@15:42:50.46PDT03-Sep-25
Environmental Sample Processor built 28-Aug-25 (08574b7e) @15:42:54.17 >BREAK< ESP3 Gateway boot v1.42 for core bus @15:42:54.20 >BREAK< ESP3 Gateway boot v1.42 for cartridge bus @15:42:57.86 Configuration for marin by nadia@mbari.org 8/18/2025 /home/esp/esp2/type/portable/marin/configure.rb @15:42:58.39 Sea pressure (Abs) tare is 5% of full scale @15:43:00.00 Polling Can every 2:00 @15:43:00.06 Waiting for espclients... @15:43:01.48 Session Begun @15:43:07.86 -> ESP.ready! @15:43:07.93 Gate.power :main,:ON,limit to 5000mA @15:43:07.95 Gate.power :analytic1,:ON,limit to 4000mA @15:43:07.99 Gate.power :analytic2,:ON,limit to 4000mA @15:43:08.02 Gate.power :analytic3,:ON,limit to 4000mA @15:43:08.10 Powering core bus @15:43:10.86 Plunge.configure :default @15:43:10.96 SamplePump.configure :default @15:43:11.07 Abs.configure :default @15:43:11.17 Toroid.configure :default @15:43:11.24 PinPort.configure :default @15:43:11.35 Archive.configure :default @15:43:11.45 Air.configure :default @15:43:11.52 Lysis1.configure :default @15:43:11.61 Lysis2.configure :default @15:43:11.69 All elf controllers running firmware version 4.93 @15:43:11.73 Gate.power :motor,:ON,limit to 5000mA @15:43:12.16 Plunge.home.jog 0 @15:43:12.96 Lysis2.home.jog 0 @15:43:13.03 Air.home.jog 0 @15:43:13.07 PinPort.home.jog 0 @15:43:13.11 Archive.home.jog 0 @15:43:13.14 Toroid.home.jog 0 @15:43:13.18 Lysis1.home.jog 0 @15:43:13.91 Plunge.in :homing @15:43:14.30 Plunge.jog 551 @15:43:14.38 Lysis1.in :homing @15:43:14.45 Archive.in :homing @15:43:14.50 Toroid.in :homing @15:43:14.55 Air.in :homing @15:43:14.59 PinPort.in :homing @15:43:14.64 Lysis2.in :homing @15:43:14.98 Lysis1.jog 1662 @15:43:15.03 Archive.jog 1623 @15:43:15.06 Toroid.jog 826 @15:43:15.15 Air.jog 1567 @15:43:15.16 PinPort.jog -716 @15:43:15.21 Lysis2.jog 1617 @15:43:15.60 Plunge.setRawPosition! 50 @15:43:15.67 Plunge.seek :home @15:43:15.97 Plunge.in :default @15:43:16.48 PinPort.setRawPosition! -50 @15:43:16.56 PinPort.seek :home @15:43:16.70 Lysis1.setRawPosition! 50 @15:43:16.75 Air.setRawPosition! 50 @15:43:16.78 Archive.setRawPosition! 50 @15:43:16.95 Lysis1.seek :home @15:43:17.00 Air.seek :home @15:43:17.05 Archive.seek :home @15:43:17.33 Lysis2.setRawPosition! 50 @15:43:17.36 PinPort.in :default @15:43:17.52 Lysis2.seek :home @15:43:17.65 Air.in :default @15:43:17.68 Archive.in :default @15:43:17.73 Lysis1.in :default @15:43:17.96 Lysis2.in :default @15:43:18.15 Toroid.setRawPosition! 0 @15:43:18.22 Toroid.seek :home @15:43:18.33 Toroid.in :default @15:43:18.48 Toroid.seek :clear @15:43:18.66 PinPort.in :unlocking @15:43:18.74 PinPort.seek :clear @15:43:20.78 Air.seek :clear @15:43:21.45 Lysis1.seek :clear @15:43:22.37 Lysis2.seek :clear @15:43:23.20 Archive.seek :clear Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Abs = Absolute Pressure (Leak) Sensor @14psia Toroid = Toroid Valve (TV) clear PinPort = Handoff Pin Port (PP) clear Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear @15:43:30.69 -> cleanPortable @15:43:31.16 Plunge.in :unlocking @15:43:31.24 Plunge.seek :clear @15:43:31.92 Waiting for nadia to answer "LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue" @15:43:37.07 Answered LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue"" @15:43:37.56 Lysis1.in :fast @15:43:37.74 Lysis1.seek :locked @15:43:40.30 Plunge.in :default @15:43:40.40 Plunge.seek :locked @15:43:40.62 Air.in :fast @15:43:40.70 Air.seek :locked @15:43:41.70 Powering cartridge bus @15:43:44.37 Gate.power :cartridge,:OFF @15:43:45.03 Mounted archive Cartridge @15:43:45.09 Toroid.seek :bypassed @15:43:47.32 Priming sample loop w/10ml, bypass w/10ml @15:43:47.34 SamplePump.setPosition! 0ml @15:43:47.44 Bypass.open @15:43:49.08 Intake.open @15:43:50.09 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:43:52.71 Vacuum tare is 52% of full scale at 21C (drifted 0%) @15:43:52.89 Abs.in :default @15:43:52.97 Abs.hold @15:43:53.23 SamplePump.in :start @15:43:53.31 SamplePump.seek 10ml,1:10 @15:43:53.92 SamplePump.configure :prime @15:44:05.58 Abs.stop @15:44:05.66 SamplePump.setPosition! 0ml @15:44:05.74 Exhaust.open @15:44:07.38 Bypass.close @15:44:09.10 Abs.in :default @15:44:09.18 Abs.hold @15:44:09.51 SamplePump.in :start @15:44:09.59 SamplePump.seek 10ml,1:10 @15:44:10.20 SamplePump.configure :prime @15:44:21.86 Abs.stop @15:44:21.95 Waiting for nadia to answer "INTAKE line plumbed to 10% Bleach, push return to continue" @15:44:47.07 Answered INTAKE line plumbed to 10% Bleach, push return to continue"" @15:44:47.09 Toroid.seek :bypassed @15:44:47.23 Priming sample loop w/10ml, bypass w/10ml @15:44:47.25 SamplePump.setPosition! 0ml @15:44:47.34 Exhaust.close @15:44:48.20 Bypass.open @15:44:50.01 Abs.in :default @15:44:50.09 Abs.hold @15:44:50.34 SamplePump.in :start @15:44:50.46 SamplePump.seek 10ml,1:10 @15:44:51.03 SamplePump.configure :prime @15:45:02.75 Abs.stop @15:45:03.31 SamplePump.setPosition! 0ml @15:45:03.40 Exhaust.open @15:45:04.97 Bypass.close @15:45:06.69 Abs.in :default @15:45:06.76 Abs.hold @15:45:07.09 SamplePump.in :start @15:45:07.17 SamplePump.seek 10ml,1:10 @15:45:07.78 SamplePump.configure :prime @15:45:19.43 Abs.stop @15:45:19.52 Toroid.seek :cartridge @15:45:23.89 Sampling 25.0ml @15:45:23.91 SamplePump.setPosition! 0ml @15:45:24.18 Abs.in :default @15:45:24.26 Abs.hold @15:45:24.62 SamplePump.in :start @15:45:24.72 SamplePump.seek 25ml,2:10 @15:45:25.31 SamplePump.configure :sample @15:45:54.75 Abs.stop @15:45:54.83 Intake.close @15:45:54.91 Exhaust.close @15:45:55.04 Toroid.seek :bypassed @15:45:59.40 Sampled 25.0ml @15:45:59.42 delay 10:00 @15:55:59.48 Waiting for nadia to answer "Move INTAKE line to MillQ water" @16:02:54.07 Answered Move INTAKE line to MillQ water"" @16:02:54.10 Toroid.seek :bypassed @16:02:54.24 Priming sample loop w/50ml, bypass w/25ml @16:02:54.26 SamplePump.setPosition! 0ml @16:02:54.34 Bypass.open @16:02:56.13 Intake.open @16:02:57.84 Abs.in :default @16:02:57.92 Abs.hold @16:02:58.19 SamplePump.in :start @16:02:58.27 SamplePump.seek 25ml,2:10 @16:02:58.88 SamplePump.configure :prime @16:03:28.31 Abs.stop @16:03:28.40 SamplePump.setPosition! 0ml @16:03:28.51 Exhaust.open @16:03:30.08 Bypass.close @16:03:31.78 Abs.in :default @16:03:31.86 Abs.hold @16:03:32.19 SamplePump.in :start @16:03:32.27 SamplePump.seek 50ml,3:50 @16:03:32.88 SamplePump.configure :prime @16:04:31.92 Abs.stop @16:04:32.42 Toroid.seek :cartridge @16:04:36.90 Sampling 25.0ml @16:04:36.93 SamplePump.setPosition! 0ml @16:04:37.21 Abs.in :default @16:04:37.29 Abs.hold @16:04:37.61 SamplePump.in :start @16:04:37.71 SamplePump.seek 25ml,2:35 @16:04:38.30 SamplePump.configure :sample @16:05:07.76 Abs.stop @16:05:07.84 Intake.close @16:05:07.89 Exhaust.close @16:05:08.03 Toroid.seek :bypassed @16:05:12.47 Sampled 25.0ml @16:05:12.75 Air.in :unlocking @16:05:12.88 Air.seek :clear @16:05:13.90 Lysis1.in :unlocking @16:05:14.05 Lysis1.seek :clear @16:05:14.16 Plunge.in :unlocking @16:05:14.24 Plunge.seek :clear @16:05:15.39 Portable sample Path clean, ready for next sample @16:14:37.77 -> Cart.fire @16:14:37.89 Lysis1.in :fast @16:14:37.97 Lysis1.seek :locked @16:14:40.56 Plunge.in :default @16:14:40.63 Plunge.seek :locked @16:14:40.84 Air.in :fast @16:14:40.92 Air.seek :locked @16:14:41.91 Powering cartridge bus @16:14:44.59 PuckHeater.configure :default @16:14:44.68 Gate.power :heat,:ON,limit to 5000mA @16:14:45.42 Mounted lysis Cartridge board#J59964 @16:14:45.47 Using default cartridge heat loss curve for HeatingFunctionInStage @16:14:45.72 Air.in :default @16:14:45.76 Toroid.seek :bypassed @16:14:45.90 Air.seek :sealed @16:14:46.05 Priming sample loop w/25ml, bypass w/10ml @16:14:46.07 SamplePump.setPosition! 0ml @16:14:46.16 Bypass.open @16:14:47.81 Intake.open @16:14:48.79 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:14:51.40 Vacuum tare is 51% of full scale at 22C (drifted -0%) @16:14:51.59 Abs.in :default @16:14:51.68 Abs.hold @16:14:52.48 SamplePump.in :start @16:14:52.58 SamplePump.seek 10ml,1:10 @16:14:53.16 SamplePump.configure :prime @16:15:04.91 Abs.stop @16:15:05.00 SamplePump.setPosition! 0ml @16:15:05.07 Exhaust.open @16:15:06.66 Bypass.close @16:15:08.41 Abs.in :default @16:15:08.51 Abs.hold @16:15:08.85 SamplePump.in :start @16:15:08.94 SamplePump.seek 25ml,2:10 @16:15:09.54 SamplePump.configure :prime @16:15:38.99 Abs.stop @16:15:39.07 Bypass.open @16:15:39.95 Intake.close @16:15:40.84 Toroid.seek :crack @16:15:45.13 Toroid.seek :cartridge @16:15:46.23 Pressurizing cartridge to 26psi above 12.7psia @16:15:46.26 SamplePump.setPosition! 0ml @16:15:46.36 Intake.open @16:15:47.30 Bypass.close @16:15:48.18 Exhaust.close @16:15:49.16 Abs.in :default @16:15:49.23 Abs.hold @16:15:49.50 SamplePump.in :charge @16:15:49.59 SamplePump.seek 2.3ml,40 seconds @16:16:05.04 Abs.stop @16:16:05.13 Intake.close @16:16:06.07 Pressurized cartridge with 1.097ml to 26.4psi @16:16:06.08 Isolated @38.6psia (25.9psi) @16:16:06.12 Verifying cartridge holds at least 0.65..0.85 of 38.6psia @16:16:06.18 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:16:10.81 Toroid.seek :cartridge @16:16:10.94 Sampling 1000.0ml @16:16:10.96 SamplePump.setPosition! 0ml @16:16:11.05 Exhaust.open @16:16:11.91 Intake.open @16:16:13.44 Abs.in :default @16:16:13.53 Abs.hold @16:16:13.95 SamplePump.in :start @16:16:14.05 SamplePump.seek 1000ml,1:07:10 @16:16:14.64 SamplePump.configure :sample @16:16:17.04 filtering 2/1000ml at 0.84ml/s with 17.9psi vacuum @16:17:17.10 filtering 50/1000ml at 0.79ml/s with 24.9psi vacuum @16:18:17.09 filtering 98/1000ml at 0.81ml/s with 19.9psi vacuum @16:19:17.09 filtering 147/1000ml at 0.81ml/s with 21.0psi vacuum @16:20:17.09 filtering 195/1000ml at 0.81ml/s with 25.5psi vacuum @16:21:17.09 filtering 244/1000ml at 0.80ml/s with 20.9psi vacuum @16:22:17.10 filtering 292/1000ml at 0.80ml/s with 23.8psi vacuum @16:23:17.09 filtering 339/1000ml at 0.80ml/s with 17.3psi vacuum @16:24:17.11 filtering 387/1000ml at 0.79ml/s with 26.0psi vacuum @16:25:17.09 filtering 434/1000ml at 0.79ml/s with 23.8psi vacuum @16:26:17.08 filtering 481/1000ml at 0.78ml/s with 23.6psi vacuum @16:27:17.09 filtering 528/1000ml at 0.78ml/s with 24.3psi vacuum @16:28:17.10 filtering 575/1000ml at 0.78ml/s with 24.4psi vacuum @16:29:17.10 filtering 622/1000ml at 0.78ml/s with 23.8psi vacuum @16:30:17.09 filtering 668/1000ml at 0.78ml/s with 22.5psi vacuum @16:31:17.10 filtering 715/1000ml at 0.78ml/s with 24.8psi vacuum @16:32:17.10 filtering 761/1000ml at 0.78ml/s with 24.7psi vacuum @16:33:17.10 filtering 808/1000ml at 0.78ml/s with 20.8psi vacuum @16:34:17.09 filtering 855/1000ml at 0.78ml/s with 21.8psi vacuum @16:35:17.10 filtering 901/1000ml at 0.78ml/s with 21.2psi vacuum @16:36:17.10 filtering 948/1000ml at 0.78ml/s with 21.6psi vacuum @16:37:17.09 filtering 994/1000ml at 0.78ml/s with 24.8psi vacuum @16:37:24.20 Abs.stop @16:37:24.30 Intake.close @16:37:24.39 Exhaust.close @16:37:24.55 Toroid.seek :bypassed @16:37:28.92 Sampled 1000.0ml @16:37:28.97 Toroid.seek :bypassed @16:37:29.07 delay 5 seconds @16:37:34.10 Handoff.select :waste @16:37:34.26 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:37:41.48 Syringe tare is 51% of full scale at 22C (drifted -0%) @16:37:41.52 PinPort.in :locking @16:37:41.60 PinPort.seek :locked @16:37:42.03 Archive.in :fast @16:37:42.14 Lysis2.in :fast @16:37:42.22 Lysis1.seek 1250uL @16:37:42.29 Archive.seek :empty @16:37:42.48 Lysis2.seek :empty @16:37:50.88 Evacuating puck with filter bubble point of 50psi @16:37:50.93 PinPort.seek :locked @16:37:51.04 Handoff.select :waste @16:37:51.09 Plunge.seek :puckBottom @16:37:52.48 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:37:59.08 Air syringe pumping up to 1 strokes to reach 8psi @16:37:59.09 Air stroke #1 of 1 @16:37:59.22 Air.in :pressurize @16:37:59.31 Air.seek :pressurized @16:38:04.67 0.1psi after pressurize @16:38:04.69 Air.in :compress @16:38:04.82 Air.seek :evac @16:38:12.50 -0.0psi after compress @16:38:23.10 -0.0psi after compress (stable) @16:38:23.12 Air.in :default @16:38:23.22 Air.hold @16:38:23.53 at 0.1psi Opened to waste @16:38:23.55 Handoff.select :blocked @16:38:23.73 Plunge.seek :puckTop @16:38:24.65 Air syringe pumping up to 8 strokes to reach 50psi @16:38:24.66 Air stroke #1 of 8 @16:38:24.81 Air.in :compress @16:38:24.89 Air.seek :evac @16:38:25.07 15.1psi after compress @16:38:35.66 15.3psi after compress (stable) @16:38:35.69 Air.in :gulp @16:38:35.82 Air.seek :topVent @16:38:42.50 15.3psi after gulp @16:38:48.09 15.4psi after gulp (stable) @16:38:48.11 Air stroke #2 of 8 @16:38:48.25 Air.in :pressurize @16:38:48.33 Air.seek :pressurized @16:38:55.16 15.2psi after pressurize @16:38:55.20 Air.in :compress @16:38:55.30 Air.seek :evac @16:39:02.89 33.1psi after compress @16:39:13.98 35.6psi after compress (stable) @16:39:14.02 Air.in :gulp @16:39:14.12 Air.seek :topVent @16:39:20.77 35.5psi after gulp @16:39:26.36 35.5psi after gulp (stable) @16:39:26.38 Air stroke #3 of 8 @16:39:26.56 Air.in :pressurize @16:39:26.64 Air.seek :pressurized @16:39:32.98 35.5psi after pressurize @16:39:33.01 Air.in :compress @16:39:33.12 Air.seek :evac @16:39:40.69 Reached 50.9 of 50.0psi after pumping 5427uL (3.00 strokes) @16:39:40.71 Air.in :default @16:39:40.81 Air.hold @16:39:40.91 Plunge.seek :puckBottom @16:39:41.82 delay 3 seconds @16:39:44.84 Handoff.select :waste @16:39:45.00 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:39:52.09 delay 1:00 @16:40:52.13 Handoff.select :blocked @16:40:52.29 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @16:41:14.88 Handoff.select :waste @16:41:15.03 1.4psi at evacEnd @16:41:15.04 Handoff.select :blocked @16:41:15.22 Plunge.seek :puckTop @16:41:16.12 Air.in :fast @16:41:16.21 Air.seek :topVent @16:41:22.91 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @16:41:29.50 Stable @34.9psi after Handoff blocked @16:41:29.54 Air syringe pumping up to 8 strokes to reach 35psi @16:41:29.56 Air stroke #1 of 8 @16:41:29.71 Air.in :pressurize @16:41:29.79 Air.seek :pressurized @16:41:30.01 Reached 35.1 of 35.0psi after pumping 0uL (0.00 strokes) @16:41:30.03 Air.in :default @16:41:30.12 Air.hold @16:41:30.24 Plunge.seek :puckBottom @16:41:31.19 delay 3 seconds @16:41:34.21 Handoff.select :waste @16:41:34.38 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:41:49.48 delay 1:00 @16:42:49.51 Handoff.select :blocked @16:42:49.68 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @16:43:12.27 Handoff.select :waste @16:43:12.45 0.7psi at evacEnd @16:43:12.48 Plunge.seek :pTop @16:43:13.40 delay 5 seconds @16:43:18.44 Air.in :glide @16:43:18.54 Air.seek :fullVent @16:43:36.06 delay 2 seconds @16:43:38.09 Plunge.seek :pBottom @16:43:38.99 Seawater evacuated, Pressure above/below filter released. @16:43:39.02 Plunge.seek :locked @16:43:40.47 Handoff.select :waste @16:43:40.60 Lysis1.in :slow @16:43:40.69 Lysis1.seek 950uL @16:43:54.77 delay 5 seconds @16:43:59.80 Handoff.select :blocked @16:43:59.97 Plunge.seek :puckTop @16:44:01.01 Lysis1.seek 350uL @16:44:28.99 delay 3 seconds @16:44:32.09 Air.in mix with 5.31227 parts Lysis1 @16:44:32.84 Air.seek :evac @16:44:32.89 Lysis1.seek 190uL @16:44:40.60 Air.in :fast @16:44:40.69 Air.seek :full @16:44:47.38 Lysis1.seek :empty @16:44:56.53 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @17.2psi @16:44:56.56 Plunge.seek :puckBottom @16:44:57.61 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @16.3psi @16:44:57.63 Polling Can every 2:00 @16:44:58.20 PuckHeater.configure :default #ambient 22.2C @16:44:58.28 PuckHeater.seek 70.4C @16:46:06.49 PuckHeater.hold 2:00,70.4C @16:48:06.22 Polling Can every 2:00 @16:48:06.77 PuckHeater.seek 69.4C @16:48:06.99 PuckHeater.hold 2:00,69.4C @16:50:06.66 Polling Can every 2:00 @16:50:07.21 PuckHeater.configure :default #ambient 22.1C @16:50:07.30 PuckHeater.hold 18:00,68.3C @17:08:03.10 Polling Can every 2:00 @17:08:03.65 PuckHeater.configure :default #ambient 22.2C @17:08:03.73 PuckHeater.seek 104.3C @17:09:04.32 PuckHeater.hold 2:00,104.3C @17:11:03.95 Polling Can every 2:00 @17:11:04.50 PuckHeater.configure :default #ambient 22.2C @17:11:04.59 PuckHeater.seek 103.3C @17:11:04.79 PuckHeater.hold 2:00,103.3C @17:13:04.33 Polling Can every 2:00 @17:13:04.88 PuckHeater.configure :default #ambient 22.2C @17:13:04.97 PuckHeater.hold 2:00,102.2C @17:15:04.62 delay 5:00 @17:20:04.70 Lysate cooked @9.3psi @17:20:04.72 Plunge.seek :puckTop @17:20:05.65 Air syringe pumping up to 5 strokes to reach 40psi @17:20:05.66 Air stroke #1 of 5 @17:20:05.80 Air.in :pressurize @17:20:05.87 Air.seek :pressurized @17:20:12.22 9.8psi after pressurize @17:20:12.25 Air.in :compress @17:20:12.35 Air.seek :evac @17:20:17.58 Reached 41.5 of 40.0psi after pumping 1672uL (0.92 strokes) @17:20:17.60 Air.in :default @17:20:17.71 Air.hold @17:20:17.82 delay 2 seconds @17:20:19.85 Air.in :fast @17:20:19.93 Air.seek :full @17:20:26.10 Plunge.seek :puckBottom @17:20:27.08 Lysis1.seek 138uL @17:20:33.66 delay 2 seconds @17:20:35.69 Plunge.seek :puckTop @17:20:36.62 Lysis1.seek :empty @17:20:43.21 Plunge.seek :puckBottom @17:20:44.31 Lysis1.in :extract @17:20:44.46 Lysis1.seek 675uL,nil @17:20:46.92 Air.in :pressurize @17:20:47.01 Air.seek :pressurized @17:20:53.37 10.6psi after pressurize @17:20:53.40 Air.in :compressLysate @17:20:53.52 Air.seek :evac @17:21:16.21 Air.in :default @17:21:16.28 Air.hold @17:21:16.66 Pulled 675uL lysate @14.6psi @17:21:16.88 Lysis1.seek 800uL,nil @17:21:17.13 Air.in :compressLysate @17:21:17.24 Air.seek :evac @17:21:17.70 Reached 15.6 of 15.5psi after pumping 1715uL (0.95 strokes) @17:21:17.72 Air.in :default @17:21:17.81 Air.hold @17:21:18.27 Air.in :compressLysate @17:21:18.35 Air.seek :evac @17:21:25.31 12.4psi after compress @17:21:35.90 12.8psi after compress (stable) @17:21:41.08 Air.in :default @17:21:41.24 Air.hold @17:21:41.33 Lysis1.in :default @17:21:41.44 Lysis1.hold @17:21:41.67 Air.in :glide @17:21:41.76 Air.seek :fullVent @17:21:42.98 Handoff.select :waste @17:21:43.12 Recovered 748uL lysate @12.8psi @17:21:43.14 delay 3 seconds @17:21:46.23 After 3 delay Recovered 748uL lysate @-0.1psi @17:21:46.26 delay 5 seconds @17:21:51.31 Gate.power :heat,:OFF @17:21:51.34 Gate.power :cartridge,:OFF @17:21:51.46 PinPort.in :unlocking @17:21:51.54 PinPort.seek :clear @17:21:52.04 Archive.in :unlocking @17:21:52.18 Archive.seek :clear @17:21:52.30 Air.in :unlocking @17:21:52.47 Air.seek :clear @17:21:52.60 Lysis2.in :unlocking @17:21:52.68 Lysis2.seek :clear @17:21:57.42 Lysis1.in :unlocking @17:21:57.59 Lysis1.seek :clear @17:21:57.67 Plunge.in :unlocking @17:21:57.79 Plunge.seek :clear @09:53:37.10PDT04-Sep-25 -> #Cartridge71 @09:53:42.50 -> exit @09:53:42.54 Session Ended @10:43:38.37PDT04-Sep-25
Environmental Sample Processor built 28-Aug-25 (08574b7e) @10:43:42.15 >BREAK< ESP3 Gateway boot v1.42 for core bus @10:43:42.17 >BREAK< ESP3 Gateway boot v1.42 for cartridge bus @10:43:45.91 Configuration for marin by nadia@mbari.org 8/18/2025 /home/esp/esp2/type/portable/marin/configure.rb @10:43:46.50 Sea pressure (Abs) tare is 5% of full scale @10:43:48.13 Polling Can every 2:00 @10:43:48.18 Waiting for espclients... @10:43:49.40 Session Begun @10:44:00.27 -> ESP.ready! @10:44:00.34 Gate.power :main,:ON,limit to 5000mA @10:44:00.36 Gate.power :analytic1,:ON,limit to 4000mA @10:44:00.40 Gate.power :analytic2,:ON,limit to 4000mA @10:44:00.44 Gate.power :analytic3,:ON,limit to 4000mA @10:44:00.52 Powering core bus @10:44:03.28 Plunge.configure :default @10:44:03.39 SamplePump.configure :default @10:44:03.51 Abs.configure :default @10:44:03.61 Toroid.configure :default @10:44:03.68 PinPort.configure :default @10:44:03.78 Archive.configure :default @10:44:03.88 Air.configure :default @10:44:03.97 Lysis1.configure :default @10:44:04.05 Lysis2.configure :default @10:44:04.13 All elf controllers running firmware version 4.93 @10:44:04.17 Gate.power :motor,:ON,limit to 5000mA @10:44:04.41 Plunge.home.jog 0 @10:44:05.37 Lysis2.home.jog 0 @10:44:05.45 Lysis1.home.jog 0 @10:44:05.53 Archive.home.jog 0 @10:44:05.56 Toroid.home.jog 0 @10:44:05.60 Air.home.jog 0 @10:44:05.65 PinPort.home.jog 0 @10:44:06.39 Plunge.in :homing @10:44:06.69 Lysis1.in :homing @10:44:06.78 Air.in :homing @10:44:06.83 PinPort.in :homing @10:44:06.92 Toroid.in :homing @10:44:06.96 Lysis2.in :homing @10:44:07.01 Plunge.jog 549 @10:44:07.07 Archive.in :homing @10:44:07.37 Lysis1.jog 1667 @10:44:07.41 Air.jog 1568 @10:44:07.46 PinPort.jog -708 @10:44:07.51 Toroid.jog 263 @10:44:07.54 Lysis2.jog 1614 @10:44:07.60 Archive.jog 1622 @10:44:08.03 Plunge.setRawPosition! 50 @10:44:08.12 Plunge.seek :home @10:44:08.42 Plunge.in :default @10:44:08.72 Toroid.setRawPosition! 0 @10:44:08.80 Toroid.seek :home @10:44:08.95 PinPort.setRawPosition! -50 @10:44:08.97 Toroid.in :default @10:44:09.11 PinPort.seek :home @10:44:09.25 Lysis2.setRawPosition! 50 @10:44:09.30 Lysis1.setRawPosition! 50 @10:44:09.34 Air.setRawPosition! 50 @10:44:09.40 Archive.setRawPosition! 50 @10:44:09.61 Lysis2.seek :home @10:44:09.65 Lysis1.seek :home @10:44:09.69 Air.seek :home @10:44:09.75 Archive.seek :home @10:44:10.12 Lysis2.in :default @10:44:10.16 PinPort.in :default @10:44:10.35 Air.in :default @10:44:10.38 Archive.in :default @10:44:10.43 Lysis1.in :default @10:44:10.93 Toroid.seek :clear @10:44:11.10 PinPort.in :unlocking @10:44:11.18 PinPort.seek :clear @10:44:13.20 Air.seek :clear @10:44:13.88 Lysis1.seek :clear @10:44:14.80 Lysis2.seek :clear @10:44:15.61 Archive.seek :clear Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Abs = Absolute Pressure (Leak) Sensor @14psia Toroid = Toroid Valve (TV) clear PinPort = Handoff Pin Port (PP) clear Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear @10:44:21.37 -> ESP.shutdown @10:44:21.57 Gate.power :analytic3,:OFF @10:44:21.59 Gate.power :motor,:OFF @10:44:21.63 Gate.power :analytic1,:OFF @10:44:21.66 Gate.power :analytic2,:OFF @10:44:21.70 Gate.power :core,:OFF @10:44:21.74 Gate.power :main,:OFF [:analytic3,:motor,:analytic1,:analytic2,:core,:main] @16:10:47.85PDT06-Sep-25 -> ESP.ready! @16:10:47.91 Gate.power :main,:ON,limit to 5000mA @16:10:47.98 Gate.power :analytic1,:ON,limit to 4000mA @16:10:48.01 Gate.power :analytic2,:ON,limit to 4000mA @16:10:48.05 Gate.power :analytic3,:ON,limit to 4000mA @16:10:48.15 Powering core bus @16:10:50.92 Plunge.configure :default @16:10:51.03 SamplePump.configure :default @16:10:51.13 Abs.configure :default @16:10:51.24 Toroid.configure :default @16:10:51.32 PinPort.configure :default @16:10:51.41 Archive.configure :default @16:10:51.52 Air.configure :default @16:10:51.60 Lysis1.configure :default @16:10:51.67 Lysis2.configure :default @16:10:51.75 All elf controllers running firmware version 4.93 @16:10:51.78 Gate.power :motor,:ON,limit to 5000mA @16:10:52.09 Plunge.home.jog 0 @16:10:52.75 Lysis1.home.jog 0 @16:10:52.79 Air.home.jog 0 @16:10:52.84 Archive.home.jog 0 @16:10:52.92 Lysis2.home.jog 0 @16:10:52.97 Toroid.home.jog 0 @16:10:53.01 PinPort.home.jog 0 @16:10:54.41 Plunge.in :homing @16:10:54.77 Plunge.jog 454 @16:10:54.84 Lysis1.in :homing @16:10:54.90 Archive.in :homing @16:10:54.94 Toroid.in :homing @16:10:55.01 Lysis2.in :homing @16:10:55.03 PinPort.in :homing @16:10:55.07 Air.in :homing @16:10:55.38 Lysis1.jog 1660 @16:10:55.42 Archive.jog 1619 @16:10:55.61 Lysis2.jog 300 @16:10:55.66 PinPort.jog -720 @16:10:55.72 Toroid.jog 824 @16:10:55.83 Air.jog 1567 @16:10:56.15 Plunge.setRawPosition! 50 @16:10:56.23 Plunge.seek :home @16:10:56.54 Lysis2.setRawPosition! 50 @16:10:56.56 Plunge.in :default @16:10:56.74 Lysis2.seek :home @16:10:56.88 Lysis1.setRawPosition! 50 @16:10:56.96 Lysis1.seek :home @16:10:57.12 Archive.setRawPosition! 50 @16:10:57.14 PinPort.setRawPosition! -50 @16:10:57.19 Lysis2.in :default @16:10:57.37 Archive.seek :home @16:10:57.40 PinPort.seek :home @16:10:57.65 Air.setRawPosition! 50 @16:10:57.67 Lysis1.in :default @16:10:57.82 Air.seek :home @16:10:57.96 PinPort.in :default @16:10:57.99 Archive.in :default @16:10:58.19 Air.in :default @16:10:58.74 Toroid.setRawPosition! 0 @16:10:58.82 Toroid.seek :home @16:10:58.93 Toroid.in :default @16:10:59.02 Toroid.seek :clear @16:10:59.20 PinPort.in :unlocking @16:10:59.29 PinPort.seek :clear @16:11:01.30 Air.seek :clear @16:11:01.97 Lysis1.seek :clear @16:11:02.89 Lysis2.seek :clear @16:11:03.72 Archive.seek :clear Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Abs = Absolute Pressure (Leak) Sensor @16psia Toroid = Toroid Valve (TV) clear PinPort = Handoff Pin Port (PP) clear Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear @16:11:15.75 -> BACsampler.goalVolume 1000 @16:11:28.86 -> Cart.fire @16:11:28.98 Lysis1.in :fast @16:11:29.07 Lysis1.seek :locked @16:11:31.63 Plunge.seek :locked @16:11:31.82 Air.in :fast @16:11:31.89 Air.seek :locked @16:11:32.89 Powering cartridge bus @16:11:36.05 PuckHeater.configure :default @16:11:36.14 Gate.power :heat,:ON,limit to 5000mA @16:11:36.91 Mounted lysis Cartridge board#J59959 @16:11:36.97 Using default cartridge heat loss curve for HeatingFunctionInStage @16:11:37.23 Air.in :default @16:11:37.30 Toroid.seek :bypassed @16:11:37.35 Air.seek :sealed @16:11:39.61 Priming sample loop w/25ml, bypass w/10ml @16:11:39.63 SamplePump.setPosition! 0ml @16:11:39.72 Bypass.open @16:11:41.46 Intake.open @16:11:42.43 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:11:45.06 Vacuum tare is 51% of full scale at 23C (drifted -1%) @16:11:45.25 Abs.in :default @16:11:45.32 Abs.hold @16:11:45.60 SamplePump.in :start @16:11:45.69 SamplePump.seek 10ml,1:10 @16:11:46.29 SamplePump.configure :prime @16:11:57.96 Abs.stop @16:11:58.03 SamplePump.setPosition! 0ml @16:11:58.11 Exhaust.open @16:11:59.70 Bypass.close @16:12:01.40 Abs.in :default @16:12:01.47 Abs.hold @16:12:01.81 SamplePump.in :start @16:12:01.90 SamplePump.seek 25ml,2:10 @16:12:02.50 SamplePump.configure :prime @16:12:31.91 Abs.stop @16:12:31.99 Bypass.open @16:12:32.87 Intake.close @16:12:33.78 Toroid.seek :crack @16:12:38.06 Toroid.seek :cartridge @16:12:39.18 Pressurizing cartridge to 26psi above 12.9psia @16:12:39.21 SamplePump.setPosition! 0ml @16:12:39.29 Intake.open @16:12:40.19 Bypass.close @16:12:41.05 Exhaust.close @16:12:42.03 Abs.in :default @16:12:42.12 Abs.hold @16:12:42.82 SamplePump.in :charge @16:12:42.99 SamplePump.seek 2.3ml,40 seconds @16:12:58.76 Abs.stop @16:12:58.85 Intake.close @16:12:59.80 Pressurized cartridge with 1.139ml to 26.9psi @16:12:59.82 Isolated @37.9psia (25.0psi) @16:12:59.86 Verifying cartridge holds at least 0.65..0.85 of 37.9psia @16:12:59.92 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:13:04.55 Toroid.seek :cartridge @16:13:04.72 Sampling 1000.0ml @16:13:04.74 SamplePump.setPosition! 0ml @16:13:04.84 Exhaust.open @16:13:05.69 Intake.open @16:13:06.78 Abs.in :default @16:13:06.85 Abs.hold @16:13:07.21 SamplePump.in :start @16:13:07.31 SamplePump.seek 1000ml,1:07:10 @16:13:07.90 SamplePump.configure :sample @16:13:10.30 filtering 2/1000ml at 0.84ml/s with 16.2psi vacuum @16:14:10.36 filtering 52/1000ml at 0.84ml/s with 23.0psi vacuum @16:15:10.35 filtering 101/1000ml at 0.82ml/s with 22.5psi vacuum @16:16:10.34 filtering 150/1000ml at 0.81ml/s with 17.7psi vacuum @16:17:10.35 filtering 197/1000ml at 0.79ml/s with 23.7psi vacuum @16:18:10.35 filtering 244/1000ml at 0.78ml/s with 22.4psi vacuum @16:19:10.35 filtering 289/1000ml at 0.75ml/s with 25.3psi vacuum @16:20:10.35 filtering 333/1000ml at 0.73ml/s with 25.3psi vacuum @16:21:10.35 filtering 375/1000ml at 0.71ml/s with 25.3psi vacuum @16:22:10.35 filtering 416/1000ml at 0.68ml/s with 24.8psi vacuum @16:23:10.35 filtering 456/1000ml at 0.66ml/s with 23.9psi vacuum @16:24:10.35 filtering 494/1000ml at 0.64ml/s with 19.4psi vacuum @16:25:10.34 filtering 531/1000ml at 0.62ml/s with 24.1psi vacuum @16:26:10.35 filtering 567/1000ml at 0.60ml/s with 26.6psi vacuum @16:27:10.35 filtering 602/1000ml at 0.58ml/s with 22.3psi vacuum @16:28:10.35 filtering 636/1000ml at 0.57ml/s with 17.9psi vacuum @16:29:10.35 filtering 669/1000ml at 0.55ml/s with 25.8psi vacuum @16:30:10.79 filtering 701/1000ml at 0.53ml/s with 25.6psi vacuum @16:31:10.35 filtering 732/1000ml at 0.52ml/s with 21.3psi vacuum @16:32:10.35 filtering 762/1000ml at 0.50ml/s with 27.3psi vacuum @16:33:10.34 filtering 791/1000ml at 0.49ml/s with 18.6psi vacuum @16:34:10.35 filtering 820/1000ml at 0.48ml/s with 28.2psi vacuum @16:35:10.35 filtering 848/1000ml at 0.46ml/s with 29.6psi vacuum @16:36:10.35 filtering 875/1000ml at 0.45ml/s with 24.0psi vacuum @16:37:10.35 filtering 901/1000ml at 0.44ml/s with 26.8psi vacuum @16:38:10.35 filtering 927/1000ml at 0.43ml/s with 22.1psi vacuum @16:39:10.34 filtering 952/1000ml at 0.42ml/s with 22.3psi vacuum @16:40:10.35 filtering 976/1000ml at 0.41ml/s with 23.6psi vacuum @16:41:10.35 filtering 1000/1000ml at 0.40ml/s with 25.4psi vacuum @16:41:10.71 Abs.stop @16:41:10.83 Intake.close @16:41:10.91 Exhaust.close @16:41:11.05 Toroid.seek :bypassed @16:41:15.49 Sampled 1000.0ml @16:41:15.55 Toroid.seek :bypassed @16:41:15.67 delay 5 seconds @16:41:20.70 Handoff.select :waste @16:41:20.87 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:41:27.50 Syringe tare is 51% of full scale at 23C (drifted -0%) @16:41:27.54 PinPort.in :locking @16:41:27.65 PinPort.seek :locked @16:41:28.08 Archive.in :fast @16:41:28.20 Lysis2.in :fast @16:41:28.27 Lysis1.seek 1250uL @16:41:28.30 Archive.seek :empty @16:41:28.53 Lysis2.seek :empty @16:41:36.87 Evacuating puck with filter bubble point of 50psi @16:41:36.91 PinPort.seek :locked @16:41:37.06 Handoff.select :waste @16:41:37.10 Plunge.seek :puckBottom @16:41:38.50 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:41:45.18 Air syringe pumping up to 1 strokes to reach 8psi @16:41:45.19 Air stroke #1 of 1 @16:41:45.33 Air.in :pressurize @16:41:45.42 Air.seek :pressurized @16:41:50.78 0.0psi after pressurize @16:41:50.81 Air.in :compress @16:41:50.91 Air.seek :evac @16:41:58.52 0.9psi after compress @16:42:09.11 0.1psi after compress (stable) @16:42:09.13 Air.in :default @16:42:09.23 Air.hold @16:42:09.48 at 0.3psi Opened to waste @16:42:09.49 Handoff.select :blocked @16:42:09.68 Plunge.seek :puckTop @16:42:10.59 Air syringe pumping up to 8 strokes to reach 50psi @16:42:10.60 Air stroke #1 of 8 @16:42:10.78 Air.in :compress @16:42:10.87 Air.seek :evac @16:42:11.05 13.0psi after compress @16:42:21.64 13.3psi after compress (stable) @16:42:21.67 Air.in :gulp @16:42:21.77 Air.seek :topVent @16:42:28.41 13.4psi after gulp @16:42:34.00 13.3psi after gulp (stable) @16:42:34.01 Air stroke #2 of 8 @16:42:34.16 Air.in :pressurize @16:42:34.25 Air.seek :pressurized @16:42:40.61 13.3psi after pressurize @16:42:40.64 Air.in :compress @16:42:40.79 Air.seek :evac @16:42:48.40 31.0psi after compress @16:42:59.50 33.4psi after compress (stable) @16:42:59.53 Air.in :gulp @16:42:59.64 Air.seek :topVent @16:43:06.28 33.5psi after gulp @16:43:11.87 33.5psi after gulp (stable) @16:43:11.88 Air stroke #3 of 8 @16:43:12.03 Air.in :pressurize @16:43:12.11 Air.seek :pressurized @16:43:18.46 33.2psi after pressurize @16:43:18.48 Air.in :compress @16:43:18.59 Air.seek :evac @16:43:26.24 48.9psi after compress @16:43:26.53 Reached 50.0 of 50.0psi after pumping 5432uL (3.00 strokes) @16:43:26.55 Air.in :default @16:43:26.66 Air.hold @16:43:26.80 Plunge.seek :puckBottom @16:43:27.70 delay 3 seconds @16:43:30.72 Handoff.select :waste @16:43:30.88 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:43:41.98 delay 1:00 @16:44:42.01 Handoff.select :blocked @16:44:42.76 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @16:45:13.85 Handoff.select :waste @16:45:14.01 4.7psi at evacEnd @16:45:14.03 Handoff.select :blocked @16:45:14.21 Plunge.seek :puckTop @16:45:15.10 Air.in :fast @16:45:15.19 Air.seek :topVent @16:45:21.94 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @16:45:28.53 Stable @24.4psi after Handoff blocked @16:45:28.56 Air syringe pumping up to 8 strokes to reach 35psi @16:45:28.57 Air stroke #1 of 8 @16:45:28.77 Air.in :pressurize @16:45:28.86 Air.seek :pressurized @16:45:35.22 24.2psi after pressurize @16:45:35.26 Air.in :compress @16:45:35.37 Air.seek :evac @16:45:42.74 Reached 35.5 of 35.0psi after pumping 1792uL (0.99 strokes) @16:45:42.76 Air.in :default @16:45:42.86 Air.hold @16:45:42.96 Plunge.seek :puckBottom @16:45:43.87 delay 3 seconds @16:45:46.89 Handoff.select :waste @16:45:47.05 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:45:53.68 delay 1:00 @16:46:53.71 Handoff.select :blocked @16:46:53.98 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @16:47:16.57 Handoff.select :waste @16:47:16.73 0.5psi at evacEnd @16:47:16.76 Plunge.seek :pTop @16:47:17.68 delay 5 seconds @16:47:22.71 Air.in :glide @16:47:22.79 Air.seek :fullVent @16:47:24.33 delay 2 seconds @16:47:26.36 Plunge.seek :pBottom @16:47:27.25 Seawater evacuated, Pressure above/below filter released. @16:47:27.28 Plunge.seek :locked @16:47:29.16 Handoff.select :waste @16:47:29.28 Lysis1.in :slow @16:47:29.45 Lysis1.seek 950uL @16:47:43.53 delay 5 seconds @16:47:48.56 Handoff.select :blocked @16:47:48.72 Plunge.seek :puckTop @16:47:49.75 Lysis1.seek 350uL @16:48:17.74 delay 3 seconds @16:48:20.84 Air.in mix with 5.31227 parts Lysis1 @16:48:21.54 Air.seek :evac @16:48:21.63 Lysis1.seek 190uL @16:48:29.31 Air.in :fast @16:48:29.41 Air.seek :full @16:48:36.10 Lysis1.seek :empty @16:48:45.23 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @11.6psi @16:48:45.26 Plunge.seek :puckBottom @16:48:46.31 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @10.7psi @16:48:46.33 Polling Can every 2:00 @16:48:46.90 PuckHeater.configure :default #ambient 23.2C @16:48:46.97 PuckHeater.seek 70.4C @16:49:52.97 PuckHeater.hold 2:00,70.4C @16:51:52.73 Polling Can every 2:00 @16:51:53.28 PuckHeater.configure :default #ambient 23.2C @16:51:53.37 PuckHeater.seek 69.3C @16:51:53.59 PuckHeater.hold 2:00,69.3C @16:53:53.32 Polling Can every 2:00 @16:53:53.88 PuckHeater.configure :default #ambient 23.1C @16:53:53.96 PuckHeater.hold 18:00,68.2C @17:11:50.31 Polling Can every 2:00 @17:11:50.89 PuckHeater.configure :default #ambient 23.1C @17:11:50.97 PuckHeater.seek 104.3C @17:12:47.34 PuckHeater.hold 2:00,104.3C @17:14:47.04 Polling Can every 2:00 @17:14:47.60 PuckHeater.configure :default #ambient 23.3C @17:14:47.67 PuckHeater.seek 103.2C @17:14:47.88 PuckHeater.hold 2:00,103.2C @17:16:47.60 Polling Can every 2:00 @17:16:48.15 PuckHeater.configure :default #ambient 23.3C @17:16:48.24 PuckHeater.hold 2:00,102.2C @17:18:48.01 delay 5:00 @17:23:48.09 Lysate cooked @6.8psi @17:23:48.12 Plunge.seek :puckTop @17:23:49.04 Air syringe pumping up to 5 strokes to reach 40psi @17:23:49.05 Air stroke #1 of 5 @17:23:49.19 Air.in :pressurize @17:23:49.27 Air.seek :pressurized @17:23:55.62 6.7psi after pressurize @17:23:55.65 Air.in :compress @17:23:55.77 Air.seek :evac @17:24:01.78 Reached 41.3 of 40.0psi after pumping 1718uL (0.95 strokes) @17:24:01.80 Air.in :default @17:24:01.90 Air.hold @17:24:02.02 delay 2 seconds @17:24:04.05 Air.in :fast @17:24:04.12 Air.seek :full @17:24:10.49 Plunge.seek :puckBottom @17:24:11.47 Lysis1.seek 138uL @17:24:18.06 delay 2 seconds @17:24:20.08 Plunge.seek :puckTop @17:24:20.98 Lysis1.seek :empty @17:24:27.58 Plunge.seek :puckBottom @17:24:29.29 Lysis1.in :extract @17:24:29.37 Lysis1.seek 675uL,nil @17:24:31.93 Air.in :pressurize @17:24:32.02 Air.seek :pressurized @17:24:32.27 Syringe::LysatePlungerStuck in nadia -- at < 284uL during Lysis Syringe #1 (LyS1) move @17:24:32.32 Lysis1.in :default @17:24:32.41 Lysis1.seek 254uL @17:24:33.46 Reached 16.1 of 15.5psi after pumping 263uL (0.15 strokes) @17:24:33.48 Air.in :default @17:24:33.57 Air.hold @17:24:33.89 Lysis1.in :extract @17:24:33.97 Lysis1.seek 675uL,nil @17:24:35.16 Air.in :pressurize @17:24:35.26 Air.seek :pressurized @17:24:41.11 12.4psi after pressurize @17:24:41.16 Air.in :compressLysate @17:24:41.31 Air.seek :evac @17:24:57.15 Reached 16.1 of 15.5psi after pumping 1610uL (0.89 strokes) @17:24:57.17 Air.in :default @17:24:57.30 Air.hold @17:24:57.73 Air.in :compressLysate @17:24:57.81 Air.seek :evac @17:25:07.10 Air.in :default @17:25:07.17 Air.hold @17:25:07.49 Pulled 675uL lysate @14.3psi @17:25:07.70 Lysis1.seek 800uL,nil @17:25:07.96 Air.in :compressLysate @17:25:08.04 Air.seek :evac @17:25:13.09 13.0psi after compress @17:25:23.69 13.9psi after compress (stable) @17:25:28.86 Air.in :default @17:25:28.95 Air.hold @17:25:29.03 Lysis1.in :default @17:25:29.15 Lysis1.hold @17:25:29.38 Air.in :glide @17:25:29.47 Air.seek :fullVent @17:25:30.71 Handoff.select :waste @17:25:30.87 Recovered 689uL lysate @13.9psi @17:25:30.89 delay 3 seconds @17:25:34.00 After 3 delay Recovered 689uL lysate @-1.1psi @17:25:34.02 delay 5 seconds @17:25:39.07 Gate.power :heat,:OFF @17:25:39.10 Gate.power :cartridge,:OFF @17:25:39.24 PinPort.in :unlocking @17:25:39.33 PinPort.seek :clear @17:25:39.81 Archive.in :unlocking @17:25:39.98 Archive.seek :clear @17:25:40.11 Air.in :unlocking @17:25:40.27 Air.seek :clear @17:25:40.34 Lysis2.in :unlocking @17:25:40.47 Lysis2.seek :clear @17:25:45.20 Lysis1.in :unlocking @17:25:45.34 Lysis1.seek :clear @17:25:46.07 Plunge.in :unlocking @17:25:46.17 Plunge.seek :clear @17:42:36.88 -> cleanPortable @17:42:37.33 Waiting for nadia to answer "LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue" @17:43:32.52 Answered LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue"" @17:43:32.63 Lysis1.in :fast @17:43:32.75 Lysis1.seek :locked @17:43:35.31 Plunge.in :default @17:43:35.41 Plunge.seek :locked @17:43:35.60 Air.in :fast @17:43:35.69 Air.seek :locked @17:43:36.72 Powering cartridge bus @17:43:39.40 Gate.power :cartridge,:OFF @17:43:39.96 Mounted archive Cartridge @17:43:40.02 Toroid.seek :bypassed @17:43:40.12 Priming sample loop w/10ml, bypass w/10ml @17:43:40.14 SamplePump.setPosition! 