+ ESPwhich standAloneWCR_4.rb + fn=standAloneWCR_4.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/standAloneWCR_4.rb + [ -f /home/esp/esp2/mission/standAloneWCR_4.rb ] + realpath /home/esp/esp2/mission/standAloneWCR_4.rb + return 0 + scriptPath=/home/esp/esp2local/mission/standAloneWCR_4.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/standAloneWCR_4.rb @16:40:05.01UTC19-Aug-25
ESP2 v3.42 I2C Gateway v3.20 for core bus @16:40:07.67 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @16:40:11.25 Polling Can every 10:00 @16:40:11.30 Loading /home/esp/esp2local/mission/standAloneWCR_4.rb mission @16:40:11.35 Preparing to sleep until + 00:00:05 @16:40:11.37 GoodNews.email "Starting standAloneWCR_4 w/pucks in tube 2\n{2 => 1}\nCTD data unavailable\nCan@16:40:11, 40% flow, 25.7C, 38% humidity, 14.9psia, 13.73V, 0.259A avg, 5.430Ah, 3.55W",Subject: "standAloneWCR_4 begun" @16:40:11.43
Asleep til + 00:00:05 @16:40:11.45 Continuing at 16:40 @16:40:11.71 Gate.power :core,:OFF @16:40:11.72 Gate.power :raw,:OFF @16:40:11.73 Shutdown @16:40:14.43 GoodNews.email "Then, in tube 2: initialPurge; standAloneWCR([nil,50], $da)\n/home/esp/esp2local/mission/standAloneWCR_4.rb:10\n{2 => 1}\nCTD data unavailable\nCan@16:40:11, 40% flow, 25.7C, 38% humidity, 14.9psia, 13.73V, 0.265A avg, 5.430Ah, 3.64W",Subject: "Asleep until 16:40" @16:40:16.10 upsync @16:40:16.34
Powering core bus @16:40:18.71 upsync @16:40:22.06
Gate.power :raw,:ON @16:40:22.81 CS.configure :default @16:40:22.86 PS.configure :default @16:40:22.91 SS.configure :default @16:40:22.95 CC.configure :default @16:40:23.00 PC.configure :default @16:40:23.07 Forearm.configure :loose @16:40:23.11 Elbow.configure :loose @16:40:23.18 SC.configure :loose @16:40:23.23 SE.configure :default @16:40:23.27 CH.configure :default @16:40:23.31 PH.configure :default @16:40:23.35 All dwarf controllers running firmware version 3.85 @16:40:23.46 Valve.reset @16:40:23.68 Intake.select :close @16:40:23.74 Exhaust.select :close @16:40:23.77 CTV.dial :air @16:40:23.85 PSV.dial :air @16:40:23.90 CSV.dial :waste1 @16:40:24.03
Forearm.home.seek :retract @16:40:24.10 SSV.dial :spit,avoiding: :exhaust @16:40:24.19 PS.home.seek 0ml @16:40:24.35 CS.reconfigure :fast @16:40:24.38 SS.home.seek 0ml @16:40:24.49 CS.home.seek 0ml @16:40:24.81 Hand.close @16:40:24.84
Elbow.reconfigure :loose @16:40:24.93 Elbow.jog 1500 @16:40:25.50 Elbow.reconfigure :loose @16:40:25.54 Elbow.home.seek :clear @16:40:29.93 SE.home.seek :down @16:40:30.59 SC.reconfigure :tight @16:40:30.63 SC.home.seek :initial @16:40:33.35 PS.stop @16:40:56.22
SC.configure :limp @16:40:56.27 Forearm.seek :retract @16:40:56.34 Elbow.seek :hammer @16:40:57.42 Forearm.reconfigure :tight @16:40:57.47 Forearm.seek retracted @16:40:58.02 PC.home.seek :open @16:40:58.03
CC.home.seek :open @16:41:01.16 Phase [initialPurge; standAloneWCR([nil,50], $da)] @16:41:01.17 Notice.email "At 16:41:01 began in tube 2: initialPurge; standAloneWCR([nil,50], $da)\n/home/esp/esp2local/mission/standAloneWCR_4.rb:10\n{2 => 1}\nCTD data unavailable\nCan@16:41:01, 40% flow, 25.7C, 38% humidity, 14.9psia, 13.69V, 0.246A avg, 5.436Ah, 3.37W",Subject: "Started standAloneWCR_4 phase initialPurge; standAloneWCR([nil,50], $da)" @16:41:01.19
Starting initialPurge @16:41:01.23 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:41:01.29 Hand.open @16:41:02.15 Elbow.reconfigure :tight @16:41:02.20 Elbow.seek FlushPuck::Garage @16:41:04.20 Elbow.reconfigure :loose @16:41:04.42 Forearm.reconfigure :loose @16:41:04.49 Forearm.seek :garage @16:41:05.55 Hand.close @16:41:05.70 upsync @16:41:06.41
Forearm.seek :retract @16:41:07.46 Elbow.reconfigure :tight @16:41:07.50 Elbow.seek CC @16:41:10.35 Elbow.reconfigure :loose @16:41:10.45 Forearm.reconfigure :tight @16:41:10.50 Forearm.seek CC @16:41:11.86 CC.seek :closed @16:41:15.92 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.06 @16:41:16.04 Hand.open @16:41:16.85 Forearm.reconfigure :loose @16:41:16.89 Forearm.seek :retract @16:41:17.89 Flushing the Collection Valve::Manifold @16:41:18.02 CSV.dial CSR @16:41:19.30 Pulling 5ml of CSR.flush CSR.select :flush @16:41:19.42 CS.reconfigure :default @16:41:19.47 CS.seek 5ml @16:41:33.47 delay 5 seconds @16:41:38.54 CSR.select :air @16:41:38.71 CS.reconfigure :fast @16:41:38.75 CS.seek 7.5ml @16:41:45.37 CSV.dial :waste @16:41:46.64 CS.reconfigure :default @16:41:46.69 CS.seek 0ml @16:42:06.87 Purging external Intake @16:42:06.99 CSV.dial :flush @16:42:07.42 CS.reconfigure :fast @16:42:07.47 CS.seek 10ml @16:42:31.12 delay 5 seconds @16:42:36.14 CSV.dial CBV @16:42:36.18
CBV.dial CSV @16:42:37.00 CTV.dial :intake @16:42:37.78 Intake.select :open @16:42:37.89 CS.seek 0ml @16:43:01.59 Intake.select :close @16:43:01.69 CTV.dial :air @16:43:02.47 Flushing upper manifold @16:43:02.59 CSV.dial :flush @16:43:03.32 CS.seek 5ml @16:43:15.56 delay 5 seconds @16:43:20.58 CSV.dial :air @16:43:21.09 CS.seek 7ml @16:43:26.50 CSV.dial CBV @16:43:26.53
CBV.dial CSV @16:43:27.54 CTV.dial :air @16:43:27.63 CS.seek 0ml @16:43:44.34 delay 7 seconds @16:43:51.37 CS.seek 3ml @16:43:59.15 CSV.dial :waste @16:43:59.61 CS.seek 0ml @16:44:07.41 CSV.dial :flush @16:44:07.86 CS.seek 3ml @16:44:15.65 delay 5 seconds @16:44:20.67 CSV.dial :air @16:44:21.18 CS.seek 5ml @16:44:26.62 CSV.dial :air @16:44:26.70 CS.seek 0ml @16:44:38.87 delay 7 seconds @16:44:45.90 CS.seek 3ml @16:44:53.68 CSV.dial :waste @16:44:54.41 CS.seek 0ml @16:45:02.09 Garaging FlushPuck in Collection Clamp @16:45:02.16 Elbow.reconfigure :tight @16:45:02.21 Elbow.seek CC @16:45:02.42 Elbow.reconfigure :loose @16:45:02.52 Forearm.seek CC @16:45:03.53 Hand.close @16:45:04.28 CC.seek :open @16:45:08.76 Forearm.seek :retract @16:45:09.75 Elbow.reconfigure :tight @16:45:09.80 Elbow.seek :garage @16:45:12.61 Elbow.reconfigure :loose @16:45:12.72 Forearm.reconfigure :tight @16:45:12.76 Forearm.seek :garage @16:45:13.90 Hand.open @16:45:14.72 Forearm.reconfigure :loose @16:45:14.76 Forearm.seek :retract @16:45:15.81 Starting stand-alone da WCR @16:45:15.82 da wcr sampling at most 50ml @16:45:15.85 Whole Cell Archive sampling 50ml @16:45:15.86 DAfans.email "Started stand-alone da WCR in tube 2 at 16:45:15UTC19-Aug-25\n{2 => 1}\nCTD data unavailable\nCan@16:41:01, 40% flow, 25.7C, 38% humidity, 14.9psia, 13.69V, 0.246A avg, 5.436Ah, 3.37W",Subject: "da wcr sampling at most 50ml" @16:45:15.88 Loading wcr puck from tube 2 into Collection Clamp @16:45:16.15 SC.reconfigure :tight @16:45:16.22 SC.seek 2 @16:45:20.19 upsync @16:45:23.36 SC.configure :limp @16:45:23.60 Elbow.reconfigure :tight @16:45:23.65 Elbow.seek 2 @16:45:25.55 Forearm.reconfigure :tight @16:45:25.60 Forearm.seek 2 + 800 counts @16:45:26.62 SE.reconfigure :pick @16:45:26.68 SE.seek :beamBreak @16:45:29.56 SE.configure :default @16:45:29.73 Elbow.seek 2 @16:45:29.92 Forearm.seek 2 + 300 counts @16:45:30.23 Forearm.reconfigure :loose @16:45:30.33 Hand.close @16:45:31.04 SE.seek :down @16:45:31.68 Forearm.seek :retract @16:45:32.65 Elbow.seek CC @16:45:34.26 Elbow.reconfigure :loose @16:45:34.34 SE.stop @16:45:34.45 Forearm.reconfigure :tight @16:45:34.50 Forearm.seek CC @16:45:35.81 CC.seek :closed @16:45:39.78 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.98 @16:45:39.80 Hand.open @16:45:40.59 Forearm.reconfigure :loose @16:45:40.63 Forearm.seek :retract @16:45:41.75 collectSample 50ml w/bubble point of 35psi @16:45:41.76 Priming 7ml @16:45:41.77 Intake.select :close @16:45:41.79 Exhaust.select :close @16:45:41.80 SS.hold @16:45:42.00 Calibrating pressure and temperature sensors @16:45:42.03 CTV.dial :air @16:45:42.05 SSV.dial :spit,avoiding: :exhaust @16:45:42.13 SS.reconfigure :fast @16:45:42.19 SS.seek 5ml @16:45:45.13 SSV.dial CBV @16:45:45.17 CBV.dial SSV @16:45:46.50 delay 3.8 seconds @16:45:50.43 Sensor limits: Intake max 81.3psia; Outlet max 106.0psia @16:45:50.44 SSV.dial :spit @16:45:51.78 SS.seek 0ml @16:45:54.80 SSV.dial CBV @16:45:56.14 CTV.dial :intake @16:45:56.90 SSV.dial CBV @16:45:56.93 CBV.dial SSV @16:45:57.16 Allowing 40s for 7.0ml of remaining 7.0ml @16:45:57.18 Intake.select :open @16:45:59.34 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:45:59.36 SS.reconfigure :inhaling 2.17ml/s @16:45:59.41 SS.seek 7ml @16:46:06.09 Stroke completed -- waiting for pressure to equalize ... @16:46:06.11 SS.reconfigure :exhale @16:46:06.15 SS.hold @16:46:08.04 Intake.select :close @16:46:08.20 SSV.dial :exhaust @16:46:08.97 Exhaust.select :open @16:46:09.09 SS.seek 0ml @16:46:12.86 Exhaust.select :close @16:46:12.97 Sampling 50.0ml @16:46:12.98 Intake.select :close @16:46:12.99 Exhaust.select :close @16:46:13.00 SS.reconfigure :default @16:46:13.05 SS.hold @16:46:13.26 CTV.dial :intake @16:46:13.43 SSV.dial CBV @16:46:13.46 CBV.dial SSV @16:46:14.25 Allowing 130s for 25.0ml of remaining 50.0ml @16:46:14.27 Intake.select :open @16:46:16.43 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:46:16.45 SS.reconfigure :inhaling 2.17ml/s @16:46:16.50 SS.seek 25ml @16:46:32.64 Stroke completed -- waiting for pressure to equalize ... @16:46:32.65 SS.reconfigure :exhale @16:46:32.70 SS.hold @16:46:41.15 Intake.select :close @16:46:41.30 SSV.dial :exhaust @16:46:42.49 Exhaust.select :open @16:46:42.61 SS.seek 0ml @16:46:51.53 Exhaust.select :close @16:46:51.65 SSV.dial CBV @16:46:51.68 CBV.dial SSV @16:46:52.44 Allowing 130s for 25.0ml of remaining 25.0ml @16:46:52.45 Intake.select :open @16:46:54.61 Intake @14.0psia, vacuum from 8.5 to 11.5psi @16:46:54.63 SS.reconfigure :inhaling 2.17ml/s @16:46:54.68 SS.seek 25ml @16:47:09.76 Stroke completed -- waiting for pressure to equalize ... @16:47:09.78 SS.reconfigure :exhale @16:47:09.83 SS.hold @16:47:19.62 Intake.select :close @16:47:19.77 SSV.dial :exhaust @16:47:20.53 Exhaust.select :open @16:47:20.65 SS.seek 0ml @16:47:29.94 Exhaust.select :close @16:47:30.05 Sampled 50.0ml @16:47:30.07 Drying puck before fixing for 4:00 @16:47:30.08 CBV.dial :waste2b @16:47:30.81 delay 1:00 @16:48:30.83 CSV.dial :air @16:48:31.56 CS.seek 10ml @16:48:55.31 Pumping wcr puck from tube 2 up to 35psi... @16:48:55.32 CBV.dial SSV @16:48:56.58 CBV.dial between CSV and waste2b @16:48:57.52 CSV.dial between air and CTV @16:48:58.27 CS.seek 7.401ml @16:49:05.09 CTV.dial CSV,avoiding: :air @16:49:05.12 CSV.dial CTV @16:49:05.95 CS.reconfigure :pressurize @16:49:06.00 CS.seek 0ml @16:49:21.57 CBV.dial :waste2b @16:49:21.90 CS.reconfigure :default @16:49:21.96 CS.seek 0ml @16:49:26.52 delay 1:00 @16:50:26.55 CTV.dial :air,avoiding: :intake @16:50:27.90 Fix #1 of 1 (with RNAlater) for 4:00 @16:50:27.92 CSV.dial CSR @16:50:28.35 Pulling 2ml of CSR.RNAlater @16:50:28.36 CSR.select :RNAlater @16:50:28.48 CS.seek 2ml @16:50:34.43 delay 5 seconds @16:50:39.45 CSR.select :air @16:50:39.57 CS.reconfigure :fast @16:50:39.62 CS.seek 10ml @16:50:58.62 Pumping wcr puck from tube 2 up to 35psi... @16:50:58.63 CBV.dial SSV @16:50:59.87 CBV.dial between CSV and waste2b @16:51:00.83 CSV.dial between air and CTV @16:51:01.44 CS.seek 7.297ml @16:51:08.46 CTV.dial CSV,avoiding: :air @16:51:08.49 CSV.dial CTV @16:51:09.88 CS.reconfigure :pressurize @16:51:09.94 CS.seek 0ml @16:51:26.07 delay 4:00 @16:55:26.10 CBV.dial :waste2b @16:55:26.43 CS.reconfigure :default @16:55:26.48 CS.seek 0ml @16:55:30.24 delay 1:00 @16:56:30.27 CTV.dial :air,avoiding: :intake @16:56:31.66 Storing wcr puck in Collection Clamp to tube 1 @16:56:31.84 SC.reconfigure :tight @16:56:31.89 SC.seek 1 @16:56:35.48 SC.configure :limp @16:56:35.69 Elbow.reconfigure :tight @16:56:35.74 Elbow.seek 1 @16:56:37.47 Forearm.reconfigure :tight @16:56:37.52 Forearm.seek 1 + 800 counts @16:56:38.58 SE.reconfigure :pick @16:56:38.64 SE.seek :beamBreak @16:56:41.46 SE.configure :default @16:56:41.51 SE.seek :place @16:56:42.02 Forearm.reconfigure :loose @16:56:42.07 Forearm.seek :retract @16:56:42.99 Elbow.seek CC @16:56:44.61 Elbow.reconfigure :loose @16:56:44.72 Forearm.seek CC @16:56:45.68 Hand.close @16:56:46.39 CC.seek :open @16:56:50.62 Forearm.seek :retract @16:56:51.69 Elbow.reconfigure :tight @16:56:51.74 Elbow.seek 1 @16:56:53.46 Elbow.reconfigure :limp @16:56:53.51 Forearm.seek 1 @16:56:54.70 Elbow.hold @16:56:54.77 Elbow.reconfigure :tight @16:56:54.82 Hand.open @16:56:55.63 Forearm.force -20 @16:56:55.68 Forearm.seek 1 + 310 counts @16:56:56.02 Forearm.reconfigure :tight @16:56:56.07 Forearm.seek 1 + 800 counts @16:56:56.44 SE.seek :down @16:56:57.57 Forearm.reconfigure :loose @16:56:57.62 Forearm.seek :retract @16:56:59.17 SE.stop @16:56:59.21
Starting finalPurge @16:56:59.22 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:56:59.84 Elbow.seek FlushPuck::Garage @16:57:01.80 Elbow.reconfigure :loose @16:57:01.90 Forearm.seek :garage @16:57:02.95 Hand.close @16:57:03.76 Forearm.seek :retract @16:57:04.81 Elbow.reconfigure :tight @16:57:04.86 Elbow.seek CC @16:57:07.69 Elbow.reconfigure :loose @16:57:07.79 Forearm.reconfigure :tight @16:57:07.84 Forearm.seek CC @16:57:09.17 CC.seek :closed @16:57:13.14 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.95 @16:57:13.21 Hand.open @16:57:13.96 Forearm.reconfigure :loose @16:57:14.00 Forearm.seek :retract @16:57:15.11 Flushing the Collection Valve::Manifold @16:57:15.24 CSV.dial CSR @16:57:15.67 Pulling 5ml of CSR.flush @16:57:15.68 CSR.select :flush @16:57:15.80 CS.seek 5ml @16:57:29.36 delay 5 seconds @16:57:34.38 CSR.select :air @16:57:34.54 CS.reconfigure :fast @16:57:34.59 CS.seek 7.5ml @16:57:41.26 CSV.dial :waste @16:57:42.53 CS.reconfigure :default @16:57:42.58 CS.seek 0ml @16:58:02.58 Flushing lower manifold's waste2b port @16:58:02.75 CSV.dial :flush @16:58:03.19 CS.reconfigure :fast @16:58:03.24 CS.seek 5ml @16:58:15.55 delay 5 seconds @16:58:20.56 CSV.dial :air @16:58:21.51 CS.seek 7ml @16:58:26.89 CSV.dial CTV @16:58:26.92
CTV.dial CSV @16:58:28.31 CBV.dial :waste2b @16:58:28.40 CS.seek 0ml @16:58:45.05 Disinfecting external Intake with kill @16:58:45.17 CSV.dial CSR @16:58:45.61 Pulling 8ml of CSR.kill @16:58:45.62 CSR.select :kill @16:58:45.74 CS.seek 8ml @16:59:04.95 delay 5 seconds @16:59:09.97 CSR.select :air @16:59:10.09 CS.seek 10ml @16:59:15.52 CSV.dial CBV @16:59:15.55
CBV.dial CSV @16:59:16.59 CTV.dial :intake @16:59:17.36 Intake.select :open @16:59:17.47 CS.seek 2ml @16:59:36.42 Intake.select :close @16:59:36.52 CTV.dial :air @16:59:37.29 CS.reconfigure :default @16:59:37.33 CS.seek 0ml @16:59:43.26 CSV.dial CSR @16:59:44.26 Pulling 5ml of CSR.flush @16:59:44.27 CSR.select :flush @16:59:44.39 CS.seek 5ml @16:59:58.05 delay 5 seconds @17:00:03.06 CSR.select :air @17:00:03.18 CS.seek 10ml @17:00:16.76 CSV.dial CBV @17:00:17.77 CS.seek 0ml @17:00:44.14 Garaging FlushPuck in Collection Clamp @17:00:44.21 Elbow.reconfigure :tight @17:00:44.25 Elbow.seek CC @17:00:44.37 Elbow.reconfigure :loose @17:00:44.47 Forearm.seek CC @17:00:45.41 Hand.close @17:00:46.14 CC.seek :open @17:00:50.22 Forearm.seek :retract @17:00:51.21 Elbow.reconfigure :tight @17:00:51.26 Elbow.seek :garage @17:00:54.19 Elbow.reconfigure :loose @17:00:54.29 Forearm.reconfigure :tight @17:00:54.34 Forearm.seek :garage @17:00:55.48 Hand.open @17:00:56.32 Forearm.reconfigure :loose @17:00:56.37 Forearm.seek :retract @17:00:57.39 Completed [initialPurge; standAloneWCR([nil,50], $da)] begun 16:41:01 @17:00:57.43 Asleep after completing standAloneWCR_4 assays @17:00:57.45 GoodNews.email "{1 => 1,\n 2 => 0}\nCTD data unavailable\nCan@17:00:13, 40% flow, 26.0C, 37% humidity, 14.9psia, 13.70V, 0.353A avg, 5.549Ah, 4.84W\nSampled 50.0ml",Subject: "Asleep after completing standAloneWCR_4 assays" @17:01:01.88 upsync + ESPwhich DA_WCR_110124.rb + fn=DA_WCR_110124.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_110124.rb + [ -f /home/esp/esp2/mission/DA_WCR_110124.rb ] + realpath /home/esp/esp2/mission/DA_WCR_110124.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_110124.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_110124.rb @13:59:50.41UTC29-Aug-25
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:59:53.20 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @13:59:56.96 Polling Can every 10:00 @13:59:56.97 Cleanly exited at 13:59:31UTC29-Aug-25 @13:59:57.03 Loading /home/esp/esp2local/mission/DA_WCR_110124.rb mission @13:59:57.08 Preparing to sleep until + 00:00:05 @13:59:57.10 GoodNews.email "Starting DA_WCR_110124 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@13:59:56, 40% flow, 24.3C, 41% humidity, 14.8psia, 13.72V, 0.271A avg, 0.027Ah, 3.72W",Subject: "DA_WCR_110124 begun" @13:59:57.16
Asleep til + 00:00:05 @13:59:57.18 Continuing at 14:00 @13:59:57.42 Gate.power :core,:OFF @13:59:57.43 Gate.power :raw,:OFF @13:59:57.44 Shutdown @14:00:02.05
Powering core bus /suscript/connectNetwork: No such file or directory @14:00:04.93 Errno::ECONNRESET in Qmail -- Connection reset by peer @14:00:04.95 Retry #1 of 10 after 10:00 @14:00:07.26
Gate.power :raw,:ON @14:00:08.11 CS.configure :default @14:00:08.50 PS.configure :default @14:00:08.58 SS.configure :default @14:00:08.63 CC.configure :default @14:00:08.68 PC.configure :default @14:00:08.74 Forearm.configure :loose @14:00:08.79 Elbow.configure :loose @14:00:08.86 SC.configure :loose @14:00:08.91 SE.configure :default @14:00:08.96 CH.configure :default @14:00:08.99 PH.configure :default @14:00:09.03 All dwarf controllers running firmware version 3.85 @14:00:09.14 Valve.reset @14:00:09.36 Intake.select :close @14:00:09.40 Exhaust.select :close @14:00:09.45 CTV.dial :air @14:00:09.55 PSV.dial :air @14:00:09.69 CSV.dial :waste1 @14:00:09.72
Forearm.home.seek :retract @14:00:09.85 SSV.dial :spit,avoiding: :exhaust @14:00:09.87 PS.home.seek 0ml @14:00:10.09 CS.reconfigure :fast @14:00:10.12 SS.home.seek 0ml @14:00:10.21 CS.home.seek 0ml @14:00:10.56 Hand.close @14:00:10.59
Elbow.reconfigure :loose @14:00:10.68 Elbow.jog 1500 @14:00:11.17 Elbow.reconfigure :loose @14:00:11.21 Elbow.home.seek :clear @14:00:15.63 SE.home.seek :down @14:00:16.31 SC.reconfigure :tight @14:00:16.37 SC.home.seek :initial @14:00:18.98 PS.stop @14:00:41.93
SC.configure :limp @14:00:41.99 Forearm.seek :retract @14:00:42.13 Elbow.seek :hammer @14:00:43.16 Forearm.reconfigure :tight @14:00:43.21 Forearm.seek retracted @14:00:43.47 PC.home.seek :open @14:00:43.48
CC.home.seek :open @14:00:46.74 Phase [initialPurge; da [50, 50] #da and wsr are run simultanelously] @14:00:46.75 Starting initialPurge @14:00:46.76 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:00:46.83 Hand.open @14:00:47.74 Elbow.reconfigure :tight @14:00:47.79 Elbow.seek FlushPuck::Garage @14:00:49.60 Elbow.reconfigure :loose @14:00:49.70 Forearm.reconfigure :loose @14:00:49.74 Forearm.seek :garage @14:00:50.70 Hand.close @14:00:51.62 Forearm.seek :retract @14:00:52.79 Elbow.reconfigure :tight @14:00:52.84 Elbow.seek CC @14:00:55.70 Elbow.reconfigure :loose @14:00:55.81 Forearm.reconfigure :tight @14:00:55.85 Forearm.seek CC @14:00:57.07 CC.seek :closed @14:01:01.07 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.07 @14:01:01.14 Hand.open @14:01:01.93 Forearm.reconfigure :loose @14:01:01.97 Forearm.seek :retract @14:01:03.05 Flushing the Collection Valve::Manifold @14:01:03.18 CSV.dial CSR @14:01:04.57 Pulling 5ml of CSR.flush @14:01:04.58 CSR.select :flush @14:01:04.69 CS.reconfigure :default @14:01:04.74 CS.seek 5ml @14:01:18.36 delay 5 seconds @14:01:23.38 CSR.select :air @14:01:23.55 CS.reconfigure :fast @14:01:23.60 CS.seek 7.5ml @14:01:30.40 CSV.dial :waste @14:01:31.67 CS.reconfigure :default @14:01:31.72 CS.seek 0ml @14:01:51.71 Purging external Intake @14:01:51.83 CSV.dial :flush @14:01:52.29 CS.reconfigure :fast @14:01:52.34 CS.seek 10ml @14:02:16.43 delay 5 seconds @14:02:21.45 CSV.dial CBV @14:02:21.48
CBV.dial CSV @14:02:22.35 CTV.dial :intake @14:02:23.11 Intake.select :open @14:02:23.22 CS.seek 0ml @14:02:46.80 Intake.select :close @14:02:46.91 CTV.dial :air @14:02:47.69 Flushing upper manifold @14:02:47.82 CSV.dial :flush @14:02:48.53 CS.seek 5ml @14:03:00.80 delay 5 seconds @14:03:05.82 CSV.dial :air @14:03:06.32 CS.seek 7ml @14:03:11.77 CSV.dial CBV @14:03:11.81
CBV.dial CSV @14:03:12.80 CTV.dial :air @14:03:12.88 CS.seek 0ml @14:03:29.61 delay 7 seconds @14:03:36.64 CS.seek 3ml @14:03:44.42 CSV.dial :waste @14:03:44.88 CS.seek 0ml @14:03:52.69 CSV.dial :flush @14:03:53.14 CS.seek 3ml @14:04:00.83 delay 5 seconds @14:04:05.85 CSV.dial :air @14:04:06.36 CS.seek 5ml @14:04:11.86 CSV.dial :air @14:04:12.04 CS.seek 0ml @14:04:24.24 delay 7 seconds @14:04:31.26 CS.seek 3ml @14:04:39.05 CSV.dial :waste @14:04:39.77 CS.seek 0ml @14:04:47.52 Garaging FlushPuck in Collection Clamp @14:04:47.59 Elbow.reconfigure :tight @14:04:47.64 Elbow.seek CC @14:04:47.75 Elbow.reconfigure :loose @14:04:47.85 Forearm.seek CC @14:04:48.83 Hand.close @14:04:49.62 CC.seek :open @14:04:53.73 Forearm.seek :retract @14:04:54.88 Elbow.reconfigure :tight @14:04:54.93 Elbow.seek :garage @14:04:57.81 Elbow.reconfigure :loose @14:04:57.91 Forearm.reconfigure :tight @14:04:57.96 Forearm.seek :garage @14:04:59.08 Hand.open @14:04:59.99 Forearm.reconfigure :loose @14:05:00.04 Forearm.seek :retract @14:05:01.09 da sampling at most 50ml, wcr at most 50ml @14:05:01.12 Loading sh1 puck from tube 2 into Collection Clamp @14:05:01.35 SC.reconfigure :tight @14:05:01.40 SC.seek 2 @14:05:08.26 SC.configure :limp @14:05:08.48 Elbow.reconfigure :tight @14:05:08.53 Elbow.seek 2 @14:05:10.45 Forearm.reconfigure :tight @14:05:10.50 Forearm.seek 2 + 800 counts @14:05:11.55 SE.reconfigure :pick @14:05:11.61 SE.seek :beamBreak @14:05:14.40 SE.configure :default @14:05:14.57 Elbow.seek 2 @14:05:14.76 Forearm.seek 2 + 300 counts @14:05:14.92 Forearm.reconfigure :loose @14:05:14.97 Hand.close @14:05:15.75 SE.seek :down @14:05:15.95
Forearm.seek :retract @14:05:17.01 Elbow.seek CC @14:05:18.29 SE.stop @14:05:18.59
Elbow.reconfigure :loose @14:05:18.70 Forearm.reconfigure :tight @14:05:18.74 Forearm.seek CC @14:05:20.14 CC.seek :closed @14:05:24.57 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.01 @14:05:24.58 Hand.open @14:05:25.42 Forearm.reconfigure :loose @14:05:25.47 Forearm.seek :retract @14:05:26.43 collectSample 50ml w/bubble point of 24psi @14:05:26.44 Priming 7ml @14:05:26.45 Intake.select :close @14:05:26.46 Exhaust.select :close @14:05:26.47 SS.hold @14:05:26.69 Calibrating pressure and temperature sensors @14:05:26.72 CTV.dial :air @14:05:26.80 SSV.dial :spit,avoiding: :exhaust @14:05:26.82 SS.reconfigure :fast @14:05:26.87 SS.seek 5ml @14:05:29.59 SSV.dial CBV @14:05:29.63
CBV.dial SSV @14:05:30.97 delay 3.8 seconds @14:05:34.89 Sensor limits: Intake max 75.2psia; Outlet max 104.2psia @14:05:34.90 SSV.dial :spit @14:05:36.24 SS.seek 0ml @14:05:38.81 SSV.dial CBV @14:05:40.14 CTV.dial :intake @14:05:40.91 SSV.dial CBV @14:05:40.95
CBV.dial SSV @14:05:41.17 Allowing 40s for 7.0ml of remaining 7.0ml @14:05:41.18 Intake.select :open @14:05:43.35 Intake @13.9psia, vacuum from 8.4 to 11.4psi @14:05:43.36 SS.reconfigure :inhaling 2.17ml/s @14:05:43.41 SS.seek 7ml @14:05:50.07 Stroke completed -- waiting for pressure to equalize ... @14:05:50.08 SS.reconfigure :exhale @14:05:50.13 SS.hold @14:05:50.47 Intake.select :close @14:05:50.63 SSV.dial :exhaust @14:05:51.38 Exhaust.select :open @14:05:51.49 SS.seek 0ml @14:05:55.45 Exhaust.select :close @14:05:55.56 Sampling 50.0ml @14:05:55.57 Intake.select :close @14:05:55.58 Exhaust.select :close @14:05:55.60 SS.reconfigure :default @14:05:55.64 SS.hold @14:05:55.85 CTV.dial :intake @14:05:55.87 SSV.dial CBV @14:05:55.90
CBV.dial SSV @14:05:56.67 Allowing 130s for 25.0ml of remaining 50.0ml @14:05:56.68 Intake.select :open @14:05:58.84 Intake @13.9psia, vacuum from 8.4 to 11.4psi @14:05:58.86 SS.reconfigure :inhaling 2.17ml/s @14:05:58.91 SS.seek 25ml @14:06:14.57 Stroke completed -- waiting for pressure to equalize ... @14:06:14.58 SS.reconfigure :exhale @14:06:14.63 SS.hold @14:06:19.64 Intake.select :close @14:06:19.79 SSV.dial :exhaust @14:06:20.56 Exhaust.select :open @14:06:20.67 SS.seek 0ml @14:06:29.62 Exhaust.select :close @14:06:29.73 SSV.dial CBV @14:06:29.75
CBV.dial SSV @14:06:30.54 Allowing 130s for 25.0ml of remaining 25.0ml @14:06:30.55 Intake.select :open @14:06:32.72 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:06:32.74 SS.reconfigure :inhaling 2.17ml/s @14:06:32.79 SS.seek 25ml @14:06:48.41 Stroke completed -- waiting for pressure to equalize ... @14:06:48.42 SS.reconfigure :exhale @14:06:48.47 SS.hold @14:06:54.10 Intake.select :close @14:06:54.26 SSV.dial :exhaust @14:06:55.02 Exhaust.select :open @14:06:55.13 SS.seek 0ml @14:07:04.04 Exhaust.select :close @14:07:04.15 Sampled 50.0ml @14:07:04.16 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:07:04.17 Intake.select :close @14:07:04.24 CSV.dial :waste @14:07:04.36 SSV.dial CBV,avoiding: :exhaust @14:07:04.38
CBV.dial SSV @14:07:04.52 CS.seek 0ml @14:07:04.59 CSV.dial :air @14:07:05.30 CS.seek 10ml @14:07:06.41
CTV.dial :air @14:07:07.17 delay 15 seconds @14:07:22.19 SSV.dial :spit,avoiding: :exhaust @14:07:23.54 CTV.dial CSV,avoiding: :intake @14:07:23.58
SS.seek 0ml @14:07:23.71 SSV.dial CBV,avoiding: :exhaust @14:07:29.67 SSV.dial between CBV and plug @14:07:30.18 CBV.dial between SSV and waste2 @14:07:30.60 CTV.dial CSV,avoiding: :air @14:07:30.62
CSV.dial CTV @14:07:31.10 CS.reconfigure :pressurize @14:07:31.15 CS.seek 0ml @14:07:52.24 BubblePoint: Intake @24.3psia, Outlet @0.1psia @14:07:52.25 CBV.dial SSV @14:07:52.60 SS.reconfigure :default @14:07:52.65 SS.seek 10ml @14:07:58.57 delay 15 seconds @14:08:13.70 Evacuated: Intake @23.7psia, Outlet @23.7psia @14:08:13.71 SSV.dial :spit,avoiding: :exhaust @14:08:14.76 SS.reconfigure :exhale @14:08:14.81 SS.seek 0ml @14:08:20.14 SS.reconfigure :fast @14:08:20.19 SS.seek 5ml @14:08:23.23 SSV.dial CBV,avoiding: :exhaust @14:08:23.28
CBV.dial SSV @14:08:24.60 CTV.dial :air,avoiding: :intake @14:08:25.97 CSV.dial CTV @14:08:26.02
SS.seek 10ml @14:08:27.18 CSV.dial :air @14:08:27.61 CS.reconfigure :fast @14:08:27.66 CS.seek 0ml @14:08:28.71
SSV.dial :spit,avoiding: :exhaust @14:08:30.04 SS.reconfigure :exhale @14:08:30.09 SS.seek 0ml @14:08:32.92 CS.stop @14:08:35.50
SS.reconfigure :fast @14:08:35.56 SS.seek 5ml @14:08:38.51 SSV.dial CBV,avoiding: :exhaust @14:08:38.54
CBV.dial SSV @14:08:40.91 SSV.dial :spit,avoiding: :exhaust @14:08:42.26 SS.reconfigure :default @14:08:42.31 SS.seek 0ml @14:08:45.41 SS.stop @14:08:45.45 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:08:45.46 Intake.select :close @14:08:45.53 CSV.dial :waste @14:08:45.65 SSV.dial CBV,avoiding: :exhaust @14:08:45.67
CBV.dial SSV @14:08:46.30 CS.seek 0ml @14:08:46.37 CSV.dial :air @14:08:47.10 CS.seek 10ml @14:08:47.12
CTV.dial :air @14:08:47.67 delay 15 seconds @14:09:02.70 SSV.dial :spit,avoiding: :exhaust @14:09:04.04 CTV.dial CSV,avoiding: :intake @14:09:04.08
SS.reconfigure :exhale @14:09:04.18 SS.seek 0ml @14:09:04.63 SSV.dial CBV,avoiding: :exhaust @14:09:11.42 SSV.dial between CBV and plug @14:09:11.93 CBV.dial between SSV and waste2 @14:09:12.33 CTV.dial CSV,avoiding: :air @14:09:12.36
CSV.dial CTV @14:09:12.83 CS.reconfigure :pressurize @14:09:12.88 CS.seek 0ml @14:09:36.68 BubblePoint: Intake @24.2psia, Outlet @0.3psia @14:09:36.69 CBV.dial SSV @14:09:37.03 SS.reconfigure :default @14:09:37.08 SS.seek 10ml @14:09:42.99 delay 15 seconds @14:09:58.12 Evacuated: Intake @23.8psia, Outlet @10.6psia @14:09:58.13 SSV.dial :spit,avoiding: :exhaust @14:09:59.20 SS.reconfigure :exhale @14:09:59.25 SS.seek 0ml @14:10:04.33 SS.reconfigure :fast @14:10:04.38 SS.seek 5ml @14:10:07.21 SSV.dial CBV,avoiding: :exhaust @14:10:07.24
CBV.dial SSV @14:10:08.21 GoodNews.email "Then, in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_110124.rb:14\n{2 => 4}\nCTD data unavailable\nCan@13:59:57, 40% flow, 24.3C, 41% humidity, 14.8psia, 13.72V, 0.265A avg, 0.027Ah, 3.64W",Subject: "Asleep until 14:00" @14:10:08.65
CTV.dial :air,avoiding: :intake @14:10:10.09 CSV.dial CTV @14:10:10.13
SS.seek 10ml @14:10:10.52 upsync @14:10:11.35 CSV.dial :air @14:10:11.87 CS.reconfigure :fast @14:10:11.91 Notice.email "At 14:00:46 began in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_110124.rb:14\n{2 => 4}\nCTD data unavailable\nCan@14:00:46, 40% flow, 24.4C, 41% humidity, 14.8psia, 13.72V, 0.208A avg, 0.032Ah, 2.86W",Subject: "Started DA_WCR_110124 phase initialPurge; da [50, 50] #da and wsr are run simultanelously" @14:10:12.67 CS.seek 0ml @14:10:12.82
SSV.dial :spit,avoiding: :exhaust @14:10:13.66 upsync @14:10:14.16
SS.reconfigure :exhale @14:10:14.23 SS.seek 0ml @14:10:15.54 CS.stop @14:10:16.74 DAfans.email "Started da in tube 2 at 14:05:01UTC29-Aug-25\n{2 => 4}\nCTD data unavailable\nCan@14:00:46, 40% flow, 24.4C, 41% humidity, 14.8psia, 13.72V, 0.208A avg, 0.032Ah, 2.86W",Subject: "da sampling at most 50ml, wcr at most 50ml" @14:10:18.37 upsync @14:10:19.37
SS.reconfigure :fast @14:10:19.43 SS.seek 5ml /suscript/connectNetwork: No such file or directory @14:10:20.71 Errno::ECONNRESET in Qmail -- Connection reset by peer @14:10:20.73 Retry #1 of 10 after 10:00 @14:10:22.37 SSV.dial CBV,avoiding: :exhaust @14:10:22.40
CBV.dial SSV @14:10:24.77 SSV.dial :spit,avoiding: :exhaust @14:10:26.12 SS.reconfigure :default @14:10:26.17 SS.seek 0ml @14:10:29.24 SS.stop @14:10:29.28 Lysing: Adding 2.0ml AqMeOH @14:10:29.29 CBV.dial between CSV and waste1,avoiding: :DBV @14:10:30.80 CTV.dial CSV @14:10:32.14 CSV.dial CSR @14:10:32.92 Pulling 0.999ml of CSR.AqMeOH CSR.select :AqMeOH @14:10:33.04 CS.reconfigure :default @14:10:33.09 CS.seek 1ml @14:10:36.54 delay 5 seconds @14:10:41.56 CSR.select :air @14:10:41.73 CS.seek 6ml @14:10:55.42 delay 2 seconds @14:10:57.44 CSV.dial :waste2 @14:10:57.88 CS.reconfigure :fast @14:10:57.93 CS.seek 0ml @14:11:12.36 CSV.dial CSR @14:11:12.81 Pulling 2ml of CSR.AqMeOH @14:11:12.82 CSR.select :AqMeOH @14:11:12.94 CS.reconfigure :default @14:11:12.99 CS.seek 2ml @14:11:18.98 delay 5 seconds @14:11:24.00 CSR.select :air @14:11:24.17 CS.seek 5ml @14:11:32.67 delay 2 seconds @14:11:34.68 CSV.dial CTV @14:11:35.57 CS.seek 1ml @14:11:46.69 Before lysing Collection Puck Heater at 22.8C/14.8psia @14:11:46.71 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @14:11:46.72 CH.seek 70C,8:00 @14:13:52.19 CH.hold 10:00,70C @14:13:52.39 Begun lysing Collection Puck Heater at 68.1C/21.1psia /suscript/connectNetwork: No such file or directory @14:20:25.41 Errno::ECONNRESET in Qmail -- Connection reset by peer @14:20:25.43 Retry #2 of 10 after 10:00 @14:23:52.41
Ended lysing Collection Puck Heater at 70C/14.0psia @14:23:52.43 CS.seek 0ml @14:23:55.86 CTV.dial between spare and air,avoiding: :intake @14:23:56.92 CSV.dial :air @14:23:57.38 CS.seek 5ml @14:24:11.19 CSV.dial CTV @14:24:11.64 delay 2 seconds @14:24:13.66 CSV.dial :air @14:24:14.11 delay 2 seconds @14:24:16.13 CSV.dial CTV @14:24:16.58 delay 2 seconds @14:24:18.60 CSV.dial :air @14:24:19.05 delay 2 seconds @14:24:21.07 CS.seek 0ml @14:24:34.81 Puck @22.7psia after final lysis pressurization @14:24:34.82 Recovering Lysate into Collection syringe @14:24:34.83 CSV.dial CBV @14:24:35.83 CBV.dial CSV @14:24:36.22 CS.seek 5ml @14:24:49.91 delay 5 seconds @14:24:54.93 CTV.dial :air @14:24:55.47 CS.seek 7ml @14:25:01.59 delay 5 seconds @14:25:06.61 CSV.dial :air2 @14:25:07.07 CS.seek 10ml @14:25:15.63 CSV.dial between air and CTV,avoiding: CBV @14:25:16.77 Lysing Complete @14:25:16.84 Storing sh1 puck in Collection Clamp to tube 1 @14:25:17.03 SC.reconfigure :tight @14:25:17.08 SC.seek 1 @14:25:20.61 SC.configure :limp @14:25:20.84 Elbow.reconfigure :tight @14:25:20.89 Elbow.seek 1 @14:25:23.06 Forearm.reconfigure :tight @14:25:23.11 Forearm.seek 1 + 800 counts @14:25:24.15 SE.reconfigure :pick @14:25:24.21 SE.seek :beamBreak @14:25:27.04 SE.configure :default @14:25:27.09 SE.seek :place @14:25:27.62 Forearm.reconfigure :loose @14:25:27.66 Forearm.seek :retract @14:25:28.62 Elbow.seek CC @14:25:30.29 Elbow.reconfigure :loose @14:25:30.39 Forearm.seek CC @14:25:31.36 Hand.close @14:25:32.09 CC.seek :open @14:25:36.30 Forearm.seek :retract @14:25:37.42 Elbow.reconfigure :tight @14:25:37.47 Elbow.seek 1 @14:25:39.19 Elbow.reconfigure :limp @14:25:39.25 Forearm.seek 1 @14:25:40.39 Elbow.hold @14:25:40.46 Elbow.reconfigure :tight @14:25:40.51 Hand.open @14:25:41.33 Forearm.force -20 @14:25:41.38 Forearm.seek 1 + 309 counts @14:25:41.74 Forearm.reconfigure :tight @14:25:41.79 Forearm.seek 1 + 800 counts @14:25:42.20 SE.seek :down @14:25:43.34
Forearm.reconfigure :loose @14:25:43.39 Forearm.seek :retract @14:25:44.97 SE.stop @14:25:45.01
Tranferring DA @14:25:45.02 Beginning samplePassC @14:25:45.04 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:25:45.67 Elbow.seek FlushPuck::Garage @14:25:47.46 Elbow.reconfigure :loose @14:25:47.56 Forearm.seek :garage @14:25:48.62 Hand.close @14:25:49.46 Forearm.seek :retract @14:25:50.45 Elbow.reconfigure :tight @14:25:50.50 Elbow.seek CC @14:25:53.38 Elbow.reconfigure :loose @14:25:53.48 Forearm.reconfigure :tight @14:25:53.53 Forearm.seek CC @14:25:54.74 CC.seek :closed @14:25:58.75 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 2.02 @14:25:58.82 Hand.open @14:25:59.65 Forearm.reconfigure :loose @14:25:59.70 Forearm.seek :retract @14:26:00.70 PSV.dial PSR @14:26:02.05 Pulling 1.5ml of PSR.flush @14:26:02.06 PSR.select :flush @14:26:02.18 PS.seek 1.5ml @14:26:18.30 delay 5 seconds @14:26:23.31 PSV.dial :waste1 @14:26:23.88 PS.seek 0ml @14:26:39.98 PSV.dial PSR @14:26:40.42 PSR.select :air @14:26:40.54 PS.seek 2.5ml @14:27:06.96 PRV.dial CBV,avoiding: :ab2 @14:27:06.98
