@08:23:14.46PST12-Dec-25 <MAIN> 
Environmental Sample Processor built 19-Nov-25 (c389e51a)
@08:23:17.83 >BREAK< ESP3 Gateway v1.44 for core bus
@08:23:17.87 >BREAK< ESP3 Gateway v1.44 for cartridge bus
@08:23:20.90 
Configuration for koa by nadia@mbari.org 11/18/25
/home/esp/esp2/type/auv/koa/configure.rb
@08:23:21.46 Sea pressure (Abs) tare is 6% of full scale
@08:23:29.89 Polling Can every 2:00
@08:23:30.35 Clients may connect on TCP port 9999
Connecting to 10.89.11.2:9999
@08:23:30.39 Waiting for espclients...
@08:23:30.80 <LRAUV> Session Begun
@08:23:32.74 -> showStatus
true
@08:23:34.75 -> Cmd.loadCartridge
@08:23:34.83 Gate.power :main,:ON,limit to 5000mA
@08:23:34.84 Gate.power :analytic1,:ON,limit to 4000mA
@08:23:34.87 Gate.power :analytic2,:ON,limit to 4000mA
@08:23:34.89 Gate.power :analytic3,:ON,limit to 4000mA
@08:23:34.94 Powering core bus
@08:23:37.65 Plunge.configure :default
@08:23:37.73 SamplePump.configure :default
@08:23:37.82 Toroid.configure :default
@08:23:37.90 Carousel.configure :default
@08:23:37.97 Abs.configure :default
@08:23:38.05 Archive.configure :default
@08:23:38.12 Air.configure :default
@08:23:38.18 Lysis1.configure :default
@08:23:38.24 Lysis2.configure :default
@08:23:38.33 PinPort.configure :default
@08:23:38.39 WARNING:  Multiple controller firmware versions in use
[:sampler0, :syringe1, :sampler1, :valve0, :ambient0, :valve1, :syringe0]=>4.93, [:carousel0]=>4.94
@08:23:38.41 Gate.power :motor,:ON,limit to 5000mA
@08:23:38.65 <Plunge_home> Plunge.home.jog 0
@08:23:39.16 <Lysis1_home> Lysis1.home.jog 0
@08:23:39.21 <Carousel_home> Carousel.home.jog 0
@08:23:39.24 <Lysis2_home> Lysis2.home.jog 0
@08:23:39.27 <Air_home> Air.home.jog 0
@08:23:39.31 <Archive_home> Archive.home.jog 0
@08:23:39.36 <Toroid_home> Toroid.home.jog 0
@08:23:39.42 <PinPort_home> PinPort.home.jog 0
@08:23:39.94 <Plunge_home> Plunge.in :homing
@08:23:40.24 Plunge.jog 1530
@08:23:40.28 <Lysis2_home> Lysis2.in :homing
@08:23:40.31 <Lysis1_home> Lysis1.in :homing
@08:23:40.34 <Air_home> Air.in :homing
@08:23:40.36 <Toroid_home> Toroid.in :homing
@08:23:40.41 <Archive_home> Archive.in :homing
@08:23:40.44 <PinPort_home> PinPort.in :homing
@08:23:40.65 <Lysis2_home> Lysis2.jog 305
@08:23:40.72 <PinPort_home> PinPort.jog -1203
@08:23:40.77 <Lysis1_home> Lysis1.jog 307
@08:23:40.81 <Air_home> Air.jog 362
@08:23:40.85 <Toroid_home> Toroid.jog 1304
@08:23:40.87 <Archive_home> Archive.jog 328
@08:23:41.31 <Lysis2_home> Lysis2.setRawPosition! 50
@08:23:41.37 Lysis2.seek :home
@08:23:41.50 <Lysis1_home> Lysis1.setRawPosition! 50
@08:23:41.56 Lysis1.seek :home
@08:23:41.67 <Archive_home> Archive.setRawPosition! 50
@08:23:41.69 <Air_home> Air.setRawPosition! 0
@08:23:41.76 <Lysis2_home> Lysis2.in :default
@08:23:41.81 <Archive_home> Archive.seek :home
@08:23:42.18 <Air_home> Air.seek :home
@08:23:42.44 Air.in :default
@08:23:42.46 <Plunge_home> Plunge.setRawPosition! 50
@08:23:42.50 <Lysis1_home> Lysis1.in :default
@08:23:42.64 <Plunge_home> Plunge.seek :home
@08:23:42.74 <PinPort_home> PinPort.setRawPosition! 0
@08:23:42.76 <Archive_home> Archive.in :default
