21.06 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_11922.rb:14\n{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:16:16, 22.8C, 26% humidity, 15.7psia, 14.065V, 0.202A, 0.202A avg, 2.84W",Subject: "Asleep until 22:16" @22:16:21.80
Powering core bus @22:16:27.49 Gate.power :raw,:ON @22:16:28.81 CS.configure :default @22:16:28.99 PS.configure :default @22:16:29.23 SS.configure :default @22:16:29.41 CC.configure :default @22:16:29.59 PC.configure :default @22:16:29.82 Forearm.configure :loose @22:16:30.00 Elbow.configure :loose @22:16:30.20 SC.configure :loose @22:16:30.39 SE.configure :default @22:16:30.47 CH.coast @22:16:30.57 CH.configure :default @22:16:30.63 PH.coast @22:16:30.82 PH.configure :default @22:16:30.90 All dwarf controllers running firmware version 3.85 @22:16:31.04 Valve.reset @22:16:31.57 PSV.dial :air @22:16:31.65 Intake.select :close @22:16:31.71 Exhaust.select :close @22:16:31.73 CTV.dial :air @22:16:31.87 CSV.dial :waste1 @22:16:32.14
Forearm.reconfigure :loose @22:16:32.22 Forearm.home.seek :retract @22:16:32.44 SSV.dial :spit,avoiding: :exhaust @22:16:32.71 PS.reconfigure :default @22:16:32.80 PS.home.seek 0ml @22:16:33.02 Hand.close @22:16:33.08
Elbow.jog 1500 @22:16:33.44 CS.reconfigure :fast @22:16:33.51 SS.reconfigure :default @22:16:33.71 CS.home.seek 0ml @22:16:33.82 SS.home.seek 0ml @22:16:34.08
Elbow.reconfigure :loose @22:16:34.16 Elbow.home.seek :clear @22:16:38.75 SE.reconfigure :default @22:16:38.84 SE.home.seek :down @22:16:39.59 SC.reconfigure :tight @22:16:39.71 SC.home.seek :initial @22:16:41.43 PS.stop @22:17:09.83 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @22:17:09.86
Retry #1 of 3 @22:17:10.01 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @22:17:14.33 SC.configure :limp @22:17:14.41 Forearm.seek :retract @22:17:14.55 Elbow.seek :hammer @22:17:15.74 Forearm.reconfigure :tight @22:17:15.82 Forearm.seek retracted @22:17:16.45 CC.reconfigure :default @22:17:16.47 PC.reconfigure :default @22:17:16.63
CC.home.seek :open @22:17:16.67 PC.home.seek :open @22:17:20.52
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @22:17:20.54 Notice.email "At 22:17:20 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_11922.rb:14\n{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:17:20, 22.8C, 26% humidity, 15.7psia, 14.031V, 0.275A, 0.275A avg, 3.86W",Subject: "Started DA_WCR_11922 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @22:17:20.61
Starting initialPurge @22:17:20.64 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:17:20.76 Hand.open @22:17:21.83 Elbow.reconfigure :tight @22:17:21.89 Elbow.seek FlushPuck::Garage @22:17:23.97 Elbow.reconfigure :loose @22:17:24.15 Forearm.reconfigure :loose @22:17:24.23 Forearm.seek :garage @22:17:25.39 Hand.close @22:17:26.27 Forearm.seek :retract @22:17:27.36 Elbow.reconfigure :tight @22:17:27.44 Elbow.seek CC @22:17:30.40 Elbow.reconfigure :loose @22:17:30.58 Forearm.reconfigure :tight @22:17:30.65 Forearm.seek CC @22:17:32.18 CC.seek :closed @22:17:36.56 Collection Clamp onSpring/offSpring for 8/25 samples, on/off torque 1.90 @22:17:36.74 Hand.open @22:17:37.72 Forearm.reconfigure :loose @22:17:37.80 Forearm.seek :retract @22:17:38.84 Flushing the Collection Valve::Manifold @22:17:39.08 CSV.dial CSR @22:17:40.48 Pulling 5ml of CSR.flush @22:17:40.50 CSR.select :flush @22:17:40.66 CS.reconfigure :default @22:17:40.74 CS.seek 5ml @22:17:54.27 delay 5 seconds @22:17:59.28 CSR.select :air @22:17:59.50 CS.reconfigure :fast @22:17:59.58 CS.seek 7.5ml @22:18:05.94 CSV.dial :waste @22:18:07.24 CS.reconfigure :default @22:18:07.32 CS.seek 0ml @22:18:18.90 upsync @22:18:27.02
Purging external Intake @22:18:27.13 CSV.dial :flush @22:18:27.57 CS.reconfigure :fast @22:18:27.61 CS.seek 10ml @22:18:50.11 delay 5 seconds @22:18:55.13 CSV.dial CBV @22:18:55.15
CBV.dial CSV @22:18:55.87 CTV.dial :intake @22:18:56.59 Intake.select :open @22:18:56.70 CS.seek 0ml @22:19:19.04 Intake.select :close @22:19:19.14 CTV.dial :air @22:19:19.88 Flushing upper manifold @22:19:19.99 CSV.dial :flush @22:19:20.71 CS.seek 5ml @22:19:32.32 delay 5 seconds @22:19:37.34 CSV.dial :air @22:19:37.84 CS.seek 7ml @22:19:43.11 CSV.dial CBV @22:19:43.13
CBV.dial CSV @22:19:44.14 CTV.dial :air @22:19:44.16 CS.seek 0ml @22:20:00.11 delay 7 seconds @22:20:07.13 CS.seek 3ml @22:20:14.54 CSV.dial :waste @22:20:14.99 CS.seek 0ml @22:20:22.42 CSV.dial :flush @22:20:22.97 CS.seek 3ml @22:20:30.35 delay 5 seconds @22:20:35.37 CSV.dial :air @22:20:35.89 CS.seek 5ml @22:20:41.10 CSV.dial :air @22:20:41.12 CS.seek 0ml @22:20:52.82 delay 7 seconds @22:20:59.85 CS.seek 3ml @22:21:07.23 CSV.dial :waste @22:21:07.94 CS.seek 0ml @22:21:15.36 Garaging FlushPuck in Collection Clamp @22:21:15.42 Elbow.reconfigure :tight @22:21:15.47 Elbow.seek CC @22:21:15.57 Elbow.reconfigure :loose @22:21:15.66 Forearm.seek CC @22:21:16.60 Hand.close @22:21:17.26 CC.seek :open @22:21:21.56 Forearm.seek :retract @22:21:22.55 Elbow.reconfigure :tight @22:21:22.59 Elbow.seek :garage @22:21:25.65 Elbow.reconfigure :loose @22:21:25.74 Forearm.reconfigure :tight @22:21:25.78 Forearm.seek :garage @22:21:26.86 Hand.open @22:21:27.69 Forearm.reconfigure :loose @22:21:28.14 Forearm.seek :retract @22:21:29.21 da sampling at most 1000ml, wcr at most 1000ml @22:21:29.24 DAfans.email "Started da in tube 2 at 22:21:29UTC09-Nov-22\n{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:17:20, 22.8C, 26% humidity, 15.7psia, 14.031V, 0.275A, 0.275A avg, 3.86W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @22:21:29.26
Loading sh1 puck from tube 2 into Collection Clamp @22:21:29.51 SC.reconfigure :tight @22:21:29.55 SC.seek 2 @22:21:42.58 SC.configure :limp @22:21:43.10 Elbow.reconfigure :tight @22:21:43.18 Elbow.seek 2 @22:21:45.36 Forearm.reconfigure :tight @22:21:45.44 Forearm.seek 2 + 700 counts @22:21:46.61 SE.reconfigure :pick @22:21:46.71 SE.seek :beamBreak @22:21:49.12 SE.configure :default @22:21:49.24 SE.seek :down @22:21:51.25 SE.stop @22:21:51.50 Forearm.reconfigure :loose @22:21:51.58 Forearm.seek :retract @22:21:52.57 Puck::Error in MAIN -- 13 pucks loaded in tube 2 when expecting 9 @22:21:52.59 Waiting to Resume from Checkpoint "Loading sh1 puck from tube 2 into Collection Clamp" @22:21:52.61 BadNews.email "13 pucks loaded in tube 2 when expecting 9",Subject: "Puck::Error in MAIN" @22:23:22.57 upsync @22:30:18.36 Session Begun @22:30:35.78 -> abort! 'mission file incorrect' @22:30:35.79 CmdServer::Client::Error in dave -- Aborting Mission: mission file incorrect @22:30:35.84 BadNews.email "mission file incorrect",Subject: "DA_WCR_11922 ABORTED!" @22:31:09.71
Uploading Logs @22:31:45.69 -> exit @22:31:45.73 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @22:32:33.34 upsync @22:33:24.60UTC09-Nov-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @22:33:27.17 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @22:33:31.08 Polling Can every 10:00 @22:33:31.13 Loading /home/esp/esp2/mission/DA_WCR_11922.rb mission @22:33:31.18 GoodNews.email "Starting DA_WCR_11922 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@22:33:31, 22.9C, 26% humidity, 15.7psia, 14.050V, 0.257A, 0.263A avg, 3.61W",Subject: "DA_WCR_11922 begun" @22:33:31.20
Preparing to sleep until + 00:00:05 @22:33:31.25 Asleep til + 00:00:05 @22:33:31.27 Continuing at 22:33 @22:33:31.56 Gate.power :core,:OFF @22:33:31.57 Gate.power :raw,:OFF @22:33:31.59 Shutdown @22:33:34.97 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_11922.rb:14\n{1 => 20,\n 2 => 13,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:33:31, 22.9C, 26% humidity, 15.7psia, 14.050V, 0.257A, 0.263A avg, 3.61W",Subject: "Asleep until 22:33" @22:33:36.15
Powering core bus @22:33:41.92 Gate.power :raw,:ON @22:33:43.48 CS.configure :default @22:33:43.66 PS.configure :default @22:33:43.84 SS.configure :default @22:33:44.02 CC.configure :default @22:33:44.20 PC.configure :default @22:33:44.48 Forearm.configure :loose @22:33:44.66 Elbow.configure :loose @22:33:44.87 SC.configure :loose @22:33:45.05 SE.configure :default @22:33:45.13 CH.coast @22:33:45.23 CH.configure :default @22:33:45.29 PH.coast @22:33:45.41 PH.configure :default @22:33:45.49 All dwarf controllers running firmware version 3.85 @22:33:45.63 Valve.reset @22:33:46.02 Intake.select :close @22:33:46.12 Exhaust.select :close @22:33:46.20 CTV.dial :air @22:33:46.44 PSV.dial :air @22:33:46.56 CSV.dial :waste1 @22:33:46.83
Forearm.reconfigure :loose @22:33:46.87 SSV.dial :spit,avoiding: :exhaust @22:33:46.93 PS.reconfigure :default @22:33:47.20
Forearm.home.seek :retract @22:33:47.30 PS.home.seek 0ml @22:33:47.59 CS.reconfigure :fast @22:33:47.63 SS.reconfigure :default @22:33:47.84 CS.home.seek 0ml @22:33:47.92 SS.home.seek 0ml @22:33:48.13 Hand.close @22:33:48.19
Elbow.jog 1500 @22:33:48.79 Elbow.reconfigure :loose @22:33:48.93 Elbow.home.seek :clear @22:33:53.46 SE.reconfigure :default @22:33:53.55 SE.home.seek :down @22:33:54.29 SC.reconfigure :tight @22:33:54.38 SC.home.seek :initial @22:33:56.10 PS.stop @22:34:13.19
SC.configure :limp @22:34:13.29 Forearm.seek :retract @22:34:13.41 Elbow.seek :hammer @22:34:14.76 Forearm.reconfigure :tight @22:34:14.88 Forearm.seek retracted @22:34:15.50 PC.reconfigure :default @22:34:15.58
CC.reconfigure :default @22:34:15.76 PC.home.seek :open @22:34:15.80
CC.home.seek :open @22:34:19.51 Notice.email "At 22:34:19 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_11922.rb:14\n{1 => 20,\n 2 => 13,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:34:19, 22.9C, 26% humidity, 15.7psia, 14.055V, 0.196A, 0.196A avg, 2.75W",Subject: "Started DA_WCR_11922 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @22:34:19.55
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @22:34:19.61 Starting initialPurge @22:34:19.63 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:34:19.77 Hand.open @22:34:20.84 Elbow.reconfigure :tight @22:34:20.94 Elbow.seek FlushPuck::Garage @22:34:22.89 Elbow.reconfigure :loose @22:34:23.07 Forearm.reconfigure :loose @22:34:23.21 Forearm.seek :garage @22:34:24.38 Hand.close @22:34:25.26 Forearm.seek :retract @22:34:26.46 Elbow.reconfigure :tight @22:34:26.54 Elbow.seek CC @22:34:29.58 Elbow.reconfigure :loose @22:34:29.76 Forearm.reconfigure :tight @22:34:29.84 Forearm.seek CC @22:34:31.47 CC.seek :closed @22:34:35.82 Collection Clamp onSpring/offSpring for 8/25 samples, on/off torque 1.89 @22:34:36.00 Hand.open @22:34:36.90 Forearm.reconfigure :loose @22:34:36.98 Forearm.seek :retract @22:34:38.07 Flushing the Collection Valve::Manifold @22:34:38.29 CSV.dial CSR @22:34:39.88 Pulling 5ml of CSR.flush @22:34:39.90 CSR.select :flush @22:34:40.06 CS.reconfigure :default @22:34:40.12 CS.seek 5ml @22:34:53.71 delay 5 seconds @22:34:58.74 CSR.select :air @22:34:59.02 CS.reconfigure :fast @22:34:59.08 CS.seek 7.5ml @22:35:05.48 CSV.dial :waste @22:35:06.75 CS.reconfigure :default @22:35:06.83 CS.seek 0ml @22:35:26.62 Purging external Intake @22:35:26.91 CSV.dial :flush @22:35:27.40 CS.reconfigure :fast @22:35:27.48 CS.seek 10ml @22:35:32.76 upsync @22:35:49.96
delay 5 seconds @22:35:54.98 CSV.dial CBV @22:35:55.01
CBV.dial CSV @22:35:55.74 CTV.dial :intake @22:35:56.46 Intake.select :open @22:35:56.56 CS.seek 0ml @22:36:18.92 Intake.select :close @22:36:19.02 CTV.dial :air @22:36:19.76 Flushing upper manifold @22:36:19.87 CSV.dial :flush @22:36:20.59 CS.seek 5ml @22:36:32.21 delay 5 seconds @22:36:37.22 CSV.dial :air @22:36:37.72 CS.seek 7ml @22:36:42.90 CSV.dial CBV @22:36:42.92
CBV.dial CSV @22:36:43.91 CTV.dial :air @22:36:43.93 CS.seek 0ml @22:36:59.86 delay 7 seconds @22:37:06.89 CS.seek 3ml @22:37:14.24 CSV.dial :waste @22:37:14.68 CS.seek 0ml @22:37:22.09 CSV.dial :flush @22:37:22.63 CS.seek 3ml @22:37:29.99 delay 5 seconds @22:37:35.00 CSV.dial :air @22:37:35.51 CS.seek 5ml @22:37:40.71 CSV.dial :air @22:37:40.73 CS.seek 0ml @22:37:52.36 delay 7 seconds @22:37:59.39 CS.seek 3ml @22:38:06.77 CSV.dial :waste @22:38:07.48 CS.seek 0ml @22:38:14.80 Garaging FlushPuck in Collection Clamp @22:38:14.87 Elbow.reconfigure :tight @22:38:14.91 Elbow.seek CC @22:38:15.11 Elbow.reconfigure :loose @22:38:15.20 Forearm.seek CC @22:38:16.21 Hand.close @22:38:16.98 CC.seek :open @22:38:21.31 Forearm.seek :retract @22:38:22.29 Elbow.reconfigure :tight @22:38:22.34 Elbow.seek :garage @22:38:25.38 Elbow.reconfigure :loose @22:38:25.46 Forearm.reconfigure :tight @22:38:25.50 Forearm.seek :garage @22:38:26.64 Hand.open @22:38:27.41 Forearm.reconfigure :loose @22:38:27.86 Forearm.seek :retract @22:38:28.89 da sampling at most 1000ml, wcr at most 1000ml @22:38:28.92 Loading sh1 puck from tube 2 into Collection Clamp @22:38:28.94 DAfans.email "Started da in tube 2 at 22:38:28UTC09-Nov-22\n{1 => 20,\n 2 => 13,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:34:19, 22.9C, 26% humidity, 15.7psia, 14.055V, 0.196A, 0.196A avg, 2.75W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @22:38:29.18
SC.reconfigure :tight @22:38:29.22 SC.seek 2 @22:38:42.23 SC.configure :limp @22:38:42.67 Elbow.reconfigure :tight @22:38:42.76 Elbow.seek 2 @22:38:44.90 Forearm.reconfigure :tight @22:38:44.98 Forearm.seek 2 + 700 counts @22:38:46.15 SE.reconfigure :pick @22:38:46.25 SE.seek :beamBreak @22:38:48.75 SE.configure :default @22:38:49.05 Elbow.seek 2 @22:38:49.46 Forearm.seek 2 + 300 counts @22:38:49.71 Forearm.reconfigure :loose @22:38:49.79 Hand.close @22:38:50.52 SE.seek :down @22:38:50.94
Forearm.seek :retract @22:38:51.97 Elbow.seek CC @22:38:52.70 SE.stop @22:38:53.65
Elbow.reconfigure :loose @22:38:53.93 Forearm.reconfigure :tight @22:38:54.01 Forearm.seek CC @22:38:55.64 CC.seek :closed @22:38:59.98 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.70 @22:39:00.00 Hand.open @22:39:00.87 Forearm.reconfigure :loose @22:39:00.95 Forearm.seek :retract @22:39:02.00 collectSample 1000ml w/bubble point of 24psi @22:39:02.02 Priming 7ml @22:39:02.04 Intake.select :close @22:39:02.06 Exhaust.select :close @22:39:02.08 SS.hold @22:39:02.52 Calibrating pressure and temperature sensors @22:39:02.57 CTV.dial :air @22:39:02.60 SSV.dial :spit,avoiding: :exhaust @22:39:02.64 SS.reconfigure :fast @22:39:02.70 SS.seek 5ml @22:39:05.47 SSV.dial CBV @22:39:05.62
CBV.dial SSV @22:39:06.92 delay 3.8 seconds @22:39:10.93 SSV.dial :spit @22:39:12.27 SS.seek 0ml @22:39:14.88 SSV.dial CBV @22:39:16.20 CTV.dial :intake @22:39:16.99 SSV.dial CBV @22:39:17.04
CBV.dial SSV @22:39:17.07 Allowing 40s for 7.0ml of remaining 7.0ml @22:39:17.09 Intake.select :open @22:39:19.33 Intake @15.4psia, vacuum from 9.9 to 12.9psi @22:39:19.43 SS.reconfigure :inhaling 2.14ml/s @22:39:19.50 SS.seek 7ml @22:39:26.17 Stroke completed -- waiting for pressure to equalize ... @22:39:26.19 SS.reconfigure :exhale @22:39:26.34 SS.hold @22:39:31.03 Intake.select :close @22:39:31.24 SSV.dial :exhaust @22:39:32.01 Exhaust.select :open @22:39:32.17 SS.seek 0ml @22:39:36.06 Exhaust.select :close @22:39:36.20 Sampling 1000.0ml @22:39:36.24 Intake.select :close @22:39:36.26 Exhaust.select :close @22:39:36.28 SS.reconfigure :default @22:39:36.36 SS.hold @22:39:36.79 CTV.dial :intake @22:39:36.83 SSV.dial CBV @22:39:36.87
CBV.dial SSV @22:39:37.67 Allowing 130s for 25.0ml of remaining 1000.0ml @22:39:37.69 Intake.select :open @22:39:39.96 Intake @15.4psia, vacuum from 9.9 to 12.9psi @22:39:39.98 SS.reconfigure :inhaling 2.14ml/s @22:39:40.09 SS.seek 25ml @22:39:45.52 Vacuum too high -- waiting up to 40 seconds @22:39:45.55 SS.reconfigure :exhale @22:39:45.67 SS.hold @22:39:46.49 Intake @11.9psia, vacuum from 6.4 to 9.4psi @22:39:46.53 SS.reconfigure :inhaling 1.93ml/s @22:39:47.60 SS.seek 25ml @22:39:50.56 Vacuum too high -- waiting up to 40 seconds @22:39:50.58 SS.reconfigure :exhale @22:39:50.66 SS.hold @22:39:52.40 Intake @15.3psia, vacuum from 9.8 to 12.8psi @22:39:52.42 SS.reconfigure :inhaling 1.74ml/s @22:39:52.52 SS.seek 25ml @22:39:55.56 Vacuum too high -- waiting up to 40 seconds @22:39:55.58 SS.reconfigure :exhale @22:39:55.66 SS.hold @22:39:56.37 Intake @13.1psia, vacuum from 7.6 to 10.6psi @22:39:56.39 SS.reconfigure :inhaling 1.57ml/s @22:39:56.56 SS.seek 25ml @22:39:59.65 Vacuum too high -- waiting up to 40 seconds @22:39:59.69 SS.reconfigure :exhale @22:39:59.80 SS.hold @22:40:01.11 Intake @14.5psia, vacuum from 9.0 to 12.0psi @22:40:01.14 SS.reconfigure :inhaling 1.41ml/s @22:40:01.22 SS.seek 25ml @22:40:04.53 Vacuum too high -- waiting up to 40 seconds @22:40:04.55 SS.reconfigure :exhale @22:40:04.63 SS.hold @22:40:05.55 Intake @13.6psia, vacuum from 8.1 to 11.1psi @22:40:05.59 SS.reconfigure :inhaling 1.27ml/s @22:40:05.67 SS.seek 25ml @22:40:09.67 Vacuum too high -- waiting up to 40 seconds @22:40:09.69 SS.reconfigure :exhale @22:40:09.75 SS.hold @22:40:11.03 Intake @14.2psia, vacuum from 8.7 to 11.7psi @22:40:11.07 SS.reconfigure :inhaling 1.14ml/s @22:40:11.15 SS.seek 25ml @22:40:16.51 Stroke completed -- waiting for pressure to equalize ... @22:40:16.53 SS.reconfigure :exhale @22:40:16.61 SS.hold @22:40:18.53 Intake.select :close @22:40:18.78 SSV.dial :exhaust @22:40:19.58 Exhaust.select :open @22:40:19.72 SS.seek 0ml @22:40:28.56 Exhaust.select :close @22:40:28.70 SSV.dial CBV @22:40:28.76
CBV.dial SSV @22:40:29.53 Allowing 130s for 25.0ml of remaining 975.0ml @22:40:29.55 Intake.select :open @22:40:31.83 Intake @15.1psia, vacuum from 9.6 to 12.6psi @22:40:31.85 SS.reconfigure :inhaling 1.14ml/s @22:40:31.95 SS.seek 25ml @22:40:33.87 upsync @22:40:38.00
Vacuum too high -- waiting up to 40 seconds @22:40:38.01 SS.reconfigure :exhale @22:40:38.05 SS.hold @22:40:38.48 Intake @12.4psia, vacuum from 6.9 to 9.9psi @22:40:38.50 SS.reconfigure :inhaling 1.03ml/s @22:40:38.54 SS.seek 25ml @22:40:44.53 Vacuum too high -- waiting up to 40 seconds @22:40:44.54 SS.reconfigure :exhale @22:40:44.58 SS.hold @22:40:46.26 Intake @14.5psia, vacuum from 9.0 to 12.0psi @22:40:46.28 SS.reconfigure :inhaling 0.93ml/s @22:40:46.33 SS.seek 25ml @22:40:57.09 Vacuum too high -- waiting up to 40 seconds @22:40:57.10 SS.reconfigure :exhale @22:40:57.14 SS.hold @22:40:58.41 Intake @14.0psia, vacuum from 8.5 to 11.5psi @22:40:58.43 SS.reconfigure :inhaling 0.84ml/s @22:40:58.47 SS.seek 25ml @22:41:06.43 Stroke completed -- waiting for pressure to equalize ... @22:41:06.44 SS.reconfigure :exhale @22:41:06.48 SS.hold @22:41:08.44 Intake.select :close @22:41:08.57 SSV.dial :exhaust @22:41:09.31 Exhaust.select :open @22:41:09.41 SS.seek 0ml @22:41:18.69 Exhaust.select :close @22:41:18.79 SSV.dial CBV @22:41:18.82
CBV.dial SSV @22:41:19.57 Allowing 130s for 25.0ml of remaining 950.0ml @22:41:19.58 Intake.select :open @22:41:21.74 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:41:21.75 SS.reconfigure :inhaling 0.84ml/s @22:41:21.79 SS.seek 25ml @22:41:52.93 Vacuum too high -- waiting up to 40 seconds @22:41:52.94 SS.reconfigure :exhale @22:41:52.98 SS.hold @22:41:53.37 Intake @12.7psia, vacuum from 7.2 to 10.2psi @22:41:53.38 SS.reconfigure :inhaling 0.76ml/s @22:41:53.42 SS.seek 25ml @22:41:54.17 Stroke completed -- waiting for pressure to equalize ... @22:41:54.18 SS.reconfigure :exhale @22:41:54.22 SS.hold @22:41:55.77 Intake.select :close @22:41:55.91 SSV.dial :exhaust @22:41:56.63 Exhaust.select :open @22:41:56.74 SS.seek 0ml @22:42:05.53 Exhaust.select :close @22:42:05.64 SSV.dial CBV @22:42:05.66
CBV.dial SSV @22:42:06.40 Allowing 130s for 25.0ml of remaining 925.0ml @22:42:06.41 Intake.select :open @22:42:08.56 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:42:08.57 SS.reconfigure :inhaling 0.76ml/s @22:42:08.61 SS.seek 25ml @22:42:44.28 Stroke completed -- waiting for pressure to equalize ... @22:42:44.29 SS.reconfigure :exhale @22:42:44.33 SS.hold @22:42:46.37 Intake.select :close @22:42:46.51 SSV.dial :exhaust @22:42:47.71 Exhaust.select :open @22:42:47.81 SS.seek 0ml @22:42:56.72 Exhaust.select :close @22:42:56.83 SSV.dial CBV @22:42:56.85
CBV.dial SSV @22:42:57.60 Allowing 130s for 25.0ml of remaining 900.0ml @22:42:57.61 Intake.select :open @22:42:59.76 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:42:59.78 SS.reconfigure :inhaling 0.76ml/s @22:42:59.82 SS.seek 25ml @22:43:35.48 Stroke completed -- waiting for pressure to equalize ... @22:43:35.49 SS.reconfigure :exhale @22:43:35.54 SS.hold @22:43:38.05 Intake.select :close @22:43:38.19 SSV.dial :exhaust @22:43:38.91 Exhaust.select :open @22:43:39.01 SS.seek 0ml @22:43:47.84 Exhaust.select :close @22:43:47.95 SSV.dial CBV @22:43:47.97
CBV.dial SSV @22:43:48.71 Allowing 130s for 25.0ml of remaining 875.0ml @22:43:48.72 Intake.select :open @22:43:50.87 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:43:50.88 SS.reconfigure :inhaling 0.76ml/s @22:43:50.93 SS.seek 25ml @22:44:26.53 Stroke completed -- waiting for pressure to equalize ... @22:44:26.54 SS.reconfigure :exhale @22:44:26.58 SS.hold @22:44:28.53 Intake.select :close @22:44:28.66 SSV.dial :exhaust @22:44:29.39 Exhaust.select :open @22:44:29.50 SS.seek 0ml @22:44:38.48 Exhaust.select :close @22:44:38.59 SSV.dial CBV @22:44:38.61
CBV.dial SSV @22:44:39.36 Allowing 130s for 25.0ml of remaining 850.0ml @22:44:39.37 Intake.select :open @22:44:41.52 Intake @14.6psia, vacuum from 9.1 to 12.1psi @22:44:41.54 SS.reconfigure :inhaling 0.76ml/s @22:44:41.58 SS.seek 25ml @22:45:17.23 Stroke completed -- waiting for pressure to equalize ... @22:45:17.24 SS.reconfigure :exhale @22:45:17.28 SS.hold @22:45:20.16 Intake.select :close @22:45:20.30 SSV.dial :exhaust @22:45:21.03 Exhaust.select :open @22:45:21.13 SS.seek 0ml @22:45:30.11 Exhaust.select :close @22:45:30.21 SSV.dial CBV @22:45:30.24
CBV.dial SSV @22:45:30.97 Allowing 130s for 25.0ml of remaining 825.0ml @22:45:30.98 Intake.select :open @22:45:33.13 Intake @14.7psia, vacuum from 9.2 to 12.2psi @22:45:33.14 SS.reconfigure :inhaling 0.76ml/s @22:45:33.19 SS.seek 25ml @22:46:08.73 Stroke completed -- waiting for pressure to equalize ... @22:46:08.74 SS.reconfigure :exhale @22:46:08.78 SS.hold @22:46:11.66 Intake.select :close @22:46:11.80 SSV.dial :exhaust @22:46:12.52 Exhaust.select :open @22:46:12.63 SS.seek 0ml @22:46:21.51 Exhaust.select :close @22:46:21.61 SSV.dial CBV @22:46:21.64
CBV.dial SSV @22:46:22.37 Allowing 130s for 25.0ml of remaining 800.0ml @22:46:22.38 Intake.select :open @22:46:24.54 Intake @14.7psia, vacuum from 9.2 to 12.2psi @22:46:24.55 SS.reconfigure :inhaling 0.76ml/s @22:46:24.59 SS.seek 25ml @22:47:00.18 Stroke completed -- waiting for pressure to equalize ... @22:47:00.19 SS.reconfigure :exhale @22:47:00.23 SS.hold @22:47:03.67 Intake.select :close @22:47:03.81 SSV.dial :exhaust @22:47:04.53 Exhaust.select :open @22:47:04.63 SS.seek 0ml @22:47:13.59 Exhaust.select :close @22:47:13.69 SSV.dial CBV @22:47:13.73
CBV.dial SSV @22:47:14.43 Allowing 130s for 25.0ml of remaining 775.0ml @22:47:14.44 Intake.select :open @22:47:16.59 Intake @14.6psia, vacuum from 9.1 to 12.1psi @22:47:16.61 SS.reconfigure :inhaling 0.76ml/s @22:47:16.65 SS.seek 25ml @22:47:52.13 Stroke completed -- waiting for pressure to equalize ... @22:47:52.14 SS.reconfigure :exhale @22:47:52.18 SS.hold @22:47:56.24 Intake.select :close @22:47:56.38 SSV.dial :exhaust @22:47:57.09 Exhaust.select :open @22:47:57.19 SS.seek 0ml @22:48:06.18 Exhaust.select :close @22:48:06.28 SSV.dial CBV @22:48:06.33
CBV.dial SSV @22:48:07.03 Allowing 130s for 25.0ml of remaining 750.0ml @22:48:07.04 Intake.select :open @22:48:09.19 Intake @14.6psia, vacuum from 9.1 to 12.1psi @22:48:09.20 SS.reconfigure :inhaling 0.76ml/s @22:48:09.25 SS.seek 25ml @22:48:45.32 Stroke completed -- waiting for pressure to equalize ... @22:48:45.33 SS.reconfigure :exhale @22:48:45.37 SS.hold @22:48:48.93 Intake.select :close @22:48:49.07 SSV.dial :exhaust @22:48:49.79 Exhaust.select :open @22:48:49.89 SS.seek 0ml @22:48:59.22 Exhaust.select :close @22:48:59.33 SSV.dial CBV @22:48:59.35
CBV.dial SSV @22:49:00.09 Allowing 130s for 25.0ml of remaining 725.0ml @22:49:00.10 Intake.select :open @22:49:02.25 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:49:02.26 SS.reconfigure :inhaling 0.76ml/s @22:49:02.30 SS.seek 25ml @22:49:25.76 Vacuum too high -- waiting up to 40 seconds @22:49:25.77 SS.reconfigure :exhale @22:49:25.81 SS.hold @22:49:26.19 Intake @12.1psia, vacuum from 6.6 to 9.6psi @22:49:26.21 SS.reconfigure :inhaling 0.69ml/s @22:49:26.25 SS.seek 25ml @22:49:38.96 Stroke completed -- waiting for pressure to equalize ... @22:49:38.97 SS.reconfigure :exhale @22:49:39.01 SS.hold @22:49:42.45 Intake.select :close @22:49:42.58 SSV.dial :exhaust @22:49:43.29 Exhaust.select :open @22:49:43.39 SS.seek 0ml @22:49:52.69 Exhaust.select :close @22:49:52.79 SSV.dial CBV @22:49:52.82
CBV.dial SSV @22:49:53.55 Allowing 130s for 25.0ml of remaining 700.0ml @22:49:53.56 Intake.select :open @22:49:55.71 Intake @14.7psia, vacuum from 9.2 to 12.2psi @22:49:55.73 SS.reconfigure :inhaling 0.69ml/s @22:49:55.77 SS.seek 25ml @22:50:33.94 Stroke completed -- waiting for pressure to equalize ... @22:50:33.95 SS.reconfigure :exhale @22:50:33.99 SS.hold @22:50:37.55 Intake.select :close @22:50:37.68 SSV.dial :exhaust @22:50:38.41 Exhaust.select :open @22:50:38.51 SS.seek 0ml @22:50:47.81 Exhaust.select :close @22:50:47.92 SSV.dial CBV @22:50:47.94
CBV.dial SSV @22:50:48.68 Allowing 130s for 25.0ml of remaining 675.0ml @22:50:48.69 Intake.select :open @22:50:50.84 Intake @14.7psia, vacuum from 9.2 to 12.2psi @22:50:50.86 SS.reconfigure :inhaling 0.69ml/s @22:50:50.90 SS.seek 25ml @22:51:29.03 Stroke completed -- waiting for pressure to equalize ... @22:51:29.04 SS.reconfigure :exhale @22:51:29.08 SS.hold @22:51:33.14 Intake.select :close @22:51:33.28 SSV.dial :exhaust @22:51:34.00 Exhaust.select :open @22:51:34.11 SS.seek 0ml @22:51:43.11 Exhaust.select :close @22:51:43.21 SSV.dial CBV @22:51:43.25
CBV.dial SSV @22:51:43.97 Allowing 130s for 25.0ml of remaining 650.0ml @22:51:43.98 Intake.select :open @22:51:46.13 Intake @14.6psia, vacuum from 9.1 to 12.1psi @22:51:46.15 SS.reconfigure :inhaling 0.69ml/s @22:51:46.19 SS.seek 25ml @22:52:24.61 Stroke completed -- waiting for pressure to equalize ... @22:52:24.62 SS.reconfigure :exhale @22:52:24.66 SS.hold @22:52:30.18 Intake.select :close @22:52:30.32 SSV.dial :exhaust @22:52:31.05 Exhaust.select :open @22:52:31.15 SS.seek 0ml @22:52:40.54 Exhaust.select :close @22:52:40.65 SSV.dial CBV @22:52:40.67
CBV.dial SSV @22:52:41.41 Allowing 130s for 25.0ml of remaining 625.0ml @22:52:41.42 Intake.select :open @22:52:43.57 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:52:43.58 SS.reconfigure :inhaling 0.69ml/s @22:52:43.63 SS.seek 25ml @22:53:07.19 Vacuum too high -- waiting up to 40 seconds @22:53:07.20 SS.reconfigure :exhale @22:53:07.24 SS.hold @22:53:07.89 Intake @12.3psia, vacuum from 6.8 to 9.8psi @22:53:07.91 SS.reconfigure :inhaling 0.61ml/s @22:53:07.95 SS.seek 25ml @22:53:25.20 Stroke completed -- waiting for pressure to equalize ... @22:53:25.21 SS.reconfigure :exhale @22:53:25.25 SS.hold @22:53:29.31 Intake.select :close @22:53:29.45 SSV.dial :exhaust @22:53:30.17 Exhaust.select :open @22:53:30.28 SS.seek 0ml @22:53:39.68 Exhaust.select :close @22:53:39.79 SSV.dial CBV @22:53:39.84
CBV.dial SSV @22:53:40.53 Allowing 130s for 25.0ml of remaining 600.0ml @22:53:40.54 Intake.select :open @22:53:42.71 Intake @14.9psia, vacuum from 9.4 to 12.4psi @22:53:42.73 SS.reconfigure :inhaling 0.61ml/s @22:53:42.77 SS.seek 25ml @22:54:25.37 Stroke completed -- waiting for pressure to equalize ... @22:54:25.38 SS.reconfigure :exhale @22:54:25.42 SS.hold @22:54:30.94 Intake.select :close @22:54:31.07 SSV.dial :exhaust @22:54:31.78 Exhaust.select :open @22:54:31.88 SS.seek 0ml @22:54:40.87 Exhaust.select :close @22:54:40.97 SSV.dial CBV @22:54:41.01
CBV.dial SSV @22:54:41.71 Allowing 130s for 25.0ml of remaining 575.0ml @22:54:41.73 Intake.select :open @22:54:43.88 Intake @15.1psia, vacuum from 9.6 to 12.6psi @22:54:43.89 SS.reconfigure :inhaling 0.61ml/s @22:54:43.94 SS.seek 25ml @22:55:27.38 Stroke completed -- waiting for pressure to equalize ... @22:55:27.39 SS.reconfigure :exhale @22:55:27.43 SS.hold @22:55:32.95 Intake.select :close @22:55:33.09 SSV.dial :exhaust @22:55:33.81 Exhaust.select :open @22:55:33.91 SS.seek 0ml @22:55:42.88 Exhaust.select :close @22:55:42.99 SSV.dial CBV @22:55:43.01
CBV.dial SSV @22:55:43.74 Allowing 130s for 25.0ml of remaining 550.0ml @22:55:43.75 Intake.select :open @22:55:45.91 Intake @15.1psia, vacuum from 9.6 to 12.6psi @22:55:45.92 SS.reconfigure :inhaling 0.61ml/s @22:55:45.96 SS.seek 25ml @22:56:15.81 Vacuum too high -- waiting up to 40 seconds @22:56:15.82 SS.reconfigure :exhale @22:56:15.86 SS.hold @22:56:18.34 Intake @14.7psia, vacuum from 9.2 to 12.2psi @22:56:18.36 SS.reconfigure :inhaling 0.56ml/s @22:56:18.40 SS.seek 25ml @22:56:33.96 Stroke completed -- waiting for pressure to equalize ... @22:56:33.97 SS.reconfigure :exhale @22:56:34.01 SS.hold @22:56:38.75 Intake.select :close @22:56:38.88 SSV.dial :exhaust @22:56:39.60 Exhaust.select :open @22:56:39.71 SS.seek 0ml @22:56:48.72 Exhaust.select :close @22:56:48.83 SSV.dial CBV @22:56:48.85
CBV.dial SSV @22:56:49.59 Allowing 130s for 25.0ml of remaining 525.0ml @22:56:49.60 Intake.select :open @22:56:51.75 Intake @15.2psia, vacuum from 9.7 to 12.7psi @22:56:51.77 SS.reconfigure :inhaling 0.56ml/s @22:56:51.81 SS.seek 25ml @22:57:39.21 Stroke completed -- waiting for pressure to equalize ... @22:57:39.22 SS.reconfigure :exhale @22:57:39.71 SS.hold @22:57:45.15 Intake.select :close @22:57:45.29 SSV.dial :exhaust @22:57:46.02 Exhaust.select :open @22:57:46.13 SS.seek 0ml @22:57:55.10 Exhaust.select :close @22:57:55.20 SSV.dial CBV @22:57:55.25
CBV.dial SSV @22:57:55.95 Allowing 130s for 25.0ml of remaining 500.0ml @22:57:55.96 Intake.select :open @22:57:58.11 Intake @15.2psia, vacuum from 9.7 to 12.7psi @22:57:58.12 SS.reconfigure :inhaling 0.56ml/s @22:57:58.17 SS.seek 25ml @22:58:46.62 Stroke completed -- waiting for pressure to equalize ... @22:58:46.63 SS.reconfigure :exhale @22:58:46.68 SS.hold @22:58:54.03 Intake.select :close @22:58:54.17 SSV.dial :exhaust @22:58:54.89 Exhaust.select :open @22:58:55.00 SS.seek 0ml @22:59:04.02 Exhaust.select :close @22:59:04.12 SSV.dial CBV @22:59:04.17
CBV.dial SSV @22:59:04.88 Allowing 130s for 25.0ml of remaining 475.0ml @22:59:04.89 Intake.select :open @22:59:07.05 Intake @15.3psia, vacuum from 9.8 to 12.8psi @22:59:07.07 SS.reconfigure :inhaling 0.56ml/s @22:59:07.12 SS.seek 25ml @22:59:51.55 Vacuum too high -- waiting up to 40 seconds @22:59:51.56 SS.reconfigure :exhale @22:59:51.60 SS.hold @22:59:54.48 Intake @14.1psia, vacuum from 8.6 to 11.6psi @22:59:54.50 SS.reconfigure :inhaling 0.50ml/s @22:59:54.54 SS.seek 25ml @22:59:59.65 Stroke completed -- waiting for pressure to equalize ... @22:59:59.66 SS.reconfigure :exhale @22:59:59.70 SS.hold @23:00:03.77 Intake.select :close @23:00:03.91 SSV.dial :exhaust @23:00:04.64 Exhaust.select :open @23:00:04.75 SS.seek 0ml @23:00:14.12 Exhaust.select :close @23:00:14.23 SSV.dial CBV @23:00:14.25
CBV.dial SSV @23:00:14.99 Allowing 130s for 25.0ml of remaining 450.0ml @23:00:15.00 Intake.select :open @23:00:17.15 Intake @15.2psia, vacuum from 9.7 to 12.7psi @23:00:17.16 SS.reconfigure :inhaling 0.50ml/s @23:00:17.21 SS.seek 25ml @23:01:11.57 Stroke completed -- waiting for pressure to equalize ... @23:01:11.58 SS.reconfigure :exhale @23:01:11.62 SS.hold @23:01:17.14 Intake.select :close @23:01:17.27 SSV.dial :exhaust @23:01:18.00 Exhaust.select :open @23:01:18.10 SS.seek 0ml @23:01:27.06 Exhaust.select :close @23:01:27.16 SSV.dial CBV @23:01:27.20
CBV.dial SSV @23:01:27.94 Allowing 130s for 25.0ml of remaining 425.0ml @23:01:27.95 Intake.select :open @23:01:30.10 Intake @15.2psia, vacuum from 9.7 to 12.7psi @23:01:30.11 SS.reconfigure :inhaling 0.50ml/s @23:01:30.61 SS.seek 25ml @23:02:25.48 Stroke completed -- waiting for pressure to equalize ... @23:02:25.49 SS.reconfigure :exhale @23:02:25.53 SS.hold @23:02:32.94 Intake.select :close @23:02:33.08 SSV.dial :exhaust @23:02:33.81 Exhaust.select :open @23:02:33.91 SS.seek 0ml @23:02:42.90 Exhaust.select :close @23:02:43.00 SSV.dial CBV @23:02:43.03
CBV.dial SSV @23:02:43.76 Allowing 130s for 25.0ml of remaining 400.0ml @23:02:43.77 Intake.select :open @23:02:45.92 Intake @15.4psia, vacuum from 9.9 to 12.9psi @23:02:45.94 SS.reconfigure :inhaling 0.50ml/s @23:02:45.98 SS.seek 25ml @23:03:41.41 Stroke completed -- waiting for pressure to equalize ... @23:03:41.42 SS.reconfigure :exhale @23:03:41.46 SS.hold @23:03:48.85 Intake.select :close @23:03:48.98 SSV.dial :exhaust @23:03:49.71 Exhaust.select :open @23:03:49.82 SS.seek 0ml @23:03:59.13 Exhaust.select :close @23:03:59.23 SSV.dial CBV @23:03:59.27
CBV.dial SSV @23:03:59.99 Allowing 130s for 25.0ml of remaining 375.0ml @23:04:00.00 Intake.select :open @23:04:02.15 Intake @15.1psia, vacuum from 9.6 to 12.6psi @23:04:02.17 SS.reconfigure :inhaling 0.50ml/s @23:04:02.21 SS.seek 25ml @23:04:57.96 Stroke completed -- waiting for pressure to equalize ... @23:04:57.97 SS.reconfigure :exhale @23:04:58.01 SS.hold @23:05:06.47 Intake.select :close @23:05:06.61 SSV.dial :exhaust @23:05:07.33 Exhaust.select :open @23:05:07.44 SS.seek 0ml @23:05:16.71 Exhaust.select :close @23:05:16.81 SSV.dial CBV @23:05:16.85
CBV.dial SSV @23:05:17.55 Allowing 130s for 25.0ml of remaining 350.0ml @23:05:17.56 Intake.select :open @23:05:19.71 Intake @15.1psia, vacuum from 9.6 to 12.6psi @23:05:19.73 SS.reconfigure :inhaling 0.50ml/s @23:05:19.77 SS.seek 25ml @23:06:07.85 Vacuum too high -- waiting up to 40 seconds @23:06:07.86 SS.reconfigure :exhale @23:06:07.90 SS.hold @23:06:11.95 Intake @14.9psia, vacuum from 9.4 to 12.4psi @23:06:11.96 SS.reconfigure :inhaling 0.46ml/s @23:06:12.00 SS.seek 25ml @23:06:21.16 Stroke completed -- waiting for pressure to equalize ... @23:06:21.17 SS.reconfigure :exhale @23:06:21.21 SS.hold @23:06:27.29 Intake.select :close @23:06:27.43 SSV.dial :exhaust @23:06:28.16 Exhaust.select :open @23:06:28.27 SS.seek 0ml @23:06:37.25 Exhaust.select :close @23:06:37.36 SSV.dial CBV @23:06:37.38
CBV.dial SSV @23:06:38.13 Allowing 130s for 25.0ml of remaining 325.0ml @23:06:38.14 Intake.select :open @23:06:40.29 Intake @15.0psia, vacuum from 9.5 to 12.5psi @23:06:40.31 SS.reconfigure :inhaling 0.46ml/s @23:06:40.35 SS.seek 25ml @23:07:43.22 Stroke completed -- waiting for pressure to equalize ... @23:07:43.23 SS.reconfigure :exhale @23:07:43.27 SS.hold @23:07:51.83 Intake.select :close @23:07:51.97 SSV.dial :exhaust @23:07:52.69 Exhaust.select :open @23:07:52.79 SS.seek 0ml @23:08:01.78 Exhaust.select :close @23:08:01.88 SSV.dial CBV @23:08:01.91
CBV.dial SSV @23:08:02.64 Allowing 130s for 25.0ml of remaining 300.0ml @23:08:02.65 Intake.select :open @23:08:04.80 Intake @15.1psia, vacuum from 9.6 to 12.6psi @23:08:04.82 SS.reconfigure :inhaling 0.46ml/s @23:08:04.86 SS.seek 25ml @23:09:07.60 Stroke completed -- waiting for pressure to equalize ... @23:09:07.61 SS.reconfigure :exhale @23:09:07.66 SS.hold @23:09:17.30 Intake.select :close @23:09:17.43 SSV.dial :exhaust @23:09:18.14 Exhaust.select :open @23:09:18.25 SS.seek 0ml @23:09:27.64 Exhaust.select :close @23:09:27.74 SSV.dial CBV @23:09:27.78
CBV.dial SSV @23:09:28.48 Allowing 130s for 25.0ml of remaining 275.0ml @23:09:28.49 Intake.select :open @23:09:30.64 Intake @15.1psia, vacuum from 9.6 to 12.6psi @23:09:30.66 SS.reconfigure :inhaling 0.46ml/s @23:09:30.70 SS.seek 25ml @23:10:25.63 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @23:10:25.67 SS.hold @23:10:30.41 Intake @14.7psia, vacuum from 9.2 to 12.2psi @23:10:30.43 SS.reconfigure :inhaling 0.41ml/s @23:10:30.47 SS.seek 25ml @23:10:40.43 Stroke completed -- waiting for pressure to equalize ... @23:10:40.44 SS.reconfigure :exhale @23:10:40.48 SS.hold @23:10:46.86 Intake.select :close @23:10:46.99 SSV.dial :exhaust @23:10:47.71 Exhaust.select :open @23:10:47.82 SS.seek 0ml @23:10:56.82 Exhaust.select :close @23:10:56.92 SSV.dial CBV @23:10:56.95
CBV.dial SSV @23:10:57.68 Allowing 130s for 25.0ml of remaining 250.0ml @23:10:57.69 Intake.select :open @23:10:59.84 Intake @15.2psia, vacuum from 9.7 to 12.7psi @23:10:59.86 SS.reconfigure :inhaling 0.41ml/s @23:10:59.90 SS.seek 25ml @23:12:10.57 Stroke completed -- waiting for pressure to equalize ... @23:12:11.02 SS.reconfigure :exhale @23:12:11.07 SS.hold @23:12:20.81 Intake.select :close @23:12:20.95 SSV.dial :exhaust @23:12:21.68 Exhaust.select :open @23:12:21.78 SS.seek 0ml @23:12:31.08 Exhaust.select :close @23:12:31.19 SSV.dial CBV @23:12:31.21
CBV.dial SSV @23:12:31.96 Allowing 130s for 25.0ml of remaining 225.0ml @23:12:31.97 Intake.select :open @23:12:34.12 Intake @15.3psia, vacuum from 9.8 to 12.8psi @23:12:34.14 SS.reconfigure :inhaling 0.41ml/s @23:12:34.18 SS.seek 25ml @23:13:44.52 Stroke completed -- waiting for pressure to equalize ... @23:13:44.53 SS.reconfigure :exhale @23:13:44.57 SS.hold @23:13:55.57 Intake.select :close @23:13:55.71 SSV.dial :exhaust @23:13:56.43 Exhaust.select :open @23:13:56.54 SS.seek 0ml @23:14:05.83 Exhaust.select :close @23:14:05.93 SSV.dial CBV @23:14:05.96
CBV.dial SSV @23:14:06.69 Allowing 130s for 25.0ml of remaining 200.0ml @23:14:06.70 Intake.select :open @23:14:08.86 Intake @15.1psia, vacuum from 9.6 to 12.6psi @23:14:08.88 SS.reconfigure :inhaling 0.41ml/s @23:14:08.92 SS.seek 25ml @23:14:59.49 Vacuum too high -- waiting up to 40 seconds @23:14:59.50 SS.reconfigure :exhale @23:14:59.54 SS.hold @23:15:05.04 Intake @15.2psia, vacuum from 9.7 to 12.7psi @23:15:05.06 SS.reconfigure :inhaling 0.37ml/s @23:15:05.10 SS.seek 25ml @23:15:27.05 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @23:15:27.09 SS.hold @23:15:35.54 Intake.select :close @23:15:35.68 SSV.dial :exhaust @23:15:36.41 Exhaust.select :open @23:15:36.51 SS.seek 0ml @23:15:45.55 Exhaust.select :close @23:15:45.65 SSV.dial CBV @23:15:45.68
CBV.dial SSV @23:15:46.42 Allowing 130s for 25.0ml of remaining 175.0ml @23:15:46.43 Intake.select :open @23:15:48.59 Intake @15.2psia, vacuum from 9.7 to 12.7psi @23:15:48.60 SS.reconfigure :inhaling 0.37ml/s @23:15:48.64 SS.seek 25ml @23:17:07.78 Stroke completed -- waiting for pressure to equalize ... @23:17:07.79 SS.reconfigure :exhale @23:17:07.83 SS.hold @23:17:19.30 Intake.select :close @23:17:19.44 SSV.dial :exhaust @23:17:20.17 Exhaust.select :open @23:17:20.28 SS.seek 0ml @23:17:29.70 Exhaust.select :close @23:17:29.80 SSV.dial CBV @23:17:29.83
CBV.dial SSV @23:17:30.56 Allowing 130s for 25.0ml of remaining 150.0ml @23:17:30.57 Intake.select :open @23:17:32.72 Intake @15.2psia, vacuum from 9.7 to 12.7psi @23:17:32.74 SS.reconfigure :inhaling 0.37ml/s @23:17:32.78 SS.seek 25ml @23:18:50.51 Stroke completed -- waiting for pressure to equalize ... @23:18:50.52 SS.reconfigure :exhale @23:18:50.56 SS.hold @23:19:03.13 Intake.select :close @23:19:03.27 SSV.dial :exhaust @23:19:03.99 Exhaust.select :open @23:19:04.10 SS.seek 0ml @23:19:13.13 Exhaust.select :close @23:19:13.24 SSV.dial CBV @23:19:13.26
CBV.dial SSV @23:19:14.01 Allowing 130s for 25.0ml of remaining 125.0ml @23:19:14.02 Intake.select :open @23:19:16.17 Intake @15.4psia, vacuum from 9.9 to 12.9psi @23:19:16.19 SS.reconfigure :inhaling 0.37ml/s @23:19:16.23 SS.seek 25ml @23:20:24.23 Vacuum too high -- waiting up to 40 seconds @23:20:24.24 SS.reconfigure :exhale @23:20:24.28 SS.hold @23:20:31.72 Intake @14.9psia, vacuum from 9.4 to 12.4psi @23:20:31.73 SS.reconfigure :inhaling 0.33ml/s @23:20:31.78 SS.seek 25ml @23:20:44.24 Stroke completed -- waiting for pressure to equalize ... @23:20:44.25 SS.reconfigure :exhale @23:20:44.29 SS.hold @23:20:53.96 Intake.select :close @23:20:54.10 SSV.dial :exhaust @23:20:54.82 Exhaust.select :open @23:20:54.92 SS.seek 0ml @23:21:04.21 Exhaust.select :close @23:21:04.31 SSV.dial CBV @23:21:04.36
CBV.dial SSV @23:21:05.05 Allowing 130s for 25.0ml of remaining 100.0ml @23:21:05.06 Intake.select :open @23:21:07.21 Intake @15.3psia, vacuum from 9.8 to 12.8psi @23:21:07.23 SS.reconfigure :inhaling 0.33ml/s @23:21:07.27 SS.seek 25ml @23:22:33.41 Stroke completed -- waiting for pressure to equalize ... @23:22:33.42 SS.reconfigure :exhale @23:22:33.46 SS.hold @23:22:47.69 Intake.select :close @23:22:47.83 SSV.dial :exhaust @23:22:48.56 Exhaust.select :open @23:22:48.67 SS.seek 0ml @23:22:58.14 Exhaust.select :close @23:22:58.25 SSV.dial CBV @23:22:58.27
CBV.dial SSV @23:22:59.02 Allowing 130s for 25.0ml of remaining 75.0ml @23:22:59.03 Intake.select :open @23:23:01.18 Intake @15.2psia, vacuum from 9.7 to 12.7psi @23:23:01.20 SS.reconfigure :inhaling 0.33ml/s @23:23:01.24 SS.seek 25ml @23:24:28.28 Stroke completed -- waiting for pressure to equalize ... @23:24:28.29 SS.reconfigure :exhale @23:24:28.33 SS.hold @23:24:42.72 Intake.select :close @23:24:42.86 SSV.dial :exhaust @23:24:43.58 Exhaust.select :open @23:24:43.68 SS.seek 0ml @23:24:53.04 Exhaust.select :close @23:24:53.15 SSV.dial CBV @23:24:53.17
CBV.dial SSV @23:24:53.91 Allowing 130s for 25.0ml of remaining 50.0ml @23:24:53.92 Intake.select :open @23:24:56.07 Intake @15.2psia, vacuum from 9.7 to 12.7psi @23:24:56.08 SS.reconfigure :inhaling 0.33ml/s @23:24:56.12 SS.seek 25ml @23:26:17.25 Vacuum too high -- waiting up to 40 seconds @23:26:17.26 SS.reconfigure :exhale @23:26:17.30 SS.hold @23:26:23.77 Intake @14.4psia, vacuum from 8.9 to 11.9psi @23:26:23.79 SS.reconfigure :inhaling 0.30ml/s @23:26:23.83 SS.seek 25ml @23:26:32.76 Stroke completed -- waiting for pressure to equalize ... @23:26:32.77 SS.reconfigure :exhale @23:26:32.81 SS.hold @23:26:43.81 Intake.select :close @23:26:43.95 SSV.dial :exhaust @23:26:44.67 Exhaust.select :open @23:26:44.77 SS.seek 0ml @23:26:53.77 Exhaust.select :close @23:26:53.88 SSV.dial CBV @23:26:53.91
CBV.dial SSV @23:26:54.65 Allowing 130s for 25.0ml of remaining 25.0ml @23:26:54.66 Intake.select :open @23:26:56.81 Intake @15.3psia, vacuum from 9.8 to 12.8psi @23:26:56.83 SS.reconfigure :inhaling 0.30ml/s @23:26:56.87 SS.seek 24.96ml @23:28:33.75 Stroke completed -- waiting for pressure to equalize ... @23:28:33.76 SS.reconfigure :exhale @23:28:33.80 SS.hold @23:28:50.64 Intake.select :close @23:28:50.78 SSV.dial :exhaust @23:28:51.51 Exhaust.select :open @23:28:51.61 SS.seek 0ml @23:29:01.00 Exhaust.select :close @23:29:01.11 Sampled 1000.0ml @23:29:01.12 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @23:29:01.13 Intake.select :close @23:29:01.19 CSV.dial :waste @23:29:01.25 CS.seek 0ml @23:29:01.32 SSV.dial CBV,avoiding: :exhaust @23:29:01.35 CSV.dial :air @23:29:01.40
CBV.dial SSV @23:29:02.13 CS.seek 10ml @23:29:03.18
CTV.dial :air @23:29:03.90 delay 15 seconds @23:29:18.92 SSV.dial :spit,avoiding: :exhaust @23:29:20.20 CTV.dial CSV,avoiding: :intake @23:29:20.23
SS.seek 0ml @23:29:20.39 SSV.dial CBV,avoiding: :exhaust @23:29:24.81 CBV.dial between SSV and waste2 @23:29:25.18 CTV.dial CSV,avoiding: :air @23:29:25.21
CSV.dial CTV @23:29:25.64 CS.reconfigure :pressurize @23:29:25.69 CS.seek 0ml @23:29:44.41 BubblePoint: Intake @40.4psia, Outlet @16.1psia @23:29:44.42 CBV.dial SSV @23:29:44.73 SS.reconfigure :default @23:29:44.78 SS.seek 10ml @23:29:50.21 delay 15 seconds @23:30:05.32 Evacuated: Intake @16.9psia, Outlet @10.4psia @23:30:05.33 SSV.dial :spit,avoiding: :exhaust @23:30:06.62 SS.reconfigure :exhale @23:30:06.66 SS.seek 0ml @23:30:12.42 SS.reconfigure :fast @23:30:12.46 SS.seek 5ml @23:30:15.26 SSV.dial CBV,avoiding: :exhaust @23:30:15.28
CBV.dial SSV @23:30:16.57 CTV.dial :air,avoiding: :intake @23:30:17.81 CSV.dial CTV @23:30:17.84
SS.seek 10ml @23:30:18.84 CSV.dial :air @23:30:19.29 CS.reconfigure :fast @23:30:19.34 CS.seek 0ml @23:30:20.50
SSV.dial :spit,avoiding: :exhaust @23:30:21.81 SS.reconfigure :exhale @23:30:21.85 SS.seek 0ml @23:30:26.24 CS.stop @23:30:27.45
SS.reconfigure :fast @23:30:27.49 SS.seek 5ml @23:30:30.38 SSV.dial CBV,avoiding: :exhaust @23:30:30.41
CBV.dial SSV @23:30:32.70 SSV.dial :spit,avoiding: :exhaust @23:30:33.98 SS.reconfigure :default @23:30:34.02 SS.seek 0ml @23:30:37.36 SS.stop @23:30:37.40 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @23:30:37.47 CSV.dial :waste @23:30:37.58 SSV.dial CBV,avoiding: :exhaust @23:30:37.61
CBV.dial SSV @23:30:38.20 CS.seek 0ml @23:30:38.27 CSV.dial :air @23:30:38.90
CTV.dial :air @23:30:38.91 delay 15 seconds @23:30:38.98 CS.seek 10ml @23:30:53.93
SSV.dial :spit,avoiding: :exhaust @23:30:55.23 CTV.dial CSV,avoiding: :intake @23:30:55.26
SS.reconfigure :exhale @23:30:55.34 SS.seek 0ml @23:30:55.52 SSV.dial CBV,avoiding: :exhaust @23:31:01.70 CBV.dial between SSV and waste2 @23:31:02.09 CTV.dial CSV,avoiding: :air @23:31:02.11
CSV.dial CTV @23:31:02.54 CS.reconfigure :pressurize @23:31:02.59 CS.seek 0ml @23:31:23.74 BubblePoint: Intake @39.5psia, Outlet @15.3psia @23:31:23.75 CBV.dial SSV @23:31:24.07 SS.reconfigure :default @23:31:24.12 SS.seek 10ml @23:31:29.78 delay 15 seconds @23:31:45.33 Evacuated: Intake @18.9psia, Outlet @12.3psia @23:31:45.34 SSV.dial :spit,avoiding: :exhaust @23:31:46.63 SS.reconfigure :exhale @23:31:46.67 SS.seek 0ml @23:31:52.20 SS.reconfigure :fast @23:31:52.24 SS.seek 5ml @23:31:54.86 SSV.dial CBV,avoiding: :exhaust @23:31:54.89
CBV.dial SSV @23:31:56.18 CTV.dial :air,avoiding: :intake @23:31:57.45 CSV.dial CTV @23:31:57.48
SS.seek 10ml @23:31:58.48 CSV.dial :air @23:31:58.93 CS.reconfigure :fast @23:31:58.98 CS.seek 0ml @23:31:59.99
SSV.dial :spit,avoiding: :exhaust @23:32:01.29 SS.reconfigure :exhale @23:32:01.33 SS.seek 0ml @23:32:03.77 CS.stop @23:32:06.67
SS.reconfigure :fast @23:32:06.71 SS.seek 5ml @23:32:09.49 SSV.dial CBV,avoiding: :exhaust @23:32:09.52
CBV.dial SSV @23:32:11.81 SSV.dial :spit,avoiding: :exhaust @23:32:13.08 SS.reconfigure :default @23:32:13.13 SS.seek 0ml @23:32:16.48 SS.stop @23:32:16.52 Lysing: Adding 2.0ml AqMeOH @23:32:16.53 CBV.dial between CSV and waste1,avoiding: :DBV @23:32:17.96 CTV.dial CSV @23:32:19.21 CSV.dial CSR @23:32:19.98 Pulling 1ml of CSR.AqMeOH @23:32:19.99 CSR.select :AqMeOH @23:32:20.10 CS.reconfigure :default @23:32:20.14 CS.seek 1ml @23:32:23.55 delay 5 seconds @23:32:28.57 CSR.select :air @23:32:28.72 CS.seek 6ml @23:32:42.08 delay 2 seconds @23:32:44.10 CSV.dial :waste2 @23:32:44.64 CS.reconfigure :fast @23:32:44.69 CS.seek 0ml @23:32:58.43 CSV.dial CSR @23:32:58.89 Pulling 1.999ml of CSR.AqMeOH CSR.select :AqMeOH @23:32:59.00 CS.reconfigure :default @23:32:59.04 CS.seek 2ml @23:33:04.90 delay 5 seconds @23:33:09.91 CSR.select :air @23:33:10.07 CS.seek 5ml @23:33:18.49 delay 2 seconds @23:33:20.50 CSV.dial CTV @23:33:20.95 CS.seek 1ml @23:33:31.99 Before lysing Collection Puck Heater at 18.5C/39.0psia @23:33:32.01 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @23:33:32.03 CH.seek 70C,8:00 @23:35:16.70 CH.hold 10:00,70C @23:35:17.36 Begun lysing Collection Puck Heater at 68.1C/40.8psia @23:45:17.36 Ended lysing Collection Puck Heater at 70C/42.4psia @23:45:17.37 CS.seek 0ml @23:45:20.76 CTV.dial between spare and air,avoiding: :intake @23:45:21.74 CSV.dial :air @23:45:22.18 CS.seek 5ml @23:45:35.65 CSV.dial CTV @23:45:36.20 delay 2 seconds @23:45:38.22 CSV.dial :air @23:45:38.64 delay 2 seconds @23:45:40.66 CSV.dial CTV @23:45:41.09 delay 2 seconds @23:45:43.11 CSV.dial :air @23:45:43.56 delay 2 seconds @23:45:45.58 CS.seek 0ml @23:45:59.02 Puck @67.0psia after final lysis pressurization @23:45:59.03 Recovering Lysate into Collection syringe @23:45:59.04 CSV.dial CBV @23:46:00.01 CBV.dial CSV @23:46:00.37 CS.seek 5ml @23:46:13.81 delay 5 seconds @23:46:18.83 CTV.dial :air @23:46:19.34 CS.seek 7ml @23:46:25.19 delay 5 seconds @23:46:30.20 CSV.dial :air2 @23:46:30.64 CS.seek 10ml @23:46:39.09 CSV.dial between air and CTV,avoiding: CBV @23:46:40.21 Lysing Complete @23:46:40.27 Storing sh1 puck in Collection Clamp to tube 1 @23:46:40.43 SC.reconfigure :tight @23:46:40.47 SC.seek 1 @23:46:44.08 SC.configure :limp @23:46:44.27 Elbow.reconfigure :tight @23:46:44.31 Elbow.seek 1 @23:46:46.13 Forearm.reconfigure :tight @23:46:46.17 Forearm.seek 1 + 700 counts @23:46:47.13 SE.reconfigure :pick @23:46:47.18 SE.seek :beamBreak @23:46:49.02 SE.configure :default @23:46:49.17 Elbow.reconfigure :limp @23:46:49.21 Forearm.reconfigure :loose @23:46:49.25 Forearm.seek 1 @23:46:49.67 Elbow.hold @23:46:49.73 Elbow.reconfigure :tight @23:46:49.81 SE.seek 19.34 Pucks below Top Plate @23:46:50.31 Forearm.force -20 @23:46:50.34 Forearm.seek 1 + 312 counts @23:46:50.66 Forearm.reconfigure :tight @23:46:50.70 Forearm.seek 1 + 700 counts @23:46:51.00 SE.seek 20.34 Pucks below Top Plate @23:46:51.57 Forearm.reconfigure :loose @23:46:51.61 Forearm.seek :retract @23:46:52.63 Elbow.seek CC @23:46:54.26 Elbow.reconfigure :loose @23:46:54.34 Forearm.seek CC @23:46:55.34 Hand.close @23:46:56.09 CC.seek :open @23:47:00.52 Forearm.seek :retract @23:47:01.48 Elbow.reconfigure :tight @23:47:01.52 Elbow.seek 1 @23:47:03.34 Elbow.reconfigure :limp @23:47:03.38 Forearm.seek 1 @23:47:04.50 Elbow.hold @23:47:04.56 Elbow.reconfigure :tight @23:47:04.60 Hand.open @23:47:05.46 Forearm.force -20 @23:47:05.49 Forearm.seek 1 + 317 counts @23:47:05.85 Forearm.reconfigure :tight @23:47:05.89 Forearm.seek 1 + 700 counts @23:47:06.28 SE.seek :down @23:47:07.41
Forearm.reconfigure :loose @23:47:07.45 Forearm.seek :retract @23:47:07.61 SE.stop @23:47:08.42
Tranferring DA @23:47:08.43 Beginning samplePassC @23:47:08.90 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @23:47:09.09 Elbow.seek FlushPuck::Garage @23:47:11.00 Elbow.reconfigure :loose @23:47:11.09 Forearm.seek :garage @23:47:12.17 Hand.close @23:47:12.83 Forearm.seek :retract @23:47:13.89 Elbow.reconfigure :tight @23:47:13.93 Elbow.seek CC @23:47:16.76 Elbow.reconfigure :loose @23:47:16.85 Forearm.reconfigure :tight @23:47:16.89 Forearm.seek CC @23:47:18.19 CC.seek :closed @23:47:22.42 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.91 @23:47:22.49 Hand.open @23:47:23.27 Forearm.reconfigure :loose @23:47:23.31 Forearm.seek :retract @23:47:24.32 PSV.dial PSR @23:47:25.63 Pulling 1.5ml of PSR.flush @23:47:25.64 PSR.select :flush @23:47:25.76 PS.seek 1.5ml @23:47:41.74 delay 5 seconds @23:47:46.76 PSV.dial :waste1 @23:47:47.19 PS.seek 0ml @23:48:03.17 PSV.dial PSR @23:48:03.61 PSR.select :air @23:48:03.72 PS.seek 2.5ml @23:48:29.92 PRV.dial CBV,avoiding: :ab2 @23:48:29.95
CBV.dial PRV @23:48:31.58 PSV.dial :mixloop @23:48:32.56 CTV.dial :air,avoiding: :intake @23:48:32.57 PTV.dial :PRVmixing @23:48:33.80 PS.seek 0ml @23:49:00.04 PSV.dial :air @23:49:00.47 PS.seek 2.5ml @23:49:26.70 PSV.dial :mixloop @23:49:27.14 PS.seek 0ml @23:49:53.38 PSV.dial :air @23:49:53.83 PS.seek 2.5ml @23:50:19.98 PSV.dial :mixloop @23:50:20.42 PS.seek 0ml @23:50:46.67 Garaging FlushPuck in Collection Clamp @23:50:46.74 Elbow.reconfigure :tight @23:50:46.78 Elbow.seek CC @23:50:46.89 Elbow.reconfigure :loose @23:50:46.98 Forearm.seek CC @23:50:47.93 Hand.close @23:50:48.59 CC.seek :open @23:50:52.91 Forearm.seek :retract @23:50:53.91 Elbow.reconfigure :tight @23:50:53.95 Elbow.seek :garage @23:50:56.98 Elbow.reconfigure :loose @23:50:57.07 Forearm.reconfigure :tight @23:50:57.11 Forearm.seek :garage @23:50:58.21 Hand.open @23:50:59.02 Forearm.reconfigure :loose @23:50:59.06 Forearm.seek :retract @23:51:00.11 PSV.dial :mixloop @23:51:00.13 PTV.dial :PRVmixing @23:51:00.14 PRV.dial CBV @23:51:00.16
CBV.dial PRV @23:51:00.18 CTV.dial :air,avoiding: :intake @23:51:00.19 Loading lyfil puck from tube 2 into Collection Clamp @23:51:00.41 SC.reconfigure :tight @23:51:00.45 SC.seek 2 @23:51:04.01 SC.configure :limp @23:51:04.20 Elbow.reconfigure :tight @23:51:04.24 Elbow.seek 2 @23:51:06.79 Forearm.reconfigure :tight @23:51:06.83 Forearm.seek 2 + 700 counts @23:51:07.99 SE.reconfigure :pick @23:51:08.04 SE.seek :beamBreak @23:51:10.40 SE.configure :default @23:51:10.55 Elbow.seek 2 @23:51:10.77 Forearm.seek 2 + 300 counts @23:51:11.08 Forearm.reconfigure :loose @23:51:11.12 Hand.close @23:51:11.72 SE.seek :down @23:51:11.91
Forearm.seek :retract @23:51:12.90 Elbow.seek CC @23:51:13.75 SE.stop @23:51:14.50
Elbow.reconfigure :loose @23:51:14.59 Forearm.reconfigure :tight @23:51:14.63 Forearm.seek CC @23:51:15.94 CC.seek :closed @23:51:20.17 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.64 @23:51:20.18 Hand.open @23:51:21.01 Forearm.reconfigure :loose @23:51:21.05 Forearm.seek :retract @23:51:22.07 CTV.dial CSV,avoiding: :intake @23:51:23.33 CSV.dial CTV @23:51:23.64 PS.seek 2.5ml @23:51:49.88 CS.seek 5.501ml @23:52:01.99 delay 8 seconds @23:52:10.01 PSV.dial :air @23:52:10.46 delay 2 seconds @23:52:12.48 PSV.dial :waste2 @23:52:13.20 PS.seek 0ml @23:52:39.44 CBV.dial between PRV and waste1 @23:52:39.77 CSV.dial :air @23:52:40.23 CS.seek 10ml @23:52:52.42 CSV.dial CTV @23:52:52.85 CS.seek 0ml @23:53:19.05 CBV.dial :waste1 @23:53:19.33 delay 5 seconds @23:53:24.35 CTV.dial :air,avoiding: :intake @23:53:25.65 Storing lyfil puck in Collection Clamp to tube 1 @23:53:25.81 SC.reconfigure :tight @23:53:25.85 SC.seek 1 @23:53:29.48 SC.configure :limp @23:53:29.68 Elbow.reconfigure :tight @23:53:29.72 Elbow.seek 1 @23:53:31.49 Forearm.reconfigure :tight @23:53:31.53 Forearm.seek 1 + 700 counts @23:53:32.56 SE.reconfigure :pick @23:53:32.61 SE.seek :beamBreak @23:53:34.40 SE.configure :default @23:53:34.55 Elbow.reconfigure :limp @23:53:34.59 Forearm.reconfigure :loose @23:53:34.63 Forearm.seek 1 @23:53:35.03 Elbow.hold @23:53:35.09 Elbow.reconfigure :tight @23:53:35.18 SE.seek 20.34 Pucks below Top Plate @23:53:35.55 Forearm.force -20 @23:53:35.59 Forearm.seek 1 + 320 counts @23:53:35.89 Forearm.reconfigure :tight @23:53:35.94 Forearm.seek 1 + 700 counts @23:53:36.24 SE.seek 21.34 Pucks below Top Plate @23:53:37.25 Forearm.reconfigure :loose @23:53:37.30 Forearm.seek :retract @23:53:38.22 Elbow.seek CC @23:53:39.81 Elbow.reconfigure :loose @23:53:39.90 Forearm.seek CC @23:53:40.92 Hand.close @23:53:41.58 CC.seek :open @23:53:46.01 Forearm.seek :retract @23:53:47.08 Elbow.reconfigure :tight @23:53:47.12 Elbow.seek 1 @23:53:48.90 Elbow.reconfigure :limp @23:53:48.95 Forearm.seek 1 @23:53:50.01 Elbow.hold @23:53:50.07 Elbow.reconfigure :tight @23:53:50.12 Hand.open @23:53:50.95 Forearm.force -20 @23:53:50.98 Forearm.seek 1 + 320 counts @23:53:51.33 Forearm.reconfigure :tight @23:53:51.38 Forearm.seek 1 + 700 counts @23:53:51.64 SE.seek :down @23:53:52.76
Forearm.reconfigure :loose @23:53:52.81 Forearm.seek :retract @23:53:52.91 SE.stop @23:53:53.78
PSV.dial :air @23:53:54.50 PS.seek 2.5ml @23:54:20.69 delay 1 second @23:54:21.71 PSV.dial :mixloop @23:54:22.36 delay 1 second @23:54:23.38 PSV.dial :air @23:54:23.82 delay 1 second @23:54:24.83 PSV.dial :mixloop @23:54:25.26 delay 1 second @23:54:26.27 PSV.dial :air @23:54:26.71 delay 1 second @23:54:27.73 PS.seek 0ml @23:54:53.91 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @23:54:54.08 Elbow.seek FlushPuck::Garage @23:54:55.82 Elbow.reconfigure :loose @23:54:55.90 Forearm.seek :garage @23:54:57.00 Hand.close @23:54:57.68 Forearm.seek :retract @23:54:58.86 Elbow.reconfigure :tight @23:54:58.90 Elbow.seek PC @23:55:00.21 Elbow.reconfigure :loose @23:55:00.29 Forearm.reconfigure :tight @23:55:00.34 Forearm.seek PC @23:55:01.67 PC.seek :closed @23:55:05.53 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.45 @23:55:05.60 Hand.open @23:55:06.38 Forearm.reconfigure :loose @23:55:06.42 Forearm.seek :retract @23:55:07.50 PRV.dial :air @23:55:07.92 PSV.dial :mixloop @23:55:08.42 PS.seek 0.3ml @23:55:12.25 PSV.dial PSR @23:55:13.27 Pulling 0.4ml of PSR.air @23:55:13.28 PSR.select :air @23:55:13.31 PS.seek 0.7ml @23:55:18.25 delay 3 seconds @23:55:21.27 PSR.select :air @23:55:21.29 PS.seek 2.5ml @23:55:40.31 delay 3 seconds @23:55:43.33 PSV.dial :mixloop @23:55:44.30 PTV.dial :mixing @23:55:44.99 PBV.dial :waste2 @23:55:45.85 PS.seek 0ml @23:56:12.10 Garaging FlushPuck in Processing Clamp @23:56:12.16 Elbow.reconfigure :tight @23:56:12.20 Elbow.seek PC @23:56:12.78 Elbow.reconfigure :loose @23:56:12.87 Forearm.seek PC @23:56:13.84 Hand.close @23:56:14.45 PC.seek :open @23:56:18.39 Forearm.seek :retract @23:56:19.37 Elbow.reconfigure :tight @23:56:19.41 Elbow.seek :garage @23:56:20.91 Elbow.reconfigure :loose @23:56:21.00 Forearm.reconfigure :tight @23:56:21.04 Forearm.seek :garage @23:56:22.21 Hand.open @23:56:23.01 Forearm.reconfigure :loose @23:56:23.05 Forearm.seek :retract @23:56:24.09 Loading da puck from tube 2 into Processing Clamp @23:56:24.31 SC.reconfigure :tight @23:56:24.35 SC.seek 2 @23:56:27.96 SC.configure :limp @23:56:28.14 Elbow.reconfigure :tight @23:56:28.19 Elbow.seek 2 @23:56:30.06 Forearm.reconfigure :tight @23:56:30.10 Forearm.seek 2 + 700 counts @23:56:31.15 SE.reconfigure :pick @23:56:31.20 SE.seek :beamBreak @23:56:33.54 SE.configure :default @23:56:33.67 Elbow.seek 2 @23:56:33.85 Forearm.seek 2 + 300 counts @23:56:34.01 Forearm.reconfigure :loose @23:56:34.05 Hand.close @23:56:34.71 SE.seek :down @23:56:34.90
Forearm.seek :retract @23:56:35.83 Elbow.seek PC @23:56:36.82 SE.stop @23:56:38.36
Elbow.reconfigure :loose @23:56:38.45 Forearm.reconfigure :tight @23:56:38.49 Forearm.seek PC @23:56:39.79 PC.seek :closed @23:56:43.66 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.39 @23:56:43.67 Hand.open @23:56:44.50 Forearm.reconfigure :loose @23:56:44.54 Forearm.seek :retract @23:56:45.58 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @23:56:45.60 PH.seek 28C,5:00 @23:57:35.88 PH.hold 2:00:00,28C @23:57:35.93 delay 1:30 @23:59:05.95 PSR.select :air @23:59:05.97 PSV.dial PSR @23:59:07.00 Pulling 0.5ml of PSR.wash @23:59:07.01 PSR.select :wash @23:59:07.13 PS.seek 0.5ml @23:59:12.97 delay 5 seconds @23:59:17.98 PSR.select :air @23:59:18.10 PS.seek 2ml @23:59:34.15 PTV.dial :puck @23:59:34.82 PSV.dial PTV @23:59:35.54 PBV.dial :waste1 @23:59:37.34 PS.seek 1.5ml @23:59:43.21 delay 3 seconds @23:59:46.23 PBV.dial between PSV and waste1 @23:59:46.58 PS.seek 0ml @00:00:02.65UTC10-Nov-22 delay 5:00 @00:05:02.68 recoverPSRreagent08 @00:05:02.69 PTV.dial :puck @00:05:02.70 PBV.dial :waste1 @00:05:03.05 delay 30 seconds @00:05:33.07 PBV.dial between PSV and waste1 @00:05:33.42 PSR.select :air @00:05:33.44 PSV.dial PSR @00:05:34.16 PS.reconfigure :fast @00:05:34.21 PS.seek 2.5ml @00:05:56.77 PSV.dial PTV @00:05:57.49 delay 5 seconds @00:06:02.50 PSV.dial PSR @00:06:03.22 delay 5 seconds @00:06:08.24 PSV.dial PTV @00:06:09.06 delay 5 seconds @00:06:14.08 PSV.dial :air @00:06:14.82 PS.seek 0ml @00:06:37.05 PTV.dial :PRVmixing,via: PRV @00:06:38.30 PSV.dial :mixloop @00:06:38.75 PRV.dial :ab1 @00:06:39.46 PS.reconfigure :slow @00:06:39.50 PS.seek 0.15ml @00:06:43.05 delay 2 seconds @00:06:45.06 CBV.dial PRV @00:06:45.09
PRV.dial CBV @00:06:45.64 PS.seek 0.34ml @00:06:50.05 delay 2 seconds @00:06:52.06 CBV.dial between PRV and PSV @00:06:52.37 PRV.dial :air @00:06:52.83 PS.seek 0.9ml @00:07:04.70 PRV.dial :waste2 @00:07:05.43 PS.seek 0.5ml @00:07:13.98 addPMLReagent08 @00:07:13.99 PSV.dial :air @00:07:14.44 PS.reconfigure :default @00:07:14.48 PS.seek 2ml @00:07:30.48 PSV.dial :PML @00:07:30.92 PTV.dial :mixing @00:07:31.62 PBV.dial :waste1 @00:07:32.03 PS.seek 1.85ml @00:07:34.42 PBV.dial between PSV and waste1 @00:07:34.79 PS.seek 0ml @00:07:54.32 delay 2 seconds @00:07:56.34 Beginning samplePassC @00:07:56.35 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:07:56.51 Elbow.reconfigure :tight @00:07:56.56 Elbow.seek FlushPuck::Garage @00:07:58.06 Elbow.reconfigure :loose @00:07:58.14 Forearm.seek :garage @00:07:59.27 Hand.close @00:08:00.03 Forearm.seek :retract @00:08:01.18 Elbow.reconfigure :tight @00:08:01.22 Elbow.seek CC @00:08:04.05 Elbow.reconfigure :loose @00:08:04.14 Forearm.reconfigure :tight @00:08:04.18 Forearm.seek CC @00:08:05.48 CC.seek :closed @00:08:09.71 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.86 @00:08:09.78 Hand.open @00:08:10.63 Forearm.reconfigure :loose @00:08:10.67 Forearm.seek :retract @00:08:11.67 PSV.dial PSR @00:08:12.71 Pulling 1.5ml of PSR.flush @00:08:12.72 PSR.select :flush @00:08:12.83 PS.seek 1.5ml @00:08:28.80 delay 5 seconds @00:08:33.81 PSV.dial :waste1 @00:08:34.25 PS.seek 0ml @00:08:50.32 PSV.dial PSR @00:08:50.87 PSR.select :air @00:08:50.98 PS.seek 2.5ml @00:09:17.92 PRV.dial CBV,avoiding: :ab2 @00:09:17.95
CBV.dial PRV @00:09:19.51 PSV.dial :mixloop @00:09:20.51 CTV.dial :air,avoiding: :intake @00:09:20.52 PTV.dial :PRVmixing @00:09:21.24 PS.seek 0ml @00:09:47.45 PSV.dial :air @00:09:47.87 PS.seek 2.5ml @00:10:14.04 PSV.dial :mixloop @00:10:14.48 PS.seek 0ml @00:10:40.66 PSV.dial :air @00:10:41.11 PS.seek 2.5ml @00:11:07.35 PSV.dial :mixloop @00:11:07.79 PS.seek 0ml @00:11:34.04 Garaging FlushPuck in Collection Clamp @00:11:34.11 Elbow.reconfigure :tight @00:11:34.15 Elbow.seek CC @00:11:34.25 Elbow.reconfigure :loose @00:11:34.34 Forearm.seek CC @00:11:35.29 Hand.close @00:11:35.92 CC.seek :open @00:11:40.25 Forearm.seek :retract @00:11:41.31 Elbow.reconfigure :tight @00:11:41.35 Elbow.seek :garage @00:11:44.24 Elbow.reconfigure :loose @00:11:44.32 Forearm.reconfigure :tight @00:11:44.37 Forearm.seek :garage @00:11:45.47 Hand.open @00:11:46.22 Forearm.reconfigure :loose @00:11:46.27 Forearm.seek :retract @00:11:47.31 delayUntil "00:15:56.33" @00:11:47.33 Whole Cell Archive sampling 1000ml @00:11:47.35 Loading wcr puck from tube 2 into Collection Clamp @00:11:47.79 Elbow.reconfigure :tight @00:11:47.83 Elbow.seek 2 @00:11:49.81 Forearm.reconfigure :tight @00:11:49.85 Forearm.seek 2 + 700 counts @00:11:50.93 SE.reconfigure :pick @00:11:50.98 SE.seek :beamBreak @00:11:53.49 SE.configure :default @00:11:53.63 Elbow.seek 2 @00:11:53.81 Forearm.seek 2 + 300 counts @00:11:54.07 Forearm.reconfigure :loose @00:11:54.12 Hand.close @00:11:54.68 SE.seek :down @00:11:54.87 Forearm.seek :retract @00:11:55.81 Elbow.seek CC @00:11:56.86 SE.stop @00:11:57.38 Elbow.reconfigure :loose @00:11:57.47 Forearm.reconfigure :tight @00:11:57.52 Forearm.seek CC @00:11:58.81 CC.seek :closed @00:12:03.05 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.74 @00:12:03.07 Hand.open @00:12:03.85 Forearm.reconfigure :loose @00:12:03.89 Forearm.seek :retract @00:12:04.89 collectSample 1000ml w/bubble point of 35psi @00:12:04.90 Priming 7ml @00:12:04.91 Intake.select :close @00:12:04.92 Exhaust.select :close @00:12:05.38 SS.hold @00:12:05.58 Calibrating pressure sensors @00:12:05.59 CTV.dial :air @00:12:05.61 SSV.dial :spit,avoiding: :exhaust @00:12:05.62 SS.reconfigure :fast @00:12:05.67 SS.seek 5ml @00:12:08.39 SSV.dial CBV @00:12:08.42 CBV.dial SSV @00:12:09.72 delay 3.8 seconds @00:12:13.64 SSV.dial :spit @00:12:14.94 SS.seek 0ml @00:12:17.95 SSV.dial CBV @00:12:19.20 CTV.dial :intake @00:12:19.93 SSV.dial CBV @00:12:19.95 CBV.dial SSV @00:12:19.97 Allowing 40s for 7.0ml of remaining 7.0ml @00:12:19.98 Intake.select :open @00:12:22.13 Intake @15.3psia, vacuum from 9.8 to 12.8psi @00:12:22.15 SS.reconfigure :inhaling 2.14ml/s @00:12:22.20 SS.seek 7ml @00:12:27.93 Stroke completed -- waiting for pressure to equalize ... @00:12:27.95 SS.reconfigure :exhale @00:12:27.99 SS.hold @00:12:36.45 Intake.select :close @00:12:36.59 SSV.dial :exhaust @00:12:37.31 Exhaust.select :open @00:12:37.42 SS.seek 0ml @00:12:41.77 Exhaust.select :close @00:12:41.88 Sampling 1000.0ml @00:12:41.89 Intake.select :close @00:12:41.90 Exhaust.select :close @00:12:41.91 SS.reconfigure :default @00:12:41.95 SS.hold @00:12:42.13 CTV.dial :intake @00:12:42.15 SSV.dial CBV @00:12:42.18 CBV.dial SSV @00:12:42.92 Allowing 130s for 25.0ml of remaining 1000.0ml @00:12:42.94 Intake.select :open @00:12:45.09 Intake @15.5psia, vacuum from 10.0 to 13.0psi @00:12:45.10 SS.reconfigure :inhaling 2.14ml/s @00:12:45.15 SS.seek 25ml @00:12:49.47 Vacuum too high -- waiting up to 40 seconds @00:12:49.48 SS.reconfigure :exhale @00:12:49.53 SS.hold @00:12:51.47 Intake @13.7psia, vacuum from 8.2 to 11.2psi @00:12:51.49 SS.reconfigure :inhaling 1.93ml/s @00:12:51.54 SS.seek 25ml @00:12:55.28 Vacuum too high -- waiting up to 40 seconds @00:12:55.30 SS.reconfigure :exhale @00:12:55.34 SS.hold @00:12:58.55 Intake @15.0psia, vacuum from 9.5 to 12.5psi @00:12:58.57 SS.reconfigure :inhaling 1.74ml/s @00:12:58.61 SS.seek 25ml @00:13:02.44 Vacuum too high -- waiting up to 40 seconds @00:13:02.45 SS.reconfigure :exhale @00:13:02.50 SS.hold @00:13:04.71 Intake @14.2psia, vacuum from 8.7 to 11.7psi @00:13:04.73 SS.reconfigure :inhaling 1.57ml/s @00:13:04.77 SS.seek 25ml @00:13:07.60 Vacuum too high -- waiting up to 40 seconds @00:13:07.62 SS.reconfigure :exhale @00:13:07.66 SS.hold @00:13:09.56 Intake @14.3psia, vacuum from 8.8 to 11.8psi @00:13:09.58 SS.reconfigure :inhaling 1.41ml/s @00:13:09.63 SS.seek 25ml @00:13:13.41 Vacuum too high -- waiting up to 40 seconds @00:13:13.42 SS.reconfigure :exhale @00:13:13.47 SS.hold @00:13:15.57 Intake @14.1psia, vacuum from 8.6 to 11.6psi @00:13:15.59 SS.reconfigure :inhaling 1.27ml/s @00:13:15.63 SS.seek 25ml @00:13:18.93 Stroke completed -- waiting for pressure to equalize ... @00:13:18.94 SS.reconfigure :exhale @00:13:18.99 SS.hold @00:13:21.48 Intake.select :close @00:13:21.62 SSV.dial :exhaust @00:13:22.34 Exhaust.select :open @00:13:22.45 SS.seek 0ml @00:13:31.60 Exhaust.select :close @00:13:31.71 SSV.dial CBV @00:13:31.73 CBV.dial SSV @00:13:32.47 Allowing 130s for 25.0ml of remaining 975.0ml @00:13:32.48 Intake.select :open @00:13:34.64 Intake @15.2psia, vacuum from 9.7 to 12.7psi @00:13:34.65 SS.reconfigure :inhaling 1.27ml/s @00:13:34.70 SS.seek 25ml @00:13:39.00 Vacuum too high -- waiting up to 40 seconds @00:13:39.02 SS.reconfigure :exhale @00:13:39.06 SS.hold @00:13:39.76 Intake @13.3psia, vacuum from 7.8 to 10.8psi @00:13:39.78 SS.reconfigure :inhaling 1.14ml/s @00:13:39.83 SS.seek 25ml @00:13:43.47 Vacuum too high -- waiting up to 40 seconds @00:13:43.48 SS.reconfigure :exhale @00:13:43.53 SS.hold @00:13:45.20 Intake @14.3psia, vacuum from 8.8 to 11.8psi @00:13:45.22 SS.reconfigure :inhaling 1.03ml/s @00:13:45.26 SS.seek 25ml @00:13:51.66 Vacuum too high -- waiting up to 40 seconds @00:13:51.67 SS.reconfigure :exhale @00:13:51.71 SS.hold @00:13:53.36 Intake @13.7psia, vacuum from 8.2 to 11.2psi @00:13:53.37 SS.reconfigure :inhaling 0.93ml/s @00:13:53.42 SS.seek 25ml @00:14:03.85 Vacuum too high -- waiting up to 40 seconds @00:14:03.86 SS.reconfigure :exhale @00:14:03.90 SS.hold @00:14:05.95 Intake @14.3psia, vacuum from 8.8 to 11.8psi @00:14:05.96 SS.reconfigure :inhaling 0.84ml/s @00:14:06.01 SS.seek 25ml @00:14:10.67 Stroke completed -- waiting for pressure to equalize ... @00:14:10.68 SS.reconfigure :exhale @00:14:10.72 SS.hold @00:14:12.67 Intake.select :close @00:14:12.81 SSV.dial :exhaust @00:14:13.54 Exhaust.select :open @00:14:13.65 SS.seek 0ml @00:14:22.63 Exhaust.select :close @00:14:22.74 SSV.dial CBV @00:14:22.77 CBV.dial SSV @00:14:23.53 Allowing 130s for 25.0ml of remaining 950.0ml @00:14:23.54 Intake.select :open @00:14:25.69 Intake @15.1psia, vacuum from 9.6 to 12.6psi @00:14:25.71 SS.reconfigure :inhaling 0.84ml/s @00:14:25.75 SS.seek 25ml @00:14:44.60 Vacuum too high -- waiting up to 40 seconds @00:14:44.61 SS.reconfigure :exhale @00:14:44.65 SS.hold @00:14:45.62 Intake @13.4psia, vacuum from 7.9 to 10.9psi @00:14:45.64 SS.reconfigure :inhaling 0.76ml/s @00:14:45.68 SS.seek 25ml @00:15:00.73 Stroke completed -- waiting for pressure to equalize ... @00:15:00.74 SS.reconfigure :exhale @00:15:00.78 SS.hold @00:15:03.68 Intake.select :close @00:15:03.83 SSV.dial :exhaust @00:15:04.56 Exhaust.select :open @00:15:04.67 SS.seek 0ml @00:15:13.55 Exhaust.select :close @00:15:13.66 SSV.dial CBV @00:15:13.69 CBV.dial SSV @00:15:14.43 Allowing 130s for 25.0ml of remaining 925.0ml @00:15:14.45 Intake.select :open @00:15:16.60 Intake @15.0psia, vacuum from 9.5 to 12.5psi @00:15:16.62 SS.reconfigure :inhaling 0.76ml/s @00:15:16.66 SS.seek 25ml @00:15:52.22 Stroke completed -- waiting for pressure to equalize ... @00:15:52.24 SS.reconfigure :exhale @00:15:52.28 SS.hold @00:15:55.73 Intake.select :close @00:15:55.87 SSV.dial :exhaust @00:15:56.34 recoverPRVreagent08 @00:15:56.35 PTV.dial :mixing @00:15:56.60 Exhaust.select :open @00:15:56.71 SS.seek 0ml @00:15:57.06 PBV.dial :waste1 @00:15:57.42 delay 30 seconds @00:16:06.04 Exhaust.select :close @00:16:06.15 SSV.dial CBV @00:16:06.17 CBV.dial SSV @00:16:06.93 Allowing 130s for 25.0ml of remaining 900.0ml @00:16:06.94 Intake.select :open @00:16:09.10 Intake @15.0psia, vacuum from 9.5 to 12.5psi @00:16:09.11 SS.reconfigure :inhaling 0.76ml/s @00:16:09.16 SS.seek 25ml @00:16:27.44 PBV.dial between PSV and waste1 @00:16:27.81 PSV.dial :air @00:16:28.27 PS.reconfigure :fast @00:16:28.31 PS.seek 2.5ml @00:16:35.03 Vacuum too high -- waiting up to 40 seconds @00:16:35.04 SS.reconfigure :exhale @00:16:35.08 SS.hold @00:16:36.37 Intake @13.1psia, vacuum from 7.6 to 10.6psi @00:16:36.39 SS.reconfigure :inhaling 0.69ml/s @00:16:36.43 SS.seek 25ml @00:16:46.61 Stroke completed -- waiting for pressure to equalize ... @00:16:46.63 SS.reconfigure :exhale @00:16:46.67 SS.hold @00:16:49.66 Intake.select :close @00:16:49.80 SSV.dial :exhaust @00:16:50.54 Exhaust.select :open @00:16:50.65 SS.seek 0ml @00:16:51.03 PSV.dial :mixloop @00:16:51.47 delay 2 seconds @00:16:53.48 PSV.dial :air @00:16:53.94 delay 2 seconds @00:16:55.95 PSV.dial :mixloop @00:16:56.40 delay 2 seconds @00:16:58.42 PSV.dial :air @00:16:58.88 PS.seek 0ml @00:17:00.15 Exhaust.select :close @00:17:00.25 SSV.dial CBV @00:17:00.28 CBV.dial SSV @00:17:01.02 Allowing 130s for 25.0ml of remaining 875.0ml @00:17:01.04 Intake.select :open @00:17:03.19 Intake @15.0psia, vacuum from 9.5 to 12.5psi @00:17:03.21 SS.reconfigure :inhaling 0.69ml/s @00:17:03.25 SS.seek 25ml @00:17:21.44 washCycleDA #1 of 2 @00:17:21.45 PSR.select :air @00:17:21.92 PSV.dial PSR @00:17:23.24 Pulling 0.5ml of PSR.wash @00:17:23.25 PSR.select :wash @00:17:23.36 PS.reconfigure :default @00:17:23.40 PS.seek 0.5ml @00:17:29.34 delay 5 seconds @00:17:34.36 PSR.select :air @00:17:34.47 PS.seek 2ml @00:17:41.63 Stroke completed -- waiting for pressure to equalize ... @00:17:41.64 SS.reconfigure :exhale @00:17:41.68 SS.hold @00:17:45.82 Intake.select :close @00:17:45.96 SSV.dial :exhaust @00:17:46.70 Exhaust.select :open @00:17:46.81 SS.seek 0ml @00:17:50.43 PTV.dial :puck @00:17:51.15 PSV.dial PTV @00:17:51.98 PBV.dial :waste1 @00:17:52.40 PS.seek 1.5ml @00:17:55.83 Exhaust.select :close @00:17:55.94 SSV.dial CBV @00:17:55.97 CBV.dial SSV @00:17:56.71 Allowing 130s for 25.0ml of remaining 850.0ml @00:17:56.72 Intake.select :open @00:17:58.34 delay 3 seconds @00:17:58.87 Intake @14.9psia, vacuum from 9.4 to 12.4psi @00:17:58.89 SS.reconfigure :inhaling 0.69ml/s @00:17:58.93 SS.seek 25ml @00:18:01.36 PBV.dial between PSV and waste1 @00:18:01.72 PS.seek 0ml @00:18:17.78 delay 2:00 @00:18:31.59 Vacuum too high -- waiting up to 40 seconds @00:18:31.60 SS.reconfigure :exhale @00:18:31.64 SS.hold @00:18:33.94 Intake @14.3psia, vacuum from 8.8 to 11.8psi @00:18:33.96 SS.reconfigure :inhaling 0.61ml/s @00:18:34.00 SS.seek 25ml @00:18:42.02 Stroke completed -- waiting for pressure to equalize ... @00:18:42.03 SS.reconfigure :exhale @00:18:42.07 SS.hold @00:18:45.00 Intake.select :close @00:18:45.14 SSV.dial :exhaust @00:18:45.88 Exhaust.select :open @00:18:45.99 SS.seek 0ml @00:18:54.88 Exhaust.select :close @00:18:54.98 SSV.dial CBV @00:18:55.01 CBV.dial SSV @00:18:55.75 Allowing 130s for 25.0ml of remaining 825.0ml @00:18:55.77 Intake.select :open @00:18:57.92 Intake @15.1psia, vacuum from 9.6 to 12.6psi @00:18:57.94 SS.reconfigure :inhaling 0.61ml/s @00:18:57.99 SS.seek 25ml @00:19:41.56 Stroke completed -- waiting for pressure to equalize ... @00:19:41.57 SS.reconfigure :exhale @00:19:41.61 SS.hold @00:19:45.77 Intake.select :close @00:19:45.91 SSV.dial :exhaust @00:19:46.64 Exhaust.select :open @00:19:46.75 SS.seek 0ml @00:19:56.03 Exhaust.select :close @00:19:56.14 SSV.dial CBV @00:19:56.17 CBV.dial SSV @00:19:56.89 Allowing 130s for 25.0ml of remaining 800.0ml @00:19:56.90 Intake.select :open @00:19:59.06 Intake @15.0psia, vacuum from 9.5 to 12.5psi @00:19:59.07 SS.reconfigure :inhaling 0.61ml/s @00:19:59.12 SS.seek 25ml @00:20:17.80 recoverPSRreagent08 @00:20:18.27 PTV.dial :puck @00:20:18.30 PBV.dial :waste1 @00:20:18.65 delay 30 seconds @00:20:41.81 Stroke completed -- waiting for pressure to equalize ... @00:20:41.82 SS.reconfigure :exhale @00:20:41.87 SS.hold @00:20:46.63 Intake.select :close @00:20:46.77 SSV.dial :exhaust @00:20:47.51 Exhaust.select :open @00:20:47.62 SS.seek 0ml @00:20:48.67 PBV.dial between PSV and waste1 @00:20:49.02 PSR.select :air @00:20:49.04 PSV.dial PSR @00:20:49.75 PS.reconfigure :fast @00:20:49.79 PS.seek 2.5ml @00:20:56.95 Exhaust.select :close @00:20:57.06 SSV.dial CBV @00:20:57.10 CBV.dial SSV @00:20:57.81 Allowing 130s for 25.0ml of remaining 775.0ml @00:20:57.82 Intake.select :open @00:20:59.98 Intake @15.0psia, vacuum from 9.5 to 12.5psi @00:21:00.00 SS.reconfigure :inhaling 0.61ml/s @00:21:00.04 SS.seek 25ml @00:21:12.49 PSV.dial PTV @00:21:13.21 delay 5 seconds @00:21:18.23 PSV.dial PSR @00:21:18.94 delay 5 seconds @00:21:21.46 Vacuum too high -- waiting up to 40 seconds @00:21:21.47 SS.reconfigure :exhale @00:21:21.51 SS.hold @00:21:22.80 Intake @13.6psia, vacuum from 8.1 to 11.1psi @00:21:22.82 SS.reconfigure :inhaling 0.56ml/s @00:21:22.86 SS.seek 25ml @00:21:23.96 PSV.dial PTV @00:21:24.68 delay 5 seconds @00:21:29.70 PSV.dial :air @00:21:30.42 PS.seek 0ml @00:21:47.42 Stroke completed -- waiting for pressure to equalize ... @00:21:47.43 SS.reconfigure :exhale @00:21:47.47 SS.hold @00:21:51.54 Intake.select :close @00:21:51.68 SSV.dial :exhaust @00:21:52.43 Exhaust.select :open @00:21:52.54 SS.seek 0ml @00:21:52.86 washCycleDA #2 of 2 @00:21:52.88 PSR.select :air @00:21:52.89 PSV.dial PSR @00:21:54.20 Pulling 0.5ml of PSR.wash @00:21:54.21 PSR.select :wash @00:21:54.32 PS.reconfigure :default @00:21:54.36 PS.seek 0.5ml @00:22:00.24 delay 5 seconds @00:22:01.84 Exhaust.select :close @00:22:01.95 SSV.dial CBV @00:22:01.97 CBV.dial SSV @00:22:02.72 Allowing 130s for 25.0ml of remaining 750.0ml @00:22:02.73 Intake.select :open @00:22:04.88 Intake @15.0psia, vacuum from 9.5 to 12.5psi @00:22:04.90 SS.reconfigure :inhaling 0.56ml/s @00:22:04.94 SS.seek 25ml @00:22:05.26 PSR.select :air @00:22:05.37 PS.seek 2ml @00:22:21.46 PTV.dial :puck @00:22:21.47 PSV.dial PTV @00:22:22.16 PBV.dial :waste1 @00:22:22.56 PS.seek 1.5ml @00:22:28.49 delay 3 seconds @00:22:31.51 PBV.dial between PSV and waste1 @00:22:31.87 PS.seek 0ml @00:22:47.90 delay 2:00 @00:22:53.72 Stroke completed -- waiting for pressure to equalize ... @00:22:53.73 SS.reconfigure :exhale @00:22:53.77 SS.hold @00:22:59.01 Intake.select :close @00:22:59.16 SSV.dial :exhaust @00:22:59.89 Exhaust.select :open @00:23:00.00 SS.seek 0ml @00:23:08.99 Exhaust.select :close @00:23:09.10 SSV.dial CBV @00:23:09.14 CBV.dial SSV @00:23:09.86 Allowing 130s for 25.0ml of remaining 725.0ml @00:23:09.87 Intake.select :open @00:23:12.02 Intake @15.0psia, vacuum from 9.5 to 12.5psi @00:23:12.04 SS.reconfigure :inhaling 0.56ml/s @00:23:12.09 SS.seek 25ml @00:24:00.58 Stroke completed -- waiting for pressure to equalize ... @00:24:00.60 SS.reconfigure :exhale @00:24:00.64 SS.hold @00:24:07.13 Intake.select :close @00:24:07.27 SSV.dial :exhaust @00:24:08.01 Exhaust.select :open @00:24:08.12 SS.seek 0ml @00:24:17.13 Exhaust.select :close @00:24:17.24 SSV.dial CBV @00:24:17.26 CBV.dial SSV @00:24:18.01 Allowing 130s for 25.0ml of remaining 700.0ml @00:24:18.02 Intake.select :open @00:24:20.17 Intake @15.1psia, vacuum from 9.6 to 12.6psi @00:24:20.19 SS.reconfigure :inhaling 0.56ml/s @00:24:20.24 SS.seek 25ml @00:24:47.92 recoverPSRreagent08 @00:24:47.93 PTV.dial :puck @00:24:47.94 PBV.dial :waste1 @00:24:48.28 delay 30 seconds @00:25:03.42 Vacuum too high -- waiting up to 40 seconds @00:25:03.43 SS.reconfigure :exhale @00:25:03.47 SS.hold @00:25:05.96 Intake @13.4psia, vacuum from 7.9 to 10.9psi @00:25:05.98 SS.reconfigure :inhaling 0.50ml/s @00:25:06.02 SS.seek 25ml @00:25:13.19 Stroke completed -- waiting for pressure to equalize ... @00:25:13.20 SS.reconfigure :exhale @00:25:13.25 SS.hold @00:25:18.10 Intake.select :close @00:25:18.25 SSV.dial :exhaust @00:25:18.30 PBV.dial between PSV and waste1 @00:25:18.66 PSR.select :air @00:25:18.67 PSV.dial PSR @00:25:18.97 Exhaust.select :open @00:25:19.08 SS.seek 0ml @00:25:19.40 PS.reconfigure :fast @00:25:19.44 PS.seek 2.5ml @00:25:28.39 Exhaust.select :close @00:25:28.50 SSV.dial CBV @00:25:28.53 CBV.dial SSV @00:25:29.27 Allowing 130s for 25.0ml of remaining 675.0ml @00:25:29.28 Intake.select :open @00:25:31.44 Intake @15.1psia, vacuum from 9.6 to 12.6psi @00:25:31.45 SS.reconfigure :inhaling 0.50ml/s @00:25:31.50 SS.seek 25ml @00:25:42.17 PSV.dial PTV @00:25:42.89 delay 5 seconds @00:25:47.91 PSV.dial PSR @00:25:48.62 delay 5 seconds @00:25:53.64 PSV.dial PTV @00:25:54.36 delay 5 seconds @00:25:59.38 PSV.dial :air @00:26:00.10 PS.seek 0ml @00:26:22.64 getPMLReagent08 @00:26:22.65 PRV.dial :air @00:26:23.08 delay 5 seconds @00:26:27.06 Stroke completed -- waiting for pressure to equalize ... @00:26:27.08 SS.reconfigure :exhale @00:26:27.60 SS.hold @00:26:28.09 PTV.dial :PRVmixing @00:26:29.33 delay 5 seconds @00:26:32.51 Intake.select :close @00:26:32.65 SSV.dial :exhaust @00:26:33.39 Exhaust.select :open @00:26:33.50 SS.seek 0ml @00:26:34.34 PRV.dial :ab2 @00:26:34.79 PTV.dial :PRVmixing @00:26:34.81 PSV.dial :mixloop @00:26:35.25 PS.reconfigure :default @00:26:35.30 PS.seek 0.3ml @00:26:39.11 delay 2 seconds @00:26:41.12 PRV.dial :air @00:26:41.63 PS.seek 0.55ml @00:26:42.86 Exhaust.select :close @00:26:42.97 SSV.dial CBV @00:26:42.99 CBV.dial SSV @00:26:43.74 Allowing 130s for 25.0ml of remaining 650.0ml @00:26:43.75 Intake.select :open @00:26:44.98 addPMLReagent08 @00:26:44.99 PSV.dial :air @00:26:45.44 PS.seek 2ml @00:26:45.90 Intake @15.1psia, vacuum from 9.6 to 12.6psi @00:26:45.92 SS.reconfigure :inhaling 0.50ml/s @00:26:45.97 SS.seek 25ml @00:27:01.01 PSV.dial :PML @00:27:01.45 PTV.dial :mixing @00:27:02.17 PBV.dial :waste1 @00:27:02.57 PS.seek 1.85ml @00:27:04.98 PBV.dial between PSV and waste1 @00:27:05.35 PS.seek 0ml @00:27:24.98 delay 2 seconds @00:27:27.00 delay 8:00 @00:27:41.11 Stroke completed -- waiting for pressure to equalize ... @00:27:41.12 SS.reconfigure :exhale @00:27:41.16 SS.hold @00:27:48.53 Intake.select :close @00:27:48.68 SSV.dial :exhaust @00:27:49.41 Exhaust.select :open @00:27:49.52 SS.seek 0ml @00:27:58.58 Exhaust.select :close @00:27:58.68 SSV.dial CBV @00:27:58.71 CBV.dial SSV @00:27:59.45 Allowing 130s for 25.0ml of remaining 625.0ml @00:27:59.47 Intake.select :open @00:28:01.63 Intake @15.2psia, vacuum from 9.7 to 12.7psi @00:28:01.64 SS.reconfigure :inhaling 0.50ml/s @00:28:01.69 SS.seek 25ml @00:28:45.79 Vacuum too high -- waiting up to 40 seconds @00:28:45.80 SS.reconfigure :exhale @00:28:45.84 SS.hold @00:28:50.00 Intake @14.7psia, vacuum from 9.2 to 12.2psi @00:28:50.02 SS.reconfigure :inhaling 0.46ml/s @00:28:50.06 SS.seek 25ml @00:29:02.70 Stroke completed -- waiting for pressure to equalize ... @00:29:02.71 SS.reconfigure :exhale @00:29:02.75 SS.hold @00:29:08.38 Intake.select :close @00:29:08.52 SSV.dial :exhaust @00:29:09.25 Exhaust.select :open @00:29:09.36 SS.seek 0ml @00:29:18.39 Exhaust.select :close @00:29:18.49 SSV.dial CBV @00:29:18.52 CBV.dial SSV @00:29:19.28 Allowing 130s for 25.0ml of remaining 600.0ml @00:29:19.29 Intake.select :open @00:29:21.45 Intake @15.1psia, vacuum from 9.6 to 12.6psi @00:29:21.47 SS.reconfigure :inhaling 0.46ml/s @00:29:21.52 SS.seek 25ml @00:30:23.43 Stroke completed -- waiting for pressure to equalize ... @00:30:23.44 SS.reconfigure :exhale @00:30:23.48 SS.hold @00:30:31.95 Intake.select :close @00:30:32.09 SSV.dial :exhaust @00:30:32.82 Exhaust.select :open @00:30:32.94 SS.seek 0ml @00:30:42.32 Exhaust.select :close @00:30:42.43 SSV.dial CBV @00:30:42.46 CBV.dial SSV @00:30:43.18 Allowing 130s for 25.0ml of remaining 575.0ml @00:30:43.20 Intake.select :open @00:30:45.35 Intake @15.1psia, vacuum from 9.6 to 12.6psi @00:30:45.37 SS.reconfigure :inhaling 0.46ml/s @00:30:45.41 SS.seek 25ml @00:31:30.19 Vacuum too high -- waiting up to 40 seconds @00:31:30.20 SS.reconfigure :exhale @00:31:30.24 SS.hold @00:31:33.78 Intake @14.2psia, vacuum from 8.7 to 11.7psi @00:31:33.80 SS.reconfigure :inhaling 0.41ml/s @00:31:33.84 SS.seek 25ml @00:31:51.65 Stroke completed -- waiting for pressure to equalize ... @00:31:51.66 SS.reconfigure :exhale @00:31:51.70 SS.hold @00:31:59.06 Intake.select :close @00:31:59.20 SSV.dial :exhaust @00:31:59.93 Exhaust.select :open @00:32:00.04 SS.seek 0ml @00:32:09.44 Exhaust.select :close @00:32:09.55 SSV.dial CBV @00:32:09.59 CBV.dial SSV @00:32:10.32 Allowing 130s for 25.0ml of remaining 550.0ml @00:32:10.33 Intake.select :open @00:32:12.49 Intake @15.2psia, vacuum from 9.7 to 12.7psi @00:32:12.50 SS.reconfigure :inhaling 0.41ml/s @00:32:12.55 SS.seek 25ml @00:33:20.63 Stroke completed -- waiting for pressure to equalize ... @00:33:20.64 SS.reconfigure :exhale @00:33:20.68 SS.hold @00:33:30.45 Intake.select :close @00:33:30.60 SSV.dial :exhaust @00:33:31.34 Exhaust.select :open @00:33:31.45 SS.seek 0ml @00:33:40.49 Exhaust.select :close @00:33:40.60 SSV.dial CBV @00:33:40.62 CBV.dial SSV @00:33:41.37 Allowing 130s for 25.0ml of remaining 525.0ml @00:33:41.38 Intake.select :open @00:33:43.53 Intake @15.2psia, vacuum from 9.7 to 12.7psi @00:33:43.55 SS.reconfigure :inhaling 0.41ml/s @00:33:43.59 SS.seek 25ml @00:34:28.46 Vacuum too high -- waiting up to 40 seconds @00:34:28.47 SS.reconfigure :exhale @00:34:28.52 SS.hold @00:34:32.72 Intake @15.0psia, vacuum from 9.5 to 12.5psi @00:34:32.74 SS.reconfigure :inhaling 0.37ml/s @00:34:32.78 SS.seek 25ml @00:34:58.83 Stroke completed -- waiting for pressure to equalize ... @00:34:58.84 SS.reconfigure :exhale @00:34:58.88 SS.hold @00:35:06.33 Intake.select :close @00:35:06.47 SSV.dial :exhaust @00:35:07.19 Exhaust.select :open @00:35:07.30 SS.seek 0ml @00:35:16.33 Exhaust.select :close @00:35:16.44 SSV.dial CBV @00:35:16.46 CBV.dial SSV @00:35:17.19 Allowing 130s for 25.0ml of remaining 500.0ml @00:35:17.20 Intake.select :open @00:35:19.35 Intake @15.3psia, vacuum from 9.8 to 12.8psi @00:35:19.37 SS.reconfigure :inhaling 0.37ml/s @00:35:19.42 SS.seek 25ml @00:35:27.01 recoverPRVreagent08 @00:35:27.02 PTV.dial :mixing @00:35:27.03 PBV.dial :waste1 @00:35:27.39 delay 30 seconds @00:35:57.41 PBV.dial between PSV and waste1 @00:35:57.76 PSV.dial :air @00:35:58.22 PS.reconfigure :fast @00:35:58.26 PS.seek 2.5ml @00:36:21.03 PSV.dial :mixloop @00:36:21.59 delay 2 seconds @00:36:23.61 PSV.dial :air @00:36:24.20 delay 2 seconds @00:36:26.22 PSV.dial :mixloop @00:36:26.67 delay 2 seconds @00:36:28.68 PSV.dial :air @00:36:29.14 PS.seek 0ml @00:36:36.93 Stroke completed -- waiting for pressure to equalize ... @00:36:36.95 SS.reconfigure :exhale @00:36:36.99 SS.hold @00:36:46.66 Intake.select :close @00:36:46.81 SSV.dial :exhaust @00:36:47.53 Exhaust.select :open @00:36:47.64 SS.seek 0ml @00:36:51.62 washCycleDA #1 of 3 @00:36:51.63 PSR.select :air @00:36:51.65 PSV.dial PSR @00:36:52.95 Pulling 0.5ml of PSR.wash @00:36:52.96 PSR.select :wash @00:36:53.08 PS.reconfigure :default @00:36:53.62 PS.seek 0.5ml @00:36:56.92 Exhaust.select :close @00:36:57.03 SSV.dial CBV @00:36:57.06 CBV.dial SSV @00:36:57.78 Allowing 130s for 25.0ml of remaining 475.0ml @00:36:57.79 Intake.select :open @00:36:59.59 delay 5 seconds @00:36:59.95 Intake @15.2psia, vacuum from 9.7 to 12.7psi @00:36:59.97 SS.reconfigure :inhaling 0.37ml/s @00:37:00.01 SS.seek 25ml @00:37:04.61 PSR.select :air @00:37:04.73 PS.seek 2ml @00:37:20.74 PTV.dial :puck @00:37:21.43 PSV.dial PTV @00:37:22.26 PBV.dial :waste1 @00:37:22.68 PS.seek 1.5ml @00:37:28.65 delay 3 seconds @00:37:31.67 PBV.dial between PSV and waste1 @00:37:32.04 PS.seek 0ml @00:37:48.09 delay 2:00 @00:38:16.71 Stroke completed -- waiting for pressure to equalize ... @00:38:16.72 SS.reconfigure :exhale @00:38:16.77 SS.hold @00:38:29.41 Intake.select :close @00:38:29.55 SSV.dial :exhaust @00:38:30.28 Exhaust.select :open @00:38:30.39 SS.seek 0ml @00:38:39.45 Exhaust.select :close @00:38:39.55 SSV.dial CBV @00:38:39.58 CBV.dial SSV @00:38:40.34 Allowing 130s for 25.0ml of remaining 450.0ml @00:38:40.35 Intake.select :open @00:38:42.51 Intake @15.2psia, vacuum from 9.7 to 12.7psi @00:38:42.52 SS.reconfigure :inhaling 0.37ml/s @00:38:42.57 SS.seek 25ml @00:39:26.77 Vacuum too high -- waiting up to 40 seconds @00:39:26.78 SS.reconfigure :exhale @00:39:26.82 SS.hold @00:39:30.98 Intake @14.3psia, vacuum from 8.8 to 11.8psi @00:39:31.00 SS.reconfigure :inhaling 0.33ml/s @00:39:31.04 SS.seek 25ml @00:39:48.11 recoverPSRreagent08 @00:39:48.12 PTV.dial :puck @00:39:48.13 PBV.dial :waste1 @00:39:48.49 delay 30 seconds @00:40:08.40 Stroke completed -- waiting for pressure to equalize ... @00:40:08.41 SS.reconfigure :exhale @00:40:08.45 SS.hold @00:40:18.51 PBV.dial between PSV and waste1 @00:40:18.87 PSR.select :air @00:40:18.89 PSV.dial PSR @00:40:19.47 Intake.select :close @00:40:19.59 PS.reconfigure :fast @00:40:19.63 PS.seek 2.5ml @00:40:19.72 SSV.dial :exhaust @00:40:20.46 Exhaust.select :open @00:40:20.57 SS.seek 0ml @00:40:29.97 Exhaust.select :close @00:40:30.08 SSV.dial CBV @00:40:30.52 CBV.dial SSV @00:40:31.35 Allowing 130s for 25.0ml of remaining 425.0ml @00:40:31.36 Intake.select :open @00:40:33.52 Intake @15.4psia, vacuum from 9.9 to 12.9psi @00:40:33.54 SS.reconfigure :inhaling 0.33ml/s @00:40:33.58 SS.seek 25ml @00:40:42.49 PSV.dial PTV @00:40:43.21 delay 5 seconds @00:40:48.23 PSV.dial PSR @00:40:48.94 delay 5 seconds @00:40:53.96 PSV.dial PTV @00:40:54.68 delay 5 seconds @00:40:59.70 PSV.dial :air @00:41:00.42 PS.seek 0ml @00:41:22.86 washCycleDA #2 of 3 @00:41:22.87 PSR.select :air @00:41:22.89 PSV.dial PSR @00:41:24.18 Pulling 0.5ml of PSR.wash @00:41:24.19 PSR.select :wash @00:41:24.30 PS.reconfigure :default @00:41:24.34 PS.seek 0.5ml @00:41:30.49 delay 5 seconds @00:41:35.51 PSR.select :air @00:41:35.62 PS.seek 2ml @00:41:51.61 PTV.dial :puck @00:41:51.62 PSV.dial PTV @00:41:52.44 PBV.dial :waste1 @00:41:52.86 PS.seek 1.5ml @00:41:58.86 delay 3 seconds @00:42:00.72 Stroke completed -- waiting for pressure to equalize ... @00:42:00.73 SS.reconfigure :exhale @00:42:00.77 SS.hold @00:42:01.91 PBV.dial between PSV and waste1 @00:42:02.29 PS.seek 0ml @00:42:13.33 Intake.select :close @00:42:13.48 SSV.dial :exhaust @00:42:14.21 Exhaust.select :open @00:42:14.32 SS.seek 0ml @00:42:18.33 delay 2:00 @00:42:23.31 Exhaust.select :close @00:42:23.42 SSV.dial CBV @00:42:23.45 CBV.dial SSV @00:42:24.21 Allowing 130s for 25.0ml of remaining 400.0ml @00:42:24.22 Intake.select :open @00:42:26.37 Intake @15.4psia, vacuum from 9.9 to 12.9psi @00:42:26.39 SS.reconfigure :inhaling 0.33ml/s @00:42:26.43 SS.seek 25ml @00:43:43.35 Vacuum too high -- waiting up to 40 seconds @00:43:43.36 SS.reconfigure :exhale @00:43:43.41 SS.hold @00:43:50.86 Intake @15.2psia, vacuum from 9.7 to 12.7psi @00:43:50.88 SS.reconfigure :inhaling 0.30ml/s @00:43:50.92 SS.seek 25ml @00:44:04.45 Stroke completed -- waiting for pressure to equalize ... @00:44:04.46 SS.reconfigure :exhale @00:44:04.50 SS.hold @00:44:14.29 Intake.select :close @00:44:14.43 SSV.dial :exhaust @00:44:15.16 Exhaust.select :open @00:44:15.77 SS.seek 0ml @00:44:18.35 recoverPSRreagent08 @00:44:18.36 PTV.dial :puck @00:44:18.37 PBV.dial :waste1 @00:44:18.72 delay 30 seconds @00:44:25.20 Exhaust.select :close @00:44:25.31 SSV.dial CBV @00:44:25.35 CBV.dial SSV @00:44:26.06 Allowing 130s for 25.0ml of remaining 375.0ml @00:44:26.08 Intake.select :open @00:44:28.24 Intake @15.5psia, vacuum from 10.0 to 13.0psi @00:44:28.25 SS.reconfigure :inhaling 0.30ml/s @00:44:28.30 SS.seek 25ml @00:44:48.74 PBV.dial between PSV and waste1 @00:44:49.09 PSR.select :air @00:44:49.11 PSV.dial PSR @00:44:49.82 PS.reconfigure :fast @00:44:49.86 PS.seek 2.5ml @00:45:12.51 PSV.dial PTV @00:45:13.23 delay 5 seconds @00:45:18.25 PSV.dial PSR @00:45:18.97 delay 5 seconds @00:45:23.99 PSV.dial PTV @00:45:24.70 delay 5 seconds @00:45:29.72 PSV.dial :air @00:45:30.44 PS.seek 0ml @00:45:52.92 washCycleDA #3 of 3 @00:45:52.93 PSR.select :air @00:45:52.95 PSV.dial PSR @00:45:54.24 Pulling 0.5ml of PSR.wash @00:45:54.25 PSR.select :wash @00:45:54.36 PS.reconfigure :default @00:45:54.40 PS.seek 0.5ml @00:46:00.36 delay 5 seconds @00:46:04.62 Stroke completed -- waiting for pressure to equalize ... @00:46:04.64 SS.reconfigure :exhale @00:46:04.68 SS.hold @00:46:05.37 PSR.select :air @00:46:05.53 PS.seek 2ml @00:46:20.88 Intake.select :close @00:46:21.03 SSV.dial :exhaust @00:46:21.60 PTV.dial :puck @00:46:21.62 PSV.dial PTV @00:46:21.76 Exhaust.select :open @00:46:21.87 SS.seek 0ml @00:46:22.32 PBV.dial :waste1 @00:46:22.74 PS.seek 1.5ml @00:46:28.70 delay 3 seconds @00:46:30.90 Exhaust.select :close @00:46:31.01 SSV.dial CBV @00:46:31.04 CBV.dial SSV @00:46:31.71 PBV.dial between PSV and waste1 @00:46:31.78 Allowing 130s for 25.0ml of remaining 350.0ml @00:46:31.79 Intake.select :open @00:46:32.08 PS.seek 0ml @00:46:33.94 Intake @15.5psia, vacuum from 10.0 to 13.0psi @00:46:33.96 SS.reconfigure :inhaling 0.30ml/s @00:46:34.01 SS.seek 25ml @00:46:48.29 delay 2:00 @00:48:00.94 Vacuum too high -- waiting up to 40 seconds @00:48:00.95 SS.reconfigure :exhale @00:48:00.99 SS.hold @00:48:09.54 Intake @14.5psia, vacuum from 9.0 to 12.0psi @00:48:09.56 SS.reconfigure :inhaling 0.27ml/s @00:48:09.60 SS.seek 25ml @00:48:21.60 Stroke completed -- waiting for pressure to equalize ... @00:48:21.61 SS.reconfigure :exhale @00:48:21.65 SS.hold @00:48:33.30 Intake.select :close @00:48:33.44 SSV.dial :exhaust @00:48:34.18 Exhaust.select :open @00:48:34.29 SS.seek 0ml @00:48:43.75 Exhaust.select :close @00:48:43.86 SSV.dial CBV @00:48:43.89 CBV.dial SSV @00:48:44.63 Allowing 130s for 25.0ml of remaining 325.0ml @00:48:44.64 Intake.select :open @00:48:46.80 Intake @15.3psia, vacuum from 9.8 to 12.8psi @00:48:46.82 SS.reconfigure :inhaling 0.27ml/s @00:48:46.86 SS.seek 25ml @00:48:48.31 recoverPSRreagent08 @00:48:48.32 PTV.dial :puck @00:48:48.33 PBV.dial :waste1 @00:48:48.68 delay 30 seconds @00:49:18.70 PBV.dial between PSV and waste1 @00:49:19.05 PSR.select :air @00:49:19.07 PSV.dial PSR @00:49:19.79 PS.reconfigure :fast @00:49:19.84 PS.seek 2.5ml @00:49:42.41 PSV.dial PTV @00:49:43.11 delay 5 seconds @00:49:48.13 PSV.dial PSR @00:49:48.85 delay 5 seconds @00:49:53.87 PSV.dial PTV @00:49:54.58 delay 5 seconds @00:49:59.60 PSV.dial :air @00:50:00.32 PS.seek 0ml @00:50:22.78 Gate.power :camera,:ON @00:50:22.80 Mixing DA Substrates @00:50:22.81 PSR.select :air @00:50:22.82 PSV.dial PSR @00:50:24.38 Pulling 0.25ml of PSR.sub1 @00:50:24.43 PSR.select :sub1 @00:50:24.73 PS.reconfigure :default @00:50:24.90 PS.seek 0.25ml @00:50:28.42 delay 5 seconds @00:50:33.49 Pulling 0.25ml of PSR.sub2 @00:50:33.50 PSR.select :sub2 @00:50:33.62 PS.seek 0.5ml @00:50:34.61 Stroke completed -- waiting for pressure to equalize ... @00:50:34.62 SS.reconfigure :exhale @00:50:34.66 SS.hold @00:50:37.04 delay 5 seconds @00:50:42.06 PSR.select :air @00:50:42.17 PS.seek 2ml @00:50:54.66 SS.reconfigure :default @00:50:54.70 SS.hold @00:50:54.81 Intake.select :close @00:50:54.95 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 700.0ml @00:50:54.97 SSV.dial :exhaust @00:50:55.69 Exhaust.select :open @00:50:55.80 SS.reconfigure :exhale @00:50:55.85 SS.seek 0ml @00:50:58.22 PTV.dial :puck @00:50:58.23 PSV.dial PTV @00:50:58.94 PBV.dial :waste1 @00:50:59.37 PS.seek 1.5ml @00:51:04.89 Exhaust.select :close @00:51:05.00 Sampled 700.0ml @00:51:05.02 Drying puck before fixing for 20:00 @00:51:05.03 CBV.dial :waste2b @00:51:05.28 delay 3 seconds @00:51:05.74 delay 1:00 @00:51:08.30 PBV.dial between PSV and waste1 @00:51:08.67 PS.seek 0ml @00:51:24.69 delay 10 seconds @00:51:34.72 recoverPSRreagent08 PTV.dial :puck @00:51:34.73 PBV.dial :waste1 @00:51:35.08 delay 30 seconds @00:52:05.10 PBV.dial between PSV and waste1 @00:52:05.45 PSR.select :air @00:52:05.47 PSV.dial PSR @00:52:06.01 CSV.dial :air @00:52:06.19 PS.reconfigure :fast @00:52:06.24 PS.seek 2.5ml @00:52:06.49 CS.reconfigure :fast @00:52:06.54 CS.seek 10ml @00:52:28.94 PSV.dial PTV @00:52:29.20 Pumping wcr puck from tube 2 up to 35psi... @00:52:29.22 CBV.dial SSV @00:52:29.66 delay 5 seconds @00:52:30.50 CBV.dial between CSV and waste2b @00:52:31.42 CSV.dial between air and CTV @00:52:31.74 CS.seek 7.416ml @00:52:34.68 PSV.dial PSR @00:52:35.39 delay 5 seconds @00:52:38.30 CTV.dial CSV,avoiding: :air @00:52:38.33 CSV.dial CTV @00:52:39.10 CS.reconfigure :pressurize @00:52:39.15 CS.seek 0ml @00:52:40.41 PSV.dial PTV @00:52:41.13 delay 5 seconds @00:52:46.14 PSV.dial :air @00:52:46.86 PS.seek 0ml @00:52:53.19 CBV.dial :waste2b @00:52:53.52 CS.reconfigure :default @00:52:53.57 CS.seek 0ml @00:52:59.48 delay 1:00 @00:53:09.47 Additional air clear @00:53:09.48 PSR.select :air @00:53:09.50 PSV.dial PSR @00:53:10.80 Pulling 2ml of PSR.air @00:53:10.81 PSR.select :air @00:53:10.83 PS.reconfigure :default @00:53:10.87 PS.seek 2ml @00:53:32.03 delay 5 seconds @00:53:37.05 PTV.dial :puck @00:53:37.07 PSV.dial PTV @00:53:37.78 PBV.dial :waste1 @00:53:38.20 PS.seek 1.5ml @00:53:44.03 delay 3 seconds @00:53:47.05 PBV.dial between PSV and waste1 @00:53:47.41 delay 1 second @00:53:48.42 PSV.dial :waste1 @00:53:49.43 PS.seek 0ml @00:53:59.50 CTV.dial :air,avoiding: :intake @00:54:00.75 Fix #1 of 2 (with RNAlater) for 10:00 @00:54:00.77 CSV.dial CSR @00:54:01.24 Pulling 2ml of CSR.RNAlater @00:54:01.25 CSR.select :RNAlater @00:54:01.36 CS.seek 2ml @00:54:05.55 Processing Puck Heater at 28.1C before imaging @00:54:05.56 PH.coast @00:54:05.68 Imaging da puck in Processing Clamp while storing it to tube 1 @00:54:05.83 SC.reconfigure :tight @00:54:05.88 SC.seek 1 @00:54:07.20 delay 5 seconds @00:54:09.55 SC.configure :limp @00:54:09.74 Elbow.reconfigure :tight @00:54:09.78 Elbow.seek 1 @00:54:11.54 Forearm.reconfigure :tight @00:54:11.58 Forearm.seek 1 + 700 counts @00:54:12.22 CSR.select :air @00:54:12.33 CS.reconfigure :fast @00:54:12.37 CS.seek 10ml @00:54:12.64 SE.reconfigure :pick @00:54:12.69 SE.seek :beamBreak @00:54:14.36 SE.configure :default @00:54:14.51 Elbow.reconfigure :limp @00:54:14.55 Forearm.reconfigure :loose @00:54:14.59 Forearm.seek 1 @00:54:14.99 Elbow.hold @00:54:15.05 Elbow.reconfigure :tight @00:54:15.14 SE.seek 21.34 Pucks below Top Plate @00:54:15.65 Forearm.force -20 @00:54:15.69 Forearm.seek 1 + 319 counts @00:54:15.96 Forearm.reconfigure :tight @00:54:16.00 Forearm.seek 1 + 700 counts @00:54:16.32 SE.seek 22.34 Pucks below Top Plate @00:54:16.89 Forearm.reconfigure :loose @00:54:16.93 Forearm.seek :retract @00:54:18.42 Elbow.seek PC @00:54:21.13 Elbow.reconfigure :loose @00:54:21.22 Forearm.seek PC @00:54:22.29 Hand.close @00:54:22.96 PC.seek :open @00:54:26.97 Forearm.seek :retract @00:54:28.08 Elbow.reconfigure :tight @00:54:28.13 Elbow.seek 1 @00:54:30.76 Forearm.reconfigure :tight @00:54:30.80 Forearm.seek 1 + 700 counts @00:54:31.05 Pumping wcr puck from tube 2 up to 35psi... @00:54:31.06 CBV.dial SSV @00:54:31.79 Hand.open @00:54:32.33 CBV.dial between CSV and waste2b @00:54:32.64 SE.seek 21.99 Pucks below Top Plate @00:54:32.91 SE.reconfigure :seal @00:54:32.95 SE.seek 20.88 Pucks below Top Plate @00:54:33.25 CSV.dial between air and CTV @00:54:33.50 Lights, camera, ACTION! @00:54:33.54 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22nov1000h1000ml.tif @00:54:33.90 CS.seek 7.283ml @00:54:40.77 CTV.dial CSV,avoiding: :air @00:54:40.81 CSV.dial CTV @00:54:42.10 CS.reconfigure :pressurize @00:54:42.15 CS.seek 0ml @00:54:51.04 SX MX5/16 (USB:11): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 13.815 seconds /var/log/esp/da22nov1000h1000ml.tif: TIFF Upload Complete @00:54:51.07 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22nov1000h1000ml15s.tif @00:54:53.31 Waiting >20 seconds for da to relinquish FlushPuck @00:54:59.01 delay 10:00 @00:55:06.81 SX MX5/16 (USB:11): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22nov1000h1000ml15s.tif: TIFF Upload Complete @00:55:06.83 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da22nov1000h1000ml25s.tif @00:55:32.56 SX MX5/16 (USB:11): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da22nov1000h1000ml25s.tif: TIFF Upload Complete @00:55:32.58 Gate.power :camera,:OFF @00:55:32.63 DAfans.email "13.815 sec auto-exposure",Subject: "da22nov1000h1000ml images!" @00:55:32.90 Elbow.seek 1 @00:55:33.08 Forearm.seek 1 + 700 counts @00:55:33.16 SE.reconfigure :gently @00:55:33.20 SE.seek 21.59 Pucks below Top Plate @00:55:33.89 Elbow.reconfigure :limp @00:55:33.94 Forearm.reconfigure :loose @00:55:34.00 Forearm.seek 1 @00:55:34.39 Elbow.hold @00:55:34.47 Elbow.reconfigure :tight @00:55:34.52 SE.reconfigure :default @00:55:34.57 SE.seek 21.78 Pucks below Top Plate @00:55:34.98 Forearm.force -20 @00:55:35.02 Forearm.seek 1 + 319 counts @00:55:35.41 Forearm.reconfigure :tight @00:55:35.47 Forearm.seek 1 + 700 counts @00:55:35.74 SE.seek :down @00:55:36.87 Forearm.reconfigure :loose @00:55:36.91 Forearm.seek :retract @00:55:36.98 SE.stop @00:55:37.91 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @00:55:38.11 Elbow.seek FlushPuck::Garage @00:55:40.18 Elbow.reconfigure :loose @00:55:40.39 Forearm.seek :garage @00:55:41.55 Hand.close @00:55:43.61 Forearm.seek :retract @00:55:44.71 Elbow.reconfigure :tight @00:55:44.75 Elbow.seek PC @00:55:46.05 Elbow.reconfigure :loose @00:55:46.20 Forearm.reconfigure :tight @00:55:46.28 Forearm.seek PC @00:55:47.98 PC.seek :closed @00:55:51.94 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.45 @00:55:52.10 Hand.open @00:55:52.99 Forearm.reconfigure :loose @00:55:53.09 Forearm.seek :retract @00:55:54.20 PSR.select :air @00:55:54.24 PSV.dial PSR @00:55:54.96 Pulling 0.5ml of PSR.flush @00:55:54.98 PSR.select :flush @00:55:55.22 PS.seek 0.5ml @00:56:01.28 delay 5 seconds @00:56:06.36 PSR.select :air @00:56:06.56 PS.seek 2.5ml @00:56:27.73 delay 1 second @00:56:28.77 PRV.dial :waste2,avoiding: :ab2 @00:56:30.63 PTV.dial :PRVmixing @00:56:31.90 PSV.dial :mixloop @00:56:32.95 PS.seek :empty @00:56:59.16 PSR.select :air @00:56:59.20 PSV.dial PSR @00:57:00.33 Pulling 0.5ml of PSR.flush @00:57:00.35 PSR.select :flush @00:57:00.52 PS.seek 0.5ml @00:57:06.50 delay 5 seconds @00:57:11.53 PSR.select :air @00:57:11.72 PS.seek 2.5ml @00:57:32.93 delay 1 second @00:57:33.96 PBV.dial :waste1 @00:57:34.34 PTV.dial :mixing @00:57:35.09 PSV.dial :mixloop @00:57:36.11 PS.seek :empty @00:58:02.42 PSV.dial PSR @00:58:03.42 PSR.select :flush @00:58:03.45 upsync @00:58:03.56 PS.seek 1ml @00:58:14.71 PSR.select :air @00:58:14.82 PS.seek 2.5ml @00:58:30.89 PSV.dial PTV @00:58:31.61 PTV.dial :puck @00:58:32.32 PBV.dial :waste1 @00:58:32.34 PS.seek 0ml @00:58:58.49 PSV.dial PSR @00:58:59.21 PSR.select :flush @00:58:59.32 PS.seek 0.5ml @00:59:05.30 PSR.select :air @00:59:05.41 PS.seek 2.5ml @00:59:26.55 PSV.dial PTV @00:59:27.27 PBV.dial PSV @00:59:27.81 PS.seek 0ml @00:59:54.02 PSV.dial PBV @00:59:54.48 PS.seek 2.5ml @01:00:20.73 PSV.dial :waste1 @01:00:21.44 PS.seek 0ml @01:00:47.70 PSV.dial PSR @01:00:48.14 PSR.select :air @01:00:48.17 PS.seek 2.5ml @01:01:14.33 PSV.dial PTV @01:01:15.03 PBV.dial PSV @01:01:15.05 PS.seek 0ml @01:01:41.26 PSV.dial PBV @01:01:41.72 PS.seek 2.5ml @01:02:07.98 PSV.dial :waste1 @01:02:08.69 PS.seek 0ml @01:02:34.92 PSV.dial PSR @01:02:35.48 PSR.select :flush @01:02:35.60 PS.seek 1ml @01:02:46.67 PSR.select :air @01:02:46.78 PS.seek 2.5ml @01:03:02.89 PSV.dial PTV @01:03:03.61 PTV.dial :puck @01:03:03.62 PBV.dial :waste1 @01:03:04.14 PS.seek 0ml @01:03:30.29 PSV.dial PSR @01:03:31.46 PSR.select :flush @01:03:31.58 PS.seek 0.5ml @01:03:37.45 PSR.select :air @01:03:37.56 PS.seek 2.5ml @01:03:58.67 PSV.dial PTV @01:03:59.39 PBV.dial PSV @01:03:59.92 PS.seek 0ml @01:04:26.04 PSV.dial PBV @01:04:26.50 PS.seek 2.5ml @01:04:52.66 PSV.dial :waste1 @01:04:53.37 PS.seek 0ml @01:04:59.03 CBV.dial :waste2b @01:04:59.36 CS.reconfigure :default @01:04:59.40 CS.seek 0ml @01:05:02.12 delay 1:00 @01:05:19.51 PSV.dial PSR @01:05:19.95 PSR.select :air @01:05:19.97 PS.seek 2.5ml @01:05:46.10 PSV.dial PTV @01:05:46.80 PBV.dial PSV @01:05:46.82 PS.seek 0ml @01:06:02.14 CTV.dial :air,avoiding: :intake @01:06:03.39 Fix #2 of 2 (with RNAlater) for 10:00 @01:06:03.40 CSV.dial CSR @01:06:03.87 Pulling 2ml of CSR.RNAlater @01:06:03.89 CSR.select :RNAlater @01:06:04.00 CS.seek 2ml @01:06:09.81 delay 5 seconds @01:06:13.04 PSV.dial PBV @01:06:13.61 PS.seek 2.5ml @01:06:14.82 CSR.select :air @01:06:14.94 CS.reconfigure :fast @01:06:14.98 CS.seek 10ml @01:06:33.18 Pumping wcr puck from tube 2 up to 35psi... @01:06:33.19 CBV.dial SSV @01:06:34.46 CBV.dial between CSV and waste2b @01:06:35.38 CSV.dial between air and CTV @01:06:35.97 CS.seek 7.269ml @01:06:39.82 PSV.dial :waste1 @01:06:40.54 PS.seek 0ml @01:06:42.75 CTV.dial CSV,avoiding: :air @01:06:42.77 CSV.dial CTV @01:06:44.11 CS.reconfigure :pressurize @01:06:44.15 CS.seek 0ml @01:07:01.14 delay 10:00 @01:07:06.73 Garaging FlushPuck in Processing Clamp @01:07:06.80 Elbow.reconfigure :tight @01:07:06.84 Elbow.seek PC @01:07:06.95 Elbow.reconfigure :loose @01:07:07.05 Forearm.seek PC @01:07:08.13 Hand.close @01:07:08.68 PC.seek :open @01:07:12.68 Forearm.seek :retract @01:07:13.73 Elbow.reconfigure :tight @01:07:13.77 Elbow.seek :garage @01:07:15.11 Elbow.reconfigure :loose @01:07:15.20 Forearm.reconfigure :tight @01:07:15.24 Forearm.seek :garage @01:07:16.36 Hand.open @01:07:17.11 Forearm.reconfigure :loose @01:07:17.15 Forearm.seek :retract @01:17:01.16 CBV.dial :waste2b @01:17:01.49 CS.reconfigure :default @01:17:01.54 CS.seek 0ml @01:17:04.07 delay 1:00 @01:18:04.09 CTV.dial :air,avoiding: :intake @01:18:05.40 Storing wcr puck in Collection Clamp to tube 1 @01:18:05.78 Elbow.reconfigure :tight @01:18:05.82 Elbow.seek 1 @01:18:07.83 Forearm.reconfigure :tight @01:18:08.33 Forearm.seek 1 + 700 counts @01:18:09.34 SE.reconfigure :pick @01:18:09.39 SE.seek :beamBreak @01:18:11.02 SE.configure :default @01:18:11.17 Elbow.reconfigure :limp @01:18:11.21 Forearm.reconfigure :loose @01:18:11.26 Forearm.seek 1 @01:18:11.65 Elbow.hold @01:18:11.72 Elbow.reconfigure :tight @01:18:11.81 SE.seek 22.35 Pucks below Top Plate @01:18:12.32 Forearm.force -20 @01:18:12.37 Forearm.seek 1 + 319 counts @01:18:12.68 Forearm.reconfigure :tight @01:18:12.73 Forearm.seek 1 + 700 counts @01:18:13.01 SE.seek 23.35 Pucks below Top Plate @01:18:13.57 Forearm.reconfigure :loose @01:18:13.62 Forearm.seek :retract @01:18:14.55 Elbow.seek CC @01:18:16.15 Elbow.reconfigure :loose @01:18:16.24 Forearm.seek CC @01:18:17.19 Hand.close @01:18:17.78 CC.seek :open @01:18:22.22 Forearm.seek :retract @01:18:23.19 Elbow.reconfigure :tight @01:18:23.23 Elbow.seek 1 @01:18:25.03 Elbow.reconfigure :limp @01:18:25.08 Forearm.seek 1 @01:18:26.20 Elbow.hold @01:18:26.27 Elbow.reconfigure :tight @01:18:26.31 Hand.open @01:18:27.15 Forearm.force -20 @01:18:27.19 Forearm.seek 1 + 320 counts @01:18:27.52 Forearm.reconfigure :tight @01:18:27.57 Forearm.seek 1 + 700 counts @01:18:27.86 SE.seek :down @01:18:28.99 Forearm.reconfigure :loose @01:18:29.05 Forearm.seek :retract @01:18:29.07 SE.stop @01:18:30.00
Starting finalPurge @01:18:30.02 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @01:18:30.19 Elbow.seek FlushPuck::Garage @01:18:31.99 Elbow.reconfigure :loose @01:18:32.09 Forearm.seek :garage @01:18:33.20 Hand.close @01:18:33.89 Forearm.seek :retract @01:18:34.91 Elbow.reconfigure :tight @01:18:34.95 Elbow.seek CC @01:18:37.86 Elbow.reconfigure :loose @01:18:37.95 Forearm.reconfigure :tight @01:18:37.99 Forearm.seek CC @01:18:39.31 CC.seek :closed @01:18:43.55 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.87 @01:18:43.62 Hand.open @01:18:44.46 Forearm.reconfigure :loose @01:18:44.50 Forearm.seek :retract @01:18:45.97 Flushing the Collection Valve::Manifold @01:18:46.08 CSV.dial CSR @01:18:46.70 Pulling 5ml of CSR.flush @01:18:46.71 CSR.select :flush @01:18:46.82 CS.seek 5ml @01:19:00.19 delay 5 seconds @01:19:05.21 CSR.select :air @01:19:05.35 CS.reconfigure :fast @01:19:05.40 CS.seek 7.499ml @01:19:11.75 CSV.dial :waste @01:19:12.99 CS.reconfigure :default @01:19:13.03 CS.seek 0ml @01:19:32.78 Flushing lower manifold's waste1 port @01:19:32.90 CSV.dial :flush @01:19:33.35 CS.reconfigure :fast @01:19:33.39 CS.seek 5ml @01:19:45.16 delay 5 seconds @01:19:50.17 CSV.dial :air @01:19:50.67 CS.seek 6.999ml @01:19:55.94 CSV.dial CTV @01:19:55.97
CTV.dial CSV @01:19:57.26 CBV.dial :waste1 @01:19:57.99 CS.seek 0ml @01:20:13.87 Flushing lower manifold's waste2 port @01:20:13.99 CSV.dial :flush @01:20:14.71 CS.seek 5ml @01:20:26.28 delay 5 seconds @01:20:31.30 CSV.dial :air @01:20:31.92 CS.seek 7ml @01:20:37.16 CSV.dial CTV @01:20:37.18
CTV.dial CSV @01:20:37.64 CBV.dial :waste2 @01:20:38.64 CS.seek 0ml @01:20:54.56 Flushing lower manifold's waste2b port @01:20:54.68 CSV.dial :flush @01:20:55.39 CS.seek 5ml @01:21:06.97 delay 5 seconds @01:21:11.98 CSV.dial :air @01:21:12.48 CS.seek 7ml @01:21:17.63 CSV.dial CTV @01:21:17.67
CTV.dial CSV @01:21:18.08 CBV.dial :waste2b @01:21:19.07 CS.seek 0ml @01:21:34.90 Disinfecting external Intake with kill @01:21:35.02 CSV.dial CSR @01:21:35.61 Pulling 7.999ml of CSR.kill @01:21:35.62 CSR.select :kill @01:21:35.74 CS.seek 8ml @01:21:53.91 delay 5 seconds @01:21:58.92 CSR.select :air @01:21:59.03 CS.seek 10ml @01:22:04.32 CSV.dial CBV @01:22:04.34
CBV.dial CSV @01:22:05.39 CTV.dial :intake @01:22:06.09 Intake.select :open @01:22:06.20 CS.seek 2ml @01:22:24.24 Intake.select :close @01:22:24.34 CTV.dial :air @01:22:25.08 CS.reconfigure :default @01:22:25.12 CS.seek 0ml @01:22:31.06 CSV.dial CSR @01:22:32.08 Pulling 5ml of CSR.flush @01:22:32.09 CSR.select :flush @01:22:32.20 CS.seek 5ml @01:22:45.65 delay 5 seconds @01:22:50.67 CSR.select :air @01:22:50.78 CS.seek 10ml @01:23:04.18 CSV.dial CBV @01:23:05.16 CS.seek 0ml @01:23:31.24 Garaging FlushPuck in Collection Clamp @01:23:31.31 Elbow.reconfigure :tight @01:23:31.35 Elbow.seek CC @01:23:31.46 Elbow.reconfigure :loose @01:23:31.55 Forearm.seek CC @01:23:32.55 Hand.close @01:23:33.15 CC.seek :open @01:23:37.48 Forearm.seek :retract @01:23:38.44 Elbow.reconfigure :tight @01:23:38.48 Elbow.seek :garage @01:23:41.59 Elbow.reconfigure :loose @01:23:41.68 Forearm.reconfigure :tight @01:23:41.72 Forearm.seek :garage @01:23:42.83 Hand.open @01:23:43.58 Forearm.reconfigure :loose @01:23:43.62 Forearm.seek :retract @01:23:44.65 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 22:34:19 @01:23:44.69 Asleep after completing DA_WCR_11922 assays @01:23:44.71 GoodNews.email "{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@01:23:33, 23.4C, 27% humidity, 15.7psia, 14.031V, 0.337A, 0.337A avg, 4.72W\nSampled 700.0ml",Subject: "Asleep after completing DA_WCR_11922 assays" @01:24:19.11
Uploading Logs @01:25:38.83 upsync @21:14:12.43UTC10-Nov-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @21:14:15.05 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @21:14:18.95 Polling Can every 10:00 @21:14:18.98 Cleanly exited at 21:08:59UTC10-Nov-22 @21:14:19.02 Loading /home/esp/esp2/mission/DA_WCR_111022.rb mission @21:14:19.07 Preparing to sleep until + 00:00:05 @21:14:19.08 GoodNews.email "Starting DA_WCR_111022 w/pucks in tube 2\n{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:14:18, 23.7C, 27% humidity, 15.7psia, 14.050V, 0.257A, 0.257A avg, 3.61W",Subject: "DA_WCR_111022 begun" @21:14:19.13
Asleep til + 00:00:05 @21:14:19.16 Continuing at 21:14 @21:14:19.41 Gate.power :core,:OFF @21:14:19.42 Gate.power :raw,:OFF @21:14:19.43 Shutdown @21:14:23.42 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_111022.rb:14\n{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:14:19, 23.7C, 27% humidity, 15.7psia, 14.050V, 0.257A, 0.257A avg, 3.61W",Subject: "Asleep until 21:14" @21:14:24.05
Powering core bus @21:14:29.70 Gate.power :raw,:ON @21:14:30.99 CS.configure :default @21:14:31.26 PS.configure :default @21:14:31.44 SS.configure :default @21:14:31.64 CC.configure :default @21:14:31.82 PC.configure :default @21:14:32.04 Forearm.configure :loose @21:14:32.20 Elbow.configure :loose @21:14:32.40 SC.configure :loose @21:14:32.67 SE.configure :default @21:14:32.73 CH.coast @21:14:32.82 CH.configure :default @21:14:32.88 PH.coast @21:14:32.99 PH.configure :default @21:14:33.06 All dwarf controllers running firmware version 3.85 @21:14:33.24 Valve.reset @21:14:33.57 Intake.select :close @21:14:33.73 Exhaust.select :close @21:14:33.75 CTV.dial :air @21:14:34.01 PSV.dial :air @21:14:34.19 CSV.dial :waste1 @21:14:34.27
Forearm.reconfigure :loose @21:14:34.39 Forearm.home.seek :retract @21:14:34.77 SSV.dial :spit,avoiding: :exhaust @21:14:35.18 PS.reconfigure :default @21:14:35.25 PS.home.seek 0ml @21:14:35.38 Hand.close @21:14:35.45
Elbow.jog 1500 @21:14:35.66 CS.reconfigure :fast @21:14:35.72 SS.reconfigure :default @21:14:35.90 CS.home.seek 0ml @21:14:35.96 SS.home.seek 0ml @21:14:36.20
Elbow.reconfigure :loose @21:14:36.28 Elbow.home.seek :clear @21:14:40.83 SE.reconfigure :default @21:14:40.91 SE.home.seek :down @21:14:41.55 SC.reconfigure :tight @21:14:41.63 SC.home.seek :initial @21:14:43.83 PS.stop @21:15:11.75 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @21:15:11.78
Retry #1 of 3 @21:15:11.90 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @21:15:16.17 SC.configure :limp @21:15:16.28 Forearm.seek :retract @21:15:16.40 Elbow.seek :hammer @21:15:17.63 Forearm.reconfigure :tight @21:15:17.71 Forearm.seek retracted @21:15:18.40 PC.reconfigure :default @21:15:18.46
CC.reconfigure :default @21:15:18.64 PC.home.seek :open @21:15:18.72
CC.home.seek :open @21:15:22.45 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @21:15:22.49 Notice.email "At 21:15:22 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_111022.rb:14\n{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:15:22, 23.7C, 27% humidity, 15.7psia, 14.036V, 0.239A, 0.245A avg, 3.35W",Subject: "Started DA_WCR_111022 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @21:15:22.53
Starting initialPurge @21:15:22.59 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:15:22.74 Hand.open @21:15:23.78 Elbow.reconfigure :tight @21:15:23.87 Elbow.seek FlushPuck::Garage @21:15:25.92 Elbow.reconfigure :loose @21:15:26.14 Forearm.reconfigure :loose @21:15:26.22 Forearm.seek :garage @21:15:27.38 Hand.close @21:15:28.24 Forearm.seek :retract @21:15:29.35 Elbow.reconfigure :tight @21:15:29.43 Elbow.seek CC @21:15:32.49 Elbow.reconfigure :loose @21:15:32.71 Forearm.reconfigure :tight @21:15:32.77 Forearm.seek CC @21:15:34.45 CC.seek :closed @21:15:38.77 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.86 @21:15:38.91 Hand.open @21:15:39.77 Forearm.reconfigure :loose @21:15:39.83 Forearm.seek :retract @21:15:40.98 Flushing the Collection Valve::Manifold @21:15:41.26 CSV.dial CSR @21:15:42.75 Pulling 5ml of CSR.flush @21:15:42.77 CSR.select :flush @21:15:42.93 CS.reconfigure :default @21:15:43.01 CS.seek 5ml @21:15:56.56 delay 5 seconds @21:16:01.57 CSR.select :air @21:16:01.80 CS.reconfigure :fast @21:16:01.88 CS.seek 7.501ml @21:16:08.29 CSV.dial :waste @21:16:09.56 CS.reconfigure :default @21:16:09.65 CS.seek 0ml @21:16:22.28 upsync @21:16:29.38
Purging external Intake @21:16:29.49 CSV.dial :flush @21:16:29.95 CS.reconfigure :fast @21:16:29.99 CS.seek 10ml @21:16:52.54 delay 5 seconds @21:16:57.56 CSV.dial CBV @21:16:57.59
CBV.dial CSV @21:16:58.32 CTV.dial :intake @21:16:59.00 Intake.select :open @21:16:59.11 CS.seek 0ml @21:17:21.63 Intake.select :close @21:17:21.73 CTV.dial :air @21:17:22.44 Flushing upper manifold @21:17:22.56 CSV.dial :flush @21:17:23.28 CS.seek 5ml @21:17:35.00 delay 5 seconds @21:17:40.02 CSV.dial :air @21:17:40.52 CS.seek 7.001ml @21:17:45.70 CSV.dial CBV @21:17:45.72
CBV.dial CSV @21:17:46.83 CTV.dial :air @21:17:46.85 CS.seek 0ml @21:18:02.82 delay 7 seconds @21:18:09.84 CS.seek 3ml @21:18:17.16 CSV.dial :waste @21:18:17.59 CS.seek 0ml @21:18:24.98 CSV.dial :flush @21:18:25.40 CS.seek 3ml @21:18:32.69 delay 5 seconds @21:18:37.71 CSV.dial :air @21:18:38.20 CS.seek 5ml @21:18:43.47 CSV.dial :air @21:18:43.49 CS.seek 0ml @21:18:55.09 delay 7 seconds @21:19:02.12 CS.seek 3ml @21:19:09.47 CSV.dial :waste @21:19:10.18 CS.seek 0ml @21:19:17.54 Garaging FlushPuck in Collection Clamp @21:19:17.60 Elbow.reconfigure :tight @21:19:17.64 Elbow.seek CC @21:19:17.76 Elbow.reconfigure :loose @21:19:17.84 Forearm.seek CC @21:19:18.81 Hand.close @21:19:19.37 CC.seek :open @21:19:23.70 Forearm.seek :retract @21:19:24.70 Elbow.reconfigure :tight @21:19:24.74 Elbow.seek :garage @21:19:27.63 Elbow.reconfigure :loose @21:19:27.71 Forearm.reconfigure :tight @21:19:27.76 Forearm.seek :garage @21:19:28.85 Hand.open @21:19:29.68 Forearm.reconfigure :loose @21:19:30.13 Forearm.seek :retract @21:19:31.20 da sampling at most 1000ml, wcr at most 1000ml @21:19:31.23 DAfans.email "Started da in tube 2 at 21:19:31UTC10-Nov-22\n{1 => 24,\n 2 => 9,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:15:22, 23.7C, 27% humidity, 15.7psia, 14.036V, 0.239A, 0.245A avg, 3.35W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @21:19:31.26
Loading sh1 puck from tube 2 into Collection Clamp @21:19:31.49 SC.reconfigure :tight @21:19:31.53 SC.seek 2 @21:19:44.53 SC.configure :limp @21:19:45.04 Elbow.reconfigure :tight @21:19:45.12 Elbow.seek 2 @21:19:47.30 Forearm.reconfigure :tight @21:19:47.38 Forearm.seek 2 + 700 counts @21:19:48.57 SE.reconfigure :pick @21:19:48.69 SE.seek :beamBreak @21:19:51.30 SE.configure :default @21:19:51.60 Elbow.seek 2 @21:19:52.02 Forearm.seek 2 + 300 counts @21:19:52.34 Forearm.reconfigure :loose @21:19:52.43 Hand.close @21:19:53.07 SE.seek :down @21:19:53.45
Forearm.seek :retract @21:19:54.59 Elbow.seek CC @21:19:55.51 SE.stop @21:19:56.33
Elbow.reconfigure :loose @21:19:56.55 Forearm.reconfigure :tight @21:19:56.63 Forearm.seek CC @21:19:58.14 CC.seek :closed @21:20:02.53 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.73 @21:20:02.55 Hand.open @21:20:03.38 Forearm.reconfigure :loose @21:20:03.46 Forearm.seek :retract @21:20:04.53 collectSample 1000ml w/bubble point of 24psi @21:20:04.62 Priming 7ml @21:20:04.64 Intake.select :close @21:20:04.66 Exhaust.select :close @21:20:04.68 SS.hold @21:20:05.07 Calibrating pressure and temperature sensors @21:20:05.16 CTV.dial :air @21:20:05.20 SSV.dial :spit,avoiding: :exhaust @21:20:05.24 SS.reconfigure :fast @21:20:05.32 SS.seek 5ml @21:20:08.47 SSV.dial CBV @21:20:08.55
CBV.dial SSV @21:20:09.83 delay 3.8 seconds @21:20:13.85 SSV.dial :spit @21:20:15.17 SS.seek 0ml @21:20:17.70 SSV.dial CBV @21:20:19.00 CTV.dial :intake @21:20:19.77 SSV.dial CBV @21:20:19.83
CBV.dial SSV @21:20:19.85 Allowing 40s for 7.0ml of remaining 7.0ml @21:20:19.87 Intake.select :open @21:20:22.11 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:20:22.15 SS.reconfigure :inhaling 2.14ml/s @21:20:22.23 SS.seek 7ml @21:20:28.87 Stroke completed -- waiting for pressure to equalize ... @21:20:28.89 SS.reconfigure :exhale @21:20:28.97 SS.hold @21:20:33.55 Intake.select :close @21:20:33.76 SSV.dial :exhaust @21:20:34.53 Exhaust.select :open @21:20:34.69 SS.seek 0ml @21:20:38.84 Exhaust.select :close @21:20:39.00 Sampling 1000.0ml @21:20:39.04 Intake.select :close @21:20:39.06 Exhaust.select :close @21:20:39.08 SS.reconfigure :default @21:20:39.16 SS.hold @21:20:39.55 CTV.dial :intake @21:20:39.59 SSV.dial CBV @21:20:39.63
CBV.dial SSV @21:20:40.44 Allowing 130s for 25.0ml of remaining 1000.0ml @21:20:40.46 Intake.select :open @21:20:42.69 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:20:42.74 SS.reconfigure :inhaling 2.14ml/s @21:20:42.82 SS.seek 25ml @21:20:47.57 Vacuum too high -- waiting up to 40 seconds @21:20:47.59 SS.reconfigure :exhale @21:20:47.65 SS.hold @21:20:48.47 Intake @11.9psia, vacuum from 6.4 to 9.4psi @21:20:48.49 SS.reconfigure :inhaling 1.93ml/s @21:20:49.62 SS.seek 25ml @21:20:52.60 Vacuum too high -- waiting up to 40 seconds @21:20:52.62 SS.reconfigure :exhale @21:20:52.79 SS.hold @21:20:54.14 Intake @16.7psia, vacuum from 10.0 to 13.0psi @21:20:54.18 SS.reconfigure :inhaling 1.74ml/s @21:20:54.27 SS.seek 25ml @21:20:56.74 Vacuum too high -- waiting up to 40 seconds @21:20:56.76 SS.reconfigure :exhale @21:20:56.86 SS.hold @21:20:57.57 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:20:57.61 SS.reconfigure :inhaling 1.57ml/s @21:20:57.69 SS.seek 25ml @21:21:00.87 Vacuum too high -- waiting up to 40 seconds @21:21:00.89 SS.reconfigure :exhale @21:21:01.03 SS.hold @21:21:01.61 Intake @13.1psia, vacuum from 7.6 to 10.6psi @21:21:01.64 SS.reconfigure :inhaling 1.41ml/s @21:21:01.74 SS.seek 25ml @21:21:04.33 Vacuum too high -- waiting up to 40 seconds @21:21:04.35 SS.reconfigure :exhale @21:21:04.47 SS.hold @21:21:05.38 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:21:05.40 SS.reconfigure :inhaling 1.27ml/s @21:21:05.58 SS.seek 25ml @21:21:08.38 Vacuum too high -- waiting up to 40 seconds @21:21:08.40 SS.reconfigure :exhale @21:21:08.48 SS.hold @21:21:09.11 Intake @14.1psia, vacuum from 8.6 to 11.6psi @21:21:09.14 SS.reconfigure :inhaling 1.14ml/s @21:21:09.21 SS.seek 25ml @21:21:14.42 Vacuum too high -- waiting up to 40 seconds @21:21:14.48 SS.reconfigure :exhale @21:21:14.56 SS.hold @21:21:15.08 Intake @13.3psia, vacuum from 7.8 to 10.8psi @21:21:15.12 SS.reconfigure :inhaling 1.03ml/s @21:21:15.20 SS.seek 25ml @21:21:20.03 Stroke completed -- waiting for pressure to equalize ... @21:21:20.04 SS.reconfigure :exhale @21:21:20.11 SS.hold @21:21:21.69 Intake.select :close @21:21:21.90 SSV.dial :exhaust @21:21:22.67 Exhaust.select :open @21:21:22.84 SS.seek 0ml @21:21:31.69 Exhaust.select :close @21:21:31.84 SSV.dial CBV @21:21:31.90
CBV.dial SSV @21:21:32.70 Allowing 130s for 25.0ml of remaining 975.0ml @21:21:32.72 Intake.select :open @21:21:35.00 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:21:35.04 SS.reconfigure :inhaling 1.03ml/s @21:21:35.12 SS.seek 25ml @21:21:37.72 upsync @21:21:44.83
Vacuum too high -- waiting up to 40 seconds @21:21:44.84 SS.reconfigure :exhale @21:21:44.88 SS.hold @21:21:45.26 Intake @12.6psia, vacuum from 7.1 to 10.1psi @21:21:45.27 SS.reconfigure :inhaling 0.93ml/s @21:21:45.32 SS.seek 25ml @21:21:53.11 Vacuum too high -- waiting up to 40 seconds @21:21:53.12 SS.reconfigure :exhale @21:21:53.16 SS.hold @21:21:54.11 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:21:54.13 SS.reconfigure :inhaling 0.84ml/s @21:21:54.17 SS.seek 25ml @21:22:07.99 Stroke completed -- waiting for pressure to equalize ... @21:22:08.00 SS.reconfigure :exhale @21:22:08.04 SS.hold @21:22:09.58 Intake.select :close @21:22:09.72 SSV.dial :exhaust @21:22:10.44 Exhaust.select :open @21:22:10.55 SS.seek 0ml @21:22:19.36 Exhaust.select :close @21:22:19.47 SSV.dial CBV @21:22:19.49
CBV.dial SSV @21:22:20.23 Allowing 130s for 25.0ml of remaining 950.0ml @21:22:20.24 Intake.select :open @21:22:22.38 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:22:22.40 SS.reconfigure :inhaling 0.84ml/s @21:22:22.44 SS.seek 25ml @21:22:54.64 Stroke completed -- waiting for pressure to equalize ... @21:22:54.65 SS.reconfigure :exhale @21:22:54.69 SS.hold @21:22:56.63 Intake.select :close @21:22:56.77 SSV.dial :exhaust @21:22:57.49 Exhaust.select :open @21:22:57.59 SS.seek 0ml @21:23:06.88 Exhaust.select :close @21:23:06.98 SSV.dial CBV @21:23:07.03
CBV.dial SSV @21:23:07.72 Allowing 130s for 25.0ml of remaining 925.0ml @21:23:07.73 Intake.select :open @21:23:09.88 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:23:09.90 SS.reconfigure :inhaling 0.84ml/s @21:23:09.94 SS.seek 25ml @21:23:34.17 Vacuum too high -- waiting up to 40 seconds @21:23:34.18 SS.reconfigure :exhale @21:23:34.22 SS.hold @21:23:34.60 Intake @12.8psia, vacuum from 7.3 to 10.3psi @21:23:34.61 SS.reconfigure :inhaling 0.76ml/s @21:23:34.65 SS.seek 25ml @21:23:42.92 Stroke completed -- waiting for pressure to equalize ... @21:23:42.93 SS.reconfigure :exhale @21:23:42.97 SS.hold @21:23:44.51 Intake.select :close @21:23:44.65 SSV.dial :exhaust @21:23:45.38 Exhaust.select :open @21:23:45.48 SS.seek 0ml @21:23:54.30 Exhaust.select :close @21:23:54.40 SSV.dial CBV @21:23:54.43
CBV.dial SSV @21:23:55.16 Allowing 130s for 25.0ml of remaining 900.0ml @21:23:55.17 Intake.select :open @21:23:57.32 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:23:57.34 SS.reconfigure :inhaling 0.76ml/s @21:23:57.38 SS.seek 25ml @21:24:32.86 Stroke completed -- waiting for pressure to equalize ... @21:24:32.87 SS.reconfigure :exhale @21:24:32.91 SS.hold @21:24:34.71 Intake.select :close @21:24:34.85 SSV.dial :exhaust @21:24:35.56 Exhaust.select :open @21:24:35.67 SS.seek 0ml @21:24:44.87 Exhaust.select :close @21:24:44.98 SSV.dial CBV @21:24:45.01
CBV.dial SSV @21:24:45.74 Allowing 130s for 25.0ml of remaining 875.0ml @21:24:45.75 Intake.select :open @21:24:47.90 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:24:47.92 SS.reconfigure :inhaling 0.76ml/s @21:24:47.96 SS.seek 25ml @21:25:22.51 Stroke completed -- waiting for pressure to equalize ... @21:25:22.52 SS.reconfigure :exhale @21:25:22.56 SS.hold @21:25:24.51 Intake.select :close @21:25:24.65 SSV.dial :exhaust @21:25:25.37 Exhaust.select :open @21:25:25.48 SS.seek 0ml @21:25:34.62 Exhaust.select :close @21:25:34.72 SSV.dial CBV @21:25:34.75
CBV.dial SSV @21:25:35.48 Allowing 130s for 25.0ml of remaining 850.0ml @21:25:35.49 Intake.select :open @21:25:37.64 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:25:37.66 SS.reconfigure :inhaling 0.76ml/s @21:25:37.70 SS.seek 25ml @21:26:12.37 Stroke completed -- waiting for pressure to equalize ... @21:26:12.38 SS.reconfigure :exhale @21:26:12.42 SS.hold @21:26:14.81 Intake.select :close @21:26:14.94 SSV.dial :exhaust @21:26:15.65 Exhaust.select :open @21:26:15.76 SS.seek 0ml @21:26:24.57 Exhaust.select :close @21:26:24.68 SSV.dial CBV @21:26:24.70
CBV.dial SSV @21:26:25.44 Allowing 130s for 25.0ml of remaining 825.0ml Intake.select :open @21:26:27.59 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:26:27.61 SS.reconfigure :inhaling 0.76ml/s @21:26:27.65 SS.seek 25ml @21:27:03.07 Stroke completed -- waiting for pressure to equalize ... @21:27:03.08 SS.reconfigure :exhale @21:27:03.12 SS.hold @21:27:06.02 Intake.select :close @21:27:06.16 SSV.dial :exhaust @21:27:06.89 Exhaust.select :open @21:27:07.00 SS.seek 0ml @21:27:16.13 Exhaust.select :close @21:27:16.24 SSV.dial CBV @21:27:16.26
CBV.dial SSV @21:27:17.00 Allowing 130s for 25.0ml of remaining 800.0ml @21:27:17.01 Intake.select :open @21:27:19.16 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:27:19.17 SS.reconfigure :inhaling 0.76ml/s @21:27:19.21 SS.seek 25ml @21:27:53.80 Stroke completed -- waiting for pressure to equalize ... @21:27:53.81 SS.reconfigure :exhale @21:27:53.86 SS.hold @21:27:56.82 Intake.select :close @21:27:56.96 SSV.dial :exhaust @21:27:57.66 Exhaust.select :open @21:27:57.77 SS.seek 0ml @21:28:06.76 Exhaust.select :close @21:28:06.86 SSV.dial CBV @21:28:06.91
CBV.dial SSV @21:28:07.60 Allowing 130s for 25.0ml of remaining 775.0ml @21:28:07.61 Intake.select :open @21:28:09.76 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:28:09.78 SS.reconfigure :inhaling 0.76ml/s @21:28:09.82 SS.seek 25ml @21:28:39.06 Vacuum too high -- waiting up to 40 seconds @21:28:39.07 SS.reconfigure :exhale @21:28:39.11 SS.hold @21:28:40.74 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:28:40.76 SS.reconfigure :inhaling 0.69ml/s @21:28:40.80 SS.seek 25ml @21:28:48.00 Stroke completed -- waiting for pressure to equalize ... @21:28:48.01 SS.reconfigure :exhale @21:28:48.05 SS.hold @21:28:50.44 Intake.select :close @21:28:50.58 SSV.dial :exhaust @21:28:51.30 Exhaust.select :open @21:28:51.41 SS.seek 0ml @21:29:00.66 Exhaust.select :close @21:29:00.77 SSV.dial CBV @21:29:00.79
CBV.dial SSV @21:29:01.54 Allowing 130s for 25.0ml of remaining 750.0ml @21:29:01.55 Intake.select :open @21:29:03.70 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:29:03.71 SS.reconfigure :inhaling 0.69ml/s @21:29:03.76 SS.seek 25ml @21:29:41.71 Stroke completed -- waiting for pressure to equalize ... @21:29:41.72 SS.reconfigure :exhale @21:29:41.76 SS.hold @21:29:45.11 Intake.select :close @21:29:45.25 SSV.dial :exhaust @21:29:45.97 Exhaust.select :open @21:29:46.08 SS.seek 0ml @21:29:55.02 Exhaust.select :close @21:29:55.12 SSV.dial CBV @21:29:55.15
CBV.dial SSV @21:29:55.88 Allowing 130s for 25.0ml of remaining 725.0ml @21:29:55.89 Intake.select :open @21:29:58.04 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:29:58.06 SS.reconfigure :inhaling 0.69ml/s @21:29:58.10 SS.seek 25ml @21:30:37.11 Stroke completed -- waiting for pressure to equalize ... @21:30:37.12 SS.reconfigure :exhale @21:30:37.16 SS.hold @21:30:40.60 Intake.select :close @21:30:40.74 SSV.dial :exhaust @21:30:41.44 Exhaust.select :open @21:30:41.55 SS.seek 0ml @21:30:50.52 Exhaust.select :close @21:30:50.62 SSV.dial CBV @21:30:50.66
CBV.dial SSV @21:30:51.36 Allowing 130s for 25.0ml of remaining 700.0ml @21:30:51.37 Intake.select :open @21:30:53.52 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:30:53.54 SS.reconfigure :inhaling 0.69ml/s @21:30:53.58 SS.seek 25ml @21:31:29.90 Vacuum too high -- waiting up to 40 seconds @21:31:29.91 SS.reconfigure :exhale @21:31:29.95 SS.hold @21:31:32.43 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:31:32.45 SS.reconfigure :inhaling 0.61ml/s @21:31:32.49 SS.seek 25ml @21:31:36.20 Stroke completed -- waiting for pressure to equalize ... @21:31:36.21 SS.reconfigure :exhale @21:31:36.25 SS.hold @21:31:38.19 Intake.select :close @21:31:38.33 SSV.dial :exhaust @21:31:39.05 Exhaust.select :open @21:31:39.16 SS.seek 0ml @21:31:48.41 Exhaust.select :close @21:31:48.51 SSV.dial CBV @21:31:48.54
CBV.dial SSV @21:31:49.27 Allowing 130s for 25.0ml of remaining 675.0ml @21:31:49.28 Intake.select :open @21:31:51.43 Intake @14.4psia, vacuum from 8.9 to 11.9psi @21:31:51.44 SS.reconfigure :inhaling 0.61ml/s @21:31:51.49 SS.seek 25ml @21:32:33.84 Stroke completed -- waiting for pressure to equalize ... @21:32:33.85 SS.reconfigure :exhale @21:32:33.89 SS.hold @21:32:37.33 Intake.select :close @21:32:37.46 SSV.dial :exhaust @21:32:38.17 Exhaust.select :open @21:32:38.28 SS.seek 0ml @21:32:47.61 Exhaust.select :close @21:32:47.71 SSV.dial CBV @21:32:47.76
CBV.dial SSV @21:32:48.45 Allowing 130s for 25.0ml of remaining 650.0ml @21:32:48.46 Intake.select :open @21:32:50.61 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:32:50.63 SS.reconfigure :inhaling 0.61ml/s @21:32:50.67 SS.seek 25ml @21:33:32.84 Stroke completed -- waiting for pressure to equalize ... @21:33:32.85 SS.reconfigure :exhale @21:33:32.89 SS.hold @21:33:37.05 Intake.select :close @21:33:37.19 SSV.dial :exhaust @21:33:37.94 Exhaust.select :open @21:33:38.07 SS.seek 0ml @21:33:46.89 Exhaust.select :close @21:33:46.99 SSV.dial CBV @21:33:47.02
CBV.dial SSV @21:33:47.76 Allowing 130s for 25.0ml of remaining 625.0ml @21:33:47.77 Intake.select :open @21:33:49.92 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:33:49.94 SS.reconfigure :inhaling 0.61ml/s @21:33:49.98 SS.seek 25ml @21:34:33.26 Stroke completed -- waiting for pressure to equalize ... @21:34:33.27 SS.reconfigure :exhale @21:34:33.31 SS.hold @21:34:38.83 Intake.select :close @21:34:39.43 SSV.dial :exhaust @21:34:40.15 Exhaust.select :open @21:34:40.26 SS.seek 0ml @21:34:49.17 Exhaust.select :close @21:34:49.27 SSV.dial CBV @21:34:49.31
CBV.dial SSV @21:34:50.03 Allowing 130s for 25.0ml of remaining 600.0ml @21:34:50.04 Intake.select :open @21:34:52.19 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:34:52.21 SS.reconfigure :inhaling 0.61ml/s @21:34:52.25 SS.seek 25ml @21:35:28.33 Vacuum too high -- waiting up to 40 seconds @21:35:28.34 SS.reconfigure :exhale @21:35:28.38 SS.hold @21:35:30.86 Intake @13.5psia, vacuum from 8.0 to 11.0psi @21:35:30.88 SS.reconfigure :inhaling 0.56ml/s @21:35:30.92 SS.seek 25ml @21:35:39.71 Stroke completed -- waiting for pressure to equalize ... @21:35:39.72 SS.reconfigure :exhale @21:35:39.76 SS.hold @21:35:43.82 Intake.select :close @21:35:43.95 SSV.dial :exhaust @21:35:44.66 Exhaust.select :open @21:35:44.77 SS.seek 0ml @21:35:53.74 Exhaust.select :close @21:35:53.84 SSV.dial CBV @21:35:53.87
CBV.dial SSV @21:35:54.60 Allowing 130s for 25.0ml of remaining 575.0ml @21:35:54.61 Intake.select :open @21:35:56.76 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:35:56.78 SS.reconfigure :inhaling 0.56ml/s @21:35:56.82 SS.seek 25ml @21:36:44.52 Stroke completed -- waiting for pressure to equalize ... @21:36:44.53 SS.reconfigure :exhale @21:36:44.57 SS.hold @21:36:49.32 Intake.select :close @21:36:49.46 SSV.dial :exhaust @21:36:50.18 Exhaust.select :open @21:36:50.29 SS.seek 0ml @21:36:59.29 Exhaust.select :close @21:36:59.39 SSV.dial CBV @21:36:59.41
CBV.dial SSV @21:37:00.16 Allowing 130s for 25.0ml of remaining 550.0ml @21:37:00.18 Intake.select :open @21:37:02.33 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:37:02.35 SS.reconfigure :inhaling 0.56ml/s @21:37:02.39 SS.seek 25ml @21:37:44.26 Vacuum too high -- waiting up to 40 seconds @21:37:44.27 SS.reconfigure :exhale @21:37:44.31 SS.hold @21:37:47.35 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:37:47.36 SS.reconfigure :inhaling 0.50ml/s @21:37:47.41 SS.seek 25ml @21:37:54.71 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @21:37:54.75 SS.hold @21:37:58.20 Intake.select :close @21:37:58.34 SSV.dial :exhaust @21:37:59.07 Exhaust.select :open @21:37:59.17 SS.seek 0ml @21:38:08.17 Exhaust.select :close @21:38:08.28 SSV.dial CBV @21:38:08.31
CBV.dial SSV @21:38:09.04 Allowing 130s for 25.0ml of remaining 525.0ml @21:38:09.05 Intake.select :open @21:38:11.20 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:38:11.21 SS.reconfigure :inhaling 0.50ml/s @21:38:11.26 SS.seek 25ml @21:39:04.44 Stroke completed -- waiting for pressure to equalize ... @21:39:04.45 SS.reconfigure :exhale @21:39:04.49 SS.hold @21:39:09.70 Intake.select :close @21:39:09.84 SSV.dial :exhaust @21:39:10.55 Exhaust.select :open @21:39:10.66 SS.seek 0ml @21:39:19.92 Exhaust.select :close @21:39:20.03 SSV.dial CBV @21:39:20.05
CBV.dial SSV @21:39:20.78 Allowing 130s for 25.0ml of remaining 500.0ml @21:39:20.80 Intake.select :open @21:39:22.95 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:39:22.96 SS.reconfigure :inhaling 0.50ml/s @21:39:23.00 SS.seek 25ml @21:40:15.97 Stroke completed -- waiting for pressure to equalize ... @21:40:15.98 SS.reconfigure :exhale @21:40:16.02 SS.hold @21:40:22.50 Intake.select :close @21:40:22.63 SSV.dial :exhaust @21:40:23.36 Exhaust.select :open @21:40:23.47 SS.seek 0ml @21:40:32.42 Exhaust.select :close @21:40:32.52 SSV.dial CBV @21:40:32.55
CBV.dial SSV @21:40:33.28 Allowing 130s for 25.0ml of remaining 475.0ml @21:40:33.29 Intake.select :open @21:40:35.44 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:40:35.46 SS.reconfigure :inhaling 0.50ml/s @21:40:35.50 SS.seek 25ml @21:41:26.16 Vacuum too high -- waiting up to 40 seconds @21:41:26.17 SS.reconfigure :exhale @21:41:26.21 SS.hold @21:41:30.38 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:41:30.40 SS.reconfigure :inhaling 0.46ml/s @21:41:30.44 SS.seek 25ml @21:41:34.79 Stroke completed -- waiting for pressure to equalize ... @21:41:34.80 SS.reconfigure :exhale @21:41:34.84 SS.hold @21:41:38.27 Intake.select :close @21:41:38.41 SSV.dial :exhaust @21:41:39.12 Exhaust.select :open @21:41:39.23 SS.seek 0ml @21:41:48.54 Exhaust.select :close @21:41:48.64 SSV.dial CBV @21:41:48.67
CBV.dial SSV @21:41:49.40 Allowing 130s for 25.0ml of remaining 450.0ml @21:41:49.41 Intake.select :open @21:41:51.56 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:41:51.58 SS.reconfigure :inhaling 0.46ml/s @21:41:51.62 SS.seek 25ml @21:42:50.40 Stroke completed -- waiting for pressure to equalize ... @21:42:50.41 SS.reconfigure :exhale @21:42:50.45 SS.hold @21:42:56.83 Intake.select :close @21:42:56.96 SSV.dial :exhaust @21:42:57.68 Exhaust.select :open @21:42:57.79 SS.seek 0ml @21:43:07.10 Exhaust.select :close @21:43:07.21 SSV.dial CBV @21:43:07.23
CBV.dial SSV @21:43:07.96 Allowing 130s for 25.0ml of remaining 425.0ml @21:43:07.97 Intake.select :open @21:43:10.12 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:43:10.14 SS.reconfigure :inhaling 0.46ml/s @21:43:10.18 SS.seek 25ml @21:44:10.04 Stroke completed -- waiting for pressure to equalize ... @21:44:10.05 SS.reconfigure :exhale @21:44:10.09 SS.hold @21:44:17.94 Intake.select :close @21:44:18.08 SSV.dial :exhaust @21:44:18.79 Exhaust.select :open @21:44:18.90 SS.seek 0ml @21:44:28.24 Exhaust.select :close @21:44:28.34 SSV.dial CBV @21:44:28.37
CBV.dial SSV @21:44:29.10 Allowing 130s for 25.0ml of remaining 400.0ml @21:44:29.11 Intake.select :open @21:44:31.26 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:44:31.28 SS.reconfigure :inhaling 0.46ml/s @21:44:31.32 SS.seek 25ml @21:45:18.00 Vacuum too high -- waiting up to 40 seconds @21:45:18.01 SS.reconfigure :exhale @21:45:18.05 SS.hold @21:45:22.12 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:45:22.14 SS.reconfigure :inhaling 0.41ml/s @21:45:22.18 SS.seek 25ml @21:45:37.19 Stroke completed -- waiting for pressure to equalize ... @21:45:37.20 SS.reconfigure :exhale @21:45:37.24 SS.hold @21:45:42.78 Intake.select :close @21:45:42.91 SSV.dial :exhaust @21:45:43.65 Exhaust.select :open @21:45:43.75 SS.seek 0ml @21:45:52.75 Exhaust.select :close @21:45:52.86 SSV.dial CBV @21:45:52.88
CBV.dial SSV @21:45:53.64 Allowing 130s for 25.0ml of remaining 375.0ml @21:45:53.65 Intake.select :open @21:45:55.80 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:45:55.82 SS.reconfigure :inhaling 0.41ml/s @21:45:55.86 SS.seek 25ml @21:47:05.05 Stroke completed -- waiting for pressure to equalize ... @21:47:05.06 SS.reconfigure :exhale @21:47:05.10 SS.hold @21:47:12.47 Intake.select :close @21:47:12.61 SSV.dial :exhaust @21:47:13.33 Exhaust.select :open @21:47:13.44 SS.seek 0ml @21:47:22.72 Exhaust.select :close @21:47:22.82 SSV.dial CBV @21:47:22.85
CBV.dial SSV @21:47:23.58 Allowing 130s for 25.0ml of remaining 350.0ml @21:47:23.59 Intake.select :open @21:47:25.74 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:47:25.76 SS.reconfigure :inhaling 0.41ml/s @21:47:25.80 SS.seek 25ml @21:48:34.51 Stroke completed -- waiting for pressure to equalize ... @21:48:34.52 SS.reconfigure :exhale @21:48:34.56 SS.hold @21:48:42.98 Intake.select :close @21:48:43.11 SSV.dial :exhaust @21:48:43.84 Exhaust.select :open @21:48:44.41 SS.seek 0ml @21:48:53.39 Exhaust.select :close @21:48:53.49 SSV.dial CBV @21:48:53.52
CBV.dial SSV @21:48:54.27 Allowing 130s for 25.0ml of remaining 325.0ml @21:48:54.28 Intake.select :open @21:48:56.43 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:48:56.44 SS.reconfigure :inhaling 0.41ml/s @21:48:56.49 SS.seek 25ml @21:49:57.52 Vacuum too high -- waiting up to 40 seconds @21:49:57.53 SS.reconfigure :exhale @21:49:57.57 SS.hold @21:50:03.08 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:50:03.10 SS.reconfigure :inhaling 0.37ml/s @21:50:03.14 SS.seek 25ml @21:50:13.23 Stroke completed -- waiting for pressure to equalize ... @21:50:13.24 SS.reconfigure :exhale @21:50:13.28 SS.hold @21:50:19.69 Intake.select :close @21:50:19.82 SSV.dial :exhaust @21:50:20.54 Exhaust.select :open @21:50:20.65 SS.seek 0ml @21:50:30.04 Exhaust.select :close @21:50:30.14 SSV.dial CBV @21:50:30.17
CBV.dial SSV @21:50:30.90 Allowing 130s for 25.0ml of remaining 300.0ml @21:50:30.91 Intake.select :open @21:50:33.06 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:50:33.08 SS.reconfigure :inhaling 0.37ml/s @21:50:33.12 SS.seek 25ml @21:51:52.55 Stroke completed -- waiting for pressure to equalize ... @21:51:52.56 SS.reconfigure :exhale @21:51:52.60 SS.hold @21:52:02.24 Intake.select :close @21:52:02.38 SSV.dial :exhaust @21:52:03.09 Exhaust.select :open @21:52:03.20 SS.seek 0ml @21:52:12.21 Exhaust.select :close @21:52:12.31 SSV.dial CBV @21:52:12.34
CBV.dial SSV @21:52:13.07 Allowing 130s for 25.0ml of remaining 275.0ml @21:52:13.08 Intake.select :open @21:52:15.23 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:52:15.25 SS.reconfigure :inhaling 0.37ml/s @21:52:15.29 SS.seek 25ml @21:53:37.00 Stroke completed -- waiting for pressure to equalize ... @21:53:37.01 SS.reconfigure :exhale @21:53:37.06 SS.hold @21:53:48.18 Intake.select :close @21:53:48.32 SSV.dial :exhaust @21:53:49.05 Exhaust.select :open @21:53:49.16 SS.seek 0ml @21:53:58.14 Exhaust.select :close @21:53:58.25 SSV.dial CBV @21:53:58.27
CBV.dial SSV @21:53:59.00 Allowing 130s for 25.0ml of remaining 250.0ml @21:53:59.01 Intake.select :open @21:54:01.16 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:54:01.18 SS.reconfigure :inhaling 0.37ml/s @21:54:01.22 SS.seek 25ml @21:55:12.73 Vacuum too high -- waiting up to 40 seconds @21:55:12.74 SS.reconfigure :exhale @21:55:13.24 SS.hold @21:55:19.53 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:55:19.55 SS.reconfigure :inhaling 0.33ml/s @21:55:19.59 SS.seek 25ml @21:55:30.67 Stroke completed -- waiting for pressure to equalize ... @21:55:30.68 SS.reconfigure :exhale @21:55:30.72 SS.hold @21:55:39.14 Intake.select :close @21:55:39.28 SSV.dial :exhaust @21:55:39.98 Exhaust.select :open @21:55:40.09 SS.seek 0ml @21:55:49.51 Exhaust.select :close @21:55:49.62 SSV.dial CBV @21:55:49.64
CBV.dial SSV @21:55:50.38 Allowing 130s for 25.0ml of remaining 225.0ml @21:55:50.39 Intake.select :open @21:55:52.54 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:55:52.55 SS.reconfigure :inhaling 0.33ml/s @21:55:52.60 SS.seek 25ml @21:57:23.49 Stroke completed -- waiting for pressure to equalize ... @21:57:23.50 SS.reconfigure :exhale @21:57:23.55 SS.hold @21:57:34.55 Intake.select :close @21:57:34.68 SSV.dial :exhaust @21:57:35.40 Exhaust.select :open @21:57:35.51 SS.seek 0ml @21:57:44.92 Exhaust.select :close @21:57:45.02 SSV.dial CBV @21:57:45.07
CBV.dial SSV @21:57:45.76 Allowing 130s for 25.0ml of remaining 200.0ml @21:57:45.77 Intake.select :open @21:57:47.92 Intake @15.2psia, vacuum from 9.7 to 12.7psi @21:57:47.94 SS.reconfigure :inhaling 0.33ml/s @21:57:47.98 SS.seek 25ml @21:59:19.19 Stroke completed -- waiting for pressure to equalize ... @21:59:19.20 SS.reconfigure :exhale @21:59:19.24 SS.hold @21:59:31.77 Intake.select :close @21:59:31.91 SSV.dial :exhaust @21:59:32.62 Exhaust.select :open @21:59:32.72 SS.seek 0ml @21:59:41.73 Exhaust.select :close @21:59:41.83 SSV.dial CBV @21:59:41.86
CBV.dial SSV @21:59:42.59 Allowing 130s for 25.0ml of remaining 175.0ml @21:59:42.60 Intake.select :open @21:59:44.75 Intake @15.2psia, vacuum from 9.7 to 12.7psi @21:59:44.76 SS.reconfigure :inhaling 0.33ml/s @21:59:44.81 SS.seek 25ml @22:01:09.09 Vacuum too high -- waiting up to 40 seconds @22:01:09.10 SS.reconfigure :exhale @22:01:09.14 SS.hold @22:01:16.09 Intake @15.4psia, vacuum from 9.9 to 12.9psi @22:01:16.11 SS.reconfigure :inhaling 0.30ml/s @22:01:16.15 SS.seek 25ml @22:01:25.83 Stroke completed -- waiting for pressure to equalize ... @22:01:25.84 SS.reconfigure :exhale @22:01:25.88 SS.hold @22:01:34.47 Intake.select :close @22:01:34.61 SSV.dial :exhaust @22:01:35.33 Exhaust.select :open @22:01:35.44 SS.seek 0ml @22:01:44.83 Exhaust.select :close @22:01:44.93 SSV.dial CBV @22:01:44.96
CBV.dial SSV @22:01:45.69 Allowing 130s for 25.0ml of remaining 150.0ml @22:01:45.70 Intake.select :open @22:01:47.85 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:01:47.87 SS.reconfigure :inhaling 0.30ml/s @22:01:47.91 SS.seek 25ml @22:03:27.92 Stroke completed -- waiting for pressure to equalize ... @22:03:27.93 SS.reconfigure :exhale @22:03:27.97 SS.hold @22:03:42.21 Intake.select :close @22:03:42.35 SSV.dial :exhaust @22:03:43.06 Exhaust.select :open @22:03:43.17 SS.seek 0ml @22:03:52.51 Exhaust.select :close @22:03:52.62 SSV.dial CBV @22:03:52.64
CBV.dial SSV @22:03:53.38 Allowing 130s for 25.0ml of remaining 125.0ml @22:03:53.39 Intake.select :open @22:03:55.54 Intake @15.1psia, vacuum from 9.6 to 12.6psi @22:03:55.56 SS.reconfigure :inhaling 0.30ml/s @22:03:55.60 SS.seek 25ml @22:05:34.82 Stroke completed -- waiting for pressure to equalize ... @22:05:34.83 SS.reconfigure :exhale @22:05:34.87 SS.hold @22:05:51.03 Intake.select :close @22:05:51.17 SSV.dial :exhaust @22:05:51.89 Exhaust.select :open @22:05:51.99 SS.seek 0ml @22:06:01.01 Exhaust.select :close @22:06:01.11 SSV.dial CBV @22:06:01.14
CBV.dial SSV @22:06:01.89 Allowing 130s for 25.0ml of remaining 100.0ml @22:06:01.90 Intake.select :open @22:06:04.05 Intake @15.1psia, vacuum from 9.6 to 12.6psi @22:06:04.06 SS.reconfigure :inhaling 0.30ml/s @22:06:04.11 SS.seek 25ml @22:07:20.92 Vacuum too high -- waiting up to 40 seconds @22:07:20.93 SS.reconfigure :exhale @22:07:20.97 SS.hold @22:07:27.57 Intake @14.6psia, vacuum from 9.1 to 12.1psi @22:07:27.59 SS.reconfigure :inhaling 0.27ml/s @22:07:27.63 SS.seek 25ml @22:07:54.54 Stroke completed -- waiting for pressure to equalize ... @22:07:54.55 SS.reconfigure :exhale @22:07:54.59 SS.hold @22:08:08.83 Intake.select :close @22:08:08.97 SSV.dial :exhaust @22:08:09.69 Exhaust.select :open @22:08:09.80 SS.seek 0ml @22:08:18.77 Exhaust.select :close @22:08:18.87 SSV.dial CBV @22:08:18.90
CBV.dial SSV @22:08:19.63 Allowing 130s for 25.0ml of remaining 75.0ml @22:08:19.64 Intake.select :open @22:08:21.79 Intake @15.2psia, vacuum from 9.7 to 12.7psi @22:08:21.81 SS.reconfigure :inhaling 0.27ml/s @22:08:21.85 SS.seek 25ml @22:10:11.36 Stroke completed -- waiting for pressure to equalize ... @22:10:11.37 SS.reconfigure :exhale @22:10:11.41 SS.hold @22:10:29.61 Intake.select :close @22:10:29.75 SSV.dial :exhaust @22:10:30.45 Exhaust.select :open @22:10:30.56 SS.seek 0ml @22:10:39.91 Exhaust.select :close @22:10:40.01 SSV.dial CBV @22:10:40.06
CBV.dial SSV @22:10:40.75 Allowing 130s for 25.0ml of remaining 50.0ml @22:10:40.76 Intake.select :open @22:10:42.91 Intake @15.3psia, vacuum from 9.8 to 12.8psi @22:10:42.93 SS.reconfigure :inhaling 0.27ml/s @22:10:42.97 SS.seek 25ml @22:12:15.66 Vacuum too high -- waiting up to 40 seconds @22:12:15.67 SS.reconfigure :exhale @22:12:15.71 SS.hold @22:12:24.23 SS.reconfigure :inhaling 0.24ml/s @22:12:24.27 SS.seek 25ml @22:12:44.01 Stroke completed -- waiting for pressure to equalize ... @22:12:44.02 SS.reconfigure :exhale @22:12:44.06 SS.hold @22:12:50.78 SS.reconfigure :default @22:12:50.89 SS.hold @22:12:50.99 Intake.select :close @22:12:51.61 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 975.0ml @22:12:51.62 SSV.dial :exhaust @22:12:52.36 Exhaust.select :open @22:12:52.47 SS.reconfigure :exhale @22:12:52.51 SS.seek 0ml @22:13:01.90 Exhaust.select :close @22:13:02.00 Sampled 975.0ml @22:13:02.02 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @22:13:02.03 Intake.select :close @22:13:02.09 CSV.dial :waste @22:13:02.13 CS.seek 0ml @22:13:02.23 SSV.dial CBV,avoiding: :exhaust @22:13:02.25
CBV.dial SSV @22:13:02.31 CSV.dial :air @22:13:03.03 CS.seek 10ml @22:13:04.06
CTV.dial :air @22:13:04.80 delay 15 seconds @22:13:19.82 SSV.dial :spit,avoiding: :exhaust @22:13:21.10 CTV.dial CSV,avoiding: :intake @22:13:21.13
SS.seek 0ml @22:13:21.25 SSV.dial CBV,avoiding: :exhaust @22:13:25.79 CBV.dial between SSV and waste2 @22:13:26.16 CTV.dial CSV,avoiding: :air @22:13:26.18