0ml @17:43:40.24 Bypass.open @17:43:41.89 Intake.open @17:43:42.87 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:43:45.49 Vacuum tare is 51% of full scale at 23C (drifted -0%) @17:43:45.67 Abs.in :default @17:43:45.75 Abs.hold @17:43:46.01 SamplePump.in :start @17:43:46.10 SamplePump.seek 10ml,1:10 @17:43:46.70 SamplePump.configure :prime @17:43:58.38 Abs.stop @17:43:58.46 SamplePump.setPosition! 0ml @17:43:58.55 Exhaust.open @17:44:00.10 Bypass.close @17:44:01.81 Abs.in :default @17:44:01.88 Abs.hold @17:44:02.21 SamplePump.in :start @17:44:02.30 SamplePump.seek 10ml,1:10 @17:44:02.90 SamplePump.configure :prime @17:44:14.58 Abs.stop @17:44:14.70 Waiting for nadia to answer "INTAKE line plumbed to 10% Bleach, push return to continue" @18:25:53.91 Answered INTAKE line plumbed to 10% Bleach, push return to continue"" @18:25:53.94 Toroid.seek :bypassed @18:25:54.07 Priming sample loop w/10ml, bypass w/10ml @18:25:54.74 SamplePump.setPosition! 0ml @18:25:54.83 Exhaust.close @18:25:55.67 Bypass.open @18:25:57.50 Abs.in :default @18:25:57.59 Abs.hold @18:25:57.90 SamplePump.in :start @18:25:57.99 SamplePump.seek 10ml,1:10 @18:25:58.59 SamplePump.configure :prime @18:26:10.25 Abs.stop @18:26:10.35 SamplePump.setPosition! 0ml @18:26:10.42 Exhaust.open @18:26:11.97 Bypass.close @18:26:13.68 Abs.in :default @18:26:13.75 Abs.hold @18:26:14.09 SamplePump.in :start @18:26:14.18 SamplePump.seek 10ml,1:10 @18:26:14.78 SamplePump.configure :prime @18:26:26.45 Abs.stop @18:26:26.55 Toroid.seek :cartridge @18:26:30.90 Sampling 25.0ml @18:26:30.92 SamplePump.setPosition! 0ml @18:26:31.21 Abs.in :default @18:26:31.30 Abs.hold @18:26:31.63 SamplePump.in :start @18:26:31.73 SamplePump.seek 25ml,2:10 @18:26:32.32 SamplePump.configure :sample @18:27:01.74 Abs.stop @18:27:01.83 Intake.close @18:27:01.88 Exhaust.close @18:27:01.98 Handoff.select :close @18:27:02.08 Toroid.seek :bypassed @18:27:06.53 Sampled 25.0ml @18:27:06.56 delay 10:00 @18:37:06.61 Waiting for nadia to answer "Move INTAKE line to MillQ water" @18:39:51.69 Answered Move INTAKE line to MillQ water"" @18:39:51.71 Toroid.seek :bypassed @18:39:51.84 Priming sample loop w/50ml, bypass w/25ml @18:39:51.86 SamplePump.setPosition! 0ml @18:39:51.95 Bypass.open @18:39:53.71 Intake.open @18:39:55.42 Abs.in :default @18:39:55.50 Abs.hold @18:39:56.34 SamplePump.in :start @18:39:56.44 SamplePump.seek 25ml,2:10 @18:39:57.03 SamplePump.configure :prime @18:40:26.46 Abs.stop @18:40:26.54 SamplePump.setPosition! 0ml @18:40:26.63 Exhaust.open @18:40:28.21 Bypass.close @18:40:29.90 Abs.in :default @18:40:29.97 Abs.hold @18:40:30.31 SamplePump.in :start @18:40:30.41 SamplePump.seek 50ml,3:50 @18:40:31.00 SamplePump.configure :prime @18:41:30.00 Abs.stop @18:41:30.08 Toroid.seek :cartridge @18:41:34.44 Sampling 25.0ml @18:41:34.46 SamplePump.setPosition! 0ml @18:41:34.75 Abs.in :default @18:41:34.83 Abs.hold @18:41:35.16 SamplePump.in :start @18:41:35.25 SamplePump.seek 25ml,2:35 @18:41:35.85 SamplePump.configure :sample @18:42:05.27 Abs.stop @18:42:05.36 Intake.close @18:42:05.44 Exhaust.close @18:42:05.52 Toroid.seek :bypassed @18:42:09.95 Sampled 25.0ml @18:42:10.25 Air.in :unlocking @18:42:10.38 Air.seek :clear @18:42:11.38 Lysis1.in :unlocking @18:42:11.51 Lysis1.seek :clear @18:42:11.66 Plunge.in :unlocking @18:42:11.75 Plunge.seek :clear @18:42:12.84 Portable sample Path clean, ready for next sample @03:38:57.81PDT07-Sep-25 -> Cart.fire @03:38:57.94 Lysis1.in :fast @03:38:58.03 Lysis1.seek :locked @03:39:00.56 Plunge.in :default @03:39:00.73 Plunge.seek :locked @03:39:00.89 Air.in :fast @03:39:00.96 Air.seek :locked @03:39:01.96 Powering cartridge bus @03:39:04.64 PuckHeater.configure :default #ambient 23.3C @03:39:04.76 Gate.power :heat,:ON,limit to 5000mA @03:39:05.45 Mounted lysis Cartridge board#J60008 @03:39:05.51 Using default cartridge heat loss curve for HeatingFunctionInStage @03:39:05.77 Air.in :default @03:39:05.83 Toroid.seek :bypassed @03:39:05.92 Air.seek :sealed @03:39:06.09 Priming sample loop w/25ml, bypass w/10ml @03:39:06.11 SamplePump.setPosition! 0ml @03:39:06.20 Bypass.open @03:39:07.88 Intake.open @03:39:08.87 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @03:39:11.49 Vacuum tare is 51% of full scale at 21C (drifted -0%) @03:39:11.69 Abs.in :default @03:39:11.77 Abs.hold @03:39:12.03 SamplePump.in :start @03:39:12.78 SamplePump.seek 10ml,1:10 @03:39:12.88 SamplePump.configure :prime @03:39:24.89 Abs.stop @03:39:24.96 SamplePump.setPosition! 0ml @03:39:25.04 Exhaust.open @03:39:26.60 Bypass.close @03:39:28.34 Abs.in :default @03:39:28.42 Abs.hold @03:39:28.78 SamplePump.in :start @03:39:28.88 SamplePump.seek 25ml,2:10 @03:39:29.48 SamplePump.configure :prime @03:39:58.89 Abs.stop @03:39:58.98 Bypass.open @03:39:59.88 Intake.close @03:40:00.76 Toroid.seek :crack @03:40:05.05 Toroid.seek :cartridge @03:40:06.17 Pressurizing cartridge to 26psi above 13.7psia @03:40:06.19 SamplePump.setPosition! 0ml @03:40:06.27 Intake.open @03:40:07.17 Bypass.close @03:40:08.04 Exhaust.close @03:40:09.00 Abs.in :default @03:40:09.09 Abs.hold @03:40:09.32 SamplePump.in :charge @03:40:09.43 SamplePump.seek 2.3ml,40 seconds @03:40:25.28 Abs.stop @03:40:25.36 Intake.close @03:40:26.34 Pressurized cartridge with 1.158ml to 27.1psi @03:40:26.35 Isolated @40.6psia (26.9psi) @03:40:26.40 Verifying cartridge holds at least 0.65..0.85 of 40.6psia @03:40:26.46 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @03:40:31.08 Cartridge pressure rose 1% to 41.0psia @03:40:31.11 Toroid.seek :cartridge @03:40:31.24 Sampling 1000.0ml @03:40:31.26 SamplePump.setPosition! 0ml @03:40:31.36 Exhaust.open @03:40:32.84 Intake.open @03:40:33.89 Abs.in :default @03:40:33.97 Abs.hold @03:40:34.33 SamplePump.in :start @03:40:34.43 SamplePump.seek 1000ml,1:07:10 @03:40:35.02 SamplePump.configure :sample @03:40:37.41 filtering 2/1000ml at 0.84ml/s with 15.3psi vacuum @03:41:37.47 filtering 49/1000ml at 0.79ml/s with 24.3psi vacuum @03:42:37.47 filtering 94/1000ml at 0.75ml/s with 24.4psi vacuum @03:43:37.47 filtering 138/1000ml at 0.72ml/s with 24.5psi vacuum @03:44:37.47 filtering 180/1000ml at 0.70ml/s with 25.5psi vacuum @03:45:37.47 filtering 220/1000ml at 0.67ml/s with 22.1psi vacuum @03:46:37.47 filtering 260/1000ml at 0.66ml/s with 26.5psi vacuum @03:47:37.47 filtering 298/1000ml at 0.64ml/s with 22.2psi vacuum @03:48:37.48 filtering 336/1000ml at 0.63ml/s with 22.0psi vacuum @03:49:37.48 filtering 372/1000ml at 0.61ml/s with 23.6psi vacuum @03:50:37.47 filtering 408/1000ml at 0.60ml/s with 18.8psi vacuum @03:51:37.47 filtering 444/1000ml at 0.59ml/s with 23.7psi vacuum @03:52:37.48 filtering 478/1000ml at 0.58ml/s with 23.3psi vacuum @03:53:37.47 filtering 512/1000ml at 0.57ml/s with 18.4psi vacuum @03:54:37.52 filtering 546/1000ml at 0.56ml/s with 22.6psi vacuum @03:55:37.47 filtering 578/1000ml at 0.55ml/s with 22.8psi vacuum @03:56:37.46 filtering 611/1000ml at 0.55ml/s with 24.1psi vacuum @03:57:37.47 filtering 644/1000ml at 0.54ml/s with 22.2psi vacuum @03:58:37.47 filtering 676/1000ml at 0.53ml/s with 20.1psi vacuum @03:59:37.49 filtering 707/1000ml at 0.53ml/s with 21.8psi vacuum @04:00:37.47 filtering 738/1000ml at 0.52ml/s with 20.6psi vacuum @04:01:37.47 filtering 769/1000ml at 0.51ml/s with 26.6psi vacuum @04:02:37.47 filtering 799/1000ml at 0.50ml/s with 24.9psi vacuum @04:03:37.48 filtering 829/1000ml at 0.50ml/s with 24.5psi vacuum @04:04:37.47 filtering 859/1000ml at 0.50ml/s with 19.6psi vacuum @04:05:37.47 filtering 889/1000ml at 0.49ml/s with 28.0psi vacuum @04:06:37.46 filtering 917/1000ml at 0.48ml/s with 28.2psi vacuum @04:07:37.47 filtering 946/1000ml at 0.48ml/s with 18.6psi vacuum @04:08:37.98 filtering 974/1000ml at 0.47ml/s with 21.2psi vacuum @04:09:32.09 Abs.stop @04:09:32.20 Intake.close @04:09:32.26 Exhaust.close @04:09:32.38 Toroid.seek :bypassed @04:09:36.80 Sampled 1000.0ml @04:09:36.86 Toroid.seek :bypassed @04:09:36.97 delay 5 seconds @04:09:41.99 Handoff.select :waste @04:09:42.15 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @04:09:48.80 Syringe tare is 51% of full scale at 22C (drifted 0%) @04:09:48.84 PinPort.in :locking @04:09:48.96 PinPort.seek :locked @04:09:49.38 Archive.in :fast @04:09:49.45 Lysis2.in :fast @04:09:49.53 Lysis1.seek 1250uL @04:09:49.65 Archive.seek :empty @04:09:49.81 Lysis2.seek :empty @04:09:58.18 Evacuating puck with filter bubble point of 50psi @04:09:58.22 PinPort.seek :locked @04:09:58.37 Handoff.select :waste @04:09:58.42 Plunge.seek :puckBottom @04:09:59.82 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @04:10:06.42 Air syringe pumping up to 1 strokes to reach 8psi @04:10:06.43 Air stroke #1 of 1 @04:10:06.59 Air.in :pressurize @04:10:06.69 Air.seek :pressurized @04:10:12.07 0.2psi after pressurize @04:10:12.11 Air.in :compress @04:10:12.84 Air.seek :evac @04:10:20.46 1.2psi after compress @04:10:31.05 0.1psi after compress (stable) @04:10:31.07 Air.in :default @04:10:31.16 Air.hold @04:10:31.42 at 0.1psi Opened to waste @04:10:31.44 Handoff.select :blocked @04:10:31.62 Plunge.seek :puckTop @04:10:32.53 Air syringe pumping up to 8 strokes to reach 50psi @04:10:32.54 Air stroke #1 of 8 @04:10:32.73 Air.in :compress @04:10:32.82 Air.seek :evac @04:10:32.99 13.3psi after compress @04:10:43.60 13.5psi after compress (stable) @04:10:43.65 Air.in :gulp @04:10:43.76 Air.seek :topVent @04:10:50.42 13.7psi after gulp @04:10:56.01 13.6psi after gulp (stable) @04:10:56.03 Air stroke #2 of 8 @04:10:56.18 Air.in :pressurize @04:10:56.26 Air.seek :pressurized @04:11:02.61 13.7psi after pressurize @04:11:02.64 Air.in :compress @04:11:02.77 Air.seek :evac @04:11:10.38 31.5psi after compress @04:11:21.47 33.6psi after compress (stable) @04:11:21.50 Air.in :gulp @04:11:21.60 Air.seek :topVent @04:11:28.24 33.7psi after gulp @04:11:33.83 33.7psi after gulp (stable) @04:11:33.92 Air stroke #3 of 8 @04:11:34.07 Air.in :pressurize @04:11:34.16 Air.seek :pressurized @04:11:40.53 33.6psi after pressurize @04:11:40.56 Air.in :compress @04:11:40.70 Air.seek :evac @04:11:48.31 49.0psi after compress @04:11:48.53 Reached 49.9 of 50.0psi after pumping 5432uL (3.00 strokes) @04:11:48.55 Air.in :default @04:11:48.65 Air.hold @04:11:48.80 Plunge.seek :puckBottom @04:11:49.71 delay 3 seconds @04:11:52.73 Handoff.select :waste @04:11:52.90 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @04:12:03.00 delay 1:00 @04:13:03.03 Handoff.select :blocked @04:13:03.20 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @04:13:30.79 Handoff.select :waste @04:13:30.95 3.9psi at evacEnd @04:13:30.96 Handoff.select :blocked @04:13:31.13 Plunge.seek :puckTop @04:13:32.04 Air.in :fast @04:13:32.76 Air.seek :topVent @04:13:39.45 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @04:13:46.04 Stable @26.8psi after Handoff blocked @04:13:46.07 Air syringe pumping up to 8 strokes to reach 35psi @04:13:46.11 Air stroke #1 of 8 @04:13:46.25 Air.in :pressurize @04:13:46.33 Air.seek :pressurized @04:13:52.72 26.8psi after pressurize @04:13:52.75 Air.in :compress @04:13:52.84 Air.seek :evac @04:13:59.66 Reached 35.7 of 35.0psi after pumping 1765uL (0.97 strokes) @04:13:59.68 Air.in :default @04:13:59.78 Air.hold @04:13:59.90 Plunge.seek :puckBottom @04:14:00.81 delay 3 seconds @04:14:03.83 Handoff.select :waste @04:14:04.01 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @04:14:11.10 delay 1:00 @04:15:11.13 Handoff.select :blocked @04:15:11.29 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @04:15:33.88 Handoff.select :waste @04:15:34.03 1.0psi at evacEnd @04:15:34.06 Plunge.seek :pTop @04:15:34.98 delay 5 seconds @04:15:40.01 Air.in :glide @04:15:40.08 Air.seek :fullVent @04:15:42.00 delay 2 seconds @04:15:44.03 Plunge.seek :pBottom @04:15:44.93 Seawater evacuated, Pressure above/below filter released. @04:15:44.96 Plunge.seek :locked @04:15:46.37 Handoff.select :waste @04:15:46.50 Lysis1.in :slow @04:15:46.58 Lysis1.seek 950uL @04:16:00.67 delay 5 seconds @04:16:05.71 Handoff.select :blocked @04:16:05.89 Plunge.seek :puckTop @04:16:06.92 Lysis1.seek 350uL @04:16:34.91 delay 3 seconds @04:16:38.60 Air.in mix with 5.31227 parts Lysis1 @04:16:39.33 Air.seek :evac @04:16:39.43 Lysis1.seek 190uL @04:16:47.09 Air.in :fast @04:16:47.18 Air.seek :full @04:16:53.86 Lysis1.seek :empty @04:17:02.99 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @13.2psi @04:17:03.02 Plunge.seek :puckBottom @04:17:04.07 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @12.6psi @04:17:04.09 Polling Can every 2:00 @04:17:04.67 PuckHeater.configure :default #ambient 21.6C @04:17:04.79 PuckHeater.seek 70.5C @04:18:20.44 PuckHeater.hold 2:00,70.5C @04:20:20.09 Polling Can every 2:00 @04:20:20.65 PuckHeater.configure :default #ambient 21.7C @04:20:20.78 PuckHeater.seek 69.4C @04:20:21.01 PuckHeater.hold 2:00,69.4C @04:22:20.62 Polling Can every 2:00 @04:22:21.17 PuckHeater.configure :default #ambient 21.8C @04:22:21.26 PuckHeater.hold 18:00,68.3C @04:40:15.93 Polling Can every 2:00 @04:40:16.48 PuckHeater.configure :default #ambient 21.6C @04:40:16.56 PuckHeater.seek 104.3C @04:41:18.30 PuckHeater.hold 2:00,104.3C @04:43:17.74 Polling Can every 2:00 @04:43:18.29 PuckHeater.seek 103.3C @04:43:18.49 PuckHeater.hold 2:00,103.3C @04:45:18.00 Polling Can every 2:00 @04:45:18.55 PuckHeater.configure :default #ambient 21.6C @04:45:18.64 PuckHeater.hold 2:00,102.2C @04:47:18.25 delay 5:00 @04:52:18.33 Lysate cooked @11.9psi @04:52:18.36 Plunge.seek :puckTop @04:52:19.29 Air syringe pumping up to 5 strokes to reach 40psi @04:52:19.30 Air stroke #1 of 5 @04:52:20.06 Air.in :pressurize @04:52:20.17 Air.seek :pressurized @04:52:26.52 12.0psi after pressurize @04:52:26.55 Air.in :compress @04:52:26.68 Air.seek :evac @04:52:32.63 Reached 41.3 of 40.0psi after pumping 1714uL (0.95 strokes) @04:52:32.65 Air.in :default @04:52:32.81 Air.hold @04:52:32.92 delay 2 seconds @04:52:34.95 Air.in :fast @04:52:35.03 Air.seek :full @04:52:41.37 Plunge.seek :puckBottom @04:52:42.35 Lysis1.seek 138uL @04:52:48.93 delay 2 seconds @04:52:50.96 Plunge.seek :puckTop @04:52:51.87 Lysis1.seek :empty @04:52:58.47 Plunge.seek :puckBottom @04:52:59.54 Lysis1.in :extract @04:52:59.61 Lysis1.seek 675uL,nil @04:53:02.18 Air.in :pressurize @04:53:02.26 Air.seek :pressurized @04:53:02.50 Syringe::LysatePlungerStuck in nadia -- at < 283uL during Lysis Syringe #1 (LyS1) move @04:53:02.54 Lysis1.in :default @04:53:02.67 Lysis1.seek 253uL @04:53:04.14 Reached 15.6 of 15.5psi after pumping 367uL (0.20 strokes) @04:53:04.16 Air.in :default @04:53:04.26 Air.hold @04:53:04.72 Lysis1.in :extract @04:53:04.85 Lysis1.seek 675uL,nil @04:53:05.20 Air.in :pressurize @04:53:05.29 Air.seek :pressurized @04:53:10.07 12.1psi after pressurize @04:53:10.10 Air.in :compressLysate @04:53:10.21 Air.seek :evac @04:53:33.36 Air.in :default @04:53:33.45 Air.hold @04:53:33.76 Pulled 675uL lysate @15.1psi @04:53:33.96 Lysis1.seek 800uL,nil @04:53:36.05 Air.in :compressLysate @04:53:36.17 Air.seek :evac @04:53:42.88 12.6psi after compress @04:53:53.47 13.1psi after compress (stable) @04:53:58.70 Air.in :default @04:53:58.78 Lysis1.in :default @04:53:58.82 Air.hold @04:53:58.98 Lysis1.hold @04:53:59.82 Air.in :glide @04:53:59.95 Air.seek :fullVent @04:54:01.19 Handoff.select :waste @04:54:01.35 Recovered 734uL lysate @13.1psi @04:54:01.37 delay 3 seconds @04:54:04.47 After 3 delay Recovered 734uL lysate @-1.3psi @04:54:04.49 delay 5 seconds @04:54:09.55 Gate.power :heat,:OFF @04:54:09.57 Gate.power :cartridge,:OFF @04:54:09.71 PinPort.in :unlocking @04:54:09.79 PinPort.seek :clear @04:54:10.28 Archive.in :unlocking @04:54:10.46 Archive.seek :clear @04:54:10.59 Air.in :unlocking @04:54:10.80 Air.seek :clear @04:54:10.85 Lysis2.in :unlocking @04:54:11.00 Lysis2.seek :clear @04:54:15.73 Lysis1.in :unlocking @04:54:15.93 Lysis1.seek :clear @04:54:16.04 Plunge.in :unlocking @04:54:16.12 Plunge.seek :clear @05:05:26.01 -> cleanPortable @05:05:26.52 Waiting for nadia to answer "LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue" @05:05:29.58 Answered LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue"" @05:05:29.69 Lysis1.in :fast @05:05:29.78 Lysis1.seek :locked @05:05:32.35 Plunge.in :default @05:05:32.47 Plunge.seek :locked @05:05:32.61 Air.in :fast @05:05:32.73 Air.seek :locked @05:05:33.71 Powering cartridge bus @05:05:36.38 Gate.power :cartridge,:OFF @05:05:36.95 Mounted archive Cartridge @05:05:37.00 Toroid.seek :bypassed @05:05:37.10 Priming sample loop w/10ml, bypass w/10ml @05:05:37.13 SamplePump.setPosition! 0ml @05:05:37.22 Bypass.open @05:05:38.90 Intake.open @05:05:39.85 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @05:05:42.48 Vacuum tare is 52% of full scale at 22C (drifted 0%) @05:05:42.66 Abs.in :default @05:05:42.77 Abs.hold @05:05:43.63 SamplePump.in :start @05:05:43.72 SamplePump.seek 10ml,1:10 @05:05:44.32 SamplePump.configure :prime @05:05:55.98 Abs.stop @05:05:56.07 SamplePump.setPosition! 0ml @05:05:56.16 Exhaust.open @05:05:57.71 Bypass.close @05:05:59.43 Abs.in :default @05:05:59.52 Abs.hold @05:05:59.85 SamplePump.in :start @05:05:59.94 SamplePump.seek 10ml,1:10 @05:06:00.54 SamplePump.configure :prime @05:06:12.20 Abs.stop @05:06:12.28 Waiting for nadia to answer "INTAKE line plumbed to 10% Bleach, push return to continue" @05:06:24.30 Answered INTAKE line plumbed to 10% Bleach, push return to continue"" @05:06:24.33 Toroid.seek :bypassed @05:06:24.46 Priming sample loop w/10ml, bypass w/10ml @05:06:24.48 SamplePump.setPosition! 0ml @05:06:24.57 Exhaust.close @05:06:25.42 Bypass.open @05:06:27.21 Abs.in :default @05:06:27.30 Abs.hold @05:06:27.55 SamplePump.in :start @05:06:27.64 SamplePump.seek 10ml,1:10 @05:06:28.24 SamplePump.configure :prime @05:06:39.90 Abs.stop @05:06:39.98 SamplePump.setPosition! 0ml @05:06:40.06 Exhaust.open @05:06:41.64 Bypass.close @05:06:43.33 Abs.in :default @05:06:43.40 Abs.hold @05:06:43.72 SamplePump.in :start @05:06:43.81 SamplePump.seek 10ml,1:10 @05:06:44.41 SamplePump.configure :prime @05:06:56.07 Abs.stop @05:06:56.16 Toroid.seek :cartridge @05:07:00.51 Sampling 25.0ml @05:07:00.53 SamplePump.setPosition! 0ml @05:07:00.83 Abs.in :default @05:07:00.91 Abs.hold @05:07:01.86 SamplePump.in :start @05:07:01.96 SamplePump.seek 25ml,2:10 @05:07:02.55 SamplePump.configure :sample @05:07:31.96 Abs.stop @05:07:32.04 Intake.close @05:07:32.13 Exhaust.close @05:07:32.20 Handoff.select :close @05:07:32.34 Toroid.seek :bypassed @05:07:36.75 Sampled 25.0ml @05:07:36.78 delay 10:00 @05:17:36.83 Waiting for nadia to answer "Move INTAKE line to MillQ water" @05:17:36.87 Answered Move INTAKE line to MillQ water"" @05:17:36.90 Toroid.seek :bypassed @05:17:37.04 Priming sample loop w/50ml, bypass w/25ml @05:17:37.06 SamplePump.setPosition! 0ml @05:17:37.14 Bypass.open @05:17:38.90 Intake.open @05:17:40.63 Abs.in :default @05:17:40.74 Abs.hold @05:17:41.06 SamplePump.in :start @05:17:41.15 SamplePump.seek 25ml,2:10 @05:17:41.76 SamplePump.configure :prime @05:18:11.17 Abs.stop @05:18:11.24 SamplePump.setPosition! 0ml @05:18:11.37 Exhaust.open @05:18:12.94 Bypass.close @05:18:14.65 Abs.in :default @05:18:14.76 Abs.hold @05:18:15.11 SamplePump.in :start @05:18:15.20 SamplePump.seek 50ml,3:50 @05:18:15.80 SamplePump.configure :prime @05:19:14.80 Abs.stop @05:19:14.88 Toroid.seek :cartridge @05:19:19.24 Sampling 25.0ml @05:19:19.27 SamplePump.setPosition! 0ml @05:19:19.54 Abs.in :default @05:19:19.61 Abs.hold @05:19:19.96 SamplePump.in :start @05:19:20.06 SamplePump.seek 25ml,2:35 @05:19:20.65 SamplePump.configure :sample @05:19:50.07 Abs.stop @05:19:50.16 Intake.close @05:19:50.26 Exhaust.close @05:19:50.37 Toroid.seek :bypassed @05:19:55.43 Sampled 25.0ml @05:19:55.73 Air.in :unlocking @05:19:55.87 Air.seek :clear @05:19:56.87 Lysis1.in :unlocking @05:19:57.02 Lysis1.seek :clear @05:19:57.14 Plunge.in :unlocking @05:19:57.22 Plunge.seek :clear @05:19:58.32 Portable sample Path clean, ready for next sample @14:13:59.63 -> ESP.reready! @14:13:59.68 Plunge.in :default @14:13:59.80 Toroid.in :default @14:13:59.88 PinPort.in :default @14:13:59.94 Archive.in :default @14:14:00.09 Air.in :default @14:14:00.13 Plunge.home.jog 0 @14:14:00.18 Lysis1.in :default @14:14:00.30 Lysis2.in :default @14:14:00.38 Toroid.home.jog 0 @14:14:00.61 Archive.home.jog 0 @14:14:00.65 PinPort.home.jog 0 @14:14:00.79 Air.home.jog 0 @14:14:00.87 Lysis2.home.jog 0 @14:14:00.92 Lysis1.home.jog 0 @14:14:01.69 Plunge.in :homing @14:14:02.49 Plunge.jog 776 @14:14:02.80 Lysis2.in :homing @14:14:02.86 Lysis1.in :homing @14:14:02.90 Archive.in :homing @14:14:02.94 Toroid.in :homing @14:14:02.98 Air.in :homing @14:14:03.02 PinPort.in :homing @14:14:03.30 Lysis2.jog 1614 @14:14:03.35 Lysis1.jog 1662 @14:14:03.40 Archive.jog 1617 @14:14:03.46 Toroid.jog 265 @14:14:03.52 Air.jog 1568 @14:14:03.66 PinPort.jog -716 @14:14:04.04 Plunge.setRawPosition! 50 @14:14:04.11 Plunge.seek :home @14:14:04.42 Plunge.in :default @14:14:04.79 Toroid.setRawPosition! 0 @14:14:04.87 Toroid.seek :home @14:14:05.02 Lysis2.setRawPosition! 50 @14:14:05.08 Lysis1.setRawPosition! 50 @14:14:05.12 PinPort.setRawPosition! -50 @14:14:05.15 Toroid.in :default @14:14:05.40 Lysis2.seek :home @14:14:05.42 Lysis1.seek :home @14:14:05.47 PinPort.seek :home @14:14:05.78 Archive.setRawPosition! 50 @14:14:05.81 PinPort.in :default @14:14:05.86 Air.setRawPosition! 50 @14:14:06.03 Archive.seek :home @14:14:06.08 Air.seek :home @14:14:06.32 Lysis1.in :default @14:14:06.35 Lysis2.in :default @14:14:06.56 Air.in :default @14:14:06.58 Archive.in :default @14:14:07.04 Toroid.seek :clear @14:14:07.22 PinPort.in :unlocking @14:14:07.32 PinPort.seek :clear @14:14:09.36 Air.seek :clear @14:14:10.05 Lysis1.seek :clear @14:14:10.97 Lysis2.seek :clear @14:14:11.77 Archive.seek :clear Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 25ml Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Abs = Absolute Pressure (Leak) Sensor @16psia Toroid = Toroid Valve (TV) clear PinPort = Handoff Pin Port (PP) clear Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear @14:14:16.90 -> Cart.fire @14:14:17.02 Lysis1.in :fast @14:14:17.11 Lysis1.seek :locked @14:14:19.67 Plunge.seek :locked @14:14:19.87 Air.in :fast @14:14:19.94 Air.seek :locked @14:14:20.94 Powering cartridge bus @14:14:23.94 PuckHeater.configure :default #ambient 21.6C @14:14:24.04 Gate.power :heat,:ON,limit to 5000mA @14:14:24.77 Mounted lysis Cartridge board#J59993 @14:14:24.83 Using default cartridge heat loss curve for HeatingFunctionInStage @14:14:25.09 Air.in :default @14:14:25.15 Toroid.seek :bypassed @14:14:25.22 Air.seek :sealed @14:14:27.51 Priming sample loop w/25ml, bypass w/10ml @14:14:27.53 SamplePump.setPosition! 0ml @14:14:27.63 Bypass.open @14:14:29.29 Intake.open @14:14:30.26 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:14:32.88 Vacuum tare is 52% of full scale at 22C (drifted -0%) @14:14:33.06 Abs.in :default @14:14:33.14 Abs.hold @14:14:33.41 SamplePump.in :start @14:14:33.51 SamplePump.seek 10ml,1:10 @14:14:34.10 SamplePump.configure :prime @14:14:45.77 Abs.stop @14:14:45.86 SamplePump.setPosition! 0ml @14:14:45.95 Exhaust.open @14:14:47.52 Bypass.close @14:14:49.24 Abs.in :default @14:14:49.31 Abs.hold @14:14:49.64 SamplePump.in :start @14:14:49.74 SamplePump.seek 25ml,2:10 @14:14:50.33 SamplePump.configure :prime @14:15:19.76 Abs.stop @14:15:19.83 Bypass.open @14:15:20.73 Intake.close @14:15:21.60 Toroid.seek :crack @14:15:25.89 Toroid.seek :cartridge @14:15:27.02 Pressurizing cartridge to 26psi above 12.9psia @14:15:27.04 SamplePump.setPosition! 0ml @14:15:27.13 Intake.open @14:15:28.02 Bypass.close @14:15:29.49 Exhaust.close @14:15:30.48 Abs.in :default @14:15:30.57 Abs.hold @14:15:30.85 SamplePump.in :charge @14:15:30.95 SamplePump.seek 2.3ml,40 seconds @14:15:46.80 Abs.stop @14:15:46.90 Intake.close @14:15:47.87 Pressurized cartridge with 1.152ml to 26.5psi @14:15:47.88 Isolated @39.3psia (26.4psi) @14:15:47.94 Verifying cartridge holds at least 0.65..0.85 of 39.3psia @14:15:48.00 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:15:52.61 Toroid.seek :cartridge @14:15:52.78 Sampling 1000.0ml @14:15:52.80 SamplePump.setPosition! 0ml @14:15:52.91 Exhaust.open @14:15:53.77 Intake.open @14:15:54.85 Abs.in :default @14:15:54.93 Abs.hold @14:15:55.27 SamplePump.in :start @14:15:55.37 SamplePump.seek 1000ml,1:07:10 @14:15:55.96 SamplePump.configure :sample @14:15:58.37 filtering 2/1000ml at 0.81ml/s with 22.4psi vacuum @14:16:58.43 filtering 52/1000ml at 0.83ml/s with 22.5psi vacuum @14:17:58.43 filtering 100/1000ml at 0.81ml/s with 25.3psi vacuum @14:18:58.42 filtering 147/1000ml at 0.78ml/s with 25.8psi vacuum @14:19:58.43 filtering 193/1000ml at 0.76ml/s with 24.4psi vacuum @14:20:58.43 filtering 237/1000ml at 0.74ml/s with 17.3psi vacuum @14:21:58.44 filtering 280/1000ml at 0.72ml/s with 25.6psi vacuum @14:22:58.43 filtering 322/1000ml at 0.70ml/s with 19.4psi vacuum @14:23:58.42 filtering 363/1000ml at 0.68ml/s with 25.8psi vacuum @14:24:58.43 filtering 403/1000ml at 0.67ml/s with 23.2psi vacuum @14:25:59.04 filtering 442/1000ml at 0.65ml/s with 23.4psi vacuum @14:26:58.43 filtering 480/1000ml at 0.63ml/s with 20.6psi vacuum @14:27:58.43 filtering 517/1000ml at 0.61ml/s with 20.9psi vacuum @14:28:58.43 filtering 553/1000ml at 0.60ml/s with 14.5psi vacuum @14:29:58.43 filtering 588/1000ml at 0.59ml/s with 24.8psi vacuum @14:30:58.43 filtering 622/1000ml at 0.57ml/s with 27.0psi vacuum @14:31:58.43 filtering 656/1000ml at 0.56ml/s with 22.5psi vacuum @14:32:58.43 filtering 689/1000ml at 0.55ml/s with 21.2psi vacuum @14:33:58.43 filtering 722/1000ml at 0.54ml/s with 24.1psi vacuum @14:34:58.44 filtering 754/1000ml at 0.54ml/s with 22.5psi vacuum @14:35:58.43 filtering 786/1000ml at 0.53ml/s with 23.0psi vacuum @14:36:58.43 filtering 817/1000ml at 0.52ml/s with 24.4psi vacuum @14:37:58.43 filtering 848/1000ml at 0.51ml/s with 26.6psi vacuum @14:38:58.43 filtering 878/1000ml at 0.51ml/s with 26.2psi vacuum @14:39:58.43 filtering 908/1000ml at 0.50ml/s with 18.6psi vacuum @14:40:58.42 filtering 938/1000ml at 0.49ml/s with 19.0psi vacuum @14:41:58.43 filtering 967/1000ml at 0.49ml/s with 24.0psi vacuum @14:42:58.43 filtering 996/1000ml at 0.48ml/s with 22.9psi vacuum @14:43:06.98 Abs.stop @14:43:07.09 Intake.close @14:43:07.17 Exhaust.close @14:43:07.26 Toroid.seek :bypassed @14:43:11.70 Sampled 1000.0ml @14:43:11.76 Toroid.seek :bypassed @14:43:11.87 delay 5 seconds @14:43:16.89 Handoff.select :waste @14:43:17.05 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:43:23.67 Syringe tare is 51% of full scale at 23C (drifted 1%) @14:43:23.71 PinPort.in :locking @14:43:23.81 PinPort.seek :locked @14:43:24.23 Archive.in :fast @14:43:24.29 Lysis2.in :fast @14:43:24.38 Lysis1.seek 1250uL @14:43:24.54 Archive.seek :empty @14:43:25.36 Lysis2.seek :empty @14:43:33.76 Evacuating puck with filter bubble point of 50psi @14:43:33.79 PinPort.seek :locked @14:43:33.93 Handoff.select :waste @14:43:33.97 Plunge.seek :puckBottom @14:43:35.38 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:43:42.00 Air syringe pumping up to 1 strokes to reach 8psi @14:43:42.01 Air stroke #1 of 1 @14:43:42.17 Air.in :pressurize @14:43:42.26 Air.seek :pressurized @14:43:47.62 -0.0psi after pressurize @14:43:47.65 Air.in :compress @14:43:47.77 Air.seek :evac @14:43:55.37 0.4psi after compress @14:44:05.96 0.0psi after compress (stable) @14:44:05.98 Air.in :default @14:44:06.08 Air.hold @14:44:06.33 at -0.1psi Opened to waste @14:44:06.35 Handoff.select :blocked @14:44:07.15 Plunge.seek :puckTop @14:44:08.07 Air syringe pumping up to 8 strokes to reach 50psi @14:44:08.08 Air stroke #1 of 8 @14:44:08.23 Air.in :compress @14:44:08.32 Air.seek :evac @14:44:08.51 12.8psi after compress @14:44:19.10 12.9psi after compress (stable) @14:44:19.13 Air.in :gulp @14:44:19.24 Air.seek :topVent @14:44:25.88 12.9psi after gulp @14:44:31.47 12.9psi after gulp (stable) @14:44:31.48 Air stroke #2 of 8 @14:44:31.61 Air.in :pressurize @14:44:31.70 Air.seek :pressurized @14:44:38.05 13.0psi after pressurize @14:44:38.09 Air.in :compress @14:44:38.21 Air.seek :evac @14:44:45.81 30.9psi after compress @14:44:56.90 33.1psi after compress (stable) @14:44:56.94 Air.in :gulp @14:44:57.06 Air.seek :topVent @14:45:03.69 33.2psi after gulp @14:45:09.28 33.1psi after gulp (stable) @14:45:09.30 Air stroke #3 of 8 @14:45:09.43 Air.in :pressurize @14:45:09.53 Air.seek :pressurized @14:45:15.87 33.0psi after pressurize @14:45:15.89 Air.in :compress @14:45:16.00 Air.seek :evac @14:45:23.62 48.7psi after compress @14:45:23.86 Reached 49.7 of 50.0psi after pumping 5432uL (3.00 strokes) @14:45:23.89 Air.in :default @14:45:23.99 Air.hold @14:45:24.09 Plunge.seek :puckBottom @14:45:25.00 delay 3 seconds @14:45:28.02 Handoff.select :waste @14:45:28.18 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:45:41.77 delay 1:00 @14:46:41.81 Handoff.select :blocked @14:46:41.98 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:47:04.57 Handoff.select :waste @14:47:04.75 2.5psi at evacEnd @14:47:04.76 Handoff.select :blocked @14:47:04.99 Plunge.seek :puckTop @14:47:05.89 Air.in :fast @14:47:05.98 Air.seek :topVent @14:47:12.66 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @14:47:19.28 Stable @30.9psi after Handoff blocked @14:47:19.31 Air syringe pumping up to 8 strokes to reach 35psi @14:47:19.34 Air stroke #1 of 8 @14:47:19.48 Air.in :pressurize @14:47:19.57 Air.seek :pressurized @14:47:25.93 30.8psi after pressurize @14:47:25.96 Air.in :compress @14:47:26.07 Air.seek :evac @14:47:31.69 Reached 35.7 of 35.0psi after pumping 1695uL (0.94 strokes) @14:47:31.71 Air.in :default @14:47:31.81 Air.hold @14:47:31.92 Plunge.seek :puckBottom @14:47:32.83 delay 3 seconds @14:47:35.85 Handoff.select :waste @14:47:36.02 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:47:46.12 delay 1:00 @14:48:46.15 Handoff.select :blocked @14:48:46.32 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:49:08.91 Handoff.select :waste @14:49:09.10 1.3psi at evacEnd @14:49:09.14 Plunge.seek :pTop @14:49:10.05 delay 5 seconds @14:49:15.08 Air.in :glide @14:49:15.18 Air.seek :fullVent @14:49:17.79 delay 2 seconds @14:49:19.82 Plunge.seek :pBottom @14:49:20.73 Seawater evacuated, Pressure above/below filter released. @14:49:20.76 Plunge.seek :locked @14:49:22.16 Handoff.select :waste @14:49:22.28 Lysis1.in :slow @14:49:22.42 Lysis1.seek 950uL @14:49:36.49 delay 5 seconds @14:49:41.52 Handoff.select :blocked @14:49:41.69 Plunge.seek :puckTop @14:49:42.81 Lysis1.seek 350uL @14:50:10.80 delay 3 seconds @14:50:13.91 Air.in mix with 5.31227 parts Lysis1 @14:50:14.74 Air.seek :evac @14:50:14.79 Lysis1.seek 190uL @14:50:22.53 Air.in :fast @14:50:22.63 Air.seek :full @14:50:29.34 Lysis1.seek :empty @14:50:38.48 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @15.8psi @14:50:38.51 Plunge.seek :puckBottom @14:50:39.56 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @14.3psi @14:50:39.58 Polling Can every 2:00 @14:50:40.15 PuckHeater.configure :default #ambient 22.6C @14:50:40.24 PuckHeater.seek 70.4C @14:51:58.01 PuckHeater.hold 2:00,70.4C @14:53:57.71 Polling Can every 2:00 @14:53:58.27 PuckHeater.configure :default #ambient 22.6C @14:53:58.36 PuckHeater.seek 69.3C @14:53:58.55 PuckHeater.hold 2:00,69.3C @14:55:58.31 Polling Can every 2:00 @14:55:58.88 PuckHeater.hold 18:00,68.3C @15:13:55.24 Polling Can every 2:00 @15:13:55.79 PuckHeater.configure :default #ambient 22.6C @15:13:55.88 PuckHeater.seek 104.3C @15:15:00.82 PuckHeater.hold 2:00,104.3C @15:17:00.42 Polling Can every 2:00 @15:17:00.98 PuckHeater.configure :default #ambient 22.7C @15:17:01.05 PuckHeater.seek 103.2C @15:17:01.27 PuckHeater.hold 2:00,103.2C @15:19:00.90 Polling Can every 2:00 @15:19:01.45 PuckHeater.configure :default #ambient 22.7C @15:19:01.54 PuckHeater.hold 2:00,102.2C @15:21:01.25 delay 5:00 @15:26:01.34 Lysate cooked @17.7psi @15:26:01.36 Plunge.seek :puckTop @15:26:02.30 Air syringe pumping up to 5 strokes to reach 40psi @15:26:02.32 Air stroke #1 of 5 @15:26:02.46 Air.in :pressurize @15:26:02.55 Air.seek :pressurized @15:26:08.90 17.4psi after pressurize @15:26:08.94 Air.in :compress @15:26:09.04 Air.seek :evac @15:26:14.01 Reached 41.3 of 40.0psi after pumping 1659uL (0.92 strokes) @15:26:14.03 Air.in :default @15:26:14.12 Air.hold @15:26:14.23 delay 2 seconds @15:26:16.26 Air.in :fast @15:26:16.33 Air.seek :full @15:26:22.48 Plunge.seek :puckBottom @15:26:23.48 Lysis1.seek 138uL @15:26:30.06 delay 2 seconds @15:26:32.09 Plunge.seek :puckTop @15:26:33.00 Lysis1.seek :empty @15:26:39.58 Plunge.seek :puckBottom @15:26:40.64 Lysis1.in :extract @15:26:40.79 Lysis1.seek 675uL,nil @15:26:43.29 Air.in :pressurize @15:26:43.37 Air.seek :pressurized @15:26:43.65 Syringe::LysatePlungerStuck in nadia -- at < 274uL during Lysis Syringe #1 (LyS1) move @15:26:43.72 Lysis1.in :default @15:26:43.83 Lysis1.seek 244uL @15:26:49.73 14.8psi after pressurize @15:26:49.75 Air.in :compressLysate @15:26:49.88 Air.seek :evac @15:26:55.62 Reached 15.6 of 15.5psi after pumping 1467uL (0.81 strokes) @15:26:55.64 Air.in :default @15:26:55.77 Air.hold @15:26:55.91 Lysis1.in :extract @15:26:56.05 Lysis1.seek 675uL,nil @15:26:57.30 Air.in :compressLysate @15:26:57.39 Air.seek :evac @15:27:04.32 Reached 15.6 of 15.5psi after pumping 1563uL (0.86 strokes) @15:27:04.33 Air.in :default @15:27:04.42 Air.hold @15:27:05.47 Air.in :compressLysate @15:27:05.57 Air.seek :evac @15:27:09.62 Reached 15.7 of 15.5psi after pumping 1617uL (0.89 strokes) @15:27:09.64 Air.in :default @15:27:09.76 Air.hold @15:27:10.78 Air.in :compressLysate @15:27:10.89 Air.seek :evac @15:27:11.22 Syringe::LysatePlungerStuck in nadia -- at < 526uL during Lysis Syringe #1 (LyS1) move @15:27:11.28 Lysis1.in :default @15:27:11.38 Lysis1.seek 496uL @15:27:12.08 Reached 16.2 of 15.5psi after pumping 1631uL (0.90 strokes) @15:27:12.10 Air.in :default @15:27:12.20 Air.hold @15:27:12.49 Lysis1.in :extract @15:27:12.58 Lysis1.seek 675uL,nil @15:27:14.16 Air.in :compressLysate @15:27:14.24 Air.seek :evac @15:27:19.44 Air.in :default @15:27:19.52 Air.hold @15:27:19.79 Pulled 675uL lysate @15.5psi @15:27:20.00 Lysis1.seek 800uL,nil @15:27:21.41 Air.in :compressLysate @15:27:21.50 Air.seek :evac @15:27:29.14 13.1psi after compress @15:27:39.74 13.6psi after compress (stable) @15:27:45.01 Air.in :default @15:27:45.16 Air.hold @15:27:45.27 Lysis1.in :default @15:27:45.38 Lysis1.hold @15:27:45.62 Air.in :glide @15:27:45.71 Air.seek :fullVent @15:27:46.95 Handoff.select :waste @15:27:47.09 Recovered 753uL lysate @13.7psi @15:27:47.12 delay 3 seconds @15:27:50.21 After 3 delay Recovered 753uL lysate @-0.4psi @15:27:50.23 delay 5 seconds @15:27:55.28 Gate.power :heat,:OFF @15:27:55.31 Gate.power :cartridge,:OFF @15:27:55.43 PinPort.in :unlocking @15:27:55.52 PinPort.seek :clear @15:27:56.02 Archive.in :unlocking @15:27:56.15 Archive.seek :clear @15:27:56.28 Air.in :unlocking @15:27:56.45 Air.seek :clear @15:27:56.51 Lysis2.in :unlocking @15:27:56.69 Lysis2.seek :clear @15:28:01.45 Lysis1.in :unlocking @15:28:01.63 Lysis1.seek :clear @15:28:01.74 Plunge.in :unlocking @15:28:01.82 Plunge.seek :clear @15:30:54.69 -> cleanPortable @15:30:55.16 Waiting for nadia to answer "LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue" @15:31:49.36 Answered LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue"" @15:31:49.47 Lysis1.in :fast @15:31:49.55 Lysis1.seek :locked @15:31:52.11 Plunge.in :default @15:31:52.27 Plunge.seek :locked @15:31:52.40 Air.in :fast @15:31:52.48 Air.seek :locked @15:31:54.20 Powering cartridge bus @15:31:56.87 Gate.power :cartridge,:OFF @15:31:57.45 Mounted archive Cartridge @15:31:57.51 Toroid.seek :bypassed @15:31:57.61 Priming sample loop w/10ml, bypass w/10ml @15:31:57.63 SamplePump.setPosition! 