@08:22:09.99PST04-Dec-25 <MAIN> 
Environmental Sample Processor built 19-Nov-25 (c389e51a)
@08:22:13.51 >BREAK< ESP3 Gateway v1.44 for core bus
@08:22:13.53 >BREAK< ESP3 Gateway v1.44 for cartridge bus
@08:22:16.65 
Configuration for koa by nadia@mbari.org 11/18/25
/home/esp/esp2/type/auv/koa/configure.rb
@08:22:17.21 Sea pressure (Abs) tare is 6% of full scale
@08:22:25.13 Polling Can every 2:00
@08:22:25.61 Clients may connect on TCP port 9999
@08:22:25.62 Connecting to 10.89.11.2:9999
@08:22:25.67 Waiting for espclients...
@08:22:26.00 <LRAUV> Session Begun
@08:22:27.94 -> showStatus
true
@08:22:29.95 -> Cmd.loadCartridge -15
@08:22:30.04 Gate.power :main,:ON,limit to 5000mA
@08:22:30.06 Gate.power :analytic1,:ON,limit to 4000mA
@08:22:30.09 Gate.power :analytic2,:ON,limit to 4000mA
@08:22:30.12 Gate.power :analytic3,:ON,limit to 4000mA
@08:22:30.16 Powering core bus
@08:22:32.87 Plunge.configure :default
@08:22:32.95 SamplePump.configure :default
@08:22:33.04 Toroid.configure :default
@08:22:33.12 Carousel.configure :default
@08:22:33.20 Abs.configure :default
@08:22:33.27 Archive.configure :default
@08:22:33.35 Air.configure :default
@08:22:33.41 Lysis1.configure :default
@08:22:33.48 Lysis2.configure :default
@08:22:33.56 PinPort.configure :default
@08:22:33.62 WARNING:  Multiple controller firmware versions in use
[:sampler0, :syringe1, :sampler1, :valve0, :ambient0, :valve1, :syringe0]=>4.93, [:carousel0]=>4.94
@08:22:33.65 Gate.power :motor,:ON,limit to 5000mA
@08:22:33.89 <Plunge_home> Plunge.home.jog 0
@08:22:34.39 <PinPort_home> PinPort.home.jog 0
@08:22:34.43 <Lysis2_home> Lysis2.home.jog 0
@08:22:34.46 <Air_home> Air.home.jog 0
@08:22:34.50 <Archive_home> Archive.home.jog 0
@08:22:34.52 <Carousel_home> Carousel.home.jog 0
@08:22:34.55 <Toroid_home> Toroid.home.jog 0
@08:22:34.62 <Lysis1_home> Lysis1.home.jog 0
@08:22:35.23 <Plunge_home> Plunge.in :homing
@08:22:35.40 Plunge.jog 1527
@08:22:35.46 <Lysis2_home> Lysis2.in :homing
@08:22:35.48 <Lysis1_home> Lysis1.in :homing
@08:22:35.51 <Air_home> Air.in :homing
@08:22:35.55 <Toroid_home> Toroid.in :homing
@08:22:35.58 <Archive_home> Archive.in :homing
@08:22:35.61 <PinPort_home> PinPort.in :homing
@08:22:35.87 <Lysis2_home> Lysis2.jog 1612
@08:22:35.92 <Lysis1_home> Lysis1.jog 306
@08:22:35.94 <Air_home> Air.jog 362
@08:22:35.97 <Archive_home> Archive.jog 324
@08:22:36.01 <Toroid_home> Toroid.jog 1300
@08:22:36.07 <PinPort_home> PinPort.jog -1203
@08:22:36.67 <Lysis1_home> Lysis1.setRawPosition! 50
@08:22:36.73 Lysis1.seek :home
@08:22:36.83 <Air_home> Air.setRawPosition! 0
@08:22:36.85 <Archive_home> Archive.setRawPosition! 50
@08:22:36.94 <Air_home> Air.seek :home
@08:22:36.98 <Archive_home> Archive.seek :home
@08:22:37.15 <Air_home> Air.in :default
@08:22:37.17 <Plunge_home> Plunge.setRawPosition! 50
@08:22:37.20 <Lysis1_home> Lysis1.in :default
@08:22:37.35 <Plunge_home> Plunge.seek :home