CSV.dial CTV @22:13:26.62 CS.reconfigure :pressurize @22:13:26.66 CS.seek 0ml @22:13:44.56 BubblePoint: Intake @40.5psia, Outlet @16.3psia @22:13:44.57 CBV.dial SSV @22:13:44.90 SS.reconfigure :default @22:13:44.94 SS.seek 10ml @22:13:50.35 delay 15 seconds @22:14:05.47 Evacuated: Intake @16.4psia, Outlet @10.3psia @22:14:05.48 SSV.dial :spit,avoiding: :exhaust @22:14:06.78 SS.reconfigure :exhale @22:14:06.83 SS.seek 0ml @22:14:12.58 SS.reconfigure :fast @22:14:12.62 SS.seek 5ml @22:14:15.22 SSV.dial CBV,avoiding: :exhaust @22:14:15.24
CBV.dial SSV @22:14:16.53 CTV.dial :air,avoiding: :intake @22:14:17.79 CSV.dial CTV @22:14:17.82
SS.seek 10ml @22:14:18.82 CSV.dial :air @22:14:19.26 CS.reconfigure :fast @22:14:19.30 CS.seek 0ml @22:14:20.29
SSV.dial :spit,avoiding: :exhaust @22:14:21.59 SS.reconfigure :exhale @22:14:21.65 SS.seek 0ml @22:14:26.90 CS.stop @22:14:26.95
SS.reconfigure :fast @22:14:26.99 SS.seek 5ml @22:14:29.81 SSV.dial CBV,avoiding: :exhaust @22:14:29.84
CBV.dial SSV @22:14:32.13 SSV.dial :spit,avoiding: :exhaust @22:14:33.41 SS.reconfigure :default @22:14:33.45 SS.seek 0ml @22:14:36.77 SS.stop @22:14:36.81 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @22:14:36.82 Intake.select :close @22:14:36.89 CSV.dial :waste @22:14:37.00 SSV.dial CBV,avoiding: :exhaust @22:14:37.02
CBV.dial SSV @22:14:37.65 CS.seek 0ml @22:14:37.72 CSV.dial :air @22:14:38.29
CTV.dial :air @22:14:38.31 delay 15 seconds @22:14:38.43 CS.seek 10ml @22:14:53.32
SSV.dial :spit,avoiding: :exhaust @22:14:54.61 CTV.dial CSV,avoiding: :intake @22:14:54.64
SS.reconfigure :exhale @22:14:54.74 SS.seek 0ml @22:14:55.21 SSV.dial CBV,avoiding: :exhaust @22:15:01.02 CBV.dial between SSV and waste2 @22:15:01.40 CTV.dial CSV,avoiding: :air @22:15:01.43
CSV.dial CTV @22:15:01.87 CS.reconfigure :pressurize @22:15:01.91 CS.seek 0ml @22:15:23.14 BubblePoint: Intake @39.6psia, Outlet @15.4psia @22:15:23.15 CBV.dial SSV @22:15:23.47 SS.reconfigure :default @22:15:23.52 SS.seek 10ml @22:15:29.13 delay 15 seconds @22:15:44.24 Evacuated: Intake @18.8psia, Outlet @12.6psia @22:15:44.26 SSV.dial :spit,avoiding: :exhaust @22:15:45.53 SS.reconfigure :exhale @22:15:45.57 SS.seek 0ml @22:15:51.03 SS.reconfigure :fast @22:15:51.07 SS.seek 5ml @22:15:54.07 SSV.dial CBV,avoiding: :exhaust @22:15:54.10
CBV.dial SSV @22:15:55.82 CTV.dial :air,avoiding: :intake @22:15:57.06 CSV.dial CTV @22:15:57.10
SS.seek 10ml @22:15:58.10 CSV.dial :air @22:15:58.66 CS.reconfigure :fast @22:15:58.70 CS.seek 0ml @22:15:59.77
SSV.dial :spit,avoiding: :exhaust @22:16:01.07 SS.reconfigure :exhale @22:16:01.11 SS.seek 0ml @22:16:03.47 CS.stop @22:16:06.39
SS.reconfigure :fast @22:16:06.44 SS.seek 5ml @22:16:09.17 SSV.dial CBV,avoiding: :exhaust @22:16:09.20
CBV.dial SSV @22:16:11.49 SSV.dial :spit,avoiding: :exhaust @22:16:12.78 SS.reconfigure :default @22:16:12.82 SS.seek 0ml @22:16:16.44 SS.stop @22:16:16.48 Lysing: Adding 2.0ml AqMeOH @22:16:16.50 CBV.dial between CSV and waste1,avoiding: :DBV @22:16:17.94 CTV.dial CSV @22:16:19.19 CSV.dial CSR @22:16:19.92 Pulling 1ml of CSR.AqMeOH @22:16:19.93 CSR.select :AqMeOH @22:16:20.04 CS.reconfigure :default @22:16:20.08 CS.seek 1ml @22:16:23.44 delay 5 seconds @22:16:28.45 CSR.select :air @22:16:28.61 CS.seek 6ml @22:16:42.03 delay 2 seconds @22:16:44.04 CSV.dial :waste2 @22:16:44.49 CS.reconfigure :fast @22:16:44.53 CS.seek 0ml @22:16:58.31 CSV.dial CSR @22:16:58.91 Pulling 2ml of CSR.AqMeOH @22:16:58.92 CSR.select :AqMeOH @22:16:59.02 CS.reconfigure :default @22:16:59.06 CS.seek 2ml @22:17:04.94 delay 5 seconds @22:17:09.95 CSR.select :air @22:17:10.10 CS.seek 5ml @22:17:18.43 delay 2 seconds @22:17:20.45 CSV.dial CTV @22:17:20.89 CS.seek 1ml @22:17:31.94 Before lysing Collection Puck Heater at 18.9C/38.7psia @22:17:31.96 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @22:17:31.98 CH.seek 70C,8:00 @22:19:17.69 CH.hold 10:00,70C @22:19:17.86 Begun lysing Collection Puck Heater at 68C/42.5psia @22:29:17.88 Ended lysing Collection Puck Heater at 70C/44.0psia @22:29:17.89 CS.seek 0ml @22:29:21.26 CTV.dial between spare and air,avoiding: :intake @22:29:22.73 CSV.dial :air @22:29:23.28 CS.seek 5ml @22:29:36.72 CSV.dial CTV @22:29:37.13 delay 2 seconds @22:29:39.15 CSV.dial :air @22:29:39.71 delay 2 seconds @22:29:41.73 CSV.dial CTV @22:29:42.15 delay 2 seconds @22:29:44.16 CSV.dial :air @22:29:44.73 delay 2 seconds @22:29:46.75 CS.seek 0ml @22:30:00.31 Puck @70.3psia after final lysis pressurization @22:30:00.32 Recovering Lysate into Collection syringe @22:30:00.33 CSV.dial CBV @22:30:01.32 CBV.dial CSV @22:30:01.67 CS.seek 5ml @22:30:15.07 delay 5 seconds @22:30:20.09 CTV.dial :air @22:30:20.60 CS.seek 6.999ml @22:30:26.47 delay 5 seconds @22:30:31.49 CSV.dial :air2 @22:30:31.93 CS.seek 10ml @22:30:40.32 CSV.dial between air and CTV,avoiding: CBV @22:30:41.43 Lysing Complete @22:30:41.50 Storing sh1 puck in Collection Clamp to tube 1 @22:30:41.65 SC.reconfigure :tight @22:30:41.70 SC.seek 1 @22:30:45.31 SC.configure :limp @22:30:45.50 Elbow.reconfigure :tight @22:30:45.55 Elbow.seek 1 @22:30:47.43 Forearm.reconfigure :tight @22:30:47.47 Forearm.seek 1 + 700 counts @22:30:48.45 SE.reconfigure :pick @22:30:48.50 SE.seek :beamBreak @22:30:50.06 SE.configure :default @22:30:50.20 Elbow.reconfigure :limp @22:30:50.25 Forearm.reconfigure :loose @22:30:50.28 Forearm.seek 1 @22:30:50.69 Elbow.hold @22:30:50.76 Elbow.reconfigure :tight @22:30:50.84 SE.seek 23.37 Pucks below Top Plate @22:30:51.33 Forearm.force -20 @22:30:51.37 Forearm.seek 1 + 320 counts @22:30:51.73 Forearm.reconfigure :tight @22:30:51.77 Forearm.seek 1 + 700 counts @22:30:51.96 SE.seek 24.37 Pucks below Top Plate @22:30:52.55 Forearm.reconfigure :loose @22:30:52.59 Forearm.seek :retract @22:30:53.59 Elbow.seek CC @22:30:55.10 Elbow.reconfigure :loose @22:30:55.19 Forearm.seek CC @22:30:56.14 Hand.close @22:30:56.73 CC.seek :open @22:31:01.16 Forearm.seek :retract @22:31:02.24 Elbow.reconfigure :tight @22:31:02.28 Elbow.seek 1 @22:31:04.06 Elbow.reconfigure :limp @22:31:04.11 Forearm.seek 1 @22:31:05.21 Elbow.hold @22:31:05.27 Elbow.reconfigure :tight @22:31:05.31 Hand.open @22:31:06.21 Forearm.force -20 @22:31:06.24 Forearm.seek 1 + 319 counts @22:31:06.55 Forearm.reconfigure :tight @22:31:06.59 Forearm.seek 1 + 700 counts @22:31:06.99 SE.seek :down @22:31:08.07 SE.stop @22:31:08.18
Forearm.reconfigure :loose @22:31:08.23 Forearm.seek :retract @22:31:09.21 Tranferring DA @22:31:09.22 Beginning samplePassC @22:31:09.23 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:31:09.41 Elbow.seek FlushPuck::Garage @22:31:11.22 Elbow.reconfigure :loose @22:31:11.31 Forearm.seek :garage @22:31:12.86 Hand.close @22:31:13.58 Forearm.seek :retract @22:31:14.63 Elbow.reconfigure :tight @22:31:14.68 Elbow.seek CC @22:31:17.53 Elbow.reconfigure :loose @22:31:17.61 Forearm.reconfigure :tight @22:31:17.66 Forearm.seek CC @22:31:18.90 CC.seek :closed @22:31:23.17 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.96 @22:31:23.24 Hand.open @22:31:24.03 Forearm.reconfigure :loose @22:31:24.08 Forearm.seek :retract @22:31:25.11 PSV.dial PSR @22:31:26.40 Pulling 1.5ml of PSR.flush @22:31:26.41 PSR.select :flush @22:31:26.53 PS.seek 1.5ml @22:31:42.62 delay 5 seconds @22:31:47.64 PSV.dial :waste1 @22:31:48.07 PS.seek 0ml @22:32:04.08 PSV.dial PSR @22:32:04.65 PSR.select :air @22:32:04.76 PS.seek 2.5ml @22:32:30.96 PRV.dial CBV,avoiding: :ab2 @22:32:31.00
CBV.dial PRV @22:32:32.61 PSV.dial :mixloop @22:32:33.60 CTV.dial :air,avoiding: :intake @22:32:33.61 PTV.dial :PRVmixing @22:32:34.84 PS.seek 0ml @22:33:01.08 PSV.dial :air @22:33:01.51 PS.seek 2.5ml @22:33:27.70 PSV.dial :mixloop @22:33:28.15 PS.seek 0ml @22:33:54.27 PSV.dial :air @22:33:54.71 PS.seek 2.5ml @22:34:20.86 PSV.dial :mixloop @22:34:21.31 PS.seek 0ml @22:34:47.49 Garaging FlushPuck in Collection Clamp @22:34:47.55 Elbow.reconfigure :tight @22:34:47.60 Elbow.seek CC @22:34:47.71 Elbow.reconfigure :loose @22:34:47.79 Forearm.seek CC @22:34:48.71 Hand.close @22:34:49.34 CC.seek :open @22:34:53.67 Forearm.seek :retract @22:34:54.75 Elbow.reconfigure :tight @22:34:54.79 Elbow.seek :garage @22:34:57.89 Elbow.reconfigure :loose @22:34:57.98 Forearm.reconfigure :tight @22:34:58.02 Forearm.seek :garage @22:34:59.06 Hand.open @22:34:59.90 Forearm.reconfigure :loose @22:34:59.94 Forearm.seek :retract @22:35:00.98 PSV.dial :mixloop @22:35:00.99 PTV.dial :PRVmixing @22:35:01.00 PRV.dial CBV @22:35:01.03
CBV.dial PRV @22:35:01.04 CTV.dial :air,avoiding: :intake @22:35:01.06 Loading lyfil puck from tube 2 into Collection Clamp @22:35:01.27 SC.reconfigure :tight @22:35:01.31 SC.seek 2 @22:35:04.89 SC.configure :limp @22:35:05.09 Elbow.reconfigure :tight @22:35:05.13 Elbow.seek 2 @22:35:07.06 Forearm.reconfigure :tight @22:35:07.10 Forearm.seek 2 + 700 counts @22:35:08.04 SE.reconfigure :pick @22:35:08.09 SE.seek :beamBreak @22:35:11.22 SE.configure :default @22:35:11.79 Elbow.seek 2 @22:35:11.96 Forearm.seek 2 + 300 counts @22:35:12.21 Forearm.reconfigure :loose @22:35:12.26 Hand.close @22:35:12.85 SE.seek :down @22:35:13.04
Forearm.seek :retract @22:35:14.06 Elbow.seek CC @22:35:15.27 SE.stop @22:35:15.61
Elbow.reconfigure :loose @22:35:15.71 Forearm.reconfigure :tight @22:35:15.76 Forearm.seek CC @22:35:17.06 CC.seek :closed @22:35:21.30 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.69 @22:35:21.31 Hand.open @22:35:22.15 Forearm.reconfigure :loose @22:35:22.19 Forearm.seek :retract @22:35:23.23 CTV.dial CSV,avoiding: :intake @22:35:24.50 CSV.dial CTV @22:35:24.80 PS.seek 2.5ml @22:35:51.07 CS.seek 5.5ml @22:36:03.21 delay 8 seconds @22:36:11.24 PSV.dial :air @22:36:11.69 delay 2 seconds @22:36:13.70 PSV.dial :waste2 @22:36:14.40 PS.seek 0ml @22:36:40.64 CBV.dial between PRV and waste1 @22:36:40.95 CSV.dial :air @22:36:41.41 CS.seek 10ml @22:36:53.56 CSV.dial CTV @22:36:54.11 CS.seek 0ml @22:37:20.18 CBV.dial :waste1 @22:37:20.48 delay 5 seconds @22:37:25.50 CTV.dial :air,avoiding: :intake @22:37:26.80 Storing lyfil puck in Collection Clamp to tube 1 @22:37:26.95 SC.reconfigure :tight @22:37:27.00 SC.seek 1 @22:37:30.60 SC.configure :limp @22:37:30.79 Elbow.reconfigure :tight @22:37:30.83 Elbow.seek 1 @22:37:32.49 Forearm.reconfigure :tight @22:37:32.53 Forearm.seek 1 + 700 counts @22:37:33.60 SE.reconfigure :pick @22:37:33.64 SE.seek :beamBreak @22:37:35.14 SE.configure :default @22:37:35.29 Elbow.reconfigure :limp @22:37:35.34 Forearm.reconfigure :loose @22:37:35.38 Forearm.seek 1 @22:37:35.79 Elbow.hold @22:37:35.84 Elbow.reconfigure :tight @22:37:35.93 SE.seek 24.38 Pucks below Top Plate @22:37:36.42 Forearm.force -20 @22:37:36.45 Forearm.seek 1 + 319 counts @22:37:36.75 Forearm.reconfigure :tight @22:37:36.79 Forearm.seek 1 + 700 counts @22:37:37.01 SE.seek 25.38 Pucks below Top Plate @22:37:37.66 Forearm.reconfigure :loose @22:37:37.70 Forearm.seek :retract @22:37:38.59 Elbow.seek CC @22:37:40.22 Elbow.reconfigure :loose @22:37:40.30 Forearm.seek CC @22:37:41.32 Hand.close @22:37:42.46 CC.seek :open @22:37:46.90 Forearm.seek :retract @22:37:47.95 Elbow.reconfigure :tight @22:37:47.99 Elbow.seek 1 @22:37:49.75 Elbow.reconfigure :limp @22:37:49.80 Forearm.seek 1 @22:37:50.86 Elbow.hold @22:37:50.92 Elbow.reconfigure :tight @22:37:50.97 Hand.open @22:37:51.83 Forearm.force -20 @22:37:51.86 Forearm.seek 1 + 316 counts @22:37:52.18 Forearm.reconfigure :tight @22:37:52.22 Forearm.seek 1 + 700 counts @22:37:52.63 SE.seek :down @22:37:53.71 SE.stop @22:37:53.82
Forearm.reconfigure :loose @22:37:53.86 Forearm.seek :retract @22:37:54.79 PSV.dial :air @22:37:55.61 PS.seek 2.5ml @22:38:21.76 delay 1 second @22:38:22.77 PSV.dial :mixloop @22:38:23.21 delay 1 second @22:38:24.23 PSV.dial :air @22:38:24.67 delay 1 second @22:38:25.68 PSV.dial :mixloop @22:38:26.12 delay 1 second @22:38:27.13 PSV.dial :air @22:38:27.57 delay 1 second @22:38:28.59 PS.seek 0ml @22:38:54.73 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:38:54.90 Elbow.seek FlushPuck::Garage @22:38:56.65 Elbow.reconfigure :loose @22:38:56.74 Forearm.seek :garage @22:38:57.86 Hand.close @22:38:58.56 Forearm.seek :retract @22:38:59.69 Elbow.reconfigure :tight @22:38:59.74 Elbow.seek PC @22:39:01.23 Elbow.reconfigure :loose @22:39:01.32 Forearm.reconfigure :tight @22:39:01.36 Forearm.seek PC @22:39:02.69 PC.seek :closed @22:39:06.57 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.43 @22:39:06.64 Hand.open @22:39:07.26 Forearm.reconfigure :loose @22:39:07.30 Forearm.seek :retract @22:39:08.30 PRV.dial :air @22:39:08.73 PSV.dial :mixloop @22:39:09.23 PS.seek 0.3ml @22:39:13.07 PSV.dial PSR @22:39:14.08 Pulling 0.4ml of PSR.air PSR.select :air @22:39:14.11 PS.seek 0.7ml @22:39:18.95 delay 3 seconds @22:39:21.96 PSR.select :air @22:39:21.98 PS.seek 2.5ml @22:39:40.98 delay 3 seconds @22:39:43.99 PSV.dial :mixloop @22:39:44.96 PTV.dial :mixing @22:39:45.65 PBV.dial :waste2 @22:39:46.51 PS.seek 0ml @22:40:12.66 Garaging FlushPuck in Processing Clamp @22:40:12.73 Elbow.reconfigure :tight @22:40:12.78 Elbow.seek PC @22:40:12.88 Elbow.reconfigure :loose @22:40:12.96 Forearm.seek PC @22:40:13.92 Hand.close @22:40:14.58 PC.seek :open @22:40:18.99 Forearm.seek :retract @22:40:20.07 Elbow.reconfigure :tight @22:40:20.11 Elbow.seek :garage @22:40:21.44 Elbow.reconfigure :loose @22:40:21.53 Forearm.reconfigure :tight @22:40:21.58 Forearm.seek :garage @22:40:22.72 Hand.open @22:40:23.52 Forearm.reconfigure :loose @22:40:23.56 Forearm.seek :retract @22:40:24.61 Loading da puck from tube 2 into Processing Clamp @22:40:24.83 SC.reconfigure :tight @22:40:24.87 SC.seek 2 @22:40:28.50 SC.configure :limp @22:40:28.69 Elbow.reconfigure :tight @22:40:28.73 Elbow.seek 2 @22:40:30.68 Forearm.reconfigure :tight @22:40:30.72 Forearm.seek 2 + 700 counts @22:40:31.68 SE.reconfigure :pick @22:40:31.73 SE.seek :beamBreak @22:40:34.36 SE.configure :default @22:40:34.50 Elbow.seek 2 @22:40:34.83 Forearm.seek 2 + 300 counts @22:40:35.08 Forearm.reconfigure :loose @22:40:35.12 Hand.close @22:40:35.72 SE.seek :down @22:40:35.90
Forearm.seek :retract @22:40:36.85 Elbow.seek PC @22:40:38.07 SE.stop @22:40:39.35
Elbow.reconfigure :loose @22:40:39.45 Forearm.reconfigure :tight @22:40:39.49 Forearm.seek PC @22:40:40.85 PC.seek :closed @22:40:44.72 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.41 @22:40:44.73 Hand.open @22:40:45.38 Forearm.reconfigure :loose @22:40:45.43 Forearm.seek :retract @22:40:46.45 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @22:40:46.46 PH.seek 28C,5:00 @22:41:35.67 PH.hold 2:00:00,28C @22:41:35.73 delay 1:30 @22:43:05.75 PSR.select :air @22:43:05.76 PSV.dial PSR @22:43:06.79 Pulling 0.5ml of PSR.wash @22:43:06.80 PSR.select :wash @22:43:06.91 PS.seek 0.5ml @22:43:12.85 delay 5 seconds @22:43:17.87 PSR.select :air @22:43:17.98 PS.seek 2ml @22:43:34.00 PTV.dial :puck @22:43:34.67 PSV.dial PTV @22:43:35.38 PBV.dial :waste1 @22:43:36.69 PS.seek 1.5ml @22:43:42.69 delay 3 seconds @22:43:45.70 PBV.dial between PSV and waste1 @22:43:46.06 PS.seek 0ml @22:44:02.16 delay 5:00 @22:49:02.18 recoverPSRreagent08 @22:49:02.19 PTV.dial :puck @22:49:02.60 PBV.dial :waste1 @22:49:03.04 delay 30 seconds @22:49:33.06 PBV.dial between PSV and waste1 @22:49:33.41 PSR.select :air @22:49:33.43 PSV.dial PSR @22:49:34.15 PS.reconfigure :fast @22:49:34.20 PS.seek 2.5ml @22:49:56.83 PSV.dial PTV @22:49:57.65 delay 5 seconds @22:50:02.67 PSV.dial PSR @22:50:03.48 delay 5 seconds @22:50:08.49 PSV.dial PTV @22:50:09.18 delay 5 seconds @22:50:14.20 PSV.dial :air @22:50:14.92 PS.seek 0ml @22:50:37.46 PTV.dial :PRVmixing,via: PRV @22:50:38.72 PSV.dial :mixloop @22:50:39.16 PRV.dial :ab1 @22:50:39.89 PS.reconfigure :slow @22:50:39.93 PS.seek 0.15ml @22:50:43.55 delay 2 seconds @22:50:45.57 CBV.dial PRV @22:50:45.62
PRV.dial CBV @22:50:46.14 PS.seek 0.34ml @22:50:50.46 delay 2 seconds @22:50:52.47 CBV.dial between PRV and PSV @22:50:52.77 PRV.dial :air @22:50:53.20 PS.seek 0.9ml @22:51:05.02 PRV.dial :waste2 @22:51:05.73 PS.seek 0.5ml @22:51:14.39 addPMLReagent08 @22:51:14.40 PSV.dial :air @22:51:14.85 PS.reconfigure :default @22:51:14.90 PS.seek 2ml @22:51:31.11 PSV.dial :PML @22:51:31.55 PTV.dial :mixing @22:51:32.25 PBV.dial :waste1 @22:51:32.66 PS.seek 1.85ml @22:51:35.05 PBV.dial between PSV and waste1 @22:51:35.42 PS.seek 0ml @22:51:55.02 delay 2 seconds @22:51:57.04 Beginning samplePassC @22:51:57.05 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:51:57.21 Elbow.reconfigure :tight @22:51:57.25 Elbow.seek FlushPuck::Garage @22:51:58.59 Elbow.reconfigure :loose @22:51:58.68 Forearm.seek :garage @22:51:59.79 Hand.close @22:52:00.55 Forearm.seek :retract @22:52:01.57 Elbow.reconfigure :tight @22:52:01.61 Elbow.seek CC @22:52:04.37 Elbow.reconfigure :loose @22:52:04.46 Forearm.reconfigure :tight @22:52:04.50 Forearm.seek CC @22:52:05.75 CC.seek :closed @22:52:09.95 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.84 @22:52:10.02 Hand.open @22:52:10.86 Forearm.reconfigure :loose @22:52:10.91 Forearm.seek :retract @22:52:11.90 PSV.dial PSR @22:52:12.93 Pulling 1.5ml of PSR.flush @22:52:12.94 PSR.select :flush @22:52:13.06 PS.seek 1.5ml @22:52:29.02 delay 5 seconds @22:52:34.04 PSV.dial :waste1 @22:52:34.48 PS.seek 0ml @22:52:50.52 PSV.dial PSR @22:52:51.06 PSR.select :air @22:52:51.17 PS.seek 2.5ml @22:53:17.43 PRV.dial CBV,avoiding: :ab2 @22:53:17.45
CBV.dial PRV @22:53:19.02 PSV.dial :mixloop @22:53:20.02 CTV.dial :air,avoiding: :intake @22:53:20.03 PTV.dial :PRVmixing @22:53:21.21 PS.seek 0ml @22:53:47.45 PSV.dial :air @22:53:47.88 PS.seek 2.5ml @22:54:14.14 PSV.dial :mixloop @22:54:14.58 PS.seek 0ml @22:54:40.70 PSV.dial :air @22:54:41.15 PS.seek 2.5ml @22:55:07.26 PSV.dial :mixloop @22:55:07.71 PS.seek 0ml @22:55:33.89 Garaging FlushPuck in Collection Clamp @22:55:33.96 Elbow.reconfigure :tight @22:55:34.00 Elbow.seek CC @22:55:34.12 Elbow.reconfigure :loose @22:55:34.21 Forearm.seek CC @22:55:35.19 Hand.close @22:55:35.80 CC.seek :open @22:55:40.14 Forearm.seek :retract @22:55:41.13 Elbow.reconfigure :tight @22:55:41.17 Elbow.seek :garage @22:55:44.06 Elbow.reconfigure :loose @22:55:44.14 Forearm.reconfigure :tight @22:55:44.19 Forearm.seek :garage @22:55:45.32 Hand.open @22:55:46.11 Forearm.reconfigure :loose @22:55:46.15 Forearm.seek :retract @22:55:47.16 delayUntil "22:59:57.02" @22:55:47.18 Whole Cell Archive sampling 1000ml @22:55:47.20 Loading wcr puck from tube 2 into Collection Clamp @22:55:47.42 SC.reconfigure :tight @22:55:47.46 SC.seek 2 @22:55:47.57 SC.configure :limp @22:55:47.77 Elbow.reconfigure :tight @22:55:47.82 Elbow.seek 2 @22:55:49.80 Forearm.reconfigure :tight @22:55:49.85 Forearm.seek 2 + 700 counts @22:55:50.80 SE.reconfigure :pick @22:55:50.86 SE.seek :beamBreak @22:55:53.59 SE.configure :default @22:55:53.73 Elbow.seek 2 @22:55:53.91 Forearm.seek 2 + 300 counts @22:55:54.17 Forearm.reconfigure :loose @22:55:54.21 Hand.close @22:55:54.81 SE.seek :down @22:55:55.01 Forearm.seek :retract @22:55:56.03 Elbow.seek CC @22:55:57.32 SE.stop @22:55:57.59 Elbow.reconfigure :loose @22:55:57.68 Forearm.reconfigure :tight @22:55:57.73 Forearm.seek CC @22:55:59.05 CC.seek :closed @22:56:03.30 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.73 @22:56:03.31 Hand.open @22:56:04.10 Forearm.reconfigure :loose @22:56:04.15 Forearm.seek :retract @22:56:05.14 collectSample 1000ml w/bubble point of 35psi @22:56:05.16 Priming 7ml Intake.select :close @22:56:05.17 Exhaust.select :close @22:56:05.18 SS.hold @22:56:05.37 Calibrating pressure sensors @22:56:05.38 CTV.dial :air @22:56:05.40 SSV.dial :spit,avoiding: :exhaust @22:56:05.87 SS.reconfigure :fast @22:56:05.92 SS.seek 5ml @22:56:08.65 SSV.dial CBV @22:56:08.69 CBV.dial SSV @22:56:09.96 delay 3.8 seconds @22:56:13.88 SSV.dial :spit @22:56:15.17 SS.seek 0ml @22:56:17.94 SSV.dial CBV @22:56:19.22 CTV.dial :intake @22:56:19.95 SSV.dial CBV @22:56:19.97 CBV.dial SSV @22:56:19.99 Allowing 40s for 7.0ml of remaining 7.0ml @22:56:20.00 Intake.select :open @22:56:22.15 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:56:22.17 SS.reconfigure :inhaling 2.14ml/s @22:56:22.22 SS.seek 7ml @22:56:28.78 Stroke completed -- waiting for pressure to equalize ... @22:56:28.80 SS.reconfigure :exhale @22:56:28.84 SS.hold @22:56:36.20 Intake.select :close @22:56:36.34 SSV.dial :exhaust @22:56:37.07 Exhaust.select :open @22:56:37.18 SS.seek 0ml @22:56:41.66 Exhaust.select :close @22:56:41.77 Sampling 1000.0ml @22:56:41.78 Intake.select :close @22:56:41.79 Exhaust.select :close @22:56:41.80 SS.reconfigure :default @22:56:41.84 SS.hold @22:56:42.03 CTV.dial :intake @22:56:42.05 SSV.dial CBV @22:56:42.07 CBV.dial SSV @22:56:42.80 Allowing 130s for 25.0ml of remaining 1000.0ml @22:56:42.82 Intake.select :open @22:56:44.97 Intake @15.2psia, vacuum from 9.7 to 12.7psi @22:56:44.99 SS.reconfigure :inhaling 2.14ml/s @22:56:45.03 SS.seek 25ml @22:56:49.29 Vacuum too high -- waiting up to 40 seconds @22:56:49.30 SS.reconfigure :exhale @22:56:49.35 SS.hold @22:56:51.68 Intake @14.6psia, vacuum from 9.1 to 12.1psi @22:56:51.70 SS.reconfigure :inhaling 1.93ml/s @22:56:51.74 SS.seek 25ml @22:56:54.33 Vacuum too high -- waiting up to 40 seconds @22:56:54.34 SS.reconfigure :exhale @22:56:54.39 SS.hold @22:56:56.35 Intake @15.0psia, vacuum from 9.5 to 12.5psi @22:56:56.37 SS.reconfigure :inhaling 1.74ml/s @22:56:56.41 SS.seek 25ml @22:56:58.95 Vacuum too high -- waiting up to 40 seconds @22:56:58.96 SS.reconfigure :exhale @22:56:59.00 SS.hold @22:57:00.81 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:57:00.83 SS.reconfigure :inhaling 1.57ml/s @22:57:00.88 SS.seek 25ml @22:57:03.35 Vacuum too high -- waiting up to 40 seconds @22:57:03.37 SS.reconfigure :exhale @22:57:03.41 SS.hold @22:57:05.21 Intake @14.9psia, vacuum from 9.4 to 12.4psi @22:57:05.22 SS.reconfigure :inhaling 1.41ml/s @22:57:05.27 SS.seek 25ml @22:57:08.48 Vacuum too high -- waiting up to 40 seconds @22:57:08.49 SS.reconfigure :exhale @22:57:08.53 SS.hold @22:57:10.61 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:57:10.63 SS.reconfigure :inhaling 1.27ml/s @22:57:10.67 SS.seek 25ml @22:57:14.64 Vacuum too high -- waiting up to 40 seconds @22:57:14.66 SS.reconfigure :exhale @22:57:14.70 SS.hold @22:57:16.74 Intake @14.7psia, vacuum from 9.2 to 12.2psi @22:57:16.76 SS.reconfigure :inhaling 1.14ml/s @22:57:16.81 SS.seek 25ml @22:57:20.36 Stroke completed -- waiting for pressure to equalize ... @22:57:20.37 SS.reconfigure :exhale @22:57:20.41 SS.hold @22:57:23.40 Intake.select :close @22:57:23.54 SSV.dial :exhaust @22:57:24.27 Exhaust.select :open @22:57:24.38 SS.seek 0ml @22:57:33.33 Exhaust.select :close @22:57:33.44 SSV.dial CBV @22:57:33.46 CBV.dial SSV @22:57:34.21 Allowing 130s for 25.0ml of remaining 975.0ml @22:57:34.22 Intake.select :open @22:57:36.37 Intake @14.9psia, vacuum from 9.4 to 12.4psi @22:57:36.39 SS.reconfigure :inhaling 1.14ml/s @22:57:36.43 SS.seek 25ml @22:57:41.76 Vacuum too high -- waiting up to 40 seconds @22:57:41.77 SS.reconfigure :exhale @22:57:41.81 SS.hold @22:57:42.81 Intake @14.1psia, vacuum from 8.6 to 11.6psi @22:57:42.82 SS.reconfigure :inhaling 1.03ml/s @22:57:42.87 SS.seek 25ml @22:57:47.73 Vacuum too high -- waiting up to 40 seconds @22:57:47.75 SS.reconfigure :exhale @22:57:47.79 SS.hold @22:57:49.43 Intake @14.7psia, vacuum from 9.2 to 12.2psi @22:57:49.45 SS.reconfigure :inhaling 0.93ml/s @22:57:49.49 SS.seek 25ml @22:57:55.88 Vacuum too high -- waiting up to 40 seconds @22:57:55.90 SS.reconfigure :exhale @22:57:55.94 SS.hold @22:57:57.58 Intake @14.6psia, vacuum from 9.1 to 12.1psi @22:57:57.60 SS.reconfigure :inhaling 0.84ml/s @22:57:57.64 SS.seek 25ml @22:58:07.95 Vacuum too high -- waiting up to 40 seconds @22:58:07.96 SS.reconfigure :exhale @22:58:08.00 SS.hold @22:58:09.64 Intake @14.3psia, vacuum from 8.8 to 11.8psi @22:58:09.66 SS.reconfigure :inhaling 0.76ml/s @22:58:09.70 SS.seek 25ml @22:58:15.09 Stroke completed -- waiting for pressure to equalize ... @22:58:15.10 SS.reconfigure :exhale @22:58:15.15 SS.hold @22:58:17.63 Intake.select :close @22:58:17.77 SSV.dial :exhaust @22:58:18.50 Exhaust.select :open @22:58:18.61 SS.seek 0ml @22:58:27.78 Exhaust.select :close @22:58:27.89 SSV.dial CBV @22:58:27.91 CBV.dial SSV @22:58:28.64 Allowing 130s for 25.0ml of remaining 950.0ml @22:58:28.66 Intake.select :open @22:58:30.81 Intake @14.8psia, vacuum from 9.3 to 12.3psi @22:58:31.31 SS.reconfigure :inhaling 0.76ml/s @22:58:31.36 SS.seek 25ml @22:58:46.45 Vacuum too high -- waiting up to 40 seconds @22:58:46.46 SS.reconfigure :exhale @22:58:46.50 SS.hold @22:58:47.79 Intake @14.3psia, vacuum from 8.8 to 11.8psi @22:58:47.81 SS.reconfigure :inhaling 0.69ml/s @22:58:47.85 SS.seek 25ml @22:59:10.34 Stroke completed -- waiting for pressure to equalize ... @22:59:10.35 SS.reconfigure :exhale @22:59:10.40 SS.hold @22:59:14.76 Intake.select :close @22:59:14.90 SSV.dial :exhaust @22:59:15.63 Exhaust.select :open @22:59:15.74 SS.seek 0ml @22:59:25.12 Exhaust.select :close @22:59:25.23 SSV.dial CBV @22:59:25.27 CBV.dial SSV @22:59:26.00 Allowing 130s for 25.0ml of remaining 925.0ml @22:59:26.01 Intake.select :open @22:59:28.17 Intake @14.7psia, vacuum from 9.2 to 12.2psi @22:59:28.18 SS.reconfigure :inhaling 0.69ml/s @22:59:28.23 SS.seek 25ml @22:59:53.76 Vacuum too high -- waiting up to 40 seconds @22:59:53.77 SS.reconfigure :exhale @22:59:53.81 SS.hold @22:59:55.10 Intake @13.7psia, vacuum from 8.2 to 11.2psi @22:59:55.12 SS.reconfigure :inhaling 0.61ml/s @22:59:55.17 SS.seek 25ml @22:59:57.03 recoverPRVreagent08 @22:59:57.04 PTV.dial :mixing @22:59:57.75 PBV.dial :waste1 @22:59:58.12 delay 30 seconds @23:00:09.43 Stroke completed -- waiting for pressure to equalize ... @23:00:09.45 SS.reconfigure :exhale @23:00:09.49 SS.hold @23:00:13.03 Intake.select :close @23:00:13.17 SSV.dial :exhaust @23:00:13.89 Exhaust.select :open @23:00:14.00 SS.seek 0ml @23:00:22.95 Exhaust.select :close @23:00:23.06 SSV.dial CBV @23:00:23.08 CBV.dial SSV @23:00:23.81 Allowing 130s for 25.0ml of remaining 900.0ml @23:00:23.82 Intake.select :open @23:00:25.98 Intake @14.7psia, vacuum from 9.2 to 12.2psi @23:00:25.99 SS.reconfigure :inhaling 0.61ml/s @23:00:26.04 SS.seek 25ml @23:00:28.13 PBV.dial between PSV and waste1 @23:00:28.49 PSV.dial :air @23:00:28.95 PS.reconfigure :fast @23:00:28.99 PS.seek 2.5ml @23:00:51.41 PSV.dial :mixloop @23:00:51.85 delay 2 seconds @23:00:53.86 PSV.dial :air @23:00:54.32 delay 2 seconds @23:00:56.33 PSV.dial :mixloop @23:00:56.79 delay 2 seconds @23:00:58.80 PSV.dial :air @23:00:59.26 PS.seek 0ml @23:01:09.32 Stroke completed -- waiting for pressure to equalize ... @23:01:09.33 SS.reconfigure :exhale @23:01:09.38 SS.hold @23:01:14.21 Intake.select :close @23:01:14.37 SSV.dial :exhaust @23:01:15.57 Exhaust.select :open @23:01:15.69 SS.seek 0ml @23:01:21.69 washCycleDA #1 of 2 @23:01:21.70 PSR.select :air @23:01:21.72 PSV.dial PSR @23:01:23.06 Pulling 0.5ml of PSR.wash @23:01:23.07 PSR.select :wash @23:01:23.18 PS.reconfigure :default @23:01:23.22 PS.seek 0.5ml @23:01:24.68 Exhaust.select :close @23:01:24.79 SSV.dial CBV @23:01:24.82 CBV.dial SSV @23:01:25.58 Allowing 130s for 25.0ml of remaining 875.0ml @23:01:25.59 Intake.select :open @23:01:27.74 Intake @14.7psia, vacuum from 9.2 to 12.2psi @23:01:27.76 SS.reconfigure :inhaling 0.61ml/s @23:01:27.80 SS.seek 25ml @23:01:29.16 delay 5 seconds @23:01:34.18 PSR.select :air @23:01:34.29 PS.seek 2ml @23:01:50.31 PTV.dial :puck @23:01:51.03 PSV.dial PTV @23:01:51.74 PBV.dial :waste1 @23:01:52.15 PS.seek 1.5ml @23:01:58.09 delay 3 seconds @23:02:01.11 PBV.dial between PSV and waste1 @23:02:01.48 PS.seek 0ml @23:02:04.53 Vacuum too high -- waiting up to 40 seconds @23:02:04.54 SS.reconfigure :exhale @23:02:04.59 SS.hold @23:02:07.09 Intake @13.9psia, vacuum from 8.4 to 11.4psi @23:02:07.11 SS.reconfigure :inhaling 0.56ml/s @23:02:07.15 SS.seek 25ml @23:02:14.82 Stroke completed -- waiting for pressure to equalize ... @23:02:14.83 SS.reconfigure :exhale @23:02:14.87 SS.hold @23:02:17.50 delay 2:00 @23:02:18.42 Intake.select :close @23:02:18.56 SSV.dial :exhaust @23:02:19.29 Exhaust.select :open @23:02:19.40 SS.seek 0ml @23:02:28.71 Exhaust.select :close @23:02:28.82 SSV.dial CBV @23:02:28.85 CBV.dial SSV @23:02:29.57 Allowing 130s for 25.0ml of remaining 850.0ml @23:02:29.59 Intake.select :open @23:02:31.74 Intake @14.7psia, vacuum from 9.2 to 12.2psi @23:02:31.76 SS.reconfigure :inhaling 0.56ml/s @23:02:31.80 SS.seek 25ml @23:03:05.09 Vacuum too high -- waiting up to 40 seconds @23:03:05.10 SS.reconfigure :exhale @23:03:05.15 SS.hold @23:03:06.79 Intake @13.9psia, vacuum from 8.4 to 11.4psi @23:03:06.81 SS.reconfigure :inhaling 0.50ml/s @23:03:06.85 SS.seek 25ml @23:03:22.55 Stroke completed -- waiting for pressure to equalize ... @23:03:22.56 SS.reconfigure :exhale @23:03:22.61 SS.hold @23:03:26.76 Intake.select :close @23:03:26.90 SSV.dial :exhaust @23:03:27.64 Exhaust.select :open @23:03:27.75 SS.seek 0ml @23:03:36.72 Exhaust.select :close @23:03:36.83 SSV.dial CBV @23:03:36.86 CBV.dial SSV @23:03:37.62 Allowing 130s for 25.0ml of remaining 825.0ml @23:03:37.63 Intake.select :open @23:03:39.78 Intake @14.8psia, vacuum from 9.3 to 12.3psi @23:03:39.80 SS.reconfigure :inhaling 0.50ml/s @23:03:39.84 SS.seek 25ml @23:04:17.52 recoverPSRreagent08 @23:04:17.53 PTV.dial :puck @23:04:17.54 PBV.dial :waste1 @23:04:17.89 delay 30 seconds @23:04:33.97 Stroke completed -- waiting for pressure to equalize ... @23:04:33.98 SS.reconfigure :exhale @23:04:34.03 SS.hold @23:04:39.55 Intake.select :close @23:04:39.69 SSV.dial :exhaust @23:04:40.43 Exhaust.select :open @23:04:40.54 SS.seek 0ml @23:04:48.39 PBV.dial between PSV and waste1 @23:04:48.75 PSR.select :air @23:04:48.77 PSV.dial PSR @23:04:49.49 PS.reconfigure :fast @23:04:49.53 PS.seek 2.5ml @23:04:49.91 Exhaust.select :close @23:04:50.02 SSV.dial CBV @23:04:50.04 CBV.dial SSV @23:04:50.77 Allowing 130s for 25.0ml of remaining 800.0ml @23:04:50.78 Intake.select :open @23:04:52.94 Intake @14.8psia, vacuum from 9.3 to 12.3psi @23:04:52.95 SS.reconfigure :inhaling 0.50ml/s @23:04:53.00 SS.seek 25ml @23:05:12.18 PSV.dial PTV @23:05:13.01 delay 5 seconds @23:05:18.03 PSV.dial PSR @23:05:18.73 delay 5 seconds @23:05:23.76 PSV.dial PTV @23:05:24.47 delay 5 seconds @23:05:29.49 PSV.dial :air @23:05:30.20 PS.seek 0ml @23:05:46.88 Stroke completed -- waiting for pressure to equalize ... @23:05:46.89 SS.reconfigure :exhale @23:05:46.93 SS.hold @23:05:52.81 washCycleDA #2 of 2 @23:05:52.82 PSR.select :air @23:05:52.83 PSV.dial PSR @23:05:54.12 Pulling 0.5ml of PSR.wash @23:05:54.13 PSR.select :wash @23:05:54.33 PS.reconfigure :default @23:05:54.38 Intake.select :close @23:05:54.41 PS.seek 0.5ml @23:05:54.56 SSV.dial :exhaust @23:05:55.30 Exhaust.select :open @23:05:55.41 SS.seek 0ml @23:06:00.31 delay 5 seconds @23:06:04.47 Exhaust.select :close @23:06:04.58 SSV.dial CBV @23:06:04.60 CBV.dial SSV @23:06:05.33 PSR.select :air @23:06:05.39 Allowing 130s for 25.0ml of remaining 775.0ml @23:06:05.42 Intake.select :open @23:06:05.48 PS.seek 2ml @23:06:07.58 Intake @14.8psia, vacuum from 9.3 to 12.3psi @23:06:07.60 SS.reconfigure :inhaling 0.50ml/s @23:06:07.64 SS.seek 25ml @23:06:21.55 PTV.dial :puck @23:06:21.57 PSV.dial PTV @23:06:22.38 PBV.dial :waste1 @23:06:22.80 PS.seek 1.5ml @23:06:28.77 delay 3 seconds @23:06:31.79 PBV.dial between PSV and waste1 @23:06:32.16 PS.seek 0ml @23:06:48.21 delay 2:00 @23:06:52.15 Vacuum too high -- waiting up to 40 seconds @23:06:52.16 SS.reconfigure :exhale @23:06:52.20 SS.hold @23:06:57.06 Intake @14.9psia, vacuum from 9.4 to 12.4psi @23:06:57.08 SS.reconfigure :inhaling 0.46ml/s @23:06:57.12 SS.seek 25ml @23:07:08.80 Stroke completed -- waiting for pressure to equalize ... @23:07:08.81 SS.reconfigure :exhale @23:07:08.85 SS.hold @23:07:13.63 Intake.select :close @23:07:13.77 SSV.dial :exhaust @23:07:14.52 Exhaust.select :open @23:07:14.63 SS.seek 0ml @23:07:23.65 Exhaust.select :close @23:07:23.76 SSV.dial CBV @23:07:23.79 CBV.dial SSV @23:07:24.53 Allowing 130s for 25.0ml of remaining 750.0ml @23:07:24.54 Intake.select :open @23:07:26.70 Intake @14.8psia, vacuum from 9.3 to 12.3psi @23:07:26.72 SS.reconfigure :inhaling 0.46ml/s @23:07:26.76 SS.seek 25ml @23:08:29.20 Stroke completed -- waiting for pressure to equalize ... @23:08:29.21 SS.reconfigure :exhale @23:08:29.25 SS.hold @23:08:37.80 Intake.select :close @23:08:37.94 SSV.dial :exhaust @23:08:38.67 Exhaust.select :open @23:08:38.78 SS.seek 0ml @23:08:48.21 Exhaust.select :close @23:08:48.27 recoverPSRreagent08 @23:08:48.28 PTV.dial :puck @23:08:48.29 PBV.dial :waste1 @23:08:48.33 SSV.dial CBV @23:08:48.38 CBV.dial SSV @23:08:48.63 delay 30 seconds @23:08:49.09 Allowing 130s for 25.0ml of remaining 725.0ml @23:08:49.10 Intake.select :open @23:08:51.26 Intake @14.9psia, vacuum from 9.4 to 12.4psi @23:08:51.27 SS.reconfigure :inhaling 0.46ml/s @23:08:51.32 SS.seek 25ml @23:09:18.65 PBV.dial between PSV and waste1 @23:09:19.01 PSR.select :air @23:09:19.02 PSV.dial PSR @23:09:19.73 PS.reconfigure :fast @23:09:19.77 PS.seek 2.5ml @23:09:42.24 Vacuum too high -- waiting up to 40 seconds @23:09:42.25 SS.reconfigure :exhale @23:09:42.29 SS.hold @23:09:42.40 PSV.dial PTV @23:09:43.13 delay 5 seconds @23:09:46.51 Intake @15.0psia, vacuum from 9.5 to 12.5psi @23:09:46.53 SS.reconfigure :inhaling 0.41ml/s @23:09:46.57 SS.seek 25ml @23:09:48.15 PSV.dial PSR @23:09:48.86 delay 5 seconds @23:09:53.88 PSV.dial PTV @23:09:54.60 delay 5 seconds @23:09:59.62 PSV.dial :air @23:10:00.12 Stroke completed -- waiting for pressure to equalize ... @23:10:00.13 SS.reconfigure :exhale @23:10:00.17 SS.hold @23:10:00.34 PS.seek 0ml @23:10:06.63 Intake.select :close @23:10:07.28 SSV.dial :exhaust @23:10:08.01 Exhaust.select :open @23:10:08.12 SS.seek 0ml @23:10:17.45 Exhaust.select :close @23:10:17.55 SSV.dial CBV @23:10:17.58 CBV.dial SSV @23:10:18.32 Allowing 130s for 25.0ml of remaining 700.0ml @23:10:18.34 Intake.select :open @23:10:20.49 Intake @14.9psia, vacuum from 9.4 to 12.4psi @23:10:20.51 SS.reconfigure :inhaling 0.41ml/s @23:10:20.55 SS.seek 25ml @23:10:23.05 getPMLReagent08 @23:10:23.06 PRV.dial :air @23:10:23.50 delay 5 seconds @23:10:28.52 PTV.dial :PRVmixing @23:10:29.75 delay 5 seconds @23:10:34.77 PRV.dial :ab2 @23:10:35.21 PTV.dial :PRVmixing @23:10:35.22 PSV.dial :mixloop @23:10:35.66 PS.reconfigure :default @23:10:35.71 PS.seek 0.3ml @23:10:39.64 delay 2 seconds @23:10:41.66 PRV.dial :air @23:10:42.17 PS.seek 0.55ml @23:10:45.55 addPMLReagent08 @23:10:45.56 PSV.dial :air @23:10:46.01 PS.seek 2ml @23:11:01.70 PSV.dial :PML @23:11:02.14 PTV.dial :mixing @23:11:02.86 PBV.dial :waste1 @23:11:03.26 PS.seek 1.85ml @23:11:05.70 PBV.dial between PSV and waste1 @23:11:06.07 PS.seek 0ml @23:11:25.67 delay 2 seconds @23:11:27.69 delay 8:00 @23:11:31.44 Stroke completed -- waiting for pressure to equalize ... @23:11:31.45 SS.reconfigure :exhale @23:11:31.50 SS.hold @23:11:41.15 Intake.select :close @23:11:41.29 SSV.dial :exhaust @23:11:42.03 Exhaust.select :open @23:11:42.14 SS.seek 0ml @23:11:51.46 Exhaust.select :close @23:11:51.56 SSV.dial CBV @23:11:51.59 CBV.dial SSV @23:11:52.35 Allowing 130s for 25.0ml of remaining 675.0ml @23:11:52.36 Intake.select :open @23:11:54.52 Intake @14.9psia, vacuum from 9.4 to 12.4psi @23:11:54.53 SS.reconfigure :inhaling 0.41ml/s @23:11:54.58 SS.seek 25ml @23:13:00.36 Vacuum too high -- waiting up to 40 seconds @23:13:00.37 SS.reconfigure :exhale @23:13:00.41 SS.hold @23:13:05.28 Intake @14.4psia, vacuum from 8.9 to 11.9psi @23:13:05.30 SS.reconfigure :inhaling 0.37ml/s @23:13:05.34 SS.seek 25ml @23:13:12.70 Stroke completed -- waiting for pressure to equalize ... @23:13:12.72 SS.reconfigure :exhale @23:13:12.76 SS.hold @23:13:18.38 Intake.select :close @23:13:18.52 SSV.dial :exhaust @23:13:19.25 Exhaust.select :open @23:13:19.36 SS.seek 0ml @23:13:28.61 Exhaust.select :close @23:13:29.21 SSV.dial CBV @23:13:29.24 CBV.dial SSV @23:13:29.97 Allowing 130s for 25.0ml of remaining 650.0ml @23:13:29.99 Intake.select :open @23:13:32.14 Intake @14.9psia, vacuum from 9.4 to 12.4psi @23:13:32.16 SS.reconfigure :inhaling 0.37ml/s @23:13:32.21 SS.seek 25ml @23:14:53.69 Stroke completed -- waiting for pressure to equalize ... @23:14:53.70 SS.reconfigure :exhale @23:14:53.74 SS.hold @23:15:04.77 Intake.select :close @23:15:04.92 SSV.dial :exhaust @23:15:05.66 Exhaust.select :open @23:15:05.77 SS.seek 0ml @23:15:15.15 Exhaust.select :close @23:15:15.26 SSV.dial CBV @23:15:15.29 CBV.dial SSV @23:15:16.05 Allowing 130s for 25.0ml of remaining 625.0ml @23:15:16.06 Intake.select :open @23:15:18.21 Intake @15.0psia, vacuum from 9.5 to 12.5psi @23:15:18.23 SS.reconfigure :inhaling 0.37ml/s @23:15:18.27 SS.seek 25ml @23:16:17.79 Vacuum too high -- waiting up to 40 seconds @23:16:17.80 SS.reconfigure :exhale @23:16:17.84 SS.hold @23:16:24.40 Intake @14.6psia, vacuum from 9.1 to 12.1psi @23:16:24.42 SS.reconfigure :inhaling 0.33ml/s @23:16:24.46 SS.seek 25ml @23:16:48.43 Stroke completed -- waiting for pressure to equalize ... @23:16:48.44 SS.reconfigure :exhale @23:16:48.49 SS.hold @23:16:58.25 Intake.select :close @23:16:58.39 SSV.dial :exhaust @23:16:59.12 Exhaust.select :open @23:16:59.23 SS.seek 0ml @23:17:08.26 Exhaust.select :close @23:17:08.37 SSV.dial CBV @23:17:08.40 CBV.dial SSV @23:17:09.12 Allowing 130s for 25.0ml of remaining 600.0ml @23:17:09.13 Intake.select :open @23:17:11.29 Intake @15.0psia, vacuum from 9.5 to 12.5psi @23:17:11.30 SS.reconfigure :inhaling 0.33ml/s @23:17:11.35 SS.seek 25ml @23:18:42.85 Stroke completed -- waiting for pressure to equalize ... @23:18:42.86 SS.reconfigure :exhale @23:18:42.91 SS.hold @23:18:57.21 Intake.select :close @23:18:57.35 SSV.dial :exhaust @23:18:58.09 Exhaust.select :open @23:18:58.20 SS.seek 0ml @23:19:07.26 Exhaust.select :close @23:19:07.36 SSV.dial CBV @23:19:07.39 CBV.dial SSV @23:19:08.15 Allowing 130s for 25.0ml of remaining 575.0ml @23:19:08.16 Intake.select :open @23:19:10.31 Intake @15.1psia, vacuum from 9.6 to 12.6psi @23:19:10.33 SS.reconfigure :inhaling 0.33ml/s @23:19:10.38 SS.seek 25ml @23:19:27.71 recoverPRVreagent08 @23:19:27.72 PTV.dial :mixing @23:19:27.73 PBV.dial :waste1 @23:19:28.08 delay 30 seconds @23:19:58.11 PBV.dial between PSV and waste1 @23:19:58.46 PSV.dial :air @23:19:58.91 PS.reconfigure :fast @23:19:58.96 PS.seek 2.5ml @23:20:21.69 PSV.dial :mixloop @23:20:22.14 delay 2 seconds @23:20:24.16 PSV.dial :air @23:20:24.61 delay 2 seconds @23:20:26.63 PSV.dial :mixloop @23:20:27.08 delay 2 seconds @23:20:27.31 Vacuum too high -- waiting up to 40 seconds @23:20:27.32 SS.reconfigure :exhale @23:20:27.36 SS.hold @23:20:29.09 PSV.dial :air @23:20:29.55 PS.seek 0ml @23:20:37.13 Intake @15.3psia, vacuum from 9.8 to 12.8psi @23:20:37.15 SS.reconfigure :inhaling 0.30ml/s @23:20:37.19 SS.seek 25ml @23:20:52.07 washCycleDA #1 of 3 @23:20:52.08 PSR.select :air @23:20:52.09 PSV.dial PSR @23:20:52.80 Stroke completed -- waiting for pressure to equalize ... @23:20:52.81 SS.reconfigure :exhale @23:20:52.85 SS.hold @23:20:53.54 Pulling 0.5ml of PSR.wash @23:20:53.55 PSR.select :wash @23:20:53.66 PS.reconfigure :default @23:20:53.75 PS.seek 0.5ml @23:20:59.85 delay 5 seconds @23:21:04.03 Intake.select :close @23:21:04.18 SSV.dial :exhaust @23:21:04.86 PSR.select :air @23:21:04.92 Exhaust.select :open @23:21:04.98 PS.seek 2ml @23:21:05.03 SS.seek 0ml @23:21:14.08 Exhaust.select :close @23:21:14.19 SSV.dial CBV @23:21:14.21 CBV.dial SSV @23:21:14.94 Allowing 130s for 25.0ml of remaining 550.0ml @23:21:14.95 Intake.select :open @23:21:17.11 Intake @15.0psia, vacuum from 9.5 to 12.5psi @23:21:17.12 SS.reconfigure :inhaling 0.30ml/s @23:21:17.17 SS.seek 25ml @23:21:21.03 PTV.dial :puck @23:21:21.73 PSV.dial PTV @23:21:22.45 PBV.dial :waste1 @23:21:22.87 PS.seek 1.5ml @23:21:28.78 delay 3 seconds @23:21:31.80 PBV.dial between PSV and waste1 @23:21:32.16 PS.seek 0ml @23:21:48.75 delay 2:00 @23:22:37.12 Vacuum too high -- waiting up to 40 seconds @23:22:37.13 SS.reconfigure :exhale @23:22:37.17 SS.hold @23:22:44.67 Intake @14.7psia, vacuum from 9.2 to 12.2psi @23:22:44.69 SS.reconfigure :inhaling 0.27ml/s @23:22:44.73 SS.seek 25ml @23:23:06.81 Stroke completed -- waiting for pressure to equalize ... @23:23:06.82 SS.reconfigure :exhale @23:23:06.86 SS.hold @23:23:19.40 Intake.select :close @23:23:19.55 SSV.dial :exhaust @23:23:20.28 Exhaust.select :open @23:23:20.39 SS.seek 0ml @23:23:29.42 Exhaust.select :close @23:23:29.53 SSV.dial CBV @23:23:29.55 CBV.dial SSV @23:23:30.30 Allowing 130s for 25.0ml of remaining 525.0ml @23:23:30.31 Intake.select :open @23:23:32.46 Intake @15.1psia, vacuum from 9.6 to 12.6psi @23:23:32.48 SS.reconfigure :inhaling 0.27ml/s @23:23:32.52 SS.seek 25ml @23:23:48.77 recoverPSRreagent08 @23:23:48.78 PTV.dial :puck @23:23:48.79 PBV.dial :waste1 @23:23:49.14 delay 30 seconds @23:24:19.16 PBV.dial between PSV and waste1 @23:24:19.51 PSR.select :air @23:24:19.53 PSV.dial PSR @23:24:20.25 PS.reconfigure :fast @23:24:20.29 PS.seek 2.5ml @23:24:42.84 PSV.dial PTV @23:24:43.56 delay 5 seconds @23:24:48.58 PSV.dial PSR @23:24:49.29 delay 5 seconds @23:24:54.31 PSV.dial PTV @23:24:55.03 delay 5 seconds @23:25:00.04 PSV.dial :air @23:25:00.75 PS.seek 0ml @23:25:22.70 Stroke completed -- waiting for pressure to equalize ... @23:25:22.71 SS.reconfigure :exhale @23:25:22.76 SS.hold @23:25:23.18 washCycleDA #2 of 3 @23:25:23.19 PSR.select :air @23:25:23.21 PSV.dial PSR @23:25:24.50 Pulling 0.5ml of PSR.wash @23:25:24.51 PSR.select :wash @23:25:24.62 PS.reconfigure :default @23:25:24.71 PS.seek 0.5ml @23:25:30.68 delay 5 seconds @23:25:35.69 PSR.select :air @23:25:35.80 PS.seek 2ml @23:25:40.32 SS.reconfigure :default @23:25:40.36 SS.hold @23:25:40.47 Intake.select :close @23:25:40.62 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 500.0ml @23:25:40.63 SSV.dial :exhaust @23:25:41.87 Exhaust.select :open @23:25:41.98 SS.reconfigure :exhale @23:25:42.02 SS.seek 0ml @23:25:51.37 Exhaust.select :close @23:25:51.47 Sampled 500.0ml @23:25:51.50 Drying puck before fixing for 20:00 @23:25:51.51 CBV.dial :waste2b @23:25:51.83 PTV.dial :puck @23:25:51.85 PSV.dial PTV @23:25:52.23 delay 1:00 @23:25:52.54 PBV.dial :waste1 @23:25:52.96 PS.seek 1.5ml @23:25:58.90 delay 3 seconds @23:26:01.92 PBV.dial between PSV and waste1 @23:26:02.28 PS.seek 0ml @23:26:18.37 delay 2:00 @23:26:52.25 CSV.dial :air @23:26:52.79 CS.reconfigure :fast @23:26:52.84 CS.seek 10ml @23:27:15.29 Pumping wcr puck from tube 2 up to 35psi... @23:27:15.30 CBV.dial SSV @23:27:16.59 CBV.dial between CSV and waste2b @23:27:17.50 CSV.dial between air and CTV @23:27:17.81 CS.seek 7.535ml @23:27:24.10 CTV.dial CSV,avoiding: :air @23:27:24.13 CSV.dial CTV @23:27:24.92 CS.reconfigure :pressurize @23:27:24.96 CS.seek 0ml @23:27:40.12 CBV.dial :waste2b @23:27:40.45 CS.reconfigure :default @23:27:40.49 CS.seek 0ml @23:27:45.51 delay 1:00 @23:28:18.39 recoverPSRreagent08 @23:28:18.41 PTV.dial :puck @23:28:18.42 PBV.dial :waste1 @23:28:18.77 delay 30 seconds @23:28:45.54 CTV.dial :air,avoiding: :intake @23:28:46.79 Fix #1 of 2 (with RNAlater) for 10:00 @23:28:46.80 CSV.dial CSR @23:28:47.31 Pulling 2ml of CSR.RNAlater @23:28:47.32 CSR.select :RNAlater @23:28:47.43 CS.seek 2ml @23:28:48.79 PBV.dial between PSV and waste1 @23:28:49.14 PSR.select :air @23:28:49.16 PSV.dial PSR @23:28:49.88 PS.reconfigure :fast @23:28:49.93 PS.seek 2.5ml @23:28:53.35 delay 5 seconds @23:28:58.37 CSR.select :air @23:28:58.48 CS.reconfigure :fast @23:28:58.53 CS.seek 10ml @23:29:12.45 PSV.dial PTV @23:29:13.18 delay 5 seconds @23:29:16.72 Pumping wcr puck from tube 2 up to 35psi... @23:29:16.74 CBV.dial SSV @23:29:18.00 CBV.dial between CSV and waste2b @23:29:18.19 PSV.dial PSR @23:29:18.93 delay 5 seconds @23:29:18.95 CSV.dial between air and CTV @23:29:19.54 CS.seek 7.281ml @23:29:23.96 PSV.dial PTV @23:29:24.67 delay 5 seconds @23:29:26.26 CTV.dial CSV,avoiding: :air @23:29:26.29 CSV.dial CTV @23:29:27.57 CS.reconfigure :pressurize @23:29:27.62 CS.seek 0ml @23:29:29.69 PSV.dial :air @23:29:30.40 PS.seek 0ml @23:29:44.31 delay 10:00 @23:29:52.83 washCycleDA #3 of 3 @23:29:52.84 PSR.select :air @23:29:52.86 PSV.dial PSR @23:29:54.15 Pulling 0.5ml of PSR.wash @23:29:54.16 PSR.select :wash @23:29:54.27 PS.reconfigure :default @23:29:54.31 PS.seek 0.5ml @23:30:00.24 delay 5 seconds @23:30:05.26 PSR.select :air @23:30:05.37 PS.seek 2ml @23:30:21.36 PTV.dial :puck @23:30:21.37 PSV.dial PTV @23:30:22.06 PBV.dial :waste1 @23:30:22.96 PS.seek 1.5ml @23:30:28.86 delay 3 seconds @23:30:31.88 PBV.dial between PSV and waste1 @23:30:32.24 PS.seek 0ml @23:30:48.20 delay 2:00 @23:32:48.23 recoverPSRreagent08 @23:32:48.24 PTV.dial :puck @23:32:48.25 PBV.dial :waste1 @23:32:48.61 delay 30 seconds @23:33:18.63 PBV.dial between PSV and waste1 @23:33:18.98 PSR.select :air @23:33:19.00 PSV.dial PSR @23:33:19.72 PS.reconfigure :fast @23:33:19.76 PS.seek 2.5ml @23:33:42.29 PSV.dial PTV @23:33:43.01 delay 5 seconds @23:33:48.03 PSV.dial PSR @23:33:48.75 delay 5 seconds @23:33:53.76 PSV.dial PTV @23:33:54.59 delay 5 seconds @23:33:59.61 PSV.dial :air @23:34:00.34 PS.seek 0ml @23:34:22.73 Gate.power :camera,:ON @23:34:22.74 Mixing DA Substrates @23:34:22.75 PSR.select :air @23:34:22.76 PSV.dial PSR @23:34:24.31 Pulling 0.25ml of PSR.sub1 @23:34:24.35 PSR.select :sub1 @23:34:24.60 PS.reconfigure :default @23:34:24.76 PS.seek 0.25ml @23:34:28.23 delay 5 seconds @23:34:33.30 Pulling 0.25ml of PSR.sub2 @23:34:33.31 PSR.select :sub2 @23:34:33.42 PS.seek 0.5ml @23:34:36.76 delay 5 seconds @23:34:41.78 PSR.select :air @23:34:41.88 PS.seek 2ml @23:34:57.94 PTV.dial :puck @23:34:57.96 PSV.dial PTV @23:34:58.66 PBV.dial :waste1 @23:34:59.10 PS.seek 1.5ml @23:35:05.01 delay 3 seconds @23:35:08.03 PBV.dial between PSV and waste1 @23:35:08.39 PS.seek 0ml @23:35:24.44 delay 10 seconds @23:35:34.47 recoverPSRreagent08 @23:35:34.48 PTV.dial :puck @23:35:34.49 PBV.dial :waste1 @23:35:34.84 delay 30 seconds @23:36:04.86 PBV.dial between PSV and waste1 @23:36:05.21 PSR.select :air @23:36:05.23 PSV.dial PSR @23:36:05.95 PS.reconfigure :fast @23:36:05.99 PS.seek 2.5ml @23:36:28.63 PSV.dial PTV @23:36:29.35 delay 5 seconds @23:36:34.37 PSV.dial PSR @23:36:35.08 delay 5 seconds @23:36:40.10 PSV.dial PTV @23:36:40.82 delay 5 seconds @23:36:45.84 PSV.dial :air @23:36:46.56 PS.seek 0ml @23:37:09.13 Additional air clear @23:37:09.14 PSR.select :air @23:37:09.15 PSV.dial PSR @23:37:10.57 Pulling 2ml of PSR.air @23:37:10.58 PSR.select :air @23:37:10.60 PS.reconfigure :default @23:37:10.64 PS.seek 2ml @23:37:31.72 delay 5 seconds @23:37:36.75 PTV.dial :puck @23:37:36.76 PSV.dial PTV @23:37:37.49 PBV.dial :waste1 @23:37:37.91 PS.seek 1.5ml @23:37:43.85 delay 3 seconds @23:37:46.86 PBV.dial between PSV and waste1 @23:37:47.23 delay 1 second @23:37:48.24 PSV.dial :waste1 @23:37:49.25 PS.seek 0ml @23:38:05.33 Processing Puck Heater at 28.1C before imaging @23:38:05.34 PH.coast @23:38:05.46 Imaging da puck in Processing Clamp while storing it to tube 1 @23:38:05.62 SC.reconfigure :tight @23:38:05.66 SC.seek 1 @23:38:09.24 SC.configure :limp @23:38:09.43 Elbow.reconfigure :tight @23:38:09.47 Elbow.seek 1 @23:38:11.15 Forearm.reconfigure :tight @23:38:11.19 Forearm.seek 1 + 700 counts @23:38:12.24 SE.reconfigure :pick @23:38:12.29 SE.seek :beamBreak @23:38:13.71 SE.configure :default @23:38:13.85 Elbow.reconfigure :limp @23:38:13.90 Forearm.reconfigure :loose @23:38:13.94 Forearm.seek 1 @23:38:14.33 Elbow.hold @23:38:14.39 Elbow.reconfigure :tight @23:38:14.48 SE.seek 25.37 Pucks below Top Plate @23:38:14.99 Forearm.force -20 @23:38:15.02 Forearm.seek 1 + 314 counts @23:38:15.42 Forearm.reconfigure :tight @23:38:15.97 Forearm.seek 1 + 700 counts @23:38:16.39 SE.seek 26.37 Pucks below Top Plate @23:38:17.04 Forearm.reconfigure :loose @23:38:17.08 Forearm.seek :retract @23:38:18.03 Elbow.seek PC @23:38:20.50 Elbow.reconfigure :loose @23:38:20.59 Forearm.seek PC @23:38:21.54 Hand.close @23:38:22.21 PC.seek :open @23:38:26.25 Forearm.seek :retract @23:38:27.32 Elbow.reconfigure :tight @23:38:27.37 Elbow.seek 1 @23:38:30.12 Forearm.reconfigure :tight @23:38:30.16 Forearm.seek 1 + 700 counts @23:38:31.15 Hand.open @23:38:31.89 SE.seek 26.01 Pucks below Top Plate @23:38:32.17 SE.reconfigure :seal @23:38:32.21 SE.seek 24.91 Pucks below Top Plate @23:38:32.77 Lights, camera, ACTION! @23:38:32.80 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22nov1023h975ml.tif @23:38:49.44 SX MX5/16 (USB:13): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 13.152 seconds /var/log/esp/da22nov1023h975ml.tif: TIFF Upload Complete @23:38:49.47 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22nov1023h975ml15s.tif @23:38:52.26 Waiting >20 seconds for da to relinquish FlushPuck @23:39:05.20 SX MX5/16 (USB:13): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22nov1023h975ml15s.tif: TIFF Upload Complete @23:39:05.23 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da22nov1023h975ml25s.tif @23:39:30.96 SX MX5/16 (USB:13): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da22nov1023h975ml25s.tif: TIFF Upload Complete @23:39:30.98 Gate.power :camera,:OFF @23:39:30.99 DAfans.email "13.152 sec auto-exposure",Subject: "da22nov1023h975ml images!" @23:39:31.30 Elbow.seek 1 @23:39:32.01 Forearm.seek 1 + 700 counts @23:39:32.08 SE.reconfigure :gently @23:39:32.13 SE.seek 25.62 Pucks below Top Plate @23:39:32.82 Elbow.reconfigure :limp @23:39:32.87 Forearm.reconfigure :loose @23:39:32.93 Forearm.seek 1 @23:39:33.32 Elbow.hold @23:39:33.39 Elbow.reconfigure :tight @23:39:33.44 SE.reconfigure :default @23:39:33.48 SE.seek 25.81 Pucks below Top Plate @23:39:34.00 Forearm.force -20 @23:39:34.04 Forearm.seek 1 + 320 counts @23:39:34.43 Forearm.reconfigure :tight @23:39:34.47 Forearm.seek 1 + 700 counts @23:39:34.89 SE.seek :down @23:39:35.90 SE.stop @23:39:36.23 Forearm.reconfigure :loose @23:39:36.34 Forearm.seek :retract @23:39:37.43 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @23:39:37.74 Elbow.seek FlushPuck::Garage @23:39:39.80 Elbow.reconfigure :loose @23:39:39.98 Forearm.seek :garage @23:39:41.15 Hand.close @23:39:42.12 Forearm.seek :retract @23:39:43.29 Elbow.reconfigure :tight @23:39:43.38 Elbow.seek PC @23:39:44.32 CBV.dial :waste2b @23:39:44.70 CS.reconfigure :default @23:39:44.93 Elbow.reconfigure :loose @23:39:44.99 CS.seek 0ml @23:39:45.31 Forearm.reconfigure :tight @23:39:45.39 Forearm.seek PC @23:39:48.29 delay 1:00 @23:39:48.36 PC.seek :closed @23:39:52.39 Processing Clamp onSpring/offSpring for 9/24 samples, on/off torque 1.42 @23:39:52.55 Hand.open @23:39:53.48 Forearm.reconfigure :loose @23:39:53.56 Forearm.seek :retract @23:39:54.69 PSR.select :air @23:39:54.74 PSV.dial PSR @23:39:55.40 Pulling 0.5ml of PSR.flush @23:39:55.42 PSR.select :flush @23:39:55.58 PS.seek 0.5ml @23:40:01.50 delay 5 seconds @23:40:06.53 PSR.select :air @23:40:06.69 PS.seek 2.5ml @23:40:27.90 delay 1 second @23:40:28.94 PRV.dial :waste2,avoiding: :ab2 @23:40:30.76 PTV.dial :PRVmixing @23:40:32.03 PSV.dial :mixloop @23:40:33.10 PS.seek :empty @23:40:48.35 CTV.dial :air,avoiding: :intake @23:40:49.65 Fix #2 of 2 (with RNAlater) for 10:00 @23:40:49.67 CSV.dial CSR @23:40:50.23 Pulling 2ml of CSR.RNAlater @23:40:50.25 CSR.select :RNAlater @23:40:50.39 CS.seek 2ml @23:40:56.30 delay 5 seconds @23:40:59.46 PSR.select :air @23:40:59.49 PSV.dial PSR @23:41:00.65 Pulling 0.5ml of PSR.flush @23:41:00.67 PSR.select :flush @23:41:00.85 PS.seek 0.5ml @23:41:01.34 CSR.select :air @23:41:01.50 CS.reconfigure :fast @23:41:01.60 CS.seek 10ml @23:41:06.80 delay 5 seconds @23:41:11.83 PSR.select :air @23:41:11.99 PS.seek 2.5ml @23:41:20.11 Pumping wcr puck from tube 2 up to 35psi... @23:41:20.13 CBV.dial SSV @23:41:21.49 CBV.dial between CSV and waste2b @23:41:22.56 CSV.dial between air and CTV @23:41:23.24 CS.seek 7.238ml @23:41:30.16 CTV.dial CSV,avoiding: :air @23:41:30.27 CSV.dial CTV @23:41:31.66 CS.reconfigure :pressurize @23:41:31.76 CS.seek 0ml @23:41:33.22 delay 1 second @23:41:34.23 PBV.dial :waste1 @23:41:34.64 PTV.dial :mixing @23:41:35.41 PSV.dial :mixloop @23:41:36.45 PS.seek :empty @23:41:48.48 delay 10:00 @23:42:02.35 upsync @23:42:02.75 PSV.dial PSR @23:42:03.73 PSR.select :flush @23:42:03.84 PS.seek 1ml @23:42:14.77 PSR.select :air @23:42:14.89 PS.seek 2.5ml @23:42:30.90 PSV.dial PTV @23:42:31.60 PTV.dial :puck @23:42:32.29 PBV.dial :waste1 @23:42:32.31 PS.seek 0ml @23:42:58.56 PSV.dial PSR @23:42:59.27 PSR.select :flush @23:42:59.39 PS.seek 0.5ml @23:43:05.24 PSR.select :air @23:43:05.35 PS.seek 2.5ml @23:43:26.49 PSV.dial PTV @23:43:27.21 PBV.dial PSV @23:43:27.75 PS.seek 0ml @23:43:54.08 PSV.dial PBV @23:43:54.54 PS.seek 2.5ml @23:44:20.71 PSV.dial :waste1 @23:44:21.42 PS.seek 0ml @23:44:47.61 PSV.dial PSR @23:44:48.52 PSR.select :air @23:44:48.54 PS.seek 2.5ml @23:45:14.68 PSV.dial PTV @23:45:15.38 PBV.dial PSV @23:45:15.40 PS.seek 0ml @23:45:41.61 PSV.dial PBV @23:45:42.07 PS.seek 2.5ml @23:46:08.33 PSV.dial :waste1 @23:46:09.04 PS.seek 0ml @23:46:35.24 PSV.dial PSR @23:46:35.67 PSR.select :flush @23:46:35.79 PS.seek 1ml @23:46:46.74 PSR.select :air @23:46:46.85 PS.seek 2.5ml @23:47:02.89 PSV.dial PTV @23:47:03.60 PTV.dial :puck @23:47:03.61 PBV.dial :waste1 @23:47:04.13 PS.seek 0ml @23:47:30.30 PSV.dial PSR @23:47:31.02 PSR.select :flush @23:47:31.13 PS.seek 0.5ml @23:47:37.08 PSR.select :air @23:47:37.19 PS.seek 2.5ml @23:47:58.30 PSV.dial PTV @23:47:59.02 PBV.dial PSV @23:47:59.57 PS.seek 0ml @23:48:25.76 PSV.dial PBV @23:48:26.22 PS.seek 2.5ml @23:48:52.45 PSV.dial :waste1 @23:48:53.16 PS.seek 0ml @23:49:19.36 PSV.dial PSR @23:49:19.79 PSR.select :air @23:49:19.82 PS.seek 2.5ml @23:49:46.04 PSV.dial PTV @23:49:46.75 PBV.dial PSV @23:49:46.77 PS.seek 0ml @23:50:12.92 PSV.dial PBV @23:50:13.38 PS.seek 2.5ml @23:50:39.64 PSV.dial :waste1 @23:50:40.35 PS.seek 0ml @23:51:06.70 Garaging FlushPuck in Processing Clamp @23:51:06.77 Elbow.reconfigure :tight @23:51:06.82 Elbow.seek PC @23:51:06.93 Elbow.reconfigure :loose @23:51:07.03 Forearm.seek PC @23:51:08.10 Hand.close @23:51:08.69 PC.seek :open @23:51:12.65 Forearm.seek :retract @23:51:13.69 Elbow.reconfigure :tight @23:51:13.73 Elbow.seek :garage @23:51:15.08 Elbow.reconfigure :loose @23:51:15.17 Forearm.reconfigure :tight @23:51:15.21 Forearm.seek :garage @23:51:16.34 Hand.open @23:51:17.12 Forearm.reconfigure :loose @23:51:17.16 Forearm.seek :retract @23:51:48.52 CBV.dial :waste2b @23:51:48.84 CS.reconfigure :default @23:51:48.89 CS.seek 0ml @23:51:51.62 delay 1:00 @23:52:51.65 CTV.dial :air,avoiding: :intake @23:52:52.94 Storing wcr puck in Collection Clamp to tube 1 @23:52:53.32 Elbow.reconfigure :tight @23:52:53.36 Elbow.seek 1 @23:52:55.21 Forearm.reconfigure :tight @23:52:55.26 Forearm.seek 1 + 700 counts @23:52:56.29 SE.reconfigure :pick @23:52:56.34 SE.seek :beamBreak @23:52:57.75 SE.configure :default @23:52:57.90 Elbow.reconfigure :limp @23:52:57.94 Forearm.reconfigure :loose @23:52:57.99 Forearm.seek 1 @23:52:58.38 Elbow.hold @23:52:58.45 Elbow.reconfigure :tight @23:52:59.01 SE.seek 26.38 Pucks below Top Plate @23:52:59.45 Forearm.force -20 @23:52:59.50 Forearm.seek 1 + 315 counts @23:52:59.88 Forearm.reconfigure :tight @23:52:59.92 Forearm.seek 1 + 700 counts @23:53:00.27 SE.seek 27.38 Pucks below Top Plate @23:53:00.79 Forearm.reconfigure :loose @23:53:00.84 Forearm.seek :retract @23:53:01.75 Elbow.seek CC @23:53:03.39 Elbow.reconfigure :loose @23:53:03.49 Forearm.seek CC @23:53:04.49 Hand.close @23:53:05.06 CC.seek :open @23:53:09.49 Forearm.seek :retract @23:53:10.48 Elbow.reconfigure :tight @23:53:10.53 Elbow.seek 1 @23:53:12.29 Elbow.reconfigure :limp @23:53:12.34 Forearm.seek 1 @23:53:13.46 Elbow.hold @23:53:13.53 Elbow.reconfigure :tight @23:53:13.57 Hand.open @23:53:14.42 Forearm.force -20 @23:53:14.46 Forearm.seek 1 + 317 counts @23:53:14.84 Forearm.reconfigure :tight @23:53:14.89 Forearm.seek 1 + 700 counts @23:53:15.28 SE.seek :down @23:53:16.09 SE.stop @23:53:16.40 Forearm.reconfigure :loose @23:53:16.44 Forearm.seek :retract @23:53:17.42 Starting finalPurge @23:53:17.43 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @23:53:17.61 Elbow.seek FlushPuck::Garage @23:53:19.36 Elbow.reconfigure :loose @23:53:19.45 Forearm.seek :garage @23:53:20.54 Hand.close @23:53:21.29 Forearm.seek :retract @23:53:22.34 Elbow.reconfigure :tight @23:53:22.38 Elbow.seek CC @23:53:25.30 Elbow.reconfigure :loose @23:53:25.38 Forearm.reconfigure :tight @23:53:25.42 Forearm.seek CC @23:53:26.68 CC.seek :closed @23:53:30.92 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.93 @23:53:30.99 Hand.open @23:53:31.74 Forearm.reconfigure :loose @23:53:31.78 Forearm.seek :retract @23:53:32.87 Flushing the Collection Valve::Manifold @23:53:32.98 CSV.dial CSR @23:53:33.45 Pulling 5ml of CSR.flush @23:53:33.46 CSR.select :flush @23:53:33.58 CS.seek 5ml @23:53:46.96 delay 5 seconds @23:53:51.98 CSR.select :air @23:53:52.13 CS.reconfigure :fast @23:53:52.17 CS.seek 7.5ml @23:53:58.46 CSV.dial :waste @23:53:59.70 CS.reconfigure :default @23:53:59.74 CS.seek 0ml @23:54:19.46 Flushing lower manifold's waste1 port @23:54:20.05 CSV.dial :flush @23:54:20.48 CS.reconfigure :fast @23:54:20.53 CS.seek 5ml @23:54:32.15 delay 5 seconds @23:54:37.17 CSV.dial :air @23:54:37.77 CS.seek 7ml @23:54:42.93 CSV.dial CTV @23:54:42.96
CTV.dial CSV @23:54:44.38 CBV.dial :waste1 @23:54:45.12 CS.seek 0ml @23:55:01.02 Flushing lower manifold's waste2 port @23:55:01.14 CSV.dial :flush @23:55:01.86 CS.seek 5ml @23:55:13.46 delay 5 seconds @23:55:18.48 CSV.dial :air @23:55:18.99 CS.seek 6.999ml @23:55:24.15 CSV.dial CTV @23:55:24.18
CTV.dial CSV @23:55:24.60 CBV.dial :waste2 @23:55:25.61 CS.seek 0ml @23:55:41.43 Flushing lower manifold's waste2b port @23:55:41.54 CSV.dial :flush @23:55:42.26 CS.seek 5ml @23:55:53.90 delay 5 seconds @23:55:58.91 CSV.dial :air @23:55:59.42 CS.seek 7ml @23:56:04.75 CSV.dial CTV @23:56:04.77
CTV.dial CSV @23:56:05.19 CBV.dial :waste2b @23:56:06.18 CS.seek 0ml @23:56:22.05 Disinfecting external Intake with kill @23:56:22.16 CSV.dial CSR @23:56:22.65 Pulling 8ml of CSR.kill @23:56:22.66 CSR.select :kill @23:56:22.77 CS.seek 8ml @23:56:40.96 delay 5 seconds @23:56:45.98 CSR.select :air @23:56:46.09 CS.seek 10ml @23:56:51.25 CSV.dial CBV @23:56:51.27
CBV.dial CSV @23:56:52.30 CTV.dial :intake @23:56:53.02 Intake.select :open @23:56:53.13 CS.seek 2ml @23:57:11.08 Intake.select :close @23:57:11.18 CTV.dial :air @23:57:11.91 CS.reconfigure :default @23:57:11.95 CS.seek 0ml @23:57:17.77 CSV.dial CSR @23:57:18.79 Pulling 5ml of CSR.flush @23:57:18.80 CSR.select :flush @23:57:18.91 CS.seek 5ml @23:57:32.27 delay 5 seconds @23:57:37.29 CSR.select :air @23:57:37.40 CS.seek 10ml @23:57:50.80 CSV.dial CBV @23:57:51.77 CS.seek 0ml @23:58:17.74 Garaging FlushPuck in Collection Clamp @23:58:17.80 Elbow.reconfigure :tight @23:58:17.85 Elbow.seek CC @23:58:17.96 Elbow.reconfigure :loose @23:58:18.05 Forearm.seek CC @23:58:19.03 Hand.close @23:58:19.67 CC.seek :open @23:58:24.00 Forearm.seek :retract @23:58:25.00 Elbow.reconfigure :tight @23:58:25.04 Elbow.seek :garage @23:58:28.07 Elbow.reconfigure :loose @23:58:28.16 Forearm.reconfigure :tight @23:58:28.20 Forearm.seek :garage @23:58:29.30 Hand.open @23:58:30.10 Forearm.reconfigure :loose @23:58:30.14 Forearm.seek :retract @23:58:31.19 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 21:15:22 @23:58:31.23 Asleep after completing DA_WCR_111022 assays @23:58:31.25 GoodNews.email "{1 => 28,\n 2 => 5,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@23:54:21, 24.1C, 28% humidity, 15.7psia, 14.031V, 0.355A, 0.355A avg, 4.98W\nSampled 500.0ml",Subject: "Asleep after completing DA_WCR_111022 assays" @23:59:05.21
Uploading Logs @00:00:27.46UTC11-Nov-22 upsync @20:14:14.88UTC11-Nov-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @20:14:17.53 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @20:14:21.59 Polling Can every 10:00 @20:14:21.69 Cleanly exited at 20:12:03UTC11-Nov-22 @20:14:21.79 Loading /home/esp/esp2/mission/DA_WCR_111122.rb mission @20:14:21.84 GoodNews.email "Starting DA_WCR_111122 w/pucks in tube 2\n{1 => 28,\n 2 => 5,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:14:21, 23.7C, 28% humidity, 15.7psia, 14.031V, 0.306A, 0.300A avg, 4.29W",Subject: "DA_WCR_111122 begun" @20:14:21.88
Preparing to sleep until + 00:00:05 @20:14:21.91 Asleep til + 00:00:05 @20:14:21.93 Continuing at 20:14 @20:14:22.21 Gate.power :core,:OFF @20:14:22.23 Gate.power :raw,:OFF Shutdown @20:14:26.18 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_111122.rb:14\n{1 => 28,\n 2 => 5,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:14:21, 23.7C, 28% humidity, 15.7psia, 14.031V, 0.306A, 0.300A avg, 4.29W",Subject: "Asleep until 20:14" @20:14:26.77
Powering core bus @20:14:32.51 Gate.power :raw,:ON @20:14:33.72 CS.configure :default @20:14:33.92 PS.configure :default @20:14:34.09 SS.configure :default @20:14:34.29 CC.configure :default @20:14:34.48 PC.configure :default @20:14:34.76 Forearm.configure :loose @20:14:34.93 Elbow.configure :loose @20:14:35.15 SC.configure :loose @20:14:35.35 SE.configure :default @20:14:35.43 CH.coast @20:14:35.55 CH.configure :default @20:14:35.68 PH.coast @20:14:35.78 PH.configure :default @20:14:35.86 All dwarf controllers running firmware version 3.85 @20:14:35.99 Valve.reset @20:14:36.49 Intake.select :close @20:14:36.56 Exhaust.select :close @20:14:36.59 CTV.dial :air @20:14:36.77 PSV.dial :air @20:14:36.87 CSV.dial :waste1 @20:14:37.11
Forearm.reconfigure :loose @20:14:37.19 Forearm.home.seek :retract @20:14:37.39 SSV.dial :spit,avoiding: :exhaust @20:14:37.92 PS.reconfigure :default @20:14:38.02 PS.home.seek 0ml @20:14:38.19 Hand.close @20:14:38.32
Elbow.jog 1500 @20:14:38.66 CS.reconfigure :fast @20:14:38.76 SS.reconfigure :default @20:14:38.92 CS.home.seek 0ml @20:14:39.00 SS.home.seek 0ml @20:14:39.22
Elbow.reconfigure :loose @20:14:39.30 Elbow.home.seek :clear @20:14:42.59 SE.reconfigure :default @20:14:42.67 SE.home.seek :down @20:14:43.41 SC.reconfigure :tight @20:14:43.49 SC.home.seek :initial @20:14:46.63 PS.stop @20:14:59.66
SC.configure :limp @20:14:59.76 Forearm.seek :retract @20:14:59.86 Elbow.seek :hammer @20:15:01.07 Forearm.reconfigure :tight @20:15:01.17 Forearm.seek retracted @20:15:01.64 PC.reconfigure :default @20:15:01.70
CC.reconfigure :default @20:15:01.84 PC.home.seek :open @20:15:01.90
CC.home.seek :open @20:15:05.77 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @20:15:05.79 Notice.email "At 20:15:05 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_111122.rb:14\n{1 => 28,\n 2 => 5,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:15:05, 23.7C, 28% humidity, 15.7psia, 14.036V, 0.239A, 0.245A avg, 3.35W",Subject: "Started DA_WCR_111122 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @20:15:05.83
Starting initialPurge @20:15:05.89 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:15:05.99 Hand.open @20:15:07.04 Elbow.reconfigure :tight @20:15:07.14 Elbow.seek FlushPuck::Garage @20:15:09.17 Elbow.reconfigure :loose @20:15:09.40 Forearm.reconfigure :loose @20:15:09.49 Forearm.seek :garage @20:15:10.76 Hand.close @20:15:11.63 Forearm.seek :retract @20:15:12.83 Elbow.reconfigure :tight @20:15:12.91 Elbow.seek CC @20:15:15.94 Elbow.reconfigure :loose @20:15:16.18 Forearm.reconfigure :tight @20:15:16.26 Forearm.seek CC @20:15:17.87 CC.seek :closed @20:15:22.26 Collection Clamp onSpring/offSpring for 8/25 samples, on/off torque 1.88 @20:15:22.48 Hand.open @20:15:23.42 Forearm.reconfigure :loose @20:15:23.50 Forearm.seek :retract @20:15:24.54 Flushing the Collection Valve::Manifold @20:15:24.76 CSV.dial CSR @20:15:26.17 Pulling 5ml of CSR.flush @20:15:26.19 CSR.select :flush @20:15:26.39 CS.reconfigure :default @20:15:26.46 CS.seek 5ml @20:15:39.96 delay 5 seconds @20:15:44.97 CSR.select :air @20:15:45.20 CS.reconfigure :fast @20:15:45.28 CS.seek 7.5ml @20:15:51.78 CSV.dial :waste @20:15:53.08 CS.reconfigure :default @20:15:53.14 CS.seek 0ml @20:16:12.84 Purging external Intake @20:16:13.08 CSV.dial :flush @20:16:13.56 CS.reconfigure :fast @20:16:13.64 CS.seek 10ml @20:16:29.23 upsync @20:16:36.26
delay 5 seconds @20:16:41.28 CSV.dial CBV @20:16:41.32
CBV.dial CSV @20:16:42.04 CTV.dial :intake @20:16:42.76 Intake.select :open @20:16:42.87 CS.seek 0ml @20:17:05.41 Intake.select :close @20:17:05.51 CTV.dial :air @20:17:06.25 Flushing upper manifold @20:17:06.36 CSV.dial :flush @20:17:07.08 CS.seek 5ml @20:17:18.85 delay 5 seconds @20:17:23.87 CSV.dial :air @20:17:24.36 CS.seek 7ml @20:17:29.55 CSV.dial CBV @20:17:29.57
CBV.dial CSV @20:17:30.68 CTV.dial :air @20:17:30.70 CS.seek 0ml @20:17:46.73 delay 7 seconds @20:17:53.75 CS.seek 3ml @20:18:01.04 CSV.dial :waste @20:18:01.47 CS.seek 0ml @20:18:09.02 CSV.dial :flush @20:18:09.43 CS.seek 3ml @20:18:16.91 delay 5 seconds @20:18:21.93 CSV.dial :air @20:18:22.42 CS.seek 5ml @20:18:27.60 CSV.dial :air @20:18:27.62 CS.seek 0ml @20:18:39.29 delay 7 seconds @20:18:46.31 CS.seek 3ml @20:18:53.66 CSV.dial :waste @20:18:54.37 CS.seek 0ml @20:19:01.73 Garaging FlushPuck in Collection Clamp @20:19:01.79 Elbow.reconfigure :tight @20:19:01.83 Elbow.seek CC @20:19:01.94 Elbow.reconfigure :loose @20:19:02.02 Forearm.seek CC @20:19:03.01 Hand.close @20:19:03.72 CC.seek :open @20:19:08.05 Forearm.seek :retract @20:19:09.06 Elbow.reconfigure :tight @20:19:09.11 Elbow.seek :garage @20:19:12.21 Elbow.reconfigure :loose @20:19:12.29 Forearm.reconfigure :tight @20:19:12.33 Forearm.seek :garage @20:19:13.44 Hand.open @20:19:14.28 Forearm.reconfigure :loose @20:19:14.73 Forearm.seek :retract @20:19:15.88 da sampling at most 1000ml, wcr at most 1000ml @20:19:15.91 Loading sh1 puck from tube 2 into Collection Clamp @20:19:15.92 DAfans.email "Started da in tube 2 at 20:19:15UTC11-Nov-22\n{1 => 28,\n 2 => 5,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:15:05, 23.7C, 28% humidity, 15.7psia, 14.036V, 0.239A, 0.245A avg, 3.35W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @20:19:16.17
SC.reconfigure :tight @20:19:16.21 SC.seek 2 @20:19:29.27 SC.configure :limp @20:19:29.69 Elbow.reconfigure :tight @20:19:29.91 Elbow.seek 2 @20:19:32.06 Forearm.reconfigure :tight @20:19:32.14 Forearm.seek 2 + 700 counts @20:19:33.37 SE.reconfigure :pick @20:19:33.47 SE.seek :beamBreak @20:19:36.38 SE.configure :default @20:19:36.76 Elbow.seek 2 @20:19:37.09 Forearm.seek 2 + 300 counts @20:19:37.39 Forearm.reconfigure :loose @20:19:37.47 Hand.close @20:19:38.15 SE.seek :down @20:19:38.53
Forearm.seek :retract @20:19:39.58 Elbow.seek CC @20:19:40.83 SE.stop @20:19:41.25
Elbow.reconfigure :loose @20:19:41.45 Forearm.reconfigure :tight @20:19:41.51 Forearm.seek CC @20:19:43.15 CC.seek :closed @20:19:47.51 Collection Clamp onSpring/offSpring for 9/24 samples, on/off torque 1.72 @20:19:47.53 Hand.open @20:19:48.48 Forearm.reconfigure :loose @20:19:48.57 Forearm.seek :retract @20:19:49.72 collectSample 1000ml w/bubble point of 24psi @20:19:49.74 Priming 7ml @20:19:49.76 Intake.select :close @20:19:49.78 Exhaust.select :close @20:19:49.80 SS.hold @20:19:50.25 Calibrating pressure and temperature sensors @20:19:50.31 CTV.dial :air @20:19:50.33 SSV.dial :spit,avoiding: :exhaust @20:19:50.37 SS.reconfigure :fast @20:19:50.45 SS.seek 5ml @20:19:53.73 SSV.dial CBV @20:19:53.78
CBV.dial SSV @20:19:55.16 delay 3.8 seconds @20:19:59.15 SSV.dial :spit @20:20:00.47 SS.seek 0ml @20:20:03.18 SSV.dial CBV @20:20:04.50 CTV.dial :intake @20:20:05.29 SSV.dial CBV @20:20:05.35
CBV.dial SSV @20:20:05.37 Allowing 40s for 7.0ml of remaining 7.0ml @20:20:05.39 Intake.select :open @20:20:07.66 Intake @15.1psia, vacuum from 9.6 to 12.6psi @20:20:07.70 SS.reconfigure :inhaling 2.14ml/s @20:20:07.79 SS.seek 7ml @20:20:13.53 Stroke completed -- waiting for pressure to equalize ... @20:20:13.55 SS.reconfigure :exhale @20:20:13.63 SS.hold @20:20:14.05 Intake.select :close @20:20:14.30 SSV.dial :exhaust @20:20:15.18 Exhaust.select :open @20:20:15.34 SS.seek 0ml @20:20:19.69 Exhaust.select :close @20:20:19.85 Sampling 1000.0ml @20:20:19.87 Intake.select :close @20:20:19.89 Exhaust.select :close @20:20:19.91 SS.reconfigure :default @20:20:19.99 SS.hold @20:20:20.39 CTV.dial :intake @20:20:20.41 SSV.dial CBV @20:20:20.47
CBV.dial SSV @20:20:21.26 Allowing 130s for 25.0ml of remaining 1000.0ml @20:20:21.28 Intake.select :open @20:20:23.57 Intake @15.2psia, vacuum from 9.7 to 12.7psi @20:20:23.59 SS.reconfigure :inhaling 2.14ml/s @20:20:23.68 SS.seek 25ml @20:20:29.34 Vacuum too high -- waiting up to 40 seconds @20:20:29.36 SS.reconfigure :exhale @20:20:29.46 SS.hold @20:20:30.21 Intake @10.2psia, vacuum from 4.7 to 7.7psi @20:20:30.27 SS.reconfigure :inhaling 1.93ml/s @20:20:30.35 SS.seek 25ml @20:20:34.06 Vacuum too high -- waiting up to 40 seconds @20:20:34.08 SS.reconfigure :exhale @20:20:34.16 SS.hold @20:20:35.22 Intake @11.8psia, vacuum from 6.3 to 9.3psi @20:20:35.26 SS.reconfigure :inhaling 1.74ml/s @20:20:35.32 SS.seek 25ml @20:20:41.04 Vacuum too high -- waiting up to 40 seconds @20:20:41.06 SS.reconfigure :exhale @20:20:41.14 SS.hold @20:20:42.65 Intake @14.0psia, vacuum from 8.5 to 11.5psi @20:20:42.69 SS.reconfigure :inhaling 1.57ml/s @20:20:42.78 SS.seek 25ml @20:20:47.30 Stroke completed -- waiting for pressure to equalize ... @20:20:47.32 SS.reconfigure :exhale @20:20:47.38 SS.hold @20:20:47.93 Intake.select :close @20:20:48.15 SSV.dial :exhaust @20:20:49.39 Exhaust.select :open @20:20:49.55 SS.seek 0ml @20:20:58.76 Exhaust.select :close @20:20:58.92 SSV.dial CBV @20:20:59.04
CBV.dial SSV @20:20:59.84 Allowing 130s for 25.0ml of remaining 975.0ml @20:20:59.86 Intake.select :open @20:21:02.11 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:21:02.13 SS.reconfigure :inhaling 1.57ml/s @20:21:02.30 SS.seek 25ml @20:21:12.34 Vacuum too high -- waiting up to 40 seconds @20:21:12.36 SS.reconfigure :exhale @20:21:12.44 SS.hold @20:21:13.07 Intake @10.5psia, vacuum from 5.0 to 8.0psi @20:21:13.11 SS.reconfigure :inhaling 1.41ml/s @20:21:13.21 SS.seek 25ml @20:21:22.15 upsync @20:21:22.79
Stroke completed -- waiting for pressure to equalize ... @20:21:22.80 SS.reconfigure :exhale @20:21:22.84 SS.hold @20:21:23.40 Intake.select :close @20:21:23.54 SSV.dial :exhaust @20:21:24.26 Exhaust.select :open @20:21:24.37 SS.seek 0ml @20:21:33.21 Exhaust.select :close @20:21:33.32 SSV.dial CBV @20:21:33.34
CBV.dial SSV @20:21:34.07 Allowing 130s for 25.0ml of remaining 950.0ml @20:21:34.08 Intake.select :open @20:21:36.24 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:21:36.25 SS.reconfigure :inhaling 1.41ml/s @20:21:36.30 SS.seek 25ml @20:21:56.79 Stroke completed -- waiting for pressure to equalize ... @20:21:56.80 SS.reconfigure :exhale @20:21:56.84 SS.hold @20:21:57.70 Intake.select :close @20:21:57.84 SSV.dial :exhaust @20:21:58.57 Exhaust.select :open @20:21:58.68 SS.seek 0ml @20:22:07.85 Exhaust.select :close @20:22:07.95 SSV.dial CBV @20:22:07.98
CBV.dial SSV @20:22:08.73 Allowing 130s for 25.0ml of remaining 925.0ml @20:22:08.74 Intake.select :open @20:22:10.89 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:22:10.91 SS.reconfigure :inhaling 1.41ml/s @20:22:10.95 SS.seek 25ml @20:22:30.55 Stroke completed -- waiting for pressure to equalize ... @20:22:30.56 SS.reconfigure :exhale @20:22:30.60 SS.hold @20:22:31.46 Intake.select :close @20:22:31.60 SSV.dial :exhaust @20:22:32.32 Exhaust.select :open @20:22:32.43 SS.seek 0ml @20:22:41.60 Exhaust.select :close @20:22:41.70 SSV.dial CBV @20:22:41.73
CBV.dial SSV @20:22:42.46 Allowing 130s for 25.0ml of remaining 900.0ml @20:22:42.47 Intake.select :open @20:22:44.62 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:22:44.64 SS.reconfigure :inhaling 1.41ml/s @20:22:44.68 SS.seek 25ml @20:23:04.32 Stroke completed -- waiting for pressure to equalize ... @20:23:04.33 SS.reconfigure :exhale @20:23:04.37 SS.hold @20:23:05.55 Intake.select :close @20:23:05.68 SSV.dial :exhaust @20:23:06.41 Exhaust.select :open @20:23:06.52 SS.seek 0ml @20:23:15.66 Exhaust.select :close @20:23:15.77 SSV.dial CBV @20:23:15.79
CBV.dial SSV @20:23:16.54 Allowing 130s for 25.0ml of remaining 875.0ml @20:23:16.55 Intake.select :open @20:23:18.70 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:23:18.72 SS.reconfigure :inhaling 1.41ml/s @20:23:18.76 SS.seek 25ml @20:23:38.25 Stroke completed -- waiting for pressure to equalize ... @20:23:38.26 SS.reconfigure :exhale @20:23:38.31 SS.hold @20:23:39.49 Intake.select :close @20:23:39.62 SSV.dial :exhaust @20:23:40.35 Exhaust.select :open @20:23:40.46 SS.seek 0ml @20:23:49.41 Exhaust.select :close @20:23:49.51 SSV.dial CBV @20:23:49.54
CBV.dial SSV @20:23:50.27 Allowing 130s for 25.0ml of remaining 850.0ml @20:23:50.28 Intake.select :open @20:23:52.43 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:23:52.45 SS.reconfigure :inhaling 1.41ml/s @20:23:52.49 SS.seek 25ml @20:24:12.92 Stroke completed -- waiting for pressure to equalize ... @20:24:12.93 SS.reconfigure :exhale @20:24:12.97 SS.hold @20:24:14.15 Intake.select :close @20:24:14.29 SSV.dial :exhaust @20:24:15.01 Exhaust.select :open @20:24:15.11 SS.seek 0ml @20:24:23.93 Exhaust.select :close @20:24:24.03 SSV.dial CBV @20:24:24.06
CBV.dial SSV @20:24:24.79 Allowing 130s for 25.0ml of remaining 825.0ml @20:24:24.80 Intake.select :open @20:24:26.95 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:24:26.96 SS.reconfigure :inhaling 1.41ml/s @20:24:27.01 SS.seek 25ml @20:24:47.50 Stroke completed -- waiting for pressure to equalize ... @20:24:47.51 SS.reconfigure :exhale @20:24:47.55 SS.hold @20:24:48.73 Intake.select :close @20:24:48.88 SSV.dial :exhaust @20:24:49.60 Exhaust.select :open @20:24:49.71 SS.seek 0ml @20:24:58.86 Exhaust.select :close @20:24:58.96 SSV.dial CBV @20:24:58.99
CBV.dial SSV @20:24:59.72 Allowing 130s for 25.0ml of remaining 800.0ml @20:24:59.73 Intake.select :open @20:25:01.88 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:25:01.90 SS.reconfigure :inhaling 1.41ml/s @20:25:01.94 SS.seek 25ml @20:25:21.52 Stroke completed -- waiting for pressure to equalize ... @20:25:21.53 SS.reconfigure :exhale @20:25:22.03 SS.hold @20:25:22.82 Intake.select :close @20:25:22.96 SSV.dial :exhaust @20:25:23.68 Exhaust.select :open @20:25:23.78 SS.seek 0ml @20:25:32.66 Exhaust.select :close @20:25:32.76 SSV.dial CBV @20:25:32.79
CBV.dial SSV @20:25:33.52 Allowing 130s for 25.0ml of remaining 775.0ml @20:25:33.53 Intake.select :open @20:25:35.68 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:25:35.70 SS.reconfigure :inhaling 1.41ml/s @20:25:35.74 SS.seek 25ml @20:25:56.22 Stroke completed -- waiting for pressure to equalize ... @20:25:56.23 SS.reconfigure :exhale @20:25:56.27 SS.hold @20:25:57.82 Intake.select :close @20:25:57.96 SSV.dial :exhaust @20:25:58.69 Exhaust.select :open @20:25:58.80 SS.seek 0ml @20:26:08.14 Exhaust.select :close @20:26:08.24 SSV.dial CBV @20:26:08.27
CBV.dial SSV @20:26:09.02 Allowing 130s for 25.0ml of remaining 750.0ml @20:26:09.03 Intake.select :open @20:26:11.18 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:26:11.20 SS.reconfigure :inhaling 1.41ml/s @20:26:11.24 SS.seek 25ml @20:26:30.79 Stroke completed -- waiting for pressure to equalize ... @20:26:30.80 SS.reconfigure :exhale @20:26:30.85 SS.hold @20:26:32.40 Intake.select :close @20:26:32.54 SSV.dial :exhaust @20:26:33.27 Exhaust.select :open @20:26:33.37 SS.seek 0ml @20:26:42.56 Exhaust.select :close @20:26:42.66 SSV.dial CBV @20:26:42.69
CBV.dial SSV @20:26:43.42 Allowing 130s for 25.0ml of remaining 725.0ml @20:26:43.43 Intake.select :open @20:26:45.59 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:26:45.60 SS.reconfigure :inhaling 1.41ml/s @20:26:45.65 SS.seek 25ml @20:27:05.25 Stroke completed -- waiting for pressure to equalize ... @20:27:05.26 SS.reconfigure :exhale @20:27:05.30 SS.hold @20:27:06.84 Intake.select :close @20:27:07.66 SSV.dial :exhaust @20:27:08.39 Exhaust.select :open @20:27:08.50 SS.seek 0ml @20:27:17.36 Exhaust.select :close @20:27:17.46 SSV.dial CBV @20:27:17.49
CBV.dial SSV @20:27:18.23 Allowing 130s for 25.0ml of remaining 700.0ml @20:27:18.24 Intake.select :open @20:27:20.40 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:27:20.41 SS.reconfigure :inhaling 1.41ml/s @20:27:20.45 SS.seek 25ml @20:27:40.92 Stroke completed -- waiting for pressure to equalize ... @20:27:40.93 SS.reconfigure :exhale @20:27:40.97 SS.hold @20:27:42.70 Intake.select :close @20:27:42.83 SSV.dial :exhaust @20:27:43.56 Exhaust.select :open @20:27:43.67 SS.seek 0ml @20:27:52.84 Exhaust.select :close @20:27:52.94 SSV.dial CBV @20:27:52.97
CBV.dial SSV @20:27:53.70 Allowing 130s for 25.0ml of remaining 675.0ml @20:27:53.71 Intake.select :open @20:27:55.86 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:27:55.88 SS.reconfigure :inhaling 1.41ml/s @20:27:55.92 SS.seek 25ml @20:28:15.45 Stroke completed -- waiting for pressure to equalize ... @20:28:15.46 SS.reconfigure :exhale @20:28:15.50 SS.hold @20:28:17.04 Intake.select :close @20:28:17.18 SSV.dial :exhaust @20:28:17.89 Exhaust.select :open @20:28:17.99 SS.seek 0ml @20:28:26.96 Exhaust.select :close @20:28:27.07 SSV.dial CBV @20:28:27.09
CBV.dial SSV @20:28:27.83 Allowing 130s for 25.0ml of remaining 650.0ml @20:28:27.84 Intake.select :open @20:28:29.99 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:28:30.00 SS.reconfigure :inhaling 1.41ml/s @20:28:30.05 SS.seek 25ml @20:28:50.48 Stroke completed -- waiting for pressure to equalize ... @20:28:50.49 SS.reconfigure :exhale @20:28:50.53 SS.hold @20:28:52.56 Intake.select :close @20:28:52.70 SSV.dial :exhaust @20:28:53.43 Exhaust.select :open @20:28:53.54 SS.seek 0ml @20:29:02.42 Exhaust.select :close @20:29:02.52 SSV.dial CBV @20:29:02.55
CBV.dial SSV @20:29:03.29 Allowing 130s for 25.0ml of remaining 625.0ml @20:29:03.30 Intake.select :open @20:29:05.45 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:29:05.47 SS.reconfigure :inhaling 1.41ml/s @20:29:05.51 SS.seek 25ml @20:29:26.02 Stroke completed -- waiting for pressure to equalize ... @20:29:26.03 SS.reconfigure :exhale @20:29:26.08 SS.hold @20:29:28.02 Intake.select :close @20:29:28.16 SSV.dial :exhaust @20:29:28.87 Exhaust.select :open @20:29:28.98 SS.seek 0ml @20:29:37.99 Exhaust.select :close @20:29:38.10 SSV.dial CBV @20:29:38.12
CBV.dial SSV @20:29:39.34 Allowing 130s for 25.0ml of remaining 600.0ml @20:29:39.35 Intake.select :open @20:29:41.51 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:29:41.53 SS.reconfigure :inhaling 1.41ml/s @20:29:41.57 SS.seek 25ml @20:29:59.72 Vacuum too high -- waiting up to 40 seconds @20:29:59.73 SS.reconfigure :exhale @20:29:59.77 SS.hold @20:30:00.27 Intake @10.1psia, vacuum from 4.6 to 7.6psi @20:30:00.28 SS.reconfigure :inhaling 1.27ml/s @20:30:00.33 SS.seek 25ml @20:30:02.80 Stroke completed -- waiting for pressure to equalize ... @20:30:02.81 SS.reconfigure :exhale @20:30:02.86 SS.hold @20:30:04.13 Intake.select :close @20:30:04.27 SSV.dial :exhaust @20:30:04.99 Exhaust.select :open @20:30:05.10 SS.seek 0ml @20:30:14.07 Exhaust.select :close @20:30:14.17 SSV.dial CBV @20:30:14.20
CBV.dial SSV @20:30:14.93 Allowing 130s for 25.0ml of remaining 575.0ml @20:30:14.94 Intake.select :open @20:30:17.10 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:30:17.11 SS.reconfigure :inhaling 1.27ml/s @20:30:17.15 SS.seek 25ml @20:30:39.58 Stroke completed -- waiting for pressure to equalize ... @20:30:39.59 SS.reconfigure :exhale @20:30:39.64 SS.hold @20:30:41.27 Intake.select :close @20:30:41.66 SSV.dial :exhaust @20:30:42.37 Exhaust.select :open @20:30:42.47 SS.seek 0ml @20:30:51.71 Exhaust.select :close @20:30:51.81 SSV.dial CBV @20:30:51.84
CBV.dial SSV @20:30:52.57 Allowing 130s for 25.0ml of remaining 550.0ml @20:30:52.58 Intake.select :open @20:30:54.73 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:30:54.75 SS.reconfigure :inhaling 1.27ml/s @20:30:54.79 SS.seek 25ml @20:31:16.27 Stroke completed -- waiting for pressure to equalize ... @20:31:16.28 SS.reconfigure :exhale @20:31:16.32 SS.hold @20:31:18.27 Intake.select :close @20:31:18.41 SSV.dial :exhaust @20:31:19.13 Exhaust.select :open @20:31:19.24 SS.seek 0ml @20:31:28.55 Exhaust.select :close @20:31:28.65 SSV.dial CBV @20:31:28.69
CBV.dial SSV @20:31:29.41 Allowing 130s for 25.0ml of remaining 525.0ml @20:31:29.42 Intake.select :open @20:31:31.57 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:31:31.59 SS.reconfigure :inhaling 1.27ml/s @20:31:31.63 SS.seek 25ml @20:31:53.07 Stroke completed -- waiting for pressure to equalize ... @20:31:53.08 SS.reconfigure :exhale @20:31:53.12 SS.hold @20:31:55.15 Intake.select :close @20:31:55.28 SSV.dial :exhaust @20:31:56.01 Exhaust.select :open @20:31:56.11 SS.seek 0ml @20:32:05.55 Exhaust.select :close @20:32:05.65 SSV.dial CBV @20:32:05.68
CBV.dial SSV @20:32:06.43 Allowing 130s for 25.0ml of remaining 500.0ml @20:32:06.44 Intake.select :open @20:32:08.59 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:32:08.61 SS.reconfigure :inhaling 1.27ml/s @20:32:08.65 SS.seek 25ml @20:32:30.36 Stroke completed -- waiting for pressure to equalize ... @20:32:30.37 SS.reconfigure :exhale @20:32:30.41 SS.hold @20:32:32.80 Intake.select :close @20:32:32.93 SSV.dial :exhaust @20:32:33.67 Exhaust.select :open @20:32:33.78 SS.seek 0ml @20:32:42.78 Exhaust.select :close @20:32:42.89 SSV.dial CBV @20:32:42.91
CBV.dial SSV @20:32:43.65 Allowing 130s for 25.0ml of remaining 475.0ml @20:32:43.66 Intake.select :open @20:32:45.81 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:32:45.82 SS.reconfigure :inhaling 1.27ml/s @20:32:45.86 SS.seek 25ml @20:33:07.31 Vacuum too high -- waiting up to 40 seconds @20:33:07.32 SS.reconfigure :exhale @20:33:07.36 SS.hold @20:33:07.81 Intake @10.7psia, vacuum from 5.2 to 8.2psi @20:33:07.82 SS.reconfigure :inhaling 1.14ml/s @20:33:07.87 SS.seek 25ml @20:33:09.66 Stroke completed -- waiting for pressure to equalize ... @20:33:09.67 SS.reconfigure :exhale @20:33:09.71 SS.hold @20:33:10.94 Intake.select :close @20:33:11.08 SSV.dial :exhaust @20:33:11.80 Exhaust.select :open @20:33:11.91 SS.seek 0ml @20:33:21.19 Exhaust.select :close @20:33:21.29 SSV.dial CBV @20:33:21.32
CBV.dial SSV @20:33:22.07 Allowing 130s for 25.0ml of remaining 450.0ml @20:33:22.08 Intake.select :open @20:33:24.23 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:33:24.25 SS.reconfigure :inhaling 1.14ml/s @20:33:24.29 SS.seek 25ml @20:33:47.91 Stroke completed -- waiting for pressure to equalize ... @20:33:47.92 SS.reconfigure :exhale @20:33:47.96 SS.hold @20:33:49.91 Intake.select :close @20:33:50.04 SSV.dial :exhaust @20:33:50.77 Exhaust.select :open @20:33:50.88 SS.seek 0ml @20:33:59.84 Exhaust.select :close @20:33:59.95 SSV.dial CBV @20:33:59.97
CBV.dial SSV @20:34:00.71 Allowing 130s for 25.0ml of remaining 425.0ml @20:34:00.72 Intake.select :open @20:34:02.87 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:34:02.88 SS.reconfigure :inhaling 1.14ml/s @20:34:02.92 SS.seek 25ml @20:34:27.48 Stroke completed -- waiting for pressure to equalize ... @20:34:27.49 SS.reconfigure :exhale @20:34:27.54 SS.hold @20:34:30.02 Intake.select :close @20:34:30.16 SSV.dial :exhaust @20:34:30.89 Exhaust.select :open @20:34:31.00 SS.seek 0ml @20:34:40.40 Exhaust.select :close @20:34:40.51 SSV.dial CBV @20:34:40.54
CBV.dial SSV @20:34:41.28 Allowing 130s for 25.0ml of remaining 400.0ml @20:34:41.29 Intake.select :open @20:34:43.44 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:34:43.46 SS.reconfigure :inhaling 1.14ml/s @20:34:43.50 SS.seek 25ml @20:35:07.20 Stroke completed -- waiting for pressure to equalize ... @20:35:07.21 SS.reconfigure :exhale @20:35:07.25 SS.hold @20:35:10.14 Intake.select :close @20:35:10.28 SSV.dial :exhaust @20:35:11.00 Exhaust.select :open @20:35:11.11 SS.seek 0ml @20:35:20.08 Exhaust.select :close @20:35:20.18 SSV.dial CBV @20:35:20.21
CBV.dial SSV @20:35:20.94 Allowing 130s for 25.0ml of remaining 375.0ml @20:35:20.95 Intake.select :open @20:35:23.10 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:35:23.12 SS.reconfigure :inhaling 1.14ml/s @20:35:23.16 SS.seek 25ml @20:35:40.02 Vacuum too high -- waiting up to 40 seconds @20:35:40.03 SS.reconfigure :exhale @20:35:40.07 SS.hold @20:35:40.45 Intake @11.9psia, vacuum from 6.4 to 9.4psi @20:35:40.47 SS.reconfigure :inhaling 1.03ml/s @20:35:40.51 SS.seek 25ml @20:35:49.46 Stroke completed -- waiting for pressure to equalize ... @20:35:49.47 SS.reconfigure :exhale @20:35:49.51 SS.hold @20:35:51.45 Intake.select :close @20:35:51.59 SSV.dial :exhaust @20:35:52.31 Exhaust.select :open @20:35:52.42 SS.seek 0ml @20:36:01.78 Exhaust.select :close @20:36:01.88 SSV.dial CBV @20:36:01.92
CBV.dial SSV @20:36:02.62 Allowing 130s for 25.0ml of remaining 350.0ml @20:36:02.63 Intake.select :open @20:36:04.78 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:36:04.80 SS.reconfigure :inhaling 1.03ml/s @20:36:04.85 SS.seek 25ml @20:36:30.85 Stroke completed -- waiting for pressure to equalize ... @20:36:30.86 SS.reconfigure :exhale @20:36:30.90 SS.hold @20:36:33.40 Intake.select :close @20:36:33.54 SSV.dial :exhaust @20:36:34.28 Exhaust.select :open @20:36:34.38 SS.seek 0ml @20:36:43.35 Exhaust.select :close @20:36:43.46 SSV.dial CBV @20:36:43.48
CBV.dial SSV @20:36:44.22 Allowing 130s for 25.0ml of remaining 325.0ml @20:36:44.23 Intake.select :open @20:36:46.38 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:36:46.40 SS.reconfigure :inhaling 1.03ml/s @20:36:46.44 SS.seek 25ml @20:37:13.40 Stroke completed -- waiting for pressure to equalize ... @20:37:13.41 SS.reconfigure :exhale @20:37:13.45 SS.hold @20:37:16.99 Intake.select :close @20:37:17.13 SSV.dial :exhaust @20:37:17.84 Exhaust.select :open @20:37:17.95 SS.seek 0ml @20:37:26.88 Exhaust.select :close @20:37:26.99 SSV.dial CBV @20:37:27.01
CBV.dial SSV @20:37:27.75 Allowing 130s for 25.0ml of remaining 300.0ml @20:37:27.76 Intake.select :open @20:37:29.91 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:37:29.92 SS.reconfigure :inhaling 1.03ml/s @20:37:29.97 SS.seek 25ml @20:37:48.11 Vacuum too high -- waiting up to 40 seconds @20:37:48.12 SS.reconfigure :exhale @20:37:48.16 SS.hold @20:37:48.82 Intake @12.2psia, vacuum from 6.7 to 9.7psi @20:37:48.84 SS.reconfigure :inhaling 0.93ml/s @20:37:48.88 SS.seek 25ml @20:37:59.21 Stroke completed -- waiting for pressure to equalize ... @20:37:59.22 SS.reconfigure :exhale @20:37:59.26 SS.hold @20:38:01.66 Intake.select :close @20:38:01.79 SSV.dial :exhaust @20:38:02.52 Exhaust.select :open @20:38:02.63 SS.seek 0ml @20:38:11.65 Exhaust.select :close @20:38:11.75 SSV.dial CBV @20:38:11.78
CBV.dial SSV @20:38:12.51 Allowing 130s for 25.0ml of remaining 275.0ml @20:38:12.52 Intake.select :open @20:38:14.67 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:38:14.69 SS.reconfigure :inhaling 0.93ml/s @20:38:14.73 SS.seek 25ml @20:38:44.24 Stroke completed -- waiting for pressure to equalize ... @20:38:44.25 SS.reconfigure :exhale @20:38:44.29 SS.hold @20:38:48.36 Intake.select :close @20:38:48.50 SSV.dial :exhaust @20:38:49.21 Exhaust.select :open @20:38:49.32 SS.seek 0ml @20:38:58.66 Exhaust.select :close @20:38:58.76 SSV.dial CBV @20:38:58.79
CBV.dial SSV @20:38:59.52 Allowing 130s for 25.0ml of remaining 250.0ml @20:38:59.53 Intake.select :open @20:39:01.68 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:39:01.70 SS.reconfigure :inhaling 0.93ml/s @20:39:01.74 SS.seek 25ml @20:39:23.11 Vacuum too high -- waiting up to 40 seconds @20:39:23.12 SS.reconfigure :exhale @20:39:23.16 SS.hold @20:39:24.43 Intake @12.5psia, vacuum from 7.0 to 10.0psi @20:39:24.45 SS.reconfigure :inhaling 0.84ml/s @20:39:24.49 SS.seek 25ml @20:39:32.92 Stroke completed -- waiting for pressure to equalize ... @20:39:32.93 SS.reconfigure :exhale @20:39:32.98 SS.hold @20:39:35.46 Intake.select :close @20:39:35.60 SSV.dial :exhaust @20:39:36.32 Exhaust.select :open @20:39:36.42 SS.seek 0ml @20:39:45.39 Exhaust.select :close @20:39:45.50 SSV.dial CBV @20:39:45.52
CBV.dial SSV @20:39:46.71 Allowing 130s for 25.0ml of remaining 225.0ml @20:39:46.72 Intake.select :open @20:39:48.87 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:39:48.89 SS.reconfigure :inhaling 0.84ml/s @20:39:48.93 SS.seek 25ml @20:40:21.17 Stroke completed -- waiting for pressure to equalize ... @20:40:21.18 SS.reconfigure :exhale @20:40:21.23 SS.hold @20:40:25.39 Intake.select :close @20:40:25.53 SSV.dial :exhaust @20:40:26.24 Exhaust.select :open @20:40:26.35 SS.seek 0ml @20:40:35.39 Exhaust.select :close @20:40:35.50 SSV.dial CBV @20:40:35.52
CBV.dial SSV @20:40:36.25 Allowing 130s for 25.0ml of remaining 200.0ml @20:40:36.27 Intake.select :open @20:40:38.42 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:40:38.43 SS.reconfigure :inhaling 0.84ml/s @20:40:38.47 SS.seek 25ml @20:41:06.67 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @20:41:06.72 SS.hold @20:41:09.19 Intake @13.4psia, vacuum from 7.9 to 10.9psi @20:41:09.21 SS.reconfigure :inhaling 0.76ml/s @20:41:09.26 SS.seek 25ml @20:41:14.20 Stroke completed -- waiting for pressure to equalize ... @20:41:14.21 SS.reconfigure :exhale @20:41:14.25 SS.hold @20:41:17.24 Intake.select :close @20:41:17.37 SSV.dial :exhaust @20:41:18.10 Exhaust.select :open @20:41:18.20 SS.seek 0ml @20:41:27.52 Exhaust.select :close @20:41:27.62 SSV.dial CBV @20:41:27.66
CBV.dial SSV @20:41:28.36 Allowing 130s for 25.0ml of remaining 175.0ml @20:41:28.37 Intake.select :open @20:41:30.52 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:41:30.54 SS.reconfigure :inhaling 0.76ml/s @20:41:30.58 SS.seek 25ml @20:42:03.63 Vacuum too high -- waiting up to 40 seconds @20:42:03.64 SS.reconfigure :exhale @20:42:03.68 SS.hold @20:42:05.31 Intake @13.2psia, vacuum from 7.7 to 10.7psi @20:42:05.33 SS.reconfigure :inhaling 0.69ml/s @20:42:05.37 SS.seek 25ml @20:42:07.53 Stroke completed -- waiting for pressure to equalize ... @20:42:07.54 SS.reconfigure :exhale @20:42:07.58 SS.hold @20:42:09.98 Intake.select :close @20:42:10.11 SSV.dial :exhaust @20:42:10.83 Exhaust.select :open @20:42:10.93 SS.seek 0ml @20:42:20.36 Exhaust.select :close @20:42:20.46 SSV.dial CBV @20:42:20.48
CBV.dial SSV @20:42:21.22 Allowing 130s for 25.0ml of remaining 150.0ml @20:42:21.23 Intake.select :open @20:42:23.38 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:42:23.40 SS.reconfigure :inhaling 0.69ml/s @20:42:23.44 SS.seek 25ml @20:43:00.29 Vacuum too high -- waiting up to 40 seconds @20:43:00.30 SS.reconfigure :exhale @20:43:00.34 SS.hold @20:43:02.82 Intake @13.9psia, vacuum from 8.4 to 11.4psi @20:43:02.84 SS.reconfigure :inhaling 0.61ml/s @20:43:02.88 SS.seek 25ml @20:43:04.97 Stroke completed -- waiting for pressure to equalize ... @20:43:04.98 SS.reconfigure :exhale @20:43:05.02 SS.hold @20:43:07.58 Intake.select :close @20:43:07.72 SSV.dial :exhaust @20:43:08.45 Exhaust.select :open @20:43:08.56 SS.seek 0ml @20:43:17.87 Exhaust.select :close @20:43:17.98 SSV.dial CBV @20:43:18.00
CBV.dial SSV @20:43:18.73 Allowing 130s for 25.0ml of remaining 125.0ml @20:43:18.75 Intake.select :open @20:43:20.90 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:43:20.91 SS.reconfigure :inhaling 0.61ml/s @20:43:20.96 SS.seek 25ml @20:44:03.73 Stroke completed -- waiting for pressure to equalize ... @20:44:03.74 SS.reconfigure :exhale @20:44:03.78 SS.hold @20:44:08.62 Intake.select :close @20:44:08.76 SSV.dial :exhaust @20:44:09.48 Exhaust.select :open @20:44:09.59 SS.seek 0ml @20:44:18.92 Exhaust.select :close @20:44:19.02 SSV.dial CBV @20:44:19.06
CBV.dial SSV @20:44:19.76 Allowing 130s for 25.0ml of remaining 100.0ml @20:44:19.77 Intake.select :open @20:44:21.92 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:44:21.94 SS.reconfigure :inhaling 0.61ml/s @20:44:21.98 SS.seek 25ml @20:45:05.04 Stroke completed -- waiting for pressure to equalize ... @20:45:05.05 SS.reconfigure :exhale @20:45:05.09 SS.hold @20:45:11.48 Intake.select :close @20:45:11.62 SSV.dial :exhaust @20:45:12.34 Exhaust.select :open @20:45:12.44 SS.seek 0ml @20:45:21.45 Exhaust.select :close @20:45:21.55 SSV.dial CBV @20:45:21.58
CBV.dial SSV @20:45:22.31 Allowing 130s for 25.0ml of remaining 75.0ml @20:45:22.32 Intake.select :open @20:45:24.47 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:45:24.49 SS.reconfigure :inhaling 0.61ml/s @20:45:24.53 SS.seek 25ml @20:45:57.31 Vacuum too high -- waiting up to 40 seconds @20:45:57.32 SS.reconfigure :exhale @20:45:57.36 SS.hold @20:46:00.35 Intake @13.9psia, vacuum from 8.4 to 11.4psi @20:46:00.36 SS.reconfigure :inhaling 0.56ml/s @20:46:00.41 SS.seek 25ml @20:46:13.29 Stroke completed -- waiting for pressure to equalize ... @20:46:13.30 SS.reconfigure :exhale @20:46:13.34 SS.hold @20:46:18.09 Intake.select :close @20:46:18.23 SSV.dial :exhaust @20:46:18.95 Exhaust.select :open @20:46:19.06 SS.seek 0ml @20:46:28.37 Exhaust.select :close @20:46:28.47 SSV.dial CBV @20:46:28.50
CBV.dial SSV @20:46:29.24 Allowing 130s for 25.0ml of remaining 50.0ml @20:46:29.25 Intake.select :open @20:46:31.40 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:46:31.41 SS.reconfigure :inhaling 0.56ml/s @20:46:31.46 SS.seek 25ml @20:47:19.41 Stroke completed -- waiting for pressure to equalize ... @20:47:19.42 SS.reconfigure :exhale @20:47:19.46 SS.hold @20:47:25.86 Intake.select :close @20:47:26.00 SSV.dial :exhaust @20:47:26.72 Exhaust.select :open @20:47:26.83 SS.seek 0ml @20:47:35.88 Exhaust.select :close @20:47:35.98 SSV.dial CBV @20:47:36.01
CBV.dial SSV @20:47:36.74 Allowing 130s for 25.0ml of remaining 25.0ml @20:47:36.75 Intake.select :open @20:47:38.90 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:47:38.92 SS.reconfigure :inhaling 0.56ml/s @20:47:38.96 SS.seek 24.95ml @20:48:16.09 Vacuum too high -- waiting up to 40 seconds @20:48:16.10 SS.reconfigure :exhale @20:48:16.14 SS.hold @20:48:19.58 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:48:19.59 SS.reconfigure :inhaling 0.50ml/s @20:48:19.64 SS.seek 24.95ml @20:48:33.97 Stroke completed -- waiting for pressure to equalize ... @20:48:33.98 SS.reconfigure :exhale @20:48:34.02 SS.hold @20:48:39.55 Intake.select :close @20:48:39.69 SSV.dial :exhaust @20:48:40.41 Exhaust.select :open @20:48:40.52 SS.seek 0ml @20:48:49.99 Exhaust.select :close @20:48:50.09 Sampled 1000.0ml @20:48:50.10 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @20:48:50.11 Intake.select :close @20:48:50.17 CSV.dial :waste @20:48:50.21 CS.seek 0ml @20:48:50.31 SSV.dial CBV,avoiding: :exhaust @20:48:50.33
CBV.dial SSV @20:48:50.39 CSV.dial :air @20:48:51.10 CS.seek 10ml @20:48:52.15
CTV.dial :air @20:48:52.88 delay 15 seconds @20:49:07.90 SSV.dial :spit,avoiding: :exhaust @20:49:09.18 CTV.dial CSV,avoiding: :intake @20:49:09.23
SS.seek 0ml @20:49:09.32 SSV.dial CBV,avoiding: :exhaust @20:49:13.80 CBV.dial between SSV and waste2 @20:49:14.17 CTV.dial CSV,avoiding: :air @20:49:14.20
CSV.dial CTV @20:49:14.63 CS.reconfigure :pressurize @20:49:14.68 CS.seek 0ml @20:49:33.42 BubblePoint: Intake @40.4psia, Outlet @16.2psia @20:49:33.43 CBV.dial SSV @20:49:33.75 SS.reconfigure :default @20:49:33.80 SS.seek 10ml @20:49:39.25 delay 15 seconds @20:49:54.37 Evacuated: Intake @17.0psia, Outlet @10.4psia @20:49:54.38 SSV.dial :spit,avoiding: :exhaust @20:49:55.68 SS.reconfigure :exhale @20:49:55.72 SS.seek 0ml @20:50:01.57 SS.reconfigure :fast @20:50:01.61 SS.seek 5ml @20:50:04.21 SSV.dial CBV,avoiding: :exhaust @20:50:04.25
CBV.dial SSV @20:50:05.50 CTV.dial :air,avoiding: :intake @20:50:06.75 CSV.dial CTV @20:50:06.79
SS.seek 10ml @20:50:07.78 CSV.dial :air @20:50:08.21 CS.reconfigure :fast @20:50:08.25 CS.seek 0ml @20:50:09.47
SSV.dial :spit,avoiding: :exhaust @20:50:10.76 SS.reconfigure :exhale @20:50:10.80 SS.seek 0ml @20:50:15.11 CS.stop @20:50:16.26
SS.reconfigure :fast @20:50:16.30 SS.seek 5ml @20:50:18.90 SSV.dial CBV,avoiding: :exhaust @20:50:18.92
CBV.dial SSV @20:50:21.22 SSV.dial :spit,avoiding: :exhaust @20:50:22.52 SS.reconfigure :default @20:50:23.01 SS.seek 0ml @20:50:26.34 SS.stop @20:50:26.38 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @20:50:26.39 Intake.select :close @20:50:26.46 CSV.dial :waste @20:50:26.58 SSV.dial CBV,avoiding: :exhaust @20:50:26.61
CBV.dial SSV @20:50:27.22 CS.seek 0ml @20:50:27.30 CSV.dial :air @20:50:27.90
CTV.dial :air @20:50:27.91 delay 15 seconds @20:50:28.02 CS.seek 10ml @20:50:42.93
SSV.dial :spit,avoiding: :exhaust @20:50:44.21 CTV.dial CSV,avoiding: :intake @20:50:44.23
SS.reconfigure :exhale @20:50:44.33 SS.seek 0ml @20:50:44.38 SSV.dial CBV,avoiding: :exhaust @20:50:50.60 CBV.dial between SSV and waste2 @20:50:50.98 CTV.dial CSV,avoiding: :air @20:50:51.01
CSV.dial CTV @20:50:51.45 CS.reconfigure :pressurize @20:50:51.49 CS.seek 0ml @20:51:12.61 BubblePoint: Intake @39.4psia, Outlet @15.2psia @20:51:12.62 CBV.dial SSV @20:51:12.93 SS.reconfigure :default @20:51:12.97 SS.seek 10ml @20:51:18.50 delay 15 seconds @20:51:33.61 Evacuated: Intake @19.0psia, Outlet @12.2psia @20:51:33.62 SSV.dial :spit,avoiding: :exhaust @20:51:34.91 SS.reconfigure :exhale @20:51:34.95 SS.seek 0ml @20:51:40.41 SS.reconfigure :fast @20:51:40.45 SS.seek 5ml @20:51:43.24 SSV.dial CBV,avoiding: :exhaust @20:51:43.26
CBV.dial SSV @20:51:44.55 CTV.dial :air,avoiding: :intake @20:51:45.81 CSV.dial CTV @20:51:45.85
SS.seek 10ml @20:51:46.85 CSV.dial :air @20:51:47.28 CS.reconfigure :fast @20:51:47.33 CS.seek 0ml @20:51:48.36
SSV.dial :spit,avoiding: :exhaust @20:51:49.66 SS.reconfigure :exhale @20:51:49.70 SS.seek 0ml @20:51:52.20 CS.stop @20:51:55.10
SS.reconfigure :fast @20:51:55.14 SS.seek 5ml @20:51:58.14 SSV.dial CBV,avoiding: :exhaust @20:51:58.17
CBV.dial SSV @20:52:00.48 SSV.dial :spit,avoiding: :exhaust @20:52:01.75 SS.reconfigure :default @20:52:01.79 SS.seek 0ml @20:52:04.95 SS.stop @20:52:04.99 Lysing: Adding 2.0ml AqMeOH @20:52:05.00 CBV.dial between CSV and waste1,avoiding: :DBV @20:52:06.42 CTV.dial CSV @20:52:07.68 CSV.dial CSR @20:52:08.44 Pulling 1ml of CSR.AqMeOH @20:52:08.45 CSR.select :AqMeOH @20:52:08.57 CS.reconfigure :default @20:52:08.61 CS.seek 1ml @20:52:12.02 delay 5 seconds @20:52:17.03 CSR.select :air @20:52:17.19 CS.seek 6ml @20:52:30.67 delay 2 seconds @20:52:32.68 CSV.dial :waste2 @20:52:33.22 CS.reconfigure :fast @20:52:33.26 CS.seek 0ml @20:52:47.04 CSV.dial CSR @20:52:47.52 Pulling 2ml of CSR.AqMeOH @20:52:47.53 CSR.select :AqMeOH @20:52:47.64 CS.reconfigure :default @20:52:47.68 CS.seek 2ml @20:52:53.51 delay 5 seconds @20:52:58.53 CSR.select :air @20:52:58.68 CS.seek 4.999ml @20:53:07.13 delay 2 seconds @20:53:09.15 CSV.dial CTV @20:53:09.58 CS.seek 1ml @20:53:20.61 Before lysing Collection Puck Heater at 19.1C/38.7psia @20:53:20.63 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @20:53:20.65 CH.seek 70C,8:00 @20:55:05.87 CH.hold 10:00,70C @20:55:06.04 Begun lysing Collection Puck Heater at 68.1C/42.6psia @21:05:06.05 Ended lysing Collection Puck Heater at 70C/44.1psia @21:05:06.06 CS.seek 0ml @21:05:09.46 CTV.dial between spare and air,avoiding: :intake @21:05:10.43 CSV.dial :air @21:05:10.86 CS.seek 5ml @21:05:24.33 CSV.dial CTV @21:05:24.76 delay 2 seconds @21:05:26.77 CSV.dial :air @21:05:27.32 delay 2 seconds @21:05:29.33 CSV.dial CTV @21:05:29.76 delay 2 seconds @21:05:31.77 CSV.dial :air @21:05:32.19 delay 2 seconds @21:05:34.21 CS.seek 0ml @21:05:47.69 Puck @70.0psia after final lysis pressurization @21:05:47.71 Recovering Lysate into Collection syringe @21:05:47.72 CSV.dial CBV @21:05:48.69 CBV.dial CSV @21:05:49.05 CS.seek 5ml @21:06:02.49 delay 5 seconds @21:06:07.51 CTV.dial :air @21:06:08.00 CS.seek 7ml @21:06:13.86 delay 5 seconds @21:06:18.88 CSV.dial :air2 @21:06:19.32 CS.seek 10ml @21:06:27.68 CSV.dial between air and CTV,avoiding: CBV @21:06:28.79 Lysing Complete @21:06:28.86 Storing sh1 puck in Collection Clamp to tube 1 @21:06:29.02 SC.reconfigure :tight @21:06:29.06 SC.seek 1 @21:06:32.64 SC.configure :limp @21:06:32.84 Elbow.reconfigure :tight @21:06:32.88 Elbow.seek 1 @21:06:34.84 Forearm.reconfigure :tight @21:06:34.88 Forearm.seek 1 + 700 counts @21:06:35.90 SE.reconfigure :pick @21:06:35.95 SE.seek :beamBreak @21:06:37.22 SE.configure :default @21:06:37.36 Elbow.reconfigure :limp @21:06:37.41 Forearm.reconfigure :loose @21:06:37.44 Forearm.seek 1 @21:06:37.82 Elbow.hold @21:06:37.88 Elbow.reconfigure :tight @21:06:37.98 SE.seek 27.4 Pucks below Top Plate @21:06:38.44 Forearm.force -20 @21:06:38.47 Forearm.seek 1 + 315 counts @21:06:38.84 Forearm.reconfigure :tight @21:06:38.89 Forearm.seek 1 + 700 counts @21:06:39.25 SE.seek 28.4 Pucks below Top Plate @21:06:39.90 Forearm.reconfigure :loose @21:06:39.95 Forearm.seek :retract @21:06:40.94 Elbow.seek CC @21:06:42.45 Elbow.reconfigure :loose @21:06:43.03 Forearm.seek CC @21:06:43.96 Hand.close @21:06:44.62 CC.seek :open @21:06:49.05 Forearm.seek :retract @21:06:50.08 Elbow.reconfigure :tight @21:06:50.12 Elbow.seek 1 @21:06:51.78 Elbow.reconfigure :limp @21:06:51.82 Forearm.seek 1 @21:06:52.98 Elbow.hold @21:06:53.04 Elbow.reconfigure :tight @21:06:53.08 Hand.open @21:06:54.00 Forearm.force -20 @21:06:54.04 Forearm.seek 1 + 316 counts @21:06:54.33 Forearm.reconfigure :tight @21:06:54.37 Forearm.seek 1 + 700 counts @21:06:54.65 SE.seek :down @21:06:55.52 SE.stop @21:06:55.78
Forearm.reconfigure :loose @21:06:55.82 Forearm.seek :retract @21:06:56.75 Tranferring DA @21:06:56.76 Beginning samplePassC @21:06:56.77 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:06:56.95 Elbow.seek FlushPuck::Garage @21:06:58.70 Elbow.reconfigure :loose @21:06:58.79 Forearm.seek :garage @21:06:59.92 Hand.close @21:07:00.60 Forearm.seek :retract @21:07:01.68 Elbow.reconfigure :tight @21:07:01.73 Elbow.seek CC @21:07:04.66 Elbow.reconfigure :loose @21:07:04.74 Forearm.reconfigure :tight @21:07:04.78 Forearm.seek CC @21:07:06.01 CC.seek :closed @21:07:10.31 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.92 @21:07:10.38 Hand.open @21:07:11.18 Forearm.reconfigure :loose @21:07:11.22 Forearm.seek :retract @21:07:12.21 PSV.dial PSR @21:07:13.51 Pulling 1.5ml of PSR.flush @21:07:13.52 PSR.select :flush @21:07:13.64 PS.seek 1.5ml @21:07:29.70 delay 5 seconds @21:07:34.71 PSV.dial :waste1 @21:07:35.20 PS.seek 0ml @21:07:51.25 PSV.dial PSR @21:07:51.80 PSR.select :air @21:07:51.91 PS.seek 2.5ml @21:08:18.04 PRV.dial CBV,avoiding: :ab2 @21:08:18.07
CBV.dial PRV @21:08:19.69 PSV.dial :mixloop @21:08:20.69 CTV.dial :air,avoiding: :intake @21:08:20.71 PTV.dial :PRVmixing @21:08:21.93 PS.seek 0ml @21:08:48.16 PSV.dial :air @21:08:48.59 PS.seek 2.5ml @21:09:14.81 PSV.dial :mixloop @21:09:15.26 PS.seek 0ml @21:09:41.52 PSV.dial :air @21:09:41.99 PS.seek 2.5ml @21:10:08.34 PSV.dial :mixloop @21:10:08.79 PS.seek 0ml @21:10:35.00 Garaging FlushPuck in Collection Clamp @21:10:35.07 Elbow.reconfigure :tight @21:10:35.11 Elbow.seek CC @21:10:35.21 Elbow.reconfigure :loose @21:10:35.76 Forearm.seek CC @21:10:36.80 Hand.close @21:10:37.37 CC.seek :open @21:10:41.68 Forearm.seek :retract @21:10:42.66 Elbow.reconfigure :tight @21:10:42.70 Elbow.seek :garage @21:10:45.77 Elbow.reconfigure :loose @21:10:45.86 Forearm.reconfigure :tight @21:10:45.90 Forearm.seek :garage @21:10:47.00 Hand.open @21:10:47.80 Forearm.reconfigure :loose @21:10:47.84 Forearm.seek :retract @21:10:48.91 PSV.dial :mixloop @21:10:48.92 PTV.dial :PRVmixing @21:10:48.93 PRV.dial CBV @21:10:48.96
CBV.dial PRV @21:10:48.97 CTV.dial :air,avoiding: :intake @21:10:48.99 Loading lyfil puck from tube 2 into Collection Clamp @21:10:49.21 SC.reconfigure :tight @21:10:49.25 SC.seek 2 @21:10:52.82 SC.configure :limp @21:10:53.01 Elbow.reconfigure :tight @21:10:53.06 Elbow.seek 2 @21:10:54.99 Forearm.reconfigure :tight @21:10:55.03 Forearm.seek 2 + 700 counts @21:10:56.06 SE.reconfigure :pick @21:10:56.11 SE.seek :beamBreak @21:10:59.08 SE.configure :default @21:10:59.22 Elbow.seek 2 @21:10:59.38 Forearm.seek 2 + 300 counts @21:10:59.55 Forearm.reconfigure :loose @21:10:59.59 Hand.close @21:11:00.25 SE.seek :down @21:11:00.43
Forearm.seek :retract @21:11:01.40 Elbow.seek CC @21:11:02.89 SE.stop @21:11:03.01
Elbow.reconfigure :loose @21:11:03.10 Forearm.reconfigure :tight @21:11:03.14 Forearm.seek CC @21:11:04.36 CC.seek :closed @21:11:08.59 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.71 @21:11:08.60 Hand.open @21:11:09.43 Forearm.reconfigure :loose @21:11:09.47 Forearm.seek :retract @21:11:10.53 CTV.dial CSV,avoiding: :intake @21:11:11.79 CSV.dial CTV @21:11:12.10 PS.seek 2.5ml @21:11:38.28 CS.seek 5.5ml @21:11:50.48 delay 8 seconds @21:11:58.50 PSV.dial :air @21:11:58.95 delay 2 seconds @21:12:00.97 PSV.dial :waste2 @21:12:01.67 PS.seek 0ml @21:12:27.90 CBV.dial between PRV and waste1 @21:12:28.23 CSV.dial :air @21:12:28.69 CS.seek 10ml @21:12:40.85 CSV.dial CTV @21:12:41.40 CS.seek 0ml @21:13:07.41 CBV.dial :waste1 @21:13:07.71 delay 5 seconds @21:13:12.73 CTV.dial :air,avoiding: :intake @21:13:14.03 Storing lyfil puck in Collection Clamp to tube 1 @21:13:14.19 SC.reconfigure :tight @21:13:14.69 SC.seek 1 @21:13:18.31 SC.configure :limp @21:13:18.51 Elbow.reconfigure :tight @21:13:18.55 Elbow.seek 1 @21:13:20.32 Forearm.reconfigure :tight @21:13:20.36 Forearm.seek 1 + 700 counts @21:13:21.43 SE.reconfigure :pick @21:13:21.48 SE.seek :beamBreak @21:13:22.70 SE.configure :default @21:13:22.84 Elbow.reconfigure :limp @21:13:22.89 Forearm.reconfigure :loose @21:13:22.93 Forearm.seek 1 @21:13:23.35 Elbow.hold @21:13:23.41 Elbow.reconfigure :tight @21:13:23.51 SE.seek 28.4 Pucks below Top Plate @21:13:24.02 Forearm.force -20 @21:13:24.06 Forearm.seek 1 + 317 counts @21:13:24.39 Forearm.reconfigure :tight @21:13:24.44 Forearm.seek 1 + 700 counts @21:13:24.64 SE.seek 29.4 Pucks below Top Plate @21:13:25.21 Forearm.reconfigure :loose @21:13:25.25 Forearm.seek :retract @21:13:26.22 Elbow.seek CC @21:13:27.86 Elbow.reconfigure :loose @21:13:27.95 Forearm.seek CC @21:13:28.89 Hand.close @21:13:29.48 CC.seek :open @21:13:33.91 Forearm.seek :retract @21:13:34.98 Elbow.reconfigure :tight @21:13:35.03 Elbow.seek 1 @21:13:36.82 Elbow.reconfigure :limp @21:13:36.87 Forearm.seek 1 @21:13:37.95 Elbow.hold @21:13:38.01 Elbow.reconfigure :tight @21:13:38.05 Hand.open @21:13:38.84 Forearm.force -20 @21:13:38.88 Forearm.seek 1 + 320 counts @21:13:39.24 Forearm.reconfigure :tight @21:13:39.28 Forearm.seek 1 + 700 counts @21:13:39.54 SE.seek :down @21:13:40.34 SE.stop @21:13:40.67
Forearm.reconfigure :loose @21:13:40.71 Forearm.seek :retract @21:13:41.61 PSV.dial :air @21:13:42.33 PS.seek 2.5ml @21:14:08.56 delay 1 second @21:14:09.57 PSV.dial :mixloop @21:14:10.00 delay 1 second @21:14:11.01 PSV.dial :air @21:14:11.43 delay 1 second @21:14:12.45 PSV.dial :mixloop @21:14:12.89 delay 1 second @21:14:13.90 PSV.dial :air @21:14:14.34 delay 1 second @21:14:15.36 PS.seek 0ml @21:14:41.56 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @21:14:41.78 Elbow.seek FlushPuck::Garage @21:14:43.58 Elbow.reconfigure :loose @21:14:43.66 Forearm.seek :garage @21:14:44.77 Hand.close @21:14:45.47 Forearm.seek :retract @21:14:47.51 Elbow.reconfigure :tight @21:14:47.55 Elbow.seek PC @21:14:48.97 Elbow.reconfigure :loose @21:14:49.06 Forearm.reconfigure :tight @21:14:49.10 Forearm.seek PC @21:14:50.93 PC.seek :closed @21:14:54.81 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.43 @21:14:54.87 Hand.open @21:14:55.66 Forearm.reconfigure :loose @21:14:55.70 Forearm.seek :retract @21:14:56.80 PRV.dial :air @21:14:57.26 PSV.dial :mixloop @21:14:57.78 PS.seek 0.3ml @21:15:01.62 PSV.dial PSR @21:15:02.75 Pulling 0.4ml of PSR.air @21:15:02.76 PSR.select :air @21:15:02.78 PS.seek 0.7ml @21:15:07.62 delay 3 seconds @21:15:10.63 PSR.select :air @21:15:10.65 PS.seek 2.5ml @21:15:29.68 delay 3 seconds @21:15:32.69 PSV.dial :mixloop @21:15:33.65 PTV.dial :mixing @21:15:34.33 PBV.dial :waste2 @21:15:35.20 PS.seek 0ml @21:16:01.40 Garaging FlushPuck in Processing Clamp @21:16:01.47 Elbow.reconfigure :tight @21:16:01.51 Elbow.seek PC @21:16:01.73 Elbow.reconfigure :loose @21:16:01.82 Forearm.seek PC @21:16:02.93 Hand.close @21:16:03.48 PC.seek :open @21:16:07.44 Forearm.seek :retract @21:16:08.53 Elbow.reconfigure :tight @21:16:08.57 Elbow.seek :garage @21:16:09.92 Elbow.reconfigure :loose @21:16:10.01 Forearm.reconfigure :tight @21:16:10.05 Forearm.seek :garage @21:16:11.18 Hand.open @21:16:11.91 Forearm.reconfigure :loose @21:16:11.95 Forearm.seek :retract @21:16:13.06 Loading da puck from tube 2 into Processing Clamp @21:16:13.28 SC.reconfigure :tight @21:16:13.32 SC.seek 2 @21:16:16.89 SC.configure :limp @21:16:17.08 Elbow.reconfigure :tight @21:16:17.13 Elbow.seek 2 @21:16:19.09 Forearm.reconfigure :tight @21:16:19.13 Forearm.seek 2 + 700 counts @21:16:20.13 SE.reconfigure :pick @21:16:20.18 SE.seek :beamBreak @21:16:23.27 SE.configure :default @21:16:23.41 Elbow.seek 2 @21:16:23.58 Forearm.seek 2 + 300 counts @21:16:23.74 Forearm.reconfigure :loose @21:16:23.78 Hand.close @21:16:24.37 SE.seek :down @21:16:24.55
Forearm.seek :retract @21:16:25.57 Elbow.seek PC @21:16:27.08 SE.stop @21:16:28.17
Elbow.reconfigure :loose @21:16:28.26 Forearm.reconfigure :tight @21:16:28.30 Forearm.seek PC @21:16:29.67 PC.seek :closed @21:16:33.55 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.38 @21:16:33.56 Hand.open @21:16:34.41 Forearm.reconfigure :loose @21:16:34.46 Forearm.seek :retract @21:16:35.47 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @21:16:35.48 PH.seek 28C,5:00 @21:17:25.20 PH.hold 2:00:00,28C @21:17:25.24 delay 1:30 @21:18:55.28 PSR.select :air @21:18:55.30 PSV.dial PSR @21:18:56.34 Pulling 0.5ml of PSR.wash PSR.select :wash @21:18:56.46 PS.seek 0.5ml @21:19:02.43 delay 5 seconds @21:19:07.44 PSR.select :air @21:19:07.56 PS.seek 2ml @21:19:23.52 PTV.dial :puck @21:19:24.20 PSV.dial PTV @21:19:24.91 PBV.dial :waste1 @21:19:26.76 PS.seek 1.5ml @21:19:32.70 delay 3 seconds @21:19:35.72 PBV.dial between PSV and waste1 @21:19:36.09 PS.seek 0ml @21:19:52.14 delay 5:00 @21:24:52.16 recoverPSRreagent08 @21:24:52.17 PTV.dial :puck @21:24:52.18 PBV.dial :waste1 @21:24:52.54 delay 30 seconds @21:25:22.56 PBV.dial between PSV and waste1 @21:25:22.91 PSR.select :air @21:25:22.93 PSV.dial PSR @21:25:23.65 PS.reconfigure :fast @21:25:23.69 PS.seek 2.5ml @21:25:46.16 PSV.dial PTV @21:25:46.87 delay 5 seconds @21:25:51.88 PSV.dial PSR @21:25:52.60 delay 5 seconds @21:25:57.62 PSV.dial PTV @21:25:58.33 delay 5 seconds @21:26:03.35 PSV.dial :air @21:26:04.06 PS.seek 0ml @21:26:26.63 PTV.dial :PRVmixing,via: PRV @21:26:27.89 PSV.dial :mixloop @21:26:28.32 PRV.dial :ab1 @21:26:29.04 PS.reconfigure :slow @21:26:29.08 PS.seek 0.15ml @21:26:32.69 delay 2 seconds @21:26:34.71 CBV.dial PRV @21:26:34.74
PRV.dial CBV @21:26:35.29 PS.seek 0.34ml @21:26:39.60 delay 2 seconds @21:26:41.61 CBV.dial between PRV and PSV @21:26:41.93 PRV.dial :air @21:26:42.39 PS.seek 0.9ml @21:26:54.16 PRV.dial :waste2 @21:26:54.87 PS.seek 0.5ml @21:27:03.50 addPMLReagent08 @21:27:03.51 PSV.dial :air @21:27:03.95 PS.reconfigure :default @21:27:03.99 PS.seek 2ml @21:27:20.00 PSV.dial :PML @21:27:20.42 PTV.dial :mixing @21:27:21.11 PBV.dial :waste1 @21:27:21.51 PS.seek 1.85ml @21:27:23.81 PBV.dial between PSV and waste1 @21:27:24.18 PS.seek 0ml @21:27:43.78 delay 2 seconds @21:27:45.80 Beginning samplePassC @21:27:45.81 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:27:45.98 Elbow.reconfigure :tight @21:27:46.02 Elbow.seek FlushPuck::Garage @21:27:47.37 Elbow.reconfigure :loose @21:27:47.45 Forearm.seek :garage @21:27:48.59 Hand.close @21:27:49.32 Forearm.seek :retract @21:27:50.38 Elbow.reconfigure :tight @21:27:50.42 Elbow.seek CC @21:27:53.69 Elbow.reconfigure :loose @21:27:53.78 Forearm.reconfigure :tight @21:27:53.82 Forearm.seek CC @21:27:55.04 CC.seek :closed @21:27:59.34 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.84 @21:27:59.41 Hand.open @21:28:00.27 Forearm.reconfigure :loose @21:28:00.31 Forearm.seek :retract @21:28:01.29 PSV.dial PSR @21:28:02.32 Pulling 1.5ml of PSR.flush @21:28:02.33 PSR.select :flush @21:28:02.45 PS.seek 1.5ml @21:28:18.78 delay 5 seconds @21:28:23.80 PSV.dial :waste1 @21:28:24.24 PS.seek 0ml @21:28:40.34 PSV.dial PSR @21:28:40.89 PSR.select :air @21:28:41.00 PS.seek 2.5ml @21:29:07.22 PRV.dial CBV,avoiding: :ab2 @21:29:07.25
CBV.dial PRV @21:29:08.80 PSV.dial :mixloop @21:29:09.80 CTV.dial :air,avoiding: :intake @21:29:09.81 PTV.dial :PRVmixing @21:29:10.51 PS.seek 0ml @21:29:36.68 PSV.dial :air @21:29:37.11 PS.seek 2.5ml @21:30:03.34 PSV.dial :mixloop @21:30:03.80 PS.seek 0ml @21:30:29.96 PSV.dial :air @21:30:30.41 PS.seek 2.5ml @21:30:56.65 PSV.dial :mixloop @21:30:57.10 PS.seek 0ml @21:31:23.31 Garaging FlushPuck in Collection Clamp @21:31:23.38 Elbow.reconfigure :tight @21:31:23.42 Elbow.seek CC @21:31:23.53 Elbow.reconfigure :loose @21:31:23.62 Forearm.seek CC @21:31:24.58 Hand.close @21:31:25.20 CC.seek :open @21:31:29.56 Forearm.seek :retract @21:31:30.56 Elbow.reconfigure :tight @21:31:30.60 Elbow.seek :garage @21:31:33.64 Elbow.reconfigure :loose @21:31:33.73 Forearm.reconfigure :tight @21:31:33.77 Forearm.seek :garage @21:31:34.86 Hand.open @21:31:35.63 Forearm.reconfigure :loose @21:31:35.67 Forearm.seek :retract @21:31:36.68 delayUntil "21:35:45.79" @21:31:36.71 Whole Cell Archive sampling 1000ml @21:31:36.72 Loading wcr puck from tube 2 into Collection Clamp @21:31:36.95 SC.reconfigure :tight @21:31:36.99 SC.seek 2 @21:31:37.09 SC.configure :limp @21:31:37.29 Elbow.reconfigure :tight @21:31:37.34 Elbow.seek 2 @21:31:39.23 Forearm.reconfigure :tight @21:31:39.28 Forearm.seek 2 + 700 counts @21:31:40.32 SE.reconfigure :pick @21:31:40.38 SE.seek :beamBreak @21:31:43.50 SE.configure :default @21:31:43.65 Elbow.seek 2 @21:31:43.82 Forearm.seek 2 + 300 counts @21:31:43.99 Forearm.reconfigure :loose @21:31:44.47 Hand.close @21:31:45.08 SE.seek :down @21:31:45.28 Forearm.seek :retract @21:31:46.34 Elbow.seek CC @21:31:47.92 Elbow.reconfigure :loose @21:31:47.95 SE.stop @21:31:48.07 Forearm.reconfigure :tight @21:31:48.12 Forearm.seek CC @21:31:49.43 CC.seek :closed @21:31:53.65 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.73 @21:31:53.67 Hand.open @21:31:54.50 Forearm.reconfigure :loose @21:31:54.55 Forearm.seek :retract @21:31:55.55 collectSample 1000ml w/bubble point of 35psi @21:31:55.56 Priming 7ml @21:31:55.57 Intake.select :close @21:31:55.58 Exhaust.select :close @21:31:55.59 SS.hold @21:31:55.79 Calibrating pressure sensors @21:31:55.80 CTV.dial :air @21:31:55.81 SSV.dial :spit,avoiding: :exhaust @21:31:55.83 SS.reconfigure :fast @21:31:55.88 SS.seek 5ml @21:31:58.97 SSV.dial CBV @21:31:58.99 CBV.dial SSV @21:32:00.30 delay 3.8 seconds @21:32:04.21 SSV.dial :spit @21:32:05.52 SS.seek 0ml @21:32:08.15 SSV.dial CBV @21:32:09.41 CTV.dial :intake @21:32:10.12 SSV.dial CBV @21:32:10.15 CBV.dial SSV @21:32:10.16 Allowing 40s for 7.0ml of remaining 7.0ml @21:32:10.17 Intake.select :open @21:32:12.32 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:32:12.34 SS.reconfigure :inhaling 2.14ml/s @21:32:12.39 SS.seek 7ml @21:32:19.12 Stroke completed -- waiting for pressure to equalize ... @21:32:19.13 SS.reconfigure :exhale @21:32:19.17 SS.hold @21:32:26.55 Intake.select :close @21:32:26.69 SSV.dial :exhaust @21:32:27.42 Exhaust.select :open @21:32:27.53 SS.seek 0ml @21:32:31.63 Exhaust.select :close @21:32:31.74 Sampling 1000.0ml @21:32:31.75 Intake.select :close @21:32:31.76 Exhaust.select :close @21:32:31.77 SS.reconfigure :default @21:32:31.81 SS.hold @21:32:32.01 CTV.dial :intake @21:32:32.03 SSV.dial CBV @21:32:32.05 CBV.dial SSV @21:32:32.81 Allowing 130s for 25.0ml of remaining 1000.0ml @21:32:32.82 Intake.select :open @21:32:34.97 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:32:34.99 SS.reconfigure :inhaling 2.14ml/s @21:32:35.03 SS.seek 25ml @21:32:40.32 Vacuum too high -- waiting up to 40 seconds @21:32:40.33 SS.reconfigure :exhale @21:32:40.37 SS.hold @21:32:41.62 Intake @12.3psia, vacuum from 6.8 to 9.8psi @21:32:41.64 SS.reconfigure :inhaling 1.93ml/s @21:32:42.17 SS.seek 25ml @21:32:45.22 Vacuum too high -- waiting up to 40 seconds @21:32:45.23 SS.reconfigure :exhale @21:32:45.28 SS.hold @21:32:48.01 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:32:48.03 SS.reconfigure :inhaling 1.74ml/s @21:32:48.07 SS.seek 25ml @21:32:52.19 Vacuum too high -- waiting up to 40 seconds @21:32:52.21 SS.reconfigure :exhale @21:32:52.25 SS.hold @21:32:53.06 Intake @12.4psia, vacuum from 6.9 to 9.9psi @21:32:53.08 SS.reconfigure :inhaling 1.57ml/s @21:32:53.13 SS.seek 25ml @21:32:56.03 Vacuum too high -- waiting up to 40 seconds @21:32:56.05 SS.reconfigure :exhale @21:32:56.09 SS.hold @21:32:58.28 Intake @13.9psia, vacuum from 8.4 to 11.4psi @21:32:58.30 SS.reconfigure :inhaling 1.41ml/s @21:32:58.34 SS.seek 25ml @21:33:03.03 Vacuum too high -- waiting up to 40 seconds @21:33:03.05 SS.reconfigure :exhale @21:33:03.09 SS.hold @21:33:04.82 Intake @12.8psia, vacuum from 7.3 to 10.3psi @21:33:04.84 SS.reconfigure :inhaling 1.27ml/s @21:33:04.88 SS.seek 25ml @21:33:07.48 Stroke completed -- waiting for pressure to equalize ... @21:33:07.49 SS.reconfigure :exhale @21:33:07.53 SS.hold @21:33:09.09 Intake.select :close @21:33:09.23 SSV.dial :exhaust @21:33:09.96 Exhaust.select :open @21:33:10.07 SS.seek 0ml @21:33:18.96 Exhaust.select :close @21:33:19.07 SSV.dial CBV @21:33:19.09 CBV.dial SSV @21:33:19.82 Allowing 130s for 25.0ml of remaining 975.0ml @21:33:19.84 Intake.select :open @21:33:22.00 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:33:22.01 SS.reconfigure :inhaling 1.27ml/s @21:33:22.06 SS.seek 25ml @21:33:27.81 Vacuum too high -- waiting up to 40 seconds @21:33:27.83 SS.reconfigure :exhale @21:33:27.87 SS.hold @21:33:28.32 Intake @12.3psia, vacuum from 6.8 to 9.8psi @21:33:28.34 SS.reconfigure :inhaling 1.14ml/s @21:33:28.38 SS.seek 25ml @21:33:33.79 Vacuum too high -- waiting up to 40 seconds @21:33:33.80 SS.reconfigure :exhale @21:33:33.85 SS.hold @21:33:35.49 Intake @14.2psia, vacuum from 8.7 to 11.7psi @21:33:35.51 SS.reconfigure :inhaling 1.03ml/s @21:33:35.55 SS.seek 25ml @21:33:48.78 Vacuum too high -- waiting up to 40 seconds @21:33:48.80 SS.reconfigure :exhale @21:33:48.84 SS.hold @21:33:50.50 Intake @13.2psia, vacuum from 7.7 to 10.7psi @21:33:50.52 SS.reconfigure :inhaling 0.93ml/s @21:33:50.56 SS.seek 25ml @21:33:54.80 Stroke completed -- waiting for pressure to equalize ... @21:33:54.81 SS.reconfigure :exhale @21:33:54.86 SS.hold @21:33:56.50 Intake.select :close @21:33:56.65 SSV.dial :exhaust @21:33:57.87 Exhaust.select :open @21:33:57.98 SS.seek 0ml @21:34:07.30 Exhaust.select :close @21:34:07.41 SSV.dial CBV @21:34:07.44 CBV.dial SSV @21:34:08.20 Allowing 130s for 25.0ml of remaining 950.0ml @21:34:08.21 Intake.select :open @21:34:10.37 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:34:10.38 SS.reconfigure :inhaling 0.93ml/s @21:34:10.43 SS.seek 25ml @21:34:34.31 Vacuum too high -- waiting up to 40 seconds @21:34:34.32 SS.reconfigure :exhale @21:34:34.37 SS.hold @21:34:35.33 Intake @13.4psia, vacuum from 7.9 to 10.9psi @21:34:35.34 SS.reconfigure :inhaling 0.84ml/s @21:34:35.39 SS.seek 25ml @21:34:41.60 Stroke completed -- waiting for pressure to equalize ... @21:34:41.61 SS.reconfigure :exhale @21:34:41.65 SS.hold @21:34:43.60 Intake.select :close @21:34:43.75 SSV.dial :exhaust @21:34:44.48 Exhaust.select :open @21:34:44.59 SS.seek 0ml @21:34:53.58 Exhaust.select :close @21:34:53.69 SSV.dial CBV @21:34:53.72 CBV.dial SSV @21:34:54.46 Allowing 130s for 25.0ml of remaining 925.0ml @21:34:54.47 Intake.select :open @21:34:56.63 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:34:56.65 SS.reconfigure :inhaling 0.84ml/s @21:34:56.69 SS.seek 25ml @21:35:28.91 Stroke completed -- waiting for pressure to equalize ... @21:35:28.92 SS.reconfigure :exhale @21:35:28.96 SS.hold @21:35:31.96 Intake.select :close @21:35:32.10 SSV.dial :exhaust @21:35:32.83 Exhaust.select :open @21:35:32.94 SS.seek 0ml @21:35:42.00 Exhaust.select :close @21:35:42.11 SSV.dial CBV @21:35:42.14 CBV.dial SSV @21:35:42.90 Allowing 130s for 25.0ml of remaining 900.0ml @21:35:42.91 Intake.select :open @21:35:45.06 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:35:45.08 SS.reconfigure :inhaling 0.84ml/s @21:35:45.13 SS.seek 25ml @21:35:45.80 recoverPRVreagent08 @21:35:45.81 PTV.dial :mixing @21:35:46.52 PBV.dial :waste1 @21:35:46.90 delay 30 seconds @21:36:16.92 PBV.dial between PSV and waste1 @21:36:17.29 PSV.dial :air @21:36:17.35 Stroke completed -- waiting for pressure to equalize ... @21:36:17.36 SS.reconfigure :exhale @21:36:17.40 SS.hold @21:36:17.74 PS.reconfigure :fast @21:36:17.79 PS.seek 2.5ml @21:36:20.88 Intake.select :close @21:36:21.03 SSV.dial :exhaust @21:36:21.75 Exhaust.select :open @21:36:21.86 SS.seek 0ml @21:36:31.06 Exhaust.select :close @21:36:31.17 SSV.dial CBV @21:36:31.20 CBV.dial SSV @21:36:31.94 Allowing 130s for 25.0ml of remaining 875.0ml @21:36:31.95 Intake.select :open @21:36:34.11 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:36:34.12 SS.reconfigure :inhaling 0.84ml/s @21:36:34.17 SS.seek 25ml @21:36:40.52 PSV.dial :mixloop @21:36:40.97 delay 2 seconds @21:36:42.99 PSV.dial :air @21:36:43.44 delay 2 seconds @21:36:45.46 PSV.dial :mixloop @21:36:46.02 delay 2 seconds @21:36:48.03 PSV.dial :air @21:36:48.93 PS.seek 0ml @21:37:02.93 Vacuum too high -- waiting up to 40 seconds @21:37:02.94 SS.reconfigure :exhale @21:37:02.99 SS.hold @21:37:04.27 Intake @13.0psia, vacuum from 7.5 to 10.5psi @21:37:04.29 SS.reconfigure :inhaling 0.76ml/s @21:37:04.34 SS.seek 25ml @21:37:08.10 Stroke completed -- waiting for pressure to equalize ... @21:37:08.11 SS.reconfigure :exhale @21:37:08.15 SS.hold @21:37:09.70 Intake.select :close @21:37:09.85 SSV.dial :exhaust @21:37:10.58 Exhaust.select :open @21:37:10.69 SS.seek 0ml @21:37:11.49 washCycleDA #1 of 2 @21:37:11.50 PSR.select :air @21:37:11.51 PSV.dial PSR @21:37:12.80 Pulling 0.5ml of PSR.wash @21:37:12.81 PSR.select :wash @21:37:12.93 PS.reconfigure :default @21:37:12.97 PS.seek 0.5ml @21:37:18.86 delay 5 seconds @21:37:19.70 Exhaust.select :close @21:37:19.81 SSV.dial CBV @21:37:19.84 CBV.dial SSV @21:37:20.58 Allowing 130s for 25.0ml of remaining 850.0ml @21:37:20.59 Intake.select :open @21:37:22.75 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:37:22.77 SS.reconfigure :inhaling 0.76ml/s @21:37:22.81 SS.seek 25ml @21:37:23.88 PSR.select :air @21:37:24.00 PS.seek 2ml @21:37:39.98 PTV.dial :puck @21:37:40.70 PSV.dial PTV @21:37:41.53 PBV.dial :waste1 @21:37:41.97 PS.seek 1.5ml @21:37:47.92 delay 3 seconds @21:37:50.94 PBV.dial between PSV and waste1 @21:37:51.32 PS.seek 0ml @21:37:58.34 Stroke completed -- waiting for pressure to equalize ... @21:37:58.35 SS.reconfigure :exhale @21:37:58.40 SS.hold @21:38:01.32 Intake.select :close @21:38:01.46 SSV.dial :exhaust @21:38:02.18 Exhaust.select :open @21:38:02.29 SS.seek 0ml @21:38:07.40 delay 2:00 @21:38:11.53 Exhaust.select :close @21:38:11.64 SSV.dial CBV @21:38:11.67 CBV.dial SSV @21:38:12.40 Allowing 130s for 25.0ml of remaining 825.0ml @21:38:12.41 Intake.select :open @21:38:14.57 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:38:14.58 SS.reconfigure :inhaling 0.76ml/s @21:38:14.63 SS.seek 25ml @21:38:49.37 Stroke completed -- waiting for pressure to equalize ... @21:38:49.38 SS.reconfigure :exhale @21:38:49.42 SS.hold @21:38:52.87 Intake.select :close @21:38:53.01 SSV.dial :exhaust @21:38:53.75 Exhaust.select :open @21:38:53.86 SS.seek 0ml @21:39:02.89 Exhaust.select :close @21:39:02.99 SSV.dial CBV @21:39:03.02 CBV.dial SSV @21:39:03.76 Allowing 130s for 25.0ml of remaining 800.0ml @21:39:03.78 Intake.select :open @21:39:05.93 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:39:05.95 SS.reconfigure :inhaling 0.76ml/s @21:39:05.99 SS.seek 25ml @21:39:41.54 Stroke completed -- waiting for pressure to equalize ... @21:39:41.55 SS.reconfigure :exhale @21:39:41.59 SS.hold @21:39:46.21 Intake.select :close @21:39:46.35 SSV.dial :exhaust @21:39:47.07 Exhaust.select :open @21:39:47.19 SS.seek 0ml @21:39:56.17 Exhaust.select :close @21:39:56.27 SSV.dial CBV @21:39:56.30 CBV.dial SSV @21:39:57.06 Allowing 130s for 25.0ml of remaining 775.0ml @21:39:57.07 Intake.select :open @21:39:59.23 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:39:59.25 SS.reconfigure :inhaling 0.76ml/s @21:39:59.29 SS.seek 25ml @21:40:07.42 recoverPSRreagent08 @21:40:07.43 PTV.dial :puck @21:40:07.44 PBV.dial :waste1 @21:40:07.79 delay 30 seconds @21:40:33.58 Vacuum too high -- waiting up to 40 seconds @21:40:33.59 SS.reconfigure :exhale @21:40:33.64 SS.hold @21:40:35.28 Intake @12.8psia, vacuum from 7.3 to 10.3psi @21:40:35.30 SS.reconfigure :inhaling 0.69ml/s @21:40:35.35 SS.seek 25ml @21:40:37.18 Stroke completed -- waiting for pressure to equalize ... @21:40:37.19 SS.reconfigure :exhale @21:40:37.23 SS.hold @21:40:37.81 PBV.dial between PSV and waste1 @21:40:38.17 PSR.select :air @21:40:38.19 PSV.dial PSR @21:40:38.89 PS.reconfigure :fast @21:40:38.94 PS.seek 2.5ml @21:40:40.14 Intake.select :close @21:40:40.29 SSV.dial :exhaust @21:40:41.01 Exhaust.select :open @21:40:41.12 SS.seek 0ml @21:40:50.13 Exhaust.select :close @21:40:50.24 SSV.dial CBV @21:40:50.27 CBV.dial SSV @21:40:51.01 Allowing 130s for 25.0ml of remaining 750.0ml @21:40:51.02 Intake.select :open @21:40:53.18 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:40:53.19 SS.reconfigure :inhaling 0.69ml/s @21:40:53.24 SS.seek 25ml @21:41:01.73 PSV.dial PTV @21:41:02.50 delay 5 seconds @21:41:07.52 PSV.dial PSR @21:41:08.23 delay 5 seconds @21:41:13.25 PSV.dial PTV @21:41:13.96 delay 5 seconds @21:41:18.98 PSV.dial :air @21:41:19.69 PS.seek 0ml @21:41:32.16 Stroke completed -- waiting for pressure to equalize ... @21:41:32.17 SS.reconfigure :exhale @21:41:32.22 SS.hold @21:41:36.28 Intake.select :close @21:41:36.42 SSV.dial :exhaust @21:41:37.15 Exhaust.select :open @21:41:37.27 SS.seek 0ml @21:41:42.13 washCycleDA #2 of 2 @21:41:42.14 PSR.select :air @21:41:42.16 PSV.dial PSR @21:41:43.59 Pulling 0.5ml of PSR.wash @21:41:43.60 PSR.select :wash @21:41:43.72 PS.reconfigure :default @21:41:43.76 PS.seek 0.5ml @21:41:46.30 Exhaust.select :close @21:41:46.41 SSV.dial CBV @21:41:46.44 CBV.dial SSV @21:41:47.20 Allowing 130s for 25.0ml of remaining 725.0ml @21:41:47.22 Intake.select :open @21:41:49.37 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:41:49.39 SS.reconfigure :inhaling 0.69ml/s @21:41:49.43 SS.seek 25ml @21:41:49.83 delay 5 seconds @21:41:54.84 PSR.select :air @21:41:54.95 PS.seek 2ml @21:42:11.05 PTV.dial :puck @21:42:11.07 PSV.dial PTV @21:42:11.79 PBV.dial :waste1 @21:42:12.21 PS.seek 1.5ml @21:42:18.10 delay 3 seconds @21:42:21.12 PBV.dial between PSV and waste1 @21:42:21.49 PS.seek 0ml @21:42:28.44 Stroke completed -- waiting for pressure to equalize ... @21:42:28.45 SS.reconfigure :exhale @21:42:28.50 SS.hold @21:42:34.04 Intake.select :close @21:42:34.18 SSV.dial :exhaust @21:42:34.91 Exhaust.select :open @21:42:35.02 SS.seek 0ml @21:42:37.51 delay 2:00 @21:42:44.05 Exhaust.select :close @21:42:44.16 SSV.dial CBV @21:42:44.19 CBV.dial SSV @21:42:44.93 Allowing 130s for 25.0ml of remaining 700.0ml @21:42:44.94 Intake.select :open @21:42:47.10 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:42:47.11 SS.reconfigure :inhaling 0.69ml/s @21:42:47.16 SS.seek 25ml @21:43:14.08 Vacuum too high -- waiting up to 40 seconds @21:43:14.09 SS.reconfigure :exhale @21:43:14.13 SS.hold @21:43:16.18 Intake @13.7psia, vacuum from 8.2 to 11.2psi @21:43:16.20 SS.reconfigure :inhaling 0.61ml/s @21:43:16.24 SS.seek 25ml @21:43:29.47 Stroke completed -- waiting for pressure to equalize ... @21:43:29.48 SS.reconfigure :exhale @21:43:29.53 SS.hold @21:43:33.70 Intake.select :close @21:43:33.84 SSV.dial :exhaust @21:43:34.57 Exhaust.select :open @21:43:34.68 SS.seek 0ml @21:43:44.12 Exhaust.select :close @21:43:44.23 SSV.dial CBV @21:43:44.25 CBV.dial SSV @21:43:44.99 Allowing 130s for 25.0ml of remaining 675.0ml @21:43:45.00 Intake.select :open @21:43:47.16 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:43:47.17 SS.reconfigure :inhaling 0.61ml/s @21:43:47.22 SS.seek 25ml @21:44:29.77 Stroke completed -- waiting for pressure to equalize ... @21:44:29.78 SS.reconfigure :exhale @21:44:29.83 SS.hold @21:44:35.36 Intake.select :close @21:44:35.51 SSV.dial :exhaust @21:44:36.22 Exhaust.select :open @21:44:36.33 SS.seek 0ml @21:44:37.53 recoverPSRreagent08 @21:44:37.54 PTV.dial :puck @21:44:37.55 PBV.dial :waste1 @21:44:37.90 delay 30 seconds @21:44:45.39 Exhaust.select :close @21:44:45.50 SSV.dial CBV @21:44:45.54 CBV.dial SSV @21:44:46.25 Allowing 130s for 25.0ml of remaining 650.0ml @21:44:46.27 Intake.select :open @21:44:48.42 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:44:48.44 SS.reconfigure :inhaling 0.61ml/s @21:44:48.48 SS.seek 25ml @21:45:07.92 PBV.dial between PSV and waste1 @21:45:08.27 PSR.select :air @21:45:08.29 PSV.dial PSR @21:45:09.01 PS.reconfigure :fast @21:45:09.05 PS.seek 2.5ml @21:45:28.51 Vacuum too high -- waiting up to 40 seconds @21:45:28.52 SS.reconfigure :exhale @21:45:28.56 SS.hold @21:45:31.57 Intake @13.8psia, vacuum from 8.3 to 11.3psi @21:45:31.59 SS.reconfigure :inhaling 0.56ml/s @21:45:31.63 SS.seek 25ml @21:45:31.70 PSV.dial PTV @21:45:32.55 delay 5 seconds @21:45:36.83 Stroke completed -- waiting for pressure to equalize ... @21:45:36.84 SS.reconfigure :exhale @21:45:36.88 SS.hold @21:45:37.56 PSV.dial PSR @21:45:38.27 delay 5 seconds @21:45:40.35 Intake.select :close @21:45:40.50 SSV.dial :exhaust @21:45:41.23 Exhaust.select :open @21:45:41.34 SS.seek 0ml @21:45:43.28 PSV.dial PTV @21:45:44.00 delay 5 seconds @21:45:49.02 PSV.dial :air @21:45:49.74 PS.seek 0ml @21:45:50.75 Exhaust.select :close @21:45:50.86 SSV.dial CBV @21:45:50.88 CBV.dial SSV @21:45:51.63 Allowing 130s for 25.0ml of remaining 625.0ml @21:45:51.64 Intake.select :open @21:45:53.79 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:45:53.81 SS.reconfigure :inhaling 0.56ml/s @21:45:53.85 SS.seek 25ml @21:46:12.32 getPMLReagent08 @21:46:12.33 PRV.dial :air @21:46:12.75 delay 5 seconds @21:46:17.77 PTV.dial :PRVmixing @21:46:19.02 delay 5 seconds @21:46:24.04 PRV.dial :ab2 @21:46:24.48 PTV.dial :PRVmixing @21:46:24.50 PSV.dial :mixloop @21:46:24.94 PS.reconfigure :default @21:46:24.99 PS.seek 0.3ml @21:46:28.91 delay 2 seconds @21:46:30.92 PRV.dial :air @21:46:31.42 PS.seek 0.55ml @21:46:34.75 addPMLReagent08 @21:46:34.76 PSV.dial :air @21:46:35.21 PS.seek 2ml @21:46:40.98 Stroke completed -- waiting for pressure to equalize ... @21:46:40.99 SS.reconfigure :exhale @21:46:41.04 SS.hold @21:46:47.44 Intake.select :close @21:46:47.58 SSV.dial :exhaust @21:46:48.31 Exhaust.select :open @21:46:48.42 SS.seek 0ml @21:46:50.81 PSV.dial :PML @21:46:51.25 PTV.dial :mixing @21:46:52.01 PBV.dial :waste1 @21:46:52.46 PS.seek 1.85ml @21:46:54.81 PBV.dial between PSV and waste1 @21:46:55.18 PS.seek 0ml @21:46:57.75 Exhaust.select :close @21:46:57.86 SSV.dial CBV @21:46:57.89 CBV.dial SSV @21:46:58.64 Allowing 130s for 25.0ml of remaining 600.0ml @21:46:58.65 Intake.select :open @21:47:00.81 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:47:00.82 SS.reconfigure :inhaling 0.56ml/s @21:47:00.87 SS.seek 25ml @21:47:14.72 delay 2 seconds @21:47:16.74 delay 8:00 @21:47:38.65 Vacuum too high -- waiting up to 40 seconds @21:47:38.66 SS.reconfigure :exhale @21:47:38.70 SS.hold @21:47:42.14 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:47:42.16 SS.reconfigure :inhaling 0.50ml/s @21:47:42.21 SS.seek 25ml @21:47:53.06 Stroke completed -- waiting for pressure to equalize ... @21:47:53.07 SS.reconfigure :exhale @21:47:53.11 SS.hold @21:47:58.11 Intake.select :close @21:47:58.25 SSV.dial :exhaust @21:47:58.98 Exhaust.select :open @21:47:59.09 SS.seek 0ml @21:48:08.46 Exhaust.select :close @21:48:08.57 SSV.dial CBV @21:48:08.61 CBV.dial SSV @21:48:09.33 Allowing 130s for 25.0ml of remaining 575.0ml @21:48:09.34 Intake.select :open @21:48:11.49 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:48:11.51 SS.reconfigure :inhaling 0.50ml/s @21:48:11.55 SS.seek 25ml @21:49:04.32 Stroke completed -- waiting for pressure to equalize ... @21:49:04.33 SS.reconfigure :exhale @21:49:04.37 SS.hold @21:49:11.74 Intake.select :close @21:49:11.89 SSV.dial :exhaust @21:49:12.62 Exhaust.select :open @21:49:12.73 SS.seek 0ml @21:49:22.18 Exhaust.select :close @21:49:22.29 SSV.dial CBV @21:49:22.32 CBV.dial SSV @21:49:23.04 Allowing 130s for 25.0ml of remaining 550.0ml @21:49:23.05 Intake.select :open @21:49:25.21 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:49:25.23 SS.reconfigure :inhaling 0.50ml/s @21:49:25.27 SS.seek 25ml @21:50:12.84 Vacuum too high -- waiting up to 40 seconds @21:50:12.86 SS.reconfigure :exhale @21:50:12.90 SS.hold @21:50:17.07 Intake @14.1psia, vacuum from 8.6 to 11.6psi @21:50:17.09 SS.reconfigure :inhaling 0.46ml/s @21:50:17.13 SS.seek 25ml @21:50:23.94 Stroke completed -- waiting for pressure to equalize ... @21:50:23.95 SS.reconfigure :exhale @21:50:24.00 SS.hold @21:50:29.63 Intake.select :close @21:50:29.77 SSV.dial :exhaust @21:50:30.49 Exhaust.select :open @21:50:31.11 SS.seek 0ml @21:50:40.44 Exhaust.select :close @21:50:40.55 SSV.dial CBV @21:50:40.59 CBV.dial SSV @21:50:41.30 Allowing 130s for 25.0ml of remaining 525.0ml @21:50:41.32 Intake.select :open @21:50:43.47 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:50:43.49 SS.reconfigure :inhaling 0.46ml/s @21:50:43.54 SS.seek 25ml @21:51:43.36 Stroke completed -- waiting for pressure to equalize ... @21:51:43.37 SS.reconfigure :exhale @21:51:43.42 SS.hold @21:51:51.87 Intake.select :close @21:51:52.02 SSV.dial :exhaust @21:51:52.75 Exhaust.select :open @21:51:52.86 SS.seek 0ml @21:52:01.89 Exhaust.select :close @21:52:02.00 SSV.dial CBV @21:52:02.03 CBV.dial SSV @21:52:02.77 Allowing 130s for 25.0ml of remaining 500.0ml @21:52:02.78 Intake.select :open @21:52:04.94 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:52:04.95 SS.reconfigure :inhaling 0.46ml/s @21:52:05.00 SS.seek 25ml @21:52:51.62 Vacuum too high -- waiting up to 40 seconds @21:52:51.63 SS.reconfigure :exhale @21:52:51.67 SS.hold @21:52:57.19 Intake @14.7psia, vacuum from 9.2 to 12.2psi @21:52:57.21 SS.reconfigure :inhaling 0.41ml/s @21:52:57.25 SS.seek 25ml @21:53:13.19 Stroke completed -- waiting for pressure to equalize ... @21:53:13.20 SS.reconfigure :exhale @21:53:13.24 SS.hold @21:53:20.72 Intake.select :close @21:53:20.86 SSV.dial :exhaust @21:53:21.58 Exhaust.select :open @21:53:21.69 SS.seek 0ml @21:53:30.72 Exhaust.select :close @21:53:30.83 SSV.dial CBV @21:53:30.85 CBV.dial SSV @21:53:31.60 Allowing 130s for 25.0ml of remaining 475.0ml @21:53:31.61 Intake.select :open @21:53:33.76 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:53:33.78 SS.reconfigure :inhaling 0.41ml/s @21:53:33.82 SS.seek 25ml @21:54:40.39 Vacuum too high -- waiting up to 40 seconds @21:54:40.41 SS.reconfigure :exhale @21:54:40.45 SS.hold @21:54:45.96 Intake @14.0psia, vacuum from 8.5 to 11.5psi @21:54:45.98 SS.reconfigure :inhaling 0.37ml/s @21:54:46.02 SS.seek 25ml @21:54:49.81 Stroke completed -- waiting for pressure to equalize ... @21:54:50.33 SS.reconfigure :exhale @21:54:50.37 SS.hold @21:54:55.84 Intake.select :close @21:54:55.98 SSV.dial :exhaust @21:54:56.72 Exhaust.select :open @21:54:56.84 SS.seek 0ml @21:55:05.86 Exhaust.select :close @21:55:05.96 SSV.dial CBV @21:55:05.99 CBV.dial SSV @21:55:06.73 Allowing 130s for 25.0ml of remaining 450.0ml @21:55:06.75 Intake.select :open @21:55:08.90 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:55:08.92 SS.reconfigure :inhaling 0.37ml/s @21:55:08.96 SS.seek 25ml @21:55:17.16 recoverPRVreagent08 @21:55:17.17 PTV.dial :mixing @21:55:17.18 PBV.dial :waste1 @21:55:17.53 delay 30 seconds @21:55:47.56 PBV.dial between PSV and waste1 @21:55:47.91 PSV.dial :air @21:55:48.37 PS.reconfigure :fast @21:55:48.41 PS.seek 2.5ml @21:56:10.95 PSV.dial :mixloop @21:56:11.41 delay 2 seconds @21:56:13.42 PSV.dial :air @21:56:13.88 delay 2 seconds @21:56:15.89 PSV.dial :mixloop @21:56:16.35 delay 2 seconds @21:56:18.37 PSV.dial :air @21:56:18.82 PS.seek 0ml @21:56:28.24 Stroke completed -- waiting for pressure to equalize ... @21:56:28.25 SS.reconfigure :exhale @21:56:28.30 SS.hold @21:56:39.35 Intake.select :close @21:56:39.50 SSV.dial :exhaust @21:56:40.23 Exhaust.select :open @21:56:40.34 SS.seek 0ml @21:56:41.42 washCycleDA #1 of 3 @21:56:41.43 PSR.select :air @21:56:41.45 PSV.dial PSR @21:56:42.75 Pulling 0.5ml of PSR.wash @21:56:42.76 PSR.select :wash @21:56:42.88 PS.reconfigure :default @21:56:42.92 PS.seek 0.5ml @21:56:48.86 delay 5 seconds @21:56:49.66 Exhaust.select :close @21:56:49.77 SSV.dial CBV @21:56:49.80 CBV.dial SSV @21:56:51.18 Allowing 130s for 25.0ml of remaining 425.0ml @21:56:51.19 Intake.select :open @21:56:53.35 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:56:53.37 SS.reconfigure :inhaling 0.37ml/s @21:56:53.41 SS.seek 25ml @21:56:53.87 PSR.select :air @21:56:53.99 PS.seek 2ml @21:57:09.98 PTV.dial :puck @21:57:10.70 PSV.dial PTV @21:57:11.40 PBV.dial :waste1 @21:57:11.82 PS.seek 1.5ml @21:57:17.70 delay 3 seconds @21:57:20.72 PBV.dial between PSV and waste1 @21:57:21.08 PS.seek 0ml @21:57:37.17 delay 2:00 @21:58:05.92 Vacuum too high -- waiting up to 40 seconds @21:58:05.93 SS.reconfigure :exhale @21:58:05.98 SS.hold @21:58:13.45 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:58:13.47 SS.reconfigure :inhaling 0.33ml/s @21:58:13.51 SS.seek 25ml @21:58:20.75 Stroke completed -- waiting for pressure to equalize ... @21:58:20.77 SS.reconfigure :exhale @21:58:20.81 SS.hold @21:58:28.18 Intake.select :close @21:58:28.32 SSV.dial :exhaust @21:58:29.05 Exhaust.select :open @21:58:29.16 SS.seek 0ml @21:58:38.61 Exhaust.select :close @21:58:38.72 SSV.dial CBV @21:58:38.75 CBV.dial SSV @21:58:39.49 Allowing 130s for 25.0ml of remaining 400.0ml @21:58:39.50 Intake.select :open @21:58:41.67 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:58:41.69 SS.reconfigure :inhaling 0.33ml/s @21:58:41.73 SS.seek 25ml @21:59:37.19 recoverPSRreagent08 @21:59:37.20 PTV.dial :puck @21:59:37.21 PBV.dial :waste1 @21:59:37.56 delay 30 seconds @22:00:07.58 PBV.dial between PSV and waste1 @22:00:07.93 PSR.select :air @22:00:07.95 PSV.dial PSR @22:00:08.67 PS.reconfigure :fast @22:00:08.71 PS.seek 2.5ml @22:00:12.06 Stroke completed -- waiting for pressure to equalize ... @22:00:12.08 SS.reconfigure :exhale @22:00:12.12 SS.hold @22:00:26.38 Intake.select :close @22:00:26.52 SSV.dial :exhaust @22:00:27.25 Exhaust.select :open @22:00:27.36 SS.seek 0ml @22:00:31.38 PSV.dial PTV @22:00:32.10 delay 5 seconds @22:00:36.76 Exhaust.select :close @22:00:36.87 SSV.dial CBV @22:00:36.89 CBV.dial SSV @22:00:37.12 PSV.dial PSR @22:00:37.64 Allowing 130s for 25.0ml of remaining 375.0ml @22:00:37.66 Intake.select :open @22:00:37.84 delay 5 seconds @22:00:39.81 Intake @15.2psia, vacuum from 9.7 to 12.7psi @22:00:39.83 SS.reconfigure :inhaling 0.33ml/s @22:00:39.88 SS.seek 25ml @22:00:42.85 PSV.dial PTV @22:00:43.68 delay 5 seconds @22:00:48.70 PSV.dial :air @22:00:49.44 PS.seek 0ml @22:01:11.92 washCycleDA #2 of 3 PSR.select :air @22:01:11.94 PSV.dial PSR @22:01:13.23 Pulling 0.5ml of PSR.wash @22:01:13.24 PSR.select :wash @22:01:13.35 PS.reconfigure :default @22:01:13.40 PS.seek 0.5ml @22:01:19.35 delay 5 seconds @22:01:24.37 PSR.select :air @22:01:24.48 PS.seek 2ml @22:01:40.54 PTV.dial :puck @22:01:40.55 PSV.dial PTV @22:01:41.36 PBV.dial :waste1 @22:01:41.78 PS.seek 1.5ml @22:01:43.23 Vacuum too high -- waiting up to 40 seconds @22:01:43.24 SS.reconfigure :exhale @22:01:43.28 SS.hold @22:01:47.66 delay 3 seconds @22:01:49.69 Intake @14.4psia, vacuum from 8.9 to 11.9psi @22:01:49.71 SS.reconfigure :inhaling 0.30ml/s @22:01:49.75 SS.seek 25ml @22:01:50.68 PBV.dial between PSV and waste1 @22:01:51.05 PS.seek 0ml @22:02:07.07 delay 2:00 @22:02:20.18 Stroke completed -- waiting for pressure to equalize ... @22:02:20.20 SS.reconfigure :exhale @22:02:20.24 SS.hold @22:02:34.61 Intake.select :close @22:02:34.75 SSV.dial :exhaust @22:02:35.48 Exhaust.select :open @22:02:35.59 SS.seek 0ml @22:02:44.67 Exhaust.select :close @22:02:44.78 SSV.dial CBV @22:02:44.80 CBV.dial SSV @22:02:45.55 Allowing 130s for 25.0ml of remaining 350.0ml @22:02:45.56 Intake.select :open @22:02:47.71 Intake @15.2psia, vacuum from 9.7 to 12.7psi @22:02:47.73 SS.reconfigure :inhaling 0.30ml/s @22:02:47.77 SS.seek 25ml @22:04:07.09 recoverPSRreagent08 @22:04:07.10 PTV.dial :puck @22:04:07.11 PBV.dial :waste1 @22:04:07.47 delay 30 seconds @22:04:13.35 Vacuum too high -- waiting up to 40 seconds @22:04:13.36 SS.reconfigure :exhale @22:04:13.40 SS.hold @22:04:23.17 Intake @14.9psia, vacuum from 9.4 to 12.4psi @22:04:23.18 SS.reconfigure :inhaling 0.27ml/s @22:04:23.23 SS.seek 25ml @22:04:37.49 PBV.dial between PSV and waste1 @22:04:37.85 PSR.select :air @22:04:37.86 PSV.dial PSR @22:04:38.57 PS.reconfigure :fast @22:04:38.61 PS.seek 2.5ml @22:04:40.95 Stroke completed -- waiting for pressure to equalize ... @22:04:40.96 SS.reconfigure :exhale @22:04:41.00 SS.hold @22:04:55.29 Intake.select :close @22:04:55.43 SSV.dial :exhaust @22:04:56.16 Exhaust.select :open @22:04:56.81 SS.seek 0ml @22:05:01.28 PSV.dial PTV @22:05:02.00 delay 5 seconds @22:05:06.21 Exhaust.select :close @22:05:06.32 SSV.dial CBV @22:05:06.36 CBV.dial SSV @22:05:07.02 PSV.dial PSR @22:05:07.10 Allowing 130s for 25.0ml of remaining 325.0ml @22:05:07.12 Intake.select :open @22:05:07.74 delay 5 seconds @22:05:09.27 Intake @15.2psia, vacuum from 9.7 to 12.7psi @22:05:09.29 SS.reconfigure :inhaling 0.27ml/s @22:05:09.34 SS.seek 25ml @22:05:12.75 PSV.dial PTV @22:05:13.47 delay 5 seconds @22:05:18.49 PSV.dial :air @22:05:19.19 PS.seek 0ml @22:05:41.59 washCycleDA #3 of 3 @22:05:41.60 PSR.select :air @22:05:41.62 PSV.dial PSR @22:05:42.91 Pulling 0.5ml of PSR.wash @22:05:42.92 PSR.select :wash @22:05:43.04 PS.reconfigure :default @22:05:43.08 PS.seek 0.5ml @22:05:49.03 delay 5 seconds @22:05:54.05 PSR.select :air @22:05:54.16 PS.seek 2ml @22:06:10.25 PTV.dial :puck @22:06:10.26 PSV.dial PTV @22:06:11.08 PBV.dial :waste1 @22:06:11.50 PS.seek 1.5ml @22:06:17.34 delay 3 seconds @22:06:20.36 PBV.dial between PSV and waste1 @22:06:20.73 PS.seek 0ml @22:06:36.75 delay 2:00 @22:06:49.59 Vacuum too high -- waiting up to 40 seconds @22:06:49.60 SS.reconfigure :exhale @22:06:49.65 SS.hold @22:07:02.33 Intake @15.3psia, vacuum from 9.8 to 12.8psi @22:07:02.35 SS.reconfigure :inhaling 0.24ml/s @22:07:02.39 SS.seek 25ml @22:07:14.58 Stroke completed -- waiting for pressure to equalize ... @22:07:14.59 SS.reconfigure :exhale @22:07:14.63 SS.hold @22:07:17.13 SS.reconfigure :default @22:07:17.17 SS.hold @22:07:17.28 Intake.select :close @22:07:17.43 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 700.0ml @22:07:17.44 SSV.dial :exhaust @22:07:18.18 Exhaust.select :open @22:07:18.29 SS.reconfigure :exhale @22:07:18.33 SS.seek 0ml @22:07:27.71 Exhaust.select :close @22:07:27.81 Sampled 700.0ml @22:07:27.83 Drying puck before fixing for 20:00 @22:07:27.84 CBV.dial :waste2b @22:07:28.56 delay 1:00 @22:08:28.59 CSV.dial :air @22:08:29.00 CS.reconfigure :fast @22:08:29.05 CS.seek 10ml @22:08:36.77 recoverPSRreagent08 @22:08:36.78 PTV.dial :puck @22:08:36.79 PBV.dial :waste1 @22:08:37.15 delay 30 seconds @22:08:51.33 Pumping wcr puck from tube 2 up to 35psi... @22:08:51.34 CBV.dial SSV @22:08:52.61 CBV.dial between CSV and waste2b @22:08:53.52 CSV.dial between air and CTV @22:08:53.85 CS.seek 7.535ml @22:09:00.08 CTV.dial CSV,avoiding: :air @22:09:00.12 CSV.dial CTV @22:09:00.84 CS.reconfigure :pressurize @22:09:01.40 CS.seek 0ml @22:09:07.17 PBV.dial between PSV and waste1 @22:09:07.53 PSR.select :air @22:09:07.54 PSV.dial PSR @22:09:08.25 PS.reconfigure :fast @22:09:08.30 PS.seek 2.5ml @22:09:16.37 CBV.dial :waste2b @22:09:16.70 CS.reconfigure :default @22:09:16.75 CS.seek 0ml @22:09:22.01 delay 1:00 @22:09:30.84 PSV.dial PTV @22:09:31.56 delay 5 seconds @22:09:36.58 PSV.dial PSR @22:09:37.29 delay 5 seconds @22:09:42.31 PSV.dial PTV @22:09:43.02 delay 5 seconds @22:09:48.04 PSV.dial :air @22:09:48.75 PS.seek 0ml @22:10:11.05 Gate.power :camera,:ON @22:10:11.07 Mixing DA Substrates @22:10:11.08 PSR.select :air @22:10:11.09 PSV.dial PSR @22:10:12.77 Pulling 0.25ml of PSR.sub1 @22:10:12.82 PSR.select :sub1 @22:10:13.13 PS.reconfigure :default @22:10:13.28 PS.seek 0.25ml @22:10:16.68 delay 5 seconds @22:10:21.75 Pulling 0.25ml of PSR.sub2 @22:10:21.76 PSR.select :sub2 @22:10:21.88 PS.seek 0.5ml @22:10:22.03 CTV.dial :air,avoiding: :intake @22:10:23.30 Fix #1 of 2 (with RNAlater) for 10:00 @22:10:23.31 CSV.dial CSR @22:10:23.82 Pulling 2ml of CSR.RNAlater @22:10:23.83 CSR.select :RNAlater @22:10:23.94 CS.seek 2ml @22:10:25.28 delay 5 seconds @22:10:29.89 delay 5 seconds @22:10:30.29 PSR.select :air @22:10:30.40 PS.seek 2ml @22:10:34.90 CSR.select :air @22:10:35.02 CS.reconfigure :fast @22:10:35.07 CS.seek 10ml @22:10:46.43 PTV.dial :puck @22:10:46.44 PSV.dial PTV @22:10:47.18 PBV.dial :waste1 @22:10:47.60 PS.seek 1.5ml @22:10:53.26 Pumping wcr puck from tube 2 up to 35psi... @22:10:53.27 CBV.dial SSV @22:10:53.43 delay 3 seconds @22:10:54.54 CBV.dial between CSV and waste2b @22:10:55.46 CSV.dial between air and CTV @22:10:56.05 CS.seek 7.295ml @22:10:56.44 PBV.dial between PSV and waste1 @22:10:56.81 PS.seek 0ml @22:11:02.79 CTV.dial CSV,avoiding: :air @22:11:02.82 CSV.dial CTV @22:11:04.14 CS.reconfigure :pressurize @22:11:04.19 CS.seek 0ml @22:11:12.90 delay 10 seconds @22:11:20.90 delay 10:00 @22:11:22.92 recoverPSRreagent08 @22:11:22.93 PTV.dial :puck @22:11:22.94 PBV.dial :waste1 @22:11:23.29 delay 30 seconds @22:11:53.31 PBV.dial between PSV and waste1 @22:11:53.66 PSR.select :air @22:11:53.68 PSV.dial PSR @22:11:54.40 PS.reconfigure :fast @22:11:54.44 PS.seek 2.5ml @22:12:17.02 PSV.dial PTV @22:12:17.74 delay 5 seconds @22:12:22.76 PSV.dial PSR @22:12:23.47 delay 5 seconds @22:12:28.49 PSV.dial PTV @22:12:29.21 delay 5 seconds @22:12:34.23 PSV.dial :air @22:12:34.95 PS.seek 0ml @22:12:57.43 Additional air clear @22:12:57.44 PSR.select :air @22:12:57.45 PSV.dial PSR @22:12:58.74 Pulling 2ml of PSR.air @22:12:58.75 PSR.select :air @22:12:58.78 PS.reconfigure :default @22:12:58.82 PS.seek 2ml @22:13:19.92 delay 5 seconds @22:13:24.94 PTV.dial :puck @22:13:24.95 PSV.dial PTV @22:13:25.67 PBV.dial :waste1 @22:13:26.09 PS.seek 1.5ml @22:13:31.95 delay 3 seconds @22:13:34.97 PBV.dial between PSV and waste1 @22:13:35.33 delay 1 second @22:13:36.35 PSV.dial :waste1 @22:13:37.36 PS.seek 0ml @22:13:53.44 Processing Puck Heater at 28C before imaging @22:13:53.45 PH.coast @22:13:54.07 Imaging da puck in Processing Clamp while storing it to tube 1 @22:13:54.24 SC.reconfigure :tight @22:13:54.28 SC.seek 1 @22:13:57.95 SC.configure :limp @22:13:58.15 Elbow.reconfigure :tight @22:13:58.20 Elbow.seek 1 @22:13:59.88 Forearm.reconfigure :tight @22:13:59.92 Forearm.seek 1 + 700 counts @22:14:01.01 SE.reconfigure :pick @22:14:01.06 SE.seek :beamBreak @22:14:02.21 SE.configure :default @22:14:02.35 Elbow.reconfigure :limp @22:14:02.40 Forearm.reconfigure :loose @22:14:02.44 Forearm.seek 1 @22:14:02.87 Elbow.hold @22:14:02.94 Elbow.reconfigure :tight @22:14:03.04 SE.seek 29.4 Pucks below Top Plate @22:14:03.53 Forearm.force -20 @22:14:03.57 Forearm.seek 1 + 314 counts @22:14:03.88 Forearm.reconfigure :tight @22:14:03.93 Forearm.seek 1 + 700 counts @22:14:04.24 SE.seek 30.4 Pucks below Top Plate @22:14:04.79 Forearm.reconfigure :loose @22:14:04.83 Forearm.seek :retract @22:14:05.83 Elbow.seek PC @22:14:08.53 Elbow.reconfigure :loose @22:14:08.62 Forearm.seek PC @22:14:09.68 Hand.close @22:14:10.24 PC.seek :open @22:14:14.24 Forearm.seek :retract @22:14:15.27 Elbow.reconfigure :tight @22:14:15.31 Elbow.seek 1 @22:14:17.86 Forearm.reconfigure :tight @22:14:17.90 Forearm.seek 1 + 700 counts @22:14:18.90 Hand.open @22:14:19.67 SE.seek 30.05 Pucks below Top Plate @22:14:19.97 SE.reconfigure :seal @22:14:20.02 SE.seek 28.94 Pucks below Top Plate @22:14:20.56 Lights, camera, ACTION! @22:14:20.60 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22nov1122h1000ml.tif @22:14:38.52 SX MX5/16 (USB:15): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 14.427 seconds /var/log/esp/da22nov1122h1000ml.tif: TIFF Upload Complete @22:14:38.55 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22nov1122h1000ml15s.tif @22:14:40.06 Waiting >20 seconds for da to relinquish FlushPuck @22:14:54.30 SX MX5/16 (USB:15): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22nov1122h1000ml15s.tif: TIFF Upload Complete @22:14:54.33 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da22nov1122h1000ml25s.tif @22:15:20.08 SX MX5/16 (USB:15): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da22nov1122h1000ml25s.tif: TIFF Upload Complete @22:15:20.10 Gate.power :camera,:OFF @22:15:20.11 DAfans.email "14.427 sec auto-exposure",Subject: "da22nov1122h1000ml images!" @22:15:20.42 Elbow.seek 1 @22:15:20.59 Forearm.seek 1 + 700 counts @22:15:20.67 SE.reconfigure :gently @22:15:20.71 SE.seek 29.66 Pucks below Top Plate @22:15:21.38 Elbow.reconfigure :limp @22:15:21.43 Forearm.reconfigure :loose @22:15:21.49 Forearm.seek 1 @22:15:21.93 Elbow.hold @22:15:22.00 Elbow.reconfigure :tight @22:15:22.06 SE.reconfigure :default @22:15:22.10 SE.seek 29.84 Pucks below Top Plate @22:15:23.40 Forearm.force -20 @22:15:23.44 Forearm.seek 1 + 309 counts @22:15:23.78 Forearm.reconfigure :tight @22:15:23.83 Forearm.seek 1 + 700 counts @22:15:24.87 SE.seek :down @22:15:25.63 SE.stop @22:15:26.03 Forearm.reconfigure :loose @22:15:26.08 Forearm.seek :retract @22:15:27.11 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:15:27.38 Elbow.seek FlushPuck::Garage @22:15:29.49 Elbow.reconfigure :loose @22:15:29.69 Forearm.seek :garage @22:15:30.82 Hand.close @22:15:31.77 Forearm.seek :retract @22:15:32.89 Elbow.reconfigure :tight @22:15:32.97 Elbow.seek PC @22:15:34.50 Elbow.reconfigure :loose @22:15:34.70 Forearm.reconfigure :tight @22:15:34.79 Forearm.seek PC @22:15:36.53 PC.seek :closed @22:15:40.51 Processing Clamp onSpring/offSpring for 8/23 samples, on/off torque 1.45 @22:15:40.65 Hand.open @22:15:41.51 Forearm.reconfigure :loose @22:15:41.59 Forearm.seek :retract @22:15:42.71 PSR.select :air @22:15:42.77 PSV.dial PSR @22:15:43.37 Pulling 0.5ml of PSR.flush @22:15:43.39 PSR.select :flush @22:15:43.58 PS.seek 0.5ml @22:15:49.60 delay 5 seconds @22:15:54.65 PSR.select :air @22:15:54.86 PS.seek 2.5ml @22:16:15.97 delay 1 second @22:16:17.01 PRV.dial :waste2,avoiding: :ab2 @22:16:18.89 PTV.dial :PRVmixing @22:16:20.14 PSV.dial :mixloop @22:16:21.17 PS.seek :empty @22:16:47.47 PSR.select :air @22:16:47.57 PSV.dial PSR @22:16:48.71 Pulling 0.5ml of PSR.flush @22:16:48.73 PSR.select :flush @22:16:48.91 PS.seek 0.5ml @22:16:54.83 delay 5 seconds @22:16:59.88 PSR.select :air @22:17:00.02 PS.seek 2.5ml @22:17:21.16 delay 1 second @22:17:22.20 PBV.dial :waste1 @22:17:22.59 PTV.dial :mixing @22:17:23.37 PSV.dial :mixloop @22:17:24.39 PS.seek :empty @22:17:49.58 upsync @22:17:50.57 PSV.dial PSR @22:17:51.56 PSR.select :flush @22:17:51.67 PS.seek 1ml @22:18:02.66 PSR.select :air @22:18:02.78 PS.seek 2.5ml @22:18:18.85 PSV.dial PTV @22:18:19.56 PTV.dial :puck @22:18:20.26 PBV.dial :waste1 @22:18:20.28 PS.seek 0ml @22:18:46.60 PSV.dial PSR @22:18:47.43 PSR.select :flush @22:18:47.55 PS.seek 0.5ml @22:18:53.51 PSR.select :air @22:18:53.62 PS.seek 2.5ml @22:19:14.66 PSV.dial PTV @22:19:15.48 PBV.dial PSV @22:19:16.01 PS.seek 0ml @22:19:42.19 PSV.dial PBV @22:19:42.62 PS.seek 2.5ml @22:20:08.75 PSV.dial :waste1 @22:20:09.46 PS.seek 0ml @22:20:35.69 PSV.dial PSR @22:20:36.13 PSR.select :air @22:20:36.15 PS.seek 2.5ml @22:21:02.41 PSV.dial PTV @22:21:03.11 PBV.dial PSV @22:21:03.13 PS.seek 0ml @22:21:20.92 CBV.dial :waste2b @22:21:21.29 CS.reconfigure :default @22:21:21.34 CS.seek 0ml @22:21:24.18 delay 1:00 @22:21:29.35 PSV.dial PBV @22:21:29.81 PS.seek 2.5ml @22:21:56.03 PSV.dial :waste1 @22:21:56.74 PS.seek 0ml @22:22:23.09 PSV.dial PSR @22:22:23.53 PSR.select :flush @22:22:23.65 PS.seek 1ml @22:22:24.20 CTV.dial :air,avoiding: :intake @22:22:25.43 Fix #2 of 2 (with RNAlater) for 10:00 @22:22:25.44 CSV.dial CSR @22:22:25.92 Pulling 1.999ml of CSR.RNAlater @22:22:25.93 CSR.select :RNAlater @22:22:26.05 CS.seek 2ml @22:22:31.93 delay 5 seconds @22:22:34.59 PSR.select :air @22:22:34.71 PS.seek 2.5ml @22:22:36.94 CSR.select :air @22:22:37.06 CS.reconfigure :fast @22:22:37.11 CS.seek 10ml @22:22:50.81 PSV.dial PTV @22:22:51.52 PTV.dial :puck @22:22:51.53 PBV.dial :waste1 @22:22:52.05 PS.seek 0ml @22:22:55.36 Pumping wcr puck from tube 2 up to 35psi... @22:22:55.38 CBV.dial SSV @22:22:56.65 CBV.dial between CSV and waste2b @22:22:57.58 CSV.dial between air and CTV @22:22:58.17 CS.seek 7.245ml @22:23:05.02 CTV.dial CSV,avoiding: :air @22:23:05.05 CSV.dial CTV @22:23:06.37 CS.reconfigure :pressurize @22:23:06.47 CS.seek 0ml @22:23:18.25 PSV.dial PSR @22:23:18.97 PSR.select :flush @22:23:19.09 PS.seek 0.5ml @22:23:23.10 delay 10:00 @22:23:25.12 PSR.select :air @22:23:25.24 PS.seek 2.5ml @22:23:46.35 PSV.dial PTV @22:23:47.06 PBV.dial PSV @22:23:47.60 PS.seek 0ml @22:24:13.81 PSV.dial PBV @22:24:14.27 PS.seek 2.5ml @22:24:40.50 PSV.dial :waste1 @22:24:41.21 PS.seek 0ml @22:25:07.37 PSV.dial PSR @22:25:07.81 PSR.select :air @22:25:07.83 PS.seek 2.5ml @22:25:34.09 PSV.dial PTV @22:25:34.79 PBV.dial PSV @22:25:34.81 PS.seek 0ml @22:26:00.96 PSV.dial PBV @22:26:01.42 PS.seek 2.5ml @22:26:27.68 PSV.dial :waste1 @22:26:28.39 PS.seek 0ml @22:26:54.65 Garaging FlushPuck in Processing Clamp @22:26:54.72 Elbow.reconfigure :tight @22:26:54.77 Elbow.seek PC @22:26:54.89 Elbow.reconfigure :loose @22:26:54.98 Forearm.seek PC @22:26:55.91 Hand.close @22:26:56.46 PC.seek :open @22:27:00.41 Forearm.seek :retract @22:27:01.51 Elbow.reconfigure :tight @22:27:01.55 Elbow.seek :garage @22:27:02.87 Elbow.reconfigure :loose @22:27:02.96 Forearm.reconfigure :tight @22:27:03.01 Forearm.seek :garage @22:27:04.08 Hand.open @22:27:04.89 Forearm.reconfigure :loose @22:27:04.93 Forearm.seek :retract @22:33:23.57 CBV.dial :waste2b @22:33:23.91 CS.reconfigure :default @22:33:23.96 CS.seek 0ml @22:33:26.78 delay 1:00 @22:34:26.81 CTV.dial :air,avoiding: :intake @22:34:28.11 Storing wcr puck in Collection Clamp to tube 1 @22:34:28.27 SC.reconfigure :tight @22:34:28.32 SC.seek 1 @22:34:28.38 SC.configure :limp @22:34:28.58 Elbow.reconfigure :tight @22:34:28.63 Elbow.seek 1 @22:34:30.47 Forearm.reconfigure :tight @22:34:30.52 Forearm.seek 1 + 700 counts @22:34:31.65 SE.reconfigure :pick @22:34:31.70 SE.seek :beamBreak @22:34:32.76 SE.configure :default @22:34:32.90 Elbow.reconfigure :limp @22:34:32.95 Forearm.reconfigure :loose @22:34:32.99 Forearm.seek 1 @22:34:33.41 Elbow.hold @22:34:33.48 Elbow.reconfigure :tight @22:34:33.57 SE.seek 30.41 Pucks below Top Plate @22:34:34.06 Forearm.force -20 @22:34:34.11 Forearm.seek 1 + 319 counts @22:34:34.50 Forearm.reconfigure :tight @22:34:34.55 Forearm.seek 1 + 700 counts @22:34:34.90 SE.seek 31.41 Pucks below Top Plate @22:34:35.50 Forearm.reconfigure :loose @22:34:35.55 Forearm.seek :retract @22:34:36.48 Elbow.seek CC @22:34:38.11 Elbow.reconfigure :loose @22:34:38.20 Forearm.seek CC @22:34:39.14 Hand.close @22:34:39.69 CC.seek :open @22:34:44.12 Forearm.seek :retract @22:34:45.19 Elbow.reconfigure :tight @22:34:45.24 Elbow.seek 1 @22:34:47.03 Elbow.reconfigure :limp @22:34:47.08 Forearm.seek 1 @22:34:48.18 Elbow.hold @22:34:48.25 Elbow.reconfigure :tight @22:34:48.29 Hand.open @22:34:49.19 Forearm.force -20 @22:34:49.22 Forearm.seek 1 + 320 counts @22:34:49.59 Forearm.reconfigure :tight @22:34:49.64 Forearm.seek 1 + 700 counts @22:34:49.90 SE.seek :down @22:34:50.50 SE.stop @22:34:51.03 Forearm.reconfigure :loose @22:34:51.08 Forearm.seek :retract @22:34:52.05 Starting finalPurge @22:34:52.07 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:34:52.24 Elbow.seek FlushPuck::Garage @22:34:54.19 Elbow.reconfigure :loose @22:34:54.28 Forearm.seek :garage @22:34:55.38 Hand.close @22:34:56.05 Forearm.seek :retract @22:34:57.12 Elbow.reconfigure :tight @22:34:57.16 Elbow.seek CC @22:35:00.46 Elbow.reconfigure :loose @22:35:00.55 Forearm.reconfigure :tight @22:35:00.59 Forearm.seek CC @22:35:01.84 CC.seek :closed @22:35:06.08 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.88 @22:35:06.15 Hand.open @22:35:07.00 Forearm.reconfigure :loose @22:35:07.04 Forearm.seek :retract @22:35:08.02 Flushing the Collection Valve::Manifold @22:35:08.14 CSV.dial CSR @22:35:08.62 Pulling 5ml of CSR.flush CSR.select :flush @22:35:08.74 CS.seek 5ml @22:35:22.16 delay 5 seconds @22:35:27.17 CSR.select :air @22:35:27.32 CS.reconfigure :fast @22:35:27.36 CS.seek 7.5ml @22:35:33.62 CSV.dial :waste @22:35:34.86 CS.reconfigure :default @22:35:34.90 CS.seek 0ml @22:35:54.69 Flushing lower manifold's waste1 port @22:35:54.80 CSV.dial :flush @22:35:55.24 CS.reconfigure :fast @22:35:55.28 CS.seek 5ml @22:36:06.87 delay 5 seconds @22:36:11.89 CSV.dial :air @22:36:12.38 CS.seek 7ml @22:36:17.63 CSV.dial CTV @22:36:17.67
