@17:43:49.23PDT22-Jul-19
>BREAK< ESP3 Gateway v0.99 for core bus @17:43:49.25 >BREAK< ESP3 Gateway v0.99 for cartridge bus @17:43:50.85 Configuration for honu by brent@mbari.org 4/4/19 /home/esp/esp2/type/auv/honu/configure.rb @17:43:53.12 Polling Can every 5:00 @17:43:53.43 Clients may connect on TCP port 9999 @17:43:53.44 Connecting to 10.89.5.2:9999 @17:43:53.48 Waiting for espclients... @17:43:53.82 Session Begun @17:43:55.79 -> showStatus @17:43:57.80 -> Cmd.loadCartridge -9 @17:43:57.93 Gate.power :main,:ON,limit to 5000mA @17:43:57.94 Gate.power :analytic1,:ON,limit to 5000mA @17:43:57.97 Gate.power :analytic2,:ON,limit to 5000mA @17:43:57.99 Gate.power :analytic3,:ON,limit to 5000mA @17:43:58.12 Powering core bus @17:44:00.40 SPR Module 1, FW Rev. B, FPGA Rev. C @17:44:00.75 Archive.configure :default @17:44:00.90 Air.configure :default @17:44:01.00 Lysis1.configure :default @17:44:01.10 Lysis2.configure :default @17:44:01.22 Plunge.configure :default @17:44:01.34 SamplePump.configure :default @17:44:01.46 ExtraPump.configure :default @17:44:01.58 Toroid.configure :default @17:44:01.70 Carousel.configure :default @17:44:01.82 Pressure.configure :default @17:44:01.93 PinPort.configure :default @17:44:01.98 All elf controllers running firmware version 4.58 @17:44:02.01 Gate.power :motor,:ON,limit to 5000mA @17:44:02.17 Archive.reconfigure :default @17:44:02.50 Toroid.reconfigure :default @17:44:02.57 Plunge.reconfigure :default @17:44:02.60 Lysis1.reconfigure :default @17:44:02.65 PinPort.reconfigure :default @17:44:02.68 Lysis2.reconfigure :default @17:44:02.72 Air.reconfigure :default @17:44:03.09 Air.home.jog 0 @17:44:03.14 PinPort.home.jog 0 @17:44:03.20 Plunge.home.jog 0 @17:44:03.25 Lysis2.home.jog 0 @17:44:03.31 Lysis1.home.jog 0 @17:44:03.37 Toroid.home.jog 0 @17:44:03.43 Archive.home.jog 0 @17:44:03.97 Toroid.reconfigure :homing @17:44:04.01 Plunge.reconfigure :homing @17:44:04.04 Air.reconfigure :homing @17:44:04.08 PinPort.reconfigure :homing @17:44:04.12 Lysis1.reconfigure :homing @17:44:04.16 Archive.reconfigure :homing @17:44:04.20 Lysis2.reconfigure :homing @17:44:04.45 Archive.jog 1360 @17:44:04.50 Toroid.jog 641 @17:44:04.55 Plunge.jog 1535 @17:44:04.61 Lysis1.jog 1411 @17:44:04.66 Lysis2.jog 169 @17:44:04.72 Air.jog 1397 @17:44:04.79 PinPort.jog -108 @17:44:05.02 PinPort.setRawPosition! 0 @17:44:05.06 PinPort.seek :home @17:44:05.16 PinPort.reconfigure :default @17:44:05.19 Lysis2.setRawPosition! 50 @17:44:05.27 Lysis2.seek :home @17:44:05.57 Lysis2.reconfigure :default @17:44:05.64 Archive.setRawPosition! 50 @17:44:05.69 Archive.seek :home @17:44:05.79 Plunge.setRawPosition! 50 @17:44:05.82 Lysis1.setRawPosition! 50 @17:44:05.92 Lysis1.seek :home @17:44:05.95 Plunge.seek :home @17:44:06.09 Air.setRawPosition! 