@08:22:37.45 <Lysis2_home> Lysis2.setRawPosition! 50
@08:22:37.47 <Archive_home> Archive.in :default
@08:22:37.57 <Lysis2_home> Lysis2.seek :home
@08:22:37.69 <Plunge_home> Plunge.in :default
@08:22:37.87 <Lysis2_home> Lysis2.in :default
@08:22:37.95 <PinPort_home> PinPort.setRawPosition! 0
@08:22:38.01 PinPort.seek :home
@08:22:38.10 PinPort.in :default
@08:22:40.53 <Toroid_home> Toroid.setRawPosition! 0
@08:22:40.58 Toroid.seek :home
@08:22:40.66 Toroid.in :default
@08:22:40.73 <clearToroid> Toroid.seek :clear
@08:22:40.86 <LRAUV> PinPort.in :unlocking
@08:22:40.94 PinPort.seek :clear
@08:22:44.39 Air.seek :clear
@08:22:45.28 Lysis1.seek :clear
@08:22:46.07 Lysis2.seek :clear
@08:22:46.92 Archive.seek :clear
@08:22:47.71 Carousel.in :default
@08:22:47.83 Carousel.home.seek 53
@08:22:48.09 Selecting Cartridge 52
@08:22:48.36 Carousel.seek 52
@08:22:48.90 Carousel.reconfigure :align
@08:22:49.01 Lysis1.in :fast
@08:22:49.07 Lysis1.seek :locked
@08:22:51.55 <Plunge> Plunge.seek :locked
@08:22:51.70 <Air> Air.in :fast
@08:22:51.75 Air.seek :locked
@08:22:52.65 <LRAUV> Carousel.reconfigure :locked
@08:22:52.79 Powering cartridge bus
@08:22:55.43 Gate.setIR 1750,5000,0,6
@08:22:55.55 Gate.setIR 0,5000,0,6
@08:22:55.63 PuckHeater.configure :default
@08:22:55.69 Gate.power :heat,:ON,limit to 5000mA
@08:22:55.90 Mounted lysis Cartridge board#J59945
@08:22:55.94 Using default cartridge heat loss curve for HeatingFunctionInCarousel
52
@08:23:10.00 -> Cmd.startFiltering
@08:23:10.25 <FILTERING> Preparing to filter lyseHoldBS_bac cartridge in slot 52
84.17
@08:23:10.47 <sealing> Air.in :default
@08:23:10.60 <FILTERING> Priming sample loop w/200ml, bypass w/10ml
@08:23:10.61 <sealing> Air.seek :sealed
@08:23:10.65 <FILTERING> SamplePump.setPosition! 0ml
@08:23:10.81 Bypass.open
@08:23:12.40 Intake.open
@08:23:13.49 Sea 5.2m deep (22.4psia)
@08:23:13.51 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@08:23:16.11 Vacuum tare is 52% of full scale at 13C (drifted 1%)
@08:23:16.24 Abs.in :default
@08:23:16.29 Abs.hold
@08:23:16.49 SamplePump.in :start
@08:23:16.56 SamplePump.seek 10ml,1:10
@08:23:17.19 <pumpStarted> SamplePump.configure :prime
@08:23:30.58 <FILTERING> Abs.stop
@08:23:30.65 SamplePump.setPosition! 0ml
@08:23:30.70 Exhaust.open
@08:23:32.27 <closeBypass> Bypass.close
@08:23:33.90 <FILTERING> Abs.in :default
@08:23:33.95 Abs.hold
@08:23:34.07 Sea 5.2m deep (22.4psia)
@08:23:34.23 SamplePump.in :start
@08:23:34.29 SamplePump.seek 200ml,13:50
@08:23:34.92 <pumpStarted> SamplePump.configure :prime
@08:27:59.90 <FILTERING> Abs.stop
@08:27:59.98 Bypass.open
@08:28:00.80 Intake.close
@08:28:01.77 Toroid.in :hold
@08:28:01.83 Toroid.seek :crack
@08:28:04.30 Toroid.seek :cartridge
@08:28:06.42 Toroid.in :default
@08:28:06.48 Toroid.seek :clear
@08:28:10.41 Pressurizing cartridge to 26psi above 23.0psia
@08:28:10.43 SamplePump.setPosition! 0ml