0ml @15:31:57.73 Bypass.open @15:31:59.40 Intake.open @15:32:00.38 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:32:03.00 Vacuum tare is 53% of full scale at 23C (drifted 1%) @15:32:03.19 Abs.in :default @15:32:03.27 Abs.hold @15:32:03.53 SamplePump.in :start @15:32:03.61 SamplePump.seek 10ml,1:10 @15:32:04.22 SamplePump.configure :prime @15:32:15.87 Abs.stop @15:32:15.95 SamplePump.setPosition! 0ml @15:32:16.03 Exhaust.open @15:32:17.59 Bypass.close @15:32:19.32 Abs.in :default @15:32:19.42 Abs.hold @15:32:19.75 SamplePump.in :start @15:32:19.84 SamplePump.seek 10ml,1:10 @15:32:20.44 SamplePump.configure :prime @15:32:32.10 Abs.stop @15:32:32.20 Waiting for nadia to answer "INTAKE line plumbed to 10% Bleach, push return to continue" @15:42:37.82 Answered INTAKE line plumbed to 10% Bleach, push return to continue"" @15:42:37.85 Toroid.seek :bypassed @15:42:37.98 Priming sample loop w/10ml, bypass w/10ml @15:42:38.00 SamplePump.setPosition! 0ml @15:42:38.09 Exhaust.close @15:42:38.95 Bypass.open @15:42:40.82 Abs.in :default @15:42:40.89 Abs.hold @15:42:41.16 SamplePump.in :start @15:42:41.26 SamplePump.seek 10ml,1:10 @15:42:41.85 SamplePump.configure :prime @15:42:54.12 Abs.stop @15:42:54.22 SamplePump.setPosition! 0ml @15:42:54.31 Exhaust.open @15:42:55.88 Bypass.close @15:42:57.57 Abs.in :default @15:42:57.66 Abs.hold @15:42:58.00 SamplePump.in :start @15:42:58.10 SamplePump.seek 10ml,1:10 @15:42:58.70 SamplePump.configure :prime @15:43:10.36 Abs.stop @15:43:10.45 Toroid.seek :cartridge @15:43:14.81 Sampling 25.0ml @15:43:14.82 SamplePump.setPosition! 0ml @15:43:15.10 Abs.in :default @15:43:15.19 Abs.hold @15:43:15.53 SamplePump.in :start @15:43:15.62 SamplePump.seek 25ml,2:10 @15:43:16.22 SamplePump.configure :sample @15:43:45.64 Abs.stop @15:43:45.72 Intake.close @15:43:45.81 Exhaust.close @15:43:45.91 Handoff.select :close @15:43:46.01 Toroid.seek :bypassed @15:43:50.45 Sampled 25.0ml @15:43:50.47 delay 10:00 @15:53:50.54 Waiting for nadia to answer "Move INTAKE line to MillQ water" @15:53:50.58 Answered Move INTAKE line to MillQ water"" @15:53:50.61 Toroid.seek :bypassed @15:53:50.79 Priming sample loop w/50ml, bypass w/25ml @15:53:50.80 SamplePump.setPosition! 0ml @15:53:50.90 Bypass.open @15:53:52.74 Intake.open @15:53:54.43 Abs.in :default @15:53:54.52 Abs.hold @15:53:54.81 SamplePump.in :start @15:53:54.91 SamplePump.seek 25ml,2:10 @15:53:55.50 SamplePump.configure :prime @15:54:24.90 Abs.stop @15:54:24.97 SamplePump.setPosition! 0ml @15:54:25.07 Exhaust.open @15:54:26.60 Bypass.close @15:54:28.32 Abs.in :default @15:54:28.39 Abs.hold @15:54:29.38 SamplePump.in :start @15:54:29.48 SamplePump.seek 50ml,3:50 @15:54:30.07 SamplePump.configure :prime @15:55:29.04 Abs.stop @15:55:29.14 Toroid.seek :cartridge @15:55:33.50 Sampling 25.0ml @15:55:33.52 SamplePump.setPosition! 0ml @15:55:33.81 Abs.in :default @15:55:33.88 Abs.hold @15:55:34.24 SamplePump.in :start @15:55:34.33 SamplePump.seek 25ml,2:35 @15:55:34.93 SamplePump.configure :sample @15:56:04.33 Abs.stop @15:56:04.41 Intake.close @15:56:04.53 Exhaust.close @15:56:04.59 Toroid.seek :bypassed @15:56:09.07 Sampled 25.0ml @15:56:09.34 Air.in :unlocking @15:56:09.51 Air.seek :clear @15:56:10.52 Lysis1.in :unlocking @15:56:10.66 Lysis1.seek :clear @15:56:10.83 Plunge.in :unlocking @15:56:10.92 Plunge.seek :clear @15:56:12.01 Portable sample Path clean, ready for next sample @02:42:23.09PDT08-Sep-25 -> Cart.fire @02:42:23.23 Lysis1.in :fast @02:42:23.32 Lysis1.seek :locked @02:42:25.89 Plunge.in :default @02:42:25.96 Plunge.seek :locked @02:42:26.16 Air.in :fast @02:42:26.25 Air.seek :locked @02:42:27.24 Powering cartridge bus @02:42:29.92 PuckHeater.configure :default #ambient 22.7C @02:42:30.03 Gate.power :heat,:ON,limit to 5000mA @02:42:30.78 Mounted lysis Cartridge board#J59992 @02:42:30.83 Using default cartridge heat loss curve for HeatingFunctionInStage @02:42:31.11 Air.in :default @02:42:31.17 Toroid.seek :bypassed @02:42:31.27 Air.seek :sealed @02:42:31.44 Priming sample loop w/25ml, bypass w/10ml @02:42:31.47 SamplePump.setPosition! 0ml @02:42:31.55 Bypass.open @02:42:33.24 Intake.open @02:42:34.22 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @02:42:36.83 Vacuum tare is 52% of full scale at 22C (drifted -0%) @02:42:37.02 Abs.in :default @02:42:37.10 Abs.hold @02:42:37.37 SamplePump.in :start @02:42:37.46 SamplePump.seek 10ml,1:10 @02:42:38.06 SamplePump.configure :prime @02:42:49.73 Abs.stop @02:42:49.81 SamplePump.setPosition! 0ml @02:42:49.88 Exhaust.open @02:42:51.49 Bypass.close @02:42:53.85 Abs.in :default @02:42:53.94 Abs.hold @02:42:54.29 SamplePump.in :start @02:42:54.38 SamplePump.seek 25ml,2:10 @02:42:54.98 SamplePump.configure :prime @02:43:24.41 Abs.stop @02:43:24.51 Bypass.open @02:43:25.41 Intake.close @02:43:26.26 Toroid.seek :crack @02:43:30.57 Toroid.seek :cartridge @02:43:31.69 Pressurizing cartridge to 26psi above 13.7psia @02:43:31.71 SamplePump.setPosition! 0ml @02:43:31.81 Intake.open @02:43:32.71 Bypass.close @02:43:33.60 Exhaust.close @02:43:34.60 Abs.in :default @02:43:34.69 Abs.hold @02:43:34.96 SamplePump.in :charge @02:43:35.06 SamplePump.seek 2.3ml,40 seconds @02:43:51.49 Abs.stop @02:43:51.59 Intake.close @02:43:52.58 Pressurized cartridge with 1.246ml to 26.1psi @02:43:52.60 Isolated @41.2psia (27.5psi) @02:43:52.65 Verifying cartridge holds at least 0.65..0.85 of 41.2psia @02:43:52.75 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @02:43:57.36 Cartridge pressure fell 3% to 39.9psia @02:43:57.38 Toroid.seek :cartridge @02:43:57.53 Sampling 1000.0ml @02:43:57.55 SamplePump.setPosition! 0ml @02:43:57.65 Exhaust.open @02:43:58.49 Intake.open @02:43:59.54 Abs.in :default @02:43:59.62 Abs.hold @02:44:00.59 SamplePump.in :start @02:44:00.72 SamplePump.seek 1000ml,1:07:10 @02:44:01.28 SamplePump.configure :sample @02:44:03.69 filtering 2/1000ml at 0.84ml/s with 21.3psi vacuum @02:45:03.74 filtering 52/1000ml at 0.83ml/s with 22.3psi vacuum @02:46:03.74 filtering 100/1000ml at 0.80ml/s with 22.8psi vacuum @02:47:03.74 filtering 145/1000ml at 0.77ml/s with 26.1psi vacuum @02:48:03.74 filtering 190/1000ml at 0.74ml/s with 22.4psi vacuum @02:49:03.73 filtering 232/1000ml at 0.71ml/s with 22.0psi vacuum @02:50:03.78 filtering 273/1000ml at 0.68ml/s with 22.5psi vacuum @02:51:03.74 filtering 312/1000ml at 0.65ml/s with 27.9psi vacuum @02:52:03.73 filtering 350/1000ml at 0.63ml/s with 19.9psi vacuum @02:53:03.74 filtering 386/1000ml at 0.61ml/s with 24.6psi vacuum @02:54:03.75 filtering 421/1000ml at 0.58ml/s with 18.3psi vacuum @02:55:03.74 filtering 455/1000ml at 0.56ml/s with 25.1psi vacuum @02:56:03.74 filtering 488/1000ml at 0.55ml/s with 21.9psi vacuum @02:57:03.74 filtering 520/1000ml at 0.53ml/s with 28.1psi vacuum @02:58:03.75 filtering 551/1000ml at 0.52ml/s with 21.9psi vacuum @02:59:03.74 filtering 581/1000ml at 0.50ml/s with 20.4psi vacuum @03:00:03.74 filtering 610/1000ml at 0.48ml/s with 27.0psi vacuum @03:01:03.74 filtering 638/1000ml at 0.47ml/s with 21.2psi vacuum @03:02:03.74 filtering 665/1000ml at 0.46ml/s with 25.1psi vacuum @03:03:03.74 filtering 692/1000ml at 0.45ml/s with 17.1psi vacuum @03:04:03.74 filtering 719/1000ml at 0.44ml/s with 26.6psi vacuum @03:05:03.75 filtering 745/1000ml at 0.43ml/s with 27.2psi vacuum @03:06:03.73 filtering 770/1000ml at 0.43ml/s with 19.5psi vacuum @03:07:03.74 filtering 795/1000ml at 0.41ml/s with 22.7psi vacuum @03:08:03.74 filtering 819/1000ml at 0.40ml/s with 19.8psi vacuum @03:09:03.74 filtering 842/1000ml at 0.39ml/s with 21.1psi vacuum @03:10:03.75 filtering 865/1000ml at 0.38ml/s with 22.1psi vacuum @03:11:03.73 filtering 888/1000ml at 0.38ml/s with 26.1psi vacuum @03:12:03.73 filtering 911/1000ml at 0.37ml/s with 23.8psi vacuum @03:13:03.74 filtering 933/1000ml at 0.37ml/s with 21.8psi vacuum @03:14:03.74 filtering 954/1000ml at 0.36ml/s with 22.2psi vacuum @03:15:03.73 filtering 976/1000ml at 0.35ml/s with 25.1psi vacuum @03:16:03.74 filtering 996/1000ml at 0.35ml/s with 24.0psi vacuum @03:16:14.74 Abs.stop @03:16:14.93 Intake.close @03:16:14.99 Exhaust.close @03:16:15.14 Toroid.seek :bypassed @03:16:19.56 Sampled 1000.0ml @03:16:19.62 Toroid.seek :bypassed @03:16:19.74 delay 5 seconds @03:16:24.76 Handoff.select :waste @03:16:24.94 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:16:31.56 Syringe tare is 51% of full scale at 22C (drifted 0%) @03:16:31.60 PinPort.in :locking @03:16:31.69 PinPort.seek :locked @03:16:32.12 Archive.in :fast @03:16:32.19 Lysis2.in :fast @03:16:32.28 Lysis1.seek 1250uL @03:16:32.42 Archive.seek :empty @03:16:32.60 Lysis2.seek :empty @03:16:40.98 Evacuating puck with filter bubble point of 50psi @03:16:41.02 PinPort.seek :locked @03:16:41.15 Handoff.select :waste @03:16:41.18 Plunge.seek :puckBottom @03:16:42.60 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:16:49.20 Air syringe pumping up to 1 strokes to reach 8psi @03:16:49.21 Air stroke #1 of 1 @03:16:49.37 Air.in :pressurize @03:16:49.46 Air.seek :pressurized @03:16:54.81 0.1psi after pressurize @03:16:54.84 Air.in :compress @03:16:54.94 Air.seek :evac @03:17:02.56 0.7psi after compress @03:17:16.16 0.0psi after compress (stable) @03:17:16.18 Air.in :default @03:17:16.29 Air.hold @03:17:16.53 at -0.1psi Opened to waste @03:17:16.55 Handoff.select :blocked @03:17:16.72 Plunge.seek :puckTop @03:17:17.65 Air syringe pumping up to 8 strokes to reach 50psi @03:17:17.66 Air stroke #1 of 8 @03:17:17.79 Air.in :compress @03:17:17.88 Air.seek :evac @03:17:18.05 0.2psi after compress @03:17:28.79 0.3psi after compress (stable) @03:17:28.84 Air.in :gulp @03:17:28.94 Air.seek :topVent @03:17:35.60 0.3psi after gulp @03:17:41.20 0.4psi after gulp (stable) @03:17:41.21 Air stroke #2 of 8 @03:17:41.35 Air.in :pressurize @03:17:41.44 Air.seek :pressurized @03:17:47.79 0.5psi after pressurize @03:17:47.83 Air.in :compress @03:17:47.93 Air.seek :evac @03:17:55.52 15.3psi after compress @03:18:06.61 17.2psi after compress (stable) @03:18:07.20 Air.in :gulp @03:18:07.42 Air.seek :topVent @03:18:14.06 17.4psi after gulp @03:18:20.15 17.2psi after gulp (stable) @03:18:20.16 Air stroke #3 of 8 @03:18:20.32 Air.in :pressurize @03:18:20.41 Air.seek :pressurized @03:18:26.79 17.3psi after pressurize @03:18:26.82 Air.in :compress @03:18:26.92 Air.seek :evac @03:18:34.52 30.6psi after compress @03:18:45.61 32.6psi after compress (stable) @03:18:45.64 Air.in :gulp @03:18:45.75 Air.seek :topVent @03:18:52.39 32.7psi after gulp @03:18:57.98 32.5psi after gulp (stable) @03:18:58.07 Air stroke #4 of 8 @03:18:58.23 Air.in :pressurize @03:18:58.31 Air.seek :pressurized @03:19:04.70 32.5psi after pressurize @03:19:04.74 Air.in :compress @03:19:04.84 Air.seek :evac @03:19:12.44 44.5psi after compress @03:19:23.54 46.6psi after compress (stable) @03:19:23.57 Air.in :gulp @03:19:23.68 Air.seek :topVent @03:19:30.32 46.9psi after gulp @03:19:36.41 46.6psi after gulp (stable) @03:19:36.52 Air stroke #5 of 8 @03:19:36.68 Air.in :pressurize @03:19:36.78 Air.seek :pressurized @03:19:43.15 46.6psi after pressurize @03:19:43.18 Air.in :compress @03:19:43.29 Air.seek :evac @03:19:49.33 Reached 50.6 of 50.0psi after pumping 8962uL (4.95 strokes) @03:19:49.36 Air.in :default @03:19:49.47 Air.hold @03:19:49.59 Plunge.seek :puckBottom @03:19:50.50 delay 3 seconds @03:19:53.52 Handoff.select :waste @03:19:53.68 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:20:05.77 delay 1:00 @03:21:05.81 Handoff.select :blocked @03:21:05.98 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @03:21:48.20 Pressure too High, open to waste again (8.1psi) @03:21:48.22 Handoff.select :waste @03:21:48.41 delay 1:00 @03:22:48.44 Handoff.select :blocked @03:22:48.60 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @03:23:11.20 Handoff.select :waste @03:23:11.36 2.0psi at evacEnd @03:23:11.38 Handoff.select :blocked @03:23:11.56 Plunge.seek :puckTop @03:23:12.47 Air.in :fast @03:23:12.56 Air.seek :topVent @03:23:18.93 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @03:23:25.52 Stable @16.6psi after Handoff blocked @03:23:25.55 Air syringe pumping up to 8 strokes to reach 35psi @03:23:25.58 Air stroke #1 of 8 @03:23:25.73 Air.in :pressurize @03:23:25.82 Air.seek :pressurized @03:23:32.18 16.7psi after pressurize @03:23:32.21 Air.in :compress @03:23:32.32 Air.seek :evac @03:23:39.92 29.2psi after compress @03:23:50.53 31.1psi after compress (stable) @03:23:50.56 Air.in :gulp @03:23:50.73 Air.seek :topVent @03:23:57.37 31.1psi after gulp @03:24:02.99 30.9psi after gulp (stable) @03:24:03.00 Air stroke #2 of 8 @03:24:03.14 Air.in :pressurize @03:24:03.23 Air.seek :pressurized @03:24:09.57 30.8psi after pressurize @03:24:09.61 Air.in :compress @03:24:09.73 Air.seek :evac @03:24:15.50 Reached 35.5 of 35.0psi after pumping 3516uL (1.94 strokes) @03:24:15.53 Air.in :default @03:24:15.65 Air.hold @03:24:15.75 Plunge.seek :puckBottom @03:24:16.67 delay 3 seconds @03:24:19.70 Handoff.select :waste @03:24:19.88 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:24:27.12 delay 1:00 @03:25:27.17 Handoff.select :blocked @03:25:27.32 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @03:25:49.91 Handoff.select :waste @03:25:50.06 2.6psi at evacEnd @03:25:50.09 Plunge.seek :pTop @03:25:51.01 delay 5 seconds @03:25:56.04 Air.in :glide @03:25:56.12 Air.seek :fullVent @03:25:58.65 delay 2 seconds @03:26:00.70 Plunge.seek :pBottom @03:26:02.23 Seawater evacuated, Pressure above/below filter released. @03:26:02.27 Plunge.seek :locked @03:26:03.68 Handoff.select :waste @03:26:03.81 Lysis1.in :slow @03:26:03.90 Lysis1.seek 950uL @03:26:17.98 delay 5 seconds @03:26:23.00 Handoff.select :blocked @03:26:23.19 Plunge.seek :puckTop @03:26:24.23 Lysis1.seek 350uL @03:26:52.21 delay 3 seconds @03:26:55.32 Air.in mix with 5.31227 parts Lysis1 @03:26:56.07 Air.seek :evac @03:26:56.17 Lysis1.seek 190uL @03:27:03.84 Air.in :fast @03:27:03.94 Air.seek :full @03:27:10.64 Lysis1.seek :empty @03:27:19.79 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @11.5psi @03:27:19.81 Plunge.seek :puckBottom @03:27:20.87 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @10.0psi @03:27:20.89 Polling Can every 2:00 @03:27:21.47 PuckHeater.configure :default #ambient 22.1C @03:27:21.55 PuckHeater.seek 70.4C @03:28:32.97 PuckHeater.hold 2:00,70.4C @03:30:32.83 Polling Can every 2:00 @03:30:33.38 PuckHeater.configure :default #ambient 22.1C @03:30:33.46 PuckHeater.seek 69.4C @03:30:33.68 PuckHeater.hold 2:00,69.4C @03:32:33.46 Polling Can every 2:00 @03:32:34.01 PuckHeater.configure :default #ambient 22.1C @03:32:34.10 PuckHeater.hold 18:00,68.3C @03:50:31.06 Polling Can every 2:00 @03:50:31.62 PuckHeater.configure :default #ambient 22.1C @03:50:31.69 PuckHeater.seek 104.3C @03:51:29.26 PuckHeater.hold 2:00,104.3C @03:53:28.99 Polling Can every 2:00 @03:53:29.54 PuckHeater.configure :default #ambient 22.2C @03:53:29.63 PuckHeater.seek 103.3C @03:53:29.86 PuckHeater.hold 2:00,103.3C @03:55:29.64 Polling Can every 2:00 @03:55:30.20 PuckHeater.hold 2:00,102.2C @03:57:30.02 delay 5:00 @04:02:30.10 Lysate cooked @6.3psi @04:02:30.13 Plunge.seek :puckTop @04:02:31.05 Air syringe pumping up to 5 strokes to reach 40psi @04:02:31.06 Air stroke #1 of 5 @04:02:31.20 Air.in :pressurize @04:02:31.27 Air.seek :pressurized @04:02:37.63 5.8psi after pressurize @04:02:37.66 Air.in :compress @04:02:37.77 Air.seek :evac @04:02:43.72 Reached 41.2 of 40.0psi after pumping 1715uL (0.95 strokes) @04:02:43.74 Air.in :default @04:02:43.84 Air.hold @04:02:44.56 delay 2 seconds @04:02:46.63 Air.in :fast @04:02:46.76 Air.seek :full @04:02:53.11 Plunge.seek :puckBottom @04:02:54.10 Lysis1.seek 138uL @04:03:00.72 delay 2 seconds @04:03:02.75 Plunge.seek :puckTop @04:03:03.66 Lysis1.seek :empty @04:03:10.24 Plunge.seek :puckBottom @04:03:11.32 Lysis1.in :extract @04:03:11.43 Lysis1.seek 675uL,nil @04:03:13.78 Air.in :pressurize @04:03:13.88 Air.seek :pressurized @04:03:20.30 11.7psi after pressurize @04:03:20.33 Air.in :compressLysate @04:03:20.46 Air.seek :evac @04:03:50.88 13.9psi after compress @04:03:51.21 Air.in :default @04:03:51.28 Air.hold @04:03:51.51 Pulled 675uL lysate @12.3psi @04:03:52.37 Lysis1.seek 800uL,nil @04:03:52.79 Air.in :compressLysate @04:03:52.92 Air.seek :evac @04:03:53.16 12.6psi after compress @04:04:03.90 13.1psi after compress (stable) @04:04:09.20 Air.in :default @04:04:09.27 Air.hold @04:04:09.37 Lysis1.in :default @04:04:09.46 Lysis1.hold @04:04:09.72 Air.in :glide @04:04:09.79 Air.seek :fullVent @04:04:11.04 Handoff.select :waste @04:04:11.19 Recovered 675uL lysate @13.1psi @04:04:11.21 delay 3 seconds @04:04:14.31 After 3 delay Recovered 675uL lysate @-1.3psi @04:04:14.33 delay 5 seconds @04:04:19.38 Gate.power :heat,:OFF @04:04:19.40 Gate.power :cartridge,:OFF @04:04:19.54 PinPort.in :unlocking @04:04:19.62 PinPort.seek :clear @04:04:20.12 Archive.in :unlocking @04:04:20.29 Archive.seek :clear @04:04:20.38 Air.in :unlocking @04:04:20.59 Air.seek :clear @04:04:20.73 Lysis2.in :unlocking @04:04:20.84 Lysis2.seek :clear @04:04:25.59 Lysis1.in :unlocking @04:04:25.74 Lysis1.seek :clear @04:04:25.82 Plunge.in :unlocking @04:04:25.94 Plunge.seek :clear @04:16:32.12 -> cleanPortable @04:16:32.58 Waiting for nadia to answer "LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue" @04:16:35.06 Answered LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue"" @04:16:35.17 Lysis1.in :fast @04:16:35.25 Lysis1.seek :locked @04:16:37.81 Plunge.in :default @04:16:37.88 Plunge.seek :locked @04:16:38.13 Air.in :fast @04:16:38.20 Air.seek :locked @04:16:39.20 Powering cartridge bus @04:16:41.87 Gate.power :cartridge,:OFF @04:16:42.46 Mounted archive Cartridge @04:16:42.52 Toroid.seek :bypassed @04:16:42.62 Priming sample loop w/10ml, bypass w/10ml @04:16:42.64 SamplePump.setPosition! 0ml @04:16:42.77 Bypass.open @04:16:44.45 Intake.open @04:16:45.44 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @04:16:48.07 Vacuum tare is 52% of full scale at 22C (drifted 0%) @04:16:48.31 Abs.in :default @04:16:48.39 Abs.hold @04:16:48.67 SamplePump.in :start @04:16:48.80 SamplePump.seek 10ml,1:10 @04:16:49.35 SamplePump.configure :prime @04:17:01.07 Abs.stop @04:17:01.14 SamplePump.setPosition! 0ml @04:17:01.22 Exhaust.open @04:17:02.79 Bypass.close @04:17:04.49 Abs.in :default @04:17:04.56 Abs.hold @04:17:04.93 SamplePump.in :start @04:17:05.03 SamplePump.seek 10ml,1:10 @04:17:05.62 SamplePump.configure :prime @04:17:17.31 Abs.stop @04:17:17.39 Waiting for nadia to answer "INTAKE line plumbed to 10% Bleach, push return to continue" @04:17:26.57 Answered INTAKE line plumbed to 10% Bleach, push return to continue"" @04:17:26.59 Toroid.seek :bypassed @04:17:27.39 Priming sample loop w/10ml, bypass w/10ml @04:17:27.42 SamplePump.setPosition! 0ml @04:17:27.51 Exhaust.close @04:17:28.34 Bypass.open @04:17:30.15 Abs.in :default @04:17:30.23 Abs.hold @04:17:30.50 SamplePump.in :start @04:17:30.59 SamplePump.seek 10ml,1:10 @04:17:31.19 SamplePump.configure :prime @04:17:42.89 Abs.stop @04:17:42.97 SamplePump.setPosition! 0ml @04:17:43.04 Exhaust.open @04:17:44.59 Bypass.close @04:17:46.33 Abs.in :default @04:17:46.41 Abs.hold @04:17:46.77 SamplePump.in :start @04:17:46.87 SamplePump.seek 10ml,1:10 @04:17:47.46 SamplePump.configure :prime @04:17:59.15 Abs.stop @04:17:59.23 Toroid.seek :cartridge @04:18:03.59 Sampling 25.0ml @04:18:03.61 SamplePump.setPosition! 0ml @04:18:03.87 Abs.in :default @04:18:03.94 Abs.hold @04:18:04.29 SamplePump.in :start @04:18:04.38 SamplePump.seek 25ml,2:10 @04:18:04.98 SamplePump.configure :sample @04:18:34.42 Abs.stop @04:18:34.50 Intake.close @04:18:34.58 Exhaust.close @04:18:34.75 Handoff.select :close @04:18:34.84 Toroid.seek :bypassed @04:18:39.25 Sampled 25.0ml @04:18:39.27 delay 10:00 @04:28:39.33 Waiting for nadia to answer "Move INTAKE line to MillQ water" @04:28:39.37 Answered Move INTAKE line to MillQ water"" @04:28:39.39 Toroid.seek :bypassed @04:28:39.52 Priming sample loop w/50ml, bypass w/25ml @04:28:39.54 SamplePump.setPosition! 0ml @04:28:39.64 Bypass.open @04:28:41.38 Intake.open @04:28:43.07 Abs.in :default @04:28:43.14 Abs.hold @04:28:44.03 SamplePump.in :start @04:28:44.12 SamplePump.seek 25ml,2:10 @04:28:44.72 SamplePump.configure :prime @04:29:14.17 Abs.stop @04:29:14.25 SamplePump.setPosition! 0ml @04:29:14.35 Exhaust.open @04:29:15.90 Bypass.close @04:29:17.62 Abs.in :default @04:29:17.71 Abs.hold @04:29:18.10 SamplePump.in :start @04:29:18.19 SamplePump.seek 50ml,3:50 @04:29:18.79 SamplePump.configure :prime @04:30:17.86 Abs.stop @04:30:17.95 Toroid.seek :cartridge @04:30:22.32 Sampling 25.0ml @04:30:22.34 SamplePump.setPosition! 0ml @04:30:22.62 Abs.in :default @04:30:22.73 Abs.hold @04:30:23.09 SamplePump.in :start @04:30:23.18 SamplePump.seek 25ml,2:35 @04:30:23.78 SamplePump.configure :sample @04:30:53.24 Abs.stop @04:30:53.33 Intake.close @04:30:53.39 Exhaust.close @04:30:53.47 Toroid.seek :bypassed @04:30:57.93 Sampled 25.0ml @04:30:58.22 Air.in :unlocking @04:30:58.39 Air.seek :clear @04:30:59.39 Lysis1.in :unlocking @04:30:59.56 Lysis1.seek :clear @04:30:59.65 Plunge.in :unlocking @04:30:59.76 Plunge.seek :clear @04:31:00.91 Portable sample Path clean, ready for next sample @14:36:09.64 -> Log.comment{Ano Sample, 5um} @14:36:09.68 SyntaxError in nadia -- compile error nadia:26: syntax error, unexpected tIDENTIFIER, expecting '}' Log.comment{Ano Sample, 5um} ^ @14:36:20.25 -> Log.comment "Ano Sample, 5um" @14:36:20.27 Ano Sample, 5um @14:36:36.02 -> Log.comment "Surface sample" @14:36:36.04 Surface sample @14:37:06.05 -> BACsampler.goalVolume=5L @14:37:06.09 SyntaxError in nadia -- compile error nadia:29: syntax error, unexpected tCONSTANT, expecting $end BACsampler.goalVolume=5L ^ @14:37:10.94 -> BACsampler.goalVolume=5000 5000 @14:37:18.61 -> BACsampler.goalVolume 5000 @14:37:27.95 -> ESP.reready! @14:37:27.99 Plunge.in :default @14:37:28.07 Toroid.in :default @14:37:28.14 PinPort.in :default @14:37:28.35 Archive.in :default @14:37:28.44 Air.in :default @14:37:28.48 Toroid.home.jog 0 @14:37:28.53 Lysis1.in :default @14:37:28.58 Lysis2.in :default @14:37:28.65 Plunge.home.jog 0 @14:37:28.75 PinPort.home.jog 0 @14:37:28.93 Archive.home.jog 0 @14:37:29.10 Air.home.jog 0 @14:37:29.20 Lysis1.home.jog 0 @14:37:29.26 Lysis2.home.jog 0 @14:37:29.96 Plunge.in :homing @14:37:30.11 Toroid.in :homing @14:37:30.23 Toroid.jog 261 @14:37:30.25 Plunge.jog 537 @14:37:30.54 Lysis1.in :homing @14:37:30.59 Air.in :homing @14:37:30.63 PinPort.in :homing @14:37:30.71 Lysis2.in :homing @14:37:30.77 Archive.in :homing @14:37:31.01 Lysis1.jog 1662 @14:37:31.04 Air.jog 1567 @14:37:31.10 Lysis2.jog 1614 @14:37:31.16 PinPort.jog -711 @14:37:31.22 Archive.jog 1619 @14:37:31.60 Plunge.setRawPosition! 50 @14:37:31.62 Toroid.setRawPosition! 0 @14:37:31.76 Plunge.seek :home @14:37:31.79 Toroid.seek :home @14:37:32.80 Lysis2.setRawPosition! 50 @14:37:32.86 Lysis1.setRawPosition! 50 @14:37:32.89 Archive.setRawPosition! 50 @14:37:32.95 Toroid.in :default @14:37:33.00 Air.setRawPosition! 50 @14:37:33.04 Plunge.in :default @14:37:33.08 PinPort.setRawPosition! -50 @14:37:33.35 Lysis2.seek :home @14:37:33.41 Lysis1.seek :home @14:37:33.46 Archive.seek :home @14:37:33.70 PinPort.seek :home @14:37:33.76 Air.seek :home @14:37:34.19 Lysis1.in :default @14:37:34.23 Archive.in :default @14:37:34.26 Lysis2.in :default @14:37:34.29 PinPort.in :default @14:37:34.57 Air.in :default @14:37:34.93 Toroid.seek :clear @14:37:35.13 PinPort.in :unlocking @14:37:35.22 PinPort.seek :clear @14:37:37.21 Air.seek :clear @14:37:37.89 Lysis1.seek :clear @14:37:38.80 Lysis2.seek :clear @14:37:39.61 Archive.seek :clear Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 25ml Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Abs = Absolute Pressure (Leak) Sensor @13psia Toroid = Toroid Valve (TV) clear PinPort = Handoff Pin Port (PP) clear Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear @14:37:45.90 -> Cart.fire @14:37:46.03 Lysis1.in :fast @14:37:46.11 Lysis1.seek :locked @14:37:48.69 Plunge.seek :locked @14:37:48.92 Air.in :fast @14:37:48.99 Air.seek :locked @14:37:49.99 Powering cartridge bus @14:37:52.70 PuckHeater.configure :default #ambient 22.2C @14:37:52.80 Gate.power :heat,:ON,limit to 5000mA @14:37:53.48 Mounted lysis Cartridge board#J59977 @14:37:53.53 Using default cartridge heat loss curve for HeatingFunctionInStage @14:37:53.79 Air.in :default @14:37:53.88 Air.seek :sealed @14:37:53.90 Toroid.seek :bypassed @14:37:56.20 Priming sample loop w/25ml, bypass w/10ml @14:37:56.23 SamplePump.setPosition! 0ml @14:37:56.31 Bypass.open @14:37:57.98 Intake.open @14:37:58.99 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:38:01.60 Vacuum tare is 52% of full scale at 22C (drifted 0%) @14:38:01.78 Abs.in :default @14:38:01.85 Abs.hold @14:38:02.13 SamplePump.in :start @14:38:02.23 SamplePump.seek 10ml,1:10 @14:38:02.82 SamplePump.configure :prime @14:38:14.50 Abs.stop @14:38:14.58 SamplePump.setPosition! 0ml @14:38:14.65 Exhaust.open @14:38:16.28 Bypass.close @14:38:18.04 Abs.in :default @14:38:18.11 Abs.hold @14:38:18.44 SamplePump.in :start @14:38:18.54 SamplePump.seek 25ml,2:10 @14:38:19.13 SamplePump.configure :prime @14:38:48.61 Abs.stop @14:38:48.72 Bypass.open @14:38:49.59 Intake.close @14:38:50.48 Toroid.seek :crack @14:38:54.77 Toroid.seek :cartridge @14:38:55.89 Pressurizing cartridge to 26psi above 13.6psia @14:38:55.91 SamplePump.setPosition! 0ml @14:38:56.01 Intake.open @14:38:56.89 Bypass.close @14:38:57.76 Exhaust.close @14:38:58.77 Abs.in :default @14:38:58.90 Abs.hold @14:38:59.15 SamplePump.in :charge @14:38:59.24 SamplePump.seek 2.3ml,40 seconds @14:39:15.18 Abs.stop @14:39:15.27 Intake.close @14:39:16.85 Pressurized cartridge with 1.172ml to 26.2psi @14:39:16.89 Isolated @40.8psia (27.2psi) @14:39:16.92 Verifying cartridge holds at least 0.65..0.85 of 40.8psia @14:39:17.00 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:39:21.61 Cartridge pressure fell 2% to 39.8psia @14:39:21.64 Toroid.seek :cartridge @14:39:21.77 Sampling 5000.0ml @14:39:21.79 SamplePump.setPosition! 0ml @14:39:21.88 Exhaust.open @14:39:22.73 Intake.open @14:39:23.81 Abs.in :default @14:39:23.90 Abs.hold @14:39:24.25 SamplePump.in :start @14:39:24.35 SamplePump.seek 5000ml,5:33:50 @14:39:24.94 SamplePump.configure :sample @14:39:27.33 filtering 2/5000ml at 0.84ml/s with 5.7psi vacuum @14:40:27.40 filtering 53/5000ml at 0.85ml/s with 6.5psi vacuum @14:41:27.40 filtering 104/5000ml at 0.84ml/s with 8.4psi vacuum @14:42:27.39 filtering 154/5000ml at 0.84ml/s with 6.3psi vacuum @14:43:27.39 filtering 205/5000ml at 0.84ml/s with 9.1psi vacuum @14:44:27.39 filtering 256/5000ml at 0.84ml/s with 9.5psi vacuum @14:45:27.40 filtering 306/5000ml at 0.84ml/s with 10.1psi vacuum @14:46:27.39 filtering 357/5000ml at 0.84ml/s with 11.3psi vacuum @14:47:27.40 filtering 408/5000ml at 0.84ml/s with 12.0psi vacuum @14:48:27.40 filtering 458/5000ml at 0.84ml/s with 9.4psi vacuum @14:49:27.40 filtering 509/5000ml at 0.84ml/s with 12.3psi vacuum @14:50:27.39 filtering 560/5000ml at 0.84ml/s with 11.6psi vacuum @14:51:27.39 filtering 610/5000ml at 0.84ml/s with 13.3psi vacuum @14:52:27.39 filtering 661/5000ml at 0.84ml/s with 12.4psi vacuum @14:53:27.39 filtering 712/5000ml at 0.84ml/s with 12.3psi vacuum @14:54:27.39 filtering 762/5000ml at 0.84ml/s with 11.7psi vacuum @14:55:27.40 filtering 813/5000ml at 0.84ml/s with 13.1psi vacuum @14:56:27.39 filtering 864/5000ml at 0.84ml/s with 8.7psi vacuum @14:57:27.38 filtering 914/5000ml at 0.84ml/s with 13.3psi vacuum @14:58:28.05 filtering 965/5000ml at 0.84ml/s with 15.3psi vacuum @14:59:27.39 filtering 1016/5000ml at 0.84ml/s with 13.9psi vacuum @15:00:27.39 filtering 1066/5000ml at 0.84ml/s with 15.9psi vacuum @15:01:27.40 filtering 1117/5000ml at 0.84ml/s with 14.1psi vacuum @15:02:27.39 filtering 1168/5000ml at 0.84ml/s with 16.0psi vacuum @15:03:27.39 filtering 1218/5000ml at 0.84ml/s with 11.8psi vacuum @15:04:27.38 filtering 1269/5000ml at 0.84ml/s with 15.7psi vacuum @15:05:27.39 filtering 1320/5000ml at 0.84ml/s with 16.6psi vacuum @15:06:27.40 filtering 1370/5000ml at 0.84ml/s with 16.7psi vacuum @15:07:27.39 filtering 1421/5000ml at 0.84ml/s with 19.6psi vacuum @15:08:27.39 filtering 1472/5000ml at 0.84ml/s with 13.6psi vacuum @15:09:27.39 filtering 1522/5000ml at 0.84ml/s with 19.1psi vacuum @15:10:27.38 filtering 1573/5000ml at 0.84ml/s with 17.9psi vacuum @15:11:27.39 filtering 1624/5000ml at 0.84ml/s with 12.7psi vacuum @15:12:27.39 filtering 1674/5000ml at 0.84ml/s with 16.7psi vacuum @15:13:27.40 filtering 1725/5000ml at 0.84ml/s with 15.2psi vacuum @15:14:27.40 filtering 1776/5000ml at 0.84ml/s with 16.9psi vacuum @15:15:27.39 filtering 1826/5000ml at 0.84ml/s with 16.5psi vacuum @15:16:27.39 filtering 1877/5000ml at 0.85ml/s with 17.8psi vacuum @15:17:27.40 filtering 1928/5000ml at 0.84ml/s with 17.8psi vacuum @15:18:27.39 filtering 1978/5000ml at 0.84ml/s with 22.6psi vacuum @15:19:27.39 filtering 2028/5000ml at 0.84ml/s with 23.9psi vacuum @15:20:27.39 filtering 2078/5000ml at 0.83ml/s with 19.6psi vacuum @15:21:27.39 filtering 2128/5000ml at 0.83ml/s with 20.1psi vacuum @15:22:27.39 filtering 2177/5000ml at 0.82ml/s with 18.6psi vacuum @15:23:27.38 filtering 2226/5000ml at 0.81ml/s with 17.0psi vacuum @15:24:27.39 filtering 2275/5000ml at 0.81ml/s with 21.8psi vacuum @15:25:27.39 filtering 2323/5000ml at 0.80ml/s with 14.6psi vacuum @15:26:27.39 filtering 2370/5000ml at 0.79ml/s with 24.5psi vacuum @15:27:27.39 filtering 2417/5000ml at 0.79ml/s with 14.7psi vacuum @15:28:27.39 filtering 2464/5000ml at 0.77ml/s with 26.6psi vacuum @15:29:27.39 filtering 2510/5000ml at 0.76ml/s with 17.1psi vacuum @15:30:27.38 filtering 2555/5000ml at 0.75ml/s with 23.4psi vacuum @15:31:27.38 filtering 2599/5000ml at 0.74ml/s with 22.4psi vacuum @15:32:27.39 filtering 2643/5000ml at 0.73ml/s with 26.3psi vacuum @15:33:27.38 filtering 2686/5000ml at 0.71ml/s with 26.2psi vacuum @15:34:27.92 filtering 2728/5000ml at 0.70ml/s with 19.6psi vacuum @15:35:27.39 filtering 2769/5000ml at 0.69ml/s with 24.9psi vacuum @15:36:27.39 filtering 2810/5000ml at 0.68ml/s with 15.1psi vacuum @15:37:27.39 filtering 2850/5000ml at 0.66ml/s with 27.5psi vacuum @15:38:27.40 filtering 2888/5000ml at 0.64ml/s with 15.2psi vacuum @15:39:27.40 filtering 2926/5000ml at 0.63ml/s with 29.4psi vacuum @15:40:27.40 filtering 2963/5000ml at 0.62ml/s with 23.9psi vacuum @15:41:27.39 filtering 3000/5000ml at 0.60ml/s with 29.0psi vacuum @15:42:27.39 filtering 3035/5000ml at 0.59ml/s with 15.4psi vacuum @15:43:27.40 filtering 3070/5000ml at 0.59ml/s with 23.1psi vacuum @15:44:27.39 filtering 3104/5000ml at 0.57ml/s with 23.3psi vacuum @15:45:27.40 filtering 3138/5000ml at 0.55ml/s with 22.1psi vacuum @15:46:27.39 filtering 3170/5000ml at 0.54ml/s with 24.1psi vacuum @15:47:27.40 filtering 3202/5000ml at 0.53ml/s with 25.7psi vacuum @15:48:27.39 filtering 3233/5000ml at 0.52ml/s with 21.9psi vacuum @15:49:27.39 filtering 3264/5000ml at 0.51ml/s with 27.1psi vacuum @15:50:27.38 filtering 3294/5000ml at 0.50ml/s with 15.8psi vacuum @15:51:27.38 filtering 3324/5000ml at 0.50ml/s with 24.3psi vacuum @15:52:27.39 filtering 3353/5000ml at 0.48ml/s with 21.4psi vacuum @15:53:27.40 filtering 3382/5000ml at 0.48ml/s with 29.6psi vacuum @15:54:27.38 filtering 3410/5000ml at 0.47ml/s with 22.7psi vacuum @15:55:27.38 filtering 3438/5000ml at 0.47ml/s with 30.5psi vacuum @15:56:27.39 filtering 3467/5000ml at 0.48ml/s with 19.6psi vacuum @15:57:27.40 filtering 3496/5000ml at 0.48ml/s with 28.6psi vacuum @15:58:27.39 filtering 3524/5000ml at 0.47ml/s with 28.0psi vacuum @15:59:27.39 filtering 3552/5000ml at 0.46ml/s with 22.6psi vacuum @16:00:27.39 filtering 3579/5000ml at 0.45ml/s with 21.4psi vacuum @16:01:27.39 filtering 3605/5000ml at 0.44ml/s with 