50 @17:44:06.11 Archive.reconfigure :default @17:44:06.19 Air.seek :home @17:44:06.34 Plunge.reconfigure :default @17:44:06.37 Lysis1.reconfigure :default @17:44:06.54 Air.reconfigure :default @17:44:06.67 Toroid.setRawPosition! 0 @17:44:06.72 Toroid.seek :home @17:44:06.81 Toroid.reconfigure :default @17:44:06.94 Toroid.seek :clear @17:44:08.56 Carousel.reconfigure :default @17:44:08.63 Carousel.home.jog 0 @17:44:09.13 Carousel.seek 22 @17:44:09.27 Selecting Cartridge 21 @17:44:09.52 Carousel.seek 21 @17:44:10.00 Lysis1.seek :locked @17:44:14.13 Plunge.seek :locked @17:44:14.25 Air.seek :locked @17:44:18.38 Powering cartridge bus @17:44:20.50 Gate.setIR 1750,5000,0,6 @17:44:20.63 Gate.setIR 0,5000,0,6 @17:44:20.68 Loaded Lyse-n-Go Cartridge @17:44:20.70 PuckHeater.configure HeatConfig @17:44:20.75 Gate.power :heat,:ON,limit to 5000mA @17:44:36.53 -> Cmd.startFiltering @17:44:36.75 Duration of filtering limited to 2:05:00 @17:44:37.01 Priming sample loop w/100ml, bypass w/10ml @17:44:37.02 SamplePump.setPosition! 0ml @17:44:37.06 Bypass.open @17:44:38.51 Intake.open @17:44:39.33 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:44:42.17 Vacuum tare is 52% of full scale @17:44:42.29 Pressure.reconfigure :default @17:44:42.35 Pressure.hold @17:44:42.51 SamplePump.reconfigure :prime @17:44:42.59 SamplePump.seek 10ml,1:10 @17:44:51.54 Pressure.coast @17:44:51.59 SamplePump.setPosition! 0ml @17:44:51.64 Exhaust.open @17:44:53.08 Bypass.close @17:44:54.58 Pressure.reconfigure :default @17:44:54.64 Pressure.hold @17:44:55.23 SamplePump.reconfigure :prime @17:44:55.31 SamplePump.seek 100ml,7:10 @17:46:39.55 Pressure.coast @17:46:39.61 Bypass.open @17:46:40.30 Intake.close @17:46:41.16 Toroid.reconfigure :hold @17:46:41.23 Toroid.seek :crack @17:46:42.02 Toroid.reconfigure :default @17:46:42.08 Toroid.seek :clear @17:46:43.64 Toroid.reconfigure :hold @17:46:43.71 Toroid.seek :cartridge @17:46:45.52 Toroid.reconfigure :default @17:46:45.59 Toroid.seek :clear @17:46:47.56 Pressurizing cartridge to 14psi above 12.2psia @17:46:47.57 SamplePump.setPosition! 0ml @17:46:47.61 Intake.open @17:46:47.90 Bypass.close @17:46:48.59 Exhaust.close @17:46:49.42 Pressure.reconfigure :default @17:46:49.47 Pressure.hold @17:46:49.64 SamplePump.reconfigure :charge @17:46:49.71 SamplePump.seek 2.3ml,41 seconds @17:47:02.42 Pressure.coast @17:47:02.47 Intake.close @17:47:03.27 Pressurized cartridge with 0.760ml to 14.1psi @17:47:03.28 Isolated @23.8psia (11.7psi) @17:47:03.29 Checking for cartridge leaks from 23.8psia @17:47:03.33 Waiting up to 20 seconds for cartridge pressure to stabilize +/-0.75psi @17:47:08.36 Cartridge pressure fell 1% to 23.6psia @17:47:08.44 Sampling 1000.0ml @17:47:08.45 SamplePump.setPosition! 