@08:28:10.48 Intake.open
@08:28:11.42 Bypass.close
@08:28:12.22 Exhaust.close
@08:28:13.04 Abs.in :default
@08:28:13.10 Abs.hold
@08:28:13.29 SamplePump.in :charge
@08:28:13.35 SamplePump.seek 2.3ml,40 seconds
@08:28:23.67 Abs.stop
@08:28:23.74 Intake.close
@08:28:24.70 Pressurized cartridge with 0.437ml to 26.6psi
@08:28:24.71 Isolated @44.7psia (21.7psi)
@08:28:24.74 Verifying cartridge holds at least 0.65..0.85 of 44.7psia
@08:28:24.77 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@08:28:29.35 Cartridge pressure fell 1% to 44.2psia
@08:28:29.44 Sampling 1000.0ml
@08:28:29.45 SamplePump.setPosition! 0ml
@08:28:29.52 Exhaust.open
@08:28:30.38 Intake.open
@08:28:31.81 Abs.in :default
@08:28:31.87 Abs.hold
@08:28:32.00 Sea 5.2m deep (22.3psia)
@08:28:32.17 SamplePump.in :start
@08:28:32.24 SamplePump.seek 1000ml,1:07:10
@08:28:32.86 <pumpStarted> SamplePump.configure :sample
@08:28:35.25 <sampling> Cartridge 52 filtering 2/1000ml at 0.53ml/s with 29.1psi vacuum
@08:29:35.29 Cartridge 52 filtering 35/1000ml at 0.56ml/s with 21.7psi vacuum 5.0m deep (22.0psia)
@08:30:35.29 Cartridge 52 filtering 68/1000ml at 0.55ml/s with 26.1psi vacuum
@08:31:35.29 Cartridge 52 filtering 101/1000ml at 0.54ml/s with 18.8psi vacuum 9.9m deep (29.3psia)
@08:32:35.29 Cartridge 52 filtering 133/1000ml at 0.53ml/s with 22.6psi vacuum 5.6m deep (23.0psia)
@08:33:35.29 Cartridge 52 filtering 164/1000ml at 0.52ml/s with 23.9psi vacuum 3.9m deep (20.5psia)
@08:34:35.29 Cartridge 52 filtering 195/1000ml at 0.51ml/s with 22.4psi vacuum 5.8m deep (23.2psia)
@08:35:35.34 Cartridge 52 filtering 225/1000ml at 0.51ml/s with 28.1psi vacuum
@08:36:35.29 Cartridge 52 filtering 255/1000ml at 0.50ml/s with 23.2psi vacuum 5.1m deep (22.2psia)
@08:37:35.29 Cartridge 52 filtering 285/1000ml at 0.49ml/s with 17.7psi vacuum 15.3m deep (37.3psia)
@08:38:35.29 Cartridge 52 filtering 314/1000ml at 0.48ml/s with 25.4psi vacuum 3.9m deep (20.5psia)
@08:39:35.29 Cartridge 52 filtering 343/1000ml at 0.48ml/s with 23.7psi vacuum 4.8m deep (21.8psia)
@08:40:35.33 Cartridge 52 filtering 371/1000ml at 0.47ml/s with 21.2psi vacuum 6.6m deep (24.5psia)
@08:41:35.29 Cartridge 52 filtering 399/1000ml at 0.46ml/s with 24.3psi vacuum
@08:42:35.29 Cartridge 52 filtering 426/1000ml at 0.45ml/s with 23.7psi vacuum 5.5m deep (22.8psia)
@08:43:35.29 Cartridge 52 filtering 452/1000ml at 0.45ml/s with 26.2psi vacuum
@08:44:35.29 Cartridge 52 filtering 479/1000ml at 0.44ml/s with 24.8psi vacuum 4.2m deep (20.9psia)
@08:45:35.29 Cartridge 52 filtering 504/1000ml at 0.43ml/s with 21.0psi vacuum 6.1m deep (23.6psia)
@08:46:35.29 Cartridge 52 filtering 530/1000ml at 0.42ml/s with 24.6psi vacuum 6.5m deep (24.3psia)
@08:47:35.29 Cartridge 52 filtering 554/1000ml at 0.41ml/s with 24.9psi vacuum
@08:48:35.29 Cartridge 52 filtering 579/1000ml at 0.40ml/s with 19.7psi vacuum 5.6m deep (23.0psia)