CTV.dial CSV @22:36:18.95 CBV.dial :waste1 @22:36:19.67 CS.seek 0ml @22:36:35.66 Flushing lower manifold's waste2 port @22:36:35.77 CSV.dial :flush @22:36:36.47 CS.seek 5ml @22:36:48.09 delay 5 seconds @22:36:53.11 CSV.dial :air @22:36:53.60 CS.seek 7ml @22:36:58.81 CSV.dial CTV @22:36:58.85
CTV.dial CSV @22:36:59.25 CBV.dial :waste2 @22:37:00.25 CS.seek 0ml @22:37:16.15 Flushing lower manifold's waste2b port @22:37:16.27 CSV.dial :flush @22:37:16.97 CS.seek 5ml @22:37:28.62 delay 5 seconds @22:37:33.64 CSV.dial :air @22:37:34.13 CS.seek 7ml @22:37:39.28 CSV.dial CTV @22:37:39.30
CTV.dial CSV @22:37:39.73 CBV.dial :waste2b @22:37:40.70 CS.seek 0ml @22:37:56.53 Disinfecting external Intake with kill @22:37:56.64 CSV.dial CSR @22:37:57.13 Pulling 8ml of CSR.kill CSR.select :kill @22:37:57.24 CS.seek 8ml @22:38:15.28 delay 5 seconds @22:38:20.29 CSR.select :air @22:38:20.41 CS.seek 10ml @22:38:25.69 CSV.dial CBV @22:38:25.71
CBV.dial CSV @22:38:26.71 CTV.dial :intake @22:38:27.43 Intake.select :open @22:38:27.54 CS.seek 2ml @22:38:45.46 Intake.select :close @22:38:45.56 CTV.dial :air @22:38:46.28 CS.reconfigure :default @22:38:46.32 CS.seek 0ml @22:38:52.21 CSV.dial CSR @22:38:53.23 Pulling 5ml of CSR.flush @22:38:53.24 CSR.select :flush @22:38:53.35 CS.seek 5ml @22:39:06.78 delay 5 seconds @22:39:11.79 CSR.select :air @22:39:11.90 CS.seek 10ml @22:39:25.27 CSV.dial CBV @22:39:26.25 CS.seek 0ml @22:39:52.34 Garaging FlushPuck in Collection Clamp @22:39:52.40 Elbow.reconfigure :tight @22:39:52.45 Elbow.seek CC @22:39:52.55 Elbow.reconfigure :loose @22:39:52.63 Forearm.seek CC @22:39:53.65 Hand.close @22:39:54.78 CC.seek :open @22:39:59.10 Forearm.seek :retract @22:40:00.14 Elbow.reconfigure :tight @22:40:00.18 Elbow.seek :garage @22:40:03.21 Elbow.reconfigure :loose @22:40:03.30 Forearm.reconfigure :tight @22:40:03.34 Forearm.seek :garage @22:40:04.50 Hand.open @22:40:05.24 Forearm.reconfigure :loose @22:40:05.28 Forearm.seek :retract @22:40:06.37 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 20:15:05 @22:40:06.42 Asleep after completing DA_WCR_111122 assays @22:40:06.44 GoodNews.email "{1 => 32,\n 2 => 1,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:34:24, 24.5C, 28% humidity, 15.7psia, 14.036V, 0.312A, 0.312A avg, 4.38W\nSampled 700.0ml",Subject: "Asleep after completing DA_WCR_111122 assays" @22:40:40.57
Uploading Logs @22:43:14.94 upsync @15:58:10.84UTC01-Dec-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @15:58:13.37 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @15:58:17.29 Polling Can every 10:00 @15:58:17.31 Cleanly exited at 15:14:50UTC01-Dec-22 @15:58:17.35 Loading /home/esp/esp2/mission/DA_WCR_120122.rb mission @15:58:17.41 Preparing to sleep until + 00:00:05 @15:58:17.43 GoodNews.email "Starting DA_WCR_120122 w/pucks in tube 3\n{1 => 32,\n 2 => 1,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:58:17, 22.6C, 32% humidity, 15.6psia, 14.075V, 0.214A, 0.214A avg, 3.02W",Subject: "DA_WCR_120122 begun" @15:58:17.49
Asleep til + 00:00:05 @15:58:17.51 Continuing at 15:58 @15:58:17.77 Gate.power :core,:OFF @15:58:17.78 Gate.power :raw,:OFF @15:58:17.79 Shutdown @15:58:22.38
Powering core bus @15:58:23.57 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_120122.rb:14\n{1 => 28,\n 2 => 9,\n 3 => 29,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:58:17, 22.6C, 33% humidity, 15.6psia, 14.065V, 0.214A, 0.214A avg, 3.01W",Subject: "Asleep until 15:58" @15:58:27.66
Gate.power :raw,:ON @15:58:29.19 CS.configure :default @15:58:29.37 PS.configure :default @15:58:29.55 SS.configure :default @15:58:29.81 CC.configure :default @15:58:30.00 PC.configure :default @15:58:30.24 Forearm.configure :loose @15:58:30.45 Elbow.configure :loose @15:58:30.67 SC.configure :loose @15:58:30.88 SE.configure :default @15:58:30.96 CH.coast @15:58:31.06 CH.configure :default @15:58:31.12 PH.coast @15:58:31.28 PH.configure :default @15:58:31.37 All dwarf controllers running firmware version 3.85 @15:58:31.53 Valve.reset @15:58:31.86 Intake.select :close @15:58:31.98 Exhaust.select :close @15:58:32.08 CTV.dial :air @15:58:32.28 PSV.dial :air @15:58:32.38 CSV.dial :waste1 @15:58:32.56
Forearm.reconfigure :loose @15:58:32.70 Forearm.home.seek :retract @15:58:32.91 SSV.dial :spit,avoiding: :exhaust @15:58:33.44 PS.reconfigure :default @15:58:33.56 PS.home.seek 0ml @15:58:33.68 Hand.close @15:58:33.85
Elbow.jog 1500 @15:58:34.01 CS.reconfigure :fast @15:58:34.09 SS.reconfigure :default @15:58:34.23 SS.home.seek 0ml @15:58:34.29 CS.home.seek 0ml @15:58:34.52
Elbow.reconfigure :loose @15:58:34.60 Elbow.home.seek :clear @15:58:39.07 SE.reconfigure :default @15:58:39.15 SE.home.seek :down @15:58:39.86 SC.reconfigure :tight @15:58:39.94 SC.home.seek :initial @15:58:42.33 PS.stop @15:59:10.03 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @15:59:10.05
Retry #1 of 3 @15:59:10.17 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @15:59:14.49 SC.configure :limp @15:59:14.63 Forearm.seek :retract @15:59:14.79 Elbow.seek :hammer @15:59:16.05 Forearm.reconfigure :tight @15:59:16.22 Forearm.seek retracted @15:59:16.67 PC.reconfigure :default @15:59:16.72
CC.reconfigure :default @15:59:16.88 PC.home.seek :open @15:59:16.94
CC.home.seek :open @15:59:20.74 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @15:59:20.78 Notice.email "At 15:59:20 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_120122.rb:14\n{1 => 28,\n 2 => 9,\n 3 => 29,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:59:20, 22.5C, 33% humidity, 15.6psia, 14.031V, 0.257A, 0.257A avg, 3.61W",Subject: "Started DA_WCR_120122 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @15:59:20.84
Starting initialPurge @15:59:20.86 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:59:21.00 Hand.open @15:59:22.00 Elbow.reconfigure :tight @15:59:22.12 Elbow.seek FlushPuck::Garage @15:59:24.13 Elbow.reconfigure :loose @15:59:24.51 Forearm.reconfigure :loose @15:59:24.57 Forearm.seek :garage @15:59:25.73 Hand.close @15:59:26.60 Forearm.seek :retract @15:59:27.81 Elbow.reconfigure :tight @15:59:27.89 Elbow.seek CC @15:59:30.86 Elbow.reconfigure :loose @15:59:31.05 Forearm.reconfigure :tight @15:59:31.11 Forearm.seek CC @15:59:32.85 CC.seek :closed @15:59:37.19 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.85 @15:59:37.35 Hand.open @15:59:38.21 Forearm.reconfigure :loose @15:59:38.29 Forearm.seek :retract @15:59:39.40 Flushing the Collection Valve::Manifold @15:59:39.63 CSV.dial CSR @15:59:41.04 Pulling 5ml of CSR.flush @15:59:41.09 CSR.select :flush @15:59:41.29 CS.reconfigure :default @15:59:41.35 CS.seek 5ml @15:59:54.86 delay 5 seconds @15:59:59.90 CSR.select :air @16:00:00.14 CS.reconfigure :fast @16:00:00.25 CS.seek 7.501ml @16:00:06.59 CSV.dial :waste @16:00:08.00 CS.reconfigure :default @16:00:08.08 CS.seek 0ml @16:00:27.92 Purging external Intake @16:00:28.17 CSV.dial :flush @16:00:28.73 CS.reconfigure :fast @16:00:28.81 CS.seek 10ml @16:00:30.82 upsync @16:00:51.40
delay 5 seconds @16:00:56.42 CSV.dial CBV @16:00:56.46
CBV.dial CSV @16:00:57.21 CTV.dial :intake @16:00:57.93 Intake.select :open @16:00:58.04 CS.seek 0ml @16:01:20.52 Intake.select :close @16:01:20.62 CTV.dial :air @16:01:21.37 Flushing upper manifold @16:01:21.48 CSV.dial :flush @16:01:22.20 CS.seek 5ml @16:01:33.93 delay 5 seconds @16:01:38.95 CSV.dial :air @16:01:39.44 CS.seek 7ml @16:01:44.69 CSV.dial CBV @16:01:44.71
CBV.dial CSV @16:01:45.71 CTV.dial :air @16:01:45.73 CS.seek 0ml @16:02:01.65 delay 7 seconds @16:02:08.67 CS.seek 3ml @16:02:16.05 CSV.dial :waste @16:02:16.50 CS.seek 0ml @16:02:23.94 CSV.dial :flush @16:02:24.37 CS.seek 3ml @16:02:31.74 delay 5 seconds @16:02:36.76 CSV.dial :air @16:02:37.25 CS.seek 4.999ml @16:02:42.61 CSV.dial :air @16:02:42.64 CS.seek 0ml @16:02:54.33 delay 7 seconds @16:03:01.36 CS.seek 3ml @16:03:08.68 CSV.dial :waste @16:03:09.39 CS.seek 0ml @16:03:16.71 Garaging FlushPuck in Collection Clamp @16:03:16.78 Elbow.reconfigure :tight @16:03:16.82 Elbow.seek CC @16:03:16.92 Elbow.reconfigure :loose @16:03:17.01 Forearm.seek CC @16:03:17.95 Hand.close @16:03:18.70 CC.seek :open @16:03:23.04 Forearm.seek :retract @16:03:24.11 Elbow.reconfigure :tight @16:03:24.15 Elbow.seek :garage @16:03:27.19 Elbow.reconfigure :loose @16:03:27.28 Forearm.reconfigure :tight @16:03:27.32 Forearm.seek :garage @16:03:28.46 Hand.open @16:03:29.60 Forearm.reconfigure :loose @16:03:29.71 Forearm.seek :retract @16:03:30.77 da sampling at most 1000ml, wcr at most 1000ml @16:03:30.80 Loading sh1 puck from tube 3 into Collection Clamp @16:03:30.81 DAfans.email "Started da in tube 3 at 16:03:30UTC01-Dec-22\n{1 => 28,\n 2 => 9,\n 3 => 29,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:59:20, 22.5C, 33% humidity, 15.6psia, 14.031V, 0.257A, 0.257A avg, 3.61W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @16:03:31.06
SC.reconfigure :tight @16:03:31.10 SC.seek 3 @16:03:41.04 SC.configure :limp @16:03:41.55 Elbow.reconfigure :tight @16:03:41.64 Elbow.seek 3 @16:03:43.86 Forearm.reconfigure :tight @16:03:43.96 Forearm.seek 3 + 700 counts @16:03:45.09 SE.reconfigure :pick @16:03:45.19 SE.seek :beamBreak @16:03:46.26 SE.configure :default @16:03:46.45 SE.seek :down @16:03:47.04 SE.stop @16:03:47.26 Forearm.reconfigure :loose @16:03:47.34 Forearm.seek :retract @16:03:48.41 Puck::Error in MAIN -- 33 pucks loaded in tube 3 when expecting 29 @16:03:48.43 Waiting to Resume from Checkpoint "Loading sh1 puck from tube 3 into Collection Clamp" @16:03:48.45 BadNews.email "33 pucks loaded in tube 3 when expecting 29",Subject: "Puck::Error in MAIN" @16:05:10.65 Session Begun @16:05:35.81 upsync @16:05:39.70 -> abort! 'mission file incorrect' @16:05:39.71 CmdServer::Client::Error in dave -- Aborting Mission: mission file incorrect @16:05:39.75 BadNews.email "mission file incorrect",Subject: "DA_WCR_120122 ABORTED!" @16:05:51.06 -> exit @16:05:51.08 Session Ended @16:06:18.38
Uploading Logs @16:10:50.76UTC01-Dec-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @16:10:53.48 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @16:10:57.44 Polling Can every 10:00 @16:10:57.47 Cleanly exited at 16:10:31UTC01-Dec-22 @16:10:57.51 Loading /home/esp/esp2/mission/DA_WCR_120122.rb mission @16:10:57.56 GoodNews.email "Starting DA_WCR_120122 w/pucks in tube 3\n{1 => 32,\n 2 => 1,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:10:57, 22.6C, 32% humidity, 15.6psia, 14.050V, 0.257A, 0.251A avg, 3.61W",Subject: "DA_WCR_120122 begun" @16:10:57.60
Preparing to sleep until + 00:00:05 @16:10:57.63 Asleep til + 00:00:05 @16:10:57.64 Continuing at 16:11 @16:10:57.93 Gate.power :core,:OFF @16:10:57.94 Gate.power :raw,:OFF @16:10:57.95 Shutdown @16:11:02.54
Powering core bus @16:11:08.00 Gate.power :raw,:ON @16:11:08.71 CS.configure :default @16:11:08.80 PS.configure :default @16:11:08.88 SS.configure :default @16:11:08.97 CC.configure :default @16:11:09.05 PC.configure :default @16:11:09.16 Forearm.configure :loose @16:11:09.24 Elbow.configure :loose @16:11:09.35 SC.configure :loose @16:11:09.43 SE.configure :default @16:11:09.47 CH.coast @16:11:09.52 CH.configure :default @16:11:09.55 PH.coast @16:11:09.60 PH.configure :default @16:11:09.64 All dwarf controllers running firmware version 3.85 @16:11:09.74 Valve.reset @16:11:10.02 PSV.dial :air @16:11:10.05 Intake.select :close @16:11:10.07 Exhaust.select :close @16:11:10.10 CTV.dial :air @16:11:10.19 PS.reconfigure :default @16:11:10.29 PS.home.seek 0ml @16:11:10.32 CSV.dial :waste1 @16:11:10.36
Forearm.reconfigure :loose @16:11:10.44 Forearm.home.seek :retract @16:11:10.68 SSV.dial :spit,avoiding: :exhaust @16:11:10.81 CS.reconfigure :fast @16:11:10.84 SS.reconfigure :default @16:11:10.93 CS.home.seek 0ml @16:11:10.96 SS.home.seek 0ml @16:11:11.25 Hand.close @16:11:11.28
Elbow.jog 1500 @16:11:11.67 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_120122.rb:14\n{1 => 32,\n 2 => 1,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:10:57, 22.6C, 32% humidity, 15.6psia, 14.050V, 0.257A, 0.251A avg, 3.61W",Subject: "Asleep until 16:11" @16:11:11.82
Elbow.reconfigure :loose @16:11:11.95 Elbow.home.seek :clear @16:11:16.43 SE.reconfigure :default @16:11:16.51 SE.home.seek :down @16:11:17.17 SC.reconfigure :tight @16:11:17.25 SC.home.seek :initial @16:11:19.05 PS.stop @16:11:47.45 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @16:11:47.47
Retry #1 of 3 @16:11:47.61 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @16:11:51.84 SC.configure :limp @16:11:51.93 Forearm.seek :retract @16:11:52.05 Elbow.seek :hammer @16:11:53.26 Forearm.reconfigure :tight @16:11:53.37 Forearm.seek retracted @16:11:53.99 PC.reconfigure :default @16:11:54.05
CC.reconfigure :default @16:11:54.23 PC.home.seek :open @16:11:54.29
CC.home.seek :open @16:11:58.14 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @16:11:58.16 Notice.email "At 16:11:57 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_120122.rb:14\n{1 => 32,\n 2 => 1,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:11:58, 22.6C, 32% humidity, 15.6psia, 14.036V, 0.251A, 0.251A avg, 3.52W",Subject: "Started DA_WCR_120122 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @16:11:58.20
Starting initialPurge @16:11:58.26 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:11:58.39 Hand.open @16:11:59.43 Elbow.reconfigure :tight @16:11:59.51 Elbow.seek FlushPuck::Garage @16:12:01.53 Elbow.reconfigure :loose @16:12:01.76 Forearm.reconfigure :loose @16:12:01.88 Forearm.seek :garage @16:12:02.97 Hand.close @16:12:03.95 Forearm.seek :retract @16:12:05.11 Elbow.reconfigure :tight @16:12:05.20 Elbow.seek CC @16:12:08.23 Elbow.reconfigure :loose @16:12:08.41 Forearm.reconfigure :tight @16:12:08.49 Forearm.seek CC @16:12:10.07 CC.seek :closed @16:12:14.45 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.85 @16:12:14.58 Hand.open @16:12:15.51 Forearm.reconfigure :loose @16:12:15.59 Forearm.seek :retract @16:12:16.61 Flushing the Collection Valve::Manifold @16:12:16.83 CSV.dial CSR @16:12:18.25 Pulling 5ml of CSR.flush @16:12:18.27 CSR.select :flush @16:12:18.46 CS.reconfigure :default @16:12:18.58 CS.seek 5ml @16:12:32.10 delay 5 seconds @16:12:37.14 CSR.select :air @16:12:37.37 CS.reconfigure :fast @16:12:37.44 CS.seek 7.5ml @16:12:43.93 CSV.dial :waste @16:12:45.39 CS.reconfigure :default @16:12:45.48 CS.seek 0ml @16:13:05.18 Purging external Intake @16:13:05.40 CSV.dial :flush @16:13:05.91 CS.reconfigure :fast @16:13:05.99 CS.seek 10ml @16:13:17.39 upsync @16:13:28.47
delay 5 seconds @16:13:33.49 CSV.dial CBV @16:13:33.52
CBV.dial CSV @16:13:34.27 CTV.dial :intake @16:13:34.98 Intake.select :open @16:13:35.09 CS.seek 0ml @16:13:57.50 Intake.select :close @16:13:57.60 CTV.dial :air @16:13:58.33 Flushing upper manifold @16:13:58.44 CSV.dial :flush @16:13:59.16 CS.seek 5ml @16:14:10.84 delay 5 seconds @16:14:15.86 CSV.dial :air @16:14:16.36 CS.seek 6.999ml @16:14:21.51 CSV.dial CBV @16:14:21.53
CBV.dial CSV @16:14:22.64 CTV.dial :air @16:14:22.66 CS.seek 0ml @16:14:38.62 delay 7 seconds @16:14:45.65 CS.seek 3ml @16:14:53.12 CSV.dial :waste @16:14:53.55 CS.seek 0ml @16:15:00.94 CSV.dial :flush @16:15:01.38 CS.seek 3ml @16:15:08.69 delay 5 seconds @16:15:13.70 CSV.dial :air @16:15:14.20 CS.seek 5ml @16:15:19.37 CSV.dial :air @16:15:19.39 CS.seek 0ml @16:15:31.06 delay 7 seconds @16:15:38.09 CS.seek 3ml @16:15:45.53 CSV.dial :waste @16:15:46.24 CS.seek 0ml @16:15:53.61 Garaging FlushPuck in Collection Clamp @16:15:53.73 Elbow.reconfigure :tight @16:15:53.81 Elbow.seek CC @16:15:54.11 Elbow.reconfigure :loose @16:15:54.19 Forearm.seek CC @16:15:55.16 Hand.close @16:15:55.87 CC.seek :open @16:16:00.20 Forearm.seek :retract @16:16:01.20 Elbow.reconfigure :tight @16:16:01.24 Elbow.seek :garage @16:16:04.28 Elbow.reconfigure :loose @16:16:04.37 Forearm.reconfigure :tight @16:16:04.41 Forearm.seek :garage @16:16:05.53 Hand.open @16:16:06.30 Forearm.reconfigure :loose @16:16:06.75 Forearm.seek :retract @16:16:07.79 da sampling at most 1000ml, wcr at most 1000ml @16:16:07.82 DAfans.email "Started da in tube 3 at 16:16:07UTC01-Dec-22\n{1 => 32,\n 2 => 1,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@16:11:58, 22.6C, 32% humidity, 15.6psia, 14.036V, 0.251A, 0.251A avg, 3.52W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @16:16:07.84
Loading sh1 puck from tube 3 into Collection Clamp @16:16:08.08 SC.reconfigure :tight @16:16:08.12 SC.seek 3 @16:16:17.97 SC.configure :limp @16:16:18.22 Elbow.reconfigure :tight @16:16:18.26 Elbow.seek 3 @16:16:20.31 Forearm.reconfigure :tight @16:16:20.40 Forearm.seek 3 + 700 counts @16:16:21.58 SE.reconfigure :pick @16:16:21.69 SE.seek :beamBreak @16:16:22.76 SE.configure :default @16:16:23.05 Elbow.seek 3 @16:16:23.41 Forearm.seek 3 + 300 counts @16:16:23.66 Forearm.reconfigure :loose @16:16:23.72 Hand.close @16:16:24.42 SE.seek :down @16:16:24.88
Forearm.seek :retract @16:16:25.24 SE.stop @16:16:25.90
Elbow.seek CC @16:16:27.63 Elbow.reconfigure :loose @16:16:27.81 Forearm.reconfigure :tight @16:16:27.89 Forearm.seek CC @16:16:29.58 CC.seek :closed @16:16:34.01 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.61 @16:16:34.03 Hand.open @16:16:34.92 Forearm.reconfigure :loose @16:16:35.00 Forearm.seek :retract @16:16:36.12 collectSample 1000ml w/bubble point of 24psi @16:16:36.14 Priming 7ml @16:16:36.16 Intake.select :close @16:16:36.18 Exhaust.select :close @16:16:36.20 SS.hold @16:16:36.58 Calibrating pressure and temperature sensors @16:16:36.64 CTV.dial :air @16:16:36.66 SSV.dial :spit,avoiding: :exhaust @16:16:36.77 SS.reconfigure :fast @16:16:36.85 SS.seek 5ml @16:16:39.78 SSV.dial CBV @16:16:39.86
CBV.dial SSV @16:16:41.17 delay 3.8 seconds @16:16:45.23 SSV.dial :spit @16:16:46.53 SS.seek 0ml @16:16:49.07 SSV.dial CBV @16:16:50.47 CTV.dial :intake @16:16:51.30 SSV.dial CBV @16:16:51.34
CBV.dial SSV @16:16:51.36 Allowing 40s for 7.0ml of remaining 7.0ml @16:16:51.38 Intake.select :open @16:16:53.63 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:16:53.71 SS.reconfigure :inhaling 2.14ml/s @16:16:53.80 SS.seek 7ml @16:17:00.48 Stroke completed -- waiting for pressure to equalize ... @16:17:00.51 SS.reconfigure :exhale @16:17:00.58 SS.hold @16:17:17.71 Intake.select :close @16:17:17.99 SSV.dial :exhaust @16:17:18.75 Exhaust.select :open @16:17:18.91 SS.seek 0ml @16:17:23.12 Exhaust.select :close @16:17:23.26 Sampling 1000.0ml @16:17:23.28 Intake.select :close @16:17:23.30 Exhaust.select :close @16:17:23.33 SS.reconfigure :default @16:17:23.40 SS.hold @16:17:24.78 CTV.dial :intake @16:17:24.82 SSV.dial CBV @16:17:24.88
CBV.dial SSV @16:17:25.65 Allowing 130s for 25.0ml of remaining 1000.0ml @16:17:25.67 Intake.select :open @16:17:27.94 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:17:27.97 SS.reconfigure :inhaling 2.14ml/s @16:17:28.04 SS.seek 25ml @16:17:31.68 Vacuum too high -- waiting up to 40 seconds @16:17:31.70 SS.reconfigure :exhale @16:17:31.80 SS.hold @16:17:34.38 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:17:34.42 SS.reconfigure :inhaling 1.93ml/s @16:17:34.48 SS.seek 25ml @16:17:36.27 Vacuum too high -- waiting up to 40 seconds @16:17:36.29 SS.reconfigure :exhale @16:17:36.38 SS.hold @16:17:38.97 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:17:39.01 SS.reconfigure :inhaling 1.74ml/s @16:17:39.08 SS.seek 25ml @16:17:41.30 Vacuum too high -- waiting up to 40 seconds @16:17:41.32 SS.reconfigure :exhale @16:17:41.40 SS.hold @16:17:43.50 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:17:43.54 SS.reconfigure :inhaling 1.57ml/s @16:17:43.62 SS.seek 25ml @16:17:45.58 Vacuum too high -- waiting up to 40 seconds @16:17:45.60 SS.reconfigure :exhale @16:17:45.70 SS.hold @16:17:47.30 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:17:47.32 SS.reconfigure :inhaling 1.41ml/s @16:17:47.40 SS.seek 25ml @16:17:49.34 Vacuum too high -- waiting up to 40 seconds @16:17:49.37 SS.reconfigure :exhale @16:17:49.44 SS.hold @16:17:50.91 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:17:50.95 SS.reconfigure :inhaling 1.27ml/s @16:17:51.03 SS.seek 25ml @16:17:53.21 Vacuum too high -- waiting up to 40 seconds @16:17:53.23 SS.reconfigure :exhale @16:17:53.31 SS.hold @16:17:54.77 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:17:54.81 SS.reconfigure :inhaling 1.14ml/s @16:17:54.89 SS.seek 25ml @16:17:56.57 Vacuum too high -- waiting up to 40 seconds @16:17:56.59 SS.reconfigure :exhale @16:17:56.68 SS.hold @16:17:58.00 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:17:58.04 SS.reconfigure :inhaling 1.03ml/s @16:17:58.13 SS.seek 25ml @16:18:00.11 Vacuum too high -- waiting up to 40 seconds @16:18:00.13 SS.reconfigure :exhale @16:18:00.23 SS.hold @16:18:01.52 SS.reconfigure :inhaling 0.93ml/s @16:18:01.60 SS.seek 25ml @16:18:03.54 Vacuum too high -- waiting up to 40 seconds @16:18:03.56 SS.reconfigure :exhale @16:18:03.62 SS.hold @16:18:04.76 SS.reconfigure :inhaling 0.84ml/s @16:18:04.86 SS.seek 25ml @16:18:06.72 Vacuum too high -- waiting up to 40 seconds @16:18:06.73 SS.reconfigure :exhale @16:18:06.84 SS.hold @16:18:08.44 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:18:08.48 SS.reconfigure :inhaling 0.76ml/s @16:18:08.58 SS.seek 25ml @16:18:10.29 Vacuum too high -- waiting up to 40 seconds @16:18:10.31 SS.reconfigure :exhale @16:18:10.39 SS.hold @16:18:11.83 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:18:11.87 SS.reconfigure :inhaling 0.69ml/s @16:18:11.95 SS.seek 25ml @16:18:14.49 Vacuum too high -- waiting up to 40 seconds @16:18:14.51 SS.reconfigure :exhale @16:18:14.66 SS.hold @16:18:15.79 Intake @14.0psia, vacuum from 8.5 to 11.5psi @16:18:15.81 SS.reconfigure :inhaling 0.61ml/s @16:18:15.89 SS.seek 25ml @16:18:18.69 Vacuum too high -- waiting up to 40 seconds @16:18:18.71 SS.reconfigure :exhale @16:18:18.80 SS.hold @16:18:20.25 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:18:20.29 SS.reconfigure :inhaling 0.56ml/s @16:18:20.39 SS.seek 25ml @16:18:22.52 Vacuum too high -- waiting up to 40 seconds @16:18:22.54 SS.reconfigure :exhale @16:18:22.64 SS.hold @16:18:23.77 Intake @14.2psia, vacuum from 8.7 to 11.7psi @16:18:23.79 SS.reconfigure :inhaling 0.50ml/s @16:18:24.90 SS.seek 25ml @16:18:28.52 Vacuum too high -- waiting up to 40 seconds @16:18:28.54 SS.reconfigure :exhale @16:18:28.62 SS.hold @16:18:29.80 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:18:29.82 SS.reconfigure :inhaling 0.46ml/s @16:18:29.92 SS.seek 25ml @16:18:34.23 Vacuum too high -- waiting up to 40 seconds @16:18:34.24 SS.reconfigure :exhale @16:18:34.29 SS.hold @16:18:34.39 upsync @16:18:35.25
Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:18:35.27 SS.reconfigure :inhaling 0.41ml/s @16:18:35.31 SS.seek 25ml @16:18:42.29 Vacuum too high -- waiting up to 40 seconds @16:18:42.30 SS.reconfigure :exhale @16:18:42.34 SS.hold @16:18:43.61 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:18:43.62 SS.reconfigure :inhaling 0.37ml/s @16:18:43.67 SS.seek 25ml @16:18:49.52 Stroke completed -- waiting for pressure to equalize ... @16:18:49.53 SS.reconfigure :exhale @16:18:49.57 SS.hold @16:18:51.51 Intake.select :close @16:18:51.65 SSV.dial :exhaust @16:18:52.38 Exhaust.select :open @16:18:52.48 SS.seek 0ml @16:19:01.41 Exhaust.select :close @16:19:01.51 SSV.dial CBV @16:19:01.55
CBV.dial SSV @16:19:02.28 Allowing 130s for 25.0ml of remaining 975.0ml @16:19:02.29 Intake.select :open @16:19:04.45 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:19:04.46 SS.reconfigure :inhaling 0.37ml/s @16:19:04.51 SS.seek 25ml @16:19:50.91 Vacuum too high -- waiting up to 40 seconds @16:19:50.92 SS.reconfigure :exhale @16:19:50.96 SS.hold @16:19:51.80 Intake @13.8psia, vacuum from 8.3 to 11.3psi @16:19:51.82 SS.reconfigure :inhaling 0.33ml/s @16:19:51.86 SS.seek 25ml @16:20:24.20 Stroke completed -- waiting for pressure to equalize ... @16:20:24.21 SS.reconfigure :exhale @16:20:24.25 SS.hold @16:20:26.20 Intake.select :close @16:20:26.33 SSV.dial :exhaust @16:20:27.06 Exhaust.select :open @16:20:27.17 SS.seek 0ml @16:20:36.01 Exhaust.select :close @16:20:36.12 SSV.dial CBV @16:20:36.14
CBV.dial SSV @16:20:36.89 Allowing 130s for 25.0ml of remaining 950.0ml @16:20:36.90 Intake.select :open @16:20:39.05 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:20:39.07 SS.reconfigure :inhaling 0.33ml/s @16:20:39.11 SS.seek 25ml @16:22:03.82 Stroke completed -- waiting for pressure to equalize ... @16:22:03.83 SS.reconfigure :exhale @16:22:03.87 SS.hold @16:22:06.26 Intake.select :close @16:22:06.40 SSV.dial :exhaust @16:22:07.14 Exhaust.select :open @16:22:07.24 SS.seek 0ml @16:22:16.62 Exhaust.select :close @16:22:16.72 SSV.dial CBV @16:22:16.75
CBV.dial SSV @16:22:17.50 Allowing 130s for 25.0ml of remaining 925.0ml @16:22:17.51 Intake.select :open @16:22:19.66 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:22:19.67 SS.reconfigure :inhaling 0.33ml/s @16:22:19.72 SS.seek 25ml @16:23:43.68 Stroke completed -- waiting for pressure to equalize ... @16:23:43.69 SS.reconfigure :exhale @16:23:43.73 SS.hold @16:23:46.62 Intake.select :close @16:23:46.76 SSV.dial :exhaust @16:23:47.48 Exhaust.select :open @16:23:47.58 SS.seek 0ml @16:23:56.98 Exhaust.select :close @16:23:57.08 SSV.dial CBV @16:23:57.11
CBV.dial SSV @16:23:57.85 Allowing 130s for 25.0ml of remaining 900.0ml @16:23:57.86 Intake.select :open @16:24:00.01 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:24:00.03 SS.reconfigure :inhaling 0.33ml/s @16:24:00.07 SS.seek 25ml @16:25:23.63 Stroke completed -- waiting for pressure to equalize ... @16:25:23.64 SS.reconfigure :exhale @16:25:23.68 SS.hold @16:25:26.65 Intake.select :close @16:25:26.79 SSV.dial :exhaust @16:25:27.50 Exhaust.select :open @16:25:27.61 SS.seek 0ml @16:25:36.89 Exhaust.select :close @16:25:37.00 SSV.dial CBV @16:25:37.03
CBV.dial SSV @16:25:37.77 Allowing 130s for 25.0ml of remaining 875.0ml @16:25:37.78 Intake.select :open @16:25:39.93 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:25:39.95 SS.reconfigure :inhaling 0.33ml/s @16:25:39.99 SS.seek 25ml @16:27:04.31 Stroke completed -- waiting for pressure to equalize ... @16:27:04.32 SS.reconfigure :exhale @16:27:04.82 SS.hold @16:27:07.65 Intake.select :close @16:27:07.79 SSV.dial :exhaust @16:27:08.52 Exhaust.select :open @16:27:08.62 SS.seek 0ml @16:27:17.86 Exhaust.select :close @16:27:17.96 SSV.dial CBV @16:27:17.99
CBV.dial SSV @16:27:18.72 Allowing 130s for 25.0ml of remaining 850.0ml @16:27:18.73 Intake.select :open @16:27:20.88 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:27:20.90 SS.reconfigure :inhaling 0.33ml/s @16:27:20.95 SS.seek 25ml @16:28:45.21 Stroke completed -- waiting for pressure to equalize ... @16:28:45.22 SS.reconfigure :exhale @16:28:45.27 SS.hold @16:28:48.15 Intake.select :close @16:28:48.28 SSV.dial :exhaust @16:28:49.01 Exhaust.select :open @16:28:49.12 SS.seek 0ml @16:28:58.07 Exhaust.select :close @16:28:58.18 SSV.dial CBV @16:28:58.21
CBV.dial SSV @16:28:58.95 Allowing 130s for 25.0ml of remaining 825.0ml @16:28:58.96 Intake.select :open @16:29:01.11 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:29:01.13 SS.reconfigure :inhaling 0.33ml/s @16:29:01.17 SS.seek 25ml @16:30:26.52 Stroke completed -- waiting for pressure to equalize ... @16:30:26.53 SS.reconfigure :exhale @16:30:26.57 SS.hold @16:30:30.03 Intake.select :close @16:30:30.16 SSV.dial :exhaust @16:30:30.89 Exhaust.select :open @16:30:30.99 SS.seek 0ml @16:30:40.31 Exhaust.select :close @16:30:40.41 SSV.dial CBV @16:30:40.44
CBV.dial SSV @16:30:41.18 Allowing 130s for 25.0ml of remaining 800.0ml @16:30:41.19 Intake.select :open @16:30:43.34 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:30:43.36 SS.reconfigure :inhaling 0.33ml/s @16:30:43.40 SS.seek 25ml @16:32:07.24 Stroke completed -- waiting for pressure to equalize ... @16:32:07.25 SS.reconfigure :exhale @16:32:07.29 SS.hold @16:32:11.35 Intake.select :close @16:32:11.48 SSV.dial :exhaust @16:32:12.21 Exhaust.select :open @16:32:12.32 SS.seek 0ml @16:32:21.77 Exhaust.select :close @16:32:21.87 SSV.dial CBV @16:32:21.90
CBV.dial SSV @16:32:22.65 Allowing 130s for 25.0ml of remaining 775.0ml @16:32:22.66 Intake.select :open @16:32:24.81 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:32:24.83 SS.reconfigure :inhaling 0.33ml/s @16:32:24.87 SS.seek 25ml @16:33:49.78 Stroke completed -- waiting for pressure to equalize ... @16:33:49.79 SS.reconfigure :exhale @16:33:49.83 SS.hold @16:33:53.88 Intake.select :close @16:33:54.02 SSV.dial :exhaust @16:33:54.74 Exhaust.select :open @16:33:54.85 SS.seek 0ml @16:34:04.19 Exhaust.select :close @16:34:04.29 SSV.dial CBV @16:34:04.32
CBV.dial SSV @16:34:05.05 Allowing 130s for 25.0ml of remaining 750.0ml @16:34:05.06 Intake.select :open @16:34:07.21 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:34:07.23 SS.reconfigure :inhaling 0.33ml/s @16:34:07.27 SS.seek 25ml @16:35:32.47 Stroke completed -- waiting for pressure to equalize ... @16:35:32.48 SS.reconfigure :exhale @16:35:32.52 SS.hold @16:35:37.28 Intake.select :close @16:35:37.41 SSV.dial :exhaust @16:35:38.14 Exhaust.select :open @16:35:38.25 SS.seek 0ml @16:35:47.17 Exhaust.select :close @16:35:47.27 SSV.dial CBV @16:35:47.30
CBV.dial SSV @16:35:48.05 Allowing 130s for 25.0ml of remaining 725.0ml @16:35:48.06 Intake.select :open @16:35:50.21 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:35:50.22 SS.reconfigure :inhaling 0.33ml/s @16:35:50.27 SS.seek 25ml @16:37:03.73 Vacuum too high -- waiting up to 40 seconds @16:37:03.74 SS.reconfigure :exhale @16:37:03.78 SS.hold @16:37:05.06 Intake @13.8psia, vacuum from 8.3 to 11.3psi @16:37:05.08 SS.reconfigure :inhaling 0.30ml/s @16:37:05.12 SS.seek 25ml @16:37:20.14 Stroke completed -- waiting for pressure to equalize ... @16:37:20.15 SS.reconfigure :exhale @16:37:20.19 SS.hold @16:37:23.73 Intake.select :close @16:37:23.87 SSV.dial :exhaust @16:37:24.59 Exhaust.select :open @16:37:24.70 SS.seek 0ml @16:37:33.75 Exhaust.select :close @16:37:33.85 SSV.dial CBV @16:37:33.88
CBV.dial SSV @16:37:34.62 Allowing 130s for 25.0ml of remaining 700.0ml @16:37:34.63 Intake.select :open @16:37:36.78 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:37:36.80 SS.reconfigure :inhaling 0.30ml/s @16:37:36.84 SS.seek 25ml @16:39:11.73 Stroke completed -- waiting for pressure to equalize ... @16:39:11.74 SS.reconfigure :exhale @16:39:11.78 SS.hold @16:39:15.93 Intake.select :close @16:39:16.06 SSV.dial :exhaust @16:39:16.79 Exhaust.select :open @16:39:16.90 SS.seek 0ml @16:39:25.85 Exhaust.select :close @16:39:25.95 SSV.dial CBV @16:39:25.98
CBV.dial SSV @16:39:26.71 Allowing 130s for 25.0ml of remaining 675.0ml @16:39:26.72 Intake.select :open @16:39:28.87 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:39:28.89 SS.reconfigure :inhaling 0.30ml/s @16:39:28.93 SS.seek 25ml @16:41:04.93 Stroke completed -- waiting for pressure to equalize ... @16:41:04.94 SS.reconfigure :exhale @16:41:04.98 SS.hold @16:41:09.11 Intake.select :close @16:41:09.25 SSV.dial :exhaust @16:41:09.97 Exhaust.select :open @16:41:10.08 SS.seek 0ml @16:41:19.07 Exhaust.select :close @16:41:19.17 SSV.dial CBV @16:41:19.20
CBV.dial SSV @16:41:19.94 Allowing 130s for 25.0ml of remaining 650.0ml @16:41:19.95 Intake.select :open @16:41:22.10 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:41:22.12 SS.reconfigure :inhaling 0.30ml/s @16:41:22.16 SS.seek 25ml @16:42:58.64 Stroke completed -- waiting for pressure to equalize ... @16:42:58.65 SS.reconfigure :exhale @16:42:58.69 SS.hold @16:43:04.21 Intake.select :close @16:43:04.35 SSV.dial :exhaust @16:43:05.08 Exhaust.select :open @16:43:05.18 SS.seek 0ml @16:43:14.16 Exhaust.select :close @16:43:14.26 SSV.dial CBV @16:43:14.29
CBV.dial SSV @16:43:15.03 Allowing 130s for 25.0ml of remaining 625.0ml @16:43:15.04 Intake.select :open @16:43:17.19 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:43:17.21 SS.reconfigure :inhaling 0.30ml/s @16:43:17.25 SS.seek 25ml @16:44:55.03 Stroke completed -- waiting for pressure to equalize ... @16:44:55.04 SS.reconfigure :exhale @16:44:55.08 SS.hold @16:45:00.84 Intake.select :close @16:45:00.98 SSV.dial :exhaust @16:45:01.70 Exhaust.select :open @16:45:01.81 SS.seek 0ml @16:45:11.03 Exhaust.select :close @16:45:11.13 SSV.dial CBV @16:45:11.17
CBV.dial SSV @16:45:11.89 Allowing 130s for 25.0ml of remaining 600.0ml @16:45:11.90 Intake.select :open @16:45:14.05 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:45:14.07 SS.reconfigure :inhaling 0.30ml/s @16:45:14.11 SS.seek 25ml @16:46:51.23 Stroke completed -- waiting for pressure to equalize ... @16:46:51.24 SS.reconfigure :exhale @16:46:51.28 SS.hold @16:46:56.80 Intake.select :close @16:46:56.94 SSV.dial :exhaust @16:46:57.67 Exhaust.select :open @16:46:57.78 SS.seek 0ml @16:47:06.96 Exhaust.select :close @16:47:07.06 SSV.dial CBV @16:47:07.09
CBV.dial SSV @16:47:07.82 Allowing 130s for 25.0ml of remaining 575.0ml @16:47:07.83 Intake.select :open @16:47:09.98 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:47:10.00 SS.reconfigure :inhaling 0.30ml/s @16:47:10.04 SS.seek 25ml @16:48:24.11 Vacuum too high -- waiting up to 40 seconds @16:48:24.12 SS.reconfigure :exhale @16:48:24.16 SS.hold @16:48:25.71 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:48:25.72 SS.reconfigure :inhaling 0.27ml/s @16:48:25.77 SS.seek 25ml @16:48:53.33 Stroke completed -- waiting for pressure to equalize ... @16:48:53.34 SS.reconfigure :exhale @16:48:53.38 SS.hold @16:48:58.13 Intake.select :close @16:48:58.27 SSV.dial :exhaust @16:48:58.99 Exhaust.select :open @16:48:59.10 SS.seek 0ml @16:49:08.41 Exhaust.select :close @16:49:08.51 SSV.dial CBV @16:49:08.54
CBV.dial SSV @16:49:09.29 Allowing 130s for 25.0ml of remaining 550.0ml @16:49:09.30 Intake.select :open @16:49:11.45 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:49:11.47 SS.reconfigure :inhaling 0.27ml/s @16:49:11.51 SS.seek 25ml @16:51:00.16 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @16:51:00.20 SS.hold @16:51:04.96 Intake.select :close @16:51:05.11 SSV.dial :exhaust @16:51:05.83 Exhaust.select :open @16:51:05.94 SS.seek 0ml @16:51:14.91 Exhaust.select :close @16:51:15.01 SSV.dial CBV @16:51:15.04
CBV.dial SSV @16:51:15.78 Allowing 130s for 25.0ml of remaining 525.0ml @16:51:15.79 Intake.select :open @16:51:17.94 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:51:17.96 SS.reconfigure :inhaling 0.27ml/s @16:51:18.00 SS.seek 25ml @16:53:09.37 Stroke completed -- waiting for pressure to equalize ... @16:53:09.38 SS.reconfigure :exhale @16:53:09.42 SS.hold @16:53:14.96 Intake.select :close @16:53:15.09 SSV.dial :exhaust @16:53:15.83 Exhaust.select :open @16:53:15.93 SS.seek 0ml @16:53:24.90 Exhaust.select :close @16:53:25.01 SSV.dial CBV @16:53:25.03
CBV.dial SSV @16:53:25.76 Allowing 130s for 25.0ml of remaining 500.0ml @16:53:25.77 Intake.select :open @16:53:27.93 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:53:27.94 SS.reconfigure :inhaling 0.27ml/s @16:53:27.99 SS.seek 25ml @16:55:18.77 Stroke completed -- waiting for pressure to equalize ... @16:55:18.78 SS.reconfigure :exhale @16:55:18.83 SS.hold @16:55:26.27 Intake.select :close @16:55:26.41 SSV.dial :exhaust @16:55:27.13 Exhaust.select :open @16:55:27.24 SS.seek 0ml @16:55:36.62 Exhaust.select :close @16:55:36.72 SSV.dial CBV @16:55:36.76
CBV.dial SSV @16:55:37.48 Allowing 130s for 25.0ml of remaining 475.0ml @16:55:37.49 Intake.select :open @16:55:39.64 Intake @15.3psia, vacuum from 9.8 to 12.8psi @16:55:39.66 SS.reconfigure :inhaling 0.27ml/s @16:55:39.70 SS.seek 25ml @16:57:30.71 Stroke completed -- waiting for pressure to equalize ... @16:57:30.72 SS.reconfigure :exhale @16:57:30.76 SS.hold @16:57:37.24 Intake.select :close @16:57:37.37 SSV.dial :exhaust @16:57:38.10 Exhaust.select :open @16:57:38.20 SS.seek 0ml @16:57:47.64 Exhaust.select :close @16:57:47.75 SSV.dial CBV @16:57:47.77
CBV.dial SSV @16:57:48.50 Allowing 130s for 25.0ml of remaining 450.0ml @16:57:48.52 Intake.select :open @16:57:50.67 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:57:50.68 SS.reconfigure :inhaling 0.27ml/s @16:57:50.72 SS.seek 25ml @16:59:43.09 Stroke completed -- waiting for pressure to equalize ... @16:59:43.10 SS.reconfigure :exhale @16:59:43.14 SS.hold @16:59:51.70 Intake.select :close @16:59:51.83 SSV.dial :exhaust @16:59:52.56 Exhaust.select :open @16:59:52.67 SS.seek 0ml @17:00:01.87 Exhaust.select :close @17:00:01.98 SSV.dial CBV @17:00:02.00
CBV.dial SSV @17:00:02.75 Allowing 130s for 25.0ml of remaining 425.0ml @17:00:02.76 Intake.select :open @17:00:04.91 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:00:04.93 SS.reconfigure :inhaling 0.27ml/s @17:00:04.97 SS.seek 25ml @17:01:28.24 Vacuum too high -- waiting up to 40 seconds @17:01:28.25 SS.reconfigure :exhale @17:01:28.29 SS.hold @17:01:31.81 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:01:31.82 SS.reconfigure :inhaling 0.24ml/s @17:01:31.87 SS.seek 25ml @17:02:04.79 Stroke completed -- waiting for pressure to equalize ... @17:02:04.80 SS.reconfigure :exhale @17:02:05.32 SS.hold @17:02:10.17 Intake.select :close @17:02:10.31 SSV.dial :exhaust @17:02:11.04 Exhaust.select :open @17:02:11.15 SS.seek 0ml @17:02:20.12 Exhaust.select :close @17:02:20.22 SSV.dial CBV @17:02:20.25
CBV.dial SSV @17:02:20.98 Allowing 130s for 25.0ml of remaining 400.0ml @17:02:20.99 Intake.select :open @17:02:23.14 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:02:23.16 SS.reconfigure :inhaling 0.24ml/s @17:02:23.20 SS.seek 25ml @17:04:27.90 Stroke completed -- waiting for pressure to equalize ... @17:04:27.91 SS.reconfigure :exhale @17:04:27.95 SS.hold @17:04:31.00 SS.reconfigure :default @17:04:31.04 SS.hold @17:04:31.14 Intake.select :close @17:04:31.28 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 625.0ml @17:04:31.29 SSV.dial :exhaust @17:04:32.03 Exhaust.select :open @17:04:32.13 SS.reconfigure :exhale @17:04:32.17 SS.seek 0ml @17:04:41.13 Exhaust.select :close @17:04:41.23 Sampled 625.0ml @17:04:41.25 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @17:04:41.31 CSV.dial :waste @17:04:41.35 CS.seek 0ml @17:04:41.45 CSV.dial :air @17:04:41.48 SSV.dial CBV,avoiding: :exhaust @17:04:41.51
CBV.dial SSV @17:04:42.22 CS.seek 10ml @17:04:43.34
CTV.dial :air @17:04:44.08 delay 15 seconds @17:04:59.09 SSV.dial :spit,avoiding: :exhaust @17:05:00.39 CTV.dial CSV,avoiding: :intake @17:05:00.43
SS.seek 0ml @17:05:00.54 SSV.dial CBV,avoiding: :exhaust @17:05:05.16 CBV.dial between SSV and waste2 @17:05:05.53 CTV.dial CSV,avoiding: :air @17:05:05.55
CSV.dial CTV @17:05:06.00 CS.reconfigure :pressurize @17:05:06.05 CS.seek 0ml @17:05:24.73 BubblePoint: Intake @40.5psia, Outlet @16.3psia @17:05:24.74 CBV.dial SSV @17:05:25.05 SS.reconfigure :default @17:05:25.09 SS.seek 10ml @17:05:30.76 delay 15 seconds @17:05:45.87 Evacuated: Intake @29.4psia, Outlet @2.0psia @17:05:45.88 SSV.dial :spit,avoiding: :exhaust @17:05:47.18 SS.reconfigure :exhale @17:05:47.23 SS.seek 0ml @17:05:52.53 SS.reconfigure :fast @17:05:52.57 SS.seek 5ml @17:05:55.40 SSV.dial CBV,avoiding: :exhaust @17:05:55.43
CBV.dial SSV @17:05:56.70 CTV.dial :air,avoiding: :intake @17:05:57.97 CSV.dial CTV @17:05:58.00
SS.seek 10ml @17:05:59.00 CSV.dial :air @17:05:59.45 CS.reconfigure :fast @17:05:59.50 CS.seek 0ml @17:06:00.71
SSV.dial :spit,avoiding: :exhaust @17:06:02.01 SS.reconfigure :exhale @17:06:02.05 SS.seek 0ml @17:06:06.44 CS.stop @17:06:07.58
SS.reconfigure :fast @17:06:07.62 SS.seek 5ml @17:06:10.23 SSV.dial CBV,avoiding: :exhaust @17:06:10.25
CBV.dial SSV @17:06:12.55 SSV.dial :spit,avoiding: :exhaust @17:06:13.84 SS.reconfigure :default @17:06:13.88 SS.seek 0ml @17:06:17.19 SS.stop @17:06:17.23 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @17:06:17.30 CSV.dial :waste @17:06:17.41 SSV.dial CBV,avoiding: :exhaust @17:06:17.44
CBV.dial SSV @17:06:18.05 CS.seek 0ml @17:06:18.11 CSV.dial :air @17:06:18.83 CS.seek 10ml @17:06:19.15
CTV.dial :air @17:06:19.16 delay 15 seconds @17:06:34.18 SSV.dial :spit,avoiding: :exhaust @17:06:35.46 CTV.dial CSV,avoiding: :intake @17:06:35.50
SS.reconfigure :exhale @17:06:35.58 SS.seek 0ml @17:06:35.64 SSV.dial CBV,avoiding: :exhaust @17:06:41.47 CBV.dial between SSV and waste2 @17:06:41.85 CSV.dial between air and CTV @17:06:42.14 CS.seek 8.23ml @17:06:46.92 CTV.dial CSV,avoiding: :air @17:06:46.94
CSV.dial CTV @17:06:47.25 CS.reconfigure :pressurize @17:06:47.29 CS.seek 0ml @17:07:03.54 BubblePoint: Intake @39.9psia, Outlet @15.8psia @17:07:03.55 CBV.dial SSV @17:07:03.86 SS.reconfigure :default @17:07:03.90 SS.seek 10ml @17:07:09.78 delay 15 seconds @17:07:24.90 Evacuated: Intake @39.3psia, Outlet @2.6psia @17:07:24.91 SSV.dial :spit,avoiding: :exhaust @17:07:26.19 SS.reconfigure :exhale @17:07:26.24 SS.seek 0ml @17:07:31.51 SS.reconfigure :fast @17:07:31.55 SS.seek 5ml @17:07:34.55 SSV.dial CBV,avoiding: :exhaust @17:07:34.58
CBV.dial SSV @17:07:35.87 CTV.dial :air,avoiding: :intake @17:07:37.15 CSV.dial CTV @17:07:37.19
SS.seek 10ml @17:07:38.18 CSV.dial :air @17:07:38.64 CS.reconfigure :fast @17:07:38.68 CS.seek 0ml @17:07:39.86
SSV.dial :spit,avoiding: :exhaust @17:07:41.15 SS.reconfigure :exhale @17:07:41.20 SS.seek 0ml @17:07:43.82 CS.stop @17:07:46.42
SS.reconfigure :fast @17:07:46.46 SS.seek 5ml @17:07:49.24 SSV.dial CBV,avoiding: :exhaust @17:07:49.27
CBV.dial SSV @17:07:51.56 SSV.dial :spit,avoiding: :exhaust @17:07:52.85 SS.reconfigure :default @17:07:52.89 SS.seek 0ml @17:07:56.22 SS.stop @17:07:56.25 Lysing: Adding 2.0ml AqMeOH @17:07:56.26 CBV.dial between CSV and waste1,avoiding: :DBV @17:07:57.70 CTV.dial CSV @17:07:58.96 CSV.dial CSR @17:07:59.73 Pulling 1ml of CSR.AqMeOH @17:07:59.74 CSR.select :AqMeOH @17:07:59.85 CS.reconfigure :default @17:07:59.89 CS.seek 1ml @17:08:03.29 delay 5 seconds @17:08:08.31 CSR.select :air @17:08:08.46 CS.seek 6ml @17:08:21.82 delay 2 seconds @17:08:23.83 CSV.dial :waste2 @17:08:24.26 CS.reconfigure :fast @17:08:24.30 CS.seek 0ml @17:08:38.13 CSV.dial CSR @17:08:38.62 Pulling 2ml of CSR.AqMeOH @17:08:38.63 CSR.select :AqMeOH @17:08:38.74 CS.reconfigure :default @17:08:38.78 CS.seek 2ml @17:08:44.60 delay 5 seconds @17:08:49.62 CSR.select :air @17:08:49.77 CS.seek 5ml @17:08:58.19 delay 2 seconds @17:09:00.21 CSV.dial CTV @17:09:00.64 CS.seek 1ml @17:09:11.67 Before lysing Collection Puck Heater at 17.5C/42.5psia @17:09:11.69 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @17:09:11.70 CH.seek 70C,8:00 @17:10:57.89 CH.hold 10:00,70C @17:10:58.05 Begun lysing Collection Puck Heater at 68C/46.6psia @17:20:58.07 Ended lysing Collection Puck Heater at 70C/48.1psia @17:20:58.09 CS.seek 0ml @17:21:01.62 CTV.dial between spare and air,avoiding: :intake @17:21:02.60 CSV.dial :air @17:21:03.04 CS.seek 5ml @17:21:16.58 CSV.dial CTV @17:21:17.02 delay 2 seconds @17:21:19.04 CSV.dial :air @17:21:19.49 delay 2 seconds @17:21:21.50 CSV.dial CTV @17:21:21.96 delay 2 seconds @17:21:23.97 CSV.dial :air @17:21:24.42 delay 2 seconds @17:21:26.45 CS.seek 0ml @17:21:39.97 Puck @77.9psia after final lysis pressurization @17:21:39.99 Recovering Lysate into Collection syringe CSV.dial CBV @17:21:41.09 CBV.dial CSV @17:21:41.45 CS.seek 5ml @17:21:54.86 delay 5 seconds @17:21:59.88 CTV.dial :air @17:22:00.39 CS.seek 7ml @17:22:06.20 delay 5 seconds @17:22:11.22 CSV.dial :air2 @17:22:11.68 CS.seek 10ml @17:22:20.14 CSV.dial between air and CTV,avoiding: CBV @17:22:21.25 Lysing Complete @17:22:21.32 Storing sh1 puck in Collection Clamp to tube 2 @17:22:21.48 SC.reconfigure :tight @17:22:21.52 SC.seek 2 @17:22:25.02 SC.configure :limp @17:22:25.21 Elbow.reconfigure :tight @17:22:25.26 Elbow.seek 2 @17:22:27.10 Forearm.reconfigure :tight @17:22:27.14 Forearm.seek 2 + 700 counts @17:22:28.28 SE.reconfigure :pick @17:22:28.33 SE.seek :beamBreak @17:22:31.33 SE.configure :default @17:22:31.47 Elbow.reconfigure :limp @17:22:31.52 Forearm.reconfigure :loose @17:22:31.56 Forearm.seek 2 @17:22:31.98 Elbow.hold @17:22:32.04 Elbow.reconfigure :tight @17:22:32.13 SE.seek 0.18 Pucks below Top Plate @17:22:32.58 Forearm.force -20 @17:22:32.61 Forearm.seek 2 + 315 counts @17:22:32.99 Forearm.reconfigure :tight @17:22:33.03 Forearm.seek 2 + 700 counts @17:22:33.41 SE.seek 1.18 Pucks below Top Plate @17:22:33.87 Forearm.reconfigure :loose @17:22:33.91 Forearm.seek :retract @17:22:34.85 Elbow.seek CC @17:22:36.36 Elbow.reconfigure :loose @17:22:36.45 Forearm.seek CC @17:22:37.44 Hand.close @17:22:38.61 CC.seek :open @17:22:43.04 Forearm.seek :retract @17:22:44.11 Elbow.reconfigure :tight @17:22:44.15 Elbow.seek 2 @17:22:46.01 Elbow.reconfigure :limp @17:22:46.06 Forearm.seek 2 @17:22:47.14 Elbow.hold @17:22:47.20 Elbow.reconfigure :tight @17:22:47.24 Hand.open @17:22:48.10 Forearm.force -20 @17:22:48.13 Forearm.seek 2 + 314 counts @17:22:48.44 Forearm.reconfigure :tight @17:22:48.48 Forearm.seek 2 + 700 counts @17:22:48.84 SE.seek :down @17:22:49.97
Forearm.reconfigure :loose @17:22:50.01 Forearm.seek :retract @17:22:51.45 SE.stop @17:22:51.49
Tranferring DA @17:22:51.50 Beginning samplePassC @17:22:51.51 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:22:51.69 Elbow.seek FlushPuck::Garage @17:22:53.71 Elbow.reconfigure :loose @17:22:53.80 Forearm.seek :garage @17:22:54.90 Hand.close @17:22:55.59 Forearm.seek :retract @17:22:56.74 Elbow.reconfigure :tight @17:22:56.78 Elbow.seek CC @17:22:59.65 Elbow.reconfigure :loose @17:22:59.74 Forearm.reconfigure :tight @17:22:59.78 Forearm.seek CC @17:23:01.01 CC.seek :closed @17:23:05.27 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.89 @17:23:05.33 Hand.open @17:23:06.17 Forearm.reconfigure :loose @17:23:06.21 Forearm.seek :retract @17:23:07.20 PSV.dial PSR @17:23:08.51 Pulling 1.5ml of PSR.flush @17:23:08.52 PSR.select :flush @17:23:08.63 PS.seek 1.5ml @17:23:24.60 delay 5 seconds @17:23:29.62 PSV.dial :waste1 @17:23:30.05 PS.seek 0ml @17:23:46.12 PSV.dial PSR @17:23:46.67 PSR.select :air @17:23:46.79 PS.seek 2.5ml @17:24:12.94 PRV.dial CBV,avoiding: :ab2 @17:24:12.98
CBV.dial PRV @17:24:14.58 PSV.dial :mixloop @17:24:15.56 CTV.dial :air,avoiding: :intake @17:24:15.58 PTV.dial :PRVmixing @17:24:16.80 PS.seek 0ml @17:24:42.94 PSV.dial :air @17:24:43.36 PS.seek 2.5ml @17:25:09.56 PSV.dial :mixloop @17:25:10.00 PS.seek 0ml @17:25:36.12 PSV.dial :air @17:25:36.57 PS.seek 2.5ml @17:26:02.71 PSV.dial :mixloop @17:26:03.16 PS.seek 0ml @17:26:29.34 Garaging FlushPuck in Collection Clamp @17:26:29.41 Elbow.reconfigure :tight @17:26:29.45 Elbow.seek CC @17:26:29.56 Elbow.reconfigure :loose @17:26:29.65 Forearm.seek CC @17:26:30.62 Hand.close @17:26:31.73 CC.seek :open @17:26:36.09 Forearm.seek :retract @17:26:37.10 Elbow.reconfigure :tight @17:26:37.15 Elbow.seek :garage @17:26:40.09 Elbow.reconfigure :loose @17:26:40.18 Forearm.reconfigure :tight @17:26:40.23 Forearm.seek :garage @17:26:41.32 Hand.open @17:26:42.16 Forearm.reconfigure :loose @17:26:42.20 Forearm.seek :retract @17:26:43.28 PSV.dial :mixloop @17:26:43.29 PTV.dial :PRVmixing @17:26:43.30 PRV.dial CBV @17:26:43.32
CBV.dial PRV @17:26:43.34 CTV.dial :air,avoiding: :intake @17:26:43.36 Loading lyfil puck from tube 3 into Collection Clamp @17:26:43.62 SC.reconfigure :tight @17:26:43.66 SC.seek 3 @17:26:47.26 SC.configure :limp @17:26:47.45 Elbow.reconfigure :tight @17:26:47.50 Elbow.seek 3 @17:26:49.52 Forearm.reconfigure :tight @17:26:49.57 Forearm.seek 3 + 700 counts @17:26:50.63 SE.reconfigure :pick @17:26:50.68 SE.seek :beamBreak @17:26:51.69 SE.configure :default @17:26:51.84 Elbow.seek 3 @17:26:52.26 Forearm.seek 3 + 300 counts @17:26:52.52 Forearm.reconfigure :loose @17:26:52.57 Hand.close @17:26:53.24 SE.seek :down @17:26:53.43
Forearm.seek :retract @17:26:54.01 SE.stop @17:26:54.50
Elbow.seek CC @17:26:56.11 Elbow.reconfigure :loose @17:26:56.20 Forearm.reconfigure :tight @17:26:56.24 Forearm.seek CC @17:26:57.52 CC.seek :closed @17:27:01.74 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.72 @17:27:01.75 Hand.open @17:27:02.54 Forearm.reconfigure :loose @17:27:02.58 Forearm.seek :retract @17:27:03.63 CTV.dial CSV,avoiding: :intake @17:27:04.90 CSV.dial CTV @17:27:05.20 PS.seek 2.5ml @17:27:31.43 CS.seek 5.501ml @17:27:43.51 delay 8 seconds @17:27:51.53 PSV.dial :air @17:27:51.98 delay 2 seconds @17:27:53.99 PSV.dial :waste2 @17:27:54.70 PS.seek 0ml @17:28:20.93 CBV.dial between PRV and waste1 @17:28:21.26 CSV.dial :air @17:28:21.71 CS.seek 10ml @17:28:33.88 CSV.dial CTV @17:28:34.31 CS.seek 0ml @17:29:00.35 CBV.dial :waste1 @17:29:00.65 delay 5 seconds @17:29:05.67 CTV.dial :air,avoiding: :intake @17:29:06.97 Storing lyfil puck in Collection Clamp to tube 2 @17:29:07.13 SC.reconfigure :tight @17:29:07.17 SC.seek 2 @17:29:10.76 SC.configure :limp @17:29:11.42 Elbow.reconfigure :tight @17:29:11.47 Elbow.seek 2 @17:29:13.21 Forearm.reconfigure :tight @17:29:13.25 Forearm.seek 2 + 700 counts @17:29:14.30 SE.reconfigure :pick @17:29:14.35 SE.seek :beamBreak @17:29:17.37 SE.configure :default @17:29:17.51 Elbow.reconfigure :limp @17:29:17.56 Forearm.reconfigure :loose @17:29:17.60 Forearm.seek 2 @17:29:17.97 Elbow.hold @17:29:18.03 Elbow.reconfigure :tight @17:29:18.12 SE.seek 1.18 Pucks below Top Plate @17:29:18.54 Forearm.force -20 @17:29:18.58 Forearm.seek 2 + 316 counts @17:29:18.87 Forearm.reconfigure :tight @17:29:18.91 Forearm.seek 2 + 700 counts @17:29:19.24 SE.seek 2.18 Pucks below Top Plate @17:29:19.72 Forearm.reconfigure :loose @17:29:19.77 Forearm.seek :retract @17:29:20.85 Elbow.seek CC @17:29:22.50 Elbow.reconfigure :loose @17:29:22.59 Forearm.seek CC @17:29:23.50 Hand.close @17:29:24.22 CC.seek :open @17:29:28.65 Forearm.seek :retract @17:29:29.61 Elbow.reconfigure :tight @17:29:29.66 Elbow.seek 2 @17:29:31.56 Elbow.reconfigure :limp @17:29:31.61 Forearm.seek 2 @17:29:32.72 Elbow.hold @17:29:32.78 Elbow.reconfigure :tight @17:29:32.82 Hand.open @17:29:33.71 Forearm.force -20 @17:29:33.75 Forearm.seek 2 + 318 counts @17:29:34.12 Forearm.reconfigure :tight @17:29:34.16 Forearm.seek 2 + 700 counts @17:29:34.57 SE.seek :down @17:29:35.70
Forearm.reconfigure :loose @17:29:35.74 Forearm.seek :retract @17:29:37.13 SE.stop @17:29:37.17
PSV.dial :air @17:29:37.89 PS.seek 2.5ml @17:30:04.09 delay 1 second @17:30:05.10 PSV.dial :mixloop @17:30:05.52 delay 1 second @17:30:06.54 PSV.dial :air @17:30:06.98 delay 1 second @17:30:07.99 PSV.dial :mixloop @17:30:08.43 delay 1 second @17:30:09.44 PSV.dial :air @17:30:09.90 delay 1 second @17:30:10.92 PS.seek 0ml @17:30:37.05 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:30:37.22 Elbow.seek FlushPuck::Garage @17:30:39.27 Elbow.reconfigure :loose @17:30:39.36 Forearm.seek :garage @17:30:40.47 Hand.close @17:30:41.20 Forearm.seek :retract @17:30:42.28 Elbow.reconfigure :tight @17:30:42.33 Elbow.seek PC @17:30:43.65 Elbow.reconfigure :loose @17:30:43.74 Forearm.reconfigure :tight @17:30:43.78 Forearm.seek PC @17:30:45.11 PC.seek :closed @17:30:48.99 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.42 @17:30:49.05 Hand.open @17:30:49.90 Forearm.reconfigure :loose @17:30:49.95 Forearm.seek :retract @17:30:50.95 PRV.dial :air @17:30:51.39 PSV.dial :mixloop @17:30:51.90 PS.seek 0.3ml @17:30:55.83 PSV.dial PSR @17:30:56.85 Pulling 0.4ml of PSR.air @17:30:56.86 PSR.select :air @17:30:56.89 PS.seek 0.7ml @17:31:01.77 delay 3 seconds @17:31:04.79 PSR.select :air @17:31:04.81 PS.seek 2.5ml @17:31:23.80 delay 3 seconds @17:31:26.82 PSV.dial :mixloop @17:31:27.80 PTV.dial :mixing @17:31:28.52 PBV.dial :waste2 @17:31:29.39 PS.seek 0ml @17:31:55.52 Garaging FlushPuck in Processing Clamp @17:31:55.59 Elbow.reconfigure :tight @17:31:55.63 Elbow.seek PC @17:31:55.74 Elbow.reconfigure :loose @17:31:55.82 Forearm.seek PC @17:31:56.78 Hand.close @17:31:57.34 PC.seek :open @17:32:01.29 Forearm.seek :retract @17:32:02.27 Elbow.reconfigure :tight @17:32:02.31 Elbow.seek :garage @17:32:03.66 Elbow.reconfigure :loose @17:32:03.75 Forearm.reconfigure :tight @17:32:03.79 Forearm.seek :garage @17:32:04.90 Hand.open @17:32:05.65 Forearm.reconfigure :loose @17:32:05.69 Forearm.seek :retract @17:32:06.74 Loading da puck from tube 3 into Processing Clamp @17:32:06.96 SC.reconfigure :tight @17:32:07.00 SC.seek 3 @17:32:10.54 SC.configure :limp @17:32:10.73 Elbow.reconfigure :tight @17:32:10.77 Elbow.seek 3 @17:32:12.87 Forearm.reconfigure :tight @17:32:12.92 Forearm.seek 3 + 700 counts @17:32:13.90 SE.reconfigure :pick @17:32:13.95 SE.seek :beamBreak @17:32:15.09 SE.configure :default @17:32:15.23 Elbow.seek 3 @17:32:15.45 Forearm.seek 3 + 300 counts @17:32:15.62 Forearm.reconfigure :loose @17:32:15.66 Hand.close @17:32:16.23 SE.seek :down @17:32:16.42
Forearm.seek :retract @17:32:16.97 SE.stop @17:32:17.39
Elbow.seek PC @17:32:20.03 Elbow.reconfigure :loose @17:32:20.13 Forearm.reconfigure :tight @17:32:20.17 Forearm.seek PC @17:32:21.52 PC.seek :closed @17:32:25.40 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.31 Hand.open @17:32:26.15 Forearm.reconfigure :loose @17:32:26.19 Forearm.seek :retract @17:32:27.29 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @17:32:27.30 PH.seek 28C,5:00 @17:33:03.43 PH.hold 2:00:00,28C @17:33:03.49 delay 1:30 @17:34:33.52 PSR.select :air @17:34:33.53 PSV.dial PSR @17:34:34.58 Pulling 0.5ml of PSR.wash @17:34:34.59 PSR.select :wash @17:34:34.71 PS.seek 0.5ml @17:34:40.61 delay 5 seconds @17:34:45.63 PSR.select :air @17:34:45.74 PS.seek 2ml @17:35:01.86 PTV.dial :puck @17:35:02.55 PSV.dial PTV @17:35:03.26 PBV.dial :waste1 @17:35:04.56 PS.seek 1.5ml @17:35:10.45 delay 3 seconds @17:35:13.47 PBV.dial between PSV and waste1 @17:35:13.84 PS.seek 0ml @17:35:29.89 delay 5:00 @17:40:29.91 recoverPSRreagent08 @17:40:29.92 PTV.dial :puck @17:40:29.93 PBV.dial :waste1 @17:40:30.29 delay 30 seconds @17:41:00.31 PBV.dial between PSV and waste1 @17:41:00.68 PSR.select :air @17:41:00.69 PSV.dial PSR @17:41:01.42 PS.reconfigure :fast @17:41:01.46 PS.seek 2.5ml @17:41:24.07 PSV.dial PTV @17:41:24.77 delay 5 seconds @17:41:29.79 PSV.dial PSR @17:41:30.51 delay 5 seconds @17:41:35.52 PSV.dial PTV @17:41:36.24 delay 5 seconds @17:41:41.26 PSV.dial :air @17:41:41.98 PS.seek 0ml @17:42:04.32 PTV.dial :PRVmixing,via: PRV @17:42:05.57 PSV.dial :mixloop @17:42:06.02 PRV.dial :ab1 @17:42:06.74 PS.reconfigure :slow @17:42:06.79 PS.seek 0.15ml @17:42:10.35 delay 2 seconds @17:42:12.37 CBV.dial PRV @17:42:12.39
PRV.dial CBV @17:42:12.96 PS.seek 0.34ml @17:42:17.25 delay 2 seconds @17:42:19.27 CBV.dial between PRV and PSV @17:42:19.58 PRV.dial :air @17:42:20.01 PS.seek 0.9ml @17:42:31.81 PRV.dial :waste2 @17:42:32.52 PS.seek 0.5ml @17:42:41.22 addPMLReagent08 @17:42:41.23 PSV.dial :air @17:42:41.68 PS.reconfigure :default @17:42:41.72 PS.seek 2ml @17:42:57.75 PSV.dial :PML @17:42:58.19 PTV.dial :mixing @17:42:58.89 PBV.dial :waste1 @17:42:59.29 PS.seek 1.85ml @17:43:01.66 PBV.dial between PSV and waste1 @17:43:02.03 PS.seek 0ml @17:43:21.66 delay 2 seconds @17:43:23.67 Beginning samplePassC @17:43:23.68 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:43:23.84 Elbow.reconfigure :tight @17:43:23.88 Elbow.seek FlushPuck::Garage @17:43:25.26 Elbow.reconfigure :loose @17:43:25.35 Forearm.seek :garage @17:43:26.47 Hand.close @17:43:27.18 Forearm.seek :retract @17:43:28.35 Elbow.reconfigure :tight @17:43:28.40 Elbow.seek CC @17:43:31.31 Elbow.reconfigure :loose @17:43:31.40 Forearm.reconfigure :tight @17:43:31.44 Forearm.seek CC @17:43:33.23 CC.seek :closed @17:43:37.46 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.84 @17:43:37.54 Hand.open @17:43:38.25 Forearm.reconfigure :loose @17:43:38.30 Forearm.seek :retract @17:43:39.40 PSV.dial PSR @17:43:40.43 Pulling 1.5ml of PSR.flush @17:43:40.44 PSR.select :flush @17:43:40.56 PS.seek 1.5ml @17:43:56.63 delay 5 seconds @17:44:01.65 PSV.dial :waste1 @17:44:02.08 PS.seek 0ml @17:44:18.15 PSV.dial PSR @17:44:18.59 PSR.select :air @17:44:18.70 PS.seek 2.5ml @17:44:44.88 PRV.dial CBV,avoiding: :ab2 @17:44:44.91
CBV.dial PRV @17:44:46.45 PSV.dial :mixloop @17:44:47.44 CTV.dial :air,avoiding: :intake @17:44:47.45 PTV.dial :PRVmixing @17:44:48.18 PS.seek 0ml @17:45:14.40 PSV.dial :air @17:45:14.83 PS.seek 2.5ml @17:45:41.06 PSV.dial :mixloop @17:45:41.50 PS.seek 0ml @17:46:07.81 PSV.dial :air @17:46:08.25 PS.seek 2.5ml @17:46:34.49 PSV.dial :mixloop @17:46:34.94 PS.seek 0ml @17:47:01.15 Garaging FlushPuck in Collection Clamp @17:47:01.22 Elbow.reconfigure :tight @17:47:01.26 Elbow.seek CC @17:47:01.47 Elbow.reconfigure :loose @17:47:01.57 Forearm.seek CC @17:47:02.61 Hand.close @17:47:03.31 CC.seek :open @17:47:07.65 Forearm.seek :retract @17:47:08.66 Elbow.reconfigure :tight @17:47:08.70 Elbow.seek :garage @17:47:11.54 Elbow.reconfigure :loose @17:47:11.63 Forearm.reconfigure :tight @17:47:11.67 Forearm.seek :garage @17:47:12.79 Hand.open @17:47:13.62 Forearm.reconfigure :loose @17:47:13.66 Forearm.seek :retract @17:47:14.78 delayUntil "17:51:23.66" @17:47:14.81 Whole Cell Archive sampling 1000ml @17:47:14.82 Loading wcr puck from tube 3 into Collection Clamp @17:47:15.04 SC.reconfigure :tight @17:47:15.09 SC.seek 3 @17:47:15.19 SC.configure :limp @17:47:15.39 Elbow.reconfigure :tight @17:47:15.44 Elbow.seek 3 @17:47:17.41 Forearm.reconfigure :tight @17:47:17.46 Forearm.seek 3 + 700 counts @17:47:18.50 SE.reconfigure :pick @17:47:18.56 SE.seek :beamBreak @17:47:19.73 SE.configure :default @17:47:19.87 Elbow.seek 3 @17:47:20.04 Forearm.seek 3 + 300 counts @17:47:20.31 Forearm.reconfigure :loose @17:47:20.36 Hand.close @17:47:20.95 SE.seek :down @17:47:21.13 Forearm.seek :retract @17:47:21.77 SE.stop @17:47:22.56 Elbow.seek CC @17:47:24.09 Elbow.reconfigure :loose @17:47:24.18 Forearm.reconfigure :tight @17:47:24.23 Forearm.seek CC @17:47:25.52 CC.seek :closed @17:47:29.75 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.66 @17:47:29.76 Hand.open @17:47:30.50 Forearm.reconfigure :loose @17:47:30.54 Forearm.seek :retract @17:47:31.63 collectSample 1000ml w/bubble point of 35psi @17:47:31.64 Priming 7ml @17:47:31.65 Intake.select :close @17:47:31.66 Exhaust.select :close @17:47:31.67 SS.hold @17:47:31.87 Calibrating pressure sensors @17:47:31.88 CTV.dial :air @17:47:31.89 SSV.dial :spit,avoiding: :exhaust @17:47:31.91 SS.reconfigure :fast @17:47:31.95 SS.seek 5ml @17:47:34.69 SSV.dial CBV @17:47:34.74 CBV.dial SSV @17:47:35.99 delay 3.8 seconds @17:47:39.91 SSV.dial :spit @17:47:41.20 SS.seek 0ml @17:47:43.96 SSV.dial CBV @17:47:45.23 CTV.dial :intake @17:47:45.96 SSV.dial CBV @17:47:45.99 CBV.dial SSV @17:47:46.01 Allowing 40s for 7.0ml of remaining 7.0ml @17:47:46.02 Intake.select :open @17:47:48.17 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:47:48.19 SS.reconfigure :inhaling 2.14ml/s @17:47:48.24 SS.seek 7ml @17:47:54.67 Stroke completed -- waiting for pressure to equalize ... @17:47:54.69 SS.reconfigure :exhale @17:47:54.73 SS.hold @17:47:58.80 Intake.select :close @17:47:58.95 SSV.dial :exhaust @17:47:59.68 Exhaust.select :open @17:47:59.79 SS.seek 0ml @17:48:03.77 Exhaust.select :close @17:48:03.87 Sampling 1000.0ml @17:48:03.89 Intake.select :close @17:48:03.90 Exhaust.select :close @17:48:03.91 SS.reconfigure :default @17:48:03.95 SS.hold @17:48:04.14 CTV.dial :intake @17:48:04.16 SSV.dial CBV @17:48:04.19 CBV.dial SSV @17:48:04.93 Allowing 130s for 25.0ml of remaining 1000.0ml @17:48:04.94 Intake.select :open @17:48:07.09 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:48:07.11 SS.reconfigure :inhaling 2.14ml/s @17:48:07.15 SS.seek 25ml @17:48:12.65 Vacuum too high -- waiting up to 40 seconds @17:48:12.66 SS.reconfigure :exhale @17:48:12.70 SS.hold @17:48:13.38 Intake @11.5psia, vacuum from 6.0 to 9.0psi @17:48:13.39 SS.reconfigure :inhaling 1.93ml/s @17:48:13.44 SS.seek 25ml @17:48:16.59 Vacuum too high -- waiting up to 40 seconds @17:48:16.60 SS.reconfigure :exhale @17:48:16.64 SS.hold @17:48:17.82 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:48:17.84 SS.reconfigure :inhaling 1.74ml/s @17:48:17.88 SS.seek 25ml @17:48:21.43 Vacuum too high -- waiting up to 40 seconds @17:48:21.44 SS.reconfigure :exhale @17:48:21.49 SS.hold @17:48:22.54 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:48:22.56 SS.reconfigure :inhaling 1.57ml/s @17:48:22.60 SS.seek 25ml @17:48:29.37 Vacuum too high -- waiting up to 40 seconds @17:48:29.38 SS.reconfigure :exhale @17:48:29.43 SS.hold @17:48:29.91 Intake @11.7psia, vacuum from 6.2 to 9.2psi @17:48:29.93 SS.reconfigure :inhaling 1.41ml/s @17:48:29.97 SS.seek 25ml @17:48:32.92 Stroke completed -- waiting for pressure to equalize ... @17:48:32.93 SS.reconfigure :exhale @17:48:32.98 SS.hold @17:48:33.54 Intake.select :close @17:48:33.68 SSV.dial :exhaust @17:48:34.41 Exhaust.select :open @17:48:34.53 SS.seek 0ml @17:48:43.33 Exhaust.select :close @17:48:43.44 SSV.dial CBV @17:48:43.47 CBV.dial SSV @17:48:44.21 Allowing 130s for 25.0ml of remaining 975.0ml @17:48:44.22 Intake.select :open @17:48:46.37 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:48:46.39 SS.reconfigure :inhaling 1.41ml/s @17:48:46.44 SS.seek 25ml @17:49:00.27 Vacuum too high -- waiting up to 40 seconds @17:49:00.29 SS.reconfigure :exhale @17:49:00.33 SS.hold @17:49:00.79 Intake @9.8psia, vacuum from 4.3 to 7.3psi @17:49:00.81 SS.reconfigure :inhaling 1.27ml/s @17:49:00.85 SS.seek 25ml @17:49:08.45 Stroke completed -- waiting for pressure to equalize ... @17:49:08.46 SS.reconfigure :exhale @17:49:08.51 SS.hold @17:49:09.36 Intake.select :close @17:49:09.50 SSV.dial :exhaust @17:49:10.24 Exhaust.select :open @17:49:10.35 SS.seek 0ml @17:49:19.50 Exhaust.select :close @17:49:19.61 SSV.dial CBV @17:49:19.64 CBV.dial SSV @17:49:20.38 Allowing 130s for 25.0ml of remaining 950.0ml @17:49:20.39 Intake.select :open @17:49:22.55 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:49:22.57 SS.reconfigure :inhaling 1.27ml/s @17:49:22.61 SS.seek 25ml @17:49:44.08 Stroke completed -- waiting for pressure to equalize ... @17:49:44.09 SS.reconfigure :exhale @17:49:44.13 SS.hold @17:49:45.31 Intake.select :close @17:49:45.45 SSV.dial :exhaust @17:49:46.19 Exhaust.select :open @17:49:46.30 SS.seek 0ml @17:49:55.41 Exhaust.select :close @17:49:55.51 SSV.dial CBV @17:49:55.54 CBV.dial SSV @17:49:56.30 Allowing 130s for 25.0ml of remaining 925.0ml @17:49:56.31 Intake.select :open @17:49:58.47 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:49:58.48 SS.reconfigure :inhaling 1.27ml/s @17:49:58.53 SS.seek 25ml @17:50:19.87 Stroke completed -- waiting for pressure to equalize ... @17:50:19.88 SS.reconfigure :exhale @17:50:19.93 SS.hold @17:50:21.21 Intake.select :close @17:50:21.36 SSV.dial :exhaust @17:50:22.09 Exhaust.select :open @17:50:22.20 SS.seek 0ml @17:50:31.01 Exhaust.select :close @17:50:31.12 SSV.dial CBV @17:50:31.15 CBV.dial SSV @17:50:31.89 Allowing 130s for 25.0ml of remaining 900.0ml @17:50:31.90 Intake.select :open @17:50:34.06 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:50:34.07 SS.reconfigure :inhaling 1.27ml/s @17:50:34.12 SS.seek 25ml @17:50:56.40 Stroke completed -- waiting for pressure to equalize ... @17:50:56.41 SS.reconfigure :exhale @17:50:56.46 SS.hold @17:50:57.41 Intake.select :close @17:50:57.55 SSV.dial :exhaust @17:50:58.28 Exhaust.select :open @17:50:58.97 SS.seek 0ml @17:51:08.23 Exhaust.select :close @17:51:08.34 SSV.dial CBV @17:51:08.37 CBV.dial SSV @17:51:09.12 Allowing 130s for 25.0ml of remaining 875.0ml @17:51:09.14 Intake.select :open @17:51:11.29 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:51:11.31 SS.reconfigure :inhaling 1.27ml/s @17:51:11.36 SS.seek 25ml @17:51:23.68 recoverPRVreagent08 @17:51:23.69 PTV.dial :mixing @17:51:24.41 PBV.dial :waste1 @17:51:24.77 delay 30 seconds @17:51:32.70 Stroke completed -- waiting for pressure to equalize ... @17:51:32.71 SS.reconfigure :exhale @17:51:32.75 SS.hold @17:51:33.95 Intake.select :close @17:51:34.09 SSV.dial :exhaust @17:51:34.83 Exhaust.select :open @17:51:34.94 SS.seek 0ml @17:51:43.79 Exhaust.select :close @17:51:43.90 SSV.dial CBV @17:51:43.94 CBV.dial SSV @17:51:44.65 Allowing 130s for 25.0ml of remaining 850.0ml @17:51:44.67 Intake.select :open @17:51:46.82 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:51:46.84 SS.reconfigure :inhaling 1.27ml/s @17:51:46.88 SS.seek 25ml @17:51:54.79 PBV.dial between PSV and waste1 @17:51:55.14 PSV.dial :air @17:51:55.60 PS.reconfigure :fast @17:51:55.65 PS.seek 2.5ml @17:52:09.17 Stroke completed -- waiting for pressure to equalize ... @17:52:09.18 SS.reconfigure :exhale @17:52:09.22 SS.hold @17:52:10.41 Intake.select :close @17:52:10.55 SSV.dial :exhaust @17:52:11.28 Exhaust.select :open @17:52:11.39 SS.seek 0ml @17:52:18.23 PSV.dial :mixloop @17:52:18.69 delay 2 seconds @17:52:20.26 Exhaust.select :close @17:52:20.37 SSV.dial CBV @17:52:20.39 CBV.dial SSV @17:52:20.70 PSV.dial :air @17:52:21.15 Allowing 130s for 25.0ml of remaining 825.0ml @17:52:21.16 Intake.select :open @17:52:21.22 delay 2 seconds @17:52:23.23 PSV.dial :mixloop @17:52:23.32 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:52:23.34 SS.reconfigure :inhaling 1.27ml/s @17:52:23.39 SS.seek 25ml @17:52:23.69 delay 2 seconds @17:52:25.71 PSV.dial :air @17:52:26.16 PS.seek 0ml @17:52:45.68 Stroke completed -- waiting for pressure to equalize ... @17:52:45.69 SS.reconfigure :exhale @17:52:45.74 SS.hold @17:52:47.28 Intake.select :close @17:52:47.43 SSV.dial :exhaust @17:52:48.15 Exhaust.select :open @17:52:48.27 SS.seek 0ml @17:52:48.61 washCycleDA #1 of 2 @17:52:48.62 PSR.select :air @17:52:48.64 PSV.dial PSR @17:52:49.94 Pulling 0.5ml of PSR.wash @17:52:49.95 PSR.select :wash @17:52:50.07 PS.reconfigure :default @17:52:50.11 PS.seek 0.5ml @17:52:56.08 delay 5 seconds @17:52:57.65 Exhaust.select :close @17:52:57.76 SSV.dial CBV @17:52:57.79 CBV.dial SSV @17:52:58.53 Allowing 130s for 25.0ml of remaining 800.0ml @17:52:58.54 Intake.select :open @17:53:00.69 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:53:00.71 SS.reconfigure :inhaling 1.27ml/s @17:53:00.76 SS.seek 25ml @17:53:01.09 PSR.select :air @17:53:01.21 PS.seek 2ml @17:53:17.30 PTV.dial :puck @17:53:18.00 PSV.dial PTV @17:53:18.72 PBV.dial :waste1 @17:53:19.12 PS.seek 1.5ml @17:53:22.12 Stroke completed -- waiting for pressure to equalize ... @17:53:22.13 SS.reconfigure :exhale @17:53:22.17 SS.hold @17:53:23.65 Intake.select :close @17:53:23.80 SSV.dial :exhaust @17:53:24.54 Exhaust.select :open @17:53:24.65 SS.seek 0ml @17:53:24.95 delay 3 seconds @17:53:27.97 PBV.dial between PSV and waste1 @17:53:28.34 PS.seek 0ml @17:53:33.82 Exhaust.select :close @17:53:33.93 SSV.dial CBV @17:53:33.97 CBV.dial SSV @17:53:34.70 Allowing 130s for 25.0ml of remaining 775.0ml @17:53:34.71 Intake.select :open @17:53:36.87 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:53:36.88 SS.reconfigure :inhaling 1.27ml/s @17:53:36.93 SS.seek 25ml @17:53:44.33 delay 2:00 @17:53:58.41 Stroke completed -- waiting for pressure to equalize ... @17:53:58.42 SS.reconfigure :exhale @17:53:58.47 SS.hold @17:54:00.04 Intake.select :close @17:54:00.18 SSV.dial :exhaust @17:54:00.93 Exhaust.select :open @17:54:01.04 SS.seek 0ml @17:54:10.33 Exhaust.select :close @17:54:10.44 SSV.dial CBV @17:54:10.47 CBV.dial SSV @17:54:11.23 Allowing 130s for 25.0ml of remaining 750.0ml @17:54:11.24 Intake.select :open @17:54:13.39 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:54:13.41 SS.reconfigure :inhaling 1.27ml/s @17:54:13.46 SS.seek 25ml @17:54:34.82 Stroke completed -- waiting for pressure to equalize ... @17:54:34.83 SS.reconfigure :exhale @17:54:34.87 SS.hold @17:54:36.82 Intake.select :close @17:54:36.96 SSV.dial :exhaust @17:54:37.69 Exhaust.select :open @17:54:37.81 SS.seek 0ml @17:54:46.74 Exhaust.select :close @17:54:46.85 SSV.dial CBV @17:54:46.88 CBV.dial SSV @17:54:47.60 Allowing 130s for 25.0ml of remaining 725.0ml @17:54:47.61 Intake.select :open @17:54:49.77 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:54:49.78 SS.reconfigure :inhaling 1.27ml/s @17:54:49.83 SS.seek 25ml @17:55:04.45 Vacuum too high -- waiting up to 40 seconds @17:55:04.46 SS.reconfigure :exhale @17:55:04.50 SS.hold @17:55:04.91 Intake @11.1psia, vacuum from 5.6 to 8.6psi @17:55:04.93 SS.reconfigure :inhaling 1.14ml/s @17:55:04.97 SS.seek 25ml @17:55:13.99 Stroke completed -- waiting for pressure to equalize ... @17:55:14.00 SS.reconfigure :exhale @17:55:14.04 SS.hold @17:55:15.69 Intake.select :close @17:55:15.85 SSV.dial :exhaust @17:55:16.57 Exhaust.select :open @17:55:16.68 SS.seek 0ml @17:55:25.52 Exhaust.select :close @17:55:25.63 SSV.dial CBV @17:55:25.66 CBV.dial SSV @17:55:26.38 Allowing 130s for 25.0ml of remaining 700.0ml @17:55:26.39 Intake.select :open @17:55:28.55 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:55:28.56 SS.reconfigure :inhaling 1.14ml/s @17:55:28.61 SS.seek 25ml @17:55:44.35 recoverPSRreagent08 @17:55:44.36 PTV.dial :puck @17:55:44.37 PBV.dial :waste1 @17:55:44.72 delay 30 seconds @17:55:53.08 Stroke completed -- waiting for pressure to equalize ... @17:55:53.09 SS.reconfigure :exhale @17:55:53.13 SS.hold @17:55:54.77 Intake.select :close @17:55:54.92 SSV.dial :exhaust @17:55:55.64 Exhaust.select :open @17:55:56.23 SS.seek 0ml @17:56:05.16 Exhaust.select :close @17:56:05.27 SSV.dial CBV @17:56:05.30 CBV.dial SSV @17:56:06.04 Allowing 130s for 25.0ml of remaining 675.0ml @17:56:06.05 Intake.select :open @17:56:08.21 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:56:08.23 SS.reconfigure :inhaling 1.14ml/s @17:56:08.27 SS.seek 25ml @17:56:14.73 PBV.dial between PSV and waste1 @17:56:15.09 PSR.select :air @17:56:15.11 PSV.dial PSR @17:56:15.83 PS.reconfigure :fast @17:56:15.87 PS.seek 2.5ml @17:56:32.78 Stroke completed -- waiting for pressure to equalize ... @17:56:32.79 SS.reconfigure :exhale @17:56:32.84 SS.hold @17:56:34.82 Intake.select :close @17:56:34.97 SSV.dial :exhaust @17:56:35.69 Exhaust.select :open @17:56:35.80 SS.seek 0ml @17:56:38.42 PSV.dial PTV @17:56:39.14 delay 5 seconds @17:56:44.15 PSV.dial PSR @17:56:44.81 Exhaust.select :close @17:56:44.89 delay 5 seconds @17:56:44.92 SSV.dial CBV @17:56:44.95 CBV.dial SSV @17:56:45.69 Allowing 130s for 25.0ml of remaining 650.0ml @17:56:45.70 Intake.select :open @17:56:47.86 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:56:47.88 SS.reconfigure :inhaling 1.14ml/s @17:56:47.92 SS.seek 25ml @17:56:49.90 PSV.dial PTV @17:56:50.62 delay 5 seconds @17:56:55.64 PSV.dial :air @17:56:56.36 PS.seek 0ml @17:57:12.44 Stroke completed -- waiting for pressure to equalize ... @17:57:12.45 SS.reconfigure :exhale @17:57:12.49 SS.hold @17:57:14.89 Intake.select :close @17:57:15.03 SSV.dial :exhaust @17:57:15.77 Exhaust.select :open @17:57:15.88 SS.seek 0ml @17:57:18.70 washCycleDA #2 of 2 @17:57:18.71 PSR.select :air @17:57:18.72 PSV.dial PSR @17:57:20.03 Pulling 0.5ml of PSR.wash @17:57:20.04 PSR.select :wash @17:57:20.15 PS.reconfigure :default @17:57:20.20 PS.seek 0.5ml @17:57:25.23 Exhaust.select :close @17:57:25.34 SSV.dial CBV @17:57:25.37 CBV.dial SSV @17:57:26.11 delay 5 seconds @17:57:26.14 Allowing 130s for 25.0ml of remaining 625.0ml @17:57:26.16 Intake.select :open @17:57:28.31 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:57:28.32 SS.reconfigure :inhaling 1.14ml/s @17:57:28.37 SS.seek 25ml @17:57:31.13 PSR.select :air @17:57:31.24 PS.seek 2ml @17:57:44.26 Vacuum too high -- waiting up to 40 seconds @17:57:44.27 SS.reconfigure :exhale @17:57:44.31 SS.hold @17:57:44.71 Intake @11.7psia, vacuum from 6.2 to 9.2psi @17:57:44.73 SS.reconfigure :inhaling 1.03ml/s @17:57:44.77 SS.seek 25ml @17:57:47.29 PTV.dial :puck @17:57:47.30 PSV.dial PTV @17:57:48.01 PBV.dial :waste1 @17:57:48.43 PS.seek 1.5ml @17:57:53.72 Stroke completed -- waiting for pressure to equalize ... @17:57:53.74 SS.reconfigure :exhale @17:57:53.78 SS.hold @17:57:54.31 delay 3 seconds @17:57:55.72 Intake.select :close @17:57:55.87 SSV.dial :exhaust @17:57:56.59 Exhaust.select :open @17:57:56.71 SS.seek 0ml @17:57:57.33 PBV.dial between PSV and waste1 @17:57:57.86 PS.seek 0ml @17:58:05.58 Exhaust.select :close @17:58:05.69 SSV.dial CBV @17:58:05.72 CBV.dial SSV @17:58:06.47 Allowing 130s for 25.0ml of remaining 600.0ml @17:58:06.48 Intake.select :open @17:58:08.64 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:58:08.66 SS.reconfigure :inhaling 1.03ml/s @17:58:08.70 SS.seek 25ml @17:58:13.85 delay 2:00 @17:58:35.64 Stroke completed -- waiting for pressure to equalize ... @17:58:35.65 SS.reconfigure :exhale @17:58:35.69 SS.hold @17:58:38.60 Intake.select :close @17:58:38.75 SSV.dial :exhaust @17:58:39.50 Exhaust.select :open @17:58:39.61 SS.seek 0ml @17:58:48.95 Exhaust.select :close @17:58:49.06 SSV.dial CBV @17:58:49.08 CBV.dial SSV @17:58:49.84 Allowing 130s for 25.0ml of remaining 575.0ml @17:58:49.86 Intake.select :open @17:58:52.02 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:58:52.04 SS.reconfigure :inhaling 1.03ml/s @17:58:52.08 SS.seek 25ml @17:59:13.79 Vacuum too high -- waiting up to 40 seconds @17:59:13.80 SS.reconfigure :exhale @17:59:13.84 SS.hold @17:59:14.24 Intake @12.1psia, vacuum from 6.6 to 9.6psi @17:59:14.26 SS.reconfigure :inhaling 0.93ml/s @17:59:14.31 SS.seek 25ml @17:59:19.49 Stroke completed -- waiting for pressure to equalize ... @17:59:19.50 SS.reconfigure :exhale @17:59:19.54 SS.hold @17:59:21.49 Intake.select :close @17:59:21.63 SSV.dial :exhaust @17:59:22.36 Exhaust.select :open @17:59:22.47 SS.seek 0ml @17:59:31.93 Exhaust.select :close @17:59:32.04 SSV.dial CBV @17:59:32.07 CBV.dial SSV @17:59:32.81 Allowing 130s for 25.0ml of remaining 550.0ml @17:59:32.82 Intake.select :open @17:59:34.98 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:59:34.99 SS.reconfigure :inhaling 0.93ml/s @17:59:35.04 SS.seek 25ml @18:00:03.77 Stroke completed -- waiting for pressure to equalize ... @18:00:03.79 SS.reconfigure :exhale @18:00:03.83 SS.hold @18:00:06.73 Intake.select :close @18:00:06.88 SSV.dial :exhaust @18:00:07.60 Exhaust.select :open @18:00:07.72 SS.seek 0ml @18:00:13.87 recoverPSRreagent08 @18:00:13.88 PTV.dial :puck @18:00:13.89 PBV.dial :waste1 @18:00:14.24 delay 30 seconds @18:00:17.06 Exhaust.select :close @18:00:17.17 SSV.dial CBV @18:00:17.19 CBV.dial SSV @18:00:17.95 Allowing 130s for 25.0ml of remaining 525.0ml @18:00:17.96 Intake.select :open @18:00:20.12 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:00:20.13 SS.reconfigure :inhaling 0.93ml/s @18:00:20.18 SS.seek 25ml @18:00:44.25 PBV.dial between PSV and waste1 @18:00:44.61 PSR.select :air @18:00:44.63 PSV.dial PSR @18:00:45.35 PS.reconfigure :fast @18:00:45.40 PS.seek 2.5ml @18:00:46.47 Vacuum too high -- waiting up to 40 seconds @18:00:46.48 SS.reconfigure :exhale @18:00:46.52 SS.hold @18:00:46.92 Intake @12.3psia, vacuum from 6.8 to 9.8psi @18:00:46.94 SS.reconfigure :inhaling 0.84ml/s @18:00:46.98 SS.seek 25ml @18:00:49.96 Stroke completed -- waiting for pressure to equalize ... @18:00:49.97 SS.reconfigure :exhale @18:00:50.01 SS.hold @18:00:52.37 Intake.select :close @18:00:52.52 SSV.dial :exhaust @18:00:53.24 Exhaust.select :open @18:00:53.36 SS.seek 0ml @18:01:02.63 Exhaust.select :close @18:01:02.74 SSV.dial CBV @18:01:02.77 CBV.dial SSV @18:01:03.51 Allowing 130s for 25.0ml of remaining 500.0ml @18:01:03.52 Intake.select :open @18:01:05.69 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:01:05.70 SS.reconfigure :inhaling 0.84ml/s @18:01:05.75 SS.seek 25ml @18:01:08.16 PSV.dial PTV @18:01:08.88 delay 5 seconds @18:01:13.90 PSV.dial PSR @18:01:14.61 delay 5 seconds @18:01:19.63 PSV.dial PTV @18:01:20.35 delay 5 seconds @18:01:25.37 PSV.dial :air @18:01:26.09 PS.seek 0ml @18:01:37.13 Stroke completed -- waiting for pressure to equalize ... @18:01:37.14 SS.reconfigure :exhale @18:01:37.19 SS.hold @18:01:40.08 Intake.select :close @18:01:40.23 SSV.dial :exhaust @18:01:40.96 Exhaust.select :open @18:01:41.07 SS.seek 0ml @18:01:48.56 getPMLReagent08 @18:01:48.57 PRV.dial :air @18:01:49.02 delay 5 seconds @18:01:50.10 Exhaust.select :close @18:01:50.21 SSV.dial CBV @18:01:50.24 CBV.dial SSV @18:01:50.99 Allowing 130s for 25.0ml of remaining 475.0ml @18:01:51.01 Intake.select :open @18:01:53.17 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:01:53.18 SS.reconfigure :inhaling 0.84ml/s @18:01:53.23 SS.seek 25ml @18:01:54.03 PTV.dial :PRVmixing @18:01:55.27 delay 5 seconds @18:02:00.29 PRV.dial :ab2 @18:02:00.72 PTV.dial :PRVmixing @18:02:00.73 PSV.dial :mixloop @18:02:01.18 PS.reconfigure :default @18:02:01.22 PS.seek 0.3ml @18:02:05.09 delay 2 seconds @18:02:07.11 PRV.dial :air @18:02:07.61 PS.seek 0.55ml @18:02:10.91 addPMLReagent08 @18:02:10.92 PSV.dial :air @18:02:11.37 PS.seek 2ml @18:02:22.00 Vacuum too high -- waiting up to 40 seconds @18:02:22.02 SS.reconfigure :exhale @18:02:22.06 SS.hold @18:02:23.35 Intake @13.3psia, vacuum from 7.8 to 10.8psi @18:02:23.36 SS.reconfigure :inhaling 0.76ml/s @18:02:23.41 SS.seek 25ml @18:02:26.97 PSV.dial :PML @18:02:27.42 PTV.dial :mixing @18:02:27.58 Stroke completed -- waiting for pressure to equalize ... @18:02:27.59 SS.reconfigure :exhale @18:02:27.64 SS.hold @18:02:28.17 PBV.dial :waste1 @18:02:28.57 PS.seek 1.85ml @18:02:29.58 Intake.select :close @18:02:29.73 SSV.dial :exhaust @18:02:30.46 Exhaust.select :open @18:02:30.57 SS.seek 0ml @18:02:31.00 PBV.dial between PSV and waste1 @18:02:31.37 PS.seek 0ml @18:02:40.00 Exhaust.select :close @18:02:40.11 SSV.dial CBV @18:02:40.14 CBV.dial SSV @18:02:40.88 Allowing 130s for 25.0ml of remaining 450.0ml @18:02:40.90 Intake.select :open @18:02:43.05 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:02:43.54 SS.reconfigure :inhaling 0.76ml/s @18:02:43.60 SS.seek 25ml @18:02:50.97 delay 2 seconds @18:02:52.99 delay 8:00 @18:03:18.21 Stroke completed -- waiting for pressure to equalize ... @18:03:18.22 SS.reconfigure :exhale @18:03:18.26 SS.hold @18:03:21.73 Intake.select :close @18:03:21.87 SSV.dial :exhaust @18:03:22.60 Exhaust.select :open @18:03:22.71 SS.seek 0ml @18:03:32.08 Exhaust.select :close @18:03:32.19 SSV.dial CBV @18:03:32.23 CBV.dial SSV @18:03:32.96 Allowing 130s for 25.0ml of remaining 425.0ml @18:03:32.97 Intake.select :open @18:03:35.13 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:03:35.14 SS.reconfigure :inhaling 0.76ml/s @18:03:35.19 SS.seek 25ml @18:04:09.85 Stroke completed -- waiting for pressure to equalize ... @18:04:09.86 SS.reconfigure :exhale @18:04:09.90 SS.hold @18:04:13.98 Intake.select :close @18:04:14.12 SSV.dial :exhaust @18:04:14.85 Exhaust.select :open @18:04:14.96 SS.seek 0ml @18:04:23.97 Exhaust.select :close @18:04:24.08 SSV.dial CBV @18:04:24.12 CBV.dial SSV @18:04:24.83 Allowing 130s for 25.0ml of remaining 400.0ml @18:04:24.84 Intake.select :open @18:04:27.00 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:04:27.02 SS.reconfigure :inhaling 0.76ml/s @18:04:27.06 SS.seek 25ml @18:04:55.18 Vacuum too high -- waiting up to 40 seconds @18:04:55.19 SS.reconfigure :exhale @18:04:55.23 SS.hold @18:04:56.52 Intake @13.4psia, vacuum from 7.9 to 10.9psi @18:04:56.54 SS.reconfigure :inhaling 0.69ml/s @18:04:56.59 SS.seek 25ml @18:05:05.66 Stroke completed -- waiting for pressure to equalize ... @18:05:05.67 SS.reconfigure :exhale @18:05:05.71 SS.hold @18:05:09.16 Intake.select :close @18:05:09.30 SSV.dial :exhaust @18:05:10.03 Exhaust.select :open @18:05:10.14 SS.seek 0ml @18:05:19.19 Exhaust.select :close @18:05:19.29 SSV.dial CBV @18:05:19.32 CBV.dial SSV @18:05:20.08 Allowing 130s for 25.0ml of remaining 375.0ml @18:05:20.09 Intake.select :open @18:05:22.25 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:05:22.26 SS.reconfigure :inhaling 0.69ml/s @18:05:22.31 SS.seek 25ml @18:06:00.61 Stroke completed -- waiting for pressure to equalize ... @18:06:00.62 SS.reconfigure :exhale @18:06:00.66 SS.hold @18:06:05.52 Intake.select :close @18:06:05.66 SSV.dial :exhaust @18:06:06.89 Exhaust.select :open @18:06:07.00 SS.seek 0ml @18:06:16.45 Exhaust.select :close @18:06:16.56 SSV.dial CBV @18:06:16.59 CBV.dial SSV @18:06:17.31 Allowing 130s for 25.0ml of remaining 350.0ml @18:06:17.33 Intake.select :open @18:06:19.49 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:06:19.50 SS.reconfigure :inhaling 0.69ml/s @18:06:19.55 SS.seek 25ml @18:06:45.77 Vacuum too high -- waiting up to 40 seconds @18:06:45.78 SS.reconfigure :exhale @18:06:45.83 SS.hold @18:06:47.48 Intake @14.7psia, vacuum from 9.2 to 12.2psi @18:06:47.49 SS.reconfigure :inhaling 0.61ml/s @18:06:47.54 SS.seek 25ml @18:07:01.31 Stroke completed -- waiting for pressure to equalize ... @18:07:01.32 SS.reconfigure :exhale @18:07:01.36 SS.hold @18:07:05.43 Intake.select :close @18:07:05.57 SSV.dial :exhaust @18:07:06.35 Exhaust.select :open @18:07:06.47 SS.seek 0ml @18:07:15.50 Exhaust.select :close @18:07:15.60 SSV.dial CBV @18:07:15.63 CBV.dial SSV @18:07:16.39 Allowing 130s for 25.0ml of remaining 325.0ml @18:07:16.40 Intake.select :open @18:07:18.56 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:07:18.57 SS.reconfigure :inhaling 0.61ml/s @18:07:18.62 SS.seek 25ml @18:08:02.07 Stroke completed -- waiting for pressure to equalize ... @18:08:02.08 SS.reconfigure :exhale @18:08:02.13 SS.hold @18:08:07.76 Intake.select :close @18:08:07.90 SSV.dial :exhaust @18:08:08.64 Exhaust.select :open @18:08:08.75 SS.seek 0ml @18:08:18.09 Exhaust.select :close @18:08:18.19 SSV.dial CBV @18:08:18.22 CBV.dial SSV @18:08:18.95 Allowing 130s for 25.0ml of remaining 300.0ml @18:08:18.96 Intake.select :open @18:08:21.11 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:08:21.13 SS.reconfigure :inhaling 0.61ml/s @18:08:21.18 SS.seek 25ml @18:08:49.08 Vacuum too high -- waiting up to 40 seconds @18:08:49.09 SS.reconfigure :exhale @18:08:49.13 SS.hold @18:08:51.52 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:08:51.54 SS.reconfigure :inhaling 0.56ml/s @18:08:51.59 SS.seek 25ml @18:09:08.15 Stroke completed -- waiting for pressure to equalize ... @18:09:08.16 SS.reconfigure :exhale @18:09:08.20 SS.hold @18:09:12.96 Intake.select :close @18:09:13.10 SSV.dial :exhaust @18:09:13.84 Exhaust.select :open @18:09:14.44 SS.seek 0ml @18:09:23.44 Exhaust.select :close @18:09:23.55 SSV.dial CBV @18:09:23.58 CBV.dial SSV @18:09:24.34 Allowing 130s for 25.0ml of remaining 275.0ml @18:09:24.35 Intake.select :open @18:09:26.51 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:09:26.52 SS.reconfigure :inhaling 0.56ml/s @18:09:26.57 SS.seek 25ml @18:10:14.51 Stroke completed -- waiting for pressure to equalize ... @18:10:14.52 SS.reconfigure :exhale @18:10:14.56 SS.hold @18:10:20.97 Intake.select :close @18:10:21.11 SSV.dial :exhaust @18:10:21.85 Exhaust.select :open @18:10:21.96 SS.seek 0ml @18:10:31.66 Exhaust.select :close @18:10:31.77 SSV.dial CBV @18:10:31.80 CBV.dial SSV @18:10:32.52 Allowing 130s for 25.0ml of remaining 250.0ml @18:10:32.54 Intake.select :open @18:10:34.70 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:10:34.71 SS.reconfigure :inhaling 0.56ml/s @18:10:34.76 SS.seek 25ml @18:10:53.01 recoverPRVreagent08 @18:10:53.02 PTV.dial :mixing @18:10:53.03 PBV.dial :waste1 @18:10:53.38 delay 30 seconds @18:11:03.95 Vacuum too high -- waiting up to 40 seconds @18:11:03.96 SS.reconfigure :exhale @18:11:04.01 SS.hold @18:11:06.05 Intake @14.9psia, vacuum from 9.4 to 12.4psi @18:11:06.07 SS.reconfigure :inhaling 0.50ml/s @18:11:06.12 SS.seek 25ml @18:11:23.40 PBV.dial between PSV and waste1 @18:11:23.75 PSV.dial :air @18:11:24.21 PS.reconfigure :fast @18:11:24.25 PS.seek 2.5ml @18:11:27.13 Stroke completed -- waiting for pressure to equalize ... @18:11:27.14 SS.reconfigure :exhale @18:11:27.18 SS.hold @18:11:32.72 Intake.select :close @18:11:32.87 SSV.dial :exhaust @18:11:33.60 Exhaust.select :open @18:11:33.71 SS.seek 0ml @18:11:42.71 Exhaust.select :close @18:11:42.81 SSV.dial CBV @18:11:42.84 CBV.dial SSV @18:11:43.60 Allowing 130s for 25.0ml of remaining 225.0ml @18:11:43.61 Intake.select :open @18:11:45.77 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:11:45.78 SS.reconfigure :inhaling 0.50ml/s @18:11:45.83 SS.seek 25ml @18:11:46.86 PSV.dial :mixloop @18:11:47.31 delay 2 seconds @18:11:49.33 PSV.dial :air @18:11:49.78 delay 2 seconds @18:11:51.80 PSV.dial :mixloop @18:11:52.25 delay 2 seconds @18:11:54.27 PSV.dial :air @18:11:54.73 PS.seek 0ml @18:12:17.27 washCycleDA #1 of 3 @18:12:17.28 PSR.select :air @18:12:17.29 PSV.dial PSR @18:12:18.60 Pulling 0.5ml of PSR.wash @18:12:18.61 PSR.select :wash @18:12:18.72 PS.reconfigure :default @18:12:18.76 PS.seek 0.5ml @18:12:24.71 delay 5 seconds @18:12:29.72 PSR.select :air @18:12:29.83 PS.seek 2ml @18:12:40.08 Stroke completed -- waiting for pressure to equalize ... @18:12:40.09 SS.reconfigure :exhale @18:12:40.13 SS.hold @18:12:45.92 PTV.dial :puck @18:12:46.62 PSV.dial PTV @18:12:47.13 Intake.select :close @18:12:47.27 SSV.dial :exhaust @18:12:47.34 PBV.dial :waste1 @18:12:47.76 PS.seek 1.5ml @18:12:48.00 Exhaust.select :open @18:12:48.11 SS.seek 0ml @18:12:53.64 delay 3 seconds @18:12:56.66 PBV.dial between PSV and waste1 @18:12:57.02 PS.seek 0ml @18:12:57.11 Exhaust.select :close @18:12:57.22 SSV.dial CBV @18:12:57.24 CBV.dial SSV @18:12:57.97 Allowing 130s for 25.0ml of remaining 200.0ml @18:12:57.98 Intake.select :open @18:13:00.14 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:13:00.16 SS.reconfigure :inhaling 0.50ml/s @18:13:00.20 SS.seek 25ml @18:13:13.08 delay 2:00 @18:13:34.98 Vacuum too high -- waiting up to 40 seconds @18:13:34.99 SS.reconfigure :exhale @18:13:35.03 SS.hold @18:13:38.03 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:13:38.05 SS.reconfigure :inhaling 0.46ml/s @18:13:38.09 SS.seek 25ml @18:13:59.65 Stroke completed -- waiting for pressure to equalize ... @18:13:59.67 SS.reconfigure :exhale @18:13:59.71 SS.hold @18:14:06.19 Intake.select :close @18:14:06.33 SSV.dial :exhaust @18:14:07.06 Exhaust.select :open @18:14:07.17 SS.seek 0ml @18:14:16.22 Exhaust.select :close @18:14:16.32 SSV.dial CBV @18:14:16.35 CBV.dial SSV @18:14:17.11 Allowing 130s for 25.0ml of remaining 175.0ml @18:14:17.12 Intake.select :open @18:14:19.28 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:14:19.29 SS.reconfigure :inhaling 0.46ml/s @18:14:19.34 SS.seek 25ml @18:15:13.10 recoverPSRreagent08 @18:15:13.11 PTV.dial :puck @18:15:13.12 PBV.dial :waste1 @18:15:13.46 delay 30 seconds @18:15:18.85 Stroke completed -- waiting for pressure to equalize ... @18:15:18.87 SS.reconfigure :exhale @18:15:18.91 SS.hold @18:15:26.65 Intake.select :close @18:15:26.79 SSV.dial :exhaust @18:15:27.51 Exhaust.select :open @18:15:27.62 SS.seek 0ml @18:15:37.07 Exhaust.select :close @18:15:37.18 SSV.dial CBV @18:15:37.22 CBV.dial SSV @18:15:38.35 Allowing 130s for 25.0ml of remaining 150.0ml @18:15:38.45 Intake.select :open @18:15:40.61 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:15:40.62 SS.reconfigure :inhaling 0.46ml/s @18:15:40.67 SS.seek 25ml @18:15:43.48 PBV.dial between PSV and waste1 @18:15:43.84 PSR.select :air @18:15:43.86 PSV.dial PSR @18:15:44.58 PS.reconfigure :fast @18:15:44.62 PS.seek 2.5ml @18:16:07.26 PSV.dial PTV @18:16:07.98 delay 5 seconds @18:16:13.00 PSV.dial PSR @18:16:13.71 delay 5 seconds @18:16:16.44 Vacuum too high -- waiting up to 40 seconds @18:16:16.45 SS.reconfigure :exhale @18:16:16.50 SS.hold @18:16:18.73 PSV.dial PTV @18:16:19.46 delay 5 seconds @18:16:19.54 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:16:19.56 SS.reconfigure :inhaling 0.41ml/s @18:16:19.61 SS.seek 25ml @18:16:24.49 PSV.dial :air @18:16:25.22 PS.seek 0ml @18:16:45.96 Stroke completed -- waiting for pressure to equalize ... @18:16:45.97 SS.reconfigure :exhale @18:16:46.02 SS.hold @18:16:47.82 washCycleDA #2 of 3 @18:16:47.83 PSR.select :air @18:16:47.85 PSV.dial PSR @18:16:49.26 Pulling 0.5ml of PSR.wash @18:16:49.27 PSR.select :wash @18:16:49.38 PS.reconfigure :default @18:16:49.44 PS.seek 0.5ml @18:16:52.56 Intake.select :close @18:16:52.71 SSV.dial :exhaust @18:16:53.43 Exhaust.select :open @18:16:53.54 SS.seek 0ml @18:16:55.35 delay 5 seconds @18:17:00.37 PSR.select :air @18:17:00.48 PS.seek 2ml @18:17:02.59 Exhaust.select :close @18:17:02.69 SSV.dial CBV @18:17:02.72 CBV.dial SSV @18:17:03.48 Allowing 130s for 25.0ml of remaining 125.0ml @18:17:03.49 Intake.select :open @18:17:05.65 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:17:05.67 SS.reconfigure :inhaling 0.41ml/s @18:17:05.72 SS.seek 25ml @18:17:16.57 PTV.dial :puck @18:17:16.58 PSV.dial PTV @18:17:17.30 PBV.dial :waste1 @18:17:17.72 PS.seek 1.5ml @18:17:23.69 delay 3 seconds @18:17:26.71 PBV.dial between PSV and waste1 @18:17:27.08 PS.seek 0ml @18:17:43.19 delay 2:00 @18:18:14.68 Stroke completed -- waiting for pressure to equalize ... @18:18:14.69 SS.reconfigure :exhale @18:18:14.73 SS.hold @18:18:23.18 Intake.select :close @18:18:23.32 SSV.dial :exhaust @18:18:24.05 Exhaust.select :open @18:18:24.16 SS.seek 0ml @18:18:33.19 Exhaust.select :close @18:18:33.30 SSV.dial CBV @18:18:33.34 CBV.dial SSV @18:18:34.07 Allowing 130s for 25.0ml of remaining 100.0ml @18:18:34.08 Intake.select :open @18:18:36.65 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:18:36.75 SS.reconfigure :inhaling 0.41ml/s @18:18:36.79 SS.seek 25ml @18:19:43.21 recoverPSRreagent08 @18:19:43.22 PTV.dial :puck @18:19:43.24 PBV.dial :waste1 @18:19:43.37 Vacuum too high -- waiting up to 40 seconds @18:19:43.38 SS.reconfigure :exhale @18:19:43.43 SS.hold @18:19:43.58 delay 30 seconds @18:19:47.60 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:19:47.62 SS.reconfigure :inhaling 0.37ml/s @18:19:47.67 SS.seek 25ml @18:19:51.56 Stroke completed -- waiting for pressure to equalize ... @18:19:51.58 SS.reconfigure :exhale @18:19:51.62 SS.hold @18:19:56.39 Intake.select :close @18:19:56.53 SSV.dial :exhaust @18:19:57.27 Exhaust.select :open @18:19:57.38 SS.seek 0ml @18:20:06.83 Exhaust.select :close @18:20:06.94 SSV.dial CBV @18:20:06.96 CBV.dial SSV @18:20:07.69 Allowing 130s for 25.0ml of remaining 75.0ml @18:20:07.70 Intake.select :open @18:20:09.86 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:20:09.88 SS.reconfigure :inhaling 0.37ml/s @18:20:09.92 SS.seek 25ml @18:20:13.60 PBV.dial between PSV and waste1 @18:20:13.96 PSR.select :air @18:20:13.97 PSV.dial PSR @18:20:14.69 PS.reconfigure :fast @18:20:14.74 PS.seek 2.5ml @18:20:37.44 PSV.dial PTV @18:20:38.16 delay 5 seconds @18:20:43.18 PSV.dial PSR @18:20:43.89 delay 5 seconds @18:20:48.91 PSV.dial PTV @18:20:49.63 delay 5 seconds @18:20:54.65 PSV.dial :air @18:20:55.37 PS.seek 0ml @18:21:17.97 washCycleDA #3 of 3 @18:21:17.98 PSR.select :air @18:21:18.00 PSV.dial PSR @18:21:19.41 Pulling 0.5ml of PSR.wash @18:21:19.42 PSR.select :wash @18:21:19.54 PS.reconfigure :default @18:21:19.58 PS.seek 0.5ml @18:21:25.55 delay 5 seconds @18:21:28.95 Stroke completed -- waiting for pressure to equalize ... @18:21:28.96 SS.reconfigure :exhale @18:21:29.01 SS.hold @18:21:30.61 PSR.select :air @18:21:30.72 PS.seek 2ml @18:21:36.47 Intake.select :close @18:21:36.61 SSV.dial :exhaust @18:21:37.34 Exhaust.select :open @18:21:37.45 SS.seek 0ml @18:21:46.72 PTV.dial :puck @18:21:46.73 PSV.dial PTV @18:21:46.79 Exhaust.select :close @18:21:46.89 SSV.dial CBV @18:21:46.92 CBV.dial SSV @18:21:47.45 PBV.dial :waste1 @18:21:47.67 Allowing 130s for 25.0ml of remaining 50.0ml @18:21:47.69 Intake.select :open @18:21:47.86 PS.seek 1.5ml @18:21:49.88 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:21:49.90 SS.reconfigure :inhaling 0.37ml/s @18:21:49.94 SS.seek 25ml @18:21:53.81 delay 3 seconds @18:21:56.83 PBV.dial between PSV and waste1 @18:21:57.69 PS.seek 0ml @18:22:13.78 delay 2:00 @18:23:08.71 Stroke completed -- waiting for pressure to equalize ... @18:23:08.73 SS.reconfigure :exhale @18:23:08.77 SS.hold @18:23:18.44 Intake.select :close @18:23:18.58 SSV.dial :exhaust @18:23:19.31 Exhaust.select :open @18:23:19.43 SS.seek 0ml @18:23:28.79 Exhaust.select :close @18:23:28.90 SSV.dial CBV @18:23:28.94 CBV.dial SSV @18:23:29.67 Allowing 130s for 25.0ml of remaining 25.0ml @18:23:29.68 Intake.select :open @18:23:31.84 Intake @16.0psia, vacuum from 10.0 to 13.0psi @18:23:31.86 SS.reconfigure :inhaling 0.37ml/s @18:23:31.90 SS.seek 24.96ml @18:24:13.80 recoverPSRreagent08 @18:24:13.82 PTV.dial :puck @18:24:13.83 PBV.dial :waste1 @18:24:14.19 delay 30 seconds @18:24:33.71 Vacuum too high -- waiting up to 40 seconds @18:24:33.72 SS.reconfigure :exhale @18:24:33.76 SS.hold @18:24:37.94 SS.reconfigure :inhaling 0.33ml/s @18:24:37.99 SS.seek 24.96ml @18:24:44.20 PBV.dial between PSV and waste1 @18:24:44.56 PSR.select :air @18:24:44.58 PSV.dial PSR @18:24:45.30 PS.reconfigure :fast @18:24:45.34 PS.seek 2.5ml @18:24:58.74 Stroke completed -- waiting for pressure to equalize ... @18:24:58.75 SS.reconfigure :exhale @18:24:58.80 SS.hold @18:25:06.16 Intake.select :close @18:25:06.30 SSV.dial :exhaust @18:25:07.88 Exhaust.select :open @18:25:07.96 PSV.dial PTV @18:25:08.02 SS.seek 0ml @18:25:08.68 delay 5 seconds @18:25:13.70 PSV.dial PSR @18:25:14.42 delay 5 seconds @18:25:17.03 Exhaust.select :close @18:25:17.13 Sampled 1000.0ml @18:25:17.15 Drying puck before fixing for 20:00 @18:25:17.16 CBV.dial :waste2b @18:25:17.87 delay 1:00 @18:25:19.43 PSV.dial PTV @18:25:20.15 delay 5 seconds @18:25:25.17 PSV.dial :air @18:25:25.89 PS.seek 0ml @18:25:48.30 Gate.power :camera,:ON @18:25:48.32 Mixing DA Substrates @18:25:48.33 PSR.select :air @18:25:48.34 PSV.dial PSR @18:25:50.00 Pulling 0.25ml of PSR.sub1 @18:25:50.05 PSR.select :sub1 @18:25:50.31 PS.reconfigure :default @18:25:50.49 PS.seek 0.25ml @18:25:53.93 delay 5 seconds @18:25:59.00 Pulling 0.25ml of PSR.sub2 @18:25:59.01 PSR.select :sub2 @18:25:59.12 PS.seek 0.5ml @18:26:02.49 delay 5 seconds @18:26:07.51 PSR.select :air @18:26:07.62 PS.seek 2ml @18:26:17.89 CSV.dial :air @18:26:18.84 CS.reconfigure :fast @18:26:18.90 CS.seek 10ml @18:26:23.68 PTV.dial :puck @18:26:23.70 PSV.dial PTV @18:26:24.43 PBV.dial :waste1 @18:26:24.85 PS.seek 1.5ml @18:26:30.77 delay 3 seconds @18:26:33.79 PBV.dial between PSV and waste1 @18:26:34.16 PS.seek 0ml @18:26:41.54 Pumping wcr puck from tube 3 up to 35psi... @18:26:41.55 CBV.dial SSV @18:26:42.84 CBV.dial between CSV and waste2b @18:26:43.77 CSV.dial between air and CTV @18:26:44.09 CS.seek 7.19ml @18:26:50.27 delay 10 seconds @18:26:51.10 CTV.dial CSV,avoiding: :air @18:26:51.13 CSV.dial CTV @18:26:51.92 CS.reconfigure :pressurize @18:26:51.97 CS.seek 0ml @18:27:00.29 recoverPSRreagent08 @18:27:00.30 PTV.dial :puck @18:27:00.32 PBV.dial :waste1 @18:27:00.66 delay 30 seconds @18:27:05.56 CBV.dial :waste2b @18:27:05.88 CS.reconfigure :default @18:27:05.93 CS.seek 0ml @18:27:11.63 delay 1:00 @18:27:30.69 PBV.dial between PSV and waste1 @18:27:31.04 PSR.select :air @18:27:31.05 PSV.dial PSR @18:27:31.78 PS.reconfigure :fast @18:27:31.82 PS.seek 2.5ml @18:27:54.42 PSV.dial PTV @18:27:55.15 delay 5 seconds @18:28:00.16 PSV.dial PSR @18:28:00.88 delay 5 seconds @18:28:05.90 PSV.dial PTV @18:28:06.61 delay 5 seconds @18:28:11.63 PSV.dial :air @18:28:11.68 CTV.dial :air,avoiding: :intake @18:28:12.35 PS.seek 0ml @18:28:12.96 Fix #1 of 2 (with RNAlater) for 10:00 @18:28:12.98 CSV.dial CSR @18:28:13.46 Pulling 2ml of CSR.RNAlater @18:28:13.47 CSR.select :RNAlater @18:28:13.59 CS.seek 2ml @18:28:19.41 delay 5 seconds @18:28:24.43 CSR.select :air @18:28:24.54 CS.reconfigure :fast @18:28:24.59 CS.seek 10ml @18:28:34.93 Additional air clear @18:28:34.94 PSR.select :air @18:28:34.95 PSV.dial PSR @18:28:36.26 Pulling 2ml of PSR.air @18:28:36.27 PSR.select :air @18:28:36.29 PS.reconfigure :default @18:28:36.33 PS.seek 2ml @18:28:42.88 Pumping wcr puck from tube 3 up to 35psi... @18:28:42.89 CBV.dial SSV @18:28:44.16 CBV.dial between CSV and waste2b @18:28:45.09 CSV.dial between air and CTV @18:28:45.68 CS.seek 7.175ml @18:28:52.69 CTV.dial CSV,avoiding: :air @18:28:52.72 CSV.dial CTV @18:28:54.09 CS.reconfigure :pressurize @18:28:54.13 CS.seek 0ml @18:28:57.49 delay 5 seconds @18:29:02.51 PTV.dial :puck @18:29:02.52 PSV.dial PTV @18:29:03.24 PBV.dial :waste1 @18:29:03.65 PS.seek 1.5ml @18:29:09.61 delay 3 seconds @18:29:10.84 delay 10:00 @18:29:12.63 PBV.dial between PSV and waste1 @18:29:12.99 delay 1 second @18:29:14.01 PSV.dial :waste1 @18:29:15.01 PS.seek 0ml @18:29:31.04 Processing Puck Heater at 27.9C before imaging PH.coast @18:29:31.16 Imaging da puck in Processing Clamp while storing it to tube 2 @18:29:31.32 SC.reconfigure :tight @18:29:31.37 SC.seek 2 @18:29:34.96 SC.configure :limp @18:29:35.65 Elbow.reconfigure :tight @18:29:35.70 Elbow.seek 2 @18:29:37.48 Forearm.reconfigure :tight @18:29:37.52 Forearm.seek 2 + 700 counts @18:29:38.55 SE.reconfigure :pick @18:29:38.60 SE.seek :beamBreak @18:29:41.52 SE.configure :default @18:29:41.67 Elbow.reconfigure :limp @18:29:41.71 Forearm.reconfigure :loose @18:29:41.76 Forearm.seek 2 @18:29:42.16 Elbow.hold @18:29:42.23 Elbow.reconfigure :tight @18:29:42.32 SE.seek 2.19 Pucks below Top Plate @18:29:42.74 Forearm.force -20 @18:29:42.78 Forearm.seek 2 + 317 counts @18:29:43.09 Forearm.reconfigure :tight @18:29:43.14 Forearm.seek 2 + 700 counts @18:29:43.48 SE.seek 3.19 Pucks below Top Plate @18:29:43.95 Forearm.reconfigure :loose @18:29:43.99 Forearm.seek :retract @18:29:44.89 Elbow.seek PC @18:29:47.47 Elbow.reconfigure :loose @18:29:47.56 Forearm.seek PC @18:29:48.68 Hand.close @18:29:49.35 PC.seek :open @18:29:53.37 Forearm.seek :retract @18:29:54.42 Elbow.reconfigure :tight @18:29:54.46 Elbow.seek 2 @18:29:57.18 Forearm.reconfigure :tight @18:29:57.22 Forearm.seek 2 + 700 counts @18:29:58.16 Hand.open @18:29:58.91 SE.seek 2.83 Pucks below Top Plate @18:29:59.19 SE.reconfigure :seal @18:29:59.24 SE.seek 1.73 Pucks below Top Plate @18:29:59.82 Lights, camera, ACTION! @18:29:59.85 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22dec0118h625ml.tif @18:30:19.29 Waiting >20 seconds for da to relinquish FlushPuck @18:35:04.55 WARNING: Too Dark -- required 348.66s exposure > 300s time limit SX MX5/16 (USB:17): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 300 seconds /var/log/esp/da22dec0118h625ml.tif: TIFF Upload Complete @18:35:04.57 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22dec0118h625ml15s.tif @18:35:20.34 SX MX5/16 (USB:17): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22dec0118h625ml15s.tif: TIFF Upload Complete @18:35:20.37 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da22dec0118h625ml25s.tif @18:35:46.15 SX MX5/16 (USB:17): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da22dec0118h625ml25s.tif: TIFF Upload Complete @18:35:46.16 Gate.power :camera,:OFF @18:35:46.19 DAfans.email "300.0 sec auto-exposure",Subject: "da22dec0118h625ml images!" @18:35:46.47 Elbow.seek 2 @18:35:46.83 Forearm.seek 2 + 700 counts @18:35:47.01 SE.reconfigure :gently @18:35:47.07 SE.seek 2.44 Pucks below Top Plate @18:35:47.70 Elbow.reconfigure :limp @18:35:47.75 Forearm.reconfigure :loose @18:35:47.79 Forearm.seek 2 @18:35:48.25 Elbow.hold @18:35:48.31 Elbow.reconfigure :tight @18:35:48.37 SE.reconfigure :default @18:35:48.42 SE.seek 2.63 Pucks below Top Plate @18:35:48.95 Forearm.force -20 @18:35:48.99 Forearm.seek 2 + 312 counts @18:35:49.32 Forearm.reconfigure :tight @18:35:49.36 Forearm.seek 2 + 700 counts @18:35:49.78 SE.seek :down @18:35:51.93 Forearm.reconfigure :loose @18:35:52.05 Forearm.seek :retract @18:35:52.29 SE.stop @18:35:53.07 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:35:53.26 Elbow.seek FlushPuck::Garage @18:35:55.20 Elbow.reconfigure :loose @18:35:55.38 Forearm.seek :garage @18:35:56.50 Hand.close @18:35:57.30 Forearm.seek :retract @18:35:58.43 Elbow.reconfigure :tight @18:35:58.48 Elbow.seek PC @18:35:59.89 Elbow.reconfigure :loose @18:35:59.98 Forearm.reconfigure :tight @18:36:00.04 Forearm.seek PC @18:36:01.67 PC.seek :closed @18:36:05.69 Processing Clamp onSpring/offSpring for 9/24 samples, on/off torque 1.38 @18:36:05.87 Hand.open @18:36:06.73 Forearm.reconfigure :loose @18:36:06.81 Forearm.seek :retract @18:36:07.90 PSR.select :air @18:36:07.94 PSV.dial PSR @18:36:08.72 Pulling 0.5ml of PSR.flush @18:36:08.74 PSR.select :flush @18:36:08.92 PS.seek 0.5ml @18:36:14.90 delay 5 seconds @18:36:19.93 PSR.select :air @18:36:20.09 PS.seek 2.5ml @18:36:41.21 delay 1 second @18:36:42.25 PRV.dial :waste2,avoiding: :ab2 @18:36:44.10 PTV.dial :PRVmixing @18:36:45.35 PSV.dial :mixloop @18:36:46.44 PS.seek :empty @18:37:12.62 PSR.select :air @18:37:12.65 PSV.dial PSR @18:37:13.93 Pulling 0.5ml of PSR.flush @18:37:13.95 PSR.select :flush @18:37:14.12 PS.seek 0.5ml @18:37:20.14 delay 5 seconds @18:37:25.19 PSR.select :air @18:37:25.38 PS.seek 2.5ml @18:37:46.49 delay 1 second @18:37:47.53 PBV.dial :waste1 @18:37:47.93 PTV.dial :mixing @18:37:48.68 PSV.dial :mixloop @18:37:49.90 PS.seek :empty @18:38:16.26 PSV.dial PSR @18:38:17.26 PSR.select :flush @18:38:17.45 PS.seek 1ml @18:38:28.39 PSR.select :air @18:38:28.57 PS.seek 2.5ml @18:38:33.16 upsync @18:38:44.81 PSV.dial PTV @18:38:45.53 PTV.dial :puck @18:38:46.22 PBV.dial :waste1 @18:38:46.24 PS.seek 0ml @18:39:10.85 CBV.dial :waste2b @18:39:11.18 CS.reconfigure :default @18:39:11.22 CS.seek 0ml @18:39:12.40 PSV.dial PSR @18:39:13.12 PSR.select :flush @18:39:13.24 PS.seek 0.5ml @18:39:13.80 delay 1:00 @18:39:19.09 PSR.select :air @18:39:19.20 PS.seek 2.5ml @18:39:40.50 PSV.dial PTV @18:39:41.22 PBV.dial PSV @18:39:41.75 PS.seek 0ml @18:40:07.87 PSV.dial PBV @18:40:08.33 PS.seek 2.5ml @18:40:13.82 CTV.dial :air,avoiding: :intake @18:40:15.08 Fix #2 of 2 (with RNAlater) for 10:00 @18:40:15.09 CSV.dial CSR @18:40:15.57 Pulling 2ml of CSR.RNAlater @18:40:15.58 CSR.select :RNAlater @18:40:15.69 CS.seek 2ml @18:40:21.86 delay 5 seconds @18:40:26.88 CSR.select :air @18:40:26.99 CS.reconfigure :fast @18:40:27.52 CS.seek 10ml @18:40:34.59 PSV.dial :waste1 @18:40:35.30 PS.seek 0ml @18:40:45.73 Pumping wcr puck from tube 3 up to 35psi... @18:40:45.75 CBV.dial SSV @18:40:47.02 CBV.dial between CSV and waste2b @18:40:47.93 CSV.dial between air and CTV @18:40:48.52 CS.seek 7.078ml @18:40:55.71 CTV.dial CSV,avoiding: :air @18:40:55.73 CSV.dial CTV @18:40:57.05 CS.reconfigure :pressurize @18:40:57.10 CS.seek 0ml @18:41:01.56 PSV.dial PSR @18:41:02.01 PSR.select :air @18:41:02.04 PS.seek 2.5ml @18:41:13.69 delay 10:00 @18:41:28.28 PSV.dial PTV @18:41:28.98 PBV.dial PSV @18:41:29.00 PS.seek 0ml @18:41:55.15 PSV.dial PBV @18:41:55.61 PS.seek 2.5ml @18:42:21.87 PSV.dial :waste1 @18:42:22.58 PS.seek 0ml @18:42:48.80 PSV.dial PSR @18:42:49.37 PSR.select :flush @18:42:49.48 PS.seek 1ml @18:43:00.62 PSR.select :air @18:43:00.73 PS.seek 2.5ml @18:43:16.74 PSV.dial PTV @18:43:17.59 PTV.dial :puck @18:43:17.60 PBV.dial :waste1 @18:43:18.12 PS.seek 0ml @18:43:44.30 PSV.dial PSR @18:43:45.01 PSR.select :flush @18:43:45.12 PS.seek 0.5ml @18:43:51.05 PSR.select :air @18:43:51.17 PS.seek 2.5ml @18:44:12.33 PSV.dial PTV @18:44:13.05 PBV.dial PSV @18:44:13.61 PS.seek 0ml @18:44:39.86 PSV.dial PBV @18:44:40.32 PS.seek 2.5ml @18:45:06.52 PSV.dial :waste1 @18:45:07.22 PS.seek 0ml @18:45:33.33 PSV.dial PSR @18:45:33.89 PSR.select :air @18:45:33.92 PS.seek 2.5ml @18:46:00.08 PSV.dial PTV @18:46:00.80 PBV.dial PSV @18:46:00.82 PS.seek 0ml @18:46:27.02 PSV.dial PBV @18:46:27.59 PS.seek 2.5ml @18:46:53.80 PSV.dial :waste1 @18:46:54.52 PS.seek 0ml @18:47:20.71 Garaging FlushPuck in Processing Clamp @18:47:20.78 Elbow.reconfigure :tight @18:47:20.82 Elbow.seek PC @18:47:20.93 Elbow.reconfigure :loose @18:47:21.02 Forearm.seek PC @18:47:22.09 Hand.close @18:47:22.69 PC.seek :open @18:47:26.66 Forearm.seek :retract @18:47:27.74 Elbow.reconfigure :tight @18:47:27.78 Elbow.seek :garage @18:47:29.09 Elbow.reconfigure :loose @18:47:29.18 Forearm.reconfigure :tight @18:47:29.22 Forearm.seek :garage @18:47:30.31 Hand.open @18:47:31.12 Forearm.reconfigure :loose @18:47:31.16 Forearm.seek :retract @18:51:13.71 CBV.dial :waste2b @18:51:14.03 CS.reconfigure :default @18:51:14.08 CS.seek 0ml @18:51:16.56 delay 1:00 @18:52:16.58 CTV.dial :air,avoiding: :intake @18:52:18.33 Storing wcr puck in Collection Clamp to tube 2 @18:52:18.50 SC.reconfigure :tight @18:52:18.55 SC.seek 2 @18:52:18.66 SC.configure :limp @18:52:18.86 Elbow.reconfigure :tight @18:52:18.91 Elbow.seek 2 @18:52:20.86 Forearm.reconfigure :tight @18:52:20.90 Forearm.seek 2 + 700 counts @18:52:21.95 SE.reconfigure :pick @18:52:22.00 SE.seek :beamBreak @18:52:24.97 SE.configure :default @18:52:25.12 Elbow.reconfigure :limp @18:52:25.17 Forearm.reconfigure :loose @18:52:25.21 Forearm.seek 2 @18:52:25.60 Elbow.hold @18:52:25.67 Elbow.reconfigure :tight @18:52:25.76 SE.seek 3.2 Pucks below Top Plate @18:52:26.22 Forearm.force -20 @18:52:26.26 Forearm.seek 2 + 318 counts @18:52:26.53 Forearm.reconfigure :tight @18:52:26.58 Forearm.seek 2 + 700 counts @18:52:26.81 SE.seek 4.2 Pucks below Top Plate @18:52:27.30 Forearm.reconfigure :loose @18:52:27.35 Forearm.seek :retract @18:52:28.28 Elbow.seek CC @18:52:29.78 Elbow.reconfigure :loose @18:52:29.87 Forearm.seek CC @18:52:30.80 Hand.close @18:52:31.56 CC.seek :open @18:52:35.99 Forearm.seek :retract @18:52:37.01 Elbow.reconfigure :tight @18:52:37.06 Elbow.seek 2 @18:52:38.79 Elbow.reconfigure :limp @18:52:38.84 Forearm.seek 2 @18:52:39.90 Elbow.hold @18:52:39.96 Elbow.reconfigure :tight @18:52:40.01 Hand.open @18:52:40.92 Forearm.force -20 @18:52:40.96 Forearm.seek 2 + 318 counts @18:52:41.25 Forearm.reconfigure :tight @18:52:41.29 Forearm.seek 2 + 700 counts @18:52:41.68 SE.seek :down @18:52:42.81 Forearm.reconfigure :loose @18:52:42.86 Forearm.seek :retract @18:52:44.09 SE.stop @18:52:44.13
Starting finalPurge @18:52:44.14 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:52:44.32 Elbow.seek FlushPuck::Garage @18:52:46.29 Elbow.reconfigure :loose @18:52:46.37 Forearm.seek :garage @18:52:47.49 Hand.close @18:52:48.17 Forearm.seek :retract @18:52:49.29 Elbow.reconfigure :tight @18:52:49.33 Elbow.seek CC @18:52:52.09 Elbow.reconfigure :loose @18:52:52.18 Forearm.reconfigure :tight @18:52:52.22 Forearm.seek CC @18:52:53.95 CC.seek :closed @18:52:58.18 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.89 @18:52:58.25 Hand.open @18:52:58.99 Forearm.reconfigure :loose @18:52:59.03 Forearm.seek :retract @18:53:00.07 Flushing the Collection Valve::Manifold @18:53:00.19 CSV.dial CSR @18:53:00.65 Pulling 5ml of CSR.flush @18:53:00.66 CSR.select :flush @18:53:00.78 CS.seek 5ml @18:53:14.25 delay 5 seconds @18:53:19.26 CSR.select :air @18:53:19.41 CS.reconfigure :fast @18:53:19.45 CS.seek 7.5ml @18:53:25.74 CSV.dial :waste @18:53:27.00 CS.reconfigure :default @18:53:27.04 CS.seek 0ml @18:53:46.77 Flushing lower manifold's waste1 port @18:53:46.89 CSV.dial :flush @18:53:47.32 CS.reconfigure :fast @18:53:47.37 CS.seek 5ml @18:53:58.99 delay 5 seconds @18:54:04.01 CSV.dial :air @18:54:04.50 CS.seek 6.999ml @18:54:09.78 CSV.dial CTV @18:54:09.80
CTV.dial CSV @18:54:11.13 CBV.dial :waste1 @18:54:11.86 CS.seek 0ml @18:54:27.93 Flushing lower manifold's waste2 port @18:54:28.04 CSV.dial :flush @18:54:28.76 CS.seek 5ml @18:54:40.49 delay 5 seconds @18:54:45.51 CSV.dial :air @18:54:46.02 CS.seek 7ml @18:54:51.18 CSV.dial CTV @18:54:51.21
CTV.dial CSV @18:54:51.63 CBV.dial :waste2 @18:54:52.64 CS.seek 0ml @18:55:08.43 Flushing lower manifold's waste2b port @18:55:08.54 CSV.dial :flush @18:55:09.26 CS.seek 5ml @18:55:20.96 delay 5 seconds @18:55:25.98 CSV.dial :air @18:55:26.48 CS.seek 7ml @18:55:31.68 CSV.dial CTV @18:55:31.71
CTV.dial CSV @18:55:32.13 CBV.dial :waste2b @18:55:33.12 CS.seek 0ml @18:55:49.05 Disinfecting external Intake with kill @18:55:49.16 CSV.dial CSR @18:55:49.65 Pulling 8ml of CSR.kill @18:55:49.66 CSR.select :kill @18:55:49.77 CS.seek 8ml @18:56:07.87 delay 5 seconds @18:56:12.88 CSR.select :air @18:56:12.99 CS.seek 10ml @18:56:18.28 CSV.dial CBV @18:56:18.30
CBV.dial CSV @18:56:19.33 CTV.dial :intake @18:56:20.05 Intake.select :open @18:56:20.16 CS.seek 2ml @18:56:38.27 Intake.select :close @18:56:38.37 CTV.dial :air @18:56:39.12 CS.reconfigure :default @18:56:39.16 CS.seek 0ml @18:56:45.05 CSV.dial CSR @18:56:46.09 Pulling 5ml of CSR.flush CSR.select :flush @18:56:46.20 CS.seek 5ml @18:56:59.71 delay 5 seconds @18:57:04.72 CSR.select :air @18:57:04.83 CS.seek 10ml @18:57:18.23 CSV.dial CBV @18:57:19.21 CS.seek 0ml @18:57:45.27 Garaging FlushPuck in Collection Clamp @18:57:45.34 Elbow.reconfigure :tight @18:57:45.38 Elbow.seek CC @18:57:45.49 Elbow.reconfigure :loose @18:57:45.58 Forearm.seek CC @18:57:46.54 Hand.close @18:57:47.17 CC.seek :open @18:57:51.50 Forearm.seek :retract @18:57:52.56 Elbow.reconfigure :tight @18:57:52.60 Elbow.seek :garage @18:57:55.92 Elbow.reconfigure :loose @18:57:56.01 Forearm.reconfigure :tight @18:57:56.05 Forearm.seek :garage @18:57:57.15 Hand.open @18:57:57.98 Forearm.reconfigure :loose @18:57:58.02 Forearm.seek :retract @18:57:59.06 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 16:11:57 @18:57:59.11 Asleep after completing DA_WCR_120122 assays @18:57:59.12 GoodNews.email "{1 => 32,\n 2 => 5,\n 3 => 29,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@18:51:00, 23.1C, 34% humidity, 15.7psia, 14.046V, 0.331A, 0.324A avg, 4.64W\nSampled 1000.0ml",Subject: "Asleep after completing DA_WCR_120122 assays" @18:58:34.10
Uploading Logs @19:00:03.06 upsync @23:32:53.54UTC08-Dec-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @23:32:56.10 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @23:33:00.05 Polling Can every 10:00 @23:33:00.11 Cleanly exited at 23:30:36UTC08-Dec-22 @23:33:00.15 Loading /home/esp/esp2/mission/DA_WCR_120822.rb mission @23:33:00.20 Preparing to sleep until + 00:00:05 @23:33:00.21 GoodNews.email "Starting DA_WCR_120822 w/pucks in tube 3\n{1 => 33,\n 2 => 7,\n 3 => 26,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@23:33:00, 22.3C, 35% humidity, 15.6psia, 14.060V, 0.245A, 0.245A avg, 3.44W",Subject: "DA_WCR_120822 begun" @23:33:00.27
Asleep til + 00:00:05 @23:33:00.29 Continuing at 23:33 @23:33:00.54 Gate.power :core,:OFF @23:33:00.55 Gate.power :raw,:OFF @23:33:00.56 Shutdown @23:33:05.17
Powering core bus @23:33:05.26 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_120822.rb:14\n{1 => 33,\n 2 => 7,\n 3 => 26,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@23:33:00, 22.3C, 35% humidity, 15.6psia, 14.060V, 0.245A, 0.245A avg, 3.44W",Subject: "Asleep until 23:33" @23:33:10.85
Gate.power :raw,:ON @23:33:11.98 CS.configure :default @23:33:12.19 PS.configure :default @23:33:12.38 SS.configure :default @23:33:12.54 CC.configure :default @23:33:12.71 PC.configure :default @23:33:12.93 Forearm.configure :loose @23:33:13.11 Elbow.configure :loose @23:33:13.41 SC.configure :loose @23:33:13.59 SE.configure :default @23:33:13.67 CH.coast @23:33:13.79 CH.configure :default @23:33:13.85 PH.coast @23:33:13.95 PH.configure :default @23:33:14.03 All dwarf controllers running firmware version 3.85 @23:33:14.17 Valve.reset @23:33:14.66 Intake.select :close @23:33:14.70 Exhaust.select :close @23:33:14.75 CTV.dial :air @23:33:14.93 PSV.dial :air @23:33:15.05 CSV.dial :waste1 @23:33:15.29
Forearm.reconfigure :loose @23:33:15.37 Forearm.home.seek :retract @23:33:15.63 SSV.dial :spit,avoiding: :exhaust @23:33:16.11 PS.reconfigure :default @23:33:16.21 PS.home.seek 0ml @23:33:16.33 Hand.close @23:33:16.39
Elbow.jog 1500 @23:33:16.61 CS.reconfigure :fast @23:33:16.67 SS.reconfigure :default @23:33:16.81 CS.home.seek 0ml @23:33:16.87 SS.home.seek 0ml @23:33:17.11
Elbow.reconfigure :loose @23:33:17.22 Elbow.home.seek :clear @23:33:21.82 SE.reconfigure :default @23:33:21.90 SE.home.seek :down @23:33:22.55 SC.reconfigure :tight @23:33:22.64 SC.home.seek :initial @23:33:24.91 PS.stop @23:33:52.74 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @23:33:52.76
Retry #1 of 3 @23:33:52.88 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @23:33:57.13 SC.configure :limp @23:33:57.22 Forearm.seek :retract @23:33:57.34 Elbow.seek :hammer @23:33:58.76 Forearm.reconfigure :tight @23:33:58.82 Forearm.seek retracted @23:34:00.30 PC.reconfigure :default @23:34:00.36
CC.reconfigure :default @23:34:00.50 PC.home.seek :open @23:34:00.56
CC.home.seek :open @23:34:04.47 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @23:34:04.49 Notice.email "At 23:34:04 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_120822.rb:14\n{1 => 33,\n 2 => 7,\n 3 => 26,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@23:34:04, 22.3C, 35% humidity, 15.6psia, 14.046V, 0.239A, 0.239A avg, 3.35W",Subject: "Started DA_WCR_120822 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @23:34:04.53
Starting initialPurge @23:34:04.58 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @23:34:04.69 Hand.open @23:34:05.79 Elbow.reconfigure :tight @23:34:05.91 Elbow.seek FlushPuck::Garage @23:34:07.87 Elbow.reconfigure :loose @23:34:08.06 Forearm.reconfigure :loose @23:34:08.12 Forearm.seek :garage @23:34:09.23 Hand.close @23:34:10.13 Forearm.seek :retract @23:34:11.20 Elbow.reconfigure :tight @23:34:11.27 Elbow.seek CC @23:34:14.33 Elbow.reconfigure :loose @23:34:14.54 Forearm.reconfigure :tight @23:34:14.65 Forearm.seek CC @23:34:16.27 CC.seek :closed @23:34:20.63 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.80 @23:34:20.78 Hand.open @23:34:21.65 Forearm.reconfigure :loose @23:34:21.71 Forearm.seek :retract @23:34:22.81 Flushing the Collection Valve::Manifold @23:34:23.05 CSV.dial CSR @23:34:24.45 Pulling 5ml of CSR.flush @23:34:24.47 CSR.select :flush @23:34:24.67 CS.reconfigure :default @23:34:24.75 CS.seek 5ml @23:34:38.19 delay 5 seconds @23:34:43.22 CSR.select :air @23:34:43.45 CS.reconfigure :fast @23:34:43.53 CS.seek 7.5ml @23:34:49.97 CSV.dial :waste @23:34:51.39 CS.reconfigure :default @23:34:51.46 CS.seek 0ml @23:35:11.27 Purging external Intake @23:35:11.51 CSV.dial :flush @23:35:12.02 CS.reconfigure :fast @23:35:12.10 CS.seek 10ml @23:35:16.65 upsync @23:35:34.70
delay 5 seconds @23:35:39.72 CSV.dial CBV @23:35:39.75
CBV.dial CSV @23:35:40.48 CTV.dial :intake @23:35:41.23 Intake.select :open @23:35:41.34 CS.seek 0ml @23:36:03.85 Intake.select :close @23:36:03.95 CTV.dial :air @23:36:04.69 Flushing upper manifold @23:36:04.80 CSV.dial :flush @23:36:05.52 CS.seek 5ml @23:36:17.26 delay 5 seconds @23:36:22.28 CSV.dial :air @23:36:22.90 CS.seek 7ml @23:36:28.17 CSV.dial CBV @23:36:28.20
CBV.dial CSV @23:36:29.17 CTV.dial :air @23:36:29.19 CS.seek 0ml @23:36:45.10 delay 7 seconds @23:36:52.13 CS.seek 3ml @23:36:59.60 CSV.dial :waste @23:37:00.05 CS.seek 0ml @23:37:07.55 CSV.dial :flush @23:37:07.99 CS.seek 3ml @23:37:15.35 delay 5 seconds @23:37:20.37 CSV.dial :air @23:37:20.87 CS.seek 5ml @23:37:26.01 CSV.dial :air @23:37:26.03 CS.seek 0ml @23:37:37.57 delay 7 seconds @23:37:44.60 CS.seek 3ml @23:37:51.94 CSV.dial :waste @23:37:52.65 CS.seek 0ml @23:38:00.01 Garaging FlushPuck in Collection Clamp @23:38:00.08 Elbow.reconfigure :tight @23:38:00.12 Elbow.seek CC @23:38:00.23 Elbow.reconfigure :loose @23:38:00.31 Forearm.seek CC @23:38:01.30 Hand.close @23:38:02.00 CC.seek :open @23:38:06.34 Forearm.seek :retract @23:38:07.33 Elbow.reconfigure :tight @23:38:07.37 Elbow.seek :garage @23:38:10.37 Elbow.reconfigure :loose @23:38:10.45 Forearm.reconfigure :tight @23:38:10.50 Forearm.seek :garage @23:38:11.58 Hand.open @23:38:12.78 Forearm.reconfigure :loose @23:38:12.89 Forearm.seek :retract @23:38:13.97 da sampling at most 1000ml, wcr at most 1000ml @23:38:14.00 Loading sh1 puck from tube 3 into Collection Clamp @23:38:14.02 DAfans.email "Started da in tube 3 at 23:38:13UTC08-Dec-22\n{1 => 33,\n 2 => 7,\n 3 => 26,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@23:34:04, 22.3C, 35% humidity, 15.6psia, 14.046V, 0.239A, 0.239A avg, 3.35W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @23:38:14.27
SC.reconfigure :tight @23:38:14.32 SC.seek 3 @23:38:24.19 SC.configure :limp @23:38:24.64 Elbow.reconfigure :tight @23:38:24.72 Elbow.seek 3 @23:38:26.91 Forearm.reconfigure :tight @23:38:26.99 Forearm.seek 3 + 700 counts @23:38:28.11 SE.reconfigure :pick @23:38:28.19 SE.seek :beamBreak @23:38:29.77 SE.configure :default @23:38:30.07 Elbow.seek 3 @23:38:30.47 Forearm.seek 3 + 300 counts @23:38:30.69 Forearm.reconfigure :loose @23:38:30.78 Hand.close @23:38:31.43 SE.seek :down @23:38:31.85
Forearm.seek :retract @23:38:32.65 SE.stop @23:38:32.94
Elbow.seek CC @23:38:34.60 Elbow.reconfigure :loose @23:38:34.80 Forearm.reconfigure :tight @23:38:34.90 Forearm.seek CC @23:38:36.49 CC.seek :closed @23:38:40.90 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.69 @23:38:40.92 Hand.open @23:38:41.83 Forearm.reconfigure :loose @23:38:41.91 Forearm.seek :retract @23:38:42.99 collectSample 1000ml w/bubble point of 24psi @23:38:43.02 Priming 7ml @23:38:43.05 Intake.select :close @23:38:43.07 Exhaust.select :close @23:38:43.09 SS.hold @23:38:43.51 Calibrating pressure and temperature sensors @23:38:43.57 CTV.dial :air @23:38:43.59 SSV.dial :spit,avoiding: :exhaust @23:38:43.63 SS.reconfigure :fast @23:38:43.71 SS.seek 5ml @23:38:46.90 SSV.dial CBV @23:38:46.98
CBV.dial SSV @23:38:48.26 delay 3.8 seconds @23:38:52.30 SSV.dial :spit @23:38:53.62 SS.seek 0ml @23:38:56.19 SSV.dial CBV @23:38:57.59 CTV.dial :intake @23:38:58.41 SSV.dial CBV @23:38:58.47
CBV.dial SSV @23:38:58.49 Allowing 40s for 7.0ml of remaining 7.0ml @23:38:58.51 Intake.select :open @23:39:00.76 Intake @15.8psia, vacuum from 10.0 to 13.0psi @23:39:00.80 SS.reconfigure :inhaling 2.14ml/s @23:39:00.86 SS.seek 7ml @23:39:07.85 Stroke completed -- waiting for pressure to equalize ... @23:39:07.87 SS.reconfigure :exhale @23:39:07.95 SS.hold @23:39:11.88 Intake.select :close @23:39:12.11 SSV.dial :exhaust @23:39:12.91 Exhaust.select :open @23:39:13.07 SS.seek 0ml @23:39:16.99 Exhaust.select :close @23:39:17.13 Sampling 1000.0ml @23:39:17.15 Intake.select :close @23:39:17.17 Exhaust.select :close @23:39:17.19 SS.reconfigure :default @23:39:17.26 SS.hold @23:39:17.62 CTV.dial :intake @23:39:17.66 SSV.dial CBV @23:39:17.70
CBV.dial SSV @23:39:18.60 Allowing 130s for 25.0ml of remaining 1000.0ml @23:39:18.62 Intake.select :open @23:39:20.86 Intake @15.9psia, vacuum from 10.0 to 13.0psi @23:39:20.88 SS.reconfigure :inhaling 2.14ml/s @23:39:20.96 SS.seek 25ml @23:39:26.25 Vacuum too high -- waiting up to 40 seconds @23:39:26.27 SS.reconfigure :exhale @23:39:26.35 SS.hold @23:39:27.13 Intake @12.1psia, vacuum from 6.6 to 9.6psi @23:39:27.17 SS.reconfigure :inhaling 1.93ml/s @23:39:27.25 SS.seek 25ml @23:39:29.88 Vacuum too high -- waiting up to 40 seconds @23:39:29.93 SS.reconfigure :exhale @23:39:30.04 SS.hold @23:39:31.36 Intake @14.7psia, vacuum from 9.2 to 12.2psi @23:39:31.40 SS.reconfigure :inhaling 1.74ml/s @23:39:31.46 SS.seek 25ml @23:39:34.87 Vacuum too high -- waiting up to 40 seconds @23:39:34.89 SS.reconfigure :exhale @23:39:34.97 SS.hold @23:39:35.68 Intake @12.9psia, vacuum from 7.4 to 10.4psi @23:39:35.72 SS.reconfigure :inhaling 1.57ml/s @23:39:35.78 SS.seek 25ml @23:39:38.19 Vacuum too high -- waiting up to 40 seconds @23:39:38.21 SS.reconfigure :exhale @23:39:38.29 SS.hold @23:39:39.60 Intake @14.1psia, vacuum from 8.6 to 11.6psi @23:39:39.62 SS.reconfigure :inhaling 1.41ml/s @23:39:39.70 SS.seek 25ml @23:39:43.39 Vacuum too high -- waiting up to 40 seconds @23:39:43.41 SS.reconfigure :exhale @23:39:43.49 SS.hold @23:39:44.44 Intake @13.7psia, vacuum from 8.2 to 11.2psi @23:39:44.48 SS.reconfigure :inhaling 1.27ml/s @23:39:44.56 SS.seek 25ml @23:39:48.11 Vacuum too high -- waiting up to 40 seconds @23:39:48.13 SS.reconfigure :exhale @23:39:48.19 SS.hold @23:39:49.08 Intake @13.4psia, vacuum from 7.9 to 10.9psi @23:39:49.16 SS.reconfigure :inhaling 1.14ml/s @23:39:49.23 SS.seek 25ml @23:39:53.94 Vacuum too high -- waiting up to 40 seconds @23:39:53.96 SS.reconfigure :exhale @23:39:54.04 SS.hold @23:39:54.98 Intake @13.4psia, vacuum from 7.9 to 10.9psi @23:39:55.02 SS.reconfigure :inhaling 1.03ml/s @23:39:55.08 SS.seek 25ml @23:39:56.22 Stroke completed -- waiting for pressure to equalize ... @23:39:56.24 SS.reconfigure :exhale @23:39:56.32 SS.hold @23:39:56.82 Intake.select :close @23:39:57.07 SSV.dial :exhaust @23:39:57.84 Exhaust.select :open @23:39:58.00 SS.seek 0ml @23:40:07.33 Exhaust.select :close @23:40:07.48 SSV.dial CBV @23:40:07.57
CBV.dial SSV @23:40:08.41 Allowing 130s for 25.0ml of remaining 975.0ml @23:40:08.43 Intake.select :open @23:40:10.67 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:40:10.71 SS.reconfigure :inhaling 1.03ml/s @23:40:10.80 SS.seek 25ml @23:40:22.37 Vacuum too high -- waiting up to 40 seconds @23:40:22.39 SS.reconfigure :exhale @23:40:22.49 SS.hold @23:40:23.05 Intake @13.0psia, vacuum from 7.5 to 10.5psi @23:40:23.09 SS.reconfigure :inhaling 0.93ml/s @23:40:23.15 SS.seek 25ml @23:40:32.30 upsync @23:40:40.57
Stroke completed -- waiting for pressure to equalize ... @23:40:40.58 SS.reconfigure :exhale @23:40:40.63 SS.hold @23:40:42.56 Intake.select :close @23:40:42.70 SSV.dial :exhaust @23:40:43.42 Exhaust.select :open @23:40:43.53 SS.seek 0ml @23:40:52.64 Exhaust.select :close @23:40:52.74 SSV.dial CBV @23:40:52.79
CBV.dial SSV @23:40:53.50 Allowing 130s for 25.0ml of remaining 950.0ml @23:40:53.51 Intake.select :open @23:40:55.66 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:40:55.68 SS.reconfigure :inhaling 0.93ml/s @23:40:55.72 SS.seek 25ml @23:41:06.83 Vacuum too high -- waiting up to 40 seconds @23:41:06.84 SS.reconfigure :exhale @23:41:06.88 SS.hold @23:41:07.26 Intake @13.1psia, vacuum from 7.6 to 10.6psi @23:41:07.28 SS.reconfigure :inhaling 0.84ml/s @23:41:07.32 SS.seek 25ml @23:41:27.51 Stroke completed -- waiting for pressure to equalize ... @23:41:27.52 SS.reconfigure :exhale @23:41:27.56 SS.hold @23:41:29.50 Intake.select :close @23:41:29.64 SSV.dial :exhaust @23:41:30.36 Exhaust.select :open @23:41:30.46 SS.seek 0ml @23:41:39.73 Exhaust.select :close @23:41:39.83 SSV.dial CBV @23:41:39.86
CBV.dial SSV @23:41:40.61 Allowing 130s for 25.0ml of remaining 925.0ml @23:41:40.62 Intake.select :open @23:41:42.77 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:41:42.78 SS.reconfigure :inhaling 0.84ml/s @23:41:42.82 SS.seek 25ml @23:42:07.43 Vacuum too high -- waiting up to 40 seconds @23:42:07.44 SS.reconfigure :exhale @23:42:07.48 SS.hold @23:42:08.13 Intake @13.5psia, vacuum from 8.0 to 11.0psi @23:42:08.15 SS.reconfigure :inhaling 0.76ml/s @23:42:08.19 SS.seek 25ml @23:42:16.84 Stroke completed -- waiting for pressure to equalize ... @23:42:16.85 SS.reconfigure :exhale @23:42:16.89 SS.hold @23:42:19.28 Intake.select :close @23:42:19.42 SSV.dial :exhaust @23:42:20.15 Exhaust.select :open @23:42:20.26 SS.seek 0ml @23:42:29.48 Exhaust.select :close @23:42:29.58 SSV.dial CBV @23:42:29.61
CBV.dial SSV @23:42:30.34 Allowing 130s for 25.0ml of remaining 900.0ml @23:42:30.35 Intake.select :open @23:42:32.50 Intake @15.3psia, vacuum from 9.8 to 12.8psi @23:42:32.52 SS.reconfigure :inhaling 0.76ml/s @23:42:32.56 SS.seek 25ml @23:42:54.20 Vacuum too high -- waiting up to 40 seconds @23:42:54.21 SS.reconfigure :exhale @23:42:54.26 SS.hold @23:42:55.12 Intake @13.9psia, vacuum from 8.4 to 11.4psi @23:42:55.14 SS.reconfigure :inhaling 0.69ml/s @23:42:55.18 SS.seek 25ml @23:43:10.04 Stroke completed -- waiting for pressure to equalize ... @23:43:10.05 SS.reconfigure :exhale @23:43:10.09 SS.hold @23:43:13.06 Intake.select :close @23:43:13.20 SSV.dial :exhaust @23:43:13.93 Exhaust.select :open @23:43:14.04 SS.seek 0ml @23:43:22.87 Exhaust.select :close @23:43:22.97 SSV.dial CBV @23:43:23.02
CBV.dial SSV @23:43:23.73 Allowing 130s for 25.0ml of remaining 875.0ml @23:43:23.74 Intake.select :open @23:43:25.89 Intake @15.4psia, vacuum from 9.9 to 12.9psi @23:43:25.91 SS.reconfigure :inhaling 0.69ml/s @23:43:25.95 SS.seek 25ml @23:43:50.98 Vacuum too high -- waiting up to 40 seconds @23:43:50.99 SS.reconfigure :exhale @23:43:51.03 SS.hold @23:43:51.97 Intake @13.8psia, vacuum from 8.3 to 11.3psi @23:43:51.99 SS.reconfigure :inhaling 0.61ml/s @23:43:52.03 SS.seek 25ml @23:44:07.93 Stroke completed -- waiting for pressure to equalize ... @23:44:07.94 SS.reconfigure :exhale @23:44:07.98 SS.hold @23:44:12.03 Intake.select :close @23:44:12.16 SSV.dial :exhaust @23:44:12.90 Exhaust.select :open @23:44:13.00 SS.seek 0ml @23:44:22.36 Exhaust.select :close @23:44:22.47 SSV.dial CBV @23:44:22.50
CBV.dial SSV @23:44:23.24 Allowing 130s for 25.0ml of remaining 850.0ml @23:44:23.25 Intake.select :open @23:44:25.40 Intake @15.3psia, vacuum from 9.8 to 12.8psi @23:44:25.42 SS.reconfigure :inhaling 0.61ml/s @23:44:25.46 SS.seek 25ml @23:44:48.54 Vacuum too high -- waiting up to 40 seconds @23:44:48.55 SS.reconfigure :exhale @23:44:48.60 SS.hold @23:44:49.54 Intake @13.8psia, vacuum from 8.3 to 11.3psi @23:44:49.56 SS.reconfigure :inhaling 0.56ml/s @23:44:49.60 SS.seek 25ml @23:45:11.39 Stroke completed -- waiting for pressure to equalize ... @23:45:11.40 SS.reconfigure :exhale @23:45:11.44 SS.hold @23:45:16.95 Intake.select :close @23:45:17.09 SSV.dial :exhaust @23:45:17.82 Exhaust.select :open @23:45:17.92 SS.seek 0ml @23:45:27.32 Exhaust.select :close @23:45:27.42 SSV.dial CBV @23:45:27.45
CBV.dial SSV @23:45:28.18 Allowing 130s for 25.0ml of remaining 825.0ml @23:45:28.19 Intake.select :open @23:45:30.34 Intake @15.4psia, vacuum from 9.9 to 12.9psi @23:45:30.36 SS.reconfigure :inhaling 0.56ml/s @23:45:30.40 SS.seek 25ml @23:45:52.51 Vacuum too high -- waiting up to 40 seconds @23:45:52.52 SS.reconfigure :exhale @23:45:52.56 SS.hold @23:45:54.58 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:45:54.60 SS.reconfigure :inhaling 0.50ml/s @23:45:54.64 SS.seek 25ml @23:46:22.56 Stroke completed -- waiting for pressure to equalize ... @23:46:22.57 SS.reconfigure :exhale @23:46:22.62 SS.hold @23:46:29.00 Intake.select :close @23:46:29.14 SSV.dial :exhaust @23:46:29.86 Exhaust.select :open @23:46:29.97 SS.seek 0ml @23:46:39.39 Exhaust.select :close @23:46:39.49 SSV.dial CBV @23:46:39.52
CBV.dial SSV @23:46:40.27 Allowing 130s for 25.0ml of remaining 800.0ml @23:46:40.28 Intake.select :open @23:46:42.43 Intake @15.3psia, vacuum from 9.8 to 12.8psi @23:46:42.45 SS.reconfigure :inhaling 0.50ml/s @23:46:42.49 SS.seek 25ml @23:47:10.07 Vacuum too high -- waiting up to 40 seconds @23:47:10.08 SS.reconfigure :exhale @23:47:10.12 SS.hold @23:47:13.28 Intake @15.4psia, vacuum from 9.9 to 12.9psi @23:47:13.30 SS.reconfigure :inhaling 0.46ml/s @23:47:13.34 SS.seek 25ml @23:47:42.36 Stroke completed -- waiting for pressure to equalize ... @23:47:42.37 SS.reconfigure :exhale @23:47:42.41 SS.hold @23:47:50.86 Intake.select :close @23:47:51.00 SSV.dial :exhaust @23:47:51.73 Exhaust.select :open @23:47:51.84 SS.seek 0ml @23:48:01.26 Exhaust.select :close @23:48:01.37 SSV.dial CBV @23:48:01.39
CBV.dial SSV @23:48:02.14 Allowing 130s for 25.0ml of remaining 775.0ml @23:48:02.15 Intake.select :open @23:48:04.30 Intake @15.3psia, vacuum from 9.8 to 12.8psi @23:48:04.32 SS.reconfigure :inhaling 0.46ml/s @23:48:04.36 SS.seek 25ml @23:48:32.07 Vacuum too high -- waiting up to 40 seconds @23:48:32.08 SS.reconfigure :exhale @23:48:32.12 SS.hold @23:48:35.57 Intake @15.4psia, vacuum from 9.9 to 12.9psi @23:48:35.59 SS.reconfigure :inhaling 0.41ml/s @23:48:35.63 SS.seek 25ml @23:49:08.00 Vacuum too high -- waiting up to 40 seconds @23:49:08.01 SS.reconfigure :exhale @23:49:08.06 SS.hold @23:49:12.78 SS.reconfigure :inhaling 0.37ml/s @23:49:12.83 SS.seek 25ml @23:49:17.06 Stroke completed -- waiting for pressure to equalize ... @23:49:17.07 SS.reconfigure :exhale @23:49:17.11 SS.hold @23:49:22.62 Intake.select :close @23:49:22.76 SSV.dial :exhaust @23:49:23.48 Exhaust.select :open @23:49:23.59 SS.seek 0ml @23:49:32.70 Exhaust.select :close @23:49:32.80 SSV.dial CBV @23:49:32.85
CBV.dial SSV @23:49:33.54 Allowing 130s for 25.0ml of remaining 750.0ml @23:49:33.56 Intake.select :open @23:49:35.70 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:49:35.72 SS.reconfigure :inhaling 0.37ml/s @23:49:35.76 SS.seek 25ml @23:50:33.78 Vacuum too high -- waiting up to 40 seconds @23:50:33.79 SS.reconfigure :exhale @23:50:33.83 SS.hold @23:50:39.43 Intake @15.1psia, vacuum from 9.6 to 12.6psi @23:50:39.45 SS.reconfigure :inhaling 0.33ml/s @23:50:39.49 SS.seek 25ml @23:50:59.69 Stroke completed -- waiting for pressure to equalize ... @23:50:59.70 SS.reconfigure :exhale @23:50:59.74 SS.hold @23:51:10.74 Intake.select :close @23:51:10.88 SSV.dial :exhaust @23:51:11.60 Exhaust.select :open @23:51:11.71 SS.seek 0ml @23:51:21.48 Exhaust.select :close @23:51:21.58 SSV.dial CBV @23:51:21.61
CBV.dial SSV @23:51:22.36 Allowing 130s for 25.0ml of remaining 725.0ml @23:51:22.37 Intake.select :open @23:51:24.52 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:51:24.53 SS.reconfigure :inhaling 0.33ml/s @23:51:24.58 SS.seek 25ml @23:52:29.75 Vacuum too high -- waiting up to 40 seconds @23:52:29.76 SS.reconfigure :exhale @23:52:29.80 SS.hold @23:52:37.14 SS.reconfigure :inhaling 0.30ml/s @23:52:37.18 SS.seek 25ml @23:53:00.99 Stroke completed -- waiting for pressure to equalize ... @23:53:01.00 SS.reconfigure :exhale @23:53:01.04 SS.hold @23:53:13.58 Intake.select :close @23:53:13.71 SSV.dial :exhaust @23:53:14.44 Exhaust.select :open @23:53:14.55 SS.seek 0ml @23:53:23.92 Exhaust.select :close @23:53:24.03 SSV.dial CBV @23:53:24.05
CBV.dial SSV @23:53:24.80 Allowing 130s for 25.0ml of remaining 700.0ml @23:53:24.81 Intake.select :open @23:53:26.96 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:53:26.98 SS.reconfigure :inhaling 0.30ml/s @23:53:27.02 SS.seek 25ml @23:54:39.52 Vacuum too high -- waiting up to 40 seconds @23:54:39.53 SS.reconfigure :exhale @23:54:39.57 SS.hold @23:54:49.29 Intake @15.7psia, vacuum from 10.0 to 13.0psi @23:54:49.31 SS.reconfigure :inhaling 0.27ml/s @23:54:49.35 SS.seek 25ml @23:55:15.78 Stroke completed -- waiting for pressure to equalize ... @23:55:15.79 SS.reconfigure :exhale @23:55:15.83 SS.hold @23:55:32.09 Intake.select :close @23:55:32.23 SSV.dial :exhaust @23:55:32.95 Exhaust.select :open @23:55:33.06 SS.seek 0ml @23:55:42.50 Exhaust.select :close @23:55:42.60 SSV.dial CBV @23:55:42.64