26.8psi vacuum @16:02:27.39 filtering 3631/5000ml at 0.44ml/s with 21.4psi vacuum @16:03:27.39 filtering 3657/5000ml at 0.43ml/s with 23.7psi vacuum @16:04:27.38 filtering 3682/5000ml at 0.42ml/s with 28.3psi vacuum @16:05:27.39 filtering 3706/5000ml at 0.41ml/s with 22.2psi vacuum @16:06:27.38 filtering 3730/5000ml at 0.40ml/s with 19.9psi vacuum @16:07:27.39 filtering 3753/5000ml at 0.39ml/s with 22.9psi vacuum @16:08:27.39 filtering 3776/5000ml at 0.38ml/s with 24.3psi vacuum @16:09:27.39 filtering 3799/5000ml at 0.38ml/s with 22.6psi vacuum @16:10:27.93 filtering 3822/5000ml at 0.38ml/s with 18.4psi vacuum @16:11:27.40 filtering 3845/5000ml at 0.38ml/s with 22.9psi vacuum @16:12:27.40 filtering 3868/5000ml at 0.38ml/s with 22.5psi vacuum @16:13:27.40 filtering 3890/5000ml at 0.38ml/s with 23.0psi vacuum @16:14:27.41 filtering 3913/5000ml at 0.38ml/s with 23.5psi vacuum @16:15:27.39 filtering 3935/5000ml at 0.37ml/s with 27.6psi vacuum @16:16:27.39 filtering 3958/5000ml at 0.37ml/s with 24.0psi vacuum @16:17:27.39 filtering 3980/5000ml at 0.37ml/s with 16.8psi vacuum @16:18:27.39 filtering 4002/5000ml at 0.38ml/s with 26.4psi vacuum @16:19:27.40 filtering 4026/5000ml at 0.38ml/s with 22.8psi vacuum @16:20:27.40 filtering 4049/5000ml at 0.39ml/s with 23.3psi vacuum @16:21:27.40 filtering 4073/5000ml at 0.40ml/s with 22.6psi vacuum @16:22:27.38 filtering 4098/5000ml at 0.41ml/s with 12.7psi vacuum @16:23:27.40 filtering 4123/5000ml at 0.41ml/s with 25.0psi vacuum @16:24:27.39 filtering 4147/5000ml at 0.41ml/s with 22.5psi vacuum @16:25:27.40 filtering 4172/5000ml at 0.41ml/s with 33.1psi vacuum @16:26:27.40 filtering 4197/5000ml at 0.41ml/s with 22.8psi vacuum @16:27:27.44 filtering 4221/5000ml at 0.40ml/s with 16.1psi vacuum @16:28:27.39 filtering 4244/5000ml at 0.40ml/s with 22.8psi vacuum @16:29:27.39 filtering 4268/5000ml at 0.39ml/s with 24.1psi vacuum @16:30:27.40 filtering 4290/5000ml at 0.38ml/s with 25.9psi vacuum @16:31:27.44 filtering 4313/5000ml at 0.37ml/s with 22.4psi vacuum @16:32:27.39 filtering 4335/5000ml at 0.36ml/s with 21.6psi vacuum @16:33:27.40 filtering 4357/5000ml at 0.36ml/s with 23.2psi vacuum @16:34:27.39 filtering 4378/5000ml at 0.36ml/s with 21.9psi vacuum @16:35:27.40 filtering 4400/5000ml at 0.37ml/s with 22.7psi vacuum @16:36:27.38 filtering 4423/5000ml at 0.37ml/s with 22.8psi vacuum @16:37:27.40 filtering 4445/5000ml at 0.37ml/s with 23.8psi vacuum @16:38:27.39 filtering 4467/5000ml at 0.36ml/s with 22.7psi vacuum @16:39:24.16 Abs.stop @16:39:24.24 SamplePump.stop @16:39:24.44 Sea 9.0m deep (28.0psia) @16:39:24.47 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:39:27.11 Vacuum tare is 54% of full scale at 23C (drifted 3%) @16:39:27.85 Abs.hold @16:39:28.29 SamplePump.in :start @16:39:28.39 SamplePump.seek 5000ml,34:42 @16:39:28.98 SamplePump.configure :sample @16:39:31.38 filtering 4489/5000ml at 0.77ml/s with 7.1psi vacuum @16:40:31.43 filtering 4525/5000ml at 0.61ml/s with 13.6psi vacuum 4.3m deep (21.1psia) @16:41:31.43 filtering 4562/5000ml at 0.61ml/s with 26.3psi vacuum @16:42:31.43 filtering 4598/5000ml at 0.61ml/s with 26.5psi vacuum @16:43:31.43 filtering 4635/5000ml at 0.61ml/s with 8.5psi vacuum @16:44:31.44 filtering 4671/5000ml at 0.60ml/s with 10.1psi vacuum 4.1m deep (20.7psia) @16:45:31.43 filtering 4707/5000ml at 0.60ml/s with 16.6psi vacuum 4.6m deep (21.5psia) @16:46:31.43 filtering 4742/5000ml at 0.59ml/s with 22.1psi vacuum @16:47:31.43 filtering 4777/5000ml at 0.59ml/s with 25.3psi vacuum @16:48:31.42 filtering 4812/5000ml at 0.58ml/s with 24.5psi vacuum 3.9m deep (20.4psia) @16:49:31.42 filtering 4847/5000ml at 0.57ml/s with 14.1psi vacuum 3.8m deep (20.3psia) @16:50:31.43 filtering 4881/5000ml at 0.57ml/s with 18.8psi vacuum @16:51:31.43 filtering 4915/5000ml at 0.57ml/s with 22.6psi vacuum @16:52:31.43 filtering 4948/5000ml at 0.56ml/s with 23.1psi vacuum @16:53:31.43 filtering 4981/5000ml at 0.55ml/s with 19.3psi vacuum @16:54:05.60 Abs.stop @16:54:05.72 Intake.close @16:54:05.79 Exhaust.close @16:54:05.95 Toroid.seek :bypassed @16:54:10.33 Sampled 5000.0ml @16:54:10.38 Toroid.seek :bypassed @16:54:10.52 delay 5 seconds @16:54:15.56 Handoff.select :waste @16:54:15.73 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:54:22.35 Syringe tare is 52% of full scale at 23C (drifted 1%) @16:54:22.39 PinPort.in :locking @16:54:22.50 PinPort.seek :locked @16:54:23.31 Archive.in :fast @16:54:23.42 Lysis2.in :fast @16:54:23.48 Lysis1.seek 1250uL @16:54:23.60 Archive.seek :empty @16:54:23.66 Lysis2.seek :empty @16:54:32.14 Evacuating puck with filter bubble point of 50psi @16:54:32.17 PinPort.seek :locked @16:54:32.33 Handoff.select :waste @16:54:32.36 Plunge.seek :puckBottom @16:54:33.77 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:54:40.37 Air syringe pumping up to 1 strokes to reach 8psi @16:54:40.38 Air stroke #1 of 1 @16:54:40.52 Air.in :pressurize @16:54:40.59 Air.seek :pressurized @16:54:45.95 0.0psi after pressurize @16:54:45.98 Air.in :compress @16:54:46.15 Air.seek :evac @16:54:53.76 3.6psi after compress @16:55:06.35 0.2psi after compress (stable) @16:55:06.38 Air.in :default @16:55:06.48 Air.hold @16:55:06.80 at 0.2psi Opened to waste @16:55:06.82 Handoff.select :blocked @16:55:07.00 Plunge.seek :puckTop @16:55:07.92 Air syringe pumping up to 8 strokes to reach 50psi @16:55:07.93 Air stroke #1 of 8 @16:55:08.07 Air.in :compress @16:55:08.16 Air.seek :evac @16:55:08.34 2.1psi after compress @16:55:18.95 2.1psi after compress (stable) @16:55:18.98 Air.in :gulp @16:55:19.09 Air.seek :topVent @16:55:25.73 2.0psi after gulp @16:55:31.32 2.1psi after gulp (stable) @16:55:31.33 Air stroke #2 of 8 @16:55:31.48 Air.in :pressurize @16:55:31.57 Air.seek :pressurized @16:55:38.53 2.5psi after pressurize @16:55:38.60 Air.in :compress @16:55:38.75 Air.seek :evac @16:55:46.36 18.0psi after compress @16:55:56.95 19.9psi after compress (stable) @16:55:56.99 Air.in :gulp @16:55:57.11 Air.seek :topVent @16:56:03.75 19.8psi after gulp @16:56:09.84 19.8psi after gulp (stable) @16:56:09.85 Air stroke #3 of 8 @16:56:10.00 Air.in :pressurize @16:56:10.07 Air.seek :pressurized @16:56:16.40 19.5psi after pressurize @16:56:16.44 Air.in :compress @16:56:16.55 Air.seek :evac @16:56:24.15 34.0psi after compress @16:56:35.25 35.9psi after compress (stable) @16:56:35.34 Air.in :gulp @16:56:35.44 Air.seek :topVent @16:56:42.09 35.9psi after gulp @16:56:47.68 35.8psi after gulp (stable) @16:56:47.69 Air stroke #4 of 8 @16:56:47.83 Air.in :pressurize @16:56:47.92 Air.seek :pressurized @16:56:54.29 35.9psi after pressurize @16:56:54.32 Air.in :compress @16:56:54.43 Air.seek :evac @16:57:02.03 49.0psi after compress @16:57:02.26 Reached 49.9 of 50.0psi after pumping 7242uL (4.00 strokes) @16:57:02.29 Air.in :default @16:57:02.39 Air.hold @16:57:02.51 Plunge.seek :puckBottom @16:57:03.41 delay 3 seconds @16:57:06.43 Handoff.select :waste @16:57:06.60 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:57:15.20 delay 1:00 @16:58:15.24 Handoff.select :blocked @16:58:15.42 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @16:58:38.02 Handoff.select :waste @16:58:38.17 -0.2psi at evacEnd @16:58:38.19 Handoff.select :blocked @16:58:38.36 Plunge.seek :puckTop @16:58:39.27 Air.in :fast @16:58:39.36 Air.seek :topVent @16:58:46.04 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @16:58:52.63 Stable @1.1psi after Handoff blocked @16:58:52.68 Air syringe pumping up to 8 strokes to reach 35psi @16:58:52.74 Air stroke #1 of 8 @16:58:52.88 Air.in :pressurize @16:58:52.95 Air.seek :pressurized @16:58:59.32 1.1psi after pressurize @16:58:59.35 Air.in :compress @16:58:59.45 Air.seek :evac @16:59:07.06 15.0psi after compress @16:59:17.66 16.6psi after compress (stable) @16:59:17.69 Air.in :gulp @16:59:17.83 Air.seek :topVent @16:59:24.47 16.5psi after gulp @16:59:30.56 16.5psi after gulp (stable) @16:59:30.58 Air stroke #2 of 8 @16:59:30.75 Air.in :pressurize @16:59:30.83 Air.seek :pressurized @16:59:37.19 16.6psi after pressurize @16:59:37.23 Air.in :compress @16:59:37.33 Air.seek :evac @16:59:44.94 29.7psi after compress @16:59:55.54 31.4psi after compress (stable) @16:59:55.57 Air.in :gulp @16:59:55.69 Air.seek :topVent @17:00:02.33 31.4psi after gulp @17:00:08.43 31.3psi after gulp (stable) @17:00:08.52 Air stroke #3 of 8 @17:00:08.65 Air.in :pressurize @17:00:08.79 Air.seek :pressurized @17:00:15.16 31.3psi after pressurize @17:00:15.19 Air.in :compress @17:00:15.29 Air.seek :evac @17:00:20.82 Reached 35.6 of 35.0psi after pumping 5310uL (2.93 strokes) @17:00:20.84 Air.in :default @17:00:20.96 Air.hold @17:00:21.08 Plunge.seek :puckBottom @17:00:22.00 delay 3 seconds @17:00:25.02 Handoff.select :waste @17:00:25.18 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @17:00:33.28 delay 1:00 @17:01:33.31 Handoff.select :blocked @17:01:33.47 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @17:01:56.06 Handoff.select :waste @17:01:56.21 -0.1psi at evacEnd @17:01:56.24 Plunge.seek :pTop @17:01:57.16 delay 5 seconds @17:02:02.19 Air.in :glide @17:02:02.26 Air.seek :fullVent @17:02:04.93 delay 2 seconds @17:02:06.96 Plunge.seek :pBottom @17:02:07.87 Seawater evacuated, Pressure above/below filter released. @17:02:07.90 Plunge.seek :locked @17:02:09.30 Handoff.select :waste @17:02:09.44 Lysis1.in :slow @17:02:09.52 Lysis1.seek 950uL @17:02:23.60 delay 5 seconds @17:02:28.63 Handoff.select :blocked @17:02:28.87 Plunge.seek :puckTop @17:02:29.91 Lysis1.seek 350uL @17:02:57.89 delay 3 seconds @17:03:00.99 Air.in mix with 5.31227 parts Lysis1 @17:03:02.31 Air.seek :evac @17:03:02.36 Lysis1.seek 190uL @17:03:10.08 Air.in :fast @17:03:10.16 Air.seek :full @17:03:16.85 Lysis1.seek :empty @17:03:25.99 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @12.8psi @17:03:26.02 Plunge.seek :puckBottom @17:03:27.09 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @10.4psi @17:03:27.11 Polling Can every 2:00 @17:03:27.69 PuckHeater.configure :default #ambient 22.8C @17:03:27.77 PuckHeater.seek 70.4C @17:04:38.18 PuckHeater.hold 2:00,70.4C @17:06:38.17 Polling Can every 2:00 @17:06:38.73 PuckHeater.configure :default #ambient 22.9C @17:06:38.82 PuckHeater.seek 69.3C @17:06:39.03 PuckHeater.hold 2:00,69.3C @17:08:39.05 Polling Can every 2:00 @17:08:39.60 PuckHeater.configure :default #ambient 23.0C @17:08:39.69 PuckHeater.hold 18:00,68.3C @17:26:38.25 Polling Can every 2:00 @17:26:38.81 PuckHeater.configure :default #ambient 22.8C @17:26:38.88 PuckHeater.seek 104.3C @17:27:39.63 PuckHeater.hold 2:00,104.3C @17:29:39.45 Polling Can every 2:00 @17:29:40.00 PuckHeater.configure :default #ambient 22.8C @17:29:40.09 PuckHeater.seek 103.2C @17:29:40.30 PuckHeater.hold 2:00,103.2C @17:31:40.18 Polling Can every 2:00 @17:31:40.73 PuckHeater.configure :default #ambient 22.8C @17:31:40.82 PuckHeater.hold 2:00,102.2C @17:33:40.81 delay 5:00 @17:38:40.89 Lysate cooked @6.3psi @17:38:40.92 Plunge.seek :puckTop @17:38:41.84 Air syringe pumping up to 5 strokes to reach 40psi @17:38:41.86 Air stroke #1 of 5 @17:38:42.00 Air.in :pressurize @17:38:42.07 Air.seek :pressurized @17:38:48.43 6.4psi after pressurize @17:38:48.46 Air.in :compress @17:38:48.55 Air.seek :evac @17:38:53.71 Reached 44.3 of 40.0psi after pumping 1631uL (0.90 strokes) @17:38:53.74 Air.in :default @17:38:53.85 Air.hold @17:38:53.95 delay 2 seconds @17:38:55.99 Air.in :fast @17:38:56.07 Air.seek :full @17:39:02.13 Plunge.seek :puckBottom @17:39:03.10 Lysis1.seek 138uL @17:39:09.68 delay 2 seconds @17:39:11.72 Plunge.seek :puckTop @17:39:12.62 Lysis1.seek :empty @17:39:19.25 Plunge.seek :puckBottom @17:39:20.35 Lysis1.in :extract @17:39:20.47 Lysis1.seek 675uL,nil @17:39:22.84 Air.in :pressurize @17:39:22.92 Air.seek :pressurized @17:39:30.05 10.3psi after pressurize @17:39:30.12 Air.in :compressLysate @17:39:30.30 Air.seek :evac @17:39:41.68 Reached 15.6 of 15.5psi after pumping 1547uL (0.85 strokes) @17:39:41.70 Air.in :default @17:39:41.79 Air.hold @17:39:42.63 Air.in :compressLysate @17:39:42.75 Air.seek :evac @17:39:43.77 Syringe::LysatePlungerStuck in nadia -- at < 440uL during Lysis Syringe #1 (LyS1) move @17:39:43.82 Lysis1.in :default @17:39:43.91 Lysis1.seek 410uL @17:39:44.76 Reached 16.2 of 15.5psi after pumping 1572uL (0.87 strokes) @17:39:44.78 Air.in :default @17:39:44.86 Air.hold @17:39:45.22 Lysis1.in :extract @17:39:45.34 Lysis1.seek 675uL,nil @17:39:46.97 Air.in :compressLysate @17:39:47.08 Air.seek :evac @17:39:47.44 Syringe::LysatePlungerStuck in nadia -- at < 550uL during Lysis Syringe #1 (LyS1) move @17:39:47.51 Lysis1.in :default @17:39:47.60 Lysis1.seek 520uL @17:39:50.02 Reached 15.8 of 15.5psi after pumping 1610uL (0.89 strokes) @17:39:50.04 Air.in :default @17:39:50.14 Air.hold @17:39:50.31 Lysis1.in :extract @17:39:50.45 Lysis1.seek 675uL,nil @17:39:51.80 Air.in :compressLysate @17:39:51.88 Air.seek :evac @17:39:52.14 Syringe::LysatePlungerStuck in nadia -- at < 584uL during Lysis Syringe #1 (LyS1) move @17:39:52.19 Lysis1.in :default @17:39:52.29 Lysis1.seek 554uL @17:39:53.19 Reached 16.0 of 15.5psi after pumping 1626uL (0.90 strokes) @17:39:53.21 Air.in :default @17:39:53.31 Air.hold @17:39:53.60 Lysis1.in :extract @17:39:53.68 Lysis1.seek 675uL,nil @17:39:55.90 Air.in :compressLysate @17:39:55.99 Air.seek :evac @17:39:56.27 Syringe::LysatePlungerStuck in nadia -- at < 667uL during Lysis Syringe #1 (LyS1) move @17:39:56.32 Lysis1.in :default @17:39:56.43 Lysis1.seek 637uL @17:39:58.92 Reached 15.7 of 15.5psi after pumping 1665uL (0.92 strokes) @17:39:58.94 Air.in :default @17:39:59.05 Air.hold @17:39:59.22 Lysis1.in :extract @17:39:59.33 Lysis1.seek 675uL,nil @17:40:00.87 Pulled 675uL lysate @15.0psi @17:40:01.09 Lysis1.seek 800uL,nil @17:40:01.36 Air.in :compressLysate @17:40:01.44 Air.seek :evac @17:40:07.45 Air.in :default @17:40:07.63 Air.hold @17:40:07.85 Air.in :glide @17:40:07.92 Air.seek :fullVent @17:40:09.97 Handoff.select :waste @17:40:10.12 Recovered 800uL lysate @14.1psi @17:40:10.14 delay 3 seconds @17:40:13.24 After 3 delay Recovered 800uL lysate @-1.5psi @17:40:13.26 delay 5 seconds @17:40:18.32 Gate.power :heat,:OFF @17:40:18.36 Gate.power :cartridge,:OFF @17:40:18.47 PinPort.in :unlocking @17:40:18.55 PinPort.seek :clear @17:40:19.05 Archive.in :unlocking @17:40:19.18 Archive.seek :clear @17:40:19.31 Air.in :unlocking @17:40:19.43 Air.seek :clear @17:40:19.60 Lysis2.in :unlocking @17:40:19.69 Lysis2.seek :clear @17:40:24.43 Lysis1.in :unlocking @17:40:24.62 Lysis1.seek :clear @17:40:24.72 Plunge.in :unlocking @17:40:24.86 Plunge.seek :clear @17:43:15.21 -> cleanPortable @17:43:15.66 Waiting for nadia to answer "LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue" @17:43:51.74 Answered LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue"" @17:43:51.85 Lysis1.in :fast @17:43:51.94 Lysis1.seek :locked @17:43:54.50 Plunge.in :default @17:43:54.62 Plunge.seek :locked @17:43:54.80 Air.in :fast @17:43:54.89 Air.seek :locked @17:43:55.87 Powering cartridge bus @17:43:58.57 Gate.power :cartridge,:OFF @17:43:59.14 Mounted archive Cartridge @17:43:59.19 Toroid.seek :bypassed @17:43:59.30 Priming sample loop w/10ml, bypass w/10ml @17:43:59.32 SamplePump.setPosition! 0ml @17:43:59.40 Bypass.open @17:44:01.06 Intake.open @17:44:02.01 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:44:04.65 Vacuum tare is 54% of full scale at 23C (drifted 2%) @17:44:04.89 Abs.in :default @17:44:04.97 Abs.hold @17:44:05.24 SamplePump.in :start @17:44:05.33 SamplePump.seek 10ml,1:10 @17:44:05.93 SamplePump.configure :prime @17:44:17.61 Abs.stop @17:44:17.69 SamplePump.setPosition! 0ml @17:44:17.79 Exhaust.open @17:44:19.39 Bypass.close @17:44:21.10 Abs.in :default @17:44:21.19 Abs.hold @17:44:21.53 SamplePump.in :start @17:44:21.63 SamplePump.seek 10ml,1:10 @17:44:22.22 SamplePump.configure :prime @17:44:33.90 Abs.stop @17:44:33.98 Waiting for nadia to answer "INTAKE line plumbed to 10% Bleach, push return to continue" @17:45:13.16 Answered INTAKE line plumbed to 10% Bleach, push return to continue"" @17:45:13.19 Toroid.seek :bypassed @17:45:13.33 Priming sample loop w/10ml, bypass w/10ml @17:45:13.35 SamplePump.setPosition! 0ml @17:45:13.44 Exhaust.close @17:45:14.26 Bypass.open @17:45:16.09 Abs.in :default @17:45:16.17 Abs.hold @17:45:16.43 SamplePump.in :start @17:45:16.51 SamplePump.seek 10ml,1:10 @17:45:17.12 SamplePump.configure :prime @17:45:28.80 Abs.stop @17:45:28.88 SamplePump.setPosition! 0ml @17:45:28.96 Exhaust.open @17:45:30.52 Bypass.close @17:45:32.21 Abs.in :default @17:45:32.30 Abs.hold @17:45:32.63 SamplePump.in :start @17:45:32.76 SamplePump.seek 10ml,1:10 @17:45:33.32 SamplePump.configure :prime @17:45:45.04 Abs.stop @17:45:45.12 Toroid.seek :cartridge @17:45:50.15 Sampling 25.0ml @17:45:50.17 SamplePump.setPosition! 0ml @17:45:50.45 Abs.in :default @17:45:50.54 Abs.hold @17:45:50.92 SamplePump.in :start @17:45:51.02 SamplePump.seek 25ml,2:10 @17:45:51.61 SamplePump.configure :sample @17:46:21.08 Abs.stop @17:46:21.16 Intake.close @17:46:21.30 Exhaust.close @17:46:21.37 Handoff.select :close @17:46:21.40 Toroid.seek :bypassed @17:46:25.89 Sampled 25.0ml @17:46:25.92 delay 10:00 @17:56:25.97 Waiting for nadia to answer "Move INTAKE line to MillQ water" @17:56:26.01 Answered Move INTAKE line to MillQ water"" @17:56:26.04 Toroid.seek :bypassed @17:56:26.17 Priming sample loop w/50ml, bypass w/25ml @17:56:26.20 SamplePump.setPosition! 0ml @17:56:26.28 Bypass.open @17:56:28.05 Intake.open @17:56:29.79 Abs.in :default @17:56:29.88 Abs.hold @17:56:30.14 SamplePump.in :start @17:56:30.24 SamplePump.seek 25ml,2:10 @17:56:30.83 SamplePump.configure :prime @17:57:00.28 Abs.stop @17:57:00.36 SamplePump.setPosition! 0ml @17:57:00.45 Exhaust.open @17:57:02.01 Bypass.close @17:57:03.72 Abs.in :default @17:57:03.79 Abs.hold @17:57:04.13 SamplePump.in :start @17:57:04.22 SamplePump.seek 50ml,3:50 @17:57:04.82 SamplePump.configure :prime @17:58:03.88 Abs.stop @17:58:03.96 Toroid.seek :cartridge @17:58:08.33 Sampling 25.0ml @17:58:08.35 SamplePump.setPosition! 0ml @17:58:08.61 Abs.in :default @17:58:08.71 Abs.hold @17:58:09.71 SamplePump.in :start @17:58:09.81 SamplePump.seek 25ml,2:35 @17:58:10.40 SamplePump.configure :sample @17:58:39.86 Abs.stop @17:58:39.95 Intake.close @17:58:40.03 Exhaust.close @17:58:40.18 Toroid.seek :bypassed @17:58:44.56 Sampled 25.0ml @17:58:44.87 Air.in :unlocking @17:58:45.01 Air.seek :clear @17:58:46.05 Lysis1.in :unlocking @17:58:46.22 Lysis1.seek :clear @17:58:46.29 Plunge.in :unlocking @17:58:46.41 Plunge.seek :clear @17:58:47.55 Portable sample Path clean, ready for next sample @18:34:53.39 -> ESP.reready! @18:34:53.45 Plunge.in :default @18:34:53.56 Toroid.in :default @18:34:53.64 PinPort.in :default @18:34:53.71 Archive.in :default @18:34:53.79 Plunge.home.jog 0 @18:34:53.89 Air.in :default @18:34:53.95 Lysis1.in :default @18:34:54.07 Lysis2.in :default @18:34:54.22 Toroid.home.jog 0 @18:34:54.29 Archive.home.jog 0 @18:34:54.39 PinPort.home.jog 0 @18:34:54.60 Air.home.jog 0 @18:34:54.64 Lysis2.home.jog 0 @18:34:54.73 Lysis1.home.jog 0 @18:34:55.53 Plunge.in :homing @18:34:55.74 Plunge.jog 561 @18:34:55.95 Lysis2.in :homing @18:34:56.01 Lysis1.in :homing @18:34:56.05 Archive.in :homing @18:34:56.11 Toroid.in :homing @18:34:56.15 Air.in :homing @18:34:56.18 PinPort.in :homing @18:34:56.46 Lysis2.jog 1613 @18:34:56.55 Lysis1.jog 1661 @18:34:56.60 Archive.jog 1615 @18:34:56.70 Air.jog 1568 @18:34:56.73 Toroid.jog 268 @18:34:56.84 PinPort.jog -707 @18:34:57.20 Plunge.setRawPosition! 50 @18:34:57.27 Plunge.seek :home @18:34:57.55 Plunge.in :default @18:34:57.97 Toroid.setRawPosition! 0 @18:34:58.05 Toroid.seek :home @18:34:58.22 Lysis2.setRawPosition! 50 @18:34:58.25 Lysis1.setRawPosition! 50 @18:34:58.29 PinPort.setRawPosition! -50 @18:34:59.01 Toroid.in :default @18:34:59.24 Lysis1.seek :home @18:34:59.34 Lysis2.seek :home @18:34:59.39 PinPort.seek :home @18:34:59.68 Air.setRawPosition! 50 @18:34:59.70 Archive.setRawPosition! 50 @18:34:59.88 Air.seek :home @18:34:59.90 Archive.seek :home @18:35:00.16 Lysis2.in :default @18:35:00.20 Lysis1.in :default @18:35:00.25 PinPort.in :default @18:35:00.49 Archive.in :default @18:35:00.51 Air.in :default @18:35:00.94 Toroid.seek :clear @18:35:01.14 PinPort.in :unlocking @18:35:01.22 PinPort.seek :clear @18:35:03.22 Air.seek :clear @18:35:03.89 Lysis1.seek :clear @18:35:04.81 Lysis2.seek :clear @18:35:05.61 Archive.seek :clear Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 25ml Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Abs = Absolute Pressure (Leak) Sensor @12psia Toroid = Toroid Valve (TV) clear PinPort = Handoff Pin Port (PP) clear Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear @18:35:22.95 -> Log.comment "Ano Nuevo Sample, 30m, 5um filter" @18:35:22.97 Ano Nuevo Sample, 30m, 5um filter @18:35:30.16 -> BACsampler.goalvolume @18:35:30.19 NoMethodError in nadia -- undefined method `goalvolume' for BACsampler:Cartridge::Sampler @18:35:41.99 -> BACsampler.goalVolume 5000 @18:35:49.54 -> Cart.fire @18:35:49.66 Lysis1.in :fast @18:35:49.74 Lysis1.seek :locked @18:35:52.30 Plunge.seek :locked @18:35:52.50 Air.in :fast @18:35:52.59 Air.seek :locked @18:35:53.58 Powering cartridge bus @18:35:56.29 PuckHeater.configure :default #ambient 22.8C @18:35:56.38 Gate.power :heat,:ON,limit to 5000mA @18:35:57.08 Mounted lysis Cartridge board#J59940 @18:35:57.14 Using default cartridge heat loss curve for HeatingFunctionInStage @18:35:57.39 Air.in :default @18:35:57.48 Toroid.seek :bypassed @18:35:57.52 Air.seek :sealed @18:35:59.80 Priming sample loop w/25ml, bypass w/10ml @18:35:59.82 SamplePump.setPosition! 0ml @18:35:59.91 Bypass.open @18:36:01.57 Intake.open @18:36:02.55 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @18:36:05.17 Vacuum tare is 53% of full scale at 22C (drifted 1%) @18:36:05.36 Abs.in :default @18:36:05.45 Abs.hold @18:36:05.72 SamplePump.in :start @18:36:05.82 SamplePump.seek 10ml,1:10 @18:36:06.41 SamplePump.configure :prime @18:36:18.09 Abs.stop @18:36:18.17 SamplePump.setPosition! 0ml @18:36:18.24 Exhaust.open @18:36:19.80 Bypass.close @18:36:21.52 Abs.in :default @18:36:21.61 Abs.hold @18:36:21.94 SamplePump.in :start @18:36:22.03 SamplePump.seek 25ml,2:10 @18:36:22.62 SamplePump.configure :prime @18:36:52.05 Abs.stop @18:36:52.14 Bypass.open @18:36:52.99 Intake.close @18:36:53.91 Toroid.seek :crack @18:36:58.20 Toroid.seek :cartridge @18:36:59.31 Pressurizing cartridge to 26psi above 13.3psia @18:36:59.33 SamplePump.setPosition! 0ml @18:36:59.41 Intake.open @18:37:00.32 Bypass.close @18:37:01.19 Exhaust.close @18:37:02.19 Abs.in :default @18:37:02.28 Abs.hold @18:37:02.54 SamplePump.in :charge @18:37:02.64 SamplePump.seek 2.3ml,40 seconds @18:37:18.82 Abs.stop @18:37:18.90 Intake.close @18:37:19.86 Pressurized cartridge with 1.198ml to 26.0psi @18:37:19.88 Isolated @39.8psia (26.5psi) @18:37:19.93 Verifying cartridge holds at least 0.65..0.85 of 39.8psia @18:37:19.99 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @18:37:24.62 Toroid.seek :cartridge @18:37:24.78 Sampling 5000.0ml @18:37:24.81 SamplePump.setPosition! 0ml @18:37:24.91 Exhaust.open @18:37:25.72 Intake.open @18:37:26.84 Abs.in :default @18:37:26.92 Abs.hold @18:37:27.27 SamplePump.in :start @18:37:27.35 SamplePump.seek 5000ml,5:33:50 @18:37:27.96 SamplePump.configure :sample @18:37:30.38 filtering 2/5000ml at 0.84ml/s with 8.3psi vacuum @18:38:30.42 filtering 53/5000ml at 0.85ml/s with 8.1psi vacuum @18:39:30.42 filtering 104/5000ml at 0.84ml/s with 7.9psi vacuum @18:40:30.42 filtering 154/5000ml at 0.84ml/s with 9.1psi vacuum @18:41:30.43 filtering 205/5000ml at 0.84ml/s with 8.6psi vacuum @18:42:30.43 filtering 255/5000ml at 0.84ml/s with 8.1psi vacuum @18:43:30.42 filtering 306/5000ml at 0.84ml/s with 9.3psi vacuum @18:44:30.42 filtering 357/5000ml at 0.84ml/s with 8.6psi vacuum @18:45:30.42 filtering 407/5000ml at 0.84ml/s with 8.8psi vacuum @18:46:30.42 filtering 458/5000ml at 0.84ml/s with 10.2psi vacuum @18:47:30.42 filtering 509/5000ml at 0.84ml/s with 7.6psi vacuum @18:48:30.42 filtering 559/5000ml at 0.84ml/s with 5.7psi vacuum @18:49:30.42 filtering 610/5000ml at 0.84ml/s with 7.8psi vacuum @18:50:30.42 filtering 660/5000ml at 0.84ml/s with 10.8psi vacuum @18:51:30.42 filtering 711/5000ml at 0.84ml/s with 9.5psi vacuum @18:52:30.42 filtering 762/5000ml at 0.84ml/s with 10.3psi vacuum @18:53:30.42 filtering 812/5000ml at 0.84ml/s with 6.4psi vacuum @18:54:30.42 filtering 863/5000ml at 0.84ml/s with 9.9psi vacuum @18:55:30.43 filtering 913/5000ml at 0.84ml/s with 10.3psi vacuum @18:56:30.42 filtering 964/5000ml at 0.84ml/s with 10.4psi vacuum @18:57:30.42 filtering 1015/5000ml at 0.84ml/s with 11.6psi vacuum @18:58:30.44 filtering 1065/5000ml at 0.84ml/s with 9.5psi vacuum @18:59:30.43 filtering 1116/5000ml at 0.84ml/s with 8.0psi vacuum @19:00:30.43 filtering 1166/5000ml at 0.84ml/s with 6.7psi vacuum @19:01:30.43 filtering 1217/5000ml at 0.84ml/s with 11.3psi vacuum @19:02:30.43 filtering 1268/5000ml at 0.84ml/s with 10.9psi vacuum @19:03:30.43 filtering 1318/5000ml at 0.84ml/s with 11.1psi vacuum @19:04:30.42 filtering 1369/5000ml at 0.84ml/s with 11.3psi vacuum @19:05:30.42 filtering 1420/5000ml at 0.84ml/s with 11.0psi vacuum @19:06:30.43 filtering 1470/5000ml at 0.84ml/s with 10.8psi vacuum @19:07:30.42 filtering 1521/5000ml at 0.84ml/s with 11.6psi vacuum @19:08:30.42 filtering 1572/5000ml at 0.84ml/s with 11.4psi vacuum @19:09:30.42 filtering 1622/5000ml at 0.84ml/s with 11.8psi vacuum @19:10:30.42 filtering 1673/5000ml at 0.84ml/s with 12.4psi vacuum @19:11:30.42 filtering 1723/5000ml at 0.84ml/s with 10.7psi vacuum @19:12:30.43 filtering 1774/5000ml at 0.84ml/s with 12.8psi vacuum @19:13:30.42 filtering 1825/5000ml at 0.84ml/s with 10.6psi vacuum @19:14:30.42 filtering 1875/5000ml at 0.84ml/s with 11.7psi vacuum @19:15:30.42 filtering 1926/5000ml at 0.84ml/s with 12.9psi vacuum @19:16:30.42 filtering 1977/5000ml at 0.84ml/s with 11.1psi vacuum @19:17:30.43 filtering 2027/5000ml at 0.84ml/s with 12.4psi vacuum @19:18:30.42 filtering 2078/5000ml at 0.84ml/s with 12.6psi vacuum @19:19:30.43 filtering 2129/5000ml at 0.84ml/s with 11.4psi vacuum @19:20:30.42 filtering 2179/5000ml at 0.84ml/s with 11.9psi vacuum @19:21:30.43 filtering 2230/5000ml at 0.84ml/s with 12.8psi vacuum @19:22:30.42 filtering 2280/5000ml at 0.84ml/s with 10.8psi vacuum @19:23:30.42 filtering 2331/5000ml at 0.84ml/s with 13.1psi vacuum @19:24:30.43 filtering 2382/5000ml at 0.84ml/s with 13.8psi vacuum @19:25:30.42 filtering 2432/5000ml at 0.84ml/s with 12.9psi vacuum @19:26:30.42 filtering 2483/5000ml at 0.84ml/s with 10.4psi vacuum @19:27:30.42 filtering 2534/5000ml at 0.84ml/s with 14.0psi vacuum @19:28:30.42 filtering 2584/5000ml at 0.84ml/s with 13.4psi vacuum @19:29:30.44 filtering 2635/5000ml at 0.84ml/s with 13.7psi vacuum @19:30:30.42 filtering 2686/5000ml at 0.84ml/s with 14.7psi vacuum @19:31:30.44 filtering 2736/5000ml at 0.84ml/s with 13.5psi vacuum @19:32:30.42 filtering 2787/5000ml at 0.84ml/s with 14.1psi vacuum @19:33:30.42 filtering 2837/5000ml at 0.84ml/s with 14.9psi vacuum @19:34:30.42 filtering 2888/5000ml at 0.84ml/s with 14.2psi vacuum @19:35:30.42 filtering 2939/5000ml at 0.84ml/s with 11.9psi vacuum @19:36:30.43 filtering 2989/5000ml at 0.84ml/s with 11.9psi vacuum @19:37:30.43 filtering 3040/5000ml at 0.84ml/s with 13.5psi vacuum @19:38:30.43 filtering 3091/5000ml at 0.84ml/s with 14.0psi vacuum @19:39:30.42 filtering 3141/5000ml at 0.84ml/s with 13.0psi vacuum @19:40:30.43 filtering 3192/5000ml at 0.84ml/s with 14.7psi vacuum @19:41:30.42 filtering 3243/5000ml at 0.84ml/s with 16.2psi vacuum @19:42:30.43 filtering 3293/5000ml at 0.84ml/s with 12.2psi vacuum @19:43:30.42 filtering 3344/5000ml at 0.84ml/s with 11.1psi vacuum @19:44:30.42 filtering 3395/5000ml at 0.84ml/s with 12.1psi vacuum @19:45:30.42 filtering 3445/5000ml at 0.84ml/s with 13.8psi vacuum @19:46:30.42 filtering 3496/5000ml at 0.84ml/s with 14.9psi vacuum @19:47:30.42 filtering 3546/5000ml at 0.84ml/s with 14.8psi vacuum @19:48:30.42 filtering 3597/5000ml at 0.84ml/s with 12.6psi vacuum @19:49:30.42 filtering 3648/5000ml at 0.84ml/s with 14.6psi vacuum @19:50:30.43 filtering 3699/5000ml at 0.84ml/s with 14.2psi vacuum @19:51:30.41 filtering 3749/5000ml at 0.84ml/s with 14.0psi vacuum @19:52:30.42 filtering 3800/5000ml at 0.84ml/s with 16.1psi vacuum @19:53:30.41 filtering 3851/5000ml at 0.84ml/s with 13.6psi vacuum @19:54:30.42 filtering 3901/5000ml at 0.84ml/s with 16.4psi vacuum @19:55:30.42 filtering 3952/5000ml at 0.84ml/s with 18.3psi vacuum @19:56:30.42 filtering 4003/5000ml at 0.84ml/s with 15.1psi vacuum @19:57:30.43 filtering 4053/5000ml at 0.84ml/s with 16.9psi vacuum @19:58:30.42 filtering 4104/5000ml at 0.84ml/s with 15.7psi vacuum @19:59:30.45 filtering 4154/5000ml at 0.84ml/s with 17.1psi vacuum @20:00:30.42 filtering 4205/5000ml at 0.84ml/s with 14.6psi vacuum @20:01:30.43 filtering 4256/5000ml at 0.84ml/s with 16.7psi vacuum @20:02:30.42 filtering 4306/5000ml at 0.84ml/s with 17.1psi vacuum @20:03:30.42 filtering 4357/5000ml at 0.84ml/s with 14.9psi vacuum @20:04:30.43 filtering 4408/5000ml at 0.84ml/s with 17.4psi vacuum @20:05:30.42 filtering 4458/5000ml at 0.84ml/s with 18.4psi vacuum @20:06:30.43 filtering 4509/5000ml at 0.84ml/s with 15.0psi vacuum @20:07:30.42 filtering 4560/5000ml at 0.84ml/s with 14.9psi vacuum @20:08:30.43 filtering 4610/5000ml at 0.84ml/s with 14.0psi vacuum @20:09:30.42 filtering 4661/5000ml at 0.84ml/s with 17.1psi vacuum @20:10:30.43 filtering 4712/5000ml at 0.84ml/s with 18.9psi vacuum @20:11:30.43 filtering 4762/5000ml at 0.84ml/s with 16.7psi vacuum @20:12:30.42 filtering 4813/5000ml at 0.84ml/s with 18.0psi vacuum @20:13:30.42 filtering 4864/5000ml at 0.84ml/s with 15.3psi vacuum @20:14:30.43 filtering 4914/5000ml at 0.84ml/s with 17.6psi vacuum @20:15:30.41 filtering 4965/5000ml at 0.84ml/s with 17.4psi vacuum @20:16:12.03 Abs.stop @20:16:12.15 Intake.close @20:16:12.21 Exhaust.close @20:16:12.35 Toroid.seek :bypassed @20:16:16.77 Sampled 5000.0ml @20:16:16.83 Toroid.seek :bypassed @20:16:16.96 delay 5 seconds @20:16:21.99 Handoff.select :waste @20:16:22.16 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @20:16:28.80 Syringe tare is 52% of full scale at 23C (drifted 1%) @20:16:28.84 PinPort.in :locking @20:16:28.96 PinPort.seek :locked @20:16:29.39 Archive.in :fast @20:16:29.51 Lysis2.in :fast @20:16:29.57 Lysis1.seek 1250uL @20:16:29.66 Archive.seek :empty @20:16:29.81 Lysis2.seek :empty @20:16:38.22 Evacuating puck with filter bubble point of 50psi @20:16:38.25 PinPort.seek :locked @20:16:38.41 Handoff.select :waste @20:16:38.47 Plunge.seek :puckBottom @20:16:39.85 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @20:16:46.45 Air syringe pumping up to 1 strokes to reach 8psi @20:16:46.47 Air stroke #1 of 1 @20:16:46.60 Air.in :pressurize @20:16:46.73 Air.seek :pressurized @20:16:52.09 0.3psi after pressurize @20:16:52.12 Air.in :compress @20:16:52.23 Air.seek :evac @20:16:59.83 3.6psi after compress @20:17:11.92 0.3psi after compress (stable) @20:17:11.94 Air.in :default @20:17:12.04 Air.hold @20:17:12.28 at 0.0psi Opened to waste @20:17:12.30 Handoff.select :blocked @20:17:12.48 Plunge.seek :puckTop @20:17:13.39 Air syringe pumping up to 8 strokes to reach 50psi @20:17:13.40 Air stroke #1 of 8 @20:17:13.54 Air.in :compress @20:17:13.63 Air.seek :evac @20:17:13.81 0.4psi after compress @20:17:24.40 0.6psi after compress (stable) @20:17:24.50 Air.in :gulp @20:17:24.60 Air.seek :topVent @20:17:31.25 0.5psi after gulp @20:17:37.35 0.6psi after gulp (stable) @20:17:37.36 Air stroke #2 of 8 @20:17:37.50 Air.in :pressurize @20:17:37.59 Air.seek :pressurized @20:17:43.97 0.7psi after pressurize @20:17:44.00 Air.in :compress @20:17:44.12 Air.seek :evac @20:17:51.73 14.3psi after compress @20:18:02.82 16.0psi after compress (stable) @20:18:02.94 Air.in :gulp @20:18:03.07 Air.seek :topVent @20:18:09.71 15.8psi after gulp @20:18:15.30 15.8psi after gulp (stable) @20:18:15.31 Air stroke #3 of 8 @20:18:15.46 Air.in :pressurize @20:18:15.54 Air.seek :pressurized @20:18:21.91 15.9psi after pressurize @20:18:21.94 Air.in :compress @20:18:22.05 Air.seek :evac @20:18:29.68 28.0psi after compress @20:18:40.27 29.7psi after compress (stable) @20:18:40.30 Air.in :gulp @20:18:40.43 Air.seek :topVent @20:18:47.07 29.8psi after gulp @20:18:52.78 29.8psi after gulp (stable) @20:18:52.79 Air stroke #4 of 8 @20:18:52.94 Air.in :pressurize @20:18:53.03 Air.seek :pressurized @20:18:59.40 29.6psi after pressurize @20:18:59.43 Air.in :compress @20:18:59.55 Air.seek :evac @20:19:07.15 41.5psi after compress @20:19:17.76 42.9psi after compress (stable) @20:19:17.79 Air.in :gulp @20:19:17.91 Air.seek :topVent @20:19:24.54 42.9psi after gulp @20:19:30.13 42.9psi after gulp (stable) @20:19:30.23 Air stroke #5 of 8 @20:19:30.38 Air.in :pressurize @20:19:30.45 Air.seek :pressurized @20:19:36.81 42.8psi after pressurize @20:19:36.84 Air.in :compress @20:19:36.96 Air.seek :evac @20:19:43.79 Reached 50.6 of 50.0psi after pumping 9008uL (4.97 strokes) @20:19:43.81 Air.in :default @20:19:43.92 Air.hold @20:19:44.04 Plunge.seek :puckBottom @20:19:44.95 delay 3 seconds @20:19:47.97 Handoff.select :waste @20:19:48.13 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @20:19:55.72 delay 1:00 @20:20:55.75 Handoff.select :blocked @20:20:55.90 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @20:21:18.49 Handoff.select :waste @20:21:18.65 -0.1psi at evacEnd @20:21:18.69 Handoff.select :blocked @20:21:18.88 Plunge.seek :puckTop @20:21:19.79 Air.in :fast @20:21:19.89 Air.seek :topVent @20:21:26.41 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @20:21:33.00 Stable @1.0psi after Handoff blocked @20:21:33.03 Air syringe pumping up to 8 strokes to reach 35psi @20:21:33.06 Air stroke #1 of 8 @20:21:33.21 Air.in :pressurize @20:21:33.30 Air.seek :pressurized @20:21:39.67 1.1psi after pressurize @20:21:39.69 Air.in :compress @20:21:39.81 Air.seek :evac @20:21:47.43 14.4psi after compress @20:21:58.66 16.0psi after compress (stable) @20:21:58.72 Air.in :gulp @20:21:58.82 Air.seek :topVent @20:22:05.48 15.9psi after gulp @20:22:11.07 16.0psi after gulp (stable) @20:22:11.09 Air stroke #2 of 8 @20:22:11.23 Air.in :pressurize @20:22:11.33 Air.seek :pressurized @20:22:17.69 15.8psi after pressurize @20:22:17.72 Air.in :compress @20:22:17.85 Air.seek :evac @20:22:25.45 27.9psi after compress @20:22:36.07 29.6psi after compress (stable) @20:22:36.17 Air.in :gulp @20:22:36.29 Air.seek :topVent @20:22:42.93 29.6psi after gulp @20:22:49.53 29.6psi after gulp (stable) @20:22:49.55 Air stroke #3 of 8 @20:22:49.69 Air.in :pressurize @20:22:49.78 Air.seek :pressurized @20:22:56.14 29.4psi after pressurize @20:22:56.17 Air.in :compress @20:22:56.26 Air.seek :evac @20:23:02.24 Reached 35.3 of 35.0psi after pumping 5338uL (2.95 strokes) @20:23:02.27 Air.in :default @20:23:02.37 Air.hold @20:23:02.49 Plunge.seek :puckBottom @20:23:03.41 delay 3 seconds @20:23:06.43 Handoff.select :waste @20:23:06.59 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @20:23:14.18 delay 1:00 @20:24:14.22 Handoff.select :blocked @20:24:14.39 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @20:24:37.00 Handoff.select :waste @20:24:37.73 -0.3psi at evacEnd @20:24:37.79 Plunge.seek :pTop @20:24:38.73 delay 5 seconds @20:24:43.76 Air.in :glide @20:24:43.83 Air.seek :fullVent @20:24:46.24 delay 2 seconds @20:24:48.27 Plunge.seek :pBottom @20:24:49.17 Seawater evacuated, Pressure above/below filter released. @20:24:49.20 Plunge.seek :locked @20:24:50.60 Handoff.select :waste @20:24:50.76 Lysis1.in :slow @20:24:50.85 Lysis1.seek 950uL @20:25:04.94 delay 5 seconds @20:25:09.96 Handoff.select :blocked @20:25:10.14 Plunge.seek :puckTop @20:25:11.19 Lysis1.seek 350uL @20:25:39.16 delay 3 seconds @20:25:42.28 Air.in mix with 5.31227 parts Lysis1 @20:25:43.12 Air.seek :evac @20:25:43.22 Lysis1.seek 190uL @20:25:50.89 Air.in :fast @20:25:50.99 Air.seek :full @20:25:57.67 Lysis1.seek :empty @20:26:06.83 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @12.2psi @20:26:06.85 Plunge.seek :puckBottom @20:26:07.91 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @10.7psi @20:26:07.93 Polling Can every 2:00 @20:26:08.51 PuckHeater.configure :default #ambient 22.9C @20:26:08.58 PuckHeater.seek 70.4C @20:27:18.98 PuckHeater.hold 2:00,70.4C @20:29:18.40 Polling Can every 2:00 @20:29:18.95 PuckHeater.configure :default #ambient 22.9C @20:29:19.03 PuckHeater.seek 69.3C @20:29:19.24 PuckHeater.hold 2:00,69.3C @20:31:18.63 Polling Can every 2:00 @20:31:19.18 PuckHeater.configure :default #ambient 22.9C @20:31:19.27 PuckHeater.hold 18:00,68.3C @20:49:12.30 Polling Can every 2:00 @20:49:12.85 PuckHeater.configure :default #ambient 23.0C @20:49:12.94 PuckHeater.seek 104.3C @20:50:13.64 PuckHeater.hold 2:00,104.3C @20:52:13.05 Polling Can every 2:00 @20:52:13.60 PuckHeater.configure :default #ambient 23.1C @20:52:13.69 PuckHeater.seek 103.2C @20:52:13.88 PuckHeater.hold 2:00,103.2C @20:54:13.18 Polling Can every 2:00 @20:54:13.73 PuckHeater.configure :default #ambient 23.1C @20:54:13.82 PuckHeater.hold 2:00,102.2C @20:56:13.18 delay 5:00 @21:01:13.26 Lysate cooked @8.3psi @21:01:13.29 Plunge.seek :puckTop @21:01:14.24 Air syringe pumping up to 5 strokes to reach 40psi @21:01:14.26 Air stroke #1 of 5 @21:01:14.39 Air.in :pressurize @21:01:14.48 Air.seek :pressurized @21:01:20.84 8.5psi after pressurize @21:01:20.87 Air.in :compress @21:01:21.04 Air.seek :evac @21:01:27.18 Reached 45.6 of 40.0psi after pumping 1684uL (0.93 strokes) @21:01:27.20 Air.in :default @21:01:27.30 Air.hold @21:01:27.43 delay 2 seconds @21:01:29.46 Air.in :fast @21:01:29.54 Air.seek :full @21:01:35.77 Plunge.seek :puckBottom @21:01:36.80 Lysis1.seek 138uL @21:01:43.38 delay 2 seconds @21:01:45.42 Plunge.seek :puckTop @21:01:46.33 Lysis1.seek :empty @21:01:52.93 Plunge.seek :puckBottom @21:01:54.01 Lysis1.in :extract @21:01:54.13 Lysis1.seek 675uL,nil @21:01:57.24 Air.in :pressurize @21:01:57.35 Air.seek :pressurized @21:02:03.73 10.6psi after pressurize @21:02:03.76 Air.in :compressLysate @21:02:03.88 Air.seek :evac @21:02:24.13 Air.in :default @21:02:24.26 Air.hold @21:02:24.54 Pulled 675uL lysate @14.5psi @21:02:24.79 Lysis1.seek 800uL,nil @21:02:25.04 Air.in :compressLysate @21:02:25.14 Air.seek :evac @21:02:29.27 Air.in :default @21:02:29.35 Air.hold @21:02:29.55 Air.in :glide @21:02:29.64 Air.seek :fullVent @21:02:31.83 Handoff.select :waste @21:02:32.00 Recovered 800uL lysate @14.0psi @21:02:32.03 delay 3 seconds @21:02:35.12 After 3 delay Recovered 800uL lysate @-0.9psi @21:02:35.14 delay 5 seconds @21:02:40.20 Gate.power :heat,:OFF @21:02:40.24 Gate.power :cartridge,:OFF @21:02:40.35 PinPort.in :unlocking @21:02:40.42 PinPort.seek :clear @21:02:41.64 Archive.in :unlocking @21:02:41.79 Archive.seek :clear @21:02:41.91 Air.in :unlocking @21:02:42.06 Air.seek :clear @21:02:42.19 Lysis2.in :unlocking @21:02:42.28 Lysis2.seek :clear @21:02:47.01 Lysis1.in :unlocking @21:02:47.19 Lysis1.seek :clear @21:02:47.26 Plunge.in :unlocking @21:02:47.41 Plunge.seek :clear @21:05:22.43 -> cleanPortable @21:05:22.92 Waiting for nadia to answer "LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue" @21:05:24.18 Answered LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue"" @21:05:24.30 Lysis1.in :fast @21:05:24.38 Lysis1.seek :locked @21:05:26.94 Plunge.in :default @21:05:27.01 Plunge.seek :locked @21:05:27.21 Air.in :fast @21:05:27.30 Air.seek :locked @21:05:28.29 Powering cartridge bus @21:05:30.96 Gate.power :cartridge,:OFF @21:05:31.53 Mounted archive Cartridge @21:05:31.59 Toroid.seek :bypassed @21:05:31.70 Priming sample loop w/10ml, bypass w/10ml @21:05:31.72 SamplePump.setPosition! 