0ml @17:47:08.51 Exhaust.open @17:47:09.22 Intake.open @17:47:10.07 Pressure.reconfigure :default @17:47:10.13 Pressure.hold @17:47:10.34 SamplePump.reconfigure :sample @17:47:10.42 SamplePump.seek 1000ml,1:07:09 @17:47:13.10 Cartridge 21 sampling 1/1000ml at 0.47ml/s with 17.7psi vacuum @17:48:13.11 Cartridge 21 sampling 25/1000ml at 0.37ml/s with 14.5psi vacuum @17:49:13.10 Cartridge 21 sampling 48/1000ml at 0.40ml/s with 12.1psi vacuum @17:50:13.11 Cartridge 21 sampling 72/1000ml at 0.42ml/s with 14.0psi vacuum @17:51:13.11 Cartridge 21 sampling 96/1000ml at 0.41ml/s with 12.2psi vacuum @17:52:13.11 Cartridge 21 sampling 120/1000ml at 0.39ml/s with 11.7psi vacuum @17:53:13.11 Cartridge 21 sampling 144/1000ml at 0.42ml/s with 13.4psi vacuum @17:54:13.10 Cartridge 21 sampling 169/1000ml at 0.42ml/s with 13.6psi vacuum @17:55:13.11 Cartridge 21 sampling 193/1000ml at 0.40ml/s with 15.1psi vacuum @17:56:13.11 Cartridge 21 sampling 217/1000ml at 0.42ml/s with 13.7psi vacuum @17:57:13.11 Cartridge 21 sampling 242/1000ml at 0.42ml/s with 14.0psi vacuum @17:58:13.11 Cartridge 21 sampling 267/1000ml at 0.41ml/s with 12.5psi vacuum @17:59:13.10 Cartridge 21 sampling 292/1000ml at 0.41ml/s with 13.5psi vacuum @18:00:13.11 Cartridge 21 sampling 317/1000ml at 0.40ml/s with 13.0psi vacuum @18:01:13.11 Cartridge 21 sampling 342/1000ml at 0.43ml/s with 13.2psi vacuum @18:02:13.11 Cartridge 21 sampling 367/1000ml at 0.42ml/s with 12.6psi vacuum @18:03:13.11 Cartridge 21 sampling 392/1000ml at 0.42ml/s with 13.1psi vacuum @18:04:13.10 Cartridge 21 sampling 417/1000ml at 0.39ml/s with 12.4psi vacuum @18:05:13.11 Cartridge 21 sampling 442/1000ml at 0.42ml/s with 11.7psi vacuum @18:06:13.11 Cartridge 21 sampling 467/1000ml at 0.42ml/s with 14.9psi vacuum @18:07:13.11 Cartridge 21 sampling 492/1000ml at 0.41ml/s with 12.5psi vacuum @18:08:13.11 Cartridge 21 sampling 517/1000ml at 0.43ml/s with 13.6psi vacuum @18:09:13.10 Cartridge 21 sampling 543/1000ml at 0.41ml/s with 12.2psi vacuum @18:10:13.11 Cartridge 21 sampling 568/1000ml at 0.40ml/s with 13.1psi vacuum @18:11:13.11 Cartridge 21 sampling 593/1000ml at 0.42ml/s with 11.6psi vacuum @18:12:13.11 Cartridge 21 sampling 618/1000ml at 0.42ml/s with 11.1psi vacuum @18:13:13.11 Cartridge 21 sampling 643/1000ml at 0.42ml/s with 12.3psi vacuum @18:14:13.11 Cartridge 21 sampling 668/1000ml at 0.42ml/s with 14.2psi vacuum @18:15:13.11 Cartridge 21 sampling 693/1000ml at 0.41ml/s with 14.6psi vacuum @18:16:13.11 Cartridge 21 sampling 718/1000ml at 0.42ml/s with 13.6psi vacuum @18:17:13.11 Cartridge 21 sampling 744/1000ml at 0.42ml/s with 14.8psi vacuum @18:18:13.11 Cartridge 21 sampling 769/1000ml at 0.43ml/s with 13.5psi vacuum @18:19:13.11 Cartridge 21 sampling 794/1000ml at 0.40ml/s with 14.8psi vacuum @18:20:13.11 Cartridge 21 sampling 818/1000ml at 0.43ml/s with 14.2psi vacuum @18:21:13.11 Cartridge 