CBV.dial SSV @23:55:43.36 Allowing 130s for 25.0ml of remaining 675.0ml @23:55:43.37 Intake.select :open @23:55:45.52 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:55:45.54 SS.reconfigure :inhaling 0.27ml/s @23:55:45.58 SS.seek 25ml @23:56:52.33 Vacuum too high -- waiting up to 40 seconds @23:56:52.34 SS.reconfigure :exhale @23:56:52.38 SS.hold @23:57:01.25 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:57:01.27 SS.reconfigure :inhaling 0.24ml/s @23:57:01.31 SS.seek 25ml @23:57:47.54 Stroke completed -- waiting for pressure to equalize ... @23:57:47.55 SS.reconfigure :exhale @23:57:47.60 SS.hold @23:57:53.38 SS.reconfigure :default @23:57:53.42 SS.hold @23:57:53.52 Intake.select :close @23:57:53.66 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 350.0ml @23:57:53.67 SSV.dial :exhaust @23:57:54.40 Exhaust.select :open @23:57:54.51 SS.reconfigure :exhale @23:57:54.55 SS.seek 0ml @23:58:04.02 Exhaust.select :close @23:58:04.13 Sampled 350.0ml @23:58:04.14 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @23:58:04.15 Intake.select :close @23:58:04.20 CSV.dial :waste @23:58:04.27 CS.seek 0ml @23:58:04.34 SSV.dial CBV,avoiding: :exhaust @23:58:04.37 CSV.dial :air @23:58:04.39
CBV.dial SSV @23:58:05.14 CS.seek 10ml @23:58:06.22
CTV.dial :air @23:58:06.96 delay 15 seconds @23:58:21.98 SSV.dial :spit,avoiding: :exhaust @23:58:23.30 CTV.dial CSV,avoiding: :intake @23:58:23.32
SS.seek 0ml @23:58:23.44 SSV.dial CBV,avoiding: :exhaust @23:58:27.93 CBV.dial between SSV and waste2 @23:58:28.31 CTV.dial CSV,avoiding: :air @23:58:28.33
CSV.dial CTV @23:58:28.78 CS.reconfigure :pressurize @23:58:28.82 CS.seek 0ml @23:58:47.25 BubblePoint: Intake @40.2psia, Outlet @16.0psia @23:58:47.26 CBV.dial SSV @23:58:47.59 SS.reconfigure :default @23:58:47.63 SS.seek 10ml @23:58:53.07 delay 15 seconds @23:59:08.18 Evacuated: Intake @16.1psia, Outlet @10.4psia @23:59:08.19 SSV.dial :spit,avoiding: :exhaust @23:59:09.48 SS.reconfigure :exhale @23:59:09.52 SS.seek 0ml @23:59:14.95 SS.reconfigure :fast @23:59:14.99 SS.seek 5ml @23:59:17.86 SSV.dial CBV,avoiding: :exhaust @23:59:17.88
CBV.dial SSV @23:59:19.15 CTV.dial :air,avoiding: :intake @23:59:20.41 CSV.dial CTV @23:59:20.45
SS.seek 10ml @23:59:21.45 CSV.dial :air @23:59:21.90 CS.reconfigure :fast @23:59:21.94 CS.seek 0ml @23:59:23.26
SSV.dial :spit,avoiding: :exhaust @23:59:24.56 SS.reconfigure :exhale @23:59:24.60 SS.seek 0ml @23:59:29.03 CS.stop @23:59:29.63
SS.reconfigure :fast @23:59:29.67 SS.seek 5ml @23:59:32.73 SSV.dial CBV,avoiding: :exhaust @23:59:32.78
CBV.dial SSV @23:59:35.03 SSV.dial :spit,avoiding: :exhaust @23:59:36.32 SS.reconfigure :default @23:59:36.37 SS.seek 0ml @23:59:39.46 SS.stop @23:59:39.49 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @23:59:39.50 Intake.select :close @23:59:39.57 CSV.dial :waste @23:59:39.68 SSV.dial CBV,avoiding: :exhaust @23:59:39.71
CBV.dial SSV @23:59:40.32 CS.seek 0ml @23:59:40.38 CSV.dial :air @23:59:40.99
CTV.dial :air @23:59:41.01 delay 15 seconds @23:59:41.50 CS.seek 10ml @23:59:56.46
SSV.dial :spit,avoiding: :exhaust @23:59:57.75 CTV.dial CSV,avoiding: :intake @23:59:57.77
SS.reconfigure :exhale @23:59:57.87 SS.seek 0ml @23:59:57.93 SSV.dial CBV,avoiding: :exhaust @00:00:04.12UTC09-Dec-22 CBV.dial between SSV and waste2 @00:00:04.50 CTV.dial CSV,avoiding: :air @00:00:04.53
CSV.dial CTV @00:00:04.98 CS.reconfigure :pressurize @00:00:05.02 CS.seek 0ml @00:00:26.25 BubblePoint: Intake @39.3psia, Outlet @15.2psia @00:00:26.26 CBV.dial SSV @00:00:26.58 SS.reconfigure :default @00:00:26.63 SS.seek 10ml @00:00:32.38 delay 15 seconds @00:00:47.49 Evacuated: Intake @18.3psia, Outlet @12.2psia @00:00:47.50 SSV.dial :spit,avoiding: :exhaust @00:00:48.81 SS.reconfigure :exhale @00:00:48.85 SS.seek 0ml @00:00:54.01 SS.reconfigure :fast @00:00:54.05 SS.seek 5ml @00:00:56.85 SSV.dial CBV,avoiding: :exhaust @00:00:56.88
CBV.dial SSV @00:00:58.16 CTV.dial :air,avoiding: :intake @00:00:59.42 CSV.dial CTV @00:00:59.46
SS.seek 10ml @00:01:00.46 CSV.dial :air @00:01:01.02 CS.reconfigure :fast @00:01:01.06 CS.seek 0ml @00:01:01.94
SSV.dial :spit,avoiding: :exhaust @00:01:03.24 SS.reconfigure :exhale @00:01:03.29 SS.seek 0ml @00:01:05.81 CS.stop @00:01:08.53
SS.reconfigure :fast @00:01:08.57 SS.seek 5ml @00:01:11.34 SSV.dial CBV,avoiding: :exhaust @00:01:11.37
CBV.dial SSV @00:01:13.64 SSV.dial :spit,avoiding: :exhaust @00:01:14.92 SS.reconfigure :default @00:01:14.96 SS.seek 0ml @00:01:18.24 SS.stop @00:01:18.27 Lysing: Adding 2.0ml AqMeOH @00:01:18.28 CBV.dial between CSV and waste1,avoiding: :DBV @00:01:19.72 CTV.dial CSV @00:01:20.98 CSV.dial CSR @00:01:21.85 Pulling 1ml of CSR.AqMeOH @00:01:21.86 CSR.select :AqMeOH @00:01:21.97 CS.reconfigure :default @00:01:22.01 CS.seek 1ml @00:01:25.31 delay 5 seconds @00:01:30.33 CSR.select :air @00:01:30.48 CS.seek 6ml @00:01:43.84 delay 2 seconds @00:01:45.86 CSV.dial :waste2 @00:01:46.30 CS.reconfigure :fast @00:01:46.34 CS.seek 0ml @00:02:00.09 CSV.dial CSR @00:02:00.57 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @00:02:00.68 CS.reconfigure :default @00:02:00.72 CS.seek 2ml @00:02:06.59 delay 5 seconds @00:02:11.61 CSR.select :air @00:02:11.76 CS.seek 4.999ml @00:02:20.06 delay 2 seconds @00:02:22.08 CSV.dial CTV @00:02:22.50 CS.seek 1ml @00:02:33.57 Before lysing Collection Puck Heater at 17.9C/38.8psia @00:02:33.59 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @00:02:33.61 CH.seek 70C,8:00 @00:04:17.66 CH.hold 10:00,70C @00:04:17.84 Begun lysing Collection Puck Heater at 68C/41.0psia @00:14:17.85 Ended lysing Collection Puck Heater at 70C/42.0psia @00:14:17.86 CS.seek 0ml @00:14:21.20 CTV.dial between spare and air,avoiding: :intake @00:14:22.18 CSV.dial :air @00:14:22.63 CS.seek 5ml @00:14:36.10 CSV.dial CTV @00:14:36.65 delay 2 seconds @00:14:38.67 CSV.dial :air @00:14:39.10 delay 2 seconds @00:14:41.12 CSV.dial CTV @00:14:41.57 delay 2 seconds @00:14:43.59 CSV.dial :air @00:14:44.04 delay 2 seconds @00:14:46.06 CS.seek 0ml @00:14:59.50 Puck @65.6psia after final lysis pressurization @00:14:59.51 Recovering Lysate into Collection syringe @00:14:59.52 CSV.dial CBV @00:15:00.62 CBV.dial CSV @00:15:00.97 CS.seek 5.001ml @00:15:14.35 delay 5 seconds @00:15:19.37 CTV.dial :air @00:15:19.88 CS.seek 7.001ml @00:15:25.82 delay 5 seconds @00:15:30.84 CSV.dial :air2 @00:15:31.30 CS.seek 10ml @00:15:39.63 CSV.dial between air and CTV,avoiding: CBV @00:15:40.75 Lysing Complete @00:15:40.81 Storing sh1 puck in Collection Clamp to tube 2 @00:15:40.97 SC.reconfigure :tight @00:15:41.01 SC.seek 2 @00:15:44.58 SC.configure :limp @00:15:44.78 Elbow.reconfigure :tight @00:15:44.82 Elbow.seek 2 @00:15:46.52 Forearm.reconfigure :tight @00:15:46.56 Forearm.seek 2 + 700 counts @00:15:47.51 SE.reconfigure :pick @00:15:47.56 SE.seek :beamBreak @00:15:50.32 SE.configure :default @00:15:50.46 Elbow.reconfigure :limp @00:15:50.50 Forearm.reconfigure :loose @00:15:50.54 Forearm.seek 2 @00:15:50.95 Elbow.hold @00:15:51.01 Elbow.reconfigure :tight @00:15:51.10 SE.seek 6.23 Pucks below Top Plate @00:15:51.58 Forearm.force -20 @00:15:51.61 Forearm.seek 2 + 305 counts @00:15:51.94 Forearm.reconfigure :tight @00:15:51.98 Forearm.seek 2 + 700 counts @00:15:52.40 SE.seek 7.23 Pucks below Top Plate @00:15:52.90 Forearm.reconfigure :loose @00:15:52.94 Forearm.seek :retract @00:15:53.97 Elbow.seek CC @00:15:55.51 Elbow.reconfigure :loose @00:15:55.60 Forearm.seek CC @00:15:56.61 Hand.close @00:15:57.18 CC.seek :open @00:16:01.59 Forearm.seek :retract @00:16:02.65 Elbow.reconfigure :tight @00:16:02.69 Elbow.seek 2 @00:16:04.95 Elbow.reconfigure :limp @00:16:05.00 Forearm.seek 2 @00:16:06.10 Elbow.hold @00:16:06.16 Elbow.reconfigure :tight @00:16:06.20 Hand.open @00:16:07.04 Forearm.force -20 @00:16:07.08 Forearm.seek 2 + 318 counts @00:16:07.38 Forearm.reconfigure :tight @00:16:07.42 Forearm.seek 2 + 700 counts @00:16:07.77 SE.seek :down @00:16:08.90
Forearm.reconfigure :loose @00:16:08.94 Forearm.seek :retract @00:16:09.97 SE.stop @00:16:10.01
Tranferring DA @00:16:10.02 Beginning samplePassC @00:16:10.03 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:16:10.21 Elbow.seek FlushPuck::Garage @00:16:12.17 Elbow.reconfigure :loose @00:16:12.26 Forearm.seek :garage @00:16:13.38 Hand.close @00:16:14.03 Forearm.seek :retract @00:16:15.18 Elbow.reconfigure :tight @00:16:15.22 Elbow.seek CC @00:16:18.01 Elbow.reconfigure :loose @00:16:18.10 Forearm.reconfigure :tight @00:16:18.14 Forearm.seek CC @00:16:19.42 CC.seek :closed @00:16:23.65 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.89 @00:16:23.72 Hand.open @00:16:24.48 Forearm.reconfigure :loose @00:16:24.52 Forearm.seek :retract @00:16:25.55 PSV.dial PSR @00:16:26.86 Pulling 1.5ml of PSR.flush @00:16:26.87 PSR.select :flush @00:16:26.98 PS.seek 1.5ml @00:16:43.22 delay 5 seconds @00:16:48.23 PSV.dial :waste1 @00:16:48.67 PS.seek 0ml @00:17:04.68 PSV.dial PSR @00:17:05.23 PSR.select :air @00:17:05.34 PS.seek 2.5ml @00:17:31.47 PRV.dial CBV,avoiding: :ab2 @00:17:31.49
CBV.dial PRV @00:17:33.12 PSV.dial :mixloop @00:17:34.25 CTV.dial :air,avoiding: :intake @00:17:34.26 PTV.dial :PRVmixing @00:17:35.48 PS.seek 0ml @00:18:01.68 PSV.dial :air @00:18:02.12 PS.seek 2.5ml @00:18:28.27 PSV.dial :mixloop @00:18:28.72 PS.seek 0ml @00:18:54.96 PSV.dial :air @00:18:55.40 PS.seek 2.5ml @00:19:21.58 PSV.dial :mixloop @00:19:22.03 PS.seek 0ml @00:19:48.15 Garaging FlushPuck in Collection Clamp @00:19:48.22 Elbow.reconfigure :tight @00:19:48.26 Elbow.seek CC @00:19:48.36 Elbow.reconfigure :loose @00:19:48.44 Forearm.seek CC @00:19:49.51 Hand.close @00:19:50.17 CC.seek :open @00:19:54.48 Forearm.seek :retract @00:19:55.50 Elbow.reconfigure :tight @00:19:55.54 Elbow.seek :garage @00:19:58.41 Elbow.reconfigure :loose @00:19:58.96 Forearm.reconfigure :tight @00:19:59.01 Forearm.seek :garage @00:20:00.09 Hand.open @00:20:00.85 Forearm.reconfigure :loose @00:20:00.89 Forearm.seek :retract @00:20:01.93 PSV.dial :mixloop @00:20:01.94 PTV.dial :PRVmixing @00:20:01.96 PRV.dial CBV @00:20:01.98
CBV.dial PRV @00:20:02.00 CTV.dial :air,avoiding: :intake @00:20:02.01 Loading lyfil puck from tube 3 into Collection Clamp @00:20:02.23 SC.reconfigure :tight @00:20:02.27 SC.seek 3 @00:20:05.86 SC.configure :limp @00:20:06.05 Elbow.reconfigure :tight @00:20:06.09 Elbow.seek 3 @00:20:08.07 Forearm.reconfigure :tight @00:20:08.12 Forearm.seek 3 + 700 counts @00:20:09.18 SE.reconfigure :pick @00:20:09.22 SE.seek :beamBreak @00:20:10.69 SE.configure :default @00:20:10.83 Elbow.seek 3 @00:20:11.00 Forearm.seek 3 + 300 counts @00:20:11.31 Forearm.reconfigure :loose @00:20:11.35 Hand.close @00:20:11.93 SE.seek :down @00:20:12.11
Forearm.seek :retract @00:20:13.04 Elbow.seek CC @00:20:13.11 SE.stop @00:20:14.55
Elbow.reconfigure :loose @00:20:14.64 Forearm.reconfigure :tight @00:20:14.68 Forearm.seek CC @00:20:15.94 CC.seek :closed @00:20:20.13 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.67 @00:20:20.15 Hand.open @00:20:20.98 Forearm.reconfigure :loose @00:20:21.02 Forearm.seek :retract @00:20:22.10 CTV.dial CSV,avoiding: :intake @00:20:23.37 CSV.dial CTV @00:20:23.79 PS.seek 2.5ml @00:20:50.02 CS.seek 5.499ml @00:21:02.25 delay 8 seconds @00:21:10.27 PSV.dial :air @00:21:10.71 delay 2 seconds @00:21:12.72 PSV.dial :waste2 @00:21:13.45 PS.seek 0ml @00:21:39.61 CBV.dial between PRV and waste1 @00:21:39.94 CSV.dial :air @00:21:40.49 CS.seek 10ml @00:21:52.59 CSV.dial CTV @00:21:53.00 CS.seek 0ml @00:22:19.06 CBV.dial :waste1 @00:22:19.36 delay 5 seconds @00:22:24.38 CTV.dial :air,avoiding: :intake @00:22:25.73 Storing lyfil puck in Collection Clamp to tube 2 @00:22:25.88 SC.reconfigure :tight @00:22:25.92 SC.seek 2 @00:22:29.48 SC.configure :limp @00:22:29.67 Elbow.reconfigure :tight @00:22:29.71 Elbow.seek 2 @00:22:31.63 Forearm.reconfigure :tight @00:22:31.67 Forearm.seek 2 + 700 counts @00:22:32.70 SE.reconfigure :pick @00:22:33.22 SE.seek :beamBreak @00:22:35.87 SE.configure :default @00:22:36.01 Elbow.reconfigure :limp @00:22:36.06 Forearm.reconfigure :loose @00:22:36.10 Forearm.seek 2 @00:22:36.49 Elbow.hold @00:22:36.55 Elbow.reconfigure :tight @00:22:36.64 SE.seek 7.23 Pucks below Top Plate @00:22:37.09 Forearm.force -20 @00:22:37.13 Forearm.seek 2 + 314 counts @00:22:37.49 Forearm.reconfigure :tight @00:22:37.54 Forearm.seek 2 + 700 counts @00:22:37.84 SE.seek 8.23 Pucks below Top Plate @00:22:38.41 Forearm.reconfigure :loose @00:22:38.45 Forearm.seek :retract @00:22:39.44 Elbow.seek CC @00:22:41.03 Elbow.reconfigure :loose @00:22:41.12 Forearm.seek CC @00:22:42.13 Hand.close @00:22:42.91 CC.seek :open @00:22:47.33 Forearm.seek :retract @00:22:48.32 Elbow.reconfigure :tight @00:22:48.36 Elbow.seek 2 @00:22:50.12 Elbow.reconfigure :limp @00:22:50.17 Forearm.seek 2 @00:22:51.28 Elbow.hold @00:22:51.34 Elbow.reconfigure :tight @00:22:51.39 Hand.open @00:22:52.28 Forearm.force -20 @00:22:52.32 Forearm.seek 2 + 320 counts @00:22:52.60 Forearm.reconfigure :tight @00:22:52.65 Forearm.seek 2 + 700 counts @00:22:53.13 SE.seek :down @00:22:54.25
Forearm.reconfigure :loose @00:22:54.29 Forearm.seek :retract @00:22:55.37 SE.stop @00:22:55.40
PSV.dial :air @00:22:56.12 PS.seek 2.5ml @00:23:22.33 delay 1 second @00:23:23.34 PSV.dial :mixloop @00:23:23.77 delay 1 second @00:23:24.78 PSV.dial :air @00:23:25.22 delay 1 second @00:23:26.23 PSV.dial :mixloop @00:23:26.67 delay 1 second @00:23:27.69 PSV.dial :air @00:23:28.13 delay 1 second @00:23:29.15 PS.seek 0ml @00:23:55.33 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @00:23:55.50 Elbow.seek FlushPuck::Garage @00:23:57.32 Elbow.reconfigure :loose @00:23:57.41 Forearm.seek :garage @00:23:58.50 Hand.close @00:23:59.27 Forearm.seek :retract @00:24:00.40 Elbow.reconfigure :tight @00:24:00.44 Elbow.seek PC @00:24:01.73 Elbow.reconfigure :loose @00:24:01.82 Forearm.reconfigure :tight @00:24:01.86 Forearm.seek PC @00:24:03.15 PC.seek :closed @00:24:07.01 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.42 @00:24:07.08 Hand.open @00:24:08.31 Forearm.reconfigure :loose @00:24:08.35 Forearm.seek :retract @00:24:09.37 PRV.dial :air @00:24:09.81 PSV.dial :mixloop @00:24:10.33 PS.seek 0.3ml @00:24:14.26 PSV.dial PSR @00:24:15.29 Pulling 0.4ml of PSR.air PSR.select :air @00:24:15.32 PS.seek 0.7ml @00:24:20.20 delay 3 seconds @00:24:23.22 PSR.select :air @00:24:23.24 PS.seek 2.5ml @00:24:42.33 delay 3 seconds @00:24:45.34 PSV.dial :mixloop @00:24:46.33 PTV.dial :mixing @00:24:47.04 PBV.dial :waste2 @00:24:48.03 PS.seek 0ml @00:25:14.26 Garaging FlushPuck in Processing Clamp @00:25:14.33 Elbow.reconfigure :tight @00:25:14.37 Elbow.seek PC @00:25:14.47 Elbow.reconfigure :loose @00:25:14.56 Forearm.seek PC @00:25:15.61 Hand.close @00:25:16.16 PC.seek :open @00:25:20.12 Forearm.seek :retract @00:25:21.16 Elbow.reconfigure :tight @00:25:21.20 Elbow.seek :garage @00:25:22.57 Elbow.reconfigure :loose @00:25:22.66 Forearm.reconfigure :tight @00:25:22.70 Forearm.seek :garage @00:25:23.78 Hand.open @00:25:24.92 Forearm.reconfigure :loose @00:25:24.96 Forearm.seek :retract @00:25:26.08 Loading da puck from tube 3 into Processing Clamp @00:25:26.29 SC.reconfigure :tight @00:25:26.33 SC.seek 3 @00:25:29.91 SC.configure :limp @00:25:30.10 Elbow.reconfigure :tight @00:25:30.15 Elbow.seek 3 @00:25:32.01 Forearm.reconfigure :tight @00:25:32.06 Forearm.seek 3 + 700 counts @00:25:33.18 SE.reconfigure :pick @00:25:33.23 SE.seek :beamBreak @00:25:34.77 SE.configure :default @00:25:34.91 Elbow.seek 3 @00:25:35.08 Forearm.seek 3 + 300 counts @00:25:35.36 Forearm.reconfigure :loose @00:25:35.40 Hand.close @00:25:36.04 SE.seek :down @00:25:36.23
Forearm.seek :retract @00:25:37.23 Elbow.seek PC @00:25:37.29 SE.stop @00:25:39.85
Elbow.reconfigure :loose @00:25:39.94 Forearm.reconfigure :tight @00:25:39.98 Forearm.seek PC @00:25:41.18 PC.seek :closed @00:25:45.04 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.38 @00:25:45.05 Hand.open @00:25:45.84 Forearm.reconfigure :loose @00:25:45.88 Forearm.seek :retract @00:25:46.87 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @00:25:46.88 PH.seek 28C,5:00 @00:26:32.45 PH.hold 2:00:00,28C @00:26:32.49 delay 1:30 @00:28:02.52 PSR.select :air @00:28:02.53 PSV.dial PSR @00:28:03.58 Pulling 0.5ml of PSR.wash PSR.select :wash @00:28:03.70 PS.seek 0.5ml @00:28:09.54 delay 5 seconds @00:28:14.56 PSR.select :air @00:28:14.67 PS.seek 2ml @00:28:30.66 PTV.dial :puck @00:28:31.35 PSV.dial PTV @00:28:32.07 PBV.dial :waste1 @00:28:33.38 PS.seek 1.5ml @00:28:39.26 delay 3 seconds @00:28:42.27 PBV.dial between PSV and waste1 @00:28:42.66 PS.seek 0ml @00:28:58.69 delay 5:00 @00:33:58.71 recoverPSRreagent08 @00:33:58.72 PTV.dial :puck @00:33:58.73 PBV.dial :waste1 @00:33:59.11 delay 30 seconds @00:34:29.13 PBV.dial between PSV and waste1 @00:34:29.50 PSR.select :air @00:34:29.51 PSV.dial PSR @00:34:30.24 PS.reconfigure :fast @00:34:30.28 PS.seek 2.5ml @00:34:52.93 PSV.dial PTV @00:34:53.64 delay 5 seconds @00:34:58.65 PSV.dial PSR @00:34:59.37 delay 5 seconds @00:35:04.39 PSV.dial PTV @00:35:05.11 delay 5 seconds @00:35:10.12 PSV.dial :air @00:35:10.85 PS.seek 0ml @00:35:33.15 PTV.dial :PRVmixing,via: PRV @00:35:34.38 PSV.dial :mixloop @00:35:34.82 PRV.dial :ab1 @00:35:35.53 PS.reconfigure :slow @00:35:35.57 PS.seek 0.15ml @00:35:39.09 delay 2 seconds @00:35:41.11 CBV.dial PRV @00:35:41.13
PRV.dial CBV @00:35:41.69 PS.seek 0.34ml @00:35:45.99 delay 2 seconds @00:35:48.01 CBV.dial between PRV and PSV @00:35:48.32 PRV.dial :air @00:35:48.77 PS.seek 0.9ml @00:36:00.59 PRV.dial :waste2 @00:36:01.30 PS.seek 0.5ml @00:36:09.93 addPMLReagent08 @00:36:09.94 PSV.dial :air @00:36:10.39 PS.reconfigure :default @00:36:10.43 PS.seek 2ml @00:36:26.52 PSV.dial :PML @00:36:26.96 PTV.dial :mixing @00:36:27.68 PBV.dial :waste1 @00:36:28.08 PS.seek 1.85ml @00:36:30.43 PBV.dial between PSV and waste1 @00:36:30.80 PS.seek 0ml @00:36:50.46 delay 2 seconds @00:36:52.48 Beginning samplePassC @00:36:52.49 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:36:52.65 Elbow.reconfigure :tight @00:36:52.69 Elbow.seek FlushPuck::Garage @00:36:54.03 Elbow.reconfigure :loose @00:36:54.12 Forearm.seek :garage @00:36:55.26 Hand.close @00:36:55.99 Forearm.seek :retract @00:36:57.12 Elbow.reconfigure :tight @00:36:57.16 Elbow.seek CC @00:37:00.01 Elbow.reconfigure :loose @00:37:00.10 Forearm.reconfigure :tight @00:37:00.14 Forearm.seek CC @00:37:01.38 CC.seek :closed @00:37:05.62 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.80 @00:37:05.68 Hand.open @00:37:06.32 Forearm.reconfigure :loose @00:37:06.36 Forearm.seek :retract @00:37:07.43 PSV.dial PSR @00:37:08.45 Pulling 1.5ml of PSR.flush @00:37:08.46 PSR.select :flush @00:37:08.58 PS.seek 1.5ml @00:37:24.65 delay 5 seconds @00:37:29.67 PSV.dial :waste1 @00:37:30.11 PS.seek 0ml @00:37:46.11 PSV.dial PSR @00:37:46.68 PSR.select :air @00:37:46.79 PS.seek 2.5ml @00:38:12.93 PRV.dial CBV,avoiding: :ab2 @00:38:12.96
CBV.dial PRV @00:38:14.52 PSV.dial :mixloop @00:38:15.52 CTV.dial :air,avoiding: :intake @00:38:15.54 PTV.dial :PRVmixing @00:38:16.25 PS.seek 0ml @00:38:42.46 PSV.dial :air @00:38:42.92 PS.seek 2.5ml @00:39:09.11 PSV.dial :mixloop @00:39:09.56 PS.seek 0ml @00:39:35.77 PSV.dial :air @00:39:36.21 PS.seek 2.5ml @00:40:02.42 PSV.dial :mixloop @00:40:02.87 PS.seek 0ml @00:40:29.05 Garaging FlushPuck in Collection Clamp @00:40:29.12 Elbow.reconfigure :tight @00:40:29.16 Elbow.seek CC @00:40:29.27 Elbow.reconfigure :loose @00:40:29.36 Forearm.seek CC @00:40:30.31 Hand.close @00:40:30.88 CC.seek :open @00:40:35.20 Forearm.seek :retract @00:40:36.16 Elbow.reconfigure :tight @00:40:36.20 Elbow.seek :garage @00:40:39.07 Elbow.reconfigure :loose @00:40:39.16 Forearm.reconfigure :tight @00:40:39.20 Forearm.seek :garage @00:40:40.31 Hand.open @00:40:41.06 Forearm.reconfigure :loose @00:40:41.10 Forearm.seek :retract @00:40:42.20 delayUntil "00:44:52.47" @00:40:42.23 Whole Cell Archive sampling 1000ml @00:40:42.25 Loading wcr puck from tube 3 into Collection Clamp @00:40:42.47 SC.reconfigure :tight @00:40:42.51 SC.seek 3 @00:40:42.57 SC.configure :limp @00:40:42.77 Elbow.reconfigure :tight @00:40:42.81 Elbow.seek 3 @00:40:44.75 Forearm.reconfigure :tight @00:40:44.80 Forearm.seek 3 + 700 counts @00:40:45.89 SE.reconfigure :pick @00:40:45.94 SE.seek :beamBreak @00:40:47.57 SE.configure :default @00:40:47.71 Elbow.seek 3 @00:40:47.88 Forearm.seek 3 + 300 counts @00:40:48.05 Forearm.reconfigure :loose @00:40:48.09 Hand.close @00:40:48.76 SE.seek :down @00:40:48.96 Forearm.seek :retract @00:40:49.94 Elbow.seek CC @00:40:50.05 SE.stop @00:40:51.45 Elbow.reconfigure :loose @00:40:51.54 Forearm.reconfigure :tight @00:40:51.59 Forearm.seek CC @00:40:53.30 CC.seek :closed @00:40:57.53 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.74 @00:40:57.54 Hand.open @00:40:58.31 Forearm.reconfigure :loose @00:40:58.35 Forearm.seek :retract @00:40:59.35 collectSample 1000ml w/bubble point of 35psi @00:40:59.36 Priming 7ml @00:40:59.37 Intake.select :close @00:40:59.38 Exhaust.select :close @00:40:59.39 SS.hold @00:40:59.58 Calibrating pressure sensors @00:40:59.59 CTV.dial :air @00:40:59.61 SSV.dial :spit,avoiding: :exhaust @00:40:59.63 SS.reconfigure :fast @00:40:59.67 SS.seek 5ml @00:41:02.41 SSV.dial CBV @00:41:02.44 CBV.dial SSV @00:41:03.76 delay 3.8 seconds @00:41:07.68 SSV.dial :spit @00:41:08.98 SS.seek 0ml @00:41:11.72 SSV.dial CBV @00:41:12.99 CTV.dial :intake @00:41:13.72 SSV.dial CBV @00:41:13.74 CBV.dial SSV @00:41:13.76 Allowing 40s for 7.0ml of remaining 7.0ml @00:41:13.77 Intake.select :open @00:41:15.92 Intake @15.8psia, vacuum from 10.0 to 13.0psi @00:41:15.94 SS.reconfigure :inhaling 2.14ml/s @00:41:15.98 SS.seek 7ml @00:41:22.33 Stroke completed -- waiting for pressure to equalize ... @00:41:22.34 SS.reconfigure :exhale @00:41:22.38 SS.hold @00:41:32.03 Intake.select :close @00:41:32.18 SSV.dial :exhaust @00:41:32.91 Exhaust.select :open @00:41:33.03 SS.seek 0ml @00:41:37.20 Exhaust.select :close @00:41:37.31 Sampling 1000.0ml @00:41:37.32 Intake.select :close @00:41:37.33 Exhaust.select :close @00:41:37.34 SS.reconfigure :default @00:41:37.38 SS.hold @00:41:37.58 CTV.dial :intake @00:41:37.59 SSV.dial CBV @00:41:37.62 CBV.dial SSV @00:41:38.38 Allowing 130s for 25.0ml of remaining 1000.0ml @00:41:38.39 Intake.select :open @00:41:40.54 Intake @15.9psia, vacuum from 10.0 to 13.0psi @00:41:40.56 SS.reconfigure :inhaling 2.14ml/s @00:41:40.61 SS.seek 25ml @00:41:44.37 Vacuum too high -- waiting up to 40 seconds @00:41:44.38 SS.reconfigure :exhale @00:41:44.42 SS.hold @00:41:46.38 Intake @15.5psia, vacuum from 10.0 to 13.0psi @00:41:46.40 SS.reconfigure :inhaling 1.93ml/s @00:41:46.44 SS.seek 25ml @00:41:49.21 Vacuum too high -- waiting up to 40 seconds @00:41:49.23 SS.reconfigure :exhale @00:41:49.27 SS.hold @00:41:52.02 Intake @16.1psia, vacuum from 10.0 to 13.0psi @00:41:52.04 SS.reconfigure :inhaling 1.74ml/s @00:41:52.09 SS.seek 25ml @00:41:55.71 Vacuum too high -- waiting up to 40 seconds @00:41:55.72 SS.reconfigure :exhale @00:41:55.77 SS.hold @00:41:57.61 Intake @15.7psia, vacuum from 10.0 to 13.0psi @00:41:57.63 SS.reconfigure :inhaling 1.57ml/s @00:41:57.68 SS.seek 25ml @00:42:00.87 Vacuum too high -- waiting up to 40 seconds @00:42:00.88 SS.reconfigure :exhale @00:42:00.92 SS.hold @00:42:03.09 Intake @15.5psia, vacuum from 10.0 to 13.0psi @00:42:03.11 SS.reconfigure :inhaling 1.41ml/s @00:42:03.15 SS.seek 25ml @00:42:06.62 Vacuum too high -- waiting up to 40 seconds @00:42:06.64 SS.reconfigure :exhale @00:42:06.68 SS.hold @00:42:08.81 Intake @15.5psia, vacuum from 10.0 to 13.0psi @00:42:08.83 SS.reconfigure :inhaling 1.27ml/s @00:42:08.88 SS.seek 25ml @00:42:13.59 Vacuum too high -- waiting up to 40 seconds @00:42:13.60 SS.reconfigure :exhale @00:42:13.64 SS.hold @00:42:15.77 Intake @15.3psia, vacuum from 9.8 to 12.8psi @00:42:15.79 SS.reconfigure :inhaling 1.14ml/s @00:42:15.83 SS.seek 25ml @00:42:16.33 Stroke completed -- waiting for pressure to equalize ... @00:42:16.34 SS.reconfigure :exhale @00:42:16.38 SS.hold @00:42:17.56 Intake.select :close @00:42:17.70 SSV.dial :exhaust @00:42:18.44 Exhaust.select :open @00:42:18.55 SS.seek 0ml @00:42:27.62 Exhaust.select :close @00:42:27.73 SSV.dial CBV @00:42:27.76 CBV.dial SSV @00:42:28.50 Allowing 130s for 25.0ml of remaining 975.0ml @00:42:28.52 Intake.select :open @00:42:30.67 Intake @15.7psia, vacuum from 10.0 to 13.0psi @00:42:30.69 SS.reconfigure :inhaling 1.14ml/s @00:42:30.73 SS.seek 25ml @00:42:34.43 Vacuum too high -- waiting up to 40 seconds @00:42:34.44 SS.reconfigure :exhale @00:42:34.48 SS.hold @00:42:35.43 Intake @15.1psia, vacuum from 9.6 to 12.6psi @00:42:35.45 SS.reconfigure :inhaling 1.03ml/s @00:42:35.49 SS.seek 25ml @00:42:40.55 Vacuum too high -- waiting up to 40 seconds @00:42:40.57 SS.reconfigure :exhale @00:42:40.61 SS.hold @00:42:41.89 SS.reconfigure :inhaling 0.93ml/s @00:42:41.93 SS.seek 25ml @00:42:49.65 Vacuum too high -- waiting up to 40 seconds @00:42:49.66 SS.reconfigure :exhale @00:42:49.70 SS.hold @00:42:51.34 Intake @15.3psia, vacuum from 9.8 to 12.8psi @00:42:51.36 SS.reconfigure :inhaling 0.84ml/s @00:42:51.40 SS.seek 25ml @00:43:01.37 Vacuum too high -- waiting up to 40 seconds @00:43:01.38 SS.reconfigure :exhale @00:43:01.42 SS.hold @00:43:03.37 Intake @15.3psia, vacuum from 9.8 to 12.8psi @00:43:03.38 SS.reconfigure :inhaling 0.76ml/s @00:43:03.43 SS.seek 25ml @00:43:08.19 Stroke completed -- waiting for pressure to equalize ... @00:43:08.20 SS.reconfigure :exhale @00:43:08.24 SS.hold @00:43:10.19 Intake.select :close @00:43:10.34 SSV.dial :exhaust @00:43:11.08 Exhaust.select :open @00:43:11.19 SS.seek 0ml @00:43:20.62 Exhaust.select :close @00:43:20.73 SSV.dial CBV @00:43:20.77 CBV.dial SSV @00:43:21.50 Allowing 130s for 25.0ml of remaining 950.0ml @00:43:21.52 Intake.select :open @00:43:23.67 Intake @15.6psia, vacuum from 10.0 to 13.0psi @00:43:23.69 SS.reconfigure :inhaling 0.76ml/s @00:43:23.73 SS.seek 25ml @00:43:41.99 Vacuum too high -- waiting up to 40 seconds @00:43:42.00 SS.reconfigure :exhale @00:43:42.05 SS.hold @00:43:43.72 Intake @15.3psia, vacuum from 9.8 to 12.8psi @00:43:43.74 SS.reconfigure :inhaling 0.69ml/s @00:43:43.78 SS.seek 25ml @00:44:02.26 Stroke completed -- waiting for pressure to equalize ... @00:44:02.27 SS.reconfigure :exhale @00:44:02.32 SS.hold @00:44:07.07 Intake.select :close @00:44:07.21 SSV.dial :exhaust @00:44:07.94 Exhaust.select :open @00:44:08.09 SS.seek 0ml @00:44:17.39 Exhaust.select :close @00:44:17.49 SSV.dial CBV @00:44:17.52 CBV.dial SSV @00:44:18.26 Allowing 130s for 25.0ml of remaining 925.0ml @00:44:18.28 Intake.select :open @00:44:20.43 Intake @15.6psia, vacuum from 10.0 to 13.0psi @00:44:20.45 SS.reconfigure :inhaling 0.69ml/s @00:44:20.49 SS.seek 25ml @00:44:31.93 Vacuum too high -- waiting up to 40 seconds @00:44:31.94 SS.reconfigure :exhale @00:44:31.98 SS.hold @00:44:33.62 Intake @15.4psia, vacuum from 9.9 to 12.9psi @00:44:33.64 SS.reconfigure :inhaling 0.61ml/s @00:44:33.68 SS.seek 25ml @00:44:52.48 recoverPRVreagent08 @00:44:52.49 PTV.dial :mixing @00:44:53.20 PBV.dial :waste1 @00:44:53.56 delay 30 seconds @00:44:54.11 Vacuum too high -- waiting up to 40 seconds @00:44:54.12 SS.reconfigure :exhale @00:44:54.17 SS.hold @00:44:57.34 Intake @15.5psia, vacuum from 10.0 to 13.0psi @00:44:57.36 SS.reconfigure :inhaling 0.56ml/s @00:44:57.40 SS.seek 25ml @00:45:08.49 Stroke completed -- waiting for pressure to equalize ... @00:45:08.51 SS.reconfigure :exhale @00:45:08.55 SS.hold @00:45:13.89 Intake.select :close @00:45:14.04 SSV.dial :exhaust @00:45:14.76 Exhaust.select :open @00:45:14.87 SS.seek 0ml @00:45:23.58 PBV.dial between PSV and waste1 @00:45:23.93 PSV.dial :air @00:45:24.21 Exhaust.select :close @00:45:24.32 SSV.dial CBV @00:45:24.35 CBV.dial SSV @00:45:24.42 PS.reconfigure :fast @00:45:24.47 PS.seek 2.5ml @00:45:25.12 Allowing 130s for 25.0ml of remaining 900.0ml @00:45:25.14 Intake.select :open @00:45:27.30 Intake @15.5psia, vacuum from 10.0 to 13.0psi @00:45:27.31 SS.reconfigure :inhaling 0.56ml/s @00:45:27.36 SS.seek 25ml @00:45:42.33 Vacuum too high -- waiting up to 40 seconds @00:45:42.34 SS.reconfigure :exhale @00:45:42.38 SS.hold @00:45:44.77 Intake @15.3psia, vacuum from 9.8 to 12.8psi @00:45:44.79 SS.reconfigure :inhaling 0.50ml/s @00:45:44.83 SS.seek 25ml @00:45:47.13 PSV.dial :mixloop @00:45:47.59 delay 2 seconds @00:45:49.60 PSV.dial :air @00:45:50.06 delay 2 seconds @00:45:52.07 PSV.dial :mixloop @00:45:52.53 delay 2 seconds @00:45:54.54 PSV.dial :air @00:45:55.00 PS.seek 0ml @00:46:05.83 Vacuum too high -- waiting up to 40 seconds @00:46:05.84 SS.reconfigure :exhale @00:46:05.88 SS.hold @00:46:10.05 Intake @15.4psia, vacuum from 9.9 to 12.9psi @00:46:10.07 SS.reconfigure :inhaling 0.46ml/s @00:46:10.11 SS.seek 25ml @00:46:17.54 washCycleDA #1 of 2 @00:46:17.55 PSR.select :air @00:46:17.57 PSV.dial PSR @00:46:18.87 Pulling 0.5ml of PSR.wash @00:46:18.88 PSR.select :wash @00:46:18.99 PS.reconfigure :default @00:46:19.03 PS.seek 0.5ml @00:46:25.13 delay 5 seconds @00:46:27.16 Stroke completed -- waiting for pressure to equalize ... @00:46:27.18 SS.reconfigure :exhale @00:46:27.22 SS.hold @00:46:30.18 PSR.select :air @00:46:30.28 PS.seek 2ml @00:46:34.68 Intake.select :close @00:46:34.82 SSV.dial :exhaust @00:46:35.57 Exhaust.select :open @00:46:35.68 SS.seek 0ml @00:46:44.98 Exhaust.select :close @00:46:45.08 SSV.dial CBV @00:46:45.11 CBV.dial SSV @00:46:45.87 Allowing 130s for 25.0ml of remaining 875.0ml @00:46:45.88 Intake.select :open @00:46:46.29 PTV.dial :puck @00:46:46.98 PSV.dial PTV @00:46:47.69 PBV.dial :waste1 @00:46:48.04 Intake @15.5psia, vacuum from 10.0 to 13.0psi @00:46:48.06 SS.reconfigure :inhaling 0.46ml/s @00:46:48.15 SS.seek 25ml @00:46:48.22 PS.seek 1.5ml @00:46:54.19 delay 3 seconds @00:46:57.21 PBV.dial between PSV and waste1 @00:46:57.58 PS.seek 0ml @00:47:06.45 Vacuum too high -- waiting up to 40 seconds @00:47:06.46 SS.reconfigure :exhale @00:47:06.51 SS.hold @00:47:09.37 Intake @15.3psia, vacuum from 9.8 to 12.8psi @00:47:09.39 SS.reconfigure :inhaling 0.41ml/s @00:47:09.43 SS.seek 25ml @00:47:13.54 delay 2:00 @00:47:31.95 Vacuum too high -- waiting up to 40 seconds @00:47:31.96 SS.reconfigure :exhale @00:47:32.01 SS.hold @00:47:37.67 Intake @15.9psia, vacuum from 10.0 to 13.0psi @00:47:37.69 SS.reconfigure :inhaling 0.37ml/s @00:47:37.73 SS.seek 25ml @00:48:05.35 Stroke completed -- waiting for pressure to equalize ... @00:48:05.36 SS.reconfigure :exhale @00:48:05.40 SS.hold @00:48:16.41 Intake.select :close @00:48:16.55 SSV.dial :exhaust @00:48:17.29 Exhaust.select :open @00:48:17.40 SS.seek 0ml @00:48:26.41 Exhaust.select :close @00:48:26.52 SSV.dial CBV @00:48:26.54 CBV.dial SSV @00:48:27.29 Allowing 130s for 25.0ml of remaining 850.0ml @00:48:27.30 Intake.select :open @00:48:29.45 Intake @15.6psia, vacuum from 10.0 to 13.0psi @00:48:29.47 SS.reconfigure :inhaling 0.37ml/s @00:48:29.52 SS.seek 25ml @00:49:05.35 Vacuum too high -- waiting up to 40 seconds @00:49:05.37 SS.reconfigure :exhale @00:49:05.41 SS.hold @00:49:11.78 Intake @15.6psia, vacuum from 10.0 to 13.0psi @00:49:11.80 SS.reconfigure :inhaling 0.33ml/s @00:49:11.84 SS.seek 25ml @00:49:13.56 recoverPSRreagent08 @00:49:13.57 PTV.dial :puck @00:49:13.58 PBV.dial :waste1 @00:49:13.93 delay 30 seconds @00:49:43.95 PBV.dial between PSV and waste1 @00:49:44.30 PSR.select :air @00:49:44.32 PSV.dial PSR @00:49:45.04 PS.reconfigure :fast @00:49:45.08 PS.seek 2.5ml @00:49:49.79 Vacuum too high -- waiting up to 40 seconds @00:49:49.81 SS.reconfigure :exhale @00:49:49.85 SS.hold @00:50:00.11 Intake @15.8psia, vacuum from 10.0 to 13.0psi @00:50:00.12 SS.reconfigure :inhaling 0.30ml/s @00:50:00.17 SS.seek 25ml @00:50:07.69 PSV.dial PTV @00:50:08.41 delay 5 seconds @00:50:13.16 Stroke completed -- waiting for pressure to equalize ... @00:50:13.17 SS.reconfigure :exhale @00:50:13.21 SS.hold @00:50:13.42 PSV.dial PSR @00:50:14.15 delay 5 seconds @00:50:19.17 PSV.dial PTV @00:50:19.88 delay 5 seconds @00:50:24.89 PSV.dial :air @00:50:25.62 PS.seek 0ml @00:50:25.89 Intake.select :close @00:50:26.04 SSV.dial :exhaust @00:50:26.77 Exhaust.select :open @00:50:26.88 SS.seek 0ml @00:50:36.03 Exhaust.select :close @00:50:36.14 SSV.dial CBV @00:50:36.17 CBV.dial SSV @00:50:36.91 Allowing 130s for 25.0ml of remaining 825.0ml @00:50:36.92 Intake.select :open @00:50:39.08 Intake @15.6psia, vacuum from 10.0 to 13.0psi @00:50:39.09 SS.reconfigure :inhaling 0.30ml/s @00:50:39.14 SS.seek 25ml @00:50:48.25 washCycleDA #2 of 2 @00:50:48.26 PSR.select :air @00:50:48.28 PSV.dial PSR @00:50:49.59 Pulling 0.5ml of PSR.wash @00:50:49.60 PSR.select :wash @00:50:49.71 PS.reconfigure :default @00:50:49.75 PS.seek 0.5ml @00:50:55.72 delay 5 seconds @00:51:00.74 PSR.select :air @00:51:00.85 PS.seek 2ml @00:51:13.19 Vacuum too high -- waiting up to 40 seconds @00:51:13.21 SS.reconfigure :exhale @00:51:13.25 SS.hold @00:51:16.84 PTV.dial :puck @00:51:16.85 PSV.dial PTV @00:51:17.54 PBV.dial :waste1 @00:51:18.02 PS.seek 1.5ml @00:51:19.63 Intake @15.7psia, vacuum from 10.0 to 13.0psi @00:51:19.65 SS.reconfigure :inhaling 0.27ml/s @00:51:19.69 SS.seek 25ml @00:51:23.91 delay 3 seconds @00:51:26.92 PBV.dial between PSV and waste1 @00:51:27.29 PS.seek 0ml @00:51:43.38 delay 2:00 @00:51:56.25 Vacuum too high -- waiting up to 40 seconds @00:51:56.27 SS.reconfigure :exhale @00:51:56.31 SS.hold @00:52:04.74 Intake @15.8psia, vacuum from 10.0 to 13.0psi @00:52:04.76 SS.reconfigure :inhaling 0.24ml/s @00:52:04.80 SS.seek 25ml @00:52:46.18 Stroke completed -- waiting for pressure to equalize ... @00:52:46.19 SS.reconfigure :exhale @00:52:46.24 SS.hold @00:52:46.93 SS.reconfigure :default @00:52:47.47 SS.hold @00:52:47.58 Intake.select :close @00:52:47.73 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 200.0ml @00:52:47.74 SSV.dial :exhaust @00:52:48.48 Exhaust.select :open @00:52:48.59 SS.reconfigure :exhale @00:52:48.64 SS.seek 0ml @00:52:57.84 Exhaust.select :close @00:52:57.95 Sampled 200.0ml @00:52:57.96 Drying puck before fixing for 20:00 @00:52:57.97 CBV.dial :waste2b @00:52:58.68 delay 1:00 @00:53:43.39 recoverPSRreagent08 @00:53:43.40 PTV.dial :puck @00:53:43.41 PBV.dial :waste1 @00:53:43.76 delay 30 seconds @00:53:58.70 CSV.dial :air @00:53:59.15 CS.reconfigure :fast @00:53:59.20 CS.seek 10ml @00:54:13.78 PBV.dial between PSV and waste1 @00:54:14.14 PSR.select :air @00:54:14.15 PSV.dial PSR @00:54:14.88 PS.reconfigure :fast @00:54:14.92 PS.seek 2.5ml @00:54:21.76 Pumping wcr puck from tube 3 up to 35psi... @00:54:21.77 CBV.dial SSV @00:54:23.06 CBV.dial between CSV and waste2b @00:54:23.96 CSV.dial between air and CTV @00:54:24.28 CS.seek 7.321ml @00:54:31.07 CTV.dial CSV,avoiding: :air @00:54:31.10 CSV.dial CTV @00:54:31.87 CS.reconfigure :pressurize @00:54:31.92 CS.seek 0ml @00:54:37.59 PSV.dial PTV @00:54:38.31 delay 5 seconds @00:54:43.33 PSV.dial PSR @00:54:44.04 delay 5 seconds @00:54:45.49 CBV.dial :waste2b @00:54:45.82 CS.reconfigure :default @00:54:45.87 CS.seek 0ml @00:54:49.06 PSV.dial PTV @00:54:49.78 delay 5 seconds @00:54:51.88 delay 1:00 @00:54:54.79 PSV.dial :air @00:54:55.51 PS.seek 0ml @00:55:17.93 getPMLReagent08 @00:55:17.94 PRV.dial :air @00:55:18.37 delay 5 seconds @00:55:23.39 PTV.dial :PRVmixing @00:55:24.62 delay 5 seconds @00:55:29.64 PRV.dial :ab2 @00:55:30.07 PTV.dial :PRVmixing @00:55:30.08 PSV.dial :mixloop @00:55:30.53 PS.reconfigure :default @00:55:30.57 PS.seek 0.3ml @00:55:34.52 delay 2 seconds @00:55:36.54 PRV.dial :air @00:55:37.04 PS.seek 0.55ml @00:55:40.44 addPMLReagent08 @00:55:40.45 PSV.dial :air @00:55:40.90 PS.seek 2ml @00:55:51.91 CTV.dial :air,avoiding: :intake @00:55:53.20 Fix #1 of 2 (with RNAlater) for 10:00 @00:55:53.21 CSV.dial CSR @00:55:53.68 Pulling 2ml of CSR.RNAlater @00:55:53.69 CSR.select :RNAlater @00:55:53.80 CS.seek 2ml @00:55:56.40 PSV.dial :PML @00:55:56.83 PTV.dial :mixing @00:55:57.56 PBV.dial :waste1 @00:55:57.98 PS.seek 1.85ml @00:55:59.69 delay 5 seconds @00:56:00.30 PBV.dial between PSV and waste1 @00:56:00.67 PS.seek 0ml @00:56:04.71 CSR.select :air @00:56:04.82 CS.reconfigure :fast @00:56:04.86 CS.seek 10ml @00:56:20.27 delay 2 seconds @00:56:22.29 delay 8:00 @00:56:23.13 Pumping wcr puck from tube 3 up to 35psi... @00:56:23.14 CBV.dial SSV @00:56:24.41 CBV.dial between CSV and waste2b @00:56:25.33 CSV.dial between air and CTV @00:56:25.92 CS.seek 7.365ml @00:56:32.94 CTV.dial CSV,avoiding: :air @00:56:32.97 CSV.dial CTV @00:56:34.32 CS.reconfigure :pressurize @00:56:34.37 CS.seek 0ml @00:56:51.59 delay 10:00 @01:04:22.31 recoverPRVreagent08 @01:04:22.32 PTV.dial :mixing @01:04:22.34 PBV.dial :waste1 @01:04:22.68 delay 30 seconds @01:04:52.71 PBV.dial between PSV and waste1 @01:04:53.06 PSV.dial :air @01:04:53.51 PS.reconfigure :fast @01:04:53.56 PS.seek 2.5ml @01:05:16.16 PSV.dial :mixloop @01:05:16.60 delay 2 seconds @01:05:18.62 PSV.dial :air @01:05:19.07 delay 2 seconds @01:05:21.09 PSV.dial :mixloop @01:05:21.55 delay 2 seconds @01:05:23.56 PSV.dial :air @01:05:24.01 PS.seek 0ml @01:05:46.44 washCycleDA #1 of 3 @01:05:46.45 PSR.select :air @01:05:46.47 PSV.dial PSR @01:05:47.78 Pulling 0.5ml of PSR.wash @01:05:47.79 PSR.select :wash @01:05:47.90 PS.reconfigure :default @01:05:47.94 PS.seek 0.5ml @01:05:53.91 delay 5 seconds @01:05:58.93 PSR.select :air @01:05:59.04 PS.seek 2ml @01:06:15.10 PTV.dial :puck @01:06:15.79 PSV.dial PTV @01:06:16.51 PBV.dial :waste1 @01:06:16.92 PS.seek 1.5ml @01:06:22.75 delay 3 seconds @01:06:25.77 PBV.dial between PSV and waste1 @01:06:26.14 PS.seek 0ml @01:06:42.19 delay 2:00 @01:06:51.61 CBV.dial :waste2b @01:06:51.93 CS.reconfigure :default @01:06:51.97 CS.seek 0ml @01:06:54.45 delay 1:00 @01:07:54.48 CTV.dial :air,avoiding: :intake @01:07:55.75 Fix #2 of 2 (with RNAlater) for 10:00 @01:07:55.77 CSV.dial CSR @01:07:56.24 Pulling 2ml of CSR.RNAlater @01:07:56.26 CSR.select :RNAlater @01:07:56.37 CS.seek 2ml @01:08:02.17 delay 5 seconds @01:08:07.19 CSR.select :air @01:08:07.30 CS.reconfigure :fast @01:08:07.35 CS.seek 10ml @01:08:25.58 Pumping wcr puck from tube 3 up to 35psi... @01:08:25.59 CBV.dial SSV @01:08:26.84 CBV.dial between CSV and waste2b @01:08:27.76 CSV.dial between air and CTV @01:08:28.35 CS.seek 7.291ml @01:08:35.08 CTV.dial CSV,avoiding: :air @01:08:35.11 CSV.dial CTV @01:08:36.44 CS.reconfigure :pressurize @01:08:36.49 CS.seek 0ml @01:08:42.21 recoverPSRreagent08 @01:08:42.22 PTV.dial :puck @01:08:42.24 PBV.dial :waste1 @01:08:42.58 delay 30 seconds @01:08:53.48 delay 10:00 @01:09:12.60 PBV.dial between PSV and waste1 @01:09:12.95 PSR.select :air @01:09:12.97 PSV.dial PSR @01:09:13.69 PS.reconfigure :fast @01:09:13.74 PS.seek 2.5ml @01:09:36.34 PSV.dial PTV @01:09:37.05 delay 5 seconds @01:09:42.07 PSV.dial PSR @01:09:42.78 delay 5 seconds @01:09:47.80 PSV.dial PTV @01:09:48.52 delay 5 seconds @01:09:53.53 PSV.dial :air @01:09:54.25 PS.seek 0ml @01:10:16.53 washCycleDA #2 of 3 @01:10:16.54 PSR.select :air @01:10:16.56 PSV.dial PSR @01:10:17.97 Pulling 0.5ml of PSR.wash @01:10:17.98 PSR.select :wash @01:10:18.09 PS.reconfigure :default @01:10:18.13 PS.seek 0.5ml @01:10:24.00 delay 5 seconds @01:10:29.02 PSR.select :air @01:10:29.13 PS.seek 2ml @01:10:45.25 PTV.dial :puck @01:10:45.26 PSV.dial PTV @01:10:45.98 PBV.dial :waste1 @01:10:46.87 PS.seek 1.5ml @01:10:52.75 delay 3 seconds @01:10:55.77 PBV.dial between PSV and waste1 @01:10:56.13 PS.seek 0ml @01:11:12.19 delay 2:00 @01:13:12.21 recoverPSRreagent08 @01:13:12.22 PTV.dial :puck @01:13:12.23 PBV.dial :waste1 @01:13:12.59 delay 30 seconds @01:13:42.61 PBV.dial between PSV and waste1 @01:13:42.96 PSR.select :air @01:13:42.98 PSV.dial PSR @01:13:43.70 PS.reconfigure :fast @01:13:43.75 PS.seek 2.5ml @01:14:06.24 PSV.dial PTV @01:14:06.96 delay 5 seconds @01:14:11.98 PSV.dial PSR @01:14:12.70 delay 5 seconds @01:14:17.72 PSV.dial PTV @01:14:18.43 delay 5 seconds @01:14:23.45 PSV.dial :air @01:14:24.17 PS.seek 0ml @01:14:46.55 washCycleDA #3 of 3 @01:14:46.56 PSR.select :air @01:14:46.58 PSV.dial PSR @01:14:47.87 Pulling 0.5ml of PSR.wash @01:14:47.88 PSR.select :wash @01:14:48.00 PS.reconfigure :default @01:14:48.04 PS.seek 0.5ml @01:14:54.09 delay 5 seconds @01:14:59.11 PSR.select :air @01:14:59.21 PS.seek 2ml @01:15:15.24 PTV.dial :puck @01:15:15.25 PSV.dial PTV @01:15:16.07 PBV.dial :waste1 @01:15:16.47 PS.seek 1.5ml @01:15:22.36 delay 3 seconds @01:15:25.38 PBV.dial between PSV and waste1 @01:15:25.75 PS.seek 0ml @01:15:41.80 delay 2:00 @01:17:41.83 recoverPSRreagent08 @01:17:41.84 PTV.dial :puck @01:17:41.85 PBV.dial :waste1 @01:17:42.21 delay 30 seconds @01:18:12.23 PBV.dial between PSV and waste1 @01:18:12.58 PSR.select :air @01:18:12.60 PSV.dial PSR @01:18:13.30 PS.reconfigure :fast @01:18:13.35 PS.seek 2.5ml @01:18:35.95 PSV.dial PTV @01:18:36.67 delay 5 seconds @01:18:41.69 PSV.dial PSR @01:18:42.41 delay 5 seconds @01:18:47.43 PSV.dial PTV @01:18:48.14 delay 5 seconds @01:18:53.16 PSV.dial :air @01:18:53.49 CBV.dial :waste2b @01:18:53.80 CS.reconfigure :default @01:18:53.85 CS.seek 0ml @01:18:53.91 PS.seek 0ml @01:18:56.44 delay 1:00 @01:19:16.45 Gate.power :camera,:ON @01:19:16.47 Mixing DA Substrates PSR.select :air @01:19:16.49 PSV.dial PSR @01:19:18.02 Pulling 0.25ml of PSR.sub1 @01:19:18.05 PSR.select :sub1 @01:19:18.35 PS.reconfigure :default @01:19:18.51 PS.seek 0.25ml @01:19:21.99 delay 5 seconds @01:19:27.05 Pulling 0.25ml of PSR.sub2 @01:19:27.06 PSR.select :sub2 @01:19:27.18 PS.seek 0.5ml @01:19:30.58 delay 5 seconds @01:19:35.60 PSR.select :air @01:19:35.70 PS.seek 2ml @01:19:51.76 PTV.dial :puck @01:19:51.77 PSV.dial PTV @01:19:52.48 PBV.dial :waste1 @01:19:52.90 PS.seek 1.5ml @01:19:56.45 CTV.dial :air,avoiding: :intake @01:19:58.82 delay 3 seconds @01:20:01.84 PBV.dial between PSV and waste1 @01:20:02.21 PS.seek 0ml @01:20:18.39 delay 10 seconds @01:20:28.41 recoverPSRreagent08 @01:20:28.42 PTV.dial :puck @01:20:28.43 PBV.dial :waste1 @01:20:28.78 delay 30 seconds @01:20:58.80 PBV.dial between PSV and waste1 @01:20:59.15 PSR.select :air @01:20:59.17 PSV.dial PSR @01:21:00.02 PS.reconfigure :fast @01:21:00.06 PS.seek 2.5ml @01:21:22.79 PSV.dial PTV @01:21:23.50 delay 5 seconds @01:21:28.51 PSV.dial PSR @01:21:29.23 delay 5 seconds @01:21:34.25 PSV.dial PTV @01:21:34.96 delay 5 seconds @01:21:39.98 PSV.dial :air @01:21:40.70 PS.seek 0ml @01:22:03.17 Additional air clear @01:22:03.18 PSR.select :air @01:22:03.19 PSV.dial PSR @01:22:04.50 Pulling 2ml of PSR.air @01:22:04.51 PSR.select :air @01:22:04.53 PS.reconfigure :default @01:22:04.57 PS.seek 2ml @01:22:25.82 delay 5 seconds @01:22:30.84 PTV.dial :puck @01:22:30.86 PSV.dial PTV @01:22:31.57 PBV.dial :waste1 @01:22:32.49 PS.seek 1.5ml @01:22:38.38 delay 3 seconds @01:22:41.40 PBV.dial between PSV and waste1 @01:22:41.76 delay 1 second @01:22:42.78 PSV.dial :waste1 @01:22:43.78 PS.seek 0ml @01:22:59.90 Processing Puck Heater at 28.1C before imaging @01:22:59.91 PH.coast @01:23:00.03 Imaging da puck in Processing Clamp while storing it to tube 2 @01:23:00.19 SC.reconfigure :tight @01:23:00.23 SC.seek 2 @01:23:03.82 SC.configure :limp @01:23:04.01 Elbow.reconfigure :tight @01:23:04.05 Elbow.seek 2 @01:23:05.75 Forearm.reconfigure :tight @01:23:05.80 Forearm.seek 2 + 700 counts @01:23:06.83 SE.reconfigure :pick @01:23:06.88 SE.seek :beamBreak @01:23:09.44 SE.configure :default @01:23:09.58 Elbow.reconfigure :limp @01:23:09.63 Forearm.reconfigure :loose @01:23:09.67 Forearm.seek 2 @01:23:10.05 Elbow.hold @01:23:10.11 Elbow.reconfigure :tight @01:23:10.20 SE.seek 8.23 Pucks below Top Plate @01:23:10.66 Forearm.force -20 @01:23:10.69 Forearm.seek 2 + 316 counts @01:23:11.02 Forearm.reconfigure :tight @01:23:11.07 Forearm.seek 2 + 700 counts @01:23:11.40 SE.seek 9.23 Pucks below Top Plate @01:23:12.00 Forearm.reconfigure :loose @01:23:12.04 Forearm.seek :retract @01:23:12.93 Elbow.seek PC @01:23:15.42 Elbow.reconfigure :loose @01:23:15.51 Forearm.seek PC @01:23:16.58 Hand.close @01:23:17.29 PC.seek :open @01:23:21.29 Forearm.seek :retract @01:23:22.32 Elbow.reconfigure :tight @01:23:22.36 Elbow.seek 2 @01:23:25.11 Forearm.reconfigure :tight @01:23:25.16 Forearm.seek 2 + 700 counts @01:23:26.05 Hand.open @01:23:26.85 SE.seek 8.88 Pucks below Top Plate @01:23:27.13 SE.reconfigure :seal @01:23:27.18 SE.seek 7.77 Pucks below Top Plate @01:23:27.76 Lights, camera, ACTION! @01:23:27.80 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22dec0901h350ml.tif @01:23:45.20 SX MX5/16 (USB:19): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 13.907 seconds /var/log/esp/da22dec0901h350ml.tif: TIFF Upload Complete @01:23:45.23 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22dec0901h350ml15s.tif @01:23:47.23 Waiting >20 seconds for da to relinquish FlushPuck @01:24:00.97 SX MX5/16 (USB:19): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22dec0901h350ml15s.tif: TIFF Upload Complete @01:24:00.99 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da22dec0901h350ml25s.tif @01:24:26.73 SX MX5/16 (USB:19): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da22dec0901h350ml25s.tif: TIFF Upload Complete @01:24:26.74 Gate.power :camera,:OFF @01:24:26.76 DAfans.email "13.907 sec auto-exposure",Subject: "da22dec0901h350ml images!" @01:24:27.07 Elbow.seek 2 @01:24:27.46 Forearm.seek 2 + 700 counts @01:24:27.53 SE.reconfigure :gently @01:24:27.60 SE.seek 8.49 Pucks below Top Plate @01:24:28.31 Elbow.reconfigure :limp @01:24:28.36 Forearm.reconfigure :loose @01:24:28.40 Forearm.seek 2 @01:24:28.82 Elbow.hold @01:24:28.90 Elbow.reconfigure :tight @01:24:28.94 SE.reconfigure :default @01:24:28.99 SE.seek 8.67 Pucks below Top Plate @01:24:29.34 Forearm.force -20 @01:24:29.97 Forearm.seek 2 + 314 counts @01:24:30.30 Forearm.reconfigure :tight @01:24:30.35 Forearm.seek 2 + 700 counts @01:24:30.72 SE.seek :down @01:24:31.85 Forearm.reconfigure :loose @01:24:31.89 Forearm.seek :retract @01:24:32.88 SE.stop @01:24:33.01 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @01:24:33.34 Elbow.seek FlushPuck::Garage @01:24:35.22 Elbow.reconfigure :loose @01:24:35.41 Forearm.seek :garage @01:24:36.54 Hand.close @01:24:37.47 Forearm.seek :retract @01:24:38.57 Elbow.reconfigure :tight @01:24:38.69 Elbow.seek PC @01:24:40.15 Elbow.reconfigure :loose @01:24:40.37 Forearm.reconfigure :tight @01:24:40.47 Forearm.seek PC @01:24:42.15 PC.seek :closed @01:24:46.30 Processing Clamp onSpring/offSpring for 9/24 samples, on/off torque 1.39 @01:24:46.46 Hand.open @01:24:47.30 Forearm.reconfigure :loose @01:24:47.38 Forearm.seek :retract @01:24:48.52 PSR.select :air @01:24:48.58 Storing wcr puck in Collection Clamp to tube 2 @01:24:48.62 PSV.dial PSR @01:24:49.46 Pulling 0.5ml of PSR.flush @01:24:49.50 PSR.select :flush @01:24:49.88 Elbow.reconfigure :tight @01:24:49.96 Elbow.seek 2 @01:24:50.08 PS.seek 0.5ml @01:24:52.94 Forearm.reconfigure :tight @01:24:53.02 Forearm.seek 2 + 700 counts @01:24:54.22 SE.reconfigure :pick @01:24:54.36 SE.seek :beamBreak @01:24:56.07 delay 5 seconds @01:24:56.95 SE.configure :default @01:24:57.25 Elbow.reconfigure :limp @01:24:57.41 Forearm.reconfigure :loose @01:24:57.49 Forearm.seek 2 @01:24:57.93 Elbow.hold @01:24:58.05 Elbow.reconfigure :tight @01:24:58.25 SE.seek 9.24 Pucks below Top Plate @01:24:59.01 Forearm.force -20 @01:24:59.09 Forearm.seek 2 + 317 counts @01:24:59.72 Forearm.reconfigure :tight @01:24:59.82 Forearm.seek 2 + 700 counts @01:25:00.11 SE.seek 10.24 Pucks below Top Plate @01:25:00.85 Forearm.reconfigure :loose @01:25:00.93 Forearm.seek :retract @01:25:01.09 PSR.select :air @01:25:01.28 PS.seek 2.5ml @01:25:01.93 Elbow.seek CC @01:25:03.60 Elbow.reconfigure :loose @01:25:04.91 Forearm.seek CC @01:25:06.02 Hand.close @01:25:06.68 CC.seek :open @01:25:11.40 Forearm.seek :retract @01:25:12.43 Elbow.reconfigure :tight @01:25:12.53 Elbow.seek 2 @01:25:14.45 Elbow.reconfigure :limp @01:25:14.55 Forearm.seek 2 @01:25:15.68 Elbow.hold @01:25:15.87 Elbow.reconfigure :tight @01:25:15.97 Hand.open @01:25:17.05 Forearm.force -20 @01:25:17.16 Forearm.seek 2 + 319 counts @01:25:17.70 Forearm.reconfigure :tight @01:25:17.78 Forearm.seek 2 + 700 counts @01:25:18.16 SE.seek :down @01:25:19.42 Forearm.reconfigure :loose @01:25:19.52 Forearm.seek :retract @01:25:20.24 SE.stop @01:25:20.57
Starting finalPurge @01:25:22.46 delay 1 second @01:25:23.50 PRV.dial :waste2,avoiding: :ab2 @01:25:25.35 PTV.dial :PRVmixing @01:25:26.68 PSV.dial :mixloop @01:25:27.71 PS.seek :empty @01:25:40.33
Waiting >20 seconds for psFlush to relinquish FlushPuck @01:25:53.91 PSR.select :air @01:25:53.94 PSV.dial PSR @01:25:55.20 Pulling 0.5ml of PSR.flush @01:25:55.22 PSR.select :flush @01:25:55.39 PS.seek 0.5ml @01:26:01.58 delay 5 seconds @01:26:06.62 PSR.select :air @01:26:06.77 PS.seek 2.5ml @01:26:27.96 delay 1 second @01:26:29.00 PBV.dial :waste1 @01:26:29.38 PTV.dial :mixing @01:26:30.13 PSV.dial :mixloop @01:26:31.13 PS.seek :empty @01:26:57.41 PSV.dial PSR @01:26:58.44 PSR.select :flush @01:26:58.60 PS.seek 1ml @01:27:07.36 upsync @01:27:09.65 PSR.select :air @01:27:09.77 PS.seek 2.5ml @01:27:25.81 PSV.dial PTV @01:27:26.53 PTV.dial :puck @01:27:27.22 PBV.dial :waste1 @01:27:27.24 PS.seek 0ml @01:27:53.59 PSV.dial PSR @01:27:54.31 PSR.select :flush @01:27:54.43 PS.seek 0.5ml @01:28:00.53 PSR.select :air @01:28:00.64 PS.seek 2.5ml @01:28:21.72 PSV.dial PTV @01:28:22.44 PBV.dial PSV @01:28:22.97 PS.seek 0ml @01:28:49.22 PSV.dial PBV @01:28:49.68 PS.seek 2.5ml @01:29:15.81 PSV.dial :waste1 @01:29:16.50 PS.seek 0ml @01:29:42.68 PSV.dial PSR @01:29:43.25 PSR.select :air @01:29:43.27 PS.seek 2.5ml @01:30:09.46 PSV.dial PTV @01:30:10.18 PBV.dial PSV @01:30:10.20 PS.seek 0ml @01:30:36.40 PSV.dial PBV @01:30:36.84 PS.seek 2.5ml @01:31:02.96 PSV.dial :waste1 @01:31:03.66 PS.seek 0ml @01:31:29.80 PSV.dial PSR @01:31:30.24 PSR.select :flush @01:31:30.36 PS.seek 1ml @01:31:41.27 PSR.select :air @01:31:41.38 PS.seek 2.5ml @01:31:57.43 PSV.dial PTV @01:31:58.59 PTV.dial :puck @01:31:58.60 PBV.dial :waste1 @01:31:59.12 PS.seek 0ml @01:32:25.24 PSV.dial PSR @01:32:25.96 PSR.select :flush @01:32:26.08 PS.seek 0.5ml @01:32:32.02 PSR.select :air @01:32:32.14 PS.seek 2.5ml @01:32:53.27 PSV.dial PTV @01:32:53.99 PBV.dial PSV @01:32:54.53 PS.seek 0ml @01:33:20.99 PSV.dial PBV @01:33:21.45 PS.seek 2.5ml @01:33:47.68 PSV.dial :waste1 @01:33:48.39 PS.seek 0ml @01:34:14.61 PSV.dial PSR @01:34:15.05 PSR.select :air @01:34:15.08 PS.seek 2.5ml @01:34:41.33 PSV.dial PTV @01:34:42.04 PBV.dial PSV @01:34:42.06 PS.seek 0ml @01:35:08.30 PSV.dial PBV @01:35:08.76 PS.seek 2.5ml @01:35:34.92 PSV.dial :waste1 @01:35:35.63 PS.seek 0ml @01:36:01.86
Loading FlushPuck into Collection Clamp from Processing Clamp @01:36:02.03 Elbow.seek PC @01:36:04.58 Elbow.reconfigure :loose @01:36:04.67 Forearm.seek PC @01:36:05.59 Hand.close @01:36:06.14 PC.seek :open @01:36:10.09 Forearm.seek :retract @01:36:11.16 Elbow.reconfigure :tight @01:36:11.20 Elbow.seek CC @01:36:14.78 Elbow.reconfigure :loose @01:36:14.87 Forearm.reconfigure :tight @01:36:14.91 Forearm.seek CC @01:36:16.20 CC.seek :closed @01:36:20.42 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.79 @01:36:20.49 Hand.open @01:36:21.32 Forearm.reconfigure :loose @01:36:21.36 Forearm.seek :retract @01:36:22.36 Flushing the Collection Valve::Manifold @01:36:22.47 CSV.dial CSR @01:36:22.94 Pulling 5ml of CSR.flush @01:36:22.95 CSR.select :flush @01:36:23.07 CS.seek 5ml @01:36:36.53 delay 5 seconds @01:36:41.55 CSR.select :air @01:36:41.69 CS.reconfigure :fast @01:36:41.74 CS.seek 7.501ml @01:36:48.03 CSV.dial :waste @01:36:49.27 CS.reconfigure :default @01:36:49.31 CS.seek 0ml @01:37:09.00 Flushing lower manifold's waste1 port @01:37:09.11 CSV.dial :flush @01:37:09.55 CS.reconfigure :fast @01:37:09.59 CS.seek 5ml @01:37:21.25 delay 5 seconds @01:37:26.27 CSV.dial :air @01:37:26.76 CS.seek 7ml @01:37:32.03 CSV.dial CTV @01:37:32.06
CTV.dial CSV @01:37:33.37 CBV.dial :waste1 @01:37:34.10 CS.seek 0ml @01:37:50.03 Flushing lower manifold's waste2 port @01:37:50.14 CSV.dial :flush @01:37:50.86 CS.seek 5ml @01:38:02.50 delay 5 seconds @01:38:07.51 CSV.dial :air @01:38:08.01 CS.seek 7ml @01:38:13.18 CSV.dial CTV @01:38:13.21
CTV.dial CSV @01:38:13.65 CBV.dial :waste2 @01:38:15.13 CS.seek 0ml @01:38:31.09 Flushing lower manifold's waste2b port @01:38:31.20 CSV.dial :flush @01:38:31.92 CS.seek 5ml @01:38:43.68 delay 5 seconds @01:38:48.70 CSV.dial :air @01:38:49.21 CS.seek 6.999ml @01:38:54.50 CSV.dial CTV @01:38:54.52
CTV.dial CSV @01:38:54.95 CBV.dial :waste2b @01:38:55.94 CS.seek 0ml @01:39:11.87 Disinfecting external Intake with kill @01:39:11.98 CSV.dial CSR @01:39:12.47 Pulling 8ml of CSR.kill @01:39:12.48 CSR.select :kill @01:39:12.59 CS.seek 8ml @01:39:30.75 delay 5 seconds @01:39:35.76 CSR.select :air @01:39:35.88 CS.seek 10ml @01:39:41.06 CSV.dial CBV @01:39:41.09
CBV.dial CSV @01:39:42.12 CTV.dial :intake @01:39:42.84 Intake.select :open @01:39:42.94 CS.seek 2ml @01:40:01.02 Intake.select :close @01:40:01.12 CTV.dial :air @01:40:01.87 CS.reconfigure :default @01:40:01.91 CS.seek 0ml @01:40:07.80 CSV.dial CSR @01:40:08.84 Pulling 5ml of CSR.flush @01:40:08.85 CSR.select :flush @01:40:08.96 CS.seek 5ml @01:40:22.46 delay 5 seconds @01:40:27.48 CSR.select :air @01:40:27.59 CS.seek 10ml @01:40:41.00 CSV.dial CBV @01:40:41.98 CS.seek 0ml @01:41:07.99 Garaging FlushPuck in Collection Clamp @01:41:08.06 Elbow.reconfigure :tight @01:41:08.10 Elbow.seek CC @01:41:08.21 Elbow.reconfigure :loose @01:41:08.30 Forearm.seek CC @01:41:09.33 Hand.close @01:41:10.00 CC.seek :open @01:41:14.32 Forearm.seek :retract @01:41:15.36 Elbow.reconfigure :tight @01:41:15.40 Elbow.seek :garage @01:41:18.54 Elbow.reconfigure :loose @01:41:18.63 Forearm.reconfigure :tight @01:41:18.67 Forearm.seek :garage @01:41:19.81 Hand.open @01:41:20.56 Forearm.reconfigure :loose @01:41:20.60 Forearm.seek :retract @01:41:21.67 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 23:34:04 @01:41:21.72 Asleep after completing DA_WCR_120822 assays @01:41:21.74 GoodNews.email "{1 => 33,\n 2 => 11,\n 3 => 22,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@01:33:02, 22.6C, 35% humidity, 15.6psia, 14.050V, 0.300A, 0.294A avg, 4.21W\nSampled 200.0ml",Subject: "Asleep after completing DA_WCR_120822 assays" @01:41:56.28
Uploading Logs @01:43:27.11 upsync @20:19:15.53UTC09-Dec-22
ESP2 v3.41 I2C Gateway v3.20 for core bus @20:19:18.07 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @20:19:21.96 Polling Can every 10:00 @20:19:22.03 Cleanly exited at 20:16:33UTC09-Dec-22 @20:19:22.06 Loading /home/esp/esp2/mission/DA_WCR_120922.rb mission @20:19:22.11 GoodNews.email "Starting DA_WCR_120922 w/pucks in tube 3\n{1 => 33,\n 2 => 11,\n 3 => 22,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:19:21, 22.3C, 35% humidity, 15.6psia, 14.060V, 0.263A, 0.257A avg, 3.70W",Subject: "DA_WCR_120922 begun" @20:19:22.13
Preparing to sleep until + 00:00:05 @20:19:22.18 Asleep til + 00:00:05 @20:19:22.20 Continuing at 20:19 @20:19:22.48 Gate.power :core,:OFF @20:19:22.50 Gate.power :raw,:OFF Shutdown @20:19:26.77 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_120922.rb:14\n{1 => 33,\n 2 => 11,\n 3 => 22,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:19:22, 22.3C, 35% humidity, 15.6psia, 14.060V, 0.263A, 0.257A avg, 3.70W",Subject: "Asleep until 20:19" @20:19:27.18
Powering core bus @20:19:32.60 Gate.power :raw,:ON @20:19:33.71 CS.configure :default @20:19:34.01 PS.configure :default @20:19:34.24 SS.configure :default @20:19:34.42 CC.configure :default @20:19:34.57 PC.configure :default @20:19:34.87 Forearm.configure :loose @20:19:35.05 Elbow.configure :loose @20:19:35.30 SC.configure :loose @20:19:35.49 SE.configure :default @20:19:35.57 CH.coast @20:19:35.67 CH.configure :default @20:19:35.73 PH.coast @20:19:35.84 PH.configure :default @20:19:35.90 All dwarf controllers running firmware version 3.85 @20:19:36.06 Valve.reset @20:19:36.55 Intake.select :close @20:19:36.63 Exhaust.select :close @20:19:36.66 CTV.dial :air @20:19:36.92 PSV.dial :air @20:19:36.96 CSV.dial :waste1 @20:19:37.14
Forearm.reconfigure :loose @20:19:37.22 Forearm.home.seek :retract @20:19:37.54 SSV.dial :spit,avoiding: :exhaust @20:19:38.04 PS.reconfigure :default @20:19:38.15 PS.home.seek 0ml @20:19:38.21 Hand.close @20:19:38.25
Elbow.jog 1500 @20:19:38.53 CS.reconfigure :fast @20:19:38.61 SS.reconfigure :default @20:19:38.85 SS.home.seek 0ml @20:19:38.89 CS.home.seek 0ml @20:19:39.09
Elbow.reconfigure :loose @20:19:39.17 Elbow.home.seek :clear @20:19:43.78 SE.reconfigure :default @20:19:43.86 SE.home.seek :down @20:19:44.62 SC.reconfigure :tight @20:19:44.71 SC.home.seek :initial @20:19:47.05 PS.stop @20:20:14.80 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @20:20:14.82
Retry #1 of 3 @20:20:14.94 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @20:20:19.19 SC.configure :limp @20:20:19.29 Forearm.seek :retract @20:20:19.39 Elbow.seek :hammer @20:20:20.58 Forearm.reconfigure :tight @20:20:20.68 Forearm.seek retracted @20:20:21.12 PC.reconfigure :default @20:20:21.20
CC.reconfigure :default @20:20:21.36 CC.home.seek :open @20:20:21.40 PC.home.seek :open @20:20:25.25
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @20:20:25.27 Notice.email "At 20:20:24 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_120922.rb:14\n{1 => 33,\n 2 => 11,\n 3 => 22,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:20:25, 22.2C, 35% humidity, 15.6psia, 14.046V, 0.245A, 0.245A avg, 3.44W",Subject: "Started DA_WCR_120922 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @20:20:25.31
Starting initialPurge @20:20:25.37 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:20:25.51 Hand.open @20:20:26.52 Elbow.reconfigure :tight @20:20:26.59 Elbow.seek FlushPuck::Garage @20:20:28.50 Elbow.reconfigure :loose @20:20:28.70 Forearm.reconfigure :loose @20:20:28.85 Forearm.seek :garage @20:20:30.08 Hand.close @20:20:31.04 Forearm.seek :retract @20:20:32.12 Elbow.reconfigure :tight @20:20:32.20 Elbow.seek CC @20:20:35.18 Elbow.reconfigure :loose @20:20:35.38 Forearm.reconfigure :tight @20:20:35.47 Forearm.seek CC @20:20:37.14 CC.seek :closed @20:20:41.44 Collection Clamp onSpring/offSpring for 8/25 samples, on/off torque 1.88 @20:20:41.67 Hand.open @20:20:42.67 Forearm.reconfigure :loose @20:20:42.76 Forearm.seek :retract @20:20:43.82 Flushing the Collection Valve::Manifold @20:20:44.03 CSV.dial CSR @20:20:45.42 Pulling 5ml of CSR.flush @20:20:45.44 CSR.select :flush @20:20:45.60 CS.reconfigure :default @20:20:45.68 CS.seek 5ml @20:20:59.27 delay 5 seconds @20:21:04.28 CSR.select :air @20:21:04.51 CS.reconfigure :fast @20:21:04.59 CS.seek 7.501ml @20:21:10.92 CSV.dial :waste @20:21:12.20 CS.reconfigure :default @20:21:12.29 CS.seek 0ml @20:21:32.08 Purging external Intake @20:21:32.30 CSV.dial :flush @20:21:32.79 CS.reconfigure :fast @20:21:32.87 CS.seek 10ml @20:21:38.88 upsync @20:21:55.46
delay 5 seconds @20:22:00.48 CSV.dial CBV @20:22:00.52
CBV.dial CSV @20:22:01.33 CTV.dial :intake @20:22:02.07 Intake.select :open @20:22:02.17 CS.seek 0ml @20:22:24.61 Intake.select :close @20:22:24.71 CTV.dial :air @20:22:25.45 Flushing upper manifold @20:22:25.55 CSV.dial :flush @20:22:26.26 CS.seek 5ml @20:22:37.96 delay 5 seconds @20:22:42.98 CSV.dial :air @20:22:43.47 CS.seek 6.999ml @20:22:48.78 CSV.dial CBV @20:22:48.80
CBV.dial CSV @20:22:49.91 CTV.dial :air @20:22:49.93 CS.seek 0ml @20:23:05.89 delay 7 seconds @20:23:12.92 CS.seek 3ml @20:23:20.33 CSV.dial :waste @20:23:20.76 CS.seek 0ml @20:23:28.25 CSV.dial :flush @20:23:28.68 CS.seek 3ml @20:23:36.05 delay 5 seconds @20:23:41.07 CSV.dial :air @20:23:41.56 CS.seek 5ml @20:23:46.74 CSV.dial :air @20:23:46.76 CS.seek 0ml @20:23:58.46 delay 7 seconds @20:24:05.48 CS.seek 3ml @20:24:12.86 CSV.dial :waste @20:24:13.57 CS.seek 0ml @20:24:20.99 Garaging FlushPuck in Collection Clamp @20:24:21.06 Elbow.reconfigure :tight @20:24:21.10 Elbow.seek CC @20:24:21.21 Elbow.reconfigure :loose @20:24:21.29 Forearm.seek CC @20:24:22.26 Hand.close @20:24:22.86 CC.seek :open @20:24:27.22 Forearm.seek :retract @20:24:28.22 Elbow.reconfigure :tight @20:24:28.26 Elbow.seek :garage @20:24:31.24 Elbow.reconfigure :loose @20:24:31.32 Forearm.reconfigure :tight @20:24:31.37 Forearm.seek :garage @20:24:32.51 Hand.open @20:24:33.29 Forearm.reconfigure :loose @20:24:33.74 Forearm.seek :retract @20:24:34.81 da sampling at most 1000ml, wcr at most 1000ml @20:24:34.84 DAfans.email "Started da in tube 3 at 20:24:34UTC09-Dec-22\n{1 => 33,\n 2 => 11,\n 3 => 22,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:20:25, 22.2C, 35% humidity, 15.6psia, 14.046V, 0.245A, 0.245A avg, 3.44W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @20:24:34.87
Loading sh1 puck from tube 3 into Collection Clamp @20:24:35.10 SC.reconfigure :tight @20:24:35.14 SC.seek 3 @20:24:45.10 SC.configure :limp @20:24:45.48 Elbow.reconfigure :tight @20:24:45.58 Elbow.seek 3 @20:24:47.91 Forearm.reconfigure :tight @20:24:48.00 Forearm.seek 3 + 700 counts @20:24:49.08 SE.reconfigure :pick @20:24:49.18 SE.seek :beamBreak @20:24:50.94 SE.configure :default @20:24:51.24 Elbow.seek 3 @20:24:51.68 Forearm.seek 3 + 300 counts @20:24:51.95 Forearm.reconfigure :loose @20:24:52.03 Hand.close @20:24:52.66 SE.seek :down @20:24:53.07
Forearm.seek :retract @20:24:54.08 Elbow.seek CC @20:24:54.20 SE.stop @20:24:55.82
Elbow.reconfigure :loose @20:24:56.04 Forearm.reconfigure :tight @20:24:56.11 Forearm.seek CC @20:24:57.71 CC.seek :closed @20:25:02.08 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.70 @20:25:02.10 Hand.open @20:25:03.02 Forearm.reconfigure :loose @20:25:03.10 Forearm.seek :retract @20:25:04.14 collectSample 1000ml w/bubble point of 24psi @20:25:04.16 Priming 7ml @20:25:04.18 Intake.select :close @20:25:04.20 Exhaust.select :close @20:25:04.22 SS.hold @20:25:04.60 Calibrating pressure and temperature sensors @20:25:04.69 CTV.dial :air @20:25:04.75 SSV.dial :spit,avoiding: :exhaust @20:25:04.78 SS.reconfigure :fast @20:25:04.87 SS.seek 5ml @20:25:08.06 SSV.dial CBV @20:25:08.14
CBV.dial SSV @20:25:09.43 delay 3.8 seconds @20:25:13.45 SSV.dial :spit @20:25:14.78 SS.seek 0ml @20:25:17.56 SSV.dial CBV @20:25:18.89 CTV.dial :intake @20:25:19.66 SSV.dial CBV @20:25:19.72
CBV.dial SSV @20:25:19.74 Allowing 40s for 7.0ml of remaining 7.0ml @20:25:19.76 Intake.select :open @20:25:22.00 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:25:22.04 SS.reconfigure :inhaling 2.14ml/s @20:25:22.12 SS.seek 7ml @20:25:28.00 Stroke completed -- waiting for pressure to equalize ... @20:25:28.02 SS.reconfigure :exhale @20:25:28.10 SS.hold @20:25:32.78 Intake.select :close @20:25:33.02 SSV.dial :exhaust @20:25:33.81 Exhaust.select :open @20:25:33.95 SS.seek 0ml @20:25:37.81 Exhaust.select :close @20:25:37.96 Sampling 1000.0ml @20:25:37.97 Intake.select :close @20:25:37.99 Exhaust.select :close @20:25:38.03 SS.reconfigure :default @20:25:38.09 SS.hold @20:25:38.57 CTV.dial :intake @20:25:38.59 SSV.dial CBV @20:25:38.67
CBV.dial SSV @20:25:39.42 Allowing 130s for 25.0ml of remaining 1000.0ml @20:25:39.44 Intake.select :open @20:25:41.69 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:25:41.71 SS.reconfigure :inhaling 2.14ml/s @20:25:41.80 SS.seek 25ml @20:25:46.70 Vacuum too high -- waiting up to 40 seconds @20:25:46.71 SS.reconfigure :exhale @20:25:46.80 SS.hold @20:25:47.59 Intake @12.8psia, vacuum from 7.3 to 10.3psi @20:25:47.63 SS.reconfigure :inhaling 1.93ml/s @20:25:48.70 SS.seek 25ml @20:25:51.93 Vacuum too high -- waiting up to 40 seconds @20:25:51.95 SS.reconfigure :exhale @20:25:52.03 SS.hold @20:25:53.43 Intake @17.1psia, vacuum from 10.0 to 13.0psi @20:25:53.47 SS.reconfigure :inhaling 1.74ml/s @20:25:53.55 SS.seek 25ml @20:25:56.12 Vacuum too high -- waiting up to 40 seconds @20:25:56.14 SS.reconfigure :exhale @20:25:56.24 SS.hold @20:25:56.83 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:25:56.87 SS.reconfigure :inhaling 1.57ml/s @20:25:56.94 SS.seek 25ml @20:25:59.45 Vacuum too high -- waiting up to 40 seconds @20:25:59.47 SS.reconfigure :exhale @20:25:59.55 SS.hold @20:26:00.14 Intake @15.8psia, vacuum from 10.0 to 13.0psi @20:26:00.18 SS.reconfigure :inhaling 1.41ml/s @20:26:00.26 SS.seek 25ml @20:26:02.84 Vacuum too high -- waiting up to 40 seconds @20:26:02.86 SS.reconfigure :exhale @20:26:02.92 SS.hold @20:26:03.84 Intake @16.5psia, vacuum from 10.0 to 13.0psi @20:26:03.87 SS.reconfigure :inhaling 1.27ml/s @20:26:03.97 SS.seek 25ml @20:26:06.56 Vacuum too high -- waiting up to 40 seconds @20:26:06.58 SS.reconfigure :exhale @20:26:06.66 SS.hold @20:26:07.27 Intake @16.2psia, vacuum from 10.0 to 13.0psi @20:26:07.30 SS.reconfigure :inhaling 1.14ml/s @20:26:07.38 SS.seek 25ml @20:26:09.40 Vacuum too high -- waiting up to 40 seconds @20:26:09.42 SS.reconfigure :exhale @20:26:09.48 SS.hold @20:26:10.08 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:26:10.12 SS.reconfigure :inhaling 1.03ml/s @20:26:10.20 SS.seek 25ml @20:26:12.36 Vacuum too high -- waiting up to 40 seconds @20:26:12.38 SS.reconfigure :exhale @20:26:12.46 SS.hold @20:26:12.93 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:26:12.97 SS.reconfigure :inhaling 0.93ml/s @20:26:13.07 SS.seek 25ml @20:26:16.48 Vacuum too high -- waiting up to 40 seconds @20:26:16.50 SS.reconfigure :exhale @20:26:16.58 SS.hold @20:26:17.18 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:26:17.22 SS.reconfigure :inhaling 0.84ml/s @20:26:17.29 SS.seek 25ml @20:26:21.26 Vacuum too high -- waiting up to 40 seconds @20:26:21.28 SS.reconfigure :exhale @20:26:21.36 SS.hold @20:26:21.94 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:26:21.98 SS.reconfigure :inhaling 0.76ml/s @20:26:22.09 SS.seek 25ml @20:26:28.84 Stroke completed -- waiting for pressure to equalize ... @20:26:28.86 SS.reconfigure :exhale @20:26:28.97 SS.hold @20:26:30.41 Intake.select :close @20:26:30.65 SSV.dial :exhaust @20:26:31.42 Exhaust.select :open @20:26:31.60 SS.seek 0ml @20:26:40.43 Exhaust.select :close @20:26:40.61 SSV.dial CBV @20:26:40.65
CBV.dial SSV @20:26:41.47 Allowing 130s for 25.0ml of remaining 975.0ml @20:26:41.49 Intake.select :open @20:26:43.73 Intake @15.5psia, vacuum from 10.0 to 13.0psi @20:26:43.77 SS.reconfigure :inhaling 0.76ml/s @20:26:43.94 SS.seek 25ml @20:26:55.85 upsync @20:27:00.94
Vacuum too high -- waiting up to 40 seconds @20:27:00.95 SS.reconfigure :exhale @20:27:00.99 SS.hold @20:27:01.64 Intake @15.2psia, vacuum from 9.7 to 12.7psi @20:27:01.65 SS.reconfigure :inhaling 0.69ml/s @20:27:01.70 SS.seek 25ml @20:27:07.97 Vacuum too high -- waiting up to 40 seconds @20:27:07.98 SS.reconfigure :exhale @20:27:08.02 SS.hold @20:27:08.67 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:27:08.68 SS.reconfigure :inhaling 0.61ml/s @20:27:08.73 SS.seek 25ml @20:27:25.11 Stroke completed -- waiting for pressure to equalize ... @20:27:25.12 SS.reconfigure :exhale @20:27:25.17 SS.hold @20:27:27.20 Intake.select :close @20:27:27.33 SSV.dial :exhaust @20:27:28.05 Exhaust.select :open @20:27:28.16 SS.seek 0ml @20:27:37.01 Exhaust.select :close @20:27:37.11 SSV.dial CBV @20:27:37.16
CBV.dial SSV @20:27:37.85 Allowing 130s for 25.0ml of remaining 950.0ml @20:27:37.86 Intake.select :open @20:27:40.01 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:27:40.03 SS.reconfigure :inhaling 0.61ml/s @20:27:40.07 SS.seek 25ml @20:28:23.60 Stroke completed -- waiting for pressure to equalize ... @20:28:23.61 SS.reconfigure :exhale @20:28:23.65 SS.hold @20:28:26.04 Intake.select :close @20:28:26.18 SSV.dial :exhaust @20:28:26.91 Exhaust.select :open @20:28:27.02 SS.seek 0ml @20:28:35.94 Exhaust.select :close @20:28:36.04 SSV.dial CBV @20:28:36.07
CBV.dial SSV @20:28:36.82 Allowing 130s for 25.0ml of remaining 925.0ml @20:28:36.83 Intake.select :open @20:28:38.98 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:28:39.00 SS.reconfigure :inhaling 0.61ml/s @20:28:39.04 SS.seek 25ml @20:29:05.34 Vacuum too high -- waiting up to 40 seconds @20:29:05.35 SS.reconfigure :exhale @20:29:05.39 SS.hold @20:29:06.66 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:29:06.68 SS.reconfigure :inhaling 0.56ml/s @20:29:06.72 SS.seek 25ml @20:29:20.21 Vacuum too high -- waiting up to 40 seconds @20:29:20.22 SS.reconfigure :exhale @20:29:20.26 SS.hold @20:29:21.53 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:29:21.55 SS.reconfigure :inhaling 0.50ml/s @20:29:21.59 SS.seek 25ml @20:29:27.59 Stroke completed -- waiting for pressure to equalize ... @20:29:27.60 SS.reconfigure :exhale @20:29:27.65 SS.hold @20:29:30.03 Intake.select :close @20:29:30.17 SSV.dial :exhaust @20:29:30.89 Exhaust.select :open @20:29:31.00 SS.seek 0ml @20:29:40.40 Exhaust.select :close @20:29:40.50 SSV.dial CBV @20:29:40.53
CBV.dial SSV @20:29:41.27 Allowing 130s for 25.0ml of remaining 900.0ml @20:29:41.28 Intake.select :open @20:29:43.43 Intake @15.2psia, vacuum from 9.7 to 12.7psi @20:29:43.45 SS.reconfigure :inhaling 0.50ml/s @20:29:43.49 SS.seek 25ml @20:30:22.52 Vacuum too high -- waiting up to 40 seconds @20:30:22.53 SS.reconfigure :exhale @20:30:22.57 SS.hold @20:30:24.10 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:30:24.12 SS.reconfigure :inhaling 0.46ml/s @20:30:24.16 SS.seek 25ml @20:30:39.51 Stroke completed -- waiting for pressure to equalize ... @20:30:39.52 SS.reconfigure :exhale @20:30:39.56 SS.hold @20:30:43.62 Intake.select :close @20:30:43.76 SSV.dial :exhaust @20:30:44.48 Exhaust.select :open @20:30:44.59 SS.seek 0ml @20:30:53.50 Exhaust.select :close @20:30:53.60 SSV.dial CBV @20:30:53.64
CBV.dial SSV @20:30:54.36 Allowing 130s for 25.0ml of remaining 875.0ml @20:30:54.37 Intake.select :open @20:30:56.52 Intake @15.2psia, vacuum from 9.7 to 12.7psi @20:30:56.54 SS.reconfigure :inhaling 0.46ml/s @20:30:56.58 SS.seek 25ml @20:31:55.11 Vacuum too high -- waiting up to 40 seconds @20:31:55.12 SS.reconfigure :exhale @20:31:55.16 SS.hold @20:31:58.04 Intake @15.0psia, vacuum from 9.5 to 12.5psi @20:31:58.06 SS.reconfigure :inhaling 0.41ml/s @20:31:58.10 SS.seek 25ml @20:31:59.90 Stroke completed -- waiting for pressure to equalize ... @20:31:59.91 SS.reconfigure :exhale @20:31:59.95 SS.hold @20:32:04.01 Intake.select :close @20:32:04.15 SSV.dial :exhaust @20:32:04.87 Exhaust.select :open @20:32:04.98 SS.seek 0ml @20:32:14.34 Exhaust.select :close @20:32:14.44 SSV.dial CBV @20:32:14.47
CBV.dial SSV @20:32:15.22 Allowing 130s for 25.0ml of remaining 850.0ml @20:32:15.23 Intake.select :open @20:32:17.38 Intake @15.1psia, vacuum from 9.6 to 12.6psi @20:32:17.39 SS.reconfigure :inhaling 0.41ml/s @20:32:17.44 SS.seek 25ml @20:32:57.67 Vacuum too high -- waiting up to 40 seconds @20:32:57.68 SS.reconfigure :exhale @20:32:57.72 SS.hold @20:33:00.59 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:33:00.61 SS.reconfigure :inhaling 0.37ml/s @20:33:00.65 SS.seek 25ml @20:33:25.77 Vacuum too high -- waiting up to 40 seconds @20:33:25.78 SS.reconfigure :exhale @20:33:25.82 SS.hold @20:33:28.29 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:33:28.31 SS.reconfigure :inhaling 0.33ml/s @20:33:28.35 SS.seek 25ml @20:33:33.38 Stroke completed -- waiting for pressure to equalize ... @20:33:33.39 SS.reconfigure :exhale @20:33:33.43 SS.hold @20:33:37.65 Intake.select :close @20:33:37.78 SSV.dial :exhaust @20:33:38.51 Exhaust.select :open @20:33:38.62 SS.seek 0ml @20:33:47.88 Exhaust.select :close @20:33:47.99 SSV.dial CBV @20:33:48.01
CBV.dial SSV @20:33:48.76 Allowing 130s for 25.0ml of remaining 825.0ml @20:33:48.77 Intake.select :open @20:33:50.92 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:33:50.94 SS.reconfigure :inhaling 0.33ml/s @20:33:50.98 SS.seek 25ml @20:35:17.25 Stroke completed -- waiting for pressure to equalize ... @20:35:17.26 SS.reconfigure :exhale @20:35:17.31 SS.hold @20:35:25.83 Intake.select :close @20:35:25.96 SSV.dial :exhaust @20:35:26.69 Exhaust.select :open @20:35:26.80 SS.seek 0ml @20:35:35.77 Exhaust.select :close @20:35:35.87 SSV.dial CBV @20:35:35.90
CBV.dial SSV @20:35:36.63 Allowing 130s for 25.0ml of remaining 800.0ml @20:35:36.64 Intake.select :open @20:35:38.79 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:35:38.81 SS.reconfigure :inhaling 0.33ml/s @20:35:38.85 SS.seek 25ml @20:36:39.64 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @20:36:39.68 SS.hold @20:36:43.73 Intake @15.0psia, vacuum from 9.5 to 12.5psi @20:36:43.75 SS.reconfigure :inhaling 0.30ml/s @20:36:43.79 SS.seek 25ml @20:37:10.76 Vacuum too high -- waiting up to 40 seconds @20:37:10.77 SS.reconfigure :exhale @20:37:10.81 SS.hold @20:37:16.44 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:37:16.46 SS.reconfigure :inhaling 0.27ml/s @20:37:16.50 SS.seek 25ml @20:37:18.36 Stroke completed -- waiting for pressure to equalize ... @20:37:18.37 SS.reconfigure :exhale @20:37:18.41 SS.hold @20:37:24.81 Intake.select :close @20:37:24.95 SSV.dial :exhaust @20:37:25.67 Exhaust.select :open @20:37:25.78 SS.seek 0ml @20:37:34.95 Exhaust.select :close @20:37:35.06 SSV.dial CBV @20:37:35.08
CBV.dial SSV @20:37:35.83 Allowing 130s for 25.0ml of remaining 775.0ml @20:37:35.84 Intake.select :open @20:37:37.99 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:37:38.01 SS.reconfigure :inhaling 0.27ml/s @20:37:38.05 SS.seek 25ml @20:39:19.57 Vacuum too high -- waiting up to 40 seconds @20:39:19.58 SS.reconfigure :exhale @20:39:19.62 SS.hold @20:39:27.05 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:39:27.07 SS.reconfigure :inhaling 0.24ml/s @20:39:27.11 SS.seek 25ml @20:39:33.79 Stroke completed -- waiting for pressure to equalize ... @20:39:33.80 SS.reconfigure :exhale @20:39:33.84 SS.hold @20:39:42.39 Intake.select :close @20:39:42.53 SSV.dial :exhaust @20:39:43.25 Exhaust.select :open @20:39:43.36 SS.seek 0ml @20:39:52.29 Exhaust.select :close @20:39:52.40 SSV.dial CBV @20:39:52.42
CBV.dial SSV @20:39:53.17 Allowing 130s for 25.0ml of remaining 750.0ml @20:39:53.18 Intake.select :open @20:39:55.33 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:39:55.35 SS.reconfigure :inhaling 0.24ml/s @20:39:55.39 SS.seek 25ml @20:41:13.94 Vacuum too high -- waiting up to 40 seconds @20:41:13.95 SS.reconfigure :exhale @20:41:13.99 SS.hold @20:41:17.41 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:41:17.43 SS.reconfigure :inhaling 0.21ml/s @20:41:17.47 SS.seek 25ml @20:42:03.20 SS.reconfigure :default @20:42:03.23 SS.hold @20:42:03.45 Intake.select :close @20:42:03.59 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 274.6ml @20:42:03.60 SSV.dial :exhaust @20:42:04.34 Exhaust.select :open @20:42:04.44 SS.reconfigure :exhale @20:42:04.48 SS.seek 0ml @20:42:13.34 Exhaust.select :close @20:42:13.44 Sampled 274.6ml @20:42:13.45 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @20:42:13.46 Intake.select :close @20:42:13.52 CSV.dial :waste @20:42:13.56 CS.seek 0ml @20:42:13.66 CSV.dial :air @20:42:13.68 SSV.dial CBV,avoiding: :exhaust @20:42:13.71
CBV.dial SSV @20:42:14.45 CS.seek 10ml @20:42:15.54
CTV.dial :air @20:42:16.29 delay 15 seconds @20:42:31.30 SSV.dial :spit,avoiding: :exhaust @20:42:32.61 CTV.dial CSV,avoiding: :intake @20:42:32.65
SS.seek 0ml @20:42:32.76 SSV.dial CBV,avoiding: :exhaust @20:42:37.32 CBV.dial between SSV and waste2 @20:42:37.69 CTV.dial CSV,avoiding: :air @20:42:37.72
CSV.dial CTV @20:42:38.15 CS.reconfigure :pressurize @20:42:38.19 CS.seek 0ml @20:42:56.51 BubblePoint: Intake @40.2psia, Outlet @16.0psia @20:42:56.52 CBV.dial SSV @20:42:56.85 SS.reconfigure :default @20:42:56.89 SS.seek 10ml @20:43:02.55 delay 15 seconds @20:43:17.66 Evacuated: Intake @16.2psia, Outlet @10.1psia @20:43:17.68 SSV.dial :spit,avoiding: :exhaust @20:43:18.97 SS.reconfigure :exhale @20:43:19.01 SS.seek 0ml @20:43:24.44 SS.reconfigure :fast @20:43:24.48 SS.seek 5ml @20:43:27.29 SSV.dial CBV,avoiding: :exhaust @20:43:27.32
CBV.dial SSV @20:43:28.60 CTV.dial :air,avoiding: :intake @20:43:29.88 CSV.dial CTV @20:43:29.91
SS.seek 10ml @20:43:30.91 CSV.dial :air @20:43:31.37 CS.reconfigure :fast @20:43:31.41 CS.seek 0ml @20:43:32.60
SSV.dial :spit,avoiding: :exhaust @20:43:33.90 SS.reconfigure :exhale @20:43:33.94 SS.seek 0ml @20:43:38.67 CS.stop @20:43:39.05
SS.reconfigure :fast @20:43:39.09 SS.seek 5ml @20:43:41.88 SSV.dial CBV,avoiding: :exhaust @20:43:41.91
CBV.dial SSV @20:43:44.22 SSV.dial :spit,avoiding: :exhaust @20:43:45.51 SS.reconfigure :default @20:43:45.55 SS.seek 0ml @20:43:48.64 SS.stop @20:43:48.68 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @20:43:48.69 Intake.select :close @20:43:48.76 CSV.dial :waste @20:43:48.87 SSV.dial CBV,avoiding: :exhaust @20:43:48.89
CBV.dial SSV @20:43:49.53 CS.seek 0ml @20:43:49.59 CSV.dial :air @20:43:50.18
CTV.dial :air @20:43:50.19 delay 15 seconds @20:43:50.76 CS.seek 10ml @20:44:05.21
SSV.dial :spit,avoiding: :exhaust @20:44:06.51 CTV.dial CSV,avoiding: :intake @20:44:06.54
SS.reconfigure :exhale @20:44:06.63 SS.seek 0ml @20:44:06.70 SSV.dial CBV,avoiding: :exhaust @20:44:13.38 CBV.dial between SSV and waste2 @20:44:13.77 CTV.dial CSV,avoiding: :air @20:44:13.80
CSV.dial CTV @20:44:14.24 CS.reconfigure :pressurize @20:44:14.28 CS.seek 0ml @20:44:35.61 BubblePoint: Intake @39.3psia, Outlet @15.1psia @20:44:35.62 CBV.dial SSV @20:44:35.94 SS.reconfigure :default @20:44:35.99 SS.seek 10ml @20:44:41.46 delay 15 seconds @20:44:56.56 Evacuated: Intake @18.1psia, Outlet @12.3psia @20:44:56.58 SSV.dial :spit,avoiding: :exhaust @20:44:57.87 SS.reconfigure :exhale @20:44:57.91 SS.seek 0ml @20:45:03.62 SS.reconfigure :fast @20:45:03.66 SS.seek 5ml @20:45:06.26 SSV.dial CBV,avoiding: :exhaust @20:45:06.30
CBV.dial SSV @20:45:07.55 CTV.dial :air,avoiding: :intake @20:45:08.83 CSV.dial CTV @20:45:08.87
SS.seek 10ml @20:45:09.86 CSV.dial :air @20:45:10.30 CS.reconfigure :fast @20:45:10.35 CS.seek 0ml @20:45:11.35
SSV.dial :spit,avoiding: :exhaust @20:45:12.66 SS.reconfigure :exhale @20:45:12.71 SS.seek 0ml @20:45:15.07 CS.stop @20:45:18.36
SS.reconfigure :fast @20:45:18.40 SS.seek 5ml @20:45:20.94 SSV.dial CBV,avoiding: :exhaust @20:45:20.97
CBV.dial SSV @20:45:23.26 SSV.dial :spit,avoiding: :exhaust @20:45:24.55 SS.reconfigure :default @20:45:24.60 SS.seek 0ml @20:45:28.17 SS.stop @20:45:28.21 Lysing: Adding 2.0ml AqMeOH @20:45:28.22 CBV.dial between CSV and waste1,avoiding: :DBV @20:45:29.66 CTV.dial CSV @20:45:30.94 CSV.dial CSR @20:45:31.71 Pulling 1ml of CSR.AqMeOH CSR.select :AqMeOH @20:45:31.82 CS.reconfigure :default @20:45:31.86 CS.seek 1ml @20:45:35.23 delay 5 seconds @20:45:40.25 CSR.select :air @20:45:40.40 CS.seek 5.999ml @20:45:53.73 delay 2 seconds @20:45:55.75 CSV.dial :waste2 @20:45:56.19 CS.reconfigure :fast @20:45:56.23 CS.seek 0ml @20:46:10.01 CSV.dial CSR @20:46:10.61 Pulling 1.999ml of CSR.AqMeOH @20:46:10.62 CSR.select :AqMeOH @20:46:10.73 CS.reconfigure :default @20:46:10.77 CS.seek 2ml @20:46:16.67 delay 5 seconds @20:46:21.69 CSR.select :air @20:46:21.84 CS.seek 5ml @20:46:30.23 delay 2 seconds @20:46:32.25 CSV.dial CTV @20:46:32.69 CS.seek 1ml @20:46:43.70 Before lysing Collection Puck Heater at 16.6C/38.8psia @20:46:43.72 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @20:46:43.74 CH.seek 70C,8:00 @20:48:31.01 CH.hold 10:00,70C @20:48:31.18 Begun lysing Collection Puck Heater at 68C/43.1psia @20:58:31.19 Ended lysing Collection Puck Heater at 70C/44.6psia @20:58:31.21 CS.seek 0ml @20:58:34.56 CTV.dial between spare and air,avoiding: :intake @20:58:35.56 CSV.dial :air @20:58:36.00 CS.seek 5ml @20:58:49.40 CSV.dial CTV @20:58:49.95 delay 2 seconds @20:58:51.96 CSV.dial :air @20:58:52.51 delay 2 seconds @20:58:54.52 CSV.dial CTV @20:58:54.95 delay 2 seconds @20:58:56.96 CSV.dial :air @20:58:57.40 delay 2 seconds @20:58:59.42 CS.seek 0ml @20:59:12.89 Puck @71.5psia after final lysis pressurization @20:59:12.90 Recovering Lysate into Collection syringe @20:59:12.91 CSV.dial CBV @20:59:13.90 CBV.dial CSV @20:59:14.27 CS.seek 5.001ml @20:59:27.74 delay 5 seconds @20:59:32.76 CTV.dial :air @20:59:33.27 CS.seek 7.001ml @20:59:39.11 delay 5 seconds @20:59:44.13 CSV.dial :air2 @20:59:44.57 CS.seek 10ml @20:59:52.93 CSV.dial between air and CTV,avoiding: CBV @20:59:54.04 Lysing Complete @20:59:54.11 Storing sh1 puck in Collection Clamp to tube 2 @20:59:54.26 SC.reconfigure :tight @20:59:54.31 SC.seek 2 @20:59:57.86 SC.configure :limp @20:59:58.06 Elbow.reconfigure :tight @20:59:58.10 Elbow.seek 2 @20:59:59.83 Forearm.reconfigure :tight @20:59:59.87 Forearm.seek 2 + 700 counts @21:00:00.92 SE.reconfigure :pick @21:00:00.97 SE.seek :beamBreak @21:00:03.34 SE.configure :default @21:00:03.48 Elbow.reconfigure :limp @21:00:03.52 Forearm.reconfigure :loose @21:00:03.56 Forearm.seek 2 @21:00:03.97 Elbow.hold @21:00:04.03 Elbow.reconfigure :tight @21:00:04.11 SE.seek 10.25 Pucks below Top Plate @21:00:04.56 Forearm.force -20 @21:00:04.60 Forearm.seek 2 + 312 counts @21:00:04.88 Forearm.reconfigure :tight @21:00:04.92 Forearm.seek 2 + 700 counts @21:00:05.26 SE.seek 11.25 Pucks below Top Plate @21:00:05.83 Forearm.reconfigure :loose @21:00:05.87 Forearm.seek :retract @21:00:06.79 Elbow.seek CC @21:00:08.42 Elbow.reconfigure :loose @21:00:08.51 Forearm.seek CC @21:00:09.44 Hand.close @21:00:10.16 CC.seek :open @21:00:14.57 Forearm.seek :retract @21:00:16.08 Elbow.reconfigure :tight @21:00:16.12 Elbow.seek 2 @21:00:17.80 Elbow.reconfigure :limp @21:00:17.84 Forearm.seek 2 @21:00:18.95 Elbow.hold @21:00:19.02 Elbow.reconfigure :tight @21:00:19.06 Hand.open @21:00:19.89 Forearm.force -20 @21:00:19.93 Forearm.seek 2 + 317 counts @21:00:20.24 Forearm.reconfigure :tight @21:00:20.28 Forearm.seek 2 + 700 counts @21:00:20.63 SE.seek :down @21:00:21.76
Forearm.reconfigure :loose @21:00:21.80 Forearm.seek :retract @21:00:22.67 SE.stop @21:00:22.79
Tranferring DA @21:00:22.80 Beginning samplePassC @21:00:22.81 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:00:22.99 Elbow.seek FlushPuck::Garage @21:00:24.91 Elbow.reconfigure :loose @21:00:24.99 Forearm.seek :garage @21:00:26.11 Hand.close @21:00:26.77 Forearm.seek :retract @21:00:27.83 Elbow.reconfigure :tight @21:00:27.87 Elbow.seek CC @21:00:30.73 Elbow.reconfigure :loose @21:00:30.82 Forearm.reconfigure :tight @21:00:30.86 Forearm.seek CC @21:00:32.12 CC.seek :closed @21:00:36.35 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.89 @21:00:36.42 Hand.open @21:00:37.21 Forearm.reconfigure :loose @21:00:37.26 Forearm.seek :retract @21:00:38.33 PSV.dial PSR @21:00:39.64 Pulling 1.5ml of PSR.flush @21:00:39.65 PSR.select :flush @21:00:39.77 PS.seek 1.5ml @21:00:55.82 delay 5 seconds @21:01:00.84 PSV.dial :waste1 @21:01:01.28 PS.seek 0ml @21:01:17.44 PSV.dial PSR @21:01:17.88 PSR.select :air @21:01:17.99 PS.seek 2.5ml @21:01:44.14 PRV.dial CBV,avoiding: :ab2 @21:01:44.16
CBV.dial PRV @21:01:45.79 PSV.dial :mixloop @21:01:46.77 CTV.dial :air,avoiding: :intake @21:01:46.79 PTV.dial :PRVmixing @21:01:48.01 PS.seek 0ml @21:02:14.20 PSV.dial :air @21:02:14.64 PS.seek 2.5ml @21:02:40.85 PSV.dial :mixloop @21:02:41.29 PS.seek 0ml @21:03:07.41 PSV.dial :air @21:03:07.85 PS.seek 2.5ml @21:03:34.00 PSV.dial :mixloop @21:03:34.45 PS.seek 0ml @21:04:00.66 Garaging FlushPuck in Collection Clamp @21:04:00.73 Elbow.reconfigure :tight @21:04:00.77 Elbow.seek CC @21:04:00.87 Elbow.reconfigure :loose @21:04:00.96 Forearm.seek CC @21:04:01.88 Hand.close @21:04:02.53 CC.seek :open @21:04:06.87 Forearm.seek :retract @21:04:08.36 Elbow.reconfigure :tight @21:04:08.40 Elbow.seek :garage @21:04:11.56 Elbow.reconfigure :loose @21:04:11.65 Forearm.reconfigure :tight @21:04:11.69 Forearm.seek :garage @21:04:12.77 Hand.open @21:04:13.59 Forearm.reconfigure :loose @21:04:13.63 Forearm.seek :retract @21:04:14.66 PSV.dial :mixloop @21:04:14.68 PTV.dial :PRVmixing @21:04:14.69 PRV.dial CBV @21:04:14.71
CBV.dial PRV @21:04:14.73 CTV.dial :air,avoiding: :intake @21:04:14.75 Loading lyfil puck from tube 3 into Collection Clamp @21:04:14.97 SC.reconfigure :tight @21:04:15.01 SC.seek 3 @21:04:18.55 SC.configure :limp @21:04:18.74 Elbow.reconfigure :tight @21:04:18.78 Elbow.seek 3 @21:04:20.67 Forearm.reconfigure :tight @21:04:20.71 Forearm.seek 3 + 700 counts @21:04:21.82 SE.reconfigure :pick @21:04:21.87 SE.seek :beamBreak @21:04:23.61 SE.configure :default @21:04:23.76 Elbow.seek 3 @21:04:23.94 Forearm.seek 3 + 300 counts @21:04:24.16 Forearm.reconfigure :loose @21:04:24.20 Hand.close @21:04:24.79 SE.seek :down @21:04:24.98
Forearm.seek :retract @21:04:26.01 Elbow.seek CC @21:04:26.28 SE.stop @21:04:27.61
Elbow.reconfigure :loose @21:04:27.70 Forearm.reconfigure :tight @21:04:27.74 Forearm.seek CC @21:04:29.02 CC.seek :closed @21:04:33.25 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.62 @21:04:33.26 Hand.open @21:04:34.09 Forearm.reconfigure :loose @21:04:34.13 Forearm.seek :retract @21:04:35.21 CTV.dial CSV,avoiding: :intake @21:04:36.48 CSV.dial CTV @21:04:36.89 PS.seek 2.5ml @21:05:03.22 CS.seek 5.5ml @21:05:15.36 delay 8 seconds @21:05:23.38 PSV.dial :air @21:05:23.83 delay 2 seconds @21:05:25.87 PSV.dial :waste2 @21:05:26.59 PS.seek 0ml @21:05:52.78 CBV.dial between PRV and waste1 @21:05:53.09 CSV.dial :air @21:05:53.64 CS.seek 10ml @21:06:05.79 CSV.dial CTV @21:06:06.20 CS.seek 0ml @21:06:32.23 CBV.dial :waste1 @21:06:32.53 delay 5 seconds @21:06:37.55 CTV.dial :air,avoiding: :intake @21:06:38.88 Storing lyfil puck in Collection Clamp to tube 2 @21:06:39.04 SC.reconfigure :tight @21:06:39.08 SC.seek 2 @21:06:42.65 SC.configure :limp @21:06:42.85 Elbow.reconfigure :tight @21:06:42.90 Elbow.seek 2 @21:06:44.69 Forearm.reconfigure :tight @21:06:45.21 Forearm.seek 2 + 700 counts @21:06:46.24 SE.reconfigure :pick @21:06:46.28 SE.seek :beamBreak @21:06:48.68 SE.configure :default @21:06:48.82 Elbow.reconfigure :limp @21:06:48.87 Forearm.reconfigure :loose @21:06:48.91 Forearm.seek 2 @21:06:49.32 Elbow.hold @21:06:49.38 Elbow.reconfigure :tight @21:06:49.46 SE.seek 11.26 Pucks below Top Plate @21:06:50.05 Forearm.force -20 @21:06:50.09 Forearm.seek 2 + 313 counts @21:06:50.40 Forearm.reconfigure :tight @21:06:50.44 Forearm.seek 2 + 700 counts @21:06:50.82 SE.seek 12.26 Pucks below Top Plate @21:06:51.38 Forearm.reconfigure :loose @21:06:51.42 Forearm.seek :retract @21:06:52.35 Elbow.seek CC @21:06:53.98 Elbow.reconfigure :loose @21:06:54.07 Forearm.seek CC @21:06:55.05 Hand.close @21:06:55.65 CC.seek :open @21:07:00.07 Forearm.seek :retract @21:07:01.18 Elbow.reconfigure :tight @21:07:01.22 Elbow.seek 2 @21:07:03.13 Elbow.reconfigure :limp @21:07:03.17 Forearm.seek 2 @21:07:04.27 Elbow.hold @21:07:04.33 Elbow.reconfigure :tight @21:07:04.37 Hand.open @21:07:05.26 Forearm.force -20 @21:07:05.29 Forearm.seek 2 + 316 counts @21:07:05.66 Forearm.reconfigure :tight @21:07:05.71 Forearm.seek 2 + 700 counts @21:07:05.97 SE.seek :down @21:07:07.10
Forearm.reconfigure :loose @21:07:07.14 Forearm.seek :retract @21:07:07.85 SE.stop @21:07:08.07
PSV.dial :air @21:07:08.79 PS.seek 2.5ml @21:07:34.90 delay 1 second @21:07:35.92 PSV.dial :mixloop @21:07:36.34 delay 1 second @21:07:37.36 PSV.dial :air @21:07:37.78 delay 1 second @21:07:38.79 PSV.dial :mixloop @21:07:39.22 delay 1 second @21:07:40.23 PSV.dial :air @21:07:40.69 delay 1 second @21:07:41.71 PS.seek 0ml @21:08:07.90 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @21:08:08.08 Elbow.seek FlushPuck::Garage @21:08:09.87 Elbow.reconfigure :loose @21:08:09.96 Forearm.seek :garage @21:08:11.08 Hand.close @21:08:11.75 Forearm.seek :retract @21:08:12.82 Elbow.reconfigure :tight @21:08:12.86 Elbow.seek PC @21:08:14.16 Elbow.reconfigure :loose @21:08:14.25 Forearm.reconfigure :tight @21:08:14.29 Forearm.seek PC @21:08:15.59 PC.seek :closed @21:08:19.99 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.41 @21:08:20.11 Hand.open @21:08:20.95 Forearm.reconfigure :loose @21:08:20.99 Forearm.seek :retract @21:08:22.06 PRV.dial :air @21:08:22.51 PSV.dial :mixloop @21:08:23.03 PS.seek 0.3ml @21:08:26.84 PSV.dial PSR @21:08:27.86 Pulling 0.4ml of PSR.air @21:08:27.87 PSR.select :air @21:08:27.89 PS.seek 0.7ml @21:08:32.75 delay 3 seconds @21:08:35.77 PSR.select :air @21:08:35.79 PS.seek 2.5ml @21:08:54.84 delay 3 seconds @21:08:57.85 PSV.dial :mixloop @21:08:58.84 PTV.dial :mixing @21:08:59.54 PBV.dial :waste2 @21:09:00.43 PS.seek 0ml @21:09:26.68 Garaging FlushPuck in Processing Clamp @21:09:26.76 Elbow.reconfigure :tight @21:09:26.80 Elbow.seek PC @21:09:26.91 Elbow.reconfigure :loose @21:09:27.00 Forearm.seek PC @21:09:28.07 Hand.close @21:09:28.64 PC.seek :open @21:09:32.60 Forearm.seek :retract @21:09:33.67 Elbow.reconfigure :tight @21:09:33.71 Elbow.seek :garage @21:09:35.07 Elbow.reconfigure :loose @21:09:35.16 Forearm.reconfigure :tight @21:09:35.20 Forearm.seek :garage @21:09:36.39 Hand.open @21:09:37.20 Forearm.reconfigure :loose @21:09:37.24 Forearm.seek :retract @21:09:38.34 Loading da puck from tube 3 into Processing Clamp @21:09:38.56 SC.reconfigure :tight @21:09:38.60 SC.seek 3 @21:09:42.16 SC.configure :limp @21:09:42.36 Elbow.reconfigure :tight @21:09:42.40 Elbow.seek 3 @21:09:44.34 Forearm.reconfigure :tight @21:09:44.38 Forearm.seek 3 + 700 counts @21:09:45.37 SE.reconfigure :pick @21:09:45.42 SE.seek :beamBreak @21:09:47.27 SE.configure :default @21:09:47.42 Elbow.seek 3 @21:09:47.59 Forearm.seek 3 + 300 counts @21:09:47.86 Forearm.reconfigure :loose @21:09:47.90 Hand.close @21:09:48.53 SE.seek :down @21:09:48.72
Forearm.seek :retract @21:09:49.74 Elbow.seek PC @21:09:50.00 SE.stop @21:09:52.47
Elbow.reconfigure :loose @21:09:52.56 Forearm.reconfigure :tight @21:09:52.61 Forearm.seek PC @21:09:53.95 PC.seek :closed @21:09:57.85 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.37 @21:09:57.86 Hand.open @21:09:58.69 Forearm.reconfigure :loose @21:09:58.73 Forearm.seek :retract @21:09:59.82 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @21:09:59.84 PH.seek 28C,5:00 @21:10:48.53 PH.hold 2:00:00,28C @21:10:48.59 delay 1:30 @21:12:18.62 PSR.select :air @21:12:18.64 PSV.dial PSR @21:12:19.79 Pulling 0.5ml of PSR.wash @21:12:19.80 PSR.select :wash @21:12:19.92 PS.seek 0.5ml @21:12:25.84 delay 5 seconds @21:12:30.85 PSR.select :air @21:12:30.97 PS.seek 2ml @21:12:46.99 PTV.dial :puck @21:12:47.68 PSV.dial PTV @21:12:48.38 PBV.dial :waste1 @21:12:49.66 PS.seek 1.5ml @21:12:55.49 delay 3 seconds @21:12:58.50 PBV.dial between PSV and waste1 @21:12:58.86 PS.seek 0ml @21:13:14.93 delay 5:00 @21:18:14.95 recoverPSRreagent08 @21:18:14.96 PTV.dial :puck @21:18:14.97 PBV.dial :waste1 @21:18:15.32 delay 30 seconds @21:18:45.35 PBV.dial between PSV and waste1 @21:18:45.70 PSR.select :air @21:18:45.71 PSV.dial PSR @21:18:46.44 PS.reconfigure :fast @21:18:46.48 PS.seek 2.5ml @21:19:09.01 PSV.dial PTV @21:19:09.71 delay 5 seconds @21:19:14.73 PSV.dial PSR @21:19:15.45 delay 5 seconds @21:19:20.47 PSV.dial PTV @21:19:21.18 delay 5 seconds @21:19:26.20 PSV.dial :air @21:19:26.92 PS.seek 0ml @21:19:49.32 PTV.dial :PRVmixing,via: PRV @21:19:50.55 PSV.dial :mixloop @21:19:50.99 PRV.dial :ab1 @21:19:51.72 PS.reconfigure :slow @21:19:51.76 PS.seek 0.15ml @21:19:55.26 delay 2 seconds @21:19:57.28 CBV.dial PRV @21:19:57.31
PRV.dial CBV @21:19:57.86 PS.seek 0.34ml @21:20:02.19 delay 2 seconds @21:20:04.21 CBV.dial between PRV and PSV @21:20:04.52 PRV.dial :air @21:20:04.97 PS.seek 0.9ml @21:20:16.79 PRV.dial :waste2 @21:20:17.50 PS.seek 0.5ml @21:20:26.10 addPMLReagent08 @21:20:26.11 PSV.dial :air @21:20:26.56 PS.reconfigure :default @21:20:26.60 PS.seek 2ml @21:20:42.69 PSV.dial :PML @21:20:43.13 PTV.dial :mixing @21:20:43.85 PBV.dial :waste1 @21:20:44.93 PS.seek 1.85ml @21:20:47.38 PBV.dial between PSV and waste1 @21:20:47.77 PS.seek 0ml @21:21:07.38 delay 2 seconds @21:21:09.40 Beginning samplePassC @21:21:09.41 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:21:09.57 Elbow.reconfigure :tight @21:21:09.61 Elbow.seek FlushPuck::Garage @21:21:10.98 Elbow.reconfigure :loose @21:21:11.07 Forearm.seek :garage @21:21:12.21 Hand.close @21:21:12.98 Forearm.seek :retract @21:21:14.01 Elbow.reconfigure :tight @21:21:14.05 Elbow.seek CC @21:21:16.92 Elbow.reconfigure :loose @21:21:17.01 Forearm.reconfigure :tight @21:21:17.05 Forearm.seek CC @21:21:18.86 CC.seek :closed @21:21:23.09 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.80 @21:21:23.18 Hand.open @21:21:23.80 Forearm.reconfigure :loose @21:21:23.84 Forearm.seek :retract @21:21:24.91 PSV.dial PSR @21:21:25.93 Pulling 1.5ml of PSR.flush @21:21:25.94 PSR.select :flush @21:21:26.06 PS.seek 1.5ml @21:21:42.04 delay 5 seconds @21:21:47.06 PSV.dial :waste1 @21:21:47.49 PS.seek 0ml @21:22:03.56 PSV.dial PSR @21:22:03.99 PSR.select :air @21:22:04.10 PS.seek 2.5ml @21:22:30.32 PRV.dial CBV,avoiding: :ab2 @21:22:30.35
CBV.dial PRV @21:22:31.91 PSV.dial :mixloop @21:22:32.90 CTV.dial :air,avoiding: :intake @21:22:32.91 PTV.dial :PRVmixing @21:22:33.62 PS.seek 0ml @21:22:59.84 PSV.dial :air @21:23:00.27 PS.seek 2.5ml @21:23:26.44 PSV.dial :mixloop @21:23:26.88 PS.seek 0ml @21:23:53.00 PSV.dial :air @21:23:53.44 PS.seek 2.5ml @21:24:19.62 PSV.dial :mixloop @21:24:20.07 PS.seek 0ml @21:24:46.25 Garaging FlushPuck in Collection Clamp @21:24:46.32 Elbow.reconfigure :tight @21:24:46.36 Elbow.seek CC @21:24:46.48 Elbow.reconfigure :loose @21:24:46.56 Forearm.seek CC @21:24:47.58 Hand.close @21:24:48.36 CC.seek :open @21:24:52.68 Forearm.seek :retract @21:24:53.67 Elbow.reconfigure :tight @21:24:53.71 Elbow.seek :garage @21:24:56.61 Elbow.reconfigure :loose @21:24:56.70 Forearm.reconfigure :tight @21:24:56.74 Forearm.seek :garage @21:24:57.90 Hand.open @21:24:58.67 Forearm.reconfigure :loose @21:24:58.71 Forearm.seek :retract @21:24:59.80 delayUntil "21:29:09.39" @21:24:59.82 Whole Cell Archive sampling 1000ml @21:24:59.84 Loading wcr puck from tube 3 into Collection Clamp @21:25:00.06 SC.reconfigure :tight @21:25:00.11 SC.seek 3 @21:25:00.22 SC.configure :limp @21:25:00.44 Elbow.reconfigure :tight @21:25:00.48 Elbow.seek 3 @21:25:02.46 Forearm.reconfigure :tight @21:25:02.50 Forearm.seek 3 + 700 counts @21:25:03.56 SE.reconfigure :pick @21:25:03.61 SE.seek :beamBreak @21:25:05.42 SE.configure :default @21:25:05.57 Elbow.seek 3 @21:25:05.74 Forearm.seek 3 + 300 counts @21:25:06.03 Forearm.reconfigure :loose @21:25:06.07 Hand.close @21:25:06.75 SE.seek :down @21:25:06.95 Forearm.seek :retract @21:25:08.43 Elbow.seek CC @21:25:08.52 SE.stop @21:25:09.96 Elbow.reconfigure :loose @21:25:10.06 Forearm.reconfigure :tight @21:25:10.10 Forearm.seek CC @21:25:11.39 CC.seek :closed @21:25:15.65 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.80 @21:25:15.66 Hand.open @21:25:16.42 Forearm.reconfigure :loose @21:25:16.46 Forearm.seek :retract @21:25:17.50 collectSample 1000ml w/bubble point of 35psi @21:25:17.51 Priming 7ml @21:25:17.52 Intake.select :close @21:25:17.53 Exhaust.select :close @21:25:17.54 SS.hold @21:25:17.74 Calibrating pressure sensors @21:25:17.75 CTV.dial :air @21:25:17.77 SSV.dial :spit,avoiding: :exhaust @21:25:17.78 SS.reconfigure :fast @21:25:17.83 SS.seek 5ml @21:25:20.55 SSV.dial CBV @21:25:20.58 CBV.dial SSV @21:25:21.90 delay 3.8 seconds @21:25:25.82 SSV.dial :spit @21:25:27.10 SS.seek 0ml @21:25:29.92 SSV.dial CBV @21:25:31.21 CTV.dial :intake @21:25:31.94 SSV.dial CBV @21:25:31.96 CBV.dial SSV @21:25:31.98 Allowing 40s for 7.0ml of remaining 7.0ml @21:25:31.99 Intake.select :open @21:25:34.14 Intake @15.7psia, vacuum from 10.0 to 13.0psi @21:25:34.16 SS.reconfigure :inhaling 2.14ml/s @21:25:34.20 SS.seek 7ml @21:25:40.80 Stroke completed -- waiting for pressure to equalize ... @21:25:40.81 SS.reconfigure :exhale @21:25:40.85 SS.hold @21:25:50.50 Intake.select :close @21:25:50.65 SSV.dial :exhaust @21:25:51.38 Exhaust.select :open @21:25:51.49 SS.seek 0ml @21:25:55.38 Exhaust.select :close @21:25:55.48 Sampling 1000.0ml @21:25:55.49 Intake.select :close @21:25:55.50 Exhaust.select :close @21:25:55.52 SS.reconfigure :default @21:25:55.55 SS.hold @21:25:55.75 CTV.dial :intake @21:25:55.77 SSV.dial CBV @21:25:55.79 CBV.dial SSV @21:25:56.53 Allowing 130s for 25.0ml of remaining 1000.0ml @21:25:56.55 Intake.select :open @21:25:58.70 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:25:58.72 SS.reconfigure :inhaling 2.14ml/s @21:25:58.76 SS.seek 25ml @21:26:03.13 Vacuum too high -- waiting up to 40 seconds @21:26:03.14 SS.reconfigure :exhale @21:26:03.19 SS.hold @21:26:04.74 Intake @15.3psia, vacuum from 9.8 to 12.8psi @21:26:04.76 SS.reconfigure :inhaling 1.93ml/s @21:26:04.80 SS.seek 25ml @21:26:07.64 Vacuum too high -- waiting up to 40 seconds @21:26:07.65 SS.reconfigure :exhale @21:26:07.69 SS.hold @21:26:10.32 Intake @17.1psia, vacuum from 10.0 to 13.0psi @21:26:10.33 SS.reconfigure :inhaling 1.74ml/s @21:26:10.38 SS.seek 25ml @21:26:12.67 Vacuum too high -- waiting up to 40 seconds @21:26:12.68 SS.reconfigure :exhale @21:26:12.73 SS.hold @21:26:13.82 Intake @17.1psia, vacuum from 10.0 to 13.0psi @21:26:13.84 SS.reconfigure :inhaling 1.57ml/s @21:26:13.88 SS.seek 25ml @21:26:16.10 Vacuum too high -- waiting up to 40 seconds @21:26:16.11 SS.reconfigure :exhale @21:26:16.16 SS.hold @21:26:17.23 Intake @16.9psia, vacuum from 10.0 to 13.0psi @21:26:17.25 SS.reconfigure :inhaling 1.41ml/s @21:26:17.29 SS.seek 25ml @21:26:19.07 Vacuum too high -- waiting up to 40 seconds @21:26:19.08 SS.reconfigure :exhale @21:26:19.12 SS.hold @21:26:20.18 Intake @16.7psia, vacuum from 10.0 to 13.0psi @21:26:20.19 SS.reconfigure :inhaling 1.27ml/s @21:26:20.24 SS.seek 25ml @21:26:22.16 Vacuum too high -- waiting up to 40 seconds @21:26:22.18 SS.reconfigure :exhale @21:26:22.22 SS.hold @21:26:23.23 Intake @16.6psia, vacuum from 10.0 to 13.0psi @21:26:23.25 SS.reconfigure :inhaling 1.14ml/s @21:26:23.29 SS.seek 25ml @21:26:25.45 Vacuum too high -- waiting up to 40 seconds @21:26:25.46 SS.reconfigure :exhale @21:26:25.50 SS.hold @21:26:26.47 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:26:26.48 SS.reconfigure :inhaling 1.03ml/s @21:26:26.53 SS.seek 25ml @21:26:29.85 Vacuum too high -- waiting up to 40 seconds @21:26:29.86 SS.reconfigure :exhale @21:26:29.91 SS.hold @21:26:30.86 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:26:30.88 SS.reconfigure :inhaling 0.93ml/s @21:26:30.92 SS.seek 25ml @21:26:34.23 Vacuum too high -- waiting up to 40 seconds @21:26:34.24 SS.reconfigure :exhale @21:26:34.28 SS.hold @21:26:35.24 Intake @16.5psia, vacuum from 10.0 to 13.0psi @21:26:35.26 SS.reconfigure :inhaling 0.84ml/s @21:26:35.30 SS.seek 25ml @21:26:39.01 Vacuum too high -- waiting up to 40 seconds @21:26:39.02 SS.reconfigure :exhale @21:26:39.06 SS.hold @21:26:40.02 Intake @16.2psia, vacuum from 10.0 to 13.0psi @21:26:40.03 SS.reconfigure :inhaling 0.76ml/s @21:26:40.08 SS.seek 25ml @21:26:41.23 Stroke completed -- waiting for pressure to equalize ... @21:26:41.24 SS.reconfigure :exhale @21:26:41.29 SS.hold @21:26:42.59 Intake.select :close @21:26:42.73 SSV.dial :exhaust @21:26:43.47 Exhaust.select :open @21:26:43.59 SS.seek 0ml @21:26:52.76 Exhaust.select :close @21:26:52.87 SSV.dial CBV @21:26:52.91 CBV.dial SSV @21:26:53.64 Allowing 130s for 25.0ml of remaining 975.0ml @21:26:53.65 Intake.select :open @21:26:55.81 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:26:55.82 SS.reconfigure :inhaling 0.76ml/s @21:26:55.87 SS.seek 25ml @21:27:17.64 Vacuum too high -- waiting up to 40 seconds @21:27:17.65 SS.reconfigure :exhale @21:27:17.69 SS.hold @21:27:18.85 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:27:18.87 SS.reconfigure :inhaling 0.69ml/s @21:27:18.92 SS.seek 25ml @21:27:29.41 Vacuum too high -- waiting up to 40 seconds @21:27:29.42 SS.reconfigure :exhale @21:27:29.47 SS.hold @21:27:30.42 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:27:30.44 SS.reconfigure :inhaling 0.61ml/s @21:27:30.48 SS.seek 25ml @21:27:35.07 Stroke completed -- waiting for pressure to equalize ... @21:27:35.09 SS.reconfigure :exhale @21:27:35.13 SS.hold @21:27:37.08 Intake.select :close @21:27:37.23 SSV.dial :exhaust @21:27:37.95 Exhaust.select :open @21:27:38.06 SS.seek 0ml @21:27:47.43 Exhaust.select :close @21:27:47.54 SSV.dial CBV @21:27:47.57 CBV.dial SSV @21:27:48.33 Allowing 130s for 25.0ml of remaining 950.0ml @21:27:48.34 Intake.select :open @21:27:50.49 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:27:50.51 SS.reconfigure :inhaling 0.61ml/s @21:27:50.56 SS.seek 25ml @21:28:18.94 Vacuum too high -- waiting up to 40 seconds @21:28:18.95 SS.reconfigure :exhale @21:28:19.00 SS.hold @21:28:19.95 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:28:19.97 SS.reconfigure :inhaling 0.56ml/s @21:28:20.01 SS.seek 25ml @21:28:35.56 Stroke completed -- waiting for pressure to equalize ... @21:28:35.57 SS.reconfigure :exhale @21:28:35.61 SS.hold @21:28:39.06 Intake.select :close @21:28:39.20 SSV.dial :exhaust @21:28:39.95 Exhaust.select :open @21:28:40.06 SS.seek 0ml @21:28:49.39 Exhaust.select :close @21:28:49.49 SSV.dial CBV @21:28:49.52 CBV.dial SSV @21:28:50.28 Allowing 130s for 25.0ml of remaining 925.0ml @21:28:50.29 Intake.select :open @21:28:52.44 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:28:52.46 SS.reconfigure :inhaling 0.56ml/s @21:28:52.51 SS.seek 25ml @21:29:09.40 recoverPRVreagent08 @21:29:09.41 PTV.dial :mixing @21:29:10.12 PBV.dial :waste1 @21:29:10.48 delay 30 seconds @21:29:16.97 Vacuum too high -- waiting up to 40 seconds @21:29:16.98 SS.reconfigure :exhale @21:29:17.02 SS.hold @21:29:18.68 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:29:18.69 SS.reconfigure :inhaling 0.50ml/s @21:29:18.74 SS.seek 25ml @21:29:31.88 Vacuum too high -- waiting up to 40 seconds @21:29:31.89 SS.reconfigure :exhale @21:29:31.93 SS.hold @21:29:33.47 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:29:33.49 SS.reconfigure :inhaling 0.46ml/s @21:29:33.53 SS.seek 25ml @21:29:40.50 PBV.dial between PSV and waste1 @21:29:40.85 PSV.dial :air @21:29:41.31 PS.reconfigure :fast @21:29:41.35 PS.seek 2.5ml @21:29:46.37 Vacuum too high -- waiting up to 40 seconds @21:29:46.39 SS.reconfigure :exhale @21:29:46.43 SS.hold @21:29:48.12 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:29:48.14 SS.reconfigure :inhaling 0.41ml/s @21:29:48.18 SS.seek 25ml @21:29:49.09 Stroke completed -- waiting for pressure to equalize ... @21:29:49.10 SS.reconfigure :exhale @21:29:49.14 SS.hold @21:29:50.74 Intake.select :close @21:29:50.88 SSV.dial :exhaust @21:29:51.63 Exhaust.select :open @21:29:51.74 SS.seek 0ml @21:30:00.79 Exhaust.select :close @21:30:00.90 SSV.dial CBV @21:30:00.93 CBV.dial SSV @21:30:01.67 Allowing 130s for 25.0ml of remaining 900.0ml @21:30:01.68 Intake.select :open @21:30:03.83 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:30:03.85 SS.reconfigure :inhaling 0.41ml/s @21:30:03.90 SS.seek 25ml @21:30:03.95 PSV.dial :mixloop @21:30:04.41 delay 2 seconds @21:30:06.43 PSV.dial :air @21:30:06.88 delay 2 seconds @21:30:08.90 PSV.dial :mixloop @21:30:09.46 delay 2 seconds @21:30:11.48 PSV.dial :air @21:30:11.92 PS.seek 0ml @21:30:34.18 washCycleDA #1 of 2 @21:30:34.19 PSR.select :air @21:30:34.20 PSV.dial PSR @21:30:35.51 Pulling 0.5ml of PSR.wash @21:30:35.52 PSR.select :wash @21:30:35.63 PS.reconfigure :default @21:30:35.68 PS.seek 0.5ml @21:30:41.65 delay 5 seconds @21:30:46.67 PSR.select :air @21:30:46.78 PS.seek 2ml @21:31:02.86 PTV.dial :puck @21:31:03.57 PSV.dial PTV @21:31:04.29 PBV.dial :waste1 @21:31:04.71 PS.seek 1.5ml @21:31:07.69 Vacuum too high -- waiting up to 40 seconds @21:31:07.70 SS.reconfigure :exhale @21:31:07.74 SS.hold @21:31:10.66 delay 3 seconds @21:31:12.63 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:31:12.65 SS.reconfigure :inhaling 0.37ml/s @21:31:12.70 SS.seek 25ml @21:31:13.68 PBV.dial between PSV and waste1 @21:31:14.06 PS.seek 0ml @21:31:14.82 Stroke completed -- waiting for pressure to equalize ... @21:31:14.83 SS.reconfigure :exhale @21:31:14.87 SS.hold @21:31:18.94 Intake.select :close @21:31:19.08 SSV.dial :exhaust @21:31:19.83 Exhaust.select :open @21:31:19.95 SS.seek 0ml @21:31:28.88 Exhaust.select :close @21:31:28.99 SSV.dial CBV @21:31:29.01 CBV.dial SSV @21:31:29.74 Allowing 130s for 25.0ml of remaining 875.0ml @21:31:29.76 Intake.select :open @21:31:30.08 delay 2:00 @21:31:31.91 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:31:31.93 SS.reconfigure :inhaling 0.37ml/s @21:31:31.97 SS.seek 25ml @21:32:25.24 Vacuum too high -- waiting up to 40 seconds @21:32:25.26 SS.reconfigure :exhale @21:32:25.30 SS.hold @21:32:31.06 Intake @15.3psia, vacuum from 9.8 to 12.8psi @21:32:31.08 SS.reconfigure :inhaling 0.33ml/s @21:32:31.12 SS.seek 25ml @21:32:57.78 Stroke completed -- waiting for pressure to equalize ... @21:32:57.80 SS.reconfigure :exhale @21:32:57.84 SS.hold @21:33:08.87 Intake.select :close @21:33:09.01 SSV.dial :exhaust @21:33:09.74 Exhaust.select :open @21:33:09.85 SS.seek 0ml @21:33:19.17 Exhaust.select :close @21:33:19.28 SSV.dial CBV @21:33:19.32 CBV.dial SSV @21:33:20.05 Allowing 130s for 25.0ml of remaining 850.0ml @21:33:20.06 Intake.select :open @21:33:22.22 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:33:22.24 SS.reconfigure :inhaling 0.33ml/s @21:33:22.28 SS.seek 25ml @21:33:30.11 recoverPSRreagent08 @21:33:30.12 PTV.dial :puck @21:33:30.13 PBV.dial :waste1 @21:33:30.49 delay 30 seconds @21:33:55.40 Vacuum too high -- waiting up to 40 seconds @21:33:55.41 SS.reconfigure :exhale @21:33:55.45 SS.hold @21:34:00.31 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:34:00.33 SS.reconfigure :inhaling 0.30ml/s @21:34:00.37 SS.seek 25ml @21:34:00.50 PBV.dial between PSV and waste1 @21:34:00.86 PSR.select :air @21:34:00.88 PSV.dial PSR @21:34:01.60 PS.reconfigure :fast @21:34:01.65 PS.seek 2.5ml @21:34:24.20 PSV.dial PTV @21:34:24.92 delay 5 seconds @21:34:29.94 PSV.dial PSR @21:34:30.66 delay 5 seconds @21:34:35.68 PSV.dial PTV @21:34:36.39 delay 5 seconds @21:34:37.49 Vacuum too high -- waiting up to 40 seconds @21:34:37.50 SS.reconfigure :exhale @21:34:37.55 SS.hold @21:34:41.40 PSV.dial :air @21:34:42.13 PS.seek 0ml @21:34:44.12 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:34:44.14 SS.reconfigure :inhaling 0.27ml/s @21:34:44.18 SS.seek 25ml @21:35:04.51 washCycleDA #2 of 2 @21:35:04.52 PSR.select :air @21:35:04.54 PSV.dial PSR @21:35:05.85 Pulling 0.5ml of PSR.wash @21:35:05.86 PSR.select :wash @21:35:05.97 PS.reconfigure :default @21:35:06.01 PS.seek 0.5ml @21:35:09.93 Stroke completed -- waiting for pressure to equalize ... @21:35:09.95 SS.reconfigure :exhale @21:35:09.99 SS.hold @21:35:12.01 delay 5 seconds @21:35:17.03 PSR.select :air @21:35:17.14 PS.seek 2ml @21:35:24.35 Intake.select :close @21:35:24.50 SSV.dial :exhaust @21:35:25.23 Exhaust.select :open @21:35:25.34 SS.seek 0ml @21:35:33.26 PTV.dial :puck @21:35:33.27 PSV.dial PTV @21:35:33.98 PBV.dial :waste1 @21:35:34.41 PS.seek 1.5ml @21:35:34.78 Exhaust.select :close @21:35:34.89 SSV.dial CBV @21:35:34.92 CBV.dial SSV @21:35:35.64 Allowing 130s for 25.0ml of remaining 825.0ml @21:35:35.65 Intake.select :open @21:35:37.80 Intake @15.6psia, vacuum from 10.0 to 13.0psi @21:35:37.82 SS.reconfigure :inhaling 0.27ml/s @21:35:37.87 SS.seek 25ml @21:35:40.29 delay 3 seconds @21:35:43.31 PBV.dial between PSV and waste1 @21:35:43.68 PS.seek 0ml @21:35:59.67 delay 2:00 @21:36:13.89 Vacuum too high -- waiting up to 40 seconds @21:36:13.91 SS.reconfigure :exhale @21:36:13.95 SS.hold @21:36:17.50 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:36:17.52 SS.reconfigure :inhaling 0.24ml/s @21:36:17.56 SS.seek 25ml @21:37:13.08 Vacuum too high -- waiting up to 40 seconds @21:37:13.09 SS.reconfigure :exhale @21:37:13.13 SS.hold @21:37:24.25 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:37:24.27 SS.reconfigure :inhaling 0.21ml/s @21:37:24.31 SS.seek 25ml @21:37:45.68 SS.reconfigure :default @21:37:45.72 SS.hold @21:37:45.88 Intake.select :close @21:37:46.02 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 198.4ml @21:37:46.03 SSV.dial :exhaust @21:37:46.76 Exhaust.select :open @21:37:46.87 SS.reconfigure :exhale @21:37:46.91 SS.seek 0ml @21:37:55.96 Exhaust.select :close @21:37:56.07 Sampled 198.4ml @21:37:56.09 Drying puck before fixing for 20:00 @21:37:56.10 CBV.dial :waste2b @21:37:56.80 delay 1:00 @21:37:59.69 recoverPSRreagent08 @21:37:59.70 PTV.dial :puck @21:37:59.71 PBV.dial :waste1 @21:38:00.06 delay 30 seconds @21:38:30.08 PBV.dial between PSV and waste1 @21:38:30.43 PSR.select :air @21:38:30.45 PSV.dial PSR @21:38:31.17 PS.reconfigure :fast @21:38:31.22 PS.seek 2.5ml @21:38:53.85 PSV.dial PTV @21:38:54.55 delay 5 seconds @21:38:56.82 CSV.dial :air @21:38:57.27 CS.reconfigure :fast @21:38:57.32 CS.seek 10ml @21:38:59.57 PSV.dial PSR @21:39:00.29 delay 5 seconds @21:39:05.31 PSV.dial PTV @21:39:06.02 delay 5 seconds @21:39:11.04 PSV.dial :air @21:39:11.76 PS.seek 0ml @21:39:19.77 Pumping wcr puck from tube 3 up to 35psi... @21:39:20.27 CBV.dial SSV @21:39:21.57 CBV.dial between CSV and waste2b @21:39:22.47 CSV.dial between air and CTV @21:39:22.80 CS.seek 7.332ml @21:39:29.49 CTV.dial CSV,avoiding: :air @21:39:29.52 CSV.dial CTV @21:39:30.29 CS.reconfigure :pressurize @21:39:30.34 CS.seek 0ml @21:39:34.16 getPMLReagent08 @21:39:34.17 PRV.dial :air @21:39:34.62 delay 5 seconds @21:39:39.63 PTV.dial :PRVmixing @21:39:40.87 delay 5 seconds @21:39:43.51 CBV.dial :waste2b @21:39:43.84 CS.reconfigure :default @21:39:43.88 CS.seek 0ml @21:39:45.88 PRV.dial :ab2 @21:39:46.32 PTV.dial :PRVmixing @21:39:46.33 PSV.dial :mixloop @21:39:46.78 PS.reconfigure :default @21:39:46.82 PS.seek 0.3ml @21:39:50.42 delay 1:00 @21:39:50.63 delay 2 seconds @21:39:52.65 PRV.dial :air @21:39:53.16 PS.seek 0.55ml @21:39:56.57 addPMLReagent08 @21:39:56.58 PSV.dial :air @21:39:57.03 PS.seek 2ml @21:40:12.53 PSV.dial :PML @21:40:12.97 PTV.dial :mixing @21:40:13.69 PBV.dial :waste1 @21:40:14.13 PS.seek 1.85ml @21:40:16.53 PBV.dial between PSV and waste1 @21:40:16.90 PS.seek 0ml @21:40:36.44 delay 2 seconds @21:40:38.46 delay 8:00 @21:40:50.44 CTV.dial :air,avoiding: :intake @21:40:51.71 Fix #1 of 2 (with RNAlater) for 10:00 @21:40:51.72 CSV.dial CSR @21:40:52.20 Pulling 2ml of CSR.RNAlater @21:40:52.21 CSR.select :RNAlater @21:40:52.32 CS.seek 2ml @21:40:58.17 delay 5 seconds @21:41:03.19 CSR.select :air @21:41:03.31 CS.reconfigure :fast @21:41:03.35 CS.seek 10ml @21:41:21.49 Pumping wcr puck from tube 3 up to 35psi... @21:41:21.50 CBV.dial SSV @21:41:22.77 CBV.dial between CSV and waste2b @21:41:23.68 CSV.dial between air and CTV @21:41:24.28 CS.seek 7.347ml @21:41:30.86 CTV.dial CSV,avoiding: :air @21:41:30.89 CSV.dial CTV @21:41:32.22 CS.reconfigure :pressurize @21:41:32.27 CS.seek 0ml @21:41:49.38 delay 10:00 @21:48:38.48 recoverPRVreagent08 @21:48:38.49 PTV.dial :mixing @21:48:38.50 PBV.dial :waste1 @21:48:38.85 delay 30 seconds @21:49:08.87 PBV.dial between PSV and waste1 @21:49:09.22 PSV.dial :air @21:49:09.68 PS.reconfigure :fast @21:49:09.73 PS.seek 2.5ml @21:49:32.21 PSV.dial :mixloop @21:49:32.66 delay 2 seconds @21:49:34.68 PSV.dial :air @21:49:35.13 delay 2 seconds @21:49:37.15 PSV.dial :mixloop @21:49:37.59 delay 2 seconds @21:49:39.61 PSV.dial :air @21:49:40.06 PS.seek 0ml @21:50:02.40 washCycleDA #1 of 3 @21:50:02.41 PSR.select :air @21:50:02.42 PSV.dial PSR @21:50:03.73 Pulling 0.5ml of PSR.wash @21:50:03.74 PSR.select :wash @21:50:03.85 PS.reconfigure :default @21:50:03.89 PS.seek 0.5ml @21:50:09.87 delay 5 seconds @21:50:14.88 PSR.select :air @21:50:15.00 PS.seek 2ml @21:50:30.99 PTV.dial :puck @21:50:31.67 PSV.dial PTV @21:50:32.86 PBV.dial :waste1 @21:50:33.28 PS.seek 1.5ml @21:50:39.17 delay 3 seconds @21:50:42.19 PBV.dial between PSV and waste1 @21:50:42.56 PS.seek 0ml @21:50:58.55 delay 2:00 @21:51:49.40 CBV.dial :waste2b @21:51:49.72 CS.reconfigure :default @21:51:49.77 CS.seek 0ml @21:51:52.41 delay 1:00 @21:52:52.43 CTV.dial :air,avoiding: :intake @21:52:53.70 Fix #2 of 2 (with RNAlater) for 10:00 @21:52:53.72 CSV.dial CSR @21:52:54.19 Pulling 2ml of CSR.RNAlater @21:52:54.20 CSR.select :RNAlater @21:52:54.31 CS.seek 2ml @21:52:58.57 recoverPSRreagent08 @21:52:58.58 PTV.dial :puck @21:52:58.59 PBV.dial :waste1 @21:52:58.95 delay 30 seconds @21:53:00.25 delay 5 seconds @21:53:05.27 CSR.select :air @21:53:05.37 CS.reconfigure :fast @21:53:05.42 CS.seek 10ml @21:53:23.62 Pumping wcr puck from tube 3 up to 35psi... @21:53:23.64 CBV.dial SSV @21:53:24.91 CBV.dial between CSV and waste2b @21:53:25.82 CSV.dial between air and CTV @21:53:26.41 CS.seek 7.226ml @21:53:28.97 PBV.dial between PSV and waste1 @21:53:29.33 PSR.select :air @21:53:29.35 PSV.dial PSR @21:53:30.06 PS.reconfigure :fast @21:53:30.11 PS.seek 2.5ml @21:53:33.31 CTV.dial CSV,avoiding: :air @21:53:33.34 CSV.dial CTV @21:53:34.68 CS.reconfigure :pressurize @21:53:34.73 CS.seek 0ml @21:53:51.58 delay 10:00 @21:53:52.64 PSV.dial PTV @21:53:53.36 delay 5 seconds @21:53:58.38 PSV.dial PSR @21:53:59.09 delay 5 seconds @21:54:04.11 PSV.dial PTV @21:54:04.83 delay 5 seconds @21:54:09.84 PSV.dial :air @21:54:10.56 PS.seek 0ml @21:54:32.89 washCycleDA #2 of 3 @21:54:32.90 PSR.select :air @21:54:32.91 PSV.dial PSR @21:54:34.33 Pulling 0.5ml of PSR.wash @21:54:34.34 PSR.select :wash @21:54:34.45 PS.reconfigure :default @21:54:34.49 PS.seek 0.5ml @21:54:40.33 delay 5 seconds @21:54:45.35 PSR.select :air @21:54:45.46 PS.seek 2ml @21:55:01.45 PTV.dial :puck @21:55:01.46 PSV.dial PTV @21:55:02.18 PBV.dial :waste1 @21:55:02.60 PS.seek 1.5ml @21:55:08.51 delay 3 seconds @21:55:11.53 PBV.dial between PSV and waste1 @21:55:11.89 PS.seek 0ml @21:55:27.86 delay 2:00 @21:57:27.88 recoverPSRreagent08 @21:57:27.89 PTV.dial :puck @21:57:27.90 PBV.dial :waste1 @21:57:28.26 delay 30 seconds @21:57:58.28 PBV.dial between PSV and waste1 @21:57:58.63 PSR.select :air @21:57:58.65 PSV.dial PSR @21:57:59.53 PS.reconfigure :fast @21:57:59.57 PS.seek 2.5ml @21:58:22.16 PSV.dial PTV @21:58:22.88 delay 5 seconds @21:58:27.90 PSV.dial PSR @21:58:28.62 delay 5 seconds @21:58:33.63 PSV.dial PTV @21:58:34.35 delay 5 seconds @21:58:39.37 PSV.dial :air @21:58:40.07 PS.seek 0ml @21:59:02.32 washCycleDA #3 of 3 @21:59:02.33 PSR.select :air @21:59:02.34 PSV.dial PSR @21:59:03.65 Pulling 0.5ml of PSR.wash @21:59:03.66 PSR.select :wash @21:59:03.77 PS.reconfigure :default @21:59:03.81 PS.seek 0.5ml @21:59:09.69 delay 5 seconds @21:59:14.71 PSR.select :air @21:59:14.82 PS.seek 2ml @21:59:30.88 PTV.dial :puck @21:59:30.89 PSV.dial PTV @21:59:31.60 PBV.dial :waste1 @21:59:32.01 PS.seek 1.5ml @21:59:38.35 delay 3 seconds @21:59:41.36 PBV.dial between PSV and waste1 @21:59:41.73 PS.seek 0ml @21:59:57.79 delay 2:00 @22:01:57.81 recoverPSRreagent08 @22:01:57.82 PTV.dial :puck @22:01:57.83 PBV.dial :waste1 @22:01:58.19 delay 30 seconds @22:02:28.21 PBV.dial between PSV and waste1 @22:02:28.56 PSR.select :air @22:02:28.58 PSV.dial PSR @22:02:29.30 PS.reconfigure :fast @22:02:29.34 PS.seek 2.5ml @22:02:51.84 PSV.dial PTV @22:02:52.56 delay 5 seconds @22:02:57.58 PSV.dial PSR @22:02:58.30 delay 5 seconds @22:03:03.31 PSV.dial PTV @22:03:04.03 delay 5 seconds @22:03:09.05 PSV.dial :air @22:03:09.77 PS.seek 0ml @22:03:32.18 Gate.power :camera,:ON @22:03:32.20 Mixing DA Substrates PSR.select :air @22:03:32.22 PSV.dial PSR @22:03:33.90 Pulling 0.25ml of PSR.sub1 @22:03:33.95 PSR.select :sub1 @22:03:34.22 PS.reconfigure :default @22:03:34.35 PS.seek 0.25ml @22:03:37.84 delay 5 seconds @22:03:42.91 Pulling 0.25ml of PSR.sub2 @22:03:42.92 PSR.select :sub2 @22:03:43.03 PS.seek 0.5ml @22:03:46.44 delay 5 seconds @22:03:51.45 PSR.select :air @22:03:51.56 PS.seek 2ml @22:03:51.60 CBV.dial :waste2b @22:03:51.94 CS.reconfigure :default @22:03:51.98 CS.seek 0ml @22:03:54.57 delay 1:00 @22:04:07.56 PTV.dial :puck @22:04:07.57 PSV.dial PTV @22:04:08.29 PBV.dial :waste1 @22:04:08.72 PS.seek 1.5ml @22:04:14.56 delay 3 seconds @22:04:17.58 PBV.dial between PSV and waste1 @22:04:17.94 PS.seek 0ml @22:04:33.99 delay 10 seconds @22:04:44.02 recoverPSRreagent08 @22:04:44.03 PTV.dial :puck @22:04:44.04 PBV.dial :waste1 @22:04:44.42 delay 30 seconds @22:04:54.59 CTV.dial :air,avoiding: :intake @22:05:14.44 PBV.dial between PSV and waste1 @22:05:14.79 PSR.select :air @22:05:14.81 PSV.dial PSR @22:05:15.55 PS.reconfigure :fast @22:05:15.59 PS.seek 2.5ml @22:05:38.12 PSV.dial PTV @22:05:38.84 delay 5 seconds @22:05:43.85 PSV.dial PSR @22:05:44.57 delay 5 seconds @22:05:49.59 PSV.dial PTV @22:05:50.31 delay 5 seconds @22:05:55.33 PSV.dial :air @22:05:56.04 PS.seek 0ml @22:06:18.46 Additional air clear @22:06:18.47 PSR.select :air @22:06:18.49 PSV.dial PSR @22:06:19.79 Pulling 2ml of PSR.air @22:06:19.80 PSR.select :air @22:06:19.83 PS.reconfigure :default @22:06:19.87 PS.seek 2ml @22:06:40.99 delay 5 seconds @22:06:46.01 PTV.dial :puck @22:06:46.03 PSV.dial PTV @22:06:46.74 PBV.dial :waste1 @22:06:47.14 PS.seek 1.5ml @22:06:53.02 delay 3 seconds @22:06:56.04 PBV.dial between PSV and waste1 @22:06:56.40 delay 1 second @22:06:57.42 PSV.dial :waste1 @22:06:58.42 PS.seek 0ml @22:07:14.54 Processing Puck Heater at 28C before imaging @22:07:14.55 PH.coast @22:07:14.67 Imaging da puck in Processing Clamp while storing it to tube 2 @22:07:14.83 SC.reconfigure :tight @22:07:14.88 SC.seek 2 @22:07:18.52 SC.configure :limp @22:07:18.71 Elbow.reconfigure :tight @22:07:18.75 Elbow.seek 2 @22:07:20.57 Forearm.reconfigure :tight @22:07:20.61 Forearm.seek 2 + 700 counts @22:07:21.63 SE.reconfigure :pick @22:07:21.68 SE.seek :beamBreak @22:07:23.97 SE.configure :default @22:07:24.11 Elbow.reconfigure :limp @22:07:24.16 Forearm.reconfigure :loose @22:07:24.20 Forearm.seek 2 @22:07:24.60 Elbow.hold @22:07:24.66 Elbow.reconfigure :tight @22:07:25.26 SE.seek 12.27 Pucks below Top Plate @22:07:25.83 Forearm.force -20 @22:07:25.87 Forearm.seek 2 + 315 counts @22:07:26.23 Forearm.reconfigure :tight @22:07:26.27 Forearm.seek 2 + 700 counts @22:07:26.70 SE.seek 13.27 Pucks below Top Plate @22:07:27.31 Forearm.reconfigure :loose @22:07:27.36 Forearm.seek :retract @22:07:28.37 Elbow.seek PC @22:07:31.04 Elbow.reconfigure :loose @22:07:31.13 Forearm.seek PC @22:07:32.21 Hand.close @22:07:32.77 PC.seek :open @22:07:36.81 Forearm.seek :retract @22:07:37.88 Elbow.reconfigure :tight @22:07:37.93 Elbow.seek 2 @22:07:40.55 Forearm.reconfigure :tight @22:07:40.60 Forearm.seek 2 + 700 counts @22:07:41.60 Hand.open @22:07:42.33 SE.seek 12.92 Pucks below Top Plate @22:07:42.61 SE.reconfigure :seal @22:07:42.66 SE.seek 11.81 Pucks below Top Plate @22:07:43.25 Lights, camera, ACTION! @22:07:43.29 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da22dec0922h275ml.tif @22:08:01.20 SX MX5/16 (USB:21): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 14.402 seconds /var/log/esp/da22dec0922h275ml.tif: TIFF Upload Complete @22:08:01.23 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da22dec0922h275ml15s.tif @22:08:02.70 Waiting >20 seconds for da to relinquish FlushPuck @22:08:16.96 SX MX5/16 (USB:21): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da22dec0922h275ml15s.tif: TIFF Upload Complete @22:08:16.99 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da22dec0922h275ml25s.tif @22:08:42.72 SX MX5/16 (USB:21): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da22dec0922h275ml25s.tif: TIFF Upload Complete @22:08:42.74 Gate.power :camera,:OFF @22:08:42.75 DAfans.email "14.402 sec auto-exposure",Subject: "da22dec0922h275ml images!" @22:08:43.05 Elbow.seek 2 @22:08:43.23 Forearm.seek 2 + 700 counts @22:08:43.31 SE.reconfigure :gently @22:08:43.35 SE.seek 12.52 Pucks below Top Plate @22:08:43.98 Elbow.reconfigure :limp @22:08:44.03 Forearm.reconfigure :loose @22:08:44.07 Forearm.seek 2 @22:08:44.47 Elbow.hold @22:08:44.54 Elbow.reconfigure :tight @22:08:44.59 SE.reconfigure :default @22:08:44.64 SE.seek 12.71 Pucks below Top Plate @22:08:45.09 Forearm.force -20 @22:08:45.13 Forearm.seek 2 + 312 counts @22:08:45.50 Forearm.reconfigure :tight @22:08:45.55 Forearm.seek 2 + 700 counts @22:08:45.82 SE.seek :down @22:08:46.95 Forearm.reconfigure :loose @22:08:46.99 Forearm.seek :retract @22:08:47.67 SE.stop @22:08:48.01 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:08:48.27 Elbow.seek FlushPuck::Garage @22:08:50.19 Elbow.reconfigure :loose @22:08:50.37 Forearm.seek :garage @22:08:51.49 Hand.close @22:08:52.27 Forearm.seek :retract @22:08:53.37 Elbow.reconfigure :tight @22:08:53.45 Elbow.seek PC @22:08:54.73 Elbow.reconfigure :loose @22:08:55.36 Forearm.reconfigure :tight @22:08:55.41 Forearm.seek PC @22:08:56.95 PC.seek :closed @22:09:00.93 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.45 @22:09:01.07 Hand.open @22:09:01.91 Forearm.reconfigure :loose @22:09:01.99 Forearm.seek :retract @22:09:03.05 PSR.select :air @22:09:03.11 Storing wcr puck in Collection Clamp to tube 2 @22:09:03.15 PSV.dial PSR @22:09:03.90 Pulling 0.5ml of PSR.flush @22:09:03.93 PSR.select :flush @22:09:04.03 Elbow.reconfigure :tight @22:09:04.08 Elbow.seek 2 @22:09:04.13 PS.seek 0.5ml @22:09:06.83 Forearm.reconfigure :tight @22:09:06.88 Forearm.seek 2 + 700 counts @22:09:08.08 SE.reconfigure :pick @22:09:08.13 SE.seek :beamBreak @22:09:10.36 SE.configure :default @22:09:10.38 delay 5 seconds @22:09:10.54 Elbow.reconfigure :limp @22:09:10.59 Forearm.reconfigure :loose @22:09:10.63 Forearm.seek 2 @22:09:11.03 Elbow.hold @22:09:11.10 Elbow.reconfigure :tight @22:09:11.20 SE.seek 13.27 Pucks below Top Plate @22:09:11.72 Forearm.force -20 @22:09:11.80 Forearm.seek 2 + 311 counts @22:09:12.20 Forearm.reconfigure :tight @22:09:12.24 Forearm.seek 2 + 700 counts @22:09:12.74 SE.seek 14.27 Pucks below Top Plate @22:09:13.28 Forearm.reconfigure :loose @22:09:13.33 Forearm.seek :retract @22:09:14.37 Elbow.seek CC @22:09:15.40 PSR.select :air @22:09:15.51 PS.seek 2.5ml @22:09:15.95 Elbow.reconfigure :loose @22:09:16.04 Forearm.seek CC @22:09:17.02 Hand.close @22:09:17.65 CC.seek :open @22:09:22.10 Forearm.seek :retract @22:09:23.13 Elbow.reconfigure :tight @22:09:23.17 Elbow.seek 2 @22:09:25.00 Elbow.reconfigure :limp @22:09:25.05 Forearm.seek 2 @22:09:26.13 Elbow.hold @22:09:26.20 Elbow.reconfigure :tight @22:09:26.24 Hand.open @22:09:27.13 Forearm.force -20 @22:09:27.17 Forearm.seek 2 + 316 counts @22:09:27.46 Forearm.reconfigure :tight @22:09:27.51 Forearm.seek 2 + 700 counts @22:09:27.96 SE.seek :down @22:09:29.58 Forearm.reconfigure :loose @22:09:29.65 Forearm.seek :retract @22:09:29.72 SE.stop @22:09:30.68
Starting finalPurge @22:09:36.71 delay 1 second @22:09:37.75 PRV.dial :waste2,avoiding: :ab2 @22:09:39.59 PTV.dial :PRVmixing @22:09:40.88 PSV.dial :mixloop @22:09:42.04 PS.seek :empty @22:09:50.53
Waiting >20 seconds for psFlush to relinquish FlushPuck @22:10:08.28 PSR.select :air @22:10:08.39 PSV.dial PSR @22:10:09.52 Pulling 0.5ml of PSR.flush @22:10:09.54 PSR.select :flush @22:10:09.72 PS.seek 0.5ml @22:10:15.72 delay 5 seconds @22:10:20.75 PSR.select :air @22:10:20.91 PS.seek 2.5ml @22:10:42.05 delay 1 second @22:10:43.07 PBV.dial :waste1 @22:10:43.42 PTV.dial :mixing @22:10:44.14 PSV.dial :mixloop @22:10:45.13 PS.seek :empty @22:11:11.33 PSV.dial PSR @22:11:12.31 PSR.select :flush @22:11:12.43 PS.seek 1ml @22:11:23.32 PSR.select :air @22:11:23.46 PS.seek 2.5ml @22:11:39.61 PSV.dial PTV @22:11:40.41 PTV.dial :puck @22:11:41.14 PBV.dial :waste1 @22:11:41.25 PS.seek 0ml @22:12:07.41 PSV.dial PSR @22:12:08.16 PSR.select :flush @22:12:08.32 PS.seek 0.5ml @22:12:14.28 PSR.select :air @22:12:14.46 PS.seek 2.5ml @22:12:35.56 PSV.dial PTV @22:12:36.33 PBV.dial PSV @22:12:36.91 PS.seek 0ml @22:13:00.65 upsync @22:13:03.14 PSV.dial PBV @22:13:03.59 PS.seek 2.5ml @22:13:29.76 PSV.dial :waste1 @22:13:30.47 PS.seek 0ml @22:13:56.63 PSV.dial PSR @22:13:57.07 PSR.select :air @22:13:57.10 PS.seek 2.5ml @22:14:23.29 PSV.dial PTV @22:14:23.99 PBV.dial PSV @22:14:24.01 PS.seek 0ml @22:14:50.18 PSV.dial PBV @22:14:50.65 PS.seek 2.5ml @22:15:16.88 PSV.dial :waste1 @22:15:17.59 PS.seek 0ml @22:15:43.91 PSV.dial PSR @22:15:44.47 PSR.select :flush @22:15:44.59 PS.seek 1ml @22:15:55.66 PSR.select :air @22:15:55.77 PS.seek 2.5ml @22:16:11.75 PSV.dial PTV @22:16:12.47 PTV.dial :puck @22:16:12.49 PBV.dial :waste1 @22:16:13.01 PS.seek 0ml @22:16:39.22 PSV.dial PSR @22:16:39.94 PSR.select :flush @22:16:40.06 PS.seek 0.5ml @22:16:46.00 PSR.select :air @22:16:46.12 PS.seek 2.5ml @22:17:07.25 PSV.dial PTV @22:17:07.97 PBV.dial PSV @22:17:08.51 PS.seek 0ml @22:17:34.69 PSV.dial PBV @22:17:35.15 PS.seek 2.5ml @22:18:01.31 PSV.dial :waste1 @22:18:02.03 PS.seek 0ml @22:18:28.25 PSV.dial PSR @22:18:28.81 PSR.select :air @22:18:28.84 PS.seek 2.5ml @22:18:54.97 PSV.dial PTV @22:18:55.67 PBV.dial PSV @22:18:55.69 PS.seek 0ml @22:19:21.84 PSV.dial PBV @22:19:22.29 PS.seek 2.5ml @22:19:48.75 PSV.dial :waste1 @22:19:49.44 PS.seek 0ml @22:20:15.57
Loading FlushPuck into Collection Clamp from Processing Clamp @22:20:16.20 Elbow.seek PC @22:20:18.79 Elbow.reconfigure :loose @22:20:18.88 Forearm.seek PC @22:20:19.95 Hand.close @22:20:20.50 PC.seek :open @22:20:24.45 Forearm.seek :retract @22:20:25.51 Elbow.reconfigure :tight @22:20:25.57 Elbow.seek CC @22:20:29.28 Elbow.reconfigure :loose @22:20:29.46 Forearm.reconfigure :tight @22:20:29.55 Forearm.seek CC @22:20:31.11 CC.seek :closed @22:20:35.46 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.81 @22:20:35.60 Hand.open @22:20:36.51 Forearm.reconfigure :loose @22:20:36.59 Forearm.seek :retract @22:20:37.69 Flushing the Collection Valve::Manifold @22:20:37.91 CSV.dial CSR @22:20:38.48 Pulling 5ml of CSR.flush CSR.select :flush @22:20:38.66 CS.seek 5ml @22:20:52.08 delay 5 seconds @22:20:57.09 CSR.select :air @22:20:57.32 CS.reconfigure :fast @22:20:57.49 CS.seek 7.5ml @22:21:03.81 CSV.dial :waste @22:21:05.11 CS.reconfigure :default @22:21:05.28 CS.seek 0ml @22:21:25.10 Flushing lower manifold's waste1 port @22:21:25.32 CSV.dial :flush @22:21:25.80 CS.reconfigure :fast @22:21:25.88 CS.seek 5ml @22:21:37.71 delay 5 seconds @22:21:42.76 CSV.dial :air @22:21:43.41 CS.seek 7ml @22:21:48.74 CSV.dial CTV @22:21:48.79
CTV.dial CSV @22:21:50.20 CBV.dial :waste1 @22:21:50.97 CS.seek 0ml @22:22:06.97 Flushing lower manifold's waste2 port @22:22:07.19 CSV.dial :flush @22:22:07.97 CS.seek 5ml @22:22:19.50 delay 5 seconds @22:22:24.54 CSV.dial :air @22:22:25.19 CS.seek 7ml @22:22:30.58 CSV.dial CTV @22:22:30.66
CTV.dial CSV @22:22:31.12 CBV.dial :waste2 @22:22:32.17 CS.seek 0ml @22:22:48.10 Flushing lower manifold's waste2b port @22:22:48.22 CSV.dial :flush @22:22:48.94 CS.seek 5ml @22:23:00.60 delay 5 seconds @22:23:05.62 CSV.dial :air @22:23:06.24 CS.seek 7.001ml @22:23:11.45 CSV.dial CTV @22:23:11.47
CTV.dial CSV @22:23:11.93 CBV.dial :waste2b @22:23:12.92 CS.seek 0ml @22:23:28.66 Disinfecting external Intake with kill @22:23:28.78 CSV.dial CSR @22:23:29.25 Pulling 8ml of CSR.kill @22:23:29.26 CSR.select :kill @22:23:29.37 CS.seek 8ml @22:23:47.48 delay 5 seconds @22:23:52.50 CSR.select :air @22:23:52.61 CS.seek 10ml @22:23:57.87 CSV.dial CBV @22:23:57.93
CBV.dial CSV @22:23:59.01 CTV.dial :intake @22:23:59.79 Intake.select :open @22:23:59.93 CS.seek 2ml @22:24:17.91 Intake.select :close @22:24:18.01 CTV.dial :air @22:24:18.76 CS.reconfigure :default @22:24:18.80 CS.seek 0ml @22:24:24.63 CSV.dial CSR @22:24:25.65 Pulling 5ml of CSR.flush @22:24:25.66 CSR.select :flush @22:24:26.25 CS.seek 5ml @22:24:39.63 delay 5 seconds @22:24:44.65 CSR.select :air @22:24:44.76 CS.seek 10ml @22:24:58.13 CSV.dial CBV @22:24:59.11 CS.seek 0ml @22:25:25.10 Garaging FlushPuck in Collection Clamp @22:25:25.17 Elbow.reconfigure :tight @22:25:25.21 Elbow.seek CC @22:25:25.32 Elbow.reconfigure :loose @22:25:25.41 Forearm.seek CC @22:25:26.42 Hand.close @22:25:26.99 CC.seek :open @22:25:31.33 Forearm.seek :retract @22:25:32.35 Elbow.reconfigure :tight @22:25:32.39 Elbow.seek :garage @22:25:35.34 Elbow.reconfigure :loose @22:25:35.42 Forearm.reconfigure :tight @22:25:35.47 Forearm.seek :garage @22:25:36.62 Hand.open @22:25:37.42 Forearm.reconfigure :loose @22:25:37.46 Forearm.seek :retract @22:25:38.50 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 20:20:24 @22:25:38.55 Asleep after completing DA_WCR_120922 assays @22:25:38.57 GoodNews.email "{1 => 33,\n 2 => 15,\n 3 => 18,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:19:24, 22.5C, 35% humidity, 15.6psia, 14.060V, 0.282A, 0.282A avg, 3.96W\nSampled 198.4ml",Subject: "Asleep after completing DA_WCR_120922 assays" @22:26:13.80
Uploading Logs @22:27:53.03 upsync @22:03:17.75UTC07-Mar-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @22:03:20.32 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @22:03:24.27 Polling Can every 10:00 @22:03:24.36 Cleanly exited at 15:27:58UTC06-Mar-23 @22:03:24.40 Loading /home/esp/esp2/mission/DA_WCR_030623.rb mission @22:03:24.44 Preparing to sleep until + 00:00:05 @22:03:24.46 GoodNews.email "Starting DA_WCR_030623 w/pucks in tube 3\n{1 => 33,\n 2 => 15,\n 3 => 18,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:03:24, 22.5C, 53% humidity, 15.6psia, 14.070V, 0.226A, 0.220A avg, 3.19W",Subject: "DA_WCR_030623 begun" @22:03:24.51
Asleep til + 00:00:05 @22:03:24.55 Continuing at 22:03 @22:03:24.79 Gate.power :core,:OFF @22:03:24.80 Gate.power :raw,:OFF @22:03:24.81 Shutdown @22:03:28.10 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_030623.rb:14\n{1 => 33,\n 2 => 15,\n 3 => 18,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:03:24, 22.5C, 53% humidity, 15.6psia, 14.070V, 0.226A, 0.220A avg, 3.19W",Subject: "Asleep until 22:03" @22:03:29.47
Powering core bus @22:03:31.53 upsync @22:03:34.96
Gate.power :raw,:ON @22:03:35.54 CS.configure :default @22:03:35.63 PS.configure :default @22:03:35.71 SS.configure :default @22:03:35.80 CC.configure :default @22:03:35.88 PC.configure :default @22:03:35.99 Forearm.configure :loose @22:03:36.07 Elbow.configure :loose @22:03:36.18 SC.configure :loose @22:03:36.26 SE.configure :default @22:03:36.30 CH.coast @22:03:36.35 CH.configure :default @22:03:36.38 PH.coast @22:03:36.43 PH.configure :default @22:03:36.46 All dwarf controllers running firmware version 3.85 @22:03:36.57 Valve.reset @22:03:36.78 Intake.select :close @22:03:36.83 Exhaust.select :close @22:03:36.87 CTV.dial :air @22:03:36.94 PSV.dial :air @22:03:37.05 CSV.dial :waste1 @22:03:37.08
Forearm.reconfigure :loose @22:03:37.16 Forearm.home.seek :retract @22:03:37.52 SSV.dial :spit,avoiding: :exhaust @22:03:37.89 Hand.close @22:03:37.92
Elbow.jog 1500 @22:03:38.03 PS.reconfigure :default @22:03:38.07 PS.home.seek 0ml @22:03:38.31 CS.reconfigure :fast @22:03:38.34 SS.reconfigure :default @22:03:38.43 SS.home.seek 0ml @22:03:38.45 CS.home.seek 0ml @22:03:38.57
Elbow.reconfigure :loose @22:03:38.61 Elbow.home.seek :clear @22:03:43.01 SE.reconfigure :default @22:03:43.06 SE.home.seek :down @22:03:43.67 SC.reconfigure :tight @22:03:43.71 SC.home.seek :initial @22:03:46.70 PS.stop @22:04:13.76 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @22:04:13.77
Retry #1 of 3 @22:04:13.83 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @22:04:18.21 SC.configure :limp @22:04:18.26 Forearm.seek :retract @22:04:18.32 Elbow.seek :hammer @22:04:19.44 Forearm.reconfigure :tight @22:04:19.48 Forearm.seek retracted @22:04:19.71 PC.reconfigure :default @22:04:19.74
CC.reconfigure :default @22:04:19.81 PC.home.seek :open @22:04:19.84
CC.home.seek :open @22:04:23.07 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @22:04:23.09 Notice.email "At 22:04:22 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_030623.rb:14\n{1 => 33,\n 2 => 15,\n 3 => 18,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:04:23, 22.6C, 53% humidity, 15.6psia, 14.041V, 0.275A, 0.269A avg, 3.87W",Subject: "Started DA_WCR_030623 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @22:04:23.12
Starting initialPurge @22:04:23.13 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:04:23.19 Hand.open @22:04:24.05 Elbow.reconfigure :tight @22:04:24.09 Elbow.seek FlushPuck::Garage @22:04:25.93 Elbow.reconfigure :loose @22:04:26.02 Forearm.reconfigure :loose @22:04:26.06 Forearm.seek :garage @22:04:27.18 Hand.close @22:04:27.93 Forearm.seek :retract @22:04:28.31 upsync @22:04:29.02
Elbow.reconfigure :tight @22:04:29.06 Elbow.seek CC @22:04:31.93 Elbow.reconfigure :loose @22:04:32.02 Forearm.reconfigure :tight @22:04:32.06 Forearm.seek CC @22:04:33.35 CC.seek :closed @22:04:37.58 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.85 @22:04:37.65 Hand.open @22:04:38.48 Forearm.reconfigure :loose @22:04:38.52 Forearm.seek :retract @22:04:39.55 Flushing the Collection Valve::Manifold @22:04:39.67 CSV.dial CSR @22:04:40.96 Pulling 5ml of CSR.flush @22:04:40.97 CSR.select :flush @22:04:41.08 CS.reconfigure :default @22:04:41.12 CS.seek 5ml @22:04:54.52 delay 5 seconds @22:04:59.54 CSR.select :air @22:04:59.69 CS.reconfigure :fast @22:04:59.74 CS.seek 7.5ml @22:05:06.02 CSV.dial :waste @22:05:07.26 CS.reconfigure :default @22:05:07.30 CS.seek 0ml @22:05:26.99 Purging external Intake @22:05:27.10 CSV.dial :flush @22:05:27.54 CS.reconfigure :fast @22:05:27.58 CS.seek 10ml @22:05:50.05 delay 5 seconds @22:05:55.07 CSV.dial CBV @22:05:55.10
CBV.dial CSV @22:05:55.83 CTV.dial :intake @22:05:56.55 Intake.select :open @22:05:56.65 CS.seek 0ml @22:06:19.05 Intake.select :close @22:06:19.14 CTV.dial :air @22:06:19.88 Flushing upper manifold @22:06:19.99 CSV.dial :flush @22:06:20.71 CS.seek 5ml @22:06:32.36 delay 5 seconds @22:06:37.38 CSV.dial :air @22:06:38.00 CS.seek 7ml @22:06:43.17 CSV.dial CBV @22:06:43.20
CBV.dial CSV @22:06:44.17 CTV.dial :air @22:06:44.19 CS.seek 0ml @22:07:00.05 delay 7 seconds @22:07:07.07 CS.seek 3ml @22:07:14.45 CSV.dial :waste @22:07:14.90 CS.seek 0ml @22:07:22.34 CSV.dial :flush @22:07:22.77 CS.seek 3ml @22:07:30.17 delay 5 seconds @22:07:35.19 CSV.dial :air @22:07:35.69 CS.seek 5ml @22:07:40.86 CSV.dial :air @22:07:40.87 CS.seek 0ml @22:07:52.45 delay 7 seconds @22:07:59.48 CS.seek 3ml @22:08:06.82 CSV.dial :waste @22:08:07.54 CS.seek 0ml @22:08:14.80 Garaging FlushPuck in Collection Clamp @22:08:14.86 Elbow.reconfigure :tight @22:08:14.90 Elbow.seek CC @22:08:15.01 Elbow.reconfigure :loose @22:08:15.10 Forearm.seek CC @22:08:16.07 Hand.close @22:08:16.67 CC.seek :open @22:08:21.00 Forearm.seek :retract @22:08:21.99 Elbow.reconfigure :tight @22:08:22.04 Elbow.seek :garage @22:08:25.12 Elbow.reconfigure :loose @22:08:25.20 Forearm.reconfigure :tight @22:08:25.25 Forearm.seek :garage @22:08:26.39 Hand.open @22:08:27.59 Forearm.reconfigure :loose @22:08:27.70 Forearm.seek :retract @22:08:28.81 da sampling at most 1000ml, wcr at most 1000ml @22:08:28.84 Loading sh1 puck from tube 3 into Collection Clamp @22:08:28.85 DAfans.email "Started da in tube 3 at 22:08:28UTC07-Mar-23\n{1 => 33,\n 2 => 15,\n 3 => 18,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:04:23, 22.6C, 53% humidity, 15.6psia, 14.041V, 0.275A, 0.269A avg, 3.87W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @22:08:29.10
SC.reconfigure :tight @22:08:29.14 SC.seek 3 @22:08:33.72 upsync @22:08:38.96
SC.configure :limp @22:08:39.17 Elbow.reconfigure :tight @22:08:39.22 Elbow.seek 3 @22:08:41.30 Forearm.reconfigure :tight @22:08:41.34 Forearm.seek 3 + 700 counts @22:08:42.38 SE.reconfigure :pick @22:08:42.43 SE.seek :beamBreak @22:08:44.36 SE.configure :default @22:08:44.51 Elbow.seek 3 @22:08:44.75 Forearm.seek 3 + 300 counts @22:08:44.91 Forearm.reconfigure :loose @22:08:44.96 Hand.close @22:08:45.55 SE.seek :down @22:08:45.75
Forearm.seek :retract @22:08:46.68 Elbow.seek CC @22:08:47.17 SE.stop @22:08:48.28
Elbow.reconfigure :loose @22:08:48.37 Forearm.reconfigure :tight @22:08:48.41 Forearm.seek CC @22:08:49.66 CC.seek :closed @22:08:53.88 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.69 @22:08:53.89 Hand.open @22:08:54.73 Forearm.reconfigure :loose @22:08:54.77 Forearm.seek :retract @22:08:55.77 collectSample 1000ml w/bubble point of 24psi @22:08:55.79 Priming 7ml Intake.select :close @22:08:55.80 Exhaust.select :close @22:08:55.81 SS.hold @22:08:56.01 Calibrating pressure and temperature sensors @22:08:56.04 CTV.dial :air @22:08:56.05 SSV.dial :spit,avoiding: :exhaust @22:08:56.07 SS.reconfigure :fast @22:08:56.11 SS.seek 5ml @22:08:59.34 SSV.dial CBV @22:08:59.37
CBV.dial SSV @22:09:00.65 delay 3.8 seconds @22:09:04.56 SSV.dial :spit @22:09:05.84 SS.seek 0ml @22:09:08.32 SSV.dial CBV @22:09:09.60 CTV.dial :intake @22:09:10.31 SSV.dial CBV @22:09:10.33
CBV.dial SSV @22:09:10.34 Allowing 40s for 7.0ml of remaining 7.0ml @22:09:10.35 Intake.select :open @22:09:12.51 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:09:12.52 SS.reconfigure :inhaling 2.14ml/s @22:09:12.57 SS.seek 7ml @22:09:19.44 Stroke completed -- waiting for pressure to equalize ... @22:09:19.45 SS.reconfigure :exhale @22:09:19.49 SS.hold @22:09:22.93 Intake.select :close @22:09:23.07 SSV.dial :exhaust @22:09:23.77 Exhaust.select :open @22:09:23.88 SS.seek 0ml @22:09:27.93 Exhaust.select :close @22:09:28.03 Sampling 1000.0ml @22:09:28.04 Intake.select :close @22:09:28.05 Exhaust.select :close @22:09:28.06 SS.reconfigure :default @22:09:28.10 SS.hold @22:09:28.27 CTV.dial :intake @22:09:28.29 SSV.dial CBV @22:09:28.32
CBV.dial SSV @22:09:29.05 Allowing 130s for 25.0ml of remaining 1000.0ml @22:09:29.06 Intake.select :open @22:09:31.21 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:09:31.23 SS.reconfigure :inhaling 2.14ml/s @22:09:31.27 SS.seek 25ml @22:09:36.50 Vacuum too high -- waiting up to 40 seconds @22:09:36.51 SS.reconfigure :exhale @22:09:36.55 SS.hold @22:09:37.23 Intake @14.0psia, vacuum from 8.5 to 11.5psi @22:09:37.25 SS.reconfigure :inhaling 1.93ml/s @22:09:37.75 SS.seek 25ml @22:09:41.00 Vacuum too high -- waiting up to 40 seconds @22:09:41.01 SS.reconfigure :exhale @22:09:41.05 SS.hold @22:09:41.95 Intake @16.6psia, vacuum from 10.0 to 13.0psi @22:09:41.96 SS.reconfigure :inhaling 1.74ml/s @22:09:42.01 SS.seek 25ml @22:09:44.84 Vacuum too high -- waiting up to 40 seconds @22:09:44.85 SS.reconfigure :exhale @22:09:44.89 SS.hold @22:09:45.75 Intake @18.7psia, vacuum from 10.0 to 13.0psi @22:09:45.77 SS.reconfigure :inhaling 1.57ml/s @22:09:45.81 SS.seek 25ml @22:09:48.19 Vacuum too high -- waiting up to 40 seconds @22:09:48.20 SS.reconfigure :exhale @22:09:48.24 SS.hold @22:09:48.70 Intake @17.8psia, vacuum from 10.0 to 13.0psi @22:09:48.72 SS.reconfigure :inhaling 1.41ml/s @22:09:48.76 SS.seek 25ml @22:09:51.15 Vacuum too high -- waiting up to 40 seconds @22:09:51.16 SS.reconfigure :exhale @22:09:51.21 SS.hold @22:09:51.79 Intake @17.2psia, vacuum from 10.0 to 13.0psi @22:09:51.81 SS.reconfigure :inhaling 1.27ml/s @22:09:51.85 SS.seek 25ml @22:09:54.53 Vacuum too high -- waiting up to 40 seconds @22:09:54.54 SS.reconfigure :exhale @22:09:54.58 SS.hold @22:09:55.05 Intake @18.1psia, vacuum from 10.0 to 13.0psi @22:09:55.06 SS.reconfigure :inhaling 1.14ml/s @22:09:55.11 SS.seek 25ml @22:09:57.25 Vacuum too high -- waiting up to 40 seconds @22:09:57.26 SS.reconfigure :exhale @22:09:57.30 SS.hold @22:09:57.72 Intake @17.5psia, vacuum from 10.0 to 13.0psi @22:09:57.73 SS.reconfigure :inhaling 1.03ml/s @22:09:57.78 SS.seek 25ml @22:10:04.71 Stroke completed -- waiting for pressure to equalize ... @22:10:04.72 SS.reconfigure :exhale @22:10:04.76 SS.hold @22:10:06.30 Intake.select :close @22:10:06.44 SSV.dial :exhaust @22:10:07.16 Exhaust.select :open @22:10:07.27 SS.seek 0ml @22:10:16.05 Exhaust.select :close @22:10:16.15 SSV.dial CBV @22:10:16.18
CBV.dial SSV @22:10:16.93 Allowing 130s for 25.0ml of remaining 975.0ml @22:10:16.94 Intake.select :open @22:10:19.09 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:10:19.10 SS.reconfigure :inhaling 1.03ml/s @22:10:19.14 SS.seek 25ml @22:10:46.27 Stroke completed -- waiting for pressure to equalize ... @22:10:46.28 SS.reconfigure :exhale @22:10:46.32 SS.hold @22:10:47.86 Intake.select :close @22:10:48.00 SSV.dial :exhaust @22:10:48.72 Exhaust.select :open @22:10:48.83 SS.seek 0ml @22:10:57.56 Exhaust.select :close @22:10:57.67 SSV.dial CBV @22:10:57.69
CBV.dial SSV @22:10:58.44 Allowing 130s for 25.0ml of remaining 950.0ml @22:10:58.45 Intake.select :open @22:11:00.60 Intake @15.5psia, vacuum from 10.0 to 13.0psi @22:11:00.62 SS.reconfigure :inhaling 1.03ml/s @22:11:00.66 SS.seek 25ml @22:11:27.66 Stroke completed -- waiting for pressure to equalize ... @22:11:27.67 SS.reconfigure :exhale @22:11:27.71 SS.hold @22:11:29.65 Intake.select :close @22:11:29.79 SSV.dial :exhaust @22:11:30.52 Exhaust.select :open @22:11:30.62 SS.seek 0ml @22:11:39.42 Exhaust.select :close @22:11:39.52 SSV.dial CBV @22:11:39.55
CBV.dial SSV @22:11:40.30 Allowing 130s for 25.0ml of remaining 925.0ml @22:11:40.31 Intake.select :open @22:11:42.46 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:11:42.47 SS.reconfigure :inhaling 1.03ml/s @22:11:42.51 SS.seek 25ml @22:12:09.47 Stroke completed -- waiting for pressure to equalize ... @22:12:09.48 SS.reconfigure :exhale @22:12:09.52 SS.hold @22:12:11.46 Intake.select :close @22:12:11.59 SSV.dial :exhaust @22:12:12.31 Exhaust.select :open @22:12:12.42 SS.seek 0ml @22:12:21.22 Exhaust.select :close @22:12:21.32 SSV.dial CBV @22:12:21.36
CBV.dial SSV @22:12:22.06 Allowing 130s for 25.0ml of remaining 900.0ml @22:12:22.07 Intake.select :open @22:12:24.22 Intake @15.9psia, vacuum from 10.0 to 13.0psi @22:12:24.24 SS.reconfigure :inhaling 1.03ml/s @22:12:24.28 SS.seek 25ml @22:12:51.17 Stroke completed -- waiting for pressure to equalize ... @22:12:51.18 SS.reconfigure :exhale @22:12:51.22 SS.hold @22:12:53.16 Intake.select :close @22:12:53.30 SSV.dial :exhaust @22:12:54.03 Exhaust.select :open @22:12:54.14 SS.seek 0ml @22:13:02.92 Exhaust.select :close @22:13:03.02 SSV.dial CBV @22:13:03.05
CBV.dial SSV @22:13:03.80 Allowing 130s for 25.0ml of remaining 875.0ml @22:13:03.81 Intake.select :open @22:13:05.96 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:13:05.97 SS.reconfigure :inhaling 1.03ml/s @22:13:06.02 SS.seek 25ml @22:13:32.75 Stroke completed -- waiting for pressure to equalize ... @22:13:32.76 SS.reconfigure :exhale @22:13:32.80 SS.hold @22:13:34.69 Intake.select :close @22:13:34.83 SSV.dial :exhaust @22:13:35.55 Exhaust.select :open @22:13:35.65 SS.seek 0ml @22:13:44.57 Exhaust.select :close @22:13:44.68 SSV.dial CBV @22:13:44.71
CBV.dial SSV @22:13:45.44 Allowing 130s for 25.0ml of remaining 850.0ml @22:13:45.45 Intake.select :open @22:13:47.60 Intake @15.3psia, vacuum from 9.8 to 12.8psi @22:13:47.61 SS.reconfigure :inhaling 1.03ml/s @22:13:47.66 SS.seek 25ml @22:14:13.81 Stroke completed -- waiting for pressure to equalize ... @22:14:13.82 SS.reconfigure :exhale @22:14:13.86 SS.hold @22:14:15.39 Intake.select :close @22:14:15.53 SSV.dial :exhaust @22:14:16.26 Exhaust.select :open @22:14:16.37 SS.seek 0ml @22:14:25.31 Exhaust.select :close @22:14:25.41 SSV.dial CBV @22:14:25.46
CBV.dial SSV @22:14:26.17 Allowing 130s for 25.0ml of remaining 825.0ml @22:14:26.18 Intake.select :open @22:14:28.33 Intake @15.2psia, vacuum from 9.7 to 12.7psi @22:14:28.35 SS.reconfigure :inhaling 1.03ml/s @22:14:28.39 SS.seek 25ml @22:14:55.15 Stroke completed -- waiting for pressure to equalize ... @22:14:55.16 SS.reconfigure :exhale @22:14:55.20 SS.hold @22:14:57.15 Intake.select :close @22:14:57.28 SSV.dial :exhaust @22:14:58.01 Exhaust.select :open @22:14:58.12 SS.seek 0ml @22:15:07.03 Exhaust.select :close @22:15:07.13 SSV.dial CBV @22:15:07.17
CBV.dial SSV @22:15:07.89 Allowing 130s for 25.0ml of remaining 800.0ml @22:15:07.90 Intake.select :open @22:15:10.05 Intake @15.4psia, vacuum from 9.9 to 12.9psi @22:15:10.06 SS.reconfigure :inhaling 1.03ml/s @22:15:10.10 SS.seek 25ml @22:15:36.98 Stroke completed -- waiting for pressure to equalize ... @22:15:36.99 SS.reconfigure :exhale @22:15:37.03 SS.hold @22:15:38.66 Intake.select :close @22:15:38.79 SSV.dial :exhaust @22:15:39.53 Exhaust.select :open @22:15:39.63 SS.seek 0ml @22:15:48.55 Exhaust.select :close @22:15:48.66 SSV.dial CBV @22:15:48.68