CBV.dial PRV @14:27:08.16 PSV.dial :mixloop @14:27:09.27 CTV.dial :air,avoiding: :intake @14:27:09.34 PTV.dial :PRVmixing @14:27:10.10 PS.seek 0ml @14:27:36.54 PSV.dial :air @14:27:37.00 PS.seek 2.5ml @14:28:03.42 PSV.dial :mixloop @14:28:04.00 PS.seek 0ml @14:28:30.32 PSV.dial :air @14:28:30.78 PS.seek 2.5ml @14:28:57.10 PSV.dial :mixloop @14:28:57.58 PS.seek 0ml @14:29:23.95 Garaging FlushPuck in Collection Clamp @14:29:24.02 Elbow.reconfigure :tight @14:29:24.07 Elbow.seek CC @14:29:24.36 Elbow.reconfigure :loose @14:29:24.47 Forearm.seek CC @14:29:25.45 Hand.close @14:29:26.22 CC.seek :open @14:29:30.31 Forearm.seek :retract @14:29:31.37 Elbow.reconfigure :tight @14:29:31.42 Elbow.seek :garage @14:29:34.17 Elbow.reconfigure :loose @14:29:34.27 Forearm.reconfigure :tight @14:29:34.32 Forearm.seek :garage @14:29:35.51 Hand.open @14:29:36.27 Forearm.reconfigure :loose @14:29:36.32 Forearm.seek :retract @14:29:37.40 PSV.dial :mixloop @14:29:37.57 PTV.dial :PRVmixing @14:29:37.65 PRV.dial CBV @14:29:37.68
CBV.dial PRV @14:29:37.82 CTV.dial :air,avoiding: :intake @14:29:37.91 Loading lyfil puck from tube 2 into Collection Clamp @14:29:38.14 SC.reconfigure :tight @14:29:38.19 SC.seek 2 @14:29:42.47 SC.configure :limp @14:29:42.72 Elbow.reconfigure :tight @14:29:42.77 Elbow.seek 2 @14:29:44.66 Forearm.reconfigure :tight @14:29:44.71 Forearm.seek 2 + 800 counts @14:29:45.82 SE.reconfigure :pick @14:29:45.88 SE.seek :beamBreak @14:29:48.71 SE.configure :default @14:29:48.87 Elbow.seek 2 @14:29:49.06 Forearm.seek 2 + 300 counts @14:29:49.40 Forearm.reconfigure :loose @14:29:49.45 Hand.close @14:29:50.23 SE.seek :down @14:29:50.43
Forearm.seek :retract @14:29:51.39 Elbow.seek CC @14:29:52.86 SE.stop @14:29:52.97
Elbow.reconfigure :loose @14:29:53.08 Forearm.reconfigure :tight @14:29:53.13 Forearm.seek CC @14:29:54.41 CC.seek :closed @14:29:58.42 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.98 @14:29:58.43 Hand.open @14:29:59.35 Forearm.reconfigure :loose @14:29:59.40 Forearm.seek :retract @14:30:00.42 CTV.dial CSV,avoiding: :intake @14:30:01.78 CSV.dial CTV @14:30:02.10 PS.seek 2.5ml @14:30:28.54 CS.seek 5ml /suscript/connectNetwork: No such file or directory @14:30:29.68 Errno::ECONNRESET in Qmail -- Connection reset by peer @14:30:29.71 Retry #3 of 10 after 10:00 @14:30:42.22
delay 8 seconds @14:30:50.24 PSV.dial :air @14:30:50.70 delay 2 seconds @14:30:52.72 PSV.dial :waste2 @14:30:53.46 PS.seek 0ml @14:31:19.79 CBV.dial between PRV and waste1 @14:31:20.12 CSV.dial :air @14:31:20.58 CS.seek 10ml @14:31:34.25 CSV.dial CTV @14:31:34.71 CS.seek 0ml @14:32:01.06 CBV.dial :waste1 @14:32:01.39 delay 5 seconds @14:32:06.41 CTV.dial :air,avoiding: :intake @14:32:07.84 Storing lyfil puck in Collection Clamp to tube 1 @14:32:08.02 SC.reconfigure :tight @14:32:08.07 SC.seek 1 @14:32:11.63 SC.configure :limp @14:32:11.85 Elbow.reconfigure :tight @14:32:11.90 Elbow.seek 1 @14:32:13.61 Forearm.reconfigure :tight @14:32:13.66 Forearm.seek 1 + 800 counts @14:32:14.90 SE.reconfigure :pick @14:32:15.42 SE.seek :beamBreak @14:32:18.27 SE.configure :default @14:32:18.44 Elbow.reconfigure :limp @14:32:18.49 Forearm.reconfigure :loose @14:32:18.54 Forearm.seek 1 @14:32:18.91 Elbow.hold @14:32:18.98 Elbow.reconfigure :tight @14:32:19.08 SE.seek 0.1 Pucks below Top Plate @14:32:19.54 Forearm.force -20 @14:32:19.58 Forearm.seek 1 + 310 counts @14:32:20.02 Forearm.reconfigure :tight @14:32:20.07 Forearm.seek 1 + 800 counts @14:32:20.34 SE.seek 1.1 Pucks below Top Plate @14:32:20.88 Forearm.reconfigure :loose @14:32:20.93 Forearm.seek :retract @14:32:21.85 Elbow.seek CC @14:32:23.54 Elbow.reconfigure :loose @14:32:23.64 Forearm.seek CC @14:32:24.66 Hand.close @14:32:25.46 CC.seek :open @14:32:30.19 Forearm.seek :retract @14:32:31.23 Elbow.reconfigure :tight @14:32:31.28 Elbow.seek 1 @14:32:33.02 Elbow.reconfigure :limp @14:32:33.07 Forearm.seek 1 @14:32:34.24 Elbow.hold @14:32:34.31 Elbow.reconfigure :tight @14:32:34.74 Hand.open @14:32:35.71 Forearm.force -20 @14:32:35.75 Forearm.seek 1 + 318 counts @14:32:36.11 Forearm.reconfigure :tight @14:32:36.17 Forearm.seek 1 + 800 counts @14:32:36.51 SE.seek :down @14:32:37.64
Forearm.reconfigure :loose @14:32:37.69 Forearm.seek :retract @14:32:39.09 SE.stop @14:32:39.13
PSV.dial :air @14:32:39.88 PS.seek 2.5ml @14:33:06.20 delay 1 second @14:33:07.21 PSV.dial :mixloop @14:33:07.67 delay 1 second @14:33:08.68 PSV.dial :air @14:33:09.15 delay 1 second @14:33:10.17 PSV.dial :mixloop @14:33:10.64 delay 1 second @14:33:11.65 PSV.dial :air @14:33:12.12 delay 1 second @14:33:13.14 PS.seek 0ml @14:33:39.51 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:33:39.70 Elbow.seek FlushPuck::Garage @14:33:41.47 Elbow.reconfigure :loose @14:33:41.57 Forearm.seek :garage @14:33:42.64 Hand.close @14:33:43.45 Forearm.seek :retract @14:33:44.48 Elbow.reconfigure :tight @14:33:44.53 Elbow.seek PC @14:33:45.83 Elbow.reconfigure :loose @14:33:45.93 Forearm.reconfigure :tight @14:33:45.98 Forearm.seek PC @14:33:47.83 PC.seek :closed @14:33:52.27 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.58 @14:33:52.33 Hand.open @14:33:53.15 Forearm.reconfigure :loose @14:33:53.19 Forearm.seek :retract @14:33:54.19 PRV.dial :air @14:33:54.67 PSV.dial :mixloop @14:33:55.20 PS.seek 0.3ml @14:33:59.16 PSV.dial PSR @14:34:00.20 Pulling 0.4ml of PSR.air @14:34:00.21 PSR.select :air @14:34:00.23 PS.seek 0.7ml @14:34:05.10 delay 3 seconds @14:34:08.12 PSR.select :air @14:34:08.14 PS.seek 2.5ml @14:34:27.29 delay 3 seconds @14:34:30.31 PSV.dial :mixloop @14:34:31.35 PTV.dial :mixing @14:34:32.09 PBV.dial :waste2 @14:34:33.09 PS.seek 0ml @14:34:59.51 Garaging FlushPuck in Processing Clamp @14:34:59.58 Elbow.reconfigure :tight @14:34:59.63 Elbow.seek PC @14:34:59.75 Elbow.reconfigure :loose @14:34:59.86 Forearm.seek PC @14:35:00.94 Hand.close @14:35:01.71 PC.seek :open @14:35:06.22 Forearm.seek :retract @14:35:07.22 Elbow.reconfigure :tight @14:35:07.27 Elbow.seek :garage @14:35:08.61 Elbow.reconfigure :loose @14:35:09.16 Forearm.reconfigure :tight @14:35:09.21 Forearm.seek :garage @14:35:10.36 Hand.open @14:35:11.15 Forearm.reconfigure :loose @14:35:11.20 Forearm.seek :retract @14:35:12.29 Loading da puck from tube 2 into Processing Clamp @14:35:12.52 SC.reconfigure :tight @14:35:12.57 SC.seek 2 @14:35:16.23 SC.configure :limp @14:35:16.44 Elbow.reconfigure :tight @14:35:16.49 Elbow.seek 2 @14:35:18.39 Forearm.reconfigure :tight @14:35:18.44 Forearm.seek 2 + 800 counts @14:35:19.49 SE.reconfigure :pick @14:35:19.55 SE.seek :beamBreak @14:35:22.37 SE.configure :default @14:35:22.54 Elbow.seek 2 @14:35:22.81 Forearm.seek 2 + 300 counts @14:35:22.99 Forearm.reconfigure :loose @14:35:23.03 Hand.close @14:35:23.85 SE.seek :down @14:35:24.05
Forearm.seek :retract @14:35:25.15 Elbow.seek PC @14:35:26.57 SE.stop @14:35:27.69
Elbow.reconfigure :loose @14:35:27.80 Forearm.reconfigure :tight @14:35:27.85 Forearm.seek PC @14:35:29.68 PC.seek :closed @14:35:34.13 Processing Clamp onSpring/offSpring for 12/26 samples, on/off torque 1.55 @14:35:34.14 Hand.open @14:35:34.89 Forearm.reconfigure :loose @14:35:34.94 Forearm.seek :retract @14:35:36.04 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @14:35:36.06 PH.seek 28C,5:00 @14:36:02.54 PH.hold 2:00:00,28C @14:36:02.60 delay 1:30 @14:37:32.63 PSR.select :air @14:37:32.65 PSV.dial PSR @14:37:33.80 Pulling 0.5ml of PSR.wash @14:37:33.81 PSR.select :wash @14:37:33.93 PS.seek 0.5ml @14:37:39.91 delay 5 seconds @14:37:44.93 PSR.select :air @14:37:45.05 PS.seek 2ml @14:38:01.25 PTV.dial :puck @14:38:01.99 PSV.dial PTV @14:38:02.72 PBV.dial :waste1 @14:38:04.16 PS.seek 1.5ml @14:38:10.51 delay 3 seconds @14:38:13.52 PBV.dial between PSV and waste1 @14:38:13.90 PS.seek 0ml @14:38:30.01 delay 5:00 @14:40:34.60 upsync @14:43:30.03
recoverPSRreagent08 @14:43:30.04 PTV.dial :puck @14:43:30.13 PBV.dial :waste1 @14:43:30.52 delay 30 seconds @14:44:00.54 PBV.dial between PSV and waste1 @14:44:00.94 PSR.select :air @14:44:00.96 PSV.dial PSR @14:44:01.69 PS.reconfigure :fast @14:44:01.74 PS.seek 2.5ml @14:44:25.28 PSV.dial PTV @14:44:26.03 delay 5 seconds @14:44:31.05 PSV.dial PSR @14:44:31.80 delay 5 seconds @14:44:36.82 PSV.dial PTV @14:44:37.58 delay 5 seconds @14:44:42.60 PSV.dial :air @14:44:43.37 PS.seek 0ml @14:45:06.75 PTV.dial :PRVmixing,via: PRV @14:45:08.05 PSV.dial :mixloop @14:45:08.52 PRV.dial :ab1 @14:45:09.27 PS.reconfigure :slow @14:45:09.32 PS.seek 0.15ml @14:45:12.88 delay 2 seconds @14:45:14.90 CBV.dial PRV @14:45:14.92
PRV.dial CBV @14:45:15.51 PS.seek 0.35ml @14:45:20.19 delay 2 seconds @14:45:22.21 CBV.dial between PRV and PSV @14:45:22.53 PRV.dial :air @14:45:22.99 PS.seek 0.9ml @14:45:34.69 PRV.dial :waste2 @14:45:35.43 PS.seek 0.5ml @14:45:44.06 addPMLReagent08 @14:45:44.07 PSV.dial :air @14:45:44.55 PS.reconfigure :default @14:45:44.60 PS.seek 2ml @14:46:00.72 PSV.dial :PML @14:46:01.19 PTV.dial :mixing @14:46:02.37 PBV.dial :waste1 @14:46:02.83 PS.seek 1.85ml @14:46:05.19 PBV.dial between PSV and waste1 @14:46:05.58 PS.seek 0ml @14:46:25.34 delay 2 seconds @14:46:27.36 Beginning samplePassC @14:46:27.38 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:46:27.57 Elbow.reconfigure :tight @14:46:27.61 Elbow.seek FlushPuck::Garage @14:46:28.99 Elbow.reconfigure :loose @14:46:29.09 Forearm.seek :garage @14:46:30.10 Hand.close @14:46:30.95 Forearm.seek :retract @14:46:31.96 Elbow.reconfigure :tight @14:46:32.01 Elbow.seek CC @14:46:34.84 Elbow.reconfigure :loose @14:46:34.95 Forearm.reconfigure :tight @14:46:35.00 Forearm.seek CC @14:46:36.26 CC.seek :closed @14:46:40.24 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.98 @14:46:40.31 Hand.open @14:46:41.14 Forearm.reconfigure :loose @14:46:41.18 Forearm.seek :retract @14:46:42.30 PSV.dial PSR @14:46:43.33 Pulling 1.5ml of PSR.flush @14:46:43.34 PSR.select :flush @14:46:43.46 PS.seek 1.5ml @14:47:00.00 delay 5 seconds @14:47:05.02 PSV.dial :waste1 @14:47:05.47 PS.seek 0ml @14:47:21.65 PSV.dial PSR @14:47:22.11 PSR.select :air @14:47:22.23 PS.seek 2.5ml @14:47:48.69 PRV.dial CBV,avoiding: :ab2 @14:47:48.71
CBV.dial PRV @14:47:50.36 PSV.dial :mixloop @14:47:51.39 CTV.dial :air,avoiding: :intake @14:47:51.46 PTV.dial :PRVmixing @14:47:52.22 PS.seek 0ml @14:48:18.71 PSV.dial :air @14:48:19.17 PS.seek 2.5ml @14:48:45.56 PSV.dial :mixloop @14:48:46.03 PS.seek 0ml @14:49:12.56 PSV.dial :air @14:49:13.03 PS.seek 2.5ml @14:49:39.37 PSV.dial :mixloop @14:49:39.85 PS.seek 0ml @14:50:06.22 Garaging FlushPuck in Collection Clamp @14:50:06.29 Elbow.reconfigure :tight @14:50:06.34 Elbow.seek CC @14:50:06.56 Elbow.reconfigure :loose @14:50:06.66 Forearm.seek CC @14:50:07.64 Hand.close @14:50:08.44 CC.seek :open @14:50:12.54 Forearm.seek :retract @14:50:13.57 Elbow.reconfigure :tight @14:50:13.62 Elbow.seek :garage @14:50:16.45 Elbow.reconfigure :loose @14:50:16.55 Forearm.reconfigure :tight @14:50:16.60 Forearm.seek :garage @14:50:17.76 Hand.open @14:50:18.50 Forearm.reconfigure :loose @14:50:18.55 Forearm.seek :retract @14:50:20.07 delayUntil "14:54:27.35" @14:50:20.09 Whole Cell Archive sampling 50ml @14:50:20.12 Loading wcr puck from tube 2 into Collection Clamp @14:50:20.37 SC.reconfigure :tight @14:50:20.42 SC.seek 2 @14:50:20.53 SC.configure :limp @14:50:20.75 Elbow.reconfigure :tight @14:50:20.80 Elbow.seek 2 @14:50:22.79 Forearm.reconfigure :tight @14:50:22.84 Forearm.seek 2 + 800 counts @14:50:23.92 SE.reconfigure :pick @14:50:23.98 SE.seek :beamBreak @14:50:26.83 SE.configure :default @14:50:27.00 Elbow.seek 2 @14:50:27.26 Forearm.seek 2 + 300 counts @14:50:27.56 Forearm.reconfigure :loose @14:50:27.61 Hand.close @14:50:28.33 SE.seek :down @14:50:28.54 Forearm.seek :retract @14:50:29.51 Elbow.seek CC @14:50:31.12 Elbow.reconfigure :loose @14:50:31.19 SE.stop @14:50:31.32 Forearm.reconfigure :tight @14:50:31.37 Forearm.seek CC @14:50:33.18 CC.seek :closed @14:50:37.20 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.02 @14:50:37.22 Hand.open @14:50:38.00 Forearm.reconfigure :loose @14:50:38.05 Forearm.seek :retract @14:50:39.02 collectSample 50ml w/bubble point of 35psi @14:50:39.04 Priming 7ml @14:50:39.05 Intake.select :close @14:50:39.06 Exhaust.select :close @14:50:39.07 SS.hold @14:50:39.29 Calibrating pressure sensors @14:50:39.30 CTV.dial :air @14:50:39.32 SSV.dial :spit,avoiding: :exhaust @14:50:39.40 SS.reconfigure :fast @14:50:39.45 SS.seek 5ml @14:50:42.26 SSV.dial CBV @14:50:42.28 CBV.dial SSV @14:50:43.66 delay 3.8 seconds @14:50:47.59 Sensor limits: Intake max 86.3psia; Outlet max 104.7psia @14:50:47.61 SSV.dial :spit @14:50:48.96 SS.seek 0ml @14:50:51.78 SSV.dial CBV @14:50:53.11 CTV.dial :intake @14:50:53.89 SSV.dial CBV @14:50:53.91 CBV.dial SSV @14:50:54.07 Allowing 40s for 7.0ml of remaining 7.0ml @14:50:54.09 Intake.select :open @14:50:56.26 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:50:56.28 SS.reconfigure :inhaling 2.17ml/s @14:50:56.33 SS.seek 7ml @14:51:02.49 Stroke completed -- waiting for pressure to equalize ... @14:51:02.50 SS.reconfigure :exhale @14:51:02.55 SS.hold @14:51:06.34 Intake.select :close @14:51:06.50 SSV.dial :exhaust @14:51:07.27 Exhaust.select :open @14:51:07.39 SS.seek 0ml @14:51:11.36 Exhaust.select :close @14:51:11.47 Sampling 50.0ml @14:51:11.48 Intake.select :close @14:51:11.49 Exhaust.select :close @14:51:11.51 SS.reconfigure :default @14:51:11.55 SS.hold @14:51:11.76 CTV.dial :intake @14:51:11.95 SSV.dial CBV @14:51:11.98 CBV.dial SSV @14:51:12.77 Allowing 130s for 25.0ml of remaining 50.0ml @14:51:12.78 Intake.select :open @14:51:14.95 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:51:14.96 SS.reconfigure :inhaling 2.17ml/s @14:51:15.02 SS.seek 25ml @14:51:30.98 Stroke completed -- waiting for pressure to equalize ... @14:51:31.00 SS.reconfigure :exhale @14:51:31.05 SS.hold @14:51:42.19 Intake.select :close @14:51:42.36 SSV.dial :exhaust @14:51:43.13 Exhaust.select :open @14:51:43.25 SS.seek 0ml @14:51:52.11 Exhaust.select :close @14:51:52.22 SSV.dial CBV @14:51:52.26 CBV.dial SSV @14:51:53.02 Allowing 130s for 25.0ml of remaining 25.0ml @14:51:53.03 Intake.select :open @14:51:55.20 Intake @13.8psia, vacuum from 8.3 to 11.3psi @14:51:55.22 SS.reconfigure :inhaling 2.17ml/s @14:51:55.27 SS.seek 25ml @14:52:10.87 Stroke completed -- waiting for pressure to equalize ... @14:52:10.89 SS.reconfigure :exhale @14:52:10.94 SS.hold @14:52:23.72 Intake.select :close @14:52:23.88 SSV.dial :exhaust @14:52:24.64 Exhaust.select :open @14:52:24.76 SS.seek 0ml @14:52:33.97 Exhaust.select :close @14:52:34.08 Sampled 50.0ml @14:52:34.10 Drying puck before fixing for 4:00 @14:52:34.11 CBV.dial :waste2b @14:52:34.84 delay 1:00 @14:53:34.87 CSV.dial :air @14:53:35.33 CS.reconfigure :fast @14:53:35.38 CS.seek 10ml @14:53:59.26 Pumping wcr puck from tube 2 up to 35psi... @14:53:59.28 CBV.dial SSV @14:54:00.53 CBV.dial between CSV and waste2b @14:54:01.51 CSV.dial between air and CTV @14:54:01.83 CS.seek 7.183ml @14:54:09.17 CTV.dial CSV,avoiding: :air @14:54:09.20 CSV.dial CTV @14:54:10.03 CS.reconfigure :pressurize @14:54:10.08 CS.seek 0ml @14:54:24.93 CBV.dial :waste2b @14:54:25.28 CS.reconfigure :default @14:54:25.33 CS.seek 0ml @14:54:27.36 recoverPRVreagent08 @14:54:27.37 PTV.dial :mixing @14:54:28.12 PBV.dial :waste1 @14:54:28.51 delay 30 seconds @14:54:30.07 delay 1:00 @14:54:58.53 PBV.dial between PSV and waste1 @14:54:59.37 PSV.dial :air @14:54:59.84 PS.reconfigure :fast @14:54:59.89 PS.seek 2.5ml @14:55:23.71 PSV.dial :mixloop @14:55:24.18 delay 2 seconds @14:55:26.20 PSV.dial :air @14:55:26.66 delay 2 seconds @14:55:28.68 PSV.dial :mixloop @14:55:29.26 delay 2 seconds @14:55:30.09 CTV.dial :air,avoiding: :intake @14:55:31.28 PSV.dial :air @14:55:31.45 Fix #1 of 1 (with RNAlater) for 4:00 @14:55:31.47 CSV.dial CSR @14:55:31.73 PS.seek 0ml @14:55:31.90 Pulling 2ml of CSR.RNAlater @14:55:31.91 CSR.select :RNAlater @14:55:32.02 CS.seek 2ml @14:55:37.97 delay 5 seconds @14:55:43.00 CSR.select :air @14:55:43.12 CS.reconfigure :fast @14:55:43.17 CS.seek 10ml @14:55:55.15 washCycleDA #1 of 2 @14:55:55.16 PSR.select :air @14:55:55.18 PSV.dial PSR @14:55:56.49 Pulling 0.5ml of PSR.wash @14:55:56.50 PSR.select :wash @14:55:56.62 PS.reconfigure :default @14:55:56.67 PS.seek 0.5ml @14:56:02.38 Pumping wcr puck from tube 2 up to 35psi... @14:56:02.40 CBV.dial SSV @14:56:02.96 delay 5 seconds @14:56:03.64 CBV.dial between CSV and waste2b @14:56:04.59 CSV.dial between air and CTV @14:56:05.20 CS.seek 7.069ml @14:56:07.98 PSR.select :air @14:56:08.10 PS.seek 2ml @14:56:12.73 CTV.dial CSV,avoiding: :air @14:56:12.76 CSV.dial CTV @14:56:14.17 CS.reconfigure :pressurize @14:56:14.22 CS.seek 0ml @14:56:24.21 PTV.dial :puck @14:56:24.96 PSV.dial PTV @14:56:25.69 PBV.dial :waste1 @14:56:26.15 PS.seek 1.5ml @14:56:29.44 delay 4:00 @14:56:32.12 delay 3 seconds @14:56:35.14 PBV.dial between PSV and waste1 @14:56:35.53 PS.seek 0ml @14:56:51.72 delay 2:00 @14:58:51.75 recoverPSRreagent08 PTV.dial :puck @14:58:51.83 PBV.dial :waste1 @14:58:52.22 delay 30 seconds @14:59:22.25 PBV.dial between PSV and waste1 @14:59:22.65 PSR.select :air @14:59:22.66 PSV.dial PSR @14:59:23.42 PS.reconfigure :fast @14:59:23.47 PS.seek 2.5ml @14:59:46.99 PSV.dial PTV @14:59:47.74 delay 5 seconds @14:59:52.76 PSV.dial PSR @14:59:53.50 delay 5 seconds @14:59:58.53 PSV.dial PTV @14:59:59.27 delay 5 seconds @15:00:04.29 PSV.dial :air @15:00:05.06 PS.seek 0ml @15:00:28.49 washCycleDA #2 of 2 @15:00:28.50 PSR.select :air @15:00:28.52 PSV.dial PSR @15:00:29.46 CBV.dial :waste2b @15:00:29.79 CS.reconfigure :default @15:00:29.88 CS.seek 0ml @15:00:29.89 Pulling 0.5ml of PSR.wash @15:00:29.91 PSR.select :wash @15:00:30.07 PS.reconfigure :default @15:00:30.12 PS.seek 0.5ml @15:00:33.98 delay 1:00 @15:00:36.11 delay 5 seconds @15:00:41.13 PSR.select :air @15:00:41.25 PS.seek 2ml @15:00:57.36 PTV.dial :puck @15:00:57.44 PSV.dial PTV @15:00:58.18 PBV.dial :waste1 @15:00:58.63 PS.seek 1.5ml @15:01:04.55 delay 3 seconds @15:01:07.57 PBV.dial between PSV and waste1 @15:01:07.96 PS.seek 0ml @15:01:24.27 delay 2:00 @15:01:34.01 CTV.dial :air,avoiding: :intake @15:03:24.30 recoverPSRreagent08 @15:03:24.31 PTV.dial :puck @15:03:24.39 PBV.dial :waste1 @15:03:24.78 delay 30 seconds @15:03:54.81 PBV.dial between PSV and waste1 @15:03:55.20 PSR.select :air @15:03:55.22 PSV.dial PSR @15:03:55.98 PS.reconfigure :fast @15:03:56.03 PS.seek 2.5ml @15:04:19.45 PSV.dial PTV @15:04:20.20 delay 5 seconds @15:04:25.23 PSV.dial PSR @15:04:25.97 delay 5 seconds @15:04:30.99 PSV.dial PTV @15:04:31.75 delay 5 seconds @15:04:36.77 PSV.dial :air @15:04:37.52 PS.seek 0ml @15:05:00.92 getPMLReagent08 @15:05:00.93 PRV.dial :air @15:05:01.39 delay 5 seconds @15:05:06.41 PTV.dial :PRVmixing @15:05:08.17 delay 5 seconds @15:05:13.19 PRV.dial :ab2 @15:05:13.64 PTV.dial :PRVmixing @15:05:13.83 PSV.dial :mixloop @15:05:14.29 PS.reconfigure :default @15:05:14.34 PS.seek 0.3ml @15:05:18.30 delay 2 seconds @15:05:20.32 PRV.dial :air @15:05:20.84 PS.seek 0.55ml @15:05:24.33 addPMLReagent08 @15:05:24.34 PSV.dial :air @15:05:24.82 PS.seek 2ml @15:05:40.42 PSV.dial :PML @15:05:40.89 PTV.dial :mixing @15:05:41.64 PBV.dial :waste1 @15:05:42.10 PS.seek 1.85ml @15:05:44.42 PBV.dial between PSV and waste1 @15:05:44.80 PS.seek 0ml @15:06:04.58 delay 2 seconds @15:06:06.60 delay 8:00 @15:14:06.62 recoverPRVreagent08 @15:14:06.63 PTV.dial :mixing @15:14:06.80 PBV.dial :waste1 @15:14:07.18 delay 30 seconds @15:14:37.21 PBV.dial between PSV and waste1 @15:14:37.59 PSV.dial :air @15:14:38.06 PS.reconfigure :fast @15:14:38.11 PS.seek 2.5ml @15:15:01.57 PSV.dial :mixloop @15:15:02.04 delay 2 seconds @15:15:04.06 PSV.dial :air @15:15:04.52 delay 2 seconds @15:15:06.54 PSV.dial :mixloop @15:15:07.01 delay 2 seconds @15:15:09.03 PSV.dial :air @15:15:09.50 PS.seek 0ml @15:15:33.10 washCycleDA #1 of 3 @15:15:33.11 PSR.select :air @15:15:33.13 PSV.dial PSR @15:15:34.44 Pulling 0.5ml of PSR.wash @15:15:34.46 PSR.select :wash @15:15:34.57 PS.reconfigure :default @15:15:34.62 PS.seek 0.5ml @15:15:40.54 delay 5 seconds @15:15:45.56 PSR.select :air @15:15:45.68 PS.seek 2ml @15:16:01.79 PTV.dial :puck @15:16:02.54 PSV.dial PTV @15:16:03.27 PBV.dial :waste1 @15:16:03.71 PS.seek 1.5ml @15:16:09.60 delay 3 seconds @15:16:12.62 PBV.dial between PSV and waste1 @15:16:13.00 PS.seek 0ml @15:16:29.16 delay 2:00 @15:18:29.18 recoverPSRreagent08 @15:18:29.19 PTV.dial :puck @15:18:29.27 PBV.dial :waste1 @15:18:29.65 delay 30 seconds @15:18:59.67 PBV.dial between PSV and waste1 @15:19:00.50 PSR.select :air @15:19:00.52 PSV.dial PSR @15:19:01.28 PS.reconfigure :fast @15:19:01.33 PS.seek 2.5ml @15:19:24.91 PSV.dial PTV @15:19:25.66 delay 5 seconds @15:19:30.68 PSV.dial PSR @15:19:31.41 delay 5 seconds @15:19:36.43 PSV.dial PTV @15:19:37.18 delay 5 seconds @15:19:42.20 PSV.dial :air @15:19:42.96 PS.seek 0ml @15:20:06.66 washCycleDA #2 of 3 @15:20:06.67 PSR.select :air @15:20:06.69 PSV.dial PSR @15:20:08.00 Pulling 0.5ml of PSR.wash @15:20:08.01 PSR.select :wash @15:20:08.13 PS.reconfigure :default @15:20:08.18 PS.seek 0.5ml @15:20:14.16 delay 5 seconds @15:20:19.18 PSR.select :air @15:20:19.30 PS.seek 2ml @15:20:35.44 PTV.dial :puck @15:20:35.45 PSV.dial PTV @15:20:36.19 PBV.dial :waste1 @15:20:36.63 PS.seek 1.5ml @15:20:42.60 delay 3 seconds @15:20:45.62 PBV.dial between PSV and waste1 @15:20:45.99 PS.seek 0ml @15:21:02.19 delay 2:00 @15:23:02.22 recoverPSRreagent08 @15:23:02.23 PTV.dial :puck @15:23:02.24 PBV.dial :waste1 @15:23:02.61 delay 30 seconds @15:23:32.64 PBV.dial between PSV and waste1 @15:23:33.02 PSR.select :air @15:23:33.04 PSV.dial PSR @15:23:33.79 PS.reconfigure :fast @15:23:33.84 PS.seek 2.5ml @15:23:57.41 PSV.dial PTV @15:23:58.16 delay 5 seconds @15:24:03.18 PSV.dial PSR @15:24:03.93 delay 5 seconds @15:24:08.95 PSV.dial PTV @15:24:09.70 delay 5 seconds @15:24:14.73 PSV.dial :air @15:24:15.49 PS.seek 0ml @15:24:38.91 washCycleDA #3 of 3 @15:24:38.92 PSR.select :air @15:24:38.94 PSV.dial PSR @15:24:40.25 Pulling 0.5ml of PSR.wash @15:24:40.26 PSR.select :wash @15:24:40.38 PS.reconfigure :default @15:24:40.43 PS.seek 0.5ml @15:24:46.44 delay 5 seconds @15:24:51.46 PSR.select :air @15:24:51.58 PS.seek 2ml @15:25:07.75 PTV.dial :puck @15:25:07.76 PSV.dial PTV @15:25:08.50 PBV.dial :waste1 @15:25:08.94 PS.seek 1.5ml @15:25:14.91 delay 3 seconds @15:25:17.93 PBV.dial between PSV and waste1 @15:25:18.30 PS.seek 0ml @15:25:34.91 delay 2:00 @15:27:34.94 recoverPSRreagent08 @15:27:34.95 PTV.dial :puck @15:27:34.96 PBV.dial :waste1 @15:27:35.33 delay 30 seconds @15:28:05.36 PBV.dial between PSV and waste1 @15:28:05.73 PSR.select :air @15:28:05.75 PSV.dial PSR @15:28:06.50 PS.reconfigure :fast @15:28:06.56 PS.seek 2.5ml @15:28:30.25 PSV.dial PTV @15:28:31.00 delay 5 seconds @15:28:36.02 PSV.dial PSR @15:28:36.89 delay 5 seconds @15:28:41.91 PSV.dial PTV @15:28:42.65 delay 5 seconds @15:28:47.68 PSV.dial :air @15:28:48.44 PS.seek 0ml @15:29:11.87 Gate.power :camera,:ON @15:29:11.89 Mixing DA Substrates PSR.select :air @15:29:11.91 PSV.dial PSR @15:29:13.28 Pulling 0.25ml of PSR.sub1 @15:29:13.30 PSR.select :sub1 @15:29:13.52 PS.reconfigure :default @15:29:13.64 PS.seek 0.25ml @15:29:17.07 delay 5 seconds @15:29:22.09 Pulling 0.25ml of PSR.sub2 @15:29:22.10 PSR.select :sub2 @15:29:22.22 PS.seek 0.5ml @15:29:25.62 delay 5 seconds @15:29:30.64 PSR.select :air @15:29:30.76 PS.seek 2ml @15:29:47.00 PTV.dial :puck @15:29:47.01 PSV.dial PTV @15:29:47.76 PBV.dial :waste1 @15:29:48.21 PS.seek 1.5ml @15:29:54.18 delay 3 seconds @15:29:57.20 PBV.dial between PSV and waste1 @15:29:57.58 PS.seek 0ml @15:30:13.68 delay 10 seconds @15:30:23.71 recoverPSRreagent08 @15:30:23.72 PTV.dial :puck @15:30:23.73 PBV.dial :waste1 @15:30:24.12 delay 30 seconds @15:30:54.15 PBV.dial between PSV and waste1 @15:30:54.53 PSR.select :air @15:30:54.55 PSV.dial PSR @15:30:55.30 PS.reconfigure :fast @15:30:55.35 PS.seek 2.5ml @15:31:19.12 PSV.dial PTV @15:31:19.89 delay 5 seconds @15:31:24.91 PSV.dial PSR @15:31:25.67 delay 5 seconds @15:31:30.69 PSV.dial PTV @15:31:31.43 delay 5 seconds @15:31:36.45 PSV.dial :air @15:31:37.24 PS.seek 0ml @15:32:00.90 Additional air clear @15:32:00.91 PSR.select :air @15:32:00.93 PSV.dial PSR @15:32:02.26 Pulling 2ml of PSR.air @15:32:02.27 PSR.select :air @15:32:02.29 PS.reconfigure :default @15:32:02.34 PS.seek 2ml @15:32:23.59 delay 5 seconds @15:32:28.61 PTV.dial :puck @15:32:28.63 PSV.dial PTV @15:32:29.37 PBV.dial :waste1 @15:32:29.81 PS.seek 1.5ml @15:32:36.24 delay 3 seconds @15:32:39.26 PBV.dial between PSV and waste1 @15:32:39.64 delay 1 second @15:32:40.65 PSV.dial :waste1 @15:32:41.82 PS.seek 0ml @15:32:58.08 Processing Puck Heater at 28.1C before imaging @15:32:58.09 PH.coast @15:32:58.21 Imaging da puck in Processing Clamp while storing it to tube 1 @15:32:58.39 SC.reconfigure :tight @15:32:58.44 SC.seek 1 @15:33:02.04 SC.configure :limp @15:33:02.26 Elbow.reconfigure :tight @15:33:02.32 Elbow.seek 1 @15:33:04.01 Forearm.reconfigure :tight @15:33:04.06 Forearm.seek 1 + 800 counts @15:33:05.16 SE.reconfigure :pick @15:33:05.22 SE.seek :beamBreak @15:33:08.07 SE.configure :default @15:33:08.23 Elbow.reconfigure :limp @15:33:08.28 Forearm.reconfigure :loose @15:33:08.33 Forearm.seek 1 @15:33:08.78 Elbow.hold @15:33:08.86 Elbow.reconfigure :tight @15:33:08.97 SE.seek 1.1 Pucks below Top Plate @15:33:09.41 Forearm.force -20 @15:33:09.45 Forearm.seek 1 + 286 counts @15:33:09.73 Forearm.reconfigure :tight @15:33:09.79 Forearm.seek 1 + 800 counts @15:33:10.10 SE.seek 2.1 Pucks below Top Plate @15:33:10.65 Forearm.reconfigure :loose @15:33:10.70 Forearm.seek :retract @15:33:11.60 Elbow.seek PC @15:33:14.13 Elbow.reconfigure :loose @15:33:14.23 Forearm.seek PC @15:33:15.83 Hand.close @15:33:16.52 PC.seek :open @15:33:21.08 Forearm.seek :retract @15:33:22.10 Elbow.reconfigure :tight @15:33:22.15 Elbow.seek 1 @15:33:24.76 Forearm.reconfigure :tight @15:33:24.81 Forearm.seek 1 + 800 counts @15:33:26.04 Hand.open @15:33:26.83 SE.seek 1.85 Pucks below Top Plate @15:33:27.13 SE.reconfigure :seal @15:33:27.18 SE.seek 1.2 Pucks below Top Plate @15:33:27.72 Lights, camera, ACTION! @15:33:27.76 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da25aug2915h50ml.tif @15:33:47.23 Waiting >20 seconds for da to relinquish FlushPuck @15:33:54.73 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 23.435 seconds /var/log/esp/da25aug2915h50ml.tif: TIFF Upload Complete @15:33:54.75 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da25aug2915h50ml15s.tif @15:34:10.41 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da25aug2915h50ml15s.tif: TIFF Upload Complete @15:34:10.43 PuckCamera: snap -bin=2,2 22.0 /var/log/esp/midres/da25aug2915h50ml22s.tif @15:34:33.05 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 22 seconds /var/log/esp/midres/da25aug2915h50ml22s.tif: TIFF Upload Complete @15:34:33.06 Gate.power :camera,:OFF @15:34:33.08 DAfans.email "23.435 sec auto-exposure",Subject: "da25aug2915h50ml images!" @15:34:33.51 Elbow.seek 1 @15:34:33.92 Forearm.seek 1 + 800 counts @15:34:34.01 SE.reconfigure :gently @15:34:34.08 SE.seek 1.91 Pucks below Top Plate @15:34:34.82 Elbow.reconfigure :limp @15:34:34.88 Forearm.reconfigure :loose @15:34:34.94 Forearm.seek 1 @15:34:35.44 Elbow.hold @15:34:35.51 Elbow.reconfigure :tight @15:34:35.57 SE.reconfigure :default @15:34:35.62 SE.seek 2.1 Pucks below Top Plate @15:34:36.00 Forearm.force -20 @15:34:36.05 Forearm.seek 1 + 294 counts @15:34:37.17 Forearm.reconfigure :tight @15:34:37.33 Forearm.seek 1 + 800 counts @15:34:37.79 SE.seek :down @15:34:39.06 Forearm.reconfigure :loose @15:34:39.16 Forearm.seek :retract @15:34:40.31 SE.stop @15:34:40.55 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:34:41.07 Elbow.seek FlushPuck::Garage @15:34:42.96 Elbow.reconfigure :loose @15:34:43.20 Forearm.seek :garage @15:34:44.34 Hand.close @15:34:45.41 Forearm.seek :retract @15:34:46.60 Elbow.reconfigure :tight @15:34:46.70 Elbow.seek PC @15:34:48.13 Elbow.reconfigure :loose @15:34:48.34 Forearm.reconfigure :tight @15:34:48.44 Forearm.seek PC @15:34:50.18 PC.seek :closed @15:34:54.80 Processing Clamp onSpring/offSpring for 12/27 samples, on/off torque 1.59 @15:34:54.95 Hand.open @15:34:55.94 Forearm.reconfigure :loose @15:34:56.04 Forearm.seek :retract @15:34:57.10 PSR.select :air @15:34:57.16 Storing wcr puck in Collection Clamp to tube 1 @15:34:57.20 PSV.dial PSR @15:34:58.66 Pulling 0.5ml of PSR.flush @15:34:58.73 PSR.select :flush @15:34:59.16 PS.seek 0.5ml @15:34:59.59 Elbow.reconfigure :tight @15:34:59.72 Elbow.seek 1 @15:35:02.54 Forearm.reconfigure :tight @15:35:02.64 Forearm.seek 1 + 800 counts @15:35:03.80 SE.reconfigure :pick @15:35:03.92 SE.seek :beamBreak @15:35:05.15 delay 5 seconds @15:35:06.95 SE.configure :default @15:35:07.31 Elbow.reconfigure :limp @15:35:07.40 Forearm.reconfigure :loose @15:35:07.50 Forearm.seek 1 @15:35:07.91 Elbow.hold @15:35:08.07 Elbow.reconfigure :tight @15:35:08.30 SE.seek 2.1 Pucks below Top Plate @15:35:09.01 Forearm.force -20 @15:35:09.11 Forearm.seek 1 + 320 counts @15:35:09.57 Forearm.reconfigure :tight @15:35:09.68 Forearm.seek 1 + 800 counts @15:35:10.05 SE.seek 3.1 Pucks below Top Plate @15:35:10.18 PSR.select :air @15:35:10.34 PS.seek 2.5ml @15:35:10.85 Forearm.reconfigure :loose @15:35:10.94 Forearm.seek :retract @15:35:11.98 Elbow.seek CC @15:35:12.18 upsync @15:35:14.01 Elbow.reconfigure :loose @15:35:14.12 Forearm.seek CC @15:35:15.11 Hand.close @15:35:15.90 CC.seek :open @15:35:20.12 Forearm.seek :retract @15:35:21.12 Elbow.reconfigure :tight @15:35:21.17 Elbow.seek 1 @15:35:22.90 Elbow.reconfigure :limp @15:35:22.96 Forearm.seek 1 @15:35:24.14 Elbow.hold @15:35:24.21 Elbow.reconfigure :tight @15:35:24.26 Hand.open @15:35:25.14 Forearm.force -20 @15:35:25.19 Forearm.seek 1 + 298 counts @15:35:25.67 Forearm.reconfigure :tight @15:35:25.72 Forearm.seek 1 + 800 counts @15:35:26.07 SE.seek :down @15:35:27.21 Forearm.reconfigure :loose @15:35:27.26 Forearm.seek :retract @15:35:28.56 SE.stop @15:35:28.60
Starting finalPurge @15:35:31.59 delay 1 second @15:35:32.61 PRV.dial :waste2,avoiding: :ab2 @15:35:34.48 PTV.dial :PRVmixing @15:35:35.76 PSV.dial :mixloop @15:35:36.80 PS.seek :empty @15:35:48.56
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:36:03.15 PSR.select :air @15:36:03.17 PSV.dial PSR @15:36:04.63 Pulling 0.5ml of PSR.flush @15:36:04.64 PSR.select :flush @15:36:04.77 PS.seek 0.5ml @15:36:10.74 delay 5 seconds @15:36:15.76 PSR.select :air @15:36:15.88 PS.seek 2.5ml @15:36:37.49 delay 1 second @15:36:38.51 PBV.dial :waste1 @15:36:38.88 PTV.dial :mixing @15:36:39.62 PSV.dial :mixloop @15:36:40.67 PS.seek :empty @15:37:06.99 PSV.dial PSR @15:37:08.02 PSR.select :flush @15:37:08.14 PS.seek 1ml @15:37:19.21 PSR.select :air @15:37:19.32 PS.seek 2.5ml @15:37:35.55 PSV.dial PTV @15:37:36.30 PTV.dial :puck @15:37:37.04 PBV.dial :waste1 @15:37:37.12 PS.seek 0ml @15:38:03.43 PSV.dial PSR @15:38:04.16 PSR.select :flush @15:38:04.28 PS.seek 0.5ml @15:38:10.21 PSR.select :air @15:38:10.32 PS.seek 2.5ml @15:38:31.55 PSV.dial PTV @15:38:32.30 PBV.dial PSV @15:38:32.87 PS.seek 0ml @15:38:59.30 PSV.dial PBV @15:38:59.76 PS.seek 2.5ml @15:39:26.18 PSV.dial :waste1 @15:39:26.92 PS.seek 0ml @15:39:53.24 PSV.dial PSR @15:39:53.69 PSR.select :air @15:39:53.72 PS.seek 2.5ml @15:40:20.02 PSV.dial PTV @15:40:20.75 PBV.dial PSV @15:40:20.84 PS.seek 0ml @15:40:47.14 PSV.dial PBV @15:40:47.60 PS.seek 2.5ml @15:41:14.05 PSV.dial :waste1 @15:41:14.79 PS.seek 0ml @15:41:41.11 PSV.dial PSR @15:41:41.58 PSR.select :flush @15:41:41.70 PS.seek 1ml @15:41:52.77 PSR.select :air @15:41:53.32 PS.seek 2.5ml @15:42:09.42 PSV.dial PTV @15:42:10.16 PTV.dial :puck @15:42:10.24 PBV.dial :waste1 @15:42:10.81 PS.seek 0ml @15:42:37.11 PSV.dial PSR @15:42:37.97 PSR.select :flush @15:42:38.10 PS.seek 0.5ml @15:42:44.05 PSR.select :air @15:42:44.16 PS.seek 2.5ml @15:43:05.39 PSV.dial PTV @15:43:06.13 PBV.dial PSV @15:43:06.82 PS.seek 0ml @15:43:33.14 PSV.dial PBV @15:43:33.60 PS.seek 2.5ml @15:44:00.27 PSV.dial :waste1 @15:44:01.01 PS.seek 0ml @15:44:27.39 PSV.dial PSR @15:44:27.86 PSR.select :air @15:44:27.89 PS.seek 2.5ml @15:44:54.20 PSV.dial PTV @15:44:54.94 PBV.dial PSV @15:44:55.02 PS.seek 0ml @15:45:21.36 PSV.dial PBV @15:45:21.82 PS.seek 2.5ml @15:45:48.23 PSV.dial :waste1 @15:45:48.97 PS.seek 0ml @15:46:15.33
Loading FlushPuck into Collection Clamp from Processing Clamp @15:46:15.52 Elbow.seek PC @15:46:18.08 Elbow.reconfigure :loose @15:46:18.18 Forearm.seek PC @15:46:19.24 Hand.close @15:46:20.01 PC.seek :open @15:46:24.51 Forearm.seek :retract @15:46:25.54 Elbow.reconfigure :tight @15:46:25.59 Elbow.seek CC @15:46:29.20 Elbow.reconfigure :loose @15:46:29.69 Forearm.reconfigure :tight @15:46:29.80 Forearm.seek CC @15:46:31.14 CC.seek :closed @15:46:35.14 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.11 @15:46:35.21 Hand.open @15:46:36.07 Forearm.reconfigure :loose @15:46:36.11 Forearm.seek :retract @15:46:37.12 Flushing the Collection Valve::Manifold @15:46:37.25 CSV.dial CSR @15:46:37.71 Pulling 5ml of CSR.flush @15:46:37.72 CSR.select :flush @15:46:37.84 CS.seek 5ml @15:46:51.49 delay 5 seconds @15:46:56.51 CSR.select :air @15:46:56.68 CS.reconfigure :fast @15:46:56.73 CS.seek 7.5ml @15:47:03.49 CSV.dial :waste @15:47:04.76 CS.reconfigure :default @15:47:04.81 CS.seek 0ml @15:47:24.87 Flushing lower manifold's waste1 port @15:47:24.99 CSV.dial :flush @15:47:25.44 CS.reconfigure :fast @15:47:25.49 CS.seek 5ml @15:47:37.87 delay 5 seconds @15:47:42.89 CSV.dial :air @15:47:43.40 CS.seek 7ml @15:47:48.81 CSV.dial CTV @15:47:48.83