0ml @21:05:31.80 Bypass.open @21:05:33.50 Intake.open @21:05:34.47 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:05:37.08 Vacuum tare is 53% of full scale at 23C (drifted 2%) @21:05:37.29 Abs.in :default @21:05:37.38 Abs.hold @21:05:37.64 SamplePump.in :start @21:05:37.73 SamplePump.seek 10ml,1:10 @21:05:38.33 SamplePump.configure :prime @21:05:50.00 Abs.stop @21:05:50.08 SamplePump.setPosition! 0ml @21:05:50.16 Exhaust.open @21:05:52.41 Bypass.close @21:05:54.09 Abs.in :default @21:05:54.17 Abs.hold @21:05:54.53 SamplePump.in :start @21:05:54.62 SamplePump.seek 10ml,1:10 @21:05:55.22 SamplePump.configure :prime @21:06:06.92 Abs.stop @21:06:07.01 Waiting for nadia to answer "INTAKE line plumbed to 10% Bleach, push return to continue" @21:06:41.60 Answered INTAKE line plumbed to 10% Bleach, push return to continue"" @21:06:41.62 Toroid.seek :bypassed @21:06:41.76 Priming sample loop w/10ml, bypass w/10ml @21:06:41.78 SamplePump.setPosition! 0ml @21:06:41.87 Exhaust.close @21:06:42.72 Bypass.open @21:06:44.57 Abs.in :default @21:06:44.64 Abs.hold @21:06:44.97 SamplePump.in :start @21:06:45.06 SamplePump.seek 10ml,1:10 @21:06:45.66 SamplePump.configure :prime @21:06:57.33 Abs.stop @21:06:57.41 SamplePump.setPosition! 0ml @21:06:57.50 Exhaust.open @21:06:59.08 Bypass.close @21:07:00.83 Abs.in :default @21:07:00.90 Abs.hold @21:07:01.24 SamplePump.in :start @21:07:01.34 SamplePump.seek 10ml,1:10 @21:07:01.93 SamplePump.configure :prime @21:07:13.60 Abs.stop @21:07:13.68 Toroid.seek :cartridge @21:07:18.05 Sampling 25.0ml @21:07:18.07 SamplePump.setPosition! 0ml @21:07:18.33 Abs.in :default @21:07:18.41 Abs.hold @21:07:18.78 SamplePump.in :start @21:07:18.87 SamplePump.seek 25ml,2:10 @21:07:19.47 SamplePump.configure :sample @21:07:48.90 Abs.stop @21:07:49.01 Intake.close @21:07:49.06 Exhaust.close @21:07:49.16 Handoff.select :close @21:07:49.98 Toroid.seek :bypassed @21:07:54.41 Sampled 25.0ml @21:07:54.43 delay 10:00 @21:17:54.49 Waiting for nadia to answer "Move INTAKE line to MillQ water" @21:17:54.53 Answered Move INTAKE line to MillQ water"" @21:17:54.56 Toroid.seek :bypassed @21:17:54.74 Priming sample loop w/50ml, bypass w/25ml @21:17:54.76 SamplePump.setPosition! 0ml @21:17:54.86 Bypass.open @21:17:56.59 Intake.open @21:17:58.30 Abs.in :default @21:17:58.39 Abs.hold @21:17:58.66 SamplePump.in :start @21:17:58.79 SamplePump.seek 25ml,2:10 @21:17:59.34 SamplePump.configure :prime @21:18:28.83 Abs.stop @21:18:28.91 SamplePump.setPosition! 0ml @21:18:28.99 Exhaust.open @21:18:30.54 Bypass.close @21:18:32.21 Abs.in :default @21:18:32.30 Abs.hold @21:18:32.62 SamplePump.in :start @21:18:32.76 SamplePump.seek 50ml,3:50 @21:18:33.31 SamplePump.configure :prime @21:19:32.40 Abs.stop @21:19:32.48 Toroid.seek :cartridge @21:19:36.87 Sampling 25.0ml @21:19:36.89 SamplePump.setPosition! 0ml @21:19:37.16 Abs.hold @21:19:37.53 SamplePump.in :start @21:19:37.60 SamplePump.seek 25ml,2:35 @21:19:38.22 SamplePump.configure :sample @21:20:07.65 Abs.stop @21:20:07.79 Intake.close @21:20:07.88 Exhaust.close @21:20:08.00 Toroid.seek :bypassed @21:20:12.38 Sampled 25.0ml @21:20:12.71 Air.in :unlocking @21:20:12.86 Air.seek :clear @21:20:14.53 Lysis1.in :unlocking @21:20:14.73 Lysis1.seek :clear @21:20:14.85 Plunge.in :unlocking @21:20:14.95 Plunge.seek :clear @21:20:16.03 Portable sample Path clean, ready for next sample @21:22:12.87 -> BACsampler.goalVolume=1000 1000 @21:22:18.34 -> BACsampler.goalVolume 1000 @21:22:37.46 -> ESP.ready! @21:22:38.13 Toroid.seek :clear @21:22:40.45 Air.in :default @21:22:40.55 Air.seek :clear @21:22:40.71 Lysis1.in :default @21:22:40.80 Lysis1.seek :clear @21:22:40.92 Lysis2.in :default @21:22:41.00 Lysis2.seek :clear @21:22:41.11 Archive.in :default @21:22:41.20 Archive.seek :clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 25ml Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Abs = Absolute Pressure (Leak) Sensor @14psia Toroid = Toroid Valve (TV) clear PinPort = Handoff Pin Port (PP) clear Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear @21:22:44.32 -> Cart.fire @21:22:44.44 Lysis1.in :fast @21:22:44.53 Lysis1.seek :locked @21:22:47.10 Plunge.in :default @21:22:47.20 Plunge.seek :locked @21:22:47.39 Air.in :fast @21:22:47.47 Air.seek :locked @21:22:48.46 Powering cartridge bus @21:22:51.15 PuckHeater.configure :default #ambient 23.1C @21:22:51.24 Gate.power :heat,:ON,limit to 5000mA @21:22:51.94 Mounted lysis Cartridge board#J59944 @21:22:51.99 Using default cartridge heat loss curve for HeatingFunctionInStage @21:22:52.26 Air.in :default @21:22:52.34 Toroid.seek :bypassed @21:22:52.39 Air.seek :sealed @21:22:54.68 Priming sample loop w/25ml, bypass w/10ml @21:22:54.73 SamplePump.setPosition! 0ml @21:22:54.81 Bypass.open @21:22:56.44 Intake.open @21:22:57.42 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:23:00.05 Vacuum tare is 53% of full scale at 23C (drifted 1%) @21:23:00.27 Abs.in :default @21:23:00.35 Abs.hold @21:23:00.62 SamplePump.in :start @21:23:00.76 SamplePump.seek 10ml,1:10 @21:23:01.31 SamplePump.configure :prime @21:23:13.02 Abs.stop @21:23:13.11 SamplePump.setPosition! 0ml @21:23:13.19 Exhaust.open @21:23:14.76 Bypass.close @21:23:16.48 Abs.in :default @21:23:16.57 Abs.hold @21:23:16.95 SamplePump.in :start @21:23:17.04 SamplePump.seek 25ml,2:10 @21:23:17.64 SamplePump.configure :prime @21:23:47.07 Abs.stop @21:23:47.14 Bypass.open @21:23:48.03 Intake.close @21:23:48.89 Toroid.seek :crack @21:23:53.17 Toroid.seek :cartridge @21:23:54.29 Pressurizing cartridge to 26psi above 12.7psia @21:23:54.31 SamplePump.setPosition! 0ml @21:23:54.40 Intake.open @21:23:55.29 Bypass.close @21:23:56.16 Exhaust.close @21:23:57.16 Abs.in :default @21:23:57.24 Abs.hold @21:23:57.50 SamplePump.in :charge @21:23:57.59 SamplePump.seek 2.3ml,40 seconds @21:24:13.58 Abs.stop @21:24:13.66 Intake.close @21:24:14.61 Pressurized cartridge with 1.178ml to 26.3psi @21:24:14.63 Isolated @38.4psia (25.7psi) @21:24:14.70 Verifying cartridge holds at least 0.65..0.85 of 38.4psia @21:24:14.77 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @21:24:19.39 Cartridge pressure fell 2% to 37.5psia @21:24:19.42 Toroid.seek :cartridge @21:24:19.57 Sampling 1000.0ml @21:24:19.59 SamplePump.setPosition! 0ml @21:24:19.69 Exhaust.open @21:24:20.56 Intake.open @21:24:21.63 Abs.in :default @21:24:21.70 Abs.hold @21:24:22.05 SamplePump.in :start @21:24:22.15 SamplePump.seek 1000ml,1:07:10 @21:24:22.74 SamplePump.configure :sample @21:24:25.13 filtering 2/1000ml at 0.81ml/s with 22.7psi vacuum @21:25:25.19 filtering 52/1000ml at 0.83ml/s with 22.2psi vacuum @21:26:25.20 filtering 102/1000ml at 0.84ml/s with 24.4psi vacuum @21:27:25.19 filtering 152/1000ml at 0.84ml/s with 21.7psi vacuum @21:28:25.20 filtering 202/1000ml at 0.83ml/s with 23.3psi vacuum @21:29:25.19 filtering 252/1000ml at 0.83ml/s with 22.5psi vacuum @21:30:25.20 filtering 302/1000ml at 0.83ml/s with 18.4psi vacuum @21:31:25.19 filtering 352/1000ml at 0.83ml/s with 23.9psi vacuum @21:32:25.20 filtering 402/1000ml at 0.83ml/s with 22.9psi vacuum @21:33:25.21 filtering 451/1000ml at 0.82ml/s with 18.5psi vacuum @21:34:25.19 filtering 500/1000ml at 0.82ml/s with 20.7psi vacuum @21:35:25.19 filtering 550/1000ml at 0.82ml/s with 23.1psi vacuum @21:36:25.20 filtering 599/1000ml at 0.82ml/s with 21.1psi vacuum @21:37:25.19 filtering 648/1000ml at 0.82ml/s with 19.2psi vacuum @21:38:25.21 filtering 697/1000ml at 0.82ml/s with 23.7psi vacuum @21:39:25.20 filtering 746/1000ml at 0.82ml/s with 19.6psi vacuum @21:40:25.20 filtering 795/1000ml at 0.82ml/s with 22.3psi vacuum @21:41:25.19 filtering 844/1000ml at 0.81ml/s with 19.4psi vacuum @21:42:25.19 filtering 893/1000ml at 0.81ml/s with 22.0psi vacuum @21:43:25.19 filtering 941/1000ml at 0.81ml/s with 14.7psi vacuum @21:44:25.20 filtering 990/1000ml at 0.81ml/s with 23.2psi vacuum @21:44:37.35 Abs.stop @21:44:37.45 Intake.close @21:44:38.22 Exhaust.close @21:44:38.33 Toroid.seek :bypassed @21:44:42.74 Sampled 1000.0ml @21:44:42.80 Toroid.seek :bypassed @21:44:42.95 delay 5 seconds @21:44:47.97 Handoff.select :waste @21:44:48.14 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @21:44:54.79 Syringe tare is 51% of full scale at 23C (drifted 1%) @21:44:54.82 PinPort.in :locking @21:44:54.93 PinPort.seek :locked @21:44:55.36 Archive.in :fast @21:44:55.43 Lysis2.in :fast @21:44:55.50 Lysis1.seek 1250uL @21:44:55.66 Archive.seek :empty @21:44:55.83 Lysis2.seek :empty @21:45:04.19 Evacuating puck with filter bubble point of 50psi @21:45:04.23 PinPort.seek :locked @21:45:04.36 Handoff.select :waste @21:45:04.42 Plunge.seek :puckBottom @21:45:05.81 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @21:45:12.41 Air syringe pumping up to 1 strokes to reach 8psi @21:45:12.42 Air stroke #1 of 1 @21:45:12.57 Air.in :pressurize @21:45:12.65 Air.seek :pressurized @21:45:18.06 0.2psi after pressurize @21:45:18.08 Air.in :compress @21:45:18.19 Air.seek :evac @21:45:25.79 2.5psi after compress @21:45:38.89 0.1psi after compress (stable) @21:45:38.91 Air.in :default @21:45:39.02 Air.hold @21:45:39.29 at 0.1psi Opened to waste @21:45:39.31 Handoff.select :blocked @21:45:39.49 Plunge.seek :puckTop @21:45:40.41 Air syringe pumping up to 8 strokes to reach 50psi @21:45:40.42 Air stroke #1 of 8 @21:45:40.56 Air.in :compress @21:45:40.64 Air.seek :evac @21:45:40.87 0.1psi after compress @21:45:51.46 0.2psi after compress (stable) @21:45:51.49 Air.in :gulp @21:45:51.60 Air.seek :topVent @21:45:58.25 0.1psi after gulp @21:46:03.84 0.1psi after gulp (stable) @21:46:03.85 Air stroke #2 of 8 @21:46:03.99 Air.in :pressurize @21:46:04.10 Air.seek :pressurized @21:46:10.45 0.6psi after pressurize @21:46:10.48 Air.in :compress @21:46:10.61 Air.seek :evac @21:46:18.23 15.8psi after compress @21:46:28.82 17.3psi after compress (stable) @21:46:28.85 Air.in :gulp @21:46:28.96 Air.seek :topVent @21:46:35.60 17.1psi after gulp @21:46:41.20 17.1psi after gulp (stable) @21:46:41.28 Air stroke #3 of 8 @21:46:41.43 Air.in :pressurize @21:46:41.52 Air.seek :pressurized @21:46:47.89 16.9psi after pressurize @21:46:47.92 Air.in :compress @21:46:48.02 Air.seek :evac @21:46:55.63 31.3psi after compress @21:47:06.22 32.8psi after compress (stable) @21:47:06.25 Air.in :gulp @21:47:06.36 Air.seek :topVent @21:47:12.99 32.4psi after gulp @21:47:22.58 31.8psi after gulp (stable) @21:47:22.71 Air stroke #4 of 8 @21:47:22.86 Air.in :pressurize @21:47:22.96 Air.seek :pressurized @21:47:29.34 31.6psi after pressurize @21:47:29.37 Air.in :compress @21:47:29.48 Air.seek :evac @21:47:37.08 45.0psi after compress @21:47:47.67 46.7psi after compress (stable) @21:47:47.70 Air.in :gulp @21:47:47.81 Air.seek :topVent @21:47:54.47 46.2psi after gulp @21:48:00.20 46.2psi after gulp (stable) @21:48:00.31 Air stroke #5 of 8 @21:48:00.47 Air.in :pressurize @21:48:00.56 Air.seek :pressurized @21:48:06.93 46.1psi after pressurize @21:48:06.96 Air.in :compress @21:48:07.09 Air.seek :evac @21:48:12.96 Reached 50.7 of 50.0psi after pumping 8952uL (4.94 strokes) @21:48:12.98 Air.in :default @21:48:13.09 Air.hold @21:48:13.20 Plunge.seek :puckBottom @21:48:14.10 delay 3 seconds @21:48:17.12 Handoff.select :waste @21:48:17.29 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @21:48:26.38 delay 1:00 @21:49:26.41 Handoff.select :blocked @21:49:26.56 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @21:49:56.16 Handoff.select :waste @21:49:56.32 5.9psi at evacEnd @21:49:56.33 Handoff.select :blocked @21:49:56.52 Plunge.seek :puckTop @21:49:57.43 Air.in :fast @21:49:57.51 Air.seek :topVent @21:50:03.83 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @21:50:10.59 Stable @23.5psi after Handoff blocked @21:50:10.67 Air syringe pumping up to 8 strokes to reach 35psi @21:50:10.74 Air stroke #1 of 8 @21:50:10.89 Air.in :pressurize @21:50:10.97 Air.seek :pressurized @21:50:17.33 23.2psi after pressurize @21:50:17.36 Air.in :compress @21:50:17.49 Air.seek :evac @21:50:24.81 Reached 35.6 of 35.0psi after pumping 1791uL (0.99 strokes) @21:50:24.84 Air.in :default @21:50:24.94 Air.hold @21:50:25.05 Plunge.seek :puckBottom @21:50:25.97 delay 3 seconds @21:50:28.99 Handoff.select :waste @21:50:29.15 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @21:50:35.77 delay 1:00 @21:51:35.80 Handoff.select :blocked @21:51:35.97 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @21:51:58.56 Handoff.select :waste @21:51:58.72 0.1psi at evacEnd @21:51:58.75 Plunge.seek :pTop @21:51:59.68 delay 5 seconds @21:52:04.72 Air.in :glide @21:52:04.80 Air.seek :fullVent @21:52:06.38 delay 2 seconds @21:52:08.41 Plunge.seek :pBottom @21:52:09.29 Seawater evacuated, Pressure above/below filter released. @21:52:09.32 Plunge.seek :locked @21:52:10.73 Handoff.select :waste @21:52:10.86 Lysis1.in :slow @21:52:10.94 Lysis1.seek 950uL @21:52:25.02 delay 5 seconds @21:52:30.04 Handoff.select :blocked @21:52:30.21 Plunge.seek :puckTop @21:52:31.24 Lysis1.seek 350uL @21:52:59.22 delay 3 seconds @21:53:02.32 Air.in mix with 5.31227 parts Lysis1 @21:53:03.25 Air.seek :evac @21:53:03.30 Lysis1.seek 190uL @21:53:11.01 Air.in :fast @21:53:11.11 Air.seek :full @21:53:17.80 Lysis1.seek :empty @21:53:26.93 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @16.3psi @21:53:26.96 Plunge.seek :puckBottom @21:53:28.03 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @13.6psi @21:53:28.05 Polling Can every 2:00 @21:53:28.63 PuckHeater.seek 70.4C @21:54:38.03 PuckHeater.hold 2:00,70.4C @21:56:38.00 Polling Can every 2:00 @21:56:38.55 PuckHeater.configure :default #ambient 23.0C @21:56:38.64 PuckHeater.seek 69.3C @21:56:38.88 PuckHeater.hold 2:00,69.3C @21:58:38.87 Polling Can every 2:00 @21:58:39.42 PuckHeater.configure :default #ambient 23.0C @21:58:39.51 PuckHeater.hold 18:00,68.3C @22:16:37.53 Polling Can every 2:00 @22:16:38.08 PuckHeater.configure :default #ambient 22.8C @22:16:38.16 PuckHeater.seek 104.3C @22:17:42.10 PuckHeater.hold 2:00,104.3C @22:19:42.02 Polling Can every 2:00 @22:19:42.57 PuckHeater.configure :default #ambient 22.9C @22:19:42.67 PuckHeater.seek 103.2C @22:19:42.89 PuckHeater.hold 2:00,103.2C @22:21:42.77 Polling Can every 2:00 @22:21:43.32 PuckHeater.configure :default #ambient 22.8C @22:21:43.41 PuckHeater.hold 2:00,102.2C @22:23:43.37 delay 5:00 @22:28:43.45 Lysate cooked @4.5psi @22:28:43.48 Plunge.seek :puckTop @22:28:44.41 Air syringe pumping up to 5 strokes to reach 40psi @22:28:44.43 Air stroke #1 of 5 @22:28:44.57 Air.in :pressurize @22:28:44.65 Air.seek :pressurized @22:28:51.06 5.8psi after pressurize @22:28:51.09 Air.in :compress @22:28:51.20 Air.seek :evac @22:28:55.58 Reached 41.5 of 40.0psi after pumping 1586uL (0.88 strokes) @22:28:55.61 Air.in :default @22:28:55.71 Air.hold @22:28:55.82 delay 2 seconds @22:28:57.86 Air.in :fast @22:28:57.94 Air.seek :full @22:29:03.83 Plunge.seek :puckBottom @22:29:04.82 Lysis1.seek 138uL @22:29:11.40 delay 2 seconds @22:29:13.43 Plunge.seek :puckTop @22:29:14.34 Lysis1.seek :empty @22:29:20.94 Plunge.seek :puckBottom @22:29:22.02 Lysis1.in :extract @22:29:22.12 Lysis1.seek 675uL,nil @22:29:24.02 Air.in :pressurize @22:29:24.10 Air.seek :pressurized @22:29:24.36 Syringe::LysatePlungerStuck in nadia -- at < 206uL during Lysis Syringe #1 (LyS1) move @22:29:24.41 Lysis1.in :default @22:29:24.53 Lysis1.seek 176uL @22:29:30.46 13.9psi after pressurize @22:29:30.49 Air.in :compressLysate @22:29:30.59 Air.seek :evac @22:29:32.91 Reached 16.0 of 15.5psi after pumping 1419uL (0.78 strokes) @22:29:32.94 Air.in :default @22:29:33.03 Air.hold @22:29:33.19 Lysis1.in :extract @22:29:33.32 Lysis1.seek 675uL,nil @22:29:35.40 Air.in :compressLysate @22:29:35.52 Air.seek :evac @22:29:37.88 Reached 15.6 of 15.5psi after pumping 1448uL (0.80 strokes) @22:29:37.90 Air.in :default @22:29:37.99 Air.hold @22:29:38.93 Air.in :compressLysate @22:29:39.02 Air.seek :evac @22:29:50.72 Air.in :default @22:29:50.87 Air.hold @22:29:51.18 Pulled 675uL lysate @15.1psi @22:29:51.39 Lysis1.seek 800uL,nil @22:29:51.65 Air.in :compressLysate @22:29:51.77 Air.seek :evac @22:30:05.66 13.6psi after compress @22:30:16.33 13.9psi after compress (stable) @22:30:21.72 Air.in :default @22:30:21.80 Air.hold @22:30:21.89 Lysis1.in :default @22:30:22.00 Lysis1.hold @22:30:22.24 Air.in :glide @22:30:22.33 Air.seek :fullVent @22:30:23.56 Handoff.select :waste @22:30:23.72 Recovered 734uL lysate @14.0psi @22:30:23.74 delay 3 seconds @22:30:26.83 After 3 delay Recovered 734uL lysate @0.1psi @22:30:26.85 delay 5 seconds @22:30:31.92 Gate.power :heat,:OFF @22:30:31.95 Gate.power :cartridge,:OFF @22:30:32.09 PinPort.in :unlocking @22:30:32.16 PinPort.seek :clear @22:30:32.66 Archive.in :unlocking @22:30:32.87 Archive.seek :clear @22:30:33.02 Air.in :unlocking @22:30:33.18 Air.seek :clear @22:30:33.32 Lysis2.in :unlocking @22:30:33.40 Lysis2.seek :clear @22:30:38.84 Lysis1.in :unlocking @22:30:38.97 Lysis1.seek :clear @22:30:39.16 Plunge.in :unlocking @22:30:39.25 Plunge.seek :clear @02:59:57.14PDT09-Sep-25 -> BACsampler.goalVolume 1000 @03:00:00.58 -> Cart.fire @03:00:00.74 Lysis1.in :fast @03:00:00.82 Lysis1.seek :locked @03:00:03.37 Plunge.in :default @03:00:03.44 Plunge.seek :locked @03:00:03.64 Air.in :fast @03:00:03.72 Air.seek :locked @03:00:04.75 Powering cartridge bus @03:00:07.43 PuckHeater.configure :default #ambient 22.8C @03:00:07.53 Gate.power :heat,:ON,limit to 5000mA @03:00:08.27 Mounted lysis Cartridge board#J59963 @03:00:08.33 Using default cartridge heat loss curve for HeatingFunctionInStage @03:00:08.59 Air.in :default @03:00:08.63 Toroid.seek :bypassed @03:00:08.81 Air.seek :sealed @03:00:08.96 Priming sample loop w/25ml, bypass w/10ml @03:00:08.98 SamplePump.setPosition! 0ml @03:00:09.07 Bypass.open @03:00:10.75 Intake.open @03:00:11.76 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @03:00:14.38 Vacuum tare is 53% of full scale at 22C (drifted 1%) @03:00:14.56 Abs.in :default @03:00:14.64 Abs.hold @03:00:15.64 SamplePump.in :start @03:00:15.74 SamplePump.seek 10ml,1:10 @03:00:16.33 SamplePump.configure :prime @03:00:28.01 Abs.stop @03:00:28.09 SamplePump.setPosition! 0ml @03:00:28.18 Exhaust.open @03:00:29.74 Bypass.close @03:00:31.46 Abs.in :default @03:00:31.53 Abs.hold @03:00:31.87 SamplePump.in :start @03:00:31.96 SamplePump.seek 25ml,2:10 @03:00:32.56 SamplePump.configure :prime @03:01:01.99 Abs.stop @03:01:02.06 Bypass.open @03:01:02.94 Intake.close @03:01:03.83 Toroid.seek :crack @03:01:08.13 Toroid.seek :cartridge @03:01:09.25 Pressurizing cartridge to 26psi above 13.8psia @03:01:09.27 SamplePump.setPosition! 0ml @03:01:09.41 Intake.open @03:01:10.28 Bypass.close @03:01:11.13 Exhaust.close @03:01:12.12 Abs.in :default @03:01:12.20 Abs.hold @03:01:12.47 SamplePump.in :charge @03:01:12.56 SamplePump.seek 2.3ml,40 seconds @03:01:28.81 Abs.stop @03:01:28.89 Intake.close @03:01:29.84 Pressurized cartridge with 1.211ml to 26.5psi @03:01:29.86 Isolated @40.3psia (26.5psi) @03:01:29.96 Verifying cartridge holds at least 0.65..0.85 of 40.3psia @03:01:30.02 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @03:01:34.66 Cartridge pressure fell 1% to 40.0psia @03:01:34.72 Toroid.seek :cartridge @03:01:35.55 Sampling 1000.0ml @03:01:35.57 SamplePump.setPosition! 0ml @03:01:35.67 Exhaust.open @03:01:36.52 Intake.open @03:01:37.63 Abs.in :default @03:01:37.71 Abs.hold @03:01:38.08 SamplePump.in :start @03:01:38.18 SamplePump.seek 1000ml,1:07:10 @03:01:38.77 SamplePump.configure :sample @03:01:41.17 filtering 2/1000ml at 0.84ml/s with 20.3psi vacuum @03:02:41.22 filtering 51/1000ml at 0.81ml/s with 25.7psi vacuum @03:03:41.22 filtering 97/1000ml at 0.78ml/s with 28.6psi vacuum @03:04:41.22 filtering 142/1000ml at 0.75ml/s with 25.0psi vacuum @03:05:41.20 filtering 186/1000ml at 0.73ml/s with 19.5psi vacuum @03:06:41.22 filtering 228/1000ml at 0.70ml/s with 23.2psi vacuum @03:07:41.22 filtering 268/1000ml at 0.67ml/s with 22.4psi vacuum @03:08:41.22 filtering 307/1000ml at 0.64ml/s with 25.7psi vacuum @03:09:41.22 filtering 344/1000ml at 0.62ml/s with 23.0psi vacuum @03:10:41.22 filtering 381/1000ml at 0.61ml/s with 25.4psi vacuum @03:11:41.24 filtering 417/1000ml at 0.59ml/s with 23.2psi vacuum @03:12:41.21 filtering 452/1000ml at 0.58ml/s with 16.0psi vacuum @03:13:41.22 filtering 486/1000ml at 0.57ml/s with 20.9psi vacuum @03:14:41.22 filtering 519/1000ml at 0.55ml/s with 24.0psi vacuum @03:15:41.23 filtering 551/1000ml at 0.54ml/s with 26.6psi vacuum @03:16:41.22 filtering 583/1000ml at 0.53ml/s with 23.5psi vacuum @03:17:41.23 filtering 614/1000ml at 0.52ml/s with 25.7psi vacuum @03:18:41.22 filtering 645/1000ml at 0.51ml/s with 20.2psi vacuum @03:19:41.20 filtering 675/1000ml at 0.50ml/s with 20.8psi vacuum @03:20:41.22 filtering 704/1000ml at 0.49ml/s with 24.8psi vacuum @03:21:41.22 filtering 733/1000ml at 0.48ml/s with 19.9psi vacuum @03:22:41.22 filtering 761/1000ml at 0.47ml/s with 24.2psi vacuum @03:23:41.22 filtering 789/1000ml at 0.46ml/s with 25.1psi vacuum @03:24:41.23 filtering 816/1000ml at 0.45ml/s with 24.3psi vacuum @03:25:41.23 filtering 843/1000ml at 0.45ml/s with 26.1psi vacuum @03:26:41.22 filtering 869/1000ml at 0.44ml/s with 25.0psi vacuum @03:27:41.23 filtering 895/1000ml at 0.43ml/s with 24.5psi vacuum @03:28:41.23 filtering 921/1000ml at 0.42ml/s with 24.3psi vacuum @03:29:41.22 filtering 946/1000ml at 0.42ml/s with 20.7psi vacuum @03:30:41.22 filtering 970/1000ml at 0.41ml/s with 19.3psi vacuum @03:31:41.22 filtering 995/1000ml at 0.41ml/s with 25.5psi vacuum @03:31:54.28 Abs.stop @03:31:54.40 Intake.close @03:31:54.49 Exhaust.close @03:31:54.58 Handoff.select :close @03:31:54.68 Toroid.seek :bypassed @03:31:59.16 Sampled 1000.0ml @03:31:59.22 Toroid.seek :bypassed @03:31:59.35 delay 5 seconds @03:32:04.38 Handoff.select :waste @03:32:04.55 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:32:11.17 Syringe tare is 52% of full scale at 22C (drifted 2%) @03:32:11.21 PinPort.in :locking @03:32:11.33 PinPort.seek :locked @03:32:11.75 Archive.in :fast @03:32:11.86 Lysis2.in :fast @03:32:11.92 Lysis1.seek 1250uL @03:32:12.09 Archive.seek :empty @03:32:12.16 Lysis2.seek :empty @03:32:20.58 Evacuating puck with filter bubble point of 50psi @03:32:20.62 PinPort.seek :locked @03:32:20.79 Handoff.select :waste @03:32:20.88 Plunge.seek :puckBottom @03:32:22.30 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:32:29.17 Air syringe pumping up to 1 strokes to reach 8psi @03:32:29.19 Air stroke #1 of 1 @03:32:29.35 Air.in :pressurize @03:32:29.44 Air.seek :pressurized @03:32:34.81 0.1psi after pressurize @03:32:34.84 Air.in :compress @03:32:34.97 Air.seek :evac @03:32:42.57 1.0psi after compress @03:32:53.16 0.1psi after compress (stable) @03:32:53.18 Air.in :default @03:32:53.28 Air.hold @03:32:53.52 at 0.2psi Opened to waste @03:32:53.54 Handoff.select :blocked @03:32:53.72 Plunge.seek :puckTop @03:32:54.63 Air syringe pumping up to 8 strokes to reach 50psi @03:32:54.64 Air stroke #1 of 8 @03:32:54.83 Air.in :compress @03:32:54.92 Air.seek :evac @03:32:55.10 12.5psi after compress @03:33:05.69 12.6psi after compress (stable) @03:33:05.73 Air.in :gulp @03:33:05.84 Air.seek :topVent @03:33:12.51 12.7psi after gulp @03:33:18.60 12.7psi after gulp (stable) @03:33:18.62 Air stroke #2 of 8 @03:33:18.81 Air.in :pressurize @03:33:18.90 Air.seek :pressurized @03:33:25.26 12.5psi after pressurize @03:33:25.29 Air.in :compress @03:33:25.39 Air.seek :evac @03:33:33.00 31.8psi after compress @03:33:44.26 33.9psi after compress (stable) @03:33:44.38 Air.in :gulp @03:33:44.50 Air.seek :topVent @03:33:51.14 34.0psi after gulp @03:33:56.77 33.8psi after gulp (stable) @03:33:56.79 Air stroke #3 of 8 @03:33:56.92 Air.in :pressurize @03:33:57.02 Air.seek :pressurized @03:34:03.38 33.8psi after pressurize @03:34:03.41 Air.in :compress @03:34:03.52 Air.seek :evac @03:34:11.13 Reached 50.8 of 50.0psi after pumping 5430uL (3.00 strokes) @03:34:11.15 Air.in :default @03:34:11.26 Air.hold @03:34:11.39 Plunge.seek :puckBottom @03:34:12.31 delay 3 seconds @03:34:15.33 Handoff.select :waste @03:34:15.51 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:34:26.12 delay 1:00 @03:35:26.15 Handoff.select :blocked @03:35:26.31 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @03:35:54.62 Handoff.select :waste @03:35:54.92 4.2psi at evacEnd @03:35:54.93 Handoff.select :blocked @03:35:55.11 Plunge.seek :puckTop @03:35:56.02 Air.in :fast @03:35:56.11 Air.seek :topVent @03:36:02.79 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @03:36:09.39 Stable @27.4psi after Handoff blocked @03:36:09.42 Air syringe pumping up to 8 strokes to reach 35psi @03:36:09.45 Air stroke #1 of 8 @03:36:09.60 Air.in :pressurize @03:36:09.68 Air.seek :pressurized @03:36:16.04 27.5psi after pressurize @03:36:16.08 Air.in :compress @03:36:16.18 Air.seek :evac @03:36:22.43 Reached 35.4 of 35.0psi after pumping 1732uL (0.96 strokes) @03:36:22.46 Air.in :default @03:36:22.56 Air.hold @03:36:22.71 Plunge.seek :puckBottom @03:36:23.62 delay 3 seconds @03:36:26.65 Handoff.select :waste @03:36:26.85 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:36:36.59 delay 1:00 @03:37:36.65 Handoff.select :blocked @03:37:36.86 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @03:37:59.45 Handoff.select :waste @03:37:59.61 1.0psi at evacEnd @03:37:59.64 Plunge.seek :pTop @03:38:00.57 delay 5 seconds @03:38:05.60 Air.in :glide @03:38:05.67 Air.seek :fullVent @03:38:07.95 delay 2 seconds @03:38:09.98 Plunge.seek :pBottom @03:38:11.60 Seawater evacuated, Pressure above/below filter released. @03:38:11.63 Plunge.seek :locked @03:38:13.04 Handoff.select :waste @03:38:13.17 Lysis1.in :slow @03:38:13.25 Lysis1.seek 950uL @03:38:27.32 delay 5 seconds @03:38:32.36 Handoff.select :blocked @03:38:32.52 Plunge.seek :puckTop @03:38:33.56 Lysis1.seek 350uL @03:39:01.56 delay 3 seconds @03:39:04.67 Air.in mix with 5.31227 parts Lysis1 @03:39:05.49 Air.seek :evac @03:39:05.61 Lysis1.seek 190uL @03:39:13.29 Air.in :fast @03:39:13.40 Air.seek :full @03:39:20.09 Lysis1.seek :empty @03:39:29.24 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @14.4psi @03:39:29.27 Plunge.seek :puckBottom @03:39:30.34 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @12.7psi @03:39:30.36 Polling Can every 2:00 @03:39:30.93 PuckHeater.configure :default #ambient 22.4C @03:39:31.02 PuckHeater.seek 70.4C @03:40:47.92 PuckHeater.hold 2:00,70.4C @03:42:47.66 Polling Can every 2:00 @03:42:48.21 PuckHeater.configure :default #ambient 22.6C @03:42:48.30 PuckHeater.seek 69.3C @03:42:48.51 PuckHeater.hold 2:00,69.3C @03:44:48.30 Polling Can every 2:00 @03:44:48.85 PuckHeater.configure :default #ambient 22.5C @03:44:48.94 PuckHeater.hold 18:00,68.3C @04:02:45.82 Polling Can every 2:00 @04:02:46.38 PuckHeater.configure :default #ambient 22.4C @04:02:46.46 PuckHeater.seek 104.3C @04:03:52.47 PuckHeater.hold 2:00,104.3C @04:05:52.20 Polling Can every 2:00 @04:05:52.75 PuckHeater.configure :default #ambient 22.5C @04:05:52.84 PuckHeater.seek 103.2C @04:05:53.03 PuckHeater.hold 2:00,103.2C @04:07:52.79 Polling Can every 2:00 @04:07:53.34 PuckHeater.configure :default #ambient 22.6C @04:07:53.44 PuckHeater.hold 2:00,102.2C @04:09:54.01 delay 5:00 @04:14:54.10 Lysate cooked @6.9psi @04:14:54.12 Plunge.seek :puckTop @04:14:55.07 Air syringe pumping up to 5 strokes to reach 40psi @04:14:55.09 Air stroke #1 of 5 @04:14:55.22 Air.in :pressurize @04:14:55.31 Air.seek :pressurized @04:15:01.67 7.5psi after pressurize @04:15:01.70 Air.in :compress @04:15:01.82 Air.seek :evac @04:15:06.82 Reached 41.5 of 40.0psi after pumping 1658uL (0.92 strokes) @04:15:06.85 Air.in :default @04:15:06.94 Air.hold @04:15:07.06 delay 2 seconds @04:15:09.09 Air.in :fast @04:15:09.18 Air.seek :full @04:15:15.32 Plunge.seek :puckBottom @04:15:16.31 Lysis1.seek 138uL @04:15:22.89 delay 2 seconds @04:15:24.93 Plunge.seek :puckTop @04:15:25.83 Lysis1.seek :empty @04:15:32.43 Plunge.seek :puckBottom @04:15:33.49 Lysis1.in :extract @04:15:33.56 Lysis1.seek 675uL,nil @04:15:36.13 Air.in :pressurize @04:15:36.22 Air.seek :pressurized @04:15:42.62 11.0psi after pressurize @04:15:42.65 Air.in :compressLysate @04:15:42.79 Air.seek :evac @04:16:05.23 Air.in :default @04:16:05.34 Air.hold @04:16:05.63 Pulled 675uL lysate @15.6psi @04:16:05.86 Lysis1.seek 800uL,nil @04:16:07.24 Air.in :compressLysate @04:16:07.32 Air.seek :evac @04:16:14.60 13.2psi after compress @04:16:25.27 13.8psi after compress (stable) @04:16:30.47 Air.in :default @04:16:30.54 Air.hold @04:16:30.63 Lysis1.in :default @04:16:30.80 Lysis1.hold @04:16:31.04 Air.in :glide @04:16:31.13 Air.seek :fullVent @04:16:32.36 Handoff.select :waste @04:16:32.51 Recovered 715uL lysate @14.0psi @04:16:32.54 delay 3 seconds @04:16:35.63 After 3 delay Recovered 715uL lysate @-1.0psi @04:16:35.65 delay 5 seconds @04:16:40.72 Gate.power :heat,:OFF @04:16:40.75 Gate.power :cartridge,:OFF @04:16:40.90 PinPort.in :unlocking @04:16:40.98 PinPort.seek :clear @04:16:42.15 Archive.in :unlocking @04:16:42.29 Archive.seek :clear @04:16:42.43 Air.in :unlocking @04:16:42.58 Air.seek :clear @04:16:42.74 Lysis2.in :unlocking @04:16:42.83 Lysis2.seek :clear @04:16:47.57 Lysis1.in :unlocking @04:16:47.73 Lysis1.seek :clear @04:16:47.86 Plunge.in :unlocking @04:16:47.95 Plunge.seek :clear @04:17:10.37 -> cleanPortable @04:17:10.88 Waiting for nadia to answer "LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue" @04:17:12.58 Answered LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue"" @04:17:12.74 Lysis1.in :fast @04:17:12.82 Lysis1.seek :locked @04:17:15.38 Plunge.in :default @04:17:15.51 Plunge.seek :locked @04:17:15.67 Air.in :fast @04:17:15.74 Air.seek :locked @04:17:16.76 Powering cartridge bus @04:17:19.44 Gate.power :cartridge,:OFF @04:17:20.01 Mounted archive Cartridge @04:17:20.06 Toroid.seek :bypassed @04:17:20.17 Priming sample loop w/10ml, bypass w/10ml @04:17:20.19 SamplePump.setPosition! 0ml @04:17:20.28 Bypass.open @04:17:21.90 Intake.open @04:17:22.87 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @04:17:25.49 Vacuum tare is 54% of full scale at 23C (drifted 2%) @04:17:25.68 Abs.in :default @04:17:25.77 Abs.hold @04:17:26.04 SamplePump.in :start @04:17:26.13 SamplePump.seek 10ml,1:10 @04:17:26.73 SamplePump.configure :prime @04:17:38.41 Abs.stop @04:17:38.49 SamplePump.setPosition! 