21 sampling 844/1000ml at 0.42ml/s with 14.4psi vacuum @18:22:13.11 Cartridge 21 sampling 869/1000ml at 0.42ml/s with 12.5psi vacuum @18:23:13.11 Cartridge 21 sampling 895/1000ml at 0.42ml/s with 14.4psi vacuum @18:24:13.11 Cartridge 21 sampling 920/1000ml at 0.42ml/s with 13.1psi vacuum @18:25:13.11 Cartridge 21 sampling 946/1000ml at 0.42ml/s with 12.5psi vacuum @18:26:13.11 Cartridge 21 sampling 972/1000ml at 0.43ml/s with 13.0psi vacuum @18:27:13.11 Cartridge 21 sampling 998/1000ml at 0.44ml/s with 14.6psi vacuum @18:27:18.91 Pressure.coast @18:27:19.02 Intake.close @18:27:19.06 Exhaust.close @18:27:19.23 Toroid.reconfigure :hold @18:27:19.30 Toroid.seek :bypass @18:27:20.92 Toroid.reconfigure :default @18:27:20.98 Toroid.seek :clear @18:27:22.61 Sampled 1000.0ml @18:27:24.10 -> Cmd.startProcessing @18:27:25.96 SPR.command "read_sequences" @18:27:26.06 SPR.command "stats_on" @18:27:26.10 SPR.command "start 0 1" @18:27:28.11 Begun log /var/log/esp/spr19jul2218h.spr @18:27:28.91 Handoff.select :waste @18:27:29.06 Archive.seek :empty @18:27:36.32 Syringe tare is 19% of full scale @18:27:46.85 PinPort.seek :cartridge @18:27:51.41 Lysis1.seek 1350ul @18:27:53.89 Lysis2.seek 1700ul @18:27:56.98 Plunge.seek :reagent @18:27:57.81 Evacuating puck with filter bubble point of 30psi @18:27:57.83 PinPort.seek :cartridge @18:27:57.91 Handoff.select :waste @18:28:05.22 Air pumping 1 strokes to reach 4psi @18:28:05.24 Air.reconfigure :gulp @18:28:05.32 Air.seek :tVent @18:28:07.13 0.4psi after gulp @18:28:07.14 Air stroke #1 of 1 @18:28:07.16 Air.reconfigure :pressurize @18:28:07.25 Air.seek :pressurized @18:28:20.09 0.2psi after pressurize @18:28:20.10 Air.reconfigure :compress @18:28:20.18 Air.seek :evac @18:28:22.84 0.3psi after compress @18:28:36.72 0.2psi after compress (stable) @18:28:36.84 at 0.2psi Opened to waste @18:28:36.85 Handoff.select :blocked @18:28:37.02 Air pumping 10 strokes to reach 30psi @18:28:37.04 Air.reconfigure :gulp @18:28:37.11 Air.seek :tVent @18:28:47.02 9.0psi after gulp @18:28:47.03 Air stroke #1 of 10 @18:28:47.05 Air.reconfigure :pressurize @18:28:47.14 Air.seek :pressurized @18:28:59.97 12.1psi after pressurize @18:28:59.98 Air.reconfigure :compress @18:29:00.06 Air.seek :evac @18:29:02.73 14.4psi after compress @18:29:11.66 Reached 30.3 of 30.0psi after pumping 3115ul (2.00 strokes) @18:29:11.68 Handoff.select :waste @18:29:20.13 delay 1:00 @18:30:20.16 Handoff.select :blocked @18:30:43.56 Pressure too High, open to waste again (4.9psi) @18:30:43.57 Handoff.select :waste @18:30:43.69 delay 1:00 @18:31:43.71 Handoff.select :blocked @18:32:08.72 Handoff.select :waste @18:32:08.84 PSI at @2.88psi at evacEnd @18:32:08.85 Handoff.select :blocked @18:32:08.97 Air.reconfigure :gulp @18:32:09.05 Air.seek :tVent @18:32:26.51 Stable @2.2psi