CTV.dial CSV @15:47:50.21 CBV.dial :waste1 @15:47:50.98 CS.seek 0ml @15:48:07.74 Flushing lower manifold's waste2 port @15:48:07.87 CSV.dial :flush @15:48:08.61 CS.seek 5ml @15:48:21.12 delay 5 seconds @15:48:26.14 CSV.dial :air @15:48:27.09 CS.seek 7ml @15:48:32.49 CSV.dial CTV @15:48:32.52
CTV.dial CSV @15:48:32.96 CBV.dial :waste2 @15:48:34.01 CS.seek 0ml @15:48:50.71 Flushing lower manifold's waste2b port @15:48:50.84 CSV.dial :flush @15:48:51.58 CS.seek 5ml @15:49:03.84 delay 5 seconds @15:49:08.85 CSV.dial :air @15:49:09.37 CS.seek 7ml @15:49:14.77 CSV.dial CTV @15:49:14.80
CTV.dial CSV @15:49:15.24 CBV.dial :waste2b @15:49:16.28 CS.seek 0ml @15:49:32.87 Disinfecting external Intake with kill @15:49:32.99 CSV.dial CSR @15:49:33.43 Pulling 8ml of CSR.kill @15:49:33.44 CSR.select :kill @15:49:33.55 CS.seek 8ml @15:49:52.65 delay 5 seconds @15:49:57.67 CSR.select :air @15:49:57.79 CS.seek 10ml @15:50:03.21 CSV.dial CBV @15:50:03.26
CBV.dial CSV @15:50:04.27 CTV.dial :intake @15:50:05.04 Intake.select :open @15:50:05.15 CS.seek 2ml @15:50:24.18 Intake.select :close @15:50:24.28 CTV.dial :air @15:50:25.06 CS.reconfigure :default @15:50:25.10 CS.seek 0ml @15:50:31.11 CSV.dial CSR @15:50:32.14 Pulling 5ml of CSR.flush @15:50:32.15 CSR.select :flush @15:50:32.27 CS.seek 5ml @15:50:45.80 delay 5 seconds @15:50:50.82 CSR.select :air @15:50:50.94 CS.seek 10ml @15:51:04.52 CSV.dial CBV @15:51:05.54 CS.seek 0ml @15:51:32.02 Garaging FlushPuck in Collection Clamp @15:51:32.09 Elbow.reconfigure :tight @15:51:32.14 Elbow.seek CC @15:51:32.34 Elbow.reconfigure :loose @15:51:32.44 Forearm.seek CC @15:51:33.47 Hand.close @15:51:34.25 CC.seek :open @15:51:38.42 Forearm.seek :retract @15:51:39.50 Elbow.reconfigure :tight @15:51:39.55 Elbow.seek :garage @15:51:42.37 Elbow.reconfigure :loose @15:51:42.47 Forearm.reconfigure :tight @15:51:42.52 Forearm.seek :garage @15:51:43.68 Hand.open @15:51:44.44 Forearm.reconfigure :loose @15:51:44.48 Forearm.seek :retract @15:51:45.50 Completed [initialPurge; da [50, 50] #da and wsr are run simultanelously] begun 14:00:46 @15:51:45.55 Asleep after completing DA_WCR_110124 assays @15:51:45.57 GoodNews.email "{1 => 4,\n 2 => 0}\nCTD data unavailable\nCan@15:49:59, 40% flow, 26.1C, 39% humidity, 14.8psia, 13.64V, 0.403A avg, 0.935Ah, 5.50W\nSampled 50.0ml",Subject: "Asleep after completing DA_WCR_110124 assays" @15:51:50.60 upsync + ESPwhich DA_WCR_110124.rb + fn=DA_WCR_110124.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_110124.rb + [ -f /home/esp/esp2/mission/DA_WCR_110124.rb ] + realpath /home/esp/esp2/mission/DA_WCR_110124.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_110124.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_110124.rb @14:38:26.94UTC02-Sep-25
>RESET< ESP2 v3.42 I2C Gateway v3.20 for core bus @14:38:29.58 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @14:38:33.21 Polling Can every 10:00 @14:38:33.27 Loading /home/esp/esp2local/mission/DA_WCR_110124.rb mission @14:38:33.33 Preparing to sleep until + 00:00:05 @14:38:33.36 GoodNews.email "Starting DA_WCR_110124 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@14:38:33, 40% flow, 25.2C, 41% humidity, 14.9psia, 13.81V, 0.089A avg, 0.142Ah, 1.23W",Subject: "DA_WCR_110124 begun" @14:38:33.38 Shutdown @14:38:33.41
Asleep til + 00:00:05 @14:38:33.43 Continuing at 14:38 @14:38:36.14 GoodNews.email "Then, in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_110124.rb:14\n{2 => 4}\nCTD data unavailable\nCan@14:38:33, 40% flow, 25.2C, 41% humidity, 14.9psia, 13.81V, 0.089A avg, 0.142Ah, 1.23W",Subject: "Asleep until 14:38" @14:38:37.86 upsync @14:38:38.29
Powering core bus @14:38:40.22 upsync @14:38:43.93
Gate.power :raw,:ON @14:38:44.78 CS.configure :default @14:38:44.83 PS.configure :default @14:38:44.88 SS.configure :default @14:38:44.93 CC.configure :default @14:38:44.97 PC.configure :default @14:38:45.04 Forearm.configure :loose @14:38:45.09 Elbow.configure :loose @14:38:45.16 SC.configure :loose @14:38:45.20 SE.configure :default @14:38:45.25 CH.configure :default @14:38:45.29 PH.configure :default @14:38:45.32 All dwarf controllers running firmware version 3.85 @14:38:45.43 Valve.reset @14:38:45.69 Intake.select :close @14:38:45.76 Exhaust.select :close @14:38:45.77 CTV.dial :air @14:38:45.88 PSV.dial :air @14:38:46.00
Forearm.home.seek :retract @14:38:46.01 CSV.dial :waste1 @14:38:46.49 SSV.dial :spit,avoiding: :exhaust @14:38:46.80 Hand.close @14:38:46.83
Elbow.reconfigure :loose @14:38:46.94 Elbow.jog 1500 @14:38:47.01 PS.home.seek 0ml @14:38:47.33 CS.reconfigure :fast @14:38:47.40 SS.home.seek 0ml @14:38:47.44 CS.home.seek 0ml @14:38:47.52
Elbow.reconfigure :loose @14:38:47.57 Elbow.home.seek :clear @14:38:50.75 SE.home.seek :down @14:38:51.40 SC.reconfigure :tight @14:38:51.45 SC.home.seek :initial @14:38:56.27 PS.stop @14:39:17.02
SC.configure :limp @14:39:17.07 Forearm.seek :retract @14:39:17.13 Elbow.seek :hammer @14:39:18.22 Forearm.reconfigure :tight @14:39:18.27 Forearm.seek retracted @14:39:18.77 CC.home.seek :open @14:39:18.78 PC.home.seek :open @14:39:22.28
Phase [initialPurge; da [50, 50] #da and wsr are run simultanelously] @14:39:22.29 Notice.email "At 14:39:22 began in tube 2: initialPurge; da [50, 50] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_110124.rb:14\n{2 => 4}\nCTD data unavailable\nCan@14:39:22, 40% flow, 25.2C, 41% humidity, 14.9psia, 13.72V, 0.259A avg, 0.148Ah, 3.55W",Subject: "Started DA_WCR_110124 phase initialPurge; da [50, 50] #da and wsr are run simultanelously" @14:39:22.32
Starting initialPurge @14:39:22.34 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:39:22.42 Hand.open @14:39:23.25 Elbow.reconfigure :tight @14:39:23.29 Elbow.seek FlushPuck::Garage @14:39:25.21 Elbow.reconfigure :loose @14:39:25.34 Forearm.reconfigure :loose @14:39:25.39 Forearm.seek :garage @14:39:26.41 Hand.close @14:39:26.49 upsync @14:39:27.23
Forearm.seek :retract @14:39:28.32 Elbow.reconfigure :tight @14:39:28.36 Elbow.seek CC @14:39:31.25 Elbow.reconfigure :loose @14:39:31.35 Forearm.reconfigure :tight @14:39:31.40 Forearm.seek CC @14:39:32.76 CC.seek :closed @14:39:36.75 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.09 @14:39:36.84 Hand.open @14:39:37.68 Forearm.reconfigure :loose @14:39:37.73 Forearm.seek :retract @14:39:38.67 Flushing the Collection Valve::Manifold @14:39:38.80 CSV.dial CSR @14:39:40.06 Pulling 5ml of CSR.flush @14:39:40.07 CSR.select :flush @14:39:40.18 CS.reconfigure :default @14:39:40.23 CS.seek 5ml @14:39:54.48 delay 5 seconds @14:39:59.50 CSR.select :air @14:39:59.66 CS.reconfigure :fast @14:39:59.71 CS.seek 7.5ml @14:40:06.32 CSV.dial :waste @14:40:07.60 CS.reconfigure :default @14:40:07.64 CS.seek 0ml @14:40:27.64 Purging external Intake @14:40:27.76 CSV.dial :flush @14:40:28.21 CS.reconfigure :fast @14:40:28.25 CS.seek 10ml @14:40:52.14 delay 5 seconds @14:40:57.16 CSV.dial CBV @14:40:57.19
CBV.dial CSV @14:40:57.93 CTV.dial :intake @14:40:58.70 Intake.select :open @14:40:58.81 CS.seek 0ml @14:41:22.54 Intake.select :close @14:41:22.65 CTV.dial :air @14:41:23.41 Flushing upper manifold @14:41:23.52 CSV.dial :flush @14:41:24.24 CS.seek 5ml @14:41:36.48 delay 5 seconds @14:41:41.50 CSV.dial :air @14:41:42.00 CS.seek 7ml @14:41:47.51 CSV.dial CBV @14:41:47.54
CBV.dial CSV @14:41:48.54 CTV.dial :air @14:41:48.56 CS.seek 0ml @14:42:05.39 delay 7 seconds @14:42:12.42 CS.seek 3ml @14:42:20.17 CSV.dial :waste @14:42:20.63 CS.seek 0ml @14:42:28.44 CSV.dial :flush @14:42:28.88 CS.seek 3ml @14:42:36.67 delay 5 seconds @14:42:41.69 CSV.dial :air @14:42:42.18 CS.seek 5ml @14:42:47.63 CSV.dial :air @14:42:47.83 CS.seek 0ml @14:43:00.04 delay 7 seconds @14:43:07.07 CS.seek 3ml @14:43:14.76 CSV.dial :waste @14:43:15.47 CS.seek 0ml @14:43:23.14 Garaging FlushPuck in Collection Clamp @14:43:23.21 Elbow.reconfigure :tight @14:43:23.25 Elbow.seek CC @14:43:23.43 Elbow.reconfigure :loose @14:43:23.52 Forearm.seek CC @14:43:24.54 Hand.close @14:43:25.23 CC.seek :open @14:43:29.65 Forearm.seek :retract @14:43:30.69 Elbow.reconfigure :tight @14:43:30.73 Elbow.seek :garage @14:43:33.58 Elbow.reconfigure :loose @14:43:33.68 Forearm.reconfigure :tight @14:43:33.73 Forearm.seek :garage @14:43:34.73 Hand.open @14:43:35.54 Forearm.reconfigure :loose @14:43:35.59 Forearm.seek :retract @14:43:36.66 da sampling at most 50ml, wcr at most 50ml @14:43:36.69 DAfans.email "Started da in tube 2 at 14:43:36UTC02-Sep-25\n{2 => 4}\nCTD data unavailable\nCan@14:39:22, 40% flow, 25.2C, 41% humidity, 14.9psia, 13.72V, 0.259A avg, 0.148Ah, 3.55W",Subject: "da sampling at most 50ml, wcr at most 50ml" @14:43:36.71
Loading sh1 puck from tube 2 into Collection Clamp @14:43:36.97 SC.reconfigure :tight @14:43:37.02 SC.seek 2 @14:43:40.97 upsync @14:43:43.82
SC.configure :limp @14:43:44.05 Elbow.reconfigure :tight @14:43:44.10 Elbow.seek 2 @14:43:46.05 Forearm.reconfigure :tight @14:43:46.10 Forearm.seek 2 + 800 counts @14:43:47.14 SE.reconfigure :pick @14:43:47.20 SE.seek :beamBreak @14:43:49.93 SE.configure :default @14:43:50.10 Elbow.seek 2 @14:43:50.28 Forearm.seek 2 + 300 counts @14:43:50.59 Forearm.reconfigure :loose @14:43:50.64 Hand.close @14:43:51.37 SE.seek :down @14:43:51.57
Forearm.seek :retract @14:43:52.54 Elbow.seek CC @14:43:54.01 SE.stop @14:43:54.13
Elbow.reconfigure :loose @14:43:54.23 Forearm.reconfigure :tight @14:43:54.28 Forearm.seek CC @14:43:55.72 CC.seek :closed @14:43:59.71 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 2.09 @14:43:59.72 Hand.open @14:44:00.54 Forearm.reconfigure :loose @14:44:00.59 Forearm.seek :retract @14:44:01.55 collectSample 50ml w/bubble point of 24psi @14:44:01.56 Priming 7ml @14:44:01.57 Intake.select :close @14:44:01.58 Exhaust.select :close @14:44:01.60 SS.hold @14:44:01.81 Calibrating pressure and temperature sensors @14:44:01.83 CTV.dial :air @14:44:01.85 SSV.dial :spit,avoiding: :exhaust @14:44:01.87 SS.reconfigure :fast @14:44:01.91 SS.seek 5ml @14:44:05.11 SSV.dial CBV @14:44:05.15
CBV.dial SSV @14:44:06.50 delay 3.8 seconds @14:44:10.43 Sensor limits: Intake max 75.9psia; Outlet max 104.7psia @14:44:10.44 SSV.dial :spit @14:44:11.79 SS.seek 0ml @14:44:14.53 SSV.dial CBV @14:44:15.87 CTV.dial :intake @14:44:16.64 SSV.dial CBV @14:44:16.66
CBV.dial SSV @14:44:16.85 Allowing 40s for 7.0ml of remaining 7.0ml @14:44:16.86 Intake.select :open @14:44:19.03 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:44:19.04 SS.reconfigure :inhaling 2.17ml/s @14:44:19.09 SS.seek 7ml @14:44:24.87 Stroke completed -- waiting for pressure to equalize ... @14:44:24.88 SS.reconfigure :exhale @14:44:24.93 SS.hold @14:44:25.27 Intake.select :close @14:44:25.43 SSV.dial :exhaust @14:44:26.19 Exhaust.select :open @14:44:26.30 SS.seek 0ml @14:44:30.27 Exhaust.select :close @14:44:30.38 Sampling 50.0ml @14:44:30.39 Intake.select :close @14:44:30.40 Exhaust.select :close @14:44:30.42 SS.reconfigure :default @14:44:30.46 SS.hold @14:44:31.10 CTV.dial :intake @14:44:31.26 SSV.dial CBV @14:44:31.28
CBV.dial SSV @14:44:32.08 Allowing 130s for 25.0ml of remaining 50.0ml @14:44:32.09 Intake.select :open @14:44:34.26 Intake @14.2psia, vacuum from 8.7 to 11.7psi @14:44:34.28 SS.reconfigure :inhaling 2.17ml/s @14:44:34.33 SS.seek 25ml @14:44:50.02 Stroke completed -- waiting for pressure to equalize ... @14:44:50.03 SS.reconfigure :exhale @14:44:50.08 SS.hold @14:44:54.83 Intake.select :close @14:44:54.98 SSV.dial :exhaust @14:44:55.74 Exhaust.select :open @14:44:55.84 SS.seek 0ml @14:45:05.13 Exhaust.select :close @14:45:05.23 SSV.dial CBV @14:45:05.25
CBV.dial SSV @14:45:06.03 Allowing 130s for 25.0ml of remaining 25.0ml @14:45:06.04 Intake.select :open @14:45:08.21 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:45:08.23 SS.reconfigure :inhaling 2.17ml/s @14:45:08.28 SS.seek 25ml @14:45:23.08 Stroke completed -- waiting for pressure to equalize ... @14:45:23.09 SS.reconfigure :exhale @14:45:23.13 SS.hold @14:45:29.19 Intake.select :close @14:45:29.35 SSV.dial :exhaust @14:45:30.11 Exhaust.select :open @14:45:30.23 SS.seek 0ml @14:45:39.56 Exhaust.select :close @14:45:39.67 Sampled 50.0ml @14:45:39.69 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:45:39.70 Intake.select :close @14:45:39.76 CSV.dial :waste @14:45:39.88 SSV.dial CBV,avoiding: :exhaust @14:45:39.91
CBV.dial SSV @14:45:40.04 CS.seek 0ml @14:45:40.11 CSV.dial :air @14:45:40.95 CS.seek 10ml @14:45:41.85
CTV.dial :air @14:45:42.60 delay 15 seconds @14:45:57.62 SSV.dial :spit,avoiding: :exhaust @14:45:58.98 CTV.dial CSV,avoiding: :intake @14:45:59.01
SS.seek 0ml @14:45:59.14 SSV.dial CBV,avoiding: :exhaust @14:46:05.01 SSV.dial between CBV and plug @14:46:05.50 CBV.dial between SSV and waste2 @14:46:05.92 CTV.dial CSV,avoiding: :air @14:46:05.94
CSV.dial CTV @14:46:06.43 CS.reconfigure :pressurize @14:46:06.48 CS.seek 0ml @14:46:27.29 BubblePoint: Intake @24.2psia, Outlet @0.9psia @14:46:27.30 CBV.dial SSV @14:46:27.65 SS.reconfigure :default @14:46:27.70 SS.seek 10ml @14:46:33.36 delay 15 seconds @14:46:48.49 Evacuated: Intake @23.7psia, Outlet @23.7psia @14:46:48.50 SSV.dial :spit,avoiding: :exhaust @14:46:49.55 SS.reconfigure :exhale @14:46:49.60 SS.seek 0ml @14:46:54.64 SS.reconfigure :fast @14:46:54.69 SS.seek 5ml @14:46:57.47 SSV.dial CBV,avoiding: :exhaust @14:46:57.49
CBV.dial SSV @14:46:58.88 CTV.dial :air,avoiding: :intake @14:47:00.21 CSV.dial CTV @14:47:00.25
SS.seek 10ml @14:47:01.37 CSV.dial :air @14:47:01.82 CS.reconfigure :fast @14:47:01.87 CS.seek 0ml @14:47:03.13
SSV.dial :spit,avoiding: :exhaust @14:47:04.46 SS.reconfigure :exhale @14:47:04.51 SS.seek 0ml @14:47:07.38 CS.stop @14:47:09.95
SS.reconfigure :fast @14:47:09.99 SS.seek 5ml @14:47:12.90 SSV.dial CBV,avoiding: :exhaust @14:47:12.92
CBV.dial SSV @14:47:15.32 SSV.dial :spit,avoiding: :exhaust @14:47:16.66 SS.reconfigure :default @14:47:16.71 SS.seek 0ml @14:47:19.93 SS.stop @14:47:19.98 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:47:19.99 Intake.select :close @14:47:20.05 CSV.dial :waste @14:47:20.19 SSV.dial CBV,avoiding: :exhaust @14:47:20.21
CBV.dial SSV @14:47:20.81 CS.seek 0ml @14:47:20.88 CSV.dial :air @14:47:21.59
CTV.dial :air @14:47:21.66 delay 15 seconds @14:47:21.73 CS.seek 10ml @14:47:36.68
SSV.dial :spit,avoiding: :exhaust @14:47:38.02 CTV.dial CSV,avoiding: :intake @14:47:38.05
SS.reconfigure :exhale @14:47:38.15 SS.seek 0ml @14:47:38.52 SSV.dial CBV,avoiding: :exhaust @14:47:45.57 SSV.dial between CBV and plug @14:47:46.07 CBV.dial between SSV and waste2 @14:47:46.46 CSV.dial between air and CTV @14:47:46.78 CS.seek 8.49ml @14:47:51.08 CTV.dial CSV,avoiding: :air @14:47:51.10
CSV.dial CTV @14:47:51.43 CS.reconfigure :pressurize @14:47:51.48 CS.seek 0ml @14:48:11.29 BubblePoint: Intake @24.2psia, Outlet @0.1psia @14:48:11.30 CBV.dial SSV @14:48:11.65 SS.reconfigure :default @14:48:11.70 SS.seek 10ml @14:48:17.47 delay 15 seconds @14:48:32.60 Evacuated: Intake @23.9psia, Outlet @10.9psia @14:48:32.61 SSV.dial :spit,avoiding: :exhaust @14:48:33.67 SS.reconfigure :exhale @14:48:33.72 SS.seek 0ml @14:48:38.86 SS.reconfigure :fast @14:48:38.90 SS.seek 5ml @14:48:41.80 SSV.dial CBV,avoiding: :exhaust @14:48:41.82
CBV.dial SSV @14:48:43.17 CTV.dial :air,avoiding: :intake @14:48:44.51 CSV.dial CTV @14:48:44.54
SS.seek 10ml @14:48:45.67 CSV.dial :air @14:48:46.49 CS.reconfigure :fast @14:48:46.61 CS.seek 0ml @14:48:47.01
SSV.dial :spit,avoiding: :exhaust @14:48:48.35 SS.reconfigure :exhale @14:48:48.40 SS.seek 0ml @14:48:49.63 CS.stop @14:48:53.52
SS.reconfigure :fast @14:48:53.57 SS.seek 5ml @14:48:56.69 SSV.dial CBV,avoiding: :exhaust @14:48:56.71
CBV.dial SSV @14:48:59.07 SSV.dial :spit,avoiding: :exhaust @14:49:00.39 SS.reconfigure :default @14:49:00.44 SS.seek 0ml @14:49:03.46 SS.stop @14:49:03.51 Lysing: Adding 2.0ml AqMeOH @14:49:03.52 CBV.dial between CSV and waste1,avoiding: :DBV @14:49:05.01 CTV.dial CSV @14:49:06.35 CSV.dial CSR @14:49:07.14 Pulling 1ml of CSR.AqMeOH @14:49:07.15 CSR.select :AqMeOH @14:49:07.26 CS.reconfigure :default @14:49:07.31 CS.seek 1ml @14:49:10.76 delay 5 seconds @14:49:15.77 CSR.select :air @14:49:15.94 CS.seek 6ml @14:49:29.50 delay 2 seconds @14:49:31.52 CSV.dial :waste2 @14:49:31.96 CS.reconfigure :fast @14:49:32.01 CS.seek 0ml @14:49:46.44 CSV.dial CSR @14:49:46.89 Pulling 2ml of CSR.AqMeOH @14:49:46.90 CSR.select :AqMeOH @14:49:47.02 CS.reconfigure :default @14:49:47.06 CS.seek 2ml @14:49:52.97 delay 5 seconds @14:49:57.99 CSR.select :air @14:49:58.16 CS.seek 5ml @14:50:06.85 delay 2 seconds @14:50:08.86 CSV.dial CTV @14:50:09.31 CS.seek 1ml @14:50:20.43 Before lysing Collection Puck Heater at 23.1C/15.1psia @14:50:20.45 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @14:50:20.47 CH.seek 70C,8:00 @14:52:26.75 CH.hold 10:00,70C @14:52:26.95 Begun lysing Collection Puck Heater at 68C/21.2psia @15:02:26.97 Ended lysing Collection Puck Heater at 70C/14.3psia @15:02:26.98 CS.seek 0ml @15:02:30.44 CTV.dial between spare and air,avoiding: :intake @15:02:31.48 CSV.dial :air @15:02:31.93 CS.seek 5ml @15:02:45.59 CSV.dial CTV @15:02:46.04 delay 2 seconds @15:02:48.06 CSV.dial :air @15:02:48.95 delay 2 seconds @15:02:50.97 CSV.dial CTV @15:02:51.43 delay 2 seconds @15:02:53.45 CSV.dial :air @15:02:53.90 delay 2 seconds @15:02:55.92 CS.seek 0ml @15:03:09.64 Puck @23.4psia after final lysis pressurization @15:03:09.65 Recovering Lysate into Collection syringe @15:03:09.66 CSV.dial CBV @15:03:10.66 CBV.dial CSV @15:03:11.04 CS.seek 5ml @15:03:24.71 delay 5 seconds @15:03:29.73 CTV.dial :air @15:03:30.32 CS.seek 7ml @15:03:36.34 delay 5 seconds @15:03:41.36 CSV.dial :air2 @15:03:41.80 CS.seek 10ml @15:03:50.37 CSV.dial between air and CTV,avoiding: CBV @15:03:51.51 Lysing Complete @15:03:51.58 Storing sh1 puck in Collection Clamp to tube 1 @15:03:51.75 SC.reconfigure :tight @15:03:51.80 SC.seek 1 @15:03:55.32 SC.configure :limp @15:03:55.55 Elbow.reconfigure :tight @15:03:55.59 Elbow.seek 1 @15:03:57.33 Forearm.reconfigure :tight @15:03:57.37 Forearm.seek 1 + 800 counts @15:03:58.61 SE.reconfigure :pick @15:03:58.67 SE.seek :beamBreak @15:04:01.47 SE.configure :default @15:04:01.52 SE.seek :place @15:04:01.99 Forearm.reconfigure :loose @15:04:02.04 Forearm.seek :retract @15:04:02.96 Elbow.seek CC @15:04:04.65 Elbow.reconfigure :loose @15:04:04.75 Forearm.seek CC @15:04:05.75 Hand.close @15:04:06.47 CC.seek :open @15:04:11.10 Forearm.seek :retract @15:04:12.15 Elbow.reconfigure :tight @15:04:12.20 Elbow.seek 1 @15:04:13.90 Elbow.reconfigure :limp @15:04:13.95 Forearm.seek 1 @15:04:15.10 Elbow.hold @15:04:15.17 Elbow.reconfigure :tight @15:04:15.22 Hand.open @15:04:16.08 Forearm.force -20 @15:04:16.12 Forearm.seek 1 + 319 counts @15:04:16.51 Forearm.reconfigure :tight @15:04:16.56 Forearm.seek 1 + 800 counts @15:04:16.84 SE.seek :down @15:04:17.97
Forearm.reconfigure :loose @15:04:18.02 Forearm.seek :retract @15:04:19.58 SE.stop @15:04:19.63
Tranferring DA @15:04:19.64 Beginning samplePassC @15:04:19.65 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:04:19.85 Elbow.seek FlushPuck::Garage @15:04:21.62 Elbow.reconfigure :loose @15:04:21.72 Forearm.seek :garage @15:04:22.69 Hand.close @15:04:23.58 Forearm.seek :retract @15:04:24.65 Elbow.reconfigure :tight @15:04:24.69 Elbow.seek CC @15:04:27.55 Elbow.reconfigure :loose @15:04:27.65 Forearm.reconfigure :tight @15:04:27.69 Forearm.seek CC @15:04:29.04 CC.seek :closed @15:04:33.04 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 2.01 @15:04:33.12 Hand.open @15:04:33.90 Forearm.reconfigure :loose @15:04:33.95 Forearm.seek :retract @15:04:34.96 PSV.dial PSR @15:04:36.45 Pulling 1.5ml of PSR.flush @15:04:36.46 PSR.select :flush @15:04:36.58 PS.seek 1.5ml @15:04:52.79 delay 5 seconds @15:04:57.81 PSV.dial :waste1 @15:04:58.28 PS.seek 0ml @15:05:14.41 PSV.dial PSR @15:05:14.88 PSR.select :air @15:05:15.00 PS.seek 2.5ml @15:05:41.42 PRV.dial CBV,avoiding: :ab2 @15:05:41.45
CBV.dial PRV @15:05:43.19 PSV.dial :mixloop @15:05:44.23 CTV.dial :air,avoiding: :intake @15:05:44.30 PTV.dial :PRVmixing @15:05:45.61 PS.seek 0ml @15:06:11.97 PSV.dial :air @15:06:12.43 PS.seek 2.5ml @15:06:38.78 PSV.dial :mixloop @15:06:39.24 PS.seek 0ml @15:07:05.63 PSV.dial :air @15:07:06.09 PS.seek 2.5ml @15:07:32.41 PSV.dial :mixloop @15:07:32.87 PS.seek 0ml @15:07:59.19 Garaging FlushPuck in Collection Clamp @15:07:59.26 Elbow.reconfigure :tight @15:07:59.31 Elbow.seek CC @15:07:59.42 Elbow.reconfigure :loose @15:07:59.52 Forearm.seek CC @15:08:00.51 Hand.close @15:08:01.21 CC.seek :open @15:08:05.71 Forearm.seek :retract @15:08:06.86 Elbow.reconfigure :tight @15:08:06.91 Elbow.seek :garage @15:08:09.75 Elbow.reconfigure :loose @15:08:09.85 Forearm.reconfigure :tight @15:08:09.90 Forearm.seek :garage @15:08:11.08 Hand.open @15:08:11.89 Forearm.reconfigure :loose @15:08:11.94 Forearm.seek :retract @15:08:13.05 PSV.dial :mixloop @15:08:13.13 PTV.dial :PRVmixing @15:08:13.21 PRV.dial CBV @15:08:13.23
CBV.dial PRV @15:08:13.37 CTV.dial :air,avoiding: :intake @15:08:13.45 Loading lyfil puck from tube 2 into Collection Clamp @15:08:13.68 SC.reconfigure :tight @15:08:13.72 SC.seek 2 @15:08:17.65 SC.configure :limp @15:08:17.86 Elbow.reconfigure :tight @15:08:17.91 Elbow.seek 2 @15:08:19.79 Forearm.reconfigure :tight @15:08:19.84 Forearm.seek 2 + 800 counts @15:08:20.81 SE.reconfigure :pick @15:08:20.87 SE.seek :beamBreak @15:08:23.67 SE.configure :default @15:08:23.82 Elbow.seek 2 @15:08:24.01 Forearm.seek 2 + 300 counts @15:08:24.47 Forearm.reconfigure :loose @15:08:24.51 Hand.close @15:08:25.22 SE.seek :down @15:08:25.85
Forearm.seek :retract @15:08:26.89 Elbow.seek CC @15:08:27.85 SE.stop @15:08:28.45
Elbow.reconfigure :loose @15:08:28.55 Forearm.reconfigure :tight @15:08:28.60 Forearm.seek CC @15:08:29.91 CC.seek :closed @15:08:33.87 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.06 @15:08:33.88 Hand.open @15:08:34.64 Forearm.reconfigure :loose @15:08:34.69 Forearm.seek :retract @15:08:35.77 CTV.dial CSV,avoiding: :intake @15:08:37.13 CSV.dial CTV @15:08:37.45 PS.seek 2.5ml @15:09:03.84 CS.seek 5ml @15:09:17.55 delay 8 seconds @15:09:25.57 PSV.dial :air @15:09:26.14 delay 2 seconds @15:09:28.16 PSV.dial :waste2 @15:09:28.91 PS.seek 0ml @15:09:55.25 CBV.dial between PRV and waste1 @15:09:55.58 CSV.dial :air @15:09:56.04 CS.seek 10ml @15:10:09.70 CSV.dial CTV @15:10:10.17 CS.seek 0ml @15:10:36.55 CBV.dial :waste1 @15:10:36.86 delay 5 seconds @15:10:41.89 CTV.dial :air,avoiding: :intake @15:10:43.30 Storing lyfil puck in Collection Clamp to tube 1 @15:10:43.92 SC.reconfigure :tight @15:10:43.96 SC.seek 1 @15:10:47.49 SC.configure :limp @15:10:47.71 Elbow.reconfigure :tight @15:10:47.76 Elbow.seek 1 @15:10:49.45 Forearm.reconfigure :tight @15:10:49.49 Forearm.seek 1 + 800 counts @15:10:50.56 SE.reconfigure :pick @15:10:50.62 SE.seek :beamBreak @15:10:53.49 SE.configure :default @15:10:53.65 Elbow.reconfigure :limp @15:10:53.70 Forearm.reconfigure :loose @15:10:53.75 Forearm.seek 1 @15:10:54.12 Elbow.hold @15:10:54.19 Elbow.reconfigure :tight @15:10:54.29 SE.seek 0.1 Pucks below Top Plate @15:10:54.74 Forearm.force -20 @15:10:54.79 Forearm.seek 1 + 316 counts @15:10:55.20 Forearm.reconfigure :tight @15:10:55.24 Forearm.seek 1 + 800 counts @15:10:55.55 SE.seek 1.1 Pucks below Top Plate @15:10:56.09 Forearm.reconfigure :loose @15:10:56.14 Forearm.seek :retract @15:10:57.05 Elbow.seek CC @15:10:58.66 Elbow.reconfigure :loose @15:10:58.76 Forearm.seek CC @15:10:59.78 Hand.close @15:11:00.45 CC.seek :open @15:11:05.10 Forearm.seek :retract @15:11:06.27 Elbow.reconfigure :tight @15:11:06.31 Elbow.seek 1 @15:11:08.04 Elbow.reconfigure :limp @15:11:08.09 Forearm.seek 1 @15:11:09.22 Elbow.hold @15:11:09.28 Elbow.reconfigure :tight @15:11:09.33 Hand.open @15:11:10.21 Forearm.force -20 @15:11:10.25 Forearm.seek 1 + 313 counts @15:11:10.62 Forearm.reconfigure :tight @15:11:10.67 Forearm.seek 1 + 800 counts @15:11:10.99 SE.seek :down @15:11:12.12
Forearm.reconfigure :loose @15:11:12.17 Forearm.seek :retract @15:11:13.58 SE.stop @15:11:13.62
PSV.dial :air @15:11:14.35 PS.seek 2.5ml @15:11:40.75 delay 1 second @15:11:41.77 PSV.dial :mixloop @15:11:42.22 delay 1 second @15:11:43.24 PSV.dial :air @15:11:43.70 delay 1 second @15:11:44.72 PSV.dial :mixloop @15:11:45.19 delay 1 second @15:11:46.21 PSV.dial :air @15:11:46.67 delay 1 second @15:11:47.69 PS.seek 0ml @15:12:14.03 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:12:14.22 Elbow.seek FlushPuck::Garage @15:12:16.01 Elbow.reconfigure :loose @15:12:16.55 Forearm.seek :garage @15:12:17.65 Hand.close @15:12:18.57 Forearm.seek :retract @15:12:19.58 Elbow.reconfigure :tight @15:12:19.63 Elbow.seek PC @15:12:20.90 Elbow.reconfigure :loose @15:12:21.00 Forearm.reconfigure :tight @15:12:21.05 Forearm.seek PC @15:12:22.43 PC.seek :closed @15:12:26.85 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.57 @15:12:26.92 Hand.open @15:12:27.76 Forearm.reconfigure :loose @15:12:27.81 Forearm.seek :retract @15:12:28.82 PRV.dial :air @15:12:29.27 PSV.dial :mixloop @15:12:29.80 PS.seek 0.3ml @15:12:33.75 PSV.dial PSR @15:12:34.79 Pulling 0.4ml of PSR.air @15:12:34.80 PSR.select :air @15:12:34.82 PS.seek 0.7ml @15:12:39.66 delay 3 seconds @15:12:42.67 PSR.select :air @15:12:42.70 PS.seek 2.5ml @15:13:01.97 delay 3 seconds @15:13:04.99 PSV.dial :mixloop @15:13:06.03 PTV.dial :mixing @15:13:06.76 PBV.dial :waste2 @15:13:07.76 PS.seek 0ml @15:13:34.06 Garaging FlushPuck in Processing Clamp @15:13:34.13 Elbow.reconfigure :tight @15:13:34.56 Elbow.seek PC @15:13:34.75 Elbow.reconfigure :loose @15:13:34.85 Forearm.seek PC @15:13:35.98 Hand.close @15:13:36.70 PC.seek :open @15:13:41.21 Forearm.seek :retract @15:13:42.28 Elbow.reconfigure :tight @15:13:42.33 Elbow.seek :garage @15:13:43.66 Elbow.reconfigure :loose @15:13:43.76 Forearm.reconfigure :tight @15:13:43.81 Forearm.seek :garage @15:13:44.99 Hand.open @15:13:45.76 Forearm.reconfigure :loose @15:13:45.81 Forearm.seek :retract @15:13:46.87 Loading da puck from tube 2 into Processing Clamp @15:13:47.11 SC.reconfigure :tight @15:13:47.15 SC.seek 2 @15:13:50.98 SC.configure :limp @15:13:51.20 Elbow.reconfigure :tight @15:13:51.24 Elbow.seek 2 @15:13:53.10 Forearm.reconfigure :tight @15:13:53.15 Forearm.seek 2 + 800 counts @15:13:54.26 SE.reconfigure :pick @15:13:54.32 SE.seek :beamBreak @15:13:57.19 SE.configure :default @15:13:57.35 Elbow.seek 2 @15:13:57.53 Forearm.seek 2 + 300 counts @15:13:57.87 Forearm.reconfigure :loose @15:13:57.92 Hand.close @15:13:59.15 SE.seek :down @15:13:59.35
Forearm.seek :retract @15:14:00.37 Elbow.seek PC @15:14:01.86 SE.stop @15:14:02.94
Elbow.reconfigure :loose @15:14:03.04 Forearm.reconfigure :tight @15:14:03.09 Forearm.seek PC @15:14:04.50 PC.seek :closed @15:14:08.93 Processing Clamp onSpring/offSpring for 12/26 samples, on/off torque 1.55 @15:14:08.94 Hand.open @15:14:09.76 Forearm.reconfigure :loose @15:14:09.80 Forearm.seek :retract @15:14:10.91 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @15:14:10.93 PH.seek 28C,5:00 @15:14:35.71 PH.hold 2:00:00,28C @15:14:35.76 delay 1:30 @15:16:05.80 PSR.select :air @15:16:05.82 PSV.dial PSR @15:16:06.84 Pulling 0.5ml of PSR.wash @15:16:06.85 PSR.select :wash @15:16:06.97 PS.seek 0.5ml @15:16:12.87 delay 5 seconds @15:16:17.89 PSR.select :air @15:16:18.01 PS.seek 2ml @15:16:34.21 PTV.dial :puck @15:16:34.95 PSV.dial PTV @15:16:35.70 PBV.dial :waste1 @15:16:37.12 PS.seek 1.5ml @15:16:43.12 delay 3 seconds @15:16:46.14 PBV.dial between PSV and waste1 @15:16:46.50 PS.seek 0ml @15:17:02.62 delay 5:00 @15:22:02.64 recoverPSRreagent08 @15:22:02.65 PTV.dial :puck @15:22:02.73 PBV.dial :waste1 @15:22:03.10 delay 30 seconds @15:22:33.13 PBV.dial between PSV and waste1 @15:22:33.51 PSR.select :air @15:22:33.52 PSV.dial PSR @15:22:34.39 PS.reconfigure :fast @15:22:34.44 PS.seek 2.5ml @15:22:58.08 PSV.dial PTV @15:22:58.82 delay 5 seconds @15:23:03.84 PSV.dial PSR @15:23:04.57 delay 5 seconds @15:23:09.59 PSV.dial PTV @15:23:10.34 delay 5 seconds @15:23:15.36 PSV.dial :air @15:23:16.12 PS.seek 0ml @15:23:39.49 PTV.dial :PRVmixing,via: PRV @15:23:40.79 PSV.dial :mixloop @15:23:41.26 PRV.dial :ab1 @15:23:42.01 PS.reconfigure :slow @15:23:42.06 PS.seek 0.15ml @15:23:45.58 delay 2 seconds @15:23:47.60 CBV.dial PRV @15:23:47.64
PRV.dial CBV @15:23:48.19 PS.seek 0.34ml @15:23:52.58 delay 2 seconds @15:23:54.60 CBV.dial between PRV and PSV @15:23:54.93 PRV.dial :air @15:23:55.39 PS.seek 0.9ml @15:24:07.40 PRV.dial :waste2 @15:24:08.14 PS.seek 0.5ml @15:24:16.87 addPMLReagent08 @15:24:16.88 PSV.dial :air @15:24:17.78 PS.reconfigure :default @15:24:17.83 PS.seek 2ml @15:24:33.96 PSV.dial :PML @15:24:34.43 PTV.dial :mixing @15:24:35.16 PBV.dial :waste1 @15:24:35.60 PS.seek 1.85ml @15:24:37.99 PBV.dial between PSV and waste1 @15:24:38.37 PS.seek 0ml @15:24:58.05 delay 2 seconds @15:25:00.07 Beginning samplePassC @15:25:00.09 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:25:00.27 Elbow.reconfigure :tight @15:25:00.32 Elbow.seek FlushPuck::Garage @15:25:01.63 Elbow.reconfigure :loose @15:25:01.73 Forearm.seek :garage @15:25:02.84 Hand.close @15:25:03.68 Forearm.seek :retract @15:25:04.70 Elbow.reconfigure :tight @15:25:04.75 Elbow.seek CC @15:25:07.57 Elbow.reconfigure :loose @15:25:07.67 Forearm.reconfigure :tight @15:25:07.72 Forearm.seek CC @15:25:09.01 CC.seek :closed @15:25:12.98 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.04 @15:25:13.05 Hand.open @15:25:13.88 Forearm.reconfigure :loose @15:25:14.30 Forearm.seek :retract @15:25:15.47 PSV.dial PSR @15:25:16.49 Pulling 1.5ml of PSR.flush @15:25:16.50 PSR.select :flush @15:25:16.62 PS.seek 1.5ml @15:25:32.71 delay 5 seconds @15:25:37.73 PSV.dial :waste1 @15:25:38.20 PS.seek 0ml @15:25:54.36 PSV.dial PSR @15:25:54.83 PSR.select :air @15:25:54.95 PS.seek 2.5ml @15:26:21.34 PRV.dial CBV,avoiding: :ab2 @15:26:21.36