CBV.dial SSV @22:15:49.42 Allowing 130s for 25.0ml of remaining 775.0ml @22:15:49.43 Intake.select :open @22:15:51.58 Intake @15.3psia, vacuum from 9.8 to 12.8psi @22:15:51.59 SS.reconfigure :inhaling 1.03ml/s @22:15:51.64 SS.seek 25ml @22:16:18.05 Vacuum too high -- waiting up to 40 seconds @22:16:18.06 SS.reconfigure :exhale @22:16:18.10 SS.hold @22:16:18.48 Intake @12.5psia, vacuum from 7.0 to 10.0psi @22:16:18.49 SS.reconfigure :inhaling 0.93ml/s @22:16:18.53 SS.seek 25ml @22:16:18.97 Stroke completed -- waiting for pressure to equalize ... @22:16:18.98 SS.reconfigure :exhale @22:16:19.02 SS.hold @22:16:20.97 Intake.select :close @22:16:21.11 SSV.dial :exhaust @22:16:21.84 Exhaust.select :open @22:16:21.94 SS.seek 0ml @22:16:31.23 Exhaust.select :close @22:16:31.33 SSV.dial CBV @22:16:31.35
CBV.dial SSV @22:16:32.10 Allowing 130s for 25.0ml of remaining 750.0ml @22:16:32.11 Intake.select :open @22:16:34.26 Intake @15.2psia, vacuum from 9.7 to 12.7psi @22:16:34.28 SS.reconfigure :inhaling 0.93ml/s @22:16:34.32 SS.seek 25ml @22:17:02.94 Stroke completed -- waiting for pressure to equalize ... @22:17:02.95 SS.reconfigure :exhale @22:17:02.99 SS.hold @22:17:04.93 Intake.select :close @22:17:05.08 SSV.dial :exhaust @22:17:05.80 Exhaust.select :open @22:17:05.91 SS.seek 0ml @22:17:14.83 Exhaust.select :close @22:17:14.93 SSV.dial CBV @22:17:14.96
CBV.dial SSV @22:17:15.69 Allowing 130s for 25.0ml of remaining 725.0ml @22:17:15.70 Intake.select :open @22:17:17.85 Intake @15.2psia, vacuum from 9.7 to 12.7psi @22:17:17.87 SS.reconfigure :inhaling 0.93ml/s @22:17:17.91 SS.seek 25ml @22:17:47.33 Stroke completed -- waiting for pressure to equalize ... @22:17:47.34 SS.reconfigure :exhale @22:17:47.38 SS.hold @22:17:50.27 Intake.select :close @22:17:50.40 SSV.dial :exhaust @22:17:51.13 Exhaust.select :open @22:17:51.24 SS.seek 0ml @22:18:00.21 Exhaust.select :close @22:18:00.31 SSV.dial CBV @22:18:00.34
CBV.dial SSV @22:18:01.08 Allowing 130s for 25.0ml of remaining 700.0ml @22:18:01.10 Intake.select :open @22:18:03.25 Intake @15.2psia, vacuum from 9.7 to 12.7psi @22:18:03.26 SS.reconfigure :inhaling 0.93ml/s @22:18:03.31 SS.seek 25ml @22:18:22.52 Vacuum too high -- waiting up to 40 seconds @22:18:22.53 SS.reconfigure :exhale @22:18:22.57 SS.hold @22:18:23.70 Intake @13.2psia, vacuum from 7.7 to 10.7psi @22:18:23.72 SS.reconfigure :inhaling 0.84ml/s @22:18:23.76 SS.seek 25ml @22:18:35.47 Stroke completed -- waiting for pressure to equalize ... @22:18:35.48 SS.reconfigure :exhale @22:18:35.52 SS.hold @22:18:38.41 Intake.select :close @22:18:38.55 SSV.dial :exhaust @22:18:39.27 Exhaust.select :open @22:18:39.37 SS.seek 0ml @22:18:48.33 Exhaust.select :close @22:18:48.43 SSV.dial CBV @22:18:48.46
CBV.dial SSV @22:18:49.19 Allowing 130s for 25.0ml of remaining 675.0ml @22:18:49.20 Intake.select :open @22:18:51.35 Intake @15.2psia, vacuum from 9.7 to 12.7psi @22:18:51.37 SS.reconfigure :inhaling 0.84ml/s @22:18:51.41 SS.seek 25ml @22:19:14.13 Vacuum too high -- waiting up to 40 seconds @22:19:14.15 SS.reconfigure :exhale @22:19:14.19 SS.hold @22:19:14.57 Intake @12.9psia, vacuum from 7.4 to 10.4psi @22:19:14.59 SS.reconfigure :inhaling 0.76ml/s @22:19:14.63 SS.seek 25ml @22:19:25.59 Stroke completed -- waiting for pressure to equalize ... @22:19:25.60 SS.reconfigure :exhale @22:19:25.64 SS.hold @22:19:28.54 Intake.select :close @22:19:28.68 SSV.dial :exhaust @22:19:29.41 Exhaust.select :open @22:19:29.51 SS.seek 0ml @22:19:38.86 Exhaust.select :close @22:19:38.96 SSV.dial CBV @22:19:38.99
CBV.dial SSV @22:19:39.74 Allowing 130s for 25.0ml of remaining 650.0ml @22:19:39.75 Intake.select :open @22:19:41.90 Intake @15.2psia, vacuum from 9.7 to 12.7psi @22:19:41.91 SS.reconfigure :inhaling 0.76ml/s @22:19:41.96 SS.seek 25ml @22:20:09.94 Vacuum too high -- waiting up to 40 seconds @22:20:09.95 SS.reconfigure :exhale @22:20:10.00 SS.hold @22:20:10.96 Intake @13.5psia, vacuum from 8.0 to 11.0psi @22:20:10.97 SS.reconfigure :inhaling 0.69ml/s @22:20:11.01 SS.seek 25ml @22:20:18.69 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @22:20:18.73 SS.hold @22:20:21.63 Intake.select :close @22:20:21.77 SSV.dial :exhaust @22:20:22.48 Exhaust.select :open @22:20:22.59 SS.seek 0ml @22:20:31.58 Exhaust.select :close @22:20:31.68 SSV.dial CBV @22:20:31.73
CBV.dial SSV @22:20:32.42 Allowing 130s for 25.0ml of remaining 625.0ml @22:20:32.43 Intake.select :open @22:20:34.58 Intake @15.2psia, vacuum from 9.7 to 12.7psi @22:20:34.60 SS.reconfigure :inhaling 0.69ml/s @22:20:34.64 SS.seek 25ml @22:21:04.22 Vacuum too high -- waiting up to 40 seconds @22:21:04.23 SS.reconfigure :exhale @22:21:04.27 SS.hold @22:21:05.55 Intake @13.6psia, vacuum from 8.1 to 11.1psi @22:21:05.56 SS.reconfigure :inhaling 0.61ml/s @22:21:05.61 SS.seek 25ml @22:21:16.62 Stroke completed -- waiting for pressure to equalize ... @22:21:16.63 SS.reconfigure :exhale @22:21:16.67 SS.hold @22:21:20.11 Intake.select :close @22:21:20.25 SSV.dial :exhaust @22:21:20.97 Exhaust.select :open @22:21:21.07 SS.seek 0ml @22:21:30.40 Exhaust.select :close @22:21:30.50 SSV.dial CBV @22:21:30.53
CBV.dial SSV @22:21:31.28 Allowing 130s for 25.0ml of remaining 600.0ml @22:21:31.29 Intake.select :open @22:21:33.44 Intake @15.3psia, vacuum from 9.8 to 12.8psi @22:21:33.46 SS.reconfigure :inhaling 0.61ml/s @22:21:33.50 SS.seek 25ml @22:22:06.85 Vacuum too high -- waiting up to 40 seconds @22:22:06.86 SS.reconfigure :exhale @22:22:06.90 SS.hold @22:22:08.91 Intake @13.9psia, vacuum from 8.4 to 11.4psi @22:22:08.92 SS.reconfigure :inhaling 0.56ml/s @22:22:08.96 SS.seek 25ml @22:22:19.62 Stroke completed -- waiting for pressure to equalize ... @22:22:19.63 SS.reconfigure :exhale @22:22:19.67 SS.hold @22:22:23.75 Intake.select :close @22:22:23.88 SSV.dial :exhaust @22:22:24.61 Exhaust.select :open @22:22:24.72 SS.seek 0ml @22:22:34.13 Exhaust.select :close @22:22:34.24 SSV.dial CBV @22:22:34.27
CBV.dial SSV @22:22:35.00 Allowing 130s for 25.0ml of remaining 575.0ml @22:22:35.01 Intake.select :open @22:22:37.16 Intake @15.3psia, vacuum from 9.8 to 12.8psi @22:22:37.17 SS.reconfigure :inhaling 0.56ml/s @22:22:37.22 SS.seek 25ml @22:23:23.47 Vacuum too high -- waiting up to 40 seconds @22:23:23.48 SS.reconfigure :exhale @22:23:23.52 SS.hold @22:23:26.01 Intake @14.4psia, vacuum from 8.9 to 11.9psi @22:23:26.02 SS.reconfigure :inhaling 0.50ml/s @22:23:26.07 SS.seek 25ml @22:23:27.48 Stroke completed -- waiting for pressure to equalize ... @22:23:27.49 SS.reconfigure :exhale @22:23:27.53 SS.hold @22:23:30.42 Intake.select :close @22:23:30.55 SSV.dial :exhaust @22:23:31.27 Exhaust.select :open @22:23:31.38 SS.seek 0ml @22:23:40.34 Exhaust.select :close @22:23:40.44 SSV.dial CBV @22:23:40.47
CBV.dial SSV @22:23:41.21 Allowing 130s for 25.0ml of remaining 550.0ml @22:23:41.22 Intake.select :open @22:23:43.37 Intake @15.4psia, vacuum from 9.9 to 12.9psi @22:23:43.39 SS.reconfigure :inhaling 0.50ml/s @22:23:43.43 SS.seek 25ml @22:24:37.58 Stroke completed -- waiting for pressure to equalize ... @22:24:37.59 SS.reconfigure :exhale @22:24:37.63 SS.hold @22:24:44.97 Intake.select :close @22:24:45.11 SSV.dial :exhaust @22:24:45.83 Exhaust.select :open @22:24:45.94 SS.seek 0ml @22:24:55.32 Exhaust.select :close @22:24:55.42 SSV.dial CBV @22:24:55.47
CBV.dial SSV @22:24:56.16 Allowing 130s for 25.0ml of remaining 525.0ml @22:24:56.17 Intake.select :open @22:24:58.32 Intake @15.4psia, vacuum from 9.9 to 12.9psi @22:24:58.34 SS.reconfigure :inhaling 0.50ml/s @22:24:58.38 SS.seek 25ml @22:25:31.00 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @22:25:31.04 SS.hold @22:25:33.92 Intake @14.6psia, vacuum from 9.1 to 12.1psi @22:25:33.93 SS.reconfigure :inhaling 0.46ml/s @22:25:33.98 SS.seek 25ml @22:25:57.46 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @22:25:57.50 SS.hold @22:26:05.35 Intake.select :close @22:26:05.49 SSV.dial :exhaust @22:26:06.21 Exhaust.select :open @22:26:06.31 SS.seek 0ml @22:26:15.32 Exhaust.select :close @22:26:15.42 SSV.dial CBV @22:26:15.45
CBV.dial SSV @22:26:16.19 Allowing 130s for 25.0ml of remaining 500.0ml @22:26:16.20 Intake.select :open @22:26:18.35 Intake @15.5psia, vacuum from 10.0 to 13.0psi @22:26:18.37 SS.reconfigure :inhaling 0.46ml/s @22:26:18.41 SS.seek 25ml @22:27:00.90 Vacuum too high -- waiting up to 40 seconds @22:27:00.91 SS.reconfigure :exhale @22:27:00.95 SS.hold @22:27:05.10 Intake @15.4psia, vacuum from 9.9 to 12.9psi @22:27:05.12 SS.reconfigure :inhaling 0.41ml/s @22:27:05.16 SS.seek 25ml @22:27:26.34 Stroke completed -- waiting for pressure to equalize ... @22:27:26.35 SS.reconfigure :exhale @22:27:26.39 SS.hold @22:27:34.83 Intake.select :close @22:27:34.97 SSV.dial :exhaust @22:27:35.69 Exhaust.select :open @22:27:35.79 SS.seek 0ml @22:27:44.83 Exhaust.select :close @22:27:44.93 SSV.dial CBV @22:27:44.98
CBV.dial SSV @22:27:45.67 Allowing 130s for 25.0ml of remaining 475.0ml @22:27:45.68 Intake.select :open @22:27:47.83 Intake @15.5psia, vacuum from 10.0 to 13.0psi @22:27:47.85 SS.reconfigure :inhaling 0.41ml/s @22:27:47.89 SS.seek 25ml @22:28:39.27 Vacuum too high -- waiting up to 40 seconds @22:28:39.28 SS.reconfigure :exhale @22:28:39.32 SS.hold @22:28:44.59 SS.reconfigure :inhaling 0.37ml/s @22:28:44.63 SS.seek 25ml @22:29:06.39 Stroke completed -- waiting for pressure to equalize ... @22:29:06.40 SS.reconfigure :exhale @22:29:06.44 SS.hold @22:29:14.87 Intake.select :close @22:29:15.01 SSV.dial :exhaust @22:29:15.73 Exhaust.select :open @22:29:15.84 SS.seek 0ml @22:29:24.87 Exhaust.select :close @22:29:24.97 SSV.dial CBV @22:29:25.00
CBV.dial SSV @22:29:25.75 Allowing 130s for 25.0ml of remaining 450.0ml @22:29:25.76 Intake.select :open @22:29:27.91 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:29:27.92 SS.reconfigure :inhaling 0.37ml/s @22:29:27.97 SS.seek 25ml @22:30:48.82 Stroke completed -- waiting for pressure to equalize ... @22:30:48.83 SS.reconfigure :exhale @22:30:48.87 SS.hold @22:31:03.58 Intake.select :close @22:31:03.72 SSV.dial :exhaust @22:31:04.45 Exhaust.select :open @22:31:04.55 SS.seek 0ml @22:31:14.06 Exhaust.select :close @22:31:14.16 SSV.dial CBV @22:31:14.19
CBV.dial SSV @22:31:14.92 Allowing 130s for 25.0ml of remaining 425.0ml @22:31:14.93 Intake.select :open @22:31:17.08 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:31:17.09 SS.reconfigure :inhaling 0.37ml/s @22:31:17.14 SS.seek 25ml @22:32:10.02 Vacuum too high -- waiting up to 40 seconds @22:32:10.03 SS.reconfigure :exhale @22:32:10.07 SS.hold @22:32:17.41 Intake @15.6psia, vacuum from 10.0 to 13.0psi @22:32:17.43 SS.reconfigure :inhaling 0.33ml/s @22:32:17.47 SS.seek 25ml @22:32:49.77 Stroke completed -- waiting for pressure to equalize ... @22:32:49.78 SS.reconfigure :exhale @22:32:49.82 SS.hold @22:33:04.06 Intake.select :close @22:33:04.19 SSV.dial :exhaust @22:33:04.91 Exhaust.select :open @22:33:05.02 SS.seek 0ml @22:33:14.49 Exhaust.select :close @22:33:14.59 SSV.dial CBV @22:33:14.62
CBV.dial SSV @22:33:15.37 Allowing 130s for 25.0ml of remaining 400.0ml @22:33:15.38 Intake.select :open @22:33:17.53 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:33:17.54 SS.reconfigure :inhaling 0.33ml/s @22:33:17.59 SS.seek 25ml @22:34:17.01 Vacuum too high -- waiting up to 40 seconds @22:34:17.03 SS.reconfigure :exhale @22:34:17.07 SS.hold @22:34:24.44 Intake @15.7psia, vacuum from 10.0 to 13.0psi @22:34:24.46 SS.reconfigure :inhaling 0.30ml/s @22:34:24.50 SS.seek 25ml @22:35:00.84 Stroke completed -- waiting for pressure to equalize ... @22:35:00.85 SS.reconfigure :exhale @22:35:00.89 SS.hold @22:35:17.55 Intake.select :close @22:35:17.68 SSV.dial :exhaust @22:35:18.41 Exhaust.select :open @22:35:18.51 SS.seek 0ml @22:35:27.59 Exhaust.select :close @22:35:27.70 SSV.dial CBV @22:35:27.72
CBV.dial SSV @22:35:28.46 Allowing 130s for 25.0ml of remaining 375.0ml @22:35:28.47 Intake.select :open @22:35:30.62 Intake @15.8psia, vacuum from 10.0 to 13.0psi @22:35:30.63 SS.reconfigure :inhaling 0.30ml/s @22:35:30.68 SS.seek 25ml @22:36:56.16 Vacuum too high -- waiting up to 40 seconds @22:36:56.17 SS.reconfigure :exhale @22:36:56.21 SS.hold @22:37:08.84 Intake @16.1psia, vacuum from 10.0 to 13.0psi @22:37:08.85 SS.reconfigure :inhaling 0.27ml/s @22:37:08.89 SS.seek 25ml @22:37:28.71 Stroke completed -- waiting for pressure to equalize ... @22:37:28.72 SS.reconfigure :exhale @22:37:28.76 SS.hold @22:37:38.48 SS.reconfigure :default @22:37:38.51 SS.hold @22:37:38.62 Intake.select :close @22:37:38.75 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 650.0ml @22:37:38.76 SSV.dial :exhaust @22:37:39.50 Exhaust.select :open @22:37:39.60 SS.reconfigure :exhale @22:37:39.64 SS.seek 0ml @22:37:49.15 Exhaust.select :close @22:37:49.25 Sampled 650.0ml @22:37:49.27 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @22:37:49.33 CSV.dial :waste @22:37:49.37 CS.seek 0ml @22:37:49.46 SSV.dial CBV,avoiding: :exhaust @22:37:49.49
CBV.dial SSV @22:37:49.55 CSV.dial :air @22:37:50.27 CS.seek 10ml @22:37:51.31
CTV.dial :air @22:37:52.05 delay 15 seconds @22:38:07.06 SSV.dial :spit,avoiding: :exhaust @22:38:08.36 CTV.dial CSV,avoiding: :intake @22:38:08.39
SS.seek 0ml @22:38:08.51 SSV.dial CBV,avoiding: :exhaust @22:38:12.92 CBV.dial between SSV and waste2 @22:38:13.31 CTV.dial CSV,avoiding: :air @22:38:13.33
CSV.dial CTV @22:38:13.77 CS.reconfigure :pressurize @22:38:13.81 CS.seek 0ml @22:38:32.81 BubblePoint: Intake @40.5psia, Outlet @16.3psia @22:38:32.82 CBV.dial SSV @22:38:33.14 SS.reconfigure :default @22:38:33.18 SS.seek 10ml @22:38:38.59 delay 15 seconds @22:38:53.70 Evacuated: Intake @16.3psia, Outlet @11.2psia @22:38:53.71 SSV.dial :spit,avoiding: :exhaust @22:38:55.02 SS.reconfigure :exhale @22:38:55.06 SS.seek 0ml @22:39:00.55 SS.reconfigure :fast @22:39:00.59 SS.seek 5ml @22:39:03.62 SSV.dial CBV,avoiding: :exhaust @22:39:03.65
CBV.dial SSV @22:39:04.94 CTV.dial :air,avoiding: :intake @22:39:06.20 CSV.dial CTV @22:39:06.23
SS.seek 10ml @22:39:07.22 CSV.dial :air @22:39:07.78 CS.reconfigure :fast @22:39:07.82 CS.seek 0ml @22:39:08.92
SSV.dial :spit,avoiding: :exhaust @22:39:10.68 SS.reconfigure :exhale @22:39:10.72 SS.seek 0ml @22:39:14.52 CS.stop @22:39:16.41
SS.reconfigure :fast @22:39:16.45 SS.seek 5ml @22:39:19.03 SSV.dial CBV,avoiding: :exhaust @22:39:19.06
CBV.dial SSV @22:39:21.35 SSV.dial :spit,avoiding: :exhaust @22:39:22.64 SS.reconfigure :default @22:39:22.68 SS.seek 0ml @22:39:26.06 SS.stop @22:39:26.09 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @22:39:26.10 Intake.select :close @22:39:26.17 CSV.dial :waste @22:39:26.28 SSV.dial CBV,avoiding: :exhaust @22:39:26.31
CBV.dial SSV @22:39:26.94 CS.seek 0ml @22:39:27.00 CSV.dial :air @22:39:27.61
CTV.dial :air @22:39:27.62 delay 15 seconds @22:39:27.72 CS.seek 10ml @22:39:42.64
SSV.dial :spit,avoiding: :exhaust @22:39:43.94 CTV.dial CSV,avoiding: :intake @22:39:43.97
SS.reconfigure :exhale @22:39:44.06 SS.seek 0ml @22:39:44.12 SSV.dial CBV,avoiding: :exhaust @22:39:50.17 CBV.dial between SSV and waste2 @22:39:50.55 CTV.dial CSV,avoiding: :air @22:39:50.58
CSV.dial CTV @22:39:51.01 CS.reconfigure :pressurize @22:39:51.06 CS.seek 0ml @22:40:12.37 BubblePoint: Intake @39.8psia, Outlet @15.4psia @22:40:12.38 CBV.dial SSV @22:40:12.70 SS.reconfigure :default @22:40:12.74 SS.seek 10ml @22:40:18.23 delay 15 seconds @22:40:33.34 Evacuated: Intake @19.0psia, Outlet @12.4psia @22:40:33.35 SSV.dial :spit,avoiding: :exhaust @22:40:34.64 SS.reconfigure :exhale @22:40:34.68 SS.seek 0ml @22:40:40.11 SS.reconfigure :fast @22:40:40.15 SS.seek 5ml @22:40:43.14 SSV.dial CBV,avoiding: :exhaust @22:40:43.16
CBV.dial SSV @22:40:44.47 CTV.dial :air,avoiding: :intake @22:40:45.73 CSV.dial CTV @22:40:45.76
SS.seek 10ml @22:40:46.76 CSV.dial :air @22:40:47.32 CS.reconfigure :fast @22:40:47.37 CS.seek 0ml @22:40:48.48
SSV.dial :spit,avoiding: :exhaust @22:40:49.78 SS.reconfigure :exhale @22:40:49.82 SS.seek 0ml @22:40:51.99 CS.stop @22:40:55.11
SS.reconfigure :fast @22:40:55.15 SS.seek 5ml @22:40:57.98 SSV.dial CBV,avoiding: :exhaust @22:40:58.01
CBV.dial SSV @22:41:00.30 SSV.dial :spit,avoiding: :exhaust @22:41:01.59 SS.reconfigure :default @22:41:01.63 SS.seek 0ml @22:41:04.94 SS.stop @22:41:04.98 Lysing: Adding 2.0ml AqMeOH @22:41:04.99 CBV.dial between CSV and waste1,avoiding: :DBV @22:41:06.43 CTV.dial CSV @22:41:07.68 CSV.dial CSR @22:41:08.42 Pulling 1ml of CSR.AqMeOH @22:41:08.43 CSR.select :AqMeOH @22:41:08.53 CS.reconfigure :default @22:41:08.57 CS.seek 1ml @22:41:11.96 delay 5 seconds @22:41:16.98 CSR.select :air @22:41:17.13 CS.seek 6ml @22:41:30.52 delay 2 seconds @22:41:32.54 CSV.dial :waste2 @22:41:32.96 CS.reconfigure :fast @22:41:33.01 CS.seek 0ml @22:41:46.74 CSV.dial CSR @22:41:47.34 Pulling 2ml of CSR.AqMeOH @22:41:47.35 CSR.select :AqMeOH @22:41:47.45 CS.reconfigure :default @22:41:47.49 CS.seek 2ml @22:41:53.34 delay 5 seconds @22:41:58.35 CSR.select :air @22:41:58.50 CS.seek 5ml @22:42:06.89 delay 2 seconds @22:42:08.91 CSV.dial CTV @22:42:09.35 CS.seek 1ml @22:42:20.43 Before lysing Collection Puck Heater at 16.9C/38.8psia @22:42:20.45 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @22:42:20.47 CH.seek 70C,8:00 @22:44:06.59 CH.hold 10:00,70C @22:44:06.76 Begun lysing Collection Puck Heater at 68.1C/43.1psia @22:54:06.78 Ended lysing Collection Puck Heater at 70C/43.7psia @22:54:06.79 CS.seek 0ml @22:54:10.13 CTV.dial between spare and air,avoiding: :intake @22:54:11.11 CSV.dial :air @22:54:11.55 CS.seek 5ml @22:54:25.06 CSV.dial CTV @22:54:25.61 delay 2 seconds @22:54:27.63 CSV.dial :air @22:54:28.03 delay 2 seconds @22:54:30.05 CSV.dial CTV @22:54:30.49 delay 2 seconds @22:54:32.50 CSV.dial :air @22:54:32.92 delay 2 seconds @22:54:34.94 CS.seek 0ml @22:54:48.33 Puck @71.2psia after final lysis pressurization @22:54:48.35 Recovering Lysate into Collection syringe CSV.dial CBV @22:54:49.34 CBV.dial CSV @22:54:49.70 CS.seek 5ml @22:55:03.19 delay 5 seconds @22:55:08.20 CTV.dial :air @22:55:08.70 CS.seek 7.001ml @22:55:14.56 delay 5 seconds @22:55:19.58 CSV.dial :air2 @22:55:20.01 CS.seek 10ml @22:55:28.41 CSV.dial between air and CTV,avoiding: CBV @22:55:29.52 Lysing Complete @22:55:29.59 Storing sh1 puck in Collection Clamp to tube 2 @22:55:29.74 SC.reconfigure :tight @22:55:29.78 SC.seek 2 @22:55:33.34 SC.configure :limp @22:55:33.54 Elbow.reconfigure :tight @22:55:33.58 Elbow.seek 2 @22:55:35.27 Forearm.reconfigure :tight @22:55:35.31 Forearm.seek 2 + 700 counts @22:55:36.35 SE.reconfigure :pick @22:55:36.39 SE.seek :beamBreak @22:55:38.65 SE.configure :default @22:55:38.78 Elbow.reconfigure :limp @22:55:38.83 Forearm.reconfigure :loose @22:55:38.87 Forearm.seek 2 @22:55:39.24 Elbow.hold @22:55:39.30 Elbow.reconfigure :tight @22:55:39.39 SE.seek 14.29 Pucks below Top Plate @22:55:39.79 Forearm.force -20 @22:55:39.83 Forearm.seek 2 + 303 counts @22:55:40.63 Forearm.reconfigure :tight @22:55:40.67 Forearm.seek 2 + 700 counts @22:55:40.87 SE.seek 15.29 Pucks below Top Plate @22:55:41.48 Forearm.reconfigure :loose @22:55:41.52 Forearm.seek :retract @22:55:42.43 Elbow.seek CC @22:55:44.12 Elbow.reconfigure :loose @22:55:44.21 Forearm.seek CC @22:55:45.22 Hand.close @22:55:45.95 CC.seek :open @22:55:50.36 Forearm.seek :retract @22:55:51.35 Elbow.reconfigure :tight @22:55:51.39 Elbow.seek 2 @22:55:53.14 Elbow.reconfigure :limp @22:55:53.19 Forearm.seek 2 @22:55:54.30 Elbow.hold @22:55:54.36 Elbow.reconfigure :tight @22:55:54.40 Hand.open @22:55:55.31 Forearm.force -20 @22:55:55.35 Forearm.seek 2 + 320 counts @22:55:55.67 Forearm.reconfigure :tight @22:55:55.71 Forearm.seek 2 + 700 counts @22:55:56.05 SE.seek :down @22:55:57.18
Forearm.reconfigure :loose @22:55:57.22 Forearm.seek :retract @22:55:57.73 SE.stop @22:55:58.10
Tranferring DA @22:55:58.11 Beginning samplePassC @22:55:58.13 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:55:58.30 Elbow.seek FlushPuck::Garage @22:56:00.24 Elbow.reconfigure :loose @22:56:00.33 Forearm.seek :garage @22:56:01.46 Hand.close @22:56:02.18 Forearm.seek :retract @22:56:03.31 Elbow.reconfigure :tight @22:56:03.35 Elbow.seek CC @22:56:06.17 Elbow.reconfigure :loose @22:56:06.26 Forearm.reconfigure :tight @22:56:06.30 Forearm.seek CC @22:56:07.52 CC.seek :closed @22:56:11.73 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.88 @22:56:11.80 Hand.open @22:56:12.63 Forearm.reconfigure :loose @22:56:12.67 Forearm.seek :retract @22:56:13.66 PSV.dial PSR @22:56:14.98 Pulling 1.5ml of PSR.flush @22:56:14.99 PSR.select :flush @22:56:15.10 PS.seek 1.5ml @22:56:31.15 delay 5 seconds @22:56:36.16 PSV.dial :waste1 @22:56:36.60 PS.seek 0ml @22:56:52.61 PSV.dial PSR @22:56:53.05 PSR.select :air @22:56:53.16 PS.seek 2.5ml @22:57:19.40 PRV.dial CBV,avoiding: :ab2 @22:57:19.42
CBV.dial PRV @22:57:21.05 PSV.dial :mixloop @22:57:22.02 CTV.dial :air,avoiding: :intake @22:57:22.03 PTV.dial :PRVmixing @22:57:23.26 PS.seek 0ml @22:57:49.39 PSV.dial :air @22:57:49.82 PS.seek 2.5ml @22:58:16.01 PSV.dial :mixloop @22:58:16.46 PS.seek 0ml @22:58:43.11 PSV.dial :air @22:58:43.55 PS.seek 2.5ml @22:59:09.76 PSV.dial :mixloop @22:59:10.21 PS.seek 0ml @22:59:36.51 Garaging FlushPuck in Collection Clamp @22:59:36.58 Elbow.reconfigure :tight @22:59:36.62 Elbow.seek CC @22:59:36.74 Elbow.reconfigure :loose @22:59:36.83 Forearm.seek CC @22:59:37.89 Hand.close @22:59:38.44 CC.seek :open @22:59:42.76 Forearm.seek :retract @22:59:43.86 Elbow.reconfigure :tight @22:59:43.90 Elbow.seek :garage @22:59:46.88 Elbow.reconfigure :loose @22:59:46.97 Forearm.reconfigure :tight @22:59:47.01 Forearm.seek :garage @22:59:48.12 Hand.open @22:59:48.87 Forearm.reconfigure :loose @22:59:48.91 Forearm.seek :retract @22:59:49.97 PSV.dial :mixloop @22:59:49.98 PTV.dial :PRVmixing @22:59:49.99 PRV.dial CBV @22:59:50.02
CBV.dial PRV @22:59:50.03 CTV.dial :air,avoiding: :intake @22:59:50.05 Loading lyfil puck from tube 3 into Collection Clamp @22:59:50.26 SC.reconfigure :tight @22:59:50.30 SC.seek 3 @22:59:53.87 SC.configure :limp @22:59:54.06 Elbow.reconfigure :tight @22:59:54.10 Elbow.seek 3 @22:59:56.10 Forearm.reconfigure :tight @22:59:56.14 Forearm.seek 3 + 700 counts @22:59:57.21 SE.reconfigure :pick @22:59:57.26 SE.seek :beamBreak @22:59:59.28 SE.configure :default @23:00:00.25 Elbow.seek 3 @23:00:00.62 Forearm.seek 3 + 300 counts @23:00:00.90 Forearm.reconfigure :loose @23:00:00.94 Hand.close @23:00:01.57 SE.seek :down @23:00:01.76
Forearm.seek :retract @23:00:02.77 Elbow.seek CC @23:00:03.32 SE.stop @23:00:04.42
Elbow.reconfigure :loose @23:00:04.51 Forearm.reconfigure :tight @23:00:04.55 Forearm.seek CC @23:00:05.78 CC.seek :closed @23:00:10.02 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.65 @23:00:10.03 Hand.open @23:00:10.88 Forearm.reconfigure :loose @23:00:10.92 Forearm.seek :retract @23:00:11.94 CTV.dial CSV,avoiding: :intake @23:00:13.18 CSV.dial CTV @23:00:13.48 PS.seek 2.5ml @23:00:39.72 CS.seek 5.5ml @23:00:51.83 delay 8 seconds @23:00:59.85 PSV.dial :air @23:01:00.29 delay 2 seconds @23:01:02.31 PSV.dial :waste2 @23:01:03.02 PS.seek 0ml @23:01:29.13 CBV.dial between PRV and waste1 @23:01:29.46 CSV.dial :air @23:01:29.92 CS.seek 10ml @23:01:42.52 CSV.dial CTV @23:01:43.09 CS.seek 0ml @23:02:09.05 CBV.dial :waste1 @23:02:09.35 delay 5 seconds @23:02:14.37 CTV.dial :air,avoiding: :intake @23:02:15.67 Storing lyfil puck in Collection Clamp to tube 2 @23:02:15.83 SC.reconfigure :tight @23:02:15.87 SC.seek 2 @23:02:19.48 SC.configure :limp @23:02:19.67 Elbow.reconfigure :tight @23:02:19.71 Elbow.seek 2 @23:02:21.47 Forearm.reconfigure :tight @23:02:21.51 Forearm.seek 2 + 700 counts @23:02:22.51 SE.reconfigure :pick @23:02:22.56 SE.seek :beamBreak @23:02:24.62 SE.configure :default @23:02:24.77 Elbow.reconfigure :limp @23:02:24.81 Forearm.reconfigure :loose @23:02:24.85 Forearm.seek 2 @23:02:25.23 Elbow.hold @23:02:25.29 Elbow.reconfigure :tight @23:02:25.38 SE.seek 15.29 Pucks below Top Plate @23:02:25.89 Forearm.force -20 @23:02:25.93 Forearm.seek 2 + 311 counts @23:02:26.28 Forearm.reconfigure :tight @23:02:26.32 Forearm.seek 2 + 700 counts @23:02:26.61 SE.seek 16.29 Pucks below Top Plate @23:02:27.23 Forearm.reconfigure :loose @23:02:27.28 Forearm.seek :retract @23:02:28.18 Elbow.seek CC @23:02:29.83 Elbow.reconfigure :loose @23:02:29.92 Forearm.seek CC @23:02:30.94 Hand.close @23:02:31.56 CC.seek :open @23:02:35.99 Forearm.seek :retract @23:02:36.98 Elbow.reconfigure :tight @23:02:37.02 Elbow.seek 2 @23:02:38.71 Elbow.reconfigure :limp @23:02:38.76 Forearm.seek 2 @23:02:39.84 Elbow.hold @23:02:39.90 Elbow.reconfigure :tight @23:02:39.94 Hand.open @23:02:40.80 Forearm.force -20 @23:02:40.83 Forearm.seek 2 + 320 counts @23:02:41.23 Forearm.reconfigure :tight @23:02:41.28 Forearm.seek 2 + 700 counts @23:02:41.70 SE.seek :down @23:02:42.84
Forearm.reconfigure :loose @23:02:42.88 Forearm.seek :retract @23:02:43.30 SE.stop @23:02:43.77
PSV.dial :air @23:02:44.50 PS.seek 2.5ml @23:03:10.69 delay 1 second @23:03:11.71 PSV.dial :mixloop @23:03:12.13 delay 1 second @23:03:13.15 PSV.dial :air @23:03:13.59 delay 1 second @23:03:14.60 PSV.dial :mixloop @23:03:15.04 delay 1 second @23:03:16.05 PSV.dial :air @23:03:16.49 delay 1 second @23:03:17.51 PS.seek 0ml @23:03:43.73 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @23:03:43.89 Elbow.seek FlushPuck::Garage @23:03:45.67 Elbow.reconfigure :loose @23:03:45.76 Forearm.seek :garage @23:03:46.85 Hand.close @23:03:47.54 Forearm.seek :retract @23:03:49.02 Elbow.reconfigure :tight @23:03:49.07 Elbow.seek PC @23:03:50.28 Elbow.reconfigure :loose @23:03:50.37 Forearm.reconfigure :tight @23:03:50.41 Forearm.seek PC @23:03:51.81 PC.seek :closed @23:03:55.71 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.38 @23:03:55.78 Hand.open @23:03:56.61 Forearm.reconfigure :loose @23:03:56.65 Forearm.seek :retract @23:03:57.68 PRV.dial :air @23:03:58.11 PSV.dial :mixloop @23:03:58.61 PS.seek 0.3ml @23:04:02.47 PSV.dial PSR @23:04:03.50 Pulling 0.4ml of PSR.air PSR.select :air @23:04:03.53 PS.seek 0.7ml @23:04:08.38 delay 3 seconds @23:04:11.39 PSR.select :air @23:04:11.42 PS.seek 2.5ml @23:04:30.44 delay 3 seconds @23:04:33.46 PSV.dial :mixloop @23:04:34.44 PTV.dial :mixing @23:04:35.14 PBV.dial :waste2 @23:04:36.03 PS.seek 0ml @23:05:02.22 Garaging FlushPuck in Processing Clamp @23:05:02.29 Elbow.reconfigure :tight @23:05:02.33 Elbow.seek PC @23:05:02.46 Elbow.reconfigure :loose @23:05:02.55 Forearm.seek PC @23:05:03.63 Hand.close @23:05:04.18 PC.seek :open @23:05:08.14 Forearm.seek :retract @23:05:09.18 Elbow.reconfigure :tight @23:05:09.22 Elbow.seek :garage @23:05:10.56 Elbow.reconfigure :loose @23:05:10.64 Forearm.reconfigure :tight @23:05:10.69 Forearm.seek :garage @23:05:11.78 Hand.open @23:05:12.61 Forearm.reconfigure :loose @23:05:12.65 Forearm.seek :retract @23:05:13.72 Loading da puck from tube 3 into Processing Clamp @23:05:13.93 SC.reconfigure :tight @23:05:13.97 SC.seek 3 @23:05:17.50 SC.configure :limp @23:05:17.69 Elbow.reconfigure :tight @23:05:17.73 Elbow.seek 3 @23:05:19.64 Forearm.reconfigure :tight @23:05:19.69 Forearm.seek 3 + 700 counts @23:05:20.73 SE.reconfigure :pick @23:05:20.78 SE.seek :beamBreak @23:05:22.77 SE.configure :default @23:05:22.91 Elbow.seek 3 @23:05:23.08 Forearm.seek 3 + 300 counts @23:05:23.41 Forearm.reconfigure :loose @23:05:23.45 Hand.close @23:05:24.06 SE.seek :down @23:05:24.25
Forearm.seek :retract @23:05:25.19 Elbow.seek PC @23:05:25.91 SE.stop @23:05:27.79
Elbow.reconfigure :loose @23:05:27.88 Forearm.reconfigure :tight @23:05:27.92 Forearm.seek PC @23:05:29.26 PC.seek :closed @23:05:33.13 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.38 @23:05:33.14 Hand.open @23:05:33.98 Forearm.reconfigure :loose @23:05:34.02 Forearm.seek :retract @23:05:35.06 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @23:05:35.07 PH.seek 28C,5:00 @23:06:14.91 PH.hold 2:00:00,28C @23:06:14.97 delay 1:30 @23:07:45.00 PSR.select :air @23:07:45.02 PSV.dial PSR @23:07:46.07 Pulling 0.5ml of PSR.wash @23:07:46.08 PSR.select :wash @23:07:46.19 PS.seek 0.5ml @23:07:52.07 delay 5 seconds @23:07:57.08 PSR.select :air @23:07:57.20 PS.seek 2ml @23:08:13.25 PTV.dial :puck @23:08:13.92 PSV.dial PTV @23:08:14.64 PBV.dial :waste1 @23:08:15.92 PS.seek 1.5ml @23:08:21.81 delay 3 seconds @23:08:24.83 PBV.dial between PSV and waste1 @23:08:25.18 PS.seek 0ml @23:08:41.22 delay 5:00 @23:13:41.24 recoverPSRreagent08 @23:13:41.25 PTV.dial :puck @23:13:41.26 PBV.dial :waste1 @23:13:41.62 delay 30 seconds @23:14:11.64 PBV.dial between PSV and waste1 @23:14:11.99 PSR.select :air @23:14:12.01 PSV.dial PSR @23:14:12.85 PS.reconfigure :fast @23:14:12.90 PS.seek 2.5ml @23:14:35.42 PSV.dial PTV @23:14:36.11 delay 5 seconds @23:14:41.13 PSV.dial PSR @23:14:41.85 delay 5 seconds @23:14:46.87 PSV.dial PTV @23:14:47.58 delay 5 seconds @23:14:52.60 PSV.dial :air @23:14:53.32 PS.seek 0ml @23:15:15.64 PTV.dial :PRVmixing,via: PRV @23:15:16.89 PSV.dial :mixloop @23:15:17.33 PRV.dial :ab1 @23:15:18.07 PS.reconfigure :slow @23:15:18.11 PS.seek 0.15ml @23:15:21.64 delay 2 seconds @23:15:23.66 CBV.dial PRV @23:15:23.71
PRV.dial CBV @23:15:24.22 PS.seek 0.34ml @23:15:28.58 delay 2 seconds @23:15:30.60 CBV.dial between PRV and PSV @23:15:30.91 PRV.dial :air @23:15:31.35 PS.seek 0.9ml @23:15:43.17 PRV.dial :waste2 @23:15:43.88 PS.seek 0.5ml @23:15:52.49 addPMLReagent08 @23:15:52.50 PSV.dial :air @23:15:52.94 PS.reconfigure :default @23:15:52.99 PS.seek 2ml @23:16:08.95 PSV.dial :PML @23:16:09.39 PTV.dial :mixing @23:16:10.09 PBV.dial :waste1 @23:16:10.51 PS.seek 1.85ml @23:16:12.92 PBV.dial between PSV and waste1 @23:16:13.31 PS.seek 0ml @23:16:32.92 delay 2 seconds @23:16:34.94 Beginning samplePassC @23:16:34.95 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @23:16:35.12 Elbow.reconfigure :tight @23:16:35.16 Elbow.seek FlushPuck::Garage @23:16:36.71 Elbow.reconfigure :loose @23:16:36.79 Forearm.seek :garage @23:16:37.90 Hand.close @23:16:39.14 Forearm.seek :retract @23:16:40.20 Elbow.reconfigure :tight @23:16:40.24 Elbow.seek CC @23:16:43.10 Elbow.reconfigure :loose @23:16:43.19 Forearm.reconfigure :tight @23:16:43.23 Forearm.seek CC @23:16:44.52 CC.seek :closed @23:16:48.77 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.81 @23:16:48.83 Hand.open @23:16:49.47 Forearm.reconfigure :loose @23:16:49.51 Forearm.seek :retract @23:16:50.50 PSV.dial PSR @23:16:51.65 Pulling 1.5ml of PSR.flush @23:16:51.66 PSR.select :flush @23:16:51.77 PS.seek 1.5ml @23:17:07.83 delay 5 seconds @23:17:12.85 PSV.dial :waste1 @23:17:13.30 PS.seek 0ml @23:17:29.45 PSV.dial PSR @23:17:30.00 PSR.select :air @23:17:30.11 PS.seek 2.5ml @23:17:56.30 PRV.dial CBV,avoiding: :ab2 @23:17:56.33
CBV.dial PRV @23:17:57.89 PSV.dial :mixloop @23:17:58.89 CTV.dial :air,avoiding: :intake @23:17:58.90 PTV.dial :PRVmixing @23:17:59.61 PS.seek 0ml @23:18:25.79 PSV.dial :air @23:18:26.22 PS.seek 2.5ml @23:18:52.45 PSV.dial :mixloop @23:18:52.89 PS.seek 0ml @23:19:19.35 PSV.dial :air @23:19:19.80 PS.seek 2.5ml @23:19:45.92 PSV.dial :mixloop @23:19:46.36 PS.seek 0ml @23:20:12.51 Garaging FlushPuck in Collection Clamp @23:20:12.58 Elbow.reconfigure :tight @23:20:12.62 Elbow.seek CC @23:20:12.73 Elbow.reconfigure :loose @23:20:12.82 Forearm.seek CC @23:20:13.77 Hand.close @23:20:14.40 CC.seek :open @23:20:18.72 Forearm.seek :retract @23:20:19.73 Elbow.reconfigure :tight @23:20:19.77 Elbow.seek :garage @23:20:22.65 Elbow.reconfigure :loose @23:20:22.74 Forearm.reconfigure :tight @23:20:22.78 Forearm.seek :garage @23:20:23.91 Hand.open @23:20:24.70 Forearm.reconfigure :loose @23:20:24.74 Forearm.seek :retract @23:20:25.76 delayUntil "23:24:34.93" @23:20:25.78 Whole Cell Archive sampling 1000ml @23:20:25.80 Loading wcr puck from tube 3 into Collection Clamp @23:20:26.23 Elbow.reconfigure :tight @23:20:26.27 Elbow.seek 3 @23:20:28.24 Forearm.reconfigure :tight @23:20:28.29 Forearm.seek 3 + 700 counts @23:20:29.27 SE.reconfigure :pick @23:20:29.33 SE.seek :beamBreak @23:20:31.45 SE.configure :default @23:20:31.60 Elbow.seek 3 @23:20:32.24 Forearm.seek 3 + 300 counts @23:20:32.47 Forearm.reconfigure :loose @23:20:32.52 Hand.close @23:20:33.16 SE.seek :down @23:20:33.36 Forearm.seek :retract @23:20:34.37 Elbow.seek CC @23:20:35.03 SE.stop @23:20:35.97 Elbow.reconfigure :loose @23:20:36.07 Forearm.reconfigure :tight @23:20:36.11 Forearm.seek CC @23:20:37.34 CC.seek :closed @23:20:41.57 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.66 @23:20:41.58 Hand.open @23:20:42.33 Forearm.reconfigure :loose @23:20:42.37 Forearm.seek :retract @23:20:43.41 collectSample 1000ml w/bubble point of 35psi @23:20:43.43 Priming 7ml Intake.select :close @23:20:43.44 Exhaust.select :close @23:20:43.45 SS.hold @23:20:43.64 Calibrating pressure sensors @23:20:43.65 CTV.dial :air @23:20:43.67 SSV.dial :spit,avoiding: :exhaust @23:20:43.68 SS.reconfigure :fast @23:20:43.73 SS.seek 5ml @23:20:46.48 SSV.dial CBV @23:20:46.52 CBV.dial SSV @23:20:47.78 delay 3.8 seconds @23:20:51.74 SSV.dial :spit @23:20:53.03 SS.seek 0ml @23:20:55.88 SSV.dial CBV @23:20:57.15 CTV.dial :intake @23:20:57.87 SSV.dial CBV @23:20:57.89 CBV.dial SSV @23:20:57.91 Allowing 40s for 7.0ml of remaining 7.0ml @23:20:57.92 Intake.select :open @23:21:00.07 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:21:00.09 SS.reconfigure :inhaling 2.14ml/s @23:21:00.13 SS.seek 7ml @23:21:06.91 Stroke completed -- waiting for pressure to equalize ... @23:21:06.92 SS.reconfigure :exhale @23:21:06.97 SS.hold @23:21:27.40 Intake.select :close @23:21:27.54 SSV.dial :exhaust @23:21:28.28 Exhaust.select :open @23:21:28.39 SS.seek 0ml @23:21:32.77 Exhaust.select :close @23:21:32.88 Sampling 1000.0ml @23:21:32.89 Intake.select :close @23:21:32.90 Exhaust.select :close @23:21:32.91 SS.reconfigure :default @23:21:32.95 SS.hold @23:21:33.14 CTV.dial :intake @23:21:33.15 SSV.dial CBV @23:21:33.18 CBV.dial SSV @23:21:33.92 Allowing 130s for 25.0ml of remaining 1000.0ml @23:21:33.93 Intake.select :open @23:21:36.56 Intake @15.9psia, vacuum from 10.0 to 13.0psi @23:21:36.58 SS.reconfigure :inhaling 2.14ml/s @23:21:36.62 SS.seek 25ml @23:21:40.27 Vacuum too high -- waiting up to 40 seconds @23:21:40.28 SS.reconfigure :exhale @23:21:40.32 SS.hold @23:21:43.06 Intake @15.1psia, vacuum from 9.6 to 12.6psi @23:21:43.08 SS.reconfigure :inhaling 1.93ml/s @23:21:43.13 SS.seek 25ml @23:21:45.55 Vacuum too high -- waiting up to 40 seconds @23:21:45.56 SS.reconfigure :exhale @23:21:45.61 SS.hold @23:21:48.84 Intake @17.2psia, vacuum from 10.0 to 13.0psi @23:21:48.85 SS.reconfigure :inhaling 1.74ml/s @23:21:48.90 SS.seek 25ml @23:21:50.89 Vacuum too high -- waiting up to 40 seconds @23:21:50.90 SS.reconfigure :exhale @23:21:50.94 SS.hold @23:21:51.66 Intake @15.1psia, vacuum from 9.6 to 12.6psi @23:21:51.68 SS.reconfigure :inhaling 1.57ml/s @23:21:51.73 SS.seek 25ml @23:21:53.89 Vacuum too high -- waiting up to 40 seconds @23:21:53.90 SS.reconfigure :exhale @23:21:53.95 SS.hold @23:21:56.59 Intake @17.3psia, vacuum from 10.0 to 13.0psi @23:21:56.61 SS.reconfigure :inhaling 1.41ml/s @23:21:56.65 SS.seek 25ml @23:21:58.14 Vacuum too high -- waiting up to 40 seconds @23:21:58.15 SS.reconfigure :exhale @23:21:58.19 SS.hold @23:21:59.54 Intake @16.6psia, vacuum from 10.0 to 13.0psi @23:21:59.56 SS.reconfigure :inhaling 1.27ml/s @23:21:59.60 SS.seek 25ml @23:22:01.36 Vacuum too high -- waiting up to 40 seconds @23:22:01.38 SS.reconfigure :exhale @23:22:01.42 SS.hold @23:22:03.09 Intake @16.3psia, vacuum from 10.0 to 13.0psi @23:22:03.11 SS.reconfigure :inhaling 1.14ml/s @23:22:03.15 SS.seek 25ml @23:22:05.20 Vacuum too high -- waiting up to 40 seconds @23:22:05.21 SS.reconfigure :exhale @23:22:05.26 SS.hold @23:22:06.93 Intake @16.0psia, vacuum from 10.0 to 13.0psi @23:22:06.95 SS.reconfigure :inhaling 1.03ml/s @23:22:06.99 SS.seek 25ml @23:22:09.48 Vacuum too high -- waiting up to 40 seconds @23:22:09.50 SS.reconfigure :exhale @23:22:09.54 SS.hold @23:22:11.18 Intake @16.0psia, vacuum from 10.0 to 13.0psi @23:22:11.20 SS.reconfigure :inhaling 0.93ml/s @23:22:11.24 SS.seek 25ml @23:22:14.45 Vacuum too high -- waiting up to 40 seconds @23:22:14.46 SS.reconfigure :exhale @23:22:14.50 SS.hold @23:22:16.14 Intake @16.2psia, vacuum from 10.0 to 13.0psi @23:22:16.16 SS.reconfigure :inhaling 0.84ml/s @23:22:16.20 SS.seek 25ml @23:22:19.33 Vacuum too high -- waiting up to 40 seconds @23:22:19.82 SS.reconfigure :exhale @23:22:19.87 SS.hold @23:22:21.03 Intake @16.3psia, vacuum from 10.0 to 13.0psi @23:22:21.04 SS.reconfigure :inhaling 0.76ml/s @23:22:21.09 SS.seek 25ml @23:22:23.95 Vacuum too high -- waiting up to 40 seconds @23:22:23.96 SS.reconfigure :exhale @23:22:24.01 SS.hold @23:22:24.97 Intake @15.9psia, vacuum from 10.0 to 13.0psi @23:22:24.98 SS.reconfigure :inhaling 0.69ml/s @23:22:25.03 SS.seek 25ml @23:22:28.94 Stroke completed -- waiting for pressure to equalize ... @23:22:28.95 SS.reconfigure :exhale @23:22:28.99 SS.hold @23:22:32.53 Intake.select :close @23:22:32.68 SSV.dial :exhaust @23:22:33.41 Exhaust.select :open @23:22:33.52 SS.seek 0ml @23:22:42.80 Exhaust.select :close @23:22:42.91 SSV.dial CBV @23:22:42.93 CBV.dial SSV @23:22:43.68 Allowing 130s for 25.0ml of remaining 975.0ml @23:22:43.69 Intake.select :open @23:22:45.84 Intake @15.7psia, vacuum from 10.0 to 13.0psi @23:22:45.86 SS.reconfigure :inhaling 0.69ml/s @23:22:45.91 SS.seek 25ml @23:22:53.39 Vacuum too high -- waiting up to 40 seconds @23:22:53.40 SS.reconfigure :exhale @23:22:53.44 SS.hold @23:22:55.08 Intake @16.4psia, vacuum from 10.0 to 13.0psi @23:22:55.10 SS.reconfigure :inhaling 0.61ml/s @23:22:55.14 SS.seek 25ml @23:23:00.07 Vacuum too high -- waiting up to 40 seconds @23:23:00.09 SS.reconfigure :exhale @23:23:00.13 SS.hold @23:23:01.08 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:23:01.10 SS.reconfigure :inhaling 0.56ml/s @23:23:01.14 SS.seek 25ml @23:23:07.79 Vacuum too high -- waiting up to 40 seconds @23:23:07.81 SS.reconfigure :exhale @23:23:07.85 SS.hold @23:23:09.12 Intake @16.6psia, vacuum from 10.0 to 13.0psi @23:23:09.14 SS.reconfigure :inhaling 0.50ml/s @23:23:09.18 SS.seek 25ml @23:23:21.14 Vacuum too high -- waiting up to 40 seconds @23:23:21.15 SS.reconfigure :exhale @23:23:21.19 SS.hold @23:23:22.36 Intake @16.5psia, vacuum from 10.0 to 13.0psi @23:23:22.38 SS.reconfigure :inhaling 0.46ml/s @23:23:22.42 SS.seek 25ml @23:23:43.95 Stroke completed -- waiting for pressure to equalize ... @23:23:43.97 SS.reconfigure :exhale @23:23:44.01 SS.hold @23:23:47.55 Intake.select :close @23:23:47.69 SSV.dial :exhaust @23:23:48.43 Exhaust.select :open @23:23:48.54 SS.seek 0ml @23:23:57.56 Exhaust.select :close @23:23:57.67 SSV.dial CBV @23:23:57.72 CBV.dial SSV @23:23:58.41 Allowing 130s for 25.0ml of remaining 950.0ml @23:23:58.42 Intake.select :open @23:24:01.06 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:24:01.07 SS.reconfigure :inhaling 0.46ml/s @23:24:01.12 SS.seek 25ml @23:24:34.94 recoverPRVreagent08 @23:24:34.95 PTV.dial :mixing @23:24:35.66 PBV.dial :waste1 @23:24:36.02 delay 30 seconds @23:25:02.39 Stroke completed -- waiting for pressure to equalize ... @23:25:02.40 SS.reconfigure :exhale @23:25:02.44 SS.hold @23:25:06.03 PBV.dial between PSV and waste1 @23:25:06.39 PSV.dial :air @23:25:06.85 PS.reconfigure :fast @23:25:06.89 PS.seek 2.5ml @23:25:07.30 Intake.select :close @23:25:07.45 SSV.dial :exhaust @23:25:08.17 Exhaust.select :open @23:25:08.28 SS.seek 0ml @23:25:17.69 Exhaust.select :close @23:25:17.79 SSV.dial CBV @23:25:17.82 CBV.dial SSV @23:25:18.58 Allowing 130s for 25.0ml of remaining 925.0ml @23:25:18.59 Intake.select :open @23:25:20.75 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:25:20.76 SS.reconfigure :inhaling 0.46ml/s @23:25:20.81 SS.seek 25ml @23:25:29.72 PSV.dial :mixloop @23:25:30.17 delay 2 seconds @23:25:32.19 PSV.dial :air @23:25:32.64 delay 2 seconds @23:25:34.66 PSV.dial :mixloop @23:25:35.11 delay 2 seconds @23:25:37.13 PSV.dial :air @23:25:37.57 PS.seek 0ml @23:26:00.06 washCycleDA #1 of 2 @23:26:00.07 PSR.select :air @23:26:00.09 PSV.dial PSR @23:26:01.52 Pulling 0.5ml of PSR.wash @23:26:01.53 PSR.select :wash @23:26:01.64 PS.reconfigure :default @23:26:01.68 PS.seek 0.5ml @23:26:07.63 delay 5 seconds @23:26:07.79 Vacuum too high -- waiting up to 40 seconds @23:26:07.80 SS.reconfigure :exhale @23:26:07.84 SS.hold @23:26:11.27 Intake @15.9psia, vacuum from 10.0 to 13.0psi @23:26:11.29 SS.reconfigure :inhaling 0.41ml/s @23:26:11.33 SS.seek 25ml @23:26:12.64 PSR.select :air @23:26:12.76 PS.seek 2ml @23:26:25.65 Stroke completed -- waiting for pressure to equalize ... @23:26:25.66 SS.reconfigure :exhale @23:26:25.71 SS.hold @23:26:28.81 PTV.dial :puck @23:26:29.50 PSV.dial PTV @23:26:30.20 PBV.dial :waste1 @23:26:30.60 PS.seek 1.5ml @23:26:31.38 Intake.select :close @23:26:31.58 SSV.dial :exhaust @23:26:32.31 Exhaust.select :open @23:26:32.42 SS.seek 0ml @23:26:36.54 delay 3 seconds @23:26:39.55 PBV.dial between PSV and waste1 @23:26:39.91 PS.seek 0ml @23:26:41.82 Exhaust.select :close @23:26:41.93 SSV.dial CBV @23:26:41.96 CBV.dial SSV @23:26:42.69 Allowing 130s for 25.0ml of remaining 900.0ml @23:26:42.70 Intake.select :open @23:26:44.85 Intake @15.5psia, vacuum from 10.0 to 13.0psi @23:26:44.87 SS.reconfigure :inhaling 0.41ml/s @23:26:44.91 SS.seek 25ml @23:26:55.91 delay 2:00 @23:27:52.37 Stroke completed -- waiting for pressure to equalize ... @23:27:52.38 SS.reconfigure :exhale @23:27:52.42 SS.hold @23:28:03.46 Intake.select :close @23:28:03.60 SSV.dial :exhaust @23:28:04.32 Exhaust.select :open @23:28:04.43 SS.seek 0ml @23:28:13.76 Exhaust.select :close @23:28:13.87 SSV.dial CBV @23:28:13.89 CBV.dial SSV @23:28:14.64 Allowing 130s for 25.0ml of remaining 875.0ml @23:28:14.65 Intake.select :open @23:28:16.80 Intake @15.6psia, vacuum from 10.0 to 13.0psi @23:28:16.82 SS.reconfigure :inhaling 0.41ml/s @23:28:16.86 SS.seek 25ml @23:28:37.78 Vacuum too high -- waiting up to 40 seconds @23:28:37.79 SS.reconfigure :exhale @23:28:37.84 SS.hold @23:28:41.38 Intake @15.9psia, vacuum from 10.0 to 13.0psi @23:28:41.40 SS.reconfigure :inhaling 0.37ml/s @23:28:41.44 SS.seek 25ml @23:28:56.39 recoverPSRreagent08 @23:28:56.40 PTV.dial :puck @23:28:56.41 PBV.dial :waste1 @23:28:56.76 delay 30 seconds @23:29:00.31 Vacuum too high -- waiting up to 40 seconds @23:29:00.32 SS.reconfigure :exhale @23:29:00.37 SS.hold @23:29:04.41 Intake @16.0psia, vacuum from 10.0 to 13.0psi @23:29:04.43 SS.reconfigure :inhaling 0.33ml/s @23:29:04.47 SS.seek 25ml @23:29:26.79 PBV.dial between PSV and waste1 @23:29:27.14 PSR.select :air @23:29:27.15 PSV.dial PSR @23:29:27.89 PS.reconfigure :fast @23:29:27.93 PS.seek 2.5ml @23:29:33.47 Vacuum too high -- waiting up to 40 seconds @23:29:33.48 SS.reconfigure :exhale @23:29:33.52 SS.hold @23:29:38.38 Intake @16.1psia, vacuum from 10.0 to 13.0psi @23:29:38.39 SS.reconfigure :inhaling 0.30ml/s @23:29:38.44 SS.seek 25ml @23:29:50.18 Stroke completed -- waiting for pressure to equalize ... @23:29:50.19 SS.reconfigure :exhale @23:29:50.23 SS.hold @23:29:50.52 PSV.dial PTV @23:29:51.24 delay 5 seconds @23:29:56.26 PSV.dial PSR @23:29:56.98 delay 5 seconds @23:29:59.01 Intake.select :close @23:29:59.15 SSV.dial :exhaust @23:29:59.90 Exhaust.select :open @23:30:00.01 SS.seek 0ml @23:30:01.99 PSV.dial PTV @23:30:02.71 delay 5 seconds @23:30:07.73 PSV.dial :air @23:30:08.45 PS.seek 0ml @23:30:09.55 Exhaust.select :close @23:30:09.66 SSV.dial CBV @23:30:09.70 CBV.dial SSV @23:30:10.43 Allowing 130s for 25.0ml of remaining 850.0ml @23:30:10.44 Intake.select :open @23:30:12.59 Intake @15.7psia, vacuum from 10.0 to 13.0psi @23:30:12.61 SS.reconfigure :inhaling 0.30ml/s @23:30:12.66 SS.seek 25ml @23:30:30.87 washCycleDA #2 of 2 @23:30:30.88 PSR.select :air @23:30:30.89 PSV.dial PSR @23:30:32.20 Pulling 0.5ml of PSR.wash @23:30:32.21 PSR.select :wash @23:30:32.32 PS.reconfigure :default @23:30:32.36 PS.seek 0.5ml @23:30:38.25 delay 5 seconds @23:30:43.26 PSR.select :air @23:30:43.38 PS.seek 2ml @23:30:59.46 PTV.dial :puck @23:30:59.47 PSV.dial PTV @23:31:00.18 PBV.dial :waste1 @23:31:00.60 PS.seek 1.5ml @23:31:06.55 delay 3 seconds @23:31:09.57 PBV.dial between PSV and waste1 @23:31:09.94 PS.seek 0ml @23:31:13.21 Vacuum too high -- waiting up to 40 seconds @23:31:13.23 SS.reconfigure :exhale @23:31:13.27 SS.hold @23:31:20.72 Intake @17.1psia, vacuum from 10.0 to 13.0psi @23:31:20.74 SS.reconfigure :inhaling 0.27ml/s @23:31:20.78 SS.seek 25ml @23:31:25.96 delay 2:00 @23:31:30.68 Vacuum too high -- waiting up to 40 seconds @23:31:30.69 SS.reconfigure :exhale @23:31:30.74 SS.hold @23:31:32.67 Intake @16.7psia, vacuum from 10.0 to 13.0psi @23:31:32.69 SS.reconfigure :inhaling 0.24ml/s @23:31:32.73 SS.seek 25ml @23:32:04.21 Vacuum too high -- waiting up to 40 seconds @23:32:04.23 SS.reconfigure :exhale @23:32:04.27 SS.hold @23:32:09.79 Intake @16.8psia, vacuum from 10.0 to 13.0psi @23:32:09.81 SS.reconfigure :inhaling 0.21ml/s @23:32:09.85 SS.seek 25ml @23:32:15.11 Stroke completed -- waiting for pressure to equalize ... @23:32:15.12 SS.reconfigure :exhale @23:32:15.16 SS.hold @23:32:20.45 SS.reconfigure :default @23:32:20.50 SS.hold @23:32:20.61 Intake.select :close @23:32:20.76 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 175.0ml @23:32:20.77 SSV.dial :exhaust @23:32:22.00 Exhaust.select :open @23:32:22.11 SS.reconfigure :exhale @23:32:22.16 SS.seek 0ml @23:32:31.69 Exhaust.select :close @23:32:31.79 Sampled 175.0ml @23:32:31.81 Drying puck before fixing for 20:00 @23:32:31.82 CBV.dial :waste2b @23:32:32.54 delay 1:00 @23:33:25.98 recoverPSRreagent08 @23:33:25.99 PTV.dial :puck @23:33:26.00 PBV.dial :waste1 @23:33:26.36 delay 30 seconds @23:33:32.56 CSV.dial :air @23:33:32.98 CS.reconfigure :fast @23:33:33.02 CS.seek 10ml @23:33:55.50 Pumping wcr puck from tube 3 up to 35psi... @23:33:55.52 CBV.dial SSV @23:33:56.38 PBV.dial between PSV and waste1 @23:33:56.74 PSR.select :air @23:33:56.76 PSV.dial PSR @23:33:56.85 CBV.dial between CSV and waste2b @23:33:57.60 PS.reconfigure :fast @23:33:57.65 PS.seek 2.5ml @23:33:57.76 CSV.dial between air and CTV @23:33:58.20 CS.seek 7.286ml @23:34:04.91 CTV.dial CSV,avoiding: :air @23:34:04.94 CSV.dial CTV @23:34:05.68 CS.reconfigure :pressurize @23:34:05.72 CS.seek 0ml @23:34:19.18 CBV.dial :waste2b @23:34:19.50 CS.reconfigure :default @23:34:19.55 CS.seek 0ml @23:34:20.24 PSV.dial PTV @23:34:20.94 delay 5 seconds @23:34:25.81 delay 1:00 @23:34:25.95 PSV.dial PSR @23:34:26.71 delay 5 seconds @23:34:31.73 PSV.dial PTV @23:34:32.44 delay 5 seconds @23:34:37.46 PSV.dial :air @23:34:38.18 PS.seek 0ml @23:35:00.58 getPMLReagent08 @23:35:00.59 PRV.dial :air @23:35:01.02 delay 5 seconds @23:35:06.04 PTV.dial :PRVmixing @23:35:07.27 delay 5 seconds @23:35:12.29 PRV.dial :ab2 @23:35:12.73 PTV.dial :PRVmixing @23:35:12.75 PSV.dial :mixloop @23:35:13.19 PS.reconfigure :default @23:35:13.23 PS.seek 0.3ml @23:35:17.14 delay 2 seconds @23:35:19.16 PRV.dial :air @23:35:19.65 PS.seek 0.55ml @23:35:22.99 addPMLReagent08 @23:35:23.00 PSV.dial :air @23:35:23.44 PS.seek 2ml @23:35:25.83 CTV.dial :air,avoiding: :intake @23:35:27.10 Fix #1 of 2 (with RNAlater) for 10:00 @23:35:27.11 CSV.dial CSR @23:35:27.58 Pulling 2ml of CSR.RNAlater @23:35:27.59 CSR.select :RNAlater @23:35:27.70 CS.seek 2ml @23:35:33.56 delay 5 seconds @23:35:38.58 CSR.select :air @23:35:38.69 CS.reconfigure :fast @23:35:38.74 CS.seek 10ml @23:35:38.92 PSV.dial :PML @23:35:39.37 PTV.dial :mixing @23:35:40.09 PBV.dial :waste1 @23:35:40.50 PS.seek 1.85ml @23:35:42.92 PBV.dial between PSV and waste1 @23:35:43.29 PS.seek 0ml @23:35:56.88 Pumping wcr puck from tube 3 up to 35psi... @23:35:56.89 CBV.dial SSV @23:35:58.15 CBV.dial between CSV and waste2b @23:35:59.07 CSV.dial between air and CTV @23:35:59.66 CS.seek 7.286ml @23:36:02.86 delay 2 seconds @23:36:04.88 delay 8:00 @23:36:06.34 CTV.dial CSV,avoiding: :air @23:36:06.37 CSV.dial CTV @23:36:08.15 CS.reconfigure :pressurize @23:36:08.20 CS.seek 0ml @23:36:25.17 delay 10:00 @23:44:04.90 recoverPRVreagent08 @23:44:04.91 PTV.dial :mixing @23:44:04.92 PBV.dial :waste1 @23:44:05.27 delay 30 seconds @23:44:35.29 PBV.dial between PSV and waste1 @23:44:35.64 PSV.dial :air @23:44:36.10 PS.reconfigure :fast @23:44:36.14 PS.seek 2.5ml @23:44:58.72 PSV.dial :mixloop @23:44:59.16 delay 2 seconds @23:45:01.17 PSV.dial :air @23:45:01.63 delay 2 seconds @23:45:03.64 PSV.dial :mixloop @23:45:04.08 delay 2 seconds @23:45:06.09 PSV.dial :air @23:45:06.55 PS.seek 0ml @23:45:29.03 washCycleDA #1 of 3 @23:45:29.04 PSR.select :air @23:45:29.06 PSV.dial PSR @23:45:30.36 Pulling 0.5ml of PSR.wash @23:45:30.37 PSR.select :wash @23:45:30.49 PS.reconfigure :default @23:45:30.53 PS.seek 0.5ml @23:45:36.41 delay 5 seconds @23:45:41.43 PSR.select :air @23:45:41.54 PS.seek 2ml @23:45:57.59 PTV.dial :puck @23:45:58.28 PSV.dial PTV @23:45:59.11 PBV.dial :waste1 @23:45:59.51 PS.seek 1.5ml @23:46:05.40 delay 3 seconds @23:46:08.42 PBV.dial between PSV and waste1 @23:46:08.79 PS.seek 0ml @23:46:24.90 delay 2:00 @23:46:25.19 CBV.dial :waste2b @23:46:25.52 CS.reconfigure :default @23:46:25.56 CS.seek 0ml @23:46:28.14 delay 1:00 @23:47:28.16 CTV.dial :air,avoiding: :intake @23:47:29.42 Fix #2 of 2 (with RNAlater) for 10:00 @23:47:29.43 CSV.dial CSR @23:47:29.91 Pulling 2ml of CSR.RNAlater @23:47:29.92 CSR.select :RNAlater @23:47:30.03 CS.seek 2ml @23:47:35.95 delay 5 seconds @23:47:40.97 CSR.select :air @23:47:41.08 CS.reconfigure :fast @23:47:41.12 CS.seek 10ml @23:47:59.17 Pumping wcr puck from tube 3 up to 35psi... @23:47:59.18 CBV.dial SSV @23:48:00.45 CBV.dial between CSV and waste2b @23:48:01.36 CSV.dial between air and CTV @23:48:01.96 CS.seek 7.185ml @23:48:08.89 CTV.dial CSV,avoiding: :air @23:48:08.92 CSV.dial CTV @23:48:10.20 CS.reconfigure :pressurize @23:48:10.25 CS.seek 0ml @23:48:24.92 recoverPSRreagent08 @23:48:24.93 PTV.dial :puck @23:48:24.95 PBV.dial :waste1 @23:48:25.31 delay 30 seconds @23:48:27.09 delay 10:00 @23:48:55.33 PBV.dial between PSV and waste1 @23:48:55.70 PSR.select :air @23:48:55.71 PSV.dial PSR @23:48:56.42 PS.reconfigure :fast @23:48:56.46 PS.seek 2.5ml @23:49:19.08 PSV.dial PTV @23:49:19.81 delay 5 seconds @23:49:24.82 PSV.dial PSR @23:49:25.54 delay 5 seconds @23:49:30.56 PSV.dial PTV @23:49:31.28 delay 5 seconds @23:49:36.29 PSV.dial :air @23:49:37.01 PS.seek 0ml @23:49:59.34 washCycleDA #2 of 3 @23:49:59.35 PSR.select :air @23:49:59.36 PSV.dial PSR @23:50:00.78 Pulling 0.5ml of PSR.wash @23:50:00.79 PSR.select :wash @23:50:00.90 PS.reconfigure :default @23:50:00.94 PS.seek 0.5ml @23:50:06.84 delay 5 seconds @23:50:11.86 PSR.select :air @23:50:11.97 PS.seek 2ml @23:50:27.93 PTV.dial :puck @23:50:27.94 PSV.dial PTV @23:50:28.66 PBV.dial :waste1 @23:50:29.08 PS.seek 1.5ml @23:50:35.02 delay 3 seconds @23:50:38.04 PBV.dial between PSV and waste1 @23:50:38.42 PS.seek 0ml @23:50:54.87 delay 2:00 @23:52:54.89 recoverPSRreagent08 @23:52:54.90 PTV.dial :puck @23:52:54.91 PBV.dial :waste1 @23:52:55.27 delay 30 seconds @23:53:25.29 PBV.dial between PSV and waste1 @23:53:25.64 PSR.select :air @23:53:25.66 PSV.dial PSR @23:53:26.38 PS.reconfigure :fast @23:53:26.43 PS.seek 2.5ml @23:53:49.05 PSV.dial PTV @23:53:49.77 delay 5 seconds @23:53:54.79 PSV.dial PSR @23:53:55.50 delay 5 seconds @23:54:00.52 PSV.dial PTV @23:54:01.24 delay 5 seconds @23:54:06.25 PSV.dial :air @23:54:06.97 PS.seek 0ml @23:54:29.30 washCycleDA #3 of 3 @23:54:29.31 PSR.select :air @23:54:29.32 PSV.dial PSR @23:54:30.62 Pulling 0.5ml of PSR.wash @23:54:30.63 PSR.select :wash @23:54:30.74 PS.reconfigure :default @23:54:30.78 PS.seek 0.5ml @23:54:36.70 delay 5 seconds @23:54:41.72 PSR.select :air @23:54:41.83 PS.seek 2ml @23:54:57.92 PTV.dial :puck @23:54:57.93 PSV.dial PTV @23:54:58.64 PBV.dial :waste1 @23:54:59.06 PS.seek 1.5ml @23:55:04.95 delay 3 seconds @23:55:07.97 PBV.dial between PSV and waste1 @23:55:08.34 PS.seek 0ml @23:55:24.36 delay 2:00 @23:57:24.38 recoverPSRreagent08 @23:57:24.39 PTV.dial :puck @23:57:24.40 PBV.dial :waste1 @23:57:24.76 delay 30 seconds @23:57:54.78 PBV.dial between PSV and waste1 @23:57:55.14 PSR.select :air @23:57:55.15 PSV.dial PSR @23:57:55.87 PS.reconfigure :fast @23:57:55.92 PS.seek 2.5ml @23:58:18.44 PSV.dial PTV @23:58:19.17 delay 5 seconds @23:58:24.19 PSV.dial PSR @23:58:24.90 delay 5 seconds @23:58:27.11 CBV.dial :waste2b @23:58:27.43 CS.reconfigure :default @23:58:27.48 CS.seek 0ml @23:58:29.92 PSV.dial PTV @23:58:29.97 delay 1:00 @23:58:30.67 delay 5 seconds @23:58:35.69 PSV.dial :air @23:58:36.40 PS.seek 0ml @23:58:58.76 Gate.power :camera,:ON @23:58:58.77 Mixing DA Substrates @23:58:58.78 PSR.select :air @23:58:58.79 PSV.dial PSR @23:59:00.36 Pulling 0.25ml of PSR.sub1 @23:59:00.41 PSR.select :sub1 @23:59:00.70 PS.reconfigure :default @23:59:00.92 PS.seek 0.25ml @23:59:04.39 delay 5 seconds @23:59:09.46 Pulling 0.25ml of PSR.sub2 @23:59:09.47 PSR.select :sub2 @23:59:09.59 PS.seek 0.5ml @23:59:13.01 delay 5 seconds @23:59:18.03 PSR.select :air @23:59:18.13 PS.seek 2ml @23:59:30.01 CTV.dial :air,avoiding: :intake @23:59:34.13 PTV.dial :puck @23:59:34.14 PSV.dial PTV @23:59:34.85 PBV.dial :waste1 @23:59:35.27 PS.seek 1.5ml @23:59:41.13 delay 3 seconds @23:59:44.15 PBV.dial between PSV and waste1 @23:59:44.51 PS.seek 0ml @00:00:00.51UTC08-Mar-23 delay 10 seconds @00:00:10.53 recoverPSRreagent08 @00:00:10.54 PTV.dial :puck @00:00:10.55 PBV.dial :waste1 @00:00:10.90 delay 30 seconds @00:00:40.92 PBV.dial between PSV and waste1 @00:00:41.27 PSR.select :air @00:00:41.29 PSV.dial PSR @00:00:42.03 PS.reconfigure :fast @00:00:42.07 PS.seek 2.5ml @00:01:04.60 PSV.dial PTV @00:01:05.43 delay 5 seconds @00:01:10.45 PSV.dial PSR @00:01:11.15 delay 5 seconds @00:01:16.16 PSV.dial PTV @00:01:16.88 delay 5 seconds @00:01:21.90 PSV.dial :air @00:01:22.62 PS.seek 0ml @00:01:45.00 Additional air clear @00:01:45.01 PSR.select :air @00:01:45.03 PSV.dial PSR @00:01:46.34 Pulling 2ml of PSR.air @00:01:46.35 PSR.select :air @00:01:46.37 PS.reconfigure :default @00:01:46.41 PS.seek 2ml @00:02:07.47 delay 5 seconds @00:02:12.49 PTV.dial :puck @00:02:12.50 PSV.dial PTV @00:02:13.22 PBV.dial :waste1 @00:02:13.64 PS.seek 1.5ml @00:02:19.53 delay 3 seconds @00:02:22.55 PBV.dial between PSV and waste1 @00:02:22.91 delay 1 second @00:02:24.41 PSV.dial :waste1 @00:02:25.43 PS.seek 0ml @00:02:41.46 Processing Puck Heater at 28C before imaging @00:02:41.47 PH.coast @00:02:41.59 Imaging da puck in Processing Clamp while storing it to tube 2 @00:02:41.75 SC.reconfigure :tight @00:02:41.79 SC.seek 2 @00:02:45.36 SC.configure :limp @00:02:45.55 Elbow.reconfigure :tight @00:02:45.59 Elbow.seek 2 @00:02:47.36 Forearm.reconfigure :tight @00:02:47.40 Forearm.seek 2 + 700 counts @00:02:48.40 SE.reconfigure :pick @00:02:48.45 SE.seek :beamBreak @00:02:50.51 SE.configure :default @00:02:50.65 Elbow.reconfigure :limp @00:02:50.69 Forearm.reconfigure :loose @00:02:50.73 Forearm.seek 2 @00:02:51.15 Elbow.hold @00:02:51.21 Elbow.reconfigure :tight @00:02:51.30 SE.seek 16.3 Pucks below Top Plate @00:02:51.76 Forearm.force -20 @00:02:51.80 Forearm.seek 2 + 306 counts @00:02:52.16 Forearm.reconfigure :tight @00:02:52.20 Forearm.seek 2 + 700 counts @00:02:52.53 SE.seek 17.3 Pucks below Top Plate @00:02:53.15 Forearm.reconfigure :loose @00:02:53.19 Forearm.seek :retract @00:02:54.13 Elbow.seek PC @00:02:56.66 Elbow.reconfigure :loose @00:02:56.75 Forearm.seek PC @00:02:57.84 Hand.close @00:02:58.44 PC.seek :open @00:03:02.45 Forearm.seek :retract @00:03:03.47 Elbow.reconfigure :tight @00:03:03.51 Elbow.seek 2 @00:03:06.30 Forearm.reconfigure :tight @00:03:06.34 Forearm.seek 2 + 700 counts @00:03:07.31 Hand.open @00:03:08.12 SE.seek 16.95 Pucks below Top Plate @00:03:08.39 SE.reconfigure :seal @00:03:08.44 SE.seek 15.84 Pucks below Top Plate @00:03:08.99 Lights, camera, ACTION! @00:03:09.02 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23mar0800h650ml.tif @00:03:28.48 Waiting >20 seconds for da to relinquish FlushPuck @00:03:49.82 SX MX5/16 (USB:23): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 37.02 seconds /var/log/esp/da23mar0800h650ml.tif: TIFF Upload Complete @00:03:49.85 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23mar0800h650ml15s.tif @00:04:05.57 SX MX5/16 (USB:23): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23mar0800h650ml15s.tif: TIFF Upload Complete @00:04:05.59 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da23mar0800h650ml25s.tif @00:04:31.37 SX MX5/16 (USB:23): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da23mar0800h650ml25s.tif: TIFF Upload Complete @00:04:31.38 Gate.power :camera,:OFF @00:04:31.43 DAfans.email "37.02 sec auto-exposure",Subject: "da23mar0800h650ml images!" @00:04:31.70 Elbow.seek 2 @00:04:32.09 Forearm.seek 2 + 700 counts @00:04:32.16 SE.reconfigure :gently @00:04:32.22 SE.seek 16.55 Pucks below Top Plate @00:04:32.92 Elbow.reconfigure :limp @00:04:32.97 Forearm.reconfigure :loose @00:04:33.01 Forearm.seek 2 @00:04:33.40 Elbow.hold @00:04:33.47 Elbow.reconfigure :tight @00:04:33.52 SE.reconfigure :default @00:04:33.57 SE.seek 16.74 Pucks below Top Plate @00:04:33.91 Forearm.force -20 @00:04:33.94 Forearm.seek 2 + 303 counts @00:04:34.74 Forearm.reconfigure :tight @00:04:34.79 Forearm.seek 2 + 700 counts @00:04:35.32 SE.seek :down @00:04:36.60 Forearm.reconfigure :loose @00:04:36.68 Forearm.seek :retract @00:04:36.96 SE.stop @00:04:37.91 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @00:04:38.51 Elbow.seek FlushPuck::Garage @00:04:40.40 Elbow.reconfigure :loose @00:04:40.62 Forearm.seek :garage @00:04:41.80 Hand.close @00:04:42.72 Forearm.seek :retract @00:04:43.81 Elbow.reconfigure :tight @00:04:43.91 Elbow.seek PC @00:04:45.36 Elbow.reconfigure :loose @00:04:45.56 Forearm.reconfigure :tight @00:04:45.65 Forearm.seek PC @00:04:47.39 PC.seek :closed @00:04:51.40 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.42 @00:04:51.58 Hand.open @00:04:52.46 Forearm.reconfigure :loose @00:04:52.56 Forearm.seek :retract @00:04:53.64 PSR.select :air @00:04:53.70 Storing wcr puck in Collection Clamp to tube 2 @00:04:53.74 PSV.dial PSR @00:04:54.68 Pulling 0.5ml of PSR.flush @00:04:54.75 PSR.select :flush @00:04:54.88 Elbow.reconfigure :tight @00:04:54.96 Elbow.seek 2 @00:04:55.08 PS.seek 0.5ml @00:04:57.85 Forearm.reconfigure :tight @00:04:57.94 Forearm.seek 2 + 700 counts @00:04:59.14 SE.reconfigure :pick @00:04:59.26 SE.seek :beamBreak @00:05:01.03 delay 5 seconds @00:05:01.40 SE.configure :default @00:05:01.70 Elbow.reconfigure :limp @00:05:01.79 Forearm.reconfigure :loose @00:05:01.87 Forearm.seek 2 @00:05:02.34 Elbow.hold @00:05:02.47 Elbow.reconfigure :tight @00:05:02.64 SE.seek 17.3 Pucks below Top Plate @00:05:03.34 Forearm.force -20 @00:05:03.42 Forearm.seek 2 + 307 counts @00:05:03.89 Forearm.reconfigure :tight @00:05:03.97 Forearm.seek 2 + 700 counts @00:05:04.28 SE.seek 18.3 Pucks below Top Plate @00:05:05.12 Forearm.reconfigure :loose @00:05:05.20 Forearm.seek :retract @00:05:06.06 PSR.select :air @00:05:06.17 PS.seek 2.5ml @00:05:06.26 Elbow.seek CC @00:05:06.31 upsync @00:05:07.86 Elbow.reconfigure :loose @00:05:07.95 Forearm.seek CC @00:05:08.91 Hand.close @00:05:09.56 CC.seek :open @00:05:14.51 Forearm.seek :retract @00:05:15.51 Elbow.reconfigure :tight @00:05:15.56 Elbow.seek 2 @00:05:17.54 Elbow.reconfigure :limp @00:05:17.59 Forearm.seek 2 @00:05:18.74 Elbow.hold @00:05:18.81 Elbow.reconfigure :tight @00:05:18.85 Hand.open @00:05:19.68 Forearm.force -20 @00:05:19.71 Forearm.seek 2 + 315 counts @00:05:20.06 Forearm.reconfigure :tight @00:05:20.11 Forearm.seek 2 + 700 counts @00:05:20.40 SE.seek :down @00:05:21.53 Forearm.reconfigure :loose @00:05:21.58 Forearm.seek :retract @00:05:21.89 SE.stop @00:05:22.56
Starting finalPurge @00:05:27.28 delay 1 second @00:05:28.30 PRV.dial :waste2,avoiding: :ab2 @00:05:30.09 PTV.dial :PRVmixing @00:05:31.34 PSV.dial :mixloop @00:05:32.33 PS.seek :empty @00:05:41.94
Waiting >20 seconds for psFlush to relinquish FlushPuck @00:05:58.59 PSR.select :air @00:05:58.61 PSV.dial PSR @00:05:59.77 Pulling 0.5ml of PSR.flush @00:05:59.78 PSR.select :flush @00:05:59.89 PS.seek 0.5ml @00:06:05.78 delay 5 seconds @00:06:10.80 PSR.select :air @00:06:10.91 PS.seek 2.5ml @00:06:32.08 delay 1 second @00:06:33.09 PBV.dial :waste1 @00:06:33.45 PTV.dial :mixing @00:06:34.15 PSV.dial :mixloop @00:06:35.12 PS.seek :empty @00:07:01.27 PSV.dial PSR @00:07:02.26 PSR.select :flush @00:07:02.37 PS.seek 1ml @00:07:13.33 PSR.select :air @00:07:13.45 PS.seek 2.5ml @00:07:29.46 PSV.dial PTV @00:07:30.18 PTV.dial :puck @00:07:30.87 PBV.dial :waste1 @00:07:30.89 PS.seek 0ml @00:07:57.12 PSV.dial PSR @00:07:57.83 PSR.select :flush @00:07:57.95 PS.seek 0.5ml @00:08:03.90 PSR.select :air @00:08:04.01 PS.seek 2.5ml @00:08:25.18 PSV.dial PTV @00:08:25.90 PBV.dial PSV @00:08:26.44 PS.seek 0ml @00:08:52.71 PSV.dial PBV @00:08:53.17 PS.seek 2.5ml @00:09:19.33 PSV.dial :waste1 @00:09:20.04 PS.seek 0ml @00:09:46.21 PSV.dial PSR @00:09:46.64 PSR.select :air @00:09:46.67 PS.seek 2.5ml @00:10:13.02 PSV.dial PTV @00:10:13.85 PBV.dial PSV @00:10:13.87 PS.seek 0ml @00:10:40.02 PSV.dial PBV @00:10:40.46 PS.seek 2.5ml @00:11:06.67 PSV.dial :waste1 @00:11:07.37 PS.seek 0ml @00:11:33.48 PSV.dial PSR @00:11:33.92 PSR.select :flush @00:11:34.04 PS.seek 1ml @00:11:45.01 PSR.select :air @00:11:45.13 PS.seek 2.5ml @00:12:01.14 PSV.dial PTV @00:12:01.97 PTV.dial :puck @00:12:01.98 PBV.dial :waste1 @00:12:02.51 PS.seek 0ml @00:12:28.64 PSV.dial PSR @00:12:29.80 PSR.select :flush @00:12:29.91 PS.seek 0.5ml @00:12:35.76 PSR.select :air @00:12:35.88 PS.seek 2.5ml @00:12:57.01 PSV.dial PTV @00:12:57.73 PBV.dial PSV @00:12:58.27 PS.seek 0ml @00:13:24.39 PSV.dial PBV @00:13:24.85 PS.seek 2.5ml @00:13:51.32 PSV.dial :waste1 @00:13:52.04 PS.seek 0ml @00:14:18.17 PSV.dial PSR @00:14:18.61 PSR.select :air @00:14:18.63 PS.seek 2.5ml @00:14:44.82 PSV.dial PTV @00:14:45.53 PBV.dial PSV @00:14:45.55 PS.seek 0ml @00:15:11.79 PSV.dial PBV @00:15:12.24 PS.seek 2.5ml @00:15:38.45 PSV.dial :waste1 @00:15:39.16 PS.seek 0ml @00:16:05.39
Loading FlushPuck into Collection Clamp from Processing Clamp @00:16:05.56 Elbow.seek PC @00:16:08.12 Elbow.reconfigure :loose @00:16:08.21 Forearm.seek PC @00:16:09.27 Hand.close @00:16:09.91 PC.seek :open @00:16:13.87 Forearm.seek :retract @00:16:14.88 Elbow.reconfigure :tight @00:16:14.92 Elbow.seek CC @00:16:18.51 Elbow.reconfigure :loose @00:16:18.60 Forearm.reconfigure :tight @00:16:18.64 Forearm.seek CC @00:16:19.94 CC.seek :closed @00:16:24.17 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.76 @00:16:24.24 Hand.open @00:16:25.09 Forearm.reconfigure :loose @00:16:25.13 Forearm.seek :retract @00:16:26.13 Flushing the Collection Valve::Manifold @00:16:26.25 CSV.dial CSR @00:16:26.72 Pulling 5ml of CSR.flush @00:16:26.73 CSR.select :flush @00:16:26.84 CS.seek 5ml @00:16:40.24 delay 5 seconds @00:16:45.26 CSR.select :air @00:16:45.41 CS.reconfigure :fast @00:16:45.45 CS.seek 7.5ml @00:16:51.74 CSV.dial :waste @00:16:52.98 CS.reconfigure :default @00:16:53.03 CS.seek 0ml @00:17:12.77 Flushing lower manifold's waste1 port @00:17:12.89 CSV.dial :flush @00:17:13.32 CS.reconfigure :fast @00:17:13.37 CS.seek 5ml @00:17:25.02 delay 5 seconds @00:17:30.04 CSV.dial :air @00:17:30.54 CS.seek 7ml @00:17:35.72 CSV.dial CTV @00:17:35.74
CTV.dial CSV @00:17:37.05 CBV.dial :waste1 @00:17:37.78 CS.seek 0ml @00:17:53.68 Flushing lower manifold's waste2 port @00:17:53.79 CSV.dial :flush @00:17:54.50 CS.seek 5ml @00:18:06.11 delay 5 seconds @00:18:11.13 CSV.dial :air @00:18:11.64 CS.seek 7ml @00:18:16.93 CSV.dial CTV @00:18:16.95
CTV.dial CSV @00:18:17.37 CBV.dial :waste2 @00:18:18.37 CS.seek 0ml @00:18:34.37 Flushing lower manifold's waste2b port @00:18:34.48 CSV.dial :flush @00:18:35.20 CS.seek 5ml @00:18:46.78 delay 5 seconds @00:18:51.80 CSV.dial :air @00:18:52.32 CS.seek 6.999ml @00:18:57.50 CSV.dial CTV @00:18:57.52
CTV.dial CSV @00:18:57.94 CBV.dial :waste2b @00:18:58.95 CS.seek 0ml @00:19:14.74 Disinfecting external Intake with kill @00:19:14.85 CSV.dial CSR @00:19:15.34 Pulling 8ml of CSR.kill @00:19:15.35 CSR.select :kill @00:19:15.46 CS.seek 8ml @00:19:33.52 delay 5 seconds @00:19:38.54 CSR.select :air @00:19:38.65 CS.seek 10ml @00:19:43.83 CSV.dial CBV @00:19:43.86
CBV.dial CSV @00:19:44.86 CTV.dial :intake @00:19:45.56 Intake.select :open @00:19:45.67 CS.seek 2ml @00:20:03.64 Intake.select :close @00:20:03.74 CTV.dial :air @00:20:04.48 CS.reconfigure :default @00:20:04.52 CS.seek 0ml @00:20:10.39 CSV.dial CSR @00:20:11.42 Pulling 5ml of CSR.flush CSR.select :flush @00:20:11.53 CS.seek 5ml @00:20:24.99 delay 5 seconds @00:20:30.01 CSR.select :air @00:20:30.12 CS.seek 10ml @00:20:43.48 CSV.dial CBV @00:20:44.46 CS.seek 0ml @00:21:10.52 Garaging FlushPuck in Collection Clamp @00:21:10.59 Elbow.reconfigure :tight @00:21:10.63 Elbow.seek CC @00:21:10.73 Elbow.reconfigure :loose @00:21:10.82 Forearm.seek CC @00:21:11.77 Hand.close @00:21:12.40 CC.seek :open @00:21:16.72 Forearm.seek :retract @00:21:17.71 Elbow.reconfigure :tight @00:21:17.75 Elbow.seek :garage @00:21:20.62 Elbow.reconfigure :loose @00:21:20.71 Forearm.reconfigure :tight @00:21:20.75 Forearm.seek :garage @00:21:21.90 Hand.open @00:21:22.71 Forearm.reconfigure :loose @00:21:22.75 Forearm.seek :retract @00:21:23.87 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 22:04:22 @00:21:23.91 Asleep after completing DA_WCR_030623 assays @00:21:23.93 GoodNews.email "{1 => 33,\n 2 => 19,\n 3 => 14,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@00:13:27, 22.9C, 53% humidity, 15.6psia, 14.060V, 0.282A, 0.282A avg, 3.96W\nSampled 175.0ml",Subject: "Asleep after completing DA_WCR_030623 assays" @00:21:29.16 upsync @19:26:46.80UTC08-Mar-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @19:26:49.36 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @19:26:53.31 Polling Can every 10:00 @19:26:53.40 Cleanly exited at 19:18:18UTC08-Mar-23 @19:26:53.44 Loading /home/esp/esp2/mission/DA_WCR_030823.rb mission @19:26:53.49 GoodNews.email "Starting DA_WCR_030823 w/pucks in tube 3\n{1 => 33,\n 2 => 19,\n 3 => 14,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:26:53, 17.8C, 49% humidity, 15.5psia, 13.992V, 0.251A, 0.251A avg, 3.51W",Subject: "DA_WCR_030823 begun" @19:26:53.53
Preparing to sleep until + 00:00:05 @19:26:53.56 Asleep til + 00:00:05 @19:26:53.58 Continuing at 19:26 @19:26:53.86 Gate.power :core,:OFF @19:26:53.88 Gate.power :raw,:OFF Shutdown @19:26:56.66 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_030823.rb:14\n{1 => 33,\n 2 => 19,\n 3 => 14,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:26:53, 17.8C, 49% humidity, 15.5psia, 13.992V, 0.251A, 0.251A avg, 3.51W",Subject: "Asleep until 19:26" @19:26:58.28 upsync @19:26:58.48
Powering core bus @19:27:00.99 upsync @19:27:04.19
Gate.power :raw,:ON @19:27:05.04 CS.configure :default @19:27:05.13 PS.configure :default @19:27:05.22 SS.configure :default @19:27:05.30 CC.configure :default @19:27:05.39 PC.configure :default @19:27:05.50 Forearm.configure :loose @19:27:05.58 Elbow.configure :loose @19:27:05.69 SC.configure :loose @19:27:05.77 SE.configure :default @19:27:05.81 CH.coast @19:27:05.87 CH.configure :default @19:27:05.91 PH.coast @19:27:05.96 PH.configure :default @19:27:06.00 All dwarf controllers running firmware version 3.85 @19:27:06.10 Valve.reset @19:27:06.33 Intake.select :close @19:27:06.36 Exhaust.select :close @19:27:06.39 CTV.dial :air @19:27:06.47 PSV.dial :air @19:27:06.52 CSV.dial :waste1 @19:27:06.65 PS.reconfigure :default @19:27:06.67
Forearm.reconfigure :loose @19:27:06.75 PS.home.seek 0ml @19:27:06.78
Forearm.home.seek :retract @19:27:07.01 SSV.dial :spit,avoiding: :exhaust @19:27:07.15 CS.reconfigure :fast @19:27:07.18 SS.reconfigure :default @19:27:07.26 CS.home.seek 0ml @19:27:07.29 SS.home.seek 0ml @19:27:07.57 Hand.close @19:27:07.62
Elbow.jog 1500 @19:27:08.05 Elbow.reconfigure :loose @19:27:08.10 Elbow.home.seek :clear @19:27:11.31 SE.reconfigure :default @19:27:11.36 SE.home.seek :down @19:27:11.95 SC.reconfigure :tight @19:27:12.00 SC.home.seek :initial @19:27:15.41 PS.stop @19:27:42.05 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @19:27:42.06
Retry #1 of 3 @19:27:42.12 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @19:27:43.34 SC.configure :limp @19:27:43.39 Forearm.seek :retract @19:27:43.45 Elbow.seek :hammer @19:27:44.54 Forearm.reconfigure :tight @19:27:44.58 Forearm.seek retracted @19:27:44.95 PC.reconfigure :default @19:27:44.97
CC.reconfigure :default @19:27:45.04 PC.home.seek :open @19:27:45.07
CC.home.seek :open @19:27:48.31 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @19:27:48.32 Notice.email "At 19:27:48 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_030823.rb:14\n{1 => 33,\n 2 => 19,\n 3 => 14,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:27:48, 17.8C, 49% humidity, 15.5psia, 13.992V, 0.245A, 0.245A avg, 3.43W",Subject: "Started DA_WCR_030823 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @19:27:48.34
Starting initialPurge @19:27:48.37 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:27:48.43 Hand.open @19:27:49.31 Elbow.reconfigure :tight @19:27:49.37 Elbow.seek FlushPuck::Garage @19:27:51.36 Elbow.reconfigure :loose @19:27:51.54 Forearm.reconfigure :loose @19:27:51.58 Forearm.seek :garage @19:27:52.57 upsync @19:27:52.69
Hand.close @19:27:53.56 Forearm.seek :retract @19:27:54.59 Elbow.reconfigure :tight @19:27:54.63 Elbow.seek CC @19:27:57.54 Elbow.reconfigure :loose @19:27:57.65 Forearm.reconfigure :tight @19:27:57.70 Forearm.seek CC @19:27:58.95 CC.seek :closed @19:28:03.18 Collection Clamp onSpring/offSpring for 8/25 samples, on/off torque 1.85 @19:28:03.25 Hand.open @19:28:04.00 Forearm.reconfigure :loose @19:28:04.05 Forearm.seek :retract @19:28:05.07 Flushing the Collection Valve::Manifold @19:28:05.19 CSV.dial CSR @19:28:06.50 Pulling 5ml of CSR.flush @19:28:06.51 CSR.select :flush @19:28:06.62 CS.reconfigure :default @19:28:06.66 CS.seek 5ml @19:28:20.07 delay 5 seconds @19:28:25.09 CSR.select :air @19:28:25.24 CS.reconfigure :fast @19:28:25.29 CS.seek 7.501ml @19:28:31.75 CSV.dial :waste @19:28:33.14 CS.reconfigure :default @19:28:33.18 CS.seek 0ml @19:28:52.88 Purging external Intake @19:28:53.00 CSV.dial :flush @19:28:53.46 CS.reconfigure :fast @19:28:53.51 CS.seek 10ml @19:29:16.38 delay 5 seconds @19:29:21.40 CSV.dial CBV @19:29:21.43
CBV.dial CSV @19:29:22.18 CTV.dial :intake @19:29:22.93 Intake.select :open @19:29:23.03 CS.seek 0ml @19:29:45.72 Intake.select :close @19:29:45.82 CTV.dial :air @19:29:46.57 Flushing upper manifold @19:29:46.68 CSV.dial :flush @19:29:47.42 CS.seek 5ml @19:29:59.26 delay 5 seconds @19:30:04.27 CSV.dial :air @19:30:04.77 CS.seek 7ml @19:30:09.98 CSV.dial CBV @19:30:10.01
CBV.dial CSV @19:30:11.02 CTV.dial :air @19:30:11.04 CS.seek 0ml @19:30:27.19 delay 7 seconds @19:30:34.22 CS.seek 3ml @19:30:41.69 CSV.dial :waste @19:30:42.14 CS.seek 0ml @19:30:49.58 CSV.dial :flush @19:30:50.01 CS.seek 3ml @19:30:57.51 delay 5 seconds @19:31:02.52 CSV.dial :air @19:31:03.14 CS.seek 5ml @19:31:08.38 CSV.dial :air @19:31:08.40 CS.seek 0ml @19:31:20.13 delay 7 seconds @19:31:27.16 CS.seek 3ml @19:31:34.69 CSV.dial :waste @19:31:35.40 CS.seek 0ml @19:31:42.88 Garaging FlushPuck in Collection Clamp @19:31:42.95 Elbow.reconfigure :tight @19:31:42.99 Elbow.seek CC @19:31:43.10 Elbow.reconfigure :loose @19:31:43.19 Forearm.seek CC @19:31:44.14 Hand.close @19:31:44.81 CC.seek :open @19:31:49.18 Forearm.seek :retract @19:31:50.14 Elbow.reconfigure :tight @19:31:50.18 Elbow.seek :garage @19:31:53.07 Elbow.reconfigure :loose @19:31:53.15 Forearm.reconfigure :tight @19:31:53.20 Forearm.seek :garage @19:31:54.36 Hand.open @19:31:55.48 Forearm.reconfigure :loose @19:31:55.60 Forearm.seek :retract @19:31:56.64 da sampling at most 1000ml, wcr at most 1000ml @19:31:56.67 DAfans.email "Started da in tube 3 at 19:31:56UTC08-Mar-23\n{1 => 33,\n 2 => 19,\n 3 => 14,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@19:27:48, 17.8C, 49% humidity, 15.5psia, 13.992V, 0.245A, 0.245A avg, 3.43W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @19:31:56.69
Loading sh1 puck from tube 3 into Collection Clamp @19:31:56.94 SC.reconfigure :tight @19:31:57.01 SC.seek 3 @19:32:00.73 upsync @19:32:06.86
SC.configure :limp @19:32:07.06 Elbow.reconfigure :tight @19:32:07.11 Elbow.seek 3 @19:32:09.09 Forearm.reconfigure :tight @19:32:09.14 Forearm.seek 3 + 700 counts @19:32:10.19 SE.reconfigure :pick @19:32:10.24 SE.seek :beamBreak @19:32:12.52 SE.configure :default @19:32:12.67 Elbow.seek 3 @19:32:12.84 Forearm.seek 3 + 300 counts @19:32:13.01 Forearm.reconfigure :loose @19:32:13.05 Hand.close @19:32:13.82 SE.seek :down @19:32:14.01
Forearm.seek :retract @19:32:15.05 Elbow.seek CC @19:32:15.77 SE.stop @19:32:16.60
Elbow.reconfigure :loose @19:32:16.69 Forearm.reconfigure :tight @19:32:16.73 Forearm.seek CC @19:32:18.02 CC.seek :closed @19:32:22.26 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.70 @19:32:22.27 Hand.open @19:32:23.13 Forearm.reconfigure :loose @19:32:23.18 Forearm.seek :retract @19:32:24.22 collectSample 1000ml w/bubble point of 24psi @19:32:24.23 Priming 7ml @19:32:24.24 Intake.select :close @19:32:24.25 Exhaust.select :close @19:32:24.26 SS.hold @19:32:24.45 Calibrating pressure and temperature sensors @19:32:24.48 CTV.dial :air @19:32:24.50 SSV.dial :spit,avoiding: :exhaust @19:32:24.51 SS.reconfigure :fast @19:32:24.56 SS.seek 5ml @19:32:27.42 SSV.dial CBV @19:32:27.45
CBV.dial SSV @19:32:28.78 delay 3.8 seconds @19:32:32.69 SSV.dial :spit @19:32:33.99 SS.seek 0ml @19:32:36.98 SSV.dial CBV @19:32:38.28 CTV.dial :intake @19:32:39.01 SSV.dial CBV @19:32:39.04
CBV.dial SSV @19:32:39.05 Allowing 40s for 7.0ml of remaining 7.0ml @19:32:39.06 Intake.select :open @19:32:41.22 Intake @15.1psia, vacuum from 9.6 to 12.6psi @19:32:41.23 SS.reconfigure :inhaling 2.14ml/s @19:32:41.27 SS.seek 7ml @19:32:47.01 Stroke completed -- waiting for pressure to equalize ... @19:32:47.02 SS.reconfigure :exhale @19:32:47.06 SS.hold @19:32:47.36 Intake.select :close @19:32:47.50 SSV.dial :exhaust @19:32:48.25 Exhaust.select :open @19:32:48.36 SS.seek 0ml @19:32:52.72 Exhaust.select :close @19:32:52.82 Sampling 1000.0ml @19:32:52.83 Intake.select :close @19:32:52.84 Exhaust.select :close @19:32:52.85 SS.reconfigure :default @19:32:52.89 SS.hold @19:32:53.07 CTV.dial :intake @19:32:53.08 SSV.dial CBV @19:32:53.10
CBV.dial SSV @19:32:53.86 Allowing 130s for 25.0ml of remaining 1000.0ml @19:32:53.87 Intake.select :open @19:32:56.02 Intake @15.1psia, vacuum from 9.6 to 12.6psi @19:32:56.04 SS.reconfigure :inhaling 2.14ml/s @19:32:56.08 SS.seek 25ml @19:33:00.62 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @19:33:00.66 SS.hold @19:33:01.35 Intake @12.7psia, vacuum from 7.2 to 10.2psi @19:33:01.37 SS.reconfigure :inhaling 1.93ml/s @19:33:01.41 SS.seek 25ml @19:33:03.71 Vacuum too high -- waiting up to 40 seconds @19:33:03.72 SS.reconfigure :exhale @19:33:03.76 SS.hold @19:33:04.67 Intake @16.7psia, vacuum from 10.0 to 13.0psi @19:33:04.68 SS.reconfigure :inhaling 1.74ml/s @19:33:04.73 SS.seek 25ml @19:33:07.30 Vacuum too high -- waiting up to 40 seconds @19:33:07.76 SS.reconfigure :exhale @19:33:07.81 SS.hold @19:33:08.03 Intake @16.5psia, vacuum from 10.0 to 13.0psi @19:33:08.05 SS.reconfigure :inhaling 1.57ml/s @19:33:08.09 SS.seek 25ml @19:33:10.21 Vacuum too high -- waiting up to 40 seconds @19:33:10.22 SS.reconfigure :exhale @19:33:10.26 SS.hold @19:33:10.84 Intake @15.4psia, vacuum from 9.9 to 12.9psi @19:33:10.86 SS.reconfigure :inhaling 1.41ml/s @19:33:10.91 SS.seek 25ml @19:33:13.90 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @19:33:13.94 SS.hold @19:33:14.46 Intake @15.3psia, vacuum from 9.8 to 12.8psi @19:33:14.48 SS.reconfigure :inhaling 1.27ml/s @19:33:14.52 SS.seek 25ml @19:33:18.22 Vacuum too high -- waiting up to 40 seconds @19:33:18.23 SS.reconfigure :exhale @19:33:18.27 SS.hold @19:33:18.95 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:33:18.97 SS.reconfigure :inhaling 1.14ml/s @19:33:19.01 SS.seek 25ml @19:33:23.18 Vacuum too high -- waiting up to 40 seconds @19:33:23.19 SS.reconfigure :exhale @19:33:23.23 SS.hold @19:33:23.64 Intake @15.0psia, vacuum from 9.5 to 12.5psi @19:33:23.65 SS.reconfigure :inhaling 1.03ml/s @19:33:23.69 SS.seek 25ml @19:33:25.92 Stroke completed -- waiting for pressure to equalize ... @19:33:25.93 SS.reconfigure :exhale @19:33:25.97 SS.hold @19:33:26.62 Intake.select :close @19:33:26.76 SSV.dial :exhaust @19:33:27.48 Exhaust.select :open @19:33:27.59 SS.seek 0ml @19:33:36.37 Exhaust.select :close @19:33:36.48 SSV.dial CBV @19:33:36.50
CBV.dial SSV @19:33:37.25 Allowing 130s for 25.0ml of remaining 975.0ml @19:33:37.26 Intake.select :open @19:33:39.41 Intake @14.7psia, vacuum from 9.2 to 12.2psi @19:33:39.43 SS.reconfigure :inhaling 1.03ml/s @19:33:39.47 SS.seek 25ml @19:34:06.23 Stroke completed -- waiting for pressure to equalize ... @19:34:06.24 SS.reconfigure :exhale @19:34:06.28 SS.hold @19:34:07.47 Intake.select :close @19:34:07.61 SSV.dial :exhaust @19:34:08.36 Exhaust.select :open @19:34:08.46 SS.seek 0ml @19:34:17.24 Exhaust.select :close @19:34:17.35 SSV.dial CBV @19:34:17.37
CBV.dial SSV @19:34:18.12 Allowing 130s for 25.0ml of remaining 950.0ml @19:34:18.13 Intake.select :open @19:34:20.28 Intake @14.5psia, vacuum from 9.0 to 12.0psi @19:34:20.30 SS.reconfigure :inhaling 1.03ml/s @19:34:20.34 SS.seek 25ml @19:34:47.09 Stroke completed -- waiting for pressure to equalize ... @19:34:47.10 SS.reconfigure :exhale @19:34:47.14 SS.hold @19:34:48.00 Intake.select :close @19:34:48.14 SSV.dial :exhaust @19:34:48.87 Exhaust.select :open @19:34:48.98 SS.seek 0ml @19:34:58.04 Exhaust.select :close @19:34:58.14 SSV.dial CBV @19:34:58.18
CBV.dial SSV @19:34:58.92 Allowing 130s for 25.0ml of remaining 925.0ml @19:34:58.93 Intake.select :open @19:35:01.08 Intake @14.3psia, vacuum from 8.8 to 11.8psi @19:35:01.10 SS.reconfigure :inhaling 1.03ml/s @19:35:01.14 SS.seek 25ml @19:35:21.92 Vacuum too high -- waiting up to 40 seconds @19:35:21.93 SS.reconfigure :exhale @19:35:21.97 SS.hold @19:35:22.36 Intake @12.7psia, vacuum from 7.2 to 10.2psi @19:35:22.38 SS.reconfigure :inhaling 0.93ml/s @19:35:22.42 SS.seek 25ml @19:35:28.16 Stroke completed -- waiting for pressure to equalize ... @19:35:28.17 SS.reconfigure :exhale @19:35:28.21 SS.hold @19:35:29.07 Intake.select :close @19:35:29.21 SSV.dial :exhaust @19:35:29.95 Exhaust.select :open @19:35:30.06 SS.seek 0ml @19:35:38.80 Exhaust.select :close @19:35:38.91 SSV.dial CBV @19:35:38.93
CBV.dial SSV @19:35:39.68 Allowing 130s for 25.0ml of remaining 900.0ml @19:35:39.69 Intake.select :open @19:35:41.84 Intake @14.2psia, vacuum from 8.7 to 11.7psi @19:35:41.86 SS.reconfigure :inhaling 0.93ml/s @19:35:41.90 SS.seek 25ml @19:36:11.18 Stroke completed -- waiting for pressure to equalize ... @19:36:11.19 SS.reconfigure :exhale @19:36:11.23 SS.hold @19:36:11.79 Intake.select :close @19:36:11.93 SSV.dial :exhaust @19:36:13.07 Exhaust.select :open @19:36:13.18 SS.seek 0ml @19:36:21.90 Exhaust.select :close @19:36:22.00 SSV.dial CBV @19:36:22.04
CBV.dial SSV @19:36:22.76 Allowing 130s for 25.0ml of remaining 875.0ml @19:36:22.77 Intake.select :open @19:36:24.94 Intake @14.2psia, vacuum from 8.7 to 11.7psi @19:36:24.95 SS.reconfigure :inhaling 0.93ml/s @19:36:24.99 SS.seek 25ml @19:36:54.24 Stroke completed -- waiting for pressure to equalize ... @19:36:54.25 SS.reconfigure :exhale @19:36:54.29 SS.hold @19:36:55.24 Intake.select :close @19:36:55.39 SSV.dial :exhaust @19:36:56.13 Exhaust.select :open @19:36:56.24 SS.seek 0ml @19:37:04.99 Exhaust.select :close @19:37:05.09 SSV.dial CBV @19:37:05.12
CBV.dial SSV @19:37:05.87 Allowing 130s for 25.0ml of remaining 850.0ml @19:37:05.88 Intake.select :open @19:37:08.03 Intake @14.1psia, vacuum from 8.6 to 11.6psi @19:37:08.05 SS.reconfigure :inhaling 0.93ml/s @19:37:08.09 SS.seek 25ml @19:37:37.24 Stroke completed -- waiting for pressure to equalize ... @19:37:37.25 SS.reconfigure :exhale @19:37:37.29 SS.hold @19:37:38.23 Intake.select :close @19:37:38.38 SSV.dial :exhaust @19:37:39.55 Exhaust.select :open @19:37:39.67 SS.seek 0ml @19:37:48.89 Exhaust.select :close @19:37:49.00 SSV.dial CBV @19:37:49.03
CBV.dial SSV @19:37:49.77 Allowing 130s for 25.0ml of remaining 825.0ml @19:37:49.78 Intake.select :open @19:37:51.94 Intake @14.2psia, vacuum from 8.7 to 11.7psi @19:37:51.95 SS.reconfigure :inhaling 0.93ml/s @19:37:51.99 SS.seek 25ml @19:38:20.47 Stroke completed -- waiting for pressure to equalize ... @19:38:20.48 SS.reconfigure :exhale @19:38:20.52 SS.hold @19:38:21.18 Intake.select :close @19:38:21.32 SSV.dial :exhaust @19:38:22.07 Exhaust.select :open @19:38:22.18 SS.seek 0ml @19:38:30.92 Exhaust.select :close @19:38:31.03 SSV.dial CBV @19:38:31.05
CBV.dial SSV @19:38:31.80 Allowing 130s for 25.0ml of remaining 800.0ml @19:38:31.81 Intake.select :open @19:38:33.97 Intake @14.2psia, vacuum from 8.7 to 11.7psi @19:38:33.98 SS.reconfigure :inhaling 0.93ml/s @19:38:34.03 SS.seek 25ml @19:39:03.25 Stroke completed -- waiting for pressure to equalize ... @19:39:03.26 SS.reconfigure :exhale @19:39:03.30 SS.hold @19:39:04.16 Intake.select :close @19:39:04.30 SSV.dial :exhaust @19:39:05.02 Exhaust.select :open @19:39:05.13 SS.seek 0ml @19:39:13.91 Exhaust.select :close @19:39:14.01 SSV.dial CBV @19:39:14.05
CBV.dial SSV @19:39:14.77 Allowing 130s for 25.0ml of remaining 775.0ml @19:39:14.78 Intake.select :open @19:39:16.94 Intake @14.1psia, vacuum from 8.6 to 11.6psi @19:39:16.95 SS.reconfigure :inhaling 0.93ml/s @19:39:16.99 SS.seek 25ml @19:39:46.16 Stroke completed -- waiting for pressure to equalize ... @19:39:46.17 SS.reconfigure :exhale @19:39:46.20 SS.hold @19:39:47.40 Intake.select :close @19:39:47.54 SSV.dial :exhaust @19:39:48.28 Exhaust.select :open @19:39:48.39 SS.seek 0ml @19:39:57.14 Exhaust.select :close @19:39:57.24 SSV.dial CBV @19:39:57.28
CBV.dial SSV @19:39:57.99 Allowing 130s for 25.0ml of remaining 750.0ml @19:39:58.00 Intake.select :open @19:40:00.15 Intake @14.1psia, vacuum from 8.6 to 11.6psi @19:40:00.16 SS.reconfigure :inhaling 0.93ml/s @19:40:00.21 SS.seek 25ml @19:40:29.37 Stroke completed -- waiting for pressure to equalize ... @19:40:29.38 SS.reconfigure :exhale @19:40:29.43 SS.hold @19:40:30.08 Intake.select :close @19:40:30.22 SSV.dial :exhaust @19:40:30.95 Exhaust.select :open @19:40:31.06 SS.seek 0ml @19:40:39.89 Exhaust.select :close @19:40:39.99 SSV.dial CBV @19:40:40.03
CBV.dial SSV @19:40:40.77 Allowing 130s for 25.0ml of remaining 725.0ml @19:40:40.78 Intake.select :open @19:40:42.93 Intake @14.2psia, vacuum from 8.7 to 11.7psi @19:40:42.95 SS.reconfigure :inhaling 0.93ml/s @19:40:42.99 SS.seek 25ml @19:41:12.22 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @19:41:12.26 SS.hold @19:41:13.12 Intake.select :close @19:41:13.26 SSV.dial :exhaust @19:41:14.00 Exhaust.select :open @19:41:14.11 SS.seek 0ml @19:41:23.00 Exhaust.select :close @19:41:23.10 SSV.dial CBV @19:41:23.15
CBV.dial SSV @19:41:23.86 Allowing 130s for 25.0ml of remaining 700.0ml @19:41:23.87 Intake.select :open @19:41:26.02 Intake @14.1psia, vacuum from 8.6 to 11.6psi @19:41:26.04 SS.reconfigure :inhaling 0.93ml/s @19:41:26.08 SS.seek 25ml @19:41:55.35 Stroke completed -- waiting for pressure to equalize ... @19:41:55.36 SS.reconfigure :exhale @19:41:55.41 SS.hold @19:41:56.59 Intake.select :close @19:41:56.73 SSV.dial :exhaust @19:41:57.47 Exhaust.select :open @19:41:57.58 SS.seek 0ml @19:42:06.82 Exhaust.select :close @19:42:06.93 SSV.dial CBV @19:42:06.95
CBV.dial SSV @19:42:07.70 Allowing 130s for 25.0ml of remaining 675.0ml @19:42:07.71 Intake.select :open @19:42:09.86 Intake @14.1psia, vacuum from 8.6 to 11.6psi @19:42:09.88 SS.reconfigure :inhaling 0.93ml/s @19:42:09.92 SS.seek 25ml @19:42:38.37 Stroke completed -- waiting for pressure to equalize ... @19:42:38.38 SS.reconfigure :exhale @19:42:38.42 SS.hold @19:42:39.27 Intake.select :close @19:42:39.41 SSV.dial :exhaust @19:42:40.15 Exhaust.select :open @19:42:40.26 SS.seek 0ml @19:42:49.59 Exhaust.select :close @19:42:49.69 SSV.dial CBV @19:42:49.74
CBV.dial SSV @19:42:50.45 Allowing 130s for 25.0ml of remaining 650.0ml @19:42:50.46 Intake.select :open @19:42:52.62 Intake @14.2psia, vacuum from 8.7 to 11.7psi @19:42:52.63 SS.reconfigure :inhaling 0.93ml/s @19:42:52.67 SS.seek 25ml @19:43:21.16 Stroke completed -- waiting for pressure to equalize ... @19:43:21.17 SS.reconfigure :exhale @19:43:21.21 SS.hold @19:43:22.40 Intake.select :close @19:43:22.54 SSV.dial :exhaust @19:43:23.26 Exhaust.select :open @19:43:23.37 SS.seek 0ml @19:43:32.16 Exhaust.select :close @19:43:32.27 SSV.dial CBV @19:43:32.29
CBV.dial SSV @19:43:33.03 Allowing 130s for 25.0ml of remaining 625.0ml @19:43:33.04 Intake.select :open @19:43:35.19 Intake @14.2psia, vacuum from 8.7 to 11.7psi @19:43:35.21 SS.reconfigure :inhaling 0.93ml/s @19:43:35.25 SS.seek 25ml @19:44:04.51 Stroke completed -- waiting for pressure to equalize ... @19:44:04.52 SS.reconfigure :exhale @19:44:04.93 SS.hold @19:44:05.80 Intake.select :close @19:44:05.94 SSV.dial :exhaust @19:44:06.68 Exhaust.select :open @19:44:06.79 SS.seek 0ml @19:44:15.94 Exhaust.select :close @19:44:16.05 SSV.dial CBV @19:44:16.08
CBV.dial SSV @19:44:16.81 Allowing 130s for 25.0ml of remaining 600.0ml @19:44:16.82 Intake.select :open @19:44:18.97 Intake @14.2psia, vacuum from 8.7 to 11.7psi @19:44:18.99 SS.reconfigure :inhaling 0.93ml/s @19:44:19.03 SS.seek 25ml @19:44:47.27 Stroke completed -- waiting for pressure to equalize ... @19:44:47.28 SS.reconfigure :exhale @19:44:47.32 SS.hold @19:44:48.61 Intake.select :close @19:44:48.75 SSV.dial :exhaust @19:44:49.51 Exhaust.select :open @19:44:49.62 SS.seek 0ml @19:44:58.97 Exhaust.select :close @19:44:59.08 SSV.dial CBV @19:44:59.11
CBV.dial SSV @19:44:59.85 Allowing 130s for 25.0ml of remaining 575.0ml @19:44:59.86 Intake.select :open @19:45:02.02 Intake @14.3psia, vacuum from 8.8 to 11.8psi @19:45:02.03 SS.reconfigure :inhaling 0.93ml/s @19:45:02.08 SS.seek 25ml @19:45:30.44 Stroke completed -- waiting for pressure to equalize ... @19:45:30.45 SS.reconfigure :exhale @19:45:30.49 SS.hold @19:45:32.04 Intake.select :close @19:45:32.18 SSV.dial :exhaust @19:45:32.91 Exhaust.select :open @19:45:33.02 SS.seek 0ml @19:45:42.25 Exhaust.select :close @19:45:42.36 SSV.dial CBV @19:45:42.39
CBV.dial SSV @19:45:43.13 Allowing 130s for 25.0ml of remaining 550.0ml @19:45:43.14 Intake.select :open @19:45:45.29 Intake @14.3psia, vacuum from 8.8 to 11.8psi @19:45:45.31 SS.reconfigure :inhaling 0.93ml/s @19:45:45.35 SS.seek 25ml @19:46:14.69 Stroke completed -- waiting for pressure to equalize ... @19:46:14.70 SS.reconfigure :exhale @19:46:14.74 SS.hold @19:46:16.29 Intake.select :close @19:46:16.43 SSV.dial :exhaust @19:46:17.18 Exhaust.select :open @19:46:17.28 SS.seek 0ml @19:46:26.17 Exhaust.select :close @19:46:26.28 SSV.dial CBV @19:46:26.30
CBV.dial SSV @19:46:27.05 Allowing 130s for 25.0ml of remaining 525.0ml @19:46:27.06 Intake.select :open @19:46:29.22 Intake @14.3psia, vacuum from 8.8 to 11.8psi @19:46:29.23 SS.reconfigure :inhaling 0.93ml/s @19:46:29.27 SS.seek 25ml @19:46:58.56 Stroke completed -- waiting for pressure to equalize ... @19:46:58.57 SS.reconfigure :exhale @19:46:58.61 SS.hold @19:47:00.56 Intake.select :close @19:47:00.70 SSV.dial :exhaust @19:47:01.43 Exhaust.select :open @19:47:01.54 SS.seek 0ml @19:47:10.47 Exhaust.select :close @19:47:10.57 SSV.dial CBV @19:47:10.61
CBV.dial SSV @19:47:11.36 Allowing 130s for 25.0ml of remaining 500.0ml @19:47:11.37 Intake.select :open @19:47:13.52 Intake @14.4psia, vacuum from 8.9 to 11.9psi @19:47:13.54 SS.reconfigure :inhaling 0.93ml/s @19:47:13.58 SS.seek 25ml @19:47:42.91 Stroke completed -- waiting for pressure to equalize ... @19:47:42.92 SS.reconfigure :exhale @19:47:42.96 SS.hold @19:47:44.91 Intake.select :close @19:47:45.05 SSV.dial :exhaust @19:47:45.78 Exhaust.select :open @19:47:45.89 SS.seek 0ml @19:47:54.84 Exhaust.select :close @19:47:54.95 SSV.dial CBV @19:47:54.97
CBV.dial SSV @19:47:55.72 Allowing 130s for 25.0ml of remaining 475.0ml @19:47:55.73 Intake.select :open @19:47:57.89 Intake @14.4psia, vacuum from 8.9 to 11.9psi @19:47:57.90 SS.reconfigure :inhaling 0.93ml/s @19:47:57.94 SS.seek 25ml @19:48:24.68 Vacuum too high -- waiting up to 40 seconds @19:48:24.69 SS.reconfigure :exhale @19:48:25.18 SS.hold @19:48:25.57 Intake @12.2psia, vacuum from 6.7 to 9.7psi @19:48:25.58 SS.reconfigure :inhaling 0.84ml/s @19:48:25.63 SS.seek 25ml @19:48:29.17 Stroke completed -- waiting for pressure to equalize ... @19:48:29.18 SS.reconfigure :exhale @19:48:29.22 SS.hold @19:48:30.51 Intake.select :close @19:48:30.66 SSV.dial :exhaust @19:48:31.37 Exhaust.select :open @19:48:31.48 SS.seek 0ml @19:48:40.81 Exhaust.select :close @19:48:40.91 SSV.dial CBV @19:48:40.94
CBV.dial SSV @19:48:41.71 Allowing 130s for 25.0ml of remaining 450.0ml @19:48:41.72 Intake.select :open @19:48:43.87 Intake @14.3psia, vacuum from 8.8 to 11.8psi @19:48:43.88 SS.reconfigure :inhaling 0.84ml/s @19:48:43.93 SS.seek 25ml @19:49:14.98 Stroke completed -- waiting for pressure to equalize ... @19:49:14.99 SS.reconfigure :exhale @19:49:15.03 SS.hold @19:49:16.67 Intake.select :close @19:49:16.81 SSV.dial :exhaust @19:49:17.55 Exhaust.select :open @19:49:17.65 SS.seek 0ml @19:49:27.09 Exhaust.select :close @19:49:27.19 SSV.dial CBV @19:49:27.22
CBV.dial SSV @19:49:27.97 Allowing 130s for 25.0ml of remaining 425.0ml @19:49:27.98 Intake.select :open @19:49:30.13 Intake @14.4psia, vacuum from 8.9 to 11.9psi @19:49:30.15 SS.reconfigure :inhaling 0.84ml/s @19:49:30.19 SS.seek 25ml @19:50:01.46 Stroke completed -- waiting for pressure to equalize ... @19:50:01.47 SS.reconfigure :exhale @19:50:01.51 SS.hold @19:50:03.47 Intake.select :close @19:50:03.61 SSV.dial :exhaust @19:50:04.34 Exhaust.select :open @19:50:04.45 SS.seek 0ml @19:50:13.71 Exhaust.select :close @19:50:13.82 SSV.dial CBV @19:50:13.84
CBV.dial SSV @19:50:14.61 Allowing 130s for 25.0ml of remaining 400.0ml @19:50:14.62 Intake.select :open @19:50:16.77 Intake @14.5psia, vacuum from 9.0 to 12.0psi @19:50:16.79 SS.reconfigure :inhaling 0.84ml/s @19:50:16.83 SS.seek 25ml @19:50:47.92 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @19:50:47.96 SS.hold @19:50:50.36 Intake.select :close @19:50:50.51 SSV.dial :exhaust @19:50:51.25 Exhaust.select :open @19:50:51.35 SS.seek 0ml @19:51:00.77 Exhaust.select :close @19:51:00.88 SSV.dial CBV @19:51:00.90
CBV.dial SSV @19:51:01.65 Allowing 130s for 25.0ml of remaining 375.0ml @19:51:01.66 Intake.select :open @19:51:03.81 Intake @14.5psia, vacuum from 9.0 to 12.0psi @19:51:03.83 SS.reconfigure :inhaling 0.84ml/s @19:51:03.88 SS.seek 25ml @19:51:35.05 Stroke completed -- waiting for pressure to equalize ... @19:51:35.06 SS.reconfigure :exhale @19:51:35.10 SS.hold @19:51:37.51 Intake.select :close @19:51:37.65 SSV.dial :exhaust @19:51:38.37 Exhaust.select :open @19:51:38.48 SS.seek 0ml @19:51:47.88 Exhaust.select :close @19:51:47.99 SSV.dial CBV @19:51:48.01
CBV.dial SSV @19:51:48.78 Allowing 130s for 25.0ml of remaining 350.0ml @19:51:48.79 Intake.select :open @19:51:50.94 Intake @14.4psia, vacuum from 8.9 to 11.9psi @19:51:50.95 SS.reconfigure :inhaling 0.84ml/s @19:51:51.00 SS.seek 25ml @19:52:15.58 Vacuum too high -- waiting up to 40 seconds @19:52:15.59 SS.reconfigure :exhale @19:52:15.63 SS.hold @19:52:16.58 Intake @12.9psia, vacuum from 7.4 to 10.4psi @19:52:16.60 SS.reconfigure :inhaling 0.76ml/s @19:52:16.64 SS.seek 25ml @19:52:24.34 Stroke completed -- waiting for pressure to equalize ... @19:52:24.35 SS.reconfigure :exhale @19:52:24.39 SS.hold @19:52:26.06 Intake.select :close @19:52:26.21 SSV.dial :exhaust @19:52:26.95 Exhaust.select :open @19:52:27.05 SS.seek 0ml @19:52:36.01 Exhaust.select :close @19:52:36.11 SSV.dial CBV @19:52:36.14
CBV.dial SSV @19:52:36.89 Allowing 130s for 25.0ml of remaining 325.0ml @19:52:36.90 Intake.select :open @19:52:39.05 Intake @14.5psia, vacuum from 9.0 to 12.0psi @19:52:39.07 SS.reconfigure :inhaling 0.76ml/s @19:52:39.11 SS.seek 25ml @19:53:14.45 Stroke completed -- waiting for pressure to equalize ... @19:53:14.46 SS.reconfigure :exhale @19:53:14.50 SS.hold @19:53:17.40 Intake.select :close @19:53:17.54 SSV.dial :exhaust @19:53:18.27 Exhaust.select :open @19:53:18.38 SS.seek 0ml @19:53:27.41 Exhaust.select :close @19:53:27.51 SSV.dial CBV @19:53:27.54
CBV.dial SSV @19:53:28.27 Allowing 130s for 25.0ml of remaining 300.0ml @19:53:28.28 Intake.select :open @19:53:30.44 Intake @14.5psia, vacuum from 9.0 to 12.0psi @19:53:30.45 SS.reconfigure :inhaling 0.76ml/s @19:53:30.50 SS.seek 25ml @19:54:05.86 Stroke completed -- waiting for pressure to equalize ... @19:54:05.87 SS.reconfigure :exhale @19:54:05.91 SS.hold @19:54:08.90 Intake.select :close @19:54:09.04 SSV.dial :exhaust @19:54:09.77 Exhaust.select :open @19:54:09.88 SS.seek 0ml @19:54:18.88 Exhaust.select :close @19:54:18.98 SSV.dial CBV @19:54:19.01
CBV.dial SSV @19:54:19.74 Allowing 130s for 25.0ml of remaining 275.0ml @19:54:19.75 Intake.select :open @19:54:21.90 Intake @14.6psia, vacuum from 9.1 to 12.1psi @19:54:21.92 SS.reconfigure :inhaling 0.76ml/s @19:54:21.96 SS.seek 25ml @19:54:57.33 Stroke completed -- waiting for pressure to equalize ... @19:54:57.34 SS.reconfigure :exhale @19:54:57.38 SS.hold @19:55:00.89 Intake.select :close @19:55:01.03 SSV.dial :exhaust @19:55:01.77 Exhaust.select :open @19:55:01.88 SS.seek 0ml @19:55:10.88 Exhaust.select :close @19:55:10.98 SSV.dial CBV @19:55:11.01
CBV.dial SSV @19:55:11.77 Allowing 130s for 25.0ml of remaining 250.0ml @19:55:11.78 Intake.select :open @19:55:13.93 Intake @14.7psia, vacuum from 9.2 to 12.2psi @19:55:13.95 SS.reconfigure :inhaling 0.76ml/s @19:55:13.99 SS.seek 25ml @19:55:48.76 Vacuum too high -- waiting up to 40 seconds @19:55:48.77 SS.reconfigure :exhale @19:55:48.81 SS.hold @19:55:50.92 Intake @14.1psia, vacuum from 8.6 to 11.6psi @19:55:50.94 SS.reconfigure :inhaling 0.69ml/s @19:55:50.98 SS.seek 25ml @19:55:52.00 Stroke completed -- waiting for pressure to equalize ... @19:55:52.01 SS.reconfigure :exhale @19:55:52.05 SS.hold @19:55:53.33 Intake.select :close @19:55:53.47 SSV.dial :exhaust @19:55:54.20 Exhaust.select :open @19:55:54.31 SS.seek 0ml @19:56:03.59 Exhaust.select :close @19:56:03.70 SSV.dial CBV @19:56:03.72
CBV.dial SSV @19:56:04.47 Allowing 130s for 25.0ml of remaining 225.0ml @19:56:04.48 Intake.select :open @19:56:06.63 Intake @14.7psia, vacuum from 9.2 to 12.2psi @19:56:07.09 SS.reconfigure :inhaling 0.69ml/s @19:56:07.14 SS.seek 25ml @19:56:44.95 Stroke completed -- waiting for pressure to equalize ... @19:56:44.96 SS.reconfigure :exhale @19:56:45.00 SS.hold @19:56:47.99 Intake.select :close @19:56:48.13 SSV.dial :exhaust @19:56:48.86 Exhaust.select :open @19:56:48.97 SS.seek 0ml @19:56:57.92 Exhaust.select :close @19:56:58.02 SSV.dial CBV @19:56:58.05
CBV.dial SSV @19:56:58.78 Allowing 130s for 25.0ml of remaining 200.0ml @19:56:58.79 Intake.select :open @19:57:00.95 Intake @14.6psia, vacuum from 9.1 to 12.1psi @19:57:00.96 SS.reconfigure :inhaling 0.69ml/s @19:57:01.00 SS.seek 25ml @19:57:39.93 Stroke completed -- waiting for pressure to equalize ... @19:57:39.94 SS.reconfigure :exhale @19:57:39.98 SS.hold @19:57:43.43 Intake.select :close @19:57:43.57 SSV.dial :exhaust @19:57:44.31 Exhaust.select :open @19:57:44.42 SS.seek 0ml @19:57:53.81 Exhaust.select :close @19:57:53.91 SSV.dial CBV @19:57:53.96
CBV.dial SSV @19:57:54.69 Allowing 130s for 25.0ml of remaining 175.0ml @19:57:54.70 Intake.select :open @19:57:56.85 Intake @14.7psia, vacuum from 9.2 to 12.2psi @19:57:56.87 SS.reconfigure :inhaling 0.69ml/s @19:57:56.91 SS.seek 25ml @19:58:34.85 Stroke completed -- waiting for pressure to equalize ... @19:58:34.86 SS.reconfigure :exhale @19:58:34.90 SS.hold @19:58:38.35 Intake.select :close @19:58:38.49 SSV.dial :exhaust @19:58:39.23 Exhaust.select :open @19:58:39.34 SS.seek 0ml @19:58:48.78 Exhaust.select :close @19:58:48.88 SSV.dial CBV @19:58:48.91
CBV.dial SSV @19:58:49.65 Allowing 130s for 25.0ml of remaining 150.0ml @19:58:49.66 Intake.select :open @19:58:51.82 Intake @14.7psia, vacuum from 9.2 to 12.2psi @19:58:51.83 SS.reconfigure :inhaling 0.69ml/s @19:58:51.87 SS.seek 25ml @19:59:23.16 Vacuum too high -- waiting up to 40 seconds @19:59:23.17 SS.reconfigure :exhale @19:59:23.21 SS.hold @19:59:24.40 Intake @13.4psia, vacuum from 7.9 to 10.9psi @19:59:24.42 SS.reconfigure :inhaling 0.61ml/s @19:59:24.46 SS.seek 25ml @19:59:32.26 Stroke completed -- waiting for pressure to equalize ... @19:59:32.27 SS.reconfigure :exhale @19:59:32.31 SS.hold @19:59:34.81 Intake.select :close @19:59:34.95 SSV.dial :exhaust @19:59:35.68 Exhaust.select :open @19:59:35.79 SS.seek 0ml @19:59:45.09 Exhaust.select :close @19:59:45.19 SSV.dial CBV @19:59:45.24
CBV.dial SSV @19:59:45.96 Allowing 130s for 25.0ml of remaining 125.0ml @19:59:45.97 Intake.select :open @19:59:48.13 Intake @14.7psia, vacuum from 9.2 to 12.2psi @19:59:48.14 SS.reconfigure :inhaling 0.61ml/s @19:59:48.18 SS.seek 25ml @20:00:30.43 Stroke completed -- waiting for pressure to equalize ... @20:00:30.44 SS.reconfigure :exhale @20:00:30.48 SS.hold @20:00:33.94 Intake.select :close @20:00:34.08 SSV.dial :exhaust @20:00:34.82 Exhaust.select :open @20:00:35.38 SS.seek 0ml @20:00:44.79 Exhaust.select :close @20:00:44.89 SSV.dial CBV @20:00:44.94
CBV.dial SSV @20:00:45.65 Allowing 130s for 25.0ml of remaining 99.9ml @20:00:45.66 Intake.select :open @20:00:47.81 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:00:47.83 SS.reconfigure :inhaling 0.61ml/s @20:00:47.87 SS.seek 25ml @20:01:30.19 Stroke completed -- waiting for pressure to equalize ... @20:01:30.20 SS.reconfigure :exhale @20:01:30.24 SS.hold @20:01:34.42 Intake.select :close @20:01:34.56 SSV.dial :exhaust @20:01:35.29 Exhaust.select :open @20:01:35.40 SS.seek 0ml @20:01:44.36 Exhaust.select :close @20:01:44.47 SSV.dial CBV @20:01:44.49
CBV.dial SSV @20:01:45.26 Allowing 130s for 25.0ml of remaining 74.9ml @20:01:45.27 Intake.select :open @20:01:47.42 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:01:47.44 SS.reconfigure :inhaling 0.61ml/s @20:01:47.48 SS.seek 25ml @20:02:30.10 Stroke completed -- waiting for pressure to equalize ... @20:02:30.11 SS.reconfigure :exhale @20:02:30.15 SS.hold @20:02:34.31 Intake.select :close @20:02:34.45 SSV.dial :exhaust @20:02:35.20 Exhaust.select :open @20:02:35.31 SS.seek 0ml @20:02:44.86 Exhaust.select :close @20:02:44.97 SSV.dial CBV @20:02:44.99
CBV.dial SSV @20:02:45.74 Allowing 130s for 25.0ml of remaining 49.9ml @20:02:45.75 Intake.select :open @20:02:47.91 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:02:47.92 SS.reconfigure :inhaling 0.61ml/s @20:02:47.97 SS.seek 25ml @20:03:30.02 Vacuum too high -- waiting up to 40 seconds @20:03:30.03 SS.reconfigure :exhale @20:03:30.07 SS.hold @20:03:32.56 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:03:32.58 SS.reconfigure :inhaling 0.56ml/s @20:03:32.62 SS.seek 25ml @20:03:33.37 Stroke completed -- waiting for pressure to equalize ... @20:03:33.38 SS.reconfigure :exhale @20:03:33.43 SS.hold @20:03:35.39 Intake.select :close @20:03:35.53 SSV.dial :exhaust @20:03:36.25 Exhaust.select :open @20:03:36.36 SS.seek 0ml @20:03:45.28 Exhaust.select :close @20:03:45.38 SSV.dial CBV @20:03:45.41
CBV.dial SSV @20:03:46.14 Allowing 130s for 24.9ml of remaining 24.9ml @20:03:46.15 Intake.select :open @20:03:48.31 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:03:48.32 SS.reconfigure :inhaling 0.56ml/s @20:03:48.37 SS.seek 24.94ml @20:04:36.03 Stroke completed -- waiting for pressure to equalize ... @20:04:36.04 SS.reconfigure :exhale @20:04:36.08 SS.hold @20:04:40.14 Intake.select :close @20:04:40.28 SSV.dial :exhaust @20:04:41.04 Exhaust.select :open @20:04:41.15 SS.seek 0ml @20:04:50.20 Exhaust.select :close @20:04:50.31 Sampled 1000.0ml @20:04:50.32 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @20:04:50.33 Intake.select :close @20:04:50.39 CSV.dial :waste @20:04:50.43 CS.seek 0ml @20:04:50.53 CSV.dial :air @20:04:50.59 SSV.dial CBV,avoiding: :exhaust @20:04:50.98
CBV.dial SSV @20:04:51.29 CS.seek 10ml @20:04:52.93
CTV.dial :air @20:04:53.67 delay 15 seconds @20:05:08.69 SSV.dial :spit,avoiding: :exhaust @20:05:10.02 CTV.dial CSV,avoiding: :intake @20:05:10.06
SS.seek 0ml @20:05:10.16 SSV.dial CBV,avoiding: :exhaust @20:05:14.66 CBV.dial between SSV and waste2 @20:05:15.05 CTV.dial CSV,avoiding: :air @20:05:15.07
CSV.dial CTV @20:05:15.52 CS.reconfigure :pressurize @20:05:15.57 CS.seek 0ml @20:05:34.29 BubblePoint: Intake @40.6psia, Outlet @16.4psia @20:05:34.30 CBV.dial SSV @20:05:34.63 SS.reconfigure :default @20:05:34.67 SS.seek 10ml @20:05:40.41 delay 15 seconds @20:05:55.52 Evacuated: Intake @16.9psia, Outlet @10.4psia @20:05:55.53 SSV.dial :spit,avoiding: :exhaust @20:05:56.85 SS.reconfigure :exhale @20:05:56.89 SS.seek 0ml @20:06:02.26 SS.reconfigure :fast @20:06:02.30 SS.seek 5ml @20:06:05.03 SSV.dial CBV,avoiding: :exhaust @20:06:05.06
CBV.dial SSV @20:06:06.38 CTV.dial :air,avoiding: :intake @20:06:07.66 CSV.dial CTV @20:06:07.69
SS.seek 10ml @20:06:08.69 CSV.dial :air @20:06:09.14 CS.reconfigure :fast @20:06:09.18 CS.seek 0ml @20:06:10.56
SSV.dial :spit,avoiding: :exhaust @20:06:11.89 SS.reconfigure :exhale @20:06:11.94 SS.seek 0ml @20:06:16.26 CS.stop @20:06:17.43
SS.reconfigure :fast @20:06:17.47 SS.seek 5ml @20:06:20.10 SSV.dial CBV,avoiding: :exhaust @20:06:20.12
CBV.dial SSV @20:06:22.46 SSV.dial :spit,avoiding: :exhaust @20:06:23.78 SS.reconfigure :default @20:06:23.83 SS.seek 0ml @20:06:27.12 SS.stop @20:06:27.16 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @20:06:27.17 Intake.select :close @20:06:27.23 CSV.dial :waste @20:06:27.34 SSV.dial CBV,avoiding: :exhaust @20:06:27.37
CBV.dial SSV @20:06:28.02 CS.seek 0ml @20:06:28.08 CSV.dial :air @20:06:28.70
CTV.dial :air @20:06:28.72 delay 15 seconds @20:06:28.82 CS.seek 10ml @20:06:43.73
SSV.dial :spit,avoiding: :exhaust @20:06:45.06 CTV.dial CSV,avoiding: :intake @20:06:45.10
SS.reconfigure :exhale @20:06:45.19 SS.seek 0ml @20:06:45.24 SSV.dial CBV,avoiding: :exhaust @20:06:51.94 CBV.dial between SSV and waste2 @20:06:52.32 CTV.dial CSV,avoiding: :air @20:06:52.35
CSV.dial CTV @20:06:52.78 CS.reconfigure :pressurize @20:06:52.83 CS.seek 0ml @20:07:14.11 BubblePoint: Intake @39.5psia, Outlet @15.4psia @20:07:14.12 CBV.dial SSV @20:07:14.44 SS.reconfigure :default @20:07:14.48 SS.seek 10ml @20:07:20.01 delay 15 seconds @20:07:35.13 Evacuated: Intake @19.0psia, Outlet @12.4psia @20:07:35.15 SSV.dial :spit,avoiding: :exhaust @20:07:36.47 SS.reconfigure :exhale @20:07:36.52 SS.seek 0ml @20:07:42.01 SS.reconfigure :fast @20:07:42.05 SS.seek 5ml @20:07:44.86 SSV.dial CBV,avoiding: :exhaust @20:07:44.88
CBV.dial SSV @20:07:46.20 CTV.dial :air,avoiding: :intake @20:07:47.50 CSV.dial CTV @20:07:47.53
SS.seek 10ml @20:07:48.54 CSV.dial :air @20:07:49.00 CS.reconfigure :fast @20:07:49.05 CS.seek 0ml @20:07:50.04
SSV.dial :spit,avoiding: :exhaust @20:07:51.38 SS.reconfigure :exhale @20:07:51.42 SS.seek 0ml @20:07:53.76 CS.stop @20:07:56.77
SS.reconfigure :fast @20:07:56.81 SS.seek 5ml @20:07:59.59 SSV.dial CBV,avoiding: :exhaust @20:07:59.62
CBV.dial SSV @20:08:01.94 SSV.dial :spit,avoiding: :exhaust @20:08:03.25 SS.reconfigure :default @20:08:03.29 SS.seek 0ml @20:08:06.62 SS.stop @20:08:06.65 Lysing: Adding 2.0ml AqMeOH @20:08:06.67 CBV.dial between CSV and waste1,avoiding: :DBV @20:08:08.14 CTV.dial CSV @20:08:09.43 CSV.dial CSR @20:08:10.20 Pulling 0.999ml of CSR.AqMeOH @20:08:10.21 CSR.select :AqMeOH @20:08:10.32 CS.reconfigure :default @20:08:10.36 CS.seek 1ml @20:08:13.67 delay 5 seconds @20:08:18.68 CSR.select :air @20:08:18.84 CS.seek 6ml @20:08:32.23 delay 2 seconds @20:08:34.24 CSV.dial :waste2 @20:08:34.67 CS.reconfigure :fast @20:08:34.71 CS.seek 0ml @20:08:48.66 CSV.dial CSR @20:08:49.17 Pulling 2ml of CSR.AqMeOH @20:08:49.18 CSR.select :AqMeOH @20:08:49.29 CS.reconfigure :default @20:08:49.33 CS.seek 2ml @20:08:55.13 delay 5 seconds @20:09:00.15 CSR.select :air @20:09:00.30 CS.seek 5ml @20:09:08.66 delay 2 seconds @20:09:10.68 CSV.dial CTV @20:09:11.12 CS.seek 1ml @20:09:22.08 Before lysing Collection Puck Heater at 9.6C/38.7psia @20:09:22.10 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @20:09:22.11 CH.seek 70C,8:00 @20:11:33.11 CH.hold 10:00,70C @20:11:33.30 Begun lysing Collection Puck Heater at 68C/42.5psia @20:21:33.30 Ended lysing Collection Puck Heater at 70C/44.0psia @20:21:33.31 CS.seek 0ml @20:21:36.59 CTV.dial between spare and air,avoiding: :intake @20:21:37.58 CSV.dial :air @20:21:38.64 CS.seek 5ml @20:21:52.02 CSV.dial CTV @20:21:52.46 delay 2 seconds @20:21:54.49 CSV.dial :air @20:21:54.94 delay 2 seconds @20:21:56.96 CSV.dial CTV @20:21:57.42 delay 2 seconds @20:21:59.43 CSV.dial :air @20:21:59.88 delay 2 seconds @20:22:01.90 CS.seek 0ml @20:22:15.38 Puck @71.7psia after final lysis pressurization @20:22:15.39 Recovering Lysate into Collection syringe @20:22:15.40 CSV.dial CBV @20:22:16.53 CBV.dial CSV @20:22:16.93 CS.seek 5.001ml @20:22:30.30 delay 5 seconds @20:22:35.32 CTV.dial :air @20:22:35.83 CS.seek 7ml @20:22:41.64 delay 5 seconds @20:22:46.66 CSV.dial :air2 @20:22:47.24 CS.seek 10ml @20:22:55.64 CSV.dial between air and CTV,avoiding: CBV @20:22:56.80 Lysing Complete @20:22:56.87 Storing sh1 puck in Collection Clamp to tube 2 @20:22:57.03 SC.reconfigure :tight @20:22:57.08 SC.seek 2 @20:23:00.65 SC.configure :limp @20:23:00.84 Elbow.reconfigure :tight @20:23:00.88 Elbow.seek 2 @20:23:02.56 Forearm.reconfigure :tight @20:23:02.60 Forearm.seek 2 + 700 counts @20:23:03.60 SE.reconfigure :pick @20:23:03.65 SE.seek :beamBreak @20:23:05.55 SE.configure :default @20:23:05.71 Elbow.reconfigure :limp @20:23:05.75 Forearm.reconfigure :loose @20:23:05.79 Forearm.seek 2 @20:23:06.20 Elbow.hold @20:23:06.26 Elbow.reconfigure :tight @20:23:06.34 SE.seek 18.31 Pucks below Top Plate @20:23:06.87 Forearm.force -20 @20:23:06.91 Forearm.seek 2 + 296 counts @20:23:07.20 Forearm.reconfigure :tight @20:23:07.24 Forearm.seek 2 + 700 counts @20:23:07.56 SE.seek 19.31 Pucks below Top Plate @20:23:08.12 Forearm.reconfigure :loose @20:23:08.16 Forearm.seek :retract @20:23:09.07 Elbow.seek CC @20:23:10.61 Elbow.reconfigure :loose @20:23:10.70 Forearm.seek CC @20:23:11.73 Hand.close @20:23:12.46 CC.seek :open @20:23:16.92 Forearm.seek :retract @20:23:17.92 Elbow.reconfigure :tight @20:23:17.96 Elbow.seek 2 @20:23:19.69 Elbow.reconfigure :limp @20:23:19.74 Forearm.seek 2 @20:23:20.81 Elbow.hold @20:23:20.87 Elbow.reconfigure :tight @20:23:20.91 Hand.open @20:23:21.71 Forearm.force -20 @20:23:21.74 Forearm.seek 2 + 320 counts @20:23:22.02 Forearm.reconfigure :tight @20:23:22.06 Forearm.seek 2 + 700 counts @20:23:22.43 SE.seek :down @20:23:23.55
Forearm.reconfigure :loose @20:23:23.59 Forearm.seek :retract @20:23:23.85 SE.stop @20:23:24.51
Tranferring DA @20:23:24.53 Beginning samplePassC @20:23:24.54 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:23:24.72 Elbow.seek FlushPuck::Garage @20:23:26.52 Elbow.reconfigure :loose @20:23:26.61 Forearm.seek :garage @20:23:27.71 Hand.close @20:23:29.03 Forearm.seek :retract @20:23:30.07 Elbow.reconfigure :tight @20:23:30.12 Elbow.seek CC @20:23:33.04 Elbow.reconfigure :loose @20:23:33.13 Forearm.reconfigure :tight @20:23:33.17 Forearm.seek CC @20:23:34.45 CC.seek :closed @20:23:38.69 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.86 @20:23:38.77 Hand.open @20:23:39.52 Forearm.reconfigure :loose @20:23:39.56 Forearm.seek :retract @20:23:40.54 PSV.dial PSR @20:23:41.88 Pulling 1.5ml of PSR.flush PSR.select :flush @20:23:42.00 PS.seek 1.5ml @20:23:58.01 delay 5 seconds @20:24:03.03 PSV.dial :waste1 @20:24:03.59 PS.seek 0ml @20:24:19.63 PSV.dial PSR @20:24:20.05 PSR.select :air @20:24:20.17 PS.seek 2.5ml @20:24:46.39 PRV.dial CBV,avoiding: :ab2 @20:24:46.41
CBV.dial PRV @20:24:48.07 PSV.dial :mixloop @20:24:49.07 CTV.dial :air,avoiding: :intake @20:24:49.09 PTV.dial :PRVmixing @20:24:50.36 PS.seek 0ml @20:25:16.47 PSV.dial :air @20:25:16.92 PS.seek 2.5ml @20:25:43.07 PSV.dial :mixloop @20:25:43.51 PS.seek 0ml @20:26:09.69 PSV.dial :air @20:26:10.14 PS.seek 2.5ml @20:26:36.38 PSV.dial :mixloop @20:26:36.82 PS.seek 0ml @20:27:02.94 Garaging FlushPuck in Collection Clamp @20:27:03.01 Elbow.reconfigure :tight @20:27:03.05 Elbow.seek CC @20:27:03.17 Elbow.reconfigure :loose @20:27:03.26 Forearm.seek CC @20:27:04.20 Hand.close @20:27:04.96 CC.seek :open @20:27:09.28 Forearm.seek :retract @20:27:10.32 Elbow.reconfigure :tight @20:27:10.36 Elbow.seek :garage @20:27:13.25 Elbow.reconfigure :loose @20:27:13.33 Forearm.reconfigure :tight @20:27:13.38 Forearm.seek :garage @20:27:14.48 Hand.open @20:27:15.26 Forearm.reconfigure :loose @20:27:15.31 Forearm.seek :retract @20:27:16.39 PSV.dial :mixloop @20:27:16.40 PTV.dial :PRVmixing @20:27:16.42 PRV.dial CBV @20:27:16.44
CBV.dial PRV @20:27:16.46 CTV.dial :air,avoiding: :intake @20:27:16.47 Loading lyfil puck from tube 3 into Collection Clamp @20:27:16.68 SC.reconfigure :tight @20:27:16.73 SC.seek 3 @20:27:20.29 SC.configure :limp @20:27:20.48 Elbow.reconfigure :tight @20:27:20.53 Elbow.seek 3 @20:27:22.52 Forearm.reconfigure :tight @20:27:22.56 Forearm.seek 3 + 700 counts @20:27:23.67 SE.reconfigure :pick @20:27:23.72 SE.seek :beamBreak @20:27:26.05 SE.configure :default @20:27:26.65 Elbow.seek 3 @20:27:26.82 Forearm.seek 3 + 300 counts @20:27:27.02 Forearm.reconfigure :loose @20:27:27.06 Hand.close @20:27:27.72 SE.seek :down @20:27:27.91
Forearm.seek :retract @20:27:28.94 Elbow.seek CC @20:27:29.70 SE.stop @20:27:30.50
Elbow.reconfigure :loose @20:27:30.59 Forearm.reconfigure :tight @20:27:30.63 Forearm.seek CC @20:27:31.85 CC.seek :closed @20:27:36.09 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.65 @20:27:36.10 Hand.open @20:27:36.89 Forearm.reconfigure :loose @20:27:36.94 Forearm.seek :retract @20:27:37.92 CTV.dial CSV,avoiding: :intake @20:27:39.21 CSV.dial CTV @20:27:39.66 PS.seek 2.5ml @20:28:05.87 CS.seek 5.5ml @20:28:18.07 delay 8 seconds @20:28:26.09 PSV.dial :air @20:28:26.53 delay 2 seconds @20:28:28.55 PSV.dial :waste2 @20:28:29.40 PS.seek 0ml @20:28:55.56 CBV.dial between PRV and waste1 @20:28:55.89 CSV.dial :air @20:28:56.33 CS.seek 10ml @20:29:08.54 CSV.dial CTV @20:29:09.00 CS.seek 0ml @20:29:35.07 CBV.dial :waste1 @20:29:35.37 delay 5 seconds @20:29:40.39 CTV.dial :air,avoiding: :intake @20:29:41.72 Storing lyfil puck in Collection Clamp to tube 2 @20:29:41.88 SC.reconfigure :tight @20:29:41.92 SC.seek 2 @20:29:45.48 SC.configure :limp @20:29:45.67 Elbow.reconfigure :tight @20:29:45.72 Elbow.seek 2 @20:29:47.45 Forearm.reconfigure :tight @20:29:47.49 Forearm.seek 2 + 700 counts @20:29:48.43 SE.reconfigure :pick @20:29:48.48 SE.seek :beamBreak @20:29:50.31 SE.configure :default @20:29:50.45 Elbow.reconfigure :limp @20:29:50.50 Forearm.reconfigure :loose @20:29:50.54 Forearm.seek 2 @20:29:50.94 Elbow.hold @20:29:51.00 Elbow.reconfigure :tight @20:29:51.09 SE.seek 19.32 Pucks below Top Plate @20:29:51.60 Forearm.force -20 @20:29:51.63 Forearm.seek 2 + 317 counts @20:29:51.92 Forearm.reconfigure :tight @20:29:51.96 Forearm.seek 2 + 700 counts @20:29:52.17 SE.seek 20.32 Pucks below Top Plate @20:29:52.77 Forearm.reconfigure :loose @20:29:52.81 Forearm.seek :retract @20:29:53.78 Elbow.seek CC @20:29:55.43 Elbow.reconfigure :loose @20:29:55.52 Forearm.seek CC @20:29:56.51 Hand.close @20:29:57.60 CC.seek :open @20:30:02.10 Forearm.seek :retract @20:30:03.14 Elbow.reconfigure :tight @20:30:03.18 Elbow.seek 2 @20:30:05.03 Elbow.reconfigure :limp @20:30:05.07 Forearm.seek 2 @20:30:06.21 Elbow.hold @20:30:06.28 Elbow.reconfigure :tight @20:30:06.33 Hand.open @20:30:07.20 Forearm.force -20 @20:30:07.23 Forearm.seek 2 + 314 counts @20:30:07.58 Forearm.reconfigure :tight @20:30:07.63 Forearm.seek 2 + 700 counts @20:30:07.98 SE.seek :down @20:30:09.11
Forearm.reconfigure :loose @20:30:09.15 Forearm.seek :retract @20:30:09.32 SE.stop @20:30:10.11
PSV.dial :air @20:30:10.80 PS.seek 2.5ml @20:30:37.00 delay 1 second @20:30:38.01 PSV.dial :mixloop @20:30:38.45 delay 1 second @20:30:39.47 PSV.dial :air @20:30:39.91 delay 1 second @20:30:40.92 PSV.dial :mixloop @20:30:41.36 delay 1 second @20:30:42.38 PSV.dial :air @20:30:42.83 delay 1 second @20:30:43.85 PS.seek 0ml @20:31:10.03 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @20:31:10.20 Elbow.seek FlushPuck::Garage @20:31:12.02 Elbow.reconfigure :loose @20:31:12.10 Forearm.seek :garage @20:31:13.20 Hand.close @20:31:13.94 Forearm.seek :retract @20:31:14.98 Elbow.reconfigure :tight @20:31:15.02 Elbow.seek PC @20:31:16.49 Elbow.reconfigure :loose @20:31:16.57 Forearm.reconfigure :tight @20:31:16.62 Forearm.seek PC @20:31:17.97 PC.seek :closed @20:31:21.84 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.38 @20:31:21.91 Hand.open @20:31:22.76 Forearm.reconfigure :loose @20:31:22.80 Forearm.seek :retract @20:31:23.87 PRV.dial :air @20:31:24.31 PSV.dial :mixloop @20:31:24.82 PS.seek 0.3ml @20:31:28.65 PSV.dial PSR @20:31:29.69 Pulling 0.4ml of PSR.air @20:31:29.70 PSR.select :air @20:31:29.72 PS.seek 0.7ml @20:31:34.59 delay 3 seconds @20:31:37.60 PSR.select :air @20:31:37.63 PS.seek 2.5ml @20:31:56.72 delay 3 seconds @20:31:59.73 PSV.dial :mixloop @20:32:00.73 PTV.dial :mixing @20:32:01.43 PBV.dial :waste2 @20:32:02.35 PS.seek 0ml @20:32:28.53 Garaging FlushPuck in Processing Clamp @20:32:28.60 Elbow.reconfigure :tight @20:32:28.64 Elbow.seek PC @20:32:28.87 Elbow.reconfigure :loose @20:32:28.96 Forearm.seek PC @20:32:30.05 Hand.close @20:32:30.70 PC.seek :open @20:32:35.11 Forearm.seek :retract @20:32:36.15 Elbow.reconfigure :tight @20:32:36.19 Elbow.seek :garage @20:32:37.56 Elbow.reconfigure :loose @20:32:37.65 Forearm.reconfigure :tight @20:32:37.70 Forearm.seek :garage @20:32:38.87 Hand.open @20:32:39.64 Forearm.reconfigure :loose @20:32:39.68 Forearm.seek :retract @20:32:40.70 Loading da puck from tube 3 into Processing Clamp @20:32:40.92 SC.reconfigure :tight @20:32:40.96 SC.seek 3 @20:32:44.54 SC.configure :limp @20:32:44.74 Elbow.reconfigure :tight @20:32:44.78 Elbow.seek 3 @20:32:46.70 Forearm.reconfigure :tight @20:32:46.74 Forearm.seek 3 + 700 counts @20:32:47.80 SE.reconfigure :pick @20:32:47.85 SE.seek :beamBreak @20:32:50.23 SE.configure :default @20:32:50.38 Elbow.seek 3 @20:32:50.55 Forearm.seek 3 + 300 counts @20:32:50.74 Forearm.reconfigure :loose @20:32:50.78 Hand.close @20:32:51.46 SE.seek :down @20:32:51.65
Forearm.seek :retract @20:32:52.60 Elbow.seek PC @20:32:53.51 SE.stop @20:32:55.21
Elbow.reconfigure :loose @20:32:55.30 Forearm.reconfigure :tight @20:32:55.34 Forearm.seek PC @20:32:56.69 PC.seek :closed @20:33:00.55 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.35 @20:33:00.56 Hand.open @20:33:01.38 Forearm.reconfigure :loose @20:33:01.42 Forearm.seek :retract @20:33:02.42 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @20:33:02.44 PH.seek 28C,5:00 @20:33:53.85 PH.hold 2:00:00,28C @20:33:53.90 delay 1:30 @20:35:23.93 PSR.select :air @20:35:23.95 PSV.dial PSR @20:35:25.01 Pulling 0.5ml of PSR.wash @20:35:25.02 PSR.select :wash @20:35:25.14 PS.seek 0.5ml @20:35:31.12 delay 5 seconds @20:35:36.14 PSR.select :air @20:35:36.25 PS.seek 2ml @20:35:52.21 PTV.dial :puck @20:35:52.91 PSV.dial PTV @20:35:53.65 PBV.dial :waste1 @20:35:54.99 PS.seek 1.5ml @20:36:00.93 delay 3 seconds @20:36:03.94 PBV.dial between PSV and waste1 @20:36:04.31 PS.seek 0ml @20:36:20.27 delay 5:00 @20:41:20.30 recoverPSRreagent08 PTV.dial :puck @20:41:20.78 PBV.dial :waste1 @20:41:21.14 delay 30 seconds @20:41:51.16 PBV.dial between PSV and waste1 @20:41:51.53 PSR.select :air @20:41:51.55 PSV.dial PSR @20:41:52.28 PS.reconfigure :fast @20:41:52.33 PS.seek 2.5ml @20:42:15.26 PSV.dial PTV @20:42:16.00 delay 5 seconds @20:42:21.02 PSV.dial PSR @20:42:21.75 delay 5 seconds @20:42:26.77 PSV.dial PTV @20:42:27.61 delay 5 seconds @20:42:32.63 PSV.dial :air @20:42:33.38 PS.seek 0ml @20:42:56.14 PTV.dial :PRVmixing,via: PRV @20:42:57.40 PSV.dial :mixloop @20:42:57.84 PRV.dial :ab1 @20:42:58.60 PS.reconfigure :slow @20:42:58.64 PS.seek 0.15ml @20:43:02.23 delay 2 seconds @20:43:04.25 CBV.dial PRV @20:43:04.27
PRV.dial CBV @20:43:04.84 PS.seek 0.34ml @20:43:09.26 delay 2 seconds @20:43:11.28 CBV.dial between PRV and PSV @20:43:11.58 PRV.dial :air @20:43:12.03 PS.seek 0.9ml @20:43:23.95 PRV.dial :waste2 @20:43:24.67 PS.seek 0.5ml @20:43:33.26 addPMLReagent08 @20:43:33.27 PSV.dial :air @20:43:33.72 PS.reconfigure :default @20:43:33.76 PS.seek 2ml @20:43:49.76 PSV.dial :PML @20:43:50.20 PTV.dial :mixing @20:43:50.92 PBV.dial :waste1 @20:43:51.34 PS.seek 1.85ml @20:43:53.70 PBV.dial between PSV and waste1 @20:43:54.08 PS.seek 0ml @20:44:13.67 delay 2 seconds @20:44:15.68 Beginning samplePassC @20:44:15.70 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:44:15.86 Elbow.reconfigure :tight @20:44:15.90 Elbow.seek FlushPuck::Garage @20:44:17.28 Elbow.reconfigure :loose @20:44:17.36 Forearm.seek :garage @20:44:18.50 Hand.close @20:44:19.17 Forearm.seek :retract @20:44:20.22 Elbow.reconfigure :tight @20:44:20.26 Elbow.seek CC @20:44:23.07 Elbow.reconfigure :loose @20:44:23.16 Forearm.reconfigure :tight @20:44:23.20 Forearm.seek CC @20:44:24.43 CC.seek :closed @20:44:28.66 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.79 @20:44:28.74 Hand.open @20:44:29.49 Forearm.reconfigure :loose @20:44:29.53 Forearm.seek :retract @20:44:30.52 PSV.dial PSR @20:44:31.58 Pulling 1.5ml of PSR.flush @20:44:31.59 PSR.select :flush @20:44:31.70 PS.seek 1.5ml @20:44:47.67 delay 5 seconds @20:44:52.68 PSV.dial :waste1 @20:44:53.14 PS.seek 0ml @20:45:09.16 PSV.dial PSR @20:45:09.60 PSR.select :air @20:45:09.71 PS.seek 2.5ml @20:45:35.89 PRV.dial CBV,avoiding: :ab2 @20:45:35.91
CBV.dial PRV @20:45:37.51 PSV.dial :mixloop @20:45:38.51 CTV.dial :air,avoiding: :intake @20:45:38.52 PTV.dial :PRVmixing @20:45:39.72 PS.seek 0ml @20:46:05.91 PSV.dial :air @20:46:06.36 PS.seek 2.5ml @20:46:32.57 PSV.dial :mixloop @20:46:33.03 PS.seek 0ml @20:46:59.22 PSV.dial :air @20:46:59.68 PS.seek 2.5ml @20:47:25.82 PSV.dial :mixloop @20:47:26.26 PS.seek 0ml @20:47:52.51 Garaging FlushPuck in Collection Clamp @20:47:52.58 Elbow.reconfigure :tight @20:47:52.62 Elbow.seek CC @20:47:52.72 Elbow.reconfigure :loose @20:47:52.81 Forearm.seek CC @20:47:53.79 Hand.close @20:47:54.55 CC.seek :open @20:47:58.90 Forearm.seek :retract @20:47:59.90 Elbow.reconfigure :tight @20:47:59.95 Elbow.seek :garage @20:48:03.00 Elbow.reconfigure :loose @20:48:03.09 Forearm.reconfigure :tight @20:48:03.13 Forearm.seek :garage @20:48:04.30 Hand.open @20:48:05.10 Forearm.reconfigure :loose @20:48:05.14 Forearm.seek :retract @20:48:06.22 delayUntil "20:52:15.67" @20:48:06.24 Whole Cell Archive sampling 1000ml @20:48:06.26 Loading wcr puck from tube 3 into Collection Clamp @20:48:06.70 Elbow.reconfigure :tight @20:48:06.75 Elbow.seek 3 @20:48:08.70 Forearm.reconfigure :tight @20:48:08.75 Forearm.seek 3 + 700 counts @20:48:09.78 SE.reconfigure :pick @20:48:09.83 SE.seek :beamBreak @20:48:12.37 SE.configure :default @20:48:12.52 Elbow.seek 3 @20:48:12.96 Forearm.seek 3 + 300 counts @20:48:13.23 Forearm.reconfigure :loose @20:48:13.27 Hand.close @20:48:14.05 SE.seek :down @20:48:14.25 Forearm.seek :retract @20:48:15.24 Elbow.seek CC @20:48:16.22 SE.stop @20:48:16.80 Elbow.reconfigure :loose @20:48:16.90 Forearm.reconfigure :tight @20:48:16.94 Forearm.seek CC @20:48:18.19 CC.seek :closed @20:48:22.44 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.67 @20:48:22.45 Hand.open @20:48:23.23 Forearm.reconfigure :loose @20:48:23.27 Forearm.seek :retract @20:48:24.26 collectSample 1000ml w/bubble point of 35psi @20:48:24.28 Priming 7ml @20:48:24.29 Intake.select :close @20:48:24.30 Exhaust.select :close @20:48:24.31 SS.hold @20:48:24.50 Calibrating pressure sensors @20:48:24.52 CTV.dial :air @20:48:24.53 SSV.dial :spit,avoiding: :exhaust @20:48:24.55 SS.reconfigure :fast @20:48:24.59 SS.seek 5ml @20:48:27.85 SSV.dial CBV @20:48:27.88 CBV.dial SSV @20:48:29.20 delay 3.8 seconds @20:48:33.12 SSV.dial :spit @20:48:34.44 SS.seek 0ml @20:48:37.59 SSV.dial CBV @20:48:38.89 CTV.dial :intake @20:48:39.62 SSV.dial CBV @20:48:39.65 CBV.dial SSV @20:48:39.66 Allowing 40s for 7.0ml of remaining 7.0ml @20:48:39.67 Intake.select :open @20:48:41.83 Intake @15.1psia, vacuum from 9.6 to 12.6psi @20:48:41.85 SS.reconfigure :inhaling 2.14ml/s @20:48:41.89 SS.seek 7ml @20:48:47.65 Stroke completed -- waiting for pressure to equalize ... @20:48:47.67 SS.reconfigure :exhale @20:48:47.71 SS.hold @20:49:14.13 Intake.select :close @20:49:14.27 SSV.dial :exhaust @20:49:15.02 Exhaust.select :open @20:49:15.13 SS.seek 0ml @20:49:19.36 Exhaust.select :close @20:49:19.46 Sampling 1000.0ml @20:49:19.47 Intake.select :close @20:49:19.48 Exhaust.select :close @20:49:19.50 SS.reconfigure :default @20:49:19.53 SS.hold @20:49:19.72 CTV.dial :intake @20:49:19.74 SSV.dial CBV @20:49:19.78 CBV.dial SSV @20:49:20.52 Allowing 130s for 25.0ml of remaining 1000.0ml @20:49:20.53 Intake.select :open @20:49:22.69 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:49:22.70 SS.reconfigure :inhaling 2.14ml/s @20:49:22.75 SS.seek 25ml @20:49:27.69 Vacuum too high -- waiting up to 40 seconds @20:49:27.70 SS.reconfigure :exhale @20:49:27.75 SS.hold @20:49:31.59 Intake @13.9psia, vacuum from 8.4 to 11.4psi @20:49:31.61 SS.reconfigure :inhaling 1.93ml/s @20:49:31.65 SS.seek 25ml @20:49:33.97 Vacuum too high -- waiting up to 40 seconds @20:49:33.98 SS.reconfigure :exhale @20:49:34.03 SS.hold @20:49:36.72 Intake @13.6psia, vacuum from 8.1 to 11.1psi @20:49:36.74 SS.reconfigure :inhaling 1.74ml/s @20:49:36.78 SS.seek 25ml @20:49:38.91 Vacuum too high -- waiting up to 40 seconds @20:49:38.92 SS.reconfigure :exhale @20:49:38.98 SS.hold @20:49:41.18 Intake @13.5psia, vacuum from 8.0 to 11.0psi @20:49:41.20 SS.reconfigure :inhaling 1.57ml/s @20:49:41.24 SS.seek 25ml @20:49:43.44 Vacuum too high -- waiting up to 40 seconds @20:49:43.45 SS.reconfigure :exhale @20:49:43.99 SS.hold @20:49:45.65 Intake @13.8psia, vacuum from 8.3 to 11.3psi @20:49:45.67 SS.reconfigure :inhaling 1.41ml/s @20:49:45.71 SS.seek 25ml @20:49:47.57 Vacuum too high -- waiting up to 40 seconds @20:49:47.58 SS.reconfigure :exhale @20:49:47.62 SS.hold @20:49:49.81 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:49:49.82 SS.reconfigure :inhaling 1.27ml/s @20:49:49.87 SS.seek 25ml @20:49:52.03 Vacuum too high -- waiting up to 40 seconds @20:49:52.05 SS.reconfigure :exhale @20:49:52.09 SS.hold @20:49:53.78 Intake @13.1psia, vacuum from 7.6 to 10.6psi @20:49:53.80 SS.reconfigure :inhaling 1.14ml/s @20:49:53.85 SS.seek 25ml @20:49:55.97 Vacuum too high -- waiting up to 40 seconds @20:49:55.98 SS.reconfigure :exhale @20:49:56.02 SS.hold @20:49:59.04 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:49:59.06 SS.reconfigure :inhaling 1.03ml/s @20:49:59.10 SS.seek 25ml @20:50:01.57 Vacuum too high -- waiting up to 40 seconds @20:50:01.59 SS.reconfigure :exhale @20:50:01.63 SS.hold @20:50:02.29 Intake @12.9psia, vacuum from 7.4 to 10.4psi @20:50:02.31 SS.reconfigure :inhaling 0.93ml/s @20:50:02.36 SS.seek 25ml @20:50:04.19 Vacuum too high -- waiting up to 40 seconds @20:50:04.20 SS.reconfigure :exhale @20:50:04.25 SS.hold @20:50:07.27 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:50:07.28 SS.reconfigure :inhaling 0.84ml/s @20:50:07.33 SS.seek 25ml @20:50:10.10 Vacuum too high -- waiting up to 40 seconds @20:50:10.11 SS.reconfigure :exhale @20:50:10.15 SS.hold @20:50:10.55 Intake @12.8psia, vacuum from 7.3 to 10.3psi @20:50:10.57 SS.reconfigure :inhaling 0.76ml/s @20:50:10.61 SS.seek 25ml @20:50:12.88 Vacuum too high -- waiting up to 40 seconds @20:50:12.89 SS.reconfigure :exhale @20:50:12.93 SS.hold @20:50:15.94 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:50:15.95 SS.reconfigure :inhaling 0.69ml/s @20:50:16.00 SS.seek 25ml @20:50:20.42 Stroke completed -- waiting for pressure to equalize ... @20:50:20.43 SS.reconfigure :exhale @20:50:20.47 SS.hold @20:50:25.33 Intake.select :close @20:50:25.47 SSV.dial :exhaust @20:50:26.70 Exhaust.select :open @20:50:26.81 SS.seek 0ml @20:50:36.20 Exhaust.select :close @20:50:36.31 SSV.dial CBV @20:50:36.33 CBV.dial SSV @20:50:37.09 Allowing 130s for 25.0ml of remaining 975.0ml @20:50:37.10 Intake.select :open @20:50:39.26 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:50:39.28 SS.reconfigure :inhaling 0.69ml/s @20:50:39.32 SS.seek 25ml @20:50:44.16 Vacuum too high -- waiting up to 40 seconds @20:50:44.17 SS.reconfigure :exhale @20:50:44.21 SS.hold @20:50:45.51 Intake @15.2psia, vacuum from 9.7 to 12.7psi @20:50:45.53 SS.reconfigure :inhaling 0.61ml/s @20:50:45.57 SS.seek 25ml @20:50:51.31 Vacuum too high -- waiting up to 40 seconds @20:50:51.33 SS.reconfigure :exhale @20:50:51.37 SS.hold @20:50:52.66 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:50:52.68 SS.reconfigure :inhaling 0.56ml/s @20:50:52.72 SS.seek 25ml @20:51:00.94 Vacuum too high -- waiting up to 40 seconds @20:51:00.95 SS.reconfigure :exhale @20:51:00.99 SS.hold @20:51:02.29 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:51:02.31 SS.reconfigure :inhaling 0.50ml/s @20:51:02.35 SS.seek 25ml @20:51:32.31 Vacuum too high -- waiting up to 40 seconds @20:51:32.32 SS.reconfigure :exhale @20:51:32.37 SS.hold @20:51:34.43 Intake @15.1psia, vacuum from 9.6 to 12.6psi @20:51:34.44 SS.reconfigure :inhaling 0.46ml/s @20:51:34.49 SS.seek 25ml @20:51:35.40 Stroke completed -- waiting for pressure to equalize ... @20:51:35.41 SS.reconfigure :exhale @20:51:35.45 SS.hold @20:51:37.42 Intake.select :close @20:51:37.57 SSV.dial :exhaust @20:51:38.29 Exhaust.select :open @20:51:38.40 SS.seek 0ml @20:51:47.65 Exhaust.select :close @20:51:47.75 SSV.dial CBV @20:51:47.78 CBV.dial SSV @20:51:48.54 Allowing 130s for 25.0ml of remaining 950.0ml @20:51:48.55 Intake.select :open @20:51:50.71 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:51:50.73 SS.reconfigure :inhaling 0.46ml/s @20:51:50.77 SS.seek 25ml @20:52:15.68 recoverPRVreagent08 @20:52:15.69 PTV.dial :mixing @20:52:16.42 PBV.dial :waste1 @20:52:16.80 delay 30 seconds @20:52:46.82 PBV.dial between PSV and waste1 @20:52:47.17 PSV.dial :air @20:52:47.64 PS.reconfigure :fast @20:52:47.68 PS.seek 2.5ml @20:52:48.13 Stroke completed -- waiting for pressure to equalize ... @20:52:48.14 SS.reconfigure :exhale @20:52:48.18 SS.hold @20:52:52.48 Intake.select :close @20:52:52.63 SSV.dial :exhaust @20:52:53.38 Exhaust.select :open @20:52:53.49 SS.seek 0ml @20:53:02.99 Exhaust.select :close @20:53:03.10 SSV.dial CBV @20:53:03.12 CBV.dial SSV @20:53:03.87 Allowing 130s for 25.0ml of remaining 925.0ml @20:53:04.35 Intake.select :open @20:53:06.50 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:53:06.52 SS.reconfigure :inhaling 0.46ml/s @20:53:06.56 SS.seek 25ml @20:53:11.06 PSV.dial :mixloop @20:53:11.53 delay 2 seconds @20:53:13.54 PSV.dial :air @20:53:14.01 delay 2 seconds @20:53:16.03 PSV.dial :mixloop @20:53:16.50 delay 2 seconds @20:53:18.51 PSV.dial :air @20:53:18.99 PS.seek 0ml @20:53:41.75 washCycleDA #1 of 2 @20:53:41.76 PSR.select :air @20:53:41.77 PSV.dial PSR @20:53:43.11 Pulling 0.5ml of PSR.wash @20:53:43.12 PSR.select :wash @20:53:43.24 PS.reconfigure :default @20:53:43.28 PS.seek 0.5ml @20:53:49.15 delay 5 seconds @20:53:54.17 PSR.select :air @20:53:54.29 PS.seek 2ml @20:54:04.27 Stroke completed -- waiting for pressure to equalize ... @20:54:04.28 SS.reconfigure :exhale @20:54:04.32 SS.hold @20:54:09.09 Intake.select :close @20:54:09.23 SSV.dial :exhaust @20:54:09.99 Exhaust.select :open @20:54:10.10 SS.seek 0ml @20:54:10.31 PTV.dial :puck @20:54:11.03 PSV.dial PTV @20:54:11.76 PBV.dial :waste1 @20:54:12.19 PS.seek 1.5ml @20:54:18.12 delay 3 seconds @20:54:19.63 Exhaust.select :close @20:54:19.74 SSV.dial CBV @20:54:19.76 CBV.dial SSV @20:54:20.52 Allowing 130s for 25.0ml of remaining 900.0ml @20:54:20.53 Intake.select :open @20:54:21.13 PBV.dial between PSV and waste1 @20:54:21.50 PS.seek 0ml @20:54:22.69 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:54:22.71 SS.reconfigure :inhaling 0.46ml/s @20:54:22.75 SS.seek 25ml @20:54:37.62 delay 2:00 @20:55:19.83 Stroke completed -- waiting for pressure to equalize ... @20:55:19.84 SS.reconfigure :exhale @20:55:19.88 SS.hold @20:55:25.46 Intake.select :close @20:55:25.60 SSV.dial :exhaust @20:55:26.35 Exhaust.select :open @20:55:26.46 SS.seek 0ml @20:55:35.53 Exhaust.select :close @20:55:35.64 SSV.dial CBV @20:55:35.66 CBV.dial SSV @20:55:36.41 Allowing 130s for 25.0ml of remaining 875.0ml @20:55:36.42 Intake.select :open @20:55:38.58 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:55:38.59 SS.reconfigure :inhaling 0.46ml/s @20:55:38.64 SS.seek 25ml @20:56:15.49 Vacuum too high -- waiting up to 40 seconds @20:56:15.50 SS.reconfigure :exhale @20:56:15.55 SS.hold @20:56:17.60 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:56:17.61 SS.reconfigure :inhaling 0.41ml/s @20:56:17.66 SS.seek 25ml @20:56:37.64 recoverPSRreagent08 @20:56:37.65 PTV.dial :puck @20:56:37.67 PBV.dial :waste1 @20:56:38.02 delay 30 seconds @20:56:41.67 Stroke completed -- waiting for pressure to equalize ... @20:56:41.68 SS.reconfigure :exhale @20:56:41.72 SS.hold @20:56:46.29 Intake.select :close @20:56:46.44 SSV.dial :exhaust @20:56:47.19 Exhaust.select :open @20:56:47.30 SS.seek 0ml @20:56:56.75 Exhaust.select :close @20:56:56.86 SSV.dial CBV @20:56:56.88 CBV.dial SSV @20:56:57.66 Allowing 130s for 25.0ml of remaining 850.0ml @20:56:57.67 Intake.select :open @20:56:59.82 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:56:59.84 SS.reconfigure :inhaling 0.41ml/s @20:56:59.89 SS.seek 25ml @20:57:08.04 PBV.dial between PSV and waste1 @20:57:08.40 PSR.select :air @20:57:08.42 PSV.dial PSR @20:57:09.17 PS.reconfigure :fast @20:57:09.21 PS.seek 2.5ml @20:57:32.21 PSV.dial PTV @20:57:32.94 delay 5 seconds @20:57:37.96 PSV.dial PSR @20:57:38.82 delay 5 seconds @20:57:43.84 PSV.dial PTV @20:57:44.55 delay 5 seconds @20:57:49.57 PSV.dial :air @20:57:50.31 PS.seek 0ml @20:58:02.93 Stroke completed -- waiting for pressure to equalize ... @20:58:02.95 SS.reconfigure :exhale @20:58:02.99 SS.hold @20:58:08.54 Intake.select :close @20:58:08.68 SSV.dial :exhaust @20:58:09.43 Exhaust.select :open @20:58:09.55 SS.seek 0ml @20:58:13.21 washCycleDA #2 of 2 @20:58:13.24 PSR.select :air @20:58:13.26 PSV.dial PSR @20:58:14.62 Pulling 0.5ml of PSR.wash @20:58:14.63 PSR.select :wash @20:58:14.74 PS.reconfigure :default @20:58:14.78 PS.seek 0.5ml @20:58:18.89 Exhaust.select :close @20:58:18.99 SSV.dial CBV @20:58:19.02 CBV.dial SSV @20:58:19.79 Allowing 130s for 25.0ml of remaining 825.0ml @20:58:19.80 Intake.select :open @20:58:20.74 delay 5 seconds @20:58:21.96 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:58:21.98 SS.reconfigure :inhaling 0.41ml/s @20:58:22.02 SS.seek 25ml @20:58:25.76 PSR.select :air @20:58:25.87 PS.seek 2ml @20:58:41.89 PTV.dial :puck @20:58:41.91 PSV.dial PTV @20:58:42.63 PBV.dial :waste1 @20:58:43.05 PS.seek 1.5ml @20:58:48.99 delay 3 seconds @20:58:52.00 PBV.dial between PSV and waste1 @20:58:52.39 PS.seek 0ml @20:59:08.43 delay 2:00 @20:59:25.43 Stroke completed -- waiting for pressure to equalize ... @20:59:25.44 SS.reconfigure :exhale @20:59:25.49 SS.hold @20:59:31.89 Intake.select :close @20:59:32.04 SSV.dial :exhaust @20:59:32.78 Exhaust.select :open @20:59:32.89 SS.seek 0ml @20:59:42.35 Exhaust.select :close @20:59:42.46 SSV.dial CBV @20:59:42.48 CBV.dial SSV @20:59:43.23 Allowing 130s for 25.0ml of remaining 800.0ml @20:59:43.24 Intake.select :open @20:59:45.40 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:59:45.42 SS.reconfigure :inhaling 0.41ml/s @20:59:45.94 SS.seek 25ml @21:00:52.07 Stroke completed -- waiting for pressure to equalize ... @21:00:52.08 SS.reconfigure :exhale @21:00:52.12 SS.hold @21:00:59.50 Intake.select :close @21:00:59.64 SSV.dial :exhaust @21:01:00.38 Exhaust.select :open @21:01:00.49 SS.seek 0ml @21:01:08.45 recoverPSRreagent08 @21:01:08.46 PTV.dial :puck @21:01:08.47 PBV.dial :waste1 @21:01:08.84 delay 30 seconds @21:01:09.90 Exhaust.select :close @21:01:10.00 SSV.dial CBV @21:01:10.03 CBV.dial SSV @21:01:10.79 Allowing 130s for 25.0ml of remaining 775.0ml @21:01:10.80 Intake.select :open @21:01:12.96 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:01:12.98 SS.reconfigure :inhaling 0.41ml/s @21:01:13.02 SS.seek 25ml @21:01:38.86 PBV.dial between PSV and waste1 @21:01:39.24 PSR.select :air @21:01:39.25 PSV.dial PSR @21:01:39.99 PS.reconfigure :fast @21:01:40.03 PS.seek 2.5ml @21:02:03.04 PSV.dial PTV @21:02:03.78 delay 5 seconds @21:02:08.80 PSV.dial PSR @21:02:09.53 delay 5 seconds @21:02:13.26 Vacuum too high -- waiting up to 40 seconds @21:02:13.27 SS.reconfigure :exhale @21:02:13.32 SS.hold @21:02:14.56 PSV.dial PTV @21:02:15.34 delay 5 seconds @21:02:17.49 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:02:17.51 SS.reconfigure :inhaling 0.37ml/s @21:02:17.56 SS.seek 25ml @21:02:20.37 PSV.dial :air @21:02:21.10 PS.seek 0ml @21:02:22.89 Stroke completed -- waiting for pressure to equalize ... @21:02:22.91 SS.reconfigure :exhale @21:02:22.95 SS.hold @21:02:27.71 Intake.select :close @21:02:27.86 SSV.dial :exhaust @21:02:28.60 Exhaust.select :open @21:02:28.71 SS.seek 0ml @21:02:37.74 Exhaust.select :close @21:02:37.84 SSV.dial CBV @21:02:37.87 CBV.dial SSV @21:02:38.63 Allowing 130s for 25.0ml of remaining 750.0ml @21:02:38.64 Intake.select :open @21:02:40.80 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:02:40.82 SS.reconfigure :inhaling 0.37ml/s @21:02:40.86 SS.seek 25ml @21:02:44.11 getPMLReagent08 @21:02:44.12 PRV.dial :air @21:02:44.56 delay 5 seconds @21:02:49.58 PTV.dial :PRVmixing @21:02:50.86 delay 5 seconds @21:02:55.88 PRV.dial :ab2 @21:02:56.32 PTV.dial :PRVmixing @21:02:56.34 PSV.dial :mixloop @21:02:56.78 PS.reconfigure :default @21:02:56.83 PS.seek 0.3ml @21:03:00.73 delay 2 seconds @21:03:02.75 PRV.dial :air @21:03:03.26 PS.seek 0.55ml @21:03:06.61 addPMLReagent08 @21:03:06.62 PSV.dial :air @21:03:07.06 PS.seek 2ml @21:03:22.54 PSV.dial :PML @21:03:23.46 PTV.dial :mixing @21:03:24.21 PBV.dial :waste1 @21:03:24.63 PS.seek 1.85ml @21:03:27.01 PBV.dial between PSV and waste1 @21:03:27.38 PS.seek 0ml @21:03:46.98 delay 2 seconds @21:03:49.00 delay 8:00 @21:03:55.31 Stroke completed -- waiting for pressure to equalize ... @21:03:55.33 SS.reconfigure :exhale @21:03:55.37 SS.hold @21:04:02.76 Intake.select :close @21:04:02.91 SSV.dial :exhaust @21:04:03.66 Exhaust.select :open @21:04:03.77 SS.seek 0ml @21:04:12.86 Exhaust.select :close @21:04:12.97 SSV.dial CBV @21:04:12.99 CBV.dial SSV @21:04:13.77 Allowing 130s for 25.0ml of remaining 725.0ml @21:04:13.78 Intake.select :open @21:04:15.94 Intake @15.1psia, vacuum from 9.6 to 12.6psi @21:04:15.96 SS.reconfigure :inhaling 0.37ml/s @21:04:16.00 SS.seek 25ml @21:05:30.89 Stroke completed -- waiting for pressure to equalize ... @21:05:30.90 SS.reconfigure :exhale @21:05:30.94 SS.hold @21:05:39.44 Intake.select :close @21:05:39.58 SSV.dial :exhaust @21:05:40.33 Exhaust.select :open @21:05:40.44 SS.seek 0ml @21:05:49.92 Exhaust.select :close @21:05:50.03 SSV.dial CBV @21:05:50.05 CBV.dial SSV @21:05:50.81 Allowing 130s for 25.0ml of remaining 700.0ml @21:05:50.83 Intake.select :open @21:05:52.98 Intake @15.0psia, vacuum from 9.5 to 12.5psi @21:05:53.00 SS.reconfigure :inhaling 0.37ml/s @21:05:53.04 SS.seek 25ml @21:07:07.76 Stroke completed -- waiting for pressure to equalize ... @21:07:07.77 SS.reconfigure :exhale @21:07:07.81 SS.hold @21:07:17.49 Intake.select :close @21:07:17.64 SSV.dial :exhaust @21:07:18.39 Exhaust.select :open @21:07:18.50 SS.seek 0ml @21:07:27.93 Exhaust.select :close @21:07:28.04 SSV.dial CBV @21:07:28.06 CBV.dial SSV @21:07:28.81 Allowing 130s for 25.0ml of remaining 675.0ml @21:07:28.82 Intake.select :open @21:07:30.98 Intake @15.2psia, vacuum from 9.7 to 12.7psi @21:07:30.99 SS.reconfigure :inhaling 0.37ml/s @21:07:31.04 SS.seek 25ml @21:08:45.37 Vacuum too high -- waiting up to 40 seconds @21:08:45.39 SS.reconfigure :exhale @21:08:45.43 SS.hold @21:08:50.00 Intake @14.8psia, vacuum from 9.3 to 12.3psi @21:08:50.02 SS.reconfigure :inhaling 0.33ml/s @21:08:50.06 SS.seek 25ml @21:08:52.60 Stroke completed -- waiting for pressure to equalize ... @21:08:52.61 SS.reconfigure :exhale @21:08:52.66 SS.hold @21:08:58.20 Intake.select :close @21:08:58.35 SSV.dial :exhaust @21:08:59.09 Exhaust.select :open @21:08:59.20 SS.seek 0ml @21:09:08.71 Exhaust.select :close @21:09:08.82 SSV.dial CBV @21:09:08.84 CBV.dial SSV @21:09:09.59 Allowing 130s for 25.0ml of remaining 650.0ml @21:09:09.60 Intake.select :open @21:09:11.76 Intake @15.3psia, vacuum from 9.8 to 12.8psi @21:09:11.77 SS.reconfigure :inhaling 0.33ml/s @21:09:11.81 SS.seek 25ml @21:10:38.69 Stroke completed -- waiting for pressure to equalize ... @21:10:38.70 SS.reconfigure :exhale @21:10:38.75 SS.hold @21:10:47.22 Intake.select :close @21:10:47.36 SSV.dial :exhaust @21:10:48.10 Exhaust.select :open @21:10:48.21 SS.seek 0ml @21:10:57.27 Exhaust.select :close @21:10:57.38 SSV.dial CBV @21:10:57.40 CBV.dial SSV @21:10:58.16 Allowing 130s for 25.0ml of remaining 625.0ml @21:10:58.18 Intake.select :open @21:11:00.33 Intake @15.4psia, vacuum from 9.9 to 12.9psi @21:11:00.35 SS.reconfigure :inhaling 0.33ml/s @21:11:00.39 SS.seek 25ml @21:11:49.02 recoverPRVreagent08 @21:11:49.03 PTV.dial :mixing @21:11:49.04 PBV.dial :waste1 @21:11:49.41 delay 30 seconds @21:12:19.43 PBV.dial between PSV and waste1 @21:12:19.78 PSV.dial :air @21:12:20.25 PS.reconfigure :fast @21:12:20.30 PS.seek 2.5ml @21:12:29.94 Stroke completed -- waiting for pressure to equalize ... @21:12:29.95 SS.reconfigure :exhale @21:12:29.99 SS.hold @21:12:39.66 Intake.select :close @21:12:39.81 SSV.dial :exhaust @21:12:40.55 Exhaust.select :open @21:12:40.67 SS.seek 0ml @21:12:43.40 PSV.dial :mixloop @21:12:43.87 delay 2 seconds @21:12:45.89 PSV.dial :air @21:12:46.36 delay 2 seconds @21:12:48.37 PSV.dial :mixloop @21:12:49.33 delay 2 seconds @21:12:49.72 Exhaust.select :close @21:12:49.83 SSV.dial CBV @21:12:49.85 CBV.dial SSV @21:12:50.61 Allowing 130s for 25.0ml of remaining 600.0ml @21:12:50.63 Intake.select :open @21:12:51.34 PSV.dial :air @21:12:51.82 PS.seek 0ml @21:12:52.78 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:12:52.80 SS.reconfigure :inhaling 0.33ml/s @21:12:52.85 SS.seek 25ml @21:13:14.69 washCycleDA #1 of 3 @21:13:14.70 PSR.select :air @21:13:14.71 PSV.dial PSR @21:13:16.05 Pulling 0.5ml of PSR.wash @21:13:16.06 PSR.select :wash @21:13:16.18 PS.reconfigure :default @21:13:16.22 PS.seek 0.5ml @21:13:22.06 delay 5 seconds @21:13:27.08 PSR.select :air @21:13:27.19 PS.seek 2ml @21:13:43.28 PTV.dial :puck @21:13:44.00 PSV.dial PTV @21:13:44.74 PBV.dial :waste1 @21:13:45.17 PS.seek 1.5ml @21:13:51.15 delay 3 seconds @21:13:54.17 PBV.dial between PSV and waste1 @21:13:54.55 PS.seek 0ml @21:14:10.65 delay 2:00 @21:14:23.04 Stroke completed -- waiting for pressure to equalize ... @21:14:23.06 SS.reconfigure :exhale @21:14:23.10 SS.hold @21:14:34.18 Intake.select :close @21:14:34.32 SSV.dial :exhaust @21:14:35.06 Exhaust.select :open @21:14:35.17 SS.seek 0ml @21:14:44.59 Exhaust.select :close @21:14:44.70 SSV.dial CBV @21:14:44.73 CBV.dial SSV @21:14:45.47 Allowing 130s for 25.0ml of remaining 575.0ml @21:14:45.48 Intake.select :open @21:14:47.64 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:14:47.65 SS.reconfigure :inhaling 0.33ml/s @21:14:47.70 SS.seek 25ml @21:16:10.68 recoverPSRreagent08 @21:16:10.69 PTV.dial :puck @21:16:10.70 PBV.dial :waste1 @21:16:11.07 delay 30 seconds @21:16:17.48 Stroke completed -- waiting for pressure to equalize ... @21:16:17.49 SS.reconfigure :exhale @21:16:17.53 SS.hold @21:16:30.09 Intake.select :close @21:16:30.24 SSV.dial :exhaust @21:16:30.98 Exhaust.select :open @21:16:31.09 SS.seek 0ml @21:16:40.59 Exhaust.select :close @21:16:40.69 SSV.dial CBV @21:16:40.72 CBV.dial SSV @21:16:41.09 PBV.dial between PSV and waste1 @21:16:41.44 PSR.select :air @21:16:41.46 PSV.dial PSR @21:16:41.53 Allowing 130s for 25.0ml of remaining 550.0ml @21:16:41.54 Intake.select :open @21:16:42.32 PS.reconfigure :fast @21:16:42.37 PS.seek 2.5ml @21:16:43.69 Intake @15.5psia, vacuum from 10.0 to 13.0psi @21:16:43.71 SS.reconfigure :inhaling 0.33ml/s @21:16:43.75 SS.seek 25ml @21:17:05.49 PSV.dial PTV @21:17:06.21 delay 5 seconds @21:17:11.71 PSV.dial PSR @21:17:12.46 delay 5 seconds @21:17:17.48 PSV.dial PTV @21:17:18.21 delay 5 seconds @21:17:23.23 PSV.dial :air @21:17:23.97 PS.seek 0ml @21:17:46.94 washCycleDA #2 of 3 @21:17:46.95 PSR.select :air @21:17:46.97 PSV.dial PSR @21:17:47.05 Vacuum too high -- waiting up to 40 seconds @21:17:47.06 SS.reconfigure :exhale @21:17:47.10 SS.hold @21:17:48.29 Pulling 0.5ml of PSR.wash @21:17:48.30 PSR.select :wash @21:17:48.48 PS.reconfigure :default @21:17:48.52 PS.seek 0.5ml @21:17:51.41 Intake @15.8psia, vacuum from 10.0 to 13.0psi @21:17:51.43 SS.reconfigure :inhaling 0.30ml/s @21:17:51.47 SS.seek 25ml @21:17:54.46 delay 5 seconds @21:17:59.48 PSR.select :air @21:17:59.59 PS.seek 2ml @21:18:15.64 PTV.dial :puck @21:18:15.66 PSV.dial PTV @21:18:16.38 PBV.dial :waste1 @21:18:16.81 PS.seek 1.5ml @21:18:21.50 Stroke completed -- waiting for pressure to equalize ... @21:18:21.51 SS.reconfigure :exhale @21:18:21.55 SS.hold @21:18:22.67 delay 3 seconds @21:18:25.70 PBV.dial between PSV and waste1 @21:18:26.06 PS.seek 0ml @21:18:32.70 Intake.select :close @21:18:32.84 SSV.dial :exhaust @21:18:33.58 Exhaust.select :open @21:18:33.70 SS.seek 0ml @21:18:42.15 delay 2:00 @21:18:43.16 Exhaust.select :close @21:18:43.27 SSV.dial CBV @21:18:43.30 CBV.dial SSV @21:18:44.04 Allowing 130s for 25.0ml of remaining 525.0ml @21:18:44.05 Intake.select :open @21:18:46.21 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:18:46.23 SS.reconfigure :inhaling 0.30ml/s @21:18:46.27 SS.seek 25ml @21:20:25.27 Stroke completed -- waiting for pressure to equalize ... @21:20:25.28 SS.reconfigure :exhale @21:20:25.32 SS.hold @21:20:39.59 Intake.select :close @21:20:39.73 SSV.dial :exhaust @21:20:40.47 Exhaust.select :open @21:20:40.58 SS.seek 0ml @21:20:42.16 recoverPSRreagent08 @21:20:42.18 PTV.dial :puck @21:20:42.19 PBV.dial :waste1 @21:20:42.55 delay 30 seconds @21:20:50.75 Exhaust.select :close @21:20:50.86 SSV.dial CBV @21:20:50.88 CBV.dial SSV @21:20:51.63 Allowing 130s for 25.0ml of remaining 500.0ml @21:20:51.64 Intake.select :open @21:20:54.32 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:20:54.34 SS.reconfigure :inhaling 0.30ml/s @21:20:54.38 SS.seek 25ml @21:21:12.57 PBV.dial between PSV and waste1 @21:21:12.94 PSR.select :air @21:21:12.96 PSV.dial PSR @21:21:13.71 PS.reconfigure :fast @21:21:13.75 PS.seek 2.5ml @21:21:36.83 PSV.dial PTV @21:21:37.56 delay 5 seconds @21:21:42.58 PSV.dial PSR @21:21:43.31 delay 5 seconds @21:21:48.33 PSV.dial PTV @21:21:49.06 delay 5 seconds @21:21:54.08 PSV.dial :air @21:21:54.82 PS.seek 0ml @21:22:11.82 Vacuum too high -- waiting up to 40 seconds @21:22:11.83 SS.reconfigure :exhale @21:22:11.88 SS.hold @21:22:17.52 Intake @16.7psia, vacuum from 10.0 to 13.0psi @21:22:17.54 SS.reconfigure :inhaling 0.27ml/s @21:22:17.58 SS.seek 25ml @21:22:17.73 washCycleDA #3 of 3 @21:22:17.75 PSR.select :air @21:22:17.76 PSV.dial PSR @21:22:19.08 Pulling 0.5ml of PSR.wash @21:22:19.09 PSR.select :wash @21:22:19.21 PS.reconfigure :default @21:22:19.25 PS.seek 0.5ml @21:22:25.11 delay 5 seconds @21:22:30.13 PSR.select :air @21:22:30.24 PS.seek 2ml @21:22:41.33 Stroke completed -- waiting for pressure to equalize ... @21:22:41.34 SS.reconfigure :exhale @21:22:41.38 SS.hold @21:22:46.29 PTV.dial :puck @21:22:46.30 PSV.dial PTV @21:22:47.03 PBV.dial :waste1 @21:22:47.45 PS.seek 1.5ml @21:22:52.41 Intake.select :close @21:22:52.55 SSV.dial :exhaust @21:22:53.30 Exhaust.select :open @21:22:53.41 SS.seek 0ml @21:22:53.48 delay 3 seconds @21:22:56.50 PBV.dial between PSV and waste1 @21:22:56.88 PS.seek 0ml @21:23:02.93 Exhaust.select :close @21:23:03.04 SSV.dial CBV @21:23:03.09 CBV.dial SSV @21:23:03.80 Allowing 130s for 25.0ml of remaining 475.0ml @21:23:03.81 Intake.select :open @21:23:05.96 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:23:05.98 SS.reconfigure :inhaling 0.27ml/s @21:23:06.03 SS.seek 25ml @21:23:12.92 delay 2:00 @21:24:54.43 Stroke completed -- waiting for pressure to equalize ... @21:24:54.44 SS.reconfigure :exhale @21:24:54.48 SS.hold @21:25:07.04 Intake.select :close @21:25:07.69 SSV.dial :exhaust @21:25:08.45 Exhaust.select :open @21:25:08.56 SS.seek 0ml @21:25:12.94 recoverPSRreagent08 @21:25:12.95 PTV.dial :puck @21:25:12.96 PBV.dial :waste1 @21:25:13.32 delay 30 seconds @21:25:18.03 Exhaust.select :close @21:25:18.14 SSV.dial CBV @21:25:18.17 CBV.dial SSV @21:25:18.92 Allowing 130s for 25.0ml of remaining 450.0ml @21:25:18.93 Intake.select :open @21:25:21.09 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:25:21.11 SS.reconfigure :inhaling 0.27ml/s @21:25:21.15 SS.seek 25ml @21:25:43.35 PBV.dial between PSV and waste1 @21:25:43.71 PSR.select :air @21:25:43.73 PSV.dial PSR @21:25:44.47 PS.reconfigure :fast @21:25:44.51 PS.seek 2.5ml @21:26:07.60 PSV.dial PTV @21:26:08.34 delay 5 seconds @21:26:13.36 PSV.dial PSR @21:26:14.09 delay 5 seconds @21:26:19.11 PSV.dial PTV @21:26:19.84 delay 5 seconds @21:26:24.86 PSV.dial :air @21:26:25.59 PS.seek 0ml @21:26:48.51 Gate.power :camera,:ON @21:26:48.52 Mixing DA Substrates @21:26:48.53 PSR.select :air @21:26:48.54 PSV.dial PSR @21:26:50.12 Pulling 0.25ml of PSR.sub1 @21:26:50.16 PSR.select :sub1 @21:26:50.45 PS.reconfigure :default @21:26:50.63 PS.seek 0.25ml @21:26:54.10 delay 5 seconds @21:26:59.18 Pulling 0.25ml of PSR.sub2 @21:26:59.20 PSR.select :sub2 @21:26:59.31 PS.seek 0.5ml @21:27:02.76 delay 5 seconds @21:27:07.78 PSR.select :air @21:27:07.89 PS.seek 2ml @21:27:08.96 Stroke completed -- waiting for pressure to equalize ... @21:27:08.97 SS.reconfigure :exhale @21:27:09.02 SS.hold @21:27:23.88 PTV.dial :puck @21:27:23.89 PSV.dial PTV @21:27:24.62 PBV.dial :waste1 @21:27:25.04 PS.seek 1.5ml @21:27:25.23 Intake.select :close @21:27:25.38 SSV.dial :exhaust @21:27:26.15 Exhaust.select :open @21:27:26.26 SS.seek 0ml @21:27:31.01 delay 3 seconds @21:27:34.02 PBV.dial between PSV and waste1 @21:27:34.40 PS.seek 0ml @21:27:35.83 Exhaust.select :close @21:27:35.94 SSV.dial CBV @21:27:35.98 CBV.dial SSV @21:27:36.73 Allowing 130s for 25.0ml of remaining 425.0ml @21:27:36.74 Intake.select :open @21:27:38.89 Intake @16.1psia, vacuum from 10.0 to 13.0psi @21:27:38.91 SS.reconfigure :inhaling 0.27ml/s @21:27:38.95 SS.seek 25ml @21:27:50.38 delay 10 seconds @21:28:00.40 recoverPSRreagent08 @21:28:00.41 PTV.dial :puck @21:28:00.42 PBV.dial :waste1 @21:28:00.79 delay 30 seconds @21:28:30.81 PBV.dial between PSV and waste1 @21:28:31.18 PSR.select :air @21:28:31.20 PSV.dial PSR @21:28:31.95 PS.reconfigure :fast @21:28:31.99 PS.seek 2.5ml @21:28:55.44 PSV.dial PTV @21:28:56.18 delay 5 seconds @21:29:01.20 PSV.dial PSR @21:29:01.93 delay 5 seconds @21:29:06.94 PSV.dial PTV @21:29:07.68 delay 5 seconds @21:29:12.70 PSV.dial :air @21:29:13.45 PS.seek 0ml @21:29:21.40 Vacuum too high -- waiting up to 40 seconds @21:29:21.41 SS.reconfigure :exhale @21:29:21.45 SS.hold @21:29:28.29 Intake @16.4psia, vacuum from 10.0 to 13.0psi @21:29:28.31 SS.reconfigure :inhaling 0.24ml/s @21:29:28.35 SS.seek 25ml @21:29:35.73 Stroke completed -- waiting for pressure to equalize ... @21:29:35.75 SS.reconfigure :exhale @21:29:35.79 SS.hold @21:29:36.62 Additional air clear @21:29:36.63 PSR.select :air @21:29:36.65 PSV.dial PSR @21:29:37.99 Pulling 2ml of PSR.air @21:29:38.00 PSR.select :air @21:29:38.03 PS.reconfigure :default @21:29:38.07 PS.seek 2ml @21:29:44.32 Intake.select :close @21:29:44.47 SSV.dial :exhaust @21:29:45.22 Exhaust.select :open @21:29:45.33 SS.seek 0ml @21:29:54.77 Exhaust.select :close @21:29:54.87 SSV.dial CBV @21:29:54.90 CBV.dial SSV @21:29:55.68 Allowing 130s for 25.0ml of remaining 400.0ml @21:29:55.69 Intake.select :open @21:29:57.84 Intake @16.3psia, vacuum from 10.0 to 13.0psi @21:29:57.86 SS.reconfigure :inhaling 0.24ml/s @21:29:57.91 SS.seek 25ml @21:29:59.47 delay 5 seconds @21:30:04.49 PTV.dial :puck @21:30:04.50 PSV.dial PTV @21:30:05.24 PBV.dial :waste1 @21:30:05.67 PS.seek 1.5ml @21:30:11.56 delay 3 seconds @21:30:14.58 PBV.dial between PSV and waste1 @21:30:14.96 delay 1 second @21:30:15.97 PSV.dial :waste1 @21:30:17.05 PS.seek 0ml @21:30:33.08 Processing Puck Heater at 27.9C before imaging @21:30:33.09 PH.coast @21:30:33.22 Imaging da puck in Processing Clamp while storing it to tube 2 @21:30:33.38 SC.reconfigure :tight @21:30:33.43 SC.seek 2 @21:30:37.04 SC.configure :limp @21:30:37.24 Elbow.reconfigure :tight @21:30:37.28 Elbow.seek 2 @21:30:38.95 Forearm.reconfigure :tight @21:30:38.99 Forearm.seek 2 + 700 counts @21:30:40.01 SE.reconfigure :pick @21:30:40.06 SE.seek :beamBreak @21:30:41.87 SE.configure :default @21:30:42.02 Elbow.reconfigure :limp @21:30:42.07 Forearm.reconfigure :loose @21:30:42.11 Forearm.seek 2 @21:30:42.51 Elbow.hold @21:30:42.57 Elbow.reconfigure :tight @21:30:42.66 SE.seek 20.33 Pucks below Top Plate @21:30:43.21 Forearm.force -20 @21:30:43.25 Forearm.seek 2 + 317 counts @21:30:43.56 Forearm.reconfigure :tight @21:30:43.61 Forearm.seek 2 + 700 counts @21:30:43.91 SE.seek 21.33 Pucks below Top Plate @21:30:44.51 Forearm.reconfigure :loose @21:30:44.55 Forearm.seek :retract @21:30:45.48 Elbow.seek PC @21:30:48.03 Elbow.reconfigure :loose @21:30:48.12 Forearm.seek PC @21:30:49.23 Hand.close @21:30:50.47 PC.seek :open @21:30:54.55 Forearm.seek :retract @21:30:55.57 Elbow.reconfigure :tight @21:30:55.62 Elbow.seek 2 @21:30:58.23 Forearm.reconfigure :tight @21:30:58.28 Forearm.seek 2 + 700 counts @21:30:59.30 Hand.open @21:31:00.15 SE.seek 20.97 Pucks below Top Plate @21:31:00.42 SE.reconfigure :seal @21:31:00.47 SE.seek 19.87 Pucks below Top Plate @21:31:01.04 Lights, camera, ACTION! @21:31:01.08 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23mar0821h1000ml.tif @21:31:20.51 Waiting >20 seconds for da to relinquish FlushPuck @21:31:30.26 SX MX5/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25.396 seconds /var/log/esp/da23mar0821h1000ml.tif: TIFF Upload Complete @21:31:30.28 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23mar0821h1000ml15s.tif @21:31:46.06 SX MX5/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23mar0821h1000ml15s.tif: TIFF Upload Complete @21:31:46.08 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da23mar0821h1000ml25s.tif @21:31:59.06 Stroke completed -- waiting for pressure to equalize ... @21:31:59.08 SS.reconfigure :exhale @21:31:59.22 SS.hold @21:32:05.70 SS.reconfigure :default @21:32:05.74 SS.hold @21:32:05.86 Intake.select :close @21:32:06.01 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 625.0ml @21:32:06.02 SSV.dial :exhaust @21:32:06.77 Exhaust.select :open @21:32:06.88 SS.reconfigure :exhale @21:32:06.92 SS.seek 0ml @21:32:11.80 SX MX5/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da23mar0821h1000ml25s.tif: TIFF Upload Complete @21:32:11.81 Gate.power :camera,:OFF @21:32:11.89 DAfans.email "25.396 sec auto-exposure",Subject: "da23mar0821h1000ml images!" @21:32:12.12 Elbow.seek 2 @21:32:12.30 Forearm.seek 2 + 700 counts @21:32:12.51 SE.reconfigure :gently @21:32:12.56 SE.seek 20.58 Pucks below Top Plate @21:32:13.21 Elbow.reconfigure :limp @21:32:13.27 Forearm.reconfigure :loose @21:32:13.31 Forearm.seek 2 @21:32:13.73 Elbow.hold @21:32:13.81 Elbow.reconfigure :tight @21:32:13.86 SE.reconfigure :default @21:32:13.90 SE.seek 20.77 Pucks below Top Plate @21:32:14.40 Forearm.force -20 @21:32:14.44 Forearm.seek 2 + 316 counts @21:32:14.75 Forearm.reconfigure :tight @21:32:14.79 Forearm.seek 2 + 700 counts @21:32:15.15 SE.seek :down @21:32:16.22 Exhaust.select :close @21:32:16.58 Sampled 625.0ml @21:32:16.62 Drying puck before fixing for 20:00 @21:32:16.64 CBV.dial :waste2b @21:32:16.78 SE.stop @21:32:16.82 Forearm.reconfigure :loose @21:32:16.96 Forearm.seek :retract @21:32:17.40 delay 1:00 @21:32:18.13 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @21:32:18.49 Elbow.seek FlushPuck::Garage @21:32:20.52 Elbow.reconfigure :loose @21:32:20.71 Forearm.seek :garage @21:32:22.96 Hand.close @21:32:23.98 Forearm.seek :retract @21:32:25.07 Elbow.reconfigure :tight @21:32:25.14 Elbow.seek PC @21:32:26.65 Elbow.reconfigure :loose @21:32:26.86 Forearm.reconfigure :tight @21:32:26.95 Forearm.seek PC @21:32:28.52 PC.seek :closed @21:32:32.50 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.41 @21:32:32.66 Hand.open @21:32:33.60 Forearm.reconfigure :loose @21:32:33.68 Forearm.seek :retract @21:32:34.71 PSR.select :air @21:32:34.75 PSV.dial PSR @21:32:35.36 Pulling 0.5ml of PSR.flush @21:32:35.38 PSR.select :flush @21:32:35.60 PS.seek 0.5ml @21:32:41.50 delay 5 seconds @21:32:43.74 upsync @21:32:46.53 PSR.select :air @21:32:46.64 PS.seek 2.5ml @21:33:07.81 delay 1 second @21:33:08.83 PRV.dial :waste2,avoiding: :ab2 @21:33:10.65 PTV.dial :PRVmixing @21:33:11.90 PSV.dial :mixloop @21:33:12.92 PS.seek :empty @21:33:17.43 CSV.dial :air @21:33:17.89 CS.reconfigure :fast @21:33:17.93 CS.seek 10ml @21:33:39.15 PSR.select :air @21:33:39.17 PSV.dial PSR @21:33:40.36 Pulling 0.5ml of PSR.flush @21:33:40.37 PSR.select :flush @21:33:40.49 PS.seek 0.5ml @21:33:40.76 Pumping wcr puck from tube 3 up to 35psi... @21:33:40.77 CBV.dial SSV @21:33:42.06 CBV.dial between CSV and waste2b @21:33:43.00 CSV.dial between air and CTV @21:33:43.33 CS.seek 7.027ml @21:33:46.37 delay 5 seconds @21:33:50.63 CTV.dial CSV,avoiding: :air @21:33:50.66 CSV.dial CTV @21:33:51.39 PSR.select :air @21:33:51.47 CS.reconfigure :pressurize @21:33:51.52 CS.seek 0ml @21:33:51.58 PS.seek 2.5ml @21:34:03.62 CBV.dial :waste2b @21:34:03.96 CS.reconfigure :default @21:34:04.01 CS.seek 0ml @21:34:10.79 delay 1:00 @21:34:12.75 delay 1 second @21:34:13.76 PBV.dial :waste1 @21:34:14.14 PTV.dial :mixing @21:34:14.85 PSV.dial :mixloop @21:34:15.87 PS.seek :empty @21:34:42.06 PSV.dial PSR @21:34:43.07 PSR.select :flush @21:34:43.19 PS.seek 1ml @21:34:54.12 PSR.select :air @21:34:54.23 PS.seek 2.5ml @21:35:10.34 PSV.dial PTV @21:35:10.80 CTV.dial :air,avoiding: :intake @21:35:11.07 PTV.dial :puck @21:35:11.79 PBV.dial :waste1 @21:35:11.81 PS.seek 0ml @21:35:12.10 Fix #1 of 2 (with RNAlater) for 10:00 @21:35:12.12 CSV.dial CSR @21:35:12.60 Pulling 2ml of CSR.RNAlater @21:35:12.61 CSR.select :RNAlater @21:35:12.72 CS.seek 2ml @21:35:18.53 delay 5 seconds @21:35:23.55 CSR.select :air @21:35:23.67 CS.reconfigure :fast @21:35:23.71 CS.seek 10ml @21:35:37.99 PSV.dial PSR @21:35:38.73 PSR.select :flush @21:35:38.85 PS.seek 0.5ml @21:35:42.56 Pumping wcr puck from tube 3 up to 35psi... @21:35:42.58 CBV.dial SSV @21:35:43.87 CBV.dial between CSV and waste2b @21:35:44.76 PSR.select :air @21:35:44.88 CSV.dial between air and CTV @21:35:44.94 PS.seek 2.5ml @21:35:45.48 CS.seek 6.946ml @21:35:52.97 CTV.dial CSV,avoiding: :air @21:35:53.00 CSV.dial CTV @21:35:54.33 CS.reconfigure :pressurize @21:35:54.38 CS.seek 0ml @21:36:06.05 PSV.dial PTV @21:36:06.77 PBV.dial PSV @21:36:07.33 PS.seek 0ml @21:36:10.33 delay 10:00 @21:36:33.58 PSV.dial PBV @21:36:34.05 PS.seek 2.5ml @21:37:00.27 PSV.dial :waste1 @21:37:01.00 PS.seek 0ml @21:37:27.18 PSV.dial PSR @21:37:27.63 PSR.select :air @21:37:27.65 PS.seek 2.5ml @21:37:53.86 PSV.dial PTV @21:37:54.58 PBV.dial PSV @21:37:54.61 PS.seek 0ml @21:38:20.86 PSV.dial PBV @21:38:21.32 PS.seek 2.5ml @21:38:47.46 PSV.dial :waste1 @21:38:48.18 PS.seek 0ml @21:39:14.40 PSV.dial PSR @21:39:14.85 PSR.select :flush @21:39:14.96 PS.seek 1ml @21:39:25.95 PSR.select :air @21:39:26.07 PS.seek 2.5ml @21:39:42.08 PSV.dial PTV @21:39:42.80 PTV.dial :puck @21:39:42.81 PBV.dial :waste1 @21:39:43.35 PS.seek 0ml @21:40:09.48 PSV.dial PSR @21:40:10.22 PSR.select :flush @21:40:10.34 PS.seek 0.5ml @21:40:16.26 PSR.select :air @21:40:16.38 PS.seek 2.5ml @21:40:37.52 PSV.dial PTV @21:40:38.25 PBV.dial PSV @21:40:38.80 PS.seek 0ml @21:41:05.01 PSV.dial PBV @21:41:05.48 PS.seek 2.5ml @21:41:31.61 PSV.dial :waste1 @21:41:32.33 PS.seek 0ml @21:41:58.51 PSV.dial PSR @21:41:58.97 PSR.select :air @21:41:58.99 PS.seek 2.5ml @21:42:25.23 PSV.dial PTV @21:42:25.95 PBV.dial PSV @21:42:25.97 PS.seek 0ml @21:42:52.17 PSV.dial PBV @21:42:52.63 PS.seek 2.5ml @21:43:18.82 PSV.dial :waste1 @21:43:19.55 PS.seek 0ml @21:43:45.73 Garaging FlushPuck in Processing Clamp @21:43:45.80 Elbow.reconfigure :tight @21:43:45.85 Elbow.seek PC @21:43:45.96 Elbow.reconfigure :loose @21:43:46.05 Forearm.seek PC @21:43:47.01 Hand.close @21:43:47.69 PC.seek :open @21:43:51.66 Forearm.seek :retract @21:43:52.77 Elbow.reconfigure :tight @21:43:52.81 Elbow.seek :garage @21:43:54.13 Elbow.reconfigure :loose @21:43:54.69 Forearm.reconfigure :tight @21:43:54.73 Forearm.seek :garage @21:43:55.86 Hand.open @21:43:56.63 Forearm.reconfigure :loose @21:43:56.67 Forearm.seek :retract @21:46:10.35 CBV.dial :waste2b @21:46:10.68 CS.reconfigure :default @21:46:10.72 CS.seek 0ml @21:46:13.42 delay 1:00 @21:47:13.45 CTV.dial :air,avoiding: :intake @21:47:14.74 Fix #2 of 2 (with RNAlater) for 10:00 @21:47:14.75 CSV.dial CSR @21:47:15.24 Pulling 1.999ml of CSR.RNAlater @21:47:15.25 CSR.select :RNAlater @21:47:15.37 CS.seek 2ml @21:47:21.30 delay 5 seconds @21:47:26.32 CSR.select :air @21:47:26.43 CS.reconfigure :fast @21:47:26.48 CS.seek 10ml @21:47:44.70 Pumping wcr puck from tube 3 up to 35psi... @21:47:44.72 CBV.dial SSV @21:47:46.00 CBV.dial between CSV and waste2b @21:47:46.95 CSV.dial between air and CTV @21:47:47.55 CS.seek 6.859ml @21:47:55.30 CTV.dial CSV,avoiding: :air @21:47:55.33 CSV.dial CTV @21:47:56.66 CS.reconfigure :pressurize @21:47:56.71 CS.seek 0ml @21:48:12.50 delay 10:00 @21:58:12.52 CBV.dial :waste2b @21:58:12.85 CS.reconfigure :default @21:58:12.89 CS.seek 0ml @21:58:15.59 delay 1:00 @21:59:15.62 CTV.dial :air,avoiding: :intake @21:59:16.95 Storing wcr puck in Collection Clamp to tube 2 @21:59:17.11 SC.reconfigure :tight @21:59:17.16 SC.seek 2 @21:59:17.21 SC.configure :limp @21:59:17.41 Elbow.reconfigure :tight @21:59:17.46 Elbow.seek 2 @21:59:19.43 Forearm.reconfigure :tight @21:59:19.47 Forearm.seek 2 + 700 counts @21:59:20.45 SE.reconfigure :pick @21:59:20.51 SE.seek :beamBreak @21:59:22.34 SE.configure :default @21:59:22.48 Elbow.reconfigure :limp @21:59:22.53 Forearm.reconfigure :loose @21:59:22.57 Forearm.seek 2 @21:59:22.95 Elbow.hold @21:59:23.02 Elbow.reconfigure :tight @21:59:23.12 SE.seek 21.33 Pucks below Top Plate @21:59:23.63 Forearm.force -20 @21:59:23.66 Forearm.seek 2 + 319 counts @21:59:23.98 Forearm.reconfigure :tight @21:59:24.03 Forearm.seek 2 + 700 counts @21:59:24.34 SE.seek 22.33 Pucks below Top Plate @21:59:24.92 Forearm.reconfigure :loose @21:59:24.97 Forearm.seek :retract @21:59:25.90 Elbow.seek CC @21:59:27.45 Elbow.reconfigure :loose @21:59:27.54 Forearm.seek CC @21:59:28.56 Hand.close @21:59:29.29 CC.seek :open @21:59:34.17 Forearm.seek :retract @21:59:35.21 Elbow.reconfigure :tight @21:59:35.26 Elbow.seek 2 @21:59:36.97 Elbow.reconfigure :limp @21:59:37.02 Forearm.seek 2 @21:59:38.13 Elbow.hold @21:59:38.20 Elbow.reconfigure :tight @21:59:38.24 Hand.open @21:59:39.15 Forearm.force -20 @21:59:39.19 Forearm.seek 2 + 320 counts @21:59:39.54 Forearm.reconfigure :tight @21:59:39.59 Forearm.seek 2 + 700 counts @21:59:39.85 SE.seek :down @21:59:40.98 Forearm.reconfigure :loose @21:59:41.03 Forearm.seek :retract @21:59:41.09 SE.stop @21:59:42.02