@08:23:42.85 <PinPort_home> PinPort.seek :home
@08:23:42.98 PinPort.in :default
@08:23:42.99 <Plunge_home> Plunge.in :default
@08:23:45.33 <Toroid_home> Toroid.setRawPosition! 0
@08:23:45.39 Toroid.seek :home
@08:23:45.46 Toroid.in :default
@08:23:45.53 <clearToroid> Toroid.seek :clear
@08:23:45.67 <LRAUV> PinPort.in :unlocking
@08:23:45.73 PinPort.seek :clear
@08:23:49.14 Air.seek :clear
@08:23:50.03 Lysis1.seek :clear
@08:23:50.81 Lysis2.seek :clear
@08:23:51.60 Archive.seek :clear
@08:23:52.38 Carousel.in :default
@08:23:52.50 Carousel.home.seek 30
@08:23:52.76 Selecting Cartridge 29
@08:23:53.02 Carousel.seek 29
@08:23:53.55 Carousel.reconfigure :align
@08:23:53.66 Lysis1.in :fast
@08:23:53.72 Lysis1.seek :locked
@08:23:56.20 <Plunge> Plunge.seek :locked
@08:23:56.34 <Air> Air.in :fast
@08:23:56.40 Air.seek :locked
@08:23:57.31 <LRAUV> Carousel.reconfigure :locked
@08:23:57.45 Powering cartridge bus
@08:24:00.08 Gate.setIR 1750,5000,0,6
@08:24:00.21 Gate.setIR 0,5000,0,6
@08:24:00.29 PuckHeater.configure :default
@08:24:00.35 Gate.power :heat,:ON,limit to 5000mA
@08:24:00.54 Mounted lysis Cartridge board#J59974
@08:24:00.58 Using default cartridge heat loss curve for HeatingFunctionInCarousel
29
@08:24:14.83 -> Cmd.startFiltering
@08:24:15.08 <FILTERING> Preparing to filter lyseHoldBS_bac cartridge in slot 29
84.17
@08:24:15.30 <sealing> Air.in :default
@08:24:15.42 <FILTERING> Priming sample loop w/200ml, bypass w/10ml
@08:24:15.43 <sealing> Air.seek :sealed
@08:24:15.47 <FILTERING> SamplePump.setPosition! 0ml
@08:24:15.56 Bypass.open
@08:24:17.19 Intake.open
@08:24:18.31 Sea 49.0m deep (87.1psia)
@08:24:18.33 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@08:24:20.98 Vacuum tare is 52% of full scale at 13C (drifted 1%)
@08:24:21.10 <closeIntake> Intake.close
@08:24:21.17 <closeBypass> Bypass.close
@08:24:22.06 <FILTERING> Verifying toroid holds at least 0.6..0.85 of 86.9psia
@08:24:22.09 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi
@08:24:26.66 Bypass.open
@08:24:27.52 Intake.open
@08:24:29.26 Abs.in :default
@08:24:29.32 Abs.hold
@08:24:29.53 SamplePump.in :start
@08:24:29.59 SamplePump.seek 10ml,1:10
@08:24:30.22 <pumpStarted> SamplePump.configure :prime
@08:24:43.25 <FILTERING> Abs.stop
@08:24:43.30 SamplePump.setPosition! 0ml
@08:24:43.35 Exhaust.open
@08:24:44.97 <closeBypass> Bypass.close
@08:24:46.61 <FILTERING> Abs.in :default
@08:24:46.67 Abs.hold
@08:24:46.79 Sea 49.0m deep (87.1psia)
@08:24:46.99 SamplePump.in :start
@08:24:47.05 SamplePump.seek 200ml,13:50
@08:24:47.64 <pumpStarted> SamplePump.configure :prime
@08:29:12.66 <FILTERING> Abs.stop
@08:29:12.73 Bypass.open
@08:29:13.59 Intake.close
@08:29:14.71 Toroid.in :hold
@08:29:14.76 Toroid.seek :crack
@08:29:17.23 Toroid.seek :cartridge
@08:29:19.35 Toroid.in :default
@08:29:19.41 Toroid.seek :clear
@08:29:23.34 <closeExhaust> Exhaust.close
@08:29:23.41 <closeBypass> Bypass.close
@08:29:24.31 <FILTERING> Verifying cartridge holds at least 0.65..0.85 of 84.9psia
@08:29:24.34 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi
@08:29:28.90 Cartridge pressure fell 1% to 83.8psia
@08:29:28.98 Sampling 1000.0ml
@08:29:29.00 SamplePump.setPosition! 0ml
@08:29:29.06 Exhaust.open
@08:29:29.96 Intake.open
@08:29:31.17 Abs.in :default
@08:29:31.22 Abs.hold
@08:29:31.34 Sea 47.8m deep (85.3psia)
@08:29:31.51 SamplePump.in :start
@08:29:31.58 SamplePump.seek 1000ml,1:07:10
@08:29:32.20 <pumpStarted> SamplePump.configure :sample
@08:29:34.58 <sampling> Cartridge 29 filtering 2/1000ml at 0.69ml/s with 22.3psi vacuum 50.1m deep (88.7psia)
@08:30:34.63 Cartridge 29 filtering 36/1000ml at 0.57ml/s with 22.4psi vacuum 47.6m deep (85.0psia)
@08:31:34.63 Cartridge 29 filtering 70/1000ml at 0.56ml/s with 24.5psi vacuum 47.9m deep (85.4psia)
@08:32:34.64 Cartridge 29 filtering 103/1000ml at 0.55ml/s with 26.3psi vacuum 43.2m deep (78.5psia)
@08:33:34.65 Cartridge 29 filtering 136/1000ml at 0.55ml/s with 21.9psi vacuum 49.3m deep (87.5psia)
@08:34:34.63 Cartridge 29 filtering 168/1000ml at 0.54ml/s with 24.0psi vacuum 44.6m deep (80.6psia)
@08:35:34.63 Cartridge 29 filtering 200/1000ml at 0.54ml/s with 24.3psi vacuum 45.7m deep (82.2psia)
@08:36:34.63 Cartridge 29 filtering 232/1000ml at 0.53ml/s with 22.6psi vacuum 49.0m deep (87.1psia)
@08:37:34.63 Cartridge 29 filtering 264/1000ml at 0.53ml/s with 23.2psi vacuum 44.5m deep (80.5psia)
@08:38:34.63 Cartridge 29 filtering 295/1000ml at 0.52ml/s with 23.4psi vacuum 50.5m deep (89.3psia)
@08:39:34.63 Cartridge 29 filtering 325/1000ml at 0.51ml/s with 25.9psi vacuum 43.0m deep (78.3psia)
@08:40:34.63 Cartridge 29 filtering 356/1000ml at 0.50ml/s with 22.3psi vacuum 48.5m deep (86.4psia)
@08:41:34.63 Cartridge 29 filtering 386/1000ml at 0.50ml/s with 27.4psi vacuum 39.8m deep (73.5psia)
@08:42:34.63 Cartridge 29 filtering 415/1000ml at 0.49ml/s with 25.4psi vacuum 43.0m deep (78.3psia)
@08:43:34.63 Cartridge 29 filtering 444/1000ml at 0.48ml/s with 26.8psi vacuum 44.7m deep (80.7psia)
@08:44:34.63 Cartridge 29 filtering 472/1000ml at 0.48ml/s with 24.9psi vacuum 47.5m deep (85.0psia)
@08:45:34.63 Cartridge 29 filtering 500/1000ml at 0.47ml/s with 18.0psi vacuum 56.1m deep (97.6psia)
@08:46:34.63 Cartridge 29 filtering 528/1000ml at 0.46ml/s with 17.8psi vacuum 53.2m deep (93.4psia)
@08:47:34.63 Cartridge 29 filtering 555/1000ml at 0.45ml/s with 21.5psi vacuum 50.5m deep (89.4psia)
@08:48:34.63 Cartridge 29 filtering 582/1000ml at 0.45ml/s with 24.8psi vacuum 47.2m deep (84.5psia)
@08:49:34.63 Cartridge 29 filtering 608/1000ml at 0.44ml/s with 26.5psi vacuum 45.6m deep (82.1psia)
@08:50:35.00 Cartridge 29 filtering 634/1000ml at 0.43ml/s with 25.7psi vacuum 44.1m deep (79.9psia)
@08:51:34.63 Cartridge 29 filtering 660/1000ml at 0.42ml/s with 22.1psi vacuum 47.0m deep (84.2psia)
@08:52:34.63 Cartridge 29 filtering 685/1000ml at 0.42ml/s with 21.8psi vacuum 49.5m deep (87.9psia)
@08:53:34.63 Cartridge 29 filtering 709/1000ml at 0.41ml/s with 20.5psi vacuum 53.9m deep (94.3psia)
@08:54:34.63 Cartridge 29 filtering 733/1000ml at 0.40ml/s with 21.8psi vacuum 52.1m deep (91.6psia)
@08:55:34.63 Cartridge 29 filtering 757/1000ml at 0.39ml/s with 25.7psi vacuum 47.7m deep (85.1psia)