after Handoff blocked @18:32:26.60 Air pumping 10 strokes to reach 27psi @18:32:26.61 Air stroke #1 of 10 @18:32:26.63 Air.reconfigure :pressurize @18:32:26.71 Air.seek :pressurized @18:32:39.55 2.6psi after pressurize @18:32:39.57 Air.reconfigure :compress @18:32:39.66 Air.seek :evac @18:32:42.33 3.4psi after compress @18:33:10.07 14.5psi after compress (stable) @18:33:10.09 Air.reconfigure :gulp @18:33:10.17 Air.seek :tVent @18:33:20.06 15.0psi after gulp @18:33:20.07 Air stroke #2 of 10 @18:33:20.09 Air.reconfigure :pressurize @18:33:20.17 Air.seek :pressurized @18:33:33.01 15.1psi after pressurize @18:33:33.03 Air.reconfigure :compress @18:33:33.10 Air.seek :evac @18:33:35.78 15.6psi after compress @18:34:01.66 25.2psi after compress (stable) @18:34:01.68 Air.reconfigure :gulp @18:34:01.76 Air.seek :tVent @18:34:11.65 25.9psi after gulp @18:34:11.67 Air stroke #3 of 10 @18:34:11.69 Air.reconfigure :pressurize @18:34:11.77 Air.seek :pressurized @18:34:24.62 25.8psi after pressurize @18:34:24.65 Air.reconfigure :compress @18:34:24.73 Air.seek :evac @18:34:27.40 26.1psi after compress @18:34:28.68 Reached 27.3 of 27.2psi after pumping 6230ul (4.00 strokes) @18:34:28.69 Handoff.select :waste @18:34:36.59 delay 1:00 @18:35:36.61 Handoff.select :blocked @18:36:01.62 Handoff.select :waste @18:36:01.74 PSI at @1.48psi at evacEnd @18:36:01.75 Air.reconfigure :default @18:36:01.84 Air.seek :fVent @18:36:03.27 delay 5 seconds @18:36:08.29 Seawater evacuated, Presure above/below filter released. Lysis begins @18:36:08.36 Lysis1.reconfigure :slow @18:36:08.43 Lysis1.seek 1050ul @18:36:38.46 delay 5 seconds @18:36:43.48 Handoff.select :blocked @18:36:43.64 Lysis1.seek 450ul @18:37:43.65 delay 3 seconds @18:37:46.73 Air.reconfigure to mix into 1.37806 parts Lysis1 @18:37:47.26 Air.seek :evac @18:37:47.31 Lysis1.seek 300ul @18:38:02.42 Air.reconfigure :default @18:38:02.48 Air.seek :full @18:38:16.71 Lysis1.seek :empty @18:38:46.81 Lysis Delivered. Syringe pressure @45.6psi @18:38:46.85 PuckHeater.seek 84.3C @18:39:36.38 PuckHeater.hold 15:00,84.3C @18:54:34.61 Lysis complete. Syringe pressure @33.3psi @18:54:34.62 Handoff.select :blocked @18:54:34.72 Lysis1.reconfigure :halfSpeed @18:54:34.79 Lysis1.seek 200ul @18:54:38.55 delay 1 second @18:54:39.57 Lysis1.seek :empty @18:54:43.39 Initial AirS pressure @34.5psi @18:54:43.45 Lysis1.reconfigure :extract @18:54:43.54 Lysis1.seek 875ul,10:00 @18:54:44.70 Air pumping 10 strokes to reach 21psi @18:54:44.73 Air stroke #1 of 10 @18:54:44.76 Air.reconfigure :pressurize @18:54:44.84 Air.seek :pressurized @18:54:57.67 14.4psi after pressurize @18:54:57.68 Air.reconfigure :compress @18:54:57.76 Air.seek :evac @18:55:00.42 19.0psi after compress @18:55:14.67 15.6psi after compress (stable) @18:55:14.68 Air.reconfigure :gulp @18:55:14.76 Air.seek :tVent @18:55:24.64 14.6psi after gulp @18:55:24.65 Air stroke #2 of 10 @18:55:24.67 Air.reconfigure :pressurize @18:55:24.75 Air.seek :pressurized @18:55:37.59 15.4psi after pressurize @18:55:37.60 Air.reconfigure :compress @18:55:37.68 Air.seek :evac @18:55:40.37 15.5psi after compress @18:55:54.22 15.7psi after compress (stable) @18:55:54.24 Air.reconfigure :gulp @18:55:54.31 Air.seek :tVent @18:56:04.00 Air.reconfigure :default @18:56:04.06 Air.hold @18:56:04.30 Lysis1 at 875ul pressure @15.9psi @18:56:04.31 Lysis1.reconfigure :default @18:56:04.39 Lysis1.seek 900ul @18:56:05.17 Lysis1 at 900ul pressure(finPSI) @10.8psi @18:56:05.18 delay 1 second @18:56:06.29 Lysis1 at 900ul pressure @12.2psi @18:56:06.31 Air.reconfigure :halfSpeed @18:56:06.40 Air.seek :fVent @18:56:36.10 Puck Top Vented. Syringe pressure @5.9psi @18:56:36.11 Handoff.select :waste @18:56:36.23 delay 5 seconds @18:56:41.25 Lysate Recovered in LyS1 @18:56:41.26 Lysis1.reconfigure :DAmix @18:56:41.37 Lysis2.reconfigure :DAmix @18:56:41.80 Lysis1.seek 650ul @18:56:41.88 Lysis2.seek 1450ul @18:57:16.90 delay 5 seconds @18:57:21.92 Handoff.select SPR @18:57:22.03 SPR.command "start 2 14" @18:57:23.75 delay 10 seconds @18:57:33.77 SPR.command "start 1 0" @18:57:36.51 Waiting up to 4:00 for SPR to enter state 1 @19:00:09.17 SPR.command "hold_on" @19:00:10.56 SPR Dilution 1 of 1 -- Running Experiment 1 @19:00:10.97 Lysis1.seek 350ul @19:00:11.01 Lysis2.seek 1150ul @19:00:53.08 volL1 = 300.0 and volL2 = 300.0 @19:00:53.09 delay 15 seconds @19:01:08.12 SPR.command "hold_off" @19:01:11.21 Waiting up to 25:00 for SPR to enter state 7 @19:21:25.10 SPR Analysis has Completed @19:21:25.12 Handoff.select :waste @19:21:26.03 Ended log /var/log/esp/spr19jul2218h.spr @19:21:26.04 SPR.command "stats_off" @19:21:27.36 -> Cmd.stop @19:21:27.45 Handoff.select :close @19:21:27.57 Gate.power :heat,:OFF @19:21:27.59 Gate.power :cartridge,:OFF @19:21:27.67 Carousel.seek 21 @19:21:27.84 Plunge.seek :locked @19:21:28.68 PinPort.seek :clear @19:21:33.29 Archive.seek :clear @19:21:33.41 Air.reconfigure :default @19:21:33.54 Lysis1.reconfigure :default @19:21:33.66 Air.seek :clear @19:21:33.72 Lysis1.seek :clear @19:21:33.80 Lysis2.reconfigure :default @19:21:33.87 Lysis2.seek :clear @19:21:51.04 Archive.coast @19:21:51.09 Air.coast @19:21:51.14 Lysis1.coast @19:21:51.19 Lysis2.coast @19:21:51.24 Plunge.coast @19:21:51.63 SamplePump.coast @19:21:51.69 Toroid.coast @19:21:51.74 Carousel.coast @19:21:51.79 PinPort.coast @19:21:51.97 Gate.power :motor,:OFF @19:21:51.98 Gate.power :core,:OFF @19:21:52.01 Gate.power :analytic1,:OFF @19:21:52.03 Gate.power :analytic2,:OFF @19:21:52.05 Gate.power :analytic3,:OFF @19:21:52.07 Gate.power :main,:OFF @19:21:52.09 Safely stopped and ready to power off