CBV.dial PRV @15:26:23.00 PSV.dial :mixloop @15:26:24.04 CTV.dial :air,avoiding: :intake @15:26:24.11 PTV.dial :PRVmixing @15:26:24.87 PS.seek 0ml @15:26:51.17 PSV.dial :air @15:26:51.64 PS.seek 2.5ml @15:27:17.99 PSV.dial :mixloop @15:27:18.46 PS.seek 0ml @15:27:44.83 PSV.dial :air @15:27:45.31 PS.seek 2.5ml @15:28:11.64 PSV.dial :mixloop @15:28:12.12 PS.seek 0ml @15:28:38.46 Garaging FlushPuck in Collection Clamp @15:28:38.53 Elbow.reconfigure :tight @15:28:38.58 Elbow.seek CC @15:28:38.88 Elbow.reconfigure :loose @15:28:38.98 Forearm.seek CC @15:28:39.95 Hand.close @15:28:40.68 CC.seek :open @15:28:44.77 Forearm.seek :retract @15:28:45.78 Elbow.reconfigure :tight @15:28:45.83 Elbow.seek :garage @15:28:48.66 Elbow.reconfigure :loose @15:28:48.76 Forearm.reconfigure :tight @15:28:48.81 Forearm.seek :garage @15:28:50.02 Hand.open @15:28:50.87 Forearm.reconfigure :loose @15:28:50.92 Forearm.seek :retract @15:28:51.98 delayUntil "15:33:00.06" @15:28:52.01 Whole Cell Archive sampling 50ml @15:28:52.03 Loading wcr puck from tube 2 into Collection Clamp @15:28:52.27 SC.reconfigure :tight @15:28:52.32 SC.seek 2 @15:28:52.44 SC.configure :limp @15:28:52.67 Elbow.reconfigure :tight @15:28:52.72 Elbow.seek 2 @15:28:54.61 Forearm.reconfigure :tight @15:28:54.66 Forearm.seek 2 + 800 counts @15:28:55.71 SE.reconfigure :pick @15:28:55.77 SE.seek :beamBreak @15:28:58.66 SE.configure :default @15:28:58.83 Elbow.seek 2 @15:28:59.01 Forearm.seek 2 + 300 counts @15:28:59.18 Forearm.reconfigure :loose @15:28:59.23 Hand.close @15:28:59.95 SE.seek :down @15:29:00.15 Forearm.seek :retract @15:29:01.09 Elbow.seek CC @15:29:02.69 Elbow.reconfigure :loose @15:29:02.80 SE.stop @15:29:02.87 Forearm.reconfigure :tight @15:29:02.92 Forearm.seek CC @15:29:04.69 CC.seek :closed @15:29:08.67 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.99 @15:29:08.68 Hand.open @15:29:09.49 Forearm.reconfigure :loose @15:29:09.54 Forearm.seek :retract @15:29:10.56 collectSample 50ml w/bubble point of 35psi @15:29:10.58 Priming 7ml @15:29:10.59 Intake.select :close @15:29:10.60 Exhaust.select :close @15:29:10.61 SS.hold @15:29:10.82 Calibrating pressure sensors @15:29:10.83 CTV.dial :air @15:29:10.91 SSV.dial :spit,avoiding: :exhaust @15:29:10.93 SS.reconfigure :fast @15:29:10.98 SS.seek 5ml @15:29:13.78 SSV.dial CBV @15:29:13.81 CBV.dial SSV @15:29:15.18 delay 3.8 seconds @15:29:19.12 Sensor limits: Intake max 86.4psia; Outlet max 105.0psia @15:29:19.13 SSV.dial :spit @15:29:20.46 SS.seek 0ml @15:29:23.28 SSV.dial CBV @15:29:24.61 CTV.dial :intake @15:29:25.36 SSV.dial CBV @15:29:25.39 CBV.dial SSV @15:29:25.58 Allowing 40s for 7.0ml of remaining 7.0ml @15:29:25.59 Intake.select :open @15:29:27.76 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:29:27.78 SS.reconfigure :inhaling 2.17ml/s @15:29:27.83 SS.seek 7ml @15:29:33.88 Stroke completed -- waiting for pressure to equalize ... @15:29:33.90 SS.reconfigure :exhale @15:29:33.94 SS.hold @15:29:37.80 Intake.select :close @15:29:37.96 SSV.dial :exhaust @15:29:38.73 Exhaust.select :open @15:29:38.85 SS.seek 0ml @15:29:42.84 Exhaust.select :close @15:29:42.95 Sampling 50.0ml @15:29:42.96 Intake.select :close @15:29:42.98 Exhaust.select :close @15:29:42.99 SS.reconfigure :default @15:29:43.03 SS.hold @15:29:43.23 CTV.dial :intake @15:29:43.25 SSV.dial CBV @15:29:43.28 CBV.dial SSV @15:29:44.10 Allowing 130s for 25.0ml of remaining 50.0ml @15:29:44.11 Intake.select :open @15:29:46.28 Intake @14.0psia, vacuum from 8.5 to 11.5psi @15:29:46.30 SS.reconfigure :inhaling 2.17ml/s @15:29:46.34 SS.seek 25ml @15:30:01.65 Stroke completed -- waiting for pressure to equalize ... @15:30:01.66 SS.reconfigure :exhale @15:30:01.71 SS.hold @15:30:12.90 Intake.select :close @15:30:13.06 SSV.dial :exhaust @15:30:13.84 Exhaust.select :open @15:30:13.97 SS.seek 0ml @15:30:23.20 Exhaust.select :close @15:30:23.31 SSV.dial CBV @15:30:23.34 CBV.dial SSV @15:30:24.12 Allowing 130s for 25.0ml of remaining 25.0ml @15:30:24.13 Intake.select :open @15:30:26.30 Intake @14.2psia, vacuum from 8.7 to 11.7psi @15:30:26.32 SS.reconfigure :inhaling 2.17ml/s @15:30:26.37 SS.seek 25ml @15:30:41.60 Stroke completed -- waiting for pressure to equalize ... @15:30:41.61 SS.reconfigure :exhale @15:30:41.66 SS.hold @15:30:53.29 Intake.select :close @15:30:53.45 SSV.dial :exhaust @15:30:54.21 Exhaust.select :open @15:30:54.33 SS.seek 0ml @15:31:03.70 Exhaust.select :close @15:31:03.81 Sampled 50.0ml @15:31:03.83 Drying puck before fixing for 4:00 @15:31:03.84 CBV.dial :waste2b @15:31:04.60 delay 1:00 @15:32:04.62 CSV.dial :air @15:32:05.08 CS.reconfigure :fast @15:32:05.14 CS.seek 10ml @15:32:28.94 Pumping wcr puck from tube 2 up to 35psi... @15:32:28.96 CBV.dial SSV @15:32:30.22 CBV.dial between CSV and waste2b @15:32:31.20 CSV.dial between air and CTV @15:32:31.52 CS.seek 7.248ml @15:32:38.63 CTV.dial CSV,avoiding: :air @15:32:38.65 CSV.dial CTV @15:32:39.47 CS.reconfigure :pressurize @15:32:39.53 CS.seek 0ml @15:32:52.11 CBV.dial :waste2b @15:32:52.44 CS.reconfigure :default @15:32:52.49 CS.seek 0ml @15:32:59.69 delay 1:00 @15:33:00.07 recoverPRVreagent08 @15:33:00.08 PTV.dial :mixing @15:33:00.83 PBV.dial :waste1 @15:33:01.20 delay 30 seconds @15:33:31.23 PBV.dial between PSV and waste1 @15:33:31.74 PSV.dial :air @15:33:32.21 PS.reconfigure :fast @15:33:32.26 PS.seek 2.5ml @15:33:55.76 PSV.dial :mixloop @15:33:56.23 delay 2 seconds @15:33:58.25 PSV.dial :air @15:33:58.72 delay 2 seconds @15:33:59.71 CTV.dial :air,avoiding: :intake @15:34:00.74 PSV.dial :mixloop @15:34:01.07 Fix #1 of 1 (with RNAlater) for 4:00 @15:34:01.08 CSV.dial CSR @15:34:01.20 delay 2 seconds @15:34:01.51 Pulling 2ml of CSR.RNAlater @15:34:01.53 CSR.select :RNAlater @15:34:01.65 CS.seek 2ml @15:34:03.22 PSV.dial :air @15:34:03.69 PS.seek 0ml @15:34:07.65 delay 5 seconds @15:34:12.68 CSR.select :air @15:34:12.79 CS.reconfigure :fast @15:34:12.84 CS.seek 10ml @15:34:27.14 washCycleDA #1 of 2 @15:34:27.15 PSR.select :air @15:34:27.17 PSV.dial PSR @15:34:28.48 Pulling 0.5ml of PSR.wash @15:34:28.49 PSR.select :wash @15:34:28.61 PS.reconfigure :default @15:34:28.66 PS.seek 0.5ml @15:34:32.00 Pumping wcr puck from tube 2 up to 35psi... @15:34:32.02 CBV.dial SSV @15:34:33.27 CBV.dial between CSV and waste2b @15:34:34.25 CSV.dial between air and CTV @15:34:34.61 delay 5 seconds @15:34:34.85 CS.seek 7.077ml @15:34:39.62 PSR.select :air @15:34:39.74 PS.seek 2ml @15:34:42.38 CTV.dial CSV,avoiding: :air @15:34:42.40 CSV.dial CTV @15:34:43.80 CS.reconfigure :pressurize @15:34:43.85 CS.seek 0ml @15:34:55.98 PTV.dial :puck @15:34:56.72 PSV.dial PTV @15:34:57.47 PBV.dial :waste1 @15:34:57.87 delay 4:00 @15:34:57.97 PS.seek 1.5ml @15:35:03.86 delay 3 seconds @15:35:06.88 PBV.dial between PSV and waste1 @15:35:07.27 PS.seek 0ml @15:35:23.42 delay 2:00 @15:37:23.46 recoverPSRreagent08 @15:37:23.47 PTV.dial :puck @15:37:23.54 PBV.dial :waste1 @15:37:23.94 delay 30 seconds @15:37:53.96 PBV.dial between PSV and waste1 @15:37:54.35 PSR.select :air @15:37:54.37 PSV.dial PSR @15:37:55.13 PS.reconfigure :fast @15:37:55.18 PS.seek 2.5ml @15:38:18.70 PSV.dial PTV @15:38:19.45 delay 5 seconds @15:38:24.47 PSV.dial PSR @15:38:25.21 delay 5 seconds @15:38:30.24 PSV.dial PTV @15:38:31.00 delay 5 seconds @15:38:36.01 PSV.dial :air @15:38:36.78 PS.seek 0ml @15:38:57.90 CBV.dial :waste2b @15:38:58.23 CS.reconfigure :default @15:38:58.28 CS.seek 0ml @15:39:00.23 washCycleDA #2 of 2 @15:39:00.24 PSR.select :air @15:39:00.26 PSV.dial PSR @15:39:01.57 Pulling 0.5ml of PSR.wash @15:39:01.58 PSR.select :wash @15:39:01.70 PS.reconfigure :default @15:39:01.75 PS.seek 0.5ml @15:39:03.81 delay 1:00 @15:39:07.88 delay 5 seconds @15:39:12.91 PSR.select :air @15:39:13.02 PS.seek 2ml @15:39:29.16 PTV.dial :puck @15:39:29.24 PSV.dial PTV @15:39:29.98 PBV.dial :waste1 @15:39:30.43 PS.seek 1.5ml @15:39:36.45 delay 3 seconds @15:39:39.47 PBV.dial between PSV and waste1 @15:39:39.86 PS.seek 0ml @15:39:55.95 delay 2:00 @15:40:03.83 CTV.dial :air,avoiding: :intake @15:41:55.97 recoverPSRreagent08 @15:41:55.98 PTV.dial :puck @15:41:56.05 PBV.dial :waste1 @15:41:56.43 delay 30 seconds @15:42:26.46 PBV.dial between PSV and waste1 @15:42:26.83 PSR.select :air @15:42:26.85 PSV.dial PSR @15:42:27.61 PS.reconfigure :fast @15:42:27.66 PS.seek 2.5ml @15:42:51.16 PSV.dial PTV @15:42:51.91 delay 5 seconds @15:42:56.93 PSV.dial PSR @15:42:57.79 delay 5 seconds @15:43:02.81 PSV.dial PTV @15:43:03.54 delay 5 seconds @15:43:08.56 PSV.dial :air @15:43:09.33 PS.seek 0ml @15:43:32.82 getPMLReagent08 @15:43:32.83 PRV.dial :air @15:43:33.29 delay 5 seconds @15:43:38.31 PTV.dial :PRVmixing @15:43:40.07 delay 5 seconds @15:43:45.09 PRV.dial :ab2 @15:43:45.54 PTV.dial :PRVmixing @15:43:45.61 PSV.dial :mixloop @15:43:46.09 PS.reconfigure :default @15:43:46.14 PS.seek 0.3ml @15:43:50.10 delay 2 seconds @15:43:52.12 PRV.dial :air @15:43:52.63 PS.seek 0.55ml @15:43:55.94 addPMLReagent08 @15:43:55.96 PSV.dial :air @15:43:56.42 PS.seek 2ml @15:44:11.97 PSV.dial :PML @15:44:12.44 PTV.dial :mixing @15:44:13.18 PBV.dial :waste1 @15:44:13.61 PS.seek 1.85ml @15:44:15.97 PBV.dial between PSV and waste1 @15:44:16.36 PS.seek 0ml @15:44:36.26 delay 2 seconds @15:44:38.28 delay 8:00 @15:52:38.30 recoverPRVreagent08 @15:52:38.31 PTV.dial :mixing @15:52:38.39 PBV.dial :waste1 @15:52:38.78 delay 30 seconds @15:53:08.81 PBV.dial between PSV and waste1 @15:53:09.20 PSV.dial :air @15:53:09.68 PS.reconfigure :fast @15:53:09.72 PS.seek 2.5ml @15:53:33.47 PSV.dial :mixloop @15:53:33.94 delay 2 seconds @15:53:35.95 PSV.dial :air @15:53:36.42 delay 2 seconds @15:53:38.44 PSV.dial :mixloop @15:53:38.91 delay 2 seconds @15:53:40.92 PSV.dial :air @15:53:41.39 PS.seek 0ml @15:54:04.88 washCycleDA #1 of 3 @15:54:04.89 PSR.select :air @15:54:04.90 PSV.dial PSR @15:54:06.23 Pulling 0.5ml of PSR.wash @15:54:06.24 PSR.select :wash @15:54:06.36 PS.reconfigure :default @15:54:06.41 PS.seek 0.5ml @15:54:12.40 delay 5 seconds @15:54:17.42 PSR.select :air @15:54:17.54 PS.seek 2ml @15:54:33.75 PTV.dial :puck @15:54:34.48 PSV.dial PTV @15:54:35.22 PBV.dial :waste1 @15:54:35.67 PS.seek 1.5ml @15:54:41.65 delay 3 seconds @15:54:44.67 PBV.dial between PSV and waste1 @15:54:45.07 PS.seek 0ml @15:55:01.22 delay 2:00 @15:57:01.25 recoverPSRreagent08 @15:57:01.26 PTV.dial :puck @15:57:01.32 PBV.dial :waste1 @15:57:01.72 delay 30 seconds @15:57:31.74 PBV.dial between PSV and waste1 @15:57:32.26 PSR.select :air @15:57:32.28 PSV.dial PSR @15:57:33.14 PS.reconfigure :fast @15:57:33.19 PS.seek 2.5ml @15:57:56.93 PSV.dial PTV @15:57:57.67 delay 5 seconds @15:58:02.69 PSV.dial PSR @15:58:03.54 delay 5 seconds @15:58:08.56 PSV.dial PTV @15:58:09.29 delay 5 seconds @15:58:14.31 PSV.dial :air @15:58:15.10 PS.seek 0ml @15:58:38.62 washCycleDA #2 of 3 @15:58:38.63 PSR.select :air @15:58:38.65 PSV.dial PSR @15:58:39.96 Pulling 0.5ml of PSR.wash @15:58:39.97 PSR.select :wash @15:58:40.09 PS.reconfigure :default @15:58:40.14 PS.seek 0.5ml @15:58:46.15 delay 5 seconds @15:58:51.17 PSR.select :air @15:58:51.29 PS.seek 2ml @15:59:07.40 PTV.dial :puck @15:59:07.41 PSV.dial PTV @15:59:08.15 PBV.dial :waste1 @15:59:08.59 PS.seek 1.5ml @15:59:14.59 delay 3 seconds @15:59:17.61 PBV.dial between PSV and waste1 @15:59:17.99 PS.seek 0ml @15:59:34.09 delay 2:00 @16:01:34.12 recoverPSRreagent08 @16:01:34.13 PTV.dial :puck @16:01:34.14 PBV.dial :waste1 @16:01:34.51 delay 30 seconds @16:02:04.54 PBV.dial between PSV and waste1 @16:02:04.91 PSR.select :air @16:02:04.93 PSV.dial PSR @16:02:05.69 PS.reconfigure :fast @16:02:05.74 PS.seek 2.5ml @16:02:29.43 PSV.dial PTV @16:02:30.18 delay 5 seconds @16:02:35.20 PSV.dial PSR @16:02:35.96 delay 5 seconds @16:02:40.98 PSV.dial PTV @16:02:41.73 delay 5 seconds @16:02:46.75 PSV.dial :air @16:02:47.51 PS.seek 0ml @16:03:11.03 washCycleDA #3 of 3 @16:03:11.04 PSR.select :air @16:03:11.05 PSV.dial PSR @16:03:12.37 Pulling 0.5ml of PSR.wash @16:03:12.38 PSR.select :wash @16:03:12.50 PS.reconfigure :default @16:03:12.54 PS.seek 0.5ml @16:03:18.46 delay 5 seconds @16:03:23.48 PSR.select :air @16:03:23.60 PS.seek 2ml @16:03:39.71 PTV.dial :puck @16:03:39.72 PSV.dial PTV @16:03:40.46 PBV.dial :waste1 @16:03:40.89 PS.seek 1.5ml @16:03:46.90 delay 3 seconds @16:03:49.92 PBV.dial between PSV and waste1 @16:03:50.29 PS.seek 0ml @16:04:06.49 delay 2:00 @16:06:06.52 recoverPSRreagent08 @16:06:06.53 PTV.dial :puck @16:06:06.54 PBV.dial :waste1 @16:06:06.93 delay 30 seconds @16:06:36.95 PBV.dial between PSV and waste1 @16:06:37.35 PSR.select :air @16:06:37.37 PSV.dial PSR @16:06:38.12 PS.reconfigure :fast @16:06:38.17 PS.seek 2.5ml @16:07:01.92 PSV.dial PTV @16:07:02.68 delay 5 seconds @16:07:07.70 PSV.dial PSR @16:07:08.44 delay 5 seconds @16:07:13.46 PSV.dial PTV @16:07:14.21 delay 5 seconds @16:07:19.23 PSV.dial :air @16:07:20.01 PS.seek 0ml @16:07:43.58 Gate.power :camera,:ON @16:07:43.60 Mixing DA Substrates PSR.select :air @16:07:43.62 PSV.dial PSR @16:07:44.99 Pulling 0.25ml of PSR.sub1 @16:07:45.01 PSR.select :sub1 @16:07:45.23 PS.reconfigure :default @16:07:45.36 PS.seek 0.25ml @16:07:48.78 delay 5 seconds @16:07:53.80 Pulling 0.25ml of PSR.sub2 @16:07:53.81 PSR.select :sub2 @16:07:53.93 PS.seek 0.5ml @16:07:57.33 delay 5 seconds @16:08:02.35 PSR.select :air @16:08:02.47 PS.seek 2ml @16:08:18.55 PTV.dial :puck @16:08:18.56 PSV.dial PTV @16:08:19.31 PBV.dial :waste1 @16:08:19.76 PS.seek 1.5ml @16:08:25.74 delay 3 seconds @16:08:28.76 PBV.dial between PSV and waste1 @16:08:29.15 PS.seek 0ml @16:08:45.27 delay 10 seconds @16:08:55.30 recoverPSRreagent08 @16:08:55.31 PTV.dial :puck @16:08:55.32 PBV.dial :waste1 @16:08:55.69 delay 30 seconds @16:09:25.72 PBV.dial between PSV and waste1 @16:09:26.10 PSR.select :air @16:09:26.11 PSV.dial PSR @16:09:26.87 PS.reconfigure :fast @16:09:26.92 PS.seek 2.5ml @16:09:50.83 PSV.dial PTV @16:09:51.58 delay 5 seconds @16:09:56.60 PSV.dial PSR @16:09:57.35 delay 5 seconds @16:10:02.37 PSV.dial PTV @16:10:03.11 delay 5 seconds @16:10:08.13 PSV.dial :air @16:10:08.91 PS.seek 0ml @16:10:32.71 Additional air clear @16:10:32.72 PSR.select :air @16:10:32.73 PSV.dial PSR @16:10:34.06 Pulling 2ml of PSR.air @16:10:34.07 PSR.select :air @16:10:34.10 PS.reconfigure :default @16:10:34.14 PS.seek 2ml @16:10:55.45 delay 5 seconds @16:11:00.47 PTV.dial :puck @16:11:00.49 PSV.dial PTV @16:11:01.23 PBV.dial :waste1 @16:11:01.67 PS.seek 1.5ml @16:11:07.73 delay 3 seconds @16:11:10.75 PBV.dial between PSV and waste1 @16:11:11.13 delay 1 second @16:11:12.15 PSV.dial :waste1 @16:11:13.20 PS.seek 0ml @16:11:29.39 Processing Puck Heater at 28C before imaging @16:11:29.40 PH.coast @16:11:29.51 Imaging da puck in Processing Clamp while storing it to tube 1 @16:11:29.69 SC.reconfigure :tight @16:11:29.74 SC.seek 1 @16:11:33.30 SC.configure :limp @16:11:33.52 Elbow.reconfigure :tight @16:11:33.57 Elbow.seek 1 @16:11:35.25 Forearm.reconfigure :tight @16:11:35.30 Forearm.seek 1 + 800 counts @16:11:36.40 SE.reconfigure :pick @16:11:36.46 SE.seek :beamBreak @16:11:39.30 SE.configure :default @16:11:39.47 Elbow.reconfigure :limp @16:11:39.52 Forearm.reconfigure :loose @16:11:39.56 Forearm.seek 1 @16:11:39.96 Elbow.hold @16:11:40.03 Elbow.reconfigure :tight @16:11:40.13 SE.seek 1.1 Pucks below Top Plate @16:11:40.59 Forearm.force -20 @16:11:40.63 Forearm.seek 1 + 320 counts @16:11:41.00 Forearm.reconfigure :tight @16:11:41.05 Forearm.seek 1 + 800 counts @16:11:41.49 SE.seek 2.1 Pucks below Top Plate @16:11:42.05 Forearm.reconfigure :loose @16:11:42.10 Forearm.seek :retract @16:11:43.03 Elbow.seek PC @16:11:45.55 Elbow.reconfigure :loose @16:11:45.65 Forearm.seek PC @16:11:46.82 Hand.close @16:11:47.52 PC.seek :open @16:11:52.07 Forearm.seek :retract @16:11:53.17 Elbow.reconfigure :tight @16:11:53.22 Elbow.seek 1 @16:11:55.88 Forearm.reconfigure :tight @16:11:55.93 Forearm.seek 1 + 800 counts @16:11:56.82 Hand.open @16:11:57.57 SE.seek 1.86 Pucks below Top Plate @16:11:57.84 SE.reconfigure :seal @16:11:57.89 SE.seek 1.2 Pucks below Top Plate @16:11:58.41 Lights, camera, ACTION! @16:11:58.45 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da25sep0216h50ml.tif @16:12:17.94 Waiting >20 seconds for da to relinquish FlushPuck @16:12:27.15 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 25.144 seconds /var/log/esp/da25sep0216h50ml.tif: TIFF Upload Complete @16:12:27.18 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da25sep0216h50ml15s.tif @16:12:42.79 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da25sep0216h50ml15s.tif: TIFF Upload Complete @16:12:42.81 PuckCamera: snap -bin=2,2 22.0 /var/log/esp/midres/da25sep0216h50ml22s.tif @16:13:05.43 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 22 seconds /var/log/esp/midres/da25sep0216h50ml22s.tif: TIFF Upload Complete @16:13:05.45 DAfans.email "25.144 sec auto-exposure",Subject: "da25sep0216h50ml images!" @16:13:05.48 Gate.power :camera,:OFF @16:13:05.89 Elbow.seek 1 @16:13:06.17 Forearm.seek 1 + 800 counts @16:13:06.25 SE.reconfigure :gently @16:13:06.32 SE.seek 1.91 Pucks below Top Plate @16:13:07.06 Elbow.reconfigure :limp @16:13:07.12 Forearm.reconfigure :loose @16:13:07.17 Forearm.seek 1 @16:13:07.62 Elbow.hold @16:13:07.69 Elbow.reconfigure :tight @16:13:07.74 SE.reconfigure :default @16:13:07.80 SE.seek 2.1 Pucks below Top Plate @16:13:08.19 Forearm.force -20 @16:13:08.23 Forearm.seek 1 + 308 counts @16:13:09.40 Forearm.reconfigure :tight @16:13:09.46 Forearm.seek 1 + 800 counts @16:13:09.95 SE.seek :down @16:13:11.21 Forearm.reconfigure :loose @16:13:11.31 Forearm.seek :retract @16:13:12.53 SE.stop @16:13:12.74 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:13:13.28 Elbow.seek FlushPuck::Garage @16:13:15.28 Elbow.reconfigure :loose @16:13:15.50 Forearm.seek :garage @16:13:16.46 Hand.close @16:13:17.52 Forearm.seek :retract @16:13:18.55 Elbow.reconfigure :tight @16:13:18.65 Elbow.seek PC @16:13:19.98 Elbow.reconfigure :loose @16:13:20.20 Forearm.reconfigure :tight @16:13:20.28 Forearm.seek PC @16:13:22.01 PC.seek :closed @16:13:26.57 Processing Clamp onSpring/offSpring for 12/27 samples, on/off torque 1.59 @16:13:26.71 Hand.open @16:13:27.66 Forearm.reconfigure :loose @16:13:27.75 Forearm.seek :retract @16:13:28.93 PSR.select :air @16:13:29.00 Storing wcr puck in Collection Clamp to tube 1 @16:13:29.04 PSV.dial PSR @16:13:30.40 Pulling 0.5ml of PSR.flush @16:13:30.46 PSR.select :flush @16:13:30.95 PS.seek 0.5ml @16:13:31.34 Elbow.reconfigure :tight @16:13:31.43 Elbow.seek 1 @16:13:34.29 Forearm.reconfigure :tight @16:13:34.39 Forearm.seek 1 + 800 counts @16:13:35.55 SE.reconfigure :pick @16:13:35.66 SE.seek :beamBreak @16:13:36.96 delay 5 seconds @16:13:38.57 SE.configure :default @16:13:38.89 Elbow.reconfigure :limp @16:13:39.00 Forearm.reconfigure :loose @16:13:39.09 Forearm.seek 1 @16:13:39.53 Elbow.hold @16:13:39.67 Elbow.reconfigure :tight @16:13:39.88 SE.seek 2.1 Pucks below Top Plate @16:13:40.49 Forearm.force -20 @16:13:40.58 Forearm.seek 1 + 319 counts @16:13:41.16 Forearm.reconfigure :tight @16:13:41.26 Forearm.seek 1 + 800 counts @16:13:41.62 SE.seek 3.1 Pucks below Top Plate @16:13:41.99 PSR.select :air @16:13:42.34 Forearm.reconfigure :loose @16:13:42.40 Forearm.seek :retract @16:13:42.42 PS.seek 2.5ml @16:13:42.83 upsync @16:13:43.47 Elbow.seek CC @16:13:45.48 Elbow.reconfigure :loose @16:13:46.15 Forearm.seek CC @16:13:47.17 Hand.close @16:13:47.89 CC.seek :open @16:13:52.11 Forearm.seek :retract @16:13:53.15 Elbow.reconfigure :tight @16:13:53.21 Elbow.seek 1 @16:13:54.89 Elbow.reconfigure :limp @16:13:54.95 Forearm.seek 1 @16:13:56.09 Elbow.hold @16:13:56.15 Elbow.reconfigure :tight @16:13:56.20 Hand.open @16:13:57.01 Forearm.force -20 @16:13:57.06 Forearm.seek 1 + 320 counts @16:13:57.43 Forearm.reconfigure :tight @16:13:57.48 Forearm.seek 1 + 800 counts @16:13:57.86 SE.seek :down @16:13:58.99 Forearm.reconfigure :loose @16:13:59.04 Forearm.seek :retract @16:14:00.28 SE.stop @16:14:00.33
Starting finalPurge @16:14:03.77 delay 1 second @16:14:04.78 PRV.dial :waste2,avoiding: :ab2 @16:14:06.65 PTV.dial :PRVmixing @16:14:07.95 PSV.dial :mixloop @16:14:08.99 PS.seek :empty @16:14:20.29
Waiting >20 seconds for psFlush to relinquish FlushPuck @16:14:35.39 PSR.select :air @16:14:35.41 PSV.dial PSR @16:14:36.44 Pulling 0.5ml of PSR.flush @16:14:36.45 PSR.select :flush @16:14:37.01 PS.seek 0.5ml @16:14:43.14 delay 5 seconds @16:14:48.16 PSR.select :air @16:14:48.28 PS.seek 2.5ml @16:15:09.61 delay 1 second @16:15:10.63 PBV.dial :waste1 @16:15:11.00 PTV.dial :mixing @16:15:11.73 PSV.dial :mixloop @16:15:12.78 PS.seek :empty @16:15:39.17 PSV.dial PSR @16:15:40.20 PSR.select :flush @16:15:40.33 PS.seek 1ml @16:15:51.42 PSR.select :air @16:15:51.53 PS.seek 2.5ml @16:16:07.76 PSV.dial PTV @16:16:08.51 PTV.dial :puck @16:16:09.25 PBV.dial :waste1 @16:16:09.33 PS.seek 0ml @16:16:35.89 PSV.dial PSR @16:16:36.64 PSR.select :flush @16:16:36.76 PS.seek 0.5ml @16:16:42.67 PSR.select :air @16:16:42.78 PS.seek 2.5ml @16:17:04.01 PSV.dial PTV @16:17:04.76 PBV.dial PSV @16:17:05.35 PS.seek 0ml @16:17:31.67 PSV.dial PBV @16:17:32.13 PS.seek 2.5ml @16:17:58.57 PSV.dial :waste1 @16:17:59.31 PS.seek 0ml @16:18:25.67 PSV.dial PSR @16:18:26.14 PSR.select :air @16:18:26.16 PS.seek 2.5ml @16:18:52.54 PSV.dial PTV @16:18:53.28 PBV.dial PSV @16:18:53.36 PS.seek 0ml @16:19:19.73 PSV.dial PBV @16:19:20.21 PS.seek 2.5ml @16:19:46.60 PSV.dial :waste1 @16:19:47.34 PS.seek 0ml @16:20:13.85 PSV.dial PSR @16:20:14.32 PSR.select :flush @16:20:14.45 PS.seek 1ml @16:20:25.41 PSR.select :air @16:20:25.92 PS.seek 2.5ml @16:20:42.10 PSV.dial PTV @16:20:42.84 PTV.dial :puck @16:20:42.92 PBV.dial :waste1 @16:20:43.50 PS.seek 0ml @16:21:09.79 PSV.dial PSR @16:21:10.52 PSR.select :flush @16:21:10.64 PS.seek 0.5ml @16:21:16.54 PSR.select :air @16:21:16.66 PS.seek 2.5ml @16:21:37.91 PSV.dial PTV @16:21:38.67 PBV.dial PSV @16:21:39.25 PS.seek 0ml @16:22:05.57 PSV.dial PBV @16:22:06.16 PS.seek 2.5ml @16:22:32.54 PSV.dial :waste1 @16:22:33.30 PS.seek 0ml @16:22:59.63 PSV.dial PSR @16:23:00.10 PSR.select :air @16:23:00.13 PS.seek 2.5ml @16:23:26.44 PSV.dial PTV @16:23:27.18 PBV.dial PSV @16:23:27.37 PS.seek 0ml @16:23:53.73 PSV.dial PBV @16:23:54.19 PS.seek 2.5ml @16:24:20.54 PSV.dial :waste1 @16:24:21.28 PS.seek 0ml @16:24:47.70
Loading FlushPuck into Collection Clamp from Processing Clamp @16:24:47.89 Elbow.seek PC @16:24:50.40 Elbow.reconfigure :loose @16:24:50.50 Forearm.seek PC @16:24:51.61 Hand.close @16:24:52.38 PC.seek :open @16:24:56.92 Forearm.seek :retract @16:24:57.94 Elbow.reconfigure :tight @16:24:57.99 Elbow.seek CC @16:25:01.73 Elbow.reconfigure :loose @16:25:01.84 Forearm.reconfigure :tight @16:25:02.78 Forearm.seek CC @16:25:04.14 CC.seek :closed @16:25:08.12 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.03 @16:25:08.19 Hand.open @16:25:08.93 Forearm.reconfigure :loose @16:25:08.98 Forearm.seek :retract @16:25:10.08 Flushing the Collection Valve::Manifold @16:25:10.20 CSV.dial CSR @16:25:10.64 Pulling 5ml of CSR.flush @16:25:10.65 CSR.select :flush @16:25:10.77 CS.seek 5ml @16:25:24.33 delay 5 seconds @16:25:29.35 CSR.select :air @16:25:29.51 CS.reconfigure :fast @16:25:29.56 CS.seek 7.5ml @16:25:36.17 CSV.dial :waste @16:25:37.44 CS.reconfigure :default @16:25:37.49 CS.seek 0ml @16:25:57.52 Flushing lower manifold's waste1 port @16:25:57.65 CSV.dial :flush @16:25:58.18 CS.reconfigure :fast @16:25:58.37 CS.seek 5ml @16:26:10.77 delay 5 seconds @16:26:15.79 CSV.dial :air @16:26:16.30 CS.seek 7ml @16:26:21.68 CSV.dial CTV @16:26:21.70
CTV.dial CSV @16:26:23.09 CBV.dial :waste1 @16:26:23.87 CS.seek 0ml @16:26:40.58 Flushing lower manifold's waste2 port @16:26:40.70 CSV.dial :flush @16:26:41.43 CS.seek 5ml @16:26:53.70 delay 5 seconds @16:26:58.72 CSV.dial :air @16:26:59.23 CS.seek 7ml @16:27:05.11 CSV.dial CTV @16:27:05.14
CTV.dial CSV @16:27:05.59 CBV.dial :waste2 @16:27:06.65 CS.seek 0ml @16:27:23.30 Flushing lower manifold's waste2b port @16:27:23.42 CSV.dial :flush @16:27:24.16 CS.seek 5ml @16:27:36.39 delay 5 seconds @16:27:41.41 CSV.dial :air @16:27:41.94 CS.seek 7ml @16:27:47.37 CSV.dial CTV @16:27:47.39
CTV.dial CSV @16:27:47.84 CBV.dial :waste2b @16:27:48.88 CS.seek 0ml @16:28:05.49 Disinfecting external Intake with kill @16:28:05.61 CSV.dial CSR @16:28:06.05 Pulling 8ml of CSR.kill @16:28:06.06 CSR.select :kill @16:28:06.18 CS.seek 8ml @16:28:25.30 delay 5 seconds @16:28:30.32 CSR.select :air @16:28:30.43 CS.seek 10ml @16:28:35.86 CSV.dial CBV @16:28:35.88
CBV.dial CSV @16:28:36.94 CTV.dial :intake @16:28:37.70 Intake.select :open @16:28:37.82 CS.seek 2ml @16:28:56.76 Intake.select :close @16:28:56.87 CTV.dial :air @16:28:57.61 CS.reconfigure :default @16:28:57.66 CS.seek 0ml @16:29:03.54 CSV.dial CSR @16:29:04.55 Pulling 5ml of CSR.flush CSR.select :flush @16:29:04.67 CS.seek 5ml @16:29:18.33 delay 5 seconds @16:29:23.35 CSR.select :air @16:29:23.46 CS.seek 10ml @16:29:37.04 CSV.dial CBV @16:29:38.05 CS.seek 0ml @16:30:04.42 Garaging FlushPuck in Collection Clamp @16:30:04.49 Elbow.reconfigure :tight @16:30:04.54 Elbow.seek CC @16:30:04.85 Elbow.reconfigure :loose @16:30:04.95 Forearm.seek CC @16:30:05.87 Hand.close @16:30:06.62 CC.seek :open @16:30:11.16 Forearm.seek :retract @16:30:12.15 Elbow.reconfigure :tight @16:30:12.20 Elbow.seek :garage @16:30:14.99 Elbow.reconfigure :loose @16:30:15.09 Forearm.reconfigure :tight @16:30:15.14 Forearm.seek :garage @16:30:16.30 Hand.open @16:30:17.05 Forearm.reconfigure :loose @16:30:17.10 Forearm.seek :retract @16:30:18.10 Completed [initialPurge; da [50, 50] #da and wsr are run simultanelously] begun 14:39:22 @16:30:18.15 Asleep after completing DA_WCR_110124 assays @16:30:18.17 GoodNews.email "{1 => 4,\n 2 => 0}\nCTD data unavailable\nCan@16:28:36, 40% flow, 26.2C, 38% humidity, 14.9psia, 13.47V, 0.730A avg, 1.071Ah, 9.83W\nSampled 50.0ml",Subject: "Asleep after completing DA_WCR_110124 assays" @16:30:22.50 upsync idity, 14.7psia, 13.72V, 0.284A avg, 29.036Ah, 3.89W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:29:43.74
SC.reconfigure :tight @13:29:43.81 SC.seek 2 @13:29:47.48 upsync @13:29:50.66
SC.configure :limp @13:29:50.94 Elbow.reconfigure :tight @13:29:51.05 Elbow.seek 2 @13:29:52.94 Forearm.reconfigure :tight @13:29:52.99 Forearm.seek 2 + 800 counts @13:29:54.06 SE.reconfigure :pick @13:29:54.13 SE.seek :beamBreak @13:29:55.89 SE.configure :default @13:29:56.05 Elbow.seek 2 @13:29:56.24 Forearm.seek 2 + 300 counts @13:29:56.68 Forearm.reconfigure :loose @13:29:56.73 Hand.close @13:29:57.45 SE.seek :down @13:29:57.66
Forearm.seek :retract @13:29:58.68 Elbow.seek CC @13:29:58.98 SE.stop @13:30:00.32
Elbow.reconfigure :loose @13:30:00.42 Forearm.reconfigure :tight @13:30:00.47 Forearm.seek CC @13:30:01.77 CC.seek :closed @13:30:05.76 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.03 @13:30:05.77 Hand.open @13:30:06.62 Forearm.reconfigure :loose @13:30:06.67 Forearm.seek :retract @13:30:07.64 collectSample 1000ml w/bubble point of 24psi @13:30:07.65 Priming 7ml @13:30:07.66 Intake.select :close @13:30:07.67 Exhaust.select :close @13:30:07.68 SS.hold @13:30:07.91 Calibrating pressure and temperature sensors @13:30:07.93 CTV.dial :air @13:30:07.95 SSV.dial :spit,avoiding: :exhaust @13:30:07.97 SS.reconfigure :fast @13:30:08.02 SS.seek 5ml @13:30:10.88 SSV.dial CBV @13:30:10.92
CBV.dial SSV @13:30:12.26 delay 3.8 seconds @13:30:16.18 Sensor limits: Intake max 110.3psia; Outlet max 114.9psia @13:30:16.19 SSV.dial :spit @13:30:17.53 SS.seek 0ml @13:30:20.25 SSV.dial CBV @13:30:21.60 CTV.dial :intake @13:30:23.23 SSV.dial CBV @13:30:23.27
CBV.dial SSV @13:30:23.49 Allowing 40s for 7.0ml of remaining 7.0ml @13:30:23.50 Intake.select :open @13:30:25.66 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:30:25.68 SS.reconfigure :inhaling 2.17ml/s @13:30:25.73 SS.seek 7ml @13:30:32.28 Stroke completed -- waiting for pressure to equalize ... @13:30:32.29 SS.reconfigure :exhale @13:30:32.34 SS.hold @13:30:33.86 Intake.select :close @13:30:34.44 SSV.dial :exhaust @13:30:35.22 Exhaust.select :open @13:30:35.33 SS.seek 0ml @13:30:39.58 Exhaust.select :close @13:30:39.69 Sampling 1000.0ml @13:30:39.70 Intake.select :close @13:30:39.71 Exhaust.select :close @13:30:39.72 SS.reconfigure :default @13:30:39.77 SS.hold @13:30:39.98 CTV.dial :intake @13:30:40.00 SSV.dial CBV @13:30:40.02
CBV.dial SSV @13:30:40.80 Allowing 130s for 25.0ml of remaining 1000.0ml @13:30:40.81 Intake.select :open @13:30:42.97 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:30:42.99 SS.reconfigure :inhaling 2.17ml/s @13:30:43.04 SS.seek 25ml @13:30:57.48 Stroke completed -- waiting for pressure to equalize ... @13:30:57.49 SS.reconfigure :exhale @13:30:57.54 SS.hold @13:31:03.10 Intake.select :close @13:31:03.25 SSV.dial :exhaust @13:31:04.02 Exhaust.select :open @13:31:04.13 SS.seek 0ml @13:31:13.31 Exhaust.select :close @13:31:13.42 SSV.dial CBV @13:31:13.46
CBV.dial SSV @13:31:14.22 Allowing 130s for 25.0ml of remaining 975.0ml @13:31:14.23 Intake.select :open @13:31:16.39 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:31:16.41 SS.reconfigure :inhaling 2.17ml/s @13:31:16.46 SS.seek 25ml @13:31:31.45 Stroke completed -- waiting for pressure to equalize ... @13:31:31.46 SS.reconfigure :exhale @13:31:31.51 SS.hold @13:31:37.31 Intake.select :close @13:31:37.47 SSV.dial :exhaust @13:31:38.24 Exhaust.select :open @13:31:38.35 SS.seek 0ml @13:31:47.69 Exhaust.select :close @13:31:47.80 SSV.dial CBV @13:31:47.82
CBV.dial SSV @13:31:48.61 Allowing 130s for 25.0ml of remaining 950.0ml @13:31:48.62 Intake.select :open @13:31:50.78 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:31:50.80 SS.reconfigure :inhaling 2.17ml/s @13:31:50.85 SS.seek 25ml @13:32:05.22 Stroke completed -- waiting for pressure to equalize ... @13:32:05.23 SS.reconfigure :exhale @13:32:05.27 SS.hold @13:32:11.76 Intake.select :close @13:32:11.92 SSV.dial :exhaust @13:32:12.67 Exhaust.select :open @13:32:12.78 SS.seek 0ml @13:32:21.95 Exhaust.select :close @13:32:22.06 SSV.dial CBV @13:32:22.08
CBV.dial SSV @13:32:22.86 Allowing 130s for 25.0ml of remaining 925.0ml @13:32:22.87 Intake.select :open @13:32:25.04 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:32:25.05 SS.reconfigure :inhaling 2.17ml/s @13:32:25.10 SS.seek 25ml @13:32:40.02 Stroke completed -- waiting for pressure to equalize ... @13:32:40.03 SS.reconfigure :exhale @13:32:40.07 SS.hold @13:32:46.60 Intake.select :close @13:32:46.76 SSV.dial :exhaust @13:32:47.52 Exhaust.select :open @13:32:47.63 SS.seek 0ml @13:32:56.53 Exhaust.select :close @13:32:56.64 SSV.dial CBV @13:32:56.67
CBV.dial SSV @13:32:57.45 Allowing 130s for 25.0ml of remaining 900.0ml @13:32:57.46 Intake.select :open @13:32:59.62 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:32:59.64 SS.reconfigure :inhaling 2.17ml/s @13:32:59.69 SS.seek 25ml @13:33:14.06 Stroke completed -- waiting for pressure to equalize ... @13:33:14.07 SS.reconfigure :exhale @13:33:14.12 SS.hold @13:33:21.58 Intake.select :close @13:33:21.73 SSV.dial :exhaust @13:33:22.50 Exhaust.select :open @13:33:22.62 SS.seek 0ml @13:33:31.73 Exhaust.select :close @13:33:31.84 SSV.dial CBV @13:33:31.87
CBV.dial SSV @13:33:32.64 Allowing 130s for 25.0ml of remaining 875.0ml @13:33:32.65 Intake.select :open @13:33:34.82 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:33:34.83 SS.reconfigure :inhaling 2.17ml/s @13:33:34.88 SS.seek 25ml @13:33:49.45 Stroke completed -- waiting for pressure to equalize ... @13:33:49.46 SS.reconfigure :exhale @13:33:49.51 SS.hold @13:33:58.08 Intake.select :close @13:33:58.23 SSV.dial :exhaust @13:33:59.00 Exhaust.select :open @13:33:59.12 SS.seek 0ml @13:34:07.98 Exhaust.select :close @13:34:08.10 SSV.dial CBV @13:34:08.13
CBV.dial SSV @13:34:08.90 Allowing 130s for 25.0ml of remaining 850.0ml @13:34:08.91 Intake.select :open @13:34:11.43 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:34:11.51 SS.reconfigure :inhaling 2.17ml/s @13:34:11.56 SS.seek 25ml @13:34:25.93 Stroke completed -- waiting for pressure to equalize ... @13:34:25.94 SS.reconfigure :exhale @13:34:25.99 SS.hold @13:34:34.46 Intake.select :close @13:34:34.61 SSV.dial :exhaust @13:34:35.37 Exhaust.select :open @13:34:35.48 SS.seek 0ml @13:34:44.75 Exhaust.select :close @13:34:44.86 SSV.dial CBV @13:34:44.88
CBV.dial SSV @13:34:45.67 Allowing 130s for 25.0ml of remaining 825.0ml @13:34:45.68 Intake.select :open @13:34:47.84 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:34:47.86 SS.reconfigure :inhaling 2.17ml/s @13:34:47.91 SS.seek 25ml @13:35:02.43 Stroke completed -- waiting for pressure to equalize ... @13:35:02.44 SS.reconfigure :exhale @13:35:02.49 SS.hold @13:35:11.47 Intake.select :close @13:35:11.63 SSV.dial :exhaust @13:35:12.39 Exhaust.select :open @13:35:12.50 SS.seek 0ml @13:35:21.62 Exhaust.select :close @13:35:21.73 SSV.dial CBV @13:35:21.77
CBV.dial SSV @13:35:22.51 Allowing 130s for 25.0ml of remaining 800.0ml @13:35:22.52 Intake.select :open @13:35:24.68 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:35:24.70 SS.reconfigure :inhaling 2.17ml/s @13:35:24.75 SS.seek 25ml @13:35:39.42 Stroke completed -- waiting for pressure to equalize ... @13:35:39.43 SS.reconfigure :exhale @13:35:39.47 SS.hold @13:35:49.24 Intake.select :close @13:35:49.39 SSV.dial :exhaust @13:35:50.15 Exhaust.select :open @13:35:50.27 SS.seek 0ml @13:35:59.59 Exhaust.select :close @13:36:00.13 SSV.dial CBV @13:36:00.15
CBV.dial SSV @13:36:00.93 Allowing 130s for 25.0ml of remaining 775.0ml @13:36:00.94 Intake.select :open @13:36:03.11 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:36:03.13 SS.reconfigure :inhaling 2.17ml/s @13:36:03.18 SS.seek 25ml @13:36:17.24 Stroke completed -- waiting for pressure to equalize ... @13:36:17.25 SS.reconfigure :exhale @13:36:17.30 SS.hold @13:36:29.02 Intake.select :close @13:36:29.17 SSV.dial :exhaust @13:36:29.93 Exhaust.select :open @13:36:30.04 SS.seek 0ml @13:36:39.17 Exhaust.select :close @13:36:39.28 SSV.dial CBV @13:36:39.30
CBV.dial SSV @13:36:40.09 Allowing 130s for 25.0ml of remaining 750.0ml @13:36:40.10 Intake.select :open @13:36:42.27 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:36:42.29 SS.reconfigure :inhaling 2.17ml/s @13:36:42.34 SS.seek 25ml @13:36:57.02 Stroke completed -- waiting for pressure to equalize ... @13:36:57.03 SS.reconfigure :exhale @13:36:57.08 SS.hold @13:37:09.84 Intake.select :close @13:37:09.99 SSV.dial :exhaust @13:37:10.76 Exhaust.select :open @13:37:10.87 SS.seek 0ml @13:37:20.27 Exhaust.select :close @13:37:20.38 SSV.dial CBV @13:37:20.41
CBV.dial SSV @13:37:21.18 Allowing 130s for 25.0ml of remaining 725.0ml @13:37:21.19 Intake.select :open @13:37:23.36 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:37:23.38 SS.reconfigure :inhaling 2.17ml/s @13:37:23.42 SS.seek 25ml @13:37:37.99 Stroke completed -- waiting for pressure to equalize ... @13:37:38.00 SS.reconfigure :exhale @13:37:38.05 SS.hold @13:37:52.84 Intake.select :close @13:37:52.99 SSV.dial :exhaust @13:37:53.76 Exhaust.select :open @13:37:53.87 SS.seek 0ml @13:38:03.20 Exhaust.select :close @13:38:03.31 SSV.dial CBV @13:38:03.33
CBV.dial SSV @13:38:04.12 Allowing 130s for 25.0ml of remaining 700.0ml @13:38:04.13 Intake.select :open @13:38:06.29 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:38:06.31 SS.reconfigure :inhaling 2.17ml/s @13:38:06.36 SS.seek 25ml @13:38:20.35 Stroke completed -- waiting for pressure to equalize ... @13:38:20.36 SS.reconfigure :exhale @13:38:20.41 SS.hold @13:38:38.76 Intake.select :close @13:38:38.91 SSV.dial :exhaust @13:38:39.68 Exhaust.select :open @13:38:39.79 SS.seek 0ml @13:38:48.66 Exhaust.select :close @13:38:48.77 SSV.dial CBV @13:38:48.79
CBV.dial SSV @13:38:49.57 Allowing 130s for 25.0ml of remaining 675.0ml @13:38:49.58 Intake.select :open @13:38:51.75 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:38:51.76 SS.reconfigure :inhaling 2.17ml/s @13:38:51.81 SS.seek 25ml @13:39:04.89 Vacuum too high -- waiting up to 40 seconds @13:39:04.90 SS.reconfigure :exhale @13:39:04.96 SS.hold @13:39:18.00 Intake @12.6psia, vacuum from 7.1 to 10.1psi @13:39:18.02 SS.reconfigure :inhaling 1.95ml/s @13:39:18.07 SS.seek 25ml @13:39:20.28 Stroke completed -- waiting for pressure to equalize ... @13:39:20.29 SS.reconfigure :exhale @13:39:20.33 SS.hold @13:39:27.10 Intake.select :close @13:39:27.25 SSV.dial :exhaust @13:39:28.02 Exhaust.select :open @13:39:28.13 SS.seek 0ml @13:39:37.51 Exhaust.select :close @13:39:37.62 SSV.dial CBV @13:39:37.64