Starting finalPurge @21:59:42.03 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:59:42.21 Elbow.seek FlushPuck::Garage @21:59:43.96 Elbow.reconfigure :loose @21:59:44.06 Forearm.seek :garage @21:59:45.17 Hand.close @21:59:46.02 Forearm.seek :retract @21:59:47.03 Elbow.reconfigure :tight @21:59:47.08 Elbow.seek CC @21:59:49.88 Elbow.reconfigure :loose @21:59:49.97 Forearm.reconfigure :tight @21:59:50.02 Forearm.seek CC @21:59:51.25 CC.seek :closed @21:59:55.48 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.81 @21:59:55.55 Hand.open @21:59:56.34 Forearm.reconfigure :loose @21:59:56.38 Forearm.seek :retract @21:59:57.35 Flushing the Collection Valve::Manifold @21:59:57.46 CSV.dial CSR @21:59:57.95 Pulling 5ml of CSR.flush @21:59:57.96 CSR.select :flush @21:59:58.08 CS.seek 5ml @22:00:11.53 delay 5 seconds @22:00:16.55 CSR.select :air @22:00:16.69 CS.reconfigure :fast @22:00:16.74 CS.seek 7.5ml @22:00:23.15 CSV.dial :waste @22:00:24.42 CS.reconfigure :default @22:00:24.46 CS.seek 0ml @22:00:44.15 Flushing lower manifold's waste1 port @22:00:44.27 CSV.dial :flush @22:00:44.83 CS.reconfigure :fast @22:00:44.87 CS.seek 5ml @22:00:56.62 delay 5 seconds @22:01:01.64 CSV.dial :air @22:01:02.15 CS.seek 6.999ml @22:01:07.44 CSV.dial CTV @22:01:07.46
CTV.dial CSV @22:01:08.84 CBV.dial :waste1 @22:01:09.58 CS.seek 0ml @22:01:25.74 Flushing lower manifold's waste2 port @22:01:25.86 CSV.dial :flush @22:01:26.58 CS.seek 5ml @22:01:38.34 delay 5 seconds @22:01:43.35 CSV.dial :air @22:01:43.87 CS.seek 7ml @22:01:49.15 CSV.dial CTV @22:01:49.18
CTV.dial CSV @22:01:49.62 CBV.dial :waste2 @22:01:50.65 CS.seek 0ml @22:02:06.74 Flushing lower manifold's waste2b port @22:02:06.86 CSV.dial :flush @22:02:08.06 CS.seek 5ml @22:02:19.83 delay 5 seconds @22:02:24.85 CSV.dial :air @22:02:25.36 CS.seek 6.999ml @22:02:30.65 CSV.dial CTV @22:02:30.67
CTV.dial CSV @22:02:31.12 CBV.dial :waste2b @22:02:32.14 CS.seek 0ml @22:02:48.15 Disinfecting external Intake with kill @22:02:48.26 CSV.dial CSR @22:02:48.75 Pulling 8ml of CSR.kill @22:02:48.76 CSR.select :kill @22:02:48.87 CS.seek 8ml @22:03:07.15 delay 5 seconds @22:03:12.17 CSR.select :air @22:03:12.28 CS.seek 10ml @22:03:17.50 CSV.dial CBV @22:03:17.52
CBV.dial CSV @22:03:18.57 CTV.dial :intake @22:03:19.30 Intake.select :open @22:03:19.41 CS.seek 2ml @22:03:37.55 Intake.select :close @22:03:37.65 CTV.dial :air @22:03:38.40 CS.reconfigure :default @22:03:38.44 CS.seek 0ml @22:03:44.30 CSV.dial CSR @22:03:45.35 Pulling 5ml of CSR.flush @22:03:45.36 CSR.select :flush @22:03:45.48 CS.seek 5ml @22:03:58.86 delay 5 seconds @22:04:03.88 CSR.select :air @22:04:03.99 CS.seek 10ml @22:04:17.36 CSV.dial CBV @22:04:18.35 CS.seek 0ml @22:04:44.46 Garaging FlushPuck in Collection Clamp @22:04:44.53 Elbow.reconfigure :tight @22:04:44.57 Elbow.seek CC @22:04:44.67 Elbow.reconfigure :loose @22:04:44.76 Forearm.seek CC @22:04:45.72 Hand.close @22:04:46.40 CC.seek :open @22:04:50.75 Forearm.seek :retract @22:04:51.74 Elbow.reconfigure :tight @22:04:51.79 Elbow.seek :garage @22:04:54.75 Elbow.reconfigure :loose @22:04:54.84 Forearm.reconfigure :tight @22:04:54.88 Forearm.seek :garage @22:04:56.04 Hand.open @22:04:56.83 Forearm.reconfigure :loose @22:04:56.87 Forearm.seek :retract @22:04:57.91 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 19:27:48 @22:04:57.96 Asleep after completing DA_WCR_030823 assays @22:04:57.98 GoodNews.email "{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@21:56:56, 17.5C, 53% humidity, 15.5psia, 13.948V, 0.331A, 0.324A avg, 4.61W\nSampled 625.0ml",Subject: "Asleep after completing DA_WCR_030823 assays" @22:05:02.68 upsync @17:10:56.91UTC15-Mar-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @17:10:59.44 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @17:11:03.35 Polling Can every 10:00 @17:11:03.46 Cleanly exited at 16:54:58UTC15-Mar-23 @17:11:03.50 Loading /home/esp/esp2/mission/DA_WCR_031523.rb mission @17:11:03.53 CTD.latitude = 36.8044 Degrees North in seawater @17:11:03.54 Initializing Sensors @17:11:03.61
Preparing to sleep until + 00:00:05 @17:11:03.63 GoodNews.email "Starting DA_WCR_031523 w/pucks in tube 3\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:11:03, 17.5C, 52% humidity, 15.5psia, 13.977V, 0.251A, 0.251A avg, 3.51W\nEnding 12:00UTC24-Mar-23",Subject: "DA_WCR_031523 begun" @17:11:03.74
Asleep til + 00:00:05 @17:11:03.75 Continuing at 17:11 @17:11:04.04 Gate.power :core,:OFF @17:11:04.05 Gate.power :raw,:OFF @17:11:04.06 Shutdown @17:11:06.19 CTD.stopLogging @17:11:06.73 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_031523.rb:14\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:11:03, 17.5C, 52% humidity, 15.5psia, 13.977V, 0.251A, 0.251A avg, 3.51W",Subject: "Asleep until 17:11" @17:11:08.12 upsync @17:11:08.52
Powering core bus @17:11:10.71 upsync @17:11:10.89 CTD.setTime 17:11:08 @17:11:11.30 CTD.pumpMode= :duringSample @17:11:14.67 Sampling/Uploading CTD every 30:00/5:00:00 @17:11:16.79
Gate.power :raw,:ON @17:11:17.55 CS.configure :default @17:11:17.64 PS.configure :default @17:11:17.73 SS.configure :default @17:11:17.82 CC.configure :default @17:11:17.91 PC.configure :default @17:11:18.02 Forearm.configure :loose @17:11:18.10 Elbow.configure :loose @17:11:18.21 SC.configure :loose @17:11:18.30 SE.configure :default @17:11:18.34 CH.coast @17:11:18.39 CH.configure :default @17:11:18.41 PH.coast @17:11:18.47 PH.configure :default @17:11:18.50 All dwarf controllers running firmware version 3.85 @17:11:18.61 Valve.reset @17:11:18.81 Intake.select :close @17:11:18.86 Exhaust.select :close @17:11:18.90 CTV.dial :air @17:11:19.00 PSV.dial :air @17:11:19.10 CSV.dial :waste1 @17:11:19.13
Forearm.reconfigure :loose @17:11:19.20 Forearm.home.seek :retract @17:11:19.57 SSV.dial :spit,avoiding: :exhaust @17:11:20.02 Hand.close @17:11:20.05
Elbow.jog 1500 @17:11:20.15 PS.reconfigure :default @17:11:20.20 PS.home.seek 0ml @17:11:20.37 CS.reconfigure :fast @17:11:20.41 SS.reconfigure :default @17:11:20.49 CS.home.seek 0ml @17:11:20.52 SS.home.seek 0ml @17:11:20.66
Elbow.reconfigure :loose @17:11:20.70 Elbow.home.seek :clear @17:11:23.89 SE.reconfigure :default @17:11:23.93 SE.home.seek :down @17:11:24.53 SC.reconfigure :tight @17:11:24.57 SC.home.seek :initial @17:11:28.82 PS.stop @17:11:38.97 CTD@17:11:32, 4.7672C, -0.151m, 31.127psu, 0.38mg/m^3 Can@17:11:08, 17.4C, 52% humidity, 15.5psia, 14.080V, 0.080A, 0.073A avg, 1.12W @17:11:54.62 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @17:11:54.63
Retry #1 of 3 @17:11:54.69 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @17:11:59.07 SC.configure :limp @17:11:59.12 Forearm.seek :retract @17:11:59.17 Elbow.seek :hammer @17:12:00.27 Forearm.reconfigure :tight @17:12:00.31 Forearm.seek retracted @17:12:00.72 CC.reconfigure :default @17:12:00.75 PC.reconfigure :default @17:12:00.82
CC.home.seek :open @17:12:00.86 PC.home.seek :open @17:12:04.12 Notice.email "At 17:12:04 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_031523.rb:14\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD@17:11:32, 4.7672C, -0.151m, 31.127psu, 0.38mg/m^3\nCan@17:12:04, 17.5C, 52% humidity, 15.5psia, 13.972V, 0.245A, 0.245A avg, 3.42W",Subject: "Started DA_WCR_031523 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @17:12:04.16
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @17:12:04.17 Starting initialPurge @17:12:04.18 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:12:04.25 Hand.open @17:12:05.11 Elbow.reconfigure :tight @17:12:05.17 Elbow.seek FlushPuck::Garage @17:12:07.23 Elbow.reconfigure :loose @17:12:07.35 Forearm.reconfigure :loose @17:12:07.40 Forearm.seek :garage @17:12:08.22 upsync @17:12:08.50
Hand.close @17:12:09.36 Forearm.seek :retract @17:12:10.40 Elbow.reconfigure :tight @17:12:10.45 Elbow.seek CC @17:12:13.23 Elbow.reconfigure :loose @17:12:13.32 Forearm.reconfigure :tight @17:12:13.37 Forearm.seek CC @17:12:14.64 CC.seek :closed @17:12:18.86 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.82 @17:12:18.93 Hand.open @17:12:19.67 Forearm.reconfigure :loose @17:12:19.71 Forearm.seek :retract @17:12:20.69 Flushing the Collection Valve::Manifold @17:12:20.81 CSV.dial CSR @17:12:22.14 Pulling 4.999ml of CSR.flush CSR.select :flush @17:12:22.26 CS.reconfigure :default @17:12:22.30 CS.seek 5ml @17:12:35.77 delay 5 seconds @17:12:40.79 CSR.select :air @17:12:40.94 CS.reconfigure :fast @17:12:40.98 CS.seek 7.5ml @17:12:47.36 CSV.dial :waste @17:12:48.74 CS.reconfigure :default @17:12:48.78 CS.seek 0ml @17:13:08.55 Purging external Intake @17:13:08.66 CSV.dial :flush @17:13:09.13 CS.reconfigure :fast @17:13:09.17 CS.seek 10ml @17:13:32.11 delay 5 seconds @17:13:37.13 CSV.dial CBV @17:13:37.16
CBV.dial CSV @17:13:37.91 CTV.dial :intake @17:13:38.64 Intake.select :open @17:13:38.75 CS.seek 0ml @17:14:01.42 Intake.select :close @17:14:01.52 CTV.dial :air @17:14:02.27 Flushing upper manifold @17:14:02.39 CSV.dial :flush @17:14:03.12 CS.seek 5ml @17:14:15.02 delay 5 seconds @17:14:20.03 CSV.dial :air @17:14:20.53 CS.seek 7ml @17:14:25.80 CSV.dial CBV @17:14:25.82
CBV.dial CSV @17:14:26.97 CTV.dial :air @17:14:26.99 CS.seek 0ml @17:14:43.08 delay 7 seconds @17:14:50.10 CS.seek 3ml @17:14:57.61 CSV.dial :waste @17:14:58.04 CS.seek 0ml @17:15:05.62 CSV.dial :flush @17:15:06.06 CS.seek 3ml @17:15:13.52 delay 5 seconds @17:15:18.53 CSV.dial :air @17:15:19.03 CS.seek 4.999ml @17:15:24.33 CSV.dial :air @17:15:24.35 CS.seek 0ml @17:15:36.17 delay 7 seconds @17:15:43.20 CS.seek 3ml @17:15:50.67 CSV.dial :waste @17:15:51.39 CS.seek 0ml @17:15:58.80 Garaging FlushPuck in Collection Clamp @17:15:58.87 Elbow.reconfigure :tight @17:15:58.91 Elbow.seek CC @17:15:59.01 Elbow.reconfigure :loose @17:15:59.11 Forearm.seek CC @17:16:00.04 Hand.close @17:16:00.73 CC.seek :open @17:16:05.06 Forearm.seek :retract @17:16:06.02 Elbow.reconfigure :tight @17:16:06.07 Elbow.seek :garage @17:16:09.12 Elbow.reconfigure :loose @17:16:09.22 Forearm.reconfigure :tight @17:16:09.26 Forearm.seek :garage @17:16:10.44 Hand.open @17:16:11.66 Forearm.reconfigure :loose @17:16:11.70 Forearm.seek :retract @17:16:12.74 da sampling at most 1000ml, wcr at most 1000ml @17:16:12.79 Loading sh1 puck from tube 3 into Collection Clamp @17:16:12.81 DAfans.email "Started da in tube 3 at 17:16:12UTC15-Mar-23\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD@17:11:32, 4.7672C, -0.151m, 31.127psu, 0.38mg/m^3\nCan@17:12:04, 17.5C, 52% humidity, 15.5psia, 13.972V, 0.245A, 0.245A avg, 3.42W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @17:16:13.06
SC.reconfigure :tight @17:16:13.13 SC.seek 3 @17:16:16.72 upsync @17:16:23.01
SC.configure :limp @17:16:23.22 Elbow.reconfigure :tight @17:16:23.27 Elbow.seek 3 @17:16:25.19 Forearm.reconfigure :tight @17:16:25.24 Forearm.seek 3 + 700 counts @17:16:26.28 SE.reconfigure :pick @17:16:26.33 SE.seek :beamBreak @17:16:28.89 SE.configure :default @17:16:29.04 Elbow.seek 3 @17:16:29.22 Forearm.seek 3 + 300 counts @17:16:29.52 Forearm.reconfigure :loose @17:16:29.56 Hand.close @17:16:30.37 SE.seek :down @17:16:30.55
Forearm.seek :retract @17:16:31.49 Elbow.seek CC @17:16:32.55 SE.stop @17:16:33.12
Elbow.reconfigure :loose @17:16:33.21 Forearm.reconfigure :tight @17:16:33.25 Forearm.seek CC @17:16:34.55 CC.seek :closed @17:16:38.78 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.67 @17:16:38.79 Hand.open @17:16:39.54 Forearm.reconfigure :loose @17:16:39.58 Forearm.seek :retract @17:16:40.56 collectSample 1000ml w/bubble point of 24psi @17:16:40.57 Priming 7ml @17:16:40.58 Intake.select :close @17:16:40.59 Exhaust.select :close @17:16:40.60 SS.hold @17:16:40.79 Calibrating pressure and temperature sensors @17:16:40.82 CTV.dial :air @17:16:40.84 SSV.dial :spit,avoiding: :exhaust @17:16:40.85 SS.reconfigure :fast @17:16:40.90 SS.seek 5ml @17:16:44.10 SSV.dial CBV @17:16:44.13
CBV.dial SSV @17:16:45.46 delay 3.8 seconds @17:16:49.38 SSV.dial :spit @17:16:50.67 SS.seek 0ml @17:16:53.51 SSV.dial CBV @17:16:54.82 CTV.dial :intake @17:16:55.56 SSV.dial CBV @17:16:55.58
CBV.dial SSV @17:16:55.60 Allowing 40s for 7.0ml of remaining 7.0ml @17:16:55.61 Intake.select :open @17:16:57.76 Intake @14.8psia, vacuum from 9.3 to 12.3psi @17:16:57.77 SS.reconfigure :inhaling 2.14ml/s @17:16:57.82 SS.seek 7ml @17:17:03.56 Stroke completed -- waiting for pressure to equalize ... @17:17:03.57 SS.reconfigure :exhale @17:17:03.61 SS.hold @17:17:03.91 Intake.select :close @17:17:04.05 SSV.dial :exhaust @17:17:04.79 Exhaust.select :open @17:17:04.90 SS.seek 0ml @17:17:09.23 Exhaust.select :close @17:17:09.34 Sampling 1000.0ml @17:17:09.35 SensorPolling.stop @17:17:09.38
Intake.select :close @17:17:09.40 Exhaust.select :close @17:17:09.43 SS.reconfigure :default @17:17:09.46 SS.hold @17:17:09.65 CTV.dial :intake @17:17:09.67 SSV.dial CBV @17:17:09.70
CBV.dial SSV @17:17:10.45 Allowing 130s for 25.0ml of remaining 1000.0ml @17:17:10.48 Intake.select :open @17:17:12.65 Intake @14.9psia, vacuum from 9.4 to 12.4psi @17:17:12.66 SS.reconfigure :inhaling 2.14ml/s @17:17:12.71 SS.seek 25ml @17:17:15.01 CTD.update 6489 samples @17:17:18.04
Vacuum too high -- waiting up to 40 seconds @17:17:18.05 SS.reconfigure :exhale @17:17:18.10 SS.hold @17:17:18.81 Intake @10.0psia, vacuum from 4.5 to 7.5psi @17:17:18.83 SS.reconfigure :inhaling 1.93ml/s @17:17:18.87 SS.seek 25ml @17:17:21.10 Vacuum too high -- waiting up to 40 seconds @17:17:21.11 SS.reconfigure :exhale @17:17:21.17 SS.hold @17:17:22.11 Intake @13.6psia, vacuum from 8.1 to 11.1psi @17:17:22.13 SS.reconfigure :inhaling 1.74ml/s @17:17:22.18 SS.seek 25ml @17:17:25.59 Vacuum too high -- waiting up to 40 seconds @17:17:25.60 SS.reconfigure :exhale @17:17:25.64 SS.hold @17:17:25.90 Intake @12.4psia, vacuum from 6.9 to 9.9psi @17:17:25.92 SS.reconfigure :inhaling 1.57ml/s @17:17:25.97 SS.seek 25ml @17:17:28.63 Vacuum too high -- waiting up to 40 seconds @17:17:28.65 SS.reconfigure :exhale @17:17:28.70 SS.hold @17:17:34.14 I2C::Bus::Error in core_reader -- Timeout @17:17:40.98 I2C::Gateway::Port::Error in core_reader -- No Response to BREAK @17:17:40.99 Retry #1 of 3 @17:17:47.82 I2C::Gateway::Port::Error in core_reader -- No Response to BREAK @17:17:47.83 Retry #2 of 3 @17:17:54.65 I2C::Gateway::Port::Error in core_reader -- No Response to BREAK @17:17:54.66 Retry #3 of 3 @17:18:01.48 Try 'Gate.reconnect' to recover @17:19:20.23 BadNews.email "Could not get prompt from CTD on ctd",Subject: "Instrument::ReadTimeout in pollContext" @17:19:20.32 Instrument Sampling will Restart after 30:00 @17:19:20.33 delay 30:00 @17:19:20.48
SS.reconfigure :default @17:19:24.42 upsync @17:19:56.20 Session Begun @17:20:13.99 -> Gate.reconnect @17:20:20.83 I2C::Gateway::Port::Error in core_reader -- No Response to BREAK @17:20:20.84 Retry #1 of 3 @17:20:27.67 I2C::Gateway::Port::Error in core_reader -- No Response to BREAK @17:20:27.68 Retry #2 of 3 @17:20:34.51 I2C::Gateway::Port::Error in core_reader -- No Response to BREAK @17:20:34.52 Retry #3 of 3 @17:20:41.35 Try 'Gate.reconnect' to recover @17:47:06.66 -> Gate.reconnect @17:47:13.50 I2C::Gateway::Port::Error in core_reader -- No Response to BREAK @17:47:13.52 Retry #1 of 3 @17:47:20.34 I2C::Gateway::Port::Error in core_reader -- No Response to BREAK @17:47:20.35 Retry #2 of 3 @17:47:27.18 I2C::Gateway::Port::Error in core_reader -- No Response to BREAK @17:47:27.19 Retry #3 of 3 @17:47:34.02 Try 'Gate.reconnect' to recover @17:49:45.42 Instrument::ReadTimeout in pollContext -- Could not get prompt from CTD on ctd @17:49:45.46 BadNews.email "Could not get prompt from CTD on ctd",Subject: "Instrument::ReadTimeout in pollContext" @17:49:45.50 Sampling/Uploading CTD every 30:00/30:00 @17:49:49.72 upsync @17:50:10.62 Instrument::ReadTimeout in pollContext -- Could not get prompt from CTD on ctd @17:50:35.72 BadNews.email "Could not get prompt from CTD on ctd",Subject: "Instrument::ReadTimeout in pollContext" @17:50:35.76 Instrument Sampling will Restart after 30:00 @17:50:35.79 delay 30:00 @17:50:39.65 upsync @18:21:00.88 Instrument::ReadTimeout in pollContext -- Could not get prompt from CTD on ctd @18:21:00.91 BadNews.email "Could not get prompt from CTD on ctd",Subject: "Instrument::ReadTimeout in pollContext" @18:21:05.15 upsync @19:29:21.41 -> Gate.reconnect @19:29:28.25 I2C::Gateway::Port::Error in core_reader -- No Response to BREAK @19:29:28.26 Retry #1 of 3 @19:29:35.09 I2C::Gateway::Port::Error in core_reader -- No Response to BREAK @19:29:35.11 Retry #2 of 3 @19:29:41.93 I2C::Gateway::Port::Error in core_reader -- No Response to BREAK @19:29:41.94 Retry #3 of 3 @19:29:48.77 Try 'Gate.reconnect' to recover @19:35:05.25 -> Gate.reconnect @19:35:12.08 I2C::Gateway::Port::Error in core_reader -- No Response to BREAK @19:35:12.09 Retry #1 of 3 @19:35:18.92 I2C::Gateway::Port::Error in core_reader -- No Response to BREAK @19:35:18.93 Retry #2 of 3 @19:35:25.75 I2C::Gateway::Port::Error in core_reader -- No Response to BREAK @19:35:25.76 Retry #3 of 3 @19:35:32.58 Try 'Gate.reconnect' to recover @21:08:27.74 Session Ended @13:20:06.05UTC17-Mar-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @13:20:08.62 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @13:20:12.58 Polling Can every 10:00 @13:20:12.69 Cleanly exited at 13:17:37UTC17-Mar-23 @13:20:12.73 Loading /home/esp/esp2/mission/DA_WCR_031723.rb mission @13:20:12.78 GoodNews.email "Starting DA_WCR_031723 w/pucks in tube 3\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:20:12, 17.9C, 50% humidity, 15.5psia, 14.002V, 0.202A, 0.202A avg, 2.83W",Subject: "DA_WCR_031723 begun" @13:20:12.80
Preparing to sleep until + 00:00:05 @13:20:12.85 Asleep til + 00:00:05 @13:20:12.87 Continuing at 13:20 @13:20:13.16 Gate.power :core,:OFF @13:20:13.17 Gate.power :raw,:OFF @13:20:13.18 Shutdown @13:20:15.94 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_031723.rb:14\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:20:12, 17.9C, 50% humidity, 15.5psia, 14.002V, 0.202A, 0.202A avg, 2.83W",Subject: "Asleep until 13:20" @13:20:17.31 upsync @13:20:17.77
Powering core bus @13:20:20.21 upsync @13:20:22.92
Gate.power :raw,:ON @13:20:23.74 CS.configure :default @13:20:23.82 PS.configure :default @13:20:23.91 SS.configure :default @13:20:24.00 CC.configure :default @13:20:24.08 PC.configure :default @13:20:24.19 Forearm.configure :loose @13:20:24.27 Elbow.configure :loose @13:20:24.38 SC.configure :loose @13:20:24.47 SE.configure :default @13:20:24.51 CH.coast @13:20:24.56 CH.configure :default @13:20:24.59 PH.coast @13:20:24.64 PH.configure :default @13:20:24.68 All dwarf controllers running firmware version 3.85 @13:20:24.78 Valve.reset @13:20:24.96 Intake.select :close @13:20:25.00 Exhaust.select :close @13:20:25.07 CTV.dial :air @13:20:25.15 PSV.dial :air @13:20:25.25
Forearm.reconfigure :loose @13:20:25.31 Forearm.home.seek :retract @13:20:25.33 PS.reconfigure :default @13:20:25.41 PS.home.seek 0ml @13:20:25.81 CSV.dial :waste1 @13:20:25.89 SSV.dial :spit,avoiding: :exhaust @13:20:26.09 Hand.close @13:20:26.11
Elbow.jog 1500 @13:20:26.68 Elbow.reconfigure :loose @13:20:26.72 Elbow.home.seek :clear @13:20:27.26 CS.reconfigure :fast @13:20:27.29 SS.reconfigure :default @13:20:27.37 CS.home.seek 0ml @13:20:27.41 SS.home.seek 0ml @13:20:31.19
SE.reconfigure :default @13:20:31.23 SE.home.seek :down @13:20:31.86 SC.reconfigure :tight @13:20:31.91 SC.home.seek :initial @13:20:34.07 PS.stop @13:21:01.95 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @13:21:01.96
Retry #1 of 3 @13:21:02.02 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @13:21:06.39 SC.configure :limp @13:21:06.44 Forearm.seek :retract @13:21:06.49 Elbow.seek :hammer @13:21:07.58 Forearm.reconfigure :tight @13:21:07.62 Forearm.seek retracted @13:21:07.94 PC.reconfigure :default @13:21:07.96
CC.reconfigure :default @13:21:08.03 PC.home.seek :open @13:21:08.08
CC.home.seek :open @13:21:11.28 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:21:11.29 Notice.email "At 13:21:11 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_031723.rb:14\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:21:11, 18.0C, 51% humidity, 15.5psia, 13.841V, 0.477A, 0.477A avg, 6.61W",Subject: "Started DA_WCR_031723 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:21:11.31
Starting initialPurge @13:21:11.39 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:21:11.46 Hand.open @13:21:12.36 Elbow.reconfigure :tight @13:21:12.42 Elbow.seek FlushPuck::Garage @13:21:14.25 Elbow.reconfigure :loose @13:21:14.35 Forearm.reconfigure :loose @13:21:14.40 Forearm.seek :garage @13:21:15.19 upsync @13:21:15.53
Hand.close @13:21:16.34 Forearm.seek :retract @13:21:17.42 Elbow.reconfigure :tight @13:21:17.46 Elbow.seek CC @13:21:20.33 Elbow.reconfigure :loose @13:21:20.42 Forearm.reconfigure :tight @13:21:20.46 Forearm.seek CC @13:21:21.69 CC.seek :closed @13:21:25.94 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.76 @13:21:26.01 Hand.open @13:21:26.78 Forearm.reconfigure :loose @13:21:26.83 Forearm.seek :retract @13:21:27.79 Flushing the Collection Valve::Manifold @13:21:27.91 CSV.dial CSR @13:21:29.24 Pulling 5ml of CSR.flush CSR.select :flush @13:21:29.35 CS.reconfigure :default @13:21:29.39 CS.seek 5ml @13:21:42.79 delay 5 seconds @13:21:47.81 CSR.select :air @13:21:47.95 CS.reconfigure :fast @13:21:48.00 CS.seek 7.5ml @13:21:54.54 CSV.dial :waste @13:21:55.81 CS.reconfigure :default @13:21:55.85 CS.seek 0ml @13:22:15.64 Purging external Intake @13:22:15.75 CSV.dial :flush @13:22:16.20 CS.reconfigure :fast @13:22:16.25 CS.seek 10ml @13:22:39.32 delay 5 seconds @13:22:44.34 CSV.dial CBV @13:22:44.37
CBV.dial CSV @13:22:45.43 CTV.dial :intake @13:22:46.18 Intake.select :open @13:22:46.29 CS.seek 0ml @13:23:08.88 Intake.select :close @13:23:08.98 CTV.dial :air @13:23:09.73 Flushing upper manifold @13:23:09.84 CSV.dial :flush @13:23:10.58 CS.seek 5ml @13:23:22.41 delay 5 seconds @13:23:27.43 CSV.dial :air @13:23:27.92 CS.seek 6.999ml @13:23:33.26 CSV.dial CBV @13:23:33.29
CBV.dial CSV @13:23:34.29 CTV.dial :air @13:23:34.31 CS.seek 0ml @13:23:50.51 delay 7 seconds @13:23:57.53 CS.seek 3ml @13:24:04.97 CSV.dial :waste @13:24:05.42 CS.seek 0ml @13:24:13.01 CSV.dial :flush @13:24:13.45 CS.seek 3ml @13:24:20.85 delay 5 seconds @13:24:25.87 CSV.dial :air @13:24:26.36 CS.seek 5ml @13:24:31.63 CSV.dial :air @13:24:31.65 CS.seek 0ml @13:24:43.35 delay 7 seconds @13:24:50.38 CS.seek 3ml @13:24:57.88 CSV.dial :waste @13:24:58.61 CS.seek 0ml @13:25:06.01 Garaging FlushPuck in Collection Clamp @13:25:06.07 Elbow.reconfigure :tight @13:25:06.12 Elbow.seek CC @13:25:06.22 Elbow.reconfigure :loose @13:25:06.31 Forearm.seek CC @13:25:07.29 Hand.close @13:25:07.97 CC.seek :open @13:25:12.30 Forearm.seek :retract @13:25:13.30 Elbow.reconfigure :tight @13:25:13.34 Elbow.seek :garage @13:25:16.27 Elbow.reconfigure :loose @13:25:16.36 Forearm.reconfigure :tight @13:25:16.40 Forearm.seek :garage @13:25:17.54 Hand.open @13:25:18.64 Forearm.reconfigure :loose @13:25:18.75 Forearm.seek :retract @13:25:19.81 da sampling at most 1000ml, wcr at most 1000ml @13:25:19.84 Loading sh1 puck from tube 3 into Collection Clamp @13:25:19.86 DAfans.email "Started da in tube 3 at 13:25:19UTC17-Mar-23\n{1 => 33,\n 2 => 23,\n 3 => 10,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:21:11, 18.0C, 51% humidity, 15.5psia, 13.841V, 0.477A, 0.477A avg, 6.61W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:25:20.11
SC.reconfigure :tight @13:25:20.16 SC.seek 3 @13:25:24.09 upsync @13:25:30.00
SC.configure :limp @13:25:30.20 Elbow.reconfigure :tight @13:25:30.25 Elbow.seek 3 @13:25:32.23 Forearm.reconfigure :tight @13:25:32.27 Forearm.seek 3 + 700 counts @13:25:33.28 SE.reconfigure :pick @13:25:33.33 SE.seek :beamBreak @13:25:35.79 SE.configure :default @13:25:35.94 Elbow.seek 3 @13:25:36.11 Forearm.seek 3 + 300 counts @13:25:36.31 Forearm.reconfigure :loose @13:25:36.35 Hand.close @13:25:36.98 SE.seek :down @13:25:37.16
Forearm.seek :retract @13:25:38.14 Elbow.seek CC @13:25:39.40 SE.stop @13:25:39.74
Elbow.reconfigure :loose @13:25:39.83 Forearm.reconfigure :tight @13:25:39.87 Forearm.seek CC @13:25:41.18 CC.seek :closed @13:25:45.41 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.68 @13:25:45.42 Hand.open @13:25:46.16 Forearm.reconfigure :loose @13:25:46.20 Forearm.seek :retract @13:25:47.22 collectSample 1000ml w/bubble point of 24psi @13:25:47.23 Priming 7ml @13:25:47.24 Intake.select :close @13:25:47.25 Exhaust.select :close @13:25:47.26 SS.hold @13:25:47.45 Calibrating pressure and temperature sensors @13:25:47.48 CTV.dial :air @13:25:47.49 SSV.dial :spit,avoiding: :exhaust @13:25:47.51 SS.reconfigure :fast @13:25:47.55 SS.seek 5ml @13:25:50.52 SSV.dial CBV @13:25:50.54
CBV.dial SSV @13:25:51.88 delay 3.8 seconds @13:25:55.79 SSV.dial :spit @13:25:57.11 SS.seek 0ml @13:26:00.06 SSV.dial CBV @13:26:01.34 CTV.dial :intake @13:26:02.07 SSV.dial CBV @13:26:02.09
CBV.dial SSV @13:26:02.11 Allowing 40s for 7.0ml of remaining 7.0ml @13:26:02.12 Intake.select :open @13:26:04.27 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:26:04.29 SS.reconfigure :inhaling 2.14ml/s @13:26:04.33 SS.seek 7ml @13:26:10.31 Stroke completed -- waiting for pressure to equalize ... @13:26:10.32 SS.reconfigure :exhale @13:26:10.36 SS.hold @13:26:13.27 Intake.select :close @13:26:13.41 SSV.dial :exhaust @13:26:14.16 Exhaust.select :open @13:26:14.26 SS.seek 0ml @13:26:18.65 Exhaust.select :close @13:26:18.76 Sampling 1000.0ml @13:26:18.77 Intake.select :close @13:26:18.78 Exhaust.select :close @13:26:18.79 SS.reconfigure :default @13:26:18.82 SS.hold @13:26:19.76 CTV.dial :intake @13:26:19.77 SSV.dial CBV @13:26:19.81
CBV.dial SSV @13:26:20.53 Allowing 130s for 25.0ml of remaining 1000.0ml @13:26:20.54 Intake.select :open @13:26:22.69 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:26:22.71 SS.reconfigure :inhaling 2.14ml/s @13:26:22.75 SS.seek 25ml @13:26:26.87 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @13:26:26.91 SS.hold @13:26:28.61 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:26:28.63 SS.reconfigure :inhaling 1.93ml/s @13:26:28.67 SS.seek 25ml @13:26:30.93 Vacuum too high -- waiting up to 40 seconds @13:26:30.94 SS.reconfigure :exhale @13:26:30.98 SS.hold @13:26:32.19 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:26:32.21 SS.reconfigure :inhaling 1.74ml/s @13:26:32.25 SS.seek 25ml @13:26:35.21 Vacuum too high -- waiting up to 40 seconds @13:26:35.22 SS.reconfigure :exhale @13:26:35.26 SS.hold @13:26:36.10 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:26:36.12 SS.reconfigure :inhaling 1.57ml/s @13:26:36.16 SS.seek 25ml @13:26:38.24 Vacuum too high -- waiting up to 40 seconds @13:26:38.25 SS.reconfigure :exhale @13:26:38.29 SS.hold @13:26:39.09 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:26:39.11 SS.reconfigure :inhaling 1.41ml/s @13:26:39.15 SS.seek 25ml @13:26:42.30 Vacuum too high -- waiting up to 40 seconds @13:26:42.31 SS.reconfigure :exhale @13:26:42.35 SS.hold @13:26:43.12 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:26:43.13 SS.reconfigure :inhaling 1.27ml/s @13:26:43.17 SS.seek 25ml @13:26:45.87 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @13:26:45.91 SS.hold @13:26:46.62 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:26:46.64 SS.reconfigure :inhaling 1.14ml/s @13:26:46.68 SS.seek 25ml @13:26:49.37 Vacuum too high -- waiting up to 40 seconds SS.reconfigure :exhale @13:26:49.42 SS.hold @13:26:50.07 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:26:50.09 SS.reconfigure :inhaling 1.03ml/s @13:26:50.13 SS.seek 25ml @13:26:54.59 Stroke completed -- waiting for pressure to equalize ... @13:26:54.60 SS.reconfigure :exhale @13:26:54.64 SS.hold @13:26:56.18 Intake.select :close @13:26:56.32 SSV.dial :exhaust @13:26:57.05 Exhaust.select :open @13:26:57.15 SS.seek 0ml @13:27:06.04 Exhaust.select :close @13:27:06.15 SSV.dial CBV @13:27:06.17
CBV.dial SSV @13:27:06.92 Allowing 130s for 25.0ml of remaining 975.0ml @13:27:06.93 Intake.select :open @13:27:09.08 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:27:09.10 SS.reconfigure :inhaling 1.03ml/s @13:27:09.14 SS.seek 25ml @13:27:36.09 Stroke completed -- waiting for pressure to equalize ... @13:27:36.10 SS.reconfigure :exhale @13:27:36.14 SS.hold @13:27:37.32 Intake.select :close @13:27:37.46 SSV.dial :exhaust @13:27:38.20 Exhaust.select :open @13:27:38.31 SS.seek 0ml @13:27:47.50 Exhaust.select :close @13:27:47.60 SSV.dial CBV @13:27:47.62
CBV.dial SSV @13:27:48.37 Allowing 130s for 25.0ml of remaining 950.0ml @13:27:48.38 Intake.select :open @13:27:50.53 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:27:50.55 SS.reconfigure :inhaling 1.03ml/s @13:27:50.59 SS.seek 25ml @13:28:16.51 Stroke completed -- waiting for pressure to equalize ... @13:28:16.52 SS.reconfigure :exhale @13:28:16.56 SS.hold @13:28:17.41 Intake.select :close @13:28:17.55 SSV.dial :exhaust @13:28:18.28 Exhaust.select :open @13:28:18.38 SS.seek 0ml @13:28:27.24 Exhaust.select :close @13:28:27.35 SSV.dial CBV @13:28:27.37
CBV.dial SSV @13:28:28.12 Allowing 130s for 25.0ml of remaining 925.0ml @13:28:28.13 Intake.select :open @13:28:30.28 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:28:30.30 SS.reconfigure :inhaling 1.03ml/s @13:28:30.34 SS.seek 25ml @13:28:57.10 Stroke completed -- waiting for pressure to equalize ... @13:28:57.11 SS.reconfigure :exhale @13:28:57.15 SS.hold @13:28:58.33 Intake.select :close @13:28:58.47 SSV.dial :exhaust @13:28:59.21 Exhaust.select :open @13:28:59.32 SS.seek 0ml @13:29:08.15 Exhaust.select :close @13:29:08.25 SSV.dial CBV @13:29:08.30
CBV.dial SSV @13:29:09.01 Allowing 130s for 25.0ml of remaining 900.0ml @13:29:09.02 Intake.select :open @13:29:11.17 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:29:11.19 SS.reconfigure :inhaling 1.03ml/s @13:29:11.23 SS.seek 25ml @13:29:37.98 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @13:29:38.02 SS.hold @13:29:38.88 Intake.select :close @13:29:39.02 SSV.dial :exhaust @13:29:39.74 Exhaust.select :open @13:29:39.85 SS.seek 0ml @13:29:48.65 Exhaust.select :close @13:29:48.75 SSV.dial CBV @13:29:48.79
CBV.dial SSV @13:29:49.53 Allowing 130s for 25.0ml of remaining 875.0ml @13:29:49.54 Intake.select :open @13:29:51.69 Intake @14.1psia, vacuum from 8.6 to 11.6psi @13:29:51.70 SS.reconfigure :inhaling 1.03ml/s @13:29:51.74 SS.seek 25ml @13:30:18.47 Stroke completed -- waiting for pressure to equalize ... @13:30:18.48 SS.reconfigure :exhale @13:30:18.52 SS.hold @13:30:19.38 Intake.select :close @13:30:19.52 SSV.dial :exhaust @13:30:20.72 Exhaust.select :open @13:30:20.84 SS.seek 0ml @13:30:30.12 Exhaust.select :close @13:30:30.22 SSV.dial CBV @13:30:30.24
CBV.dial SSV @13:30:30.99 Allowing 130s for 25.0ml of remaining 850.0ml @13:30:31.00 Intake.select :open @13:30:33.15 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:30:33.17 SS.reconfigure :inhaling 1.03ml/s @13:30:33.21 SS.seek 25ml @13:30:59.16 Stroke completed -- waiting for pressure to equalize ... @13:30:59.17 SS.reconfigure :exhale @13:30:59.21 SS.hold @13:31:00.16 Intake.select :close @13:31:00.29 SSV.dial :exhaust @13:31:01.04 Exhaust.select :open @13:31:01.14 SS.seek 0ml @13:31:10.32 Exhaust.select :close @13:31:10.42 SSV.dial CBV @13:31:10.45
CBV.dial SSV @13:31:11.19 Allowing 130s for 25.0ml of remaining 825.0ml @13:31:11.20 Intake.select :open @13:31:13.36 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:31:13.37 SS.reconfigure :inhaling 1.03ml/s @13:31:13.41 SS.seek 25ml @13:31:35.10 Vacuum too high -- waiting up to 40 seconds @13:31:35.11 SS.reconfigure :exhale @13:31:35.16 SS.hold @13:31:35.54 Intake @9.2psia, vacuum from 3.7 to 6.7psi @13:31:35.56 SS.reconfigure :inhaling 0.93ml/s @13:31:35.60 SS.seek 25ml @13:31:40.49 Stroke completed -- waiting for pressure to equalize ... @13:31:40.50 SS.reconfigure :exhale @13:31:40.54 SS.hold @13:31:41.19 Intake.select :close @13:31:41.33 SSV.dial :exhaust @13:31:42.05 Exhaust.select :open @13:31:42.16 SS.seek 0ml @13:31:51.00 Exhaust.select :close @13:31:51.11 SSV.dial CBV @13:31:51.13
CBV.dial SSV @13:31:51.89 Allowing 130s for 25.0ml of remaining 800.0ml @13:31:51.90 Intake.select :open @13:31:54.05 Intake @13.9psia, vacuum from 8.4 to 11.4psi @13:31:54.06 SS.reconfigure :inhaling 0.93ml/s @13:31:54.11 SS.seek 25ml @13:32:23.41 Stroke completed -- waiting for pressure to equalize ... @13:32:23.42 SS.reconfigure :exhale @13:32:23.46 SS.hold @13:32:24.32 Intake.select :close @13:32:24.45 SSV.dial :exhaust @13:32:25.19 Exhaust.select :open @13:32:25.30 SS.seek 0ml @13:32:34.11 Exhaust.select :close @13:32:34.21 SSV.dial CBV @13:32:34.24
CBV.dial SSV @13:32:34.99 Allowing 130s for 25.0ml of remaining 775.0ml @13:32:35.00 Intake.select :open @13:32:37.15 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:32:37.17 SS.reconfigure :inhaling 0.93ml/s @13:32:37.21 SS.seek 25ml @13:33:06.47 Stroke completed -- waiting for pressure to equalize ... @13:33:06.48 SS.reconfigure :exhale @13:33:06.52 SS.hold @13:33:07.70 Intake.select :close @13:33:07.84 SSV.dial :exhaust @13:33:08.56 Exhaust.select :open @13:33:08.67 SS.seek 0ml @13:33:17.48 Exhaust.select :close @13:33:17.59 SSV.dial CBV @13:33:17.61
CBV.dial SSV @13:33:18.36 Allowing 130s for 25.0ml of remaining 750.0ml @13:33:18.37 Intake.select :open @13:33:20.52 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:33:20.54 SS.reconfigure :inhaling 0.93ml/s @13:33:20.58 SS.seek 25ml @13:33:49.87 Stroke completed -- waiting for pressure to equalize ... @13:33:49.88 SS.reconfigure :exhale @13:33:49.92 SS.hold @13:33:51.57 Intake.select :close @13:33:51.71 SSV.dial :exhaust @13:33:52.45 Exhaust.select :open @13:33:52.56 SS.seek 0ml @13:34:01.81 Exhaust.select :close @13:34:01.91 SSV.dial CBV @13:34:01.94
CBV.dial SSV @13:34:02.69 Allowing 130s for 25.0ml of remaining 725.0ml @13:34:02.70 Intake.select :open @13:34:04.85 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:34:04.86 SS.reconfigure :inhaling 0.93ml/s @13:34:04.91 SS.seek 25ml @13:34:33.33 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @13:34:33.37 SS.hold @13:34:34.91 Intake.select :close @13:34:35.05 SSV.dial :exhaust @13:34:35.78 Exhaust.select :open @13:34:35.89 SS.seek 0ml @13:34:45.00 Exhaust.select :close @13:34:45.10 SSV.dial CBV @13:34:45.13
CBV.dial SSV @13:34:45.86 Allowing 130s for 25.0ml of remaining 700.0ml @13:34:45.87 Intake.select :open @13:34:48.02 Intake @13.9psia, vacuum from 8.4 to 11.4psi @13:34:48.04 SS.reconfigure :inhaling 0.93ml/s @13:34:48.08 SS.seek 25ml @13:35:16.57 Stroke completed -- waiting for pressure to equalize ... @13:35:16.58 SS.reconfigure :exhale @13:35:16.62 SS.hold @13:35:18.16 Intake.select :close @13:35:18.30 SSV.dial :exhaust @13:35:19.05 Exhaust.select :open @13:35:19.15 SS.seek 0ml @13:35:28.57 Exhaust.select :close @13:35:28.68 SSV.dial CBV @13:35:28.70
CBV.dial SSV @13:35:29.44 Allowing 130s for 25.0ml of remaining 675.0ml @13:35:29.45 Intake.select :open @13:35:31.60 Intake @13.9psia, vacuum from 8.4 to 11.4psi @13:35:31.61 SS.reconfigure :inhaling 0.93ml/s @13:35:31.65 SS.seek 25ml @13:35:53.72 Vacuum too high -- waiting up to 40 seconds @13:35:53.73 SS.reconfigure :exhale @13:35:53.77 SS.hold @13:35:54.16 Intake @10.5psia, vacuum from 5.0 to 8.0psi @13:35:54.18 SS.reconfigure :inhaling 0.84ml/s @13:35:54.22 SS.seek 25ml @13:36:01.85 Stroke completed -- waiting for pressure to equalize ... @13:36:01.86 SS.reconfigure :exhale @13:36:02.29 SS.hold @13:36:03.03 Intake.select :close @13:36:03.17 SSV.dial :exhaust @13:36:03.90 Exhaust.select :open @13:36:04.01 SS.seek 0ml @13:36:13.03 Exhaust.select :close @13:36:13.13 SSV.dial CBV @13:36:13.16
CBV.dial SSV @13:36:13.91 Allowing 130s for 25.0ml of remaining 650.0ml @13:36:13.92 Intake.select :open @13:36:16.07 Intake @13.7psia, vacuum from 8.2 to 11.2psi @13:36:16.08 SS.reconfigure :inhaling 0.84ml/s @13:36:16.13 SS.seek 25ml @13:36:48.10 Stroke completed -- waiting for pressure to equalize ... @13:36:48.11 SS.reconfigure :exhale @13:36:48.15 SS.hold @13:36:50.13 Intake.select :close @13:36:50.27 SSV.dial :exhaust @13:36:51.01 Exhaust.select :open @13:36:51.12 SS.seek 0ml @13:36:59.98 Exhaust.select :close @13:37:00.08 SSV.dial CBV @13:37:00.13
CBV.dial SSV @13:37:00.84 Allowing 130s for 25.0ml of remaining 625.0ml @13:37:00.85 Intake.select :open @13:37:03.00 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:37:03.02 SS.reconfigure :inhaling 0.84ml/s @13:37:03.06 SS.seek 25ml @13:37:15.37 Vacuum too high -- waiting up to 40 seconds @13:37:15.38 SS.reconfigure :exhale @13:37:15.43 SS.hold @13:37:16.38 Intake @12.5psia, vacuum from 7.0 to 10.0psi @13:37:16.39 SS.reconfigure :inhaling 0.76ml/s @13:37:16.43 SS.seek 25ml @13:37:33.00 Vacuum too high -- waiting up to 40 seconds @13:37:33.01 SS.reconfigure :exhale @13:37:33.05 SS.hold @13:37:34.68 Intake @12.3psia, vacuum from 6.8 to 9.8psi @13:37:34.70 SS.reconfigure :inhaling 0.69ml/s @13:37:34.74 SS.seek 25ml @13:37:41.40 Stroke completed -- waiting for pressure to equalize ... @13:37:41.41 SS.reconfigure :exhale @13:37:41.45 SS.hold @13:37:42.63 Intake.select :close @13:37:42.77 SSV.dial :exhaust @13:37:43.49 Exhaust.select :open @13:37:43.60 SS.seek 0ml @13:37:52.79 Exhaust.select :close @13:37:52.89 SSV.dial CBV @13:37:52.94
CBV.dial SSV @13:37:53.63 Allowing 130s for 25.0ml of remaining 600.0ml @13:37:53.64 Intake.select :open @13:37:55.80 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:37:55.81 SS.reconfigure :inhaling 0.69ml/s @13:37:55.85 SS.seek 25ml @13:38:33.74 Stroke completed -- waiting for pressure to equalize ... @13:38:33.75 SS.reconfigure :exhale @13:38:33.79 SS.hold @13:38:36.18 Intake.select :close @13:38:36.32 SSV.dial :exhaust @13:38:37.05 Exhaust.select :open @13:38:37.16 SS.seek 0ml @13:38:46.16 Exhaust.select :close @13:38:46.26 SSV.dial CBV @13:38:46.29
CBV.dial SSV @13:38:47.04 Allowing 130s for 25.0ml of remaining 575.0ml @13:38:47.05 Intake.select :open @13:38:49.20 Intake @14.1psia, vacuum from 8.6 to 11.6psi @13:38:49.22 SS.reconfigure :inhaling 0.69ml/s @13:38:49.26 SS.seek 25ml @13:39:28.07 Stroke completed -- waiting for pressure to equalize ... @13:39:28.08 SS.reconfigure :exhale @13:39:28.12 SS.hold @13:39:31.00 Intake.select :close @13:39:31.13 SSV.dial :exhaust @13:39:31.88 Exhaust.select :open @13:39:31.99 SS.seek 0ml @13:39:41.28 Exhaust.select :close @13:39:41.39 SSV.dial CBV @13:39:41.41
CBV.dial SSV @13:39:42.16 Allowing 130s for 25.0ml of remaining 550.0ml @13:39:42.18 Intake.select :open @13:39:44.33 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:39:44.34 SS.reconfigure :inhaling 0.69ml/s @13:39:44.38 SS.seek 25ml @13:40:11.59 Vacuum too high -- waiting up to 40 seconds @13:40:11.60 SS.reconfigure :exhale @13:40:11.69 SS.hold @13:40:13.16 Intake @13.4psia, vacuum from 7.9 to 10.9psi @13:40:13.18 SS.reconfigure :inhaling 0.61ml/s @13:40:13.23 SS.seek 25ml @13:40:25.55 Stroke completed -- waiting for pressure to equalize ... @13:40:25.56 SS.reconfigure :exhale @13:40:25.60 SS.hold @13:40:28.08 Intake.select :close @13:40:28.22 SSV.dial :exhaust @13:40:28.94 Exhaust.select :open @13:40:29.05 SS.seek 0ml @13:40:38.49 Exhaust.select :close @13:40:38.59 SSV.dial CBV @13:40:38.64
CBV.dial SSV @13:40:39.33 Allowing 130s for 25.0ml of remaining 525.0ml @13:40:39.34 Intake.select :open @13:40:41.49 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:40:41.51 SS.reconfigure :inhaling 0.61ml/s @13:40:41.55 SS.seek 25ml @13:41:23.77 Stroke completed -- waiting for pressure to equalize ... @13:41:23.78 SS.reconfigure :exhale @13:41:23.82 SS.hold @13:41:26.71 Intake.select :close @13:41:26.85 SSV.dial :exhaust @13:41:27.60 Exhaust.select :open @13:41:27.70 SS.seek 0ml @13:41:36.63 Exhaust.select :close @13:41:36.73 SSV.dial CBV @13:41:36.76
CBV.dial SSV @13:41:37.50 Allowing 130s for 25.0ml of remaining 500.0ml @13:41:37.51 Intake.select :open @13:41:39.66 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:41:39.68 SS.reconfigure :inhaling 0.61ml/s @13:41:39.72 SS.seek 25ml @13:42:22.76 Stroke completed -- waiting for pressure to equalize ... @13:42:22.77 SS.reconfigure :exhale @13:42:22.82 SS.hold @13:42:26.34 Intake.select :close @13:42:26.48 SSV.dial :exhaust @13:42:27.22 Exhaust.select :open @13:42:27.32 SS.seek 0ml @13:42:36.78 Exhaust.select :close @13:42:36.88 SSV.dial CBV @13:42:36.92
CBV.dial SSV @13:42:37.66 Allowing 130s for 25.0ml of remaining 475.0ml @13:42:37.67 Intake.select :open @13:42:39.82 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:42:39.83 SS.reconfigure :inhaling 0.61ml/s @13:42:39.88 SS.seek 25ml @13:43:13.89 Vacuum too high -- waiting up to 40 seconds @13:43:13.90 SS.reconfigure :exhale @13:43:13.95 SS.hold @13:43:16.44 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:43:16.45 SS.reconfigure :inhaling 0.56ml/s @13:43:16.49 SS.seek 25ml @13:43:25.92 Stroke completed -- waiting for pressure to equalize ... @13:43:25.93 SS.reconfigure :exhale @13:43:25.97 SS.hold @13:43:28.85 Intake.select :close @13:43:28.99 SSV.dial :exhaust @13:43:29.72 Exhaust.select :open @13:43:29.82 SS.seek 0ml @13:43:39.26 Exhaust.select :close @13:43:39.36 SSV.dial CBV @13:43:39.39
CBV.dial SSV @13:43:40.12 Allowing 130s for 25.0ml of remaining 450.0ml @13:43:40.13 Intake.select :open @13:43:42.28 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:43:42.30 SS.reconfigure :inhaling 0.56ml/s @13:43:42.35 SS.seek 25ml @13:44:28.82 Stroke completed -- waiting for pressure to equalize ... @13:44:28.83 SS.reconfigure :exhale @13:44:28.87 SS.hold @13:44:32.75 Intake.select :close @13:44:32.89 SSV.dial :exhaust @13:44:33.64 Exhaust.select :open @13:44:33.75 SS.seek 0ml @13:44:43.17 Exhaust.select :close @13:44:43.27 SSV.dial CBV @13:44:43.29
CBV.dial SSV @13:44:44.03 Allowing 130s for 25.0ml of remaining 425.0ml @13:44:44.04 Intake.select :open @13:44:46.19 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:44:46.21 SS.reconfigure :inhaling 0.56ml/s @13:44:46.25 SS.seek 25ml @13:45:32.71 Stroke completed -- waiting for pressure to equalize ... @13:45:32.72 SS.reconfigure :exhale @13:45:32.76 SS.hold @13:45:38.30 Intake.select :close @13:45:38.44 SSV.dial :exhaust @13:45:39.18 Exhaust.select :open @13:45:39.29 SS.seek 0ml @13:45:48.84 Exhaust.select :close @13:45:48.94 SSV.dial CBV @13:45:48.97
CBV.dial SSV @13:45:49.70 Allowing 130s for 25.0ml of remaining 400.0ml @13:45:49.71 Intake.select :open @13:45:51.86 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:45:51.87 SS.reconfigure :inhaling 0.56ml/s @13:45:51.92 SS.seek 25ml @13:46:20.83 Vacuum too high -- waiting up to 40 seconds @13:46:20.84 SS.reconfigure :exhale @13:46:20.88 SS.hold @13:46:22.84 Intake @12.9psia, vacuum from 7.4 to 10.4psi @13:46:22.85 SS.reconfigure :inhaling 0.50ml/s @13:46:22.89 SS.seek 25ml @13:46:43.11 Stroke completed -- waiting for pressure to equalize ... @13:46:43.12 SS.reconfigure :exhale @13:46:43.17 SS.hold @13:46:47.91 Intake.select :close @13:46:48.05 SSV.dial :exhaust @13:46:48.79 Exhaust.select :open @13:46:48.90 SS.seek 0ml @13:46:57.77 Exhaust.select :close @13:46:57.87 SSV.dial CBV @13:46:57.91
CBV.dial SSV @13:46:58.63 Allowing 130s for 25.0ml of remaining 375.0ml @13:46:58.64 Intake.select :open @13:47:00.79 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:47:00.81 SS.reconfigure :inhaling 0.50ml/s @13:47:00.85 SS.seek 25ml @13:47:53.49 Stroke completed -- waiting for pressure to equalize ... @13:47:53.50 SS.reconfigure :exhale @13:47:53.54 SS.hold @13:47:59.06 Intake.select :close @13:47:59.20 SSV.dial :exhaust @13:47:59.94 Exhaust.select :open @13:48:00.05 SS.seek 0ml @13:48:09.44 Exhaust.select :close @13:48:09.54 SSV.dial CBV @13:48:09.57
CBV.dial SSV @13:48:10.30 Allowing 130s for 25.0ml of remaining 350.0ml @13:48:10.31 Intake.select :open @13:48:12.46 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:48:12.48 SS.reconfigure :inhaling 0.50ml/s @13:48:12.52 SS.seek 25ml @13:48:59.70 Vacuum too high -- waiting up to 40 seconds @13:48:59.71 SS.reconfigure :exhale @13:48:59.75 SS.hold @13:49:03.08 Intake @13.4psia, vacuum from 7.9 to 10.9psi @13:49:03.09 SS.reconfigure :inhaling 0.46ml/s @13:49:03.13 SS.seek 25ml @13:49:08.77 Stroke completed -- waiting for pressure to equalize ... @13:49:08.78 SS.reconfigure :exhale @13:49:08.82 SS.hold @13:49:12.27 Intake.select :close @13:49:12.41 SSV.dial :exhaust @13:49:13.14 Exhaust.select :open @13:49:13.25 SS.seek 0ml @13:49:22.49 Exhaust.select :close @13:49:22.59 SSV.dial CBV @13:49:22.61
CBV.dial SSV @13:49:23.38 Allowing 130s for 25.0ml of remaining 325.0ml @13:49:23.39 Intake.select :open @13:49:25.54 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:49:25.56 SS.reconfigure :inhaling 0.46ml/s @13:49:25.60 SS.seek 25ml @13:50:22.97 Stroke completed -- waiting for pressure to equalize ... @13:50:22.98 SS.reconfigure :exhale @13:50:23.02 SS.hold @13:50:28.54 Intake.select :close @13:50:28.68 SSV.dial :exhaust @13:50:29.42 Exhaust.select :open @13:50:29.53 SS.seek 0ml @13:50:38.59 Exhaust.select :close @13:50:38.71 SSV.dial CBV @13:50:38.73
CBV.dial SSV @13:50:39.49 Allowing 130s for 25.0ml of remaining 300.0ml @13:50:39.50 Intake.select :open @13:50:41.65 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:50:41.66 SS.reconfigure :inhaling 0.46ml/s @13:50:41.70 SS.seek 25ml @13:51:39.64 Stroke completed -- waiting for pressure to equalize ... @13:51:39.65 SS.reconfigure :exhale @13:51:39.69 SS.hold @13:51:47.04 Intake.select :close @13:51:47.18 SSV.dial :exhaust @13:51:47.90 Exhaust.select :open @13:51:48.01 SS.seek 0ml @13:51:57.42 Exhaust.select :close @13:51:57.52 SSV.dial CBV @13:51:57.55
CBV.dial SSV @13:51:58.28 Allowing 130s for 25.0ml of remaining 275.0ml @13:51:58.29 Intake.select :open @13:52:00.44 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:52:00.46 SS.reconfigure :inhaling 0.46ml/s @13:52:00.50 SS.seek 25ml @13:52:35.47 Vacuum too high -- waiting up to 40 seconds @13:52:35.48 SS.reconfigure :exhale @13:52:35.52 SS.hold @13:52:36.79 Intake @12.7psia, vacuum from 7.2 to 10.2psi @13:52:36.81 SS.reconfigure :inhaling 0.41ml/s @13:52:36.85 SS.seek 25ml @13:53:02.48 Stroke completed -- waiting for pressure to equalize ... @13:53:02.49 SS.reconfigure :exhale @13:53:02.53 SS.hold @13:53:08.92 Intake.select :close @13:53:09.06 SSV.dial :exhaust @13:53:09.81 Exhaust.select :open @13:53:09.93 SS.seek 0ml @13:53:19.34 Exhaust.select :close @13:53:19.44 SSV.dial CBV @13:53:19.46
CBV.dial SSV @13:53:20.20 Allowing 130s for 25.0ml of remaining 250.0ml @13:53:20.21 Intake.select :open @13:53:22.36 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:53:22.82 SS.reconfigure :inhaling 0.41ml/s @13:53:22.87 SS.seek 25ml @13:54:27.52 Stroke completed -- waiting for pressure to equalize ... @13:54:27.53 SS.reconfigure :exhale @13:54:27.57 SS.hold @13:54:35.01 Intake.select :close @13:54:35.15 SSV.dial :exhaust @13:54:35.90 Exhaust.select :open @13:54:36.00 SS.seek 0ml @13:54:44.93 Exhaust.select :close @13:54:45.03 SSV.dial CBV @13:54:45.06
CBV.dial SSV @13:54:45.79 Allowing 130s for 25.0ml of remaining 225.0ml @13:54:45.80 Intake.select :open @13:54:47.95 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:54:47.97 SS.reconfigure :inhaling 0.41ml/s @13:54:48.01 SS.seek 25ml @13:55:55.08 Stroke completed -- waiting for pressure to equalize ... @13:55:55.09 SS.reconfigure :exhale @13:55:55.13 SS.hold @13:56:03.57 Intake.select :close @13:56:03.71 SSV.dial :exhaust @13:56:04.46 Exhaust.select :open @13:56:04.56 SS.seek 0ml @13:56:14.03 Exhaust.select :close @13:56:14.14 SSV.dial CBV @13:56:14.16
CBV.dial SSV @13:56:14.90 Allowing 130s for 25.0ml of remaining 200.0ml @13:56:14.91 Intake.select :open @13:56:17.06 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:56:17.07 SS.reconfigure :inhaling 0.41ml/s @13:56:17.11 SS.seek 25ml @13:57:23.92 Stroke completed -- waiting for pressure to equalize ... @13:57:23.93 SS.reconfigure :exhale @13:57:23.97 SS.hold @13:57:32.50 Intake.select :close @13:57:32.64 SSV.dial :exhaust @13:57:33.38 Exhaust.select :open @13:57:33.48 SS.seek 0ml @13:57:42.55 Exhaust.select :close @13:57:42.65 SSV.dial CBV @13:57:42.68
CBV.dial SSV @13:57:43.41 Allowing 130s for 25.0ml of remaining 175.0ml @13:57:43.42 Intake.select :open @13:57:45.57 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:57:45.59 SS.reconfigure :inhaling 0.41ml/s @13:57:45.63 SS.seek 25ml @13:58:52.33 Vacuum too high -- waiting up to 40 seconds @13:58:52.34 SS.reconfigure :exhale @13:58:52.38 SS.hold @13:58:56.54 Intake @13.6psia, vacuum from 8.1 to 11.1psi @13:58:56.56 SS.reconfigure :inhaling 0.37ml/s @13:58:56.60 SS.seek 25ml @13:58:57.42 Stroke completed -- waiting for pressure to equalize ... @13:58:57.43 SS.reconfigure :exhale @13:58:57.48 SS.hold @13:59:04.25 Intake.select :close @13:59:04.39 SSV.dial :exhaust @13:59:05.12 Exhaust.select :open @13:59:05.23 SS.seek 0ml @13:59:14.34 Exhaust.select :close @13:59:14.44 SSV.dial CBV @13:59:14.47
CBV.dial SSV @13:59:15.20 Allowing 130s for 25.0ml of remaining 150.0ml @13:59:15.21 Intake.select :open @13:59:17.36 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:59:17.38 SS.reconfigure :inhaling 0.37ml/s @13:59:17.42 SS.seek 25ml @14:00:35.31 Stroke completed -- waiting for pressure to equalize ... @14:00:35.32 SS.reconfigure :exhale @14:00:35.36 SS.hold @14:00:43.79 Intake.select :close @14:00:43.93 SSV.dial :exhaust @14:00:44.67 Exhaust.select :open @14:00:44.77 SS.seek 0ml @14:00:54.12 Exhaust.select :close @14:00:54.22 SSV.dial CBV @14:00:54.27
CBV.dial SSV @14:00:54.96 Allowing 130s for 25.0ml of remaining 125.0ml @14:00:54.98 Intake.select :open @14:00:57.13 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:00:57.14 SS.reconfigure :inhaling 0.37ml/s @14:00:57.18 SS.seek 25ml @14:02:14.54 Stroke completed -- waiting for pressure to equalize ... @14:02:14.55 SS.reconfigure :exhale @14:02:14.59 SS.hold @14:02:24.24 Intake.select :close @14:02:24.37 SSV.dial :exhaust @14:02:25.12 Exhaust.select :open @14:02:25.23 SS.seek 0ml @14:02:34.71 Exhaust.select :close @14:02:34.81 SSV.dial CBV @14:02:34.86
CBV.dial SSV @14:02:35.57 Allowing 130s for 25.0ml of remaining 100.0ml @14:02:35.58 Intake.select :open @14:02:37.73 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:02:37.75 SS.reconfigure :inhaling 0.37ml/s @14:02:37.79 SS.seek 25ml @14:03:55.97 Stroke completed -- waiting for pressure to equalize ... @14:03:55.98 SS.reconfigure :exhale @14:03:56.03 SS.hold @14:04:08.59 Intake.select :close @14:04:08.73 SSV.dial :exhaust @14:04:09.47 Exhaust.select :open @14:04:09.58 SS.seek 0ml @14:04:18.61 Exhaust.select :close @14:04:18.72 SSV.dial CBV @14:04:18.74
CBV.dial SSV @14:04:19.49 Allowing 130s for 25.0ml of remaining 75.0ml @14:04:19.50 Intake.select :open @14:04:21.66 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:04:21.67 SS.reconfigure :inhaling 0.37ml/s @14:04:21.71 SS.seek 25ml @14:05:30.73 Vacuum too high -- waiting up to 40 seconds @14:05:30.74 SS.reconfigure :exhale @14:05:30.78 SS.hold @14:05:35.99 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:05:36.00 SS.reconfigure :inhaling 0.33ml/s @14:05:36.05 SS.seek 25ml @14:05:46.36 Stroke completed -- waiting for pressure to equalize ... @14:05:46.37 SS.reconfigure :exhale @14:05:46.41 SS.hold @14:05:56.15 Intake.select :close @14:05:56.28 SSV.dial :exhaust @14:05:57.04 Exhaust.select :open @14:05:57.15 SS.seek 0ml @14:06:06.75 Exhaust.select :close @14:06:06.85 SSV.dial CBV @14:06:06.88
CBV.dial SSV @14:06:07.63 Allowing 130s for 25.0ml of remaining 50.0ml @14:06:07.64 Intake.select :open @14:06:09.79 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:06:09.80 SS.reconfigure :inhaling 0.33ml/s @14:06:09.85 SS.seek 25ml @14:07:39.39 Stroke completed -- waiting for pressure to equalize ... @14:07:39.40 SS.reconfigure :exhale @14:07:39.44 SS.hold @14:07:50.44 Intake.select :close @14:07:50.58 SSV.dial :exhaust @14:07:51.31 Exhaust.select :open @14:07:51.42 SS.seek 0ml @14:08:00.87 Exhaust.select :close @14:08:00.97 SSV.dial CBV @14:08:01.00
CBV.dial SSV @14:08:01.75 Allowing 130s for 25.0ml of remaining 25.0ml @14:08:01.76 Intake.select :open @14:08:03.91 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:08:03.92 SS.reconfigure :inhaling 0.33ml/s @14:08:03.97 SS.seek 24.97ml @14:09:31.23 Stroke completed -- waiting for pressure to equalize ... @14:09:31.24 SS.reconfigure :exhale @14:09:31.28 SS.hold @14:09:47.47 Intake.select :close @14:09:47.61 SSV.dial :exhaust @14:09:48.35 Exhaust.select :open @14:09:48.46 SS.seek 0ml @14:09:57.94 Exhaust.select :close @14:09:58.05 Sampled 1000.0ml @14:09:58.06 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:09:58.07 Intake.select :close @14:09:58.12 CSV.dial :waste @14:09:58.19 CS.seek 0ml @14:09:58.26 CSV.dial :air @14:09:58.29 SSV.dial CBV,avoiding: :exhaust @14:09:58.33
CBV.dial SSV @14:09:59.17 CS.seek 10ml @14:10:00.18
CTV.dial :air @14:10:00.92 delay 15 seconds @14:10:15.94 SSV.dial :spit,avoiding: :exhaust @14:10:17.26 CTV.dial CSV,avoiding: :intake @14:10:17.30
SS.seek 0ml @14:10:17.41 SSV.dial CBV,avoiding: :exhaust @14:10:23.07 CBV.dial between SSV and waste2 @14:10:23.45 CTV.dial CSV,avoiding: :air @14:10:23.48
CSV.dial CTV @14:10:24.07 CS.reconfigure :pressurize @14:10:24.11 CS.seek 0ml @14:10:44.56 BubblePoint: Intake @40.5psia, Outlet @16.4psia @14:10:44.57 CBV.dial SSV @14:10:44.89 SS.reconfigure :default @14:10:44.93 SS.seek 10ml @14:10:50.66 delay 15 seconds @14:11:05.78 Evacuated: Intake @18.9psia, Outlet @11.5psia @14:11:05.79 SSV.dial :spit,avoiding: :exhaust @14:11:07.11 SS.reconfigure :exhale @14:11:07.15 SS.seek 0ml @14:11:12.30 SS.reconfigure :fast @14:11:12.34 SS.seek 5ml @14:11:15.18 SSV.dial CBV,avoiding: :exhaust @14:11:15.22
CBV.dial SSV @14:11:16.53 CTV.dial :air,avoiding: :intake @14:11:17.82 CSV.dial CTV @14:11:17.86
SS.seek 10ml @14:11:18.86 CSV.dial :air @14:11:19.30 CS.reconfigure :fast @14:11:19.34 CS.seek 0ml @14:11:20.71
SSV.dial :spit,avoiding: :exhaust @14:11:22.03 SS.reconfigure :exhale @14:11:22.07 SS.seek 0ml @14:11:24.80 CS.stop @14:11:27.45
SS.reconfigure :fast @14:11:27.49 SS.seek 5ml @14:11:30.19 SSV.dial CBV,avoiding: :exhaust @14:11:30.22
CBV.dial SSV @14:11:32.53 SSV.dial :spit,avoiding: :exhaust @14:11:33.84 SS.reconfigure :default @14:11:33.88 SS.seek 0ml @14:11:37.21 SS.stop @14:11:37.24 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:11:37.25 Intake.select :close @14:11:37.32 CSV.dial :waste @14:11:37.43 SSV.dial CBV,avoiding: :exhaust @14:11:37.46
CBV.dial SSV @14:11:38.11 CS.seek 0ml @14:11:38.18 CSV.dial :air @14:11:38.79
CTV.dial :air @14:11:38.80 delay 15 seconds @14:11:38.91 CS.seek 10ml @14:11:53.82
SSV.dial :spit,avoiding: :exhaust @14:11:55.14 CTV.dial CSV,avoiding: :intake @14:11:55.17
SS.reconfigure :exhale @14:11:55.26 SS.seek 0ml @14:11:55.79 SSV.dial CBV,avoiding: :exhaust @14:12:01.99 CBV.dial between SSV and waste2 @14:12:02.38 CTV.dial CSV,avoiding: :air @14:12:02.41
CSV.dial CTV @14:12:02.84 CS.reconfigure :pressurize @14:12:02.89 CS.seek 0ml @14:12:24.09 BubblePoint: Intake @39.9psia, Outlet @15.6psia @14:12:24.10 CBV.dial SSV @14:12:24.42 SS.reconfigure :default @14:12:24.46 SS.seek 10ml @14:12:30.10 delay 15 seconds @14:12:45.21 Evacuated: Intake @19.5psia, Outlet @12.3psia @14:12:45.22 SSV.dial :spit,avoiding: :exhaust @14:12:46.53 SS.reconfigure :exhale @14:12:46.57 SS.seek 0ml @14:12:51.95 SS.reconfigure :fast @14:12:51.99 SS.seek 5ml @14:12:54.84 SSV.dial CBV,avoiding: :exhaust @14:12:54.86
CBV.dial SSV @14:12:56.18 CTV.dial :air,avoiding: :intake @14:12:57.46 CSV.dial CTV @14:12:57.50
SS.seek 10ml @14:12:58.49 CSV.dial :air @14:12:58.93 CS.reconfigure :fast @14:12:58.98 CS.seek 0ml @14:12:59.99
SSV.dial :spit,avoiding: :exhaust @14:13:01.32 SS.reconfigure :exhale @14:13:01.36 SS.seek 0ml @14:13:03.86 CS.stop @14:13:06.79
SS.reconfigure :fast @14:13:06.84 SS.seek 5ml @14:13:09.68 SSV.dial CBV,avoiding: :exhaust @14:13:09.70
CBV.dial SSV @14:13:12.05 SSV.dial :spit,avoiding: :exhaust @14:13:13.35 SS.reconfigure :default @14:13:13.40 SS.seek 0ml @14:13:16.72 SS.stop @14:13:16.76 Lysing: Adding 2.0ml AqMeOH @14:13:16.77 CBV.dial between CSV and waste1,avoiding: :DBV @14:13:18.66 CTV.dial CSV @14:13:19.96 CSV.dial CSR @14:13:20.73 Pulling 1ml of CSR.AqMeOH @14:13:20.74 CSR.select :AqMeOH @14:13:20.85 CS.reconfigure :default @14:13:20.89 CS.seek 1ml @14:13:24.24 delay 5 seconds @14:13:29.26 CSR.select :air @14:13:29.41 CS.seek 5.999ml @14:13:42.93 delay 2 seconds @14:13:44.94 CSV.dial :waste2 @14:13:45.37 CS.reconfigure :fast @14:13:45.41 CS.seek 0ml @14:13:59.43 CSV.dial CSR @14:13:59.92 Pulling 2ml of CSR.AqMeOH @14:13:59.93 CSR.select :AqMeOH @14:14:00.03 CS.reconfigure :default @14:14:00.08 CS.seek 2ml @14:14:05.93 delay 5 seconds @14:14:10.95 CSR.select :air @14:14:11.10 CS.seek 5ml @14:14:19.49 delay 2 seconds @14:14:21.50 CSV.dial CTV @14:14:21.93 CS.seek 1ml @14:14:32.90 Before lysing Collection Puck Heater at 11C/39.6psia @14:14:32.92 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @14:14:32.94 CH.seek 70C,8:00 @14:16:45.84 CH.hold 10:00,70C @14:16:46.00 Begun lysing Collection Puck Heater at 68.1C/42.0psia @14:26:46.01 Ended lysing Collection Puck Heater at 70C/43.0psia @14:26:46.03 CS.seek 0ml @14:26:49.38 CTV.dial between spare and air,avoiding: :intake @14:26:50.38 CSV.dial :air @14:26:50.82 CS.seek 5ml @14:27:04.34 CSV.dial CTV @14:27:04.78 delay 2 seconds @14:27:06.80 CSV.dial :air @14:27:07.24 delay 2 seconds @14:27:09.25 CSV.dial CTV @14:27:09.70 delay 2 seconds @14:27:11.72 CSV.dial :air @14:27:12.16 delay 2 seconds @14:27:14.18 CS.seek 0ml @14:27:28.18 Puck @70.5psia after final lysis pressurization @14:27:28.19 Recovering Lysate into Collection syringe @14:27:28.20 CSV.dial CBV @14:27:29.20 CBV.dial CSV @14:27:29.58 CS.seek 5ml @14:27:42.97 delay 5 seconds @14:27:47.99 CTV.dial :air @14:27:48.50 CS.seek 7ml @14:27:54.37 delay 5 seconds @14:27:59.39 CSV.dial :air2 @14:27:59.83 CS.seek 10ml @14:28:08.22 CSV.dial between air and CTV,avoiding: CBV @14:28:09.35 Lysing Complete @14:28:09.42 Storing sh1 puck in Collection Clamp to tube 2 @14:28:09.57 SC.reconfigure :tight @14:28:09.61 SC.seek 2 @14:28:13.17 SC.configure :limp @14:28:13.37 Elbow.reconfigure :tight @14:28:13.41 Elbow.seek 2 @14:28:15.24 Forearm.reconfigure :tight @14:28:15.28 Forearm.seek 2 + 700 counts @14:28:16.23 SE.reconfigure :pick @14:28:16.28 SE.seek :beamBreak @14:28:17.93 SE.configure :default @14:28:18.07 Elbow.reconfigure :limp @14:28:18.12 Forearm.reconfigure :loose @14:28:18.15 Forearm.seek 2 @14:28:18.58 Elbow.hold @14:28:18.64 Elbow.reconfigure :tight @14:28:18.73 SE.seek 22.34 Pucks below Top Plate @14:28:19.25 Forearm.force -20 @14:28:19.29 Forearm.seek 2 + 286 counts @14:28:19.61 Forearm.reconfigure :tight @14:28:19.65 Forearm.seek 2 + 700 counts @14:28:19.92 SE.seek 23.34 Pucks below Top Plate @14:28:20.57 Forearm.reconfigure :loose @14:28:20.61 Forearm.seek :retract @14:28:21.52 Elbow.seek CC @14:28:23.10 Elbow.reconfigure :loose @14:28:23.19 Forearm.seek CC @14:28:24.19 Hand.close @14:28:24.97 CC.seek :open @14:28:29.40 Forearm.seek :retract @14:28:30.38 Elbow.reconfigure :tight @14:28:30.42 Elbow.seek 2 @14:28:32.19 Elbow.reconfigure :limp @14:28:32.23 Forearm.seek 2 @14:28:33.33 Elbow.hold @14:28:33.39 Elbow.reconfigure :tight @14:28:33.43 Hand.open @14:28:34.34 Forearm.force -20 @14:28:34.37 Forearm.seek 2 + 318 counts @14:28:34.66 Forearm.reconfigure :tight @14:28:34.70 Forearm.seek 2 + 700 counts @14:28:35.05 SE.seek :down @14:28:36.19
Forearm.reconfigure :loose @14:28:36.24 Forearm.seek :retract @14:28:36.26 SE.stop @14:28:37.18
Tranferring DA @14:28:37.19 Beginning samplePassC @14:28:37.20 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:28:37.38 Elbow.seek FlushPuck::Garage @14:28:39.24 Elbow.reconfigure :loose @14:28:39.33 Forearm.seek :garage @14:28:40.41 Hand.close @14:28:41.08 Forearm.seek :retract @14:28:42.21 Elbow.reconfigure :tight @14:28:42.25 Elbow.seek CC @14:28:45.18 Elbow.reconfigure :loose @14:28:45.27 Forearm.reconfigure :tight @14:28:45.31 Forearm.seek CC @14:28:46.98 CC.seek :closed @14:28:51.21 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.87 @14:28:51.28 Hand.open @14:28:52.03 Forearm.reconfigure :loose @14:28:52.07 Forearm.seek :retract @14:28:53.14 PSV.dial PSR @14:28:54.48 Pulling 1.5ml of PSR.flush @14:28:54.49 PSR.select :flush @14:28:54.61 PS.seek 1.5ml @14:29:10.71 delay 5 seconds @14:29:15.73 PSV.dial :waste1 @14:29:16.17 PS.seek 0ml @14:29:32.30 PSV.dial PSR @14:29:32.86 PSR.select :air @14:29:32.98 PS.seek 2.5ml @14:29:59.12 PRV.dial CBV,avoiding: :ab2 @14:29:59.14
CBV.dial PRV @14:30:00.80 PSV.dial :mixloop @14:30:01.94 CTV.dial :air,avoiding: :intake @14:30:01.96 PTV.dial :PRVmixing @14:30:03.20 PS.seek 0ml @14:30:29.46 PSV.dial :air @14:30:29.92 PS.seek 2.5ml @14:30:56.08 PSV.dial :mixloop @14:30:56.53 PS.seek 0ml @14:31:22.64 PSV.dial :air @14:31:23.09 PS.seek 2.5ml @14:31:49.30 PSV.dial :mixloop @14:31:49.74 PS.seek 0ml @14:32:15.92 Garaging FlushPuck in Collection Clamp @14:32:15.99 Elbow.reconfigure :tight @14:32:16.04 Elbow.seek CC @14:32:16.15 Elbow.reconfigure :loose @14:32:16.23 Forearm.seek CC @14:32:17.17 Hand.close @14:32:17.84 CC.seek :open @14:32:22.19 Forearm.seek :retract @14:32:23.22 Elbow.reconfigure :tight @14:32:23.26 Elbow.seek :garage @14:32:26.13 Elbow.reconfigure :loose @14:32:26.21 Forearm.reconfigure :tight @14:32:26.25 Forearm.seek :garage @14:32:27.36 Hand.open @14:32:28.15 Forearm.reconfigure :loose @14:32:28.19 Forearm.seek :retract @14:32:29.21 PSV.dial :mixloop @14:32:29.23 PTV.dial :PRVmixing @14:32:29.24 PRV.dial CBV @14:32:29.26
CBV.dial PRV @14:32:29.28 CTV.dial :air,avoiding: :intake @14:32:29.29 Loading lyfil puck from tube 3 into Collection Clamp @14:32:29.51 SC.reconfigure :tight @14:32:29.55 SC.seek 3 @14:32:33.11 SC.configure :limp @14:32:33.30 Elbow.reconfigure :tight @14:32:33.34 Elbow.seek 3 @14:32:35.29 Forearm.reconfigure :tight @14:32:35.33 Forearm.seek 3 + 700 counts @14:32:36.38 SE.reconfigure :pick @14:32:36.43 SE.seek :beamBreak @14:32:38.95 SE.configure :default @14:32:39.10 Elbow.seek 3 @14:32:39.26 Forearm.seek 3 + 300 counts @14:32:39.46 Forearm.reconfigure :loose @14:32:39.50 Hand.close @14:32:40.10 SE.seek :down @14:32:40.75
Forearm.seek :retract @14:32:41.72 Elbow.seek CC @14:32:42.32 SE.stop @14:32:43.27
Elbow.reconfigure :loose @14:32:43.36 Forearm.reconfigure :tight @14:32:43.40 Forearm.seek CC @14:32:44.70 CC.seek :closed @14:32:48.93 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.66 @14:32:48.94 Hand.open @14:32:49.77 Forearm.reconfigure :loose @14:32:49.81 Forearm.seek :retract @14:32:50.81 CTV.dial CSV,avoiding: :intake @14:32:52.10 CSV.dial CTV @14:32:52.41 PS.seek 2.5ml @14:33:18.64 CS.seek 5.5ml @14:33:30.96 delay 8 seconds @14:33:38.98 PSV.dial :air @14:33:39.42 delay 2 seconds @14:33:41.44 PSV.dial :waste2 @14:33:42.18 PS.seek 0ml @14:34:08.39 CBV.dial between PRV and waste1 @14:34:08.71 CSV.dial :air @14:34:09.17 CS.seek 10ml @14:34:21.36 CSV.dial CTV @14:34:21.81 CS.seek 0ml @14:34:47.83 CBV.dial :waste1 @14:34:48.13 delay 5 seconds @14:34:53.15 CTV.dial :air,avoiding: :intake @14:34:54.50 Storing lyfil puck in Collection Clamp to tube 2 @14:34:54.66 SC.reconfigure :tight @14:34:54.70 SC.seek 2 @14:34:58.27 SC.configure :limp @14:34:58.46 Elbow.reconfigure :tight @14:34:58.50 Elbow.seek 2 @14:35:00.35 Forearm.reconfigure :tight @14:35:00.39 Forearm.seek 2 + 700 counts @14:35:01.41 SE.reconfigure :pick @14:35:01.46 SE.seek :beamBreak @14:35:03.09 SE.configure :default @14:35:03.23 Elbow.reconfigure :limp @14:35:03.28 Forearm.reconfigure :loose @14:35:03.32 Forearm.seek 2 @14:35:03.69 Elbow.hold @14:35:03.75 Elbow.reconfigure :tight @14:35:03.84 SE.seek 23.35 Pucks below Top Plate @14:35:04.32 Forearm.force -20 @14:35:04.35 Forearm.seek 2 + 308 counts @14:35:04.63 Forearm.reconfigure :tight @14:35:04.68 Forearm.seek 2 + 700 counts @14:35:05.00 SE.seek 24.35 Pucks below Top Plate @14:35:05.55 Forearm.reconfigure :loose @14:35:05.59 Forearm.seek :retract @14:35:06.48 Elbow.seek CC @14:35:07.98 Elbow.reconfigure :loose @14:35:08.07 Forearm.seek CC @14:35:09.07 Hand.close @14:35:09.84 CC.seek :open @14:35:14.29 Forearm.seek :retract @14:35:15.27 Elbow.reconfigure :tight @14:35:15.32 Elbow.seek 2 @14:35:17.02 Elbow.reconfigure :limp @14:35:17.51 Forearm.seek 2 @14:35:18.65 Elbow.hold @14:35:18.71 Elbow.reconfigure :tight @14:35:18.75 Hand.open @14:35:19.57 Forearm.force -20 @14:35:19.60 Forearm.seek 2 + 314 counts @14:35:19.92 Forearm.reconfigure :tight @14:35:19.96 Forearm.seek 2 + 700 counts @14:35:20.33 SE.seek :down @14:35:21.41 SE.stop @14:35:21.53
Forearm.reconfigure :loose @14:35:21.57 Forearm.seek :retract @14:35:22.49 PSV.dial :air @14:35:23.23 PS.seek 2.5ml @14:35:49.48 delay 1 second @14:35:50.49 PSV.dial :mixloop @14:35:50.92 delay 1 second @14:35:51.93 PSV.dial :air @14:35:52.37 delay 1 second @14:35:53.39 PSV.dial :mixloop @14:35:53.83 delay 1 second @14:35:54.84 PSV.dial :air @14:35:55.28 delay 1 second @14:35:56.30 PS.seek 0ml @14:36:22.48 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:36:22.65 Elbow.seek FlushPuck::Garage @14:36:24.43 Elbow.reconfigure :loose @14:36:24.52 Forearm.seek :garage @14:36:25.63 Hand.close @14:36:26.32 Forearm.seek :retract @14:36:27.38 Elbow.reconfigure :tight @14:36:27.42 Elbow.seek PC @14:36:28.65 Elbow.reconfigure :loose @14:36:28.74 Forearm.reconfigure :tight @14:36:28.78 Forearm.seek PC @14:36:30.13 PC.seek :closed @14:36:34.01 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.36 @14:36:34.08 Hand.open @14:36:34.89 Forearm.reconfigure :loose @14:36:34.93 Forearm.seek :retract @14:36:36.02 PRV.dial :air @14:36:36.46 PSV.dial :mixloop @14:36:36.98 PS.seek 0.3ml @14:36:40.91 PSV.dial PSR @14:36:41.95 Pulling 0.4ml of PSR.air @14:36:41.96 PSR.select :air @14:36:41.99 PS.seek 0.7ml @14:36:46.88 delay 3 seconds @14:36:49.90 PSR.select :air @14:36:49.92 PS.seek 2.5ml @14:37:08.98 delay 3 seconds @14:37:11.99 PSV.dial :mixloop @14:37:12.98 PTV.dial :mixing @14:37:13.70 PBV.dial :waste2 @14:37:14.58 PS.seek 0ml @14:37:40.79 Garaging FlushPuck in Processing Clamp @14:37:40.86 Elbow.reconfigure :tight @14:37:40.90 Elbow.seek PC @14:37:41.01 Elbow.reconfigure :loose @14:37:41.10 Forearm.seek PC @14:37:42.16 Hand.close @14:37:42.71 PC.seek :open @14:37:46.65 Forearm.seek :retract @14:37:47.66 Elbow.reconfigure :tight @14:37:47.70 Elbow.seek :garage @14:37:49.07 Elbow.reconfigure :loose @14:37:49.16 Forearm.reconfigure :tight @14:37:49.20 Forearm.seek :garage @14:37:50.30 Hand.open @14:37:51.14 Forearm.reconfigure :loose @14:37:51.65 Forearm.seek :retract @14:37:52.72 Loading da puck from tube 3 into Processing Clamp @14:37:52.94 SC.reconfigure :tight @14:37:52.98 SC.seek 3 @14:37:56.52 SC.configure :limp @14:37:56.72 Elbow.reconfigure :tight @14:37:56.76 Elbow.seek 3 @14:37:58.66 Forearm.reconfigure :tight @14:37:58.70 Forearm.seek 3 + 700 counts @14:37:59.73 SE.reconfigure :pick @14:37:59.78 SE.seek :beamBreak @14:38:02.45 SE.configure :default @14:38:02.59 Elbow.seek 3 @14:38:02.76 Forearm.seek 3 + 300 counts @14:38:03.03 Forearm.reconfigure :loose @14:38:03.07 Hand.close @14:38:03.71 SE.seek :down @14:38:03.91
Forearm.seek :retract @14:38:04.86 Elbow.seek PC @14:38:06.08 SE.stop @14:38:07.62
Elbow.reconfigure :loose @14:38:07.71 Forearm.reconfigure :tight @14:38:07.75 Forearm.seek PC @14:38:09.07 PC.seek :closed @14:38:12.95 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.32 @14:38:12.96 Hand.open @14:38:13.76 Forearm.reconfigure :loose @14:38:13.80 Forearm.seek :retract @14:38:14.84 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @14:38:14.85 PH.seek 28C,5:00 @14:39:03.05 PH.hold 2:00:00,28C @14:39:03.10 delay 1:30 @14:40:33.13 PSR.select :air @14:40:33.15 PSV.dial PSR @14:40:34.21 Pulling 0.5ml of PSR.wash @14:40:34.22 PSR.select :wash @14:40:34.34 PS.seek 0.5ml @14:40:40.32 delay 5 seconds @14:40:45.34 PSR.select :air @14:40:45.45 PS.seek 2ml @14:41:01.53 PTV.dial :puck @14:41:02.24 PSV.dial PTV @14:41:02.97 PBV.dial :waste1 @14:41:04.31 PS.seek 1.5ml @14:41:10.28 delay 3 seconds @14:41:13.30 PBV.dial between PSV and waste1 @14:41:13.67 PS.seek 0ml @14:41:29.75 delay 5:00 @14:46:29.77 recoverPSRreagent08 @14:46:29.78 PTV.dial :puck @14:46:29.79 PBV.dial :waste1 @14:46:30.14 delay 30 seconds @14:47:00.16 PBV.dial between PSV and waste1 @14:47:00.53 PSR.select :air @14:47:00.54 PSV.dial PSR @14:47:01.28 PS.reconfigure :fast @14:47:01.32 PS.seek 2.5ml @14:47:24.15 PSV.dial PTV @14:47:24.98 delay 5 seconds @14:47:30.00 PSV.dial PSR @14:47:30.82 delay 5 seconds @14:47:35.84 PSV.dial PTV @14:47:37.03 delay 5 seconds @14:47:42.04 PSV.dial :air @14:47:42.77 PS.seek 0ml @14:48:05.49 PTV.dial :PRVmixing,via: PRV @14:48:06.76 PSV.dial :mixloop @14:48:07.21 PRV.dial :ab1 @14:48:07.97 PS.reconfigure :slow @14:48:08.01 PS.seek 0.15ml @14:48:11.52 delay 2 seconds @14:48:13.54 CBV.dial PRV @14:48:13.58
PRV.dial CBV @14:48:14.11 PS.seek 0.34ml @14:48:18.43 delay 2 seconds @14:48:20.45 CBV.dial between PRV and PSV @14:48:20.75 PRV.dial :air @14:48:21.20 PS.seek 0.9ml @14:48:33.05 PRV.dial :waste2 @14:48:33.78 PS.seek 0.5ml @14:48:42.40 addPMLReagent08 @14:48:42.41 PSV.dial :air @14:48:42.86 PS.reconfigure :default @14:48:42.90 PS.seek 2ml @14:48:58.99 PSV.dial :PML @14:48:59.44 PTV.dial :mixing @14:49:00.15 PBV.dial :waste1 @14:49:00.57 PS.seek 1.85ml @14:49:02.93 PBV.dial between PSV and waste1 @14:49:03.30 PS.seek 0ml @14:49:22.90 delay 2 seconds @14:49:24.91 Beginning samplePassC @14:49:24.93 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:49:25.09 Elbow.reconfigure :tight @14:49:25.13 Elbow.seek FlushPuck::Garage @14:49:26.50 Elbow.reconfigure :loose @14:49:26.59 Forearm.seek :garage @14:49:27.71 Hand.close @14:49:28.42 Forearm.seek :retract @14:49:29.53 Elbow.reconfigure :tight @14:49:29.57 Elbow.seek CC @14:49:32.42 Elbow.reconfigure :loose @14:49:32.51 Forearm.reconfigure :tight @14:49:32.55 Forearm.seek CC @14:49:33.84 CC.seek :closed @14:49:38.08 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.79 @14:49:38.14 Hand.open @14:49:38.99 Forearm.reconfigure :loose @14:49:39.03 Forearm.seek :retract @14:49:40.02 PSV.dial PSR @14:49:41.08 Pulling 1.5ml of PSR.flush PSR.select :flush @14:49:41.20 PS.seek 1.5ml @14:49:57.27 delay 5 seconds @14:50:02.29 PSV.dial :waste1 @14:50:02.85 PS.seek 0ml @14:50:18.89 PSV.dial PSR @14:50:19.32 PSR.select :air @14:50:19.43 PS.seek 2.5ml @14:50:45.59 PRV.dial CBV,avoiding: :ab2 @14:50:45.61
CBV.dial PRV @14:50:47.19 PSV.dial :mixloop @14:50:48.30 CTV.dial :air,avoiding: :intake @14:50:48.31 PTV.dial :PRVmixing @14:50:49.03 PS.seek 0ml @14:51:15.17 PSV.dial :air @14:51:15.63 PS.seek 2.5ml @14:51:41.77 PSV.dial :mixloop @14:51:42.21 PS.seek 0ml @14:52:08.36 PSV.dial :air @14:52:08.81 PS.seek 2.5ml @14:52:35.05 PSV.dial :mixloop @14:52:35.96 PS.seek 0ml @14:53:02.15 Garaging FlushPuck in Collection Clamp @14:53:02.22 Elbow.reconfigure :tight @14:53:02.26 Elbow.seek CC @14:53:02.37 Elbow.reconfigure :loose @14:53:02.46 Forearm.seek CC @14:53:03.41 Hand.close @14:53:04.18 CC.seek :open @14:53:08.51 Forearm.seek :retract @14:53:09.52 Elbow.reconfigure :tight @14:53:09.56 Elbow.seek :garage @14:53:12.42 Elbow.reconfigure :loose @14:53:12.51 Forearm.reconfigure :tight @14:53:12.55 Forearm.seek :garage @14:53:13.69 Hand.open @14:53:14.48 Forearm.reconfigure :loose @14:53:14.53 Forearm.seek :retract @14:53:15.58 delayUntil "14:57:24.90" @14:53:15.61 Whole Cell Archive sampling 1000ml @14:53:15.62 Loading wcr puck from tube 3 into Collection Clamp @14:53:16.05 Elbow.reconfigure :tight @14:53:16.10 Elbow.seek 3 @14:53:18.12 Forearm.reconfigure :tight @14:53:18.16 Forearm.seek 3 + 700 counts @14:53:19.24 SE.reconfigure :pick @14:53:19.29 SE.seek :beamBreak @14:53:22.05 SE.configure :default @14:53:22.20 Elbow.seek 3 @14:53:22.52 Forearm.seek 3 + 300 counts @14:53:22.75 Forearm.reconfigure :loose @14:53:22.79 Hand.close @14:53:23.44 SE.seek :down @14:53:23.62 Forearm.seek :retract @14:53:24.59 Elbow.seek CC @14:53:25.81 SE.stop @14:53:26.19 Elbow.reconfigure :loose @14:53:26.28 Forearm.reconfigure :tight @14:53:26.32 Forearm.seek CC @14:53:27.56 CC.seek :closed @14:53:31.79 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.65 @14:53:31.80 Hand.open @14:53:32.61 Forearm.reconfigure :loose @14:53:32.65 Forearm.seek :retract @14:53:33.65 collectSample 1000ml w/bubble point of 35psi @14:53:33.66 Priming 7ml @14:53:33.67 Intake.select :close @14:53:33.68 Exhaust.select :close @14:53:33.69 SS.hold @14:53:33.89 Calibrating pressure sensors @14:53:33.90 CTV.dial :air @14:53:33.91 SSV.dial :spit,avoiding: :exhaust @14:53:33.93 SS.reconfigure :fast @14:53:33.97 SS.seek 5ml @14:53:36.77 SSV.dial CBV @14:53:36.80 CBV.dial SSV @14:53:38.12 delay 3.8 seconds @14:53:42.04 SSV.dial :spit @14:53:43.37 SS.seek 0ml @14:53:46.51 SSV.dial CBV @14:53:47.82 CTV.dial :intake @14:53:49.03 SSV.dial CBV @14:53:49.05 CBV.dial SSV @14:53:49.07 Allowing 40s for 7.0ml of remaining 7.0ml @14:53:49.08 Intake.select :open @14:53:51.24 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:53:51.26 SS.reconfigure :inhaling 2.14ml/s @14:53:51.30 SS.seek 7ml @14:53:57.14 Stroke completed -- waiting for pressure to equalize ... @14:53:57.16 SS.reconfigure :exhale @14:53:57.20 SS.hold @14:54:19.61 Intake.select :close @14:54:19.75 SSV.dial :exhaust @14:54:20.49 Exhaust.select :open @14:54:20.60 SS.seek 0ml @14:54:24.85 Exhaust.select :close @14:54:24.95 Sampling 1000.0ml @14:54:24.96 Intake.select :close @14:54:24.98 Exhaust.select :close @14:54:24.99 SS.reconfigure :default @14:54:25.03 SS.hold @14:54:25.22 CTV.dial :intake @14:54:25.24 SSV.dial CBV @14:54:25.26 CBV.dial SSV @14:54:26.02 Allowing 130s for 25.0ml of remaining 1000.0ml @14:54:26.04 Intake.select :open @14:54:28.19 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:54:28.20 SS.reconfigure :inhaling 2.14ml/s @14:54:28.25 SS.seek 25ml @14:54:33.29 Vacuum too high -- waiting up to 40 seconds @14:54:33.30 SS.reconfigure :exhale @14:54:33.35 SS.hold @14:54:36.64 Intake @13.2psia, vacuum from 7.7 to 10.7psi @14:54:36.66 SS.reconfigure :inhaling 1.93ml/s @14:54:36.70 SS.seek 25ml @14:54:38.82 Vacuum too high -- waiting up to 40 seconds @14:54:38.84 SS.reconfigure :exhale @14:54:38.88 SS.hold @14:54:41.53 Intake @13.2psia, vacuum from 7.7 to 10.7psi @14:54:41.55 SS.reconfigure :inhaling 1.74ml/s @14:54:41.59 SS.seek 25ml @14:54:43.79 Vacuum too high -- waiting up to 40 seconds @14:54:43.81 SS.reconfigure :exhale @14:54:43.85 SS.hold @14:54:46.09 SS.reconfigure :inhaling 1.57ml/s @14:54:46.14 SS.seek 25ml @14:54:48.26 Vacuum too high -- waiting up to 40 seconds @14:54:48.27 SS.reconfigure :exhale @14:54:48.32 SS.hold @14:54:50.50 Intake @13.5psia, vacuum from 8.0 to 11.0psi @14:54:50.52 SS.reconfigure :inhaling 1.41ml/s @14:54:50.56 SS.seek 25ml @14:54:52.60 Vacuum too high -- waiting up to 40 seconds @14:54:52.62 SS.reconfigure :exhale @14:54:52.66 SS.hold @14:54:54.38 SS.reconfigure :inhaling 1.27ml/s @14:54:54.42 SS.seek 25ml @14:54:56.29 Vacuum too high -- waiting up to 40 seconds @14:54:56.31 SS.reconfigure :exhale @14:54:56.35 SS.hold @14:54:58.46 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:54:58.47 SS.reconfigure :inhaling 1.14ml/s @14:54:58.52 SS.seek 25ml @14:55:00.86 Vacuum too high -- waiting up to 40 seconds @14:55:00.87 SS.reconfigure :exhale @14:55:00.91 SS.hold @14:55:02.57 Intake @13.4psia, vacuum from 7.9 to 10.9psi @14:55:02.58 SS.reconfigure :inhaling 1.03ml/s @14:55:02.63 SS.seek 25ml @14:55:05.20 Vacuum too high -- waiting up to 40 seconds @14:55:05.21 SS.reconfigure :exhale @14:55:05.25 SS.hold @14:55:07.31 Intake @13.8psia, vacuum from 8.3 to 11.3psi @14:55:07.32 SS.reconfigure :inhaling 0.93ml/s @14:55:07.36 SS.seek 25ml @14:55:09.79 Vacuum too high -- waiting up to 40 seconds @14:55:09.80 SS.reconfigure :exhale @14:55:09.85 SS.hold @14:55:11.50 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:55:11.52 SS.reconfigure :inhaling 0.84ml/s @14:55:11.56 SS.seek 25ml @14:55:14.76 Vacuum too high -- waiting up to 40 seconds @14:55:14.77 SS.reconfigure :exhale @14:55:14.82 SS.hold @14:55:16.86 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:55:16.88 SS.reconfigure :inhaling 0.76ml/s @14:55:16.93 SS.seek 25ml @14:55:20.28 Stroke completed -- waiting for pressure to equalize ... @14:55:20.29 SS.reconfigure :exhale @14:55:20.34 SS.hold @14:55:23.80 Intake.select :close @14:55:23.94 SSV.dial :exhaust @14:55:24.69 Exhaust.select :open @14:55:24.82 SS.seek 0ml @14:55:33.77 Exhaust.select :close @14:55:33.87 SSV.dial CBV @14:55:33.90 CBV.dial SSV @14:55:34.66 Allowing 130s for 25.0ml of remaining 975.0ml @14:55:34.67 Intake.select :open @14:55:36.83 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:55:36.84 SS.reconfigure :inhaling 0.76ml/s @14:55:36.89 SS.seek 25ml @14:55:41.45 Vacuum too high -- waiting up to 40 seconds @14:55:41.46 SS.reconfigure :exhale @14:55:41.50 SS.hold @14:55:42.46 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:55:42.48 SS.reconfigure :inhaling 0.69ml/s @14:55:42.52 SS.seek 25ml @14:55:46.74 Vacuum too high -- waiting up to 40 seconds @14:55:46.76 SS.reconfigure :exhale @14:55:46.80 SS.hold @14:55:47.20 Intake @12.4psia, vacuum from 6.9 to 9.9psi @14:55:47.21 SS.reconfigure :inhaling 0.61ml/s @14:55:47.26 SS.seek 25ml @14:55:53.42 Vacuum too high -- waiting up to 40 seconds @14:55:53.91 SS.reconfigure :exhale @14:55:53.96 SS.hold @14:55:55.09 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:55:55.11 SS.reconfigure :inhaling 0.56ml/s @14:55:55.16 SS.seek 25ml @14:56:05.01 Vacuum too high -- waiting up to 40 seconds @14:56:05.02 SS.reconfigure :exhale @14:56:05.07 SS.hold @14:56:05.73 Intake @12.6psia, vacuum from 7.1 to 10.1psi @14:56:05.75 SS.reconfigure :inhaling 0.50ml/s @14:56:05.80 SS.seek 25ml @14:56:27.13 Vacuum too high -- waiting up to 40 seconds @14:56:27.14 SS.reconfigure :exhale @14:56:27.18 SS.hold @14:56:28.72 Intake @13.3psia, vacuum from 7.8 to 10.8psi @14:56:28.74 SS.reconfigure :inhaling 0.46ml/s @14:56:28.78 SS.seek 25ml @14:56:32.02 Stroke completed -- waiting for pressure to equalize ... @14:56:32.03 SS.reconfigure :exhale @14:56:32.07 SS.hold @14:56:34.56 Intake.select :close @14:56:34.70 SSV.dial :exhaust @14:56:35.42 Exhaust.select :open @14:56:35.53 SS.seek 0ml @14:56:44.92 Exhaust.select :close @14:56:45.03 SSV.dial CBV @14:56:45.05 CBV.dial SSV @14:56:45.79 Allowing 130s for 25.0ml of remaining 950.0ml @14:56:45.80 Intake.select :open @14:56:47.96 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:56:47.97 SS.reconfigure :inhaling 0.46ml/s @14:56:48.02 SS.seek 25ml @14:57:24.91 recoverPRVreagent08 @14:57:24.93 PTV.dial :mixing @14:57:25.65 PBV.dial :waste1 @14:57:26.01 delay 30 seconds @14:57:44.84 Stroke completed -- waiting for pressure to equalize ... @14:57:44.85 SS.reconfigure :exhale @14:57:44.90 SS.hold @14:57:48.34 Intake.select :close @14:57:48.48 SSV.dial :exhaust @14:57:49.20 Exhaust.select :open @14:57:49.31 SS.seek 0ml @14:57:56.03 PBV.dial between PSV and waste1 @14:57:56.40 PSV.dial :air @14:57:56.86 PS.reconfigure :fast @14:57:56.90 PS.seek 2.5ml @14:57:58.78 Exhaust.select :close @14:57:58.89 SSV.dial CBV @14:57:58.91 CBV.dial SSV @14:57:59.66 Allowing 130s for 25.0ml of remaining 925.0ml @14:57:59.67 Intake.select :open @14:58:01.82 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:58:01.84 SS.reconfigure :inhaling 0.46ml/s @14:58:01.89 SS.seek 25ml @14:58:20.01 PSV.dial :mixloop @14:58:20.46 delay 2 seconds @14:58:22.48 PSV.dial :air @14:58:22.93 delay 2 seconds @14:58:24.95 PSV.dial :mixloop @14:58:25.40 delay 2 seconds @14:58:27.42 PSV.dial :air @14:58:27.88 PS.seek 0ml @14:58:50.76 washCycleDA #1 of 2 @14:58:50.77 PSR.select :air @14:58:50.78 PSV.dial PSR @14:58:52.12 Pulling 0.5ml of PSR.wash @14:58:52.13 PSR.select :wash @14:58:52.24 PS.reconfigure :default @14:58:52.29 PS.seek 0.5ml @14:58:58.20 delay 5 seconds @14:58:59.07 Stroke completed -- waiting for pressure to equalize ... @14:58:59.09 SS.reconfigure :exhale @14:58:59.13 SS.hold @14:59:03.21 PSR.select :air @14:59:03.33 PS.seek 2ml @14:59:04.37 Intake.select :close @14:59:04.52 SSV.dial :exhaust @14:59:05.26 Exhaust.select :open @14:59:05.37 SS.seek 0ml @14:59:14.68 Exhaust.select :close @14:59:14.79 SSV.dial CBV @14:59:14.81 CBV.dial SSV @14:59:15.56 Allowing 130s for 25.0ml of remaining 900.0ml @14:59:15.57 Intake.select :open @14:59:17.73 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:59:17.74 SS.reconfigure :inhaling 0.46ml/s @14:59:17.79 SS.seek 25ml @14:59:19.32 PTV.dial :puck @14:59:20.05 PSV.dial PTV @14:59:20.77 PBV.dial :waste1 @14:59:21.19 PS.seek 1.5ml @14:59:27.04 delay 3 seconds @14:59:30.06 PBV.dial between PSV and waste1 @14:59:30.44 PS.seek 0ml @14:59:46.45 delay 2:00 @14:59:59.10 Vacuum too high -- waiting up to 40 seconds @14:59:59.11 SS.reconfigure :exhale @14:59:59.15 SS.hold @15:00:02.15 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:00:02.17 SS.reconfigure :inhaling 0.41ml/s @15:00:02.21 SS.seek 25ml @15:00:19.96 Stroke completed -- waiting for pressure to equalize ... @15:00:19.97 SS.reconfigure :exhale @15:00:20.02 SS.hold @15:00:24.12 Intake.select :close @15:00:24.27 SSV.dial :exhaust @15:00:24.99 Exhaust.select :open @15:00:25.10 SS.seek 0ml @15:00:34.51 Exhaust.select :close @15:00:34.61 SSV.dial CBV @15:00:34.64 CBV.dial SSV @15:00:35.40 Allowing 130s for 25.0ml of remaining 875.0ml @15:00:35.41 Intake.select :open @15:00:37.57 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:00:37.58 SS.reconfigure :inhaling 0.41ml/s @15:00:37.62 SS.seek 25ml @15:01:40.83 Stroke completed -- waiting for pressure to equalize ... @15:01:40.85 SS.reconfigure :exhale @15:01:40.89 SS.hold @15:01:46.46 recoverPSRreagent08 @15:01:46.47 PTV.dial :puck @15:01:46.48 PBV.dial :waste1 @15:01:46.85 delay 30 seconds @15:01:47.28 Intake.select :close @15:01:47.42 SSV.dial :exhaust @15:01:48.15 Exhaust.select :open @15:01:48.26 SS.seek 0ml @15:01:57.61 Exhaust.select :close @15:01:57.72 SSV.dial CBV @15:01:57.77 CBV.dial SSV @15:01:58.48 Allowing 130s for 25.0ml of remaining 850.0ml @15:01:58.49 Intake.select :open @15:02:00.65 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:02:00.67 SS.reconfigure :inhaling 0.41ml/s @15:02:00.71 SS.seek 25ml @15:02:16.87 PBV.dial between PSV and waste1 @15:02:17.24 PSR.select :air @15:02:17.65 PSV.dial PSR @15:02:18.59 PS.reconfigure :fast @15:02:18.63 PS.seek 2.5ml @15:02:37.72 Vacuum too high -- waiting up to 40 seconds @15:02:37.73 SS.reconfigure :exhale @15:02:37.77 SS.hold @15:02:40.28 Intake @13.8psia, vacuum from 8.3 to 11.3psi @15:02:40.30 SS.reconfigure :inhaling 0.37ml/s @15:02:40.34 SS.seek 25ml @15:02:41.47 PSV.dial PTV @15:02:42.19 delay 5 seconds @15:02:47.21 PSV.dial PSR @15:02:47.94 delay 5 seconds @15:02:52.96 PSV.dial PTV @15:02:53.69 delay 5 seconds @15:02:58.71 PSV.dial :air @15:02:59.45 PS.seek 0ml @15:03:11.16 Stroke completed -- waiting for pressure to equalize ... @15:03:11.17 SS.reconfigure :exhale @15:03:11.21 SS.hold @15:03:16.78 Intake.select :close @15:03:16.93 SSV.dial :exhaust @15:03:17.68 Exhaust.select :open @15:03:17.79 SS.seek 0ml @15:03:22.60 washCycleDA #2 of 2 @15:03:22.61 PSR.select :air @15:03:22.62 PSV.dial PSR @15:03:23.96 Pulling 0.5ml of PSR.wash @15:03:23.97 PSR.select :wash @15:03:24.08 PS.reconfigure :default @15:03:24.12 PS.seek 0.5ml @15:03:26.82 Exhaust.select :close @15:03:26.92 SSV.dial CBV @15:03:26.95 CBV.dial SSV @15:03:27.72 Allowing 130s for 25.0ml of remaining 825.0ml @15:03:27.73 Intake.select :open @15:03:29.88 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:03:29.90 SS.reconfigure :inhaling 0.37ml/s @15:03:29.94 SS.seek 25ml @15:03:30.07 delay 5 seconds @15:03:35.08 PSR.select :air @15:03:35.19 PS.seek 2ml @15:03:51.22 PTV.dial :puck @15:03:51.23 PSV.dial PTV @15:03:51.95 PBV.dial :waste1 @15:03:52.38 PS.seek 1.5ml @15:03:58.28 delay 3 seconds @15:04:01.30 PBV.dial between PSV and waste1 @15:04:01.68 PS.seek 0ml @15:04:17.66 delay 2:00 @15:04:42.77 Stroke completed -- waiting for pressure to equalize ... @15:04:42.78 SS.reconfigure :exhale @15:04:42.82 SS.hold @15:04:51.37 Intake.select :close @15:04:51.52 SSV.dial :exhaust @15:04:52.27 Exhaust.select :open @15:04:52.38 SS.seek 0ml @15:05:01.87 Exhaust.select :close @15:05:01.98 SSV.dial CBV @15:05:02.02 CBV.dial SSV @15:05:02.75 Allowing 130s for 25.0ml of remaining 800.0ml @15:05:02.76 Intake.select :open @15:05:04.92 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:05:04.94 SS.reconfigure :inhaling 0.37ml/s @15:05:04.98 SS.seek 25ml @15:05:47.65 Vacuum too high -- waiting up to 40 seconds @15:05:47.66 SS.reconfigure :exhale @15:05:47.70 SS.hold @15:05:51.41 Intake @15.0psia, vacuum from 9.5 to 12.5psi @15:05:51.43 SS.reconfigure :inhaling 0.33ml/s @15:05:51.48 SS.seek 25ml @15:06:17.67 recoverPSRreagent08 @15:06:17.69 PTV.dial :puck @15:06:17.70 PBV.dial :waste1 @15:06:18.07 delay 30 seconds @15:06:26.90 Stroke completed -- waiting for pressure to equalize ... @15:06:26.92 SS.reconfigure :exhale @15:06:26.96 SS.hold @15:06:34.34 Intake.select :close @15:06:34.48 SSV.dial :exhaust @15:06:35.22 Exhaust.select :open @15:06:35.33 SS.seek 0ml @15:06:44.71 Exhaust.select :close @15:06:44.82 SSV.dial CBV @15:06:44.86 CBV.dial SSV @15:06:45.59 Allowing 130s for 25.0ml of remaining 775.0ml @15:06:45.61 Intake.select :open @15:06:47.76 Intake @14.9psia, vacuum from 9.4 to 12.4psi @15:06:47.78 SS.reconfigure :inhaling 0.33ml/s @15:06:47.82 SS.seek 25ml @15:06:48.09 PBV.dial between PSV and waste1 @15:06:48.45 PSR.select :air @15:06:48.47 PSV.dial PSR @15:06:49.20 PS.reconfigure :fast @15:06:49.25 PS.seek 2.5ml @15:07:12.40 PSV.dial PTV @15:07:13.14 delay 5 seconds @15:07:18.16 PSV.dial PSR @15:07:18.89 delay 5 seconds @15:07:23.90 PSV.dial PTV @15:07:24.74 delay 5 seconds @15:07:29.76 PSV.dial :air @15:07:30.52 PS.seek 0ml @15:07:53.40 getPMLReagent08 @15:07:53.41 PRV.dial :air @15:07:53.87 delay 5 seconds @15:07:58.89 PTV.dial :PRVmixing @15:08:00.17 delay 5 seconds @15:08:05.18 PRV.dial :ab2 @15:08:05.63 PTV.dial :PRVmixing @15:08:05.65 PSV.dial :mixloop @15:08:06.09 PS.reconfigure :default @15:08:06.14 PS.seek 0.3ml @15:08:09.96 delay 2 seconds @15:08:11.62 Stroke completed -- waiting for pressure to equalize ... @15:08:11.63 SS.reconfigure :exhale @15:08:11.67 SS.hold @15:08:11.97 PRV.dial :air @15:08:12.56 PS.seek 0.55ml @15:08:15.96 addPMLReagent08 @15:08:15.97 PSV.dial :air @15:08:16.47 PS.seek 2ml @15:08:22.75 Intake.select :close @15:08:22.89 SSV.dial :exhaust @15:08:23.64 Exhaust.select :open @15:08:23.75 SS.seek 0ml @15:08:32.05 PSV.dial :PML @15:08:32.49 PTV.dial :mixing @15:08:33.21 PBV.dial :waste1 @15:08:33.27 Exhaust.select :close @15:08:33.37 SSV.dial CBV @15:08:33.40 CBV.dial SSV @15:08:33.63 PS.seek 1.85ml @15:08:34.15 Allowing 130s for 25.0ml of remaining 750.0ml @15:08:34.17 Intake.select :open @15:08:36.15 PBV.dial between PSV and waste1 @15:08:36.73 Intake @15.0psia, vacuum from 9.5 to 12.5psi @15:08:36.75 SS.reconfigure :inhaling 0.33ml/s @15:08:36.80 SS.seek 25ml @15:08:37.02 PS.seek 0ml @15:08:56.62 delay 2 seconds @15:08:58.63 delay 8:00 @15:09:22.45 Vacuum too high -- waiting up to 40 seconds @15:09:22.47 SS.reconfigure :exhale @15:09:22.51 SS.hold @15:09:27.25 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:09:27.27 SS.reconfigure :inhaling 0.30ml/s @15:09:27.31 SS.seek 25ml @15:10:10.35 Stroke completed -- waiting for pressure to equalize ... @15:10:10.36 SS.reconfigure :exhale @15:10:10.40 SS.hold @15:10:21.45 Intake.select :close @15:10:21.60 SSV.dial :exhaust @15:10:22.35 Exhaust.select :open @15:10:22.46 SS.seek 0ml @15:10:31.93 Exhaust.select :close @15:10:32.03 SSV.dial CBV @15:10:32.06 CBV.dial SSV @15:10:32.82 Allowing 130s for 25.0ml of remaining 725.0ml @15:10:32.83 Intake.select :open @15:10:34.99 Intake @15.0psia, vacuum from 9.5 to 12.5psi @15:10:35.00 SS.reconfigure :inhaling 0.30ml/s @15:10:35.05 SS.seek 25ml @15:11:36.39 Vacuum too high -- waiting up to 40 seconds @15:11:36.40 SS.reconfigure :exhale @15:11:36.44 SS.hold @15:11:41.96 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:11:41.97 SS.reconfigure :inhaling 0.27ml/s @15:11:42.02 SS.seek 25ml @15:12:18.72 Stroke completed -- waiting for pressure to equalize ... @15:12:18.73 SS.reconfigure :exhale @15:12:18.77 SS.hold @15:12:29.80 Intake.select :close @15:12:29.94 SSV.dial :exhaust @15:12:30.69 Exhaust.select :open @15:12:30.80 SS.seek 0ml @15:12:40.24 Exhaust.select :close @15:12:40.34 SSV.dial CBV @15:12:40.38 CBV.dial SSV @15:12:41.13 Allowing 130s for 25.0ml of remaining 700.0ml @15:12:41.14 Intake.select :open @15:12:43.29 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:12:43.31 SS.reconfigure :inhaling 0.27ml/s @15:12:43.36 SS.seek 25ml @15:14:23.79 Vacuum too high -- waiting up to 40 seconds @15:14:23.80 SS.reconfigure :exhale @15:14:23.84 SS.hold @15:14:27.28 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:14:27.29 SS.reconfigure :inhaling 0.24ml/s @15:14:27.33 SS.seek 25ml @15:14:34.65 Stroke completed -- waiting for pressure to equalize ... @15:14:34.66 SS.reconfigure :exhale @15:14:34.70 SS.hold @15:14:45.73 Intake.select :close @15:14:45.88 SSV.dial :exhaust @15:14:46.62 Exhaust.select :open @15:14:46.74 SS.seek 0ml @15:14:56.22 Exhaust.select :close @15:14:56.32 SSV.dial CBV @15:14:56.36 CBV.dial SSV @15:14:57.09 Allowing 130s for 25.0ml of remaining 675.0ml @15:14:57.11 Intake.select :open @15:14:59.27 Intake @15.4psia, vacuum from 9.9 to 12.9psi @15:14:59.28 SS.reconfigure :inhaling 0.24ml/s @15:14:59.33 SS.seek 25ml @15:16:58.65 recoverPRVreagent08 @15:16:58.67 PTV.dial :mixing @15:16:58.68 PBV.dial :waste1 @15:16:59.04 delay 30 seconds @15:16:59.16 Stroke completed -- waiting for pressure to equalize ... @15:16:59.18 SS.reconfigure :exhale @15:16:59.22 SS.hold @15:17:07.11 SS.reconfigure :default @15:17:07.16 SS.hold @15:17:07.27 Intake.select :close @15:17:07.42 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 350.0ml @15:17:07.43 SSV.dial :exhaust @15:17:08.18 Exhaust.select :open @15:17:08.29 SS.reconfigure :exhale @15:17:08.33 SS.seek 0ml @15:17:17.93 Exhaust.select :close @15:17:18.04 Sampled 350.0ml @15:17:18.06 Drying puck before fixing for 20:00 @15:17:18.07 CBV.dial :waste2b @15:17:18.80 delay 1:00 @15:17:29.06 PBV.dial between PSV and waste1 @15:17:29.42 PSV.dial :air @15:17:29.87 PS.reconfigure :fast @15:17:29.92 PS.seek 2.5ml @15:17:52.94 PSV.dial :mixloop @15:17:53.38 delay 2 seconds @15:17:55.40 PSV.dial :air @15:17:55.86 delay 2 seconds @15:17:57.87 PSV.dial :mixloop @15:17:58.34 delay 2 seconds @15:18:00.36 PSV.dial :air @15:18:00.81 PS.seek 0ml @15:18:18.82 CSV.dial :air @15:18:19.27 CS.reconfigure :fast @15:18:19.32 CS.seek 10ml @15:18:23.70 washCycleDA #1 of 3 @15:18:23.71 PSR.select :air @15:18:23.72 PSV.dial PSR @15:18:25.17 Pulling 0.5ml of PSR.wash @15:18:25.18 PSR.select :wash @15:18:25.29 PS.reconfigure :default @15:18:25.33 PS.seek 0.5ml @15:18:31.29 delay 5 seconds @15:18:36.31 PSR.select :air @15:18:36.42 PS.seek 2ml @15:18:42.46 Pumping wcr puck from tube 3 up to 35psi... @15:18:42.95 CBV.dial SSV @15:18:44.27 CBV.dial between CSV and waste2b @15:18:45.20 CSV.dial between air and CTV @15:18:45.53 CS.seek 7.305ml @15:18:52.30 CTV.dial CSV,avoiding: :air @15:18:52.33 CSV.dial CTV @15:18:52.44 PTV.dial :puck @15:18:53.11 CS.reconfigure :pressurize @15:18:53.18 PSV.dial PTV @15:18:53.21 CS.seek 0ml @15:18:53.98 PBV.dial :waste1 @15:18:54.41 PS.seek 1.5ml @15:19:00.29 delay 3 seconds @15:19:03.31 PBV.dial between PSV and waste1 @15:19:03.69 PS.seek 0ml @15:19:06.88 CBV.dial :waste2b @15:19:07.21 CS.reconfigure :default @15:19:07.25 CS.seek 0ml @15:19:13.17 delay 1:00 @15:19:19.70 delay 2:00 @15:20:13.19 CTV.dial :air,avoiding: :intake @15:20:14.47 Fix #1 of 2 (with RNAlater) for 10:00 @15:20:14.48 CSV.dial CSR @15:20:14.99 Pulling 2ml of CSR.RNAlater @15:20:15.00 CSR.select :RNAlater @15:20:15.11 CS.seek 2ml @15:20:20.92 delay 5 seconds @15:20:25.94 CSR.select :air @15:20:26.05 CS.reconfigure :fast @15:20:26.10 CS.seek 10ml @15:20:44.51 Pumping wcr puck from tube 3 up to 35psi... @15:20:44.53 CBV.dial SSV @15:20:45.81 CBV.dial between CSV and waste2b @15:20:46.74 CSV.dial between air and CTV @15:20:47.35 CS.seek 7.181ml @15:20:54.42 CTV.dial CSV,avoiding: :air @15:20:54.45 CSV.dial CTV @15:20:55.80 CS.reconfigure :pressurize @15:20:55.84 CS.seek 0ml @15:21:12.28 delay 10:00 @15:21:19.72 recoverPSRreagent08 @15:21:19.73 PTV.dial :puck @15:21:19.74 PBV.dial :waste1 @15:21:20.11 delay 30 seconds @15:21:50.14 PBV.dial between PSV and waste1 @15:21:50.49 PSR.select :air @15:21:50.51 PSV.dial PSR @15:21:51.24 PS.reconfigure :fast @15:21:51.29 PS.seek 2.5ml @15:22:14.19 PSV.dial PTV @15:22:14.92 delay 5 seconds @15:22:19.94 PSV.dial PSR @15:22:20.67 delay 5 seconds @15:22:25.69 PSV.dial PTV @15:22:26.43 delay 5 seconds @15:22:31.44 PSV.dial :air @15:22:32.18 PS.seek 0ml @15:22:54.94 washCycleDA #2 of 3 @15:22:54.95 PSR.select :air @15:22:54.97 PSV.dial PSR @15:22:56.29 Pulling 0.5ml of PSR.wash @15:22:56.30 PSR.select :wash @15:22:56.41 PS.reconfigure :default @15:22:56.45 PS.seek 0.5ml @15:23:02.38 delay 5 seconds @15:23:07.40 PSR.select :air @15:23:07.51 PS.seek 2ml @15:23:23.50 PTV.dial :puck @15:23:23.51 PSV.dial PTV @15:23:24.22 PBV.dial :waste1 @15:23:24.65 PS.seek 1.5ml @15:23:30.56 delay 3 seconds @15:23:33.58 PBV.dial between PSV and waste1 @15:23:33.96 PS.seek 0ml @15:23:49.97 delay 2:00 @15:25:50.00 recoverPSRreagent08 @15:25:50.01 PTV.dial :puck @15:25:50.02 PBV.dial :waste1 @15:25:50.39 delay 30 seconds @15:26:20.41 PBV.dial between PSV and waste1 @15:26:20.76 PSR.select :air @15:26:20.78 PSV.dial PSR @15:26:21.52 PS.reconfigure :fast @15:26:21.56 PS.seek 2.5ml @15:26:44.90 PSV.dial PTV @15:26:45.64 delay 5 seconds @15:26:50.66 PSV.dial PSR @15:26:51.39 delay 5 seconds @15:26:56.41 PSV.dial PTV @15:26:57.14 delay 5 seconds @15:27:02.16 PSV.dial :air @15:27:02.89 PS.seek 0ml @15:27:25.65 washCycleDA #3 of 3 @15:27:25.66 PSR.select :air @15:27:25.68 PSV.dial PSR @15:27:27.00 Pulling 0.5ml of PSR.wash @15:27:27.01 PSR.select :wash @15:27:27.12 PS.reconfigure :default @15:27:27.16 PS.seek 0.5ml @15:27:33.09 delay 5 seconds @15:27:38.11 PSR.select :air @15:27:38.22 PS.seek 2ml @15:27:54.34 PTV.dial :puck @15:27:54.35 PSV.dial PTV @15:27:55.06 PBV.dial :waste1 @15:27:55.49 PS.seek 1.5ml @15:28:01.34 delay 3 seconds @15:28:04.35 PBV.dial between PSV and waste1 @15:28:04.74 PS.seek 0ml @15:28:20.77 delay 2:00 @15:30:20.80 recoverPSRreagent08 @15:30:20.81 PTV.dial :puck @15:30:20.82 PBV.dial :waste1 @15:30:21.18 delay 30 seconds @15:30:51.20 PBV.dial between PSV and waste1 @15:30:51.55 PSR.select :air @15:30:51.57 PSV.dial PSR @15:30:52.31 PS.reconfigure :fast @15:30:52.35 PS.seek 2.5ml @15:31:12.30 CBV.dial :waste2b @15:31:12.63 CS.reconfigure :default @15:31:12.67 CS.seek 0ml @15:31:15.27 PSV.dial PTV @15:31:15.68 delay 1:00 @15:31:16.00 delay 5 seconds @15:31:21.02 PSV.dial PSR @15:31:21.76 delay 5 seconds @15:31:26.77 PSV.dial PTV @15:31:27.51 delay 5 seconds @15:31:32.52 PSV.dial :air @15:31:33.24 PS.seek 0ml @15:31:55.89 Gate.power :camera,:ON @15:31:55.91 Mixing DA Substrates @15:31:55.92 PSR.select :air @15:31:55.93 PSV.dial PSR @15:31:57.48 Pulling 0.25ml of PSR.sub1 @15:31:57.52 PSR.select :sub1 @15:31:57.82 PS.reconfigure :default @15:31:57.99 PS.seek 0.25ml @15:32:01.40 delay 5 seconds @15:32:06.47 Pulling 0.25ml of PSR.sub2 @15:32:06.48 PSR.select :sub2 @15:32:06.60 PS.seek 0.5ml @15:32:10.02 delay 5 seconds @15:32:15.04 PSR.select :air @15:32:15.15 PS.seek 2ml @15:32:15.70 CTV.dial :air,avoiding: :intake @15:32:17.00 Fix #2 of 2 (with RNAlater) for 10:00 @15:32:17.01 CSV.dial CSR @15:32:17.50 Pulling 2ml of CSR.RNAlater @15:32:17.51 CSR.select :RNAlater @15:32:17.62 CS.seek 2ml @15:32:23.50 delay 5 seconds @15:32:28.52 CSR.select :air @15:32:28.63 CS.reconfigure :fast @15:32:28.67 CS.seek 10ml @15:32:31.11 PTV.dial :puck @15:32:31.12 PSV.dial PTV @15:32:31.84 PBV.dial :waste1 @15:32:32.25 PS.seek 1.5ml @15:32:38.17 delay 3 seconds @15:32:41.19 PBV.dial between PSV and waste1 @15:32:41.56 PS.seek 0ml @15:32:47.84 Pumping wcr puck from tube 3 up to 35psi... @15:32:47.85 CBV.dial SSV @15:32:49.14 CBV.dial between CSV and waste2b @15:32:50.08 CSV.dial between air and CTV @15:32:50.69 CS.seek 7.03ml @15:32:57.77 delay 10 seconds @15:32:58.18 CTV.dial CSV,avoiding: :air @15:32:58.21 CSV.dial CTV @15:32:59.56 CS.reconfigure :pressurize @15:32:59.61 CS.seek 0ml @15:33:07.79 recoverPSRreagent08 @15:33:07.80 PTV.dial :puck @15:33:07.81 PBV.dial :waste1 @15:33:08.16 delay 30 seconds @15:33:15.80 delay 10:00 @15:33:38.17 PBV.dial between PSV and waste1 @15:33:38.53 PSR.select :air @15:33:38.55 PSV.dial PSR @15:33:39.78 PS.reconfigure :fast @15:33:39.84 PS.seek 2.5ml @15:34:02.98 PSV.dial PTV @15:34:03.72 delay 5 seconds @15:34:08.74 PSV.dial PSR @15:34:09.47 delay 5 seconds @15:34:14.49 PSV.dial PTV @15:34:15.22 delay 5 seconds @15:34:20.24 PSV.dial :air @15:34:20.97 PS.seek 0ml @15:34:44.05 Additional air clear @15:34:44.06 PSR.select :air @15:34:44.07 PSV.dial PSR @15:34:45.41 Pulling 2ml of PSR.air @15:34:45.42 PSR.select :air @15:34:45.44 PS.reconfigure :default @15:34:45.49 PS.seek 2ml @15:35:06.64 delay 5 seconds @15:35:11.66 PTV.dial :puck @15:35:11.68 PSV.dial PTV @15:35:12.40 PBV.dial :waste1 @15:35:12.82 PS.seek 1.5ml @15:35:18.67 delay 3 seconds @15:35:21.69 PBV.dial between PSV and waste1 @15:35:22.05 delay 1 second @15:35:23.06 PSV.dial :waste1 @15:35:24.10 PS.seek 0ml @15:35:40.28 Processing Puck Heater at 28C before imaging @15:35:40.29 PH.coast @15:35:40.41 Imaging da puck in Processing Clamp while storing it to tube 2 @15:35:40.57 SC.reconfigure :tight @15:35:40.62 SC.seek 2 @15:35:44.24 SC.configure :limp @15:35:44.44 Elbow.reconfigure :tight @15:35:44.48 Elbow.seek 2 @15:35:46.39 Forearm.reconfigure :tight @15:35:46.44 Forearm.seek 2 + 700 counts @15:35:47.53 SE.reconfigure :pick @15:35:47.60 SE.seek :beamBreak @15:35:49.12 SE.configure :default @15:35:49.27 Elbow.reconfigure :limp @15:35:49.31 Forearm.reconfigure :loose @15:35:49.35 Forearm.seek 2 @15:35:49.75 Elbow.hold @15:35:49.81 Elbow.reconfigure :tight @15:35:49.90 SE.seek 24.35 Pucks below Top Plate @15:35:50.39 Forearm.force -20 @15:35:50.43 Forearm.seek 2 + 313 counts @15:35:50.74 Forearm.reconfigure :tight @15:35:50.78 Forearm.seek 2 + 700 counts @15:35:51.08 SE.seek 25.35 Pucks below Top Plate @15:35:51.64 Forearm.reconfigure :loose @15:35:51.68 Forearm.seek :retract @15:35:52.57 Elbow.seek PC @15:35:55.22 Elbow.reconfigure :loose @15:35:55.31 Forearm.seek PC @15:35:56.40 Hand.close @15:35:57.10 PC.seek :open @15:36:01.14 Forearm.seek :retract @15:36:02.19 Elbow.reconfigure :tight @15:36:02.23 Elbow.seek 2 @15:36:04.92 Forearm.reconfigure :tight @15:36:04.96 Forearm.seek 2 + 700 counts @15:36:06.03 Hand.open @15:36:06.78 SE.seek 25 Pucks below Top Plate @15:36:07.06 SE.reconfigure :seal @15:36:07.10 SE.seek 23.89 Pucks below Top Plate @15:36:07.64 Lights, camera, ACTION! @15:36:07.67 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23mar1715h1000ml.tif @15:36:27.15 Waiting >20 seconds for da to relinquish FlushPuck @15:36:47.00 SX MX5/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 35.773 seconds /var/log/esp/da23mar1715h1000ml.tif: TIFF Upload Complete @15:36:47.02 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23mar1715h1000ml15s.tif @15:37:02.76 SX MX5/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23mar1715h1000ml15s.tif: TIFF Upload Complete @15:37:02.78 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da23mar1715h1000ml25s.tif @15:37:28.52 SX MX5/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da23mar1715h1000ml25s.tif: TIFF Upload Complete @15:37:28.53 Gate.power :camera,:OFF @15:37:28.61 DAfans.email "35.773 sec auto-exposure",Subject: "da23mar1715h1000ml images!" @15:37:28.85 Elbow.seek 2 @15:37:29.19 Forearm.seek 2 + 700 counts @15:37:29.29 SE.reconfigure :gently @15:37:29.33 SE.seek 24.61 Pucks below Top Plate @15:37:30.11 Elbow.reconfigure :limp @15:37:30.15 Forearm.reconfigure :loose @15:37:30.19 Forearm.seek 2 @15:37:30.65 Elbow.hold @15:37:30.72 Elbow.reconfigure :tight @15:37:30.77 SE.reconfigure :default @15:37:30.83 SE.seek 24.79 Pucks below Top Plate @15:37:31.30 Forearm.force -20 @15:37:31.34 Forearm.seek 2 + 316 counts @15:37:31.63 Forearm.reconfigure :tight @15:37:31.67 Forearm.seek 2 + 700 counts @15:37:32.00 SE.seek :down @15:37:33.18 Forearm.reconfigure :loose @15:37:33.27 Forearm.seek :retract @15:37:33.31 SE.stop @15:37:34.56 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:37:35.01 Elbow.seek FlushPuck::Garage @15:37:36.90 Elbow.reconfigure :loose @15:37:37.08 Forearm.seek :garage @15:37:38.27 Hand.close @15:37:39.22 Forearm.seek :retract @15:37:40.39 Elbow.reconfigure :tight @15:37:40.47 Elbow.seek PC @15:37:41.87 Elbow.reconfigure :loose @15:37:42.07 Forearm.reconfigure :tight @15:37:42.16 Forearm.seek PC @15:37:43.83 PC.seek :closed @15:37:47.84 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.39 @15:37:47.98 Hand.open @15:37:48.85 Forearm.reconfigure :loose @15:37:48.96 Forearm.seek :retract @15:37:50.06 PSR.select :air @15:37:50.12 PSV.dial PSR @15:37:50.75 Pulling 0.5ml of PSR.flush @15:37:50.77 PSR.select :flush @15:37:50.95 PS.seek 0.5ml @15:37:56.95 delay 5 seconds @15:38:01.98 PSR.select :air @15:38:02.11 PS.seek 2.5ml @15:38:02.33 upsync @15:38:23.23 delay 1 second @15:38:24.24 PRV.dial :waste2,avoiding: :ab2 @15:38:26.07 PTV.dial :PRVmixing @15:38:27.32 PSV.dial :mixloop @15:38:28.33 PS.seek :empty @15:38:54.54 PSR.select :air @15:38:54.56 PSV.dial PSR @15:38:55.61 Pulling 0.5ml of PSR.flush @15:38:55.62 PSR.select :flush @15:38:55.73 PS.seek 0.5ml @15:39:01.60 delay 5 seconds @15:39:06.62 PSR.select :air @15:39:06.73 PS.seek 2.5ml @15:39:27.92 delay 1 second @15:39:28.94 PBV.dial :waste1 @15:39:29.30 PTV.dial :mixing @15:39:30.02 PSV.dial :mixloop @15:39:31.02 PS.seek :empty @15:39:57.19 PSV.dial PSR @15:39:58.19 PSR.select :flush @15:39:58.31 PS.seek 1ml @15:40:09.38 PSR.select :air @15:40:09.49 PS.seek 2.5ml @15:40:25.53 PSV.dial PTV @15:40:26.33 PTV.dial :puck @15:40:27.04 PBV.dial :waste1 @15:40:27.06 PS.seek 0ml @15:40:53.28 PSV.dial PSR @15:40:54.13 PSR.select :flush @15:40:54.24 PS.seek 0.5ml @15:41:00.31 PSR.select :air @15:41:00.43 PS.seek 2.5ml @15:41:21.60 PSV.dial PTV @15:41:22.43 PBV.dial PSV @15:41:22.98 PS.seek 0ml @15:41:49.16 PSV.dial PBV @15:41:49.60 PS.seek 2.5ml @15:42:15.75 PSV.dial :waste1 @15:42:16.48 PS.seek 0ml @15:42:42.69 PSV.dial PSR @15:42:43.14 PSR.select :air @15:42:43.17 PS.seek 2.5ml @15:43:09.37 PSV.dial PTV @15:43:10.09 PBV.dial PSV @15:43:10.11 PS.seek 0ml @15:43:15.82 CBV.dial :waste2b @15:43:16.14 CS.reconfigure :default @15:43:16.18 CS.seek 0ml @15:43:18.88 delay 1:00 @15:43:36.44 PSV.dial PBV @15:43:36.90 PS.seek 2.5ml @15:44:03.03 PSV.dial :waste1 @15:44:03.75 PS.seek 0ml @15:44:18.90 CTV.dial :air,avoiding: :intake @15:44:20.24 Storing wcr puck in Collection Clamp to tube 2 @15:44:20.61 Elbow.reconfigure :tight @15:44:20.66 Elbow.seek 2 @15:44:23.36 Forearm.reconfigure :tight @15:44:23.41 Forearm.seek 2 + 700 counts @15:44:24.41 SE.reconfigure :pick @15:44:24.46 SE.seek :beamBreak @15:44:25.88 SE.configure :default @15:44:26.02 Elbow.reconfigure :limp @15:44:26.07 Forearm.reconfigure :loose @15:44:26.11 Forearm.seek 2 @15:44:26.54 Elbow.hold @15:44:26.61 Elbow.reconfigure :tight @15:44:26.71 SE.seek 25.35 Pucks below Top Plate @15:44:27.25 Forearm.force -20 @15:44:27.29 Forearm.seek 2 + 311 counts @15:44:27.63 Forearm.reconfigure :tight @15:44:27.68 Forearm.seek 2 + 700 counts @15:44:28.13 SE.seek 26.35 Pucks below Top Plate @15:44:28.70 Forearm.reconfigure :loose @15:44:28.74 Forearm.seek :retract @15:44:29.74 Elbow.seek CC @15:44:30.06 PSV.dial PSR @15:44:30.51 PSR.select :flush @15:44:30.63 PS.seek 1ml @15:44:31.37 Elbow.reconfigure :loose @15:44:31.46 Forearm.seek CC @15:44:32.40 Hand.close @15:44:33.09 CC.seek :open @15:44:37.53 Forearm.seek :retract @15:44:38.53 Elbow.reconfigure :tight @15:44:38.58 Elbow.seek 2 @15:44:40.26 Elbow.reconfigure :limp @15:44:40.31 Forearm.seek 2 @15:44:41.89 Elbow.hold @15:44:41.98 PSR.select :air @15:44:42.01 Elbow.reconfigure :tight @15:44:42.10 Hand.open @15:44:42.16 PS.seek 2.5ml @15:44:42.95 Forearm.force -20 @15:44:42.98 Forearm.seek 2 + 311 counts @15:44:43.35 Forearm.reconfigure :tight @15:44:43.40 Forearm.seek 2 + 700 counts @15:44:43.66 SE.seek :down @15:44:44.61 SE.stop @15:44:44.79 Forearm.reconfigure :loose @15:44:44.84 Forearm.seek :retract @15:44:45.75 Starting finalPurge @15:44:58.18 PSV.dial PTV @15:44:58.91 PTV.dial :puck @15:44:58.92 PBV.dial :waste1 @15:44:59.47 PS.seek 0ml @15:45:05.18
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:45:25.59 PSV.dial PSR @15:45:26.31 PSR.select :flush @15:45:26.42 PS.seek 0.5ml @15:45:32.37 PSR.select :air @15:45:32.48 PS.seek 2.5ml @15:45:53.62 PSV.dial PTV @15:45:54.34 PBV.dial PSV @15:45:54.89 PS.seek 0ml @15:46:21.24 PSV.dial PBV @15:46:21.70 PS.seek 2.5ml @15:46:47.87 PSV.dial :waste1 @15:46:48.59 PS.seek 0ml @15:47:14.81 PSV.dial PSR @15:47:15.26 PSR.select :air @15:47:15.28 PS.seek 2.5ml @15:47:41.40 PSV.dial PTV @15:47:42.12 PBV.dial PSV @15:47:42.14 PS.seek 0ml @15:48:08.37 PSV.dial PBV @15:48:08.83 PS.seek 2.5ml @15:48:34.99 PSV.dial :waste1 @15:48:35.72 PS.seek 0ml @15:49:01.87
Loading FlushPuck into Collection Clamp from Processing Clamp @15:49:02.04 Elbow.seek PC @15:49:04.61 Elbow.reconfigure :loose @15:49:04.69 Forearm.seek PC @15:49:05.74 Hand.close @15:49:06.32 PC.seek :open @15:49:10.29 Forearm.seek :retract @15:49:11.34 Elbow.reconfigure :tight @15:49:11.38 Elbow.seek CC @15:49:15.00 Elbow.reconfigure :loose @15:49:15.08 Forearm.reconfigure :tight @15:49:15.12 Forearm.seek CC @15:49:16.33 CC.seek :closed @15:49:20.56 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.82 @15:49:20.63 Hand.open @15:49:21.38 Forearm.reconfigure :loose @15:49:21.42 Forearm.seek :retract @15:49:22.41 Flushing the Collection Valve::Manifold @15:49:22.53 CSV.dial CSR @15:49:23.00 Pulling 5ml of CSR.flush @15:49:23.01 CSR.select :flush @15:49:23.12 CS.seek 5ml @15:49:36.56 delay 5 seconds @15:49:41.58 CSR.select :air @15:49:41.72 CS.reconfigure :fast @15:49:41.77 CS.seek 7.499ml @15:49:48.12 CSV.dial :waste @15:49:49.39 CS.reconfigure :default @15:49:49.43 CS.seek 0ml @15:50:09.56 Flushing lower manifold's waste1 port @15:50:09.67 CSV.dial :flush @15:50:10.11 CS.reconfigure :fast @15:50:10.15 CS.seek 5ml @15:50:22.09 delay 5 seconds @15:50:27.11 CSV.dial :air @15:50:27.61 CS.seek 7ml @15:50:32.87 CSV.dial CTV @15:50:32.90
CTV.dial CSV @15:50:34.23 CBV.dial :waste1 @15:50:34.99 CS.seek 0ml @15:50:51.10 Flushing lower manifold's waste2 port @15:50:51.21 CSV.dial :flush @15:50:52.05 CS.seek 5ml @15:51:03.90 delay 5 seconds @15:51:08.92 CSV.dial :air @15:51:09.42 CS.seek 7ml @15:51:14.78 CSV.dial CTV @15:51:14.80
CTV.dial CSV @15:51:15.25 CBV.dial :waste2 @15:51:16.27 CS.seek 0ml @15:51:32.37 Flushing lower manifold's waste2b port @15:51:32.49 CSV.dial :flush @15:51:33.22 CS.seek 5ml @15:51:45.15 delay 5 seconds @15:51:50.17 CSV.dial :air @15:51:50.68 CS.seek 7.001ml @15:51:55.90 CSV.dial CTV @15:51:55.95
CTV.dial CSV @15:51:56.36 CBV.dial :waste2b @15:51:57.36 CS.seek 0ml @15:52:13.53 Disinfecting external Intake with kill @15:52:13.64 CSV.dial CSR @15:52:14.13 Pulling 8ml of CSR.kill CSR.select :kill @15:52:14.25 CS.seek 8ml @15:52:32.53 delay 5 seconds @15:52:37.54 CSR.select :air @15:52:37.65 CS.seek 10ml @15:52:42.88 CSV.dial CBV @15:52:42.90
CBV.dial CSV @15:52:43.95 CTV.dial :intake @15:52:44.66 Intake.select :open @15:52:44.77 CS.seek 2ml @15:53:02.93 Intake.select :close @15:53:03.03 CTV.dial :air @15:53:03.78 CS.reconfigure :default @15:53:03.82 CS.seek 0ml @15:53:09.62 CSV.dial CSR @15:53:10.68 Pulling 5ml of CSR.flush @15:53:10.69 CSR.select :flush @15:53:10.81 CS.seek 5ml @15:53:24.24 delay 5 seconds @15:53:29.26 CSR.select :air @15:53:29.37 CS.seek 10ml @15:53:42.86 CSV.dial CBV @15:53:43.98 CS.seek 0ml @15:54:10.02 Garaging FlushPuck in Collection Clamp @15:54:10.09 Elbow.reconfigure :tight @15:54:10.13 Elbow.seek CC @15:54:10.33 Elbow.reconfigure :loose @15:54:10.42 Forearm.seek CC @15:54:11.37 Hand.close @15:54:11.94 CC.seek :open @15:54:16.25 Forearm.seek :retract @15:54:17.28 Elbow.reconfigure :tight @15:54:17.32 Elbow.seek :garage @15:54:20.17 Elbow.reconfigure :loose @15:54:20.26 Forearm.reconfigure :tight @15:54:20.30 Forearm.seek :garage @15:54:21.45 Hand.open @15:54:22.24 Forearm.reconfigure :loose @15:54:22.28 Forearm.seek :retract @15:54:23.36 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:21:11 @15:54:23.40 Asleep after completing DA_WCR_031723 assays @15:54:23.42 GoodNews.email "{1 => 33,\n 2 => 27,\n 3 => 6,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:50:15, 18.3C, 53% humidity, 15.5psia, 13.938V, 0.361A, 0.355A avg, 5.03W\nSampled 350.0ml",Subject: "Asleep after completing DA_WCR_031723 assays" @15:54:27.92 upsync @12:23:31.36UTC23-Mar-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @12:23:34.07 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @12:23:38.18 Polling Can every 10:00 @12:23:38.31 Cleanly exited at 12:23:03UTC23-Mar-23 @12:23:38.35 Loading /home/esp/esp2/mission/DA_WCR_032223.rb mission @12:23:38.40 GoodNews.email "Starting DA_WCR_032223 w/pucks in tube 3\n{1 => 33,\n 2 => 27,\n 3 => 6,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:23:38, 17.4C, 53% humidity, 15.5psia, 13.982V, 0.233A, 0.233A avg, 3.25W",Subject: "DA_WCR_032223 begun" @12:23:38.42
Preparing to sleep until + 00:00:05 @12:23:38.48 Asleep til + 00:00:05 @12:23:38.49 Continuing at 12:23 @12:23:38.78 Gate.power :core,:OFF @12:23:38.79 Gate.power :raw,:OFF @12:23:38.80 Shutdown @12:23:41.31 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_032223.rb:14\n{1 => 33,\n 2 => 27,\n 3 => 6,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:23:38, 17.3C, 53% humidity, 15.5psia, 13.977V, 0.239A, 0.233A avg, 3.34W",Subject: "Asleep until 12:23" @12:23:43.45
Powering core bus @12:23:45.81 upsync @12:23:48.81
Gate.power :raw,:ON @12:23:49.64 CS.configure :default @12:23:49.73 PS.configure :default @12:23:49.82 SS.configure :default @12:23:49.90 CC.configure :default @12:23:49.99 PC.configure :default @12:23:50.10 Forearm.configure :loose @12:23:50.18 Elbow.configure :loose @12:23:50.29 SC.configure :loose @12:23:50.37 SE.configure :default @12:23:50.41 CH.coast @12:23:50.47 CH.configure :default @12:23:50.50 PH.coast @12:23:50.55 PH.configure :default @12:23:50.58 All dwarf controllers running firmware version 3.85 @12:23:50.69 Valve.reset @12:23:50.88 Intake.select :close @12:23:50.93 Exhaust.select :close @12:23:50.99 CTV.dial :air @12:23:51.07 PSV.dial :air @12:23:51.12 CSV.dial :waste1 @12:23:51.25
Forearm.reconfigure :loose @12:23:51.27 PS.reconfigure :default @12:23:51.35
Forearm.home.seek :retract @12:23:51.40 PS.home.seek 0ml @12:23:51.61 SSV.dial :spit,avoiding: :exhaust @12:23:51.74 CS.reconfigure :fast @12:23:51.78 SS.reconfigure :default @12:23:51.85 CS.home.seek 0ml @12:23:51.88 SS.home.seek 0ml @12:23:52.11 Hand.close @12:23:52.14
Elbow.jog 1500 @12:23:52.56 Elbow.reconfigure :loose @12:23:52.60 Elbow.home.seek :clear @12:23:55.82 SE.reconfigure :default @12:23:55.86 SE.home.seek :down @12:23:56.47 SC.reconfigure :tight @12:23:56.52 SC.home.seek :initial @12:24:00.08 PS.stop @12:24:26.58 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @12:24:26.59
Retry #1 of 3 @12:24:26.65 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @12:24:31.03 SC.configure :limp @12:24:31.08 Forearm.seek :retract @12:24:31.14 Elbow.seek :hammer @12:24:32.42 Forearm.reconfigure :tight @12:24:32.46 Forearm.seek retracted @12:24:32.74 PC.reconfigure :default @12:24:32.76
CC.reconfigure :default @12:24:32.85 CC.home.seek :open @12:24:32.87 PC.home.seek :open @12:24:36.10
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @12:24:36.12 Notice.email "At 12:24:36 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_032223.rb:14\n{1 => 33,\n 2 => 27,\n 3 => 6,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:24:36, 17.4C, 53% humidity, 15.5psia, 13.997V, 0.208A, 0.208A avg, 2.91W",Subject: "Started DA_WCR_032223 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @12:24:36.13
Starting initialPurge @12:24:36.16 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:24:36.23 Hand.open @12:24:37.16 Elbow.reconfigure :tight @12:24:37.21 Elbow.seek FlushPuck::Garage @12:24:39.20 Elbow.reconfigure :loose @12:24:39.30 Forearm.reconfigure :loose @12:24:39.34 Forearm.seek :garage @12:24:39.96 upsync @12:24:40.50
Hand.close @12:24:41.40 Forearm.seek :retract @12:24:42.49 Elbow.reconfigure :tight @12:24:42.53 Elbow.seek CC @12:24:45.38 Elbow.reconfigure :loose @12:24:45.47 Forearm.reconfigure :tight @12:24:45.51 Forearm.seek CC @12:24:46.82 CC.seek :closed @12:24:51.05 Collection Clamp onSpring/offSpring for 8/25 samples, on/off torque 1.83 @12:24:51.12 Hand.open @12:24:51.96 Forearm.reconfigure :loose @12:24:52.00 Forearm.seek :retract @12:24:53.02 Flushing the Collection Valve::Manifold @12:24:53.15 CSV.dial CSR @12:24:54.46 Pulling 5ml of CSR.flush @12:24:54.47 CSR.select :flush @12:24:54.58 CS.reconfigure :default @12:24:54.62 CS.seek 5ml @12:25:08.06 delay 5 seconds @12:25:13.08 CSR.select :air @12:25:13.24 CS.reconfigure :fast @12:25:13.28 CS.seek 7.5ml @12:25:19.65 CSV.dial :waste @12:25:20.92 CS.reconfigure :default @12:25:20.97 CS.seek 0ml @12:25:40.72 Purging external Intake @12:25:40.83 CSV.dial :flush @12:25:41.27 CS.reconfigure :fast @12:25:41.31 CS.seek 10ml @12:26:04.28 delay 5 seconds @12:26:09.30 CSV.dial CBV @12:26:09.33
CBV.dial CSV @12:26:10.07 CTV.dial :intake @12:26:10.81 Intake.select :open @12:26:10.92 CS.seek 0ml @12:26:33.75 Intake.select :close @12:26:33.84 CTV.dial :air @12:26:34.59 Flushing upper manifold @12:26:34.71 CSV.dial :flush @12:26:35.44 CS.seek 5ml @12:26:47.34 delay 5 seconds @12:26:52.36 CSV.dial :air @12:26:52.85 CS.seek 7ml @12:26:58.09 CSV.dial CBV @12:26:58.11
CBV.dial CSV @12:26:59.14 CTV.dial :air @12:26:59.16 CS.seek 0ml @12:27:15.24 delay 7 seconds @12:27:22.27 CS.seek 3ml @12:27:29.81 CSV.dial :waste @12:27:30.25 CS.seek 0ml @12:27:37.75 CSV.dial :flush @12:27:38.19 CS.seek 3ml @12:27:45.68 delay 5 seconds @12:27:50.70 CSV.dial :air @12:27:51.32 CS.seek 5ml @12:27:56.55 CSV.dial :air @12:27:56.57 CS.seek 0ml @12:28:08.31 delay 7 seconds @12:28:15.33 CS.seek 3ml @12:28:22.77 CSV.dial :waste @12:28:23.48 CS.seek 0ml @12:28:30.87 Garaging FlushPuck in Collection Clamp @12:28:30.94 Elbow.reconfigure :tight @12:28:30.98 Elbow.seek CC @12:28:31.09 Elbow.reconfigure :loose @12:28:31.17 Forearm.seek CC @12:28:32.14 Hand.close @12:28:32.90 CC.seek :open @12:28:37.23 Forearm.seek :retract @12:28:38.33 Elbow.reconfigure :tight @12:28:38.38 Elbow.seek :garage @12:28:41.28 Elbow.reconfigure :loose @12:28:41.37 Forearm.reconfigure :tight @12:28:41.41 Forearm.seek :garage @12:28:42.52 Hand.open @12:28:43.69 Forearm.reconfigure :loose @12:28:43.80 Forearm.seek :retract @12:28:44.87 da sampling at most 1000ml, wcr at most 1000ml @12:28:44.89 DAfans.email "Started da in tube 3 at 12:28:44UTC23-Mar-23\n{1 => 33,\n 2 => 27,\n 3 => 6,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:24:36, 17.4C, 53% humidity, 15.5psia, 13.997V, 0.208A, 0.208A avg, 2.91W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @12:28:44.92
Loading sh1 puck from tube 3 into Collection Clamp @12:28:45.17 SC.reconfigure :tight @12:28:45.22 SC.seek 3 @12:28:48.66 upsync @12:28:55.06
SC.configure :limp @12:28:55.28 Elbow.reconfigure :tight @12:28:55.32 Elbow.seek 3 @12:28:57.29 Forearm.reconfigure :tight @12:28:57.34 Forearm.seek 3 + 700 counts @12:28:58.38 SE.reconfigure :pick @12:28:58.43 SE.seek :beamBreak @12:29:01.24 SE.configure :default @12:29:01.39 Elbow.seek 3 @12:29:01.56 Forearm.seek 3 + 300 counts @12:29:01.84 Forearm.reconfigure :loose @12:29:01.89 Hand.close @12:29:02.53 SE.seek :down @12:29:02.72
Forearm.seek :retract @12:29:03.65 Elbow.seek CC @12:29:04.98 SE.stop @12:29:05.23
Elbow.reconfigure :loose @12:29:05.33 Forearm.reconfigure :tight @12:29:05.37 Forearm.seek CC @12:29:06.65 CC.seek :closed @12:29:10.88 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.63 @12:29:10.89 Hand.open @12:29:11.71 Forearm.reconfigure :loose @12:29:11.75 Forearm.seek :retract @12:29:12.77 collectSample 1000ml w/bubble point of 24psi @12:29:12.78 Priming 7ml @12:29:12.79 Intake.select :close @12:29:12.80 Exhaust.select :close @12:29:12.81 SS.hold @12:29:13.01 Calibrating pressure and temperature sensors @12:29:13.04 CTV.dial :air @12:29:13.05 SSV.dial :spit,avoiding: :exhaust @12:29:13.07 SS.reconfigure :fast @12:29:13.11 SS.seek 5ml @12:29:15.83 SSV.dial CBV @12:29:15.86
CBV.dial SSV @12:29:17.21 delay 3.8 seconds @12:29:21.12 SSV.dial :spit @12:29:22.42 SS.seek 0ml @12:29:24.97 SSV.dial CBV @12:29:26.25 CTV.dial :intake @12:29:26.98 SSV.dial CBV @12:29:27.00
CBV.dial SSV @12:29:27.02 Allowing 40s for 7.0ml of remaining 7.0ml @12:29:27.03 Intake.select :open @12:29:29.18 Intake @15.2psia, vacuum from 9.7 to 12.7psi @12:29:29.20 SS.reconfigure :inhaling 2.14ml/s @12:29:29.24 SS.seek 7ml @12:29:36.56 Stroke completed -- waiting for pressure to equalize ... @12:29:36.57 SS.reconfigure :exhale @12:29:36.62 SS.hold @12:29:39.60 Intake.select :close @12:29:39.74 SSV.dial :exhaust @12:29:40.47 Exhaust.select :open @12:29:40.58 SS.seek 0ml @12:29:44.91 Exhaust.select :close @12:29:45.01 Sampling 1000.0ml @12:29:45.02 Intake.select :close @12:29:45.03 Exhaust.select :close @12:29:45.04 SS.reconfigure :default @12:29:45.08 SS.hold @12:29:45.26 CTV.dial :intake @12:29:45.28 SSV.dial CBV @12:29:45.31
CBV.dial SSV @12:29:46.05 Allowing 130s for 25.0ml of remaining 1000.0ml @12:29:46.06 Intake.select :open @12:29:48.21 Intake @15.2psia, vacuum from 9.7 to 12.7psi @12:29:48.23 SS.reconfigure :inhaling 2.14ml/s @12:29:48.27 SS.seek 25ml @12:29:52.57 Vacuum too high -- waiting up to 40 seconds @12:29:52.58 SS.reconfigure :exhale @12:29:52.62 SS.hold @12:29:53.30 Intake @11.4psia, vacuum from 5.9 to 8.9psi @12:29:53.31 SS.reconfigure :inhaling 1.93ml/s @12:29:53.87 Vacuum too high -- waiting up to 40 seconds @12:29:53.88 SS.reconfigure :exhale @12:29:53.92 SS.hold @12:29:54.71 Intake @13.7psia, vacuum from 8.2 to 11.2psi @12:29:54.72 SS.reconfigure :inhaling 1.74ml/s @12:29:54.77 SS.seek 25ml @12:29:57.04 Vacuum too high -- waiting up to 40 seconds @12:29:57.05 SS.reconfigure :exhale @12:29:57.09 SS.hold @12:29:58.26 SS.reconfigure :inhaling 1.57ml/s @12:29:58.31 SS.seek 25ml @12:30:00.79 Vacuum too high -- waiting up to 40 seconds @12:30:00.80 SS.reconfigure :exhale @12:30:00.84 SS.hold @12:30:01.94 Intake @14.0psia, vacuum from 8.5 to 11.5psi @12:30:01.95 SS.reconfigure :inhaling 1.41ml/s @12:30:02.00 SS.seek 25ml @12:30:04.04 Vacuum too high -- waiting up to 40 seconds @12:30:04.05 SS.reconfigure :exhale @12:30:04.09 SS.hold @12:30:05.16 Intake @14.0psia, vacuum from 8.5 to 11.5psi @12:30:05.17 SS.reconfigure :inhaling 1.27ml/s @12:30:05.22 SS.seek 25ml @12:30:07.79 Vacuum too high -- waiting up to 40 seconds @12:30:07.80 SS.reconfigure :exhale @12:30:07.84 SS.hold @12:30:08.87 Intake @13.8psia, vacuum from 8.3 to 11.3psi @12:30:08.89 SS.reconfigure :inhaling 1.14ml/s @12:30:08.93 SS.seek 25ml @12:30:11.73 Vacuum too high -- waiting up to 40 seconds @12:30:11.74 SS.reconfigure :exhale @12:30:11.78 SS.hold @12:30:12.86 Intake @13.8psia, vacuum from 8.3 to 11.3psi @12:30:12.88 SS.reconfigure :inhaling 1.03ml/s @12:30:12.92 SS.seek 25ml @12:30:15.45 Vacuum too high -- waiting up to 40 seconds @12:30:15.46 SS.reconfigure :exhale @12:30:15.50 SS.hold @12:30:16.46 Intake @13.6psia, vacuum from 8.1 to 11.1psi @12:30:16.47 SS.reconfigure :inhaling 0.93ml/s @12:30:16.52 SS.seek 25ml @12:30:20.48 Vacuum too high -- waiting up to 40 seconds @12:30:20.49 SS.reconfigure :exhale @12:30:20.53 SS.hold @12:30:21.48 Intake @13.5psia, vacuum from 8.0 to 11.0psi @12:30:21.50 SS.reconfigure :inhaling 0.84ml/s @12:30:21.54 SS.seek 25ml @12:30:26.60 Vacuum too high -- waiting up to 40 seconds @12:30:26.61 SS.reconfigure :exhale @12:30:26.65 SS.hold @12:30:27.60 Intake @13.3psia, vacuum from 7.8 to 10.8psi @12:30:27.62 SS.reconfigure :inhaling 0.76ml/s @12:30:27.66 SS.seek 25ml @12:30:28.75 Stroke completed -- waiting for pressure to equalize ... @12:30:28.76 SS.reconfigure :exhale @12:30:28.80 SS.hold @12:30:29.76 Intake.select :close @12:30:29.90 SSV.dial :exhaust @12:30:30.64 Exhaust.select :open @12:30:30.75 SS.seek 0ml @12:30:40.02 Exhaust.select :close @12:30:40.12 SSV.dial CBV @12:30:40.14
CBV.dial SSV @12:30:40.89 Allowing 130s for 25.0ml of remaining 975.0ml @12:30:40.90 Intake.select :open @12:30:43.06 Intake @14.9psia, vacuum from 9.4 to 12.4psi @12:30:43.07 SS.reconfigure :inhaling 0.76ml/s @12:30:43.12 SS.seek 25ml @12:31:02.19 Vacuum too high -- waiting up to 40 seconds @12:31:02.20 SS.reconfigure :exhale @12:31:02.25 SS.hold @12:31:02.92 Intake @13.6psia, vacuum from 8.1 to 11.1psi @12:31:02.93 SS.reconfigure :inhaling 0.69ml/s @12:31:02.97 SS.seek 25ml @12:31:20.30 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @12:31:20.34 SS.hold @12:31:22.29 Intake.select :close @12:31:22.43 SSV.dial :exhaust @12:31:23.17 Exhaust.select :open @12:31:23.28 SS.seek 0ml @12:31:32.81 Exhaust.select :close @12:31:32.91 SSV.dial CBV @12:31:32.95
CBV.dial SSV @12:31:33.67 Allowing 130s for 25.0ml of remaining 950.0ml @12:31:33.68 Intake.select :open @12:31:35.84 Intake @14.8psia, vacuum from 9.3 to 12.3psi @12:31:35.85 SS.reconfigure :inhaling 0.69ml/s @12:31:35.90 SS.seek 25ml @12:32:13.89 Stroke completed -- waiting for pressure to equalize ... @12:32:13.90 SS.reconfigure :exhale @12:32:13.94 SS.hold @12:32:16.47 Intake.select :close @12:32:16.61 SSV.dial :exhaust @12:32:17.34 Exhaust.select :open @12:32:17.45 SS.seek 0ml @12:32:26.36 Exhaust.select :close @12:32:26.46 SSV.dial CBV @12:32:26.48
CBV.dial SSV @12:32:27.23 Allowing 130s for 25.0ml of remaining 925.0ml @12:32:27.24 Intake.select :open @12:32:29.40 Intake @14.6psia, vacuum from 9.1 to 12.1psi @12:32:29.41 SS.reconfigure :inhaling 0.69ml/s @12:32:29.46 SS.seek 25ml @12:33:01.97 Vacuum too high -- waiting up to 40 seconds @12:33:01.98 SS.reconfigure :exhale @12:33:02.49 SS.hold @12:33:03.16 Intake @14.3psia, vacuum from 8.8 to 11.8psi @12:33:03.18 SS.reconfigure :inhaling 0.61ml/s @12:33:03.22 SS.seek 25ml @12:33:10.75 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @12:33:10.79 SS.hold @12:33:11.99 Intake.select :close @12:33:12.12 SSV.dial :exhaust @12:33:12.87 Exhaust.select :open @12:33:12.98 SS.seek 0ml @12:33:22.35 Exhaust.select :close @12:33:22.46 SSV.dial CBV @12:33:22.49
CBV.dial SSV @12:33:23.23 Allowing 130s for 25.0ml of remaining 900.0ml @12:33:23.24 Intake.select :open @12:33:25.40 Intake @14.5psia, vacuum from 9.0 to 12.0psi @12:33:25.41 SS.reconfigure :inhaling 0.61ml/s @12:33:25.46 SS.seek 25ml @12:34:07.62 Stroke completed -- waiting for pressure to equalize ... @12:34:07.63 SS.reconfigure :exhale @12:34:07.67 SS.hold @12:34:10.17 Intake.select :close @12:34:10.32 SSV.dial :exhaust @12:34:11.06 Exhaust.select :open @12:34:11.16 SS.seek 0ml @12:34:20.59 Exhaust.select :close @12:34:20.69 SSV.dial CBV @12:34:20.71
CBV.dial SSV @12:34:21.46 Allowing 130s for 25.0ml of remaining 875.0ml @12:34:21.47 Intake.select :open @12:34:23.63 Intake @14.4psia, vacuum from 8.9 to 11.9psi @12:34:23.65 SS.reconfigure :inhaling 0.61ml/s @12:34:23.69 SS.seek 25ml @12:35:05.79 Stroke completed -- waiting for pressure to equalize ... @12:35:05.80 SS.reconfigure :exhale @12:35:05.84 SS.hold @12:35:08.83 Intake.select :close @12:35:08.97 SSV.dial :exhaust @12:35:09.70 Exhaust.select :open @12:35:09.80 SS.seek 0ml @12:35:18.71 Exhaust.select :close @12:35:18.81 SSV.dial CBV @12:35:18.86
CBV.dial SSV @12:35:19.57 Allowing 130s for 25.0ml of remaining 850.0ml @12:35:19.58 Intake.select :open @12:35:21.74 Intake @14.3psia, vacuum from 8.8 to 11.8psi @12:35:21.75 SS.reconfigure :inhaling 0.61ml/s @12:35:21.80 SS.seek 25ml @12:36:04.79 Stroke completed -- waiting for pressure to equalize ... @12:36:04.80 SS.reconfigure :exhale @12:36:04.84 SS.hold @12:36:08.30 Intake.select :close @12:36:08.44 SSV.dial :exhaust @12:36:09.18 Exhaust.select :open @12:36:09.29 SS.seek 0ml @12:36:18.77 Exhaust.select :close @12:36:18.87 SSV.dial CBV @12:36:18.92
CBV.dial SSV @12:36:19.63 Allowing 130s for 25.0ml of remaining 825.0ml @12:36:19.64 Intake.select :open @12:36:21.80 Intake @14.2psia, vacuum from 8.7 to 11.7psi @12:36:21.81 SS.reconfigure :inhaling 0.61ml/s @12:36:21.85 SS.seek 25ml @12:36:48.31 Vacuum too high -- waiting up to 40 seconds @12:36:48.32 SS.reconfigure :exhale @12:36:48.36 SS.hold @12:36:49.64 Intake @13.0psia, vacuum from 7.5 to 10.5psi @12:36:49.66 SS.reconfigure :inhaling 0.56ml/s @12:36:49.70 SS.seek 25ml @12:37:07.61 Stroke completed -- waiting for pressure to equalize ... @12:37:07.62 SS.reconfigure :exhale @12:37:07.66 SS.hold @12:37:11.12 Intake.select :close @12:37:11.26 SSV.dial :exhaust @12:37:12.01 Exhaust.select :open @12:37:12.11 SS.seek 0ml @12:37:21.66 Exhaust.select :close @12:37:21.76 SSV.dial CBV @12:37:21.81
CBV.dial SSV @12:37:22.52 Allowing 130s for 25.0ml of remaining 800.0ml @12:37:22.53 Intake.select :open @12:37:24.69 Intake @14.2psia, vacuum from 8.7 to 11.7psi @12:37:24.70 SS.reconfigure :inhaling 0.56ml/s @12:37:24.75 SS.seek 25ml @12:38:08.18 Vacuum too high -- waiting up to 40 seconds @12:38:08.19 SS.reconfigure :exhale @12:38:08.23 SS.hold @12:38:10.28 Intake @13.3psia, vacuum from 7.8 to 10.8psi @12:38:10.29 SS.reconfigure :inhaling 0.50ml/s @12:38:10.34 SS.seek 25ml @12:38:14.05 Stroke completed -- waiting for pressure to equalize ... @12:38:14.06 SS.reconfigure :exhale @12:38:14.10 SS.hold @12:38:17.23 Intake.select :close @12:38:17.36 SSV.dial :exhaust @12:38:18.10 Exhaust.select :open @12:38:18.20 SS.seek 0ml @12:38:27.49 Exhaust.select :close @12:38:27.59 SSV.dial CBV @12:38:27.61
CBV.dial SSV @12:38:28.35 Allowing 130s for 25.0ml of remaining 775.0ml @12:38:28.36 Intake.select :open @12:38:30.51 Intake @14.2psia, vacuum from 8.7 to 11.7psi @12:38:30.53 SS.reconfigure :inhaling 0.50ml/s @12:38:30.57 SS.seek 25ml @12:39:22.31 Stroke completed -- waiting for pressure to equalize ... @12:39:22.32 SS.reconfigure :exhale @12:39:22.36 SS.hold @12:39:27.22 Intake.select :close @12:39:27.37 SSV.dial :exhaust @12:39:28.11 Exhaust.select :open @12:39:28.22 SS.seek 0ml @12:39:37.59 Exhaust.select :close @12:39:37.70 SSV.dial CBV @12:39:37.72
CBV.dial SSV @12:39:38.46 Allowing 130s for 25.0ml of remaining 750.0ml @12:39:38.47 Intake.select :open @12:39:40.62 Intake @14.2psia, vacuum from 8.7 to 11.7psi @12:39:40.63 SS.reconfigure :inhaling 0.50ml/s @12:39:40.68 SS.seek 25ml @12:40:29.27 Vacuum too high -- waiting up to 40 seconds @12:40:29.28 SS.reconfigure :exhale @12:40:29.32 SS.hold @12:40:32.76 Intake @14.9psia, vacuum from 9.4 to 12.4psi @12:40:32.77 SS.reconfigure :inhaling 0.46ml/s @12:40:32.81 SS.seek 25ml @12:40:36.61 Stroke completed -- waiting for pressure to equalize ... @12:40:36.62 SS.reconfigure :exhale @12:40:36.66 SS.hold @12:40:40.20 Intake.select :close @12:40:40.34 SSV.dial :exhaust @12:40:41.06 Exhaust.select :open @12:40:41.17 SS.seek 0ml @12:40:50.31 Exhaust.select :close @12:40:50.42 SSV.dial CBV @12:40:50.44
CBV.dial SSV @12:40:51.20 Allowing 130s for 25.0ml of remaining 725.0ml @12:40:51.21 Intake.select :open @12:40:53.37 Intake @14.3psia, vacuum from 8.8 to 11.8psi @12:40:53.38 SS.reconfigure :inhaling 0.46ml/s @12:40:53.43 SS.seek 25ml @12:41:51.07 Stroke completed -- waiting for pressure to equalize ... @12:41:51.08 SS.reconfigure :exhale @12:41:51.12 SS.hold @12:41:58.01 Intake.select :close @12:41:58.15 SSV.dial :exhaust @12:41:58.89 Exhaust.select :open @12:41:58.99 SS.seek 0ml @12:42:08.49 Exhaust.select :close @12:42:08.60 SSV.dial CBV @12:42:08.62