@08:56:34.63 Cartridge 29 filtering 780/1000ml at 0.39ml/s with 26.4psi vacuum 45.7m deep (82.2psia)
@08:57:34.63 Cartridge 29 filtering 803/1000ml at 0.38ml/s with 19.1psi vacuum 52.5m deep (92.3psia)
@08:58:34.63 Cartridge 29 filtering 825/1000ml at 0.37ml/s with 26.5psi vacuum 45.0m deep (81.3psia)
@08:59:34.63 Cartridge 29 filtering 847/1000ml at 0.37ml/s with 25.1psi vacuum 47.5m deep (84.8psia)
@09:00:34.63 Cartridge 29 filtering 869/1000ml at 0.36ml/s with 25.1psi vacuum 43.8m deep (79.4psia)
@09:01:34.63 Cartridge 29 filtering 890/1000ml at 0.35ml/s with 30.1psi vacuum 38.8m deep (72.1psia)
@09:02:34.63 Cartridge 29 filtering 911/1000ml at 0.35ml/s with 28.4psi vacuum 40.3m deep (74.2psia)
@09:03:34.63 Cartridge 29 filtering 931/1000ml at 0.34ml/s with 19.6psi vacuum 49.8m deep (88.4psia)
@09:04:34.63 Cartridge 29 filtering 952/1000ml at 0.34ml/s with 18.5psi vacuum 56.5m deep (98.2psia)
@09:05:34.63 Cartridge 29 filtering 972/1000ml at 0.33ml/s with 21.7psi vacuum 51.1m deep (90.2psia)
@09:06:34.63 Cartridge 29 filtering 991/1000ml at 0.33ml/s with 23.8psi vacuum 46.3m deep (83.1psia)
@09:07:01.58 <FILTERING> Abs.stop
@09:07:01.65 <closeIntake> Intake.close
@09:07:01.73 <closeExhaust> Exhaust.close
@09:07:01.91 <FILTERING> Toroid.in :hold
@09:07:01.98 Toroid.seek :bypass
@09:07:05.57 Toroid.in :default
@09:07:05.63 Toroid.seek :clear
@09:07:09.21 Sampled  1000.0ml
@09:07:10.93 <LRAUV> -> Cmd.startProcessing
60.0
@09:07:11.26 <PROCESSING> Handoff.select :waste
@09:07:11.40 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@09:07:17.99 Syringe tare is 53% of full scale at 14C (drifted 2%)
@09:07:18.02 PinPort.in :locking
@09:07:18.08 PinPort.seek :locked
@09:07:20.53 <posArchive> Archive.in :fast
@09:07:20.59 <posLyS2> Lysis2.in :fast
@09:07:20.63 <PROCESSING> Lysis1.seek 1250uL
@09:07:20.74 <posArchive> Archive.seek :empty
@09:07:21.21 <posLyS2> Lysis2.seek :empty
@09:07:29.60 <PROCESSING> Evacuating puck with filter bubble point of 50psi
@09:07:29.63 PinPort.seek :locked
@09:07:29.71 Handoff.select :waste
@09:07:29.74 Plunge.seek :puckBottom
@09:07:31.05 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@09:07:37.62 Air syringe pumping up to 1 strokes to reach 8psi
@09:07:37.63 Air stroke #1 of 1
@09:07:37.73 Air.in :pressurize
@09:07:37.78 Air.seek :pressurized
@09:07:42.85 0.1psi after pressurize
@09:07:42.87 Air.in :compress
@09:07:42.93 Air.seek :evac
@09:07:49.96 1.0psi after compress
@09:08:00.53 -0.0psi after compress (stable)
@09:08:00.55 Air.in :default
@09:08:00.66 Air.hold
@09:08:00.83 at 0.1psi Opened to waste
@09:08:00.84 Handoff.select :blocked
@09:08:00.98 Plunge.seek :puckTop
@09:08:01.88 Air syringe pumping up to 8 strokes to reach 50psi
@09:08:01.89 Air stroke #1 of 8
@09:08:01.99 Air.in :compress
@09:08:02.05 Air.seek :evac
@09:08:02.17 10.4psi after compress
@09:08:12.74 10.8psi after compress (stable)
@09:08:12.76 Air.in :gulp
@09:08:12.83 Air.seek :topVent
@09:08:19.42 10.7psi after gulp
@09:08:24.99 10.8psi after gulp (stable)
@09:08:25.00 Air stroke #2 of 8
@09:08:25.09 Air.in :pressurize