CBV.dial SSV @13:39:38.43 Allowing 130s for 25.0ml of remaining 650.0ml @13:39:38.44 Intake.select :open @13:39:40.61 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:39:40.63 SS.reconfigure :inhaling 1.95ml/s @13:39:40.68 SS.seek 25ml @13:39:49.83 Vacuum too high -- waiting up to 40 seconds @13:39:49.84 SS.reconfigure :exhale @13:39:49.89 SS.hold @13:39:59.22 Intake @13.1psia, vacuum from 7.6 to 10.6psi @13:39:59.24 SS.reconfigure :inhaling 1.77ml/s @13:39:59.29 SS.seek 25ml @13:40:04.51 Vacuum too high -- waiting up to 40 seconds @13:40:04.53 SS.reconfigure :exhale @13:40:04.58 SS.hold @13:40:12.36 SS.reconfigure :inhaling 1.59ml/s @13:40:12.41 SS.seek 25ml @13:40:15.16 Stroke completed -- waiting for pressure to equalize ... @13:40:15.17 SS.reconfigure :exhale @13:40:15.22 SS.hold @13:40:20.84 Intake.select :close @13:40:21.00 SSV.dial :exhaust @13:40:22.29 Exhaust.select :open @13:40:22.40 SS.seek 0ml @13:40:31.60 Exhaust.select :close @13:40:31.71 SSV.dial CBV @13:40:31.74
CBV.dial SSV @13:40:32.52 Allowing 130s for 25.0ml of remaining 625.0ml @13:40:32.53 Intake.select :open @13:40:34.70 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:40:34.72 SS.reconfigure :inhaling 1.59ml/s @13:40:34.77 SS.seek 25ml @13:40:45.48 Vacuum too high -- waiting up to 40 seconds @13:40:45.49 SS.reconfigure :exhale @13:40:45.55 SS.hold @13:40:54.33 Intake @13.4psia, vacuum from 7.9 to 10.9psi @13:40:54.35 SS.reconfigure :inhaling 1.43ml/s @13:40:54.39 SS.seek 25ml @13:41:00.54 Vacuum too high -- waiting up to 40 seconds @13:41:00.56 SS.reconfigure :exhale @13:41:00.61 SS.hold @13:41:08.29 Intake @13.1psia, vacuum from 7.6 to 10.6psi @13:41:08.31 SS.reconfigure :inhaling 1.29ml/s @13:41:08.36 SS.seek 25ml @13:41:11.24 Stroke completed -- waiting for pressure to equalize ... @13:41:11.25 SS.reconfigure :exhale @13:41:11.30 SS.hold @13:41:17.81 Intake.select :close @13:41:17.96 SSV.dial :exhaust @13:41:18.72 Exhaust.select :open @13:41:18.83 SS.seek 0ml @13:41:27.80 Exhaust.select :close @13:41:27.91 SSV.dial CBV @13:41:27.94
CBV.dial SSV @13:41:28.72 Allowing 130s for 25.0ml of remaining 600.0ml @13:41:28.73 Intake.select :open @13:41:30.89 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:41:30.91 SS.reconfigure :inhaling 1.29ml/s @13:41:30.96 SS.seek 25ml @13:41:43.54 Vacuum too high -- waiting up to 40 seconds @13:41:43.55 SS.reconfigure :exhale @13:41:43.61 SS.hold @13:41:52.46 Intake @13.4psia, vacuum from 7.9 to 10.9psi @13:41:52.48 SS.reconfigure :inhaling 1.16ml/s @13:41:52.53 SS.seek 25ml @13:42:00.48 Vacuum too high -- waiting up to 40 seconds @13:42:00.49 SS.reconfigure :exhale @13:42:00.55 SS.hold @13:42:10.43 Intake @13.7psia, vacuum from 8.2 to 11.2psi @13:42:10.45 SS.reconfigure :inhaling 1.04ml/s @13:42:10.50 SS.seek 25ml @13:42:14.44 Stroke completed -- waiting for pressure to equalize ... @13:42:14.45 SS.reconfigure :exhale @13:42:14.50 SS.hold @13:42:20.44 Intake.select :close @13:42:20.59 SSV.dial :exhaust @13:42:21.35 Exhaust.select :open @13:42:21.46 SS.seek 0ml @13:42:30.39 Exhaust.select :close @13:42:30.50 SSV.dial CBV @13:42:30.52
CBV.dial SSV @13:42:31.35 Allowing 130s for 25.0ml of remaining 575.0ml @13:42:31.36 Intake.select :open @13:42:33.53 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:42:33.55 SS.reconfigure :inhaling 1.04ml/s @13:42:33.59 SS.seek 25ml @13:42:48.04 Vacuum too high -- waiting up to 40 seconds @13:42:48.05 SS.reconfigure :exhale @13:42:48.11 SS.hold @13:42:56.15 Intake @13.3psia, vacuum from 7.8 to 10.8psi @13:42:56.17 SS.reconfigure :inhaling 0.94ml/s @13:42:56.22 SS.seek 25ml @13:43:05.38 Vacuum too high -- waiting up to 40 seconds @13:43:05.40 SS.reconfigure :exhale @13:43:05.45 SS.hold @13:43:14.12 Intake @13.4psia, vacuum from 7.9 to 10.9psi @13:43:14.14 SS.reconfigure :inhaling 0.85ml/s @13:43:14.19 SS.seek 25ml @13:43:19.48 Stroke completed -- waiting for pressure to equalize ... @13:43:19.49 SS.reconfigure :exhale @13:43:19.54 SS.hold @13:43:28.41 Intake.select :close @13:43:28.56 SSV.dial :exhaust @13:43:29.33 Exhaust.select :open @13:43:29.45 SS.seek 0ml @13:43:38.80 Exhaust.select :close @13:43:38.91 SSV.dial CBV @13:43:38.93
CBV.dial SSV @13:43:39.72 Allowing 130s for 25.0ml of remaining 550.0ml @13:43:39.73 Intake.select :open @13:43:41.90 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:43:41.92 SS.reconfigure :inhaling 0.85ml/s @13:43:41.97 SS.seek 25ml @13:44:01.91 Vacuum too high -- waiting up to 40 seconds @13:44:01.93 SS.reconfigure :exhale @13:44:01.98 SS.hold @13:44:10.68 Intake @13.5psia, vacuum from 8.0 to 11.0psi @13:44:10.69 SS.reconfigure :inhaling 0.77ml/s @13:44:10.74 SS.seek 25ml @13:44:23.56 Stroke completed -- waiting for pressure to equalize ... @13:44:23.57 SS.reconfigure :exhale @13:44:23.62 SS.hold @13:44:40.43 Intake.select :close @13:44:40.59 SSV.dial :exhaust @13:44:41.34 Exhaust.select :open @13:44:41.45 SS.seek 0ml @13:44:50.81 Exhaust.select :close @13:44:50.92 SSV.dial CBV @13:44:50.94
CBV.dial SSV @13:44:51.75 Allowing 130s for 25.0ml of remaining 525.0ml @13:44:51.76 Intake.select :open @13:44:53.92 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:44:53.94 SS.reconfigure :inhaling 0.77ml/s @13:44:53.99 SS.seek 25ml @13:45:13.97 Vacuum too high -- waiting up to 40 seconds @13:45:13.99 SS.reconfigure :exhale @13:45:14.04 SS.hold @13:45:22.75 Intake @13.4psia, vacuum from 7.9 to 10.9psi @13:45:22.76 SS.reconfigure :inhaling 0.70ml/s @13:45:22.81 SS.seek 25ml @13:45:39.07 Vacuum too high -- waiting up to 40 seconds @13:45:39.08 SS.reconfigure :exhale @13:45:39.14 SS.hold @13:45:51.90 Intake @13.7psia, vacuum from 8.2 to 11.2psi @13:45:51.92 SS.reconfigure :inhaling 0.62ml/s @13:45:51.97 SS.seek 25ml @13:45:52.78 Stroke completed -- waiting for pressure to equalize ... @13:45:52.79 SS.reconfigure :exhale @13:45:52.83 SS.hold @13:45:58.01 Intake.select :close @13:45:58.16 SSV.dial :exhaust @13:45:58.92 Exhaust.select :open @13:45:59.03 SS.seek 0ml @13:46:08.36 Exhaust.select :close @13:46:08.47 SSV.dial CBV @13:46:08.50
CBV.dial SSV @13:46:09.26 Allowing 130s for 25.0ml of remaining 500.0ml @13:46:09.27 Intake.select :open @13:46:11.44 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:46:11.46 SS.reconfigure :inhaling 0.62ml/s @13:46:11.51 SS.seek 25ml @13:46:32.16 Vacuum too high -- waiting up to 40 seconds @13:46:32.17 SS.reconfigure :exhale @13:46:32.23 SS.hold @13:46:40.33 Intake @14.1psia, vacuum from 8.6 to 11.6psi @13:46:40.35 SS.reconfigure :inhaling 0.56ml/s @13:46:40.40 SS.seek 25ml @13:46:54.47 Vacuum too high -- waiting up to 40 seconds @13:46:54.49 SS.reconfigure :exhale @13:46:54.54 SS.hold @13:47:03.22 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:47:03.24 SS.reconfigure :inhaling 0.51ml/s @13:47:03.28 SS.seek 25ml @13:47:14.15 Stroke completed -- waiting for pressure to equalize ... @13:47:14.16 SS.reconfigure :exhale @13:47:14.21 SS.hold @13:47:26.87 Intake.select :close @13:47:27.02 SSV.dial :exhaust @13:47:27.79 Exhaust.select :open @13:47:27.91 SS.seek 0ml @13:47:37.23 Exhaust.select :close @13:47:37.34 SSV.dial CBV @13:47:37.38
CBV.dial SSV @13:47:38.14 Allowing 130s for 25.0ml of remaining 475.0ml @13:47:38.15 Intake.select :open @13:47:40.31 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:47:40.33 SS.reconfigure :inhaling 0.51ml/s @13:47:40.38 SS.seek 25ml @13:48:11.72 Vacuum too high -- waiting up to 40 seconds @13:48:11.73 SS.reconfigure :exhale @13:48:11.79 SS.hold @13:48:17.52 Intake @13.6psia, vacuum from 8.1 to 11.1psi @13:48:17.54 SS.reconfigure :inhaling 0.46ml/s @13:48:17.60 SS.seek 25ml @13:48:39.41 Vacuum too high -- waiting up to 40 seconds @13:48:39.42 SS.reconfigure :exhale @13:48:39.47 SS.hold @13:48:50.99 Intake @14.1psia, vacuum from 8.6 to 11.6psi @13:48:51.01 SS.reconfigure :inhaling 0.42ml/s @13:48:51.06 SS.seek 25ml @13:48:51.31 Stroke completed -- waiting for pressure to equalize ... @13:48:51.32 SS.reconfigure :exhale @13:48:51.36 SS.hold @13:48:58.83 Intake.select :close @13:48:58.99 SSV.dial :exhaust @13:49:00.11 Exhaust.select :open @13:49:00.30 SS.seek 0ml @13:49:09.57 Exhaust.select :close @13:49:09.68 SSV.dial CBV @13:49:09.71
CBV.dial SSV @13:49:10.48 Allowing 130s for 25.0ml of remaining 450.0ml @13:49:10.49 Intake.select :open @13:49:12.66 Intake @14.8psia, vacuum from 9.3 to 12.3psi @13:49:12.68 SS.reconfigure :inhaling 0.42ml/s @13:49:12.73 SS.seek 25ml @13:49:53.19 Vacuum too high -- waiting up to 40 seconds @13:49:53.20 SS.reconfigure :exhale @13:49:53.26 SS.hold @13:50:01.79 Intake @13.8psia, vacuum from 8.3 to 11.3psi @13:50:01.81 SS.reconfigure :inhaling 0.38ml/s @13:50:01.86 SS.seek 25ml @13:50:26.12 Stroke completed -- waiting for pressure to equalize ... @13:50:26.13 SS.reconfigure :exhale @13:50:26.17 SS.hold @13:50:46.56 Intake.select :close @13:50:46.76 SSV.dial :exhaust @13:50:47.53 Exhaust.select :open @13:50:47.65 SS.seek 0ml @13:50:56.99 Exhaust.select :close @13:50:57.10 SSV.dial CBV @13:50:57.12
CBV.dial SSV @13:50:57.92 Allowing 130s for 25.0ml of remaining 425.0ml @13:50:57.93 Intake.select :open @13:51:00.09 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:51:00.11 SS.reconfigure :inhaling 0.38ml/s @13:51:00.16 SS.seek 25ml @13:51:50.44 Vacuum too high -- waiting up to 40 seconds @13:51:50.45 SS.reconfigure :exhale @13:51:50.50 SS.hold @13:52:00.44 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:52:00.46 SS.reconfigure :inhaling 0.33ml/s @13:52:00.51 SS.seek 25ml @13:52:21.91 Stroke completed -- waiting for pressure to equalize ... @13:52:21.92 SS.reconfigure :exhale @13:52:21.97 SS.hold @13:52:40.36 Intake.select :close @13:52:40.51 SSV.dial :exhaust @13:52:41.27 Exhaust.select :open @13:52:41.38 SS.seek 0ml @13:52:50.38 Exhaust.select :close @13:52:50.92 SSV.dial CBV @13:52:50.96
CBV.dial SSV @13:52:51.71 Allowing 130s for 25.0ml of remaining 400.0ml @13:52:51.72 Intake.select :open @13:52:53.94 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:52:53.96 SS.reconfigure :inhaling 0.33ml/s @13:52:54.01 SS.seek 25ml @13:53:43.78 Vacuum too high -- waiting up to 40 seconds @13:53:43.79 SS.reconfigure :exhale @13:53:43.85 SS.hold @13:53:53.64 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:53:53.66 SS.reconfigure :inhaling 0.30ml/s @13:53:53.71 SS.seek 25ml @13:54:21.09 Vacuum too high -- waiting up to 40 seconds @13:54:21.10 SS.reconfigure :exhale @13:54:21.15 SS.hold @13:54:42.11 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:54:42.13 SS.reconfigure :inhaling 0.28ml/s @13:54:42.18 SS.seek 25ml @13:54:48.91 Stroke completed -- waiting for pressure to equalize ... @13:54:48.92 SS.reconfigure :exhale @13:54:48.97 SS.hold @13:55:01.73 SS.reconfigure :default @13:55:01.78 SS.hold @13:55:01.85 Intake.select :close @13:55:02.01 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 625.0ml @13:55:02.02 SSV.dial :exhaust @13:55:02.79 Exhaust.select :open @13:55:02.90 SS.reconfigure :exhale @13:55:02.95 SS.seek 0ml @13:55:12.32 Exhaust.select :close @13:55:12.43 Sampled 625.0ml @13:55:12.44 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:55:12.45 Intake.select :close @13:55:12.52 CSV.dial :waste @13:55:12.68 SSV.dial CBV,avoiding: :exhaust @13:55:12.71 CS.seek 0ml @13:55:12.74
CBV.dial SSV @13:55:12.88 CSV.dial :air @13:55:13.60 CS.seek 10ml @13:55:14.65
CTV.dial :air @13:55:15.44 delay 15 seconds @13:55:30.46 SSV.dial :spit,avoiding: :exhaust @13:55:31.79 CTV.dial CSV,avoiding: :intake @13:55:31.83
SS.seek 0ml @13:55:31.95 SSV.dial CBV,avoiding: :exhaust @13:55:37.63 SSV.dial between CBV and plug @13:55:38.13 CBV.dial between SSV and waste2 @13:55:38.54 CTV.dial CSV,avoiding: :air @13:55:38.56
CSV.dial CTV @13:55:39.04 CS.reconfigure :pressurize @13:55:39.09 CS.seek 0ml @13:56:02.64 BubblePoint: Intake @24.3psia, Outlet @0.1psia @13:56:02.65 CBV.dial SSV @13:56:02.99 SS.reconfigure :default @13:56:03.04 SS.seek 10ml @13:56:08.67 delay 15 seconds @13:56:23.80 Evacuated: Intake @23.9psia, Outlet @23.5psia @13:56:23.82 SSV.dial :spit,avoiding: :exhaust @13:56:24.88 SS.reconfigure :exhale @13:56:24.93 SS.seek 0ml @13:56:29.90 SS.reconfigure :fast @13:56:29.95 SS.seek 5ml @13:56:33.07 SSV.dial CBV,avoiding: :exhaust @13:56:33.10
CBV.dial SSV @13:56:34.46 CTV.dial :air,avoiding: :intake @13:56:35.83 CSV.dial CTV @13:56:35.87
SS.seek 10ml @13:56:37.42 CSV.dial :air @13:56:37.89 CS.reconfigure :fast @13:56:37.94 CS.seek 0ml @13:56:39.32
SSV.dial :spit,avoiding: :exhaust @13:56:40.65 SS.reconfigure :exhale @13:56:40.70 SS.seek 0ml @13:56:41.06 CS.stop @13:56:45.63
SS.reconfigure :fast @13:56:45.68 SS.seek 5ml @13:56:48.49 SSV.dial CBV,avoiding: :exhaust @13:56:48.52
CBV.dial SSV @13:56:50.87 SSV.dial :spit,avoiding: :exhaust @13:56:52.19 SS.reconfigure :default @13:56:52.24 SS.seek 0ml @13:56:55.48 SS.stop @13:56:55.52 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:56:55.53 Intake.select :close @13:56:55.60 CSV.dial :waste @13:56:55.73 SSV.dial CBV,avoiding: :exhaust @13:56:55.75
CBV.dial SSV @13:56:56.34 CS.seek 0ml @13:56:56.41 CSV.dial :air @13:56:57.12
CTV.dial :air @13:56:57.21 delay 15 seconds @13:56:57.23 CS.seek 10ml @13:57:12.23
SSV.dial :spit,avoiding: :exhaust @13:57:13.57 CTV.dial CSV,avoiding: :intake @13:57:13.60
SS.reconfigure :exhale @13:57:13.71 SS.seek 0ml @13:57:13.77 SSV.dial CBV,avoiding: :exhaust @13:57:21.04 SSV.dial between CBV and plug @13:57:21.52 CBV.dial between SSV and waste2 @13:57:21.92 CSV.dial between air and CTV @13:57:22.23 CS.seek 8.068ml @13:57:27.44 CTV.dial CSV,avoiding: :air @13:57:27.47
CSV.dial CTV @13:57:27.80 CS.reconfigure :pressurize @13:57:27.85 CS.seek 0ml @13:57:46.51 BubblePoint: Intake @24.2psia, Outlet @-0.2psia @13:57:46.52 CBV.dial SSV @13:57:46.87 SS.reconfigure :default @13:57:46.91 SS.seek 10ml @13:57:52.61 delay 15 seconds @13:58:07.74 Evacuated: Intake @23.0psia, Outlet @16.6psia @13:58:07.75 SSV.dial :spit,avoiding: :exhaust @13:58:09.26 SS.reconfigure :exhale @13:58:09.31 SS.seek 0ml @13:58:14.39 SS.reconfigure :fast @13:58:14.43 SS.seek 5ml @13:58:17.24 SSV.dial CBV,avoiding: :exhaust @13:58:17.26
CBV.dial SSV @13:58:18.64 CTV.dial :air,avoiding: :intake @13:58:20.04 CSV.dial CTV @13:58:20.07
SS.seek 10ml @13:58:21.29 CSV.dial :air @13:58:21.73 CS.reconfigure :fast @13:58:21.78 CS.seek 0ml @13:58:22.53
SSV.dial :spit,avoiding: :exhaust @13:58:23.87 SS.reconfigure :exhale @13:58:23.91 SS.seek 0ml @13:58:25.04 CS.stop @13:58:29.04
SS.reconfigure :fast @13:58:29.09 SS.seek 5ml @13:58:31.91 SSV.dial CBV,avoiding: :exhaust @13:58:31.94
CBV.dial SSV @13:58:34.30 SSV.dial :spit,avoiding: :exhaust @13:58:35.63 SS.reconfigure :default @13:58:35.68 SS.seek 0ml @13:58:38.70 SS.stop @13:58:38.75 Lysing: Adding 2.0ml AqMeOH @13:58:38.76 CBV.dial between CSV and waste1,avoiding: :DBV @13:58:40.25 CTV.dial CSV @13:58:41.61 CSV.dial CSR @13:58:42.40 Pulling 1ml of CSR.AqMeOH @13:58:42.41 CSR.select :AqMeOH @13:58:42.53 CS.reconfigure :default @13:58:42.58 CS.seek 1ml @13:58:46.06 delay 5 seconds @13:58:51.08 CSR.select :air @13:58:51.25 CS.seek 6ml @13:59:04.81 delay 2 seconds @13:59:06.83 CSV.dial :waste2 @13:59:07.27 CS.reconfigure :fast @13:59:07.32 CS.seek 0ml @13:59:21.75 CSV.dial CSR @13:59:22.20 Pulling 2ml of CSR.AqMeOH @13:59:22.21 CSR.select :AqMeOH @13:59:22.33 CS.reconfigure :default @13:59:22.38 CS.seek 2ml @13:59:28.34 delay 5 seconds @13:59:33.36 CSR.select :air @13:59:33.53 CS.seek 5ml @13:59:42.00 delay 2 seconds @13:59:44.02 CSV.dial CTV @13:59:44.52 CS.seek 1ml @13:59:55.74 Before lysing Collection Puck Heater at 18.8C/15.5psia @13:59:55.76 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @13:59:55.78 CH.seek 70C,8:00 @14:02:13.79 CH.hold 10:00,70C @14:02:13.99 Begun lysing Collection Puck Heater at 68.1C/21.8psia @14:12:14.01 Ended lysing Collection Puck Heater at 70C/20.9psia @14:12:14.02 CS.seek 0ml @14:12:17.46 CTV.dial between spare and air,avoiding: :intake @14:12:18.52 CSV.dial :air @14:12:18.97 CS.seek 5ml @14:12:32.61 CSV.dial CTV @14:12:33.07 delay 2 seconds @14:12:35.08 CSV.dial :air @14:12:35.54 delay 2 seconds @14:12:37.55 CSV.dial CTV @14:12:38.01 delay 2 seconds @14:12:40.02 CSV.dial :air @14:12:40.47 delay 2 seconds @14:12:42.50 CS.seek 0ml @14:12:56.11 Puck @28.6psia after final lysis pressurization @14:12:56.12 Recovering Lysate into Collection syringe @14:12:56.13 CSV.dial CBV @14:12:57.11 CBV.dial CSV @14:12:57.50 CS.seek 5ml @14:13:11.27 delay 5 seconds @14:13:16.29 CTV.dial :air @14:13:16.82 CS.seek 7ml @14:13:22.74 delay 5 seconds @14:13:27.76 CSV.dial :air2 @14:13:28.20 CS.seek 10ml @14:13:36.68 CSV.dial between air and CTV,avoiding: CBV @14:13:37.93 Lysing Complete @14:13:38.00 Storing sh1 puck in Collection Clamp to tube 1 @14:13:38.17 SC.reconfigure :tight @14:13:38.60 SC.seek 1 @14:13:42.19 SC.configure :limp @14:13:42.42 Elbow.reconfigure :tight @14:13:42.47 Elbow.seek 1 @14:13:44.16 Forearm.reconfigure :tight @14:13:44.21 Forearm.seek 1 + 800 counts @14:13:45.28 SE.reconfigure :pick @14:13:45.34 SE.seek :beamBreak @14:13:47.66 SE.configure :default @14:13:47.82 Elbow.reconfigure :limp @14:13:47.87 Forearm.reconfigure :loose @14:13:47.92 Forearm.seek 1 @14:13:48.32 Elbow.hold @14:13:48.39 Elbow.reconfigure :tight @14:13:48.49 SE.seek 7.11 Pucks below Top Plate @14:13:48.98 Forearm.force -20 @14:13:49.02 Forearm.seek 1 + 316 counts @14:13:49.30 Forearm.reconfigure :tight @14:13:49.35 Forearm.seek 1 + 800 counts @14:13:49.64 SE.seek 8.11 Pucks below Top Plate @14:13:50.22 Forearm.reconfigure :loose @14:13:50.27 Forearm.seek :retract @14:13:51.25 Elbow.seek CC @14:13:52.86 Elbow.reconfigure :loose @14:13:52.96 Forearm.seek CC @14:13:53.93 Hand.close @14:13:54.64 CC.seek :open @14:13:58.84 Forearm.seek :retract @14:13:59.87 Elbow.reconfigure :tight @14:14:00.30 Elbow.seek 1 @14:14:02.09 Elbow.reconfigure :limp @14:14:02.14 Forearm.seek 1 @14:14:03.28 Elbow.hold @14:14:03.35 Elbow.reconfigure :tight @14:14:03.40 Hand.open @14:14:04.26 Forearm.force -20 @14:14:04.30 Forearm.seek 1 + 316 counts @14:14:04.72 Forearm.reconfigure :tight @14:14:04.77 Forearm.seek 1 + 800 counts @14:14:05.13 SE.seek :down @14:14:06.26
Forearm.reconfigure :loose @14:14:06.31 Forearm.seek :retract @14:14:07.07 SE.stop @14:14:07.26
Tranferring DA @14:14:07.28 Beginning samplePassC @14:14:07.29 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:14:07.48 Elbow.seek FlushPuck::Garage @14:14:09.26 Elbow.reconfigure :loose @14:14:09.37 Forearm.seek :garage @14:14:10.43 Hand.close @14:14:11.26 Forearm.seek :retract @14:14:12.26 Elbow.reconfigure :tight @14:14:12.31 Elbow.seek CC @14:14:15.13 Elbow.reconfigure :loose @14:14:15.23 Forearm.reconfigure :tight @14:14:15.28 Forearm.seek CC @14:14:17.14 CC.seek :closed @14:14:21.12 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.00 @14:14:21.19 Hand.open @14:14:21.95 Forearm.reconfigure :loose @14:14:22.00 Forearm.seek :retract @14:14:23.09 PSV.dial PSR @14:14:24.45 Pulling 1.5ml of PSR.flush @14:14:24.46 PSR.select :flush @14:14:24.59 PS.seek 1.5ml @14:14:40.76 delay 5 seconds @14:14:45.78 PSV.dial :waste1 @14:14:46.36 PS.seek 0ml @14:15:02.54 PSV.dial PSR @14:15:02.97 PSR.select :air @14:15:03.10 PS.seek 2.5ml @14:15:29.48 PRV.dial CBV,avoiding: :ab2 @14:15:29.51
CBV.dial PRV @14:15:31.24 PSV.dial :mixloop @14:15:32.26 CTV.dial :air,avoiding: :intake @14:15:32.33 PTV.dial :PRVmixing @14:15:33.62 PS.seek 0ml @14:15:59.97 PSV.dial :air @14:16:00.43 PS.seek 2.5ml @14:16:26.82 PSV.dial :mixloop @14:16:27.28 PS.seek 0ml @14:16:53.66 PSV.dial :air @14:16:54.12 PS.seek 2.5ml @14:17:20.47 PSV.dial :mixloop @14:17:20.93 PS.seek 0ml @14:17:47.32 Garaging FlushPuck in Collection Clamp @14:17:47.39 Elbow.reconfigure :tight @14:17:47.44 Elbow.seek CC @14:17:47.55 Elbow.reconfigure :loose @14:17:48.11 Forearm.seek CC @14:17:49.12 Hand.close @14:17:49.89 CC.seek :open @14:17:53.98 Forearm.seek :retract @14:17:54.98 Elbow.reconfigure :tight @14:17:55.03 Elbow.seek :garage @14:17:57.89 Elbow.reconfigure :loose @14:17:58.00 Forearm.reconfigure :tight @14:17:58.04 Forearm.seek :garage @14:17:59.17 Hand.open @14:17:59.95 Forearm.reconfigure :loose @14:17:59.99 Forearm.seek :retract @14:18:00.98 PSV.dial :mixloop @14:18:01.05 PTV.dial :PRVmixing @14:18:01.13 PRV.dial CBV @14:18:01.16
CBV.dial PRV @14:18:01.39 CTV.dial :air,avoiding: :intake @14:18:01.41 Loading lyfil puck from tube 2 into Collection Clamp @14:18:01.65 SC.reconfigure :tight @14:18:01.70 SC.seek 2 @14:18:05.34 SC.configure :limp @14:18:05.56 Elbow.reconfigure :tight @14:18:05.61 Elbow.seek 2 @14:18:07.49 Forearm.reconfigure :tight @14:18:07.54 Forearm.seek 2 + 800 counts @14:18:08.57 SE.reconfigure :pick @14:18:08.63 SE.seek :beamBreak @14:18:10.54 SE.configure :default @14:18:10.71 Elbow.seek 2 @14:18:11.33 Forearm.seek 2 + 300 counts @14:18:11.51 Forearm.reconfigure :loose @14:18:11.56 Hand.close @14:18:12.27 SE.seek :down @14:18:12.48
Forearm.seek :retract @14:18:13.45 Elbow.seek CC @14:18:13.91 SE.stop @14:18:15.02
Elbow.reconfigure :loose @14:18:15.12 Forearm.reconfigure :tight @14:18:15.17 Forearm.seek CC @14:18:16.46 CC.seek :closed @14:18:20.43 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.03 @14:18:20.44 Hand.open @14:18:21.20 Forearm.reconfigure :loose @14:18:21.25 Forearm.seek :retract @14:18:22.21 CTV.dial CSV,avoiding: :intake @14:18:23.59 CSV.dial CTV @14:18:23.90 PS.seek 2.5ml @14:18:50.28 CS.seek 5ml @14:19:03.95 delay 8 seconds @14:19:11.97 PSV.dial :air @14:19:12.43 delay 2 seconds @14:19:14.44 PSV.dial :waste2 @14:19:15.18 PS.seek 0ml @14:19:41.53 CBV.dial between PRV and waste1 @14:19:41.86 CSV.dial :air @14:19:42.32 CS.seek 10ml @14:19:55.92 CSV.dial CTV @14:19:56.82 CS.seek 0ml @14:20:23.23 CBV.dial :waste1 @14:20:23.55 delay 5 seconds @14:20:28.57 CTV.dial :air,avoiding: :intake @14:20:30.00 Storing lyfil puck in Collection Clamp to tube 1 @14:20:30.17 SC.reconfigure :tight @14:20:30.22 SC.seek 1 @14:20:33.83 SC.configure :limp @14:20:34.05 Elbow.reconfigure :tight @14:20:34.10 Elbow.seek 1 @14:20:35.85 Forearm.reconfigure :tight @14:20:35.90 Forearm.seek 1 + 800 counts @14:20:36.99 SE.reconfigure :pick @14:20:37.05 SE.seek :beamBreak @14:20:39.35 SE.configure :default @14:20:39.51 Elbow.reconfigure :limp @14:20:39.56 Forearm.reconfigure :loose @14:20:39.61 Forearm.seek 1 @14:20:39.97 Elbow.hold @14:20:40.04 Elbow.reconfigure :tight @14:20:40.14 SE.seek 8.1 Pucks below Top Plate @14:20:40.53 Forearm.force -20 @14:20:40.58 Forearm.seek 1 + 292 counts @14:20:40.97 Forearm.reconfigure :tight @14:20:41.02 Forearm.seek 1 + 800 counts @14:20:41.32 SE.seek 9.1 Pucks below Top Plate @14:20:41.99 Forearm.reconfigure :loose @14:20:42.04 Forearm.seek :retract @14:20:43.13 Elbow.seek CC @14:20:45.20 Elbow.reconfigure :loose @14:20:45.31 Forearm.seek CC @14:20:46.22 Hand.close @14:20:47.01 CC.seek :open @14:20:51.22 Forearm.seek :retract @14:20:52.30 Elbow.reconfigure :tight @14:20:52.35 Elbow.seek 1 @14:20:54.04 Elbow.reconfigure :limp @14:20:54.10 Forearm.seek 1 @14:20:55.19 Elbow.hold @14:20:55.26 Elbow.reconfigure :tight @14:20:55.31 Hand.open @14:20:56.15 Forearm.force -20 @14:20:56.20 Forearm.seek 1 + 319 counts @14:20:56.56 Forearm.reconfigure :tight @14:20:56.61 Forearm.seek 1 + 800 counts @14:20:57.02 SE.seek :down @14:20:58.17
Forearm.reconfigure :loose @14:20:58.22 Forearm.seek :retract @14:20:58.87 SE.stop @14:20:59.11
PSV.dial :air @14:20:59.85 PS.seek 2.5ml @14:21:26.28 delay 1 second @14:21:27.30 PSV.dial :mixloop @14:21:27.74 delay 1 second @14:21:28.75 PSV.dial :air @14:21:29.20 delay 1 second @14:21:30.22 PSV.dial :mixloop @14:21:30.67 delay 1 second @14:21:31.69 PSV.dial :air @14:21:32.14 delay 1 second @14:21:33.16 PS.seek 0ml @14:22:00.10 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:22:00.29 Elbow.seek FlushPuck::Garage @14:22:02.08 Elbow.reconfigure :loose @14:22:02.18 Forearm.seek :garage @14:22:03.22 Hand.close @14:22:04.13 Forearm.seek :retract @14:22:05.14 Elbow.reconfigure :tight @14:22:05.19 Elbow.seek PC @14:22:06.50 Elbow.reconfigure :loose @14:22:06.60 Forearm.reconfigure :tight @14:22:06.65 Forearm.seek PC @14:22:08.02 PC.seek :closed @14:22:12.45 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.58 @14:22:12.51 Hand.open @14:22:13.31 Forearm.reconfigure :loose @14:22:13.36 Forearm.seek :retract @14:22:14.44 PRV.dial :air @14:22:14.91 PSV.dial :mixloop @14:22:15.43 PS.seek 0.3ml @14:22:19.31 PSV.dial PSR @14:22:20.34 Pulling 0.4ml of PSR.air @14:22:20.36 PSR.select :air @14:22:20.38 PS.seek 0.7ml @14:22:25.25 delay 3 seconds @14:22:28.27 PSR.select :air @14:22:28.29 PS.seek 2.5ml @14:22:47.47 delay 3 seconds @14:22:50.48 PSV.dial :mixloop @14:22:51.53 PTV.dial :mixing @14:22:52.26 PBV.dial :waste2 @14:22:53.24 PS.seek 0ml @14:23:19.66 Garaging FlushPuck in Processing Clamp @14:23:19.73 Elbow.reconfigure :tight @14:23:19.78 Elbow.seek PC @14:23:19.95 Elbow.reconfigure :loose @14:23:20.06 Forearm.seek PC @14:23:21.16 Hand.close @14:23:21.88 PC.seek :open @14:23:26.37 Forearm.seek :retract @14:23:27.44 Elbow.reconfigure :tight @14:23:27.49 Elbow.seek :garage @14:23:28.92 Elbow.reconfigure :loose @14:23:29.02 Forearm.reconfigure :tight @14:23:29.07 Forearm.seek :garage @14:23:30.20 Hand.open @14:23:30.94 Forearm.reconfigure :loose @14:23:30.99 Forearm.seek :retract @14:23:32.11 Loading da puck from tube 2 into Processing Clamp @14:23:32.36 SC.reconfigure :tight @14:23:32.40 SC.seek 2 @14:23:36.26 SC.configure :limp @14:23:36.47 Elbow.reconfigure :tight @14:23:36.52 Elbow.seek 2 @14:23:38.39 Forearm.reconfigure :tight @14:23:38.43 Forearm.seek 2 + 800 counts @14:23:39.46 SE.reconfigure :pick @14:23:39.51 SE.seek :beamBreak @14:23:41.42 SE.configure :default @14:23:42.03 Elbow.seek 2 @14:23:42.22 Forearm.seek 2 + 300 counts @14:23:42.39 Forearm.reconfigure :loose @14:23:42.44 Hand.close @14:23:43.14 SE.seek :down @14:23:43.36
Forearm.seek :retract @14:23:44.33 Elbow.seek PC @14:23:44.90 SE.stop @14:23:46.82
Elbow.reconfigure :loose @14:23:46.93 Forearm.reconfigure :tight @14:23:46.98 Forearm.seek PC @14:23:48.39 PC.seek :closed @14:23:52.81 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.59 @14:23:52.82 Hand.open @14:23:53.57 Forearm.reconfigure :loose @14:23:53.62 Forearm.seek :retract @14:23:54.62 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @14:23:54.64 PH.seek 28C,5:00 @14:24:38.41 PH.hold 2:00:00,28C @14:24:38.46 delay 1:30 @14:26:08.50 PSR.select :air @14:26:08.52 PSV.dial PSR @14:26:09.54 Pulling 0.5ml of PSR.wash @14:26:09.55 PSR.select :wash @14:26:09.67 PS.seek 0.5ml @14:26:15.65 delay 5 seconds @14:26:20.67 PSR.select :air @14:26:20.79 PS.seek 2ml @14:26:36.90 PTV.dial :puck @14:26:37.64 PSV.dial PTV @14:26:38.39 PBV.dial :waste1 @14:26:39.81 PS.seek 1.5ml @14:26:45.72 delay 3 seconds @14:26:48.73 PBV.dial between PSV and waste1 @14:26:49.11 PS.seek 0ml @14:27:05.25 delay 5:00 @14:32:05.26 recoverPSRreagent08 @14:32:05.27 PTV.dial :puck @14:32:05.35 PBV.dial :waste1 @14:32:05.74 delay 30 seconds @14:32:35.77 PBV.dial between PSV and waste1 @14:32:36.15 PSR.select :air @14:32:36.17 PSV.dial PSR @14:32:36.90 PS.reconfigure :fast @14:32:36.95 PS.seek 2.5ml @14:33:00.24 PSV.dial PTV @14:33:00.98 delay 5 seconds @14:33:06.37 PSV.dial PSR @14:33:07.18 delay 5 seconds @14:33:12.20 PSV.dial PTV @14:33:12.93 delay 5 seconds @14:33:17.95 PSV.dial :air @14:33:18.70 PS.seek 0ml @14:33:41.84 PTV.dial :PRVmixing,via: PRV @14:33:43.13 PSV.dial :mixloop @14:33:43.58 PRV.dial :ab1 @14:33:44.33 PS.reconfigure :slow @14:33:44.38 PS.seek 0.15ml @14:33:47.90 delay 2 seconds @14:33:49.92 CBV.dial PRV @14:33:49.94
PRV.dial CBV @14:33:50.52 PS.seek 0.34ml @14:33:54.93 delay 2 seconds @14:33:56.95 CBV.dial between PRV and PSV @14:33:57.27 PRV.dial :air @14:33:57.73 PS.seek 0.9ml @14:34:09.65 PRV.dial :waste2 @14:34:10.39 PS.seek 0.5ml @14:34:19.05 addPMLReagent08 @14:34:19.06 PSV.dial :air @14:34:19.51 PS.reconfigure :default @14:34:19.56 PS.seek 2ml @14:34:35.68 PSV.dial :PML @14:34:36.13 PTV.dial :mixing @14:34:36.88 PBV.dial :waste1 @14:34:37.32 PS.seek 1.85ml @14:34:39.65 PBV.dial between PSV and waste1 @14:34:40.03 PS.seek 0ml @14:34:59.77 delay 2 seconds @14:35:01.79 Beginning samplePassC @14:35:01.80 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:35:01.99 Elbow.reconfigure :tight @14:35:02.04 Elbow.seek FlushPuck::Garage @14:35:03.40 Elbow.reconfigure :loose @14:35:03.50 Forearm.seek :garage @14:35:04.57 Hand.close @14:35:05.49 Forearm.seek :retract @14:35:06.56 Elbow.reconfigure :tight @14:35:07.03 Elbow.seek CC @14:35:09.85 Elbow.reconfigure :loose @14:35:09.95 Forearm.reconfigure :tight @14:35:10.00 Forearm.seek CC @14:35:11.35 CC.seek :closed @14:35:15.33 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.99 @14:35:15.39 Hand.open @14:35:16.18 Forearm.reconfigure :loose @14:35:16.23 Forearm.seek :retract @14:35:17.32 PSV.dial PSR @14:35:18.35 Pulling 1.5ml of PSR.flush @14:35:18.36 PSR.select :flush @14:35:18.48 PS.seek 1.5ml @14:35:34.58 delay 5 seconds @14:35:39.60 PSV.dial :waste1 @14:35:40.06 PS.seek 0ml @14:35:56.24 PSV.dial PSR @14:35:56.69 PSR.select :air @14:35:56.81 PS.seek 2.5ml @14:36:23.12 PRV.dial CBV,avoiding: :ab2 @14:36:23.14