CBV.dial SSV @12:42:09.37 Allowing 130s for 25.0ml of remaining 700.0ml @12:42:09.38 Intake.select :open @12:42:11.54 Intake @14.4psia, vacuum from 8.9 to 11.9psi @12:42:11.55 SS.reconfigure :inhaling 0.46ml/s @12:42:11.60 SS.seek 25ml @12:42:46.70 Vacuum too high -- waiting up to 40 seconds @12:42:46.71 SS.reconfigure :exhale @12:42:46.75 SS.hold @12:42:49.64 Intake @14.6psia, vacuum from 9.1 to 12.1psi @12:42:49.66 SS.reconfigure :inhaling 0.41ml/s @12:42:49.70 SS.seek 25ml @12:43:14.37 Stroke completed -- waiting for pressure to equalize ... @12:43:14.38 SS.reconfigure :exhale @12:43:14.42 SS.hold @12:43:20.58 Intake.select :close @12:43:20.71 SSV.dial :exhaust @12:43:21.45 Exhaust.select :open @12:43:21.55 SS.seek 0ml @12:43:31.09 Exhaust.select :close @12:43:31.19 SSV.dial CBV @12:43:31.21
CBV.dial SSV @12:43:31.98 Allowing 130s for 25.0ml of remaining 675.0ml @12:43:31.99 Intake.select :open @12:43:34.14 Intake @14.5psia, vacuum from 9.0 to 12.0psi @12:43:34.16 SS.reconfigure :inhaling 0.41ml/s @12:43:34.20 SS.seek 25ml @12:44:37.93 Stroke completed -- waiting for pressure to equalize ... @12:44:37.94 SS.reconfigure :exhale @12:44:37.98 SS.hold @12:44:46.55 Intake.select :close @12:44:46.69 SSV.dial :exhaust @12:44:47.43 Exhaust.select :open @12:44:47.53 SS.seek 0ml @12:44:56.63 Exhaust.select :close @12:44:56.73 SSV.dial CBV @12:44:56.79
CBV.dial SSV @12:44:57.49 Allowing 130s for 25.0ml of remaining 650.0ml @12:44:57.50 Intake.select :open @12:44:59.66 Intake @14.5psia, vacuum from 9.0 to 12.0psi @12:44:59.68 SS.reconfigure :inhaling 0.41ml/s @12:44:59.72 SS.seek 25ml @12:45:37.45 Vacuum too high -- waiting up to 40 seconds @12:45:37.46 SS.reconfigure :exhale @12:45:37.50 SS.hold @12:45:41.56 Intake @14.7psia, vacuum from 9.2 to 12.2psi @12:45:41.57 SS.reconfigure :inhaling 0.37ml/s @12:45:41.62 SS.seek 25ml @12:46:11.67 Stroke completed -- waiting for pressure to equalize ... @12:46:11.68 SS.reconfigure :exhale @12:46:11.73 SS.hold @12:46:20.27 Intake.select :close @12:46:20.41 SSV.dial :exhaust @12:46:21.13 Exhaust.select :open @12:46:21.23 SS.seek 0ml @12:46:30.75 Exhaust.select :close @12:46:30.86 SSV.dial CBV @12:46:30.88
CBV.dial SSV @12:46:31.63 Allowing 130s for 25.0ml of remaining 625.0ml @12:46:31.64 Intake.select :open @12:46:33.79 Intake @14.5psia, vacuum from 9.0 to 12.0psi @12:46:33.81 SS.reconfigure :inhaling 0.37ml/s @12:46:33.85 SS.seek 25ml @12:47:44.16 Vacuum too high -- waiting up to 40 seconds @12:47:44.17 SS.reconfigure :exhale @12:47:44.21 SS.hold @12:47:49.74 Intake @14.7psia, vacuum from 9.2 to 12.2psi @12:47:49.75 SS.reconfigure :inhaling 0.33ml/s @12:47:49.80 SS.seek 25ml @12:47:52.80 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @12:47:52.84 SS.hold @12:47:58.07 Intake.select :close @12:47:58.20 SSV.dial :exhaust @12:47:58.96 Exhaust.select :open @12:47:59.07 SS.seek 0ml @12:48:08.16 Exhaust.select :close @12:48:08.26 SSV.dial CBV @12:48:08.28
CBV.dial SSV @12:48:09.02 Allowing 130s for 25.0ml of remaining 600.0ml @12:48:09.03 Intake.select :open @12:48:11.18 Intake @14.6psia, vacuum from 9.1 to 12.1psi @12:48:11.20 SS.reconfigure :inhaling 0.33ml/s @12:48:11.24 SS.seek 25ml @12:49:36.46 Stroke completed -- waiting for pressure to equalize ... @12:49:36.47 SS.reconfigure :exhale @12:49:36.52 SS.hold @12:49:47.65 Intake.select :close @12:49:47.79 SSV.dial :exhaust @12:49:48.53 Exhaust.select :open @12:49:48.63 SS.seek 0ml @12:49:57.70 Exhaust.select :close @12:49:57.80 SSV.dial CBV @12:49:57.82
CBV.dial SSV @12:49:58.58 Allowing 130s for 25.0ml of remaining 575.0ml @12:49:58.59 Intake.select :open @12:50:00.74 Intake @14.6psia, vacuum from 9.1 to 12.1psi @12:50:00.75 SS.reconfigure :inhaling 0.33ml/s @12:50:00.80 SS.seek 25ml @12:51:26.60 Stroke completed -- waiting for pressure to equalize ... @12:51:26.61 SS.reconfigure :exhale @12:51:26.65 SS.hold @12:51:41.01 Intake.select :close @12:51:41.15 SSV.dial :exhaust @12:51:41.88 Exhaust.select :open @12:51:41.99 SS.seek 0ml @12:51:51.38 Exhaust.select :close @12:51:51.49 SSV.dial CBV @12:51:51.52
CBV.dial SSV @12:51:52.24 Allowing 130s for 25.0ml of remaining 550.0ml @12:51:52.25 Intake.select :open @12:51:54.41 Intake @14.7psia, vacuum from 9.2 to 12.2psi @12:51:54.42 SS.reconfigure :inhaling 0.33ml/s @12:51:54.47 SS.seek 25ml @12:52:46.97 Vacuum too high -- waiting up to 40 seconds @12:52:46.98 SS.reconfigure :exhale @12:52:47.02 SS.hold @12:52:52.65 Intake @14.8psia, vacuum from 9.3 to 12.3psi @12:52:52.66 SS.reconfigure :inhaling 0.30ml/s @12:52:52.70 SS.seek 25ml @12:53:29.74 Stroke completed -- waiting for pressure to equalize ... @12:53:29.75 SS.reconfigure :exhale @12:53:29.79 SS.hold @12:53:42.38 Intake.select :close @12:53:42.89 SSV.dial :exhaust @12:53:43.72 Exhaust.select :open @12:53:43.83 SS.seek 0ml @12:53:52.93 Exhaust.select :close @12:53:53.03 SSV.dial CBV @12:53:53.05
CBV.dial SSV @12:53:53.80 Allowing 130s for 25.0ml of remaining 525.0ml @12:53:53.81 Intake.select :open @12:53:55.97 Intake @14.9psia, vacuum from 9.4 to 12.4psi @12:53:55.98 SS.reconfigure :inhaling 0.30ml/s @12:53:56.03 SS.seek 25ml @12:55:20.49 Vacuum too high -- waiting up to 40 seconds @12:55:20.50 SS.reconfigure :exhale @12:55:20.55 SS.hold @12:55:27.90 Intake @15.0psia, vacuum from 9.5 to 12.5psi @12:55:27.92 SS.reconfigure :inhaling 0.27ml/s @12:55:27.96 SS.seek 25ml @12:55:42.51 Stroke completed -- waiting for pressure to equalize ... @12:55:42.52 SS.reconfigure :exhale @12:55:42.57 SS.hold @12:55:53.73 Intake.select :close @12:55:53.87 SSV.dial :exhaust @12:55:54.59 Exhaust.select :open @12:55:54.70 SS.seek 0ml @12:56:03.81 Exhaust.select :close @12:56:03.92 SSV.dial CBV @12:56:03.94
CBV.dial SSV @12:56:04.67 Allowing 130s for 25.0ml of remaining 500.0ml @12:56:04.68 Intake.select :open @12:56:06.84 Intake @15.0psia, vacuum from 9.5 to 12.5psi @12:56:06.85 SS.reconfigure :inhaling 0.27ml/s @12:56:06.90 SS.seek 25ml @12:57:55.21 Stroke completed -- waiting for pressure to equalize ... @12:57:55.22 SS.reconfigure :exhale @12:57:55.27 SS.hold @12:58:13.59 Intake.select :close @12:58:13.73 SSV.dial :exhaust @12:58:14.47 Exhaust.select :open @12:58:14.57 SS.seek 0ml @12:58:24.15 Exhaust.select :close @12:58:24.26 SSV.dial CBV @12:58:24.28
CBV.dial SSV @12:58:25.01 Allowing 130s for 25.0ml of remaining 475.0ml @12:58:25.02 Intake.select :open @12:58:27.17 Intake @15.1psia, vacuum from 9.6 to 12.6psi @12:58:27.19 SS.reconfigure :inhaling 0.27ml/s @12:58:27.23 SS.seek 25ml @13:00:03.76 Vacuum too high -- waiting up to 40 seconds @13:00:03.77 SS.reconfigure :exhale @13:00:03.82 SS.hold @13:00:13.49 SS.reconfigure :inhaling 0.24ml/s @13:00:14.01 SS.seek 25ml @13:00:27.44 Stroke completed -- waiting for pressure to equalize ... @13:00:27.45 SS.reconfigure :exhale @13:00:27.50 SS.hold @13:00:35.03 SS.reconfigure :default @13:00:35.07 SS.hold @13:00:35.18 Intake.select :close @13:00:35.32 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 550.0ml @13:00:35.33 SSV.dial :exhaust @13:00:36.09 Exhaust.select :open @13:00:36.19 SS.reconfigure :exhale @13:00:36.23 SS.seek 0ml @13:00:45.87 Exhaust.select :close @13:00:45.97 Sampled 550.0ml @13:00:45.98 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:00:45.99 Intake.select :close @13:00:46.05 CSV.dial :waste @13:00:46.11 CS.seek 0ml @13:00:46.18 SSV.dial CBV,avoiding: :exhaust @13:00:46.21
CBV.dial SSV @13:00:46.26 CSV.dial :air @13:00:47.00 CS.seek 10ml @13:00:48.06
CTV.dial :air @13:00:48.80 delay 15 seconds @13:01:03.82 SSV.dial :spit,avoiding: :exhaust @13:01:05.15 CTV.dial CSV,avoiding: :intake @13:01:05.21
SS.seek 0ml @13:01:05.29 SSV.dial CBV,avoiding: :exhaust @13:01:10.62 CBV.dial between SSV and waste2 @13:01:10.99 CTV.dial CSV,avoiding: :air @13:01:11.02
CSV.dial CTV @13:01:11.57 CS.reconfigure :pressurize @13:01:11.62 CS.seek 0ml @13:01:30.22 BubblePoint: Intake @40.4psia, Outlet @16.0psia @13:01:30.23 CBV.dial SSV @13:01:30.54 SS.reconfigure :default @13:01:30.58 SS.seek 10ml @13:01:36.27 delay 15 seconds @13:01:51.39 Evacuated: Intake @16.0psia, Outlet @10.8psia @13:01:51.40 SSV.dial :spit,avoiding: :exhaust @13:01:52.73 SS.reconfigure :exhale @13:01:52.77 SS.seek 0ml @13:01:57.94 SS.reconfigure :fast @13:01:57.98 SS.seek 5ml @13:02:00.76 SSV.dial CBV,avoiding: :exhaust @13:02:00.78
CBV.dial SSV @13:02:02.12 CTV.dial :air,avoiding: :intake @13:02:03.41 CSV.dial CTV @13:02:03.45
SS.seek 10ml @13:02:04.44 CSV.dial :air @13:02:04.88 CS.reconfigure :fast @13:02:04.93 CS.seek 0ml @13:02:05.96
SSV.dial :spit,avoiding: :exhaust @13:02:07.28 SS.reconfigure :exhale @13:02:07.32 SS.seek 0ml @13:02:12.03 CS.stop @13:02:12.75
SS.reconfigure :fast @13:02:12.79 SS.seek 5ml @13:02:15.60 SSV.dial CBV,avoiding: :exhaust @13:02:15.62
CBV.dial SSV @13:02:17.95 SSV.dial :spit,avoiding: :exhaust @13:02:19.29 SS.reconfigure :default @13:02:19.34 SS.seek 0ml @13:02:22.86 SS.stop @13:02:22.90 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:02:22.91 Intake.select :close @13:02:22.98 CSV.dial :waste @13:02:23.09 SSV.dial CBV,avoiding: :exhaust @13:02:23.11
CBV.dial SSV @13:02:23.73 CS.seek 0ml @13:02:23.79 CSV.dial :air @13:02:24.43
CTV.dial :air @13:02:24.44 delay 15 seconds @13:02:24.65 CS.seek 10ml @13:02:39.46
SSV.dial :spit,avoiding: :exhaust @13:02:40.79 CTV.dial CSV,avoiding: :intake @13:02:40.82
SS.reconfigure :exhale @13:02:40.91 SS.seek 0ml @13:02:41.15 SSV.dial CBV,avoiding: :exhaust @13:02:47.84 CBV.dial between SSV and waste2 @13:02:48.66 CTV.dial CSV,avoiding: :air @13:02:48.69
CSV.dial CTV @13:02:49.15 CS.reconfigure :pressurize @13:02:49.19 CS.seek 0ml @13:03:10.51 BubblePoint: Intake @39.3psia, Outlet @15.3psia @13:03:10.52 CBV.dial SSV @13:03:10.84 SS.reconfigure :default @13:03:10.88 SS.seek 10ml @13:03:16.55 delay 15 seconds @13:03:31.66 Evacuated: Intake @18.2psia, Outlet @12.4psia @13:03:31.68 SSV.dial :spit,avoiding: :exhaust @13:03:32.99 SS.reconfigure :exhale @13:03:33.04 SS.seek 0ml @13:03:38.32 SS.reconfigure :fast @13:03:38.37 SS.seek 5ml @13:03:41.19 SSV.dial CBV,avoiding: :exhaust @13:03:41.22
CBV.dial SSV @13:03:42.54 CTV.dial :air,avoiding: :intake @13:03:43.82 CSV.dial CTV @13:03:43.85
SS.seek 10ml @13:03:44.85 CSV.dial :air @13:03:45.30 CS.reconfigure :fast @13:03:45.35 CS.seek 0ml @13:03:46.33
SSV.dial :spit,avoiding: :exhaust @13:03:47.65 SS.reconfigure :exhale @13:03:47.69 SS.seek 0ml @13:03:50.06 CS.stop @13:03:53.15
SS.reconfigure :fast @13:03:53.19 SS.seek 5ml @13:03:56.26 SSV.dial CBV,avoiding: :exhaust @13:03:56.28
CBV.dial SSV @13:03:58.60 SSV.dial :spit,avoiding: :exhaust @13:03:59.91 SS.reconfigure :default @13:03:59.96 SS.seek 0ml @13:04:03.03 SS.stop @13:04:03.07 Lysing: Adding 2.0ml AqMeOH @13:04:03.08 CBV.dial between CSV and waste1,avoiding: :DBV @13:04:04.55 CTV.dial CSV @13:04:05.84 CSV.dial CSR @13:04:06.62 Pulling 1ml of CSR.AqMeOH CSR.select :AqMeOH @13:04:06.73 CS.reconfigure :default @13:04:06.77 CS.seek 1ml @13:04:10.16 delay 5 seconds @13:04:15.18 CSR.select :air @13:04:15.33 CS.seek 6ml @13:04:28.84 delay 2 seconds @13:04:30.86 CSV.dial :waste2 @13:04:31.30 CS.reconfigure :fast @13:04:31.34 CS.seek 0ml @13:04:45.34 CSV.dial CSR @13:04:45.83 Pulling 2ml of CSR.AqMeOH @13:04:45.84 CSR.select :AqMeOH @13:04:45.95 CS.reconfigure :default @13:04:45.99 CS.seek 2ml @13:04:51.84 delay 5 seconds @13:04:56.86 CSR.select :air @13:04:57.01 CS.seek 5ml @13:05:05.34 delay 2 seconds @13:05:07.36 CSV.dial CTV @13:05:07.79 CS.seek 1ml @13:05:18.79 Before lysing Collection Puck Heater at 11.7C/38.3psia @13:05:18.81 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @13:05:18.83 CH.seek 70C,8:00 @13:07:31.29 CH.hold 10:00,70C @13:07:31.47 Begun lysing Collection Puck Heater at 68C/42.5psia @13:17:31.48 Ended lysing Collection Puck Heater at 70C/44.2psia @13:17:31.49 CS.seek 0ml @13:17:34.80 CTV.dial between spare and air,avoiding: :intake @13:17:35.79 CSV.dial :air @13:17:36.24 CS.seek 5ml @13:17:49.63 CSV.dial CTV @13:17:50.07 delay 2 seconds @13:17:52.09 CSV.dial :air @13:17:52.54 delay 2 seconds @13:17:54.56 CSV.dial CTV @13:17:55.02 delay 2 seconds @13:17:57.03 CSV.dial :air @13:17:57.48 delay 2 seconds @13:17:59.50 CS.seek 0ml @13:18:13.03 Puck @71.2psia after final lysis pressurization @13:18:13.04 Recovering Lysate into Collection syringe @13:18:13.05 CSV.dial CBV @13:18:14.18 CBV.dial CSV @13:18:14.54 CS.seek 5ml @13:18:27.92 delay 5 seconds @13:18:32.93 CTV.dial :air @13:18:33.44 CS.seek 7ml @13:18:39.29 delay 5 seconds @13:18:44.31 CSV.dial :air2 @13:18:44.80 CS.seek 10ml @13:18:53.23 CSV.dial between air and CTV,avoiding: CBV @13:18:54.37 Lysing Complete @13:18:54.44 Storing sh1 puck in Collection Clamp to tube 2 @13:18:54.60 SC.reconfigure :tight @13:18:54.65 SC.seek 2 @13:18:58.15 SC.configure :limp @13:18:58.35 Elbow.reconfigure :tight @13:18:58.39 Elbow.seek 2 @13:19:00.19 Forearm.reconfigure :tight @13:19:00.23 Forearm.seek 2 + 700 counts @13:19:01.28 SE.reconfigure :pick @13:19:01.33 SE.seek :beamBreak @13:19:02.72 SE.configure :default @13:19:02.86 Elbow.reconfigure :limp @13:19:02.90 Forearm.reconfigure :loose @13:19:02.94 Forearm.seek 2 @13:19:03.35 Elbow.hold @13:19:03.41 Elbow.reconfigure :tight @13:19:03.50 SE.seek 26.36 Pucks below Top Plate @13:19:04.00 Forearm.force -20 @13:19:04.03 Forearm.seek 2 + 306 counts @13:19:04.37 Forearm.reconfigure :tight @13:19:04.41 Forearm.seek 2 + 700 counts @13:19:04.69 SE.seek 27.36 Pucks below Top Plate @13:19:05.33 Forearm.reconfigure :loose @13:19:05.37 Forearm.seek :retract @13:19:06.30 Elbow.seek CC @13:19:07.91 Elbow.reconfigure :loose @13:19:08.00 Forearm.seek CC @13:19:08.96 Hand.close @13:19:09.68 CC.seek :open @13:19:14.10 Forearm.seek :retract @13:19:15.16 Elbow.reconfigure :tight @13:19:15.20 Elbow.seek 2 @13:19:17.40 Elbow.reconfigure :limp @13:19:17.44 Forearm.seek 2 @13:19:18.56 Elbow.hold @13:19:18.62 Elbow.reconfigure :tight @13:19:18.67 Hand.open @13:19:19.54 Forearm.force -20 @13:19:19.57 Forearm.seek 2 + 311 counts @13:19:19.88 Forearm.reconfigure :tight @13:19:19.93 Forearm.seek 2 + 700 counts @13:19:20.30 SE.seek :down @13:19:21.15 SE.stop @13:19:21.43
Forearm.reconfigure :loose @13:19:21.47 Forearm.seek :retract @13:19:22.36 Tranferring DA @13:19:22.37 Beginning samplePassC @13:19:22.39 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:19:22.57 Elbow.seek FlushPuck::Garage @13:19:24.42 Elbow.reconfigure :loose @13:19:24.51 Forearm.seek :garage @13:19:25.64 Hand.close @13:19:26.53 Forearm.seek :retract @13:19:27.61 Elbow.reconfigure :tight @13:19:27.65 Elbow.seek CC @13:19:30.59 Elbow.reconfigure :loose @13:19:30.67 Forearm.reconfigure :tight @13:19:30.72 Forearm.seek CC @13:19:32.01 CC.seek :closed @13:19:36.24 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.85 @13:19:36.32 Hand.open @13:19:37.11 Forearm.reconfigure :loose @13:19:37.15 Forearm.seek :retract @13:19:38.16 PSV.dial PSR @13:19:39.51 Pulling 1.5ml of PSR.flush @13:19:39.52 PSR.select :flush @13:19:39.63 PS.seek 1.5ml @13:19:55.75 delay 5 seconds @13:20:00.77 PSV.dial :waste1 @13:20:01.21 PS.seek 0ml @13:20:17.28 PSV.dial PSR @13:20:17.72 PSR.select :air @13:20:17.83 PS.seek 2.5ml @13:20:44.00 PRV.dial CBV,avoiding: :ab2 @13:20:44.02
CBV.dial PRV @13:20:45.69 PSV.dial :mixloop @13:20:46.69 CTV.dial :air,avoiding: :intake @13:20:46.70 PTV.dial :PRVmixing @13:20:47.95 PS.seek 0ml @13:21:14.18 PSV.dial :air @13:21:14.63 PS.seek 2.5ml @13:21:40.84 PSV.dial :mixloop @13:21:41.28 PS.seek 0ml @13:22:07.46 PSV.dial :air @13:22:07.91 PS.seek 2.5ml @13:22:34.09 PSV.dial :mixloop @13:22:34.53 PS.seek 0ml @13:23:00.78 Garaging FlushPuck in Collection Clamp @13:23:00.85 Elbow.reconfigure :tight @13:23:00.89 Elbow.seek CC @13:23:01.11 Elbow.reconfigure :loose @13:23:01.20 Forearm.seek CC @13:23:02.24 Hand.close @13:23:02.92 CC.seek :open @13:23:07.24 Forearm.seek :retract @13:23:08.26 Elbow.reconfigure :tight @13:23:08.30 Elbow.seek :garage @13:23:11.11 Elbow.reconfigure :loose @13:23:11.67 Forearm.reconfigure :tight @13:23:11.71 Forearm.seek :garage @13:23:12.76 Hand.open @13:23:13.60 Forearm.reconfigure :loose @13:23:13.64 Forearm.seek :retract @13:23:14.70 PSV.dial :mixloop @13:23:14.71 PTV.dial :PRVmixing @13:23:14.72 PRV.dial CBV @13:23:14.75
CBV.dial PRV @13:23:14.76 CTV.dial :air,avoiding: :intake @13:23:14.78 Loading lyfil puck from tube 3 into Collection Clamp @13:23:15.00 SC.reconfigure :tight @13:23:15.04 SC.seek 3 @13:23:18.58 SC.configure :limp @13:23:18.78 Elbow.reconfigure :tight @13:23:18.82 Elbow.seek 3 @13:23:20.81 Forearm.reconfigure :tight @13:23:20.89 Forearm.seek 3 + 700 counts @13:23:21.96 SE.reconfigure :pick @13:23:22.01 SE.seek :beamBreak @13:23:24.82 SE.configure :default @13:23:24.96 Elbow.seek 3 @13:23:25.36 Forearm.seek 3 + 300 counts @13:23:25.59 Forearm.reconfigure :loose @13:23:25.63 Hand.close @13:23:26.30 SE.seek :down @13:23:26.49
Forearm.seek :retract @13:23:27.47 Elbow.seek CC @13:23:28.86 SE.stop @13:23:29.03
Elbow.reconfigure :loose @13:23:29.12 Forearm.reconfigure :tight @13:23:29.17 Forearm.seek CC @13:23:30.46 CC.seek :closed @13:23:34.68 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.63 @13:23:34.69 Hand.open @13:23:35.48 Forearm.reconfigure :loose @13:23:35.52 Forearm.seek :retract @13:23:36.53 CTV.dial CSV,avoiding: :intake @13:23:37.81 CSV.dial CTV @13:23:38.12 PS.seek 2.5ml @13:24:04.36 CS.seek 5.5ml @13:24:16.59 delay 8 seconds @13:24:24.62 PSV.dial :air @13:24:25.06 delay 2 seconds @13:24:27.07 PSV.dial :waste2 @13:24:27.92 PS.seek 0ml @13:24:54.08 CBV.dial between PRV and waste1 @13:24:54.41 CSV.dial :air @13:24:54.86 CS.seek 10ml @13:25:07.09 CSV.dial CTV @13:25:07.54 CS.seek 0ml @13:25:33.75 CBV.dial :waste1 @13:25:34.05 delay 5 seconds @13:25:39.07 CTV.dial :air,avoiding: :intake @13:25:40.41 Storing lyfil puck in Collection Clamp to tube 2 @13:25:40.58 SC.reconfigure :tight @13:25:40.62 SC.seek 2 @13:25:44.19 SC.configure :limp @13:25:44.39 Elbow.reconfigure :tight @13:25:44.43 Elbow.seek 2 @13:25:46.24 Forearm.reconfigure :tight @13:25:46.28 Forearm.seek 2 + 700 counts @13:25:47.33 SE.reconfigure :pick @13:25:47.85 SE.seek :beamBreak @13:25:49.13 SE.configure :default @13:25:49.28 Elbow.reconfigure :limp @13:25:49.33 Forearm.reconfigure :loose @13:25:49.37 Forearm.seek 2 @13:25:49.75 Elbow.hold @13:25:49.81 Elbow.reconfigure :tight @13:25:49.91 SE.seek 27.36 Pucks below Top Plate @13:25:50.34 Forearm.force -20 @13:25:50.38 Forearm.seek 2 + 318 counts @13:25:50.65 Forearm.reconfigure :tight @13:25:50.70 Forearm.seek 2 + 700 counts @13:25:51.00 SE.seek 28.36 Pucks below Top Plate @13:25:51.57 Forearm.reconfigure :loose @13:25:51.62 Forearm.seek :retract @13:25:52.51 Elbow.seek CC @13:25:54.04 Elbow.reconfigure :loose @13:25:54.13 Forearm.seek CC @13:25:55.11 Hand.close @13:25:55.79 CC.seek :open @13:26:00.22 Forearm.seek :retract @13:26:01.24 Elbow.reconfigure :tight @13:26:01.28 Elbow.seek 2 @13:26:02.99 Elbow.reconfigure :limp @13:26:03.03 Forearm.seek 2 @13:26:04.10 Elbow.hold @13:26:04.16 Elbow.reconfigure :tight @13:26:04.20 Hand.open @13:26:05.03 Forearm.force -20 @13:26:05.06 Forearm.seek 2 + 314 counts @13:26:05.39 Forearm.reconfigure :tight @13:26:05.44 Forearm.seek 2 + 700 counts @13:26:05.73 SE.seek :down @13:26:06.58 SE.stop @13:26:06.85
Forearm.reconfigure :loose @13:26:06.90 Forearm.seek :retract @13:26:07.83 PSV.dial :air @13:26:08.54 PS.seek 2.5ml @13:26:34.74 delay 1 second @13:26:35.75 PSV.dial :mixloop @13:26:36.18 delay 1 second @13:26:37.19 PSV.dial :air @13:26:37.63 delay 1 second @13:26:38.64 PSV.dial :mixloop @13:26:39.08 delay 1 second @13:26:40.10 PSV.dial :air @13:26:40.54 delay 1 second @13:26:41.56 PS.seek 0ml @13:27:07.71 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:27:07.88 Elbow.seek FlushPuck::Garage @13:27:09.70 Elbow.reconfigure :loose @13:27:09.78 Forearm.seek :garage @13:27:10.91 Hand.close @13:27:11.65 Forearm.seek :retract @13:27:12.76 Elbow.reconfigure :tight @13:27:12.80 Elbow.seek PC @13:27:14.12 Elbow.reconfigure :loose @13:27:14.21 Forearm.reconfigure :tight @13:27:14.26 Forearm.seek PC @13:27:15.64 PC.seek :closed @13:27:19.53 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.36 @13:27:19.61 Hand.open @13:27:20.84 Forearm.reconfigure :loose @13:27:20.88 Forearm.seek :retract @13:27:21.91 PRV.dial :air @13:27:22.35 PSV.dial :mixloop @13:27:22.87 PS.seek 0.3ml @13:27:26.77 PSV.dial PSR @13:27:27.82 Pulling 0.4ml of PSR.air @13:27:27.83 PSR.select :air @13:27:27.86 PS.seek 0.7ml @13:27:32.77 delay 3 seconds @13:27:35.78 PSR.select :air @13:27:35.81 PS.seek 2.5ml @13:27:54.86 delay 3 seconds @13:27:57.88 PSV.dial :mixloop @13:27:58.88 PTV.dial :mixing @13:27:59.60 PBV.dial :waste2 @13:28:00.51 PS.seek 0ml @13:28:26.71 Garaging FlushPuck in Processing Clamp @13:28:26.78 Elbow.reconfigure :tight @13:28:26.82 Elbow.seek PC @13:28:26.93 Elbow.reconfigure :loose @13:28:27.02 Forearm.seek PC @13:28:28.09 Hand.close @13:28:28.66 PC.seek :open @13:28:32.63 Forearm.seek :retract @13:28:33.66 Elbow.reconfigure :tight @13:28:33.70 Elbow.seek :garage @13:28:35.07 Elbow.reconfigure :loose @13:28:35.16 Forearm.reconfigure :tight @13:28:35.20 Forearm.seek :garage @13:28:36.34 Hand.open @13:28:37.09 Forearm.reconfigure :loose @13:28:37.13 Forearm.seek :retract @13:28:38.19 Loading da puck from tube 3 into Processing Clamp @13:28:38.41 SC.reconfigure :tight @13:28:38.45 SC.seek 3 @13:28:42.03 SC.configure :limp @13:28:42.22 Elbow.reconfigure :tight @13:28:42.26 Elbow.seek 3 @13:28:44.15 Forearm.reconfigure :tight @13:28:44.19 Forearm.seek 3 + 700 counts @13:28:45.21 SE.reconfigure :pick @13:28:45.26 SE.seek :beamBreak @13:28:48.12 SE.configure :default @13:28:48.27 Elbow.seek 3 @13:28:48.44 Forearm.seek 3 + 300 counts @13:28:48.68 Forearm.reconfigure :loose @13:28:48.73 Hand.close @13:28:49.30 SE.seek :down @13:28:49.50
Forearm.seek :retract @13:28:50.47 Elbow.seek PC @13:28:51.89 SE.stop @13:28:53.07
Elbow.reconfigure :loose @13:28:53.16 Forearm.reconfigure :tight @13:28:53.20 Forearm.seek PC @13:28:54.59 PC.seek :closed @13:28:58.45 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.35 @13:28:58.46 Hand.open @13:28:59.21 Forearm.reconfigure :loose @13:28:59.25 Forearm.seek :retract @13:29:00.27 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @13:29:00.29 PH.seek 28C,5:00 @13:29:49.57 PH.hold 2:00:00,28C @13:29:49.63 delay 1:30 @13:31:19.65 PSR.select :air @13:31:19.67 PSV.dial PSR @13:31:20.74 Pulling 0.5ml of PSR.wash @13:31:20.75 PSR.select :wash @13:31:20.86 PS.seek 0.5ml @13:31:26.70 delay 5 seconds @13:31:31.72 PSR.select :air @13:31:31.84 PS.seek 2ml @13:31:47.82 PTV.dial :puck @13:31:48.51 PSV.dial PTV @13:31:49.25 PBV.dial :waste1 @13:31:50.59 PS.seek 1.5ml @13:31:56.45 delay 3 seconds @13:31:59.47 PBV.dial between PSV and waste1 @13:31:59.83 PS.seek 0ml @13:32:15.86 delay 5:00 @13:37:15.88 recoverPSRreagent08 @13:37:15.89 PTV.dial :puck @13:37:15.90 PBV.dial :waste1 @13:37:16.25 delay 30 seconds @13:37:46.28 PBV.dial between PSV and waste1 @13:37:46.63 PSR.select :air @13:37:46.65 PSV.dial PSR @13:37:47.38 PS.reconfigure :fast @13:37:47.43 PS.seek 2.5ml @13:38:10.66 PSV.dial PTV @13:38:11.38 delay 5 seconds @13:38:16.40 PSV.dial PSR @13:38:17.13 delay 5 seconds @13:38:22.15 PSV.dial PTV @13:38:22.88 delay 5 seconds @13:38:27.90 PSV.dial :air @13:38:28.62 PS.seek 0ml @13:38:51.35 PTV.dial :PRVmixing,via: PRV @13:38:52.61 PSV.dial :mixloop @13:38:53.06 PRV.dial :ab1 @13:38:53.82 PS.reconfigure :slow @13:38:53.86 PS.seek 0.15ml @13:38:57.44 delay 2 seconds @13:38:59.46 CBV.dial PRV @13:38:59.48
PRV.dial CBV @13:39:00.04 PS.seek 0.34ml @13:39:04.38 delay 2 seconds @13:39:06.39 CBV.dial between PRV and PSV @13:39:06.70 PRV.dial :air @13:39:07.15 PS.seek 0.9ml @13:39:19.06 PRV.dial :waste2 @13:39:19.79 PS.seek 0.5ml @13:39:28.38 addPMLReagent08 @13:39:28.39 PSV.dial :air @13:39:28.83 PS.reconfigure :default @13:39:28.88 PS.seek 2ml @13:39:44.90 PSV.dial :PML @13:39:45.34 PTV.dial :mixing @13:39:46.06 PBV.dial :waste1 @13:39:46.47 PS.seek 1.85ml @13:39:48.81 PBV.dial between PSV and waste1 @13:39:49.20 PS.seek 0ml @13:40:08.78 delay 2 seconds @13:40:10.80 Beginning samplePassC @13:40:10.81 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:40:10.97 Elbow.reconfigure :tight @13:40:11.02 Elbow.seek FlushPuck::Garage @13:40:12.39 Elbow.reconfigure :loose @13:40:12.48 Forearm.seek :garage @13:40:13.62 Hand.close @13:40:14.37 Forearm.seek :retract @13:40:15.43 Elbow.reconfigure :tight @13:40:15.48 Elbow.seek CC @13:40:18.23 Elbow.reconfigure :loose @13:40:18.33 Forearm.reconfigure :tight @13:40:18.37 Forearm.seek CC @13:40:19.68 CC.seek :closed @13:40:23.91 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.80 @13:40:23.97 Hand.open @13:40:24.83 Forearm.reconfigure :loose @13:40:24.87 Forearm.seek :retract @13:40:25.91 PSV.dial PSR @13:40:26.96 Pulling 1.5ml of PSR.flush @13:40:26.97 PSR.select :flush @13:40:27.09 PS.seek 1.5ml @13:40:43.06 delay 5 seconds @13:40:48.08 PSV.dial :waste1 @13:40:48.54 PS.seek 0ml @13:41:04.62 PSV.dial PSR @13:41:05.08 PSR.select :air @13:41:05.19 PS.seek 2.5ml @13:41:31.41 PRV.dial CBV,avoiding: :ab2 @13:41:31.43
CBV.dial PRV @13:41:33.01 PSV.dial :mixloop @13:41:34.03 CTV.dial :air,avoiding: :intake @13:41:34.04 PTV.dial :PRVmixing @13:41:34.75 PS.seek 0ml @13:42:00.93 PSV.dial :air @13:42:01.36 PS.seek 2.5ml @13:42:27.53 PSV.dial :mixloop @13:42:27.97 PS.seek 0ml @13:42:54.15 PSV.dial :air @13:42:54.60 PS.seek 2.5ml @13:43:20.74 PSV.dial :mixloop @13:43:21.19 PS.seek 0ml @13:43:47.31 Garaging FlushPuck in Collection Clamp @13:43:47.38 Elbow.reconfigure :tight @13:43:47.42 Elbow.seek CC @13:43:47.52 Elbow.reconfigure :loose @13:43:47.61 Forearm.seek CC @13:43:48.59 Hand.close @13:43:49.38 CC.seek :open @13:43:53.74 Forearm.seek :retract @13:43:54.71 Elbow.reconfigure :tight @13:43:54.75 Elbow.seek :garage @13:43:57.60 Elbow.reconfigure :loose @13:43:57.69 Forearm.reconfigure :tight @13:43:57.73 Forearm.seek :garage @13:43:58.87 Hand.open @13:43:59.69 Forearm.reconfigure :loose @13:43:59.73 Forearm.seek :retract @13:44:00.75 delayUntil "13:48:10.79" @13:44:00.78 Whole Cell Archive sampling 1000ml @13:44:00.79 Loading wcr puck from tube 3 into Collection Clamp @13:44:01.24 Elbow.reconfigure :tight @13:44:01.29 Elbow.seek 3 @13:44:03.27 Forearm.reconfigure :tight @13:44:03.32 Forearm.seek 3 + 700 counts @13:44:04.33 SE.reconfigure :pick @13:44:04.39 SE.seek :beamBreak @13:44:07.42 SE.configure :default @13:44:07.57 Elbow.seek 3 @13:44:07.74 Forearm.seek 3 + 300 counts @13:44:08.06 Forearm.reconfigure :loose @13:44:08.11 Hand.close @13:44:08.89 SE.seek :down @13:44:09.08 Forearm.seek :retract @13:44:10.10 Elbow.seek CC @13:44:11.60 SE.stop @13:44:11.66 Elbow.reconfigure :loose @13:44:11.75 Forearm.reconfigure :tight @13:44:11.80 Forearm.seek CC @13:44:13.57 CC.seek :closed @13:44:17.81 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.69 @13:44:17.82 Hand.open @13:44:18.56 Forearm.reconfigure :loose @13:44:18.61 Forearm.seek :retract @13:44:19.61 collectSample 1000ml w/bubble point of 35psi @13:44:19.63 Priming 7ml @13:44:19.64 Intake.select :close @13:44:19.65 Exhaust.select :close @13:44:19.66 SS.hold @13:44:19.85 Calibrating pressure sensors @13:44:19.86 CTV.dial :air @13:44:19.88 SSV.dial :spit,avoiding: :exhaust @13:44:19.89 SS.reconfigure :fast @13:44:19.94 SS.seek 5ml @13:44:22.73 SSV.dial CBV @13:44:22.75 CBV.dial SSV @13:44:24.09 delay 3.8 seconds @13:44:28.00 SSV.dial :spit @13:44:29.33 SS.seek 0ml @13:44:31.96 SSV.dial CBV @13:44:33.27 CTV.dial :intake @13:44:34.00 SSV.dial CBV @13:44:34.03 CBV.dial SSV @13:44:34.05 Allowing 40s for 7.0ml of remaining 7.0ml @13:44:34.06 Intake.select :open @13:44:36.21 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:44:36.23 SS.reconfigure :inhaling 2.14ml/s @13:44:36.27 SS.seek 7ml @13:44:42.77 Stroke completed -- waiting for pressure to equalize ... @13:44:42.78 SS.reconfigure :exhale @13:44:42.82 SS.hold @13:45:01.22 Intake.select :close @13:45:01.36 SSV.dial :exhaust @13:45:02.11 Exhaust.select :open @13:45:02.23 SS.seek 0ml @13:45:06.44 Exhaust.select :close @13:45:06.55 Sampling 1000.0ml @13:45:06.56 Intake.select :close @13:45:06.57 Exhaust.select :close @13:45:06.58 SS.reconfigure :default @13:45:06.62 SS.hold @13:45:06.82 CTV.dial :intake @13:45:06.83 SSV.dial CBV @13:45:06.87 CBV.dial SSV @13:45:07.61 Allowing 130s for 25.0ml of remaining 1000.0ml @13:45:07.62 Intake.select :open @13:45:09.77 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:45:09.79 SS.reconfigure :inhaling 2.14ml/s @13:45:09.83 SS.seek 25ml @13:45:15.00 Vacuum too high -- waiting up to 40 seconds @13:45:15.01 SS.reconfigure :exhale @13:45:15.05 SS.hold @13:45:20.21 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:45:20.70 SS.reconfigure :inhaling 1.93ml/s @13:45:20.76 SS.seek 25ml @13:45:23.71 Vacuum too high -- waiting up to 40 seconds @13:45:23.73 SS.reconfigure :exhale @13:45:23.77 SS.hold @13:45:27.00 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:45:27.02 SS.reconfigure :inhaling 1.74ml/s @13:45:27.07 SS.seek 25ml @13:45:29.34 Vacuum too high -- waiting up to 40 seconds @13:45:29.35 SS.reconfigure :exhale @13:45:29.40 SS.hold @13:45:32.07 Intake @16.0psia, vacuum from 10.0 to 13.0psi @13:45:32.09 SS.reconfigure :inhaling 1.57ml/s @13:45:32.13 SS.seek 25ml @13:45:34.43 Vacuum too high -- waiting up to 40 seconds @13:45:34.45 SS.reconfigure :exhale @13:45:34.49 SS.hold @13:45:36.70 SS.reconfigure :inhaling 1.41ml/s @13:45:36.74 SS.seek 25ml @13:45:39.49 Vacuum too high -- waiting up to 40 seconds @13:45:39.51 SS.reconfigure :exhale @13:45:39.55 SS.hold @13:45:41.78 Intake @15.9psia, vacuum from 10.0 to 13.0psi @13:45:41.80 SS.reconfigure :inhaling 1.27ml/s @13:45:41.84 SS.seek 25ml @13:45:43.93 Vacuum too high -- waiting up to 40 seconds @13:45:43.95 SS.reconfigure :exhale @13:45:43.99 SS.hold @13:45:46.08 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:45:46.10 SS.reconfigure :inhaling 1.14ml/s @13:45:46.15 SS.seek 25ml @13:45:49.06 Vacuum too high -- waiting up to 40 seconds @13:45:49.07 SS.reconfigure :exhale @13:45:49.11 SS.hold @13:45:51.19 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:45:51.21 SS.reconfigure :inhaling 1.03ml/s @13:45:51.25 SS.seek 25ml @13:45:54.18 Vacuum too high -- waiting up to 40 seconds @13:45:54.20 SS.reconfigure :exhale @13:45:54.24 SS.hold @13:45:56.75 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:45:56.77 SS.reconfigure :inhaling 0.93ml/s @13:45:56.81 SS.seek 25ml @13:45:59.93 Vacuum too high -- waiting up to 40 seconds @13:45:59.94 SS.reconfigure :exhale @13:45:59.99 SS.hold @13:46:02.50 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:46:02.52 SS.reconfigure :inhaling 0.84ml/s @13:46:02.56 SS.seek 25ml @13:46:05.27 Stroke completed -- waiting for pressure to equalize ... @13:46:05.28 SS.reconfigure :exhale @13:46:05.32 SS.hold @13:46:08.73 Intake.select :close @13:46:08.87 SSV.dial :exhaust @13:46:09.61 Exhaust.select :open @13:46:09.72 SS.seek 0ml @13:46:18.81 Exhaust.select :close @13:46:18.92 SSV.dial CBV @13:46:18.94 CBV.dial SSV @13:46:19.72 Allowing 130s for 25.0ml of remaining 975.0ml @13:46:19.74 Intake.select :open @13:46:21.89 Intake @15.3psia, vacuum from 9.8 to 12.8psi @13:46:21.91 SS.reconfigure :inhaling 0.84ml/s @13:46:21.95 SS.seek 25ml @13:46:26.77 Vacuum too high -- waiting up to 40 seconds @13:46:26.79 SS.reconfigure :exhale @13:46:26.83 SS.hold @13:46:28.48 Intake @15.8psia, vacuum from 10.0 to 13.0psi @13:46:28.50 SS.reconfigure :inhaling 0.76ml/s @13:46:28.54 SS.seek 25ml @13:46:32.09 Vacuum too high -- waiting up to 40 seconds @13:46:32.10 SS.reconfigure :exhale @13:46:32.14 SS.hold @13:46:33.79 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:46:33.81 SS.reconfigure :inhaling 0.69ml/s @13:46:33.85 SS.seek 25ml @13:46:38.68 Vacuum too high -- waiting up to 40 seconds @13:46:38.69 SS.reconfigure :exhale @13:46:38.73 SS.hold @13:46:40.40 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:46:40.42 SS.reconfigure :inhaling 0.61ml/s @13:46:40.46 SS.seek 25ml @13:46:47.65 Vacuum too high -- waiting up to 40 seconds @13:46:47.66 SS.reconfigure :exhale @13:46:47.70 SS.hold @13:46:49.76 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:46:49.78 SS.reconfigure :inhaling 0.56ml/s @13:46:49.82 SS.seek 25ml @13:46:56.90 Vacuum too high -- waiting up to 40 seconds @13:46:56.91 SS.reconfigure :exhale @13:46:56.95 SS.hold @13:46:58.60 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:46:58.62 SS.reconfigure :inhaling 0.50ml/s @13:46:58.66 SS.seek 25ml @13:47:14.93 Vacuum too high -- waiting up to 40 seconds @13:47:14.94 SS.reconfigure :exhale @13:47:14.98 SS.hold @13:47:17.37 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:47:17.39 SS.reconfigure :inhaling 0.46ml/s @13:47:17.43 SS.seek 25ml @13:47:22.06 Stroke completed -- waiting for pressure to equalize ... @13:47:22.07 SS.reconfigure :exhale @13:47:22.11 SS.hold @13:47:25.01 Intake.select :close @13:47:25.16 SSV.dial :exhaust @13:47:25.91 Exhaust.select :open @13:47:26.02 SS.seek 0ml @13:47:35.14 Exhaust.select :close @13:47:35.73 SSV.dial CBV @13:47:35.77 CBV.dial SSV @13:47:36.50 Allowing 130s for 25.0ml of remaining 950.0ml @13:47:36.52 Intake.select :open @13:47:38.68 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:47:38.70 SS.reconfigure :inhaling 0.46ml/s @13:47:38.74 SS.seek 25ml @13:48:10.79 recoverPRVreagent08 @13:48:10.81 PTV.dial :mixing @13:48:11.53 PBV.dial :waste1 @13:48:11.92 delay 30 seconds @13:48:25.77 Vacuum too high -- waiting up to 40 seconds @13:48:25.78 SS.reconfigure :exhale @13:48:25.83 SS.hold @13:48:28.33 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:48:28.35 SS.reconfigure :inhaling 0.41ml/s @13:48:28.39 SS.seek 25ml @13:48:41.23 Stroke completed -- waiting for pressure to equalize ... @13:48:41.24 SS.reconfigure :exhale @13:48:41.28 SS.hold @13:48:41.93 PBV.dial between PSV and waste1 @13:48:42.28 PSV.dial :air @13:48:42.74 PS.reconfigure :fast @13:48:42.79 PS.seek 2.5ml @13:48:45.51 Intake.select :close @13:48:45.66 SSV.dial :exhaust @13:48:46.40 Exhaust.select :open @13:48:46.51 SS.seek 0ml @13:48:55.62 Exhaust.select :close @13:48:55.73 SSV.dial CBV @13:48:55.75 CBV.dial SSV @13:48:56.53 Allowing 130s for 25.0ml of remaining 925.0ml @13:48:56.54 Intake.select :open @13:48:58.70 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:48:58.72 SS.reconfigure :inhaling 0.41ml/s @13:48:58.76 SS.seek 25ml @13:49:06.14 PSV.dial :mixloop @13:49:06.60 delay 2 seconds @13:49:08.61 PSV.dial :air @13:49:09.06 delay 2 seconds @13:49:11.08 PSV.dial :mixloop @13:49:11.54 delay 2 seconds @13:49:13.56 PSV.dial :air @13:49:14.01 PS.seek 0ml @13:49:36.86 washCycleDA #1 of 2 @13:49:36.87 PSR.select :air @13:49:36.89 PSV.dial PSR @13:49:38.35 Pulling 0.5ml of PSR.wash @13:49:38.36 PSR.select :wash @13:49:38.47 PS.reconfigure :default @13:49:38.52 PS.seek 0.5ml @13:49:44.52 delay 5 seconds @13:49:49.54 PSR.select :air @13:49:49.66 PS.seek 2ml @13:50:05.07 Stroke completed -- waiting for pressure to equalize ... @13:50:05.08 SS.reconfigure :exhale @13:50:05.13 SS.hold @13:50:05.70 PTV.dial :puck @13:50:06.39 PSV.dial PTV @13:50:07.14 PBV.dial :waste1 @13:50:07.56 PS.seek 1.5ml @13:50:11.53 Intake.select :close @13:50:11.68 SSV.dial :exhaust @13:50:12.42 Exhaust.select :open @13:50:12.53 SS.seek 0ml @13:50:13.42 delay 3 seconds @13:50:16.44 PBV.dial between PSV and waste1 @13:50:16.80 PS.seek 0ml @13:50:22.00 Exhaust.select :close @13:50:22.11 SSV.dial CBV @13:50:22.13 CBV.dial SSV @13:50:22.89 Allowing 130s for 25.0ml of remaining 900.0ml @13:50:22.90 Intake.select :open @13:50:25.05 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:50:25.53 SS.reconfigure :inhaling 0.41ml/s @13:50:25.59 SS.seek 25ml @13:50:32.89 delay 2:00 @13:51:30.82 Stroke completed -- waiting for pressure to equalize ... @13:51:30.83 SS.reconfigure :exhale @13:51:30.87 SS.hold @13:51:40.59 Intake.select :close @13:51:40.73 SSV.dial :exhaust @13:51:41.47 Exhaust.select :open @13:51:41.58 SS.seek 0ml @13:51:51.08 Exhaust.select :close @13:51:51.19 SSV.dial CBV @13:51:51.22 CBV.dial SSV @13:51:51.96 Allowing 130s for 25.0ml of remaining 875.0ml @13:51:51.98 Intake.select :open @13:51:54.13 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:51:54.15 SS.reconfigure :inhaling 0.41ml/s @13:51:54.19 SS.seek 25ml @13:52:29.33 Vacuum too high -- waiting up to 40 seconds @13:52:29.34 SS.reconfigure :exhale @13:52:29.38 SS.hold @13:52:32.91 recoverPSRreagent08 @13:52:32.92 PTV.dial :puck @13:52:32.93 PBV.dial :waste1 @13:52:33.29 delay 30 seconds @13:52:33.57 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:52:33.59 SS.reconfigure :inhaling 0.37ml/s @13:52:33.63 SS.seek 25ml @13:53:03.32 PBV.dial between PSV and waste1 @13:53:03.68 PSR.select :air @13:53:03.70 PSV.dial PSR @13:53:04.44 PS.reconfigure :fast @13:53:04.48 PS.seek 2.5ml @13:53:07.56 Stroke completed -- waiting for pressure to equalize ... @13:53:07.57 SS.reconfigure :exhale @13:53:07.61 SS.hold @13:53:17.30 Intake.select :close @13:53:17.44 SSV.dial :exhaust @13:53:18.20 Exhaust.select :open @13:53:18.31 SS.seek 0ml @13:53:27.76 PSV.dial PTV @13:53:27.82 Exhaust.select :close @13:53:27.93 SSV.dial CBV @13:53:27.95 CBV.dial SSV @13:53:28.50 delay 5 seconds @13:53:28.72 Allowing 130s for 25.0ml of remaining 850.0ml @13:53:28.74 Intake.select :open @13:53:30.90 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:53:30.92 SS.reconfigure :inhaling 0.37ml/s @13:53:30.96 SS.seek 25ml @13:53:33.51 PSV.dial PSR @13:53:34.25 delay 5 seconds @13:53:39.27 PSV.dial PTV @13:53:40.00 delay 5 seconds @13:53:45.01 PSV.dial :air @13:53:45.75 PS.seek 0ml @13:54:06.45 Vacuum too high -- waiting up to 40 seconds @13:54:06.46 SS.reconfigure :exhale @13:54:06.50 SS.hold @13:54:08.66 washCycleDA #2 of 2 @13:54:08.68 PSR.select :air @13:54:08.69 PSV.dial PSR @13:54:10.14 Pulling 0.5ml of PSR.wash @13:54:10.15 PSR.select :wash @13:54:10.26 PS.reconfigure :default @13:54:10.31 PS.seek 0.5ml @13:54:10.59 Intake @15.7psia, vacuum from 10.0 to 13.0psi @13:54:10.61 SS.reconfigure :inhaling 0.33ml/s @13:54:10.65 SS.seek 25ml @13:54:16.20 delay 5 seconds @13:54:21.22 PSR.select :air @13:54:21.33 PS.seek 2ml @13:54:37.32 PTV.dial :puck @13:54:37.33 PSV.dial PTV @13:54:38.07 PBV.dial :waste1 @13:54:38.51 PS.seek 1.5ml @13:54:44.35 delay 3 seconds @13:54:47.37 PBV.dial between PSV and waste1 @13:54:47.75 PS.seek 0ml @13:54:55.33 Stroke completed -- waiting for pressure to equalize ... @13:54:55.34 SS.reconfigure :exhale @13:54:55.38 SS.hold @13:55:03.95 delay 2:00 @13:55:06.45 Intake.select :close @13:55:06.59 SSV.dial :exhaust @13:55:07.34 Exhaust.select :open @13:55:07.46 SS.seek 0ml @13:55:17.06 Exhaust.select :close @13:55:17.17 SSV.dial CBV @13:55:17.20 CBV.dial SSV @13:55:17.94 Allowing 130s for 25.0ml of remaining 825.0ml @13:55:17.95 Intake.select :open @13:55:20.11 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:55:20.13 SS.reconfigure :inhaling 0.33ml/s @13:55:20.17 SS.seek 25ml @13:56:19.29 Vacuum too high -- waiting up to 40 seconds @13:56:19.30 SS.reconfigure :exhale @13:56:19.34 SS.hold @13:56:24.99 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:56:25.01 SS.reconfigure :inhaling 0.30ml/s @13:56:25.06 SS.seek 25ml @13:56:53.89 Stroke completed -- waiting for pressure to equalize ... @13:56:53.90 SS.reconfigure :exhale @13:56:53.94 SS.hold @13:57:03.97 recoverPSRreagent08 @13:57:03.98 PTV.dial :puck @13:57:03.99 PBV.dial :waste1 @13:57:04.35 delay 30 seconds @13:57:05.09 Intake.select :close @13:57:05.24 SSV.dial :exhaust @13:57:05.98 Exhaust.select :open @13:57:06.09 SS.seek 0ml @13:57:15.23 Exhaust.select :close @13:57:15.34 SSV.dial CBV @13:57:15.36 CBV.dial SSV @13:57:16.64 Allowing 130s for 25.0ml of remaining 800.0ml @13:57:16.65 Intake.select :open @13:57:18.81 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:57:18.83 SS.reconfigure :inhaling 0.30ml/s @13:57:18.87 SS.seek 25ml @13:57:34.37 PBV.dial between PSV and waste1 @13:57:34.74 PSR.select :air @13:57:34.76 PSV.dial PSR @13:57:35.49 PS.reconfigure :fast @13:57:35.54 PS.seek 2.5ml @13:57:58.78 PSV.dial PTV @13:57:59.52 delay 5 seconds @13:58:04.54 PSV.dial PSR @13:58:05.40 delay 5 seconds @13:58:10.41 PSV.dial PTV @13:58:11.13 delay 5 seconds @13:58:16.15 PSV.dial :air @13:58:16.89 PS.seek 0ml @13:58:18.85 Vacuum too high -- waiting up to 40 seconds @13:58:18.86 SS.reconfigure :exhale @13:58:18.90 SS.hold @13:58:25.43 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:58:25.45 SS.reconfigure :inhaling 0.27ml/s @13:58:25.49 SS.seek 25ml @13:58:39.72 getPMLReagent08 @13:58:39.73 PRV.dial :air @13:58:40.18 delay 5 seconds @13:58:45.19 PTV.dial :PRVmixing @13:58:46.46 delay 5 seconds @13:58:51.48 PRV.dial :ab2 @13:58:51.92 PTV.dial :PRVmixing @13:58:51.94 PSV.dial :mixloop @13:58:52.51 PS.reconfigure :default @13:58:52.55 PS.seek 0.3ml @13:58:56.47 delay 2 seconds @13:58:58.49 PRV.dial :air @13:58:59.00 PS.seek 0.55ml @13:59:02.38 addPMLReagent08 @13:59:02.39 PSV.dial :air @13:59:02.82 PS.seek 2ml @13:59:03.69 Stroke completed -- waiting for pressure to equalize ... @13:59:03.70 SS.reconfigure :exhale @13:59:03.74 SS.hold @13:59:18.37 PSV.dial :PML @13:59:18.81 PTV.dial :mixing @13:59:19.55 PBV.dial :waste1 @13:59:19.97 Intake.select :close @13:59:20.08 PS.seek 1.85ml @13:59:20.17 SSV.dial :exhaust @13:59:20.92 Exhaust.select :open @13:59:21.03 SS.seek 0ml @13:59:22.44 PBV.dial between PSV and waste1 @13:59:22.82 PS.seek 0ml @13:59:30.65 Exhaust.select :close @13:59:30.76 SSV.dial CBV @13:59:30.78 CBV.dial SSV @13:59:31.54 Allowing 130s for 25.0ml of remaining 775.0ml @13:59:31.55 Intake.select :open @13:59:33.71 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:59:33.73 SS.reconfigure :inhaling 0.27ml/s @13:59:33.77 SS.seek 25ml @13:59:42.47 delay 2 seconds @13:59:44.49 delay 8:00 @14:00:40.06 Vacuum too high -- waiting up to 40 seconds @14:00:40.08 SS.reconfigure :exhale @14:00:40.12 SS.hold @14:00:47.77 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:00:47.79 SS.reconfigure :inhaling 0.24ml/s @14:00:47.83 SS.seek 25ml @14:01:31.39 Stroke completed -- waiting for pressure to equalize ... @14:01:31.41 SS.reconfigure :exhale @14:01:31.45 SS.hold @14:01:41.56 SS.reconfigure :default @14:01:41.61 SS.hold @14:01:41.72 Intake.select :close @14:01:41.87 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 250.0ml @14:01:41.88 SSV.dial :exhaust @14:01:42.63 Exhaust.select :open @14:01:42.74 SS.reconfigure :exhale @14:01:42.78 SS.seek 0ml @14:01:52.46 Exhaust.select :close @14:01:52.57 Sampled 250.0ml @14:01:52.59 Drying puck before fixing for 20:00 CBV.dial :waste2b @14:01:53.32 delay 1:00 @14:02:53.35 CSV.dial :air @14:02:53.81 CS.reconfigure :fast @14:02:53.85 CS.seek 10ml @14:03:17.22 Pumping wcr puck from tube 3 up to 35psi... @14:03:17.24 CBV.dial SSV @14:03:18.54 CBV.dial between CSV and waste2b @14:03:19.47 CSV.dial between air and CTV @14:03:19.79 CS.seek 7.39ml @14:03:26.48 CTV.dial CSV,avoiding: :air @14:03:26.50 CSV.dial CTV @14:03:27.27 CS.reconfigure :pressurize @14:03:27.32 CS.seek 0ml @14:03:40.99 CBV.dial :waste2b @14:03:41.32 CS.reconfigure :default @14:03:41.37 CS.seek 0ml @14:03:47.56 delay 1:00 @14:04:47.59 CTV.dial :air,avoiding: :intake @14:04:48.90 Fix #1 of 2 (with RNAlater) for 10:00 @14:04:48.91 CSV.dial CSR @14:04:49.40 Pulling 2ml of CSR.RNAlater @14:04:49.41 CSR.select :RNAlater @14:04:49.52 CS.seek 2ml @14:04:55.35 delay 5 seconds @14:05:00.37 CSR.select :air @14:05:00.48 CS.reconfigure :fast @14:05:00.53 CS.seek 10ml @14:05:19.13 Pumping wcr puck from tube 3 up to 35psi... @14:05:19.14 CBV.dial SSV @14:05:20.43 CBV.dial between CSV and waste2b @14:05:21.36 CSV.dial between air and CTV @14:05:21.97 CS.seek 7.256ml @14:05:28.91 CTV.dial CSV,avoiding: :air @14:05:28.94 CSV.dial CTV @14:05:30.27 CS.reconfigure :pressurize @14:05:30.32 CS.seek 0ml @14:05:47.18 delay 10:00 @14:07:44.51 recoverPRVreagent08 @14:07:44.52 PTV.dial :mixing @14:07:44.53 PBV.dial :waste1 @14:07:44.90 delay 30 seconds @14:08:14.92 PBV.dial between PSV and waste1 @14:08:15.79 PSV.dial :air @14:08:16.24 PS.reconfigure :fast @14:08:16.29 PS.seek 2.5ml @14:08:39.21 PSV.dial :mixloop @14:08:39.66 delay 2 seconds @14:08:41.68 PSV.dial :air @14:08:42.13 delay 2 seconds @14:08:44.14 PSV.dial :mixloop @14:08:44.60 delay 2 seconds @14:08:46.62 PSV.dial :air @14:08:47.07 PS.seek 0ml @14:09:09.77 washCycleDA #1 of 3 @14:09:09.78 PSR.select :air @14:09:09.79 PSV.dial PSR @14:09:11.13 Pulling 0.5ml of PSR.wash @14:09:11.14 PSR.select :wash @14:09:11.26 PS.reconfigure :default @14:09:11.30 PS.seek 0.5ml @14:09:17.14 delay 5 seconds @14:09:22.16 PSR.select :air @14:09:22.29 PS.seek 2ml @14:09:38.26 PTV.dial :puck @14:09:38.99 PSV.dial PTV @14:09:39.72 PBV.dial :waste1 @14:09:40.15 PS.seek 1.5ml @14:09:46.05 delay 3 seconds @14:09:49.07 PBV.dial between PSV and waste1 @14:09:49.45 PS.seek 0ml @14:10:05.49 delay 2:00 @14:12:05.51 recoverPSRreagent08 @14:12:05.52 PTV.dial :puck @14:12:05.53 PBV.dial :waste1 @14:12:05.90 delay 30 seconds @14:12:35.93 PBV.dial between PSV and waste1 @14:12:36.29 PSR.select :air @14:12:36.31 PSV.dial PSR @14:12:37.05 PS.reconfigure :fast @14:12:37.09 PS.seek 2.5ml @14:13:00.32 PSV.dial PTV @14:13:01.06 delay 5 seconds @14:13:06.08 PSV.dial PSR @14:13:06.81 delay 5 seconds @14:13:11.83 PSV.dial PTV @14:13:12.56 delay 5 seconds @14:13:17.58 PSV.dial :air @14:13:18.30 PS.seek 0ml @14:13:41.20 washCycleDA #2 of 3 @14:13:41.21 PSR.select :air @14:13:41.23 PSV.dial PSR @14:13:42.55 Pulling 0.5ml of PSR.wash @14:13:42.56 PSR.select :wash @14:13:42.67 PS.reconfigure :default @14:13:42.71 PS.seek 0.5ml @14:13:48.61 delay 5 seconds @14:13:53.62 PSR.select :air @14:13:53.74 PS.seek 2ml @14:14:09.70 PTV.dial :puck @14:14:09.71 PSV.dial PTV @14:14:10.43 PBV.dial :waste1 @14:14:10.87 PS.seek 1.5ml @14:14:16.76 delay 3 seconds @14:14:19.78 PBV.dial between PSV and waste1 @14:14:20.14 PS.seek 0ml @14:14:36.17 delay 2:00 @14:15:47.19 CBV.dial :waste2b @14:15:47.52 CS.reconfigure :default @14:15:47.57 CS.seek 0ml @14:15:50.23 delay 1:00 @14:16:36.19 recoverPSRreagent08 @14:16:36.20 PTV.dial :puck @14:16:36.21 PBV.dial :waste1 @14:16:36.58 delay 30 seconds @14:16:50.26 CTV.dial :air,avoiding: :intake @14:16:51.55 Fix #2 of 2 (with RNAlater) for 10:00 @14:16:51.56 CSV.dial CSR @14:16:52.05 Pulling 2ml of CSR.RNAlater @14:16:52.06 CSR.select :RNAlater @14:16:52.18 CS.seek 2ml @14:16:57.98 delay 5 seconds @14:17:03.01 CSR.select :air @14:17:03.12 CS.reconfigure :fast @14:17:03.16 CS.seek 10ml @14:17:06.60 PBV.dial between PSV and waste1 @14:17:06.96 PSR.select :air @14:17:06.98 PSV.dial PSR @14:17:07.71 PS.reconfigure :fast @14:17:07.76 PS.seek 2.5ml @14:17:22.02 Pumping wcr puck from tube 3 up to 35psi... @14:17:22.03 CBV.dial SSV @14:17:23.31 CBV.dial between CSV and waste2b @14:17:24.24 CSV.dial between air and CTV @14:17:24.85 CS.seek 7.113ml @14:17:30.79 PSV.dial PTV @14:17:31.52 delay 5 seconds @14:17:32.08 CTV.dial CSV,avoiding: :air @14:17:32.10 CSV.dial CTV @14:17:33.48 CS.reconfigure :pressurize @14:17:33.53 CS.seek 0ml @14:17:36.54 PSV.dial PSR @14:17:37.27 delay 5 seconds @14:17:42.29 PSV.dial PTV @14:17:43.02 delay 5 seconds @14:17:48.04 PSV.dial :air @14:17:48.78 PS.seek 0ml @14:17:50.00 delay 10:00 @14:18:11.76 washCycleDA #3 of 3 @14:18:11.77 PSR.select :air @14:18:11.78 PSV.dial PSR @14:18:13.10 Pulling 0.5ml of PSR.wash @14:18:13.11 PSR.select :wash @14:18:13.23 PS.reconfigure :default @14:18:13.27 PS.seek 0.5ml @14:18:19.16 delay 5 seconds @14:18:24.18 PSR.select :air @14:18:24.30 PS.seek 2ml @14:18:40.28 PTV.dial :puck @14:18:40.30 PSV.dial PTV @14:18:41.13 PBV.dial :waste1 @14:18:41.56 PS.seek 1.5ml @14:18:47.44 delay 3 seconds @14:18:50.46 PBV.dial between PSV and waste1 @14:18:50.84 PS.seek 0ml @14:19:06.97 delay 2:00 @14:21:07.00 recoverPSRreagent08 @14:21:07.01 PTV.dial :puck @14:21:07.02 PBV.dial :waste1 @14:21:07.39 delay 30 seconds @14:21:37.42 PBV.dial between PSV and waste1 @14:21:37.78 PSR.select :air @14:21:37.80 PSV.dial PSR @14:21:38.52 PS.reconfigure :fast @14:21:38.56 PS.seek 2.5ml @14:22:01.46 PSV.dial PTV @14:22:02.20 delay 5 seconds @14:22:07.22 PSV.dial PSR @14:22:07.95 delay 5 seconds @14:22:12.97 PSV.dial PTV @14:22:13.69 delay 5 seconds @14:22:18.70 PSV.dial :air @14:22:19.44 PS.seek 0ml @14:22:42.15 Gate.power :camera,:ON @14:22:42.17 Mixing DA Substrates PSR.select :air @14:22:42.19 PSV.dial PSR @14:22:43.77 Pulling 0.25ml of PSR.sub1 @14:22:43.80 PSR.select :sub1 @14:22:44.06 PS.reconfigure :default @14:22:44.21 PS.seek 0.25ml @14:22:47.78 delay 5 seconds @14:22:52.85 Pulling 0.25ml of PSR.sub2 @14:22:52.86 PSR.select :sub2 @14:22:52.98 PS.seek 0.5ml @14:22:56.40 delay 5 seconds @14:23:01.42 PSR.select :air @14:23:01.53 PS.seek 2ml @14:23:17.62 PTV.dial :puck @14:23:17.63 PSV.dial PTV @14:23:18.35 PBV.dial :waste1 @14:23:18.77 PS.seek 1.5ml @14:23:24.74 delay 3 seconds @14:23:27.76 PBV.dial between PSV and waste1 @14:23:28.15 PS.seek 0ml @14:23:44.24 delay 10 seconds @14:23:54.27 recoverPSRreagent08 @14:23:54.28 PTV.dial :puck @14:23:54.29 PBV.dial :waste1 @14:23:54.65 delay 30 seconds @14:24:24.68 PBV.dial between PSV and waste1 @14:24:25.04 PSR.select :air @14:24:25.06 PSV.dial PSR @14:24:25.81 PS.reconfigure :fast @14:24:25.86 PS.seek 2.5ml @14:24:49.15 PSV.dial PTV @14:24:50.01 delay 5 seconds @14:24:55.03 PSV.dial PSR @14:24:55.87 delay 5 seconds @14:25:00.89 PSV.dial PTV @14:25:01.60 delay 5 seconds @14:25:06.62 PSV.dial :air @14:25:07.36 PS.seek 0ml @14:25:30.37 Additional air clear @14:25:30.38 PSR.select :air @14:25:30.40 PSV.dial PSR @14:25:31.73 Pulling 2ml of PSR.air @14:25:31.74 PSR.select :air @14:25:31.77 PS.reconfigure :default @14:25:31.81 PS.seek 2ml @14:25:52.90 delay 5 seconds @14:25:57.92 PTV.dial :puck @14:25:57.94 PSV.dial PTV @14:25:58.66 PBV.dial :waste1 @14:25:59.08 PS.seek 1.5ml @14:26:04.96 delay 3 seconds @14:26:07.98 PBV.dial between PSV and waste1 @14:26:08.35 delay 1 second @14:26:09.37 PSV.dial :waste1 @14:26:10.40 PS.seek 0ml @14:26:26.45 Processing Puck Heater at 28.1C before imaging @14:26:26.46 PH.coast @14:26:26.58 Imaging da puck in Processing Clamp while storing it to tube 2 @14:26:26.74 SC.reconfigure :tight @14:26:26.78 SC.seek 2 @14:26:30.39 SC.configure :limp @14:26:30.59 Elbow.reconfigure :tight @14:26:30.64 Elbow.seek 2 @14:26:32.94 Forearm.reconfigure :tight @14:26:32.99 Forearm.seek 2 + 700 counts @14:26:33.97 SE.reconfigure :pick @14:26:34.02 SE.seek :beamBreak @14:26:35.38 SE.configure :default @14:26:35.54 Elbow.reconfigure :limp @14:26:35.58 Forearm.reconfigure :loose @14:26:35.63 Forearm.seek 2 @14:26:36.01 Elbow.hold @14:26:36.08 Elbow.reconfigure :tight @14:26:36.17 SE.seek 28.36 Pucks below Top Plate @14:26:36.66 Forearm.force -20 @14:26:36.69 Forearm.seek 2 + 316 counts @14:26:37.01 Forearm.reconfigure :tight @14:26:37.05 Forearm.seek 2 + 700 counts @14:26:37.38 SE.seek 29.36 Pucks below Top Plate @14:26:37.97 Forearm.reconfigure :loose @14:26:38.02 Forearm.seek :retract @14:26:38.91 Elbow.seek PC @14:26:41.51 Elbow.reconfigure :loose @14:26:41.60 Forearm.seek PC @14:26:42.70 Hand.close @14:26:43.42 PC.seek :open @14:26:47.47 Forearm.seek :retract @14:26:48.51 Elbow.reconfigure :tight @14:26:48.55 Elbow.seek 2 @14:26:51.24 Forearm.reconfigure :tight @14:26:51.28 Forearm.seek 2 + 700 counts @14:26:52.33 Hand.open @14:26:53.11 SE.seek 29.01 Pucks below Top Plate @14:26:53.38 SE.reconfigure :seal @14:26:53.43 SE.seek 27.9 Pucks below Top Plate @14:26:54.03 Lights, camera, ACTION! @14:26:54.06 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23mar2314h550ml.tif @14:27:13.48 Waiting >20 seconds for da to relinquish FlushPuck @14:27:40.91 SX MX5/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 43.105 seconds /var/log/esp/da23mar2314h550ml.tif: TIFF Upload Complete @14:27:40.93 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23mar2314h550ml15s.tif @14:27:50.03 CBV.dial :waste2b @14:27:50.38 CS.reconfigure :default @14:27:50.43 CS.seek 0ml @14:27:53.15 delay 1:00 @14:27:56.67 SX MX5/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23mar2314h550ml15s.tif: TIFF Upload Complete @14:27:56.69 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da23mar2314h550ml25s.tif @14:28:22.43 SX MX5/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da23mar2314h550ml25s.tif: TIFF Upload Complete @14:28:22.44 Gate.power :camera,:OFF @14:28:22.46 DAfans.email "43.105 sec auto-exposure",Subject: "da23mar2314h550ml images!" @14:28:22.75 Elbow.seek 2 @14:28:22.94 Forearm.seek 2 + 700 counts @14:28:23.10 SE.reconfigure :gently @14:28:23.16 SE.seek 28.62 Pucks below Top Plate @14:28:23.85 Elbow.reconfigure :limp @14:28:23.96 Forearm.reconfigure :loose @14:28:24.04 Forearm.seek 2 @14:28:24.50 Elbow.hold @14:28:24.57 Elbow.reconfigure :tight @14:28:24.62 SE.reconfigure :default @14:28:24.66 SE.seek 28.8 Pucks below Top Plate @14:28:25.10 Forearm.force -20 @14:28:25.13 Forearm.seek 2 + 316 counts @14:28:25.57 Forearm.reconfigure :tight @14:28:25.66 Forearm.seek 2 + 700 counts @14:28:26.07 SE.seek :down @14:28:26.86 SE.stop @14:28:28.35 Forearm.reconfigure :loose @14:28:28.44 Forearm.seek :retract @14:28:29.55 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:28:29.91 Elbow.seek FlushPuck::Garage @14:28:31.86 Elbow.reconfigure :loose @14:28:32.10 Forearm.seek :garage @14:28:33.29 Hand.close @14:28:34.29 Forearm.seek :retract @14:28:35.49 Elbow.reconfigure :tight @14:28:35.57 Elbow.seek PC @14:28:36.99 Elbow.reconfigure :loose @14:28:37.18 Forearm.reconfigure :tight @14:28:37.27 Forearm.seek PC @14:28:38.96 PC.seek :closed @14:28:42.99 Processing Clamp onSpring/offSpring for 9/24 samples, on/off torque 1.33 @14:28:43.16 Hand.open @14:28:44.06 Forearm.reconfigure :loose @14:28:44.14 Forearm.seek :retract @14:28:45.22 PSR.select :air @14:28:45.26 PSV.dial PSR @14:28:45.86 Pulling 0.5ml of PSR.flush @14:28:45.88 PSR.select :flush @14:28:46.05 PS.seek 0.5ml @14:28:51.95 delay 5 seconds @14:28:53.18 CTV.dial :air,avoiding: :intake @14:28:54.66 Storing wcr puck in Collection Clamp to tube 2 @14:28:55.41 Elbow.reconfigure :tight @14:28:55.50 Elbow.seek 2 @14:28:57.00 PSR.select :air @14:28:57.12 PS.seek 2.5ml @14:28:57.45 upsync @14:28:58.23 Forearm.reconfigure :tight @14:28:58.28 Forearm.seek 2 + 700 counts @14:28:59.35 SE.reconfigure :pick @14:28:59.41 SE.seek :beamBreak @14:29:00.58 SE.configure :default @14:29:00.73 Elbow.reconfigure :limp @14:29:00.77 Forearm.reconfigure :loose @14:29:00.82 Forearm.seek 2 @14:29:01.21 Elbow.hold @14:29:01.28 Elbow.reconfigure :tight @14:29:01.37 SE.seek 29.36 Pucks below Top Plate @14:29:01.88 Forearm.force -20 @14:29:01.91 Forearm.seek 2 + 312 counts @14:29:02.22 Forearm.reconfigure :tight @14:29:02.27 Forearm.seek 2 + 700 counts @14:29:02.56 SE.seek 30.36 Pucks below Top Plate @14:29:03.16 Forearm.reconfigure :loose @14:29:03.20 Forearm.seek :retract @14:29:04.12 Elbow.seek CC @14:29:05.71 Elbow.reconfigure :loose @14:29:05.80 Forearm.seek CC @14:29:06.76 Hand.close @14:29:07.55 CC.seek :open @14:29:12.00 Forearm.seek :retract @14:29:13.00 Elbow.reconfigure :tight @14:29:13.55 Elbow.seek 2 @14:29:15.36 Elbow.reconfigure :limp @14:29:15.41 Forearm.seek 2 @14:29:16.54 Elbow.hold @14:29:16.61 Elbow.reconfigure :tight @14:29:16.66 Hand.open @14:29:17.54 Forearm.force -20 @14:29:17.58 Forearm.seek 2 + 312 counts @14:29:17.93 Forearm.reconfigure :tight @14:29:17.98 Forearm.seek 2 + 700 counts @14:29:18.29 delay 1 second @14:29:18.33 SE.seek :down @14:29:19.00 SE.stop @14:29:19.41 PRV.dial :waste2,avoiding: :ab2 @14:29:19.49 Forearm.reconfigure :loose @14:29:19.53 Forearm.seek :retract @14:29:20.44 Starting finalPurge @14:29:21.22 PTV.dial :PRVmixing @14:29:22.48 PSV.dial :mixloop @14:29:23.47 PS.seek :empty @14:29:39.59
Waiting >20 seconds for psFlush to relinquish FlushPuck @14:29:49.70 PSR.select :air @14:29:49.72 PSV.dial PSR @14:29:50.90 Pulling 0.5ml of PSR.flush @14:29:50.91 PSR.select :flush @14:29:51.03 PS.seek 0.5ml @14:29:56.89 delay 5 seconds @14:30:01.91 PSR.select :air @14:30:02.03 PS.seek 2.5ml @14:30:23.08 delay 1 second @14:30:24.10 PBV.dial :waste1 @14:30:24.47 PTV.dial :mixing @14:30:25.19 PSV.dial :mixloop @14:30:26.18 PS.seek :empty @14:30:52.39 PSV.dial PSR @14:30:53.39 PSR.select :flush @14:30:53.51 PS.seek 1ml @14:31:04.51 PSR.select :air @14:31:04.63 PS.seek 2.5ml @14:31:20.79 PSV.dial PTV @14:31:21.64 PTV.dial :puck @14:31:22.34 PBV.dial :waste1 @14:31:22.36 PS.seek 0ml @14:31:48.61 PSV.dial PSR @14:31:49.33 PSR.select :flush @14:31:49.44 PS.seek 0.5ml @14:31:55.29 PSR.select :air @14:31:55.41 PS.seek 2.5ml @14:32:16.54 PSV.dial PTV @14:32:17.28 PBV.dial PSV @14:32:17.83 PS.seek 0ml @14:32:43.95 PSV.dial PBV @14:32:44.41 PS.seek 2.5ml @14:33:10.57 PSV.dial :waste1 @14:33:11.30 PS.seek 0ml @14:33:37.51 PSV.dial PSR @14:33:37.96 PSR.select :air @14:33:37.99 PS.seek 2.5ml @14:34:04.13 PSV.dial PTV @14:34:04.85 PBV.dial PSV @14:34:04.87 PS.seek 0ml @14:34:31.13 PSV.dial PBV @14:34:31.59 PS.seek 2.5ml @14:34:57.95 PSV.dial :waste1 @14:34:58.67 PS.seek 0ml @14:35:24.79 PSV.dial PSR @14:35:25.35 PSR.select :flush @14:35:25.47 PS.seek 1ml @14:35:36.47 PSR.select :air @14:35:36.59 PS.seek 2.5ml @14:35:52.57 PSV.dial PTV @14:35:53.30 PTV.dial :puck @14:35:53.32 PBV.dial :waste1 @14:35:53.86 PS.seek 0ml @14:36:20.04 PSV.dial PSR @14:36:20.77 PSR.select :flush @14:36:20.89 PS.seek 0.5ml @14:36:26.79 PSR.select :air @14:36:26.90 PS.seek 2.5ml @14:36:48.00 PSV.dial PTV @14:36:49.19 PBV.dial PSV @14:36:49.75 PS.seek 0ml @14:37:15.97 PSV.dial PBV @14:37:16.51 PS.seek 2.5ml @14:37:42.63 PSV.dial :waste1 @14:37:43.36 PS.seek 0ml @14:38:09.60 PSV.dial PSR @14:38:10.05 PSR.select :air @14:38:10.08 PS.seek 2.5ml @14:38:36.19 PSV.dial PTV @14:38:36.91 PBV.dial PSV @14:38:36.93 PS.seek 0ml @14:39:03.06 PSV.dial PBV @14:39:03.52 PS.seek 2.5ml @14:39:29.66 PSV.dial :waste1 @14:39:30.77 PS.seek 0ml @14:39:56.94
Loading FlushPuck into Collection Clamp from Processing Clamp @14:39:57.12 Elbow.seek PC @14:39:59.86 Elbow.reconfigure :loose @14:39:59.95 Forearm.seek PC @14:40:01.02 Hand.close @14:40:01.65 PC.seek :open @14:40:05.61 Forearm.seek :retract @14:40:06.65 Elbow.reconfigure :tight @14:40:06.69 Elbow.seek CC @14:40:10.28 Elbow.reconfigure :loose @14:40:10.36 Forearm.reconfigure :tight @14:40:10.41 Forearm.seek CC @14:40:11.61 CC.seek :closed @14:40:15.84 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.74 @14:40:15.91 Hand.open @14:40:16.71 Forearm.reconfigure :loose @14:40:16.75 Forearm.seek :retract @14:40:17.74 Flushing the Collection Valve::Manifold @14:40:17.86 CSV.dial CSR @14:40:18.35 Pulling 5ml of CSR.flush CSR.select :flush @14:40:18.46 CS.seek 5ml @14:40:31.92 delay 5 seconds @14:40:36.93 CSR.select :air @14:40:37.08 CS.reconfigure :fast @14:40:37.13 CS.seek 7.5ml @14:40:43.54 CSV.dial :waste @14:40:44.81 CS.reconfigure :default @14:40:44.85 CS.seek 0ml @14:41:04.57 Flushing lower manifold's waste1 port @14:41:04.69 CSV.dial :flush @14:41:05.12 CS.reconfigure :fast @14:41:05.16 CS.seek 5ml @14:41:17.01 delay 5 seconds @14:41:22.03 CSV.dial :air @14:41:22.52 CS.seek 7ml @14:41:27.80 CSV.dial CTV @14:41:27.82
CTV.dial CSV @14:41:29.16 CBV.dial :waste1 @14:41:29.90 CS.seek 0ml @14:41:45.98 Flushing lower manifold's waste2 port @14:41:46.09 CSV.dial :flush @14:41:46.81 CS.seek 5ml @14:41:58.60 delay 5 seconds @14:42:03.62 CSV.dial :air @14:42:04.13 CS.seek 6.999ml @14:42:09.41 CSV.dial CTV @14:42:09.46
CTV.dial CSV @14:42:09.87 CBV.dial :waste2 @14:42:10.89 CS.seek 0ml @14:42:27.04 Flushing lower manifold's waste2b port @14:42:27.15 CSV.dial :flush @14:42:27.89 CS.seek 5ml @14:42:39.75 delay 5 seconds @14:42:44.77 CSV.dial :air @14:42:45.28 CS.seek 6.999ml @14:42:50.57 CSV.dial CTV @14:42:50.59
CTV.dial CSV @14:42:51.04 CBV.dial :waste2b @14:42:52.51 CS.seek 0ml @14:43:08.63 Disinfecting external Intake with kill @14:43:08.75 CSV.dial CSR @14:43:09.23 Pulling 8ml of CSR.kill @14:43:09.24 CSR.select :kill @14:43:09.35 CS.seek 8ml @14:43:27.79 delay 5 seconds @14:43:32.80 CSR.select :air @14:43:32.92 CS.seek 10ml @14:43:38.19 CSV.dial CBV @14:43:38.22
CBV.dial CSV @14:43:39.38 CTV.dial :intake @14:43:40.10 Intake.select :open @14:43:40.22 CS.seek 2ml @14:43:58.38 Intake.select :close @14:43:58.48 CTV.dial :air @14:43:59.23 CS.reconfigure :default @14:43:59.27 CS.seek 0ml @14:44:05.22 CSV.dial CSR @14:44:06.28 Pulling 5ml of CSR.flush CSR.select :flush @14:44:06.40 CS.seek 5ml @14:44:19.78 delay 5 seconds @14:44:24.80 CSR.select :air @14:44:24.92 CS.seek 10ml @14:44:38.37 CSV.dial CBV @14:44:39.49 CS.seek 0ml @14:45:05.56 Garaging FlushPuck in Collection Clamp @14:45:05.63 Elbow.reconfigure :tight @14:45:05.68 Elbow.seek CC @14:45:05.78 Elbow.reconfigure :loose @14:45:05.87 Forearm.seek CC @14:45:06.87 Hand.close @14:45:07.64 CC.seek :open @14:45:11.95 Forearm.seek :retract @14:45:12.95 Elbow.reconfigure :tight @14:45:12.99 Elbow.seek :garage @14:45:15.91 Elbow.reconfigure :loose @14:45:16.00 Forearm.reconfigure :tight @14:45:16.04 Forearm.seek :garage @14:45:17.15 Hand.open @14:45:17.95 Forearm.reconfigure :loose @14:45:17.99 Forearm.seek :retract @14:45:19.04 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 12:24:36 @14:45:19.09 Asleep after completing DA_WCR_032223 assays @14:45:19.11 GoodNews.email "{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@14:43:40, 17.6C, 53% humidity, 15.5psia, 13.933V, 0.349A, 0.349A avg, 4.86W\nSampled 250.0ml",Subject: "Asleep after completing DA_WCR_032223 assays" @14:45:23.04 upsync @12:45:39.82UTC30-Mar-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @12:45:42.39 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @12:45:46.31 Polling Can every 10:00 @12:45:46.45 Cleanly exited at 12:43:25UTC30-Mar-23 @12:45:46.48 Loading /home/esp/esp2/mission/DA_WCR_033023.rb mission @12:45:46.53 GoodNews.email "Starting DA_WCR_033023 w/pucks in tube 3\n{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:45:46, 17.3C, 54% humidity, 15.5psia, 14.002V, 0.220A, 0.220A avg, 3.09W",Subject: "DA_WCR_033023 begun" @12:45:46.57
Preparing to sleep until + 00:00:05 @12:45:46.60 Asleep til + 00:00:05 @12:45:46.62 Continuing at 12:45 @12:45:46.90 Gate.power :core,:OFF @12:45:46.91 Gate.power :raw,:OFF @12:45:46.92 Shutdown @12:45:49.96 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_033023.rb:14\n{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:45:46, 17.4C, 54% humidity, 15.5psia, 14.002V, 0.220A, 0.220A avg, 3.09W",Subject: "Asleep until 12:45" @12:45:51.36 upsync @12:45:51.51
Powering core bus @12:45:53.91 upsync @12:45:56.91
Gate.power :raw,:ON @12:45:57.55 CS.configure :default @12:45:57.63 PS.configure :default @12:45:57.72 SS.configure :default @12:45:57.81 CC.configure :default @12:45:57.90 PC.configure :default @12:45:58.00 Forearm.configure :loose @12:45:58.09 Elbow.configure :loose @12:45:58.19 SC.configure :loose @12:45:58.28 SE.configure :default @12:45:58.32 CH.coast @12:45:58.37 CH.configure :default @12:45:58.40 PH.coast @12:45:58.46 PH.configure :default @12:45:58.49 All dwarf controllers running firmware version 3.85 @12:45:58.60 Valve.reset @12:45:58.82 Intake.select :close @12:45:58.85 Exhaust.select :close @12:45:58.88 CTV.dial :air @12:45:58.97 PSV.dial :air @12:45:59.06 CSV.dial :waste1 @12:45:59.09
Forearm.reconfigure :loose @12:45:59.16 Forearm.home.seek :retract @12:45:59.55 SSV.dial :spit,avoiding: :exhaust @12:45:59.91 Hand.close @12:45:59.94
Elbow.jog 1500 @12:46:00.04 PS.reconfigure :default @12:46:00.09 PS.home.seek 0ml @12:46:00.36 CS.reconfigure :fast @12:46:00.39 SS.reconfigure :default @12:46:00.47 CS.home.seek 0ml @12:46:00.51 SS.home.seek 0ml @12:46:00.62
Elbow.reconfigure :loose @12:46:00.66 Elbow.home.seek :clear @12:46:03.87 SE.reconfigure :default @12:46:03.91 SE.home.seek :down @12:46:04.56 SC.reconfigure :tight @12:46:04.60 SC.home.seek :initial @12:46:08.68 PS.stop @12:46:34.65 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @12:46:34.66
Retry #1 of 3 @12:46:34.72 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @12:46:39.10 SC.configure :limp @12:46:39.15 Forearm.seek :retract @12:46:39.21 Elbow.seek :hammer @12:46:40.33 Forearm.reconfigure :tight @12:46:40.37 Forearm.seek retracted @12:46:40.59 PC.reconfigure :default @12:46:40.61
CC.reconfigure :default @12:46:40.68 PC.home.seek :open @12:46:40.71
CC.home.seek :open @12:46:43.95 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @12:46:43.97 Notice.email "At 12:46:43 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_033023.rb:14\n{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:46:43, 17.4C, 54% humidity, 15.5psia, 13.850V, 0.484A, 0.484A avg, 6.70W",Subject: "Started DA_WCR_033023 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @12:46:43.99
Starting initialPurge @12:46:44.01 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:46:44.08 Hand.open @12:46:44.98 Elbow.reconfigure :tight @12:46:45.02 Elbow.seek FlushPuck::Garage @12:46:47.01 Elbow.reconfigure :loose @12:46:47.21 Forearm.reconfigure :loose @12:46:47.26 Forearm.seek :garage @12:46:48.27 upsync @12:46:48.39
Hand.close @12:46:49.21 Forearm.seek :retract @12:46:50.29 Elbow.reconfigure :tight @12:46:50.33 Elbow.seek CC @12:46:53.24 Elbow.reconfigure :loose @12:46:53.33 Forearm.reconfigure :tight @12:46:53.38 Forearm.seek CC @12:46:54.66 CC.seek :closed @12:46:58.90 Collection Clamp onSpring/offSpring for 8/25 samples, on/off torque 1.84 @12:46:58.97 Hand.open @12:46:59.78 Forearm.reconfigure :loose @12:46:59.82 Forearm.seek :retract @12:47:00.81 Flushing the Collection Valve::Manifold @12:47:00.93 CSV.dial CSR @12:47:02.25 Pulling 5ml of CSR.flush CSR.select :flush @12:47:02.36 CS.reconfigure :default @12:47:02.40 CS.seek 5ml @12:47:15.88 delay 5 seconds @12:47:20.90 CSR.select :air @12:47:21.05 CS.reconfigure :fast @12:47:21.10 CS.seek 7.5ml @12:47:27.50 CSV.dial :waste @12:47:28.77 CS.reconfigure :default @12:47:28.82 CS.seek 0ml @12:47:48.50 Purging external Intake @12:47:48.62 CSV.dial :flush @12:47:49.06 CS.reconfigure :fast @12:47:49.10 CS.seek 10ml @12:48:12.10 delay 5 seconds @12:48:17.12 CSV.dial CBV @12:48:17.18
CBV.dial CSV @12:48:17.86 CTV.dial :intake @12:48:18.60 Intake.select :open @12:48:18.70 CS.seek 0ml @12:48:41.56 Intake.select :close @12:48:41.66 CTV.dial :air @12:48:42.42 Flushing upper manifold @12:48:42.53 CSV.dial :flush @12:48:43.26 CS.seek 5ml @12:48:55.25 delay 5 seconds @12:49:00.27 CSV.dial :air @12:49:00.76 CS.seek 7ml @12:49:06.01 CSV.dial CBV @12:49:06.03
CBV.dial CSV @12:49:07.05 CTV.dial :air @12:49:07.07 CS.seek 0ml @12:49:23.25 delay 7 seconds @12:49:30.28 CS.seek 3ml @12:49:37.75 CSV.dial :waste @12:49:38.19 CS.seek 0ml @12:49:45.79 CSV.dial :flush @12:49:46.23 CS.seek 3ml @12:49:53.69 delay 5 seconds @12:49:58.70 CSV.dial :air @12:49:59.20 CS.seek 5.001ml @12:50:04.50 CSV.dial :air @12:50:04.52 CS.seek 0ml @12:50:16.34 delay 7 seconds @12:50:23.37 CS.seek 3ml @12:50:30.87 CSV.dial :waste @12:50:31.60 CS.seek 0ml @12:50:39.09 Garaging FlushPuck in Collection Clamp @12:50:39.16 Elbow.reconfigure :tight @12:50:39.20 Elbow.seek CC @12:50:39.31 Elbow.reconfigure :loose @12:50:39.40 Forearm.seek CC @12:50:40.35 Hand.close @12:50:41.09 CC.seek :open @12:50:45.42 Forearm.seek :retract @12:50:46.42 Elbow.reconfigure :tight @12:50:46.46 Elbow.seek :garage @12:50:49.41 Elbow.reconfigure :loose @12:50:49.50 Forearm.reconfigure :tight @12:50:49.54 Forearm.seek :garage @12:50:50.66 Hand.open @12:50:51.83 Forearm.reconfigure :loose @12:50:51.87 Forearm.seek :retract @12:50:52.92 da sampling at most 1000ml, wcr at most 1000ml @12:50:52.95 Loading sh1 puck from tube 3 into Collection Clamp @12:50:52.96 DAfans.email "Started da in tube 3 at 12:50:52UTC30-Mar-23\n{1 => 33,\n 2 => 31,\n 3 => 2,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@12:46:43, 17.4C, 54% humidity, 15.5psia, 13.850V, 0.484A, 0.484A avg, 6.70W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @12:50:53.21
SC.reconfigure :tight @12:50:53.26 SC.seek 3 @12:50:56.88 upsync @12:51:03.10
SC.configure :limp @12:51:03.31 Elbow.reconfigure :tight @12:51:03.35 Elbow.seek 3 @12:51:05.33 Forearm.reconfigure :tight @12:51:05.37 Forearm.seek 3 + 700 counts @12:51:06.37 SE.reconfigure :pick @12:51:06.42 SE.seek :beamBreak @12:51:09.49 SE.configure :default @12:51:09.65 Elbow.seek 3 @12:51:09.82 Forearm.seek 3 + 300 counts @12:51:10.04 Forearm.reconfigure :loose @12:51:10.08 Hand.close @12:51:10.73 SE.seek :down @12:51:10.91
Forearm.seek :retract @12:51:11.92 Elbow.seek CC @12:51:13.45 SE.stop @12:51:13.55
Elbow.reconfigure :loose @12:51:13.64 Forearm.reconfigure :tight @12:51:13.69 Forearm.seek CC @12:51:14.98 CC.seek :closed @12:51:19.22 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.65 @12:51:19.23 Hand.open @12:51:20.03 Forearm.reconfigure :loose @12:51:20.07 Forearm.seek :retract @12:51:21.09 collectSample 1000ml w/bubble point of 24psi @12:51:21.10 Priming 7ml @12:51:21.11 Intake.select :close @12:51:21.12 Exhaust.select :close @12:51:21.13 SS.hold @12:51:21.32 Calibrating pressure and temperature sensors @12:51:21.35 CTV.dial :air @12:51:21.36 SSV.dial :spit,avoiding: :exhaust @12:51:21.38 SS.reconfigure :fast @12:51:21.42 SS.seek 5ml @12:51:24.32 SSV.dial CBV @12:51:24.36
CBV.dial SSV @12:51:25.65 delay 3.8 seconds @12:51:29.57 SSV.dial :spit @12:51:30.86 SS.seek 0ml @12:51:33.56 SSV.dial CBV @12:51:34.84 CTV.dial :intake @12:51:35.57 SSV.dial CBV @12:51:35.59
CBV.dial SSV @12:51:35.60 Allowing 40s for 7.0ml of remaining 7.0ml @12:51:35.61 Intake.select :open @12:51:37.76 Intake @15.2psia, vacuum from 9.7 to 12.7psi @12:51:37.78 SS.reconfigure :inhaling 2.14ml/s @12:51:37.82 SS.seek 7ml @12:51:44.46 Stroke completed -- waiting for pressure to equalize ... @12:51:44.47 SS.reconfigure :exhale @12:51:44.51 SS.hold @12:51:47.43 Intake.select :close @12:51:47.57 SSV.dial :exhaust @12:51:48.31 Exhaust.select :open @12:51:48.41 SS.seek 0ml @12:51:52.24 Exhaust.select :close @12:51:52.35 Sampling 1000.0ml Intake.select :close @12:51:52.36 Exhaust.select :close @12:51:52.37 SS.reconfigure :default @12:51:52.41 SS.hold @12:51:52.59 CTV.dial :intake @12:51:52.61 SSV.dial CBV @12:51:52.63
CBV.dial SSV @12:51:53.38 Allowing 130s for 25.0ml of remaining 1000.0ml @12:51:53.39 Intake.select :open @12:51:55.54 Intake @15.2psia, vacuum from 9.7 to 12.7psi @12:51:55.56 SS.reconfigure :inhaling 2.14ml/s @12:51:55.61 SS.seek 25ml @12:52:01.48 Vacuum too high -- waiting up to 40 seconds @12:52:01.49 SS.reconfigure :exhale @12:52:01.54 SS.hold @12:52:02.22 Intake @10.6psia, vacuum from 5.1 to 8.1psi @12:52:02.23 SS.reconfigure :inhaling 1.93ml/s @12:52:02.28 SS.seek 25ml @12:52:05.46 Vacuum too high -- waiting up to 40 seconds @12:52:05.47 SS.reconfigure :exhale @12:52:05.51 SS.hold @12:52:06.72 Intake @14.5psia, vacuum from 9.0 to 12.0psi @12:52:06.73 SS.reconfigure :inhaling 1.74ml/s @12:52:06.78 SS.seek 25ml @12:52:09.89 Vacuum too high -- waiting up to 40 seconds @12:52:09.90 SS.reconfigure :exhale @12:52:09.94 SS.hold @12:52:10.55 Intake @11.6psia, vacuum from 6.1 to 9.1psi @12:52:10.56 SS.reconfigure :inhaling 1.57ml/s @12:52:10.61 SS.seek 25ml @12:52:13.27 Vacuum too high -- waiting up to 40 seconds @12:52:13.28 SS.reconfigure :exhale @12:52:13.32 SS.hold @12:52:14.24 Intake @13.5psia, vacuum from 8.0 to 11.0psi @12:52:14.26 SS.reconfigure :inhaling 1.41ml/s @12:52:14.30 SS.seek 25ml @12:52:17.67 Vacuum too high -- waiting up to 40 seconds @12:52:17.68 SS.reconfigure :exhale @12:52:17.72 SS.hold @12:52:18.22 Intake @11.7psia, vacuum from 6.2 to 9.2psi @12:52:18.23 SS.reconfigure :inhaling 1.27ml/s @12:52:18.28 SS.seek 25ml @12:52:21.23 Vacuum too high -- waiting up to 40 seconds @12:52:21.24 SS.reconfigure :exhale @12:52:21.28 SS.hold @12:52:22.03 Intake @13.4psia, vacuum from 7.9 to 10.9psi @12:52:22.05 SS.reconfigure :inhaling 1.14ml/s @12:52:22.09 SS.seek 25ml @12:52:25.02 Stroke completed -- waiting for pressure to equalize ... @12:52:25.03 SS.reconfigure :exhale @12:52:25.07 SS.hold @12:52:25.64 Intake.select :close @12:52:25.78 SSV.dial :exhaust @12:52:26.52 Exhaust.select :open @12:52:26.63 SS.seek 0ml @12:52:35.52 Exhaust.select :close @12:52:35.63 SSV.dial CBV @12:52:35.65
CBV.dial SSV @12:52:36.40 Allowing 130s for 25.0ml of remaining 975.0ml @12:52:36.41 Intake.select :open @12:52:38.56 Intake @14.9psia, vacuum from 9.4 to 12.4psi @12:52:38.58 SS.reconfigure :inhaling 1.14ml/s @12:52:38.62 SS.seek 25ml @12:53:03.29 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @12:53:03.33 SS.hold @12:53:04.20 Intake.select :close @12:53:04.34 SSV.dial :exhaust @12:53:05.07 Exhaust.select :open @12:53:05.18 SS.seek 0ml @12:53:14.33 Exhaust.select :close @12:53:14.44 SSV.dial CBV @12:53:14.46
CBV.dial SSV @12:53:15.21 Allowing 130s for 25.0ml of remaining 950.0ml @12:53:15.22 Intake.select :open @12:53:17.37 Intake @14.6psia, vacuum from 9.1 to 12.1psi @12:53:17.39 SS.reconfigure :inhaling 1.14ml/s @12:53:17.43 SS.seek 25ml @12:53:38.32 Vacuum too high -- waiting up to 40 seconds @12:53:38.33 SS.reconfigure :exhale @12:53:38.38 SS.hold @12:53:38.80 Intake @11.0psia, vacuum from 5.5 to 8.5psi @12:53:38.81 SS.reconfigure :inhaling 1.03ml/s @12:53:38.85 SS.seek 25ml @12:53:42.13 Stroke completed -- waiting for pressure to equalize ... @12:53:42.14 SS.reconfigure :exhale @12:53:42.18 SS.hold @12:53:43.03 Intake.select :close @12:53:43.17 SSV.dial :exhaust @12:53:43.91 Exhaust.select :open @12:53:44.02 SS.seek 0ml @12:53:53.22 Exhaust.select :close @12:53:53.33 SSV.dial CBV @12:53:53.35
CBV.dial SSV @12:53:54.10 Allowing 130s for 25.0ml of remaining 925.0ml @12:53:54.11 Intake.select :open @12:53:56.26 Intake @14.6psia, vacuum from 9.1 to 12.1psi @12:53:56.28 SS.reconfigure :inhaling 1.03ml/s @12:53:56.32 SS.seek 25ml @12:54:22.17 Stroke completed -- waiting for pressure to equalize ... @12:54:22.18 SS.reconfigure :exhale @12:54:22.22 SS.hold @12:54:23.09 Intake.select :close @12:54:23.23 SSV.dial :exhaust @12:54:23.96 Exhaust.select :open @12:54:24.06 SS.seek 0ml @12:54:33.31 Exhaust.select :close @12:54:33.42 SSV.dial CBV @12:54:33.44
CBV.dial SSV @12:54:34.18 Allowing 130s for 25.0ml of remaining 900.0ml @12:54:34.19 Intake.select :open @12:54:36.34 Intake @14.5psia, vacuum from 9.0 to 12.0psi @12:54:36.35 SS.reconfigure :inhaling 1.03ml/s @12:54:36.39 SS.seek 25ml @12:55:02.21 Stroke completed -- waiting for pressure to equalize ... @12:55:02.22 SS.reconfigure :exhale @12:55:02.26 SS.hold @12:55:03.21 Intake.select :close @12:55:03.35 SSV.dial :exhaust @12:55:04.10 Exhaust.select :open @12:55:04.20 SS.seek 0ml @12:55:13.13 Exhaust.select :close @12:55:13.23 SSV.dial CBV @12:55:13.25
CBV.dial SSV @12:55:13.99 Allowing 130s for 25.0ml of remaining 875.0ml @12:55:14.00 Intake.select :open @12:55:16.15 Intake @14.5psia, vacuum from 9.0 to 12.0psi @12:55:16.16 SS.reconfigure :inhaling 1.03ml/s @12:55:16.21 SS.seek 25ml @12:55:42.91 Stroke completed -- waiting for pressure to equalize ... @12:55:42.92 SS.reconfigure :exhale @12:55:42.97 SS.hold @12:55:44.14 Intake.select :close @12:55:44.28 SSV.dial :exhaust @12:55:45.02 Exhaust.select :open @12:55:45.12 SS.seek 0ml @12:55:53.95 Exhaust.select :close @12:55:54.05 SSV.dial CBV @12:55:54.07
CBV.dial SSV @12:55:54.81 Allowing 130s for 25.0ml of remaining 850.0ml @12:55:54.82 Intake.select :open @12:55:56.98 Intake @14.4psia, vacuum from 8.9 to 11.9psi @12:55:56.99 SS.reconfigure :inhaling 1.03ml/s @12:55:57.03 SS.seek 25ml @12:56:23.70 Stroke completed -- waiting for pressure to equalize ... @12:56:23.71 SS.reconfigure :exhale @12:56:23.75 SS.hold @12:56:25.08 Intake.select :close @12:56:25.22 SSV.dial :exhaust @12:56:25.95 Exhaust.select :open @12:56:26.06 SS.seek 0ml @12:56:34.91 Exhaust.select :close @12:56:35.01 SSV.dial CBV @12:56:35.03
CBV.dial SSV @12:56:35.77 Allowing 130s for 25.0ml of remaining 825.0ml @12:56:35.78 Intake.select :open @12:56:37.94 Intake @14.4psia, vacuum from 8.9 to 11.9psi @12:56:37.95 SS.reconfigure :inhaling 1.03ml/s @12:56:38.00 SS.seek 25ml @12:57:04.57 Stroke completed -- waiting for pressure to equalize ... @12:57:04.58 SS.reconfigure :exhale @12:57:04.63 SS.hold @12:57:06.18 Intake.select :close @12:57:06.32 SSV.dial :exhaust @12:57:07.06 Exhaust.select :open @12:57:07.17 SS.seek 0ml @12:57:16.51 Exhaust.select :close @12:57:16.62 SSV.dial CBV @12:57:16.64
CBV.dial SSV @12:57:17.37 Allowing 130s for 25.0ml of remaining 800.0ml @12:57:17.38 Intake.select :open @12:57:19.54 Intake @14.3psia, vacuum from 8.8 to 11.8psi @12:57:19.55 SS.reconfigure :inhaling 1.03ml/s @12:57:19.59 SS.seek 25ml @12:57:45.43 Stroke completed -- waiting for pressure to equalize ... @12:57:45.45 SS.reconfigure :exhale @12:57:45.49 SS.hold @12:57:47.12 Intake.select :close @12:57:47.26 SSV.dial :exhaust @12:57:48.00 Exhaust.select :open @12:57:48.10 SS.seek 0ml @12:57:57.04 Exhaust.select :close @12:57:57.15 SSV.dial CBV @12:57:57.17
CBV.dial SSV @12:57:57.92 Allowing 130s for 25.0ml of remaining 775.0ml @12:57:57.93 Intake.select :open @12:58:00.08 Intake @14.3psia, vacuum from 8.8 to 11.8psi @12:58:00.10 SS.reconfigure :inhaling 1.03ml/s @12:58:00.14 SS.seek 25ml @12:58:22.44 Vacuum too high -- waiting up to 40 seconds @12:58:22.45 SS.reconfigure :exhale @12:58:22.49 SS.hold @12:58:22.90 Intake @11.3psia, vacuum from 5.8 to 8.8psi @12:58:22.91 SS.reconfigure :inhaling 0.93ml/s @12:58:22.96 SS.seek 25ml @12:58:28.32 Stroke completed -- waiting for pressure to equalize ... @12:58:28.33 SS.reconfigure :exhale @12:58:28.37 SS.hold @12:58:29.55 Intake.select :close @12:58:29.69 SSV.dial :exhaust @12:58:30.43 Exhaust.select :open @12:58:30.54 SS.seek 0ml @12:58:39.42 Exhaust.select :close @12:58:39.52 SSV.dial CBV @12:58:39.54
CBV.dial SSV @12:58:40.29 Allowing 130s for 25.0ml of remaining 750.0ml @12:58:40.30 Intake.select :open @12:58:42.46 Intake @14.3psia, vacuum from 8.8 to 11.8psi @12:58:42.47 SS.reconfigure :inhaling 0.93ml/s @12:58:42.51 SS.seek 25ml @12:59:11.84 Stroke completed -- waiting for pressure to equalize ... @12:59:11.85 SS.reconfigure :exhale @12:59:11.89 SS.hold @12:59:13.16 Intake.select :close @12:59:13.31 SSV.dial :exhaust @12:59:14.04 Exhaust.select :open @12:59:14.15 SS.seek 0ml @12:59:23.15 Exhaust.select :close @12:59:23.25 SSV.dial CBV @12:59:23.28
CBV.dial SSV @12:59:24.04 Allowing 130s for 25.0ml of remaining 725.0ml @12:59:24.05 Intake.select :open @12:59:26.21 Intake @14.3psia, vacuum from 8.8 to 11.8psi @12:59:26.22 SS.reconfigure :inhaling 0.93ml/s @12:59:26.27 SS.seek 25ml @12:59:55.59 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @12:59:55.63 SS.hold @12:59:57.61 Intake.select :close @12:59:57.75 SSV.dial :exhaust @12:59:58.49 Exhaust.select :open @12:59:58.60 SS.seek 0ml @13:00:07.84 Exhaust.select :close @13:00:07.94 SSV.dial CBV @13:00:07.96
CBV.dial SSV @13:00:08.71 Allowing 130s for 25.0ml of remaining 700.0ml @13:00:08.72 Intake.select :open @13:00:10.88 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:00:10.89 SS.reconfigure :inhaling 0.93ml/s @13:00:10.94 SS.seek 25ml @13:00:39.38 Stroke completed -- waiting for pressure to equalize ... @13:00:39.39 SS.reconfigure :exhale @13:00:39.43 SS.hold @13:00:41.83 Intake.select :close @13:00:41.97 SSV.dial :exhaust @13:00:42.71 Exhaust.select :open @13:00:42.82 SS.seek 0ml @13:00:51.80 Exhaust.select :close @13:00:51.91 SSV.dial CBV @13:00:51.94
CBV.dial SSV @13:00:52.65 Allowing 130s for 25.0ml of remaining 675.0ml @13:00:52.66 Intake.select :open @13:00:54.81 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:00:54.83 SS.reconfigure :inhaling 0.93ml/s @13:00:54.87 SS.seek 25ml @13:01:22.00 Vacuum too high -- waiting up to 40 seconds @13:01:22.01 SS.reconfigure :exhale @13:01:22.05 SS.hold @13:01:23.34 Intake @12.8psia, vacuum from 7.3 to 10.3psi @13:01:23.35 SS.reconfigure :inhaling 0.84ml/s @13:01:23.40 SS.seek 25ml @13:01:26.49 Stroke completed -- waiting for pressure to equalize ... @13:01:26.50 SS.reconfigure :exhale @13:01:26.54 SS.hold @13:01:27.74 Intake.select :close @13:01:27.89 SSV.dial :exhaust @13:01:28.63 Exhaust.select :open @13:01:28.74 SS.seek 0ml @13:01:37.71 Exhaust.select :close @13:01:37.81 SSV.dial CBV @13:01:37.83
CBV.dial SSV @13:01:38.57 Allowing 130s for 25.0ml of remaining 650.0ml @13:01:38.58 Intake.select :open @13:01:40.73 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:01:40.75 SS.reconfigure :inhaling 0.84ml/s @13:01:40.79 SS.seek 25ml @13:02:12.78 Stroke completed -- waiting for pressure to equalize ... @13:02:12.79 SS.reconfigure :exhale @13:02:12.84 SS.hold @13:02:15.23 Intake.select :close @13:02:15.38 SSV.dial :exhaust @13:02:16.11 Exhaust.select :open @13:02:16.22 SS.seek 0ml @13:02:25.64 Exhaust.select :close @13:02:25.75 SSV.dial CBV @13:02:25.77
CBV.dial SSV @13:02:26.51 Allowing 130s for 25.0ml of remaining 625.0ml @13:02:26.52 Intake.select :open @13:02:28.67 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:02:28.68 SS.reconfigure :inhaling 0.84ml/s @13:02:28.73 SS.seek 25ml @13:02:59.83 Stroke completed -- waiting for pressure to equalize ... @13:02:59.84 SS.reconfigure :exhale @13:02:59.88 SS.hold @13:03:02.28 Intake.select :close @13:03:02.42 SSV.dial :exhaust @13:03:03.16 Exhaust.select :open @13:03:03.27 SS.seek 0ml @13:03:12.28 Exhaust.select :close @13:03:12.39 SSV.dial CBV @13:03:12.41
CBV.dial SSV @13:03:13.14 Allowing 130s for 25.0ml of remaining 600.0ml @13:03:13.15 Intake.select :open @13:03:15.31 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:03:15.32 SS.reconfigure :inhaling 0.84ml/s @13:03:15.36 SS.seek 25ml @13:03:42.06 Vacuum too high -- waiting up to 40 seconds @13:03:42.07 SS.reconfigure :exhale @13:03:42.11 SS.hold @13:03:43.74 Intake @13.7psia, vacuum from 8.2 to 11.2psi @13:03:43.75 SS.reconfigure :inhaling 0.76ml/s @13:03:43.80 SS.seek 25ml @13:03:50.17 Stroke completed -- waiting for pressure to equalize ... @13:03:50.18 SS.reconfigure :exhale @13:03:50.22 SS.hold @13:03:52.26 Intake.select :close @13:03:52.40 SSV.dial :exhaust @13:03:53.14 Exhaust.select :open @13:03:53.25 SS.seek 0ml @13:04:02.23 Exhaust.select :close @13:04:02.34 SSV.dial CBV @13:04:02.36
CBV.dial SSV @13:04:03.11 Allowing 130s for 25.0ml of remaining 575.0ml @13:04:03.12 Intake.select :open @13:04:05.28 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:04:05.29 SS.reconfigure :inhaling 0.76ml/s @13:04:05.34 SS.seek 25ml @13:04:40.69 Stroke completed -- waiting for pressure to equalize ... @13:04:40.70 SS.reconfigure :exhale @13:04:40.74 SS.hold @13:04:43.63 Intake.select :close @13:04:43.77 SSV.dial :exhaust @13:04:44.52 Exhaust.select :open @13:04:44.62 SS.seek 0ml @13:04:53.64 Exhaust.select :close @13:04:53.74 SSV.dial CBV @13:04:53.77
CBV.dial SSV @13:04:54.52 Allowing 130s for 25.0ml of remaining 550.0ml @13:04:54.53 Intake.select :open @13:04:56.68 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:04:56.69 SS.reconfigure :inhaling 0.76ml/s @13:04:56.74 SS.seek 25ml @13:05:32.11 Stroke completed -- waiting for pressure to equalize ... @13:05:32.12 SS.reconfigure :exhale @13:05:32.16 SS.hold @13:05:35.89 Intake.select :close @13:05:36.03 SSV.dial :exhaust @13:05:36.77 Exhaust.select :open @13:05:36.87 SS.seek 0ml @13:05:45.89 Exhaust.select :close @13:05:45.99 SSV.dial CBV @13:05:46.02
CBV.dial SSV @13:05:46.78 Allowing 130s for 25.0ml of remaining 525.0ml @13:05:46.79 Intake.select :open @13:05:48.95 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:05:48.96 SS.reconfigure :inhaling 0.76ml/s @13:05:49.01 SS.seek 25ml @13:06:09.61 Vacuum too high -- waiting up to 40 seconds @13:06:09.62 SS.reconfigure :exhale @13:06:09.67 SS.hold @13:06:11.30 Intake @13.8psia, vacuum from 8.3 to 11.3psi @13:06:11.32 SS.reconfigure :inhaling 0.69ml/s @13:06:11.36 SS.seek 25ml @13:06:27.99 Stroke completed -- waiting for pressure to equalize ... @13:06:28.00 SS.reconfigure :exhale @13:06:28.05 SS.hold @13:06:30.94 Intake.select :close @13:06:31.07 SSV.dial :exhaust @13:06:31.81 Exhaust.select :open @13:06:31.92 SS.seek 0ml @13:06:40.95 Exhaust.select :close @13:06:41.05 SSV.dial CBV @13:06:41.10
CBV.dial SSV @13:06:41.81 Allowing 130s for 25.0ml of remaining 500.0ml @13:06:41.82 Intake.select :open @13:06:43.97 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:06:43.99 SS.reconfigure :inhaling 0.69ml/s @13:06:44.03 SS.seek 25ml @13:07:22.88 Stroke completed -- waiting for pressure to equalize ... @13:07:22.89 SS.reconfigure :exhale @13:07:22.94 SS.hold @13:07:27.09 Intake.select :close @13:07:27.22 SSV.dial :exhaust @13:07:27.97 Exhaust.select :open @13:07:28.08 SS.seek 0ml @13:07:37.46 Exhaust.select :close @13:07:37.57 SSV.dial CBV @13:07:37.59
CBV.dial SSV @13:07:38.36 Allowing 130s for 25.0ml of remaining 475.0ml @13:07:38.37 Intake.select :open @13:07:40.52 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:07:40.53 SS.reconfigure :inhaling 0.69ml/s @13:07:40.58 SS.seek 25ml @13:08:17.45 Vacuum too high -- waiting up to 40 seconds @13:08:17.46 SS.reconfigure :exhale @13:08:17.50 SS.hold @13:08:20.49 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:08:20.51 SS.reconfigure :inhaling 0.61ml/s @13:08:20.55 SS.seek 25ml @13:08:22.10 Stroke completed -- waiting for pressure to equalize ... @13:08:22.11 SS.reconfigure :exhale @13:08:22.15 SS.hold @13:08:23.79 Intake.select :close @13:08:23.92 SSV.dial :exhaust @13:08:24.67 Exhaust.select :open @13:08:24.77 SS.seek 0ml @13:08:33.85 Exhaust.select :close @13:08:33.95 SSV.dial CBV @13:08:34.00
CBV.dial SSV @13:08:34.71 Allowing 130s for 25.0ml of remaining 450.0ml @13:08:34.72 Intake.select :open @13:08:36.88 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:08:36.89 SS.reconfigure :inhaling 0.61ml/s @13:08:36.94 SS.seek 25ml @13:09:19.36 Stroke completed -- waiting for pressure to equalize ... @13:09:19.37 SS.reconfigure :exhale @13:09:19.42 SS.hold @13:09:24.29 Intake.select :close @13:09:24.44 SSV.dial :exhaust @13:09:25.18 Exhaust.select :open @13:09:25.29 SS.seek 0ml @13:09:34.65 Exhaust.select :close @13:09:34.75 SSV.dial CBV @13:09:34.77
CBV.dial SSV @13:09:35.52 Allowing 130s for 25.0ml of remaining 425.0ml @13:09:35.53 Intake.select :open @13:09:37.69 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:09:37.70 SS.reconfigure :inhaling 0.61ml/s @13:09:37.75 SS.seek 25ml @13:10:15.95 Vacuum too high -- waiting up to 40 seconds @13:10:15.96 SS.reconfigure :exhale @13:10:16.00 SS.hold @13:10:18.38 Intake @13.3psia, vacuum from 7.8 to 10.8psi @13:10:18.39 SS.reconfigure :inhaling 0.56ml/s @13:10:18.44 SS.seek 25ml @13:10:23.13 Stroke completed -- waiting for pressure to equalize ... @13:10:23.14 SS.reconfigure :exhale @13:10:23.18 SS.hold @13:10:26.15 Intake.select :close @13:10:26.29 SSV.dial :exhaust @13:10:27.04 Exhaust.select :open @13:10:27.14 SS.seek 0ml @13:10:36.47 Exhaust.select :close @13:10:36.58 SSV.dial CBV @13:10:36.61
CBV.dial SSV @13:10:37.34 Allowing 130s for 25.0ml of remaining 400.0ml @13:10:37.35 Intake.select :open @13:10:39.50 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:10:39.51 SS.reconfigure :inhaling 0.56ml/s @13:10:39.56 SS.seek 25ml @13:11:26.13 Stroke completed -- waiting for pressure to equalize ... @13:11:26.14 SS.reconfigure :exhale @13:11:26.18 SS.hold @13:11:32.57 Intake.select :close @13:11:32.71 SSV.dial :exhaust @13:11:33.46 Exhaust.select :open @13:11:33.56 SS.seek 0ml @13:11:42.97 Exhaust.select :close @13:11:43.07 SSV.dial CBV @13:11:43.12
CBV.dial SSV @13:11:43.82 Allowing 130s for 25.0ml of remaining 375.0ml @13:11:43.83 Intake.select :open @13:11:45.98 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:11:45.99 SS.reconfigure :inhaling 0.56ml/s @13:11:46.04 SS.seek 25ml @13:12:17.57 Vacuum too high -- waiting up to 40 seconds @13:12:17.58 SS.reconfigure :exhale @13:12:17.62 SS.hold @13:12:20.10 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:12:20.11 SS.reconfigure :inhaling 0.50ml/s @13:12:20.15 SS.seek 25ml @13:12:37.22 Stroke completed -- waiting for pressure to equalize ... @13:12:37.23 SS.reconfigure :exhale @13:12:37.27 SS.hold @13:12:42.02 Intake.select :close @13:12:42.16 SSV.dial :exhaust @13:12:42.90 Exhaust.select :open @13:12:43.00 SS.seek 0ml @13:12:52.08 Exhaust.select :close @13:12:52.18 SSV.dial CBV @13:12:52.20
CBV.dial SSV @13:12:52.94 Allowing 130s for 25.0ml of remaining 350.0ml @13:12:52.95 Intake.select :open @13:12:55.10 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:12:55.12 SS.reconfigure :inhaling 0.50ml/s @13:12:55.61 SS.seek 25ml @13:13:48.28 Stroke completed -- waiting for pressure to equalize ... @13:13:48.29 SS.reconfigure :exhale @13:13:48.33 SS.hold @13:13:55.70 Intake.select :close @13:13:55.83 SSV.dial :exhaust @13:13:56.58 Exhaust.select :open @13:13:56.68 SS.seek 0ml @13:14:06.28 Exhaust.select :close @13:14:06.38 SSV.dial CBV @13:14:06.40
CBV.dial SSV @13:14:07.14 Allowing 130s for 25.0ml of remaining 325.0ml @13:14:07.15 Intake.select :open @13:14:09.30 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:14:09.32 SS.reconfigure :inhaling 0.50ml/s @13:14:09.36 SS.seek 25ml @13:14:49.85 Vacuum too high -- waiting up to 40 seconds @13:14:49.86 SS.reconfigure :exhale @13:14:49.90 SS.hold @13:14:53.33 Intake @13.9psia, vacuum from 8.4 to 11.4psi @13:14:53.35 SS.reconfigure :inhaling 0.46ml/s @13:14:53.39 SS.seek 25ml @13:15:06.49 Stroke completed -- waiting for pressure to equalize ... @13:15:06.50 SS.reconfigure :exhale @13:15:06.54 SS.hold @13:15:12.16 Intake.select :close @13:15:12.30 SSV.dial :exhaust @13:15:13.04 Exhaust.select :open @13:15:13.15 SS.seek 0ml @13:15:22.23 Exhaust.select :close @13:15:22.33 SSV.dial CBV @13:15:22.36
CBV.dial SSV @13:15:23.09 Allowing 130s for 25.0ml of remaining 300.0ml @13:15:23.10 Intake.select :open @13:15:25.26 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:15:25.27 SS.reconfigure :inhaling 0.46ml/s @13:15:25.32 SS.seek 25ml @13:16:23.49 Stroke completed -- waiting for pressure to equalize ... @13:16:23.50 SS.reconfigure :exhale @13:16:23.54 SS.hold @13:16:31.99 Intake.select :close @13:16:32.12 SSV.dial :exhaust @13:16:32.87 Exhaust.select :open @13:16:32.98 SS.seek 0ml @13:16:42.48 Exhaust.select :close @13:16:42.58 SSV.dial CBV @13:16:42.60
CBV.dial SSV @13:16:43.34 Allowing 130s for 25.0ml of remaining 275.0ml @13:16:43.35 Intake.select :open @13:16:45.50 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:16:45.51 SS.reconfigure :inhaling 0.46ml/s @13:16:45.56 SS.seek 25ml @13:17:43.32 Stroke completed -- waiting for pressure to equalize ... @13:17:43.33 SS.reconfigure :exhale @13:17:43.37 SS.hold @13:17:53.11 Intake.select :close @13:17:53.26 SSV.dial :exhaust @13:17:54.00 Exhaust.select :open @13:17:54.11 SS.seek 0ml @13:18:03.19 Exhaust.select :close @13:18:03.30 SSV.dial CBV @13:18:03.32
CBV.dial SSV @13:18:04.05 Allowing 130s for 25.0ml of remaining 250.0ml @13:18:04.06 Intake.select :open @13:18:06.22 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:18:06.23 SS.reconfigure :inhaling 0.46ml/s @13:18:06.28 SS.seek 25ml @13:18:44.09 Vacuum too high -- waiting up to 40 seconds @13:18:44.10 SS.reconfigure :exhale @13:18:44.14 SS.hold @13:18:48.88 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:18:48.89 SS.reconfigure :inhaling 0.41ml/s @13:18:48.94 SS.seek 25ml @13:19:12.13 Stroke completed -- waiting for pressure to equalize ... @13:19:12.14 SS.reconfigure :exhale @13:19:12.18 SS.hold @13:19:20.70 Intake.select :close @13:19:20.84 SSV.dial :exhaust @13:19:21.58 Exhaust.select :open @13:19:21.69 SS.seek 0ml @13:19:30.69 Exhaust.select :close @13:19:30.79 SSV.dial CBV @13:19:30.84
CBV.dial SSV @13:19:31.55 Allowing 130s for 25.0ml of remaining 225.0ml @13:19:31.56 Intake.select :open @13:19:33.71 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:19:33.73 SS.reconfigure :inhaling 0.41ml/s @13:19:33.77 SS.seek 25ml @13:20:39.75 Stroke completed -- waiting for pressure to equalize ... @13:20:39.76 SS.reconfigure :exhale @13:20:39.80 SS.hold @13:20:50.82 Intake.select :close @13:20:50.95 SSV.dial :exhaust @13:20:51.69 Exhaust.select :open @13:20:51.80 SS.seek 0ml @13:21:00.88 Exhaust.select :close @13:21:00.98 SSV.dial CBV @13:21:01.00
CBV.dial SSV @13:21:01.75 Allowing 130s for 25.0ml of remaining 200.0ml @13:21:01.76 Intake.select :open @13:21:03.91 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:21:03.93 SS.reconfigure :inhaling 0.41ml/s @13:21:03.97 SS.seek 25ml @13:21:59.65 Vacuum too high -- waiting up to 40 seconds @13:21:59.66 SS.reconfigure :exhale @13:21:59.70 SS.hold @13:22:06.17 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:22:06.18 SS.reconfigure :inhaling 0.37ml/s @13:22:06.23 SS.seek 25ml @13:22:18.76 Stroke completed -- waiting for pressure to equalize ... @13:22:18.77 SS.reconfigure :exhale @13:22:18.81 SS.hold @13:22:26.64 Intake.select :close @13:22:26.78 SSV.dial :exhaust @13:22:27.51 Exhaust.select :open @13:22:27.62 SS.seek 0ml @13:22:36.72 Exhaust.select :close @13:22:36.82 SSV.dial CBV @13:22:36.84
CBV.dial SSV @13:22:37.61 Allowing 130s for 25.0ml of remaining 175.0ml @13:22:37.62 Intake.select :open @13:22:39.77 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:22:39.79 SS.reconfigure :inhaling 0.37ml/s @13:22:39.83 SS.seek 25ml @13:23:55.31 Stroke completed -- waiting for pressure to equalize ... @13:23:55.32 SS.reconfigure :exhale @13:23:55.36 SS.hold @13:24:07.89 Intake.select :close @13:24:08.04 SSV.dial :exhaust @13:24:08.78 Exhaust.select :open @13:24:08.88 SS.seek 0ml @13:24:18.48 Exhaust.select :close @13:24:18.58 SSV.dial CBV @13:24:18.61
CBV.dial SSV @13:24:19.36 Allowing 130s for 25.0ml of remaining 150.0ml @13:24:19.37 Intake.select :open @13:24:21.52 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:24:21.54 SS.reconfigure :inhaling 0.37ml/s @13:24:21.58 SS.seek 25ml @13:25:37.90 Stroke completed -- waiting for pressure to equalize ... @13:25:37.91 SS.reconfigure :exhale @13:25:37.95 SS.hold @13:25:52.20 Intake.select :close @13:25:52.34 SSV.dial :exhaust @13:25:53.09 Exhaust.select :open @13:25:53.19 SS.seek 0ml @13:26:02.58 Exhaust.select :close @13:26:02.69 SSV.dial CBV @13:26:02.71
CBV.dial SSV @13:26:03.46 Allowing 130s for 25.0ml of remaining 125.0ml @13:26:03.47 Intake.select :open @13:26:05.63 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:26:05.64 SS.reconfigure :inhaling 0.37ml/s @13:26:05.69 SS.seek 25ml @13:26:53.54 Vacuum too high -- waiting up to 40 seconds @13:26:53.55 SS.reconfigure :exhale @13:26:53.59 SS.hold @13:26:59.21 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:26:59.22 SS.reconfigure :inhaling 0.33ml/s @13:26:59.26 SS.seek 25ml @13:27:32.60 Stroke completed -- waiting for pressure to equalize ... @13:27:32.61 SS.reconfigure :exhale @13:27:32.65 SS.hold @13:27:44.13 Intake.select :close @13:27:44.27 SSV.dial :exhaust @13:27:45.00 Exhaust.select :open @13:27:45.11 SS.seek 0ml @13:27:54.21 Exhaust.select :close @13:27:54.31 SSV.dial CBV @13:27:54.33
CBV.dial SSV @13:27:55.07 Allowing 130s for 25.0ml of remaining 100.0ml @13:27:55.08 Intake.select :open @13:27:57.23 Intake @15.3psia, vacuum from 9.8 to 12.8psi @13:27:57.25 SS.reconfigure :inhaling 0.33ml/s @13:27:57.29 SS.seek 25ml @13:29:27.25 Stroke completed -- waiting for pressure to equalize ... @13:29:27.26 SS.reconfigure :exhale @13:29:27.30 SS.hold @13:29:41.59 Intake.select :close @13:29:41.73 SSV.dial :exhaust @13:29:42.47 Exhaust.select :open @13:29:42.58 SS.seek 0ml @13:29:52.09 Exhaust.select :close @13:29:52.20 SSV.dial CBV @13:29:52.22
CBV.dial SSV @13:29:52.96 Allowing 130s for 25.0ml of remaining 75.0ml @13:29:52.97 Intake.select :open @13:29:55.12 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:29:55.14 SS.reconfigure :inhaling 0.33ml/s @13:29:55.18 SS.seek 25ml @13:31:23.59 Stroke completed -- waiting for pressure to equalize ... @13:31:23.60 SS.reconfigure :exhale @13:31:23.64 SS.hold @13:31:41.85 Intake.select :close @13:31:41.99 SSV.dial :exhaust @13:31:42.73 Exhaust.select :open @13:31:42.84 SS.seek 0ml @13:31:52.34 Exhaust.select :close @13:31:52.44 SSV.dial CBV @13:31:52.46
CBV.dial SSV @13:31:53.23 Allowing 130s for 25.0ml of remaining 50.0ml @13:31:53.24 Intake.select :open @13:31:55.40 Intake @15.4psia, vacuum from 9.9 to 12.9psi @13:31:55.41 SS.reconfigure :inhaling 0.33ml/s @13:31:55.46 SS.seek 25ml @13:33:25.48 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @13:33:25.52 SS.hold @13:33:45.78 Intake.select :close @13:33:45.92 SSV.dial :exhaust @13:33:46.67 Exhaust.select :open @13:33:46.78 SS.seek 0ml @13:33:56.80 Exhaust.select :close @13:33:56.91 SSV.dial CBV @13:33:56.93
CBV.dial SSV @13:33:57.68 Allowing 130s for 24.9ml of remaining 24.9ml @13:33:57.69 Intake.select :open @13:33:59.85 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:33:59.86 SS.reconfigure :inhaling 0.33ml/s @13:33:59.90 SS.seek 24.95ml @13:34:55.84 Vacuum too high -- waiting up to 40 seconds @13:34:55.85 SS.reconfigure :exhale @13:34:55.90 SS.hold @13:35:05.54 Intake @15.6psia, vacuum from 10.0 to 13.0psi @13:35:05.55 SS.reconfigure :inhaling 0.30ml/s @13:35:05.60 SS.seek 24.95ml @13:35:40.96 Stroke completed -- waiting for pressure to equalize ... @13:35:40.97 SS.reconfigure :exhale @13:35:41.01 SS.hold @13:35:57.17 Intake.select :close @13:35:57.31 SSV.dial :exhaust @13:35:58.04 Exhaust.select :open @13:35:58.14 SS.seek 0ml @13:36:07.25 Exhaust.select :close @13:36:07.35 Sampled 1000.0ml @13:36:07.37 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:36:07.38 Intake.select :close @13:36:07.43 CSV.dial :waste @13:36:07.48 CS.seek 0ml @13:36:07.57 CSV.dial :air @13:36:07.60 SSV.dial CBV,avoiding: :exhaust @13:36:07.63
CBV.dial SSV @13:36:08.37 CS.seek 10ml @13:36:09.49
CTV.dial :air @13:36:10.23 delay 15 seconds @13:36:25.25 SSV.dial :spit,avoiding: :exhaust @13:36:26.57 CTV.dial CSV,avoiding: :intake @13:36:26.60
SS.seek 0ml @13:36:26.72 SSV.dial CBV,avoiding: :exhaust @13:36:31.89 CBV.dial between SSV and waste2 @13:36:32.26 CTV.dial CSV,avoiding: :air @13:36:32.28
CSV.dial CTV @13:36:32.84 CS.reconfigure :pressurize @13:36:32.88 CS.seek 0ml @13:36:50.61 BubblePoint: Intake @40.6psia, Outlet @16.3psia @13:36:50.62 CBV.dial SSV @13:36:50.93 SS.reconfigure :default @13:36:50.97 SS.seek 10ml @13:36:56.60 delay 15 seconds @13:37:11.72 Evacuated: Intake @16.3psia, Outlet @10.2psia @13:37:11.73 SSV.dial :spit,avoiding: :exhaust @13:37:13.06 SS.reconfigure :exhale @13:37:13.10 SS.seek 0ml @13:37:18.92 SS.reconfigure :fast @13:37:18.96 SS.seek 5ml @13:37:21.58 SSV.dial CBV,avoiding: :exhaust @13:37:21.61
CBV.dial SSV @13:37:22.93 CTV.dial :air,avoiding: :intake @13:37:24.21 CSV.dial CTV @13:37:24.25
SS.seek 10ml @13:37:25.25 CSV.dial :air @13:37:25.68 CS.reconfigure :fast @13:37:25.72 CS.seek 0ml @13:37:26.77
SSV.dial :spit,avoiding: :exhaust @13:37:28.09 SS.reconfigure :exhale @13:37:28.13 SS.seek 0ml @13:37:33.53 SS.reconfigure :fast @13:37:33.57 SS.seek 5ml @13:37:33.67 CS.stop @13:37:36.39 SSV.dial CBV,avoiding: :exhaust @13:37:36.87
CBV.dial SSV @13:37:39.18 SSV.dial :spit,avoiding: :exhaust @13:37:40.51 SS.reconfigure :default @13:37:40.55 SS.seek 0ml @13:37:43.92 SS.stop @13:37:43.96 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:37:43.97 Intake.select :close @13:37:44.04 CSV.dial :waste @13:37:44.15 SSV.dial CBV,avoiding: :exhaust @13:37:44.17
CBV.dial SSV @13:37:44.82 CS.seek 0ml @13:37:44.89 CSV.dial :air @13:37:45.49
CTV.dial :air @13:37:45.50 delay 15 seconds @13:37:45.75 CS.seek 10ml @13:38:00.52
SSV.dial :spit,avoiding: :exhaust @13:38:01.84 CTV.dial CSV,avoiding: :intake @13:38:01.87
SS.reconfigure :exhale @13:38:01.97 SS.seek 0ml @13:38:02.03 SSV.dial CBV,avoiding: :exhaust @13:38:08.92 CBV.dial between SSV and waste2 @13:38:09.30 CTV.dial CSV,avoiding: :air @13:38:09.32
CSV.dial CTV @13:38:09.78 CS.reconfigure :pressurize @13:38:09.82 CS.seek 0ml @13:38:31.05 BubblePoint: Intake @39.6psia, Outlet @15.5psia @13:38:31.06 CBV.dial SSV @13:38:31.38 SS.reconfigure :default @13:38:31.43 SS.seek 10ml @13:38:36.94 delay 15 seconds @13:38:52.06 Evacuated: Intake @18.9psia, Outlet @12.7psia @13:38:52.07 SSV.dial :spit,avoiding: :exhaust @13:38:53.40 SS.reconfigure :exhale @13:38:53.44 SS.seek 0ml @13:38:58.90 SS.reconfigure :fast @13:38:58.94 SS.seek 5ml @13:39:02.03 SSV.dial CBV,avoiding: :exhaust @13:39:02.06
CBV.dial SSV @13:39:03.36 CTV.dial :air,avoiding: :intake @13:39:04.66 CSV.dial CTV @13:39:04.70
SS.seek 10ml @13:39:05.69 CSV.dial :air @13:39:06.15 CS.reconfigure :fast @13:39:06.19 CS.seek 0ml @13:39:07.47
SSV.dial :spit,avoiding: :exhaust @13:39:08.79 SS.reconfigure :exhale @13:39:08.84 SS.seek 0ml @13:39:11.02 CS.stop @13:39:14.24
SS.reconfigure :fast @13:39:14.28 SS.seek 5ml @13:39:17.05 SSV.dial CBV,avoiding: :exhaust @13:39:17.07
CBV.dial SSV @13:39:19.38 SSV.dial :spit,avoiding: :exhaust @13:39:20.71 SS.reconfigure :default @13:39:20.75 SS.seek 0ml @13:39:24.31 SS.stop @13:39:24.34 Lysing: Adding 2.0ml AqMeOH @13:39:24.36 CBV.dial between CSV and waste1,avoiding: :DBV @13:39:25.83 CTV.dial CSV @13:39:27.13 CSV.dial CSR @13:39:27.88 Pulling 1ml of CSR.AqMeOH @13:39:27.89 CSR.select :AqMeOH @13:39:28.00 CS.reconfigure :default @13:39:28.04 CS.seek 1ml @13:39:31.43 delay 5 seconds @13:39:36.45 CSR.select :air @13:39:36.60 CS.seek 6ml @13:39:50.05 delay 2 seconds @13:39:52.06 CSV.dial :waste2 @13:39:52.49 CS.reconfigure :fast @13:39:52.53 CS.seek 0ml @13:40:06.52 CSV.dial CSR @13:40:07.02 Pulling 2ml of CSR.AqMeOH @13:40:07.03 CSR.select :AqMeOH @13:40:07.14 CS.reconfigure :default @13:40:07.18 CS.seek 2ml @13:40:12.99 delay 5 seconds @13:40:18.01 CSR.select :air @13:40:18.16 CS.seek 5ml @13:40:26.48 delay 2 seconds @13:40:28.50 CSV.dial CTV @13:40:28.93 CS.seek 1ml @13:40:39.93 Before lysing Collection Puck Heater at 11.4C/39.4psia @13:40:39.95 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @13:40:39.96 CH.seek 70C,8:00 @13:43:09.08 CH.hold 10:00,70C @13:43:09.26 Begun lysing Collection Puck Heater at 68C/43.6psia @13:53:09.26 Ended lysing Collection Puck Heater at 70C/44.9psia @13:53:09.27 CS.seek 0ml @13:53:12.75 CTV.dial between spare and air,avoiding: :intake @13:53:13.75 CSV.dial :air @13:53:14.20 CS.seek 5ml @13:53:27.59 CSV.dial CTV @13:53:28.03 delay 2 seconds @13:53:30.04 CSV.dial :air @13:53:30.50 delay 2 seconds @13:53:32.51 CSV.dial CTV @13:53:32.97 delay 2 seconds @13:53:34.99 CSV.dial :air @13:53:35.43 delay 2 seconds @13:53:37.46 CS.seek 0ml @13:53:50.95 Puck @72.4psia after final lysis pressurization @13:53:50.96 Recovering Lysate into Collection syringe @13:53:50.97 CSV.dial CBV @13:53:51.98 CBV.dial CSV @13:53:52.34 CS.seek 5ml @13:54:05.78 delay 5 seconds @13:54:10.79 CTV.dial :air @13:54:11.29 CS.seek 7ml @13:54:17.12 delay 5 seconds @13:54:22.14 CSV.dial :air2 @13:54:22.58 CS.seek 10ml @13:54:31.03 CSV.dial between air and CTV,avoiding: CBV @13:54:32.16 Lysing Complete @13:54:32.22 Storing sh1 puck in Collection Clamp to tube 2 @13:54:32.38 SC.reconfigure :tight @13:54:32.42 SC.seek 2 @13:54:35.97 SC.configure :limp @13:54:36.16 Elbow.reconfigure :tight @13:54:36.20 Elbow.seek 2 @13:54:38.03 Forearm.reconfigure :tight @13:54:38.07 Forearm.seek 2 + 700 counts @13:54:39.20 SE.reconfigure :pick @13:54:39.25 SE.seek :beamBreak @13:54:40.83 SE.configure :default @13:54:40.99 Elbow.reconfigure :limp @13:54:41.04 Forearm.reconfigure :loose @13:54:41.08 Forearm.seek 2 @13:54:41.48 Elbow.hold @13:54:41.54 Elbow.reconfigure :tight @13:54:41.63 SE.seek 30.38 Pucks below Top Plate @13:54:42.12 Forearm.force -20 @13:54:42.16 Forearm.seek 2 + 301 counts @13:54:42.47 Forearm.reconfigure :tight @13:54:42.52 Forearm.seek 2 + 700 counts @13:54:42.72 SE.seek 31.38 Pucks below Top Plate @13:54:43.35 Forearm.reconfigure :loose @13:54:43.39 Forearm.seek :retract @13:54:44.31 Elbow.seek CC @13:54:45.93 Elbow.reconfigure :loose @13:54:46.01 Forearm.seek CC @13:54:47.03 Hand.close @13:54:47.72 CC.seek :open @13:54:52.15 Forearm.seek :retract @13:54:53.16 Elbow.reconfigure :tight @13:54:53.20 Elbow.seek 2 @13:54:54.88 Elbow.reconfigure :limp @13:54:54.93 Forearm.seek 2 @13:54:56.05 Elbow.hold @13:54:56.11 Elbow.reconfigure :tight @13:54:56.15 Hand.open @13:54:56.96 Forearm.force -20 @13:54:56.99 Forearm.seek 2 + 319 counts @13:54:57.28 Forearm.reconfigure :tight @13:54:57.32 Forearm.seek 2 + 700 counts @13:54:57.67 SE.seek :down @13:54:58.26 SE.stop @13:54:58.80
Forearm.reconfigure :loose @13:54:58.84 Forearm.seek :retract @13:54:59.75 Tranferring DA @13:54:59.76 Beginning samplePassC @13:54:59.77 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:54:59.95 Elbow.seek FlushPuck::Garage @13:55:01.83 Elbow.reconfigure :loose @13:55:01.92 Forearm.seek :garage @13:55:03.04 Hand.close @13:55:03.78 Forearm.seek :retract @13:55:04.87 Elbow.reconfigure :tight @13:55:04.92 Elbow.seek CC @13:55:07.82 Elbow.reconfigure :loose @13:55:07.90 Forearm.reconfigure :tight @13:55:07.94 Forearm.seek CC @13:55:09.23 CC.seek :closed @13:55:13.45 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.85 @13:55:13.52 Hand.open @13:55:14.27 Forearm.reconfigure :loose @13:55:14.31 Forearm.seek :retract @13:55:15.32 PSV.dial PSR @13:55:16.66 Pulling 1.5ml of PSR.flush @13:55:16.67 PSR.select :flush @13:55:16.79 PS.seek 1.5ml @13:55:32.86 delay 5 seconds @13:55:37.88 PSV.dial :waste1 @13:55:38.32 PS.seek 0ml @13:55:54.36 PSV.dial PSR @13:55:54.80 PSR.select :air @13:55:54.91 PS.seek 2.5ml @13:56:21.05 PRV.dial CBV,avoiding: :ab2 @13:56:21.55
CBV.dial PRV @13:56:23.20 PSV.dial :mixloop @13:56:24.22 CTV.dial :air,avoiding: :intake @13:56:24.23 PTV.dial :PRVmixing @13:56:25.49 PS.seek 0ml @13:56:51.67 PSV.dial :air @13:56:52.10 PS.seek 2.5ml @13:57:18.45 PSV.dial :mixloop @13:57:18.90 PS.seek 0ml @13:57:45.04 PSV.dial :air @13:57:45.49 PS.seek 2.5ml @13:58:11.70 PSV.dial :mixloop @13:58:12.14 PS.seek 0ml @13:58:38.39 Garaging FlushPuck in Collection Clamp @13:58:38.46 Elbow.reconfigure :tight @13:58:38.50 Elbow.seek CC @13:58:38.73 Elbow.reconfigure :loose @13:58:38.82 Forearm.seek CC @13:58:39.86 Hand.close @13:58:40.46 CC.seek :open @13:58:44.82 Forearm.seek :retract @13:58:45.82 Elbow.reconfigure :tight @13:58:45.86 Elbow.seek :garage @13:58:48.73 Elbow.reconfigure :loose @13:58:48.82 Forearm.reconfigure :tight @13:58:48.86 Forearm.seek :garage @13:58:49.96 Hand.open @13:58:50.77 Forearm.reconfigure :loose @13:58:50.81 Forearm.seek :retract @13:58:51.83 PSV.dial :mixloop @13:58:51.84 PTV.dial :PRVmixing @13:58:51.86 PRV.dial CBV @13:58:51.88
CBV.dial PRV @13:58:51.90 CTV.dial :air,avoiding: :intake @13:58:51.91 Loading lyfil puck from tube 3 into Collection Clamp @13:58:52.13 SC.reconfigure :tight @13:58:52.17 SC.seek 3 @13:58:55.74 SC.configure :limp @13:58:55.93 Elbow.reconfigure :tight @13:58:55.97 Elbow.seek 3 @13:58:57.96 Forearm.reconfigure :tight @13:58:58.00 Forearm.seek 3 + 700 counts @13:58:58.97 SE.reconfigure :pick @13:58:59.02 SE.seek :beamBreak @13:59:02.03 SE.configure :default @13:59:02.17 Elbow.seek 3 @13:59:02.44 Forearm.seek 3 + 300 counts @13:59:02.69 Forearm.reconfigure :loose @13:59:02.73 Hand.close @13:59:03.35 SE.seek :down @13:59:03.53
Forearm.seek :retract @13:59:04.46 Elbow.seek CC @13:59:06.10 SE.stop @13:59:06.14
Elbow.reconfigure :loose @13:59:06.25 Forearm.reconfigure :tight @13:59:06.29 Forearm.seek CC @13:59:07.58 CC.seek :closed @13:59:11.82 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.61 @13:59:11.83 Hand.open @13:59:12.65 Forearm.reconfigure :loose @13:59:12.69 Forearm.seek :retract @13:59:13.68 CTV.dial CSV,avoiding: :intake @13:59:14.97 CSV.dial CTV @13:59:15.74 PS.seek 2.5ml @13:59:42.19 CS.seek 5.5ml @13:59:54.33 delay 8 seconds @14:00:02.35 PSV.dial :air @14:00:02.79 delay 2 seconds @14:00:04.81 PSV.dial :waste2 @14:00:05.55 PS.seek 0ml @14:00:31.79 CBV.dial between PRV and waste1 @14:00:32.13 CSV.dial :air @14:00:32.59 CS.seek 10ml @14:00:44.73 CSV.dial CTV @14:00:45.18 CS.seek 0ml @14:01:11.45 CBV.dial :waste1 @14:01:11.75 delay 5 seconds @14:01:16.77 CTV.dial :air,avoiding: :intake @14:01:18.10 Storing lyfil puck in Collection Clamp to tube 2 @14:01:18.26 SC.reconfigure :tight @14:01:18.30 SC.seek 2 @14:01:21.87 SC.configure :limp @14:01:22.06 Elbow.reconfigure :tight @14:01:22.10 Elbow.seek 2 @14:01:23.84 Forearm.reconfigure :tight @14:01:23.89 Forearm.seek 2 + 700 counts @14:01:24.87 SE.reconfigure :pick @14:01:24.92 SE.seek :beamBreak @14:01:25.95 SE.configure :default @14:01:26.09 Elbow.reconfigure :limp @14:01:26.14 Forearm.reconfigure :loose @14:01:26.17 Forearm.seek 2 @14:01:26.58 Elbow.hold @14:01:26.64 Elbow.reconfigure :tight @14:01:26.73 SE.seek 31.38 Pucks below Top Plate @14:01:27.27 Forearm.force -20 @14:01:27.30 Forearm.seek 2 + 314 counts @14:01:27.61 Forearm.reconfigure :tight @14:01:27.66 Forearm.seek 2 + 700 counts @14:01:27.94 SE.seek 32.38 Pucks below Top Plate @14:01:28.58 Forearm.reconfigure :loose @14:01:28.62 Forearm.seek :retract @14:01:29.53 Elbow.seek CC @14:01:31.19 Elbow.reconfigure :loose @14:01:31.28 Forearm.seek CC @14:01:32.30 Hand.close @14:01:33.08 CC.seek :open @14:01:37.51 Forearm.seek :retract @14:01:38.52 Elbow.reconfigure :tight @14:01:38.56 Elbow.seek 2 @14:01:40.31 Elbow.reconfigure :limp @14:01:40.35 Forearm.seek 2 @14:01:41.48 Elbow.hold @14:01:41.55 Elbow.reconfigure :tight @14:01:41.60 Hand.open @14:01:42.45 Forearm.force -20 @14:01:42.48 Forearm.seek 2 + 319 counts @14:01:42.82 Forearm.reconfigure :tight @14:01:42.86 Forearm.seek 2 + 700 counts @14:01:43.14 SE.seek :down @14:01:43.65 SE.stop @14:01:44.28
Forearm.reconfigure :loose @14:01:44.32 Forearm.seek :retract @14:01:45.25 PSV.dial :air @14:01:45.98 PS.seek 2.5ml @14:02:12.16 delay 1 second @14:02:13.17 PSV.dial :mixloop @14:02:13.62 delay 1 second @14:02:14.63 PSV.dial :air @14:02:15.07 delay 1 second @14:02:16.08 PSV.dial :mixloop @14:02:16.52 delay 1 second @14:02:17.54 PSV.dial :air @14:02:18.43 delay 1 second @14:02:19.45 PS.seek 0ml @14:02:45.66 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:02:45.83 Elbow.seek FlushPuck::Garage @14:02:47.79 Elbow.reconfigure :loose @14:02:47.87 Forearm.seek :garage @14:02:48.98 Hand.close @14:02:49.69 Forearm.seek :retract @14:02:50.76 Elbow.reconfigure :tight @14:02:50.80 Elbow.seek PC @14:02:52.15 Elbow.reconfigure :loose @14:02:52.24 Forearm.reconfigure :tight @14:02:52.28 Forearm.seek PC @14:02:53.61 PC.seek :closed @14:02:57.47 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.37 @14:02:57.53 Hand.open @14:02:58.26 Forearm.reconfigure :loose @14:02:58.30 Forearm.seek :retract @14:02:59.32 PRV.dial :air @14:02:59.77 PSV.dial :mixloop @14:03:00.27 PS.seek 0.3ml @14:03:04.07 PSV.dial PSR @14:03:05.10 Pulling 0.4ml of PSR.air @14:03:05.11 PSR.select :air @14:03:05.14 PS.seek 0.7ml @14:03:10.04 delay 3 seconds @14:03:13.05 PSR.select :air @14:03:13.07 PS.seek 2.5ml @14:03:32.10 delay 3 seconds @14:03:35.11 PSV.dial :mixloop @14:03:36.10 PTV.dial :mixing @14:03:36.80 PBV.dial :waste2 @14:03:37.69 PS.seek 0ml @14:04:03.94 Garaging FlushPuck in Processing Clamp @14:04:04.01 Elbow.reconfigure :tight @14:04:04.05 Elbow.seek PC @14:04:04.16 Elbow.reconfigure :loose @14:04:04.24 Forearm.seek PC @14:04:05.31 Hand.close @14:04:05.95 PC.seek :open @14:04:09.89 Forearm.seek :retract @14:04:10.92 Elbow.reconfigure :tight @14:04:10.96 Elbow.seek :garage @14:04:12.35 Elbow.reconfigure :loose @14:04:12.44 Forearm.reconfigure :tight @14:04:12.48 Forearm.seek :garage @14:04:13.57 Hand.open @14:04:14.38 Forearm.reconfigure :loose @14:04:14.42 Forearm.seek :retract @14:04:15.50 Loading da puck from tube 4 into Processing Clamp @14:04:15.71 SC.reconfigure :tight @14:04:15.76 SC.seek 4 @14:04:22.47 SC.configure :limp @14:04:22.66 Elbow.reconfigure :tight @14:04:22.70 Elbow.seek 4 @14:04:24.59 Forearm.reconfigure :tight @14:04:24.63 Forearm.seek 4 + 700 counts @14:04:25.76 SE.reconfigure :pick @14:04:25.81 SE.seek :beamBreak @14:04:26.74 SE.configure :default @14:04:26.88 Elbow.seek 4 @14:04:27.05 Forearm.seek 4 + 300 counts @14:04:27.35 Forearm.reconfigure :loose @14:04:27.39 Hand.close @14:04:27.96 SE.seek :down @14:04:28.16
Forearm.seek :retract @14:04:29.08 SE.stop @14:04:29.17
Elbow.seek PC @14:04:31.78 Elbow.reconfigure :loose @14:04:31.87 Forearm.reconfigure :tight @14:04:31.92 Forearm.seek PC @14:04:33.27 PC.seek :closed @14:04:37.17 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.33 @14:04:37.18 Hand.open @14:04:38.00 Forearm.reconfigure :loose @14:04:38.66 Forearm.seek :retract @14:04:39.66 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @14:04:39.68 PH.seek 28C,5:00 @14:05:30.02 PH.hold 2:00:00,28C @14:05:30.08 delay 1:30 @14:07:00.10 PSR.select :air @14:07:00.11 PSV.dial PSR @14:07:01.18 Pulling 0.5ml of PSR.wash @14:07:01.19 PSR.select :wash @14:07:01.30 PS.seek 0.5ml @14:07:07.16 delay 5 seconds @14:07:12.17 PSR.select :air @14:07:12.29 PS.seek 2ml @14:07:28.37 PTV.dial :puck @14:07:29.09 PSV.dial PTV @14:07:29.83 PBV.dial :waste1 @14:07:31.15 PS.seek 1.5ml @14:07:37.09 delay 3 seconds @14:07:40.11 PBV.dial between PSV and waste1 @14:07:40.48 PS.seek 0ml @14:07:56.53 delay 5:00 @14:12:56.55 recoverPSRreagent08 @14:12:56.56 PTV.dial :puck @14:12:56.57 PBV.dial :waste1 @14:12:56.94 delay 30 seconds @14:13:26.97 PBV.dial between PSV and waste1 @14:13:27.32 PSR.select :air @14:13:27.33 PSV.dial PSR @14:13:28.07 PS.reconfigure :fast @14:13:28.12 PS.seek 2.5ml @14:13:51.14 PSV.dial PTV @14:13:51.86 delay 5 seconds @14:13:56.93 PSV.dial PSR @14:13:57.66 delay 5 seconds @14:14:02.68 PSV.dial PTV @14:14:03.41 delay 5 seconds @14:14:08.43 PSV.dial :air @14:14:09.15 PS.seek 0ml @14:14:31.86 PTV.dial :PRVmixing,via: PRV @14:14:33.11 PSV.dial :mixloop @14:14:33.55 PRV.dial :ab1 @14:14:34.29 PS.reconfigure :slow @14:14:34.33 PS.seek 0.15ml @14:14:37.83 delay 2 seconds @14:14:39.85 CBV.dial PRV @14:14:39.87
PRV.dial CBV @14:14:40.44 PS.seek 0.34ml @14:14:44.77 delay 2 seconds @14:14:46.78 CBV.dial between PRV and PSV @14:14:47.09 PRV.dial :air @14:14:47.54 PS.seek 0.9ml @14:14:59.36 PRV.dial :waste2 @14:15:00.09 PS.seek 0.5ml @14:15:08.64 addPMLReagent08 @14:15:08.65 PSV.dial :air @14:15:09.10 PS.reconfigure :default @14:15:09.14 PS.seek 2ml @14:15:25.11 PSV.dial :PML @14:15:26.03 PTV.dial :mixing @14:15:26.75 PBV.dial :waste1 @14:15:27.16 PS.seek 1.85ml @14:15:29.49 PBV.dial between PSV and waste1 @14:15:29.85 PS.seek 0ml @14:15:49.45 delay 2 seconds @14:15:51.47 Beginning samplePassC @14:15:51.48 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:15:51.65 Elbow.reconfigure :tight @14:15:51.69 Elbow.seek FlushPuck::Garage @14:15:53.09 Elbow.reconfigure :loose @14:15:53.18 Forearm.seek :garage @14:15:54.33 Hand.close @14:15:55.16 Forearm.seek :retract @14:15:56.23 Elbow.reconfigure :tight @14:15:56.27 Elbow.seek CC @14:15:59.11 Elbow.reconfigure :loose @14:15:59.20 Forearm.reconfigure :tight @14:15:59.24 Forearm.seek CC @14:16:00.48 CC.seek :closed @14:16:04.71 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.86 @14:16:04.78 Hand.open @14:16:05.61 Forearm.reconfigure :loose @14:16:05.65 Forearm.seek :retract @14:16:06.64 PSV.dial PSR @14:16:07.71 Pulling 1.5ml of PSR.flush @14:16:07.72 PSR.select :flush @14:16:07.83 PS.seek 1.5ml @14:16:23.86 delay 5 seconds @14:16:28.88 PSV.dial :waste1 @14:16:29.44 PS.seek 0ml @14:16:45.48 PSV.dial PSR @14:16:45.91 PSR.select :air @14:16:46.02 PS.seek 2.5ml @14:17:12.24 PRV.dial CBV,avoiding: :ab2 @14:17:12.26
CBV.dial PRV @14:17:13.84 PSV.dial :mixloop @14:17:14.95 CTV.dial :air,avoiding: :intake @14:17:14.97 PTV.dial :PRVmixing @14:17:15.68 PS.seek 0ml @14:17:41.89 PSV.dial :air @14:17:42.35 PS.seek 2.5ml @14:18:08.48 PSV.dial :mixloop @14:18:08.93 PS.seek 0ml @14:18:35.07 PSV.dial :air @14:18:35.52 PS.seek 2.5ml @14:19:01.67 PSV.dial :mixloop @14:19:02.11 PS.seek 0ml @14:19:28.23 Garaging FlushPuck in Collection Clamp @14:19:28.30 Elbow.reconfigure :tight @14:19:28.34 Elbow.seek CC @14:19:28.58 Elbow.reconfigure :loose @14:19:28.67 Forearm.seek CC @14:19:29.65 Hand.close @14:19:30.42 CC.seek :open @14:19:34.75 Forearm.seek :retract @14:19:35.77 Elbow.reconfigure :tight @14:19:35.81 Elbow.seek :garage @14:19:38.69 Elbow.reconfigure :loose @14:19:38.78 Forearm.reconfigure :tight @14:19:38.82 Forearm.seek :garage @14:19:40.00 Hand.open @14:19:40.85 Forearm.reconfigure :loose @14:19:40.89 Forearm.seek :retract @14:19:41.94 delayUntil "14:23:51.46" @14:19:41.96 Whole Cell Archive sampling 1000ml @14:19:41.98 Loading wcr puck from tube 4 into Collection Clamp @14:19:42.66 SC.reconfigure :tight @14:19:42.70 SC.seek 4 @14:19:42.76 SC.configure :limp @14:19:42.97 Elbow.reconfigure :tight @14:19:43.02 Elbow.seek 4 @14:19:45.01 Forearm.reconfigure :tight @14:19:45.06 Forearm.seek 4 + 700 counts @14:19:46.04 SE.reconfigure :pick @14:19:46.10 SE.seek :beamBreak @14:19:47.18 SE.configure :default @14:19:47.32 Elbow.seek 4 @14:19:47.51 Forearm.seek 4 + 300 counts @14:19:47.75 Forearm.reconfigure :loose @14:19:47.79 Hand.close @14:19:48.56 SE.seek :down @14:19:48.75 Forearm.seek :retract @14:19:49.22 SE.stop @14:19:49.72 Elbow.seek CC @14:19:51.23 Elbow.reconfigure :loose @14:19:51.33 Forearm.reconfigure :tight @14:19:51.37 Forearm.seek CC @14:19:52.69 CC.seek :closed @14:19:56.93 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.73 @14:19:56.94 Hand.open @14:19:57.73 Forearm.reconfigure :loose @14:19:57.77 Forearm.seek :retract @14:19:58.72 collectSample 1000ml w/bubble point of 35psi @14:19:58.73 Priming 7ml @14:19:58.74 Intake.select :close @14:19:58.75 Exhaust.select :close @14:19:58.76 SS.hold @14:19:58.96 Calibrating pressure sensors @14:19:58.97 CTV.dial :air @14:19:58.98 SSV.dial :spit,avoiding: :exhaust @14:19:59.00 SS.reconfigure :fast @14:19:59.05 SS.seek 5ml @14:20:02.01 SSV.dial CBV @14:20:02.04 CBV.dial SSV @14:20:03.36 delay 3.8 seconds @14:20:07.29 SSV.dial :spit @14:20:08.60 SS.seek 0ml @14:20:11.24 SSV.dial CBV @14:20:13.50 CTV.dial :intake @14:20:14.24 SSV.dial CBV @14:20:14.26 CBV.dial SSV @14:20:14.28 Allowing 40s for 7.0ml of remaining 7.0ml @14:20:14.29 Intake.select :open @14:20:16.45 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:20:16.47 SS.reconfigure :inhaling 2.14ml/s @14:20:16.51 SS.seek 7ml @14:20:23.11 Stroke completed -- waiting for pressure to equalize ... @14:20:23.12 SS.reconfigure :exhale @14:20:23.16 SS.hold @14:20:41.48 Intake.select :close @14:20:41.63 SSV.dial :exhaust @14:20:42.38 Exhaust.select :open @14:20:42.49 SS.seek 0ml @14:20:47.06 Exhaust.select :close @14:20:47.17 Sampling 1000.0ml @14:20:47.18 Intake.select :close @14:20:47.19 Exhaust.select :close @14:20:47.20 SS.reconfigure :default @14:20:47.24 SS.hold @14:20:47.44 CTV.dial :intake @14:20:47.46 SSV.dial CBV @14:20:47.48 CBV.dial SSV @14:20:48.24 Allowing 130s for 25.0ml of remaining 1000.0ml @14:20:48.25 Intake.select :open @14:20:50.40 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:20:50.42 SS.reconfigure :inhaling 2.14ml/s @14:20:50.47 SS.seek 25ml @14:20:54.32 Vacuum too high -- waiting up to 40 seconds @14:20:54.33 SS.reconfigure :exhale @14:20:54.38 SS.hold @14:20:57.28 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:20:57.30 SS.reconfigure :inhaling 1.93ml/s @14:20:57.34 SS.seek 25ml @14:20:59.61 Vacuum too high -- waiting up to 40 seconds @14:20:59.62 SS.reconfigure :exhale @14:20:59.66 SS.hold @14:21:01.91 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:21:01.93 SS.reconfigure :inhaling 1.74ml/s @14:21:01.97 SS.seek 25ml @14:21:04.07 Vacuum too high -- waiting up to 40 seconds @14:21:04.08 SS.reconfigure :exhale @14:21:04.12 SS.hold @14:21:05.93 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:21:05.95 SS.reconfigure :inhaling 1.57ml/s @14:21:05.99 SS.seek 25ml @14:21:07.94 Vacuum too high -- waiting up to 40 seconds @14:21:07.96 SS.reconfigure :exhale @14:21:08.00 SS.hold @14:21:09.77 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:21:09.78 SS.reconfigure :inhaling 1.41ml/s @14:21:09.83 SS.seek 25ml @14:21:11.85 Vacuum too high -- waiting up to 40 seconds @14:21:11.87 SS.reconfigure :exhale @14:21:11.91 SS.hold @14:21:13.63 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:21:13.65 SS.reconfigure :inhaling 1.27ml/s @14:21:13.69 SS.seek 25ml @14:21:15.76 Vacuum too high -- waiting up to 40 seconds @14:21:15.77 SS.reconfigure :exhale @14:21:15.81 SS.hold @14:21:17.16 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:21:17.18 SS.reconfigure :inhaling 1.14ml/s @14:21:17.22 SS.seek 25ml @14:21:19.32 Vacuum too high -- waiting up to 40 seconds @14:21:19.33 SS.reconfigure :exhale @14:21:19.38 SS.hold @14:21:21.05 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:21:21.07 SS.reconfigure :inhaling 1.03ml/s @14:21:21.11 SS.seek 25ml @14:21:23.32 Vacuum too high -- waiting up to 40 seconds @14:21:23.33 SS.reconfigure :exhale @14:21:23.37 SS.hold @14:21:24.78 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:21:24.80 SS.reconfigure :inhaling 0.93ml/s @14:21:24.84 SS.seek 25ml @14:21:27.67 Vacuum too high -- waiting up to 40 seconds @14:21:27.68 SS.reconfigure :exhale @14:21:27.72 SS.hold @14:21:29.12 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:21:29.13 SS.reconfigure :inhaling 0.84ml/s @14:21:29.18 SS.seek 25ml @14:21:32.54 Vacuum too high -- waiting up to 40 seconds @14:21:32.55 SS.reconfigure :exhale @14:21:32.59 SS.hold @14:21:34.24 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:21:34.26 SS.reconfigure :inhaling 0.76ml/s @14:21:34.30 SS.seek 25ml @14:21:37.77 Stroke completed -- waiting for pressure to equalize ... @14:21:37.78 SS.reconfigure :exhale @14:21:37.82 SS.hold @14:21:40.72 Intake.select :close @14:21:40.87 SSV.dial :exhaust @14:21:41.59 Exhaust.select :open @14:21:41.70 SS.seek 0ml @14:21:50.76 Exhaust.select :close @14:21:50.87 SSV.dial CBV @14:21:50.89 CBV.dial SSV @14:21:51.64 Allowing 130s for 25.0ml of remaining 975.0ml @14:21:51.65 Intake.select :open @14:21:53.81 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:21:53.83 SS.reconfigure :inhaling 0.76ml/s @14:21:53.87 SS.seek 25ml @14:21:59.97 Vacuum too high -- waiting up to 40 seconds @14:21:59.99 SS.reconfigure :exhale @14:22:00.03 SS.hold @14:22:00.99 Intake @14.2psia, vacuum from 8.7 to 11.7psi @14:22:01.01 SS.reconfigure :inhaling 0.69ml/s @14:22:01.05 SS.seek 25ml @14:22:10.50 Vacuum too high -- waiting up to 40 seconds @14:22:10.52 SS.reconfigure :exhale @14:22:10.56 SS.hold @14:22:12.61 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:22:12.62 SS.reconfigure :inhaling 0.61ml/s @14:22:12.67 SS.seek 25ml @14:22:25.16 Vacuum too high -- waiting up to 40 seconds @14:22:25.17 SS.reconfigure :exhale @14:22:25.21 SS.hold @14:22:26.87 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:22:26.89 SS.reconfigure :inhaling 0.56ml/s @14:22:26.93 SS.seek 25ml @14:22:42.39 Stroke completed -- waiting for pressure to equalize ... @14:22:42.40 SS.reconfigure :exhale @14:22:42.44 SS.hold @14:22:46.60 Intake.select :close @14:22:46.74 SSV.dial :exhaust @14:22:47.49 Exhaust.select :open @14:22:47.60 SS.seek 0ml @14:22:56.61 Exhaust.select :close @14:22:56.71 SSV.dial CBV @14:22:56.73 CBV.dial SSV @14:22:57.48 Allowing 130s for 25.0ml of remaining 950.0ml @14:22:57.50 Intake.select :open @14:23:00.13 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:23:00.15 SS.reconfigure :inhaling 0.56ml/s @14:23:00.19 SS.seek 25ml @14:23:19.60 Vacuum too high -- waiting up to 40 seconds @14:23:19.61 SS.reconfigure :exhale @14:23:19.66 SS.hold @14:23:20.94 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:23:20.96 SS.reconfigure :inhaling 0.50ml/s @14:23:21.01 SS.seek 25ml @14:23:51.47 recoverPRVreagent08 @14:23:51.49 PTV.dial :mixing @14:23:52.21 PBV.dial :waste1 @14:23:52.42 Stroke completed -- waiting for pressure to equalize ... @14:23:52.43 SS.reconfigure :exhale @14:23:52.47 SS.hold @14:23:52.58 delay 30 seconds @14:23:56.55 Intake.select :close @14:23:56.70 SSV.dial :exhaust @14:23:57.45 Exhaust.select :open @14:23:57.56 SS.seek 0ml @14:24:06.63 Exhaust.select :close @14:24:06.74 SSV.dial CBV @14:24:06.76 CBV.dial SSV @14:24:07.51 Allowing 130s for 25.0ml of remaining 925.0ml @14:24:07.52 Intake.select :open @14:24:09.68 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:24:09.70 SS.reconfigure :inhaling 0.50ml/s @14:24:09.74 SS.seek 25ml @14:24:22.61 PBV.dial between PSV and waste1 @14:24:22.97 PSV.dial :air @14:24:23.43 PS.reconfigure :fast @14:24:23.47 PS.seek 2.5ml @14:24:44.50 Vacuum too high -- waiting up to 40 seconds @14:24:44.51 SS.reconfigure :exhale @14:24:44.56 SS.hold @14:24:46.22 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:24:46.24 SS.reconfigure :inhaling 0.46ml/s @14:24:46.28 SS.seek 25ml @14:24:46.60 PSV.dial :mixloop @14:24:47.05 delay 2 seconds @14:24:49.07 PSV.dial :air @14:24:49.54 delay 2 seconds @14:24:51.56 PSV.dial :mixloop @14:24:52.02 delay 2 seconds @14:24:54.04 PSV.dial :air @14:24:54.51 PS.seek 0ml @14:25:05.37 Stroke completed -- waiting for pressure to equalize ... @14:25:05.38 SS.reconfigure :exhale @14:25:05.42 SS.hold @14:25:09.52 Intake.select :close @14:25:09.66 SSV.dial :exhaust @14:25:10.41 Exhaust.select :open @14:25:10.52 SS.seek 0ml @14:25:17.53 washCycleDA #1 of 2 @14:25:17.54 PSR.select :air @14:25:17.56 PSV.dial PSR @14:25:19.02 Pulling 0.5ml of PSR.wash @14:25:19.03 PSR.select :wash @14:25:19.15 PS.reconfigure :default @14:25:19.19 PS.seek 0.5ml @14:25:19.62 Exhaust.select :close @14:25:19.72 SSV.dial CBV @14:25:19.76 CBV.dial SSV @14:25:20.49 Allowing 130s for 25.0ml of remaining 900.0ml @14:25:20.51 Intake.select :open @14:25:22.67 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:25:22.68 SS.reconfigure :inhaling 0.46ml/s @14:25:22.73 SS.seek 25ml @14:25:25.04 delay 5 seconds @14:25:30.05 PSR.select :air @14:25:30.16 PS.seek 2ml @14:25:46.19 PTV.dial :puck @14:25:46.91 PSV.dial PTV @14:25:47.66 PBV.dial :waste1 @14:25:48.09 PS.seek 1.5ml @14:25:54.50 delay 3 seconds @14:25:57.52 PBV.dial between PSV and waste1 @14:25:57.90 PS.seek 0ml @14:26:13.91 delay 2:00 @14:26:20.57 Stroke completed -- waiting for pressure to equalize ... @14:26:20.58 SS.reconfigure :exhale @14:26:20.62 SS.hold @14:26:26.17 Intake.select :close @14:26:26.32 SSV.dial :exhaust @14:26:27.07 Exhaust.select :open @14:26:27.18 SS.seek 0ml @14:26:36.21 Exhaust.select :close @14:26:36.32 SSV.dial CBV @14:26:36.34 CBV.dial SSV @14:26:37.10 Allowing 130s for 25.0ml of remaining 875.0ml @14:26:37.11 Intake.select :open @14:26:39.27 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:26:39.29 SS.reconfigure :inhaling 0.46ml/s @14:26:39.33 SS.seek 25ml @14:27:19.18 Vacuum too high -- waiting up to 40 seconds @14:27:19.20 SS.reconfigure :exhale @14:27:19.24 SS.hold @14:27:22.24 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:27:22.26 SS.reconfigure :inhaling 0.41ml/s @14:27:22.30 SS.seek 25ml @14:27:42.36 Stroke completed -- waiting for pressure to equalize ... @14:27:42.37 SS.reconfigure :exhale @14:27:42.41 SS.hold @14:27:47.96 Intake.select :close @14:27:48.10 SSV.dial :exhaust @14:27:48.85 Exhaust.select :open @14:27:48.96 SS.seek 0ml @14:27:58.00 Exhaust.select :close @14:27:58.11 SSV.dial CBV @14:27:58.13 CBV.dial SSV @14:27:58.86 Allowing 130s for 25.0ml of remaining 850.0ml @14:27:58.88 Intake.select :open @14:28:01.03 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:28:01.05 SS.reconfigure :inhaling 0.41ml/s @14:28:01.09 SS.seek 25ml @14:28:13.93 recoverPSRreagent08 @14:28:13.95 PTV.dial :puck @14:28:13.96 PBV.dial :waste1 @14:28:14.33 delay 30 seconds @14:28:44.35 PBV.dial between PSV and waste1 @14:28:44.70 PSR.select :air @14:28:44.72 PSV.dial PSR @14:28:45.58 PS.reconfigure :fast @14:28:45.62 PS.seek 2.5ml @14:29:05.80 Stroke completed -- waiting for pressure to equalize ... @14:29:05.81 SS.reconfigure :exhale @14:29:05.85 SS.hold @14:29:08.63 PSV.dial PTV @14:29:09.37 delay 5 seconds @14:29:14.38 Intake.select :close @14:29:14.44 PSV.dial PSR @14:29:14.53 SSV.dial :exhaust @14:29:15.17 delay 5 seconds @14:29:15.77 Exhaust.select :open @14:29:15.88 SS.seek 0ml @14:29:20.18 PSV.dial PTV @14:29:20.92 delay 5 seconds @14:29:25.43 Exhaust.select :close @14:29:25.54 SSV.dial CBV @14:29:25.57 CBV.dial SSV @14:29:25.93 PSV.dial :air @14:29:26.32 Allowing 130s for 25.0ml of remaining 825.0ml @14:29:26.33 Intake.select :open @14:29:26.68 PS.seek 0ml @14:29:28.48 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:29:28.50 SS.reconfigure :inhaling 0.41ml/s @14:29:28.55 SS.seek 25ml @14:29:49.43 washCycleDA #2 of 2 @14:29:49.44 PSR.select :air @14:29:49.46 PSV.dial PSR @14:29:50.92 Pulling 0.5ml of PSR.wash @14:29:50.93 PSR.select :wash @14:29:51.05 PS.reconfigure :default @14:29:51.09 PS.seek 0.5ml @14:29:57.00 delay 5 seconds @14:29:59.43 Vacuum too high -- waiting up to 40 seconds @14:29:59.44 SS.reconfigure :exhale @14:29:59.49 SS.hold @14:30:02.01 PSR.select :air @14:30:02.12 PS.seek 2ml @14:30:02.38 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:30:02.40 SS.reconfigure :inhaling 0.37ml/s @14:30:02.44 SS.seek 25ml @14:30:18.09 PTV.dial :puck @14:30:18.10 PSV.dial PTV @14:30:18.84 PBV.dial :waste1 @14:30:19.27 PS.seek 1.5ml @14:30:25.15 delay 3 seconds @14:30:28.17 PBV.dial between PSV and waste1 @14:30:28.55 PS.seek 0ml @14:30:33.68 Vacuum too high -- waiting up to 40 seconds @14:30:33.69 SS.reconfigure :exhale @14:30:33.73 SS.hold @14:30:37.34 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:30:37.36 SS.reconfigure :inhaling 0.33ml/s @14:30:37.40 SS.seek 25ml @14:30:44.53 delay 2:00 @14:30:44.57 Stroke completed -- waiting for pressure to equalize ... @14:30:44.58 SS.reconfigure :exhale @14:30:44.62 SS.hold @14:30:49.49 Intake.select :close @14:30:49.65 SSV.dial :exhaust @14:30:50.39 Exhaust.select :open @14:30:50.50 SS.seek 0ml @14:31:00.06 Exhaust.select :close @14:31:00.17 SSV.dial CBV @14:31:00.19 CBV.dial SSV @14:31:00.96 Allowing 130s for 25.0ml of remaining 800.0ml @14:31:00.97 Intake.select :open @14:31:03.12 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:31:03.14 SS.reconfigure :inhaling 0.33ml/s @14:31:03.19 SS.seek 25ml @14:32:27.31 Stroke completed -- waiting for pressure to equalize ... @14:32:27.32 SS.reconfigure :exhale @14:32:27.36 SS.hold @14:32:36.40 Intake.select :close @14:32:36.54 SSV.dial :exhaust @14:32:37.28 Exhaust.select :open @14:32:37.39 SS.seek 0ml @14:32:44.55 recoverPSRreagent08 @14:32:44.56 PTV.dial :puck @14:32:44.57 PBV.dial :waste1 @14:32:44.94 delay 30 seconds @14:32:46.85 Exhaust.select :close @14:32:46.96 SSV.dial CBV @14:32:46.99 CBV.dial SSV @14:32:47.73 Allowing 130s for 25.0ml of remaining 775.0ml @14:32:47.74 Intake.select :open @14:32:49.91 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:32:49.93 SS.reconfigure :inhaling 0.33ml/s @14:32:49.97 SS.seek 25ml @14:33:14.96 PBV.dial between PSV and waste1 @14:33:15.32 PSR.select :air @14:33:15.34 PSV.dial PSR @14:33:16.20 PS.reconfigure :fast @14:33:16.24 PS.seek 2.5ml @14:33:39.15 PSV.dial PTV @14:33:39.87 delay 5 seconds @14:33:44.88 PSV.dial PSR @14:33:45.62 delay 5 seconds @14:33:50.63 PSV.dial PTV @14:33:51.37 delay 5 seconds @14:33:56.38 PSV.dial :air @14:33:57.12 PS.seek 0ml @14:33:59.23 Vacuum too high -- waiting up to 40 seconds @14:33:59.25 SS.reconfigure :exhale @14:33:59.29 SS.hold @14:34:04.06 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:34:04.07 SS.reconfigure :inhaling 0.30ml/s @14:34:04.12 SS.seek 25ml @14:34:19.96 getPMLReagent08 @14:34:19.97 PRV.dial :air @14:34:20.41 delay 5 seconds @14:34:21.27 Stroke completed -- waiting for pressure to equalize ... @14:34:21.28 SS.reconfigure :exhale @14:34:21.32 SS.hold @14:34:25.43 PTV.dial :PRVmixing @14:34:26.69 delay 5 seconds @14:34:29.77 Intake.select :close @14:34:29.92 SSV.dial :exhaust @14:34:30.67 Exhaust.select :open @14:34:30.78 SS.seek 0ml @14:34:31.71 PRV.dial :ab2 @14:34:32.16 PTV.dial :PRVmixing @14:34:32.17 PSV.dial :mixloop @14:34:32.62 PS.reconfigure :default @14:34:32.66 PS.seek 0.3ml @14:34:36.55 delay 2 seconds @14:34:38.57 PRV.dial :air @14:34:39.06 PS.seek 0.55ml @14:34:40.33 Exhaust.select :close @14:34:40.44 SSV.dial CBV @14:34:40.47 CBV.dial SSV @14:34:41.21 Allowing 130s for 25.0ml of remaining 750.0ml @14:34:41.22 Intake.select :open @14:34:42.43 addPMLReagent08 @14:34:42.44 PSV.dial :air @14:34:42.88 PS.seek 2ml @14:34:43.38 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:34:43.40 SS.reconfigure :inhaling 0.30ml/s @14:34:43.44 SS.seek 25ml @14:34:58.45 PSV.dial :PML @14:34:58.91 PTV.dial :mixing @14:34:59.64 PBV.dial :waste1 @14:35:00.05 PS.seek 1.85ml @14:35:02.33 PBV.dial between PSV and waste1 @14:35:02.70 PS.seek 0ml @14:35:22.36 delay 2 seconds @14:35:24.38 delay 8:00 @14:35:58.04 Vacuum too high -- waiting up to 40 seconds @14:35:58.07 SS.reconfigure :exhale @14:35:58.12 SS.hold @14:36:02.78 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:36:02.80 SS.reconfigure :inhaling 0.27ml/s @14:36:02.84 SS.seek 25ml @14:36:26.52 Stroke completed -- waiting for pressure to equalize ... @14:36:26.53 SS.reconfigure :exhale @14:36:26.57 SS.hold @14:36:37.61 Intake.select :close @14:36:37.75 SSV.dial :exhaust @14:36:38.49 Exhaust.select :open @14:36:38.60 SS.seek 0ml @14:36:47.74 Exhaust.select :close @14:36:47.84 SSV.dial CBV @14:36:47.88 CBV.dial SSV @14:36:48.61 Allowing 130s for 25.0ml of remaining 725.0ml @14:36:48.63 Intake.select :open @14:36:50.78 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:36:50.80 SS.reconfigure :inhaling 0.27ml/s @14:36:50.84 SS.seek 25ml @14:38:37.93 Stroke completed -- waiting for pressure to equalize ... @14:38:37.95 SS.reconfigure :exhale @14:38:37.99 SS.hold @14:38:56.35 Intake.select :close @14:38:56.53 SSV.dial :exhaust @14:38:57.28 Exhaust.select :open @14:38:57.39 SS.seek 0ml @14:39:07.03 Exhaust.select :close @14:39:07.14 SSV.dial CBV @14:39:07.16 CBV.dial SSV @14:39:07.92 Allowing 130s for 25.0ml of remaining 700.0ml @14:39:07.93 Intake.select :open @14:39:10.09 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:39:10.11 SS.reconfigure :inhaling 0.27ml/s @14:39:10.15 SS.seek 25ml @14:40:17.97 Vacuum too high -- waiting up to 40 seconds @14:40:17.98 SS.reconfigure :exhale @14:40:18.03 SS.hold @14:40:27.78 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:40:27.79 SS.reconfigure :inhaling 0.24ml/s @14:40:27.84 SS.seek 25ml @14:41:02.57 Vacuum too high -- waiting up to 40 seconds @14:41:02.58 SS.reconfigure :exhale @14:41:02.62 SS.hold @14:41:09.49 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:41:09.51 SS.reconfigure :inhaling 0.21ml/s @14:41:09.55 SS.seek 25ml @14:41:17.96 SS.reconfigure :default @14:41:18.00 SS.hold @14:41:18.16 Intake.select :close @14:41:18.31 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 324.5ml @14:41:18.32 SSV.dial :exhaust @14:41:19.06 Exhaust.select :open @14:41:19.17 SS.reconfigure :exhale @14:41:19.21 SS.seek 0ml @14:41:28.15 Exhaust.select :close @14:41:28.26 Sampled 324.5ml @14:41:28.27 Drying puck before fixing for 20:00 @14:41:28.28 CBV.dial :waste2b @14:41:29.02 delay 1:00 @14:42:29.04 CSV.dial :air @14:42:29.49 CS.reconfigure :fast @14:42:29.54 CS.seek 10ml @14:42:52.67 Pumping wcr puck from tube 4 up to 35psi... @14:42:52.69 CBV.dial SSV @14:42:53.97 CBV.dial between CSV and waste2b @14:42:54.90 CSV.dial between air and CTV @14:42:55.23 CS.seek 7.287ml @14:43:02.18 CTV.dial CSV,avoiding: :air @14:43:02.20 CSV.dial CTV @14:43:03.10 CS.reconfigure :pressurize @14:43:03.15 CS.seek 0ml @14:43:16.57 CBV.dial :waste2b @14:43:16.89 CS.reconfigure :default @14:43:16.94 CS.seek 0ml @14:43:23.11 delay 1:00 @14:43:24.40 recoverPRVreagent08 @14:43:24.41 PTV.dial :mixing @14:43:24.42 PBV.dial :waste1 @14:43:24.77 delay 30 seconds @14:43:54.80 PBV.dial between PSV and waste1 @14:43:55.16 PSV.dial :air @14:43:55.62 PS.reconfigure :fast @14:43:55.66 PS.seek 2.5ml @14:44:18.66 PSV.dial :mixloop @14:44:19.10 delay 2 seconds @14:44:21.11 PSV.dial :air @14:44:21.57 delay 2 seconds @14:44:23.13 CTV.dial :air,avoiding: :intake @14:44:23.58 PSV.dial :mixloop @14:44:24.04 delay 2 seconds @14:44:24.43 Fix #1 of 2 (with RNAlater) for 10:00 @14:44:24.44 CSV.dial CSR @14:44:24.93 Pulling 2ml of CSR.RNAlater @14:44:24.94 CSR.select :RNAlater @14:44:25.05 CS.seek 2ml @14:44:26.06 PSV.dial :air @14:44:26.51 PS.seek 0ml @14:44:30.95 delay 5 seconds @14:44:35.97 CSR.select :air @14:44:36.08 CS.reconfigure :fast @14:44:36.13 CS.seek 10ml @14:44:49.35 washCycleDA #1 of 3 @14:44:49.36 PSR.select :air @14:44:49.38 PSV.dial PSR @14:44:50.82 Pulling 0.5ml of PSR.wash @14:44:50.83 PSR.select :wash @14:44:50.94 PS.reconfigure :default @14:44:50.99 PS.seek 0.5ml @14:44:54.80 Pumping wcr puck from tube 4 up to 35psi... @14:44:54.82 CBV.dial SSV @14:44:56.09 CBV.dial between CSV and waste2b @14:44:57.04 delay 5 seconds @14:44:57.06 CSV.dial between air and CTV @14:44:57.66 CS.seek 7.129ml @14:45:02.06 PSR.select :air @14:45:02.17 PS.seek 2ml @14:45:04.92 CTV.dial CSV,avoiding: :air @14:45:05.41 CSV.dial CTV @14:45:06.78 CS.reconfigure :pressurize @14:45:06.83 CS.seek 0ml @14:45:18.16 PTV.dial :puck @14:45:18.89 PSV.dial PTV @14:45:19.64 PBV.dial :waste1 @14:45:20.08 PS.seek 1.5ml @14:45:23.35 delay 10:00 @14:45:26.03 delay 3 seconds @14:45:29.05 PBV.dial between PSV and waste1 @14:45:29.43 PS.seek 0ml @14:45:45.47 delay 2:00 @14:47:45.50 recoverPSRreagent08 @14:47:45.51 PTV.dial :puck @14:47:45.52 PBV.dial :waste1 @14:47:45.89 delay 30 seconds @14:48:15.91 PBV.dial between PSV and waste1 @14:48:16.28 PSR.select :air @14:48:16.30 PSV.dial PSR @14:48:17.16 PS.reconfigure :fast @14:48:17.20 PS.seek 2.5ml @14:48:40.40 PSV.dial PTV @14:48:41.12 delay 5 seconds @14:48:46.14 PSV.dial PSR @14:48:46.87 delay 5 seconds @14:48:51.89 PSV.dial PTV @14:48:52.62 delay 5 seconds @14:48:57.64 PSV.dial :air @14:48:58.36 PS.seek 0ml @14:49:21.15 washCycleDA #2 of 3 @14:49:21.16 PSR.select :air @14:49:21.18 PSV.dial PSR @14:49:22.50 Pulling 0.5ml of PSR.wash @14:49:22.51 PSR.select :wash @14:49:22.62 PS.reconfigure :default @14:49:22.67 PS.seek 0.5ml @14:49:28.59 delay 5 seconds @14:49:33.61 PSR.select :air @14:49:33.73 PS.seek 2ml @14:49:49.74 PTV.dial :puck @14:49:49.76 PSV.dial PTV @14:49:50.48 PBV.dial :waste1 @14:49:50.91 PS.seek 1.5ml @14:49:56.74 delay 3 seconds @14:49:59.76 PBV.dial between PSV and waste1 @14:50:00.13 PS.seek 0ml @14:50:16.15 delay 2:00 @14:52:16.18 recoverPSRreagent08 @14:52:16.19 PTV.dial :puck @14:52:16.20 PBV.dial :waste1 @14:52:16.57 delay 30 seconds @14:52:46.60 PBV.dial between PSV and waste1 @14:52:46.94 PSR.select :air @14:52:46.96 PSV.dial PSR @14:52:47.70 PS.reconfigure :fast @14:52:47.74 PS.seek 2.5ml @14:53:10.74 PSV.dial PTV @14:53:11.48 delay 5 seconds @14:53:16.49 PSV.dial PSR @14:53:17.22 delay 5 seconds @14:53:22.24 PSV.dial PTV @14:53:22.97 delay 5 seconds @14:53:27.99 PSV.dial :air @14:53:28.72 PS.seek 0ml @14:53:51.46 washCycleDA #3 of 3 @14:53:51.47 PSR.select :air @14:53:51.48 PSV.dial PSR @14:53:52.93 Pulling 0.5ml of PSR.wash @14:53:52.94 PSR.select :wash @14:53:53.06 PS.reconfigure :default @14:53:53.10 PS.seek 0.5ml @14:53:58.96 delay 5 seconds @14:54:03.98 PSR.select :air @14:54:04.09 PS.seek 2ml @14:54:20.14 PTV.dial :puck @14:54:20.16 PSV.dial PTV @14:54:20.90 PBV.dial :waste1 @14:54:21.33 PS.seek 1.5ml @14:54:27.18 delay 3 seconds @14:54:30.19 PBV.dial between PSV and waste1 @14:54:30.57 PS.seek 0ml @14:54:46.80 delay 2:00 @14:55:23.37 CBV.dial :waste2b @14:55:23.70 CS.reconfigure :default @14:55:23.75 CS.seek 0ml @14:55:26.37 delay 1:00 @14:56:26.40 CTV.dial :air,avoiding: :intake @14:56:27.70 Fix #2 of 2 (with RNAlater) for 10:00 @14:56:27.72 CSV.dial CSR @14:56:28.22 Pulling 1.999ml of CSR.RNAlater @14:56:28.23 CSR.select :RNAlater @14:56:28.34 CS.seek 2ml @14:56:34.31 delay 5 seconds @14:56:39.33 CSR.select :air @14:56:39.44 CS.reconfigure :fast @14:56:39.48 CS.seek 10ml @14:56:46.82 recoverPSRreagent08 @14:56:46.83 PTV.dial :puck @14:56:46.85 PBV.dial :waste1 @14:56:47.20 delay 30 seconds @14:56:58.28 Pumping wcr puck from tube 4 up to 35psi... @14:56:58.29 CBV.dial SSV @14:56:59.58 CBV.dial between CSV and waste2b @14:57:00.52 CSV.dial between air and CTV @14:57:01.13 CS.seek 7.036ml @14:57:08.56 CTV.dial CSV,avoiding: :air @14:57:08.58 CSV.dial CTV @14:57:09.94 CS.reconfigure :pressurize @14:57:09.98 CS.seek 0ml @14:57:17.22 PBV.dial between PSV and waste1 @14:57:17.59 PSR.select :air @14:57:17.61 PSV.dial PSR @14:57:18.35 PS.reconfigure :fast @14:57:18.39 PS.seek 2.5ml @14:57:26.55 delay 10:00 @14:57:41.36 PSV.dial PTV @14:57:42.08 delay 5 seconds @14:57:47.10 PSV.dial PSR @14:57:47.83 delay 5 seconds @14:57:52.85 PSV.dial PTV @14:57:53.58 delay 5 seconds @14:57:58.60 PSV.dial :air @14:57:59.33 PS.seek 0ml @14:58:22.23 Gate.power :camera,:ON @14:58:22.24 Mixing DA Substrates @14:58:22.25 PSR.select :air @14:58:22.27 PSV.dial PSR @14:58:23.87 Pulling 0.25ml of PSR.sub1 @14:58:23.92 PSR.select :sub1 @14:58:24.18 PS.reconfigure :default @14:58:24.39 PS.seek 0.25ml @14:58:27.87 delay 5 seconds @14:58:32.94 Pulling 0.25ml of PSR.sub2 @14:58:32.95 PSR.select :sub2 @14:58:33.06 PS.seek 0.5ml @14:58:36.61 delay 5 seconds @14:58:41.63 PSR.select :air @14:58:41.73 PS.seek 2ml @14:58:57.76 PTV.dial :puck @14:58:57.77 PSV.dial PTV @14:58:58.50 PBV.dial :waste1 @14:58:58.93 PS.seek 1.5ml @14:59:04.73 delay 3 seconds @14:59:07.75 PBV.dial between PSV and waste1 @14:59:08.13 PS.seek 0ml @14:59:24.17 delay 10 seconds @14:59:34.19 recoverPSRreagent08 @14:59:34.20 PTV.dial :puck @14:59:34.21 PBV.dial :waste1 @14:59:34.58 delay 30 seconds @15:00:04.60 PBV.dial between PSV and waste1 @15:00:04.96 PSR.select :air @15:00:04.98 PSV.dial PSR @15:00:05.74 PS.reconfigure :fast @15:00:05.78 PS.seek 2.5ml @15:00:29.10 PSV.dial PTV @15:00:29.84 delay 5 seconds @15:00:34.86 PSV.dial PSR @15:00:35.59 delay 5 seconds @15:00:40.61 PSV.dial PTV @15:00:41.34 delay 5 seconds @15:00:46.36 PSV.dial :air @15:00:47.09 PS.seek 0ml @15:01:10.23 Additional air clear @15:01:10.24 PSR.select :air @15:01:10.26 PSV.dial PSR @15:01:11.59 Pulling 2ml of PSR.air @15:01:11.60 PSR.select :air @15:01:11.63 PS.reconfigure :default @15:01:11.67 PS.seek 2ml @15:01:32.73 delay 5 seconds @15:01:37.75 PTV.dial :puck @15:01:37.76 PSV.dial PTV @15:01:38.49 PBV.dial :waste1 @15:01:38.91 PS.seek 1.5ml @15:01:44.88 delay 3 seconds @15:01:47.90 PBV.dial between PSV and waste1 @15:01:48.28 delay 1 second @15:01:49.29 PSV.dial :waste1 @15:01:50.33 PS.seek 0ml @15:02:06.46 Processing Puck Heater at 27.9C before imaging @15:02:06.47 PH.coast @15:02:06.60 Imaging da puck in Processing Clamp while storing it to tube 3 @15:02:06.75 SC.reconfigure :tight @15:02:06.80 SC.seek 3 @15:02:10.38 SC.configure :limp @15:02:10.50 SE.reconfigure :place @15:02:10.63 SE.seek :beamBreak @15:02:10.69 Elbow.reconfigure :tight @15:02:10.74 Elbow.seek PC @15:02:13.45 SE.configure :default @15:02:13.49 SE.seek :place @15:02:14.44 Elbow.reconfigure :loose @15:02:14.54 Forearm.seek PC @15:02:15.64 Hand.close @15:02:16.34 PC.seek :open @15:02:20.89 Forearm.seek :retract @15:02:21.92 Elbow.reconfigure :tight @15:02:21.97 Elbow.seek 3 @15:02:24.67 Forearm.reconfigure :tight @15:02:24.72 Forearm.seek 3 + 700 counts @15:02:25.72 Hand.open @15:02:26.53 SE.seek -0.17 Pucks below Top Plate @15:02:26.87 SE.reconfigure :seal @15:02:26.91 SE.seek -1.28 Pucks below Top Plate @15:02:27.52 Lights, camera, ACTION! @15:02:27.56 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23mar3015h1000ml.tif @15:02:46.97 Waiting >20 seconds for da to relinquish FlushPuck @15:03:12.27 SX MX5/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 41.253 seconds /var/log/esp/da23mar3015h1000ml.tif: TIFF Upload Complete @15:03:12.30 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23mar3015h1000ml15s.tif @15:03:28.03 SX MX5/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23mar3015h1000ml15s.tif: TIFF Upload Complete @15:03:28.06 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da23mar3015h1000ml25s.tif @15:03:53.79 SX MX5/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da23mar3015h1000ml25s.tif: TIFF Upload Complete @15:03:53.80 Gate.power :camera,:OFF @15:03:53.82 DAfans.email "41.253 sec auto-exposure",Subject: "da23mar3015h1000ml images!" @15:03:54.13 Elbow.seek 3 @15:03:54.32 Forearm.seek 3 + 700 counts @15:03:54.40 SE.reconfigure :gently @15:03:54.47 SE.seek -0.56 Pucks below Top Plate @15:03:55.12 Elbow.reconfigure :limp @15:03:55.17 Forearm.reconfigure :loose @15:03:55.21 Forearm.seek 3 @15:03:55.73 Elbow.hold @15:03:55.80 Elbow.reconfigure :tight @15:03:55.85 SE.reconfigure :default @15:03:55.91 SE.seek -0.38 Pucks below Top Plate @15:03:56.29 Forearm.force -20 @15:03:56.33 Forearm.seek 3 + 308 counts @15:03:56.67 Forearm.reconfigure :tight @15:03:56.71 Forearm.seek 3 + 700 counts @15:03:57.21 SE.seek :down @15:03:58.46 Forearm.reconfigure :loose @15:03:58.55 Forearm.seek :retract @15:03:59.99 SE.stop @15:04:00.18 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:04:00.54 Elbow.seek FlushPuck::Garage @15:04:02.47 Elbow.reconfigure :loose @15:04:02.68 Forearm.seek :garage @15:04:03.89 Hand.close @15:04:04.75 Forearm.seek :retract @15:04:05.87 Elbow.reconfigure :tight @15:04:05.96 Elbow.seek PC @15:04:07.40 Elbow.reconfigure :loose @15:04:07.62 Forearm.reconfigure :tight @15:04:07.71 Forearm.seek PC @15:04:09.35 PC.seek :closed @15:04:13.44 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.40 @15:04:13.59 Hand.open @15:04:14.55 Forearm.reconfigure :loose @15:04:14.63 Forearm.seek :retract @15:04:15.70 PSR.select :air @15:04:15.74 PSV.dial PSR @15:04:17.35 Pulling 0.5ml of PSR.flush @15:04:17.37 PSR.select :flush @15:04:17.54 PS.seek 0.5ml @15:04:23.50 delay 5 seconds @15:04:27.94 upsync @15:04:28.54 PSR.select :air @15:04:28.66 PS.seek 2.5ml @15:04:49.72 delay 1 second @15:04:50.74 PRV.dial :waste2,avoiding: :ab2 @15:04:52.57 PTV.dial :PRVmixing @15:04:53.82 PSV.dial :mixloop @15:04:54.83 PS.seek :empty @15:05:20.97 PSR.select :air @15:05:20.99 PSV.dial PSR @15:05:22.06 Pulling 0.5ml of PSR.flush @15:05:22.07 PSR.select :flush @15:05:22.18 PS.seek 0.5ml @15:05:28.04 delay 5 seconds @15:05:33.06 PSR.select :air @15:05:33.17 PS.seek 2.5ml @15:05:54.25 delay 1 second @15:05:55.27 PBV.dial :waste1 @15:05:55.63 PTV.dial :mixing @15:05:56.35 PSV.dial :mixloop @15:05:57.36 PS.seek :empty @15:06:23.59 PSV.dial PSR @15:06:24.72 PSR.select :flush @15:06:24.84 PS.seek 1ml @15:06:35.87 PSR.select :air @15:06:35.99 PS.seek 2.5ml @15:06:52.06 PSV.dial PTV @15:06:52.78 PTV.dial :puck @15:06:53.50 PBV.dial :waste1 @15:06:53.52 PS.seek 0ml @15:07:19.66 PSV.dial PSR @15:07:20.39 PSR.select :flush @15:07:20.51 PS.seek 0.5ml @15:07:26.43 PSR.select :air @15:07:26.55 PS.seek 2.5ml @15:07:26.59 CBV.dial :waste2b @15:07:26.90 CS.reconfigure :default @15:07:26.95 CS.seek 0ml @15:07:29.26 delay 1:00 @15:07:47.62 PSV.dial PTV @15:07:48.34 PBV.dial PSV @15:07:48.90 PS.seek 0ml @15:08:15.03 PSV.dial PBV @15:08:15.49 PS.seek 2.5ml @15:08:29.28 CTV.dial :air,avoiding: :intake @15:08:30.61 Storing wcr puck in Collection Clamp to tube 3 @15:08:30.98 Elbow.reconfigure :tight @15:08:31.03 Elbow.seek 3 @15:08:33.72 Forearm.reconfigure :tight @15:08:33.77 Forearm.seek 3 + 700 counts @15:08:34.72 SE.reconfigure :pick @15:08:34.78 SE.seek :beamBreak @15:08:37.81 SE.configure :default @15:08:37.97 Elbow.reconfigure :limp @15:08:38.01 Forearm.reconfigure :loose @15:08:38.06 Forearm.seek 3 @15:08:38.44 Elbow.hold @15:08:38.50 Elbow.reconfigure :tight @15:08:38.59 SE.seek 0.18 Pucks below Top Plate @15:08:39.02 Forearm.force -20 @15:08:39.05 Forearm.seek 3 + 319 counts @15:08:39.38 Forearm.reconfigure :tight @15:08:39.43 Forearm.seek 3 + 700 counts @15:08:39.75 SE.seek 1.18 Pucks below Top Plate @15:08:40.23 Forearm.reconfigure :loose @15:08:40.27 Forearm.seek :retract @15:08:41.19 Elbow.seek CC @15:08:41.62 PSV.dial :waste1 @15:08:42.35 PS.seek 0ml @15:08:42.74 Elbow.reconfigure :loose @15:08:43.28 Forearm.seek CC @15:08:44.25 Hand.close @15:08:44.96 CC.seek :open @15:08:49.41 Forearm.seek :retract @15:08:50.41 Elbow.reconfigure :tight @15:08:50.46 Elbow.seek 3 @15:08:52.17 Elbow.reconfigure :limp @15:08:52.22 Forearm.seek 3 @15:08:53.36 Elbow.hold @15:08:53.43 Elbow.reconfigure :tight @15:08:53.47 Hand.open @15:08:54.32 Forearm.force -20 @15:08:54.36 Forearm.seek 3 + 320 counts @15:08:54.68 Forearm.reconfigure :tight @15:08:54.73 Forearm.seek 3 + 700 counts @15:08:55.01 SE.seek :down @15:08:56.13 Forearm.reconfigure :loose @15:08:56.18 Forearm.seek :retract @15:08:57.60 SE.stop @15:08:57.64