@09:08:25.15 Air.seek :pressurized
@09:08:31.58 10.7psi after pressurize
@09:08:31.60 Air.in :compress
@09:08:31.67 Air.seek :evac
@09:08:38.69 24.3psi after compress
@09:08:53.77 28.8psi after compress (stable)
@09:08:53.79 Air.in :gulp
@09:08:53.86 Air.seek :topVent
@09:09:00.44 28.9psi after gulp
@09:09:06.01 28.7psi after gulp (stable)
@09:09:06.02 Air stroke #3 of 8
@09:09:06.12 Air.in :pressurize
@09:09:06.17 Air.seek :pressurized
@09:09:12.60 28.6psi after pressurize
@09:09:12.62 Air.in :compress
@09:09:12.68 Air.seek :evac
@09:09:19.70 38.9psi after compress
@09:09:32.77 43.9psi after compress (stable)
@09:09:32.79 Air.in :gulp
@09:09:32.86 Air.seek :topVent
@09:09:39.44 44.6psi after gulp
@09:09:45.01 44.2psi after gulp (stable)
@09:09:45.02 Air stroke #4 of 8
@09:09:45.12 Air.in :pressurize
@09:09:45.17 Air.seek :pressurized
@09:09:51.60 44.3psi after pressurize
@09:09:51.62 Air.in :compress
@09:09:51.68 Air.seek :evac
@09:09:58.55 Reached 50.8 of 50.0psi after pumping 7095uL (3.99 strokes)
@09:09:58.57 Air.in :default
@09:09:58.63 Air.hold
@09:09:58.71 Plunge.seek :puckBottom
@09:09:59.60 delay 3 seconds
@09:10:02.61 Handoff.select :waste
@09:10:02.74 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@09:10:15.31 delay 1:00
@09:11:15.34 Handoff.select :blocked
@09:11:15.47 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@09:11:38.04 Handoff.select :waste
@09:11:38.16 0.8psi at evacEnd
@09:11:38.17 Handoff.select :blocked
@09:11:38.31 Plunge.seek :puckTop
@09:11:39.20 Air.in :fast
@09:11:39.26 Air.seek :topVent
@09:11:45.84 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi
@09:11:52.41 Stable @12.0psi after Handoff blocked
@09:11:52.43 Air syringe pumping up to 8 strokes to reach 35psi
@09:11:52.45 Air stroke #1 of 8
@09:11:52.55 Air.in :pressurize
@09:11:52.97 Air.seek :pressurized
@09:11:59.40 11.8psi after pressurize
@09:11:59.42 Air.in :compress
@09:11:59.49 Air.seek :evac
@09:12:06.51 21.5psi after compress
@09:12:18.59 25.2psi after compress (stable)
@09:12:18.61 Air.in :gulp
@09:12:18.67 Air.seek :topVent
@09:12:25.25 25.3psi after gulp
@09:12:30.82 25.0psi after gulp (stable)
@09:12:30.83 Air stroke #2 of 8
@09:12:30.93 Air.in :pressurize
@09:12:30.98 Air.seek :pressurized
@09:12:37.41 25.0psi after pressurize
@09:12:37.43 Air.in :compress
@09:12:37.50 Air.seek :evac
@09:12:44.52 33.2psi after compress
@09:12:44.73 Reached 34.2 of 35.0psi after pumping 3553uL (2.00 strokes)
@09:12:44.75 Air.in :default
@09:12:44.82 Air.hold
@09:12:44.89 Plunge.seek :puckBottom
@09:12:45.79 delay 3 seconds
@09:12:48.80 Handoff.select :waste
@09:12:48.93 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@09:12:55.50 delay 1:00
@09:13:55.52 Handoff.select :blocked
@09:13:55.65 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@09:14:18.26 Handoff.select :waste
@09:14:18.38 1.3psi at evacEnd
@09:14:18.40 Plunge.seek :pTop
@09:14:19.30 delay 5 seconds
@09:14:24.33 Air.in :glide
@09:14:24.37 Air.seek :fullVent
@09:14:26.19 delay 2 seconds
@09:14:28.22 Plunge.seek :pBottom
@09:14:29.10 Seawater evacuated, Pressure above/below filter released.