@08:49:35.29 Cartridge 52 filtering 602/1000ml at 0.40ml/s with 24.0psi vacuum
@08:50:35.29 Cartridge 52 filtering 626/1000ml at 0.39ml/s with 20.6psi vacuum 8.9m deep (27.9psia)
@08:51:35.29 Cartridge 52 filtering 648/1000ml at 0.38ml/s with 23.2psi vacuum
@08:52:35.29 Cartridge 52 filtering 671/1000ml at 0.37ml/s with 27.2psi vacuum
@08:53:35.29 Cartridge 52 filtering 693/1000ml at 0.36ml/s with 23.0psi vacuum 3.9m deep (20.4psia)
@08:54:35.29 Cartridge 52 filtering 714/1000ml at 0.36ml/s with 24.0psi vacuum 6.1m deep (23.6psia)
@08:55:35.29 Cartridge 52 filtering 735/1000ml at 0.35ml/s with 24.4psi vacuum 6.8m deep (24.7psia)
@08:56:35.29 Cartridge 52 filtering 755/1000ml at 0.34ml/s with 26.1psi vacuum
@08:57:35.29 Cartridge 52 filtering 775/1000ml at 0.33ml/s with 21.6psi vacuum 6.2m deep (23.8psia)
@08:58:35.29 Cartridge 52 filtering 795/1000ml at 0.33ml/s with 22.5psi vacuum
@08:59:35.29 Cartridge 52 filtering 814/1000ml at 0.32ml/s with 26.7psi vacuum 4.9m deep (22.0psia)
@09:00:35.60 Cartridge 52 filtering 833/1000ml at 0.31ml/s with 20.7psi vacuum 8.8m deep (27.6psia)
@09:01:35.29 Cartridge 52 filtering 851/1000ml at 0.31ml/s with 24.9psi vacuum
@09:02:35.29 Cartridge 52 filtering 869/1000ml at 0.30ml/s with 19.0psi vacuum 7.8m deep (26.2psia)
@09:03:35.29 Cartridge 52 filtering 887/1000ml at 0.29ml/s with 23.2psi vacuum 7.8m deep (26.2psia)
@09:04:35.29 Cartridge 52 filtering 904/1000ml at 0.28ml/s with 22.7psi vacuum 8.8m deep (27.7psia)
@09:05:35.29 Cartridge 52 filtering 920/1000ml at 0.28ml/s with 21.2psi vacuum 6.6m deep (24.5psia)
@09:06:35.29 Cartridge 52 filtering 937/1000ml at 0.27ml/s with 21.6psi vacuum 9.3m deep (28.4psia)
@09:07:35.34 Cartridge 52 filtering 953/1000ml at 0.26ml/s with 20.8psi vacuum 5.9m deep (23.4psia)
@09:08:35.29 Cartridge 52 filtering 968/1000ml at 0.26ml/s with 24.3psi vacuum 7.1m deep (25.2psia)
@09:09:35.29 Cartridge 52 filtering 983/1000ml at 0.25ml/s with 27.2psi vacuum
@09:10:35.29 Cartridge 52 filtering 998/1000ml at 0.25ml/s with 22.4psi vacuum 4.6m deep (21.5psia)
@09:10:35.44 <FILTERING> SamplePump.stop
@09:10:35.50 <closeIntake> Intake.close
@09:10:35.54 <closeExhaust> Exhaust.close
@09:10:35.76 <FILTERING> Toroid.in :hold
@09:10:35.83 Toroid.seek :bypass
@09:10:39.43 Toroid.in :default
@09:10:39.49 Toroid.seek :clear
@09:10:43.08 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged
@09:10:43.10 Abs.stop
@09:10:43.25 Sampled  997.9ml
@09:10:45.16 <LRAUV> -> Cmd.startProcessing
60.0
@09:10:45.51 <PROCESSING> Handoff.select :waste
@09:10:45.64 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@09:10:52.23 Syringe tare is 50% of full scale at 15C (drifted -1%)
@09:10:52.25 PinPort.in :locking
@09:10:52.33 PinPort.seek :locked
@09:10:54.77 <posArchive> Archive.in :fast
@09:10:54.82 <posLyS2> Lysis2.in :fast
@09:10:54.89 <PROCESSING> Lysis1.seek 1250uL
@09:10:54.95 <posArchive> Archive.seek :empty