0ml @04:17:38.56 Exhaust.open @04:17:40.13 Bypass.close @04:17:42.54 Abs.in :default @04:17:42.62 Abs.hold @04:17:42.99 SamplePump.in :start @04:17:43.09 SamplePump.seek 10ml,1:10 @04:17:43.68 SamplePump.configure :prime @04:17:55.36 Abs.stop @04:17:55.46 Waiting for nadia to answer "INTAKE line plumbed to 10% Bleach, push return to continue" @04:18:17.40 Answered INTAKE line plumbed to 10% Bleach, push return to continue"" @04:18:17.43 Toroid.seek :bypassed @04:18:17.56 Priming sample loop w/10ml, bypass w/10ml @04:18:17.59 SamplePump.setPosition! 0ml @04:18:17.67 Exhaust.close @04:18:18.52 Bypass.open @04:18:20.31 Abs.in :default @04:18:20.40 Abs.hold @04:18:20.69 SamplePump.in :start @04:18:20.81 SamplePump.seek 10ml,1:10 @04:18:21.36 SamplePump.configure :prime @04:18:33.07 Abs.stop @04:18:33.17 SamplePump.setPosition! 0ml @04:18:33.26 Exhaust.open @04:18:34.77 Bypass.close @04:18:36.55 Abs.in :default @04:18:36.64 Abs.hold @04:18:37.02 SamplePump.in :start @04:18:37.11 SamplePump.seek 10ml,1:10 @04:18:37.71 SamplePump.configure :prime @04:18:49.38 Abs.stop @04:18:49.46 Toroid.seek :cartridge @04:18:53.81 Sampling 25.0ml @04:18:53.84 SamplePump.setPosition! 0ml @04:18:54.10 Abs.in :default @04:18:54.18 Abs.hold @04:18:54.52 SamplePump.in :start @04:18:54.62 SamplePump.seek 25ml,2:10 @04:18:55.21 SamplePump.configure :sample @04:19:24.72 Abs.stop @04:19:24.80 Intake.close @04:19:24.93 Exhaust.close @04:19:25.05 Handoff.select :close @04:19:25.14 Toroid.seek :bypassed @04:19:29.53 Sampled 25.0ml @04:19:29.56 delay 10:00 @04:29:29.60 Waiting for nadia to answer "Move INTAKE line to MillQ water" @04:29:29.65 Answered Move INTAKE line to MillQ water"" @04:29:29.68 Toroid.seek :bypassed @04:29:29.81 Priming sample loop w/50ml, bypass w/25ml @04:29:29.83 SamplePump.setPosition! 0ml @04:29:29.93 Bypass.open @04:29:31.66 Intake.open @04:29:33.35 Abs.in :default @04:29:33.44 Abs.hold @04:29:33.71 SamplePump.in :start @04:29:33.81 SamplePump.seek 25ml,2:10 @04:29:34.40 SamplePump.configure :prime @04:30:03.82 Abs.stop @04:30:03.91 SamplePump.setPosition! 0ml @04:30:04.00 Exhaust.open @04:30:05.54 Bypass.close @04:30:07.24 Abs.in :default @04:30:07.32 Abs.hold @04:30:07.65 SamplePump.in :start @04:30:07.73 SamplePump.seek 50ml,3:50 @04:30:08.34 SamplePump.configure :prime @04:31:07.34 Abs.stop @04:31:07.42 Toroid.seek :cartridge @04:31:11.79 Sampling 25.0ml @04:31:11.82 SamplePump.setPosition! 0ml @04:31:12.09 Abs.in :default @04:31:12.18 Abs.hold @04:31:12.53 SamplePump.in :start @04:31:12.62 SamplePump.seek 25ml,2:35 @04:31:13.22 SamplePump.configure :sample @04:31:42.70 Abs.stop @04:31:42.79 Intake.close @04:31:42.90 Exhaust.close @04:31:42.96 Toroid.seek :bypassed @04:31:47.40 Sampled 25.0ml @04:31:47.68 Air.in :unlocking @04:31:47.82 Air.seek :clear @04:31:48.83 Lysis1.in :unlocking @04:31:48.99 Lysis1.seek :clear @04:31:49.75 Plunge.in :unlocking @04:31:49.89 Plunge.seek :clear @04:31:51.02 Portable sample Path clean, ready for next sample @04:33:09.77 -> Cart.fire @04:33:09.89 Lysis1.in :fast @04:33:09.97 Lysis1.seek :locked @04:33:12.53 Plunge.in :default @04:33:12.63 Plunge.seek :locked @04:33:12.84 Air.in :fast @04:33:12.92 Air.seek :locked @04:33:13.91 Powering cartridge bus @04:33:16.59 PuckHeater.configure :default #ambient 22.6C @04:33:16.73 Gate.power :heat,:ON,limit to 5000mA @04:33:17.42 Mounted lysis Cartridge board#J59996 @04:33:17.47 Using default cartridge heat loss curve for HeatingFunctionInStage @04:33:17.75 Air.in :default @04:33:17.83 Toroid.seek :bypassed @04:33:17.88 Air.seek :sealed @04:33:18.07 Priming sample loop w/25ml, bypass w/10ml @04:33:18.09 SamplePump.setPosition! 0ml @04:33:18.19 Bypass.open @04:33:19.85 Intake.open @04:33:20.81 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @04:33:23.43 Vacuum tare is 54% of full scale at 23C (drifted 2%) @04:33:23.67 Abs.in :default @04:33:23.76 Abs.hold @04:33:24.03 SamplePump.in :start @04:33:24.13 SamplePump.seek 10ml,1:10 @04:33:24.72 SamplePump.configure :prime @04:33:36.40 Abs.stop @04:33:36.48 SamplePump.setPosition! 0ml @04:33:36.56 Exhaust.open @04:33:38.11 Bypass.close @04:33:39.78 Abs.in :default @04:33:39.86 Abs.hold @04:33:40.20 SamplePump.in :start @04:33:41.01 SamplePump.seek 25ml,2:10 @04:33:41.10 SamplePump.configure :prime @04:34:10.85 Abs.stop @04:34:10.93 Bypass.open @04:34:11.77 Intake.close @04:34:12.72 Toroid.seek :crack @04:34:17.02 Toroid.seek :cartridge @04:34:18.14 Pressurizing cartridge to 26psi above 13.0psia @04:34:18.17 SamplePump.setPosition! 0ml @04:34:18.26 Intake.open @04:34:19.23 Bypass.close @04:34:20.10 Exhaust.close @04:34:21.08 Abs.in :default @04:34:21.16 Abs.hold @04:34:21.41 SamplePump.in :charge @04:34:21.51 SamplePump.seek 2.3ml,40 seconds @04:34:37.72 Abs.stop @04:34:37.81 Intake.close @04:34:38.79 Pressurized cartridge with 1.209ml to 26.2psi @04:34:38.81 Isolated @40.2psia (27.2psi) @04:34:38.86 Verifying cartridge holds at least 0.65..0.85 of 40.2psia @04:34:38.92 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @04:34:43.54 Cartridge pressure fell 1% to 39.9psia @04:34:43.57 Toroid.seek :cartridge @04:34:43.72 Sampling 1000.0ml @04:34:43.74 SamplePump.setPosition! 0ml @04:34:43.84 Exhaust.open @04:34:44.73 Intake.open @04:34:45.78 Abs.in :default @04:34:45.87 Abs.hold @04:34:46.21 SamplePump.in :start @04:34:46.31 SamplePump.seek 1000ml,1:07:10 @04:34:46.90 SamplePump.configure :sample @04:34:49.31 filtering 2/1000ml at 0.84ml/s with 15.7psi vacuum @04:35:50.03 filtering 52/1000ml at 0.83ml/s with 25.6psi vacuum @04:36:49.36 filtering 100/1000ml at 0.81ml/s with 18.6psi vacuum @04:37:49.36 filtering 149/1000ml at 0.80ml/s with 22.6psi vacuum @04:38:49.36 filtering 196/1000ml at 0.78ml/s with 24.2psi vacuum @04:39:49.36 filtering 242/1000ml at 0.77ml/s with 26.6psi vacuum @04:40:49.36 filtering 287/1000ml at 0.75ml/s with 21.7psi vacuum @04:41:49.36 filtering 332/1000ml at 0.74ml/s with 20.0psi vacuum @04:42:49.36 filtering 376/1000ml at 0.73ml/s with 26.7psi vacuum @04:43:49.38 filtering 419/1000ml at 0.72ml/s with 21.9psi vacuum @04:44:49.36 filtering 461/1000ml at 0.70ml/s with 23.3psi vacuum @04:45:49.37 filtering 502/1000ml at 0.69ml/s with 25.8psi vacuum @04:46:49.37 filtering 543/1000ml at 0.68ml/s with 23.9psi vacuum @04:47:49.38 filtering 583/1000ml at 0.67ml/s with 25.1psi vacuum @04:48:49.36 filtering 623/1000ml at 0.66ml/s with 25.4psi vacuum @04:49:49.38 filtering 661/1000ml at 0.65ml/s with 24.4psi vacuum @04:50:49.36 filtering 700/1000ml at 0.64ml/s with 23.6psi vacuum @04:51:49.37 filtering 738/1000ml at 0.63ml/s with 25.4psi vacuum @04:52:49.36 filtering 775/1000ml at 0.62ml/s with 24.4psi vacuum @04:53:49.36 filtering 812/1000ml at 0.61ml/s with 21.9psi vacuum @04:54:49.36 filtering 848/1000ml at 0.61ml/s with 21.7psi vacuum @04:55:49.37 filtering 884/1000ml at 0.60ml/s with 22.7psi vacuum @04:56:49.36 filtering 920/1000ml at 0.60ml/s with 20.3psi vacuum @04:57:49.37 filtering 955/1000ml at 0.59ml/s with 23.6psi vacuum @04:58:49.36 filtering 990/1000ml at 0.58ml/s with 24.8psi vacuum @04:59:06.73 Abs.stop @04:59:06.83 Intake.close @04:59:06.91 Exhaust.close @04:59:07.05 Toroid.seek :bypassed @04:59:11.42 Sampled 1000.0ml @04:59:11.48 Toroid.seek :bypassed @04:59:11.59 delay 5 seconds @04:59:16.62 Handoff.select :waste @04:59:16.81 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @04:59:23.43 Syringe tare is 52% of full scale at 23C (drifted 1%) @04:59:23.48 PinPort.in :locking @04:59:23.59 PinPort.seek :locked @04:59:24.04 Archive.in :fast @04:59:24.10 Lysis2.in :fast @04:59:24.21 Lysis1.seek 1250uL @04:59:24.33 Archive.seek :empty @04:59:24.39 Lysis2.seek :empty @04:59:32.85 Evacuating puck with filter bubble point of 50psi @04:59:32.89 PinPort.seek :locked @04:59:33.02 Handoff.select :waste @04:59:33.06 Plunge.seek :puckBottom @04:59:34.47 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @04:59:41.07 Air syringe pumping up to 1 strokes to reach 8psi @04:59:41.09 Air stroke #1 of 1 @04:59:41.21 Air.in :pressurize @04:59:41.30 Air.seek :pressurized @04:59:46.68 0.2psi after pressurize @04:59:46.73 Air.in :compress @04:59:46.85 Air.seek :evac @04:59:54.46 0.3psi after compress @05:00:05.05 0.0psi after compress (stable) @05:00:05.09 Air.in :default @05:00:05.20 Air.hold @05:00:05.43 at 0.2psi Opened to waste @05:00:05.45 Handoff.select :blocked @05:00:05.63 Plunge.seek :puckTop @05:00:06.55 Air syringe pumping up to 8 strokes to reach 50psi @05:00:06.56 Air stroke #1 of 8 @05:00:06.74 Air.in :compress @05:00:06.83 Air.seek :evac @05:00:07.00 7.4psi after compress @05:00:17.60 7.7psi after compress (stable) @05:00:17.62 Air.in :gulp @05:00:17.74 Air.seek :topVent @05:00:24.39 7.6psi after gulp @05:00:29.99 7.7psi after gulp (stable) @05:00:30.01 Air stroke #2 of 8 @05:00:30.16 Air.in :pressurize @05:00:30.94 Air.seek :pressurized @05:00:37.32 7.9psi after pressurize @05:00:37.35 Air.in :compress @05:00:37.46 Air.seek :evac @05:00:45.08 24.7psi after compress @05:00:55.67 26.4psi after compress (stable) @05:00:55.70 Air.in :gulp @05:00:55.82 Air.seek :topVent @05:01:02.47 26.1psi after gulp @05:01:08.06 25.9psi after gulp (stable) @05:01:08.07 Air stroke #3 of 8 @05:01:08.21 Air.in :pressurize @05:01:08.28 Air.seek :pressurized @05:01:14.65 25.3psi after pressurize @05:01:14.70 Air.in :compress @05:01:14.83 Air.seek :evac @05:01:22.44 40.3psi after compress @05:01:33.05 41.6psi after compress (stable) @05:01:33.12 Air.in :gulp @05:01:33.24 Air.seek :topVent @05:01:39.90 40.8psi after gulp @05:01:52.01 39.7psi after gulp (stable) @05:01:52.02 Air stroke #4 of 8 @05:01:52.16 Air.in :pressurize @05:01:52.25 Air.seek :pressurized @05:01:58.61 38.9psi after pressurize @05:01:58.64 Air.in :compress @05:01:58.78 Air.seek :evac @05:02:06.69 Reached 51.9 of 50.0psi after pumping 7219uL (3.99 strokes) @05:02:06.73 Air.in :default @05:02:06.84 Air.hold @05:02:06.95 Plunge.seek :puckBottom @05:02:07.86 delay 3 seconds @05:02:10.89 Handoff.select :waste @05:02:11.04 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @05:02:29.14 delay 1:00 @05:03:29.17 Handoff.select :blocked @05:03:29.34 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @05:04:08.56 Pressure too High, open to waste again (8.1psi) @05:04:08.58 Handoff.select :waste @05:04:08.80 delay 1:00 @05:05:08.83 Handoff.select :blocked @05:05:09.00 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @05:05:37.09 Handoff.select :waste @05:05:37.28 3.5psi at evacEnd @05:05:37.30 Handoff.select :blocked @05:05:37.47 Plunge.seek :puckTop @05:05:38.38 Air.in :fast @05:05:38.47 Air.seek :topVent @05:05:45.07 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @05:05:51.66 Stable @16.7psi after Handoff blocked @05:05:51.69 Air syringe pumping up to 8 strokes to reach 35psi @05:05:51.73 Air stroke #1 of 8 @05:05:51.89 Air.in :pressurize @05:05:51.96 Air.seek :pressurized @05:05:58.32 16.8psi after pressurize @05:05:58.35 Air.in :compress @05:05:58.46 Air.seek :evac @05:06:06.79 29.6psi after compress @05:06:17.40 31.0psi after compress (stable) @05:06:17.43 Air.in :gulp @05:06:17.54 Air.seek :topVent @05:06:24.19 30.8psi after gulp @05:06:30.28 30.8psi after gulp (stable) @05:06:30.30 Air stroke #2 of 8 @05:06:30.44 Air.in :pressurize @05:06:30.53 Air.seek :pressurized @05:06:36.90 30.9psi after pressurize @05:06:36.93 Air.in :compress @05:06:37.04 Air.seek :evac @05:06:42.71 Reached 35.6 of 35.0psi after pumping 3507uL (1.94 strokes) @05:06:42.73 Air.in :default @05:06:42.83 Air.hold @05:06:42.94 Plunge.seek :puckBottom @05:06:43.85 delay 3 seconds @05:06:46.87 Handoff.select :waste @05:06:47.05 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @05:06:53.64 delay 1:00 @05:07:53.68 Handoff.select :blocked @05:07:53.85 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @05:08:26.44 Handoff.select :waste @05:08:26.62 4.3psi at evacEnd @05:08:26.65 Plunge.seek :pTop @05:08:27.60 delay 5 seconds @05:08:32.64 Air.in :glide @05:08:32.76 Air.seek :fullVent @05:08:35.40 delay 2 seconds @05:08:37.43 Plunge.seek :pBottom @05:08:38.34 Seawater evacuated, Pressure above/below filter released. @05:08:38.37 Plunge.seek :locked @05:08:39.76 Handoff.select :waste @05:08:39.90 Lysis1.in :slow @05:08:39.98 Lysis1.seek 950uL @05:08:54.07 delay 5 seconds @05:08:59.10 Handoff.select :blocked @05:08:59.28 Plunge.seek :puckTop @05:09:00.32 Lysis1.seek 350uL @05:09:28.33 delay 3 seconds @05:09:31.42 Air.in mix with 5.31227 parts Lysis1 @05:09:32.34 Air.seek :evac @05:09:32.43 Lysis1.seek 190uL @05:09:40.11 Air.in :fast @05:09:40.20 Air.seek :full @05:09:46.91 Lysis1.seek :empty @05:09:56.04 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @19.0psi @05:09:56.06 Plunge.seek :puckBottom @05:09:57.12 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @16.2psi @05:09:57.14 Polling Can every 2:00 @05:09:57.71 PuckHeater.configure :default #ambient 22.6C @05:09:57.79 PuckHeater.seek 70.4C @05:11:11.36 PuckHeater.hold 2:00,70.4C @05:13:11.48 Polling Can every 2:00 @05:13:12.04 PuckHeater.configure :default #ambient 22.6C @05:13:12.12 PuckHeater.seek 69.3C @05:13:12.33 PuckHeater.hold 2:00,69.3C @05:15:12.38 Polling Can every 2:00 @05:15:12.93 PuckHeater.configure :default #ambient 22.6C @05:15:13.02 PuckHeater.hold 18:00,68.3C @05:33:12.03 Polling Can every 2:00 @05:33:12.58 PuckHeater.configure :default #ambient 22.4C @05:33:12.67 PuckHeater.seek 104.3C @05:34:17.68 PuckHeater.hold 2:00,104.3C @05:36:17.76 Polling Can every 2:00 @05:36:18.31 PuckHeater.configure :default #ambient 22.4C @05:36:18.40 PuckHeater.seek 103.2C @05:36:18.61 PuckHeater.hold 2:00,103.2C @05:38:18.75 Polling Can every 2:00 @05:38:19.31 PuckHeater.configure :default #ambient 22.4C @05:38:19.40 PuckHeater.hold 2:00,102.2C @05:40:19.52 delay 5:00 @05:45:19.60 Lysate cooked @17.3psi @05:45:19.63 Plunge.seek :puckTop @05:45:21.31 Air syringe pumping up to 5 strokes to reach 40psi @05:45:21.33 Air stroke #1 of 5 @05:45:21.48 Air.in :pressurize @05:45:21.56 Air.seek :pressurized @05:45:27.92 17.2psi after pressurize @05:45:27.95 Air.in :compress @05:45:28.08 Air.seek :evac @05:45:33.30 Reached 41.3 of 40.0psi after pumping 1674uL (0.92 strokes) @05:45:33.32 Air.in :default @05:45:33.42 Air.hold @05:45:33.54 delay 2 seconds @05:45:35.58 Air.in :fast @05:45:35.67 Air.seek :full @05:45:41.87 Plunge.seek :puckBottom @05:45:42.84 Lysis1.seek 138uL @05:45:49.42 delay 2 seconds @05:45:51.45 Plunge.seek :puckTop @05:45:52.36 Lysis1.seek :empty @05:45:58.96 Plunge.seek :puckBottom @05:46:00.03 Lysis1.in :extract @05:46:00.15 Lysis1.seek 675uL,nil @05:46:02.53 Air.in :pressurize @05:46:02.65 Air.seek :pressurized @05:46:09.08 10.9psi after pressurize @05:46:09.13 Air.in :compressLysate @05:46:09.24 Air.seek :evac @05:46:32.34 Air.in :default @05:46:32.45 Air.hold @05:46:32.78 Pulled 675uL lysate @15.2psi @05:46:32.99 Lysis1.seek 800uL,nil @05:46:34.41 Air.in :compressLysate @05:46:34.51 Air.seek :evac @05:46:41.26 13.8psi after compress @05:46:52.18 14.6psi after compress (stable) @05:46:57.44 Air.in :default @05:46:57.51 Lysis1.in :default @05:46:57.55 Air.hold @05:46:57.72 Lysis1.hold @05:46:57.96 Air.in :glide @05:46:58.04 Air.seek :fullVent @05:46:59.28 Handoff.select :waste @05:46:59.43 Recovered 734uL lysate @14.7psi @05:46:59.45 delay 3 seconds @05:47:02.56 After 3 delay Recovered 734uL lysate @-1.3psi @05:47:02.58 delay 5 seconds @05:47:07.64 Gate.power :heat,:OFF @05:47:07.67 Gate.power :cartridge,:OFF @05:47:07.77 PinPort.in :unlocking @05:47:07.86 PinPort.seek :clear @05:47:08.36 Archive.in :unlocking @05:47:08.52 Archive.seek :clear @05:47:08.59 Air.in :unlocking @05:47:09.50 Air.seek :clear @05:47:09.72 Lysis2.in :unlocking @05:47:09.81 Lysis2.seek :clear @05:47:14.54 Lysis1.in :unlocking @05:47:14.75 Lysis1.seek :clear @05:47:14.84 Plunge.in :unlocking @05:47:14.97 Plunge.seek :clear @08:47:44.70 -> cleanPortable @08:47:45.18 Waiting for nadia to answer "LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue" @08:48:51.53 Answered LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue"" @08:48:51.64 Lysis1.in :fast @08:48:51.72 Lysis1.seek :locked @08:48:54.28 Plunge.in :default @08:48:54.35 Plunge.seek :locked @08:48:54.54 Air.in :fast @08:48:54.62 Air.seek :locked @08:48:55.64 Powering cartridge bus @08:48:58.31 Gate.power :cartridge,:OFF @08:48:58.87 Mounted archive Cartridge @08:48:58.93 Toroid.seek :bypassed @08:48:59.03 Priming sample loop w/10ml, bypass w/10ml @08:48:59.05 SamplePump.setPosition! 0ml @08:48:59.13 Bypass.open @08:49:00.79 Intake.open @08:49:01.78 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:49:04.40 Vacuum tare is 53% of full scale at 22C (drifted 1%) @08:49:04.59 Abs.in :default @08:49:04.70 Abs.hold @08:49:04.98 SamplePump.in :start @08:49:05.08 SamplePump.seek 10ml,1:10 @08:49:05.67 SamplePump.configure :prime @08:49:17.33 Abs.stop @08:49:17.41 SamplePump.setPosition! 0ml @08:49:17.49 Exhaust.open @08:49:19.05 Bypass.close @08:49:20.80 Abs.in :default @08:49:20.87 Abs.hold @08:49:21.21 SamplePump.in :start @08:49:21.31 SamplePump.seek 10ml,1:10 @08:49:21.90 SamplePump.configure :prime @08:49:33.57 Abs.stop @08:49:33.66 Waiting for nadia to answer "INTAKE line plumbed to 10% Bleach, push return to continue" @08:49:39.54 Answered INTAKE line plumbed to 10% Bleach, push return to continue"" @08:49:39.56 Toroid.seek :bypassed @08:49:39.69 Priming sample loop w/10ml, bypass w/10ml @08:49:39.71 SamplePump.setPosition! 0ml @08:49:39.80 Exhaust.close @08:49:40.65 Bypass.open @08:49:42.48 Abs.in :default @08:49:42.57 Abs.hold @08:49:42.87 SamplePump.in :start @08:49:42.96 SamplePump.seek 10ml,1:10 @08:49:43.56 SamplePump.configure :prime @08:49:55.22 Abs.stop @08:49:55.30 SamplePump.setPosition! 0ml @08:49:55.39 Exhaust.open @08:49:56.95 Bypass.close @08:49:58.66 Abs.in :default @08:49:58.76 Abs.hold @08:49:59.10 SamplePump.in :start @08:49:59.20 SamplePump.seek 10ml,1:10 @08:49:59.79 SamplePump.configure :prime @08:50:11.46 Abs.stop @08:50:11.54 Toroid.seek :cartridge @08:50:15.90 Sampling 25.0ml @08:50:15.92 SamplePump.setPosition! 0ml @08:50:16.19 Abs.in :default @08:50:16.28 Abs.hold @08:50:16.63 SamplePump.in :start @08:50:16.77 SamplePump.seek 25ml,2:10 @08:50:17.32 SamplePump.configure :sample @08:50:46.80 Abs.stop @08:50:46.88 Intake.close @08:50:46.96 Exhaust.close @08:50:47.07 Handoff.select :close @08:50:47.14 Toroid.seek :bypassed @08:50:51.60 Sampled 25.0ml @08:50:51.62 delay 10:00 @09:00:51.69 Waiting for nadia to answer "Move INTAKE line to MillQ water" @09:00:51.74 Answered Move INTAKE line to MillQ water"" @09:00:51.76 Toroid.seek :bypassed @09:00:51.90 Priming sample loop w/50ml, bypass w/25ml @09:00:51.92 SamplePump.setPosition! 0ml @09:00:52.02 Bypass.open @09:00:53.79 Intake.open @09:00:55.55 Abs.in :default @09:00:55.63 Abs.hold @09:00:55.89 SamplePump.in :start @09:00:55.99 SamplePump.seek 25ml,2:10 @09:00:56.58 SamplePump.configure :prime @09:01:25.98 Abs.stop @09:01:26.06 SamplePump.setPosition! 0ml @09:01:26.14 Exhaust.open @09:01:27.72 Bypass.close @09:01:29.41 Abs.in :default @09:01:29.49 Abs.hold @09:01:29.82 SamplePump.in :start @09:01:29.91 SamplePump.seek 50ml,3:50 @09:01:30.51 SamplePump.configure :prime @09:02:29.46 Abs.stop @09:02:29.55 Toroid.seek :cartridge @09:02:33.90 Sampling 25.0ml @09:02:33.92 SamplePump.setPosition! 0ml @09:02:34.20 Abs.in :default @09:02:34.28 Abs.hold @09:02:34.62 SamplePump.in :start @09:02:34.74 SamplePump.seek 25ml,2:35 @09:02:35.31 SamplePump.configure :sample @09:03:04.78 Abs.stop @09:03:04.87 Intake.close @09:03:04.95 Exhaust.close @09:03:05.10 Toroid.seek :bypassed @09:03:09.46 Sampled 25.0ml @09:03:10.47 Air.in :unlocking @09:03:10.64 Air.seek :clear @09:03:11.69 Lysis1.in :unlocking @09:03:11.85 Lysis1.seek :clear @09:03:11.94 Plunge.in :unlocking @09:03:12.06 Plunge.seek :clear @09:03:13.21 Portable sample Path clean, ready for next sample @13:58:52.49 -> Cart.fire @13:58:52.63 Lysis1.in :fast @13:58:52.78 Lysis1.seek :locked @13:58:55.38 Plunge.in :default @13:58:55.50 Plunge.seek :locked @13:58:55.74 Air.in :fast @13:58:55.83 Air.seek :locked @13:58:56.86 Powering cartridge bus @13:58:59.58 PuckHeater.configure :default #ambient 22.4C @13:58:59.70 Gate.power :heat,:ON,limit to 5000mA @13:59:00.42 Mounted lysis Cartridge board#J59991 @13:59:00.50 Using default cartridge heat loss curve for HeatingFunctionInStage @13:59:00.85 Air.in :default @13:59:00.94 Toroid.seek :bypassed @13:59:01.01 Air.seek :sealed @13:59:01.24 Priming sample loop w/25ml, bypass w/10ml @13:59:01.27 SamplePump.setPosition! 0ml @13:59:01.40 Bypass.open @13:59:03.09 Intake.open @13:59:04.14 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:59:06.80 Vacuum tare is 52% of full scale at 22C (drifted 1%) @13:59:07.83 Abs.hold @13:59:08.23 SamplePump.in :start @13:59:08.33 SamplePump.seek 10ml,1:10 @13:59:08.91 SamplePump.configure :prime @13:59:20.65 Abs.stop @13:59:20.79 SamplePump.setPosition! 0ml @13:59:20.88 Exhaust.open @13:59:22.46 Bypass.close @13:59:24.23 Abs.in :default @13:59:24.33 Abs.hold @13:59:24.82 SamplePump.in :start @13:59:24.92 SamplePump.seek 25ml,2:10 @13:59:25.51 SamplePump.configure :prime @13:59:54.98 Abs.stop @13:59:55.09 Bypass.open @13:59:56.00 Intake.close @13:59:56.91 Toroid.seek :crack @14:00:01.23 Toroid.seek :cartridge @14:00:02.40 Pressurizing cartridge to 26psi above 13.6psia @14:00:02.43 SamplePump.setPosition! 0ml @14:00:02.55 Intake.open @14:00:03.45 Bypass.close @14:00:04.32 Exhaust.close @14:00:05.37 Abs.in :default @14:00:05.49 Abs.hold @14:00:05.81 SamplePump.in :charge @14:00:05.92 SamplePump.seek 2.3ml,40 seconds @14:00:22.21 Abs.stop @14:00:22.35 Intake.close @14:00:23.37 Pressurized cartridge with 1.217ml to 26.6psi @14:00:23.39 Isolated @40.3psia (26.7psi) @14:00:23.45 Verifying cartridge holds at least 0.65..0.85 of 40.3psia @14:00:23.54 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:00:28.19 Cartridge pressure fell 3% to 39.0psia @14:00:28.23 Toroid.seek :cartridge @14:00:28.40 Sampling 1000.0ml @14:00:28.42 SamplePump.setPosition! 0ml @14:00:28.54 Exhaust.open @14:00:29.43 Intake.open @14:00:30.55 Abs.in :default @14:00:30.64 Abs.hold @14:00:31.12 SamplePump.in :start @14:00:31.29 SamplePump.seek 1000ml,1:07:10 @14:00:31.81 SamplePump.configure :sample @14:00:34.23 filtering 2/1000ml at 0.83ml/s with 21.8psi vacuum @14:01:34.31 filtering 51/1000ml at 0.82ml/s with 22.7psi vacuum @14:02:34.30 filtering 99/1000ml at 0.79ml/s with 23.0psi vacuum @14:03:34.29 filtering 145/1000ml at 0.76ml/s with 18.1psi vacuum @14:04:34.30 filtering 189/1000ml at 0.74ml/s with 26.2psi vacuum @14:05:34.30 filtering 232/1000ml at 0.72ml/s with 24.4psi vacuum @14:06:34.31 filtering 274/1000ml at 0.70ml/s with 22.6psi vacuum @14:07:34.31 filtering 315/1000ml at 0.68ml/s with 19.2psi vacuum @14:08:34.30 filtering 355/1000ml at 0.66ml/s with 26.2psi vacuum @14:09:34.29 filtering 393/1000ml at 0.65ml/s with 17.4psi vacuum @14:10:34.29 filtering 431/1000ml at 0.63ml/s with 24.8psi vacuum @14:11:34.30 filtering 468/1000ml at 0.61ml/s with 24.8psi vacuum @14:12:34.36 filtering 504/1000ml at 0.60ml/s with 19.1psi vacuum @14:13:34.31 filtering 539/1000ml at 0.59ml/s with 23.1psi vacuum @14:14:34.33 filtering 573/1000ml at 0.57ml/s with 25.3psi vacuum @14:15:34.31 filtering 607/1000ml at 0.56ml/s with 28.6psi vacuum @14:16:34.29 filtering 640/1000ml at 0.55ml/s with 25.0psi vacuum @14:17:34.30 filtering 672/1000ml at 0.54ml/s with 20.1psi vacuum @14:18:34.30 filtering 704/1000ml at 0.53ml/s with 19.2psi vacuum @14:19:34.29 filtering 735/1000ml at 0.52ml/s with 22.3psi vacuum @14:20:34.30 filtering 766/1000ml at 0.51ml/s with 24.6psi vacuum @14:21:34.31 filtering 796/1000ml at 0.50ml/s with 24.1psi vacuum @14:22:34.30 filtering 825/1000ml at 0.49ml/s with 25.1psi vacuum @14:23:34.29 filtering 854/1000ml at 0.48ml/s with 22.4psi vacuum @14:24:34.29 filtering 883/1000ml at 0.48ml/s with 24.5psi vacuum @14:25:35.15 filtering 911/1000ml at 0.48ml/s with 23.3psi vacuum @14:26:34.31 filtering 939/1000ml at 0.46ml/s with 27.3psi vacuum @14:27:34.30 filtering 966/1000ml at 0.46ml/s with 24.1psi vacuum @14:28:34.31 filtering 993/1000ml at 0.45ml/s with 19.4psi vacuum @14:28:50.47 Abs.stop @14:28:50.60 Intake.close @14:28:50.77 Exhaust.close @14:28:50.88 Toroid.seek :bypassed @14:28:55.33 Sampled 1000.0ml @14:28:55.39 Toroid.seek :bypassed @14:28:55.56 delay 5 seconds @14:29:00.59 Handoff.select :waste @14:29:00.83 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:29:07.48 Syringe tare is 51% of full scale at 22C (drifted 1%) @14:29:07.53 PinPort.in :locking @14:29:07.66 PinPort.seek :locked @14:29:08.12 Archive.in :fast @14:29:08.19 Lysis2.in :fast @14:29:08.29 Lysis1.seek 1250uL @14:29:08.50 Archive.seek :empty @14:29:08.64 Lysis2.seek :empty @14:29:17.11 Evacuating puck with filter bubble point of 50psi @14:29:17.18 PinPort.seek :locked @14:29:17.31 Handoff.select :waste @14:29:17.38 Plunge.seek :puckBottom @14:29:18.78 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:29:26.22 Air syringe pumping up to 1 strokes to reach 8psi @14:29:26.23 Air stroke #1 of 1 @14:29:26.42 Air.in :pressurize @14:29:26.54 Air.seek :pressurized @14:29:32.00 0.1psi after pressurize @14:29:32.03 Air.in :compress @14:29:32.16 Air.seek :evac @14:29:39.80 2.3psi after compress @14:29:50.91 0.3psi after compress (stable) @14:29:50.93 Air.in :default @14:29:51.09 Air.hold @14:29:51.38 at 0.2psi Opened to waste @14:29:51.39 Handoff.select :blocked @14:29:51.61 Plunge.seek :puckTop @14:29:52.54 Air syringe pumping up to 8 strokes to reach 50psi @14:29:52.55 Air stroke #1 of 8 @14:29:52.75 Air.in :compress @14:29:52.87 Air.seek :evac @14:29:53.11 0.2psi after compress @14:30:03.74 0.3psi after compress (stable) @14:30:03.78 Air.in :gulp @14:30:03.92 Air.seek :topVent @14:30:10.58 0.3psi after gulp @14:30:16.18 0.3psi after gulp (stable) @14:30:16.19 Air stroke #2 of 8 @14:30:16.37 Air.in :pressurize @14:30:16.48 Air.seek :pressurized @14:30:22.89 0.4psi after pressurize @14:30:22.92 Air.in :compress @14:30:23.05 Air.seek :evac @14:30:30.74 15.2psi after compress @14:30:41.36 17.0psi after compress (stable) @14:30:42.15 Air.in :gulp @14:30:42.33 Air.seek :topVent @14:30:49.01 17.1psi after gulp @14:30:54.62 17.0psi after gulp (stable) @14:30:54.63 Air stroke #3 of 8 @14:30:54.87 Air.in :pressurize @14:30:54.96 Air.seek :pressurized @14:31:01.37 17.1psi after pressurize @14:31:01.41 Air.in :compress @14:31:01.54 Air.seek :evac @14:31:09.17 31.0psi after compress @14:31:19.81 32.9psi after compress (stable) @14:31:19.86 Air.in :gulp @14:31:20.01 Air.seek :topVent @14:31:26.73 32.9psi after gulp @14:31:32.33 32.8psi after gulp (stable) @14:31:32.45 Air stroke #4 of 8 @14:31:32.63 Air.in :pressurize @14:31:32.78 Air.seek :pressurized @14:31:39.19 32.8psi after pressurize @14:31:39.22 Air.in :compress @14:31:39.34 Air.seek :evac @14:31:46.99 45.7psi after compress @14:31:57.58 47.8psi after compress (stable) @14:31:57.64 Air.in :gulp @14:31:57.79 Air.seek :topVent @14:32:04.47 47.8psi after gulp @14:32:10.58 47.8psi after gulp (stable) @14:32:10.60 Air stroke #5 of 8 @14:32:11.49 Air.in :pressurize @14:32:11.71 Air.seek :pressurized @14:32:18.09 47.8psi after pressurize @14:32:18.14 Air.in :compress @14:32:18.27 Air.seek :evac @14:32:24.05 Reached 50.8 of 50.0psi after pumping 8946uL (4.94 strokes) @14:32:24.07 Air.in :default @14:32:24.19 Air.hold @14:32:24.34 Plunge.seek :puckBottom @14:32:25.27 delay 3 seconds @14:32:28.29 Handoff.select :waste @14:32:28.49 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:32:45.10 delay 1:00 @14:33:45.13 Handoff.select :blocked @14:33:45.33 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:34:07.92 Handoff.select :waste @14:34:08.12 2.4psi at evacEnd @14:34:08.14 Handoff.select :blocked @14:34:08.33 Plunge.seek :puckTop @14:34:09.26 Air.in :fast @14:34:09.35 Air.seek :topVent @14:34:15.68 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @14:34:22.30 Stable @23.6psi after Handoff blocked @14:34:22.39 Air syringe pumping up to 8 strokes to reach 35psi @14:34:22.43 Air stroke #1 of 8 @14:34:22.63 Air.in :pressurize @14:34:22.77 Air.seek :pressurized @14:34:29.18 23.4psi after pressurize @14:34:29.21 Air.in :compress @14:34:29.34 Air.seek :evac @14:34:36.88 Reached 35.5 of 35.0psi after pumping 1801uL (0.99 strokes) @14:34:36.90 Air.in :default @14:34:37.03 Air.hold @14:34:37.18 Plunge.seek :puckBottom @14:34:38.12 delay 3 seconds @14:34:41.14 Handoff.select :waste @14:34:41.31 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:34:49.44 delay 1:00 @14:35:49.47 Handoff.select :blocked @14:35:49.67 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:36:12.30 Handoff.select :waste @14:36:12.48 0.0psi at evacEnd @14:36:12.51 Plunge.seek :pTop @14:36:13.47 delay 5 seconds @14:36:18.50 Air.in :glide @14:36:18.60 Air.seek :fullVent @14:36:20.08 delay 2 seconds @14:36:22.12 Plunge.seek :pBottom @14:36:23.03 Seawater evacuated, Pressure above/below filter released. @14:36:23.06 Plunge.seek :locked @14:36:24.48 Handoff.select :waste @14:36:24.62 Lysis1.in :slow @14:36:24.76 Lysis1.seek 950uL @14:36:38.89 delay 5 seconds @14:36:43.92 Handoff.select :blocked @14:36:44.12 Plunge.seek :puckTop @14:36:45.19 Lysis1.seek 350uL @14:37:13.24 delay 3 seconds @14:37:16.35 Air.in mix with 5.31227 parts Lysis1 @14:37:17.47 Air.seek :evac @14:37:17.55 Lysis1.seek 190uL @14:37:25.31 Air.in :fast @14:37:25.41 Air.seek :full @14:37:32.11 Lysis1.seek :empty @14:37:41.32 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @11.7psi @14:37:41.36 Plunge.seek :puckBottom @14:37:42.46 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @10.8psi @14:37:42.50 Polling Can every 2:00 @14:37:43.07 PuckHeater.configure :default #ambient 22.0C @14:37:43.18 PuckHeater.seek 70.4C @14:39:00.42 PuckHeater.hold 2:00,70.4C @14:41:00.40 Polling Can every 2:00 @14:41:00.97 PuckHeater.configure :default #ambient 22.0C @14:41:01.07 PuckHeater.seek 69.4C @14:41:01.34 PuckHeater.hold 2:00,69.4C @14:43:01.38 Polling Can every 2:00 @14:43:01.94 PuckHeater.configure :default #ambient 22.0C @14:43:02.05 PuckHeater.hold 18:00,68.3C @15:01:00.29 Polling Can every 2:00 @15:01:00.86 PuckHeater.configure :default #ambient 21.9C @15:01:00.96 PuckHeater.seek 104.3C @15:02:04.87 PuckHeater.hold 2:00,104.3C @15:04:04.78 Polling Can every 2:00 @15:04:05.33 PuckHeater.configure :default #ambient 21.8C @15:04:05.45 PuckHeater.seek 103.3C @15:04:05.75 PuckHeater.hold 2:00,103.3C @15:06:05.70 Polling Can every 2:00 @15:06:06.26 PuckHeater.configure :default #ambient 21.9C @15:06:06.36 PuckHeater.hold 2:00,102.2C @15:08:06.33 delay 5:00 @15:13:06.42 Lysate cooked @6.4psi @15:13:06.46 Plunge.seek :puckTop @15:13:08.26 Air syringe pumping up to 5 strokes to reach 40psi @15:13:08.27 Air stroke #1 of 5 @15:13:08.45 Air.in :pressurize @15:13:08.55 Air.seek :pressurized @15:13:14.94 6.8psi after pressurize @15:13:14.99 Air.in :compress @15:13:15.13 Air.seek :evac @15:13:20.75 Reached 41.5 of 40.0psi after pumping 1693uL (0.93 strokes) @15:13:20.78 Air.in :default @15:13:20.91 Air.hold @15:13:21.04 delay 2 seconds @15:13:23.08 Air.in :fast @15:13:23.21 Air.seek :full @15:13:29.50 Plunge.seek :puckBottom @15:13:30.53 Lysis1.seek 138uL @15:13:37.12 delay 2 seconds @15:13:39.16 Plunge.seek :puckTop @15:13:40.08 Lysis1.seek :empty @15:13:46.75 Plunge.seek :puckBottom @15:13:47.87 Lysis1.in :extract @15:13:48.01 Lysis1.seek 675uL,nil @15:13:50.53 Air.in :pressurize @15:13:50.63 Air.seek :pressurized @15:13:51.20 Syringe::LysatePlungerStuck in nadia -- at < 304uL during Lysis Syringe #1 (LyS1) move @15:13:51.26 Lysis1.in :default @15:13:51.38 Lysis1.seek 274uL @15:13:57.12 14.5psi after pressurize @15:13:57.15 Air.in :compressLysate @15:13:57.28 Air.seek :evac @15:14:03.09 Reached 15.8 of 15.5psi after pumping 1468uL (0.81 strokes) @15:14:03.11 Air.in :default @15:14:03.23 Air.hold @15:14:03.48 Lysis1.in :extract @15:14:03.66 Lysis1.seek 675uL,nil @15:14:05.10 Air.in :compressLysate @15:14:05.25 Air.seek :evac @15:14:20.38 Air.in :default @15:14:20.56 Air.hold @15:14:20.92 Pulled 675uL lysate @14.8psi @15:14:21.20 Lysis1.seek 800uL,nil @15:14:22.60 Air.in :compressLysate @15:14:22.75 Air.seek :evac @15:14:31.86 13.6psi after compress @15:14:42.68 14.2psi after compress (stable) @15:14:48.21 Air.in :default @15:14:48.30 Air.hold @15:14:48.44 Lysis1.in :default @15:14:48.56 Lysis1.hold @15:14:48.91 Air.in :glide @15:14:49.00 Air.seek :fullVent @15:14:50.23 Handoff.select :waste @15:14:50.40 Recovered 703uL lysate @14.1psi @15:14:50.44 delay 3 seconds @15:14:53.54 After 3 delay Recovered 703uL lysate @-0.7psi @15:14:53.58 delay 5 seconds @15:14:58.64 Gate.power :heat,:OFF @15:14:58.67 Gate.power :cartridge,:OFF @15:14:58.86 PinPort.in :unlocking @15:14:58.95 PinPort.seek :clear @15:14:59.48 Archive.in :unlocking @15:14:59.68 Archive.seek :clear @15:14:59.86 Air.in :unlocking @15:15:00.01 Air.seek :clear @15:15:00.16 Lysis2.in :unlocking @15:15:00.26 Lysis2.seek :clear @15:15:05.06 Lysis1.in :unlocking @15:15:05.28 Lysis1.seek :clear @15:15:05.41 Plunge.in :unlocking @15:15:05.50 Plunge.seek :clear @15:18:57.34 -> cleanPortable @15:18:58.77 Waiting for nadia to answer "LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue" @15:19:00.48 Answered LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue"" @15:19:00.61 Lysis1.in :fast @15:19:00.72 Lysis1.seek :locked @15:19:03.38 Plunge.in :default @15:19:03.49 Plunge.seek :locked @15:19:03.74 Air.in :fast @15:19:03.84 Air.seek :locked @15:19:04.85 Powering cartridge bus @15:19:07.64 Gate.power :cartridge,:OFF @15:19:08.22 Mounted archive Cartridge @15:19:08.31 Toroid.seek :bypassed @15:19:08.43 Priming sample loop w/10ml, bypass w/10ml @15:19:08.46 SamplePump.setPosition! 0ml @15:19:08.57 Bypass.open @15:19:10.24 Intake.open @15:19:11.26 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:19:13.89 Vacuum tare is 53% of full scale at 22C (drifted 1%) @15:19:14.12 Abs.in :default @15:19:14.24 Abs.hold @15:19:14.57 SamplePump.in :start @15:19:14.67 SamplePump.seek 10ml,1:10 @15:19:15.25 SamplePump.configure :prime @15:19:27.02 Abs.stop @15:19:27.13 SamplePump.setPosition! 0ml @15:19:27.25 Exhaust.open @15:19:28.90 Bypass.close @15:19:30.65 Abs.in :default @15:19:30.78 Abs.hold @15:19:31.21 SamplePump.in :start @15:19:31.32 SamplePump.seek 10ml,1:10 @15:19:31.90 SamplePump.configure :prime @15:19:43.62 Abs.stop @15:19:43.72 Waiting for nadia to answer "INTAKE line plumbed to 10% Bleach, push return to continue" @15:24:14.97 Answered INTAKE line plumbed to 10% Bleach, push return to continue"" @15:24:15.01 Toroid.seek :bypassed @15:24:15.16 Priming sample loop w/10ml, bypass w/10ml @15:24:15.19 SamplePump.setPosition! 0ml @15:24:15.29 Exhaust.close @15:24:16.14 Bypass.open @15:24:18.90 Abs.in :default @15:24:19.00 Abs.hold @15:24:19.34 SamplePump.in :start @15:24:19.45 SamplePump.seek 10ml,1:10 @15:24:20.03 SamplePump.configure :prime @15:24:31.77 Abs.stop @15:24:31.87 SamplePump.setPosition! 0ml @15:24:31.98 Exhaust.open @15:24:33.56 Bypass.close @15:24:35.33 Abs.in :default @15:24:35.41 Abs.hold @15:24:35.81 SamplePump.in :start @15:24:35.92 SamplePump.seek 10ml,1:10 @15:24:36.50 SamplePump.configure :prime @15:24:48.23 Abs.stop @15:24:48.34 Toroid.seek :cartridge @15:24:52.77 Sampling 25.0ml @15:24:52.79 SamplePump.setPosition! 