Starting finalPurge @15:09:08.53 PSV.dial PSR @15:09:09.09 PSR.select :air @15:09:09.12 PS.seek 2.5ml @15:09:17.36
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:09:35.34 PSV.dial PTV @15:09:36.07 PBV.dial PSV @15:09:36.09 PS.seek 0ml @15:10:02.34 PSV.dial PBV @15:10:02.80 PS.seek 2.5ml @15:10:28.93 PSV.dial :waste1 @15:10:29.66 PS.seek 0ml @15:10:55.77 PSV.dial PSR @15:10:56.34 PSR.select :flush @15:10:56.45 PS.seek 1ml @15:11:07.40 PSR.select :air @15:11:07.51 PS.seek 2.5ml @15:11:23.55 PSV.dial PTV @15:11:24.29 PTV.dial :puck @15:11:24.30 PBV.dial :waste1 @15:11:24.84 PS.seek 0ml @15:11:51.02 PSV.dial PSR @15:11:51.76 PSR.select :flush @15:11:51.87 PS.seek 0.5ml @15:11:57.74 PSR.select :air @15:11:57.85 PS.seek 2.5ml @15:12:18.96 PSV.dial PTV @15:12:19.69 PBV.dial PSV @15:12:20.25 PS.seek 0ml @15:12:46.36 PSV.dial PBV @15:12:46.83 PS.seek 2.5ml @15:13:13.05 PSV.dial :waste1 @15:13:13.76 PS.seek 0ml @15:13:39.96 PSV.dial PSR @15:13:40.52 PSR.select :air @15:13:40.54 PS.seek 2.5ml @15:14:06.77 PSV.dial PTV @15:14:07.50 PBV.dial PSV @15:14:07.52 PS.seek 0ml @15:14:33.71 PSV.dial PBV @15:14:34.17 PS.seek 2.5ml @15:15:00.33 PSV.dial :waste1 @15:15:01.06 PS.seek 0ml @15:15:27.21
Loading FlushPuck into Collection Clamp from Processing Clamp @15:15:27.38 Elbow.seek PC @15:15:29.85 Elbow.reconfigure :loose @15:15:29.94 Forearm.seek PC @15:15:31.00 Hand.close @15:15:31.57 PC.seek :open @15:15:35.54 Forearm.seek :retract @15:15:36.57 Elbow.reconfigure :tight @15:15:36.61 Elbow.seek CC @15:15:40.21 Elbow.reconfigure :loose @15:15:40.30 Forearm.reconfigure :tight @15:15:40.34 Forearm.seek CC @15:15:41.54 CC.seek :closed @15:15:45.76 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.76 @15:15:45.83 Hand.open @15:15:46.63 Forearm.reconfigure :loose @15:15:46.67 Forearm.seek :retract @15:15:47.63 Flushing the Collection Valve::Manifold @15:15:47.75 CSV.dial CSR @15:15:48.24 Pulling 5ml of CSR.flush @15:15:48.25 CSR.select :flush @15:15:48.37 CS.seek 5ml @15:16:01.75 delay 5 seconds @15:16:06.76 CSR.select :air @15:16:06.91 CS.reconfigure :fast @15:16:06.96 CS.seek 7.5ml @15:16:13.37 CSV.dial :waste @15:16:14.64 CS.reconfigure :default @15:16:14.68 CS.seek 0ml @15:16:34.46 Flushing lower manifold's waste1 port @15:16:34.58 CSV.dial :flush @15:16:35.01 CS.reconfigure :fast @15:16:35.06 CS.seek 5ml @15:16:46.96 delay 5 seconds @15:16:51.98 CSV.dial :air @15:16:52.48 CS.seek 7ml @15:16:57.71 CSV.dial CTV @15:16:57.76
CTV.dial CSV @15:16:59.07 CBV.dial :waste1 @15:16:59.81 CS.seek 0ml @15:17:15.87 Flushing lower manifold's waste2 port @15:17:15.98 CSV.dial :flush @15:17:16.72 CS.seek 5ml @15:17:28.65 delay 5 seconds @15:17:33.67 CSV.dial :air @15:17:34.18 CS.seek 7ml @15:17:39.46 CSV.dial CTV @15:17:39.49
CTV.dial CSV @15:17:39.93 CBV.dial :waste2 @15:17:40.95 CS.seek 0ml @15:17:57.12 Flushing lower manifold's waste2b port @15:17:57.23 CSV.dial :flush @15:17:57.97 CS.seek 5ml @15:18:09.77 delay 5 seconds @15:18:14.79 CSV.dial :air @15:18:15.30 CS.seek 7ml @15:18:20.55 CSV.dial CTV @15:18:20.58
CTV.dial CSV @15:18:21.01 CBV.dial :waste2b @15:18:22.03 CS.seek 0ml @15:18:38.12 Disinfecting external Intake with kill @15:18:38.23 CSV.dial CSR @15:18:38.72 Pulling 8ml of CSR.kill CSR.select :kill @15:18:38.83 CS.seek 8ml @15:18:57.21 delay 5 seconds @15:19:02.23 CSR.select :air @15:19:02.34 CS.seek 10ml @15:19:07.56 CSV.dial CBV @15:19:07.58
CBV.dial CSV @15:19:08.63 CTV.dial :intake @15:19:09.33 Intake.select :open @15:19:09.44 CS.seek 2ml @15:19:27.64 Intake.select :close @15:19:27.74 CTV.dial :air @15:19:28.49 CS.reconfigure :default @15:19:28.54 CS.seek 0ml @15:19:34.36 CSV.dial CSR @15:19:35.42 Pulling 5ml of CSR.flush @15:19:35.43 CSR.select :flush @15:19:35.54 CS.seek 5ml @15:19:48.99 delay 5 seconds @15:19:54.01 CSR.select :air @15:19:54.11 CS.seek 10ml @15:20:07.58 CSV.dial CBV @15:20:08.57 CS.seek 0ml @15:20:34.58 Garaging FlushPuck in Collection Clamp @15:20:34.65 Elbow.reconfigure :tight @15:20:34.69 Elbow.seek CC @15:20:34.79 Elbow.reconfigure :loose @15:20:34.88 Forearm.seek CC @15:20:35.84 Hand.close @15:20:36.89 CC.seek :open @15:20:41.22 Forearm.seek :retract @15:20:42.24 Elbow.reconfigure :tight @15:20:42.29 Elbow.seek :garage @15:20:45.23 Elbow.reconfigure :loose @15:20:45.32 Forearm.reconfigure :tight @15:20:45.37 Forearm.seek :garage @15:20:46.51 Hand.open @15:20:47.36 Forearm.reconfigure :loose @15:20:47.41 Forearm.seek :retract @15:20:48.46 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 12:46:43 @15:20:48.51 Asleep after completing DA_WCR_033023 assays @15:20:48.52 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 2,\n 4 => 31,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:15:48, 17.7C, 55% humidity, 15.5psia, 13.919V, 0.386A, 0.380A avg, 5.37W\nSampled 324.5ml",Subject: "Asleep after completing DA_WCR_033023 assays" @15:20:52.53 upsync @15:23:03.17UTC06-Apr-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @15:23:05.80 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @15:23:09.75 Polling Can every 10:00 @15:23:09.91 Cleanly exited at 12:59:16UTC06-Apr-23 @15:23:09.95 Loading /home/esp/esp2/mission/DA_WCR_040623.rb mission @15:23:09.99 GoodNews.email "Starting DA_WCR_040623 w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 2,\n 4 => 31,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:23:09, 17.5C, 56% humidity, 15.5psia, 13.977V, 0.257A, 0.251A avg, 3.59W",Subject: "DA_WCR_040623 begun" @15:23:10.04
Preparing to sleep until + 00:00:05 @15:23:10.06 Asleep til + 00:00:05 @15:23:10.08 Continuing at 15:23 @15:23:10.36 Gate.power :core,:OFF @15:23:10.37 Gate.power :raw,:OFF @15:23:10.38 Shutdown @15:23:13.79 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_040623.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 2,\n 4 => 31,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:23:10, 17.6C, 56% humidity, 15.5psia, 13.977V, 0.257A, 0.251A avg, 3.59W",Subject: "Asleep until 15:23" @15:23:14.97
Powering core bus @15:23:15.23 upsync @15:23:17.72 upsync @15:23:20.83
Gate.power :raw,:ON @15:23:21.82 CS.configure :default @15:23:21.91 PS.configure :default @15:23:22.00 SS.configure :default @15:23:22.08 CC.configure :default @15:23:22.17 PC.configure :default @15:23:22.28 Forearm.configure :loose @15:23:22.38 Elbow.configure :loose @15:23:22.48 SC.configure :loose @15:23:22.57 SE.configure :default @15:23:22.61 CH.coast @15:23:22.66 CH.configure :default @15:23:22.69 PH.coast @15:23:22.75 PH.configure :default @15:23:22.78 All dwarf controllers running firmware version 3.85 @15:23:22.88 Valve.reset @15:23:23.09 Intake.select :close @15:23:23.14 Exhaust.select :close @15:23:23.17 CTV.dial :air @15:23:23.25 PSV.dial :air @15:23:23.33 CSV.dial :waste1 @15:23:23.41
Forearm.reconfigure :loose @15:23:23.45 Forearm.home.seek :retract @15:23:23.79 SSV.dial :spit,avoiding: :exhaust @15:23:24.19 Hand.close @15:23:24.22
Elbow.jog 1500 @15:23:24.33 PS.reconfigure :default @15:23:24.38 PS.home.seek 0ml @15:23:24.57 CS.reconfigure :fast @15:23:24.60 SS.reconfigure :default @15:23:24.70 CS.home.seek 0ml @15:23:24.73 SS.home.seek 0ml @15:23:24.84
Elbow.reconfigure :loose @15:23:24.88 Elbow.home.seek :clear @15:23:28.15 SE.reconfigure :default @15:23:28.19 SE.home.seek :down @15:23:28.78 SC.reconfigure :tight @15:23:28.82 SC.home.seek :initial @15:23:32.99 PS.stop @15:23:58.86 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @15:23:58.87
Retry #1 of 3 @15:23:58.93 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @15:24:03.33 SC.configure :limp @15:24:03.39 Forearm.seek :retract @15:24:03.44 Elbow.seek :hammer @15:24:04.56 Forearm.reconfigure :tight @15:24:04.60 Forearm.seek retracted @15:24:04.82 PC.reconfigure :default @15:24:04.84
CC.reconfigure :default @15:24:04.92 PC.home.seek :open @15:24:04.95
CC.home.seek :open @15:24:08.21 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @15:24:08.22 Notice.email "At 15:24:08 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_040623.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 2,\n 4 => 31,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:24:08, 17.6C, 56% humidity, 15.5psia, 13.880V, 0.429A, 0.429A avg, 5.95W",Subject: "Started DA_WCR_040623 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @15:24:08.24
Starting initialPurge @15:24:08.27 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:24:08.33 Hand.open @15:24:09.20 Elbow.reconfigure :tight @15:24:09.28 Elbow.seek FlushPuck::Garage @15:24:11.18 Elbow.reconfigure :loose @15:24:11.29 Forearm.reconfigure :loose @15:24:11.33 Forearm.seek :garage @15:24:12.55 Hand.close @15:24:12.66 upsync @15:24:13.44
Forearm.seek :retract @15:24:14.47 Elbow.reconfigure :tight @15:24:14.52 Elbow.seek CC @15:24:17.46 Elbow.reconfigure :loose @15:24:17.55 Forearm.reconfigure :tight @15:24:17.59 Forearm.seek CC @15:24:18.87 CC.seek :closed @15:24:23.10 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.81 @15:24:23.26 Hand.open @15:24:24.12 Forearm.reconfigure :loose @15:24:24.16 Forearm.seek :retract @15:24:25.20 Flushing the Collection Valve::Manifold @15:24:25.32 CSV.dial CSR @15:24:26.63 Pulling 5ml of CSR.flush @15:24:26.64 CSR.select :flush @15:24:26.75 CS.reconfigure :default @15:24:26.79 CS.seek 5ml @15:24:40.16 delay 5 seconds @15:24:45.18 CSR.select :air @15:24:45.32 CS.reconfigure :fast @15:24:45.36 CS.seek 7.5ml @15:24:51.84 CSV.dial :waste @15:24:53.10 CS.reconfigure :default @15:24:53.14 CS.seek 0ml @15:25:12.91 Purging external Intake @15:25:13.02 CSV.dial :flush @15:25:13.46 CS.reconfigure :fast @15:25:13.50 CS.seek 10ml @15:25:36.62 delay 5 seconds @15:25:41.65 CSV.dial CBV @15:25:41.67
CBV.dial CSV @15:25:42.42 CTV.dial :intake @15:25:43.17 Intake.select :open @15:25:43.28 CS.seek 0ml @15:26:06.06 Intake.select :close @15:26:06.16 CTV.dial :air @15:26:06.91 Flushing upper manifold @15:26:07.02 CSV.dial :flush @15:26:07.76 CS.seek 5ml @15:26:19.78 delay 5 seconds @15:26:24.80 CSV.dial :air @15:26:25.29 CS.seek 7ml @15:26:30.59 CSV.dial CBV @15:26:30.63
CBV.dial CSV @15:26:31.63 CTV.dial :air @15:26:31.64 CS.seek 0ml @15:26:47.75 delay 7 seconds @15:26:54.77 CS.seek 3ml @15:27:02.21 CSV.dial :waste @15:27:02.66 CS.seek 0ml @15:27:10.26 CSV.dial :flush @15:27:10.69 CS.seek 3ml @15:27:18.22 delay 5 seconds @15:27:23.23 CSV.dial :air @15:27:23.73 CS.seek 5ml @15:27:29.09 CSV.dial :air @15:27:29.11 CS.seek 0ml @15:27:40.87 delay 7 seconds @15:27:47.90 CS.seek 3ml @15:27:55.40 CSV.dial :waste @15:27:56.13 CS.seek 0ml @15:28:03.53 Garaging FlushPuck in Collection Clamp @15:28:03.60 Elbow.reconfigure :tight @15:28:03.64 Elbow.seek CC @15:28:03.74 Elbow.reconfigure :loose @15:28:03.83 Forearm.seek CC @15:28:04.78 Hand.close @15:28:05.56 CC.seek :open @15:28:09.85 Forearm.seek :retract @15:28:10.90 Elbow.reconfigure :tight @15:28:10.94 Elbow.seek :garage @15:28:13.92 Elbow.reconfigure :loose @15:28:14.01 Forearm.reconfigure :tight @15:28:14.04 Forearm.seek :garage @15:28:15.21 Hand.open @15:28:16.42 Forearm.reconfigure :loose @15:28:16.46 Forearm.seek :retract @15:28:17.49 da sampling at most 1000ml, wcr at most 1000ml @15:28:17.52 Loading sh1 puck from tube 4 into Collection Clamp @15:28:17.54 DAfans.email "Started da in tube 4 at 15:28:17UTC06-Apr-23\n{1 => 33,\n 2 => 33,\n 3 => 2,\n 4 => 31,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:24:08, 17.6C, 56% humidity, 15.5psia, 13.880V, 0.429A, 0.429A avg, 5.95W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @15:28:17.80
SC.reconfigure :tight @15:28:17.86 SC.seek 4 @15:28:21.51 upsync @15:28:24.55
SC.configure :limp @15:28:24.76 Elbow.reconfigure :tight @15:28:24.81 Elbow.seek 4 @15:28:26.80 Forearm.reconfigure :tight @15:28:26.85 Forearm.seek 4 + 700 counts @15:28:27.91 SE.reconfigure :pick @15:28:27.97 SE.seek :beamBreak @15:28:29.08 SE.configure :default @15:28:29.24 Elbow.seek 4 @15:28:29.61 Forearm.seek 4 + 300 counts @15:28:29.86 Forearm.reconfigure :loose @15:28:29.91 Hand.close @15:28:30.57 SE.seek :down @15:28:30.76
Forearm.seek :retract @15:28:31.31 SE.stop @15:28:31.72
Elbow.seek CC @15:28:33.34 Elbow.reconfigure :loose @15:28:33.43 Forearm.reconfigure :tight @15:28:33.48 Forearm.seek CC @15:28:34.75 CC.seek :closed @15:28:38.98 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.61 @15:28:38.99 Hand.open @15:28:39.73 Forearm.reconfigure :loose @15:28:39.78 Forearm.seek :retract @15:28:40.77 collectSample 1000ml w/bubble point of 24psi @15:28:40.78 Priming 7ml @15:28:40.79 Intake.select :close @15:28:40.80 Exhaust.select :close @15:28:40.81 SS.hold @15:28:41.00 Calibrating pressure and temperature sensors @15:28:41.03 CTV.dial :air @15:28:41.05 SSV.dial :spit,avoiding: :exhaust @15:28:41.06 SS.reconfigure :fast @15:28:41.11 SS.seek 5ml @15:28:44.04 SSV.dial CBV @15:28:44.06
CBV.dial SSV @15:28:45.40 delay 3.8 seconds @15:28:49.32 SSV.dial :spit @15:28:50.59 SS.seek 0ml @15:28:53.59 SSV.dial CBV @15:28:54.88 CTV.dial :intake @15:28:55.62 SSV.dial CBV @15:28:55.65
CBV.dial SSV @15:28:55.66 Allowing 40s for 7.0ml of remaining 7.0ml @15:28:55.67 Intake.select :open @15:28:57.82 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:28:57.84 SS.reconfigure :inhaling 2.14ml/s @15:28:57.88 SS.seek 7ml @15:29:03.58 Stroke completed -- waiting for pressure to equalize ... @15:29:03.59 SS.reconfigure :exhale @15:29:03.63 SS.hold @15:29:06.12 Intake.select :close @15:29:06.26 SSV.dial :exhaust @15:29:07.01 Exhaust.select :open @15:29:07.11 SS.seek 0ml @15:29:10.96 Exhaust.select :close @15:29:11.06 Sampling 1000.0ml @15:29:11.07 Intake.select :close @15:29:11.08 Exhaust.select :close @15:29:11.09 SS.reconfigure :default @15:29:11.13 SS.hold @15:29:11.31 CTV.dial :intake @15:29:11.32 SSV.dial CBV @15:29:11.38
CBV.dial SSV @15:29:12.08 Allowing 130s for 25.0ml of remaining 1000.0ml @15:29:12.10 Intake.select :open @15:29:14.25 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:29:14.26 SS.reconfigure :inhaling 2.14ml/s @15:29:14.30 SS.seek 25ml @15:29:20.01 Vacuum too high -- waiting up to 40 seconds @15:29:20.02 SS.reconfigure :exhale @15:29:20.06 SS.hold @15:29:20.77 Intake @11.2psia, vacuum from 5.7 to 8.7psi @15:29:20.78 SS.reconfigure :inhaling 1.93ml/s @15:29:20.82 SS.seek 25ml @15:29:22.95 Vacuum too high -- waiting up to 40 seconds @15:29:23.36 SS.reconfigure :exhale @15:29:23.48 SS.hold @15:29:24.09 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:29:24.11 SS.reconfigure :inhaling 1.74ml/s @15:29:24.15 SS.seek 25ml @15:29:26.73 Vacuum too high -- waiting up to 40 seconds @15:29:26.74 SS.reconfigure :exhale @15:29:26.78 SS.hold @15:29:27.52 Intake @12.8psia, vacuum from 7.3 to 10.3psi @15:29:27.53 SS.reconfigure :inhaling 1.57ml/s @15:29:27.57 SS.seek 25ml @15:29:29.92 Vacuum too high -- waiting up to 40 seconds @15:29:29.93 SS.reconfigure :exhale @15:29:29.97 SS.hold @15:29:30.76 Intake @13.6psia, vacuum from 8.1 to 11.1psi @15:29:30.78 SS.reconfigure :inhaling 1.41ml/s @15:29:30.82 SS.seek 25ml @15:29:34.29 Vacuum too high -- waiting up to 40 seconds @15:29:34.30 SS.reconfigure :exhale @15:29:34.35 SS.hold @15:29:35.14 Intake @13.3psia, vacuum from 7.8 to 10.8psi @15:29:35.15 SS.reconfigure :inhaling 1.27ml/s @15:29:35.20 SS.seek 25ml @15:29:41.64 Vacuum too high -- waiting up to 40 seconds @15:29:41.65 SS.reconfigure :exhale @15:29:41.69 SS.hold @15:29:42.34 Intake @12.5psia, vacuum from 7.0 to 10.0psi @15:29:42.36 SS.reconfigure :inhaling 1.14ml/s @15:29:42.40 SS.seek 25ml @15:29:43.60 Stroke completed -- waiting for pressure to equalize ... @15:29:43.61 SS.reconfigure :exhale @15:29:43.65 SS.hold @15:29:44.04 Intake.select :close @15:29:44.17 SSV.dial :exhaust @15:29:44.91 Exhaust.select :open @15:29:45.02 SS.seek 0ml @15:29:53.89 Exhaust.select :close @15:29:54.00 SSV.dial CBV @15:29:54.02
CBV.dial SSV @15:29:54.77 Allowing 130s for 25.0ml of remaining 975.0ml @15:29:54.78 Intake.select :open @15:29:56.93 Intake @15.0psia, vacuum from 9.5 to 12.5psi @15:29:56.95 SS.reconfigure :inhaling 1.14ml/s @15:29:56.99 SS.seek 25ml @15:30:06.76 Vacuum too high -- waiting up to 40 seconds @15:30:06.77 SS.reconfigure :exhale @15:30:06.81 SS.hold @15:30:07.22 Intake @12.4psia, vacuum from 6.9 to 9.9psi @15:30:07.23 SS.reconfigure :inhaling 1.03ml/s @15:30:07.27 SS.seek 25ml @15:30:24.02 Stroke completed -- waiting for pressure to equalize ... @15:30:24.03 SS.reconfigure :exhale @15:30:24.07 SS.hold @15:30:25.01 Intake.select :close @15:30:25.15 SSV.dial :exhaust @15:30:25.88 Exhaust.select :open @15:30:25.99 SS.seek 0ml @15:30:34.83 Exhaust.select :close @15:30:34.93 SSV.dial CBV @15:30:34.96
CBV.dial SSV @15:30:35.69 Allowing 130s for 25.0ml of remaining 950.0ml @15:30:35.70 Intake.select :open @15:30:37.85 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:30:37.87 SS.reconfigure :inhaling 1.03ml/s @15:30:37.91 SS.seek 25ml @15:31:04.66 Stroke completed -- waiting for pressure to equalize ... SS.reconfigure :exhale @15:31:04.71 SS.hold @15:31:05.56 Intake.select :close @15:31:05.70 SSV.dial :exhaust @15:31:06.44 Exhaust.select :open @15:31:06.55 SS.seek 0ml @15:31:15.73 Exhaust.select :close @15:31:15.84 SSV.dial CBV @15:31:15.86
CBV.dial SSV @15:31:16.60 Allowing 130s for 25.0ml of remaining 925.0ml @15:31:16.61 Intake.select :open @15:31:18.76 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:31:18.77 SS.reconfigure :inhaling 1.03ml/s @15:31:18.82 SS.seek 25ml @15:31:42.23 Vacuum too high -- waiting up to 40 seconds @15:31:42.24 SS.reconfigure :exhale @15:31:42.28 SS.hold @15:31:42.66 Intake @10.8psia, vacuum from 5.3 to 8.3psi @15:31:42.67 SS.reconfigure :inhaling 0.93ml/s @15:31:42.72 SS.seek 25ml @15:31:45.70 Stroke completed -- waiting for pressure to equalize ... @15:31:45.71 SS.reconfigure :exhale @15:31:45.75 SS.hold @15:31:46.31 Intake.select :close @15:31:46.45 SSV.dial :exhaust @15:31:47.19 Exhaust.select :open @15:31:47.29 SS.seek 0ml @15:31:56.09 Exhaust.select :close @15:31:56.19 SSV.dial CBV @15:31:56.23
CBV.dial SSV @15:31:56.94 Allowing 130s for 25.0ml of remaining 900.0ml @15:31:56.95 Intake.select :open @15:31:59.10 Intake @14.4psia, vacuum from 8.9 to 11.9psi @15:31:59.11 SS.reconfigure :inhaling 0.93ml/s @15:31:59.16 SS.seek 25ml @15:32:28.42 Stroke completed -- waiting for pressure to equalize ... @15:32:28.43 SS.reconfigure :exhale @15:32:28.47 SS.hold @15:32:29.04 Intake.select :close @15:32:29.18 SSV.dial :exhaust @15:32:29.92 Exhaust.select :open @15:32:30.03 SS.seek 0ml @15:32:38.83 Exhaust.select :close @15:32:38.93 SSV.dial CBV @15:32:38.95
CBV.dial SSV @15:32:39.69 Allowing 130s for 25.0ml of remaining 875.0ml @15:32:39.70 Intake.select :open @15:32:41.85 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:32:41.86 SS.reconfigure :inhaling 0.93ml/s @15:32:41.90 SS.seek 25ml @15:33:11.20 Stroke completed -- waiting for pressure to equalize ... @15:33:11.21 SS.reconfigure :exhale @15:33:11.25 SS.hold @15:33:12.20 Intake.select :close @15:33:12.34 SSV.dial :exhaust @15:33:13.08 Exhaust.select :open @15:33:13.18 SS.seek 0ml @15:33:22.06 Exhaust.select :close @15:33:22.16 SSV.dial CBV @15:33:22.18
CBV.dial SSV @15:33:22.92 Allowing 130s for 25.0ml of remaining 850.0ml @15:33:22.93 Intake.select :open @15:33:25.08 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:33:25.10 SS.reconfigure :inhaling 0.93ml/s @15:33:25.14 SS.seek 25ml @15:33:54.48 Stroke completed -- waiting for pressure to equalize ... @15:33:54.49 SS.reconfigure :exhale @15:33:54.53 SS.hold @15:33:55.95 Intake.select :close @15:33:56.09 SSV.dial :exhaust @15:33:56.84 Exhaust.select :open @15:33:56.95 SS.seek 0ml @15:34:05.78 Exhaust.select :close @15:34:05.88 SSV.dial CBV @15:34:05.90
CBV.dial SSV @15:34:06.64 Allowing 130s for 25.0ml of remaining 825.0ml @15:34:06.65 Intake.select :open @15:34:08.80 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:34:08.82 SS.reconfigure :inhaling 0.93ml/s @15:34:08.86 SS.seek 25ml @15:34:38.09 Stroke completed -- waiting for pressure to equalize ... @15:34:38.10 SS.reconfigure :exhale @15:34:38.14 SS.hold @15:34:39.68 Intake.select :close @15:34:39.82 SSV.dial :exhaust @15:34:40.56 Exhaust.select :open @15:34:40.66 SS.seek 0ml @15:34:49.82 Exhaust.select :close @15:34:49.93 SSV.dial CBV @15:34:49.95
CBV.dial SSV @15:34:50.68 Allowing 130s for 25.0ml of remaining 800.0ml @15:34:50.69 Intake.select :open @15:34:52.85 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:34:52.86 SS.reconfigure :inhaling 0.93ml/s @15:34:52.90 SS.seek 25ml @15:35:21.27 Stroke completed -- waiting for pressure to equalize ... @15:35:21.28 SS.reconfigure :exhale @15:35:21.32 SS.hold @15:35:22.87 Intake.select :close @15:35:23.01 SSV.dial :exhaust @15:35:23.74 Exhaust.select :open @15:35:23.85 SS.seek 0ml @15:35:32.70 Exhaust.select :close @15:35:32.80 SSV.dial CBV @15:35:32.82
CBV.dial SSV @15:35:33.56 Allowing 130s for 25.0ml of remaining 775.0ml @15:35:33.57 Intake.select :open @15:35:35.72 Intake @14.3psia, vacuum from 8.8 to 11.8psi @15:35:35.73 SS.reconfigure :inhaling 0.93ml/s @15:35:35.78 SS.seek 25ml @15:36:04.94 Stroke completed -- waiting for pressure to equalize ... @15:36:04.95 SS.reconfigure :exhale @15:36:04.99 SS.hold @15:36:10.52 Intake.select :close @15:36:10.66 SSV.dial :exhaust @15:36:11.40 Exhaust.select :open @15:36:11.51 SS.seek 0ml @15:36:20.37 Exhaust.select :close @15:36:20.47 SSV.dial CBV @15:36:20.49
CBV.dial SSV @15:36:21.23 Allowing 130s for 25.0ml of remaining 750.0ml @15:36:21.24 Intake.select :open @15:36:23.39 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:36:23.40 SS.reconfigure :inhaling 0.93ml/s @15:36:23.45 SS.seek 25ml @15:36:27.34 Vacuum too high -- waiting up to 40 seconds @15:36:27.35 SS.reconfigure :exhale @15:36:27.39 SS.hold @15:37:07.66 SS.reconfigure :default @15:37:07.70 SS.hold @15:37:07.81 Intake.select :close @15:37:07.96 ShallowSampler::Clogged in MAIN -- Intake @14.2psia, & Outlet @4.4psia -- no progress after 251.7ml @15:37:07.97 SSV.dial :exhaust @15:37:08.71 Exhaust.select :open @15:37:08.82 SS.reconfigure :exhale @15:37:08.86 SS.seek 0ml @15:37:11.18 Exhaust.select :close @15:37:11.28 Sampled 251.7ml @15:37:11.29 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:37:11.30 Intake.select :close @15:37:11.36 CSV.dial :waste @15:37:11.42 CS.seek 0ml @15:37:11.50 SSV.dial CBV,avoiding: :exhaust @15:37:11.54 CSV.dial :air @15:37:11.60
CBV.dial SSV @15:37:12.30 CS.seek 10ml @15:37:13.37
CTV.dial :air @15:37:14.11 delay 15 seconds @15:37:29.13 SSV.dial :spit,avoiding: :exhaust @15:37:30.45 CTV.dial CSV,avoiding: :intake @15:37:30.49
SS.seek 0ml @15:37:31.09 SSV.dial CBV,avoiding: :exhaust @15:37:35.82 CBV.dial between SSV and waste2 @15:37:36.20 CTV.dial CSV,avoiding: :air @15:37:36.23
CSV.dial CTV @15:37:36.68 CS.reconfigure :pressurize @15:37:36.72 CS.seek 0ml @15:37:55.73 BubblePoint: Intake @32.7psia, Outlet @8.5psia @15:37:55.74 CBV.dial SSV @15:37:56.06 SS.reconfigure :default @15:37:56.11 SS.seek 10ml @15:38:01.71 delay 15 seconds @15:38:16.83 Evacuated: Intake @16.3psia, Outlet @10.9psia @15:38:16.84 SSV.dial :spit,avoiding: :exhaust @15:38:18.17 SS.reconfigure :exhale @15:38:18.21 SS.seek 0ml @15:38:23.65 SS.reconfigure :fast @15:38:23.70 SS.seek 5ml @15:38:26.70 SSV.dial CBV,avoiding: :exhaust @15:38:26.72
CBV.dial SSV @15:38:28.02 CTV.dial :air,avoiding: :intake @15:38:29.29 CSV.dial CTV @15:38:29.33
SS.seek 10ml @15:38:30.32 CSV.dial :air @15:38:30.77 CS.reconfigure :fast @15:38:30.81 CS.seek 0ml @15:38:32.30
SSV.dial :spit,avoiding: :exhaust @15:38:33.62 SS.reconfigure :exhale @15:38:33.66 SS.seek 0ml @15:38:37.60 CS.stop @15:38:39.11
SS.reconfigure :fast @15:38:39.15 SS.seek 5ml @15:38:41.73 SSV.dial CBV,avoiding: :exhaust @15:38:41.75
CBV.dial SSV @15:38:44.06 SSV.dial :spit,avoiding: :exhaust @15:38:45.37 SS.reconfigure :default @15:38:45.41 SS.seek 0ml @15:38:48.70 SS.stop @15:38:48.73 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @15:38:48.74 Intake.select :close @15:38:48.81 CSV.dial :waste @15:38:48.92 SSV.dial CBV,avoiding: :exhaust @15:38:48.94
CBV.dial SSV @15:38:49.59 CS.seek 0ml @15:38:49.66 CSV.dial :air @15:38:50.27
CTV.dial :air @15:38:50.28 delay 15 seconds @15:38:50.39 CS.seek 10ml @15:39:05.31
SSV.dial :spit,avoiding: :exhaust @15:39:06.62 CTV.dial CSV,avoiding: :intake @15:39:06.65
SS.reconfigure :exhale @15:39:06.74 SS.seek 0ml @15:39:07.22 SSV.dial CBV,avoiding: :exhaust @15:39:13.35 CBV.dial between SSV and waste2 @15:39:13.73 CTV.dial CSV,avoiding: :air @15:39:13.75
CSV.dial CTV @15:39:14.19 CS.reconfigure :pressurize @15:39:14.23 CS.seek 0ml @15:39:35.61 BubblePoint: Intake @39.3psia, Outlet @15.1psia @15:39:35.62 CBV.dial SSV @15:39:35.94 SS.reconfigure :default @15:39:35.98 SS.seek 10ml @15:39:41.53 delay 15 seconds @15:39:56.65 Evacuated: Intake @17.9psia, Outlet @12.2psia @15:39:56.66 SSV.dial :spit,avoiding: :exhaust @15:39:57.99 SS.reconfigure :exhale @15:39:58.04 SS.seek 0ml @15:40:03.73 SS.reconfigure :fast @15:40:03.77 SS.seek 5ml @15:40:06.35 SSV.dial CBV,avoiding: :exhaust @15:40:06.37
CBV.dial SSV @15:40:07.69 CTV.dial :air,avoiding: :intake @15:40:08.99 CSV.dial CTV @15:40:09.02
SS.seek 10ml @15:40:10.02 CSV.dial :air @15:40:10.47 CS.reconfigure :fast @15:40:10.51 CS.seek 0ml @15:40:11.55
SSV.dial :spit,avoiding: :exhaust @15:40:12.88 SS.reconfigure :exhale @15:40:12.93 SS.seek 0ml @15:40:15.26 CS.stop @15:40:18.28
SS.reconfigure :fast @15:40:18.32 SS.seek 5ml @15:40:21.35 SSV.dial CBV,avoiding: :exhaust @15:40:21.37
CBV.dial SSV @15:40:23.68 SSV.dial :spit,avoiding: :exhaust @15:40:24.97 SS.reconfigure :default @15:40:25.02 SS.seek 0ml @15:40:28.12 SS.stop @15:40:28.16 Lysing: Adding 2.0ml AqMeOH @15:40:28.17 CBV.dial between CSV and waste1,avoiding: :DBV @15:40:29.63 CTV.dial CSV @15:40:30.91 CSV.dial CSR @15:40:31.80 Pulling 1ml of CSR.AqMeOH CSR.select :AqMeOH @15:40:31.91 CS.reconfigure :default @15:40:31.95 CS.seek 1ml @15:40:35.29 delay 5 seconds @15:40:40.31 CSR.select :air @15:40:40.91 CS.seek 6ml @15:40:54.38 delay 2 seconds @15:40:56.40 CSV.dial :waste2 @15:40:56.84 CS.reconfigure :fast @15:40:56.88 CS.seek 0ml @15:41:10.82 CSV.dial CSR @15:41:11.33 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @15:41:11.44 CS.reconfigure :default @15:41:11.48 CS.seek 2ml @15:41:17.38 delay 5 seconds @15:41:22.40 CSR.select :air @15:41:22.55 CS.seek 5ml @15:41:30.88 delay 2 seconds @15:41:32.90 CSV.dial CTV @15:41:33.32 CS.seek 1ml @15:41:44.55 Before lysing Collection Puck Heater at 11C/38.6psia @15:41:44.57 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @15:41:44.58 CH.seek 70C,8:00 @15:44:14.21 CH.hold 10:00,70C @15:44:14.38 Begun lysing Collection Puck Heater at 68C/43.4psia @15:54:14.39 Ended lysing Collection Puck Heater at 70C/45.4psia @15:54:14.40 CS.seek 0ml @15:54:17.77 CTV.dial between spare and air,avoiding: :intake @15:54:18.77 CSV.dial :air @15:54:19.21 CS.seek 5ml @15:54:32.61 CSV.dial CTV @15:54:33.05 delay 2 seconds @15:54:35.06 CSV.dial :air @15:54:35.52 delay 2 seconds @15:54:37.53 CSV.dial CTV @15:54:37.99 delay 2 seconds @15:54:40.01 CSV.dial :air @15:54:40.46 delay 2 seconds @15:54:42.48 CS.seek 0ml @15:54:55.94 Puck @74.0psia after final lysis pressurization @15:54:55.95 Recovering Lysate into Collection syringe @15:54:55.96 CSV.dial CBV @15:54:57.58 CBV.dial CSV @15:54:57.94 CS.seek 5.001ml @15:55:11.30 delay 5 seconds @15:55:16.31 CTV.dial :air @15:55:16.83 CS.seek 7.001ml @15:55:22.67 delay 5 seconds @15:55:27.69 CSV.dial :air2 @15:55:28.22 CS.seek 10ml @15:55:36.55 CSV.dial between air and CTV,avoiding: CBV @15:55:37.68 Lysing Complete @15:55:37.74 Storing sh1 puck in Collection Clamp to tube 3 @15:55:37.90 SC.reconfigure :tight @15:55:37.94 SC.seek 3 @15:55:41.46 SC.configure :limp @15:55:41.66 Elbow.reconfigure :tight @15:55:41.70 Elbow.seek 3 @15:55:43.45 Forearm.reconfigure :tight @15:55:43.50 Forearm.seek 3 + 700 counts @15:55:44.48 SE.reconfigure :pick @15:55:44.53 SE.seek :beamBreak @15:55:47.59 SE.configure :default @15:55:47.73 Elbow.reconfigure :limp @15:55:47.78 Forearm.reconfigure :loose @15:55:47.81 Forearm.seek 3 @15:55:48.25 Elbow.hold @15:55:48.31 Elbow.reconfigure :tight @15:55:48.40 SE.seek 1.19 Pucks below Top Plate @15:55:48.87 Forearm.force -20 @15:55:48.90 Forearm.seek 3 + 314 counts @15:55:49.17 Forearm.reconfigure :tight @15:55:49.22 Forearm.seek 3 + 700 counts @15:55:49.54 SE.seek 2.19 Pucks below Top Plate @15:55:50.02 Forearm.reconfigure :loose @15:55:50.06 Forearm.seek :retract @15:55:50.97 Elbow.seek CC @15:55:52.64 Elbow.reconfigure :loose @15:55:52.72 Forearm.seek CC @15:55:53.67 Hand.close @15:55:54.38 CC.seek :open @15:55:58.82 Forearm.seek :retract @15:55:59.81 Elbow.reconfigure :tight @15:55:59.85 Elbow.seek 3 @15:56:01.71 Elbow.reconfigure :limp @15:56:01.76 Forearm.seek 3 @15:56:02.89 Elbow.hold @15:56:02.95 Elbow.reconfigure :tight @15:56:02.99 Hand.open @15:56:03.87 Forearm.force -20 @15:56:03.90 Forearm.seek 3 + 307 counts @15:56:04.19 Forearm.reconfigure :tight @15:56:04.23 Forearm.seek 3 + 700 counts @15:56:04.60 SE.seek :down @15:56:05.72
Forearm.reconfigure :loose @15:56:05.76 Forearm.seek :retract @15:56:07.15 SE.stop @15:56:07.19
Tranferring DA @15:56:07.20 Beginning samplePassC @15:56:07.21 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:56:07.39 Elbow.seek FlushPuck::Garage @15:56:09.29 Elbow.reconfigure :loose @15:56:09.37 Forearm.seek :garage @15:56:10.47 Hand.close @15:56:11.23 Forearm.seek :retract @15:56:12.25 Elbow.reconfigure :tight @15:56:12.29 Elbow.seek CC @15:56:15.18 Elbow.reconfigure :loose @15:56:15.26 Forearm.reconfigure :tight @15:56:15.31 Forearm.seek CC @15:56:17.06 CC.seek :closed @15:56:21.28 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.86 @15:56:21.36 Hand.open @15:56:22.18 Forearm.reconfigure :loose @15:56:22.22 Forearm.seek :retract @15:56:23.31 PSV.dial PSR @15:56:24.65 Pulling 1.5ml of PSR.flush @15:56:24.66 PSR.select :flush @15:56:24.78 PS.seek 1.5ml @15:56:40.82 delay 5 seconds @15:56:45.84 PSV.dial :waste1 @15:56:46.28 PS.seek 0ml @15:57:02.41 PSV.dial PSR @15:57:02.85 PSR.select :air @15:57:02.96 PS.seek 2.5ml @15:57:29.20 PRV.dial CBV,avoiding: :ab2 @15:57:29.22
CBV.dial PRV @15:57:30.86 PSV.dial :mixloop @15:57:31.87 CTV.dial :air,avoiding: :intake @15:57:31.88 PTV.dial :PRVmixing @15:57:33.12 PS.seek 0ml @15:57:59.29 PSV.dial :air @15:57:59.73 PS.seek 2.5ml @15:58:25.97 PSV.dial :mixloop @15:58:26.43 PS.seek 0ml @15:58:52.69 PSV.dial :air @15:58:53.13 PS.seek 2.5ml @15:59:19.38 PSV.dial :mixloop @15:59:19.82 PS.seek 0ml @15:59:46.00 Garaging FlushPuck in Collection Clamp @15:59:46.07 Elbow.reconfigure :tight @15:59:46.11 Elbow.seek CC @15:59:46.22 Elbow.reconfigure :loose @15:59:46.30 Forearm.seek CC @15:59:47.26 Hand.close @15:59:47.99 CC.seek :open @15:59:52.81 Forearm.seek :retract @15:59:53.79 Elbow.reconfigure :tight @15:59:53.83 Elbow.seek :garage @15:59:56.72 Elbow.reconfigure :loose @15:59:56.81 Forearm.reconfigure :tight @15:59:56.85 Forearm.seek :garage @15:59:57.91 Hand.open @15:59:58.68 Forearm.reconfigure :loose @15:59:58.72 Forearm.seek :retract @15:59:59.77 PSV.dial :mixloop @15:59:59.78 PTV.dial :PRVmixing @15:59:59.80 PRV.dial CBV @15:59:59.82
CBV.dial PRV @15:59:59.84 CTV.dial :air,avoiding: :intake @15:59:59.85 Loading lyfil puck from tube 4 into Collection Clamp @16:00:00.07 SC.reconfigure :tight @16:00:00.11 SC.seek 4 @16:00:03.69 SC.configure :limp @16:00:03.89 Elbow.reconfigure :tight @16:00:03.93 Elbow.seek 4 @16:00:05.90 Forearm.reconfigure :tight @16:00:05.94 Forearm.seek 4 + 700 counts @16:00:06.88 SE.reconfigure :pick @16:00:06.93 SE.seek :beamBreak @16:00:08.07 SE.configure :default @16:00:08.21 Elbow.seek 4 @16:00:08.57 Forearm.seek 4 + 300 counts @16:00:08.75 Forearm.reconfigure :loose @16:00:08.79 Hand.close @16:00:09.38 SE.seek :down @16:00:09.57
Forearm.seek :retract @16:00:10.65 SE.stop @16:00:10.72
Elbow.seek CC @16:00:12.27 Elbow.reconfigure :loose @16:00:12.36 Forearm.reconfigure :tight @16:00:12.40 Forearm.seek CC @16:00:13.66 CC.seek :closed @16:00:17.89 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.63 @16:00:17.90 Hand.open @16:00:18.68 Forearm.reconfigure :loose @16:00:18.72 Forearm.seek :retract @16:00:19.68 CTV.dial CSV,avoiding: :intake @16:00:20.98 CSV.dial CTV @16:00:21.28 PS.seek 2.5ml @16:00:47.82 CS.seek 5.5ml @16:00:59.98 delay 8 seconds @16:01:07.99 PSV.dial :air @16:01:08.44 delay 2 seconds @16:01:10.45 PSV.dial :waste2 @16:01:11.18 PS.seek 0ml @16:01:37.66 CBV.dial between PRV and waste1 @16:01:37.98 CSV.dial :air @16:01:38.43 CS.seek 10ml @16:01:50.57 CSV.dial CTV @16:01:51.12 CS.seek 0ml @16:02:17.13 CBV.dial :waste1 @16:02:17.43 delay 5 seconds @16:02:22.45 CTV.dial :air,avoiding: :intake @16:02:23.78 Storing lyfil puck in Collection Clamp to tube 3 @16:02:23.94 SC.reconfigure :tight @16:02:23.98 SC.seek 3 @16:02:27.55 SC.configure :limp @16:02:27.74 Elbow.reconfigure :tight @16:02:27.78 Elbow.seek 3 @16:02:29.60 Forearm.reconfigure :tight @16:02:29.64 Forearm.seek 3 + 700 counts @16:02:30.72 SE.reconfigure :pick @16:02:30.77 SE.seek :beamBreak @16:02:33.78 SE.configure :default @16:02:33.93 Elbow.reconfigure :limp @16:02:33.97 Forearm.reconfigure :loose @16:02:34.01 Forearm.seek 3 @16:02:34.41 Elbow.hold @16:02:34.47 Elbow.reconfigure :tight @16:02:34.56 SE.seek 2.2 Pucks below Top Plate @16:02:34.98 Forearm.force -20 @16:02:35.02 Forearm.seek 3 + 304 counts @16:02:35.32 Forearm.reconfigure :tight @16:02:35.36 Forearm.seek 3 + 700 counts @16:02:35.57 SE.seek 3.2 Pucks below Top Plate @16:02:36.04 Forearm.reconfigure :loose @16:02:36.08 Forearm.seek :retract @16:02:37.02 Elbow.seek CC @16:02:38.62 Elbow.reconfigure :loose @16:02:38.71 Forearm.seek CC @16:02:39.64 Hand.close @16:02:40.24 CC.seek :open @16:02:44.68 Forearm.seek :retract @16:02:45.68 Elbow.reconfigure :tight @16:02:45.72 Elbow.seek 3 @16:02:47.58 Elbow.reconfigure :limp @16:02:47.62 Forearm.seek 3 @16:02:48.75 Elbow.hold @16:02:49.28 Elbow.reconfigure :tight @16:02:49.32 Hand.open @16:02:50.22 Forearm.force -20 @16:02:50.26 Forearm.seek 3 + 316 counts @16:02:50.62 Forearm.reconfigure :tight @16:02:50.66 Forearm.seek 3 + 700 counts @16:02:50.96 SE.seek :down @16:02:52.08
Forearm.reconfigure :loose @16:02:52.12 Forearm.seek :retract @16:02:53.44 SE.stop @16:02:53.47
PSV.dial :air @16:02:54.21 PS.seek 2.5ml @16:03:20.47 delay 1 second @16:03:21.48 PSV.dial :mixloop @16:03:21.94 delay 1 second @16:03:22.95 PSV.dial :air @16:03:23.39 delay 1 second @16:03:24.40 PSV.dial :mixloop @16:03:24.84 delay 1 second @16:03:25.85 PSV.dial :air @16:03:26.31 delay 1 second @16:03:27.33 PS.seek 0ml @16:03:53.50 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:03:53.67 Elbow.seek FlushPuck::Garage @16:03:55.51 Elbow.reconfigure :loose @16:03:55.59 Forearm.seek :garage @16:03:56.70 Hand.close @16:03:57.47 Forearm.seek :retract @16:03:58.53 Elbow.reconfigure :tight @16:03:58.58 Elbow.seek PC @16:03:59.90 Elbow.reconfigure :loose @16:03:59.99 Forearm.reconfigure :tight @16:04:00.03 Forearm.seek PC @16:04:01.40 PC.seek :closed @16:04:05.28 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.39 @16:04:05.34 Hand.open @16:04:06.17 Forearm.reconfigure :loose @16:04:06.21 Forearm.seek :retract @16:04:07.23 PRV.dial :air @16:04:07.68 PSV.dial :mixloop @16:04:08.20 PS.seek 0.3ml @16:04:12.06 PSV.dial PSR @16:04:13.09 Pulling 0.4ml of PSR.air @16:04:13.10 PSR.select :air @16:04:13.13 PS.seek 0.7ml @16:04:18.06 delay 3 seconds @16:04:21.07 PSR.select :air @16:04:21.09 PS.seek 2.5ml @16:04:40.15 delay 3 seconds @16:04:43.17 PSV.dial :mixloop @16:04:44.15 PTV.dial :mixing @16:04:44.87 PBV.dial :waste2 @16:04:45.76 PS.seek 0ml @16:05:11.93 Garaging FlushPuck in Processing Clamp @16:05:12.00 Elbow.reconfigure :tight @16:05:12.05 Elbow.seek PC @16:05:12.16 Elbow.reconfigure :loose @16:05:12.25 Forearm.seek PC @16:05:13.34 Hand.close @16:05:13.99 PC.seek :open @16:05:17.95 Forearm.seek :retract @16:05:19.02 Elbow.reconfigure :tight @16:05:19.06 Elbow.seek :garage @16:05:20.43 Elbow.reconfigure :loose @16:05:20.51 Forearm.reconfigure :tight @16:05:20.56 Forearm.seek :garage @16:05:21.72 Hand.open @16:05:22.54 Forearm.reconfigure :loose @16:05:22.58 Forearm.seek :retract @16:05:23.62 Loading da puck from tube 4 into Processing Clamp @16:05:24.31 SC.reconfigure :tight @16:05:24.35 SC.seek 4 @16:05:27.89 SC.configure :limp @16:05:28.08 Elbow.reconfigure :tight @16:05:28.12 Elbow.seek 4 @16:05:30.05 Forearm.reconfigure :tight @16:05:30.09 Forearm.seek 4 + 700 counts @16:05:31.06 SE.reconfigure :pick @16:05:31.11 SE.seek :beamBreak @16:05:32.39 SE.configure :default @16:05:32.54 Elbow.seek 4 @16:05:32.71 Forearm.seek 4 + 300 counts @16:05:32.98 Forearm.reconfigure :loose @16:05:33.03 Hand.close @16:05:33.62 SE.seek :down @16:05:33.81
Forearm.seek :retract @16:05:34.48 SE.stop @16:05:34.77
Elbow.seek PC @16:05:37.30 Elbow.reconfigure :loose @16:05:37.39 Forearm.reconfigure :tight @16:05:37.43 Forearm.seek PC @16:05:38.81 PC.seek :closed @16:05:42.72 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.35 @16:05:42.73 Hand.open @16:05:43.54 Forearm.reconfigure :loose @16:05:43.58 Forearm.seek :retract @16:05:44.63 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @16:05:44.65 PH.seek 28C,5:00 @16:06:36.01 PH.hold 2:00:00,28C @16:06:36.05 delay 1:30 @16:08:06.07 PSR.select :air @16:08:06.09 PSV.dial PSR @16:08:07.15 Pulling 0.5ml of PSR.wash @16:08:07.16 PSR.select :wash @16:08:07.27 PS.seek 0.5ml @16:08:13.15 delay 5 seconds @16:08:18.17 PSR.select :air @16:08:18.28 PS.seek 2ml @16:08:34.27 PTV.dial :puck @16:08:34.97 PSV.dial PTV @16:08:35.69 PBV.dial :waste1 @16:08:37.00 PS.seek 1.5ml @16:08:42.86 delay 3 seconds @16:08:45.88 PBV.dial between PSV and waste1 @16:08:46.25 PS.seek 0ml @16:09:02.33 delay 5:00 @16:14:02.35 recoverPSRreagent08 @16:14:02.36 PTV.dial :puck @16:14:02.37 PBV.dial :waste1 @16:14:02.71 delay 30 seconds @16:14:32.74 PBV.dial between PSV and waste1 @16:14:33.09 PSR.select :air @16:14:33.11 PSV.dial PSR @16:14:33.84 PS.reconfigure :fast @16:14:33.89 PS.seek 2.5ml @16:14:56.73 PSV.dial PTV @16:14:57.45 delay 5 seconds @16:15:02.47 PSV.dial PSR @16:15:03.20 delay 5 seconds @16:15:08.22 PSV.dial PTV @16:15:08.95 delay 5 seconds @16:15:14.36 PSV.dial :air @16:15:15.16 PS.seek 0ml @16:15:37.79 PTV.dial :PRVmixing,via: PRV @16:15:39.06 PSV.dial :mixloop @16:15:39.49 PRV.dial :ab1 @16:15:40.25 PS.reconfigure :slow @16:15:40.29 PS.seek 0.15ml @16:15:43.85 delay 2 seconds @16:15:45.87 CBV.dial PRV @16:15:45.89
PRV.dial CBV @16:15:46.47 PS.seek 0.34ml @16:15:50.82 delay 2 seconds @16:15:52.84 CBV.dial between PRV and PSV @16:15:53.14 PRV.dial :air @16:15:53.59 PS.seek 0.9ml @16:16:05.51 PRV.dial :waste2 @16:16:06.22 PS.seek 0.5ml @16:16:14.82 addPMLReagent08 @16:16:14.83 PSV.dial :air @16:16:15.28 PS.reconfigure :default @16:16:15.32 PS.seek 2ml @16:16:31.38 PSV.dial :PML @16:16:31.82 PTV.dial :mixing @16:16:32.55 PBV.dial :waste1 @16:16:32.97 PS.seek 1.85ml @16:16:35.29 PBV.dial between PSV and waste1 @16:16:35.67 PS.seek 0ml @16:16:55.29 delay 2 seconds @16:16:57.31 Beginning samplePassC @16:16:57.32 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:16:57.48 Elbow.reconfigure :tight @16:16:57.52 Elbow.seek FlushPuck::Garage @16:16:59.12 Elbow.reconfigure :loose @16:16:59.21 Forearm.seek :garage @16:17:00.34 Hand.close @16:17:01.20 Forearm.seek :retract @16:17:02.23 Elbow.reconfigure :tight @16:17:02.27 Elbow.seek CC @16:17:05.14 Elbow.reconfigure :loose @16:17:05.23 Forearm.reconfigure :tight @16:17:05.27 Forearm.seek CC @16:17:06.53 CC.seek :closed @16:17:10.75 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.76 @16:17:10.83 Hand.open @16:17:11.64 Forearm.reconfigure :loose @16:17:11.68 Forearm.seek :retract @16:17:12.65 PSV.dial PSR @16:17:13.69 Pulling 1.5ml of PSR.flush PSR.select :flush @16:17:13.81 PS.seek 1.5ml @16:17:30.07 delay 5 seconds @16:17:35.09 PSV.dial :waste1 @16:17:35.54 PS.seek 0ml @16:17:51.63 PSV.dial PSR @16:17:52.07 PSR.select :air @16:17:52.18 PS.seek 2.5ml @16:18:18.32 PRV.dial CBV,avoiding: :ab2 @16:18:18.34
CBV.dial PRV @16:18:19.96 PSV.dial :mixloop @16:18:20.98 CTV.dial :air,avoiding: :intake @16:18:20.99 PTV.dial :PRVmixing @16:18:21.71 PS.seek 0ml @16:18:47.88 PSV.dial :air @16:18:48.32 PS.seek 2.5ml @16:19:14.44 PSV.dial :mixloop @16:19:14.90 PS.seek 0ml @16:19:41.03 PSV.dial :air @16:19:41.48 PS.seek 2.5ml @16:20:07.63 PSV.dial :mixloop @16:20:08.07 PS.seek 0ml @16:20:34.27 Garaging FlushPuck in Collection Clamp @16:20:34.34 Elbow.reconfigure :tight @16:20:34.38 Elbow.seek CC @16:20:34.49 Elbow.reconfigure :loose @16:20:34.59 Forearm.seek CC @16:20:35.55 Hand.close @16:20:36.33 CC.seek :open @16:20:40.68 Forearm.seek :retract @16:20:41.67 Elbow.reconfigure :tight @16:20:41.72 Elbow.seek :garage @16:20:44.65 Elbow.reconfigure :loose @16:20:44.74 Forearm.reconfigure :tight @16:20:44.78 Forearm.seek :garage @16:20:45.91 Hand.open @16:20:46.76 Forearm.reconfigure :loose @16:20:46.81 Forearm.seek :retract @16:20:47.83 delayUntil "16:24:57.29" @16:20:47.86 Whole Cell Archive sampling 1000ml @16:20:47.87 Loading wcr puck from tube 4 into Collection Clamp @16:20:48.09 SC.reconfigure :tight @16:20:48.14 SC.seek 4 @16:20:48.20 SC.configure :limp @16:20:48.40 Elbow.reconfigure :tight @16:20:48.44 Elbow.seek 4 @16:20:50.43 Forearm.reconfigure :tight @16:20:50.48 Forearm.seek 4 + 700 counts @16:20:51.44 SE.reconfigure :pick @16:20:51.49 SE.seek :beamBreak @16:20:52.86 SE.configure :default @16:20:53.01 Elbow.seek 4 @16:20:53.18 Forearm.seek 4 + 300 counts @16:20:53.42 Forearm.reconfigure :loose @16:20:53.47 Hand.close @16:20:54.09 SE.seek :down @16:20:54.28 Forearm.seek :retract @16:20:55.04 SE.stop @16:20:55.25 Elbow.seek CC @16:20:56.77 Elbow.reconfigure :loose @16:20:56.86 Forearm.reconfigure :tight @16:20:56.90 Forearm.seek CC @16:20:58.21 CC.seek :closed @16:21:02.45 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.76 @16:21:02.46 Hand.open @16:21:03.26 Forearm.reconfigure :loose @16:21:03.31 Forearm.seek :retract @16:21:04.25 collectSample 1000ml w/bubble point of 35psi @16:21:04.27 Priming 7ml @16:21:04.28 Intake.select :close @16:21:04.29 Exhaust.select :close @16:21:04.30 SS.hold @16:21:04.49 Calibrating pressure sensors @16:21:04.50 CTV.dial :air @16:21:04.51 SSV.dial :spit,avoiding: :exhaust @16:21:04.53 SS.reconfigure :fast @16:21:04.58 SS.seek 5ml @16:21:07.37 SSV.dial CBV @16:21:07.39 CBV.dial SSV @16:21:08.72 delay 3.8 seconds @16:21:12.65 SSV.dial :spit @16:21:13.96 SS.seek 0ml @16:21:17.08 SSV.dial CBV @16:21:18.86 CTV.dial :intake @16:21:19.59 SSV.dial CBV @16:21:19.61 CBV.dial SSV @16:21:19.64 Allowing 40s for 7.0ml of remaining 7.0ml @16:21:19.65 Intake.select :open @16:21:21.80 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:21:21.82 SS.reconfigure :inhaling 2.14ml/s @16:21:21.86 SS.seek 7ml @16:21:27.45 Stroke completed -- waiting for pressure to equalize ... @16:21:27.47 SS.reconfigure :exhale @16:21:27.51 SS.hold @16:21:27.80 Intake.select :close @16:21:27.95 SSV.dial :exhaust @16:21:28.69 Exhaust.select :open @16:21:28.81 SS.seek 0ml @16:21:32.88 Exhaust.select :close @16:21:32.99 Sampling 1000.0ml @16:21:33.00 Intake.select :close @16:21:33.01 Exhaust.select :close @16:21:33.02 SS.reconfigure :default @16:21:33.06 SS.hold @16:21:33.25 CTV.dial :intake @16:21:33.27 SSV.dial CBV @16:21:33.29 CBV.dial SSV @16:21:34.05 Allowing 130s for 25.0ml of remaining 1000.0ml @16:21:34.06 Intake.select :open @16:21:36.22 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:21:36.24 SS.reconfigure :inhaling 2.14ml/s @16:21:36.28 SS.seek 25ml @16:21:47.88 Vacuum too high -- waiting up to 40 seconds @16:21:47.90 SS.reconfigure :exhale @16:21:47.94 SS.hold @16:22:04.53 SS.reconfigure :inhaling 1.93ml/s @16:22:04.57 SS.seek 25ml @16:22:09.36 Stroke completed -- waiting for pressure to equalize ... @16:22:09.37 SS.reconfigure :exhale @16:22:09.42 SS.hold @16:22:27.68 Intake.select :close @16:22:27.83 SSV.dial :exhaust @16:22:28.58 Exhaust.select :open @16:22:28.69 SS.seek 0ml @16:22:37.78 Exhaust.select :close @16:22:37.89 SSV.dial CBV @16:22:37.91 CBV.dial SSV @16:22:38.67 Allowing 130s for 25.0ml of remaining 975.0ml @16:22:38.69 Intake.select :open @16:22:40.84 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:22:40.86 SS.reconfigure :inhaling 1.93ml/s @16:22:40.90 SS.seek 25ml @16:22:44.69 Vacuum too high -- waiting up to 40 seconds @16:22:44.71 SS.reconfigure :exhale @16:22:44.75 SS.hold @16:22:46.53 Intake @16.6psia, vacuum from 10.0 to 13.0psi @16:22:46.54 SS.reconfigure :inhaling 1.74ml/s @16:22:46.59 SS.seek 25ml @16:22:48.29 Vacuum too high -- waiting up to 40 seconds @16:22:48.30 SS.reconfigure :exhale @16:22:48.83 SS.hold @16:22:49.34 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:22:49.36 SS.reconfigure :inhaling 1.57ml/s @16:22:49.40 SS.seek 25ml @16:22:51.48 Vacuum too high -- waiting up to 40 seconds @16:22:51.49 SS.reconfigure :exhale @16:22:51.53 SS.hold @16:22:53.46 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:22:53.48 SS.reconfigure :inhaling 1.41ml/s @16:22:53.52 SS.seek 25ml @16:22:55.23 Vacuum too high -- waiting up to 40 seconds @16:22:55.24 SS.reconfigure :exhale @16:22:55.28 SS.hold @16:22:56.66 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:22:56.67 SS.reconfigure :inhaling 1.27ml/s @16:22:56.72 SS.seek 25ml @16:22:58.32 Vacuum too high -- waiting up to 40 seconds @16:22:58.33 SS.reconfigure :exhale @16:22:58.38 SS.hold @16:22:59.38 Intake @15.6psia, vacuum from 10.0 to 13.0psi @16:22:59.40 SS.reconfigure :inhaling 1.14ml/s @16:22:59.44 SS.seek 25ml @16:23:01.76 Vacuum too high -- waiting up to 40 seconds @16:23:01.78 SS.reconfigure :exhale @16:23:01.82 SS.hold @16:23:03.11 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:23:03.13 SS.reconfigure :inhaling 1.03ml/s @16:23:03.17 SS.seek 25ml @16:23:05.63 Vacuum too high -- waiting up to 40 seconds @16:23:05.64 SS.reconfigure :exhale @16:23:05.69 SS.hold @16:23:06.99 Intake @15.5psia, vacuum from 10.0 to 13.0psi @16:23:07.00 SS.reconfigure :inhaling 0.93ml/s @16:23:07.05 SS.seek 25ml @16:23:09.85 Vacuum too high -- waiting up to 40 seconds @16:23:09.86 SS.reconfigure :exhale @16:23:09.91 SS.hold @16:23:11.58 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:23:11.60 SS.reconfigure :inhaling 0.84ml/s @16:23:11.64 SS.seek 25ml @16:23:13.95 Vacuum too high -- waiting up to 40 seconds @16:23:13.96 SS.reconfigure :exhale @16:23:14.00 SS.hold @16:23:14.96 Intake @16.3psia, vacuum from 10.0 to 13.0psi @16:23:14.98 SS.reconfigure :inhaling 0.76ml/s @16:23:15.02 SS.seek 25ml @16:23:18.51 Vacuum too high -- waiting up to 40 seconds @16:23:18.52 SS.reconfigure :exhale @16:23:18.56 SS.hold @16:23:20.20 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:23:20.22 SS.reconfigure :inhaling 0.69ml/s @16:23:20.27 SS.seek 25ml @16:23:25.48 Vacuum too high -- waiting up to 40 seconds @16:23:25.49 SS.reconfigure :exhale @16:23:25.53 SS.hold @16:23:27.18 Intake @16.2psia, vacuum from 10.0 to 13.0psi @16:23:27.20 SS.reconfigure :inhaling 0.61ml/s @16:23:27.24 SS.seek 25ml @16:23:32.47 Stroke completed -- waiting for pressure to equalize ... @16:23:32.48 SS.reconfigure :exhale @16:23:32.52 SS.hold @16:23:35.26 Intake.select :close @16:23:35.41 SSV.dial :exhaust @16:23:36.16 Exhaust.select :open @16:23:36.27 SS.seek 0ml @16:23:45.87 Exhaust.select :close @16:23:45.98 SSV.dial CBV @16:23:46.00 CBV.dial SSV @16:23:46.77 Allowing 130s for 25.0ml of remaining 950.0ml @16:23:46.78 Intake.select :open @16:23:48.94 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:23:48.96 SS.reconfigure :inhaling 0.61ml/s @16:23:49.00 SS.seek 25ml @16:24:05.29 Vacuum too high -- waiting up to 40 seconds @16:24:05.30 SS.reconfigure :exhale @16:24:05.34 SS.hold @16:24:07.00 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:24:07.02 SS.reconfigure :inhaling 0.56ml/s @16:24:07.06 SS.seek 25ml @16:24:17.97 Vacuum too high -- waiting up to 40 seconds @16:24:17.99 SS.reconfigure :exhale @16:24:18.03 SS.hold @16:24:19.31 Intake @15.7psia, vacuum from 10.0 to 13.0psi @16:24:19.33 SS.reconfigure :inhaling 0.50ml/s @16:24:19.37 SS.seek 25ml @16:24:39.76 Stroke completed -- waiting for pressure to equalize ... @16:24:39.77 SS.reconfigure :exhale @16:24:39.82 SS.hold @16:24:43.88 Intake.select :close @16:24:44.03 SSV.dial :exhaust @16:24:44.79 Exhaust.select :open @16:24:44.90 SS.seek 0ml @16:24:54.29 Exhaust.select :close @16:24:54.40 SSV.dial CBV @16:24:54.42 CBV.dial SSV @16:24:55.19 Allowing 130s for 25.0ml of remaining 925.0ml @16:24:55.20 Intake.select :open @16:24:57.30 recoverPRVreagent08 @16:24:57.31 PTV.dial :mixing @16:24:57.40 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:24:57.42 SS.reconfigure :inhaling 0.50ml/s @16:24:57.46 SS.seek 25ml @16:24:58.04 PBV.dial :waste1 @16:24:58.42 delay 30 seconds @16:25:28.44 PBV.dial between PSV and waste1 @16:25:28.79 PSV.dial :air @16:25:29.25 PS.reconfigure :fast @16:25:29.29 PS.seek 2.5ml @16:25:49.04 Stroke completed -- waiting for pressure to equalize ... @16:25:49.05 SS.reconfigure :exhale @16:25:49.09 SS.hold @16:25:52.33 PSV.dial :mixloop @16:25:52.77 delay 2 seconds @16:25:53.86 Intake.select :close @16:25:54.01 SSV.dial :exhaust @16:25:54.75 Exhaust.select :open @16:25:54.80 PSV.dial :air @16:25:54.86 SS.seek 0ml @16:25:55.24 delay 2 seconds @16:25:57.26 PSV.dial :mixloop @16:25:58.15 delay 2 seconds @16:26:00.17 PSV.dial :air @16:26:00.62 PS.seek 0ml @16:26:03.92 Exhaust.select :close @16:26:04.02 SSV.dial CBV @16:26:04.08 CBV.dial SSV @16:26:04.78 Allowing 130s for 25.0ml of remaining 900.0ml @16:26:04.79 Intake.select :open @16:26:06.95 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:26:06.97 SS.reconfigure :inhaling 0.50ml/s @16:26:07.01 SS.seek 25ml @16:26:23.59 washCycleDA #1 of 2 @16:26:23.60 PSR.select :air @16:26:23.61 PSV.dial PSR @16:26:24.95 Pulling 0.5ml of PSR.wash @16:26:24.96 PSR.select :wash @16:26:25.08 PS.reconfigure :default @16:26:25.12 PS.seek 0.5ml @16:26:31.03 delay 5 seconds @16:26:36.05 PSR.select :air @16:26:36.16 PS.seek 2ml @16:26:46.66 Vacuum too high -- waiting up to 40 seconds @16:26:46.67 SS.reconfigure :exhale @16:26:46.71 SS.hold @16:26:49.22 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:26:49.23 SS.reconfigure :inhaling 0.46ml/s @16:26:49.28 SS.seek 25ml @16:26:52.18 PTV.dial :puck @16:26:52.90 PSV.dial PTV @16:26:53.63 PBV.dial :waste1 @16:26:54.05 PS.seek 1.5ml @16:26:59.96 delay 3 seconds @16:27:02.98 PBV.dial between PSV and waste1 @16:27:03.34 PS.seek 0ml @16:27:04.13 Stroke completed -- waiting for pressure to equalize ... @16:27:04.14 SS.reconfigure :exhale @16:27:04.19 SS.hold @16:27:08.96 Intake.select :close @16:27:09.10 SSV.dial :exhaust @16:27:09.85 Exhaust.select :open @16:27:09.97 SS.seek 0ml @16:27:19.02 Exhaust.select :close @16:27:19.13 SSV.dial CBV @16:27:19.15 CBV.dial SSV @16:27:19.40 delay 2:00 @16:27:19.92 Allowing 130s for 25.0ml of remaining 875.0ml @16:27:19.93 Intake.select :open @16:27:22.09 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:27:22.11 SS.reconfigure :inhaling 0.46ml/s @16:27:22.15 SS.seek 25ml @16:28:19.82 Stroke completed -- waiting for pressure to equalize ... @16:28:19.83 SS.reconfigure :exhale @16:28:19.87 SS.hold @16:28:26.27 Intake.select :close @16:28:26.41 SSV.dial :exhaust @16:28:27.16 Exhaust.select :open @16:28:27.27 SS.seek 0ml @16:28:36.72 Exhaust.select :close @16:28:36.83 SSV.dial CBV @16:28:36.85 CBV.dial SSV @16:28:37.60 Allowing 130s for 25.0ml of remaining 850.0ml @16:28:37.61 Intake.select :open @16:28:39.77 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:28:39.79 SS.reconfigure :inhaling 0.46ml/s @16:28:39.83 SS.seek 25ml @16:29:09.78 Vacuum too high -- waiting up to 40 seconds @16:29:09.79 SS.reconfigure :exhale @16:29:09.83 SS.hold @16:29:12.72 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:29:12.74 SS.reconfigure :inhaling 0.41ml/s @16:29:12.78 SS.seek 25ml @16:29:19.42 recoverPSRreagent08 @16:29:19.43 PTV.dial :puck @16:29:19.44 PBV.dial :waste1 @16:29:19.80 delay 30 seconds @16:29:42.56 Stroke completed -- waiting for pressure to equalize ... @16:29:42.58 SS.reconfigure :exhale @16:29:43.02 SS.hold @16:29:48.60 Intake.select :close @16:29:48.74 SSV.dial :exhaust @16:29:49.49 Exhaust.select :open @16:29:49.60 SS.seek 0ml @16:29:49.82 PBV.dial between PSV and waste1 @16:29:50.18 PSR.select :air @16:29:50.20 PSV.dial PSR @16:29:50.93 PS.reconfigure :fast @16:29:50.98 PS.seek 2.5ml @16:29:59.06 Exhaust.select :close @16:29:59.17 SSV.dial CBV @16:29:59.21 CBV.dial SSV @16:29:59.96 Allowing 130s for 25.0ml of remaining 825.0ml @16:29:59.97 Intake.select :open @16:30:02.13 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:30:02.15 SS.reconfigure :inhaling 0.41ml/s @16:30:02.19 SS.seek 25ml @16:30:14.30 PSV.dial PTV @16:30:15.03 delay 5 seconds @16:30:20.05 PSV.dial PSR @16:30:20.91 delay 5 seconds @16:30:25.93 PSV.dial PTV @16:30:26.64 delay 5 seconds @16:30:31.66 PSV.dial :air @16:30:32.40 PS.seek 0ml @16:30:55.30 washCycleDA #2 of 2 @16:30:55.31 PSR.select :air @16:30:55.33 PSV.dial PSR @16:30:56.65 Pulling 0.5ml of PSR.wash @16:30:56.66 PSR.select :wash @16:30:56.77 PS.reconfigure :default @16:30:56.81 PS.seek 0.5ml @16:31:00.44 Vacuum too high -- waiting up to 40 seconds @16:31:00.45 SS.reconfigure :exhale @16:31:00.49 SS.hold @16:31:02.74 delay 5 seconds @16:31:05.29 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:31:05.30 SS.reconfigure :inhaling 0.37ml/s @16:31:05.35 SS.seek 25ml @16:31:07.75 PSR.select :air @16:31:07.86 PS.seek 2ml @16:31:11.41 Stroke completed -- waiting for pressure to equalize ... @16:31:11.42 SS.reconfigure :exhale @16:31:11.46 SS.hold @16:31:16.33 Intake.select :close @16:31:16.47 SSV.dial :exhaust @16:31:17.22 Exhaust.select :open @16:31:17.33 SS.seek 0ml @16:31:23.90 PTV.dial :puck @16:31:23.91 PSV.dial PTV @16:31:24.64 PBV.dial :waste1 @16:31:25.06 PS.seek 1.5ml @16:31:26.37 Exhaust.select :close @16:31:26.48 SSV.dial CBV @16:31:26.51 CBV.dial SSV @16:31:27.25 Allowing 130s for 25.0ml of remaining 800.0ml @16:31:27.26 Intake.select :open @16:31:29.42 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:31:29.44 SS.reconfigure :inhaling 0.37ml/s @16:31:29.48 SS.seek 25ml @16:31:30.98 delay 3 seconds @16:31:34.00 PBV.dial between PSV and waste1 @16:31:34.38 PS.seek 0ml @16:31:50.39 delay 2:00 @16:32:42.81 Stroke completed -- waiting for pressure to equalize ... @16:32:42.82 SS.reconfigure :exhale @16:32:42.86 SS.hold @16:32:51.90 Intake.select :close @16:32:52.05 SSV.dial :exhaust @16:32:52.80 Exhaust.select :open @16:32:52.91 SS.seek 0ml @16:33:01.96 Exhaust.select :close @16:33:02.07 SSV.dial CBV @16:33:02.10 CBV.dial SSV @16:33:02.83 Allowing 130s for 25.0ml of remaining 775.0ml @16:33:02.84 Intake.select :open @16:33:05.00 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:33:05.02 SS.reconfigure :inhaling 0.37ml/s @16:33:05.06 SS.seek 25ml @16:33:50.36 Vacuum too high -- waiting up to 40 seconds @16:33:50.37 SS.reconfigure :exhale @16:33:50.42 SS.hold @16:33:50.49 recoverPSRreagent08 @16:33:50.50 PTV.dial :puck @16:33:50.51 PBV.dial :waste1 @16:33:50.89 delay 30 seconds @16:33:55.96 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:33:55.98 SS.reconfigure :inhaling 0.33ml/s @16:33:56.02 SS.seek 25ml @16:34:20.91 PBV.dial between PSV and waste1 @16:34:21.28 PSR.select :air @16:34:21.30 PSV.dial PSR @16:34:22.03 PS.reconfigure :fast @16:34:22.08 PS.seek 2.5ml @16:34:28.48 Stroke completed -- waiting for pressure to equalize ... @16:34:28.49 SS.reconfigure :exhale @16:34:28.53 SS.hold @16:34:38.21 Intake.select :close @16:34:38.35 SSV.dial :exhaust @16:34:39.09 Exhaust.select :open @16:34:39.20 SS.seek 0ml @16:34:45.23 PSV.dial PTV @16:34:45.96 delay 5 seconds @16:34:48.63 Exhaust.select :close @16:34:48.74 SSV.dial CBV @16:34:48.76 CBV.dial SSV @16:34:49.50 Allowing 130s for 25.0ml of remaining 750.0ml @16:34:49.51 Intake.select :open @16:34:50.98 PSV.dial PSR @16:34:51.66 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:34:51.68 SS.reconfigure :inhaling 0.33ml/s @16:34:51.74 SS.seek 25ml @16:34:51.76 delay 5 seconds @16:34:56.79 PSV.dial PTV @16:34:57.53 delay 5 seconds @16:35:02.55 PSV.dial :air @16:35:03.28 PS.seek 0ml @16:35:26.32 getPMLReagent08 @16:35:26.33 PRV.dial :air @16:35:26.78 delay 5 seconds @16:35:31.80 PTV.dial :PRVmixing @16:35:33.09 delay 5 seconds @16:35:38.11 PRV.dial :ab2 @16:35:38.54 PTV.dial :PRVmixing @16:35:38.55 PSV.dial :mixloop @16:35:39.00 PS.reconfigure :default @16:35:39.04 PS.seek 0.3ml @16:35:42.88 delay 2 seconds @16:35:44.90 PRV.dial :air @16:35:45.40 PS.seek 0.55ml @16:35:48.82 addPMLReagent08 @16:35:48.88 PSV.dial :air @16:35:49.82 PS.seek 2ml @16:36:05.38 PSV.dial :PML @16:36:05.82 PTV.dial :mixing @16:36:06.56 PBV.dial :waste1 @16:36:06.99 PS.seek 1.85ml @16:36:09.35 PBV.dial between PSV and waste1 @16:36:09.74 PS.seek 0ml @16:36:15.83 Stroke completed -- waiting for pressure to equalize ... @16:36:15.85 SS.reconfigure :exhale @16:36:15.89 SS.hold @16:36:29.32 delay 2 seconds @16:36:31.34 delay 8:00 @16:36:32.07 Intake.select :close @16:36:32.22 SSV.dial :exhaust @16:36:32.94 Exhaust.select :open @16:36:33.05 SS.seek 0ml @16:36:42.55 Exhaust.select :close @16:36:42.66 SSV.dial CBV @16:36:42.68 CBV.dial SSV @16:36:43.44 Allowing 130s for 25.0ml of remaining 725.0ml @16:36:43.46 Intake.select :open @16:36:45.61 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:36:45.63 SS.reconfigure :inhaling 0.33ml/s @16:36:45.67 SS.seek 25ml @16:37:25.64 Vacuum too high -- waiting up to 40 seconds @16:37:25.65 SS.reconfigure :exhale @16:37:25.69 SS.hold @16:37:31.24 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:37:31.26 SS.reconfigure :inhaling 0.30ml/s @16:37:31.31 SS.seek 25ml @16:38:21.58 Stroke completed -- waiting for pressure to equalize ... @16:38:21.59 SS.reconfigure :exhale @16:38:21.63 SS.hold @16:38:37.83 Intake.select :close @16:38:37.97 SSV.dial :exhaust @16:38:38.72 Exhaust.select :open @16:38:38.83 SS.seek 0ml @16:38:47.92 Exhaust.select :close @16:38:48.03 SSV.dial CBV @16:38:48.05 CBV.dial SSV @16:38:48.80 Allowing 130s for 25.0ml of remaining 700.0ml @16:38:48.81 Intake.select :open @16:38:50.97 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:38:50.98 SS.reconfigure :inhaling 0.30ml/s @16:38:51.03 SS.seek 25ml @16:39:53.35 Vacuum too high -- waiting up to 40 seconds @16:39:53.36 SS.reconfigure :exhale @16:39:53.41 SS.hold @16:40:02.24 Intake @15.9psia, vacuum from 10.0 to 13.0psi @16:40:02.26 SS.reconfigure :inhaling 0.27ml/s @16:40:02.30 SS.seek 25ml @16:40:40.25 Stroke completed -- waiting for pressure to equalize ... @16:40:40.26 SS.reconfigure :exhale @16:40:40.30 SS.hold @16:40:58.54 Intake.select :close @16:40:58.69 SSV.dial :exhaust @16:40:59.44 Exhaust.select :open @16:40:59.55 SS.seek 0ml @16:41:09.00 Exhaust.select :close @16:41:09.11 SSV.dial CBV @16:41:09.13 CBV.dial SSV @16:41:09.89 Allowing 130s for 25.0ml of remaining 675.0ml @16:41:09.90 Intake.select :open @16:41:12.06 Intake @15.4psia, vacuum from 9.9 to 12.9psi @16:41:12.07 SS.reconfigure :inhaling 0.27ml/s @16:41:12.12 SS.seek 25ml @16:42:47.53 Vacuum too high -- waiting up to 40 seconds @16:42:47.55 SS.reconfigure :exhale @16:42:47.59 SS.hold @16:43:01.98 Intake @16.0psia, vacuum from 10.0 to 13.0psi @16:43:02.00 SS.reconfigure :inhaling 0.24ml/s @16:43:02.04 SS.seek 25ml @16:43:16.41 Stroke completed -- waiting for pressure to equalize ... @16:43:16.42 SS.reconfigure :exhale @16:43:16.47 SS.hold @16:43:19.91 SS.reconfigure :default @16:43:19.95 SS.hold @16:43:20.07 Intake.select :close @16:43:20.22 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 350.0ml @16:43:20.23 SSV.dial :exhaust @16:43:20.96 Exhaust.select :open @16:43:21.07 SS.reconfigure :exhale @16:43:21.11 SS.seek 0ml @16:43:30.54 Exhaust.select :close @16:43:30.65 Sampled 350.0ml @16:43:30.66 Drying puck before fixing for 20:00 @16:43:30.67 CBV.dial :waste2b @16:43:31.40 delay 1:00 @16:44:31.36 recoverPRVreagent08 @16:44:31.37 PTV.dial :mixing @16:44:31.38 PBV.dial :waste1 @16:44:31.42 CSV.dial :air @16:44:31.75 delay 30 seconds @16:44:31.85 CS.reconfigure :fast @16:44:31.90 CS.seek 10ml @16:44:55.35 Pumping wcr puck from tube 4 up to 35psi... @16:44:55.36 CBV.dial SSV @16:44:56.66 CBV.dial between CSV and waste2b @16:44:57.60 CSV.dial between air and CTV @16:44:57.92 CS.seek 7.278ml @16:45:01.76 PBV.dial between PSV and waste1 @16:45:02.14 PSV.dial :air @16:45:02.59 PS.reconfigure :fast @16:45:02.64 PS.seek 2.5ml @16:45:04.85 CTV.dial CSV,avoiding: :air @16:45:04.88 CSV.dial CTV @16:45:05.65 CS.reconfigure :pressurize @16:45:05.70 CS.seek 0ml @16:45:19.03 CBV.dial :waste2b @16:45:19.35 CS.reconfigure :default @16:45:19.40 CS.seek 0ml @16:45:25.59 delay 1:00 @16:45:26.02 PSV.dial :mixloop @16:45:26.48 delay 2 seconds @16:45:28.50 PSV.dial :air @16:45:28.95 delay 2 seconds @16:45:30.96 PSV.dial :mixloop @16:45:31.42 delay 2 seconds @16:45:33.43 PSV.dial :air @16:45:33.89 PS.seek 0ml @16:45:56.65 washCycleDA #1 of 3 @16:45:56.66 PSR.select :air @16:45:56.68 PSV.dial PSR @16:45:58.01 Pulling 0.5ml of PSR.wash @16:45:58.02 PSR.select :wash @16:45:58.14 PS.reconfigure :default @16:45:58.18 PS.seek 0.5ml @16:46:04.15 delay 5 seconds @16:46:09.17 PSR.select :air @16:46:09.28 PS.seek 2ml @16:46:25.27 PTV.dial :puck @16:46:25.62 CTV.dial :air,avoiding: :intake @16:46:26.01 PSV.dial PTV @16:46:26.73 PBV.dial :waste1 @16:46:26.91 Fix #1 of 2 (with RNAlater) for 10:00 @16:46:26.93 CSV.dial CSR @16:46:27.16 PS.seek 1.5ml @16:46:27.42 Pulling 2ml of CSR.RNAlater @16:46:27.43 CSR.select :RNAlater @16:46:27.54 CS.seek 2ml @16:46:33.09 delay 3 seconds @16:46:33.41 delay 5 seconds @16:46:36.10 PBV.dial between PSV and waste1 @16:46:36.47 PS.seek 0ml @16:46:38.42 CSR.select :air @16:46:38.54 CS.reconfigure :fast @16:46:38.58 CS.seek 10ml @16:46:52.49 delay 2:00 @16:46:57.16 Pumping wcr puck from tube 4 up to 35psi... @16:46:57.17 CBV.dial SSV @16:46:58.46 CBV.dial between CSV and waste2b @16:46:59.39 CSV.dial between air and CTV @16:46:59.99 CS.seek 7.134ml @16:47:07.22 CTV.dial CSV,avoiding: :air @16:47:07.25 CSV.dial CTV @16:47:08.60 CS.reconfigure :pressurize @16:47:08.65 CS.seek 0ml @16:47:25.11 delay 10:00 @16:48:52.52 recoverPSRreagent08 @16:48:52.53 PTV.dial :puck @16:48:52.54 PBV.dial :waste1 @16:48:52.91 delay 30 seconds @16:49:22.94 PBV.dial between PSV and waste1 @16:49:23.30 PSR.select :air @16:49:23.32 PSV.dial PSR @16:49:24.06 PS.reconfigure :fast @16:49:24.10 PS.seek 2.5ml @16:49:47.14 PSV.dial PTV @16:49:47.86 delay 5 seconds @16:49:52.88 PSV.dial PSR @16:49:53.61 delay 5 seconds @16:49:58.63 PSV.dial PTV @16:49:59.36 delay 5 seconds @16:50:04.38 PSV.dial :air @16:50:05.10 PS.seek 0ml @16:50:27.77 washCycleDA #2 of 3 @16:50:27.78 PSR.select :air @16:50:27.79 PSV.dial PSR @16:50:29.12 Pulling 0.5ml of PSR.wash @16:50:29.60 PSR.select :wash @16:50:29.72 PS.reconfigure :default @16:50:29.77 PS.seek 0.5ml @16:50:35.74 delay 5 seconds @16:50:40.76 PSR.select :air @16:50:40.87 PS.seek 2ml @16:50:56.86 PTV.dial :puck @16:50:56.87 PSV.dial PTV @16:50:57.70 PBV.dial :waste1 @16:50:58.14 PS.seek 1.5ml @16:51:04.02 delay 3 seconds @16:51:07.03 PBV.dial between PSV and waste1 @16:51:07.41 PS.seek 0ml @16:51:23.48 delay 2:00 @16:53:23.50 recoverPSRreagent08 @16:53:23.51 PTV.dial :puck @16:53:23.53 PBV.dial :waste1 @16:53:23.89 delay 30 seconds @16:53:53.91 PBV.dial between PSV and waste1 @16:53:54.28 PSR.select :air @16:53:54.30 PSV.dial PSR @16:53:55.02 PS.reconfigure :fast @16:53:55.06 PS.seek 2.5ml @16:54:18.04 PSV.dial PTV @16:54:18.78 delay 5 seconds @16:54:23.80 PSV.dial PSR @16:54:24.53 delay 5 seconds @16:54:29.55 PSV.dial PTV @16:54:30.28 delay 5 seconds @16:54:35.30 PSV.dial :air @16:54:36.02 PS.seek 0ml @16:54:58.73 washCycleDA #3 of 3 @16:54:58.74 PSR.select :air @16:54:58.75 PSV.dial PSR @16:55:00.08 Pulling 0.5ml of PSR.wash @16:55:00.09 PSR.select :wash @16:55:00.20 PS.reconfigure :default @16:55:00.24 PS.seek 0.5ml @16:55:06.07 delay 5 seconds @16:55:11.09 PSR.select :air @16:55:11.21 PS.seek 2ml @16:55:27.19 PTV.dial :puck @16:55:27.21 PSV.dial PTV @16:55:28.04 PBV.dial :waste1 @16:55:28.47 PS.seek 1.5ml @16:55:34.41 delay 3 seconds @16:55:37.43 PBV.dial between PSV and waste1 @16:55:37.81 PS.seek 0ml @16:55:53.85 delay 2:00 @16:57:25.13 CBV.dial :waste2b @16:57:25.46 CS.reconfigure :default @16:57:25.50 CS.seek 0ml @16:57:28.23 delay 1:00 @16:57:53.87 recoverPSRreagent08 @16:57:53.88 PTV.dial :puck @16:57:53.90 PBV.dial :waste1 @16:57:54.26 delay 30 seconds @16:58:24.28 PBV.dial between PSV and waste1 @16:58:24.65 PSR.select :air @16:58:24.66 PSV.dial PSR @16:58:25.38 PS.reconfigure :fast @16:58:25.43 PS.seek 2.5ml @16:58:28.25 CTV.dial :air,avoiding: :intake @16:58:29.54 Fix #2 of 2 (with RNAlater) for 10:00 @16:58:29.55 CSV.dial CSR @16:58:30.06 Pulling 2ml of CSR.RNAlater @16:58:30.07 CSR.select :RNAlater @16:58:30.18 CS.seek 2ml @16:58:36.01 delay 5 seconds @16:58:41.03 CSR.select :air @16:58:41.15 CS.reconfigure :fast @16:58:41.19 CS.seek 10ml @16:58:48.72 PSV.dial PTV @16:58:49.46 delay 5 seconds @16:58:54.48 PSV.dial PSR @16:58:55.33 delay 5 seconds @16:58:59.99 Pumping wcr puck from tube 4 up to 35psi... @16:59:00.00 CBV.dial SSV @16:59:00.35 PSV.dial PTV @16:59:01.07 delay 5 seconds @16:59:01.28 CBV.dial between CSV and waste2b @16:59:02.21 CSV.dial between air and CTV @16:59:02.82 CS.seek 7.024ml @16:59:06.08 PSV.dial :air @16:59:06.81 PS.seek 0ml @16:59:10.30 CTV.dial CSV,avoiding: :air @16:59:10.32 CSV.dial CTV @16:59:11.69 CS.reconfigure :pressurize @16:59:11.74 CS.seek 0ml @16:59:28.06 delay 10:00 @16:59:29.69 Gate.power :camera,:ON @16:59:29.70 Mixing DA Substrates @16:59:29.71 PSR.select :air @16:59:29.73 PSV.dial PSR @16:59:31.38 Pulling 0.25ml of PSR.sub1 @16:59:31.45 PSR.select :sub1 @16:59:31.73 PS.reconfigure :default @16:59:33.91 PS.seek 0.25ml @16:59:37.32 delay 5 seconds @16:59:42.38 Pulling 0.25ml of PSR.sub2 @16:59:42.39 PSR.select :sub2 @16:59:42.50 PS.seek 0.5ml @16:59:45.91 delay 5 seconds @16:59:50.93 PSR.select :air @16:59:51.04 PS.seek 2ml @17:00:07.12 PTV.dial :puck @17:00:07.14 PSV.dial PTV @17:00:07.86 PBV.dial :waste1 @17:00:08.30 PS.seek 1.5ml @17:00:14.16 delay 3 seconds @17:00:17.18 PBV.dial between PSV and waste1 @17:00:17.54 PS.seek 0ml @17:00:33.56 delay 10 seconds @17:00:43.59 recoverPSRreagent08 @17:00:43.60 PTV.dial :puck @17:00:43.61 PBV.dial :waste1 @17:00:43.95 delay 30 seconds @17:01:13.97 PBV.dial between PSV and waste1 @17:01:14.33 PSR.select :air @17:01:14.35 PSV.dial PSR @17:01:15.10 PS.reconfigure :fast @17:01:15.15 PS.seek 2.5ml @17:01:38.25 PSV.dial PTV @17:01:39.00 delay 5 seconds @17:01:44.02 PSV.dial PSR @17:01:44.75 delay 5 seconds @17:01:49.77 PSV.dial PTV @17:01:50.50 delay 5 seconds @17:01:55.52 PSV.dial :air @17:01:56.25 PS.seek 0ml @17:02:19.18 Additional air clear @17:02:19.19 PSR.select :air @17:02:19.21 PSV.dial PSR @17:02:20.55 Pulling 2ml of PSR.air @17:02:20.56 PSR.select :air @17:02:20.58 PS.reconfigure :default @17:02:20.62 PS.seek 2ml @17:02:41.90 delay 5 seconds @17:02:46.92 PTV.dial :puck @17:02:46.94 PSV.dial PTV @17:02:47.67 PBV.dial :waste1 @17:02:48.07 PS.seek 1.5ml @17:02:54.00 delay 3 seconds @17:02:57.01 PBV.dial between PSV and waste1 @17:02:57.38 delay 1 second @17:02:58.39 PSV.dial :waste1 @17:02:59.42 PS.seek 0ml @17:03:15.52 Processing Puck Heater at 27.8C before imaging @17:03:15.53 PH.coast @17:03:15.65 Imaging da puck in Processing Clamp while storing it to tube 3 @17:03:15.81 SC.reconfigure :tight @17:03:15.85 SC.seek 3 @17:03:19.42 SC.configure :limp @17:03:19.61 Elbow.reconfigure :tight @17:03:19.66 Elbow.seek 3 @17:03:21.36 Forearm.reconfigure :tight @17:03:21.40 Forearm.seek 3 + 700 counts @17:03:22.37 SE.reconfigure :pick @17:03:22.42 SE.seek :beamBreak @17:03:25.38 SE.configure :default @17:03:25.52 Elbow.reconfigure :limp @17:03:25.57 Forearm.reconfigure :loose @17:03:25.61 Forearm.seek 3 @17:03:26.01 Elbow.hold @17:03:26.07 Elbow.reconfigure :tight @17:03:26.16 SE.seek 3.2 Pucks below Top Plate @17:03:26.60 Forearm.force -20 @17:03:26.63 Forearm.seek 3 + 316 counts @17:03:26.92 Forearm.reconfigure :tight @17:03:26.97 Forearm.seek 3 + 700 counts @17:03:27.17 SE.seek 4.2 Pucks below Top Plate @17:03:27.66 Forearm.reconfigure :loose @17:03:27.71 Forearm.seek :retract @17:03:28.67 Elbow.seek PC @17:03:31.38 Elbow.reconfigure :loose @17:03:31.46 Forearm.seek PC @17:03:32.57 Hand.close @17:03:33.25 PC.seek :open @17:03:37.28 Forearm.seek :retract @17:03:38.33 Elbow.reconfigure :tight @17:03:38.37 Elbow.seek 3 @17:03:41.03 Forearm.reconfigure :tight @17:03:41.07 Forearm.seek 3 + 700 counts @17:03:42.10 Hand.open @17:03:42.93 SE.seek 3.84 Pucks below Top Plate @17:03:43.72 SE.reconfigure :seal @17:03:43.77 SE.seek 2.74 Pucks below Top Plate @17:03:44.33 Lights, camera, ACTION! @17:03:44.37 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23apr0617h252ml.tif @17:04:04.10 Waiting >20 seconds for da to relinquish FlushPuck @17:04:27.37 SX MX5/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 39.325 seconds /var/log/esp/da23apr0617h252ml.tif: TIFF Upload Complete @17:04:27.40 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23apr0617h252ml15s.tif @17:04:43.15 SX MX5/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23apr0617h252ml15s.tif: TIFF Upload Complete @17:04:43.18 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da23apr0617h252ml25s.tif @17:05:08.97 SX MX5/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da23apr0617h252ml25s.tif: TIFF Upload Complete @17:05:08.99 Gate.power :camera,:OFF @17:05:09.01 DAfans.email "39.325 sec auto-exposure",Subject: "da23apr0617h252ml images!" @17:05:09.34 Elbow.seek 3 @17:05:09.54 Forearm.seek 3 + 700 counts @17:05:09.62 SE.reconfigure :gently @17:05:09.68 SE.seek 3.45 Pucks below Top Plate @17:05:10.32 Elbow.reconfigure :limp @17:05:10.37 Forearm.reconfigure :loose @17:05:10.41 Forearm.seek 3 @17:05:10.88 Elbow.hold @17:05:10.95 Elbow.reconfigure :tight @17:05:11.01 SE.reconfigure :default @17:05:11.06 SE.seek 3.64 Pucks below Top Plate @17:05:11.54 Forearm.force -20 @17:05:11.57 Forearm.seek 3 + 300 counts @17:05:11.89 Forearm.reconfigure :tight @17:05:11.94 Forearm.seek 3 + 700 counts @17:05:12.39 SE.seek :down @17:05:13.66 Forearm.reconfigure :loose @17:05:13.74 Forearm.seek :retract @17:05:14.97 SE.stop @17:05:15.18 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:05:15.57 Elbow.seek FlushPuck::Garage @17:05:17.61 Elbow.reconfigure :loose @17:05:17.79 Forearm.seek :garage @17:05:18.96 Hand.close @17:05:19.87 Forearm.seek :retract @17:05:20.95 Elbow.reconfigure :tight @17:05:21.03 Elbow.seek PC @17:05:22.49 Elbow.reconfigure :loose @17:05:22.70 Forearm.reconfigure :tight @17:05:22.78 Forearm.seek PC @17:05:24.56 PC.seek :closed @17:05:28.55 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.38 @17:05:28.71 Hand.open @17:05:29.64 Forearm.reconfigure :loose @17:05:29.73 Forearm.seek :retract @17:05:30.78 PSR.select :air @17:05:30.81 PSV.dial PSR @17:05:31.45 Pulling 0.5ml of PSR.flush @17:05:31.47 PSR.select :flush @17:05:31.65 PS.seek 0.5ml @17:05:37.64 delay 5 seconds @17:05:42.67 PSR.select :air @17:05:42.86 PS.seek 2.5ml @17:05:43.43 upsync @17:06:03.99 delay 1 second @17:06:05.01 PRV.dial :waste2,avoiding: :ab2 @17:06:06.84 PTV.dial :PRVmixing @17:06:08.09 PSV.dial :mixloop @17:06:09.59 PS.seek :empty @17:06:35.83 PSR.select :air @17:06:35.85 PSV.dial PSR @17:06:36.92 Pulling 0.5ml of PSR.flush @17:06:36.93 PSR.select :flush @17:06:37.05 PS.seek 0.5ml @17:06:42.90 delay 5 seconds @17:06:47.92 PSR.select :air @17:06:48.03 PS.seek 2.5ml @17:07:09.12 delay 1 second @17:07:10.13 PBV.dial :waste1 @17:07:10.51 PTV.dial :mixing @17:07:11.21 PSV.dial :mixloop @17:07:12.23 PS.seek :empty @17:07:38.43 PSV.dial PSR @17:07:39.44 PSR.select :flush @17:07:39.56 PS.seek 1ml @17:07:50.52 PSR.select :air @17:07:50.63 PS.seek 2.5ml @17:08:06.68 PSV.dial PTV @17:08:07.41 PTV.dial :puck @17:08:08.13 PBV.dial :waste1 @17:08:08.15 PS.seek 0ml @17:08:34.27 PSV.dial PSR @17:08:35.00 PSR.select :flush @17:08:35.12 PS.seek 0.5ml @17:08:40.99 PSR.select :air @17:08:41.10 PS.seek 2.5ml @17:09:02.17 PSV.dial PTV @17:09:02.91 PBV.dial PSV @17:09:03.47 PS.seek 0ml @17:09:28.08 CBV.dial :waste2b @17:09:28.41 CS.reconfigure :default @17:09:28.45 CS.seek 0ml @17:09:29.61 PSV.dial PBV @17:09:30.07 PS.seek 2.5ml @17:09:31.06 delay 1:00 @17:09:56.20 PSV.dial :waste1 @17:09:56.93 PS.seek 0ml @17:10:23.17 PSV.dial PSR @17:10:23.62 PSR.select :air @17:10:23.65 PS.seek 2.5ml @17:10:31.08 CTV.dial :air,avoiding: :intake @17:10:32.41 Storing wcr puck in Collection Clamp to tube 3 @17:10:32.78 Elbow.reconfigure :tight @17:10:32.83 Elbow.seek 3 @17:10:35.53 Forearm.reconfigure :tight @17:10:35.58 Forearm.seek 3 + 700 counts @17:10:36.60 SE.reconfigure :pick @17:10:36.66 SE.seek :beamBreak @17:10:39.45 SE.configure :default @17:10:39.59 Elbow.reconfigure :limp @17:10:39.64 Forearm.reconfigure :loose @17:10:39.68 Forearm.seek 3 @17:10:40.11 Elbow.hold @17:10:40.17 Elbow.reconfigure :tight @17:10:40.27 SE.seek 4.2 Pucks below Top Plate @17:10:40.72 Forearm.force -20 @17:10:40.76 Forearm.seek 3 + 304 counts @17:10:41.05 Forearm.reconfigure :tight @17:10:41.10 Forearm.seek 3 + 700 counts @17:10:41.36 SE.seek 5.2 Pucks below Top Plate @17:10:41.94 Forearm.reconfigure :loose @17:10:41.99 Forearm.seek :retract @17:10:42.92 Elbow.seek CC @17:10:44.46 Elbow.reconfigure :loose @17:10:44.56 Forearm.seek CC @17:10:45.59 Hand.close @17:10:46.36 CC.seek :open @17:10:49.80 PSV.dial PTV @17:10:50.52 PBV.dial PSV @17:10:50.54 PS.seek 0ml @17:10:51.29 Forearm.seek :retract @17:10:52.31 Elbow.reconfigure :tight @17:10:52.36 Elbow.seek 3 @17:10:54.08 Elbow.reconfigure :limp @17:10:54.13 Forearm.seek 3 @17:10:55.23 Elbow.hold @17:10:55.30 Elbow.reconfigure :tight @17:10:55.35 Hand.open @17:10:56.23 Forearm.force -20 @17:10:56.27 Forearm.seek 3 + 319 counts @17:10:56.60 Forearm.reconfigure :tight @17:10:56.65 Forearm.seek 3 + 700 counts @17:10:56.94 SE.seek :down @17:10:58.07 Forearm.reconfigure :loose @17:10:58.11 Forearm.seek :retract @17:10:59.33 SE.stop @17:10:59.37
Starting finalPurge @17:11:16.70 PSV.dial PBV @17:11:17.16 PS.seek 2.5ml @17:11:19.27
Waiting >20 seconds for psFlush to relinquish FlushPuck @17:11:43.36 PSV.dial :waste1 @17:11:44.08 PS.seek 0ml @17:12:10.26 PSV.dial PSR @17:12:10.83 PSR.select :flush @17:12:10.94 PS.seek 1ml @17:12:21.98 PSR.select :air @17:12:22.09 PS.seek 2.5ml @17:12:38.23 PSV.dial PTV @17:12:38.97 PTV.dial :puck @17:12:38.98 PBV.dial :waste1 @17:12:39.52 PS.seek 0ml @17:13:05.64 PSV.dial PSR @17:13:06.37 PSR.select :flush @17:13:06.49 PS.seek 0.5ml @17:13:12.42 PSR.select :air @17:13:12.53 PS.seek 2.5ml @17:13:33.60 PSV.dial PTV @17:13:34.34 PBV.dial PSV @17:13:34.89 PS.seek 0ml @17:14:01.01 PSV.dial PBV @17:14:01.47 PS.seek 2.5ml @17:14:27.70 PSV.dial :waste1 @17:14:28.45 PS.seek 0ml @17:14:54.67 PSV.dial PSR @17:14:55.12 PSR.select :air @17:14:55.14 PS.seek 2.5ml @17:15:21.32 PSV.dial PTV @17:15:22.04 PBV.dial PSV @17:15:22.06 PS.seek 0ml @17:15:48.29 PSV.dial PBV @17:15:48.75 PS.seek 2.5ml @17:16:14.91 PSV.dial :waste1 @17:16:15.75 PS.seek 0ml @17:16:41.89
Loading FlushPuck into Collection Clamp from Processing Clamp @17:16:42.06 Elbow.seek PC @17:16:44.54 Elbow.reconfigure :loose @17:16:44.64 Forearm.seek PC @17:16:45.61 Hand.close @17:16:46.22 PC.seek :open @17:16:50.18 Forearm.seek :retract @17:16:51.28 Elbow.reconfigure :tight @17:16:51.33 Elbow.seek CC @17:16:54.91 Elbow.reconfigure :loose @17:16:55.00 Forearm.reconfigure :tight @17:16:55.04 Forearm.seek CC @17:16:56.26 CC.seek :closed @17:17:00.50 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.76 @17:17:00.57 Hand.open @17:17:01.40 Forearm.reconfigure :loose @17:17:01.45 Forearm.seek :retract @17:17:02.46 Flushing the Collection Valve::Manifold @17:17:02.58 CSV.dial CSR @17:17:03.07 Pulling 5ml of CSR.flush @17:17:03.08 CSR.select :flush @17:17:03.19 CS.seek 5ml @17:17:16.67 delay 5 seconds @17:17:21.69 CSR.select :air @17:17:21.84 CS.reconfigure :fast @17:17:21.88 CS.seek 7.5ml @17:17:28.26 CSV.dial :waste @17:17:29.53 CS.reconfigure :default @17:17:29.58 CS.seek 0ml @17:17:49.29 Flushing lower manifold's waste1 port @17:17:49.42 CSV.dial :flush @17:17:49.97 CS.reconfigure :fast @17:17:50.02 CS.seek 5ml @17:18:01.82 delay 5 seconds @17:18:06.84 CSV.dial :air @17:18:07.35 CS.seek 7ml @17:18:12.61 CSV.dial CTV @17:18:12.63
CTV.dial CSV @17:18:13.96 CBV.dial :waste1 @17:18:14.71 CS.seek 0ml @17:18:30.86 Flushing lower manifold's waste2 port @17:18:30.97 CSV.dial :flush @17:18:31.69 CS.seek 5ml @17:18:43.60 delay 5 seconds @17:18:48.62 CSV.dial :air @17:18:49.13 CS.seek 7ml @17:18:54.42 CSV.dial CTV @17:18:54.44
CTV.dial CSV @17:18:54.89 CBV.dial :waste2 @17:18:55.91 CS.seek 0ml @17:19:11.95 Flushing lower manifold's waste2b port @17:19:12.06 CSV.dial :flush @17:19:12.80 CS.seek 5ml @17:19:24.67 delay 5 seconds @17:19:29.68 CSV.dial :air @17:19:30.20 CS.seek 7ml @17:19:35.45 CSV.dial CTV @17:19:35.48
CTV.dial CSV @17:19:35.92 CBV.dial :waste2b @17:19:36.92 CS.seek 0ml @17:19:52.98 Disinfecting external Intake with kill @17:19:53.09 CSV.dial CSR @17:19:53.58 Pulling 8ml of CSR.kill @17:19:53.59 CSR.select :kill @17:19:53.70 CS.seek 8ml @17:20:12.17 delay 5 seconds @17:20:17.18 CSR.select :air @17:20:17.29 CS.seek 10ml @17:20:22.61 CSV.dial CBV @17:20:22.63
CBV.dial CSV @17:20:23.68 CTV.dial :intake @17:20:24.41 Intake.select :open @17:20:24.52 CS.seek 2ml @17:20:42.76 Intake.select :close @17:20:42.86 CTV.dial :air @17:20:43.59 CS.reconfigure :default @17:20:43.63 CS.seek 0ml @17:20:49.57 CSV.dial CSR @17:20:50.62 Pulling 5ml of CSR.flush @17:20:50.63 CSR.select :flush @17:20:50.74 CS.seek 5ml @17:21:04.26 delay 5 seconds @17:21:09.28 CSR.select :air @17:21:09.39 CS.seek 10ml @17:21:22.76 CSV.dial CBV @17:21:23.76 CS.seek 0ml @17:21:49.85 Garaging FlushPuck in Collection Clamp @17:21:49.92 Elbow.reconfigure :tight @17:21:49.96 Elbow.seek CC @17:21:50.08 Elbow.reconfigure :loose @17:21:50.16 Forearm.seek CC @17:21:51.18 Hand.close @17:21:51.96 CC.seek :open @17:21:56.30 Forearm.seek :retract @17:21:57.31 Elbow.reconfigure :tight @17:21:57.35 Elbow.seek :garage @17:22:00.33 Elbow.reconfigure :loose @17:22:00.41 Forearm.reconfigure :tight @17:22:00.46 Forearm.seek :garage @17:22:01.59 Hand.open @17:22:02.39 Forearm.reconfigure :loose @17:22:02.91 Forearm.seek :retract @17:22:03.93 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 15:24:08 @17:22:03.98 Asleep after completing DA_WCR_040623 assays @17:22:03.99 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 6,\n 4 => 27,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@17:13:11, 18.1C, 55% humidity, 15.5psia, 13.977V, 0.288A, 0.288A avg, 4.02W\nSampled 350.0ml",Subject: "Asleep after completing DA_WCR_040623 assays" @17:22:08.66 upsync @20:37:10.65UTC15-Apr-23
ESP2 v3.41 I2C Gateway v3.20 for core bus @20:37:13.22 Configuration for shorty 10/23/20 brent@mbari.org /home/esp/esp2/type/shallow/shorty/configure.rb @20:37:17.23 Polling Can every 10:00 @20:37:17.40 Cleanly exited at 20:19:10UTC15-Apr-23 @20:37:17.44 Loading /home/esp/esp2/mission/DA_WCR_041523.rb mission @20:37:17.49 Preparing to sleep until + 00:00:05 @20:37:17.50 GoodNews.email "Starting DA_WCR_041523 w/pucks in tube 4\n{1 => 33,\n 2 => 33,\n 3 => 6,\n 4 => 27,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:37:17, 23.4C, 60% humidity, 15.9psia, 13.963V, 0.214A, 0.208A avg, 2.99W",Subject: "DA_WCR_041523 begun" @20:37:17.55
Asleep til + 00:00:05 @20:37:17.59 Continuing at 20:37 @20:37:17.83 Gate.power :core,:OFF @20:37:17.85 Gate.power :raw,:OFF Shutdown @20:37:21.25 GoodNews.email "Then, in tube 4: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_041523.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 6,\n 4 => 27,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:37:17, 23.4C, 60% humidity, 15.9psia, 13.963V, 0.214A, 0.208A avg, 2.99W",Subject: "Asleep until 20:37" @20:37:22.53
Powering core bus @20:37:22.83 upsync @20:37:25.51 upsync @20:37:28.35
Gate.power :raw,:ON @20:37:29.39 CS.configure :default @20:37:29.48 PS.configure :default @20:37:29.56 SS.configure :default @20:37:29.66 CC.configure :default @20:37:29.75 PC.configure :default @20:37:29.86 Forearm.configure :loose @20:37:29.94 Elbow.configure :loose @20:37:30.05 SC.configure :loose @20:37:30.14 SE.configure :default @20:37:30.18 CH.coast @20:37:30.23 CH.configure :default @20:37:30.26 PH.coast @20:37:30.31 PH.configure :default @20:37:30.35 All dwarf controllers running firmware version 3.85 @20:37:30.45 Valve.reset @20:37:30.70 Intake.select :close @20:37:30.72 Exhaust.select :close @20:37:30.75 CTV.dial :air @20:37:30.84 PSV.dial :air @20:37:30.91 CSV.dial :waste1 @20:37:31.03 PS.reconfigure :default @20:37:31.06
Forearm.reconfigure :loose @20:37:31.14 Forearm.home.seek :retract @20:37:31.16 PS.home.seek 0ml @20:37:31.24 SSV.dial :spit,avoiding: :exhaust @20:37:31.37 CS.reconfigure :fast @20:37:31.40 SS.reconfigure :default @20:37:31.48 CS.home.seek 0ml @20:37:31.52 SS.home.seek 0ml @20:37:31.92 Hand.close @20:37:31.96
Elbow.jog 1500 @20:37:32.50 Elbow.reconfigure :loose @20:37:32.54 Elbow.home.seek :clear @20:37:36.99 SE.reconfigure :default @20:37:37.03 SE.home.seek :down @20:37:37.76 SC.reconfigure :tight @20:37:37.80 SC.home.seek :initial @20:37:39.80 PS.stop @20:38:07.84 I2C::Request::Timeout in MAIN -- No Response to I2C::Servo::AbsMove3Request[21:->28] @20:38:07.86
Retry #1 of 3 @20:38:07.91 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[21:28->20] aborted with status: @20:38:12.28 SC.configure :limp @20:38:12.33 Forearm.seek :retract @20:38:12.39 Elbow.seek :hammer @20:38:13.48 Forearm.reconfigure :tight @20:38:13.52 Forearm.seek retracted @20:38:13.95 PC.reconfigure :default @20:38:13.97
CC.reconfigure :default @20:38:14.04 PC.home.seek :open @20:38:14.07
CC.home.seek :open @20:38:17.30 Phase [initialPurge; da [100, 100] #da and wsr are run simultanelously] @20:38:17.31 Notice.email "At 20:38:17 began in tube 4: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_041523.rb:14\n{1 => 33,\n 2 => 33,\n 3 => 6,\n 4 => 27,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:38:17, 23.4C, 60% humidity, 15.9psia, 13.933V, 0.245A, 0.251A avg, 3.41W",Subject: "Started DA_WCR_041523 phase initialPurge; da [100, 100] #da and wsr are run simultanelously" @20:38:17.39
Starting initialPurge @20:38:17.40 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:38:17.48 Hand.open @20:38:18.38 Elbow.reconfigure :tight @20:38:18.43 Elbow.seek FlushPuck::Garage @20:38:20.42 Elbow.reconfigure :loose @20:38:20.63 Forearm.reconfigure :loose @20:38:20.71 Forearm.seek :garage @20:38:21.82 Hand.close @20:38:21.87 upsync @20:38:22.49
Forearm.seek :retract @20:38:23.58 Elbow.reconfigure :tight @20:38:23.63 Elbow.seek CC @20:38:26.50 Elbow.reconfigure :loose @20:38:26.59 Forearm.reconfigure :tight @20:38:26.63 Forearm.seek CC @20:38:27.82 CC.seek :closed @20:38:32.05 Collection Clamp onSpring/offSpring for 8/25 samples, on/off torque 1.87 @20:38:32.12 Hand.open @20:38:32.93 Forearm.reconfigure :loose @20:38:32.97 Forearm.seek :retract @20:38:34.03 Flushing the Collection Valve::Manifold @20:38:34.14 CSV.dial CSR @20:38:35.44 Pulling 5ml of CSR.flush @20:38:35.45 CSR.select :flush @20:38:35.56 CS.reconfigure :default @20:38:35.60 CS.seek 5ml @20:38:48.97 delay 5 seconds @20:38:53.99 CSR.select :air @20:38:54.14 CS.reconfigure :fast @20:38:54.18 CS.seek 7.5ml @20:39:00.62 CSV.dial :waste @20:39:01.88 CS.reconfigure :default @20:39:01.92 CS.seek 0ml @20:39:21.65 Purging external Intake @20:39:21.77 CSV.dial :flush @20:39:22.21 CS.reconfigure :fast @20:39:22.25 CS.seek 10ml @20:39:44.90 delay 5 seconds @20:39:49.92 CSV.dial CBV @20:39:49.95
CBV.dial CSV @20:39:50.81 CTV.dial :intake @20:39:51.53 Intake.select :open @20:39:51.63 CS.seek 0ml @20:40:14.21 Intake.select :close @20:40:14.31 CTV.dial :air @20:40:15.04 Flushing upper manifold @20:40:15.16 CSV.dial :flush @20:40:15.86 CS.seek 5ml @20:40:27.53 delay 5 seconds @20:40:32.54 CSV.dial :air @20:40:33.04 CS.seek 7ml @20:40:38.25 CSV.dial CBV @20:40:38.27
CBV.dial CSV @20:40:39.28 CTV.dial :air @20:40:39.30 CS.seek 0ml @20:40:55.28 delay 7 seconds @20:41:02.30 CS.seek 3ml @20:41:09.74 CSV.dial :waste @20:41:10.19 CS.seek 0ml @20:41:17.72 CSV.dial :flush @20:41:18.16 CS.seek 3ml @20:41:25.52 delay 5 seconds @20:41:30.54 CSV.dial :air @20:41:31.04 CS.seek 4.999ml @20:41:36.37 CSV.dial :air @20:41:36.39 CS.seek 0ml @20:41:48.15 delay 7 seconds @20:41:55.18 CS.seek 3ml @20:42:02.62 CSV.dial :waste @20:42:03.45 CS.seek 0ml @20:42:10.87 Garaging FlushPuck in Collection Clamp @20:42:10.94 Elbow.reconfigure :tight @20:42:10.98 Elbow.seek CC @20:42:11.09 Elbow.reconfigure :loose @20:42:11.17 Forearm.seek CC @20:42:12.19 Hand.close @20:42:12.86 CC.seek :open @20:42:17.20 Forearm.seek :retract @20:42:18.24 Elbow.reconfigure :tight @20:42:18.28 Elbow.seek :garage @20:42:21.23 Elbow.reconfigure :loose @20:42:21.31 Forearm.reconfigure :tight @20:42:21.35 Forearm.seek :garage @20:42:22.46 Hand.open @20:42:23.81 Forearm.reconfigure :loose @20:42:23.85 Forearm.seek :retract @20:42:24.96 da sampling at most 100ml, wcr at most 100ml @20:42:24.99 Loading sh1 puck from tube 4 into Collection Clamp @20:42:25.01 DAfans.email "Started da in tube 4 at 20:42:24UTC15-Apr-23\n{1 => 33,\n 2 => 33,\n 3 => 6,\n 4 => 27,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@20:38:17, 23.4C, 60% humidity, 15.9psia, 13.933V, 0.245A, 0.251A avg, 3.41W",Subject: "da sampling at most 100ml, wcr at most 100ml" @20:42:25.26
SC.reconfigure :tight @20:42:25.33 SC.seek 4 @20:42:28.86 upsync @20:42:32.03
SC.configure :limp @20:42:32.24 Elbow.reconfigure :tight @20:42:32.29 Elbow.seek 4 @20:42:34.24 Forearm.reconfigure :tight @20:42:34.29 Forearm.seek 4 + 700 counts @20:42:35.25 SE.reconfigure :pick @20:42:35.35 SE.seek :beamBreak @20:42:36.74 SE.configure :default @20:42:36.88 Elbow.seek 4 @20:42:37.05 Forearm.seek 4 + 300 counts @20:42:37.28 Forearm.reconfigure :loose @20:42:37.32 Hand.close @20:42:38.00 SE.seek :down @20:42:38.20
Forearm.seek :retract @20:42:39.19 SE.stop @20:42:39.24
Elbow.seek CC @20:42:40.89 Elbow.reconfigure :loose @20:42:40.98 Forearm.reconfigure :tight @20:42:41.02 Forearm.seek CC @20:42:42.31 CC.seek :closed @20:42:46.54 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.69 @20:42:46.55 Hand.open @20:42:47.29 Forearm.reconfigure :loose @20:42:47.33 Forearm.seek :retract @20:42:48.33 collectSample 100ml w/bubble point of 24psi @20:42:48.34 Priming 7ml @20:42:48.35 Intake.select :close @20:42:48.36 Exhaust.select :close @20:42:48.37 SS.hold @20:42:48.56 Calibrating pressure and temperature sensors @20:42:48.59 CTV.dial :air @20:42:48.61 SSV.dial :spit,avoiding: :exhaust @20:42:48.62 SS.reconfigure :fast @20:42:48.67 SS.seek 5ml @20:42:51.60 SSV.dial CBV @20:42:51.62
CBV.dial SSV @20:42:52.93 delay 3.8 seconds @20:42:56.84 SSV.dial :spit @20:42:58.10 SS.seek 0ml @20:43:00.86 SSV.dial CBV @20:43:02.13 CTV.dial :intake @20:43:02.96 SSV.dial CBV @20:43:02.99
CBV.dial SSV @20:43:03.00 Allowing 40s for 7.0ml of remaining 7.0ml @20:43:03.01 Intake.select :open @20:43:05.16 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:43:05.17 SS.reconfigure :inhaling 2.14ml/s @20:43:05.22 SS.seek 7ml @20:43:11.86 Stroke completed -- waiting for pressure to equalize ... @20:43:11.87 SS.reconfigure :exhale @20:43:11.91 SS.hold @20:43:14.32 Intake.select :close @20:43:14.46 SSV.dial :exhaust @20:43:15.19 Exhaust.select :open @20:43:15.30 SS.seek 0ml @20:43:19.62 Exhaust.select :close @20:43:19.73 Sampling 100.0ml @20:43:19.74 Intake.select :close @20:43:19.75 Exhaust.select :close @20:43:19.76 SS.reconfigure :default @20:43:19.80 SS.hold @20:43:19.98 CTV.dial :intake @20:43:19.99 SSV.dial CBV @20:43:20.02
CBV.dial SSV @20:43:20.77 Allowing 130s for 25.0ml of remaining 100.0ml @20:43:20.78 Intake.select :open @20:43:22.93 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:43:22.94 SS.reconfigure :inhaling 2.14ml/s @20:43:22.99 SS.seek 25ml @20:43:27.63 Vacuum too high -- waiting up to 40 seconds @20:43:27.64 SS.reconfigure :exhale @20:43:27.68 SS.hold @20:43:28.36 Intake @12.1psia, vacuum from 6.6 to 9.6psi @20:43:28.38 SS.reconfigure :inhaling 1.93ml/s @20:43:28.42 SS.seek 25ml @20:43:35.85 Vacuum too high -- waiting up to 40 seconds @20:43:36.25 SS.reconfigure :exhale @20:43:36.37 SS.hold @20:43:39.20 Intake @14.3psia, vacuum from 8.8 to 11.8psi @20:43:39.21 SS.reconfigure :inhaling 1.74ml/s @20:43:39.26 SS.seek 25ml @20:43:44.56 Stroke completed -- waiting for pressure to equalize ... @20:43:44.57 SS.reconfigure :exhale @20:43:44.61 SS.hold @20:43:46.16 Intake.select :close @20:43:46.30 SSV.dial :exhaust @20:43:47.03 Exhaust.select :open @20:43:47.14 SS.seek 0ml @20:43:56.44 Exhaust.select :close @20:43:56.54 SSV.dial CBV @20:43:56.56
CBV.dial SSV @20:43:57.31 Allowing 130s for 25.0ml of remaining 75.0ml @20:43:57.32 Intake.select :open @20:43:59.48 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:43:59.49 SS.reconfigure :inhaling 1.74ml/s @20:43:59.53 SS.seek 25ml @20:44:04.79 Vacuum too high -- waiting up to 40 seconds @20:44:04.80 SS.reconfigure :exhale @20:44:04.84 SS.hold @20:44:05.43 Intake @12.2psia, vacuum from 6.7 to 9.7psi @20:44:05.44 SS.reconfigure :inhaling 1.57ml/s @20:44:05.49 SS.seek 25ml @20:44:18.75 Stroke completed -- waiting for pressure to equalize ... @20:44:18.76 SS.reconfigure :exhale @20:44:18.80 SS.hold @20:44:21.56 Intake.select :close @20:44:21.70 SSV.dial :exhaust @20:44:22.44 Exhaust.select :open @20:44:22.54 SS.seek 0ml @20:44:31.53 Exhaust.select :close @20:44:31.63 SSV.dial CBV @20:44:31.66
CBV.dial SSV @20:44:32.41 Allowing 130s for 25.0ml of remaining 50.0ml @20:44:32.42 Intake.select :open @20:44:34.57 Intake @15.9psia, vacuum from 10.0 to 13.0psi @20:44:34.58 SS.reconfigure :inhaling 1.57ml/s @20:44:34.63 SS.seek 25ml @20:44:42.97 Vacuum too high -- waiting up to 40 seconds @20:44:42.98 SS.reconfigure :exhale @20:44:43.02 SS.hold @20:44:43.57 Intake @12.4psia, vacuum from 6.9 to 9.9psi @20:44:43.58 SS.reconfigure :inhaling 1.41ml/s @20:44:43.63 SS.seek 25ml @20:44:55.91 Stroke completed -- waiting for pressure to equalize ... @20:44:55.92 SS.reconfigure :exhale @20:44:55.96 SS.hold @20:44:58.14 Intake.select :close @20:44:58.28 SSV.dial :exhaust @20:44:59.00 Exhaust.select :open @20:44:59.10 SS.seek 0ml @20:45:08.40 Exhaust.select :close @20:45:08.50 SSV.dial CBV @20:45:08.54
CBV.dial SSV @20:45:09.28 Allowing 130s for 25.0ml of remaining 25.0ml @20:45:09.29 Intake.select :open @20:45:11.44 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:45:11.46 SS.reconfigure :inhaling 1.41ml/s @20:45:11.50 SS.seek 25ml @20:45:25.87 Vacuum too high -- waiting up to 40 seconds @20:45:25.89 SS.reconfigure :exhale @20:45:25.97 SS.hold @20:45:26.36 Intake @12.5psia, vacuum from 7.0 to 10.0psi @20:45:26.37 SS.reconfigure :inhaling 1.27ml/s @20:45:26.41 SS.seek 25ml @20:45:33.10 Stroke completed -- waiting for pressure to equalize ... @20:45:33.11 SS.reconfigure :exhale @20:45:33.15 SS.hold @20:45:34.34 Intake.select :close @20:45:34.48 SSV.dial :exhaust @20:45:35.21 Exhaust.select :open @20:45:35.32 SS.seek 0ml @20:45:44.34 Exhaust.select :close @20:45:44.44 Sampled 100.0ml @20:45:44.46 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @20:45:44.47 Intake.select :close @20:45:44.52 CSV.dial :waste @20:45:44.56 CS.seek 0ml @20:45:44.67 CSV.dial :air @20:45:44.69 SSV.dial CBV,avoiding: :exhaust @20:45:44.72
CBV.dial SSV @20:45:45.43 CS.seek 10ml @20:45:46.58
CTV.dial :air @20:45:47.28 delay 15 seconds @20:46:02.30 SSV.dial :spit,avoiding: :exhaust @20:46:03.61 CTV.dial CSV,avoiding: :intake @20:46:03.65
SS.seek 0ml @20:46:03.76 SSV.dial CBV,avoiding: :exhaust @20:46:08.33 CBV.dial between SSV and waste2 @20:46:08.69 CTV.dial CSV,avoiding: :air @20:46:08.71
CSV.dial CTV @20:46:09.15 CS.reconfigure :pressurize @20:46:09.20 CS.seek 0ml @20:46:27.21 BubblePoint: Intake @40.4psia, Outlet @16.2psia @20:46:27.22 CBV.dial SSV @20:46:27.52 SS.reconfigure :default @20:46:27.57 SS.seek 10ml @20:46:33.40 delay 15 seconds @20:46:48.52 Evacuated: Intake @16.4psia, Outlet @10.4psia @20:46:48.53 SSV.dial :spit,avoiding: :exhaust @20:46:49.89 SS.reconfigure :exhale @20:46:49.94 SS.seek 0ml @20:46:55.46 SS.reconfigure :fast @20:46:55.50 SS.seek 5ml @20:46:58.60 SSV.dial CBV,avoiding: :exhaust @20:46:58.64
CBV.dial SSV @20:46:59.94 CTV.dial :air,avoiding: :intake @20:47:01.21 CSV.dial CTV @20:47:01.24
SS.seek 10ml @20:47:02.24 CSV.dial :air @20:47:02.69 CS.reconfigure :fast @20:47:02.74 CS.seek 0ml @20:47:03.96
SSV.dial :spit,avoiding: :exhaust @20:47:05.27 SS.reconfigure :exhale @20:47:05.31 SS.seek 0ml @20:47:10.23 CS.stop @20:47:10.57
SS.reconfigure :fast @20:47:10.61 SS.seek 5ml @20:47:13.43 SSV.dial CBV,avoiding: :exhaust @20:47:13.46
CBV.dial SSV @20:47:15.78 SSV.dial :spit,avoiding: :exhaust @20:47:17.09 SS.reconfigure :default @20:47:17.13 SS.seek 0ml @20:47:20.46 SS.stop @20:47:20.50 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @20:47:20.57 CSV.dial :waste @20:47:20.69 SSV.dial CBV,avoiding: :exhaust @20:47:20.71
CBV.dial SSV @20:47:21.35 CS.seek 0ml @20:47:21.42 CSV.dial :air @20:47:22.01
CTV.dial :air @20:47:22.02 delay 15 seconds @20:47:22.13 CS.seek 10ml @20:47:37.04
SSV.dial :spit,avoiding: :exhaust @20:47:38.34 CTV.dial CSV,avoiding: :intake @20:47:38.39
SS.reconfigure :exhale @20:47:38.46 SS.seek 0ml @20:47:38.96 SSV.dial CBV,avoiding: :exhaust @20:47:44.85 CBV.dial between SSV and waste2 @20:47:45.22 CTV.dial CSV,avoiding: :air @20:47:45.24
CSV.dial CTV @20:47:45.68 CS.reconfigure :pressurize @20:47:45.72 CS.seek 0ml @20:48:06.70 BubblePoint: Intake @39.5psia, Outlet @15.3psia @20:48:06.71 CBV.dial SSV @20:48:07.03 SS.reconfigure :default @20:48:07.08 SS.seek 10ml @20:48:12.73 delay 15 seconds @20:48:27.84 Evacuated: Intake @18.1psia, Outlet @12.5psia @20:48:27.85 SSV.dial :spit,avoiding: :exhaust @20:48:29.15 SS.reconfigure :exhale @20:48:29.20 SS.seek 0ml @20:48:34.67 SS.reconfigure :fast @20:48:34.71 SS.seek 5ml @20:48:37.51 SSV.dial CBV,avoiding: :exhaust @20:48:37.53
CBV.dial SSV @20:48:38.84 CTV.dial :air,avoiding: :intake @20:48:40.09 CSV.dial CTV @20:48:40.13
SS.seek 10ml @20:48:41.12 CSV.dial :air @20:48:41.58 CS.reconfigure :fast @20:48:41.63 CS.seek 0ml @20:48:42.85
SSV.dial :spit,avoiding: :exhaust @20:48:44.17 SS.reconfigure :exhale @20:48:44.21 SS.seek 0ml @20:48:46.54 CS.stop @20:48:49.64
SS.reconfigure :fast @20:48:49.68 SS.seek 5ml @20:48:52.35 SSV.dial CBV,avoiding: :exhaust @20:48:52.39
CBV.dial SSV @20:48:54.69 SSV.dial :spit,avoiding: :exhaust @20:48:55.98 SS.reconfigure :default @20:48:56.02 SS.seek 0ml @20:48:59.38 SS.stop @20:48:59.42 Lysing: Adding 2.0ml AqMeOH @20:48:59.43 CBV.dial between CSV and waste1,avoiding: :DBV @20:49:00.89 CTV.dial CSV @20:49:02.14 CSV.dial CSR @20:49:03.24 Pulling 1ml of CSR.AqMeOH @20:49:03.25 CSR.select :AqMeOH @20:49:03.36 CS.reconfigure :default @20:49:03.40 CS.seek 1ml @20:49:06.73 delay 5 seconds @20:49:11.75 CSR.select :air @20:49:11.90 CS.seek 6ml @20:49:25.39 delay 2 seconds @20:49:27.40 CSV.dial :waste2 @20:49:27.84 CS.reconfigure :fast @20:49:27.89 CS.seek 0ml @20:49:41.86 CSV.dial CSR @20:49:42.36 Pulling 2ml of CSR.AqMeOH @20:49:42.37 CSR.select :AqMeOH @20:49:42.48 CS.reconfigure :default @20:49:42.52 CS.seek 2ml @20:49:48.48 delay 5 seconds @20:49:53.50 CSR.select :air @20:49:53.65 CS.seek 5.001ml @20:50:02.11 delay 2 seconds @20:50:04.12 CSV.dial CTV @20:50:04.55 CS.seek 1ml @20:50:16.42 Before lysing Collection Puck Heater at 20.3C/38.5psia @20:50:16.43 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @20:50:16.45 CH.seek 70C,8:00 @20:52:02.72 CH.hold 10:00,70C @20:52:02.90 Begun lysing Collection Puck Heater at 68C/42.5psia @21:02:02.91 Ended lysing Collection Puck Heater at 70C/42.3psia @21:02:02.92 CS.seek 0ml @21:02:06.43 CTV.dial between spare and air,avoiding: :intake @21:02:07.41 CSV.dial :air @21:02:07.86 CS.seek 5ml @21:02:21.24 CSV.dial CTV @21:02:21.80 delay 2 seconds @21:02:23.82 CSV.dial :air @21:02:24.24 delay 2 seconds @21:02:26.26 CSV.dial CTV @21:02:26.69 delay 2 seconds @21:02:28.71 CSV.dial :air @21:02:29.13 delay 2 seconds @21:02:31.15 CS.seek 0ml @21:02:44.66 Puck @64.7psia after final lysis pressurization @21:02:44.68 Recovering Lysate into Collection syringe @21:02:44.69 CSV.dial CBV @21:02:45.68 CBV.dial CSV @21:02:46.03 CS.seek 4.999ml @21:02:59.40 delay 5 seconds @21:03:04.42 CTV.dial :air @21:03:04.91 CS.seek 6.999ml @21:03:10.71 delay 5 seconds @21:03:15.72 CSV.dial :air2 @21:03:16.15 CS.seek 10ml @21:03:24.46 CSV.dial between air and CTV,avoiding: CBV @21:03:25.70 Lysing Complete @21:03:25.76 Storing sh1 puck in Collection Clamp to tube 3 @21:03:25.92 SC.reconfigure :tight @21:03:25.96 SC.seek 3 @21:03:29.52 SC.configure :limp @21:03:29.71 Elbow.reconfigure :tight @21:03:29.75 Elbow.seek 3 @21:03:31.57 Forearm.reconfigure :tight @21:03:31.61 Forearm.seek 3 + 700 counts @21:03:32.72 SE.reconfigure :pick @21:03:32.77 SE.seek :beamBreak @21:03:35.56 SE.configure :default @21:03:35.70 Elbow.reconfigure :limp @21:03:35.75 Forearm.reconfigure :loose @21:03:35.78 Forearm.seek 3 @21:03:36.18 Elbow.hold @21:03:36.23 Elbow.reconfigure :tight @21:03:36.32 SE.seek 5.22 Pucks below Top Plate @21:03:36.78 Forearm.force -20 @21:03:36.81 Forearm.seek 3 + 308 counts @21:03:37.18 Forearm.reconfigure :tight @21:03:37.22 Forearm.seek 3 + 700 counts @21:03:37.51 SE.seek 6.22 Pucks below Top Plate @21:03:38.05 Forearm.reconfigure :loose @21:03:38.09 Forearm.seek :retract @21:03:38.99 Elbow.seek CC @21:03:40.67 Elbow.reconfigure :loose @21:03:40.76 Forearm.seek CC @21:03:41.71 Hand.close @21:03:42.44 CC.seek :open @21:03:46.89 Forearm.seek :retract @21:03:47.93 Elbow.reconfigure :tight @21:03:47.97 Elbow.seek 3 @21:03:49.78 Elbow.reconfigure :limp @21:03:49.83 Forearm.seek 3 @21:03:50.96 Elbow.hold @21:03:51.02 Elbow.reconfigure :tight @21:03:51.06 Hand.open @21:03:52.43 Forearm.force -20 @21:03:52.47 Forearm.seek 3 + 317 counts @21:03:52.79 Forearm.reconfigure :tight @21:03:52.84 Forearm.seek 3 + 700 counts @21:03:53.25 SE.seek :down @21:03:54.38
Forearm.reconfigure :loose @21:03:54.43 Forearm.seek :retract @21:03:55.54 SE.stop @21:03:55.58
Tranferring DA @21:03:55.59 Beginning samplePassC @21:03:55.60 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:03:55.79 Elbow.seek FlushPuck::Garage @21:03:57.76 Elbow.reconfigure :loose @21:03:57.85 Forearm.seek :garage @21:03:59.00 Hand.close @21:03:59.68 Forearm.seek :retract @21:04:00.83 Elbow.reconfigure :tight @21:04:00.88 Elbow.seek CC @21:04:03.75 Elbow.reconfigure :loose @21:04:03.83 Forearm.reconfigure :tight @21:04:03.87 Forearm.seek CC @21:04:05.17 CC.seek :closed @21:04:09.38 Collection Clamp onSpring/offSpring for 8/26 samples, on/off torque 1.92 @21:04:09.45 Hand.open @21:04:10.13 Forearm.reconfigure :loose @21:04:10.16 Forearm.seek :retract @21:04:11.17 PSV.dial PSR @21:04:12.50 Pulling 1.5ml of PSR.flush @21:04:12.51 PSR.select :flush @21:04:12.62 PS.seek 1.5ml @21:04:28.70 delay 5 seconds @21:04:33.72 PSV.dial :waste1 @21:04:34.17 PS.seek 0ml @21:04:50.22 PSV.dial PSR @21:04:50.66 PSR.select :air @21:04:50.77 PS.seek 2.5ml @21:05:17.01 PRV.dial CBV,avoiding: :ab2 @21:05:17.03
CBV.dial PRV @21:05:18.68 PSV.dial :mixloop @21:05:19.68 CTV.dial :air,avoiding: :intake @21:05:19.69 PTV.dial :PRVmixing @21:05:20.93 PS.seek 0ml @21:05:47.13 PSV.dial :air @21:05:47.56 PS.seek 2.5ml @21:06:13.72 PSV.dial :mixloop @21:06:14.17 PS.seek 0ml @21:06:40.38 PSV.dial :air @21:06:40.82 PS.seek 2.5ml @21:07:07.03 PSV.dial :mixloop @21:07:07.48 PS.seek 0ml @21:07:33.72 Garaging FlushPuck in Collection Clamp @21:07:33.79 Elbow.reconfigure :tight @21:07:33.83 Elbow.seek CC @21:07:33.94 Elbow.reconfigure :loose @21:07:34.04 Forearm.seek CC @21:07:35.09 Hand.close @21:07:35.81 CC.seek :open @21:07:40.15 Forearm.seek :retract @21:07:41.19 Elbow.reconfigure :tight @21:07:41.23 Elbow.seek :garage @21:07:44.11 Elbow.reconfigure :loose @21:07:44.20 Forearm.reconfigure :tight @21:07:44.24 Forearm.seek :garage @21:07:45.31 Hand.open @21:07:46.12 Forearm.reconfigure :loose @21:07:46.16 Forearm.seek :retract @21:07:47.20 PSV.dial :mixloop @21:07:47.67 PTV.dial :PRVmixing @21:07:47.69 PRV.dial CBV @21:07:47.71
CBV.dial PRV @21:07:47.73 CTV.dial :air,avoiding: :intake @21:07:47.75 Loading lyfil puck from tube 4 into Collection Clamp @21:07:47.97 SC.reconfigure :tight @21:07:48.01 SC.seek 4 @21:07:51.60 SC.configure :limp @21:07:51.79 Elbow.reconfigure :tight @21:07:51.84 Elbow.seek 4 @21:07:53.81 Forearm.reconfigure :tight @21:07:53.86 Forearm.seek 4 + 700 counts @21:07:54.84 SE.reconfigure :pick @21:07:54.89 SE.seek :beamBreak @21:07:56.35 SE.configure :default @21:07:56.49 Elbow.seek 4 @21:07:56.76 Forearm.seek 4 + 300 counts @21:07:57.04 Forearm.reconfigure :loose @21:07:57.09 Hand.close @21:07:57.70 SE.seek :down @21:07:57.88
Forearm.seek :retract @21:07:58.81 SE.stop @21:07:58.87
Elbow.seek CC @21:08:00.46 Elbow.reconfigure :loose @21:08:00.54 Forearm.reconfigure :tight @21:08:00.59 Forearm.seek CC @21:08:01.84 CC.seek :closed @21:08:06.02 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.63 @21:08:06.03 Hand.open @21:08:06.87 Forearm.reconfigure :loose @21:08:06.91 Forearm.seek :retract @21:08:07.95 CTV.dial CSV,avoiding: :intake @21:08:09.22 CSV.dial CTV @21:08:09.52 PS.seek 2.5ml @21:08:35.84 CS.seek 5.499ml @21:08:48.08 delay 8 seconds @21:08:56.10 PSV.dial :air @21:08:56.55 delay 2 seconds @21:08:58.56 PSV.dial :waste2 @21:08:59.29 PS.seek 0ml @21:09:25.47 CBV.dial between PRV and waste1 @21:09:25.79 CSV.dial :air @21:09:26.25 CS.seek 10ml @21:09:38.45 CSV.dial CTV @21:09:39.00 CS.seek 0ml @21:10:05.13 CBV.dial :waste1 @21:10:05.43 delay 5 seconds @21:10:10.45 CTV.dial :air,avoiding: :intake @21:10:11.75 Storing lyfil puck in Collection Clamp to tube 3 @21:10:11.91 SC.reconfigure :tight @21:10:11.95 SC.seek 3 @21:10:15.54 SC.configure :limp @21:10:15.73 Elbow.reconfigure :tight @21:10:15.77 Elbow.seek 3 @21:10:17.59 Forearm.reconfigure :tight @21:10:17.63 Forearm.seek 3 + 700 counts @21:10:18.72 SE.reconfigure :pick @21:10:18.77 SE.seek :beamBreak @21:10:21.46 SE.configure :default @21:10:21.60 Elbow.reconfigure :limp @21:10:21.64 Forearm.reconfigure :loose @21:10:21.68 Forearm.seek 3 @21:10:22.10 Elbow.hold @21:10:22.62 Elbow.reconfigure :tight @21:10:22.71 SE.seek 6.23 Pucks below Top Plate @21:10:23.24 Forearm.force -20 @21:10:23.28 Forearm.seek 3 + 319 counts @21:10:23.61 Forearm.reconfigure :tight @21:10:23.65 Forearm.seek 3 + 700 counts @21:10:23.89 SE.seek 7.23 Pucks below Top Plate @21:10:24.41 Forearm.reconfigure :loose @21:10:24.45 Forearm.seek :retract @21:10:25.37 Elbow.seek CC @21:10:26.99 Elbow.reconfigure :loose @21:10:27.12 Forearm.seek CC @21:10:28.11 Hand.close @21:10:28.68 CC.seek :open @21:10:33.12 Forearm.seek :retract @21:10:34.18 Elbow.reconfigure :tight @21:10:34.22 Elbow.seek 3 @21:10:36.00 Elbow.reconfigure :limp @21:10:36.05 Forearm.seek 3 @21:10:37.19 Elbow.hold @21:10:37.25 Elbow.reconfigure :tight @21:10:37.30 Hand.open @21:10:38.17 Forearm.force -20 @21:10:38.21 Forearm.seek 3 + 318 counts @21:10:38.49 Forearm.reconfigure :tight @21:10:38.54 Forearm.seek 3 + 700 counts @21:10:38.97 SE.seek :down @21:10:40.10
Forearm.reconfigure :loose @21:10:40.14 Forearm.seek :retract @21:10:41.30 SE.stop @21:10:41.33
PSV.dial :air @21:10:42.05 PS.seek 2.5ml @21:11:08.34 delay 1 second @21:11:09.35 PSV.dial :mixloop @21:11:09.78 delay 1 second @21:11:10.79 PSV.dial :air @21:11:11.22 delay 1 second @21:11:12.23 PSV.dial :mixloop @21:11:12.68 delay 1 second @21:11:13.70 PSV.dial :air @21:11:14.14 delay 1 second @21:11:15.16 PS.seek 0ml @21:11:41.31 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @21:11:41.48 Elbow.seek FlushPuck::Garage @21:11:43.37 Elbow.reconfigure :loose @21:11:43.46 Forearm.seek :garage @21:11:44.57 Hand.close @21:11:45.29 Forearm.seek :retract @21:11:46.38 Elbow.reconfigure :tight @21:11:46.42 Elbow.seek PC @21:11:47.70 Elbow.reconfigure :loose @21:11:47.78 Forearm.reconfigure :tight @21:11:47.83 Forearm.seek PC @21:11:49.17 PC.seek :closed @21:11:53.03 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.39 @21:11:53.09 Hand.open @21:11:53.73 Forearm.reconfigure :loose @21:11:53.78 Forearm.seek :retract @21:11:54.80 PRV.dial :air @21:11:55.24 PSV.dial :mixloop @21:11:55.76 PS.seek 0.3ml @21:12:00.04 PSV.dial PSR @21:12:01.08 Pulling 0.4ml of PSR.air @21:12:01.09 PSR.select :air @21:12:01.12 PS.seek 0.7ml @21:12:05.93 delay 3 seconds @21:12:08.95 PSR.select :air @21:12:08.97 PS.seek 2.5ml @21:12:28.06 delay 3 seconds @21:12:31.07 PSV.dial :mixloop @21:12:32.06 PTV.dial :mixing @21:12:32.78 PBV.dial :waste2 @21:12:33.67 PS.seek 0ml @21:12:59.93 Garaging FlushPuck in Processing Clamp @21:13:00.00 Elbow.reconfigure :tight @21:13:00.04 Elbow.seek PC @21:13:00.15 Elbow.reconfigure :loose @21:13:00.24 Forearm.seek PC @21:13:01.19 Hand.close @21:13:01.80 PC.seek :open @21:13:05.76 Forearm.seek :retract @21:13:06.77 Elbow.reconfigure :tight @21:13:06.81 Elbow.seek :garage @21:13:08.43 Elbow.reconfigure :loose @21:13:08.52 Forearm.reconfigure :tight @21:13:08.56 Forearm.seek :garage @21:13:09.67 Hand.open @21:13:10.48 Forearm.reconfigure :loose @21:13:10.52 Forearm.seek :retract @21:13:11.61 Loading da puck from tube 4 into Processing Clamp @21:13:11.83 SC.reconfigure :tight @21:13:11.87 SC.seek 4 @21:13:15.45 SC.configure :limp @21:13:15.64 Elbow.reconfigure :tight @21:13:15.68 Elbow.seek 4 @21:13:17.66 Forearm.reconfigure :tight @21:13:17.70 Forearm.seek 4 + 700 counts @21:13:18.75 SE.reconfigure :pick @21:13:18.80 SE.seek :beamBreak @21:13:20.27 SE.configure :default @21:13:20.41 Elbow.seek 4 @21:13:20.58 Forearm.seek 4 + 300 counts @21:13:20.84 Forearm.reconfigure :loose @21:13:20.88 Hand.close @21:13:21.56 SE.seek :down @21:13:21.75
Forearm.seek :retract @21:13:22.74 SE.stop @21:13:22.80
Elbow.seek PC @21:13:25.34 Elbow.reconfigure :loose @21:13:25.42 Forearm.reconfigure :tight @21:13:25.47 Forearm.seek PC @21:13:26.85 PC.seek :closed @21:13:30.75 Processing Clamp onSpring/offSpring for 9/23 samples, on/off torque 1.37 @21:13:30.76 Hand.open @21:13:31.60 Forearm.reconfigure :loose @21:13:31.64 Forearm.seek :retract @21:13:32.70 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @21:13:32.75 PH.seek 28C,5:00 @21:14:17.89 PH.hold 2:00:00,28C @21:14:18.42 delay 1:30 @21:15:48.46 PSR.select :air @21:15:48.47 PSV.dial PSR @21:15:49.53 Pulling 0.5ml of PSR.wash @21:15:49.54 PSR.select :wash @21:15:49.65 PS.seek 0.5ml @21:15:55.62 delay 5 seconds @21:16:00.63 PSR.select :air @21:16:00.75 PS.seek 2ml @21:16:16.80 PTV.dial :puck @21:16:17.50 PSV.dial PTV @21:16:18.22 PBV.dial :waste1 @21:16:19.53 PS.seek 1.5ml @21:16:25.39 delay 3 seconds @21:16:28.41 PBV.dial between PSV and waste1 @21:16:28.76 PS.seek 0ml @21:16:44.86 delay 5:00 @21:21:44.88 recoverPSRreagent08 @21:21:44.89 PTV.dial :puck @21:21:44.90 PBV.dial :waste1 @21:21:45.26 delay 30 seconds @21:22:15.28 PBV.dial between PSV and waste1 @21:22:15.64 PSR.select :air @21:22:15.65 PSV.dial PSR @21:22:16.37 PS.reconfigure :fast @21:22:16.42 PS.seek 2.5ml @21:22:39.13 PSV.dial PTV @21:22:39.85 delay 5 seconds @21:22:44.87 PSV.dial PSR @21:22:45.59 delay 5 seconds @21:22:50.61 PSV.dial PTV @21:22:51.32 delay 5 seconds @21:22:56.34 PSV.dial :air @21:22:57.06 PS.seek 0ml @21:23:19.60 PTV.dial :PRVmixing,via: PRV @21:23:20.85 PSV.dial :mixloop @21:23:21.30 PRV.dial :ab1 @21:23:22.03 PS.reconfigure :slow @21:23:22.07 PS.seek 0.15ml @21:23:25.57 delay 2 seconds @21:23:27.59 CBV.dial PRV @21:23:27.61
PRV.dial CBV @21:23:28.18 PS.seek 0.34ml @21:23:32.48 delay 2 seconds @21:23:34.49 CBV.dial between PRV and PSV @21:23:34.80 PRV.dial :air @21:23:35.25 PS.seek 0.9ml @21:23:47.07 PRV.dial :waste2 @21:23:47.78 PS.seek 0.5ml @21:23:56.35 addPMLReagent08 @21:23:56.36 PSV.dial :air @21:23:56.81 PS.reconfigure :default @21:23:56.85 PS.seek 2ml @21:24:12.85 PSV.dial :PML @21:24:13.29 PTV.dial :mixing @21:24:14.02 PBV.dial :waste1 @21:24:14.43 PS.seek 1.85ml @21:24:16.72 PBV.dial between PSV and waste1 @21:24:17.09 PS.seek 0ml @21:24:36.76 delay 2 seconds @21:24:38.77 Beginning samplePassC @21:24:38.79 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:24:38.95 Elbow.reconfigure :tight @21:24:38.99 Elbow.seek FlushPuck::Garage @21:24:40.36 Elbow.reconfigure :loose @21:24:40.45 Forearm.seek :garage @21:24:41.57 Hand.close @21:24:42.26 Forearm.seek :retract @21:24:43.40 Elbow.reconfigure :tight @21:24:43.45 Elbow.seek CC @21:24:46.28 Elbow.reconfigure :loose @21:24:46.37 Forearm.reconfigure :tight @21:24:46.42 Forearm.seek CC @21:24:47.67 CC.seek :closed @21:24:51.91 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.79 @21:24:51.98 Hand.open @21:24:53.18 Forearm.reconfigure :loose @21:24:53.22 Forearm.seek :retract @21:24:54.21 PSV.dial PSR @21:24:55.26 Pulling 1.5ml of PSR.flush @21:24:55.27 PSR.select :flush @21:24:55.39 PS.seek 1.5ml @21:25:11.44 delay 5 seconds @21:25:16.46 PSV.dial :waste1 @21:25:16.90 PS.seek 0ml @21:25:33.03 PSV.dial PSR @21:25:33.47 PSR.select :air @21:25:33.58 PS.seek 2.5ml @21:25:59.76 PRV.dial CBV,avoiding: :ab2 @21:25:59.78
CBV.dial PRV @21:26:01.35 PSV.dial :mixloop @21:26:02.33 CTV.dial :air,avoiding: :intake @21:26:02.34 PTV.dial :PRVmixing @21:26:03.06 PS.seek 0ml @21:26:29.28 PSV.dial :air @21:26:29.71 PS.seek 2.5ml @21:26:55.84 PSV.dial :mixloop @21:26:56.30 PS.seek 0ml @21:27:22.56 PSV.dial :air @21:27:23.01 PS.seek 2.5ml @21:27:49.19 PSV.dial :mixloop @21:27:49.63 PS.seek 0ml @21:28:15.87 Garaging FlushPuck in Collection Clamp @21:28:15.94 Elbow.reconfigure :tight @21:28:15.98 Elbow.seek CC @21:28:16.09 Elbow.reconfigure :loose @21:28:16.18 Forearm.seek CC @21:28:17.13 Hand.close @21:28:17.90 CC.seek :open @21:28:22.21 Forearm.seek :retract @21:28:23.27 Elbow.reconfigure :tight @21:28:23.31 Elbow.seek :garage @21:28:26.34 Elbow.reconfigure :loose @21:28:26.43 Forearm.reconfigure :tight @21:28:26.47 Forearm.seek :garage @21:28:27.60 Hand.open @21:28:28.45 Forearm.reconfigure :loose @21:28:28.49 Forearm.seek :retract @21:28:29.50 delayUntil "21:32:38.76" @21:28:29.53 Whole Cell Archive sampling 100ml @21:28:29.54 Loading wcr puck from tube 4 into Collection Clamp @21:28:29.76 SC.reconfigure :tight @21:28:29.81 SC.seek 4 @21:28:29.90 SC.configure :limp @21:28:30.10 Elbow.reconfigure :tight @21:28:30.15 Elbow.seek 4 @21:28:32.05 Forearm.reconfigure :tight @21:28:32.10 Forearm.seek 4 + 700 counts @21:28:33.09 SE.reconfigure :pick @21:28:33.15 SE.seek :beamBreak @21:28:34.67 SE.configure :default @21:28:34.82 Elbow.seek 4 @21:28:34.99 Forearm.seek 4 + 300 counts @21:28:35.24 Forearm.reconfigure :loose @21:28:35.28 Hand.close @21:28:35.90 SE.seek :down @21:28:36.11 Forearm.seek :retract @21:28:37.13 Elbow.seek CC @21:28:37.20 SE.stop @21:28:38.72 Elbow.reconfigure :loose @21:28:38.81 Forearm.reconfigure :tight @21:28:38.86 Forearm.seek CC @21:28:40.55 CC.seek :closed @21:28:45.30 Collection Clamp onSpring/offSpring for 9/26 samples, on/off torque 1.67 @21:28:45.32 Hand.open @21:28:46.08 Forearm.reconfigure :loose @21:28:46.12 Forearm.seek :retract @21:28:47.10 collectSample 100ml w/bubble point of 35psi @21:28:47.11 Priming 7ml @21:28:47.12 Intake.select :close @21:28:47.13 Exhaust.select :close @21:28:47.14 SS.hold @21:28:47.33 Calibrating pressure sensors @21:28:47.34 CTV.dial :air @21:28:47.36 SSV.dial :spit,avoiding: :exhaust @21:28:47.38 SS.reconfigure :fast @21:28:47.42 SS.seek 5ml @21:28:50.23 SSV.dial CBV @21:28:50.25 CBV.dial SSV @21:28:51.55 delay 3.8 seconds @21:28:55.48 SSV.dial :spit @21:28:56.78 SS.seek 0ml @21:28:59.61 SSV.dial CBV @21:29:00.92 CTV.dial :intake @21:29:01.65 SSV.dial CBV @21:29:01.68 CBV.dial SSV @21:29:01.69 Allowing 40s for 7.0ml of remaining 7.0ml @21:29:01.70 Intake.select :open @21:29:03.86 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:29:03.88 SS.reconfigure :inhaling 2.14ml/s @21:29:03.92 SS.seek 7ml @21:29:10.63 Stroke completed -- waiting for pressure to equalize ... @21:29:10.64 SS.reconfigure :exhale @21:29:10.68 SS.hold @21:29:20.37 Intake.select :close @21:29:20.51 SSV.dial :exhaust @21:29:21.22 Exhaust.select :open @21:29:21.33 SS.seek 0ml @21:29:25.53 Exhaust.select :close @21:29:25.64 Sampling 100.0ml @21:29:25.65 Intake.select :close @21:29:25.66 Exhaust.select :close @21:29:25.67 SS.reconfigure :default @21:29:25.71 SS.hold @21:29:25.91 CTV.dial :intake @21:29:25.93 SSV.dial CBV @21:29:25.95 CBV.dial SSV @21:29:26.71 Allowing 130s for 25.0ml of remaining 100.0ml @21:29:26.72 Intake.select :open @21:29:28.88 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:29:28.90 SS.reconfigure :inhaling 2.14ml/s @21:29:28.94 SS.seek 25ml @21:29:33.76 Vacuum too high -- waiting up to 40 seconds @21:29:33.77 SS.reconfigure :exhale @21:29:33.82 SS.hold @21:29:35.76 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:29:35.78 SS.reconfigure :inhaling 1.93ml/s @21:29:35.82 SS.seek 25ml @21:29:40.45 Vacuum too high -- waiting up to 40 seconds @21:29:40.46 SS.reconfigure :exhale @21:29:40.50 SS.hold @21:29:43.72 Intake @14.3psia, vacuum from 8.8 to 11.8psi @21:29:43.74 SS.reconfigure :inhaling 1.74ml/s @21:29:43.79 SS.seek 25ml @21:29:49.92 Vacuum too high -- waiting up to 40 seconds @21:29:50.41 SS.reconfigure :exhale @21:29:50.45 SS.hold @21:29:53.85 Intake @14.3psia, vacuum from 8.8 to 11.8psi @21:29:53.87 SS.reconfigure :inhaling 1.57ml/s @21:29:53.92 SS.seek 25ml @21:29:57.63 Stroke completed -- waiting for pressure to equalize ... @21:29:57.64 SS.reconfigure :exhale @21:29:57.68 SS.hold @21:30:00.08 Intake.select :close @21:30:00.23 SSV.dial :exhaust @21:30:00.97 Exhaust.select :open @21:30:01.08 SS.seek 0ml @21:30:10.14 Exhaust.select :close @21:30:10.25 SSV.dial CBV @21:30:10.28 CBV.dial SSV @21:30:11.02 Allowing 130s for 25.0ml of remaining 75.0ml @21:30:11.03 Intake.select :open @21:30:13.19 Intake @16.0psia, vacuum from 10.0 to 13.0psi @21:30:13.21 SS.reconfigure :inhaling 1.57ml/s @21:30:13.25 SS.seek 25ml @21:30:18.51 Vacuum too high -- waiting up to 40 seconds @21:30:18.52 SS.reconfigure :exhale @21:30:18.56 SS.hold @21:30:19.33 Intake @13.6psia, vacuum from 8.1 to 11.1psi @21:30:19.35 SS.reconfigure :inhaling 1.41ml/s @21:30:19.39 SS.seek 25ml @21:30:29.04 Vacuum too high -- waiting up to 40 seconds @21:30:29.05 SS.reconfigure :exhale @21:30:29.10 SS.hold @21:30:32.16 Intake @14.6psia, vacuum from 9.1 to 12.1psi @21:30:32.17 SS.reconfigure :inhaling 1.27ml/s @21:30:32.22 SS.seek 25ml @21:30:39.28 Stroke completed -- waiting for pressure to equalize ... @21:30:39.29 SS.reconfigure :exhale @21:30:39.34 SS.hold @21:30:41.73 Intake.select :close @21:30:41.88 SSV.dial :exhaust @21:30:42.63 Exhaust.select :open @21:30:42.78 SS.seek 0ml @21:30:51.84 Exhaust.select :close @21:30:51.95 SSV.dial CBV @21:30:51.99 CBV.dial SSV @21:30:52.72 Allowing 130s for 25.0ml of remaining 50.0ml @21:30:52.73 Intake.select :open @21:30:54.89 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:30:54.91 SS.reconfigure :inhaling 1.27ml/s @21:30:54.95 SS.seek 25ml @21:31:04.48 Vacuum too high -- waiting up to 40 seconds @21:31:04.49 SS.reconfigure :exhale @21:31:04.53 SS.hold @21:31:05.52 Intake @13.6psia, vacuum from 8.1 to 11.1psi @21:31:05.54 SS.reconfigure :inhaling 1.14ml/s @21:31:05.59 SS.seek 25ml @21:31:20.28 Stroke completed -- waiting for pressure to equalize ... @21:31:20.29 SS.reconfigure :exhale @21:31:20.33 SS.hold @21:31:22.73 Intake.select :close @21:31:22.88 SSV.dial :exhaust @21:31:23.61 Exhaust.select :open @21:31:23.72 SS.seek 0ml @21:31:33.15 Exhaust.select :close @21:31:33.26 SSV.dial CBV @21:31:33.30 CBV.dial SSV @21:31:34.05 Allowing 130s for 25.0ml of remaining 25.0ml @21:31:34.06 Intake.select :open @21:31:36.22 Intake @15.9psia, vacuum from 10.0 to 13.0psi @21:31:36.24 SS.reconfigure :inhaling 1.14ml/s @21:31:36.28 SS.seek 25ml @21:31:58.54 Vacuum too high -- waiting up to 40 seconds @21:31:58.55 SS.reconfigure :exhale @21:31:58.59 SS.hold @21:31:59.89 Intake @13.8psia, vacuum from 8.3 to 11.3psi @21:31:59.91 SS.reconfigure :inhaling 1.03ml/s @21:31:59.95 SS.seek 25ml @21:32:03.39 Stroke completed -- waiting for pressure to equalize ... @21:32:03.40 SS.reconfigure :exhale @21:32:03.44 SS.hold @21:32:04.64 Intake.select :close @21:32:04.78 SSV.dial :exhaust @21:32:05.53 Exhaust.select :open @21:32:05.64 SS.seek 0ml @21:32:14.69 Exhaust.select :close @21:32:14.80 Sampled 100.0ml @21:32:14.82 Drying puck before fixing for 20:00 @21:32:14.83 CBV.dial :waste2b @21:32:15.54 delay 1:00 @21:32:38.77 recoverPRVreagent08 @21:32:38.78 PTV.dial :mixing @21:32:39.49 PBV.dial :waste1 @21:32:39.87 delay 30 seconds @21:33:09.88 PBV.dial between PSV and waste1 @21:33:10.24 PSV.dial :air @21:33:10.70 PS.reconfigure :fast @21:33:10.74 PS.seek 2.5ml @21:33:15.55 CSV.dial :air @21:33:15.98 CS.reconfigure :fast @21:33:16.02 CS.seek 10ml @21:33:33.96 PSV.dial :mixloop @21:33:34.41 delay 2 seconds @21:33:36.43 PSV.dial :air @21:33:36.88 delay 2 seconds @21:33:38.82 Pumping wcr puck from tube 4 up to 35psi... @21:33:38.83 CBV.dial SSV @21:33:38.90 PSV.dial :mixloop @21:33:39.35 delay 2 seconds @21:33:40.12 CBV.dial between CSV and waste2b @21:33:41.06 CSV.dial between air and CTV @21:33:41.39 CS.seek 7.346ml @21:33:41.43 PSV.dial :air @21:33:41.89 PS.seek 0ml @21:33:48.04 CTV.dial CSV,avoiding: :air @21:33:48.06 CSV.dial CTV @21:33:48.96 CS.reconfigure :pressurize @21:33:49.01 CS.seek 0ml @21:34:02.30 CBV.dial :waste2b @21:34:02.61 CS.reconfigure :default @21:34:02.66 CS.seek 0ml @21:34:04.71 washCycleDA #1 of 2 @21:34:04.72 PSR.select :air @21:34:04.73 PSV.dial PSR @21:34:06.04 Pulling 0.5ml of PSR.wash @21:34:06.05 PSR.select :wash @21:34:06.17 PS.reconfigure :default @21:34:06.21 PS.seek 0.5ml @21:34:09.06 delay 1:00 @21:34:12.08 delay 5 seconds @21:34:17.10 PSR.select :air @21:34:17.21 PS.seek 2ml @21:34:33.17 PTV.dial :puck @21:34:33.88 PSV.dial PTV @21:34:34.60 PBV.dial :waste1 @21:34:35.00 PS.seek 1.5ml @21:34:40.86 delay 3 seconds @21:34:43.88 PBV.dial between PSV and waste1 @21:34:44.25 PS.seek 0ml @21:35:00.33 delay 2:00 @21:35:09.09 CTV.dial :air,avoiding: :intake @21:35:10.35 Fix #1 of 2 (with RNAlater) for 10:00 @21:35:10.36 CSV.dial CSR @21:35:10.96 Pulling 2ml of CSR.RNAlater @21:35:10.97 CSR.select :RNAlater @21:35:11.08 CS.seek 2ml @21:35:16.97 delay 5 seconds @21:35:21.99 CSR.select :air @21:35:22.56 CS.reconfigure :fast @21:35:22.60 CS.seek 10ml @21:35:40.94 Pumping wcr puck from tube 4 up to 35psi... @21:35:40.96 CBV.dial SSV @21:35:42.24 CBV.dial between CSV and waste2b @21:35:43.17 CSV.dial between air and CTV @21:35:43.74 CS.seek 7.39ml @21:35:50.38 CTV.dial CSV,avoiding: :air @21:35:50.41 CSV.dial CTV @21:35:51.71 CS.reconfigure :pressurize @21:35:51.75 CS.seek 0ml @21:36:08.80 delay 10:00 @21:37:00.35 recoverPSRreagent08 @21:37:00.36 PTV.dial :puck @21:37:00.37 PBV.dial :waste1 @21:37:00.72 delay 30 seconds @21:37:30.74 PBV.dial between PSV and waste1 @21:37:31.09 PSR.select :air @21:37:31.11 PSV.dial PSR @21:37:31.83 PS.reconfigure :fast @21:37:31.88 PS.seek 2.5ml @21:37:54.54 PSV.dial PTV @21:37:55.26 delay 5 seconds @21:38:00.28 PSV.dial PSR @21:38:01.00 delay 5 seconds @21:38:06.02 PSV.dial PTV @21:38:06.73 delay 5 seconds @21:38:11.75 PSV.dial :air @21:38:12.47 PS.seek 0ml @21:38:35.08 washCycleDA #2 of 2 @21:38:35.09 PSR.select :air @21:38:35.10 PSV.dial PSR @21:38:36.42 Pulling 0.5ml of PSR.wash @21:38:36.43 PSR.select :wash @21:38:36.55 PS.reconfigure :default @21:38:36.59 PS.seek 0.5ml @21:38:42.42 delay 5 seconds @21:38:47.44 PSR.select :air @21:38:47.55 PS.seek 2ml @21:39:03.63 PTV.dial :puck @21:39:03.65 PSV.dial PTV @21:39:04.36 PBV.dial :waste1 @21:39:04.77 PS.seek 1.5ml @21:39:10.70 delay 3 seconds @21:39:13.72 PBV.dial between PSV and waste1 @21:39:14.08 PS.seek 0ml @21:39:30.07 delay 2:00 @21:41:30.10 recoverPSRreagent08 @21:41:30.11 PTV.dial :puck @21:41:30.12 PBV.dial :waste1 @21:41:30.48 delay 30 seconds @21:42:00.50 PBV.dial between PSV and waste1 @21:42:00.85 PSR.select :air @21:42:00.87 PSV.dial PSR @21:42:01.59 PS.reconfigure :fast @21:42:01.64 PS.seek 2.5ml @21:42:24.60 PSV.dial PTV @21:42:25.32 delay 5 seconds @21:42:30.34 PSV.dial PSR @21:42:31.05 delay 5 seconds @21:42:36.07 PSV.dial PTV @21:42:36.79 delay 5 seconds @21:42:41.81 PSV.dial :air @21:42:42.53 PS.seek 0ml @21:43:05.01 getPMLReagent08 @21:43:05.02 PRV.dial :air @21:43:05.46 delay 5 seconds @21:43:10.48 PTV.dial :PRVmixing @21:43:11.74 delay 5 seconds @21:43:16.76 PRV.dial :ab2 @21:43:17.21 PTV.dial :PRVmixing @21:43:17.22 PSV.dial :mixloop @21:43:17.67 PS.reconfigure :default @21:43:17.71 PS.seek 0.3ml @21:43:21.76 delay 2 seconds @21:43:23.78 PRV.dial :air @21:43:24.30 PS.seek 0.55ml @21:43:27.72 addPMLReagent08 @21:43:27.73 PSV.dial :air @21:43:28.18 PS.seek 2ml @21:43:43.69 PSV.dial :PML @21:43:44.13 PTV.dial :mixing @21:43:44.88 PBV.dial :waste1 @21:43:45.29 PS.seek 1.85ml @21:43:47.72 PBV.dial between PSV and waste1 @21:43:48.09 PS.seek 0ml @21:44:07.63 delay 2 seconds @21:44:09.64 delay 8:00 @21:46:08.83 CBV.dial :waste2b @21:46:09.14 CS.reconfigure :default @21:46:09.19 CS.seek 0ml @21:46:11.92 delay 1:00 @21:47:11.95 CTV.dial :air,avoiding: :intake @21:47:13.22 Fix #2 of 2 (with RNAlater) for 10:00 @21:47:13.23 CSV.dial CSR @21:47:14.16 Pulling 2ml of CSR.RNAlater @21:47:14.17 CSR.select :RNAlater @21:47:14.29 CS.seek 2ml @21:47:20.20 delay 5 seconds @21:47:25.22 CSR.select :air @21:47:25.33 CS.reconfigure :fast @21:47:25.38 CS.seek 10ml @21:47:43.57 Pumping wcr puck from tube 4 up to 35psi... @21:47:43.59 CBV.dial SSV @21:47:44.86 CBV.dial between CSV and waste2b @21:47:45.77 CSV.dial between air and CTV @21:47:46.36 CS.seek 7.288ml @21:47:53.14 CTV.dial CSV,avoiding: :air @21:47:53.17 CSV.dial CTV @21:47:54.58 CS.reconfigure :pressurize @21:47:54.63 CS.seek 0ml @21:48:11.50 delay 10:00 @21:52:09.67 recoverPRVreagent08 @21:52:09.68 PTV.dial :mixing @21:52:09.70 PBV.dial :waste1 @21:52:10.06 delay 30 seconds @21:52:40.08 PBV.dial between PSV and waste1 @21:52:40.44 PSV.dial :air @21:52:40.90 PS.reconfigure :fast @21:52:40.95 PS.seek 2.5ml @21:53:04.02 PSV.dial :mixloop @21:53:04.47 delay 2 seconds @21:53:06.49 PSV.dial :air @21:53:06.94 delay 2 seconds @21:53:08.96 PSV.dial :mixloop @21:53:09.41 delay 2 seconds @21:53:11.43 PSV.dial :air @21:53:11.89 PS.seek 0ml @21:53:34.52 washCycleDA #1 of 3 @21:53:34.53 PSR.select :air @21:53:34.55 PSV.dial PSR @21:53:35.87 Pulling 0.5ml of PSR.wash @21:53:35.88 PSR.select :wash @21:53:35.99 PS.reconfigure :default @21:53:36.04 PS.seek 0.5ml @21:53:41.96 delay 5 seconds @21:53:46.98 PSR.select :air @21:53:47.09 PS.seek 2ml @21:54:03.08 PTV.dial :puck @21:54:03.78 PSV.dial PTV @21:54:04.50 PBV.dial :waste1 @21:54:04.93 PS.seek 1.5ml @21:54:10.83 delay 3 seconds @21:54:13.85 PBV.dial between PSV and waste1 @21:54:14.21 PS.seek 0ml @21:54:30.17 delay 2:00 @21:56:30.20 recoverPSRreagent08 PTV.dial :puck @21:56:30.21 PBV.dial :waste1 @21:56:30.56 delay 30 seconds @21:57:00.59 PBV.dial between PSV and waste1 @21:57:00.94 PSR.select :air @21:57:00.95 PSV.dial PSR @21:57:01.69 PS.reconfigure :fast @21:57:01.74 PS.seek 2.5ml @21:57:24.61 PSV.dial PTV @21:57:25.33 delay 5 seconds @21:57:30.35 PSV.dial PSR @21:57:31.08 delay 5 seconds @21:57:36.09 PSV.dial PTV @21:57:36.81 delay 5 seconds @21:57:41.83 PSV.dial :air @21:57:42.55 PS.seek 0ml @21:58:05.36 washCycleDA #2 of 3 @21:58:05.37 PSR.select :air @21:58:05.38 PSV.dial PSR @21:58:06.69 Pulling 0.5ml of PSR.wash @21:58:06.70 PSR.select :wash @21:58:06.81 PS.reconfigure :default @21:58:06.85 PS.seek 0.5ml @21:58:11.52 CBV.dial :waste2b @21:58:11.84 CS.reconfigure :default @21:58:11.89 CS.seek 0ml @21:58:12.76 delay 5 seconds @21:58:14.78 delay 1:00 @21:58:17.78 PSR.select :air @21:58:17.90 PS.seek 2ml @21:58:33.85 PTV.dial :puck @21:58:33.87 PSV.dial PTV @21:58:34.57 PBV.dial :waste1 @21:58:34.98 PS.seek 1.5ml @21:58:40.85 delay 3 seconds @21:58:43.87 PBV.dial between PSV and waste1 @21:58:44.24 PS.seek 0ml @21:59:00.26 delay 2:00 @21:59:14.80 CTV.dial :air,avoiding: :intake @22:01:00.28 recoverPSRreagent08 @22:01:00.30 PTV.dial :puck @22:01:00.31 PBV.dial :waste1 @22:01:00.66 delay 30 seconds @22:01:30.69 PBV.dial between PSV and waste1 @22:01:31.04 PSR.select :air @22:01:31.54 PSV.dial PSR @22:01:32.39 PS.reconfigure :fast @22:01:32.43 PS.seek 2.5ml @22:01:55.38 PSV.dial PTV @22:01:56.09 delay 5 seconds @22:02:01.11 PSV.dial PSR @22:02:01.82 delay 5 seconds @22:02:06.84 PSV.dial PTV @22:02:07.55 delay 5 seconds @22:02:12.57 PSV.dial :air @22:02:13.29 PS.seek 0ml @22:02:36.11 washCycleDA #3 of 3 @22:02:36.12 PSR.select :air @22:02:36.13 PSV.dial PSR @22:02:37.45 Pulling 0.5ml of PSR.wash @22:02:37.46 PSR.select :wash @22:02:37.57 PS.reconfigure :default @22:02:37.61 PS.seek 0.5ml @22:02:43.57 delay 5 seconds @22:02:48.59 PSR.select :air @22:02:48.70 PS.seek 2ml @22:03:04.75 PTV.dial :puck @22:03:04.77 PSV.dial PTV @22:03:05.47 PBV.dial :waste1 @22:03:05.89 PS.seek 1.5ml @22:03:11.85 delay 3 seconds @22:03:14.86 PBV.dial between PSV and waste1 @22:03:15.23 PS.seek 0ml @22:03:31.32 delay 2:00 @22:05:31.34 recoverPSRreagent08 @22:05:31.35 PTV.dial :puck @22:05:31.36 PBV.dial :waste1 @22:05:31.72 delay 30 seconds @22:06:01.74 PBV.dial between PSV and waste1 @22:06:02.09 PSR.select :air @22:06:02.11 PSV.dial PSR @22:06:02.96 PS.reconfigure :fast @22:06:03.00 PS.seek 2.5ml @22:06:25.75 PSV.dial PTV @22:06:26.45 delay 5 seconds @22:06:31.47 PSV.dial PSR @22:06:32.19 delay 5 seconds @22:06:37.21 PSV.dial PTV @22:06:37.92 delay 5 seconds @22:06:42.94 PSV.dial :air @22:06:43.66 PS.seek 0ml @22:07:06.56 Gate.power :camera,:ON @22:07:06.57 Mixing DA Substrates @22:07:06.58 PSR.select :air @22:07:06.60 PSV.dial PSR @22:07:08.14 Pulling 0.25ml of PSR.sub1 @22:07:08.17 PSR.select :sub1 @22:07:08.49 PS.reconfigure :default @22:07:08.64 PS.seek 0.25ml @22:07:12.12 delay 5 seconds @22:07:17.19 Pulling 0.25ml of PSR.sub2 @22:07:17.20 PSR.select :sub2 @22:07:17.32 PS.seek 0.5ml @22:07:20.62 delay 5 seconds @22:07:25.64 PSR.select :air @22:07:25.75 PS.seek 2ml @22:07:41.84 PTV.dial :puck @22:07:41.85 PSV.dial PTV @22:07:42.56 PBV.dial :waste1 @22:07:42.98 PS.seek 1.5ml @22:07:48.81 delay 3 seconds @22:07:51.83 PBV.dial between PSV and waste1 @22:07:52.19 PS.seek 0ml @22:08:08.21 delay 10 seconds @22:08:18.24 recoverPSRreagent08 @22:08:18.25 PTV.dial :puck @22:08:18.26 PBV.dial :waste1 @22:08:18.62 delay 30 seconds @22:08:48.65 PBV.dial between PSV and waste1 @22:08:48.99 PSR.select :air @22:08:49.01 PSV.dial PSR @22:08:49.75 PS.reconfigure :fast @22:08:49.79 PS.seek 2.5ml @22:09:12.55 PSV.dial PTV @22:09:13.29 delay 5 seconds @22:09:18.31 PSV.dial PSR @22:09:19.04 delay 5 seconds @22:09:24.06 PSV.dial PTV @22:09:24.79 delay 5 seconds @22:09:29.81 PSV.dial :air @22:09:30.55 PS.seek 0ml @22:09:53.65 Additional air clear @22:09:53.66 PSR.select :air @22:09:53.67 PSV.dial PSR @22:09:55.00 Pulling 2ml of PSR.air @22:09:55.01 PSR.select :air @22:09:55.03 PS.reconfigure :default @22:09:55.07 PS.seek 2ml @22:10:16.15 delay 5 seconds @22:10:21.17 PTV.dial :puck @22:10:21.18 PSV.dial PTV @22:10:21.90 PBV.dial :waste1 @22:10:22.31 PS.seek 1.5ml @22:10:28.15 delay 3 seconds @22:10:31.16 PBV.dial between PSV and waste1 @22:10:31.54 delay 1 second @22:10:32.56 PSV.dial :waste1 @22:10:33.56 PS.seek 0ml @22:10:49.85 Processing Puck Heater at 28.2C before imaging @22:10:49.86 PH.coast @22:10:49.99 Imaging da puck in Processing Clamp while storing it to tube 3 @22:10:50.14 SC.reconfigure :tight @22:10:50.18 SC.seek 3 @22:10:53.78 SC.configure :limp @22:10:53.97 Elbow.reconfigure :tight @22:10:54.51 Elbow.seek 3 @22:10:56.32 Forearm.reconfigure :tight @22:10:56.38 Forearm.seek 3 + 700 counts @22:10:57.39 SE.reconfigure :pick @22:10:57.44 SE.seek :beamBreak @22:11:00.04 SE.configure :default @22:11:00.19 Elbow.reconfigure :limp @22:11:00.23 Forearm.reconfigure :loose @22:11:00.28 Forearm.seek 3 @22:11:00.70 Elbow.hold @22:11:00.76 Elbow.reconfigure :tight @22:11:00.85 SE.seek 7.23 Pucks below Top Plate @22:11:01.29 Forearm.force -20 @22:11:01.33 Forearm.seek 3 + 319 counts @22:11:01.65 Forearm.reconfigure :tight @22:11:01.70 Forearm.seek 3 + 700 counts @22:11:01.91 SE.seek 8.23 Pucks below Top Plate @22:11:02.49 Forearm.reconfigure :loose @22:11:02.53 Forearm.seek :retract @22:11:03.54 Elbow.seek PC @22:11:06.33 Elbow.reconfigure :loose @22:11:06.42 Forearm.seek PC @22:11:07.49 Hand.close @22:11:08.18 PC.seek :open @22:11:12.22 Forearm.seek :retract @22:11:13.29 Elbow.reconfigure :tight @22:11:13.34 Elbow.seek 3 @22:11:16.01 Forearm.reconfigure :tight @22:11:16.06 Forearm.seek 3 + 700 counts @22:11:17.18 Hand.open @22:11:17.99 SE.seek 7.88 Pucks below Top Plate @22:11:18.27 SE.reconfigure :seal @22:11:18.31 SE.seek 6.77 Pucks below Top Plate @22:11:18.86 Lights, camera, ACTION! @22:11:18.89 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da23apr1522h100ml.tif @22:11:38.35 Waiting >20 seconds for da to relinquish FlushPuck @22:11:59.47 SX MX5/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 36.81 seconds /var/log/esp/da23apr1522h100ml.tif: TIFF Upload Complete @22:11:59.50 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da23apr1522h100ml15s.tif @22:12:15.23 SX MX5/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da23apr1522h100ml15s.tif: TIFF Upload Complete @22:12:15.25 PuckCamera: snap -bin=2,2 25.0 /var/log/esp/midres/da23apr1522h100ml25s.tif @22:12:41.05 SX MX5/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25 seconds /var/log/esp/midres/da23apr1522h100ml25s.tif: TIFF Upload Complete @22:12:41.06 Gate.power :camera,:OFF @22:12:41.08 DAfans.email "36.81 sec auto-exposure",Subject: "da23apr1522h100ml images!" @22:12:41.39 Elbow.seek 3 @22:12:41.76 Forearm.seek 3 + 700 counts @22:12:41.85 SE.reconfigure :gently @22:12:41.89 SE.seek 7.48 Pucks below Top Plate @22:12:42.55 Elbow.reconfigure :limp @22:12:42.61 Forearm.reconfigure :loose @22:12:42.65 Forearm.seek 3 @22:12:43.06 Elbow.hold @22:12:43.12 Elbow.reconfigure :tight @22:12:43.17 SE.reconfigure :default @22:12:43.22 SE.seek 7.67 Pucks below Top Plate @22:12:43.61 Forearm.force -20 @22:12:43.64 Forearm.seek 3 + 315 counts @22:12:43.95 Forearm.reconfigure :tight @22:12:44.00 Forearm.seek 3 + 700 counts @22:12:44.43 SE.seek :down @22:12:45.67 Forearm.reconfigure :loose @22:12:45.76 Forearm.seek :retract @22:12:46.69 SE.stop @22:12:48.15 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:12:48.66 Elbow.seek FlushPuck::Garage @22:12:50.68 Elbow.reconfigure :loose @22:12:50.87 Forearm.seek :garage @22:12:52.04 Hand.close @22:12:52.93 Forearm.seek :retract @22:12:54.09 Elbow.reconfigure :tight @22:12:54.17 Elbow.seek PC @22:12:55.58 Elbow.reconfigure :loose @22:12:55.76 Forearm.reconfigure :tight @22:12:55.84 Forearm.seek PC @22:12:57.63 PC.seek :closed @22:13:01.68 Processing Clamp onSpring/offSpring for 9/24 samples, on/off torque 1.38 @22:13:01.83 Hand.open @22:13:02.69 Forearm.reconfigure :loose @22:13:02.78 Forearm.seek :retract @22:13:03.87 PSR.select :air @22:13:03.93 Storing wcr puck in Collection Clamp to tube 3 @22:13:03.99 PSV.dial PSR @22:13:04.43 SC.reconfigure :tight @22:13:04.52 SC.seek 3 @22:13:04.78 SC.configure :limp @22:13:04.83 Pulling 0.5ml of PSR.flush @22:13:04.87 PSR.select :flush @22:13:05.38 PS.seek 0.5ml @22:13:05.52 Elbow.reconfigure :tight @22:13:05.60 Elbow.seek 3 @22:13:08.46 Forearm.reconfigure :tight @22:13:08.56 Forearm.seek 3 + 700 counts @22:13:09.70 SE.reconfigure :pick @22:13:09.89 SE.seek :beamBreak @22:13:11.27 delay 5 seconds @22:13:12.54 SE.configure :default @22:13:12.84 Elbow.reconfigure :limp @22:13:12.94 Forearm.reconfigure :loose @22:13:13.10 Forearm.seek 3 @22:13:13.57 Elbow.hold @22:13:13.70 Elbow.reconfigure :tight @22:13:13.89 SE.seek 8.23 Pucks below Top Plate @22:13:14.52 Forearm.force -20 @22:13:14.60 Forearm.seek 3 + 298 counts @22:13:15.08 Forearm.reconfigure :tight @22:13:15.17 Forearm.seek 3 + 700 counts @22:13:15.44 SE.seek 9.23 Pucks below Top Plate @22:13:16.19 Forearm.reconfigure :loose @22:13:16.27 Forearm.seek :retract @22:13:16.37 PSR.select :air @22:13:16.55 PS.seek 2.5ml @22:13:17.23 Elbow.seek CC @22:13:17.34 upsync @22:13:18.75 Elbow.reconfigure :loose @22:13:18.84 Forearm.seek CC @22:13:19.85 Hand.close @22:13:20.56 CC.seek :open @22:13:25.00 Forearm.seek :retract @22:13:26.10 Elbow.reconfigure :tight @22:13:26.15 Elbow.seek 3 @22:13:27.92 Elbow.reconfigure :limp @22:13:28.45 Forearm.seek 3 @22:13:29.58 Elbow.hold @22:13:29.65 Elbow.reconfigure :tight @22:13:29.70 Hand.open @22:13:30.54 Forearm.force -20 @22:13:30.59 Forearm.seek 3 + 312 counts @22:13:30.98 Forearm.reconfigure :tight @22:13:31.03 Forearm.seek 3 + 700 counts @22:13:31.42 SE.seek :down @22:13:32.54 Forearm.reconfigure :loose @22:13:32.59 Forearm.seek :retract @22:13:33.57 SE.stop @22:13:33.62
Starting finalPurge @22:13:37.64 delay 1 second @22:13:38.66 PRV.dial :waste2,avoiding: :ab2 @22:13:40.45 PTV.dial :PRVmixing @22:13:41.70 PSV.dial :mixloop @22:13:42.71 PS.seek :empty @22:13:52.65
Waiting >20 seconds for psFlush to relinquish FlushPuck @22:14:08.92 PSR.select :air @22:14:08.94 PSV.dial PSR @22:14:09.99 Pulling 0.5ml of PSR.flush @22:14:10.00 PSR.select :flush @22:14:10.12 PS.seek 0.5ml @22:14:15.96 delay 5 seconds @22:14:20.97 PSR.select :air @22:14:21.09 PS.seek 2.5ml @22:14:42.17 delay 1 second @22:14:43.19 PBV.dial :waste1 @22:14:43.55 PTV.dial :mixing @22:14:44.27 PSV.dial :mixloop @22:14:45.27 PS.seek :empty @22:15:11.48 PSV.dial PSR @22:15:12.48 PSR.select :flush @22:15:12.60 PS.seek 1ml @22:15:23.54 PSR.select :air @22:15:23.66 PS.seek 2.5ml @22:15:39.67 PSV.dial PTV @22:15:40.39 PTV.dial :puck @22:15:41.09 PBV.dial :waste1 @22:15:41.11 PS.seek 0ml @22:16:07.32 PSV.dial PSR @22:16:08.04 PSR.select :flush @22:16:08.16 PS.seek 0.5ml @22:16:14.13 PSR.select :air @22:16:14.25 PS.seek 2.5ml @22:16:35.42 PSV.dial PTV @22:16:36.14 PBV.dial PSV @22:16:36.69 PS.seek 0ml @22:17:02.82 PSV.dial PBV @22:17:03.28 PS.seek 2.5ml @22:17:29.42 PSV.dial :waste1 @22:17:30.12 PS.seek 0ml @22:17:56.29 PSV.dial PSR @22:17:56.85 PSR.select :air @22:17:56.88 PS.seek 2.5ml @22:18:23.23 PSV.dial PTV @22:18:23.96 PBV.dial PSV @22:18:23.98 PS.seek 0ml @22:18:50.23 PSV.dial PBV @22:18:50.69 PS.seek 2.5ml @22:19:17.10 PSV.dial :waste1 @22:19:17.81 PS.seek 0ml @22:19:43.98 PSV.dial PSR @22:19:44.54 PSR.select :flush @22:19:44.65 PS.seek 1ml @22:19:55.66 PSR.select :air @22:19:55.77 PS.seek 2.5ml @22:20:11.88 PSV.dial PTV @22:20:12.61 PTV.dial :puck @22:20:12.63 PBV.dial :waste1 @22:20:13.17 PS.seek 0ml @22:20:39.38 PSV.dial PSR @22:20:40.10 PSR.select :flush @22:20:40.21 PS.seek 0.5ml @22:20:46.19 PSR.select :air @22:20:46.30 PS.seek 2.5ml @22:21:07.47 PSV.dial PTV @22:21:08.19 PBV.dial PSV @22:21:08.74 PS.seek 0ml @22:21:35.35 PSV.dial PBV @22:21:35.81 PS.seek 2.5ml @22:22:02.06 PSV.dial :waste1 @22:22:02.77 PS.seek 0ml @22:22:28.94 PSV.dial PSR @22:22:29.50 PSR.select :air @22:22:29.53 PS.seek 2.5ml @22:22:55.69 PSV.dial PTV @22:22:56.42 PBV.dial PSV @22:22:56.44 PS.seek 0ml @22:23:22.62 PSV.dial PBV @22:23:23.08 PS.seek 2.5ml @22:23:49.31 PSV.dial :waste1 @22:23:50.02 PS.seek 0ml @22:24:16.19
Loading FlushPuck into Collection Clamp from Processing Clamp @22:24:16.36 Elbow.seek PC @22:24:18.96 Elbow.reconfigure :loose @22:24:19.05 Forearm.seek PC @22:24:20.15 Hand.close @22:24:20.78 PC.seek :open @22:24:24.73 Forearm.seek :retract @22:24:25.75 Elbow.reconfigure :tight @22:24:25.79 Elbow.seek CC @22:24:29.25 Elbow.reconfigure :loose @22:24:29.34 Forearm.reconfigure :tight @22:24:29.38 Forearm.seek CC @22:24:30.61 CC.seek :closed @22:24:34.84 Collection Clamp onSpring/offSpring for 9/25 samples, on/off torque 1.79 @22:24:34.91 Hand.open @22:24:35.72 Forearm.reconfigure :loose @22:24:35.76 Forearm.seek :retract @22:24:36.86 Flushing the Collection Valve::Manifold @22:24:36.97 CSV.dial CSR @22:24:37.44 Pulling 5ml of CSR.flush @22:24:37.45 CSR.select :flush @22:24:37.56 CS.seek 5ml @22:24:51.08 delay 5 seconds @22:24:56.09 CSR.select :air @22:24:56.24 CS.reconfigure :fast @22:24:56.29 CS.seek 7.5ml @22:25:02.67 CSV.dial :waste @22:25:03.91 CS.reconfigure :default @22:25:03.95 CS.seek 0ml @22:25:23.73 Flushing lower manifold's waste1 port @22:25:23.85 CSV.dial :flush @22:25:24.28 CS.reconfigure :fast @22:25:24.33 CS.seek 5ml @22:25:36.17 delay 5 seconds @22:25:41.18 CSV.dial :air @22:25:41.68 CS.seek 7ml @22:25:47.02 CSV.dial CTV @22:25:47.04
CTV.dial CSV @22:25:48.35 CBV.dial :waste1 @22:25:49.11 CS.seek 0ml @22:26:05.23 Flushing lower manifold's waste2 port @22:26:05.34 CSV.dial :flush @22:26:06.07 CS.seek 5ml @22:26:17.82 delay 5 seconds @22:26:22.84 CSV.dial :air @22:26:23.34 CS.seek 7ml @22:26:28.57 CSV.dial CTV @22:26:28.62
CTV.dial CSV @22:26:29.03 CBV.dial :waste2 @22:26:30.05 CS.seek 0ml @22:26:46.04 Flushing lower manifold's waste2b port @22:26:46.16 CSV.dial :flush @22:26:46.87 CS.seek 5ml @22:26:58.63 delay 5 seconds @22:27:03.65 CSV.dial :air @22:27:04.18 CS.seek 7ml @22:27:09.38 CSV.dial CTV @22:27:09.41
CTV.dial CSV @22:27:09.84 CBV.dial :waste2b @22:27:10.84 CS.seek 0ml @22:27:26.79 Disinfecting external Intake with kill @22:27:27.37 CSV.dial CSR @22:27:27.86 Pulling 8ml of CSR.kill @22:27:27.87 CSR.select :kill @22:27:27.98 CS.seek 8ml @22:27:46.20 delay 5 seconds @22:27:51.22 CSR.select :air @22:27:51.33 CS.seek 10ml @22:27:56.61 CSV.dial CBV @22:27:56.64
CBV.dial CSV @22:27:57.66 CTV.dial :intake @22:27:58.38 Intake.select :open @22:27:58.49 CS.seek 2ml @22:28:16.66 Intake.select :close @22:28:16.76 CTV.dial :air @22:28:17.48 CS.reconfigure :default @22:28:17.52 CS.seek 0ml @22:28:23.47 CSV.dial CSR @22:28:24.51 Pulling 5ml of CSR.flush @22:28:24.52 CSR.select :flush @22:28:24.63 CS.seek 5ml @22:28:38.07 delay 5 seconds @22:28:43.09 CSR.select :air @22:28:43.20 CS.seek 10ml @22:28:56.63 CSV.dial CBV @22:28:57.75 CS.seek 0ml @22:29:23.76 Garaging FlushPuck in Collection Clamp @22:29:23.82 Elbow.reconfigure :tight @22:29:23.87 Elbow.seek CC @22:29:23.98 Elbow.reconfigure :loose @22:29:24.07 Forearm.seek CC @22:29:25.05 Hand.close @22:29:25.77 CC.seek :open @22:29:30.15 Forearm.seek :retract @22:29:31.19 Elbow.reconfigure :tight @22:29:31.24 Elbow.seek :garage @22:29:34.11 Elbow.reconfigure :loose @22:29:34.20 Forearm.reconfigure :tight @22:29:34.24 Forearm.seek :garage @22:29:35.36 Hand.open @22:29:36.20 Forearm.reconfigure :loose @22:29:36.25 Forearm.seek :retract @22:29:37.30 Completed [initialPurge; da [100, 100] #da and wsr are run simultanelously] begun 20:38:17 @22:29:37.35 Asleep after completing DA_WCR_041523 assays @22:29:37.37 GoodNews.email "{1 => 33,\n 2 => 33,\n 3 => 10,\n 4 => 23,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@22:27:20, 23.9C, 60% humidity, 15.9psia, 13.885V, 0.373A, 0.373A avg, 5.18W\nSampled 100.0ml",Subject: "Asleep after completing DA_WCR_041523 assays" @22:29:41.33 upsync