@09:10:55.04 <posLyS2> Lysis2.seek :empty
@09:11:03.45 <PROCESSING> Evacuating puck with filter bubble point of 50psi
@09:11:03.48 PinPort.seek :locked
@09:11:03.57 Handoff.select :waste
@09:11:03.59 Plunge.seek :puckBottom
@09:11:05.28 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@09:11:11.86 Air syringe pumping up to 1 strokes to reach 8psi
@09:11:11.87 Air stroke #1 of 1
@09:11:11.97 Air.in :pressurize
@09:11:12.02 Air.seek :pressurized
@09:11:17.11 -0.1psi after pressurize
@09:11:17.13 Air.in :compress
@09:11:17.19 Air.seek :evac
@09:11:24.22 0.9psi after compress
@09:11:34.79 0.0psi after compress (stable)
@09:11:34.81 Air.in :default
@09:11:34.88 Air.hold
@09:11:35.06 at 0.1psi Opened to waste
@09:11:35.07 Handoff.select :blocked
@09:11:35.21 Plunge.seek :puckTop
@09:11:36.11 Air syringe pumping up to 8 strokes to reach 50psi
@09:11:36.12 Air stroke #1 of 8
@09:11:36.21 Air.in :compress
@09:11:36.27 Air.seek :evac
@09:11:36.40 10.3psi after compress
@09:11:46.98 10.7psi after compress (stable)
@09:11:47.00 Air.in :gulp
@09:11:47.07 Air.seek :topVent
@09:11:53.66 10.8psi after gulp
@09:11:59.23 10.9psi after gulp (stable)
@09:11:59.24 Air stroke #2 of 8
@09:11:59.34 Air.in :pressurize
@09:11:59.40 Air.seek :pressurized
@09:12:05.83 10.3psi after pressurize
@09:12:05.85 Air.in :compress
@09:12:05.92 Air.seek :evac
@09:12:12.95 23.7psi after compress
@09:12:25.52 28.3psi after compress (stable)
@09:12:25.54 Air.in :gulp
@09:12:25.97 Air.seek :topVent
@09:12:32.56 28.5psi after gulp
@09:12:38.13 28.5psi after gulp (stable)
@09:12:38.14 Air stroke #3 of 8
@09:12:38.24 Air.in :pressurize
@09:12:38.30 Air.seek :pressurized
@09:12:44.73 28.0psi after pressurize
@09:12:44.75 Air.in :compress
@09:12:44.82 Air.seek :evac
@09:12:51.84 38.2psi after compress
@09:13:04.41 42.9psi after compress (stable)
@09:13:04.43 Air.in :gulp
@09:13:04.51 Air.seek :topVent
@09:13:11.09 43.0psi after gulp
@09:13:16.67 43.1psi after gulp (stable)
@09:13:16.68 Air stroke #4 of 8
@09:13:16.77 Air.in :pressurize
@09:13:16.83 Air.seek :pressurized
@09:13:23.26 42.6psi after pressurize
@09:13:23.28 Air.in :compress
@09:13:23.35 Air.seek :evac
@09:13:30.51 Reached 51.0 of 50.0psi after pumping 7107uL (4.00 strokes)
@09:13:30.53 Air.in :default
@09:13:30.59 Air.hold
@09:13:30.67 Plunge.seek :puckBottom
@09:13:31.57 delay 3 seconds
@09:13:34.58 Handoff.select :waste
@09:13:34.71 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@09:13:50.29 delay 1:00
@09:14:50.32 Handoff.select :blocked
@09:14:50.45 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@09:15:13.02 Handoff.select :waste
@09:15:13.14 1.2psi at evacEnd
@09:15:13.15 Handoff.select :blocked
@09:15:13.29 Plunge.seek :puckTop
@09:15:14.18 Air.in :fast
@09:15:14.25 Air.seek :topVent
@09:15:20.89 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi
@09:15:27.47 Stable @15.2psi after Handoff blocked