0ml @15:24:53.13 Abs.in :default @15:24:53.23 Abs.hold @15:24:53.61 SamplePump.in :start @15:24:53.72 SamplePump.seek 25ml,2:10 @15:24:54.30 SamplePump.configure :sample @15:25:23.79 Abs.stop @15:25:23.89 Intake.close @15:25:24.01 Exhaust.close @15:25:24.15 Handoff.select :close @15:25:24.23 Toroid.seek :bypassed @15:25:28.77 Sampled 25.0ml @15:25:28.80 delay 10:00 @15:35:28.85 Waiting for nadia to answer "Move INTAKE line to MillQ water" @15:36:12.85 Answered Move INTAKE line to MillQ water"" @15:36:12.88 Toroid.seek :bypassed @15:36:13.05 Priming sample loop w/50ml, bypass w/25ml @15:36:13.07 SamplePump.setPosition! 0ml @15:36:13.19 Bypass.open @15:36:14.98 Intake.open @15:36:16.80 Abs.in :default @15:36:16.91 Abs.hold @15:36:17.21 SamplePump.in :start @15:36:17.33 SamplePump.seek 25ml,2:10 @15:36:18.26 SamplePump.configure :prime @15:36:48.08 Abs.stop @15:36:48.19 SamplePump.setPosition! 0ml @15:36:48.29 Exhaust.open @15:36:49.83 Bypass.close @15:36:51.58 Abs.in :default @15:36:51.67 Abs.hold @15:36:52.07 SamplePump.in :start @15:36:52.19 SamplePump.seek 50ml,3:50 @15:36:52.77 SamplePump.configure :prime @15:37:51.84 Abs.stop @15:37:51.94 Toroid.seek :cartridge @15:37:56.33 Sampling 25.0ml @15:37:56.34 SamplePump.setPosition! 0ml @15:37:56.70 Abs.in :default @15:37:56.85 Abs.hold @15:37:57.27 SamplePump.in :start @15:37:57.38 SamplePump.seek 25ml,2:35 @15:37:57.95 SamplePump.configure :sample @15:38:27.43 Abs.stop @15:38:27.54 Intake.close @15:38:27.66 Exhaust.close @15:38:27.76 Toroid.seek :bypassed @15:38:32.21 Sampled 25.0ml @15:38:32.56 Air.in :unlocking @15:38:32.81 Air.seek :clear @15:38:33.86 Lysis1.in :unlocking @15:38:34.02 Lysis1.seek :clear @15:38:34.19 Plunge.in :unlocking @15:38:34.31 Plunge.seek :clear @15:38:35.36 Portable sample Path clean, ready for next sample @15:44:51.38 -> ESP.ready! @15:44:52.23 Toroid.seek :clear @15:44:54.51 Air.in :default @15:44:54.62 Air.seek :clear @15:44:54.79 Lysis1.in :default @15:44:54.88 Lysis1.seek :clear @15:44:55.02 Lysis2.in :default @15:44:55.13 Lysis2.seek :clear @15:44:56.04 Archive.in :default @15:44:56.15 Archive.seek :clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 25ml Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Abs = Absolute Pressure (Leak) Sensor @14psia Toroid = Toroid Valve (TV) clear PinPort = Handoff Pin Port (PP) clear Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear @15:45:12.81 -> ESP.reready! @15:45:12.86 Plunge.in :default @15:45:12.95 Toroid.in :default @15:45:13.06 PinPort.in :default @15:45:13.32 Archive.in :default @15:45:13.43 Lysis1.in :default @15:45:13.52 Air.in :default @15:45:13.56 Lysis2.in :default @15:45:13.63 Toroid.home.jog 0 @15:45:13.69 Plunge.home.jog 0 @15:45:13.88 PinPort.home.jog 0 @15:45:14.16 Archive.home.jog 0 @15:45:14.25 Lysis1.home.jog 0 @15:45:14.39 Air.home.jog 0 @15:45:14.45 Lysis2.home.jog 0 @15:45:15.53 Toroid.in :homing @15:45:15.64 Plunge.in :homing @15:45:15.85 Toroid.jog 824 @15:45:15.88 Plunge.jog 550 @15:45:16.21 Lysis1.in :homing @15:45:16.28 Archive.in :homing @15:45:16.32 Lysis2.in :homing @15:45:16.39 Air.in :homing @15:45:16.45 PinPort.in :homing @15:45:16.77 Lysis1.jog 1665 @15:45:16.86 Lysis2.jog 1612 @15:45:16.92 Archive.jog 1620 @15:45:16.98 Air.jog 250 @15:45:17.09 PinPort.jog -707 @15:45:17.50 Plunge.setRawPosition! 50 @15:45:17.58 Plunge.seek :home @15:45:17.87 Air.setRawPosition! 50 @15:45:18.00 Plunge.in :default @15:45:18.03 Air.seek :home @15:45:18.39 Lysis1.setRawPosition! 50 @15:45:18.42 PinPort.setRawPosition! -50 @15:45:18.60 Lysis1.seek :home @15:45:18.77 PinPort.seek :home @15:45:19.04 Lysis2.setRawPosition! 50 @15:45:19.09 Air.in :default @15:45:19.12 Archive.setRawPosition! 50 @15:45:19.17 Toroid.setRawPosition! 0 @15:45:19.40 Lysis2.seek :home @15:45:19.56 Archive.seek :home @15:45:19.62 Toroid.seek :home @15:45:19.97 Lysis2.in :default @15:45:20.02 Lysis1.in :default @15:45:20.06 PinPort.in :default @15:45:20.12 Toroid.in :default @15:45:20.44 Archive.in :default @15:45:21.47 Toroid.seek :clear @15:45:21.71 PinPort.in :unlocking @15:45:21.82 PinPort.seek :clear @15:45:23.81 Air.seek :clear @15:45:24.51 Lysis1.seek :clear @15:45:25.45 Lysis2.seek :clear @15:45:26.27 Archive.seek :clear Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 25ml Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Abs = Absolute Pressure (Leak) Sensor @14psia Toroid = Toroid Valve (TV) clear PinPort = Handoff Pin Port (PP) clear Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear @15:45:36.29 -> Cart.fire @15:45:36.45 Lysis1.in :fast @15:45:36.56 Lysis1.seek :locked @15:45:39.15 Plunge.seek :locked @15:45:39.39 Air.in :fast @15:45:39.48 Air.seek :locked @15:45:40.49 Powering cartridge bus @15:45:43.28 PuckHeater.configure :default #ambient 21.9C @15:45:43.39 Gate.power :heat,:ON,limit to 5000mA @15:45:44.15 Mounted lysis Cartridge board#J59946 @15:45:44.23 Using this cartridge's heat loss curve for HeatingFunctionInStage @15:45:44.55 Air.in :default @15:45:44.59 Toroid.seek :bypassed @15:45:44.82 Air.seek :sealed @15:45:46.97 Priming sample loop w/25ml, bypass w/10ml @15:45:47.00 SamplePump.setPosition! 0ml @15:45:47.11 Bypass.open @15:45:48.87 Intake.open @15:45:49.90 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:45:52.53 Vacuum tare is 53% of full scale at 22C (drifted 1%) @15:45:52.82 Abs.in :default @15:45:52.91 Abs.hold @15:45:53.32 SamplePump.in :start @15:45:53.43 SamplePump.seek 10ml,1:10 @15:45:53.94 SamplePump.configure :prime @15:46:05.74 Abs.stop @15:46:05.83 SamplePump.setPosition! 0ml @15:46:05.94 Exhaust.open @15:46:07.51 Bypass.close @15:46:09.23 Abs.in :default @15:46:09.33 Abs.hold @15:46:09.73 SamplePump.in :start @15:46:09.85 SamplePump.seek 25ml,2:10 @15:46:10.42 SamplePump.configure :prime @15:46:39.92 Abs.stop @15:46:40.02 Bypass.open @15:46:40.92 Intake.close @15:46:41.82 Toroid.seek :crack @15:46:46.14 Toroid.seek :cartridge @15:46:47.31 Pressurizing cartridge to 26psi above 13.5psia @15:46:47.34 SamplePump.setPosition! 0ml @15:46:47.44 Intake.open @15:46:48.36 Bypass.close @15:46:49.19 Exhaust.close @15:46:50.19 Abs.in :default @15:46:50.30 Abs.hold @15:46:50.61 SamplePump.in :charge @15:46:50.76 SamplePump.seek 2.3ml,40 seconds @15:47:06.87 Abs.stop @15:47:06.98 Intake.close @15:47:07.92 Pressurized cartridge with 1.182ml to 27.1psi @15:47:07.94 Isolated @39.0psia (25.5psi) @15:47:08.01 Verifying cartridge holds at least 0.65..0.85 of 39.0psia @15:47:08.06 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:47:12.72 Cartridge pressure fell 1% to 38.4psia @15:47:12.78 Toroid.seek :cartridge @15:47:12.98 Sampling 1000.0ml @15:47:13.00 SamplePump.setPosition! 0ml @15:47:13.12 Exhaust.open @15:47:13.94 Intake.open @15:47:16.01 Abs.in :default @15:47:16.10 Abs.hold @15:47:16.54 SamplePump.in :start @15:47:16.65 SamplePump.seek 1000ml,1:07:10 @15:47:17.23 SamplePump.configure :sample @15:47:19.67 filtering 2/1000ml at 0.84ml/s with 21.2psi vacuum @15:48:19.72 filtering 52/1000ml at 0.83ml/s with 15.4psi vacuum @15:49:19.72 filtering 101/1000ml at 0.82ml/s with 19.5psi vacuum @15:50:19.75 filtering 150/1000ml at 0.81ml/s with 16.6psi vacuum @15:51:19.72 filtering 198/1000ml at 0.80ml/s with 23.0psi vacuum @15:52:19.72 filtering 246/1000ml at 0.79ml/s with 20.0psi vacuum @15:53:19.79 filtering 293/1000ml at 0.78ml/s with 20.2psi vacuum @15:54:19.73 filtering 339/1000ml at 0.77ml/s with 20.2psi vacuum @15:55:19.73 filtering 385/1000ml at 0.76ml/s with 23.9psi vacuum @15:56:19.73 filtering 430/1000ml at 0.75ml/s with 24.5psi vacuum @15:57:19.72 filtering 474/1000ml at 0.74ml/s with 24.2psi vacuum @15:58:19.72 filtering 517/1000ml at 0.72ml/s with 24.9psi vacuum @15:59:19.73 filtering 560/1000ml at 0.71ml/s with 18.3psi vacuum @16:00:19.74 filtering 602/1000ml at 0.70ml/s with 26.9psi vacuum @16:01:19.75 filtering 644/1000ml at 0.69ml/s with 15.8psi vacuum @16:02:19.73 filtering 685/1000ml at 0.68ml/s with 24.4psi vacuum @16:03:19.74 filtering 725/1000ml at 0.67ml/s with 22.0psi vacuum @16:04:19.72 filtering 764/1000ml at 0.66ml/s with 25.2psi vacuum @16:05:19.72 filtering 803/1000ml at 0.65ml/s with 20.7psi vacuum @16:06:19.72 filtering 842/1000ml at 0.64ml/s with 25.5psi vacuum @16:07:19.72 filtering 880/1000ml at 0.64ml/s with 21.9psi vacuum @16:08:19.78 filtering 918/1000ml at 0.63ml/s with 23.3psi vacuum @16:09:19.74 filtering 955/1000ml at 0.62ml/s with 25.5psi vacuum @16:10:19.72 filtering 992/1000ml at 0.62ml/s with 22.8psi vacuum @16:10:32.50 Abs.stop @16:10:32.65 Intake.close @16:10:32.81 Exhaust.close @16:10:32.89 Toroid.seek :bypassed @16:10:37.35 Sampled 1000.0ml @16:10:37.42 Toroid.seek :bypassed @16:10:37.62 delay 5 seconds @16:10:43.46 Handoff.select :waste @16:10:43.66 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:10:50.31 Syringe tare is 51% of full scale at 22C (drifted 1%) @16:10:50.37 PinPort.in :locking @16:10:50.51 PinPort.seek :locked @16:10:50.97 Archive.in :fast @16:10:51.05 Lysis2.in :fast @16:10:51.17 Lysis1.seek 1250uL @16:10:51.35 Archive.seek :empty @16:10:51.47 Lysis2.seek :empty @16:10:59.89 Evacuating puck with filter bubble point of 50psi @16:10:59.96 PinPort.seek :locked @16:11:00.09 Handoff.select :waste @16:11:00.14 Plunge.seek :puckBottom @16:11:01.56 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:11:08.17 Air syringe pumping up to 1 strokes to reach 8psi @16:11:08.19 Air stroke #1 of 1 @16:11:08.38 Air.in :pressurize @16:11:08.49 Air.seek :pressurized @16:11:13.89 0.2psi after pressurize @16:11:13.92 Air.in :compress @16:11:14.03 Air.seek :evac @16:11:21.67 0.0psi after compress @16:11:32.29 -0.0psi after compress (stable) @16:11:32.31 Air.in :default @16:11:32.42 Air.hold @16:11:32.75 at 0.1psi Opened to waste @16:11:32.80 Handoff.select :blocked @16:11:33.01 Plunge.seek :puckTop @16:11:33.93 Air syringe pumping up to 8 strokes to reach 50psi @16:11:33.95 Air stroke #1 of 8 @16:11:34.12 Air.in :compress @16:11:34.23 Air.seek :evac @16:11:34.46 15.3psi after compress @16:11:45.07 15.3psi after compress (stable) @16:11:45.11 Air.in :gulp @16:11:45.24 Air.seek :topVent @16:11:51.93 15.1psi after gulp @16:11:57.54 15.1psi after gulp (stable) @16:11:57.57 Air stroke #2 of 8 @16:11:57.74 Air.in :pressurize @16:11:57.84 Air.seek :pressurized @16:12:04.25 15.3psi after pressurize @16:12:04.28 Air.in :compress @16:12:04.44 Air.seek :evac @16:12:12.06 32.7psi after compress @16:12:23.20 35.2psi after compress (stable) @16:12:23.24 Air.in :gulp @16:12:23.36 Air.seek :topVent @16:12:30.03 35.2psi after gulp @16:12:36.35 35.1psi after gulp (stable) @16:12:36.47 Air stroke #3 of 8 @16:12:36.67 Air.in :pressurize @16:12:36.83 Air.seek :pressurized @16:12:43.23 35.1psi after pressurize @16:12:43.27 Air.in :compress @16:12:43.48 Air.seek :evac @16:12:51.12 Reached 50.8 of 50.0psi after pumping 5431uL (3.00 strokes) @16:12:51.16 Air.in :default @16:12:51.30 Air.hold @16:12:51.44 Plunge.seek :puckBottom @16:12:52.38 delay 3 seconds @16:12:55.40 Handoff.select :waste @16:12:55.60 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:13:11.71 delay 1:00 @16:14:11.74 Handoff.select :blocked @16:14:11.94 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @16:14:34.55 Handoff.select :waste @16:14:34.74 0.9psi at evacEnd @16:14:34.79 Handoff.select :blocked @16:14:35.00 Plunge.seek :puckTop @16:14:36.00 Air.in :fast @16:14:36.10 Air.seek :topVent @16:14:42.82 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @16:14:49.42 Stable @33.0psi after Handoff blocked @16:14:49.46 Air syringe pumping up to 8 strokes to reach 35psi @16:14:49.53 Air stroke #1 of 8 @16:14:49.69 Air.in :pressurize @16:14:49.79 Air.seek :pressurized @16:14:56.21 33.1psi after pressurize @16:14:56.26 Air.in :compress @16:14:56.40 Air.seek :evac @16:15:01.73 Reached 35.9 of 35.0psi after pumping 1678uL (0.93 strokes) @16:15:01.76 Air.in :default @16:15:01.88 Air.hold @16:15:02.01 Plunge.seek :puckBottom @16:15:02.93 delay 3 seconds @16:15:05.96 Handoff.select :waste @16:15:06.15 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:15:17.26 delay 1:00 @16:16:17.30 Handoff.select :blocked @16:16:17.55 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @16:16:40.18 Handoff.select :waste @16:16:40.37 0.7psi at evacEnd @16:16:40.43 Plunge.seek :pTop @16:16:41.35 delay 5 seconds @16:16:46.38 Air.in :glide @16:16:46.48 Air.seek :fullVent @16:16:49.26 delay 2 seconds @16:16:51.29 Plunge.seek :pBottom @16:16:52.19 Seawater evacuated, Pressure above/below filter released. @16:16:52.23 Plunge.seek :locked @16:16:53.67 Handoff.select :waste @16:16:53.84 Lysis1.in :slow @16:16:53.91 Lysis1.seek 950uL @16:17:08.03 delay 5 seconds @16:17:13.06 Handoff.select :blocked @16:17:13.30 Plunge.seek :puckTop @16:17:14.39 Lysis1.seek 350uL @16:17:42.42 delay 3 seconds @16:17:45.53 Air.in mix with 5.31227 parts Lysis1 @16:17:47.32 Air.seek :evac @16:17:47.37 Lysis1.seek 190uL @16:17:55.13 Air.in :fast @16:17:55.24 Air.seek :full @16:18:01.96 Lysis1.seek :empty @16:18:11.17 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @17.2psi @16:18:11.19 Plunge.seek :puckBottom @16:18:12.29 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @16.4psi @16:18:12.32 Polling Can every 2:00 @16:18:12.92 PuckHeater.configure :default #ambient 22.1C @16:18:13.01 PuckHeater.seek 70.4C @16:19:27.87 PuckHeater.hold 2:00,70.4C @16:21:27.96 Polling Can every 2:00 @16:21:28.53 PuckHeater.configure :default #ambient 22.2C @16:21:28.63 PuckHeater.seek 69.4C @16:21:28.92 PuckHeater.hold 2:00,69.4C @16:23:29.00 Polling Can every 2:00 @16:23:29.56 PuckHeater.configure :default #ambient 22.1C @16:23:29.68 PuckHeater.hold 18:00,68.3C @16:41:27.83 Polling Can every 2:00 @16:41:28.38 PuckHeater.configure :default #ambient 22.3C @16:41:28.48 PuckHeater.seek 104.3C @16:42:32.40 PuckHeater.hold 2:00,104.3C @16:44:32.40 Polling Can every 2:00 @16:44:32.96 PuckHeater.configure :default #ambient 22.4C @16:44:33.07 PuckHeater.seek 103.3C @16:44:33.31 PuckHeater.hold 2:00,103.3C @16:46:33.17 Polling Can every 2:00 @16:46:33.72 PuckHeater.configure :default #ambient 22.3C @16:46:33.84 PuckHeater.hold 2:00,102.2C @16:48:33.80 delay 5:00 @16:53:33.90 Lysate cooked @10.7psi @16:53:33.93 Plunge.seek :puckTop @16:53:34.87 Air syringe pumping up to 5 strokes to reach 40psi @16:53:34.89 Air stroke #1 of 5 @16:53:35.08 Air.in :pressurize @16:53:36.03 Air.seek :pressurized @16:53:42.45 11.1psi after pressurize @16:53:42.48 Air.in :compress @16:53:42.61 Air.seek :evac @16:53:48.10 Reached 41.8 of 40.0psi after pumping 1686uL (0.93 strokes) @16:53:48.13 Air.in :default @16:53:48.26 Air.hold @16:53:48.39 delay 2 seconds @16:53:50.43 Air.in :fast @16:53:50.53 Air.seek :full @16:53:56.83 Plunge.seek :puckBottom @16:53:57.86 Lysis1.seek 138uL @16:54:04.46 delay 2 seconds @16:54:06.50 Plunge.seek :puckTop @16:54:07.42 Lysis1.seek :empty @16:54:14.06 Plunge.seek :puckBottom @16:54:15.20 Lysis1.in :extract @16:54:15.30 Lysis1.seek 675uL,nil @16:54:17.88 Air.in :pressurize @16:54:18.05 Air.seek :pressurized @16:54:24.44 11.1psi after pressurize @16:54:24.50 Air.in :compressLysate @16:54:24.64 Air.seek :evac @16:54:46.14 Air.in :default @16:54:46.24 Air.hold @16:54:46.44 ThreadError in nadia -- RecoveringLysate thread died holding # @16:54:46.66 Pulled 675uL lysate @15.3psi @16:54:46.98 Lysis1.seek 800uL,nil @16:54:48.37 Air.in :compressLysate @16:54:48.50 Air.seek :evac @16:54:56.96 14.7psi after compress @16:55:08.27 13.0psi after compress (stable) @16:55:13.64 Air.in :default @16:55:13.73 Air.hold @16:55:13.87 Lysis1.in :default @16:55:14.01 Lysis1.hold @16:55:14.30 Air.in :glide @16:55:14.40 Air.seek :fullVent @16:55:15.64 Handoff.select :waste @16:55:15.80 Recovered 778uL lysate @13.2psi @16:55:15.84 delay 3 seconds @16:55:19.00 After 3 delay Recovered 778uL lysate @1.2psi @16:55:19.05 delay 5 seconds @16:55:24.11 Gate.power :heat,:OFF @16:55:24.13 Gate.power :cartridge,:OFF @16:55:24.27 PinPort.in :unlocking @16:55:24.38 PinPort.seek :clear @16:55:24.93 Archive.in :unlocking @16:55:25.08 Archive.seek :clear @16:55:25.23 Air.in :unlocking @16:55:26.27 Air.seek :clear @16:55:26.38 Lysis2.in :unlocking @16:55:26.56 Lysis2.seek :clear @16:55:31.34 Lysis1.in :unlocking @16:55:31.53 Lysis1.seek :clear @16:55:31.68 Plunge.in :unlocking @16:55:31.80 Plunge.seek :clear @16:59:11.27 -> cleanPortable @16:59:11.81 Waiting for nadia to answer "LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue" @16:59:23.04 Answered LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue"" @16:59:23.18 Lysis1.in :fast @16:59:23.29 Lysis1.seek :locked @16:59:25.87 Plunge.in :default @16:59:25.98 Plunge.seek :locked @16:59:26.21 Air.in :fast @16:59:26.31 Air.seek :locked @16:59:27.33 Powering cartridge bus @16:59:30.05 Gate.power :cartridge,:OFF @16:59:30.63 Mounted archive Cartridge @16:59:30.69 Toroid.seek :bypassed @16:59:30.87 Priming sample loop w/10ml, bypass w/10ml @16:59:30.89 SamplePump.setPosition! 0ml @16:59:31.01 Bypass.open @16:59:32.68 Intake.open @16:59:33.70 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:59:36.33 Vacuum tare is 53% of full scale at 22C (drifted 2%) @16:59:36.56 Abs.in :default @16:59:36.66 Abs.hold @16:59:37.02 SamplePump.in :start @16:59:37.13 SamplePump.seek 10ml,1:10 @16:59:37.72 SamplePump.configure :prime @16:59:49.49 Abs.stop @16:59:49.60 SamplePump.setPosition! 0ml @16:59:49.68 Exhaust.open @16:59:51.25 Bypass.close @16:59:53.01 Abs.in :default @16:59:53.09 Abs.hold @16:59:54.43 SamplePump.in :start @16:59:54.55 SamplePump.seek 10ml,1:10 @16:59:55.12 SamplePump.configure :prime @17:00:06.87 Abs.stop @17:00:06.99 Waiting for nadia to answer "INTAKE line plumbed to 10% Bleach, push return to continue" @17:00:13.94 Answered INTAKE line plumbed to 10% Bleach, push return to continue"" @17:00:13.97 Toroid.seek :bypassed @17:00:14.15 Priming sample loop w/10ml, bypass w/10ml @17:00:14.18 SamplePump.setPosition! 0ml @17:00:14.28 Exhaust.close @17:00:15.13 Bypass.open @17:00:17.05 Abs.in :default @17:00:17.15 Abs.hold @17:00:17.47 SamplePump.in :start @17:00:17.58 SamplePump.seek 10ml,1:10 @17:00:18.15 SamplePump.configure :prime @17:00:29.87 Abs.stop @17:00:29.96 SamplePump.setPosition! 0ml @17:00:30.07 Exhaust.open @17:00:31.64 Bypass.close @17:00:33.40 Abs.in :default @17:00:33.49 Abs.hold @17:00:33.90 SamplePump.in :start @17:00:34.02 SamplePump.seek 10ml,1:10 @17:00:34.58 SamplePump.configure :prime @17:00:46.31 Abs.stop @17:00:46.42 Toroid.seek :cartridge @17:00:50.82 Sampling 25.0ml @17:00:50.84 SamplePump.setPosition! 0ml @17:00:51.16 Abs.in :default @17:00:51.26 Abs.hold @17:00:51.66 SamplePump.in :start @17:00:51.78 SamplePump.seek 25ml,2:10 @17:00:52.35 SamplePump.configure :sample @17:01:21.85 Abs.stop @17:01:21.96 Intake.close @17:01:22.02 Exhaust.close @17:01:22.20 Handoff.select :close @17:01:22.33 Toroid.seek :bypassed @17:01:26.80 Sampled 25.0ml @17:01:26.86 delay 10:00 @17:11:26.91 Waiting for nadia to answer "Move INTAKE line to MillQ water" @17:11:26.96 Answered Move INTAKE line to MillQ water"" @17:11:27.00 Toroid.seek :bypassed @17:11:27.97 Priming sample loop w/50ml, bypass w/25ml @17:11:27.99 SamplePump.setPosition! 0ml @17:11:28.10 Bypass.open @17:11:29.91 Intake.open @17:11:31.73 Abs.in :default @17:11:31.83 Abs.hold @17:11:32.18 SamplePump.in :start @17:11:32.30 SamplePump.seek 25ml,2:10 @17:11:32.87 SamplePump.configure :prime @17:12:02.35 Abs.stop @17:12:02.44 SamplePump.setPosition! 0ml @17:12:02.54 Exhaust.open @17:12:04.10 Bypass.close @17:12:05.84 Abs.in :default @17:12:05.91 Abs.hold @17:12:06.34 SamplePump.in :start @17:12:06.45 SamplePump.seek 50ml,3:50 @17:12:07.01 SamplePump.configure :prime @17:13:06.05 Abs.stop @17:13:06.17 Toroid.seek :cartridge @17:13:10.55 Sampling 25.0ml @17:13:10.57 SamplePump.setPosition! 0ml @17:13:10.94 Abs.in :default @17:13:11.05 Abs.hold @17:13:11.47 SamplePump.in :start @17:13:11.59 SamplePump.seek 25ml,2:35 @17:13:12.16 SamplePump.configure :sample @17:13:41.66 Abs.stop @17:13:41.75 Intake.close @17:13:41.89 Exhaust.close @17:13:42.03 Toroid.seek :bypassed @17:13:46.42 Sampled 25.0ml @17:13:46.76 Air.in :unlocking @17:13:46.97 Air.seek :clear @17:13:48.04 Lysis1.in :unlocking @17:13:48.22 Lysis1.seek :clear @17:13:48.39 Plunge.in :unlocking @17:13:48.48 Plunge.seek :clear @17:13:49.53 Portable sample Path clean, ready for next sample @02:32:41.39PDT10-Sep-25 -> Cart.fire @02:32:41.54 Lysis1.in :fast @02:32:41.63 Lysis1.seek :locked @02:32:44.25 Plunge.in :default @02:32:44.36 Plunge.seek :locked @02:32:44.58 Air.in :fast @02:32:44.68 Air.seek :locked @02:32:45.69 Powering cartridge bus @02:32:48.45 PuckHeater.configure :default #ambient 22.3C @02:32:48.57 Gate.power :heat,:ON,limit to 5000mA @02:32:49.27 Mounted lysis Cartridge board#J59966 @02:32:49.34 Using default cartridge heat loss curve for HeatingFunctionInStage @02:32:49.68 Air.in :default @02:32:49.71 Toroid.seek :bypassed @02:32:49.90 Air.seek :sealed @02:32:50.04 Priming sample loop w/25ml, bypass w/10ml @02:32:50.06 SamplePump.setPosition! 0ml @02:32:50.17 Bypass.open @02:32:51.89 Intake.open @02:32:52.95 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @02:32:55.66 Vacuum tare is 53% of full scale at 21C (drifted 1%) @02:32:55.92 Abs.in :default @02:32:56.01 Abs.hold @02:32:56.36 SamplePump.in :start @02:32:56.48 SamplePump.seek 10ml,1:10 @02:32:57.05 SamplePump.configure :prime @02:33:08.79 Abs.stop @02:33:08.94 SamplePump.setPosition! 0ml @02:33:09.04 Exhaust.open @02:33:10.63 Bypass.close @02:33:12.41 Abs.in :default @02:33:12.50 Abs.hold @02:33:12.96 SamplePump.in :start @02:33:13.08 SamplePump.seek 25ml,2:10 @02:33:13.65 SamplePump.configure :prime @02:33:43.15 Abs.stop @02:33:43.26 Bypass.open @02:33:44.16 Intake.close @02:33:45.05 Toroid.seek :crack @02:33:49.37 Toroid.seek :cartridge @02:33:50.56 Pressurizing cartridge to 26psi above 13.8psia @02:33:50.59 SamplePump.setPosition! 0ml @02:33:50.70 Intake.open @02:33:51.60 Bypass.close @02:33:52.49 Exhaust.close @02:33:53.51 Abs.in :default @02:33:53.61 Abs.hold @02:33:53.92 SamplePump.in :charge @02:33:54.04 SamplePump.seek 2.3ml,40 seconds @02:34:09.98 Abs.stop @02:34:10.09 Intake.close @02:34:11.07 Pressurized cartridge with 1.165ml to 26.1psi @02:34:11.08 Isolated @38.4psia (24.7psi) @02:34:11.14 Verifying cartridge holds at least 0.65..0.85 of 38.4psia @02:34:11.22 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @02:34:15.85 Cartridge pressure fell 1% to 38.0psia @02:34:15.91 Toroid.seek :cartridge @02:34:16.07 Sampling 1000.0ml @02:34:16.10 SamplePump.setPosition! 0ml @02:34:16.21 Exhaust.open @02:34:17.08 Intake.open @02:34:18.26 Abs.in :default @02:34:18.37 Abs.hold @02:34:18.80 SamplePump.in :start @02:34:18.96 SamplePump.seek 1000ml,1:07:10 @02:34:19.49 SamplePump.configure :sample @02:34:21.91 filtering 2/1000ml at 0.84ml/s with 22.2psi vacuum @02:35:21.99 filtering 51/1000ml at 0.82ml/s with 24.6psi vacuum @02:36:21.97 filtering 99/1000ml at 0.80ml/s with 23.4psi vacuum @02:37:21.99 filtering 145/1000ml at 0.77ml/s with 24.1psi vacuum @02:38:21.98 filtering 190/1000ml at 0.75ml/s with 25.5psi vacuum @02:39:21.98 filtering 234/1000ml at 0.72ml/s with 23.6psi vacuum @02:40:21.97 filtering 276/1000ml at 0.70ml/s with 18.6psi vacuum @02:41:21.98 filtering 317/1000ml at 0.68ml/s with 22.9psi vacuum @02:42:21.97 filtering 356/1000ml at 0.66ml/s with 22.6psi vacuum @02:43:21.97 filtering 395/1000ml at 0.65ml/s with 21.2psi vacuum @02:44:21.97 filtering 433/1000ml at 0.63ml/s with 23.7psi vacuum @02:45:21.98 filtering 470/1000ml at 0.62ml/s with 22.8psi vacuum @02:46:22.01 filtering 506/1000ml at 0.60ml/s with 21.8psi vacuum @02:47:21.97 filtering 541/1000ml at 0.58ml/s with 31.7psi vacuum @02:48:21.97 filtering 575/1000ml at 0.57ml/s with 23.1psi vacuum @02:49:21.97 filtering 608/1000ml at 0.55ml/s with 27.3psi vacuum @02:50:21.98 filtering 640/1000ml at 0.54ml/s with 16.4psi vacuum @02:51:21.98 filtering 672/1000ml at 0.53ml/s with 23.3psi vacuum @02:52:21.98 filtering 703/1000ml at 0.52ml/s with 23.6psi vacuum @02:53:21.99 filtering 733/1000ml at 0.51ml/s with 21.8psi vacuum @02:54:21.97 filtering 763/1000ml at 0.50ml/s with 24.3psi vacuum @02:55:21.98 filtering 792/1000ml at 0.49ml/s with 21.5psi vacuum @02:56:22.73 filtering 821/1000ml at 0.48ml/s with 22.7psi vacuum @02:57:21.96 filtering 849/1000ml at 0.47ml/s with 27.2psi vacuum @02:58:21.98 filtering 876/1000ml at 0.46ml/s with 20.1psi vacuum @02:59:21.97 filtering 903/1000ml at 0.45ml/s with 26.9psi vacuum @03:00:21.99 filtering 930/1000ml at 0.44ml/s with 22.7psi vacuum @03:01:21.98 filtering 956/1000ml at 0.44ml/s with 24.2psi vacuum @03:02:21.98 filtering 982/1000ml at 0.43ml/s with 22.5psi vacuum @03:03:04.52 Abs.stop @03:03:04.66 Intake.close @03:03:04.70 Exhaust.close @03:03:04.95 Toroid.seek :bypassed @03:03:09.37 Sampled 1000.0ml @03:03:09.42 Toroid.seek :bypassed @03:03:09.58 delay 5 seconds @03:03:14.61 Handoff.select :waste @03:03:14.80 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:03:21.45 Syringe tare is 53% of full scale at 22C (drifted 2%) @03:03:21.49 PinPort.in :locking @03:03:21.64 PinPort.seek :locked @03:03:22.09 Archive.in :fast @03:03:22.26 Lysis2.in :fast @03:03:22.30 Lysis1.seek 1250uL @03:03:22.37 Archive.seek :empty @03:03:22.66 Lysis2.seek :empty @03:03:31.04 Evacuating puck with filter bubble point of 50psi @03:03:31.09 PinPort.seek :locked @03:03:31.26 Handoff.select :waste @03:03:31.33 Plunge.seek :puckBottom @03:03:32.72 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:03:39.34 Air syringe pumping up to 1 strokes to reach 8psi @03:03:39.36 Air stroke #1 of 1 @03:03:39.54 Air.in :pressurize @03:03:39.65 Air.seek :pressurized @03:03:45.04 0.2psi after pressurize @03:03:45.07 Air.in :compress @03:03:45.21 Air.seek :evac @03:03:52.87 0.9psi after compress @03:04:03.50 0.1psi after compress (stable) @03:04:03.54 Air.in :default @03:04:03.67 Air.hold @03:04:03.96 at 0.1psi Opened to waste @03:04:03.98 Handoff.select :blocked @03:04:04.21 Plunge.seek :puckTop @03:04:05.13 Air syringe pumping up to 8 strokes to reach 50psi @03:04:05.15 Air stroke #1 of 8 @03:04:05.33 Air.in :compress @03:04:05.43 Air.seek :evac @03:04:05.64 14.1psi after compress @03:04:16.29 14.4psi after compress (stable) @03:04:16.34 Air.in :gulp @03:04:16.44 Air.seek :topVent @03:04:23.20 14.3psi after gulp @03:04:28.82 14.3psi after gulp (stable) @03:04:28.83 Air stroke #2 of 8 @03:04:29.04 Air.in :pressurize @03:04:29.14 Air.seek :pressurized @03:04:35.54 14.2psi after pressurize @03:04:35.58 Air.in :compress @03:04:35.73 Air.seek :evac @03:04:43.36 32.6psi after compress @03:04:54.27 34.5psi after compress (stable) @03:04:54.42 Air.in :gulp @03:04:54.54 Air.seek :topVent @03:05:01.24 34.7psi after gulp @03:05:07.35 34.6psi after gulp (stable) @03:05:07.39 Air stroke #3 of 8 @03:05:07.57 Air.in :pressurize @03:05:07.67 Air.seek :pressurized @03:05:14.07 34.4psi after pressurize @03:05:14.13 Air.in :compress @03:05:14.32 Air.seek :evac @03:05:22.09 Reached 50.8 of 50.0psi after pumping 5432uL (3.00 strokes) @03:05:22.12 Air.in :default @03:05:22.25 Air.hold @03:05:22.38 Plunge.seek :puckBottom @03:05:23.30 delay 3 seconds @03:05:26.34 Handoff.select :waste @03:05:26.52 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:05:39.62 delay 1:00 @03:06:39.68 Handoff.select :blocked @03:06:39.85 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @03:07:13.44 Handoff.select :waste @03:07:13.62 5.8psi at evacEnd @03:07:13.63 Handoff.select :blocked @03:07:13.84 Plunge.seek :puckTop @03:07:14.76 Air.in :fast @03:07:14.91 Air.seek :topVent @03:07:21.64 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @03:07:28.25 Stable @5.9psi after Handoff blocked @03:07:28.30 Air syringe pumping up to 8 strokes to reach 35psi @03:07:28.32 Air stroke #1 of 8 @03:07:28.50 Air.in :pressurize @03:07:28.61 Air.seek :pressurized @03:07:35.00 6.1psi after pressurize @03:07:35.04 Air.in :compress @03:07:35.18 Air.seek :evac @03:07:42.83 19.9psi after compress @03:07:53.46 21.3psi after compress (stable) @03:07:53.50 Air.in :gulp @03:07:53.62 Air.seek :topVent @03:08:00.31 21.4psi after gulp @03:08:06.41 21.3psi after gulp (stable) @03:08:06.42 Air stroke #2 of 8 @03:08:06.61 Air.in :pressurize @03:08:06.72 Air.seek :pressurized @03:08:13.11 21.3psi after pressurize @03:08:13.15 Air.in :compress @03:08:13.27 Air.seek :evac @03:08:20.96 33.7psi after compress @03:08:22.75 Reached 35.1 of 35.0psi after pumping 3621uL (2.00 strokes) @03:08:22.77 Air.in :default @03:08:22.92 Air.hold @03:08:23.08 Plunge.seek :puckBottom @03:08:24.03 delay 3 seconds @03:08:27.05 Handoff.select :waste @03:08:27.25 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:08:33.85 delay 1:00 @03:09:33.94 Handoff.select :blocked @03:09:34.12 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @03:10:00.25 Handoff.select :waste @03:10:00.50 3.6psi at evacEnd @03:10:00.55 Plunge.seek :pTop @03:10:01.49 delay 5 seconds @03:10:06.52 Air.in :glide @03:10:06.63 Air.seek :fullVent @03:10:07.88 delay 2 seconds @03:10:09.91 Plunge.seek :pBottom @03:10:10.80 Seawater evacuated, Pressure above/below filter released. @03:10:10.84 Plunge.seek :locked @03:10:12.42 Handoff.select :waste @03:10:12.78 Lysis1.in :slow @03:10:12.97 Lysis1.seek 950uL @03:10:27.13 delay 5 seconds @03:10:32.16 Handoff.select :blocked @03:10:32.33 Plunge.seek :puckTop @03:10:33.41 Lysis1.seek 350uL @03:11:01.44 delay 3 seconds @03:11:04.56 Air.in mix with 5.31227 parts Lysis1 @03:11:05.68 Air.seek :evac @03:11:05.79 Lysis1.seek 190uL @03:11:13.49 Air.in :fast @03:11:13.62 Air.seek :full @03:11:20.32 Lysis1.seek :empty @03:11:29.50 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @27.7psi @03:11:29.54 Plunge.seek :puckBottom @03:11:30.62 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @24.3psi @03:11:30.65 Polling Can every 2:00 @03:11:31.26 PuckHeater.configure :default #ambient 21.5C @03:11:31.36 PuckHeater.seek 70.5C @03:12:47.26 PuckHeater.hold 2:00,70.5C @03:14:47.36 Polling Can every 2:00 @03:14:47.93 PuckHeater.configure :default #ambient 21.6C @03:14:48.03 PuckHeater.seek 69.4C @03:14:48.27 PuckHeater.hold 2:00,69.4C @03:16:48.31 Polling Can every 2:00 @03:16:48.92 PuckHeater.configure :default #ambient 21.5C @03:16:49.03 PuckHeater.hold 18:00,68.3C @03:34:48.09 Polling Can every 2:00 @03:34:48.65 PuckHeater.configure :default #ambient 21.5C @03:34:48.76 PuckHeater.seek 104.3C @03:35:51.64 PuckHeater.hold 2:00,104.3C @03:37:51.83 Polling Can every 2:00 @03:37:52.39 PuckHeater.configure :default #ambient 21.6C @03:37:52.49 PuckHeater.seek 103.3C @03:37:52.74 PuckHeater.hold 2:00,103.3C @03:39:52.84 Polling Can every 2:00 @03:39:53.41 PuckHeater.configure :default #ambient 21.6C @03:39:53.53 PuckHeater.hold 2:00,102.2C @03:41:53.72 delay 5:00 @03:46:53.82 Lysate cooked @14.7psi @03:46:53.86 Plunge.seek :puckTop @03:46:54.80 Air syringe pumping up to 5 strokes to reach 40psi @03:46:54.82 Air stroke #1 of 5 @03:46:55.02 Air.in :pressurize @03:46:55.98 Air.seek :pressurized @03:47:02.37 14.6psi after pressurize @03:47:02.42 Air.in :compress @03:47:02.55 Air.seek :evac @03:47:07.98 Reached 41.6 of 40.0psi after pumping 1682uL (0.93 strokes) @03:47:08.01 Air.in :default @03:47:08.13 Air.hold @03:47:08.27 delay 2 seconds @03:47:10.30 Air.in :fast @03:47:10.41 Air.seek :full @03:47:16.65 Plunge.seek :puckBottom @03:47:17.70 Lysis1.seek 138uL @03:47:24.30 delay 2 seconds @03:47:26.33 Plunge.seek :puckTop @03:47:27.27 Lysis1.seek :empty @03:47:33.90 Plunge.seek :puckBottom @03:47:35.06 Lysis1.in :extract @03:47:35.15 Lysis1.seek 675uL,nil @03:47:37.79 Air.in :pressurize @03:47:37.91 Air.seek :pressurized @03:47:44.29 11.6psi after pressurize @03:47:44.34 Air.in :compressLysate @03:47:44.47 Air.seek :evac @03:48:00.59 Reached 16.3 of 15.5psi after pumping 1613uL (0.89 strokes) @03:48:00.63 Air.in :default @03:48:00.75 Air.hold @03:48:01.83 Air.in :compressLysate @03:48:01.93 Air.seek :evac @03:48:12.76 Air.in :default @03:48:13.60 Air.hold @03:48:14.10 Pulled 675uL lysate @14.9psi @03:48:14.37 Lysis1.seek 800uL,nil @03:48:14.67 Air.in :compressLysate @03:48:14.86 Air.seek :evac @03:48:17.30 13.9psi after compress @03:48:32.01 13.9psi after compress (stable) @03:48:37.26 Air.in :default @03:48:37.45 Air.hold @03:48:37.58 Lysis1.in :default @03:48:37.76 Lysis1.hold @03:48:38.05 Air.in :glide @03:48:38.15 Air.seek :fullVent @03:48:39.41 Handoff.select :waste @03:48:39.58 Recovered 692uL lysate @14.0psi @03:48:39.60 delay 3 seconds @03:48:42.72 After 3 delay Recovered 692uL lysate @-0.9psi @03:48:42.74 delay 5 seconds @03:48:47.82 Gate.power :heat,:OFF @03:48:47.86 Gate.power :cartridge,:OFF @03:48:48.02 PinPort.in :unlocking @03:48:48.12 PinPort.seek :clear @03:48:48.64 Archive.in :unlocking @03:48:48.82 Archive.seek :clear @03:48:49.01 Air.in :unlocking @03:48:49.23 Air.seek :clear @03:48:49.33 Lysis2.in :unlocking @03:48:49.49 Lysis2.seek :clear @03:48:54.27 Lysis1.in :unlocking @03:48:54.49 Lysis1.seek :clear @03:48:54.58 Plunge.in :unlocking @03:48:54.73 Plunge.seek :clear @03:49:19.86 -> cleanPortable @03:49:20.41 Waiting for nadia to answer "LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue" @03:49:26.58 Answered LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue"" @03:49:26.71 Lysis1.in :fast @03:49:26.81 Lysis1.seek :locked @03:49:29.46 Plunge.in :default @03:49:29.60 Plunge.seek :locked @03:49:29.81 Air.in :fast @03:49:29.91 Air.seek :locked @03:49:30.97 Powering cartridge bus @03:49:33.69 Gate.power :cartridge,:OFF @03:49:34.26 Mounted archive Cartridge @03:49:34.34 Toroid.seek :bypassed @03:49:34.47 Priming sample loop w/10ml, bypass w/10ml @03:49:34.49 SamplePump.setPosition! 0ml @03:49:34.59 Bypass.open @03:49:36.25 Intake.open @03:49:37.28 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @03:49:39.91 Vacuum tare is 54% of full scale at 22C (drifted 2%) @03:49:40.13 Abs.in :default @03:49:40.24 Abs.hold @03:49:40.57 SamplePump.in :start @03:49:40.69 SamplePump.seek 10ml,1:10 @03:49:41.27 SamplePump.configure :prime @03:49:53.02 Abs.stop @03:49:53.12 SamplePump.setPosition! 0ml @03:49:53.22 Exhaust.open @03:49:54.83 Bypass.close @03:49:56.65 Abs.in :default @03:49:56.75 Abs.hold @03:49:57.20 SamplePump.in :start @03:49:57.31 SamplePump.seek 10ml,1:10 @03:49:57.88 SamplePump.configure :prime @03:50:09.61 Abs.stop @03:50:09.77 Waiting for nadia to answer "INTAKE line plumbed to 10% Bleach, push return to continue" @03:50:17.67 Answered INTAKE line plumbed to 10% Bleach, push return to continue"" @03:50:17.70 Toroid.seek :bypassed @03:50:17.85 Priming sample loop w/10ml, bypass w/10ml @03:50:17.87 SamplePump.setPosition! 0ml @03:50:17.97 Exhaust.close @03:50:18.85 Bypass.open @03:50:20.84 Abs.in :default @03:50:20.98 Abs.hold @03:50:21.31 SamplePump.in :start @03:50:21.42 SamplePump.seek 10ml,1:10 @03:50:21.99 SamplePump.configure :prime @03:50:33.73 Abs.stop @03:50:33.82 SamplePump.setPosition! 0ml @03:50:33.92 Exhaust.open @03:50:35.50 Bypass.close @03:50:37.25 Abs.in :default @03:50:37.32 Abs.hold @03:50:37.75 SamplePump.in :start @03:50:37.86 SamplePump.seek 10ml,1:10 @03:50:38.44 SamplePump.configure :prime @03:50:51.10 Abs.stop @03:50:51.24 Toroid.seek :cartridge @03:50:55.60 Sampling 25.0ml @03:50:55.62 SamplePump.setPosition! 