CBV.dial PRV @14:36:24.77 PSV.dial :mixloop @14:36:25.79 CTV.dial :air,avoiding: :intake @14:36:25.86 PTV.dial :PRVmixing @14:36:26.62 PS.seek 0ml @14:36:53.18 PSV.dial :air @14:36:53.64 PS.seek 2.5ml @14:37:20.05 PSV.dial :mixloop @14:37:20.51 PS.seek 0ml @14:37:46.89 PSV.dial :air @14:37:47.40 PS.seek 2.5ml @14:38:14.30 PSV.dial :mixloop @14:38:14.76 PS.seek 0ml @14:38:41.11 Garaging FlushPuck in Collection Clamp @14:38:41.19 Elbow.reconfigure :tight @14:38:41.24 Elbow.seek CC @14:38:41.36 Elbow.reconfigure :loose @14:38:41.47 Forearm.seek CC @14:38:42.40 Hand.close @14:38:43.12 CC.seek :open @14:38:47.21 Forearm.seek :retract @14:38:48.19 Elbow.reconfigure :tight @14:38:48.24 Elbow.seek :garage @14:38:51.07 Elbow.reconfigure :loose @14:38:51.18 Forearm.reconfigure :tight @14:38:51.23 Forearm.seek :garage @14:38:52.35 Hand.open @14:38:53.17 Forearm.reconfigure :loose @14:38:53.22 Forearm.seek :retract @14:38:54.22 delayUntil "14:43:01.78" @14:38:54.25 Whole Cell Archive sampling 1000ml @14:38:54.27 Loading wcr puck from tube 2 into Collection Clamp @14:38:54.51 SC.reconfigure :tight @14:38:54.57 SC.seek 2 @14:38:54.68 SC.configure :limp @14:38:54.91 Elbow.reconfigure :tight @14:38:54.96 Elbow.seek 2 @14:38:56.84 Forearm.reconfigure :tight @14:38:56.90 Forearm.seek 2 + 800 counts @14:38:57.94 SE.reconfigure :pick @14:38:58.00 SE.seek :beamBreak @14:39:00.51 SE.configure :default @14:39:00.68 Elbow.seek 2 @14:39:00.87 Forearm.seek 2 + 300 counts @14:39:01.13 Forearm.reconfigure :loose @14:39:01.18 Hand.close @14:39:01.87 SE.seek :down @14:39:02.08 Forearm.seek :retract @14:39:03.08 Elbow.seek CC @14:39:03.70 SE.stop @14:39:04.64 Elbow.reconfigure :loose @14:39:04.75 Forearm.reconfigure :tight @14:39:04.80 Forearm.seek CC @14:39:06.14 CC.seek :closed @14:39:10.14 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.95 @14:39:10.15 Hand.open @14:39:10.92 Forearm.reconfigure :loose @14:39:10.97 Forearm.seek :retract @14:39:12.04 collectSample 1000ml w/bubble point of 35psi @14:39:12.05 Priming 7ml @14:39:12.06 Intake.select :close @14:39:12.07 Exhaust.select :close @14:39:12.08 SS.hold @14:39:12.29 Calibrating pressure sensors @14:39:12.30 CTV.dial :air @14:39:12.32 SSV.dial :spit,avoiding: :exhaust @14:39:12.34 SS.reconfigure :fast @14:39:12.39 SS.seek 5ml @14:39:15.28 SSV.dial CBV @14:39:15.30 CBV.dial SSV @14:39:16.69 delay 3.8 seconds @14:39:20.63 Sensor limits: Intake max 114.1psia; Outlet max 114.7psia @14:39:20.64 SSV.dial :spit @14:39:21.97 SS.seek 0ml @14:39:24.86 SSV.dial CBV @14:39:26.20 CTV.dial :intake @14:39:26.98 SSV.dial CBV @14:39:27.01 CBV.dial SSV @14:39:27.16 Allowing 40s for 7.0ml of remaining 7.0ml @14:39:27.18 Intake.select :open @14:39:29.35 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:39:29.37 SS.reconfigure :inhaling 2.17ml/s @14:39:29.41 SS.seek 7ml @14:39:34.73 Stroke completed -- waiting for pressure to equalize ... @14:39:34.74 SS.reconfigure :exhale @14:39:34.79 SS.hold @14:39:51.14 Intake.select :close @14:39:51.31 SSV.dial :exhaust @14:39:52.07 Exhaust.select :open @14:39:52.19 SS.seek 0ml @14:39:56.01 Exhaust.select :close @14:39:56.13 Sampling 1000.0ml @14:39:56.14 Intake.select :close @14:39:56.15 Exhaust.select :close @14:39:56.17 SS.reconfigure :default @14:39:56.21 SS.hold @14:39:56.42 CTV.dial :intake @14:39:56.61 SSV.dial CBV @14:39:56.65 CBV.dial SSV @14:39:57.40 Allowing 130s for 25.0ml of remaining 1000.0ml @14:39:57.42 Intake.select :open @14:39:59.59 Intake @14.2psia, vacuum from 8.7 to 11.7psi @14:39:59.61 SS.reconfigure :inhaling 2.17ml/s @14:39:59.66 SS.seek 25ml @14:40:06.28 Vacuum too high -- waiting up to 40 seconds @14:40:06.30 SS.reconfigure :exhale @14:40:06.35 SS.hold @14:40:29.23 Intake @13.4psia, vacuum from 7.9 to 10.9psi @14:40:29.25 SS.reconfigure :inhaling 1.95ml/s @14:40:29.30 SS.seek 25ml @14:40:32.69 Vacuum too high -- waiting up to 40 seconds @14:40:32.71 SS.reconfigure :exhale @14:40:32.76 SS.hold @14:40:53.56 Intake @13.4psia, vacuum from 7.9 to 10.9psi @14:40:53.58 SS.reconfigure :inhaling 1.77ml/s @14:40:53.63 SS.seek 25ml @14:40:57.47 Vacuum too high -- waiting up to 40 seconds @14:40:57.49 SS.reconfigure :exhale @14:40:57.54 SS.hold @14:41:20.77 Intake @13.4psia, vacuum from 7.9 to 10.9psi @14:41:20.79 SS.reconfigure :inhaling 1.59ml/s @14:41:20.84 SS.seek 25ml @14:41:25.16 Vacuum too high -- waiting up to 40 seconds @14:41:25.17 SS.reconfigure :exhale @14:41:25.23 SS.hold @14:41:48.06 Intake @13.4psia, vacuum from 7.9 to 10.9psi @14:41:48.08 SS.reconfigure :inhaling 1.43ml/s @14:41:48.13 SS.seek 25ml @14:41:50.01 Stroke completed -- waiting for pressure to equalize ... @14:41:50.02 SS.reconfigure :exhale @14:41:50.07 SS.hold @14:41:59.77 Intake.select :close @14:42:00.31 SSV.dial :exhaust @14:42:01.15 Exhaust.select :open @14:42:01.27 SS.seek 0ml @14:42:10.60 Exhaust.select :close @14:42:10.72 SSV.dial CBV @14:42:10.74 CBV.dial SSV @14:42:11.53 Allowing 130s for 25.0ml of remaining 975.0ml @14:42:11.54 Intake.select :open @14:42:13.71 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:42:13.73 SS.reconfigure :inhaling 1.43ml/s @14:42:13.78 SS.seek 25ml @14:42:20.69 Vacuum too high -- waiting up to 40 seconds @14:42:20.70 SS.reconfigure :exhale @14:42:20.76 SS.hold @14:42:39.79 Intake @13.6psia, vacuum from 8.1 to 11.1psi @14:42:39.81 SS.reconfigure :inhaling 1.29ml/s @14:42:39.86 SS.seek 25ml @14:42:44.63 Vacuum too high -- waiting up to 40 seconds @14:42:44.64 SS.reconfigure :exhale @14:42:44.70 SS.hold @14:43:01.79 recoverPRVreagent08 @14:43:01.80 PTV.dial :mixing @14:43:02.55 PBV.dial :waste1 @14:43:02.94 delay 30 seconds @14:43:09.44 SS.reconfigure :inhaling 1.16ml/s @14:43:09.50 SS.seek 25ml @14:43:15.75 Vacuum too high -- waiting up to 40 seconds @14:43:15.77 SS.reconfigure :exhale @14:43:15.82 SS.hold @14:43:32.95 PBV.dial between PSV and waste1 @14:43:33.33 PSV.dial :air @14:43:34.22 PS.reconfigure :fast @14:43:34.31 PS.seek 2.5ml @14:43:38.65 Intake @13.7psia, vacuum from 8.2 to 11.2psi @14:43:38.67 SS.reconfigure :inhaling 1.04ml/s @14:43:38.72 SS.seek 25ml @14:43:44.65 Vacuum too high -- waiting up to 40 seconds @14:43:44.67 SS.reconfigure :exhale @14:43:44.72 SS.hold @14:43:57.98 PSV.dial :mixloop @14:43:58.45 delay 2 seconds @14:44:00.47 PSV.dial :air @14:44:00.95 delay 2 seconds @14:44:03.39 PSV.dial :mixloop @14:44:03.86 delay 2 seconds @14:44:05.87 PSV.dial :air @14:44:06.36 PS.seek 0ml @14:44:07.77 Intake @13.8psia, vacuum from 8.3 to 11.3psi @14:44:07.79 SS.reconfigure :inhaling 0.94ml/s @14:44:07.83 SS.seek 25ml @14:44:09.21 Stroke completed -- waiting for pressure to equalize ... @14:44:09.22 SS.reconfigure :exhale @14:44:09.27 SS.hold @14:44:21.55 SS.reconfigure :default @14:44:21.61 SS.hold @14:44:21.68 Intake.select :close @14:44:22.31 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 50.0ml @14:44:22.32 SSV.dial :exhaust @14:44:23.10 Exhaust.select :open @14:44:23.22 SS.reconfigure :exhale @14:44:23.27 SS.seek 0ml @14:44:29.77 washCycleDA #1 of 2 @14:44:29.78 PSR.select :air @14:44:29.80 PSV.dial PSR @14:44:31.11 Pulling 0.5ml of PSR.wash @14:44:31.12 PSR.select :wash @14:44:31.24 PS.reconfigure :default @14:44:31.29 PS.seek 0.5ml @14:44:32.55 Exhaust.select :close @14:44:32.67 Sampled 50.0ml @14:44:32.69 Drying puck before fixing for 4:00 @14:44:32.70 CBV.dial :waste2b @14:44:33.45 delay 1:00 @14:44:37.30 delay 5 seconds @14:44:42.32 PSR.select :air @14:44:42.44 PS.seek 2ml @14:44:58.61 PTV.dial :puck @14:44:59.36 PSV.dial PTV @14:45:00.09 PBV.dial :waste1 @14:45:00.53 PS.seek 1.5ml @14:45:06.42 delay 3 seconds @14:45:09.44 PBV.dial between PSV and waste1 @14:45:09.82 PS.seek 0ml @14:45:25.92 delay 2:00 @14:45:33.47 CSV.dial :air @14:45:33.94 CS.reconfigure :fast @14:45:33.99 CS.seek 10ml @14:45:57.53 Pumping wcr puck from tube 2 up to 35psi... @14:45:57.54 CBV.dial SSV @14:45:58.79 CBV.dial between CSV and waste2b @14:45:59.75 CSV.dial between air and CTV @14:46:00.08 CS.seek 7.249ml @14:46:07.22 CTV.dial CSV,avoiding: :air @14:46:07.24 CSV.dial CTV @14:46:08.08 CS.reconfigure :pressurize @14:46:08.13 CS.seek 0ml @14:46:26.39 CBV.dial :waste2b @14:46:26.71 CS.reconfigure :default @14:46:26.77 CS.seek 0ml @14:46:28.27 delay 1:00 @14:47:25.95 recoverPSRreagent08 @14:47:25.96 PTV.dial :puck @14:47:26.03 PBV.dial :waste1 @14:47:26.85 delay 30 seconds @14:47:28.29 CTV.dial :air,avoiding: :intake @14:47:29.67 Fix #1 of 1 (with RNAlater) for 4:00 @14:47:29.68 CSV.dial CSR @14:47:30.12 Pulling 2ml of CSR.RNAlater @14:47:30.13 CSR.select :RNAlater @14:47:30.25 CS.seek 2ml @14:47:36.21 delay 5 seconds @14:47:41.23 CSR.select :air @14:47:41.35 CS.reconfigure :fast @14:47:41.40 CS.seek 10ml @14:47:56.88 PBV.dial between PSV and waste1 @14:47:57.26 PSR.select :air @14:47:57.28 PSV.dial PSR @14:47:58.03 PS.reconfigure :fast @14:47:58.08 PS.seek 2.5ml @14:48:00.43 Pumping wcr puck from tube 2 up to 35psi... @14:48:00.45 CBV.dial SSV @14:48:01.68 CBV.dial between CSV and waste2b @14:48:02.65 CSV.dial between air and CTV @14:48:03.48 CS.seek 7.132ml @14:48:11.00 CTV.dial CSV,avoiding: :air @14:48:11.02 CSV.dial CTV @14:48:12.43 CS.reconfigure :pressurize @14:48:12.49 CS.seek 0ml @14:48:21.60 PSV.dial PTV @14:48:22.36 delay 5 seconds @14:48:27.38 PSV.dial PSR @14:48:28.11 delay 5 seconds @14:48:28.29 delay 4:00 @14:48:33.13 PSV.dial PTV @14:48:33.87 delay 5 seconds @14:48:38.90 PSV.dial :air @14:48:39.64 PS.seek 0ml @14:49:02.92 washCycleDA #2 of 2 @14:49:02.93 PSR.select :air @14:49:02.95 PSV.dial PSR @14:49:04.26 Pulling 0.5ml of PSR.wash @14:49:04.27 PSR.select :wash @14:49:04.39 PS.reconfigure :default @14:49:04.44 PS.seek 0.5ml @14:49:10.45 delay 5 seconds @14:49:15.47 PSR.select :air @14:49:15.59 PS.seek 2ml @14:49:31.79 PTV.dial :puck @14:49:31.87 PSV.dial PTV @14:49:32.62 PBV.dial :waste1 @14:49:33.08 PS.seek 1.5ml @14:49:38.98 delay 3 seconds @14:49:42.00 PBV.dial between PSV and waste1 @14:49:42.85 PS.seek 0ml @14:49:59.04 delay 2:00 @14:51:59.07 recoverPSRreagent08 @14:51:59.08 PTV.dial :puck @14:51:59.15 PBV.dial :waste1 @14:51:59.54 delay 30 seconds @14:52:28.32 CBV.dial :waste2b @14:52:28.68 CS.reconfigure :default @14:52:28.73 CS.seek 0ml @14:52:29.56 PBV.dial between PSV and waste1 @14:52:29.93 PSR.select :air @14:52:29.95 PSV.dial PSR @14:52:30.81 PS.reconfigure :fast @14:52:30.86 PS.seek 2.5ml @14:52:32.46 delay 1:00 @14:52:54.16 PSV.dial PTV @14:52:54.90 delay 5 seconds @14:52:59.92 PSV.dial PSR @14:53:00.77 delay 5 seconds @14:53:05.80 PSV.dial PTV @14:53:06.54 delay 5 seconds @14:53:11.56 PSV.dial :air @14:53:12.31 PS.seek 0ml @14:53:32.48 CTV.dial :air,avoiding: :intake @14:53:35.73 getPMLReagent08 @14:53:35.74 PRV.dial :air @14:53:36.30 delay 5 seconds @14:53:41.33 PTV.dial :PRVmixing @14:53:42.63 delay 5 seconds @14:53:47.65 PRV.dial :ab2 @14:53:48.12 PTV.dial :PRVmixing @14:53:48.30 PSV.dial :mixloop @14:53:48.76 PS.reconfigure :default @14:53:48.81 PS.seek 0.3ml @14:53:52.73 delay 2 seconds @14:53:54.75 PRV.dial :air @14:53:55.27 PS.seek 0.55ml @14:53:58.63 addPMLReagent08 @14:53:58.64 PSV.dial :air @14:53:59.09 PS.seek 2ml @14:54:14.66 PSV.dial :PML @14:54:15.12 PTV.dial :mixing @14:54:15.87 PBV.dial :waste1 @14:54:16.30 PS.seek 1.85ml @14:54:18.63 PBV.dial between PSV and waste1 @14:54:19.01 PS.seek 0ml @14:54:38.69 delay 2 seconds @14:54:40.71 delay 8:00 @15:02:40.74 recoverPRVreagent08 @15:02:40.75 PTV.dial :mixing @15:02:40.94 PBV.dial :waste1 @15:02:41.33 delay 30 seconds @15:03:11.35 PBV.dial between PSV and waste1 @15:03:11.75 PSV.dial :air @15:03:12.21 PS.reconfigure :fast @15:03:12.26 PS.seek 2.5ml @15:03:35.75 PSV.dial :mixloop @15:03:36.28 delay 2 seconds @15:03:38.30 PSV.dial :air @15:03:38.75 delay 2 seconds @15:03:40.77 PSV.dial :mixloop @15:03:41.23 delay 2 seconds @15:03:43.25 PSV.dial :air @15:03:43.71 PS.seek 0ml @15:04:06.97 washCycleDA #1 of 3 @15:04:06.98 PSR.select :air @15:04:07.00 PSV.dial PSR @15:04:08.31 Pulling 0.5ml of PSR.wash @15:04:08.32 PSR.select :wash @15:04:08.44 PS.reconfigure :default @15:04:08.49 PS.seek 0.5ml @15:04:14.37 delay 5 seconds @15:04:19.39 PSR.select :air @15:04:19.52 PS.seek 2ml @15:04:35.65 PTV.dial :puck @15:04:36.40 PSV.dial PTV @15:04:37.14 PBV.dial :waste1 @15:04:37.59 PS.seek 1.5ml @15:04:43.53 delay 3 seconds @15:04:46.55 PBV.dial between PSV and waste1 @15:04:46.94 PS.seek 0ml @15:05:03.12 delay 2:00 @15:07:03.15 recoverPSRreagent08 @15:07:03.16 PTV.dial :puck @15:07:03.23 PBV.dial :waste1 @15:07:03.62 delay 30 seconds @15:07:33.65 PBV.dial between PSV and waste1 @15:07:34.03 PSR.select :air @15:07:34.05 PSV.dial PSR @15:07:34.91 PS.reconfigure :fast @15:07:34.96 PS.seek 2.5ml @15:07:58.28 PSV.dial PTV @15:07:59.01 delay 5 seconds @15:08:04.03 PSV.dial PSR @15:08:04.89 delay 5 seconds @15:08:09.91 PSV.dial PTV @15:08:10.64 delay 5 seconds @15:08:15.66 PSV.dial :air @15:08:16.42 PS.seek 0ml @15:08:39.81 washCycleDA #2 of 3 @15:08:39.82 PSR.select :air @15:08:39.84 PSV.dial PSR @15:08:41.15 Pulling 0.5ml of PSR.wash @15:08:41.16 PSR.select :wash @15:08:41.28 PS.reconfigure :default @15:08:41.33 PS.seek 0.5ml @15:08:47.31 delay 5 seconds @15:08:52.33 PSR.select :air @15:08:52.45 PS.seek 2ml @15:09:08.59 PTV.dial :puck @15:09:08.67 PSV.dial PTV @15:09:09.40 PBV.dial :waste1 @15:09:09.84 PS.seek 1.5ml @15:09:15.84 delay 3 seconds @15:09:18.86 PBV.dial between PSV and waste1 @15:09:19.24 PS.seek 0ml @15:09:35.40 delay 2:00 @15:11:35.43 recoverPSRreagent08 @15:11:35.44 PTV.dial :puck @15:11:35.51 PBV.dial :waste1 @15:11:36.37 delay 30 seconds @15:12:06.40 PBV.dial between PSV and waste1 @15:12:06.78 PSR.select :air @15:12:06.80 PSV.dial PSR @15:12:07.55 PS.reconfigure :fast @15:12:07.60 PS.seek 2.5ml @15:12:31.05 PSV.dial PTV @15:12:31.81 delay 5 seconds @15:12:36.82 PSV.dial PSR @15:12:37.56 delay 5 seconds @15:12:42.58 PSV.dial PTV @15:12:43.34 delay 5 seconds @15:12:48.36 PSV.dial :air @15:12:49.11 PS.seek 0ml @15:13:12.40 washCycleDA #3 of 3 @15:13:12.41 PSR.select :air @15:13:12.43 PSV.dial PSR @15:13:13.73 Pulling 0.5ml of PSR.wash @15:13:13.74 PSR.select :wash @15:13:13.85 PS.reconfigure :default @15:13:13.90 PS.seek 0.5ml @15:13:19.84 delay 5 seconds @15:13:24.86 PSR.select :air @15:13:24.98 PS.seek 2ml @15:13:41.15 PTV.dial :puck @15:13:41.23 PSV.dial PTV @15:13:41.96 PBV.dial :waste1 @15:13:42.40 PS.seek 1.5ml @15:13:48.27 delay 3 seconds @15:13:51.29 PBV.dial between PSV and waste1 @15:13:51.67 PS.seek 0ml @15:14:08.02 delay 2:00 @15:16:08.05 recoverPSRreagent08 @15:16:08.06 PTV.dial :puck @15:16:08.15 PBV.dial :waste1 @15:16:08.52 delay 30 seconds @15:16:38.55 PBV.dial between PSV and waste1 @15:16:38.93 PSR.select :air @15:16:38.95 PSV.dial PSR @15:16:39.81 PS.reconfigure :fast @15:16:39.86 PS.seek 2.5ml @15:17:03.30 PSV.dial PTV @15:17:04.04 delay 5 seconds @15:17:09.06 PSV.dial PSR @15:17:09.79 delay 5 seconds @15:17:14.81 PSV.dial PTV @15:17:15.55 delay 5 seconds @15:17:20.57 PSV.dial :air @15:17:21.32 PS.seek 0ml @15:17:44.55 Gate.power :camera,:ON @15:17:44.57 Mixing DA Substrates PSR.select :air @15:17:44.59 PSV.dial PSR @15:17:45.96 Pulling 0.25ml of PSR.sub1 @15:17:45.99 PSR.select :sub1 @15:17:46.18 PS.reconfigure :default @15:17:46.29 PS.seek 0.25ml @15:17:49.78 delay 5 seconds @15:17:54.80 Pulling 0.25ml of PSR.sub2 @15:17:54.81 PSR.select :sub2 @15:17:54.93 PS.seek 0.5ml @15:17:58.33 delay 5 seconds @15:18:03.35 PSR.select :air @15:18:03.47 PS.seek 2ml @15:18:19.64 PTV.dial :puck @15:18:20.15 PSV.dial PTV @15:18:20.96 PBV.dial :waste1 @15:18:21.41 PS.seek 1.5ml @15:18:27.39 delay 3 seconds @15:18:30.41 PBV.dial between PSV and waste1 @15:18:30.81 PS.seek 0ml @15:18:46.99 delay 10 seconds @15:18:57.02 recoverPSRreagent08 @15:18:57.03 PTV.dial :puck @15:18:57.10 PBV.dial :waste1 @15:18:57.49 delay 30 seconds @15:19:27.52 PBV.dial between PSV and waste1 @15:19:27.91 PSR.select :air @15:19:27.93 PSV.dial PSR @15:19:28.79 PS.reconfigure :fast @15:19:28.84 PS.seek 2.5ml @15:19:52.33 PSV.dial PTV @15:19:53.07 delay 5 seconds @15:19:58.09 PSV.dial PSR @15:19:58.94 delay 5 seconds @15:20:03.96 PSV.dial PTV @15:20:04.69 delay 5 seconds @15:20:09.71 PSV.dial :air @15:20:10.49 PS.seek 0ml @15:20:33.86 Additional air clear @15:20:33.87 PSR.select :air @15:20:33.89 PSV.dial PSR @15:20:35.21 Pulling 2ml of PSR.air @15:20:35.22 PSR.select :air @15:20:35.24 PS.reconfigure :default @15:20:35.29 PS.seek 2ml @15:20:56.58 delay 5 seconds @15:21:01.60 PTV.dial :puck @15:21:01.69 PSV.dial PTV @15:21:02.42 PBV.dial :waste1 @15:21:02.87 PS.seek 1.5ml @15:21:08.83 delay 3 seconds @15:21:11.85 PBV.dial between PSV and waste1 @15:21:12.24 delay 1 second @15:21:13.26 PSV.dial :waste1 @15:21:14.29 PS.seek 0ml @15:21:30.66 Processing Puck Heater at 28C before imaging @15:21:30.67 PH.coast @15:21:30.79 Imaging da puck in Processing Clamp while storing it to tube 1 @15:21:30.97 SC.reconfigure :tight @15:21:31.02 SC.seek 1 @15:21:34.63 SC.configure :limp @15:21:34.84 Elbow.reconfigure :tight @15:21:34.90 Elbow.seek 1 @15:21:36.73 Forearm.reconfigure :tight @15:21:36.78 Forearm.seek 1 + 800 counts @15:21:37.91 SE.reconfigure :pick @15:21:38.47 SE.seek :beamBreak @15:21:40.55 SE.configure :default @15:21:40.72 Elbow.reconfigure :limp @15:21:40.77 Forearm.reconfigure :loose @15:21:40.82 Forearm.seek 1 @15:21:41.18 Elbow.hold @15:21:41.25 Elbow.reconfigure :tight @15:21:41.36 SE.seek 9.1 Pucks below Top Plate @15:21:41.89 Forearm.force -20 @15:21:41.95 Forearm.seek 1 + 308 counts @15:21:42.30 Forearm.reconfigure :tight @15:21:42.35 Forearm.seek 1 + 800 counts @15:21:42.65 SE.seek 10.1 Pucks below Top Plate @15:21:43.26 Forearm.reconfigure :loose @15:21:43.31 Forearm.seek :retract @15:21:44.21 Elbow.seek PC @15:21:46.78 Elbow.reconfigure :loose @15:21:46.89 Forearm.seek PC @15:21:47.97 Hand.close @15:21:48.69 PC.seek :open @15:21:53.25 Forearm.seek :retract @15:21:54.27 Elbow.reconfigure :tight @15:21:54.32 Elbow.seek 1 @15:21:56.97 Forearm.reconfigure :tight @15:21:57.02 Forearm.seek 1 + 800 counts @15:21:57.99 Hand.open @15:21:58.76 SE.seek 9.86 Pucks below Top Plate @15:21:59.05 SE.reconfigure :seal @15:21:59.10 SE.seek 9.2 Pucks below Top Plate @15:21:59.63 Lights, camera, ACTION! @15:21:59.66 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da25sep2315h625ml.tif @15:22:19.16 Waiting >20 seconds for da to relinquish FlushPuck @15:22:20.05 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 17.106 seconds /var/log/esp/da25sep2315h625ml.tif: TIFF Upload Complete @15:22:20.07 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da25sep2315h625ml15s.tif @15:22:35.68 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da25sep2315h625ml15s.tif: TIFF Upload Complete @15:22:35.71 PuckCamera: snap -bin=2,2 21.0 /var/log/esp/midres/da25sep2315h625ml21s.tif @15:22:57.32 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21 seconds /var/log/esp/midres/da25sep2315h625ml21s.tif: TIFF Upload Complete @15:22:57.34 Gate.power :camera,:OFF @15:22:57.35 DAfans.email "17.106 sec auto-exposure",Subject: "da25sep2315h625ml images!" @15:22:58.38 Elbow.seek 1 @15:22:58.58 Forearm.seek 1 + 800 counts @15:22:58.67 SE.reconfigure :gently @15:22:58.73 SE.seek 9.92 Pucks below Top Plate @15:22:59.50 Elbow.reconfigure :limp @15:22:59.55 Forearm.reconfigure :loose @15:22:59.62 Forearm.seek 1 @15:23:00.02 Elbow.hold @15:23:00.09 Elbow.reconfigure :tight @15:23:00.14 SE.reconfigure :default @15:23:00.20 SE.seek 10.1 Pucks below Top Plate @15:23:00.65 Forearm.force -20 @15:23:00.81 Forearm.seek 1 + 306 counts @15:23:01.32 Forearm.reconfigure :tight @15:23:01.38 Forearm.seek 1 + 800 counts @15:23:01.72 SE.seek :down @15:23:02.99 Forearm.reconfigure :loose @15:23:03.09 Forearm.seek :retract @15:23:03.53 SE.stop @15:23:04.22 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:23:04.76 Elbow.seek FlushPuck::Garage @15:23:06.68 Elbow.reconfigure :loose @15:23:06.89 Forearm.seek :garage @15:23:07.91 Hand.close @15:23:08.97 Forearm.seek :retract @15:23:11.10 Elbow.reconfigure :tight @15:23:11.20 Elbow.seek PC @15:23:12.63 Elbow.reconfigure :loose @15:23:12.84 Forearm.reconfigure :tight @15:23:12.93 Forearm.seek PC @15:23:14.67 PC.seek :closed @15:23:19.24 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.61 @15:23:19.38 Hand.open @15:23:20.34 Forearm.reconfigure :loose @15:23:20.44 Forearm.seek :retract @15:23:21.55 PSR.select :air @15:23:21.61 Storing wcr puck in Collection Clamp to tube 1 @15:23:21.65 PSV.dial PSR @15:23:22.35 Pulling 0.5ml of PSR.flush @15:23:22.42 PSR.select :flush @15:23:22.85 PS.seek 0.5ml @15:23:22.99 Elbow.reconfigure :tight @15:23:23.09 Elbow.seek 1 @15:23:25.94 Forearm.reconfigure :tight @15:23:26.05 Forearm.seek 1 + 800 counts @15:23:27.36 SE.reconfigure :pick @15:23:27.48 SE.seek :beamBreak @15:23:28.86 delay 5 seconds @15:23:29.65 SE.configure :default @15:23:31.03 Elbow.reconfigure :limp @15:23:31.18 Forearm.reconfigure :loose @15:23:31.35 Forearm.seek 1 @15:23:31.80 Elbow.hold @15:23:31.95 Elbow.reconfigure :tight @15:23:32.19 SE.seek 10.11 Pucks below Top Plate @15:23:32.97 Forearm.force -20 @15:23:33.07 Forearm.seek 1 + 317 counts @15:23:33.61 Forearm.reconfigure :tight @15:23:33.73 Forearm.seek 1 + 800 counts @15:23:33.88 PSR.select :air @15:23:34.05 PS.seek 2.5ml @15:23:34.19 SE.seek 11.11 Pucks below Top Plate @15:23:34.96 Forearm.reconfigure :loose @15:23:35.08 Forearm.seek :retract @15:23:35.85 upsync @15:23:36.04 Elbow.seek CC @15:23:37.69 Elbow.reconfigure :loose @15:23:37.80 Forearm.seek CC @15:23:38.75 Hand.close @15:23:39.44 CC.seek :open @15:23:43.67 Forearm.seek :retract @15:23:44.65 Elbow.reconfigure :tight @15:23:44.70 Elbow.seek 1 @15:23:46.47 Elbow.reconfigure :limp @15:23:46.52 Forearm.seek 1 @15:23:47.64 Elbow.hold @15:23:48.11 Elbow.reconfigure :tight @15:23:48.22 Hand.open @15:23:49.07 Forearm.force -20 @15:23:49.12 Forearm.seek 1 + 318 counts @15:23:49.49 Forearm.reconfigure :tight @15:23:49.54 Forearm.seek 1 + 800 counts @15:23:49.92 SE.seek :down @15:23:51.05 Forearm.reconfigure :loose @15:23:51.10 Forearm.seek :retract @15:23:51.59 SE.stop @15:23:52.01
Starting finalPurge @15:23:55.42 delay 1 second @15:23:56.44 PRV.dial :waste2,avoiding: :ab2 @15:23:58.30 PTV.dial :PRVmixing @15:23:59.59 PSV.dial :mixloop @15:24:00.62 PS.seek :empty @15:24:11.65
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:24:27.05 PSR.select :air @15:24:27.07 PSV.dial PSR @15:24:28.09 Pulling 0.5ml of PSR.flush @15:24:28.11 PSR.select :flush @15:24:28.22 PS.seek 0.5ml @15:24:34.17 delay 5 seconds @15:24:39.19 PSR.select :air @15:24:39.31 PS.seek 2.5ml @15:25:00.55 delay 1 second @15:25:01.57 PBV.dial :waste1 @15:25:01.95 PTV.dial :mixing @15:25:02.70 PSV.dial :mixloop @15:25:03.74 PS.seek :empty @15:25:30.14 PSV.dial PSR @15:25:31.17 PSR.select :flush @15:25:31.29 PS.seek 1ml @15:25:42.30 PSR.select :air @15:25:42.41 PS.seek 2.5ml @15:25:58.64 PSV.dial PTV @15:25:59.39 PTV.dial :puck @15:26:00.12 PBV.dial :waste1 @15:26:00.21 PS.seek 0ml @15:26:26.58 PSV.dial PSR @15:26:27.31 PSR.select :flush @15:26:27.89 PS.seek 0.5ml @15:26:33.76 PSR.select :air @15:26:33.88 PS.seek 2.5ml @15:26:55.14 PSV.dial PTV @15:26:55.87 PBV.dial PSV @15:26:56.46 PS.seek 0ml @15:27:22.79 PSV.dial PBV @15:27:23.25 PS.seek 2.5ml @15:27:49.57 PSV.dial :waste1 @15:27:50.32 PS.seek 0ml @15:28:16.70 PSV.dial PSR @15:28:17.28 PSR.select :air @15:28:17.30 PS.seek 2.5ml @15:28:43.64 PSV.dial PTV @15:28:44.39 PBV.dial PSV @15:28:44.47 PS.seek 0ml @15:29:10.82 PSV.dial PBV @15:29:11.46 PS.seek 2.5ml @15:29:37.89 PSV.dial :waste1 @15:29:38.63 PS.seek 0ml @15:30:04.98 PSV.dial PSR @15:30:05.43 PSR.select :flush @15:30:05.55 PS.seek 1ml @15:30:16.54 PSR.select :air @15:30:16.66 PS.seek 2.5ml @15:30:32.82 PSV.dial PTV @15:30:33.54 PTV.dial :puck @15:30:33.62 PBV.dial :waste1 @15:30:34.20 PS.seek 0ml @15:31:00.54 PSV.dial PSR @15:31:01.28 PSR.select :flush @15:31:01.40 PS.seek 0.5ml @15:31:07.38 PSR.select :air @15:31:07.50 PS.seek 2.5ml @15:31:28.73 PSV.dial PTV @15:31:29.46 PBV.dial PSV @15:31:30.05 PS.seek 0ml @15:31:56.35 PSV.dial PBV @15:31:56.81 PS.seek 2.5ml @15:32:23.20 PSV.dial :waste1 @15:32:23.92 PS.seek 0ml @15:32:50.44 PSV.dial PSR @15:32:50.96 PSR.select :air @15:32:50.99 PS.seek 2.5ml @15:33:17.29 PSV.dial PTV @15:33:18.01 PBV.dial PSV @15:33:18.20 PS.seek 0ml @15:33:44.70 PSV.dial PBV @15:33:45.16 PS.seek 2.5ml @15:34:11.54 PSV.dial :waste1 @15:34:12.39 PS.seek 0ml @15:34:38.79
Loading FlushPuck into Collection Clamp from Processing Clamp @15:34:38.98 Elbow.seek PC @15:34:41.50 Elbow.reconfigure :loose @15:34:41.60 Forearm.seek PC @15:34:42.72 Hand.close @15:34:43.43 PC.seek :open @15:34:47.94 Forearm.seek :retract @15:34:48.93 Elbow.reconfigure :tight @15:34:48.98 Elbow.seek CC @15:34:52.54 Elbow.reconfigure :loose @15:34:52.64 Forearm.reconfigure :tight @15:34:52.69 Forearm.seek CC @15:34:54.01 CC.seek :closed @15:34:57.98 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.97 @15:34:58.05 Hand.open @15:34:58.86 Forearm.reconfigure :loose @15:34:58.91 Forearm.seek :retract @15:34:59.90 Flushing the Collection Valve::Manifold @15:35:00.02 CSV.dial CSR @15:35:01.04 Pulling 5ml of CSR.flush CSR.select :flush @15:35:01.16 CS.seek 5ml @15:35:14.76 delay 5 seconds @15:35:19.78 CSR.select :air @15:35:19.95 CS.reconfigure :fast @15:35:20.00 CS.seek 7.5ml @15:35:26.54 CSV.dial :waste @15:35:27.81 CS.reconfigure :default @15:35:27.86 CS.seek 0ml @15:35:47.86 Flushing lower manifold's waste1 port @15:35:47.98 CSV.dial :flush @15:35:48.44 CS.reconfigure :fast @15:35:48.49 CS.seek 5ml @15:36:00.76 delay 5 seconds @15:36:05.78 CSV.dial :air @15:36:06.29 CS.seek 7.001ml @15:36:11.76 CSV.dial CTV @15:36:11.79
CTV.dial CSV @15:36:13.20 CBV.dial :waste1 @15:36:13.96 CS.seek 0ml @15:36:30.57 Flushing lower manifold's waste2 port @15:36:30.70 CSV.dial :flush @15:36:31.44 CS.seek 5ml @15:36:43.82 delay 5 seconds @15:36:48.84 CSV.dial :air @15:36:49.35 CS.seek 7ml @15:36:54.77 CSV.dial CTV @15:36:54.79
CTV.dial CSV @15:36:55.26 CBV.dial :waste2 @15:36:56.30 CS.seek 0ml @15:37:12.86 Flushing lower manifold's waste2b port @15:37:12.98 CSV.dial :flush @15:37:13.72 CS.seek 5ml @15:37:25.98 delay 5 seconds @15:37:31.00 CSV.dial :air @15:37:31.52 CS.seek 7ml @15:37:37.01 CSV.dial CTV @15:37:37.05
CTV.dial CSV @15:37:37.48 CBV.dial :waste2b @15:37:38.50 CS.seek 0ml @15:37:55.14 Disinfecting external Intake with kill @15:37:55.26 CSV.dial CSR @15:37:55.70 Pulling 8ml of CSR.kill @15:37:55.71 CSR.select :kill @15:37:55.82 CS.seek 8ml @15:38:14.97 delay 5 seconds @15:38:19.99 CSR.select :air @15:38:20.11 CS.seek 10ml @15:38:25.52 CSV.dial CBV @15:38:25.55
CBV.dial CSV @15:38:26.59 CTV.dial :intake @15:38:27.35 Intake.select :open @15:38:27.46 CS.seek 2ml @15:38:46.45 Intake.select :close @15:38:46.55 CTV.dial :air @15:38:47.33 CS.reconfigure :default @15:38:47.37 CS.seek 0ml @15:38:53.35 CSV.dial CSR @15:38:54.48 Pulling 5ml of CSR.flush @15:38:54.49 CSR.select :flush @15:38:54.60 CS.seek 5ml @15:39:08.29 delay 5 seconds @15:39:13.31 CSR.select :air @15:39:13.43 CS.seek 10ml @15:39:27.10 CSV.dial CBV @15:39:28.09 CS.seek 0ml @15:39:54.51 Garaging FlushPuck in Collection Clamp @15:39:54.58 Elbow.reconfigure :tight @15:39:54.63 Elbow.seek CC @15:39:54.80 Elbow.reconfigure :loose @15:39:54.95 Forearm.seek CC @15:39:55.92 Hand.close @15:39:56.68 CC.seek :open @15:40:00.78 Forearm.seek :retract @15:40:01.77 Elbow.reconfigure :tight @15:40:01.82 Elbow.seek :garage @15:40:04.82 Elbow.reconfigure :loose @15:40:04.93 Forearm.reconfigure :tight @15:40:04.97 Forearm.seek :garage @15:40:06.09 Hand.open @15:40:06.86 Forearm.reconfigure :loose @15:40:06.91 Forearm.seek :retract @15:40:07.91 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:25:28 @15:40:07.96 Asleep after completing DA_WCR_092325 assays @15:40:07.98 GoodNews.email "{1 => 12,\n 2 => 10,\n 3 => 22,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@15:34:58, 40% flow, 22.3C, 14% humidity, 14.7psia, 13.74V, 0.416A avg, 30.173Ah, 5.71W\nSampled 50.0ml",Subject: "Asleep after completing DA_WCR_092325 assays" @15:40:12.07 upsync + ESPwhich DA_WCR_093025.rb + fn=DA_WCR_093025.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_093025.rb + [ -f /home/esp/esp2/mission/DA_WCR_093025.rb ] + realpath /home/esp/esp2/mission/DA_WCR_093025.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_093025.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_093025.rb @14:24:09.98UTC30-Sep-25
ESP2 v3.42 I2C Gateway v3.20 for core bus @14:24:12.61 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @14:24:16.23 Polling Can every 10:00 @14:24:16.29 Cleanly exited at 14:21:10UTC30-Sep-25 @14:24:16.34 Loading /home/esp/esp2local/mission/DA_WCR_093025.rb mission @14:24:16.39 Preparing to sleep until + 00:00:05 @14:24:16.43 GoodNews.email "Starting DA_WCR_093025 w/pucks in tube 2\n{1 => 12,\n 2 => 10,\n 3 => 22,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@14:24:16, 39% flow, 21.3C, 17% humidity, 14.7psia, 13.76V, 0.303A avg, 64.806Ah, 4.16W",Subject: "DA_WCR_093025 begun" @14:24:16.47
Asleep til + 00:00:05 @14:24:16.49 Continuing at 14:24 @14:24:16.73 Gate.power :core,:OFF @14:24:16.74 Gate.power :raw,:OFF @14:24:16.75 Shutdown @14:24:18.94 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_093025.rb:14\n{1 => 12,\n 2 => 10,\n 3 => 22,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@14:24:16, 39% flow, 21.3C, 17% humidity, 14.7psia, 13.76V, 0.296A avg, 64.806Ah, 4.08W",Subject: "Asleep until 14:24" @14:24:20.34 upsync @14:24:21.38
Powering core bus @14:24:22.88 upsync @14:24:26.98
Gate.power :raw,:ON @14:24:27.69 CS.configure :default @14:24:27.73 PS.configure :default @14:24:27.78 SS.configure :default @14:24:27.83 CC.configure :default @14:24:27.88 PC.configure :default @14:24:27.95 Forearm.configure :loose @14:24:27.99 Elbow.configure :loose @14:24:28.06 SC.configure :loose @14:24:28.11 SE.configure :default @14:24:28.16 CH.configure :default @14:24:28.19 PH.configure :default @14:24:28.23 All dwarf controllers running firmware version 3.85 @14:24:28.34 Valve.reset @14:24:28.61 Intake.select :close @14:24:28.64 Exhaust.select :close @14:24:28.66 CTV.dial :air @14:24:28.75 PSV.dial :air @14:24:28.78 CSV.dial :waste1 @14:24:28.93
Forearm.home.seek :retract @14:24:29.30 SSV.dial :spit,avoiding: :exhaust @14:24:29.75 Hand.close @14:24:29.79
Elbow.reconfigure :loose @14:24:29.90 Elbow.jog 1500 @14:24:29.92 PS.home.seek 0ml @14:24:30.17 CS.reconfigure :fast @14:24:30.21 SS.home.seek 0ml @14:24:30.29 CS.home.seek 0ml @14:24:30.38
Elbow.reconfigure :loose @14:24:30.43 Elbow.home.seek :clear @14:24:33.59 SE.home.seek :down @14:24:34.21 SC.reconfigure :tight @14:24:34.26 SC.home.seek :initial @14:24:39.06 PS.stop @14:24:44.24
SC.configure :limp @14:24:44.30 Forearm.seek :retract @14:24:44.37 Elbow.seek :hammer @14:24:45.44 Forearm.reconfigure :tight @14:24:45.49 Forearm.seek retracted @14:24:45.61 Forearm.coast @14:24:45.65 Arm::StretchError in MAIN -- Holding position retracted requires 37mA @14:24:45.67 Retry #1 of 5 @14:24:45.74 Hammering Forearm against inboard stop to recenter bearing races @14:24:46.21 Forearm.reconfigure :loose @14:24:46.26 Forearm.seek between 4 and retracted @14:24:46.98 Forearm.force 100 @14:24:47.58 Forearm.stop @14:24:48.00 Forearm.reconfigure :tight @14:24:48.05 Forearm.seek retracted @14:24:48.39 PC.home.seek :open @14:24:48.46
CC.home.seek :open @14:24:51.55 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @14:24:51.56 Notice.email "At 14:24:51 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_093025.rb:14\n{1 => 12,\n 2 => 10,\n 3 => 22,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@14:24:51, 40% flow, 21.3C, 17% humidity, 14.7psia, 13.72V, 0.252A avg, 64.810Ah, 3.46W",Subject: "Started DA_WCR_093025 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @14:24:51.59
Starting initialPurge @14:24:51.60 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:24:51.68 Hand.open @14:24:52.60 Elbow.reconfigure :tight @14:24:52.66 Elbow.seek FlushPuck::Garage @14:24:54.52 Elbow.reconfigure :loose @14:24:54.62 Forearm.reconfigure :loose @14:24:54.67 Forearm.seek :garage @14:24:55.38 upsync @14:24:55.70
Hand.close @14:24:56.57 Forearm.seek :retract @14:24:57.69 Elbow.reconfigure :tight @14:24:57.74 Elbow.seek CC @14:25:00.54 Elbow.reconfigure :loose @14:25:00.65 Forearm.reconfigure :tight @14:25:00.70 Forearm.seek CC @14:25:01.93 CC.seek :closed @14:25:05.95 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.02 @14:25:06.01 Hand.open @14:25:06.77 Forearm.reconfigure :loose @14:25:06.82 Forearm.seek :retract @14:25:07.80 Flushing the Collection Valve::Manifold @14:25:07.92 CSV.dial CSR @14:25:09.29 Pulling 5ml of CSR.flush @14:25:09.30 CSR.select :flush @14:25:09.44 CS.reconfigure :default @14:25:09.49 CS.seek 5ml @14:25:23.42 delay 5 seconds @14:25:28.50 CSR.select :air @14:25:28.67 CS.reconfigure :fast @14:25:28.72 CS.seek 7.5ml @14:25:35.36 CSV.dial :waste @14:25:36.63 CS.reconfigure :default @14:25:36.67 CS.seek 0ml @14:25:56.67 Purging external Intake @14:25:56.79 CSV.dial :flush @14:25:57.25 CS.reconfigure :fast @14:25:57.30 CS.seek 10ml @14:26:21.17 delay 5 seconds @14:26:26.19 CSV.dial CBV @14:26:26.22
CBV.dial CSV @14:26:26.98 CTV.dial :intake @14:26:27.76 Intake.select :open @14:26:27.87 CS.seek 0ml @14:26:51.60 Intake.select :close @14:26:51.70 CTV.dial :air @14:26:52.48 Flushing upper manifold @14:26:52.60 CSV.dial :flush @14:26:53.33 CS.seek 5ml @14:27:05.67 delay 5 seconds @14:27:10.69 CSV.dial :air @14:27:11.18 CS.seek 7ml @14:27:16.61 CSV.dial CBV @14:27:16.63
CBV.dial CSV @14:27:17.65 CTV.dial :air @14:27:17.74 CS.seek 0ml @14:27:34.54 delay 7 seconds @14:27:41.57 CS.seek 3ml @14:27:49.39 CSV.dial :waste @14:27:49.84 CS.seek 0ml @14:27:57.65 CSV.dial :flush @14:27:58.10 CS.seek 3ml @14:28:05.85 delay 5 seconds @14:28:10.87 CSV.dial :air @14:28:11.37 CS.seek 5ml @14:28:16.88 CSV.dial :air @14:28:16.97 CS.seek 0ml @14:28:29.20 delay 7 seconds @14:28:36.23 CS.seek 3ml @14:28:44.04 CSV.dial :waste @14:28:44.77 CS.seek 0ml @14:28:52.45 Garaging FlushPuck in Collection Clamp @14:28:52.52 Elbow.reconfigure :tight @14:28:52.57 Elbow.seek CC @14:28:52.69 Elbow.reconfigure :loose @14:28:52.80 Forearm.seek CC @14:28:53.84 Hand.close @14:28:54.58 CC.seek :open @14:28:59.06 Forearm.seek :retract @14:29:00.06 Elbow.reconfigure :tight @14:29:00.11 Elbow.seek :garage @14:29:03.00 Elbow.reconfigure :loose @14:29:03.10 Forearm.reconfigure :tight @14:29:03.15 Forearm.seek :garage @14:29:04.27 Hand.open @14:29:05.01 Forearm.reconfigure :loose @14:29:05.05 Forearm.seek :retract @14:29:06.06 da sampling at most 1000ml, wcr at most 1000ml @14:29:06.10 Loading sh1 puck from tube 2 into Collection Clamp @14:29:06.11 DAfans.email "Started da in tube 2 at 14:29:06UTC30-Sep-25\n{1 => 12,\n 2 => 10,\n 3 => 22,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@14:24:51, 40% flow, 21.3C, 17% humidity, 14.7psia, 13.72V, 0.252A avg, 64.810Ah, 3.46W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @14:29:06.40
SC.reconfigure :tight @14:29:06.45 SC.seek 2 @14:29:09.83 upsync @14:29:13.28
SC.configure :limp @14:29:13.51 Elbow.reconfigure :tight @14:29:13.61 Elbow.seek 2 @14:29:15.59 Forearm.reconfigure :tight @14:29:15.64 Forearm.seek 2 + 800 counts @14:29:16.68 SE.reconfigure :pick @14:29:16.75 SE.seek :beamBreak @14:29:18.83 SE.configure :default @14:29:19.00 Elbow.seek 2 @14:29:19.18 Forearm.seek 2 + 300 counts @14:29:19.35 Forearm.reconfigure :loose @14:29:19.40 Hand.close @14:29:20.21 SE.seek :down @14:29:20.42
Forearm.seek :retract @14:29:21.81 Elbow.seek CC @14:29:22.19 SE.stop @14:29:23.35
Elbow.reconfigure :loose @14:29:23.45 Forearm.reconfigure :tight @14:29:23.50 Forearm.seek CC @14:29:24.77 CC.seek :closed @14:29:28.79 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.10 @14:29:28.80 Hand.open @14:29:29.64 Forearm.reconfigure :loose @14:29:29.68 Forearm.seek :retract @14:29:30.68 collectSample 1000ml w/bubble point of 24psi @14:29:30.69 Priming 7ml @14:29:30.71 Intake.select :close @14:29:30.72 Exhaust.select :close @14:29:30.73 SS.hold @14:29:30.93 Calibrating pressure and temperature sensors @14:29:30.96 CTV.dial :air @14:29:30.98 SSV.dial :spit,avoiding: :exhaust @14:29:31.05 SS.reconfigure :fast @14:29:31.10 SS.seek 5ml @14:29:34.19 SSV.dial CBV @14:29:34.23
CBV.dial SSV @14:29:35.57 delay 3.8 seconds @14:29:39.49 Sensor limits: Intake max 107.0psia; Outlet max 113.6psia @14:29:39.50 SSV.dial :spit @14:29:40.84 SS.seek 0ml @14:29:43.36 SSV.dial CBV @14:29:44.70 CTV.dial :intake @14:29:45.48 SSV.dial CBV @14:29:45.51
CBV.dial SSV @14:29:45.64 Allowing 40s for 7.0ml of remaining 7.0ml @14:29:45.65 Intake.select :open @14:29:47.81 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:29:47.83 SS.reconfigure :inhaling 2.17ml/s @14:29:47.88 SS.seek 7ml @14:29:54.64 Stroke completed -- waiting for pressure to equalize ... @14:29:54.65 SS.reconfigure :exhale @14:29:54.69 SS.hold @14:29:56.73 Intake.select :close @14:29:56.88 SSV.dial :exhaust @14:29:57.66 Exhaust.select :open @14:29:57.77 SS.seek 0ml @14:30:01.87 Exhaust.select :close @14:30:01.99 Sampling 1000.0ml @14:30:02.00 Intake.select :close @14:30:02.01 Exhaust.select :close @14:30:02.02 SS.reconfigure :default @14:30:02.07 SS.hold @14:30:02.27 CTV.dial :intake @14:30:02.45 SSV.dial CBV @14:30:02.48