@09:14:29.12 Plunge.seek :locked
@09:14:30.42 Handoff.select :waste
@09:14:30.52 Lysis1.in :slow
@09:14:30.58 Lysis1.seek 950uL
@09:14:44.73 delay 5 seconds
@09:14:49.75 Handoff.select :blocked
@09:14:49.89 Plunge.seek :puckTop
@09:14:50.81 Lysis1.seek 350uL
@09:15:18.97 delay 3 seconds
@09:15:22.05 Air.in mix with 2.32937 parts Lysis1
@09:15:22.49 <Airmixer> Air.seek :evac
@09:15:22.53 <PROCESSING> Lysis1.seek 190uL
@09:15:30.35 Air.in :fast
@09:15:30.41 Air.seek :full
@09:15:37.05 Lysis1.seek :empty
@09:15:46.18 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @18.9psi
@09:15:46.20 Plunge.seek :puckBottom
@09:15:47.18 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @15.3psi
@09:15:47.20 Polling Can every 2:00
@09:15:47.74 PuckHeater.configure :default #ambient 14.2C
@09:15:47.80 PuckHeater.seek 71.6C
@09:17:21.03 PuckHeater.hold 2:00,71.6C
@09:19:20.92 Polling Can every 2:00
@09:19:21.45 PuckHeater.configure :default #ambient 14.2C
@09:19:21.51 PuckHeater.seek 70.5C
@09:19:21.66 PuckHeater.hold 2:00,70.5C
@09:21:21.65 Polling Can every 2:00
@09:21:22.19 PuckHeater.configure :default #ambient 14.2C
@09:21:22.25 PuckHeater.hold 18:00,69.5C
@09:39:20.47 Polling Can every 2:00
@09:39:21.00 PuckHeater.configure :default #ambient 14.2C
@09:39:21.06 PuckHeater.seek 105.3C
@09:40:29.93 PuckHeater.hold 2:00,105.3C
@09:42:29.82 Polling Can every 2:00
@09:42:30.36 PuckHeater.configure :default #ambient 14.3C
@09:42:30.42 PuckHeater.seek 104.3C
@09:42:30.55 PuckHeater.hold 2:00,104.3C
@09:44:30.46 Polling Can every 2:00
@09:44:30.99 PuckHeater.configure :default #ambient 14.2C
@09:44:31.05 PuckHeater.hold 2:00,103.2C
@09:46:31.29 delay 5:00
@09:51:31.35 Lysate cooked @7.9psi
@09:51:31.37 Plunge.seek :puckTop
@09:51:32.27 Air syringe pumping up to 5 strokes to reach 40psi
@09:51:32.28 Air stroke #1 of 5
@09:51:32.37 Air.in :pressurize
@09:51:32.43 Air.seek :pressurized
@09:51:38.85 7.5psi after pressurize
@09:51:38.87 Air.in :compress
@09:51:38.95 Air.seek :evac
@09:51:44.57 Reached 40.9 of 40.0psi after pumping 1697uL (0.96 strokes)
@09:51:44.59 Air.in :default
@09:51:44.65 Air.hold
@09:51:44.73 delay 2 seconds
@09:51:46.75 Air.in :fast
@09:51:46.80 Air.seek :full
@09:51:53.16 Plunge.seek :puckBottom
@09:51:54.11 Lysis1.seek 138uL
@09:52:00.71 delay 2 seconds
@09:52:02.73 Plunge.seek :puckTop
@09:52:03.62 Lysis1.seek :empty
@09:52:10.22 Plunge.seek :puckBottom
@09:52:11.28 Lysis1.in :extract
@09:52:11.37 Lysis1.seek 675uL,nil
@09:52:13.72 <pump> Air.in :pressurize
@09:52:13.78 Air.seek :pressurized