@09:15:27.49 Air syringe pumping up to 8 strokes to reach 35psi
@09:15:27.50 Air stroke #1 of 8
@09:15:27.60 Air.in :pressurize
@09:15:27.66 Air.seek :pressurized
@09:15:34.09 14.9psi after pressurize
@09:15:34.11 Air.in :compress
@09:15:34.18 Air.seek :evac
@09:15:41.21 24.0psi after compress
@09:15:53.78 27.6psi after compress (stable)
@09:15:53.80 Air.in :gulp
@09:15:53.87 Air.seek :topVent
@09:16:00.46 27.6psi after gulp
@09:16:06.03 27.9psi after gulp (stable)
@09:16:06.04 Air stroke #2 of 8
@09:16:06.14 Air.in :pressurize
@09:16:06.19 Air.seek :pressurized
@09:16:12.63 27.5psi after pressurize
@09:16:12.65 Air.in :compress
@09:16:12.72 Air.seek :evac
@09:16:19.87 Reached 35.5 of 35.0psi after pumping 3553uL (2.00 strokes)
@09:16:19.89 Air.in :default
@09:16:19.96 Air.hold
@09:16:20.04 Plunge.seek :puckBottom
@09:16:20.93 delay 3 seconds
@09:16:23.95 Handoff.select :waste
@09:16:24.08 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@09:16:30.65 delay 1:00
@09:17:30.68 Handoff.select :blocked
@09:17:30.82 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@09:17:53.39 Handoff.select :waste
@09:17:53.51 1.9psi at evacEnd
@09:17:53.53 Plunge.seek :pTop
@09:17:54.43 delay 5 seconds
@09:17:59.46 Air.in :glide
@09:17:59.51 Air.seek :fullVent
@09:18:01.32 delay 2 seconds
@09:18:03.34 Plunge.seek :pBottom
@09:18:04.23 Seawater evacuated, Pressure above/below filter released.
@09:18:04.25 Plunge.seek :locked
@09:18:05.55 Handoff.select :waste
@09:18:05.65 Lysis1.in :slow
@09:18:05.71 Lysis1.seek 950uL
@09:18:20.24 delay 5 seconds
@09:18:25.26 Handoff.select :blocked
@09:18:25.40 Plunge.seek :puckTop
@09:18:26.32 Lysis1.seek 350uL
@09:18:54.49 delay 3 seconds
@09:18:57.57 Air.in mix with 2.32937 parts Lysis1
@09:18:58.01 <Airmixer> Air.seek :evac
@09:18:58.07 <PROCESSING> Lysis1.seek 190uL
@09:19:05.83 Air.in :fast
@09:19:05.89 Air.seek :full
@09:19:12.54 Lysis1.seek :empty
@09:19:21.67 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @22.7psi
@09:19:21.69 Plunge.seek :puckBottom
@09:19:22.69 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @18.6psi
@09:19:22.70 Polling Can every 2:00
@09:19:23.25 PuckHeater.configure :default #ambient 14.8C
@09:19:23.31 PuckHeater.seek 71.5C
@09:20:52.28 PuckHeater.hold 2:00,71.5C
@09:22:52.40 Polling Can every 2:00
@09:22:52.94 PuckHeater.configure :default #ambient 14.8C
@09:22:52.99 PuckHeater.seek 70.5C
@09:22:53.13 PuckHeater.hold 2:00,70.5C
@09:24:53.31 Polling Can every 2:00
@09:24:53.85 PuckHeater.configure :default #ambient 14.8C
@09:24:53.91 PuckHeater.hold 18:00,69.4C
@09:42:53.75 Polling Can every 2:00
@09:42:54.29 PuckHeater.configure :default #ambient 14.7C
@09:42:54.34 PuckHeater.seek 105.3C
@09:43:57.73 PuckHeater.hold 2:00,105.3C
@09:45:57.80 Polling Can every 2:00
@09:45:58.34 PuckHeater.configure :default #ambient 14.7C
@09:45:58.40 PuckHeater.seek 104.2C
@09:45:58.54 PuckHeater.hold 2:00,104.2C