0ml @03:50:55.96 Abs.in :default @03:50:56.05 Abs.hold @03:50:56.48 SamplePump.in :start @03:50:56.60 SamplePump.seek 25ml,2:10 @03:50:57.16 SamplePump.configure :sample @03:51:26.66 Abs.stop @03:51:26.74 Intake.close @03:51:26.85 Exhaust.close @03:51:27.03 Handoff.select :close @03:51:27.19 Toroid.seek :bypassed @03:51:31.61 Sampled 25.0ml @03:51:31.64 delay 10:00 @04:01:31.69 Waiting for nadia to answer "Move INTAKE line to MillQ water" @04:01:31.73 Answered Move INTAKE line to MillQ water"" @04:01:31.77 Toroid.seek :bypassed @04:01:31.94 Priming sample loop w/50ml, bypass w/25ml @04:01:31.96 SamplePump.setPosition! 0ml @04:01:32.07 Bypass.open @04:01:33.86 Intake.open @04:01:35.63 Abs.in :default @04:01:35.73 Abs.hold @04:01:36.04 SamplePump.in :start @04:01:36.14 SamplePump.seek 25ml,2:10 @04:01:36.73 SamplePump.configure :prime @04:02:06.21 Abs.stop @04:02:06.32 SamplePump.setPosition! 0ml @04:02:06.44 Exhaust.open @04:02:08.02 Bypass.close @04:02:09.79 Abs.hold @04:02:10.20 SamplePump.in :start @04:02:10.30 SamplePump.seek 50ml,3:50 @04:02:10.90 SamplePump.configure :prime @04:03:09.97 Abs.stop @04:03:10.06 Toroid.seek :cartridge @04:03:14.45 Sampling 25.0ml @04:03:14.47 SamplePump.setPosition! 0ml @04:03:14.81 Abs.in :default @04:03:14.95 Abs.hold @04:03:16.20 SamplePump.in :start @04:03:16.32 SamplePump.seek 25ml,2:35 @04:03:16.90 SamplePump.configure :sample @04:03:46.40 Abs.stop @04:03:46.53 Intake.close @04:03:46.63 Exhaust.close @04:03:46.72 Toroid.seek :bypassed @04:03:51.21 Sampled 25.0ml @04:03:51.57 Air.in :unlocking @04:03:51.75 Air.seek :clear @04:03:52.79 Lysis1.in :unlocking @04:03:53.09 Lysis1.seek :clear @04:03:53.17 Plunge.in :unlocking @04:03:53.37 Plunge.seek :clear @04:03:54.53 Portable sample Path clean, ready for next sample @04:04:08.32 -> Cart.fire @04:04:08.46 Lysis1.in :fast @04:04:08.57 Lysis1.seek :locked @04:04:11.18 Plunge.in :default @04:04:11.32 Plunge.seek :locked @04:04:11.52 Air.in :fast @04:04:11.60 Air.seek :locked @04:04:12.61 Powering cartridge bus @04:04:15.36 PuckHeater.configure :default #ambient 21.6C @04:04:15.46 Gate.power :heat,:ON,limit to 5000mA @04:04:16.19 Mounted lysis Cartridge board#J59945 @04:04:16.24 Using default cartridge heat loss curve for HeatingFunctionInStage @04:04:16.59 Air.in :default @04:04:16.63 Toroid.seek :bypassed @04:04:16.83 Air.seek :sealed @04:04:17.02 Priming sample loop w/25ml, bypass w/10ml @04:04:17.05 SamplePump.setPosition! 0ml @04:04:17.17 Bypass.open @04:04:18.89 Intake.open @04:04:19.89 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @04:04:20.45 Intake.close @04:04:20.57 Bypass.close @04:04:20.80 Toroid.seek :bypassed @04:04:21.64 UserAbort in nadia -- User Abort!! @04:04:50.79 -> Cart.fire @04:04:50.87 Gate.power :heat,:OFF @04:04:50.91 Gate.power :cartridge,:OFF @04:04:52.52 Powering cartridge bus @04:04:56.11 PuckHeater.configure :default #ambient 21.6C @04:04:56.23 Gate.power :heat,:ON,limit to 5000mA @04:04:57.01 Mounted lysis Cartridge board#J59945 @04:04:57.09 Using default cartridge heat loss curve for HeatingFunctionInStage @04:04:57.39 Air.seek :sealed @04:04:57.51 Toroid.seek :bypassed @04:04:57.72 Priming sample loop w/25ml, bypass w/10ml @04:04:57.74 SamplePump.setPosition! 0ml @04:04:57.85 Bypass.open @04:04:59.49 Intake.open @04:05:00.47 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @04:05:03.11 Vacuum tare is 54% of full scale at 22C (drifted 2%) @04:05:03.35 Abs.in :default @04:05:03.46 Abs.hold @04:05:03.78 SamplePump.in :start @04:05:03.89 SamplePump.seek 10ml,1:10 @04:05:04.47 SamplePump.configure :prime @04:05:16.17 Abs.stop @04:05:16.28 SamplePump.setPosition! 0ml @04:05:16.40 Exhaust.open @04:05:17.99 Bypass.close @04:05:19.79 Abs.in :default @04:05:19.89 Abs.hold @04:05:20.37 SamplePump.in :start @04:05:20.49 SamplePump.seek 25ml,2:10 @04:05:21.06 SamplePump.configure :prime @04:05:50.56 Abs.stop @04:05:50.66 Bypass.open @04:05:51.49 Intake.close @04:05:52.42 Toroid.seek :crack @04:05:56.74 Toroid.seek :cartridge @04:05:57.93 Pressurizing cartridge to 26psi above 13.8psia @04:05:57.96 SamplePump.setPosition! 0ml @04:05:58.08 Intake.open @04:05:59.03 Bypass.close @04:06:00.73 Exhaust.close @04:06:01.80 Abs.in :default @04:06:01.90 Abs.hold @04:06:02.23 SamplePump.in :charge @04:06:02.35 SamplePump.seek 2.3ml,40 seconds @04:06:19.76 Abs.stop @04:06:19.88 Intake.close @04:06:20.84 Pressurized cartridge with 1.388ml to 26.2psi @04:06:20.86 Isolated @38.8psia (25.0psi) @04:06:20.96 Verifying cartridge holds at least 0.65..0.85 of 38.8psia @04:06:21.04 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @04:06:25.66 Cartridge pressure fell 1% to 38.5psia @04:06:25.71 Toroid.seek :cartridge @04:06:25.89 Sampling 1000.0ml @04:06:25.91 SamplePump.setPosition! 0ml @04:06:26.03 Exhaust.open @04:06:26.86 Intake.open @04:06:28.04 Abs.in :default @04:06:28.13 Abs.hold @04:06:28.55 SamplePump.in :start @04:06:28.67 SamplePump.seek 1000ml,1:07:10 @04:06:29.24 SamplePump.configure :sample @04:06:31.67 filtering 2/1000ml at 0.81ml/s with 23.2psi vacuum @04:07:31.73 filtering 51/1000ml at 0.81ml/s with 21.5psi vacuum @04:08:31.72 filtering 99/1000ml at 0.81ml/s with 19.5psi vacuum @04:09:31.72 filtering 147/1000ml at 0.79ml/s with 24.3psi vacuum @04:10:31.71 filtering 193/1000ml at 0.77ml/s with 22.3psi vacuum @04:11:31.71 filtering 238/1000ml at 0.76ml/s with 25.2psi vacuum @04:12:31.73 filtering 282/1000ml at 0.74ml/s with 22.0psi vacuum @04:13:31.72 filtering 326/1000ml at 0.72ml/s with 18.9psi vacuum @04:14:31.72 filtering 369/1000ml at 0.71ml/s with 24.6psi vacuum @04:15:31.72 filtering 410/1000ml at 0.69ml/s with 27.1psi vacuum @04:16:31.71 filtering 451/1000ml at 0.68ml/s with 21.2psi vacuum @04:17:32.55 filtering 491/1000ml at 0.67ml/s with 25.1psi vacuum @04:18:31.74 filtering 530/1000ml at 0.64ml/s with 27.6psi vacuum @04:19:31.72 filtering 568/1000ml at 0.64ml/s with 21.8psi vacuum @04:20:31.72 filtering 606/1000ml at 0.63ml/s with 24.6psi vacuum @04:21:31.72 filtering 644/1000ml at 0.63ml/s with 25.0psi vacuum @04:22:31.73 filtering 681/1000ml at 0.62ml/s with 24.6psi vacuum @04:23:31.76 filtering 717/1000ml at 0.61ml/s with 19.5psi vacuum @04:24:31.72 filtering 753/1000ml at 0.60ml/s with 23.4psi vacuum @04:25:31.73 filtering 788/1000ml at 0.59ml/s with 25.0psi vacuum @04:26:31.71 filtering 823/1000ml at 0.58ml/s with 20.2psi vacuum @04:27:31.73 filtering 857/1000ml at 0.57ml/s with 22.2psi vacuum @04:28:31.73 filtering 891/1000ml at 0.56ml/s with 26.8psi vacuum @04:29:31.71 filtering 925/1000ml at 0.56ml/s with 23.3psi vacuum @04:30:31.73 filtering 958/1000ml at 0.55ml/s with 21.8psi vacuum @04:31:31.72 filtering 991/1000ml at 0.55ml/s with 22.7psi vacuum @04:31:48.40 Abs.stop @04:31:48.53 Intake.close @04:31:48.64 Exhaust.close @04:31:48.77 Toroid.seek :bypassed @04:31:53.31 Sampled 1000.0ml @04:31:53.42 Toroid.seek :bypassed @04:31:53.57 delay 5 seconds @04:31:58.59 Handoff.select :waste @04:31:58.76 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @04:32:05.42 Syringe tare is 53% of full scale at 22C (drifted 2%) @04:32:05.47 PinPort.in :locking @04:32:05.61 PinPort.seek :locked @04:32:06.06 Archive.in :fast @04:32:06.22 Lysis2.in :fast @04:32:06.27 Lysis1.seek 1250uL @04:32:06.40 Archive.seek :empty @04:32:06.52 Lysis2.seek :empty @04:32:15.00 Evacuating puck with filter bubble point of 50psi @04:32:15.87 PinPort.seek :locked @04:32:16.03 Handoff.select :waste @04:32:16.10 Plunge.seek :puckBottom @04:32:17.55 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @04:32:24.17 Air syringe pumping up to 1 strokes to reach 8psi @04:32:24.19 Air stroke #1 of 1 @04:32:24.39 Air.in :pressurize @04:32:24.51 Air.seek :pressurized @04:32:29.91 0.2psi after pressurize @04:32:29.96 Air.in :compress @04:32:30.09 Air.seek :evac @04:32:37.73 -0.1psi after compress @04:32:48.37 0.1psi after compress (stable) @04:32:48.39 Air.in :default @04:32:48.51 Air.hold @04:32:48.82 at 0.0psi Opened to waste @04:32:48.84 Handoff.select :blocked @04:32:49.08 Plunge.seek :puckTop @04:32:50.02 Air syringe pumping up to 8 strokes to reach 50psi @04:32:50.05 Air stroke #1 of 8 @04:32:50.23 Air.in :compress @04:32:50.34 Air.seek :evac @04:32:51.41 13.9psi after compress @04:33:02.03 13.7psi after compress (stable) @04:33:02.08 Air.in :gulp @04:33:02.21 Air.seek :topVent @04:33:08.89 13.8psi after gulp @04:33:14.55 13.8psi after gulp (stable) @04:33:14.57 Air stroke #2 of 8 @04:33:14.72 Air.in :pressurize @04:33:14.83 Air.seek :pressurized @04:33:21.27 13.6psi after pressurize @04:33:21.30 Air.in :compress @04:33:21.43 Air.seek :evac @04:33:29.08 32.4psi after compress @04:33:39.68 34.4psi after compress (stable) @04:33:39.80 Air.in :gulp @04:33:39.91 Air.seek :topVent @04:33:46.60 34.4psi after gulp @04:33:52.70 34.3psi after gulp (stable) @04:33:52.74 Air stroke #3 of 8 @04:33:52.97 Air.in :pressurize @04:33:53.07 Air.seek :pressurized @04:33:59.48 34.3psi after pressurize @04:33:59.50 Air.in :compress @04:33:59.61 Air.seek :evac @04:34:07.41 Reached 50.8 of 50.0psi after pumping 5432uL (3.00 strokes) @04:34:07.43 Air.in :default @04:34:07.55 Air.hold @04:34:07.71 Plunge.seek :puckBottom @04:34:08.63 delay 3 seconds @04:34:11.66 Handoff.select :waste @04:34:11.86 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @04:34:25.98 delay 1:00 @04:35:26.01 Handoff.select :blocked @04:35:26.17 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @04:35:48.78 Handoff.select :waste @04:35:49.00 2.5psi at evacEnd @04:35:49.01 Handoff.select :blocked @04:35:49.22 Plunge.seek :puckTop @04:35:50.14 Air.in :fast @04:35:50.25 Air.seek :topVent @04:35:56.98 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @04:36:03.58 Stable @24.1psi after Handoff blocked @04:36:03.63 Air syringe pumping up to 8 strokes to reach 35psi @04:36:03.65 Air stroke #1 of 8 @04:36:03.83 Air.in :pressurize @04:36:03.94 Air.seek :pressurized @04:36:10.32 24.1psi after pressurize @04:36:10.38 Air.in :compress @04:36:10.50 Air.seek :evac @04:36:17.79 Reached 35.4 of 35.0psi after pumping 1790uL (0.99 strokes) @04:36:17.82 Air.in :default @04:36:17.95 Air.hold @04:36:18.08 Plunge.seek :puckBottom @04:36:19.03 delay 3 seconds @04:36:22.05 Handoff.select :waste @04:36:22.24 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @04:36:28.84 delay 1:00 @04:37:28.94 Handoff.select :blocked @04:37:29.14 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @04:37:51.80 Handoff.select :waste @04:37:52.79 0.1psi at evacEnd @04:37:52.86 Plunge.seek :pTop @04:37:53.85 delay 5 seconds @04:37:58.91 Air.in :glide @04:37:59.02 Air.seek :fullVent @04:38:00.63 delay 2 seconds @04:38:02.67 Plunge.seek :pBottom @04:38:03.58 Seawater evacuated, Pressure above/below filter released. @04:38:03.61 Plunge.seek :locked @04:38:05.03 Handoff.select :waste @04:38:05.21 Lysis1.in :slow @04:38:05.31 Lysis1.seek 950uL @04:38:19.41 delay 5 seconds @04:38:24.44 Handoff.select :blocked @04:38:24.65 Plunge.seek :puckTop @04:38:25.71 Lysis1.seek 350uL @04:38:53.75 delay 3 seconds @04:38:56.87 Air.in mix with 5.31227 parts Lysis1 @04:38:57.97 Air.seek :evac @04:38:58.08 Lysis1.seek 190uL @04:39:05.80 Air.in :fast @04:39:05.92 Air.seek :full @04:39:12.65 Lysis1.seek :empty @04:39:21.83 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @12.8psi @04:39:21.87 Plunge.seek :puckBottom @04:39:22.99 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @11.4psi @04:39:23.02 Polling Can every 2:00 @04:39:23.62 PuckHeater.configure :default #ambient 21.6C @04:39:23.71 PuckHeater.seek 70.5C @04:40:34.67 PuckHeater.hold 2:00,70.5C @04:42:34.71 Polling Can every 2:00 @04:42:35.27 PuckHeater.configure :default #ambient 21.6C @04:42:35.37 PuckHeater.seek 69.4C @04:42:35.62 PuckHeater.hold 2:00,69.4C @04:44:35.57 Polling Can every 2:00 @04:44:36.13 PuckHeater.configure :default #ambient 21.6C @04:44:36.23 PuckHeater.hold 18:00,68.3C @05:02:34.61 Polling Can every 2:00 @05:02:35.17 PuckHeater.configure :default #ambient 21.5C @05:02:35.27 PuckHeater.seek 104.3C @05:03:34.90 PuckHeater.hold 2:00,104.3C @05:05:34.96 Polling Can every 2:00 @05:05:35.54 PuckHeater.configure :default #ambient 21.6C @05:05:35.64 PuckHeater.seek 103.3C @05:05:35.90 PuckHeater.hold 2:00,103.3C @05:07:35.81 Polling Can every 2:00 @05:07:36.38 PuckHeater.configure :default #ambient 21.6C @05:07:36.49 PuckHeater.hold 2:00,102.2C @05:09:36.52 delay 5:00 @05:14:36.60 Lysate cooked @4.3psi @05:14:36.63 Plunge.seek :puckTop @05:14:37.59 Air syringe pumping up to 5 strokes to reach 40psi @05:14:37.61 Air stroke #1 of 5 @05:14:37.76 Air.in :pressurize @05:14:37.85 Air.seek :pressurized @05:14:44.25 5.0psi after pressurize @05:14:44.28 Air.in :compress @05:14:44.41 Air.seek :evac @05:14:50.28 Reached 41.4 of 40.0psi after pumping 1706uL (0.94 strokes) @05:14:50.33 Air.in :default @05:14:50.47 Air.hold @05:14:51.51 delay 2 seconds @05:14:53.57 Air.in :fast @05:14:53.67 Air.seek :full @05:15:00.04 Plunge.seek :puckBottom @05:15:01.05 Lysis1.seek 138uL @05:15:07.66 delay 2 seconds @05:15:09.70 Plunge.seek :puckTop @05:15:10.61 Lysis1.seek :empty @05:15:17.23 Plunge.seek :puckBottom @05:15:18.35 Lysis1.in :extract @05:15:18.54 Lysis1.seek 675uL,nil @05:15:21.05 Air.in :pressurize @05:15:21.16 Air.seek :pressurized @05:15:27.57 11.7psi after pressurize @05:15:27.60 Air.in :compressLysate @05:15:28.53 Air.seek :evac @05:15:50.98 Air.in :default @05:15:51.15 Air.hold @05:15:51.47 Pulled 675uL lysate @15.5psi @05:15:51.76 Lysis1.seek 800uL,nil @05:15:52.59 Air.in :compressLysate @05:15:52.73 Air.seek :evac @05:16:00.18 14.1psi after compress @05:16:10.99 14.5psi after compress (stable) @05:16:16.47 Air.in :default @05:16:16.56 Lysis1.in :default @05:16:16.60 Air.hold @05:16:16.79 Lysis1.hold @05:16:17.11 Air.in :glide @05:16:17.21 Air.seek :fullVent @05:16:18.47 Handoff.select :waste @05:16:18.63 Recovered 714uL lysate @14.5psi @05:16:18.65 delay 3 seconds @05:16:21.79 After 3 delay Recovered 714uL lysate @-2.0psi @05:16:21.81 delay 5 seconds @05:16:26.86 Gate.power :heat,:OFF @05:16:26.90 Gate.power :cartridge,:OFF @05:16:27.07 PinPort.in :unlocking @05:16:27.17 PinPort.seek :clear @05:16:27.69 Archive.in :unlocking @05:16:27.83 Archive.seek :clear @05:16:28.03 Air.in :unlocking @05:16:28.22 Air.seek :clear @05:16:28.34 Lysis2.in :unlocking @05:16:28.46 Lysis2.seek :clear @05:16:33.24 Lysis1.in :unlocking @05:16:33.42 Lysis1.seek :clear @05:16:33.59 Plunge.in :unlocking @05:16:33.67 Plunge.seek :clear @05:17:16.00 -> cleanPortable @05:17:17.52 Waiting for nadia to answer "LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue" @05:17:49.44 Answered LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue"" @05:17:49.60 Lysis1.in :fast @05:17:49.70 Lysis1.seek :locked @05:17:52.29 Plunge.in :default @05:17:52.46 Plunge.seek :locked @05:17:52.67 Air.in :fast @05:17:52.77 Air.seek :locked @05:17:53.80 Powering cartridge bus @05:17:56.55 Gate.power :cartridge,:OFF @05:17:57.12 Mounted archive Cartridge @05:17:57.19 Toroid.seek :bypassed @05:17:57.35 Priming sample loop w/10ml, bypass w/10ml @05:17:57.37 SamplePump.setPosition! 0ml @05:17:57.47 Bypass.open @05:17:59.14 Intake.open @05:18:00.13 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @05:18:02.78 Vacuum tare is 54% of full scale at 22C (drifted 2%) @05:18:03.05 Abs.in :default @05:18:03.14 Abs.hold @05:18:03.47 SamplePump.in :start @05:18:03.59 SamplePump.seek 10ml,1:10 @05:18:04.16 SamplePump.configure :prime @05:18:15.89 Abs.stop @05:18:15.98 SamplePump.setPosition! 0ml @05:18:16.07 Exhaust.open @05:18:17.70 Bypass.close @05:18:19.41 Abs.in :default @05:18:19.50 Abs.hold @05:18:19.89 SamplePump.in :start @05:18:20.02 SamplePump.seek 10ml,1:10 @05:18:20.59 SamplePump.configure :prime @05:18:32.33 Abs.stop @05:18:32.43 Waiting for nadia to answer "INTAKE line plumbed to 10% Bleach, push return to continue" @05:18:48.60 Answered INTAKE line plumbed to 10% Bleach, push return to continue"" @05:18:48.62 Toroid.seek :bypassed @05:18:48.79 Priming sample loop w/10ml, bypass w/10ml @05:18:48.81 SamplePump.setPosition! 0ml @05:18:48.96 Exhaust.close @05:18:49.81 Bypass.open @05:18:52.50 Abs.in :default @05:18:52.61 Abs.hold @05:18:52.99 SamplePump.in :start @05:18:53.11 SamplePump.seek 10ml,1:10 @05:18:53.68 SamplePump.configure :prime @05:19:05.43 Abs.stop @05:19:05.52 SamplePump.setPosition! 0ml @05:19:05.60 Exhaust.open @05:19:07.16 Bypass.close @05:19:08.92 Abs.in :default @05:19:09.04 Abs.hold @05:19:09.43 SamplePump.in :start @05:19:09.55 SamplePump.seek 10ml,1:10 @05:19:10.12 SamplePump.configure :prime @05:19:21.83 Abs.stop @05:19:21.95 Toroid.seek :cartridge @05:19:26.35 Sampling 25.0ml @05:19:26.37 SamplePump.setPosition! 0ml @05:19:26.70 Abs.in :default @05:19:26.79 Abs.hold @05:19:27.26 SamplePump.in :start @05:19:27.37 SamplePump.seek 25ml,2:10 @05:19:27.95 SamplePump.configure :sample @05:19:57.42 Abs.stop @05:19:57.50 Intake.close @05:19:57.60 Exhaust.close @05:19:57.73 Handoff.select :close @05:19:57.82 Toroid.seek :bypassed @05:20:02.32 Sampled 25.0ml @05:20:02.38 delay 10:00 @05:30:02.43 Waiting for nadia to answer "Move INTAKE line to MillQ water" @05:30:02.49 Answered Move INTAKE line to MillQ water"" @05:30:02.52 Toroid.seek :bypassed @05:30:02.70 Priming sample loop w/50ml, bypass w/25ml @05:30:02.76 SamplePump.setPosition! 0ml @05:30:02.84 Bypass.open @05:30:04.66 Intake.open @05:30:06.40 Abs.in :default @05:30:06.51 Abs.hold @05:30:06.83 SamplePump.in :start @05:30:06.96 SamplePump.seek 25ml,2:10 @05:30:07.91 SamplePump.configure :prime @05:30:37.70 Abs.stop @05:30:37.81 SamplePump.setPosition! 0ml @05:30:37.89 Exhaust.open @05:30:39.46 Bypass.close @05:30:41.24 Abs.in :default @05:30:41.33 Abs.hold @05:30:41.73 SamplePump.in :start @05:30:41.85 SamplePump.seek 50ml,3:50 @05:30:42.42 SamplePump.configure :prime @05:31:41.48 Abs.stop @05:31:41.57 Toroid.seek :cartridge @05:31:45.95 Sampling 25.0ml @05:31:45.97 SamplePump.setPosition! 0ml @05:31:46.30 Abs.in :default @05:31:46.40 Abs.hold @05:31:46.81 SamplePump.in :start @05:31:47.00 SamplePump.seek 25ml,2:35 @05:31:47.50 SamplePump.configure :sample @05:32:17.06 Abs.stop @05:32:17.17 Intake.close @05:32:17.29 Exhaust.close @05:32:17.40 Toroid.seek :bypassed @05:32:21.84 Sampled 25.0ml @05:32:22.17 Air.in :unlocking @05:32:22.34 Air.seek :clear @05:32:23.38 Lysis1.in :unlocking @05:32:23.60 Lysis1.seek :clear @05:32:23.65 Plunge.in :unlocking @05:32:23.85 Plunge.seek :clear @05:32:25.02 Portable sample Path clean, ready for next sample @05:36:34.23 -> Cart.fire @05:36:34.38 Lysis1.in :fast @05:36:34.48 Lysis1.seek :locked @05:36:37.07 Plunge.in :default @05:36:37.19 Plunge.seek :locked @05:36:37.43 Air.in :fast @05:36:37.52 Air.seek :locked @05:36:38.55 Powering cartridge bus @05:36:41.28 PuckHeater.configure :default #ambient 21.6C @05:36:42.24 Gate.power :heat,:ON,limit to 5000mA @05:36:43.01 Mounted lysis Cartridge board#J59927 @05:36:43.07 Using default cartridge heat loss curve for HeatingFunctionInStage @05:36:43.38 Air.in :default @05:36:43.47 Toroid.seek :bypassed @05:36:43.55 Air.seek :sealed @05:36:43.80 Priming sample loop w/25ml, bypass w/10ml @05:36:43.84 SamplePump.setPosition! 0ml @05:36:43.96 Bypass.open @05:36:45.59 Intake.open @05:36:46.63 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @05:36:49.29 Vacuum tare is 54% of full scale at 22C (drifted 2%) @05:36:49.57 Abs.in :default @05:36:49.66 Abs.hold @05:36:49.99 SamplePump.in :start @05:36:50.09 SamplePump.seek 10ml,1:10 @05:36:50.68 SamplePump.configure :prime @05:37:02.40 Abs.stop @05:37:02.50 SamplePump.setPosition! 0ml @05:37:02.58 Exhaust.open @05:37:04.18 Bypass.close @05:37:05.92 Abs.in :default @05:37:06.01 Abs.hold @05:37:06.40 SamplePump.in :start @05:37:06.52 SamplePump.seek 25ml,2:10 @05:37:07.10 SamplePump.configure :prime @05:37:36.63 Abs.stop @05:37:36.74 Bypass.open @05:37:37.62 Intake.close @05:37:38.50 Toroid.seek :crack @05:37:42.82 Toroid.seek :cartridge @05:37:44.03 Pressurizing cartridge to 26psi above 14.2psia @05:37:44.05 SamplePump.setPosition! 0ml @05:37:44.16 Intake.open @05:37:45.05 Bypass.close @05:37:45.92 Exhaust.close @05:37:47.79 Abs.in :default @05:37:47.89 Abs.hold @05:37:48.19 SamplePump.in :charge @05:37:48.30 SamplePump.seek 2.3ml,40 seconds @05:38:07.45 Abs.stop @05:38:07.58 Intake.close @05:38:08.56 Pressurized cartridge with 1.688ml to 26.5psi @05:38:08.60 Isolated @37.1psia (22.9psi) @05:38:08.64 Verifying cartridge holds at least 0.65..0.85 of 37.1psia @05:38:08.70 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @05:38:13.35 Cartridge pressure fell 1% to 36.9psia @05:38:13.38 Toroid.seek :cartridge @05:38:13.57 Sampling 1000.0ml @05:38:13.59 SamplePump.setPosition! 0ml @05:38:13.71 Exhaust.open @05:38:14.60 Intake.open @05:38:15.73 Abs.in :default @05:38:15.83 Abs.hold @05:38:16.26 SamplePump.in :start @05:38:16.37 SamplePump.seek 1000ml,1:07:10 @05:38:16.96 SamplePump.configure :sample @05:38:19.37 filtering 2/1000ml at 0.84ml/s with 21.3psi vacuum @05:39:19.42 filtering 52/1000ml at 0.83ml/s with 19.6psi vacuum @05:40:19.46 filtering 102/1000ml at 0.84ml/s with 22.5psi vacuum @05:41:19.45 filtering 153/1000ml at 0.84ml/s with 22.6psi vacuum @05:42:19.42 filtering 203/1000ml at 0.84ml/s with 21.5psi vacuum @05:43:19.41 filtering 253/1000ml at 0.84ml/s with 22.5psi vacuum @05:44:19.44 filtering 303/1000ml at 0.84ml/s with 21.0psi vacuum @05:45:19.41 filtering 353/1000ml at 0.83ml/s with 20.1psi vacuum @05:46:19.42 filtering 403/1000ml at 0.83ml/s with 23.5psi vacuum @05:47:19.42 filtering 453/1000ml at 0.83ml/s with 21.3psi vacuum @05:48:19.44 filtering 503/1000ml at 0.83ml/s with 23.2psi vacuum @05:49:20.26 filtering 552/1000ml at 0.83ml/s with 19.8psi vacuum @05:50:19.42 filtering 602/1000ml at 0.83ml/s with 19.3psi vacuum @05:51:19.43 filtering 651/1000ml at 0.82ml/s with 24.4psi vacuum @05:52:19.42 filtering 701/1000ml at 0.82ml/s with 20.7psi vacuum @05:53:19.42 filtering 750/1000ml at 0.82ml/s with 20.6psi vacuum @05:54:19.43 filtering 800/1000ml at 0.82ml/s with 23.4psi vacuum @05:55:19.43 filtering 849/1000ml at 0.82ml/s with 21.9psi vacuum @05:56:19.42 filtering 899/1000ml at 0.83ml/s with 21.1psi vacuum @05:57:19.44 filtering 948/1000ml at 0.83ml/s with 22.2psi vacuum @05:58:19.43 filtering 998/1000ml at 0.83ml/s with 22.2psi vacuum @05:58:22.11 Abs.stop @05:58:22.24 Intake.close @05:58:22.36 Exhaust.close @05:58:22.52 Toroid.seek :bypassed @05:58:26.91 Sampled 1000.0ml @05:58:27.00 Toroid.seek :bypassed @05:58:27.16 delay 5 seconds @05:58:32.19 Handoff.select :waste @05:58:32.38 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @05:58:39.06 Syringe tare is 52% of full scale at 22C (drifted 1%) @05:58:39.11 PinPort.in :locking @05:58:39.22 PinPort.seek :locked @05:58:39.69 Archive.in :fast @05:58:39.79 Lysis2.in :fast @05:58:39.87 Lysis1.seek 1250uL @05:58:40.11 Archive.seek :empty @05:58:40.30 Lysis2.seek :empty @05:58:48.69 Evacuating puck with filter bubble point of 50psi @05:58:48.76 PinPort.seek :locked @05:58:48.90 Handoff.select :waste @05:58:48.98 Plunge.seek :puckBottom @05:58:50.42 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @05:58:57.06 Air syringe pumping up to 1 strokes to reach 8psi @05:58:57.08 Air stroke #1 of 1 @05:58:57.24 Air.in :pressurize @05:58:57.34 Air.seek :pressurized @05:59:02.74 0.4psi after pressurize @05:59:02.78 Air.in :compress @05:59:02.91 Air.seek :evac @05:59:10.58 0.1psi after compress @05:59:21.22 -0.0psi after compress (stable) @05:59:21.25 Air.in :default @05:59:21.39 Air.hold @05:59:21.70 at 0.1psi Opened to waste @05:59:21.72 Handoff.select :blocked @05:59:21.92 Plunge.seek :puckTop @05:59:22.82 Air syringe pumping up to 8 strokes to reach 50psi @05:59:22.84 Air stroke #1 of 8 @05:59:23.04 Air.in :compress @05:59:23.15 Air.seek :evac @05:59:23.37 12.4psi after compress @05:59:33.97 12.6psi after compress (stable) @05:59:34.01 Air.in :gulp @05:59:34.16 Air.seek :topVent @05:59:40.84 12.5psi after gulp @05:59:46.48 12.6psi after gulp (stable) @05:59:46.50 Air stroke #2 of 8 @05:59:46.68 Air.in :pressurize @05:59:46.78 Air.seek :pressurized @05:59:53.17 12.5psi after pressurize @05:59:53.20 Air.in :compress @05:59:53.36 Air.seek :evac @06:00:01.03 30.6psi after compress @06:00:11.62 32.6psi after compress (stable) @06:00:11.76 Air.in :gulp @06:00:11.89 Air.seek :topVent @06:00:18.55 32.6psi after gulp @06:00:24.19 32.7psi after gulp (stable) @06:00:24.20 Air stroke #3 of 8 @06:00:24.38 Air.in :pressurize @06:00:24.49 Air.seek :pressurized @06:00:30.88 32.6psi after pressurize @06:00:30.96 Air.in :compress @06:00:31.10 Air.seek :evac @06:00:38.75 48.4psi after compress @06:00:41.81 Reached 50.2 of 50.0psi after pumping 5432uL (3.00 strokes) @06:00:41.83 Air.in :default @06:00:41.97 Air.hold @06:00:42.14 Plunge.seek :puckBottom @06:00:43.07 delay 3 seconds @06:00:46.10 Handoff.select :waste @06:00:46.28 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @06:00:56.88 delay 1:00 @06:01:56.97 Handoff.select :blocked @06:01:57.16 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @06:02:37.79 Handoff.select :waste @06:02:37.97 5.5psi at evacEnd @06:02:38.00 Handoff.select :blocked @06:02:38.21 Plunge.seek :puckTop @06:02:39.12 Air.in :fast @06:02:39.25 Air.seek :topVent @06:02:45.94 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @06:02:52.93 Stable @29.3psi after Handoff blocked @06:02:53.03 Air syringe pumping up to 8 strokes to reach 35psi @06:02:53.06 Air stroke #1 of 8 @06:02:53.28 Air.in :pressurize @06:02:53.38 Air.seek :pressurized @06:02:59.77 29.3psi after pressurize @06:02:59.82 Air.in :compress @06:02:59.95 Air.seek :evac @06:03:06.02 Reached 35.7 of 35.0psi after pumping 1720uL (0.95 strokes) @06:03:06.06 Air.in :default @06:03:06.20 Air.hold @06:03:06.33 Plunge.seek :puckBottom @06:03:07.27 delay 3 seconds @06:03:10.30 Handoff.select :waste @06:03:10.48 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @06:03:22.61 delay 1:00 @06:04:22.64 Handoff.select :blocked @06:04:22.86 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @06:04:45.47 Handoff.select :waste @06:04:45.66 0.7psi at evacEnd @06:04:45.69 Plunge.seek :pTop @06:04:46.63 delay 5 seconds @06:04:51.68 Air.in :glide @06:04:51.80 Air.seek :fullVent @06:04:54.21 delay 2 seconds @06:04:56.24 Plunge.seek :pBottom @06:04:57.24 Seawater evacuated, Pressure above/below filter released. @06:04:57.27 Plunge.seek :locked @06:04:58.71 Handoff.select :waste @06:04:58.88 Lysis1.in :slow @06:04:59.01 Lysis1.seek 950uL @06:05:13.10 delay 5 seconds @06:05:18.13 Handoff.select :blocked @06:05:18.33 Plunge.seek :puckTop @06:05:20.21 Lysis1.seek 350uL @06:05:48.25 delay 3 seconds @06:05:51.35 Air.in mix with 5.31227 parts Lysis1 @06:05:52.33 Air.seek :evac @06:05:52.41 Lysis1.seek 190uL @06:06:00.18 Air.in :fast @06:06:00.28 Air.seek :full @06:06:07.02 Lysis1.seek :empty @06:06:16.18 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @17.3psi @06:06:16.21 Plunge.seek :puckBottom @06:06:17.31 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @15.3psi @06:06:17.34 Polling Can every 2:00 @06:06:17.95 PuckHeater.configure :default #ambient 21.9C @06:06:18.04 PuckHeater.seek 70.4C @06:07:30.65 PuckHeater.hold 2:00,70.4C @06:09:30.57 Polling Can every 2:00 @06:09:31.14 PuckHeater.configure :default #ambient 21.9C @06:09:31.26 PuckHeater.seek 69.4C @06:09:31.52 PuckHeater.hold 2:00,69.4C @06:11:31.30 Polling Can every 2:00 @06:11:31.87 PuckHeater.configure :default #ambient 21.9C @06:11:31.97 PuckHeater.hold 18:00,68.3C @06:29:29.47 Polling Can every 2:00 @06:29:30.04 PuckHeater.configure :default #ambient 21.8C @06:29:30.15 PuckHeater.seek 104.3C @06:30:36.20 PuckHeater.hold 2:00,104.3C @06:32:36.05 Polling Can every 2:00 @06:32:36.62 PuckHeater.configure :default #ambient 21.9C @06:32:36.72 PuckHeater.seek 103.3C @06:32:37.03 PuckHeater.hold 2:00,103.3C @06:34:37.00 Polling Can every 2:00 @06:34:37.55 PuckHeater.configure :default #ambient 21.9C @06:34:37.68 PuckHeater.hold 2:00,102.2C @06:36:37.64 delay 5:00 @06:41:37.73 Lysate cooked @11.7psi @06:41:37.76 Plunge.seek :puckTop @06:41:38.72 Air syringe pumping up to 5 strokes to reach 40psi @06:41:38.73 Air stroke #1 of 5 @06:41:38.92 Air.in :pressurize @06:41:39.91 Air.seek :pressurized @06:41:46.33 12.5psi after pressurize @06:41:46.37 Air.in :compress @06:41:46.51 Air.seek :evac @06:41:51.81 Reached 41.5 of 40.0psi after pumping 1674uL (0.92 strokes) @06:41:51.83 Air.in :default @06:41:51.95 Air.hold @06:41:52.10 delay 2 seconds @06:41:54.14 Air.in :fast @06:41:54.23 Air.seek :full @06:42:00.43 Plunge.seek :puckBottom @06:42:01.46 Lysis1.seek 138uL @06:42:08.07 delay 2 seconds @06:42:10.12 Plunge.seek :puckTop @06:42:11.03 Lysis1.seek :empty @06:42:17.67 Plunge.seek :puckBottom @06:42:18.80 Lysis1.in :extract @06:42:18.99 Lysis1.seek 675uL,nil @06:42:21.45 Air.in :pressurize @06:42:21.57 Air.seek :pressurized @06:42:28.08 11.0psi after pressurize @06:42:28.15 Air.in :compressLysate @06:42:28.28 Air.seek :evac @06:42:42.88 Reached 15.8 of 15.5psi after pumping 1588uL (0.88 strokes) @06:42:43.02 Air.in :default @06:42:43.19 Air.hold @06:42:43.87 Air.in :compressLysate @06:42:43.99 Air.seek :evac @06:42:44.39 Syringe::LysatePlungerStuck in nadia -- at < 464uL during Lysis Syringe #1 (LyS1) move @06:42:44.45 Lysis1.in :default @06:42:44.55 Lysis1.seek 434uL @06:42:45.34 Reached 16.1 of 15.5psi after pumping 1604uL (0.89 strokes) @06:42:45.36 Air.in :default @06:42:45.48 Air.hold @06:42:45.79 Lysis1.in :extract @06:42:45.96 Lysis1.seek 675uL,nil @06:42:47.38 Air.in :compressLysate @06:42:47.51 Air.seek :evac @06:42:55.29 Air.in :default @06:42:55.41 Air.hold @06:42:55.81 Pulled 675uL lysate @16.1psi @06:42:56.06 Lysis1.seek 800uL,nil @06:42:57.47 Air.in :compressLysate @06:42:57.60 Air.seek :evac @06:43:04.75 13.2psi after compress @06:43:15.36 13.6psi after compress (stable) @06:43:20.57 Air.in :default @06:43:20.78 Air.hold @06:43:20.89 Lysis1.in :default @06:43:21.09 Lysis1.hold @06:43:21.40 Air.in :glide @06:43:21.49 Air.seek :fullVent @06:43:22.74 Handoff.select :waste @06:43:22.94 Recovered 764uL lysate @13.5psi @06:43:22.97 delay 3 seconds @06:43:26.08 After 3 delay Recovered 764uL lysate @-0.2psi @06:43:26.10 delay 5 seconds @06:43:31.18 Gate.power :heat,:OFF @06:43:31.21 Gate.power :cartridge,:OFF @06:43:31.37 PinPort.in :unlocking @06:43:31.47 PinPort.seek :clear @06:43:31.99 Archive.in :unlocking @06:43:32.18 Archive.seek :clear @06:43:32.32 Air.in :unlocking @06:43:32.52 Air.seek :clear @06:43:32.66 Lysis2.in :unlocking @06:43:32.76 Lysis2.seek :clear @06:43:37.53 Lysis1.in :unlocking @06:43:37.73 Lysis1.seek :clear @06:43:37.91 Plunge.in :unlocking @06:43:38.02 Plunge.seek :clear @06:59:38.27 -> cleanPortable @06:59:38.82 Waiting for nadia to answer "LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue" @06:59:40.64 Answered LOAD the cartridge blank, INTAKE line plumbed to MillQ water, push return to continue"" @06:59:40.78 Lysis1.in :fast @06:59:40.88 Lysis1.seek :locked @06:59:43.52 Plunge.in :default @06:59:43.63 Plunge.seek :locked @06:59:43.87 Air.in :fast @06:59:43.96 Air.seek :locked @06:59:45.00 Powering cartridge bus @06:59:47.70 Gate.power :cartridge,:OFF @06:59:48.30 Mounted archive Cartridge @06:59:48.38 Toroid.seek :bypassed @06:59:48.51 Priming sample loop w/10ml, bypass w/10ml @06:59:48.53 SamplePump.setPosition! 0ml @06:59:48.65 Bypass.open @06:59:50.32 Intake.open @06:59:52.19 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:59:54.82 Vacuum tare is 53% of full scale at 22C (drifted 1%) @06:59:55.10 Abs.in :default @06:59:55.19 Abs.hold @06:59:55.51 SamplePump.in :start @06:59:55.62 SamplePump.seek 10ml,1:10 @06:59:56.20 SamplePump.configure :prime @07:00:07.91 Abs.stop @07:00:08.00 SamplePump.setPosition! 0ml @07:00:08.10 Exhaust.open @07:00:09.75 Bypass.close @07:00:11.48 Abs.in :default @07:00:11.63 Abs.hold @07:00:12.05 SamplePump.in :start @07:00:12.16 SamplePump.seek 10ml,1:10 @07:00:12.73 SamplePump.configure :prime @07:00:24.44 Abs.stop @07:00:24.55 Waiting for nadia to answer "INTAKE line plumbed to 10% Bleach, push return to continue" @07:00:47.36 Answered INTAKE line plumbed to 10% Bleach, push return to continue"" @07:00:47.38 Toroid.seek :bypassed @07:00:47.53 Priming sample loop w/10ml, bypass w/10ml @07:00:47.57 SamplePump.setPosition! 0ml @07:00:47.69 Exhaust.close @07:00:48.53 Bypass.open @07:00:50.41 Abs.in :default @07:00:50.49 Abs.hold @07:00:50.82 SamplePump.in :start @07:00:50.93 SamplePump.seek 10ml,1:10 @07:00:51.51 SamplePump.configure :prime @07:01:03.27 Abs.stop @07:01:03.38 SamplePump.setPosition! 0ml @07:01:03.47 Exhaust.open @07:01:05.03 Bypass.close @07:01:06.75 Abs.in :default @07:01:06.85 Abs.hold @07:01:07.29 SamplePump.in :start @07:01:08.25 SamplePump.seek 10ml,1:10 @07:01:08.39 SamplePump.configure :prime @07:01:20.43 Abs.stop @07:01:20.55 Toroid.seek :cartridge @07:01:24.93 Sampling 25.0ml @07:01:24.97 SamplePump.setPosition! 0ml @07:01:25.32 Abs.in :default @07:01:25.43 Abs.hold @07:01:25.82 SamplePump.in :start @07:01:25.95 SamplePump.seek 25ml,2:10 @07:01:26.51 SamplePump.configure :sample @07:01:56.03 Abs.stop @07:01:56.13 Intake.close @07:01:56.25 Exhaust.close @07:01:56.39 Handoff.select :close @07:01:56.49 Toroid.seek :bypassed @07:02:01.00 Sampled 25.0ml @07:02:01.05 delay 10:00 @07:12:01.13 Waiting for nadia to answer "Move INTAKE line to MillQ water" @07:12:01.18 Answered Move INTAKE line to MillQ water"" @07:12:01.21 Toroid.seek :bypassed @07:12:01.39 Priming sample loop w/50ml, bypass w/25ml @07:12:01.44 SamplePump.setPosition! 0ml @07:12:01.56 Bypass.open @07:12:03.36 Intake.open @07:12:05.14 Abs.in :default @07:12:05.22 Abs.hold @07:12:05.55 SamplePump.in :start @07:12:05.66 SamplePump.seek 25ml,2:10 @07:12:06.24 SamplePump.configure :prime @07:12:35.72 Abs.stop @07:12:35.81 SamplePump.setPosition! 0ml @07:12:35.91 Exhaust.open @07:12:37.47 Bypass.close @07:12:39.24 Abs.in :default @07:12:39.34 Abs.hold @07:12:39.75 SamplePump.in :start @07:12:39.87 SamplePump.seek 50ml,3:50 @07:12:40.44 SamplePump.configure :prime @07:13:39.50 Abs.stop @07:13:39.61 Toroid.seek :cartridge @07:13:44.00 Sampling 25.0ml @07:13:44.02 SamplePump.setPosition! 0ml @07:13:45.24 Abs.in :default @07:13:45.35 Abs.hold @07:13:45.77 SamplePump.in :start @07:13:45.88 SamplePump.seek 25ml,2:35 @07:13:46.46 SamplePump.configure :sample @07:14:15.95 Abs.stop @07:14:16.04 Intake.close @07:14:16.11 Exhaust.close @07:14:16.31 Toroid.seek :bypassed @07:14:20.75 Sampled 25.0ml @07:14:21.15 Air.in :unlocking @07:14:21.38 Air.seek :clear @07:14:22.43 Lysis1.in :unlocking @07:14:22.56 Lysis1.seek :clear @07:14:22.77 Plunge.in :unlocking @07:14:22.88 Plunge.seek :clear @07:14:23.93 Portable sample Path clean, ready for next sample @07:30:41.73 -> ESP.shutdown @07:30:41.95 Gate.power :analytic3,:OFF @07:30:42.01 Gate.power :motor,:OFF @07:30:42.04 Gate.power :analytic1,:OFF @07:30:42.08 Gate.power :analytic2,:OFF @07:30:42.13 Gate.power :core,:OFF @07:30:42.16 Gate.power :main,:OFF [:analytic3,:motor,:analytic1,:analytic2,:core,:main] @07:30:44.88 -> exit @07:30:44.91 Session Ended