@09:52:20.26 12.8psi after pressurize
@09:52:20.28 Air.in :compressLysate
@09:52:20.35 Air.seek :evac
@09:52:43.09 Reached 15.8 of 15.5psi after pumping 1706uL (0.96 strokes)
@09:52:43.11 Air.in :default
@09:52:43.19 Air.hold
@09:52:44.01 Air.in :compressLysate
@09:52:44.07 Air.seek :evac
@09:52:49.22 12.9psi after compress
@09:52:59.83 13.7psi after compress (stable)
@09:53:05.02 Air.in :gulp
@09:53:05.07 Air.seek :topVent
@09:53:11.68 14.3psi after gulp
@09:53:12.28 Air.in :default
@09:53:12.34 Air.hold
@09:53:12.51 <PROCESSING> Pulled 675uL lysate @13.7psi
@09:53:12.68 Lysis1.seek 800uL,nil
@09:53:12.85 <pump> Air.in :pressurize
@09:53:12.92 Air.seek :pressurized
@09:53:19.36 13.0psi after pressurize
@09:53:19.38 Air.in :compressLysate
@09:53:19.45 Air.seek :evac
@09:53:47.50 14.3psi after compress
@09:53:59.13 14.3psi after compress (stable)
@09:54:02.94 Reached 15.2 of 15.5psi after pumping 1777uL (1.00 strokes)
@09:54:02.97 Air.in :default
@09:54:03.04 Air.hold
@09:54:03.28 Air.in :compressLysate
@09:54:03.35 Air.seek :evac
@09:54:03.58 15.0psi after compress
@09:54:16.26 Reached 15.2 of 15.5psi after pumping 1777uL (1.00 strokes)
@09:54:16.28 Air.in :default
@09:54:16.36 Air.hold
@09:54:16.66 Air.in :compressLysate
@09:54:16.73 Air.seek :evac
@09:54:16.95 14.9psi after compress
@09:54:18.71 Air.in :default
@09:54:18.78 Air.hold
@09:54:19.35 <PROCESSING> Air.in :glide
@09:54:19.42 Air.seek :fullVent
@09:54:21.22 Handoff.select :waste
@09:54:21.35 Recovered 802uL lysate @13.5psi
@09:54:21.37 delay 3 seconds
@09:54:24.44 After 3 delay Recovered 802uL lysate @-0.2psi
@09:54:25.65 <LRAUV> -> Cmd.stop
@09:54:25.75 <closeHandoff> Handoff.select :close
@09:54:25.89 <LRAUV> Gate.power :heat,:OFF
@09:54:25.91 Gate.power :cartridge,:OFF
@09:54:26.00 PinPort.in :unlocking
@09:54:26.06 PinPort.seek :clear
@09:54:28.49 Carousel.in :default
@09:54:28.60 Carousel.home.seek 29
@09:54:28.92 Plunge.seek :locked
@09:54:30.28 <Archive> Archive.in :unlocking
@09:54:30.42 Archive.seek :clear
@09:54:30.46 <Air> Air.in :unlocking
@09:54:30.61 Air.seek :clear
@09:54:30.72 <Lysis1> Lysis1.in :unlocking
@09:54:30.80 Lysis1.seek :clear
@09:54:30.93 <Lysis2> Lysis2.in :unlocking
@09:54:30.99 Lysis2.seek :clear
@09:54:35.75 <LRAUV> Gate.power :motor,:OFF
@09:54:35.77 Gate.power :analytic1,:OFF
@09:54:35.80 Gate.power :analytic2,:OFF
@09:54:35.82 Gate.power :analytic3,:OFF
@09:54:35.85 Gate.power :core,:OFF
@09:54:35.87 Gate.power :main,:OFF
@09:54:35.89 Safely stopped and ready to power off
:STOPPED