@09:47:58.63 Polling Can every 2:00
@09:47:59.17 PuckHeater.configure :default #ambient 14.7C
@09:47:59.23 PuckHeater.hold 2:00,103.2C
@09:49:59.31 delay 5:00
@09:54:59.37 Lysate cooked @9.2psi
@09:54:59.39 Plunge.seek :puckTop
@09:55:00.29 Air syringe pumping up to 5 strokes to reach 40psi
@09:55:00.30 Air stroke #1 of 5
@09:55:00.40 Air.in :pressurize
@09:55:00.46 Air.seek :pressurized
@09:55:06.89 8.9psi after pressurize
@09:55:06.91 Air.in :compress
@09:55:06.98 Air.seek :evac
@09:55:12.71 Reached 41.2 of 40.0psi after pumping 1702uL (0.96 strokes)
@09:55:12.73 Air.in :default
@09:55:12.79 Air.hold
@09:55:12.87 delay 2 seconds
@09:55:14.90 Air.in :fast
@09:55:14.95 Air.seek :full
@09:55:21.32 Plunge.seek :puckBottom
@09:55:22.28 Lysis1.seek 138uL
@09:55:28.87 delay 2 seconds
@09:55:30.89 Plunge.seek :puckTop
@09:55:31.78 Lysis1.seek :empty
@09:55:38.39 Plunge.seek :puckBottom
@09:55:39.48 Lysis1.in :extract
@09:55:39.57 Lysis1.seek 675uL,nil
@09:55:41.90 <pump> Air.in :pressurize
@09:55:41.96 Air.seek :pressurized
@09:55:48.41 12.3psi after pressurize
@09:55:48.44 Air.in :compressLysate
@09:55:48.53 Air.seek :evac
@09:56:04.96 Reached 15.7 of 15.5psi after pumping 1620uL (0.91 strokes)
@09:56:04.98 Air.in :default
@09:56:05.04 Air.hold
@09:56:06.22 Air.in :compressLysate
@09:56:06.29 Air.seek :evac
@09:56:17.28 Air.in :default
@09:56:17.33 Air.hold
@09:56:17.52 <PROCESSING> Pulled 675uL lysate @13.4psi
@09:56:17.66 Lysis1.seek 800uL,nil
@09:56:17.84 <pump> Air.in :compressLysate
@09:56:17.91 Air.seek :evac
@09:56:18.28 14.8psi after compress
@09:56:28.85 13.1psi after compress (stable)
@09:56:33.98 Air.in :default
@09:56:34.09 Air.hold
@09:56:34.17 <PROCESSING> Lysis1.in :default
@09:56:34.25 Lysis1.hold
@09:56:34.50 Air.in :glide
@09:56:34.55 Air.seek :fullVent
@09:56:36.35 Handoff.select :waste
@09:56:36.49 Recovered 687uL lysate @13.5psi
@09:56:36.50 delay 3 seconds
@09:56:39.58 After 3 delay Recovered 687uL lysate @-0.0psi
@09:56:40.71 <LRAUV> -> Cmd.stop
@09:56:40.81 <closeHandoff> Handoff.select :close
@09:56:40.95 <LRAUV> Gate.power :heat,:OFF
@09:56:40.97 Gate.power :cartridge,:OFF
@09:56:41.06 PinPort.in :unlocking
@09:56:41.12 PinPort.seek :clear
@09:56:43.55 Carousel.in :default
@09:56:43.66 Carousel.home.seek 52
@09:56:43.97 Plunge.seek :locked
@09:56:45.34 <Archive> Archive.in :unlocking
@09:56:45.46 Archive.seek :clear
@09:56:45.51 <Air> Air.in :unlocking
@09:56:45.71 Air.seek :clear
@09:56:45.81 <Lysis1> Lysis1.in :unlocking
@09:56:45.95 <Lysis2> Lysis2.in :unlocking
@09:56:45.97 <Lysis1> Lysis1.seek :clear
@09:56:46.08 <Lysis2> Lysis2.seek :clear
@09:56:50.89 <LRAUV> Gate.power :motor,:OFF
@09:56:50.91 Gate.power :analytic1,:OFF
@09:56:50.93 Gate.power :analytic2,:OFF
@09:56:50.95 Gate.power :analytic3,:OFF
@09:56:50.98 Gate.power :core,:OFF
@09:56:51.00 Gate.power :main,:OFF
@09:56:51.03 Safely stopped and ready to power off
:STOPPED