CBV.dial SSV @14:30:03.27 Allowing 130s for 25.0ml of remaining 1000.0ml @14:30:03.28 Intake.select :open @14:30:05.44 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:30:05.46 SS.reconfigure :inhaling 2.17ml/s @14:30:05.51 SS.seek 25ml @14:30:10.29 Vacuum too high -- waiting up to 40 seconds @14:30:10.30 SS.reconfigure :exhale @14:30:10.36 SS.hold @14:30:51.00 SS.reconfigure :default @14:30:51.05 SS.hold @14:30:51.12 Intake.select :close @14:30:51.27 ShallowSampler::Clogged in MAIN -- Intake @14.4psia, & Outlet @2.4psia -- no progress after 5.4ml @14:30:51.29 SSV.dial :exhaust @14:30:52.05 Exhaust.select :open @14:30:52.16 SS.reconfigure :exhale @14:30:52.21 SS.seek 0ml @14:30:56.07 Exhaust.select :close @14:30:56.18 Sampled 5.4ml @14:30:56.20 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @14:30:56.27 CSV.dial :waste @14:30:56.44 SSV.dial CBV,avoiding: :exhaust @14:30:56.46 CS.seek 0ml @14:30:56.49
CBV.dial SSV @14:30:56.64 CSV.dial :air @14:30:57.36 CS.seek 10ml @14:30:58.41
CTV.dial :air @14:30:59.17 delay 15 seconds @14:31:14.19 SSV.dial :spit,avoiding: :exhaust @14:31:15.52 CTV.dial CSV,avoiding: :intake @14:31:15.55
SS.seek 0ml @14:31:15.68 SSV.dial CBV,avoiding: :exhaust @14:31:21.29 SSV.dial between CBV and plug @14:31:21.78 CBV.dial between SSV and waste2 @14:31:22.18 CTV.dial CSV,avoiding: :air @14:31:22.22
CSV.dial CTV @14:31:22.70 CS.reconfigure :pressurize @14:31:22.75 CS.seek 0ml @14:31:47.11 BubblePoint: Intake @24.0psia, Outlet @-7.9psia @14:31:47.12 CBV.dial SSV @14:31:47.46 SS.reconfigure :default @14:31:47.51 SS.seek 10ml @14:31:53.20 delay 15 seconds @14:32:08.33 Evacuated: Intake @22.4psia, Outlet @16.8psia @14:32:08.34 SSV.dial :spit,avoiding: :exhaust @14:32:09.48 SS.reconfigure :exhale @14:32:09.53 SS.seek 0ml @14:32:14.59 SS.reconfigure :fast @14:32:14.63 SS.seek 5ml @14:32:17.69 SSV.dial CBV,avoiding: :exhaust @14:32:17.72
CBV.dial SSV @14:32:19.06 CTV.dial :air,avoiding: :intake @14:32:20.45 CSV.dial CTV @14:32:20.49
SS.seek 10ml @14:32:21.61 CSV.dial :air @14:32:22.07 CS.reconfigure :fast @14:32:22.12 CS.seek 0ml @14:32:23.16
SSV.dial :spit,avoiding: :exhaust @14:32:24.52 CS.stop @14:32:24.56
SS.reconfigure :exhale @14:32:24.63 SS.seek 0ml @14:32:29.76 SS.reconfigure :fast @14:32:29.81 SS.seek 5ml @14:32:32.66 SSV.dial CBV,avoiding: :exhaust @14:32:32.68
CBV.dial SSV @14:32:35.07 SSV.dial :spit,avoiding: :exhaust @14:32:36.41 SS.reconfigure :default @14:32:36.46 SS.seek 0ml @14:32:39.79 SS.stop @14:32:39.84 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:32:39.85 Intake.select :close @14:32:39.91 CSV.dial :waste @14:32:40.05 SSV.dial CBV,avoiding: :exhaust @14:32:40.07
CBV.dial SSV @14:32:40.65 CS.seek 0ml @14:32:40.72 CSV.dial :air @14:32:41.46
CTV.dial :air @14:32:41.50 CS.seek 10ml @14:32:41.62
delay 15 seconds @14:32:56.64 SSV.dial :spit,avoiding: :exhaust @14:32:57.99 CTV.dial CSV,avoiding: :intake @14:32:58.02
SS.reconfigure :exhale @14:32:58.12 SS.seek 0ml @14:32:58.22 SSV.dial CBV,avoiding: :exhaust @14:33:05.48 SSV.dial between CBV and plug @14:33:05.97 CBV.dial between SSV and waste2 @14:33:06.38 CSV.dial between air and CTV @14:33:06.69 CS.seek 8.379ml @14:33:11.26 CTV.dial CSV,avoiding: :air @14:33:11.29
CSV.dial CTV @14:33:11.59 CS.reconfigure :pressurize @14:33:11.64 CS.seek 0ml @14:33:31.55 BubblePoint: Intake @24.0psia, Outlet @-0.4psia @14:33:31.56 CBV.dial SSV @14:33:31.90 SS.reconfigure :default @14:33:31.95 SS.seek 10ml @14:33:37.56 delay 15 seconds @14:33:52.76 Evacuated: Intake @22.6psia, Outlet @15.6psia @14:33:52.77 SSV.dial :spit,avoiding: :exhaust @14:33:53.81 SS.reconfigure :exhale @14:33:53.86 SS.seek 0ml @14:33:59.01 SS.reconfigure :fast @14:33:59.05 SS.seek 5ml @14:34:01.86 SSV.dial CBV,avoiding: :exhaust @14:34:01.89
CBV.dial SSV @14:34:03.26 CTV.dial :air,avoiding: :intake @14:34:04.62 CSV.dial CTV @14:34:04.66
SS.seek 10ml @14:34:05.82 CSV.dial :air @14:34:06.26 CS.reconfigure :fast @14:34:06.31 CS.seek 0ml @14:34:07.37
SSV.dial :spit,avoiding: :exhaust @14:34:08.72 SS.reconfigure :exhale @14:34:08.77 SS.seek 0ml @14:34:08.91 CS.stop @14:34:13.77
SS.reconfigure :fast @14:34:13.82 SS.seek 5ml @14:34:16.69 SSV.dial CBV,avoiding: :exhaust @14:34:16.71
CBV.dial SSV @14:34:19.07 SSV.dial :spit,avoiding: :exhaust @14:34:20.39 SS.reconfigure :default @14:34:20.44 SS.seek 0ml @14:34:23.65 SS.stop @14:34:23.69 Lysing: Adding 2.0ml AqMeOH @14:34:23.70 CBV.dial between CSV and waste1,avoiding: :DBV @14:34:25.21 CTV.dial CSV @14:34:26.55 CSV.dial CSR @14:34:27.34 Pulling 0.999ml of CSR.AqMeOH @14:34:27.35 CSR.select :AqMeOH @14:34:27.47 CS.reconfigure :default @14:34:27.51 CS.seek 1ml @14:34:30.88 delay 5 seconds @14:34:35.90 CSR.select :air @14:34:36.07 CS.seek 6ml @14:34:49.82 delay 2 seconds @14:34:51.83 CSV.dial :waste2 @14:34:52.28 CS.reconfigure :fast @14:34:52.32 CS.seek 0ml @14:35:06.72 CSV.dial CSR @14:35:07.18 Pulling 2ml of CSR.AqMeOH @14:35:07.19 CSR.select :AqMeOH @14:35:07.30 CS.reconfigure :default @14:35:07.35 CS.seek 2ml @14:35:13.35 delay 5 seconds @14:35:18.37 CSR.select :air @14:35:18.53 CS.seek 5ml @14:35:27.07 delay 2 seconds @14:35:29.08 CSV.dial CTV @14:35:29.53 CS.seek 1ml @14:35:40.89 Before lysing Collection Puck Heater at 19.3C/14.6psia @14:35:40.91 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @14:35:40.93 CH.seek 70C,8:00 @14:37:56.47 CH.hold 10:00,70C @14:37:56.67 Begun lysing Collection Puck Heater at 68.1C/20.8psia @14:47:56.68 Ended lysing Collection Puck Heater at 70C/19.7psia @14:47:56.69 CS.seek 0ml @14:48:00.06 CTV.dial between spare and air,avoiding: :intake @14:48:01.12 CSV.dial :air @14:48:01.56 CS.seek 5ml @14:48:15.21 CSV.dial CTV @14:48:15.67 delay 2 seconds @14:48:17.68 CSV.dial :air @14:48:18.13 delay 2 seconds @14:48:20.15 CSV.dial CTV @14:48:20.60 delay 2 seconds @14:48:22.62 CSV.dial :air @14:48:23.07 delay 2 seconds @14:48:25.09 CS.seek 0ml @14:48:38.79 Puck @26.8psia after final lysis pressurization @14:48:38.80 Recovering Lysate into Collection syringe @14:48:38.81 CSV.dial CBV @14:48:39.79 CBV.dial CSV @14:48:40.16 CS.seek 5ml @14:48:53.77 delay 5 seconds @14:48:58.79 CTV.dial :air @14:48:59.32 CS.seek 7ml @14:49:05.37 delay 5 seconds @14:49:10.39 CSV.dial :air2 @14:49:10.83 CS.seek 10ml @14:49:19.34 CSV.dial between air and CTV,avoiding: CBV @14:49:20.59 Lysing Complete @14:49:20.66 Storing sh1 puck in Collection Clamp to tube 1 @14:49:20.83 SC.reconfigure :tight @14:49:20.88 SC.seek 1 @14:49:24.47 SC.configure :limp @14:49:24.68 Elbow.reconfigure :tight @14:49:24.73 Elbow.seek 1 @14:49:26.44 Forearm.reconfigure :tight @14:49:26.48 Forearm.seek 1 + 800 counts @14:49:27.97 SE.reconfigure :pick @14:49:28.03 SE.seek :beamBreak @14:49:29.95 SE.configure :default @14:49:30.12 Elbow.reconfigure :limp @14:49:30.17 Forearm.reconfigure :loose @14:49:30.21 Forearm.seek 1 @14:49:30.57 Elbow.hold @14:49:30.63 Elbow.reconfigure :tight @14:49:30.73 SE.seek 11.1 Pucks below Top Plate @14:49:31.27 Forearm.force -20 @14:49:31.31 Forearm.seek 1 + 289 counts @14:49:31.58 Forearm.reconfigure :tight @14:49:31.62 Forearm.seek 1 + 800 counts @14:49:31.99 SE.seek 12.1 Pucks below Top Plate @14:49:32.63 Forearm.reconfigure :loose @14:49:32.68 Forearm.seek :retract @14:49:33.60 Elbow.seek CC @14:49:35.22 Elbow.reconfigure :loose @14:49:35.33 Forearm.seek CC @14:49:36.29 Hand.close @14:49:37.05 CC.seek :open @14:49:41.25 Forearm.seek :retract @14:49:42.29 Elbow.reconfigure :tight @14:49:42.34 Elbow.seek 1 @14:49:44.07 Elbow.reconfigure :limp @14:49:44.12 Forearm.seek 1 @14:49:45.25 Elbow.hold @14:49:45.32 Elbow.reconfigure :tight @14:49:45.37 Hand.open @14:49:46.17 Forearm.force -20 @14:49:46.21 Forearm.seek 1 + 319 counts @14:49:46.97 Forearm.reconfigure :tight @14:49:47.08 Forearm.seek 1 + 800 counts @14:49:47.37 SE.seek :down @14:49:48.51
Forearm.reconfigure :loose @14:49:48.56 Forearm.seek :retract @14:49:48.93 SE.stop @14:49:49.54
Tranferring DA @14:49:49.55 Beginning samplePassC @14:49:49.56 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:49:49.75 Elbow.seek FlushPuck::Garage @14:49:51.54 Elbow.reconfigure :loose @14:49:51.64 Forearm.seek :garage @14:49:52.71 Hand.close @14:49:53.55 Forearm.seek :retract @14:49:54.55 Elbow.reconfigure :tight @14:49:54.60 Elbow.seek CC @14:49:57.42 Elbow.reconfigure :loose @14:49:57.52 Forearm.reconfigure :tight @14:49:57.56 Forearm.seek CC @14:49:58.91 CC.seek :closed @14:50:02.93 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.10 @14:50:03.00 Hand.open @14:50:03.74 Forearm.reconfigure :loose @14:50:03.78 Forearm.seek :retract @14:50:04.85 PSV.dial PSR @14:50:06.22 Pulling 1.5ml of PSR.flush @14:50:06.23 PSR.select :flush @14:50:06.35 PS.seek 1.5ml @14:50:22.55 delay 5 seconds @14:50:27.57 PSV.dial :waste1 @14:50:28.01 PS.seek 0ml @14:50:44.16 PSV.dial PSR @14:50:44.62 PSR.select :air @14:50:44.73 PS.seek 2.5ml @14:51:11.14 PRV.dial CBV,avoiding: :ab2 @14:51:11.16
CBV.dial PRV @14:51:12.93 PSV.dial :mixloop @14:51:13.96 CTV.dial :air,avoiding: :intake @14:51:14.04 PTV.dial :PRVmixing @14:51:15.34 PS.seek 0ml @14:51:41.66 PSV.dial :air @14:51:42.12 PS.seek 2.5ml @14:52:08.53 PSV.dial :mixloop @14:52:09.00 PS.seek 0ml @14:52:35.38 PSV.dial :air @14:52:35.84 PS.seek 2.5ml @14:53:02.19 PSV.dial :mixloop @14:53:02.65 PS.seek 0ml @14:53:29.04 Garaging FlushPuck in Collection Clamp @14:53:29.11 Elbow.reconfigure :tight @14:53:29.15 Elbow.seek CC @14:53:29.27 Elbow.reconfigure :loose @14:53:29.37 Forearm.seek CC @14:53:30.44 Hand.close @14:53:31.17 CC.seek :open @14:53:35.29 Forearm.seek :retract @14:53:36.27 Elbow.reconfigure :tight @14:53:36.31 Elbow.seek :garage @14:53:39.21 Elbow.reconfigure :loose @14:53:39.31 Forearm.reconfigure :tight @14:53:39.36 Forearm.seek :garage @14:53:40.49 Hand.open @14:53:41.23 Forearm.reconfigure :loose @14:53:41.28 Forearm.seek :retract @14:53:42.36 PSV.dial :mixloop @14:53:42.43 PTV.dial :PRVmixing @14:53:42.94 PRV.dial CBV @14:53:42.97
CBV.dial PRV @14:53:43.19 CTV.dial :air,avoiding: :intake @14:53:43.27 Loading lyfil puck from tube 2 into Collection Clamp @14:53:43.52 SC.reconfigure :tight @14:53:43.57 SC.seek 2 @14:53:47.19 SC.configure :limp @14:53:47.41 Elbow.reconfigure :tight @14:53:47.46 Elbow.seek 2 @14:53:49.38 Forearm.reconfigure :tight @14:53:49.43 Forearm.seek 2 + 800 counts @14:53:50.50 SE.reconfigure :pick @14:53:50.56 SE.seek :beamBreak @14:53:52.88 SE.configure :default @14:53:53.04 Elbow.seek 2 @14:53:53.27 Forearm.seek 2 + 300 counts @14:53:53.44 Forearm.reconfigure :loose @14:53:53.49 Hand.close @14:53:54.19 SE.seek :down @14:53:54.39
Forearm.seek :retract @14:53:55.50 Elbow.seek CC @14:53:56.21 SE.stop @14:53:57.07
Elbow.reconfigure :loose @14:53:57.17 Forearm.reconfigure :tight @14:53:57.22 Forearm.seek CC @14:53:58.85 CC.seek :closed @14:54:02.84 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 2.01 @14:54:02.86 Hand.open @14:54:03.61 Forearm.reconfigure :loose @14:54:03.65 Forearm.seek :retract @14:54:04.61 CTV.dial CSV,avoiding: :intake @14:54:05.97 CSV.dial CTV @14:54:06.28 PS.seek 2.5ml @14:54:32.69 CS.seek 5ml @14:54:46.33 delay 8 seconds @14:54:54.35 PSV.dial :air @14:54:54.80 delay 2 seconds @14:54:56.82 PSV.dial :waste2 @14:54:57.56 PS.seek 0ml @14:55:23.91 CBV.dial between PRV and waste1 @14:55:24.24 CSV.dial :air @14:55:24.70 CS.seek 10ml @14:55:38.36 CSV.dial CTV @14:55:38.82 CS.seek 0ml @14:56:05.17 CBV.dial :waste1 @14:56:05.49 delay 5 seconds @14:56:10.51 CTV.dial :air,avoiding: :intake @14:56:11.93 Storing lyfil puck in Collection Clamp to tube 1 @14:56:12.11 SC.reconfigure :tight @14:56:12.16 SC.seek 1 @14:56:15.76 SC.configure :limp @14:56:15.98 Elbow.reconfigure :tight @14:56:16.02 Elbow.seek 1 @14:56:17.74 Forearm.reconfigure :tight @14:56:17.79 Forearm.seek 1 + 800 counts @14:56:18.92 SE.reconfigure :pick @14:56:18.98 SE.seek :beamBreak @14:56:20.95 SE.configure :default @14:56:21.11 Elbow.reconfigure :limp @14:56:21.16 Forearm.reconfigure :loose @14:56:21.21 Forearm.seek 1 @14:56:21.56 Elbow.hold @14:56:21.63 Elbow.reconfigure :tight @14:56:21.73 SE.seek 12.1 Pucks below Top Plate @14:56:22.21 Forearm.force -20 @14:56:22.25 Forearm.seek 1 + 318 counts @14:56:22.54 Forearm.reconfigure :tight @14:56:22.59 Forearm.seek 1 + 800 counts @14:56:22.83 SE.seek 13.1 Pucks below Top Plate @14:56:23.41 Forearm.reconfigure :loose @14:56:23.45 Forearm.seek :retract @14:56:24.34 Elbow.seek CC @14:56:25.95 Elbow.reconfigure :loose @14:56:26.05 Forearm.seek CC @14:56:27.01 Hand.close @14:56:27.80 CC.seek :open @14:56:32.00 Forearm.seek :retract @14:56:33.05 Elbow.reconfigure :tight @14:56:33.09 Elbow.seek 1 @14:56:34.78 Elbow.reconfigure :limp @14:56:34.84 Forearm.seek 1 @14:56:35.96 Elbow.hold @14:56:36.46 Elbow.reconfigure :tight @14:56:36.51 Hand.open @14:56:37.41 Forearm.force -20 @14:56:37.46 Forearm.seek 1 + 317 counts @14:56:37.85 Forearm.reconfigure :tight @14:56:37.90 Forearm.seek 1 + 800 counts @14:56:38.18 SE.seek :down @14:56:39.32
Forearm.reconfigure :loose @14:56:39.37 Forearm.seek :retract @14:56:39.63 SE.stop @14:56:40.40
PSV.dial :air @14:56:41.15 PS.seek 2.5ml @14:57:07.50 delay 1 second @14:57:08.52 PSV.dial :mixloop @14:57:09.08 delay 1 second @14:57:10.10 PSV.dial :air @14:57:10.53 delay 1 second @14:57:11.55 PSV.dial :mixloop @14:57:12.00 delay 1 second @14:57:13.02 PSV.dial :air @14:57:13.47 delay 1 second @14:57:14.49 PS.seek 0ml @14:57:41.07 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:57:41.25 Elbow.seek FlushPuck::Garage @14:57:43.05 Elbow.reconfigure :loose @14:57:43.15 Forearm.seek :garage @14:57:44.18 Hand.close @14:57:45.03 Forearm.seek :retract @14:57:46.04 Elbow.reconfigure :tight @14:57:46.10 Elbow.seek PC @14:57:47.43 Elbow.reconfigure :loose @14:57:47.53 Forearm.reconfigure :tight @14:57:47.57 Forearm.seek PC @14:57:48.98 PC.seek :closed @14:57:53.41 Processing Clamp onSpring/offSpring for 12/26 samples, on/off torque 1.55 @14:57:53.47 Hand.open @14:57:54.23 Forearm.reconfigure :loose @14:57:54.27 Forearm.seek :retract @14:57:55.35 PRV.dial :air @14:57:55.80 PSV.dial :mixloop @14:57:56.32 PS.seek 0.3ml @14:58:00.25 PSV.dial PSR @14:58:01.41 Pulling 0.4ml of PSR.air @14:58:01.42 PSR.select :air @14:58:01.45 PS.seek 0.7ml @14:58:06.34 delay 3 seconds @14:58:09.36 PSR.select :air @14:58:09.39 PS.seek 2.5ml @14:58:28.53 delay 3 seconds @14:58:31.55 PSV.dial :mixloop @14:58:32.56 PTV.dial :mixing @14:58:33.30 PBV.dial :waste2 @14:58:34.29 PS.seek 0ml @14:59:00.63 Garaging FlushPuck in Processing Clamp @14:59:00.70 Elbow.reconfigure :tight @14:59:00.74 Elbow.seek PC @14:59:00.91 Elbow.reconfigure :loose @14:59:01.01 Forearm.seek PC @14:59:02.12 Hand.close @14:59:02.92 PC.seek :open @14:59:07.43 Forearm.seek :retract @14:59:08.56 Elbow.reconfigure :tight @14:59:08.61 Elbow.seek :garage @14:59:10.48 Elbow.reconfigure :loose @14:59:10.58 Forearm.reconfigure :tight @14:59:10.63 Forearm.seek :garage @14:59:11.76 Hand.open @14:59:12.62 Forearm.reconfigure :loose @14:59:12.66 Forearm.seek :retract @14:59:13.65 Loading da puck from tube 2 into Processing Clamp @14:59:13.89 SC.reconfigure :tight @14:59:13.94 SC.seek 2 @14:59:17.59 SC.configure :limp @14:59:17.80 Elbow.reconfigure :tight @14:59:17.85 Elbow.seek 2 @14:59:19.75 Forearm.reconfigure :tight @14:59:19.80 Forearm.seek 2 + 800 counts @14:59:20.98 SE.reconfigure :pick @14:59:21.03 SE.seek :beamBreak @14:59:23.32 SE.configure :default @14:59:23.48 Elbow.seek 2 @14:59:23.66 Forearm.seek 2 + 300 counts @14:59:23.90 Forearm.reconfigure :loose @14:59:23.95 Hand.close @14:59:24.68 SE.seek :down @14:59:24.88
Forearm.seek :retract @14:59:25.82 Elbow.seek PC @14:59:26.80 SE.stop @14:59:28.37
Elbow.reconfigure :loose @14:59:28.47 Forearm.reconfigure :tight @14:59:28.51 Forearm.seek PC @14:59:30.34 PC.seek :closed @14:59:34.75 Processing Clamp onSpring/offSpring for 12/26 samples, on/off torque 1.57 @14:59:34.76 Hand.open @14:59:35.60 Forearm.reconfigure :loose @14:59:35.65 Forearm.seek :retract @14:59:36.72 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @14:59:36.74 PH.seek 28C,5:00 @15:00:20.50 PH.hold 2:00:00,28C @15:00:20.55 delay 1:30 @15:01:50.59 PSR.select :air @15:01:50.61 PSV.dial PSR @15:01:51.64 Pulling 0.5ml of PSR.wash @15:01:51.65 PSR.select :wash @15:01:51.77 PS.seek 0.5ml @15:01:57.65 delay 5 seconds @15:02:02.67 PSR.select :air @15:02:02.80 PS.seek 2ml @15:02:19.00 PTV.dial :puck @15:02:19.73 PSV.dial PTV @15:02:20.48 PBV.dial :waste1 @15:02:21.90 PS.seek 1.5ml @15:02:27.84 delay 3 seconds @15:02:30.86 PBV.dial between PSV and waste1 @15:02:31.24 PS.seek 0ml @15:02:47.53 delay 5:00 @15:07:47.55 recoverPSRreagent08 @15:07:47.56 PTV.dial :puck @15:07:47.63 PBV.dial :waste1 @15:07:48.03 delay 30 seconds @15:08:18.05 PBV.dial between PSV and waste1 @15:08:18.43 PSR.select :air @15:08:18.45 PSV.dial PSR @15:08:19.31 PS.reconfigure :fast @15:08:19.36 PS.seek 2.5ml @15:08:42.87 PSV.dial PTV @15:08:43.60 delay 5 seconds @15:08:48.62 PSV.dial PSR @15:08:49.49 delay 5 seconds @15:08:54.51 PSV.dial PTV @15:08:55.24 delay 5 seconds @15:09:00.26 PSV.dial :air @15:09:01.01 PS.seek 0ml @15:09:24.37 PTV.dial :PRVmixing,via: PRV @15:09:25.66 PSV.dial :mixloop @15:09:26.12 PRV.dial :ab1 @15:09:26.86 PS.reconfigure :slow @15:09:26.90 PS.seek 0.15ml @15:09:30.52 delay 2 seconds @15:09:32.54 CBV.dial PRV @15:09:32.58
PRV.dial CBV @15:09:33.12 PS.seek 0.34ml @15:09:37.49 delay 2 seconds @15:09:39.51 CBV.dial between PRV and PSV @15:09:39.83 PRV.dial :air @15:09:40.30 PS.seek 0.9ml @15:09:52.27 PRV.dial :waste2 @15:09:53.42 PS.seek 0.5ml @15:10:02.02 addPMLReagent08 @15:10:02.03 PSV.dial :air @15:10:02.48 PS.reconfigure :default @15:10:02.53 PS.seek 2ml @15:10:18.65 PSV.dial :PML @15:10:19.10 PTV.dial :mixing @15:10:19.84 PBV.dial :waste1 @15:10:20.27 PS.seek 1.85ml @15:10:22.58 PBV.dial between PSV and waste1 @15:10:22.97 PS.seek 0ml @15:10:42.65 delay 2 seconds @15:10:44.67 Beginning samplePassC @15:10:44.68 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:10:44.87 Elbow.reconfigure :tight @15:10:44.91 Elbow.seek FlushPuck::Garage @15:10:46.29 Elbow.reconfigure :loose @15:10:46.39 Forearm.seek :garage @15:10:47.47 Hand.close @15:10:48.28 Forearm.seek :retract @15:10:49.31 Elbow.reconfigure :tight @15:10:49.36 Elbow.seek CC @15:10:52.19 Elbow.reconfigure :loose @15:10:52.29 Forearm.reconfigure :tight @15:10:52.34 Forearm.seek CC @15:10:53.71 CC.seek :closed @15:10:57.71 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.93 @15:10:57.78 Hand.open @15:10:58.97 Forearm.reconfigure :loose @15:10:59.02 Forearm.seek :retract @15:11:00.07 PSV.dial PSR @15:11:01.10 Pulling 1.5ml of PSR.flush @15:11:01.11 PSR.select :flush @15:11:01.23 PS.seek 1.5ml @15:11:17.33 delay 5 seconds @15:11:22.35 PSV.dial :waste1 @15:11:22.82 PS.seek 0ml @15:11:38.93 PSV.dial PSR @15:11:39.40 PSR.select :air @15:11:39.51 PS.seek 2.5ml @15:12:05.87 PRV.dial CBV,avoiding: :ab2 @15:12:05.89
CBV.dial PRV @15:12:07.54 PSV.dial :mixloop @15:12:08.55 CTV.dial :air,avoiding: :intake @15:12:08.63 PTV.dial :PRVmixing @15:12:09.39 PS.seek 0ml @15:12:35.68 PSV.dial :air @15:12:36.14 PS.seek 2.5ml @15:13:02.52 PSV.dial :mixloop @15:13:02.98 PS.seek 0ml @15:13:29.49 PSV.dial :air @15:13:29.95 PS.seek 2.5ml @15:13:56.27 PSV.dial :mixloop @15:13:56.73 PS.seek 0ml @15:14:23.18 Garaging FlushPuck in Collection Clamp @15:14:23.25 Elbow.reconfigure :tight @15:14:23.30 Elbow.seek CC @15:14:23.61 Elbow.reconfigure :loose @15:14:23.71 Forearm.seek CC @15:14:24.75 Hand.close @15:14:25.53 CC.seek :open @15:14:29.65 Forearm.seek :retract @15:14:30.70 Elbow.reconfigure :tight @15:14:30.75 Elbow.seek :garage @15:14:33.53 Elbow.reconfigure :loose @15:14:34.07 Forearm.reconfigure :tight @15:14:34.12 Forearm.seek :garage @15:14:35.26 Hand.open @15:14:36.09 Forearm.reconfigure :loose @15:14:36.14 Forearm.seek :retract @15:14:37.14 delayUntil "15:18:44.65" @15:14:37.16 Whole Cell Archive sampling 1000ml @15:14:37.18 Loading wcr puck from tube 2 into Collection Clamp @15:14:37.67 Elbow.reconfigure :tight @15:14:37.72 Elbow.seek 2 @15:14:39.63 Forearm.reconfigure :tight @15:14:39.68 Forearm.seek 2 + 800 counts @15:14:40.74 SE.reconfigure :pick @15:14:40.81 SE.seek :beamBreak @15:14:43.21 SE.configure :default @15:14:43.38 Elbow.seek 2 @15:14:43.57 Forearm.seek 2 + 300 counts @15:14:43.87 Forearm.reconfigure :loose @15:14:43.92 Hand.close @15:14:44.66 SE.seek :down @15:14:44.88 Forearm.seek :retract @15:14:45.84 Elbow.seek CC @15:14:47.02 SE.stop @15:14:47.39 Elbow.reconfigure :loose @15:14:47.50 Forearm.reconfigure :tight @15:14:47.55 Forearm.seek CC @15:14:49.22 CC.seek :closed @15:14:53.25 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.00 @15:14:53.26 Hand.open @15:14:54.10 Forearm.reconfigure :loose @15:14:54.15 Forearm.seek :retract @15:14:55.11 collectSample 1000ml w/bubble point of 35psi @15:14:55.12 Priming 7ml @15:14:55.13 Intake.select :close @15:14:55.14 Exhaust.select :close @15:14:55.15 SS.hold @15:14:55.37 Calibrating pressure sensors @15:14:55.38 CTV.dial :air @15:14:55.39 SSV.dial :spit,avoiding: :exhaust @15:14:55.41 SS.reconfigure :fast @15:14:55.46 SS.seek 5ml @15:14:58.40 SSV.dial CBV @15:14:58.43 CBV.dial SSV @15:14:59.82 delay 3.8 seconds @15:15:03.75 Sensor limits: Intake max 111.3psia; Outlet max 113.4psia @15:15:03.76 SSV.dial :spit @15:15:05.09 SS.seek 0ml @15:15:07.62 SSV.dial CBV @15:15:08.96 CTV.dial :intake @15:15:09.74 SSV.dial CBV @15:15:09.77 CBV.dial SSV @15:15:09.90 Allowing 40s for 7.0ml of remaining 7.0ml @15:15:09.92 Intake.select :open @15:15:12.08 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:15:12.10 SS.reconfigure :inhaling 2.17ml/s @15:15:12.15 SS.seek 7ml @15:15:18.73 Stroke completed -- waiting for pressure to equalize ... @15:15:18.74 SS.reconfigure :exhale @15:15:18.78 SS.hold @15:15:19.57 Intake.select :close @15:15:19.74 SSV.dial :exhaust @15:15:20.51 Exhaust.select :open @15:15:20.62 SS.seek 0ml @15:15:24.45 Exhaust.select :close @15:15:24.56 Sampling 1000.0ml @15:15:24.57 Intake.select :close @15:15:24.58 Exhaust.select :close @15:15:24.60 SS.reconfigure :default @15:15:24.64 SS.hold @15:15:24.85 CTV.dial :intake @15:15:24.87 SSV.dial CBV @15:15:24.89 CBV.dial SSV @15:15:25.68 Allowing 130s for 25.0ml of remaining 1000.0ml @15:15:25.69 Intake.select :open @15:15:27.86 Intake @14.9psia, vacuum from 9.4 to 12.4psi @15:15:27.88 SS.reconfigure :inhaling 2.17ml/s @15:15:27.93 SS.seek 25ml @15:15:42.79 Stroke completed -- waiting for pressure to equalize ... @15:15:42.80 SS.reconfigure :exhale @15:15:42.85 SS.hold @15:15:43.21 Intake.select :close @15:15:43.37 SSV.dial :exhaust @15:15:44.13 Exhaust.select :open @15:15:44.25 SS.seek 0ml @15:15:53.60 Exhaust.select :close @15:15:53.71 SSV.dial CBV @15:15:53.75 CBV.dial SSV @15:15:54.49 Allowing 130s for 25.0ml of remaining 975.0ml @15:15:54.51 Intake.select :open @15:15:56.68 Intake @14.9psia, vacuum from 9.4 to 12.4psi @15:15:56.69 SS.reconfigure :inhaling 2.17ml/s @15:15:56.74 SS.seek 25ml @15:16:10.90 Stroke completed -- waiting for pressure to equalize ... @15:16:10.91 SS.reconfigure :exhale @15:16:10.96 SS.hold @15:16:13.91 Intake.select :close @15:16:14.08 SSV.dial :exhaust @15:16:14.84 Exhaust.select :open @15:16:14.95 SS.seek 0ml @15:16:23.65 Exhaust.select :close @15:16:23.76 SSV.dial CBV @15:16:23.79 CBV.dial SSV @15:16:24.58 Allowing 130s for 25.0ml of remaining 950.0ml @15:16:24.60 Intake.select :open @15:16:26.77 Intake @14.9psia, vacuum from 9.4 to 12.4psi @15:16:26.79 SS.reconfigure :inhaling 2.17ml/s @15:16:26.83 SS.seek 25ml @15:16:41.75 Stroke completed -- waiting for pressure to equalize ... @15:16:41.77 SS.reconfigure :exhale @15:16:41.82 SS.hold @15:16:53.40 Intake.select :close @15:16:53.57 SSV.dial :exhaust @15:16:54.33 Exhaust.select :open @15:16:54.45 SS.seek 0ml @15:17:03.12 Exhaust.select :close @15:17:03.23 SSV.dial CBV @15:17:03.26 CBV.dial SSV @15:17:04.07 Allowing 130s for 25.0ml of remaining 925.0ml @15:17:04.08 Intake.select :open @15:17:06.25 Intake @14.9psia, vacuum from 9.4 to 12.4psi @15:17:06.27 SS.reconfigure :inhaling 2.17ml/s @15:17:06.32 SS.seek 25ml @15:17:21.27 Stroke completed -- waiting for pressure to equalize ... @15:17:21.28 SS.reconfigure :exhale @15:17:21.33 SS.hold @15:17:41.82 Intake.select :close @15:17:41.98 SSV.dial :exhaust @15:17:42.78 Exhaust.select :open @15:17:42.89 SS.seek 0ml @15:17:51.65 Exhaust.select :close @15:17:51.76 SSV.dial CBV @15:17:51.80 CBV.dial SSV @15:17:52.57 Allowing 130s for 25.0ml of remaining 900.0ml @15:17:52.58 Intake.select :open @15:17:54.75 Intake @14.9psia, vacuum from 9.4 to 12.4psi @15:17:54.76 SS.reconfigure :inhaling 2.17ml/s @15:17:54.81 SS.seek 25ml @15:18:09.91 Stroke completed -- waiting for pressure to equalize ... @15:18:09.92 SS.reconfigure :exhale @15:18:09.97 SS.hold @15:18:44.66 recoverPRVreagent08 @15:18:44.68 PTV.dial :mixing @15:18:45.40 PBV.dial :waste1 @15:18:45.78 delay 30 seconds @15:18:50.16 SS.reconfigure :default @15:18:50.21 SS.hold @15:18:50.28 Intake.select :close @15:18:50.44 ShallowSampler::Clogged in WCR -- Intake @15psia & Outlet @13psia won't equalize after 125.0ml @15:18:50.46 SSV.dial :exhaust @15:18:51.22 Exhaust.select :open @15:18:51.34 SS.reconfigure :exhale @15:18:51.39 SS.seek 0ml @15:19:00.19 Exhaust.select :close @15:19:00.30 Sampled 125.0ml @15:19:00.32 Drying puck before fixing for 4:00 @15:19:00.33 CBV.dial :waste2b @15:19:01.08 delay 1:00 @15:19:15.80 PBV.dial between PSV and waste1 @15:19:16.17 PSV.dial :air @15:19:16.63 PS.reconfigure :fast @15:19:17.12 PS.seek 2.5ml @15:19:40.39 PSV.dial :mixloop @15:19:40.84 delay 2 seconds @15:19:42.86 PSV.dial :air @15:19:43.31 delay 2 seconds @15:19:45.33 PSV.dial :mixloop @15:19:45.79 delay 2 seconds @15:19:47.81 PSV.dial :air @15:19:48.26 PS.seek 0ml @15:20:01.10 CSV.dial :air @15:20:01.57 CS.reconfigure :fast @15:20:01.62 CS.seek 10ml @15:20:11.52 washCycleDA #1 of 2 @15:20:11.53 PSR.select :air @15:20:11.55 PSV.dial PSR @15:20:12.86 Pulling 0.5ml of PSR.wash @15:20:12.87 PSR.select :wash @15:20:12.99 PS.reconfigure :default @15:20:13.04 PS.seek 0.5ml @15:20:18.92 delay 5 seconds @15:20:23.94 PSR.select :air @15:20:24.06 PS.seek 2ml @15:20:25.47 Pumping wcr puck from tube 2 up to 35psi... @15:20:25.49 CBV.dial SSV @15:20:26.74 CBV.dial between CSV and waste2b @15:20:27.70 CSV.dial between air and CTV @15:20:28.02 CS.seek 7.268ml @15:20:35.16 CTV.dial CSV,avoiding: :air @15:20:35.19 CSV.dial CTV @15:20:36.00 CS.reconfigure :pressurize @15:20:36.06 CS.seek 0ml @15:20:40.27 PTV.dial :puck @15:20:41.00 PSV.dial PTV @15:20:41.73 PBV.dial :waste1 @15:20:42.17 PS.seek 1.5ml @15:20:48.11 delay 3 seconds @15:20:51.13 PBV.dial between PSV and waste1 @15:20:51.51 PS.seek 0ml @15:20:55.18 CSV.dial :air @15:20:55.64 CS.reconfigure :fast @15:20:55.69 CS.seek 10ml @15:21:07.70 delay 2:00 @15:21:19.49 CSV.dial between air and CTV @15:21:19.94 CS.seek 2.895ml @15:21:37.43 CTV.dial CSV,avoiding: :air @15:21:37.46 CSV.dial CTV @15:21:37.78 CS.reconfigure :pressurize @15:21:37.84 CS.seek 0ml @15:21:39.55 CBV.dial :waste2b @15:21:39.88 CS.reconfigure :default @15:21:39.93 CS.seek 0ml @15:21:46.84 delay 1:00 @15:22:46.87 CTV.dial :air,avoiding: :intake @15:22:48.24 Fix #1 of 1 (with RNAlater) for 4:00 @15:22:48.25 CSV.dial CSR @15:22:48.68 Pulling 2ml of CSR.RNAlater @15:22:48.69 CSR.select :RNAlater @15:22:48.82 CS.seek 2ml @15:22:54.84 delay 5 seconds @15:22:59.86 CSR.select :air @15:22:59.98 CS.reconfigure :fast @15:23:00.03 CS.seek 10ml @15:23:07.73 recoverPSRreagent08 @15:23:07.74 PTV.dial :puck @15:23:07.81 PBV.dial :waste1 @15:23:08.20 delay 30 seconds @15:23:19.12 Pumping wcr puck from tube 2 up to 35psi... @15:23:19.14 CBV.dial SSV @15:23:20.39 CBV.dial between CSV and waste2b @15:23:21.35 CSV.dial between air and CTV @15:23:21.96 CS.seek 7.151ml @15:23:29.50 CTV.dial CSV,avoiding: :air @15:23:29.54 CSV.dial CTV @15:23:30.91 CS.reconfigure :pressurize @15:23:30.96 CS.seek 0ml @15:23:38.22 PBV.dial between PSV and waste1 @15:23:38.62 PSR.select :air @15:23:38.64 PSV.dial PSR @15:23:39.50 PS.reconfigure :fast @15:23:39.55 PS.seek 2.5ml @15:23:47.14 delay 4:00 @15:24:02.82 PSV.dial PTV @15:24:03.56 delay 5 seconds @15:24:08.58 PSV.dial PSR @15:24:09.43 delay 5 seconds @15:24:14.46 PSV.dial PTV @15:24:15.18 delay 5 seconds @15:24:20.20 PSV.dial :air @15:24:20.97 PS.seek 0ml @15:24:44.11 washCycleDA #2 of 2 @15:24:44.12 PSR.select :air @15:24:44.14 PSV.dial PSR @15:24:46.00 Pulling 0.5ml of PSR.wash @15:24:46.01 PSR.select :wash @15:24:46.13 PS.reconfigure :default @15:24:46.18 PS.seek 0.5ml @15:24:52.11 delay 5 seconds @15:24:57.13 PSR.select :air @15:24:57.28 PS.seek 2ml @15:25:13.45 PTV.dial :puck @15:25:13.46 PSV.dial PTV @15:25:14.21 PBV.dial :waste1 @15:25:14.67 PS.seek 1.5ml @15:25:20.67 delay 3 seconds @15:25:23.69 PBV.dial between PSV and waste1 @15:25:24.06 PS.seek 0ml @15:25:40.23 delay 2:00 @15:27:40.25 recoverPSRreagent08 @15:27:40.26 PTV.dial :puck @15:27:40.27 PBV.dial :waste1 @15:27:40.65 delay 30 seconds @15:27:47.17 CBV.dial :waste2b @15:27:47.50 CS.reconfigure :default @15:27:47.55 CS.seek 0ml @15:27:50.83 delay 1:00 @15:28:10.68 PBV.dial between PSV and waste1 @15:28:11.06 PSR.select :air @15:28:11.07 PSV.dial PSR @15:28:11.81 PS.reconfigure :fast @15:28:11.86 PS.seek 2.5ml @15:28:35.26 PSV.dial PTV @15:28:36.01 delay 5 seconds @15:28:41.03 PSV.dial PSR @15:28:41.88 delay 5 seconds @15:28:46.91 PSV.dial PTV @15:28:47.63 delay 5 seconds @15:28:50.86 CTV.dial :air,avoiding: :intake @15:28:52.65 PSV.dial :air @15:28:53.42 PS.seek 0ml @15:29:16.54 getPMLReagent08 @15:29:16.55 PRV.dial :air @15:29:17.01 delay 5 seconds @15:29:22.03 PTV.dial :PRVmixing @15:29:23.32 delay 5 seconds @15:29:28.34 PRV.dial :ab2 @15:29:28.79 PTV.dial :PRVmixing @15:29:28.87 PSV.dial :mixloop @15:29:29.34 PS.reconfigure :default @15:29:29.39 PS.seek 0.3ml @15:29:33.29 delay 2 seconds @15:29:35.31 PRV.dial :air @15:29:35.83 PS.seek 0.55ml @15:29:39.20 addPMLReagent08 @15:29:39.21 PSV.dial :air @15:29:39.67 PS.seek 2ml @15:29:55.29 PSV.dial :PML @15:29:55.76 PTV.dial :mixing @15:29:56.49 PBV.dial :waste1 @15:29:56.93 PS.seek 1.85ml @15:29:59.35 PBV.dial between PSV and waste1 @15:30:00.19 PS.seek 0ml @15:30:20.01 delay 2 seconds @15:30:22.03 delay 8:00 @15:38:22.06 recoverPRVreagent08 @15:38:22.07 PTV.dial :mixing @15:38:22.24 PBV.dial :waste1 @15:38:22.61 delay 30 seconds @15:38:52.64 PBV.dial between PSV and waste1 @15:38:53.02 PSV.dial :air @15:38:53.49 PS.reconfigure :fast @15:38:53.54 PS.seek 2.5ml @15:39:16.78 PSV.dial :mixloop @15:39:17.25 delay 2 seconds @15:39:19.27 PSV.dial :air @15:39:19.74 delay 2 seconds @15:39:21.75 PSV.dial :mixloop @15:39:22.22 delay 2 seconds @15:39:24.24 PSV.dial :air @15:39:24.71 PS.seek 0ml @15:39:48.00 washCycleDA #1 of 3 @15:39:48.01 PSR.select :air @15:39:48.03 PSV.dial PSR @15:39:49.34 Pulling 0.5ml of PSR.wash @15:39:49.35 PSR.select :wash @15:39:49.47 PS.reconfigure :default @15:39:49.52 PS.seek 0.5ml @15:39:55.50 delay 5 seconds @15:40:00.52 PSR.select :air @15:40:00.64 PS.seek 2ml @15:40:16.75 PTV.dial :puck @15:40:17.50 PSV.dial PTV @15:40:18.23 PBV.dial :waste1 @15:40:18.67 PS.seek 1.5ml @15:40:24.56 delay 3 seconds @15:40:27.58 PBV.dial between PSV and waste1 @15:40:27.96 PS.seek 0ml @15:40:44.13 delay 2:00 @15:42:44.15 recoverPSRreagent08 @15:42:44.16 PTV.dial :puck @15:42:44.23 PBV.dial :waste1 @15:42:44.61 delay 30 seconds @15:43:14.63 PBV.dial between PSV and waste1 @15:43:15.01 PSR.select :air @15:43:15.03 PSV.dial PSR @15:43:15.89 PS.reconfigure :fast @15:43:15.94 PS.seek 2.5ml @15:43:39.31 PSV.dial PTV @15:43:40.05 delay 5 seconds @15:43:45.07 PSV.dial PSR @15:43:45.92 delay 5 seconds @15:43:50.94 PSV.dial PTV @15:43:51.67 delay 5 seconds @15:43:56.69 PSV.dial :air @15:43:57.44 PS.seek 0ml @15:44:20.62 washCycleDA #2 of 3 @15:44:20.64 PSR.select :air @15:44:20.65 PSV.dial PSR @15:44:21.97 Pulling 0.5ml of PSR.wash @15:44:21.98 PSR.select :wash @15:44:22.09 PS.reconfigure :default @15:44:22.14 PS.seek 0.5ml @15:44:28.28 delay 5 seconds @15:44:33.30 PSR.select :air @15:44:33.42 PS.seek 2ml @15:44:50.00 PTV.dial :puck @15:44:50.01 PSV.dial PTV @15:44:50.75 PBV.dial :waste1 @15:44:51.18 PS.seek 1.5ml @15:44:57.09 delay 3 seconds @15:45:00.11 PBV.dial between PSV and waste1 @15:45:00.49 PS.seek 0ml @15:45:16.72 delay 2:00 @15:47:16.75 recoverPSRreagent08 @15:47:16.76 PTV.dial :puck @15:47:16.77 PBV.dial :waste1 @15:47:17.14 delay 30 seconds @15:47:47.16 PBV.dial between PSV and waste1 @15:47:47.54 PSR.select :air @15:47:47.56 PSV.dial PSR @15:47:48.31 PS.reconfigure :fast @15:47:48.36 PS.seek 2.5ml @15:48:11.71 PSV.dial PTV @15:48:12.46 delay 5 seconds @15:48:17.49 PSV.dial PSR @15:48:18.21 delay 5 seconds @15:48:23.24 PSV.dial PTV @15:48:23.98 delay 5 seconds @15:48:29.00 PSV.dial :air @15:48:29.77 PS.seek 0ml @15:48:53.22 washCycleDA #3 of 3 @15:48:53.23 PSR.select :air @15:48:53.24 PSV.dial PSR @15:48:54.54 Pulling 0.5ml of PSR.wash @15:48:54.55 PSR.select :wash @15:48:54.67 PS.reconfigure :default @15:48:54.72 PS.seek 0.5ml @15:49:00.65 delay 5 seconds @15:49:05.67 PSR.select :air @15:49:05.79 PS.seek 2ml @15:49:21.90 PTV.dial :puck @15:49:21.91 PSV.dial PTV @15:49:22.65 PBV.dial :waste1 @15:49:23.09 PS.seek 1.5ml @15:49:29.03 delay 3 seconds @15:49:32.05 PBV.dial between PSV and waste1 @15:49:32.42 PS.seek 0ml @15:49:48.56 delay 2:00 @15:51:48.59 recoverPSRreagent08 @15:51:48.60 PTV.dial :puck @15:51:48.61 PBV.dial :waste1 @15:51:48.98 delay 30 seconds @15:52:19.00 PBV.dial between PSV and waste1 @15:52:19.38 PSR.select :air @15:52:19.40 PSV.dial PSR @15:52:20.26 PS.reconfigure :fast @15:52:20.31 PS.seek 2.5ml @15:52:43.55 PSV.dial PTV @15:52:44.29 delay 5 seconds @15:52:49.31 PSV.dial PSR @15:52:50.16 delay 5 seconds @15:52:55.18 PSV.dial PTV @15:52:55.91 delay 5 seconds @15:53:00.93 PSV.dial :air @15:53:01.70 PS.seek 0ml @15:53:24.96 Gate.power :camera,:ON @15:53:24.97 Mixing DA Substrates @15:53:24.98 PSR.select :air @15:53:25.00 PSV.dial PSR @15:53:26.33 Pulling 0.25ml of PSR.sub1 @15:53:26.36 PSR.select :sub1 @15:53:27.60 PS.reconfigure :default @15:53:27.86 PS.seek 0.25ml @15:53:31.33 delay 5 seconds @15:53:36.36 Pulling 0.25ml of PSR.sub2 @15:53:36.37 PSR.select :sub2 @15:53:36.49 PS.seek 0.5ml @15:53:39.93 delay 5 seconds @15:53:44.95 PSR.select :air @15:53:45.06 PS.seek 2ml @15:54:01.18 PTV.dial :puck @15:54:01.19 PSV.dial PTV @15:54:01.93 PBV.dial :waste1 @15:54:02.36 PS.seek 1.5ml @15:54:08.37 delay 3 seconds @15:54:11.38 PBV.dial between PSV and waste1 @15:54:11.76 PS.seek 0ml @15:54:27.86 delay 10 seconds @15:54:37.89 recoverPSRreagent08 @15:54:37.90 PTV.dial :puck @15:54:37.92 PBV.dial :waste1 @15:54:38.29 delay 30 seconds @15:55:08.31 PBV.dial between PSV and waste1 @15:55:08.69 PSR.select :air @15:55:08.71 PSV.dial PSR @15:55:09.46 PS.reconfigure :fast @15:55:09.51 PS.seek 2.5ml @15:55:33.05 PSV.dial PTV @15:55:33.80 delay 5 seconds @15:55:38.82 PSV.dial PSR @15:55:39.57 delay 5 seconds @15:55:44.59 PSV.dial PTV @15:55:45.35 delay 5 seconds @15:55:50.37 PSV.dial :air @15:55:51.14 PS.seek 0ml @15:56:14.52 Additional air clear @15:56:14.53 PSR.select :air @15:56:14.55 PSV.dial PSR @15:56:15.86 Pulling 2ml of PSR.air @15:56:15.87 PSR.select :air @15:56:15.90 PS.reconfigure :default @15:56:15.94 PS.seek 2ml @15:56:37.27 delay 5 seconds @15:56:42.29 PTV.dial :puck @15:56:42.31 PSV.dial PTV @15:56:43.03 PBV.dial :waste1 @15:56:43.47 PS.seek 1.5ml @15:56:49.39 delay 3 seconds @15:56:52.41 PBV.dial between PSV and waste1 @15:56:52.79 delay 1 second @15:56:53.80 PSV.dial :waste1 @15:56:54.84 PS.seek 0ml @15:57:11.04 Processing Puck Heater at 28C before imaging @15:57:11.05 PH.coast @15:57:11.15 Imaging da puck in Processing Clamp while storing it to tube 1 @15:57:11.33 SC.reconfigure :tight @15:57:11.37 SC.seek 1 @15:57:14.99 SC.configure :limp @15:57:15.20 Elbow.reconfigure :tight @15:57:15.25 Elbow.seek 1 @15:57:17.48 Forearm.reconfigure :tight @15:57:17.53 Forearm.seek 1 + 800 counts @15:57:18.64 SE.reconfigure :pick @15:57:18.70 SE.seek :beamBreak @15:57:20.48 SE.configure :default @15:57:20.65 Elbow.reconfigure :limp @15:57:20.70 Forearm.reconfigure :loose @15:57:20.75 Forearm.seek 1 @15:57:21.14 Elbow.hold @15:57:21.20 Elbow.reconfigure :tight @15:57:21.30 SE.seek 13.1 Pucks below Top Plate @15:57:21.89 Forearm.force -20 @15:57:21.93 Forearm.seek 1 + 304 counts @15:57:22.34 Forearm.reconfigure :tight @15:57:22.39 Forearm.seek 1 + 800 counts @15:57:22.71 SE.seek 14.1 Pucks below Top Plate @15:57:23.36 Forearm.reconfigure :loose @15:57:23.41 Forearm.seek :retract @15:57:24.31 Elbow.seek PC @15:57:26.83 Elbow.reconfigure :loose @15:57:26.93 Forearm.seek PC @15:57:28.01 Hand.close @15:57:28.73 PC.seek :open @15:57:33.30 Forearm.seek :retract @15:57:34.36 Elbow.reconfigure :tight @15:57:34.41 Elbow.seek 1 @15:57:37.02 Forearm.reconfigure :tight @15:57:37.07 Forearm.seek 1 + 800 counts @15:57:38.10 Hand.open @15:57:38.92 SE.seek 13.86 Pucks below Top Plate @15:57:39.20 SE.reconfigure :seal @15:57:39.74 SE.seek 13.2 Pucks below Top Plate @15:57:40.28 Lights, camera, ACTION! @15:57:40.31 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da25sep3015h5ml.tif @15:58:00.10 Waiting >20 seconds for da to relinquish FlushPuck @15:58:01.00 SX HX9/16 (USB:04): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 17.407 seconds /var/log/esp/da25sep3015h5ml.tif: TIFF Upload Complete @15:58:01.04 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da25sep3015h5ml15s.tif @15:58:16.68 SX HX9/16 (USB:04): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da25sep3015h5ml15s.tif: TIFF Upload Complete @15:58:16.72 PuckCamera: snap -bin=2,2 21.0 /var/log/esp/midres/da25sep3015h5ml21s.tif @15:58:38.44 SX HX9/16 (USB:04): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21 seconds /var/log/esp/midres/da25sep3015h5ml21s.tif: TIFF Upload Complete @15:58:38.46 Gate.power :camera,:OFF @15:58:38.48 DAfans.email "17.407 sec auto-exposure",Subject: "da25sep3015h5ml images!" @15:58:38.95 Elbow.seek 1 @15:58:39.18 Forearm.seek 1 + 800 counts @15:58:39.28 SE.reconfigure :gently @15:58:39.33 SE.seek 13.92 Pucks below Top Plate @15:58:40.16 Elbow.reconfigure :limp @15:58:40.21 Forearm.reconfigure :loose @15:58:40.28 Forearm.seek 1 @15:58:40.67 Elbow.hold @15:58:40.74 Elbow.reconfigure :tight @15:58:40.80 SE.reconfigure :default @15:58:40.85 SE.seek 14.1 Pucks below Top Plate @15:58:41.27 Forearm.force -20 @15:58:41.42 Forearm.seek 1 + 303 counts @15:58:41.83 Forearm.reconfigure :tight @15:58:41.89 Forearm.seek 1 + 800 counts @15:58:42.30 SE.seek :down @15:58:43.58 Forearm.reconfigure :loose @15:58:43.72 Forearm.seek :retract @15:58:43.76 SE.stop @15:58:45.07 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:58:45.63 Elbow.seek FlushPuck::Garage @15:58:47.54 Elbow.reconfigure :loose @15:58:48.79 Forearm.seek :garage @15:58:49.81 Hand.close @15:58:50.87 Forearm.seek :retract @15:58:51.98 Elbow.reconfigure :tight @15:58:52.08 Elbow.seek PC @15:58:53.53 Elbow.reconfigure :loose @15:58:53.74 Forearm.reconfigure :tight @15:58:53.84 Forearm.seek PC @15:58:55.55 PC.seek :closed @15:59:00.09 Processing Clamp onSpring/offSpring for 12/26 samples, on/off torque 1.56 @15:59:00.27 Hand.open @15:59:01.16 Forearm.reconfigure :loose @15:59:01.31 Forearm.seek :retract @15:59:02.44 PSR.select :air @15:59:02.49 Storing wcr puck in Collection Clamp to tube 1 @15:59:02.55 PSV.dial PSR @15:59:03.22 Pulling 0.5ml of PSR.flush @15:59:03.30 PSR.select :flush @15:59:03.75 PS.seek 0.5ml @15:59:03.88 Elbow.reconfigure :tight @15:59:03.98 Elbow.seek 1 @15:59:05.93 upsync @15:59:06.68 Forearm.reconfigure :tight @15:59:06.74 Forearm.seek 1 + 800 counts @15:59:08.18 SE.reconfigure :pick @15:59:08.26 SE.seek :beamBreak @15:59:09.67 delay 5 seconds @15:59:09.96 SE.configure :default @15:59:10.13 Elbow.reconfigure :limp @15:59:10.18 Forearm.reconfigure :loose @15:59:10.23 Forearm.seek 1 @15:59:10.62 Elbow.hold @15:59:10.69 Elbow.reconfigure :tight @15:59:10.79 SE.seek 14.1 Pucks below Top Plate @15:59:11.33 Forearm.force -20 @15:59:11.38 Forearm.seek 1 + 312 counts @15:59:11.66 Forearm.reconfigure :tight @15:59:11.71 Forearm.seek 1 + 800 counts @15:59:12.05 SE.seek 15.1 Pucks below Top Plate @15:59:12.66 Forearm.reconfigure :loose @15:59:12.71 Forearm.seek :retract @15:59:13.64 Elbow.seek CC @15:59:14.69 PSR.select :air @15:59:14.82 PS.seek 2.5ml @15:59:15.21 Elbow.reconfigure :loose @15:59:15.31 Forearm.seek CC @15:59:16.34 Hand.close @15:59:17.11 CC.seek :open @15:59:21.33 Forearm.seek :retract @15:59:22.33 Elbow.reconfigure :tight @15:59:22.38 Elbow.seek 1 @15:59:24.57 Elbow.reconfigure :limp @15:59:24.63 Forearm.seek 1 @15:59:25.78 Elbow.hold @15:59:25.85 Elbow.reconfigure :tight @15:59:25.90 Hand.open @15:59:27.63 Forearm.force -20 @15:59:27.68 Forearm.seek 1 + 317 counts @15:59:28.04 Forearm.reconfigure :tight @15:59:28.09 Forearm.seek 1 + 800 counts @15:59:28.45 SE.seek :down @15:59:29.59 Forearm.reconfigure :loose @15:59:29.64 Forearm.seek :retract @15:59:29.72 SE.stop @15:59:30.62
Starting finalPurge @15:59:36.11 delay 1 second @15:59:37.13 PRV.dial :waste2,avoiding: :ab2 @15:59:38.99 PTV.dial :PRVmixing @15:59:40.27 PSV.dial :mixloop @15:59:41.40 PS.seek :empty @15:59:49.77
Waiting >20 seconds for psFlush to relinquish FlushPuck @16:00:07.98 PSR.select :air @16:00:08.01 PSV.dial PSR @16:00:09.03 Pulling 0.5ml of PSR.flush @16:00:09.04 PSR.select :flush @16:00:09.16 PS.seek 0.5ml @16:00:15.17 delay 5 seconds @16:00:20.19 PSR.select :air @16:00:20.31 PS.seek 2.5ml @16:00:41.64 delay 1 second @16:00:42.66 PBV.dial :waste1 @16:00:43.03 PTV.dial :mixing @16:00:43.77 PSV.dial :mixloop @16:00:44.82 PS.seek :empty @16:01:11.30 PSV.dial PSR @16:01:12.34 PSR.select :flush @16:01:12.46 PS.seek 1ml @16:01:23.51 PSR.select :air @16:01:23.63 PS.seek 2.5ml @16:01:39.80 PSV.dial PTV @16:01:40.61 PTV.dial :puck @16:01:41.34 PBV.dial :waste1 @16:01:41.43 PS.seek 0ml @16:02:07.73 PSV.dial PSR @16:02:08.59 PSR.select :flush @16:02:08.71 PS.seek 0.5ml @16:02:14.70 PSR.select :air @16:02:14.82 PS.seek 2.5ml @16:02:36.14 PSV.dial PTV @16:02:36.87 PBV.dial PSV @16:02:37.46 PS.seek 0ml @16:03:03.83 PSV.dial PBV @16:03:04.29 PS.seek 2.5ml @16:03:30.67 PSV.dial :waste1 @16:03:31.41 PS.seek 0ml @16:03:57.86 PSV.dial PSR @16:03:58.33 PSR.select :air @16:03:58.35 PS.seek 2.5ml @16:04:24.86 PSV.dial PTV @16:04:25.58 PBV.dial PSV @16:04:25.66 PS.seek 0ml @16:04:52.01 PSV.dial PBV @16:04:52.47 PS.seek 2.5ml @16:05:18.83 PSV.dial :waste1 @16:05:19.57 PS.seek 0ml @16:05:46.14 PSV.dial PSR @16:05:46.61 PSR.select :flush @16:05:46.73 PS.seek 1ml @16:05:57.79 PSR.select :air @16:05:57.91 PS.seek 2.5ml @16:06:14.07 PSV.dial PTV @16:06:14.79 PTV.dial :puck @16:06:14.87 PBV.dial :waste1 @16:06:15.46 PS.seek 0ml @16:06:41.79 PSV.dial PSR @16:06:42.53 PSR.select :flush @16:06:42.65 PS.seek 0.5ml @16:06:48.67 PSR.select :air @16:06:48.78 PS.seek 2.5ml @16:07:10.01 PSV.dial PTV @16:07:10.87 PBV.dial PSV @16:07:11.46 PS.seek 0ml @16:07:37.79 PSV.dial PBV @16:07:38.69 PS.seek 2.5ml @16:08:05.13 PSV.dial :waste1 @16:08:05.99 PS.seek 0ml @16:08:32.35 PSV.dial PSR @16:08:32.79 PSR.select :air @16:08:32.82 PS.seek 2.5ml @16:08:59.20 PSV.dial PTV @16:08:59.93 PBV.dial PSV @16:09:00.02 PS.seek 0ml @16:09:26.54 PSV.dial PBV @16:09:27.00 PS.seek 2.5ml @16:09:53.41 PSV.dial :waste1 @16:09:54.16 PS.seek 0ml @16:10:20.51
Loading FlushPuck into Collection Clamp from Processing Clamp @16:10:20.70 Elbow.seek PC @16:10:23.23 Elbow.reconfigure :loose @16:10:23.33 Forearm.seek PC @16:10:24.44 Hand.close @16:10:25.22 PC.seek :open @16:10:29.72 Forearm.seek :retract @16:10:30.74 Elbow.reconfigure :tight @16:10:30.78 Elbow.seek CC @16:10:34.32 Elbow.reconfigure :loose @16:10:34.42 Forearm.reconfigure :tight @16:10:34.47 Forearm.seek CC @16:10:35.79 CC.seek :closed @16:10:39.80 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.94 @16:10:39.87 Hand.open @16:10:40.73 Forearm.reconfigure :loose @16:10:40.78 Forearm.seek :retract @16:10:41.84 Flushing the Collection Valve::Manifold @16:10:41.97 CSV.dial CSR @16:10:42.41 Pulling 5ml of CSR.flush @16:10:42.42 CSR.select :flush @16:10:42.53 CS.seek 5ml @16:10:56.14 delay 5 seconds @16:11:01.16 CSR.select :air @16:11:01.33 CS.reconfigure :fast @16:11:01.38 CS.seek 7.5ml @16:11:08.05 CSV.dial :waste @16:11:09.32 CS.reconfigure :default @16:11:09.37 CS.seek 0ml @16:11:29.33 Flushing lower manifold's waste1 port @16:11:29.45 CSV.dial :flush @16:11:29.90 CS.reconfigure :fast @16:11:29.94 CS.seek 5ml @16:11:42.27 delay 5 seconds @16:11:47.29 CSV.dial :air @16:11:47.78 CS.seek 7ml @16:11:53.17 CSV.dial CTV @16:11:53.20
CTV.dial CSV @16:11:54.61 CBV.dial :waste1 @16:11:55.37 CS.seek 0ml @16:12:12.17 Flushing lower manifold's waste2 port @16:12:12.30 CSV.dial :flush @16:12:13.04 CS.seek 5ml @16:12:25.33 delay 5 seconds @16:12:30.35 CSV.dial :air @16:12:30.87 CS.seek 7ml @16:12:36.39 CSV.dial CTV @16:12:36.43
CTV.dial CSV @16:12:36.86 CBV.dial :waste2 @16:12:37.91 CS.seek 0ml @16:12:54.64 Flushing lower manifold's waste2b port @16:12:54.76 CSV.dial :flush @16:12:55.51 CS.seek 5ml @16:13:07.80 delay 5 seconds @16:13:12.82 CSV.dial :air @16:13:13.34 CS.seek 7ml @16:13:18.87 CSV.dial CTV @16:13:18.89
CTV.dial CSV @16:13:19.34 CBV.dial :waste2b @16:13:20.38 CS.seek 0ml @16:13:37.17 Disinfecting external Intake with kill @16:13:37.29 CSV.dial CSR @16:13:37.73 Pulling 8ml of CSR.kill @16:13:37.74 CSR.select :kill @16:13:37.86 CS.seek 8ml @16:13:56.98 delay 5 seconds @16:14:02.00 CSR.select :air @16:14:02.12 CS.seek 10ml @16:14:07.62 CSV.dial CBV @16:14:07.64
CBV.dial CSV @16:14:08.67 CTV.dial :intake @16:14:09.45 Intake.select :open @16:14:09.56 CS.seek 2ml @16:14:28.58 Intake.select :close @16:14:28.68 CTV.dial :air @16:14:29.46 CS.reconfigure :default @16:14:29.50 CS.seek 0ml @16:14:35.45 CSV.dial CSR @16:14:36.58 Pulling 5ml of CSR.flush @16:14:36.59 CSR.select :flush @16:14:36.71 CS.seek 5ml @16:14:50.29 delay 5 seconds @16:14:55.31 CSR.select :air @16:14:55.43 CS.seek 10ml @16:15:09.14 CSV.dial CBV @16:15:10.13 CS.seek 0ml @16:15:36.58 Garaging FlushPuck in Collection Clamp @16:15:36.65 Elbow.reconfigure :tight @16:15:36.69 Elbow.seek CC @16:15:36.81 Elbow.reconfigure :loose @16:15:36.92 Forearm.seek CC @16:15:37.96 Hand.close @16:15:38.72 CC.seek :open @16:15:42.82 Forearm.seek :retract @16:15:43.87 Elbow.reconfigure :tight @16:15:43.92 Elbow.seek :garage @16:15:46.75 Elbow.reconfigure :loose @16:15:46.85 Forearm.reconfigure :tight @16:15:46.90 Forearm.seek :garage @16:15:48.05 Hand.open @16:15:48.90 Forearm.reconfigure :loose @16:15:48.94 Forearm.seek :retract @16:15:50.12 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 14:24:51 @16:15:50.17 Asleep after completing DA_WCR_093025 assays @16:15:50.19 GoodNews.email "{1 => 16,\n 2 => 6,\n 3 => 22,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@16:14:19, 40% flow, 22.2C, 17% humidity, 14.7psia, 13.70V, 0.516A avg, 65.807Ah, 7.07W\nSampled 125.0ml",Subject: "Asleep after completing DA_WCR_093025 assays" @16:15:54.48 upsync + ESPwhich DA_WCR_100125.rb + fn=DA_WCR_100125.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_100125.rb + [ -f /home/esp/esp2/mission/DA_WCR_100125.rb ] + realpath /home/esp/esp2/mission/DA_WCR_100125.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_100125.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_100125.rb @15:46:57.91UTC01-Oct-25
ESP2 v3.42 I2C Gateway v3.20 for core bus @15:47:00.61 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @15:47:04.53 Polling Can every 10:00 @15:47:04.60 Cleanly exited at 14:56:52UTC01-Oct-25 @15:47:04.64 Loading /home/esp/esp2local/mission/DA_WCR_100125.rb mission @15:47:04.69 GoodNews.email "Starting DA_WCR_100125 w/pucks in tube 2\n{1 => 16,\n 2 => 6,\n 3 => 22,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@15:47:04, 40% flow, 19.7C, 18% humidity, 14.6psia, 13.75V, 0.328A avg, 71.579Ah, 4.51W",Subject: "DA_WCR_100125 begun" @15:47:04.72
Preparing to sleep until + 00:00:05 @15:47:04.77 Asleep til + 00:00:05 @15:47:04.79 Continuing at 15:47 @15:47:05.07 Gate.power :core,:OFF @15:47:05.09 Gate.power :raw,:OFF @15:47:05.10 Shutdown @15:47:07.30 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_100125.rb:14\n{1 => 16,\n 2 => 6,\n 3 => 22,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@15:47:04, 40% flow, 19.7C, 18% humidity, 14.6psia, 13.75V, 0.334A avg, 71.579Ah, 4.59W",Subject: "Asleep until 15:47" @15:47:08.77 upsync @15:47:09.68
Powering core bus @15:47:11.13 upsync @15:47:14.83
Gate.power :raw,:ON @15:47:15.65 CS.configure :default @15:47:15.71 PS.configure :default @15:47:15.76 SS.configure :default @15:47:15.81 CC.configure :default @15:47:15.86 PC.configure :default @15:47:15.93 Forearm.configure :loose @15:47:15.98 Elbow.configure :loose @15:47:16.06 SC.configure :loose @15:47:16.11 SE.configure :default @15:47:16.16 CH.configure :default @15:47:16.19 PH.configure :default @15:47:16.23 All dwarf controllers running firmware version 3.85 @15:47:16.34 Valve.reset @15:47:16.98 Intake.select :close @15:47:17.01 PSV.dial :air @15:47:17.04 Exhaust.select :close @15:47:17.09 CTV.dial :air @15:47:17.20
Forearm.home.seek :retract @15:47:17.26 CSV.dial :waste1 @15:47:17.71 SSV.dial :spit,avoiding: :exhaust @15:47:18.11 PS.home.seek 0ml @15:47:18.33 Hand.close @15:47:18.35
Elbow.reconfigure :loose @15:47:18.45 Elbow.jog 1500 @15:47:18.59 CS.reconfigure :fast @15:47:18.63 SS.home.seek 0ml @15:47:18.72 CS.home.seek 0ml @15:47:19.14
Elbow.reconfigure :loose @15:47:19.19 Elbow.home.seek :clear @15:47:22.43 SE.home.seek :down @15:47:23.11 SC.reconfigure :tight @15:47:23.16 SC.home.seek :initial @15:47:27.22 PS.stop @15:47:33.19
SC.configure :limp @15:47:33.24 Forearm.seek :retract @15:47:33.47 Elbow.seek :hammer @15:47:34.51 Forearm.reconfigure :tight @15:47:34.56 Forearm.seek retracted @15:47:34.94 PC.home.seek :open @15:47:34.95
CC.home.seek :open @15:47:38.17 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @15:47:38.20 Notice.email "At 15:47:38 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_100125.rb:14\n{1 => 16,\n 2 => 6,\n 3 => 22,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@15:47:38, 40% flow, 19.6C, 19% humidity, 14.6psia, 13.72V, 0.259A avg, 71.582Ah, 3.55W",Subject: "Started DA_WCR_100125 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @15:47:38.23
Starting initialPurge @15:47:38.24 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:47:38.30 Hand.open @15:47:39.16 Elbow.reconfigure :tight @15:47:39.20 Elbow.seek FlushPuck::Garage @15:47:41.03 Elbow.reconfigure :loose @15:47:41.14 Forearm.reconfigure :loose @15:47:41.19 Forearm.seek :garage @15:47:41.91 upsync @15:47:42.20
Hand.close @15:47:43.12 Forearm.seek :retract @15:47:44.25 Elbow.reconfigure :tight @15:47:44.30 Elbow.seek CC @15:47:47.13 Elbow.reconfigure :loose @15:47:47.23 Forearm.reconfigure :tight @15:47:47.28 Forearm.seek CC @15:47:48.44 CC.seek :closed @15:47:52.44 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.04 @15:47:52.52 Hand.open @15:47:53.31 Forearm.reconfigure :loose @15:47:53.36 Forearm.seek :retract @15:47:54.39 Flushing the Collection Valve::Manifold @15:47:54.52 CSV.dial CSR @15:47:55.79 Pulling 5ml of CSR.flush @15:47:55.80 CSR.select :flush @15:47:55.92 CS.reconfigure :default @15:47:55.97 CS.seek 5ml @15:48:10.06 delay 5 seconds @15:48:15.07 CSR.select :air @15:48:15.24 CS.reconfigure :fast @15:48:15.29 CS.seek 7.5ml @15:48:21.93 CSV.dial :waste @15:48:23.33 CS.reconfigure :default @15:48:23.37 CS.seek 0ml @15:48:43.37 Purging external Intake @15:48:43.49 CSV.dial :flush @15:48:43.95 CS.reconfigure :fast @15:48:44.00 CS.seek 10ml @15:49:08.02 delay 5 seconds @15:49:13.05 CSV.dial CBV @15:49:13.08
CBV.dial CSV @15:49:13.82 CTV.dial :intake @15:49:14.59 Intake.select :open @15:49:14.70 CS.seek 0ml @15:49:38.43 Intake.select :close @15:49:38.53 CTV.dial :air @15:49:39.31 Flushing upper manifold @15:49:39.43 CSV.dial :flush @15:49:40.28 CS.seek 5ml @15:49:52.52 delay 5 seconds @15:49:57.54 CSV.dial :air @15:49:58.18 CS.seek 6.999ml @15:50:03.68 CSV.dial CBV @15:50:03.72
CBV.dial CSV @15:50:04.70 CTV.dial :air @15:50:04.78 CS.seek 0ml @15:50:21.46 delay 7 seconds @15:50:28.49 CS.seek 3ml @15:50:36.30 CSV.dial :waste @15:50:36.76 CS.seek 0ml @15:50:44.63 CSV.dial :flush @15:50:45.08 CS.seek 3ml @15:50:52.80 delay 5 seconds @15:50:57.82 CSV.dial :air @15:50:58.32 CS.seek 5.001ml @15:51:03.81 CSV.dial :air @15:51:04.00 CS.seek 0ml @15:51:16.21 delay 7 seconds @15:51:23.24 CS.seek 3ml @15:51:30.96 CSV.dial :waste @15:51:31.67 CS.seek 0ml @15:51:39.40 Garaging FlushPuck in Collection Clamp @15:51:39.47 Elbow.reconfigure :tight @15:51:39.51 Elbow.seek CC @15:51:39.63 Elbow.reconfigure :loose @15:51:39.74 Forearm.seek CC @15:51:40.71 Hand.close @15:51:41.49 CC.seek :open @15:51:45.98 Forearm.seek :retract @15:51:47.01 Elbow.reconfigure :tight @15:51:47.06 Elbow.seek :garage @15:51:49.92 Elbow.reconfigure :loose @15:51:50.02 Forearm.reconfigure :tight @15:51:50.07 Forearm.seek :garage @15:51:51.10 Hand.open @15:51:51.93 Forearm.reconfigure :loose @15:51:51.98 Forearm.seek :retract @15:51:53.01 da sampling at most 1000ml, wcr at most 1000ml @15:51:53.05 Loading sh1 puck from tube 2 into Collection Clamp @15:51:53.07 DAfans.email "Started da in tube 2 at 15:51:53UTC01-Oct-25\n{1 => 16,\n 2 => 6,\n 3 => 22,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@15:47:38, 40% flow, 19.6C, 19% humidity, 14.6psia, 13.72V, 0.259A avg, 71.582Ah, 3.55W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @15:51:53.34
SC.reconfigure :tight @15:51:53.40 SC.seek 2 @15:51:56.91 upsync @15:52:00.22
SC.configure :limp @15:52:00.45 Elbow.reconfigure :tight @15:52:00.50 Elbow.seek 2 @15:52:02.45 Forearm.reconfigure :tight @15:52:02.51 Forearm.seek 2 + 800 counts @15:52:03.52 SE.reconfigure :pick @15:52:03.58 SE.seek :beamBreak @15:52:06.02 SE.configure :default @15:52:06.19 Elbow.seek 2 @15:52:06.37 Forearm.seek 2 + 300 counts @15:52:06.58 Forearm.reconfigure :loose @15:52:06.63 Hand.close @15:52:07.39 SE.seek :down @15:52:07.59
Forearm.seek :retract @15:52:08.91 Elbow.seek CC @15:52:09.72 SE.stop @15:52:10.52
Elbow.reconfigure :loose @15:52:10.62 Forearm.reconfigure :tight @15:52:10.67 Forearm.seek CC @15:52:11.98 CC.seek :closed @15:52:15.99 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.12 @15:52:16.00 Hand.open @15:52:16.81 Forearm.reconfigure :loose @15:52:16.85 Forearm.seek :retract @15:52:17.98 collectSample 1000ml w/bubble point of 24psi @15:52:17.99 Priming 7ml @15:52:18.00 Intake.select :close @15:52:18.01 Exhaust.select :close @15:52:18.02 SS.hold @15:52:18.24 Calibrating pressure and temperature sensors @15:52:18.26 CTV.dial :air @15:52:18.28 SSV.dial :spit,avoiding: :exhaust @15:52:18.30 SS.reconfigure :fast @15:52:18.34 SS.seek 5ml @15:52:21.39 SSV.dial CBV @15:52:21.43
CBV.dial SSV @15:52:22.78 delay 3.8 seconds @15:52:26.71 Sensor limits: Intake max 104.0psia; Outlet max 113.0psia @15:52:26.72 SSV.dial :spit @15:52:28.07 SS.seek 0ml @15:52:30.93 SSV.dial CBV @15:52:32.87 CTV.dial :intake @15:52:33.63 SSV.dial CBV @15:52:33.66
CBV.dial SSV @15:52:33.80 Allowing 40s for 7.0ml of remaining 7.0ml @15:52:33.81 Intake.select :open @15:52:35.98 Intake @14.9psia, vacuum from 9.4 to 12.4psi @15:52:35.99 SS.reconfigure :inhaling 2.17ml/s @15:52:36.04 SS.seek 7ml @15:52:41.28 Stroke completed -- waiting for pressure to equalize ... @15:52:41.29 SS.reconfigure :exhale @15:52:41.33 SS.hold @15:52:44.30 Intake.select :close @15:52:44.45 SSV.dial :exhaust @15:52:45.23 Exhaust.select :open @15:52:45.35 SS.seek 0ml @15:52:49.45 Exhaust.select :close @15:52:49.56 Sampling 1000.0ml @15:52:49.57 Intake.select :close @15:52:49.58 Exhaust.select :close @15:52:49.59 SS.reconfigure :default @15:52:49.64 SS.hold @15:52:49.85 CTV.dial :intake @15:52:49.93 SSV.dial CBV @15:52:49.97
CBV.dial SSV @15:52:50.73 Allowing 130s for 25.0ml of remaining 1000.0ml @15:52:50.74 Intake.select :open @15:52:52.91 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:52:52.92 SS.reconfigure :inhaling 2.17ml/s @15:52:52.97 SS.seek 25ml @15:53:06.67 Stroke completed -- waiting for pressure to equalize ... @15:53:06.68 SS.reconfigure :exhale @15:53:06.72 SS.hold @15:53:13.21 Intake.select :close @15:53:13.36 SSV.dial :exhaust @15:53:14.12 Exhaust.select :open @15:53:14.24 SS.seek 0ml @15:53:23.50 Exhaust.select :close @15:53:23.61 SSV.dial CBV @15:53:23.63
CBV.dial SSV @15:53:24.40 Allowing 130s for 25.0ml of remaining 975.0ml @15:53:24.41 Intake.select :open @15:53:26.57 Intake @14.5psia, vacuum from 9.0 to 12.0psi @15:53:26.59 SS.reconfigure :inhaling 2.17ml/s @15:53:26.64 SS.seek 25ml @15:53:40.56 Stroke completed -- waiting for pressure to equalize ... @15:53:40.57 SS.reconfigure :exhale @15:53:40.97 SS.hold @15:53:47.54 Intake.select :close @15:53:47.69 SSV.dial :exhaust @15:53:48.45 Exhaust.select :open @15:53:48.56 SS.seek 0ml @15:53:57.84 Exhaust.select :close @15:53:57.95 SSV.dial CBV @15:53:57.98
CBV.dial SSV @15:53:58.75 Allowing 130s for 25.0ml of remaining 950.0ml @15:53:58.76 Intake.select :open @15:54:00.92 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:54:00.94 SS.reconfigure :inhaling 2.17ml/s @15:54:00.99 SS.seek 25ml @15:54:14.88 Stroke completed -- waiting for pressure to equalize ... @15:54:14.89 SS.reconfigure :exhale @15:54:14.94 SS.hold @15:54:22.43 Intake.select :close @15:54:22.59 SSV.dial :exhaust @15:54:23.35 Exhaust.select :open @15:54:23.47 SS.seek 0ml @15:54:32.73 Exhaust.select :close @15:54:32.84 SSV.dial CBV @15:54:32.88
CBV.dial SSV @15:54:33.62 Allowing 130s for 25.0ml of remaining 925.0ml @15:54:33.63 Intake.select :open @15:54:35.80 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:54:35.81 SS.reconfigure :inhaling 2.17ml/s @15:54:35.86 SS.seek 25ml @15:54:50.13 Stroke completed -- waiting for pressure to equalize ... @15:54:50.14 SS.reconfigure :exhale @15:54:50.19 SS.hold @15:54:56.68 Intake.select :close @15:54:56.83 SSV.dial :exhaust @15:54:57.60 Exhaust.select :open @15:54:57.71 SS.seek 0ml @15:55:06.96 Exhaust.select :close @15:55:07.07 SSV.dial CBV @15:55:07.11
CBV.dial SSV @15:55:07.85 Allowing 130s for 25.0ml of remaining 900.0ml @15:55:07.87 Intake.select :open @15:55:10.04 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:55:10.05 SS.reconfigure :inhaling 2.17ml/s @15:55:10.10 SS.seek 25ml @15:55:24.02 Stroke completed -- waiting for pressure to equalize ... @15:55:24.03 SS.reconfigure :exhale @15:55:24.08 SS.hold @15:55:31.79 Intake.select :close @15:55:31.94 SSV.dial :exhaust @15:55:32.71 Exhaust.select :open @15:55:32.82 SS.seek 0ml @15:55:41.68 Exhaust.select :close @15:55:41.79 SSV.dial CBV @15:55:41.81
CBV.dial SSV @15:55:42.60 Allowing 130s for 25.0ml of remaining 875.0ml @15:55:42.61 Intake.select :open @15:55:44.77 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:55:44.79 SS.reconfigure :inhaling 2.17ml/s @15:55:44.84 SS.seek 25ml @15:55:59.15 Stroke completed -- waiting for pressure to equalize ... @15:55:59.16 SS.reconfigure :exhale @15:55:59.20 SS.hold @15:56:08.18 Intake.select :close @15:56:08.34 SSV.dial :exhaust @15:56:09.10 Exhaust.select :open @15:56:09.21 SS.seek 0ml @15:56:18.05 Exhaust.select :close @15:56:18.16 SSV.dial CBV @15:56:18.19
CBV.dial SSV @15:56:18.96 Allowing 130s for 25.0ml of remaining 850.0ml @15:56:18.97 Intake.select :open @15:56:21.14 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:56:21.16 SS.reconfigure :inhaling 2.17ml/s @15:56:21.20 SS.seek 25ml @15:56:35.52 Stroke completed -- waiting for pressure to equalize ... @15:56:35.53 SS.reconfigure :exhale @15:56:35.58 SS.hold @15:56:44.13 Intake.select :close @15:56:44.28 SSV.dial :exhaust @15:56:45.04 Exhaust.select :open @15:56:45.15 SS.seek 0ml @15:56:54.38 Exhaust.select :close @15:56:54.49 SSV.dial CBV @15:56:54.53
CBV.dial SSV @15:56:55.27 Allowing 130s for 25.0ml of remaining 825.0ml @15:56:55.28 Intake.select :open @15:56:57.44 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:56:57.46 SS.reconfigure :inhaling 2.17ml/s @15:56:57.51 SS.seek 25ml @15:57:11.47 Stroke completed -- waiting for pressure to equalize ... @15:57:11.48 SS.reconfigure :exhale @15:57:11.53 SS.hold @15:57:21.46 Intake.select :close @15:57:21.62 SSV.dial :exhaust @15:57:22.38 Exhaust.select :open @15:57:22.49 SS.seek 0ml @15:57:31.68 Exhaust.select :close @15:57:31.79 SSV.dial CBV @15:57:31.81
CBV.dial SSV @15:57:32.60 Allowing 130s for 25.0ml of remaining 800.0ml @15:57:32.61 Intake.select :open @15:57:34.78 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:57:34.79 SS.reconfigure :inhaling 2.17ml/s @15:57:34.84 SS.seek 25ml @15:57:49.16 Stroke completed -- waiting for pressure to equalize ... @15:57:49.17 SS.reconfigure :exhale @15:57:49.22 SS.hold @15:57:59.00 Intake.select :close @15:57:59.15 SSV.dial :exhaust @15:57:59.91 Exhaust.select :open @15:58:00.03 SS.seek 0ml @15:58:08.87 Exhaust.select :close @15:58:08.98 SSV.dial CBV @15:58:09.00
CBV.dial SSV @15:58:09.80 Allowing 130s for 25.0ml of remaining 775.0ml @15:58:09.82 Intake.select :open @15:58:11.98 Intake @14.6psia, vacuum from 9.1 to 12.1psi @15:58:12.00 SS.reconfigure :inhaling 2.17ml/s @15:58:12.05 SS.seek 25ml @15:58:26.47 Stroke completed -- waiting for pressure to equalize ... @15:58:26.48 SS.reconfigure :exhale @15:58:26.53 SS.hold @15:58:36.21 Intake.select :close @15:58:36.36 SSV.dial :exhaust @15:58:37.13 Exhaust.select :open @15:58:37.25 SS.seek 0ml @15:58:46.40 Exhaust.select :close @15:58:46.51 SSV.dial CBV @15:58:46.53
CBV.dial SSV @15:58:47.32 Allowing 130s for 25.0ml of remaining 750.0ml @15:58:47.33 Intake.select :open @15:58:49.92 Intake @14.7psia, vacuum from 9.2 to 12.2psi @15:58:49.93 SS.reconfigure :inhaling 2.17ml/s @15:58:49.98 SS.seek 25ml @15:59:04.24 Stroke completed -- waiting for pressure to equalize ... @15:59:04.25 SS.reconfigure :exhale @15:59:04.30 SS.hold @15:59:15.45 Intake.select :close @15:59:15.60 SSV.dial :exhaust @15:59:16.37 Exhaust.select :open @15:59:16.48 SS.seek 0ml @15:59:25.35 Exhaust.select :close @15:59:25.46 SSV.dial CBV @15:59:25.48
CBV.dial SSV @15:59:26.27 Allowing 130s for 25.0ml of remaining 725.0ml @15:59:26.28 Intake.select :open @15:59:28.45 Intake @14.8psia, vacuum from 9.3 to 12.3psi @15:59:28.46 SS.reconfigure :inhaling 2.17ml/s @15:59:28.51 SS.seek 25ml @15:59:43.14 Stroke completed -- waiting for pressure to equalize ... @15:59:43.15 SS.reconfigure :exhale @15:59:43.20 SS.hold @15:59:55.86 Intake.select :close @15:59:56.02 SSV.dial :exhaust @15:59:56.77 Exhaust.select :open @15:59:56.88 SS.seek 0ml @16:00:05.72 Exhaust.select :close @16:00:05.83 SSV.dial CBV @16:00:05.86
CBV.dial SSV @16:00:06.65 Allowing 130s for 25.0ml of remaining 700.0ml @16:00:06.66 Intake.select :open @16:00:08.82 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:00:08.84 SS.reconfigure :inhaling 2.17ml/s @16:00:08.89 SS.seek 25ml @16:00:23.21 Stroke completed -- waiting for pressure to equalize ... @16:00:23.22 SS.reconfigure :exhale @16:00:23.26 SS.hold @16:00:37.54 Intake.select :close @16:00:37.69 SSV.dial :exhaust @16:00:38.47 Exhaust.select :open @16:00:38.58 SS.seek 0ml @16:00:47.30 Exhaust.select :close @16:00:47.41 SSV.dial CBV @16:00:47.43
CBV.dial SSV @16:00:48.25 Allowing 130s for 25.0ml of remaining 675.0ml @16:00:48.27 Intake.select :open @16:00:50.43 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:00:50.45 SS.reconfigure :inhaling 2.17ml/s @16:00:50.50 SS.seek 25ml @16:01:04.81 Stroke completed -- waiting for pressure to equalize ... @16:01:04.82 SS.reconfigure :exhale @16:01:04.87 SS.hold @16:01:21.13 Intake.select :close @16:01:21.66 SSV.dial :exhaust @16:01:22.43 Exhaust.select :open @16:01:22.54 SS.seek 0ml @16:01:31.38 Exhaust.select :close @16:01:31.49 SSV.dial CBV @16:01:31.52
CBV.dial SSV @16:01:32.30 Allowing 130s for 25.0ml of remaining 650.0ml @16:01:32.31 Intake.select :open @16:01:34.47 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:01:34.49 SS.reconfigure :inhaling 2.17ml/s @16:01:34.54 SS.seek 25ml @16:01:48.94 Stroke completed -- waiting for pressure to equalize ... @16:01:48.95 SS.reconfigure :exhale @16:01:48.99 SS.hold @16:02:05.29 Intake.select :close @16:02:05.44 SSV.dial :exhaust @16:02:06.21 Exhaust.select :open @16:02:06.32 SS.seek 0ml @16:02:15.60 Exhaust.select :close @16:02:15.70 SSV.dial CBV @16:02:15.73
CBV.dial SSV @16:02:16.50 Allowing 130s for 25.0ml of remaining 625.0ml @16:02:16.51 Intake.select :open @16:02:18.68 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:02:18.70 SS.reconfigure :inhaling 2.17ml/s @16:02:19.17 SS.seek 25ml @16:02:33.25 Stroke completed -- waiting for pressure to equalize ... @16:02:33.26 SS.reconfigure :exhale @16:02:33.31 SS.hold @16:02:51.67 Intake.select :close @16:02:51.82 SSV.dial :exhaust @16:02:52.58 Exhaust.select :open @16:02:52.69 SS.seek 0ml @16:03:01.56 Exhaust.select :close @16:03:01.67 SSV.dial CBV @16:03:01.70
CBV.dial SSV @16:03:02.47 Allowing 130s for 25.0ml of remaining 600.0ml @16:03:02.48 Intake.select :open @16:03:04.64 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:03:04.66 SS.reconfigure :inhaling 2.17ml/s @16:03:04.71 SS.seek 25ml @16:03:16.79 Vacuum too high -- waiting up to 40 seconds @16:03:16.80 SS.reconfigure :exhale @16:03:16.86 SS.hold @16:03:27.25 Intake @12.7psia, vacuum from 7.2 to 10.2psi @16:03:27.27 SS.reconfigure :inhaling 1.95ml/s @16:03:27.32 SS.seek 25ml @16:03:30.53 Stroke completed -- waiting for pressure to equalize ... @16:03:30.54 SS.reconfigure :exhale @16:03:30.59 SS.hold @16:03:40.34 Intake.select :close @16:03:40.50 SSV.dial :exhaust @16:03:41.34 Exhaust.select :open @16:03:41.45 SS.seek 0ml @16:03:50.59 Exhaust.select :close @16:03:50.70 SSV.dial CBV @16:03:50.73
CBV.dial SSV @16:03:51.50 Allowing 130s for 25.0ml of remaining 575.0ml @16:03:51.51 Intake.select :open @16:03:53.68 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:03:53.69 SS.reconfigure :inhaling 1.95ml/s @16:03:53.74 SS.seek 25ml @16:04:03.01 Vacuum too high -- waiting up to 40 seconds @16:04:03.02 SS.reconfigure :exhale @16:04:03.08 SS.hold @16:04:10.88 Intake @13.0psia, vacuum from 7.5 to 10.5psi @16:04:10.90 SS.reconfigure :inhaling 1.77ml/s @16:04:10.95 SS.seek 25ml @16:04:18.45 Vacuum too high -- waiting up to 40 seconds @16:04:18.46 SS.reconfigure :exhale @16:04:18.51 SS.hold @16:04:28.85 Intake @13.9psia, vacuum from 8.4 to 11.4psi @16:04:28.86 SS.reconfigure :inhaling 1.59ml/s @16:04:28.91 SS.seek 25ml @16:04:29.14 Stroke completed -- waiting for pressure to equalize ... @16:04:29.15 SS.reconfigure :exhale @16:04:29.20 SS.hold @16:04:32.72 Intake.select :close @16:04:32.87 SSV.dial :exhaust @16:04:33.64 Exhaust.select :open @16:04:33.75 SS.seek 0ml @16:04:42.91 Exhaust.select :close @16:04:43.02 SSV.dial CBV @16:04:43.04
CBV.dial SSV @16:04:43.83 Allowing 130s for 25.0ml of remaining 550.0ml @16:04:43.84 Intake.select :open @16:04:46.00 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:04:46.02 SS.reconfigure :inhaling 1.59ml/s @16:04:46.07 SS.seek 25ml @16:04:57.66 Vacuum too high -- waiting up to 40 seconds @16:04:57.68 SS.reconfigure :exhale @16:04:57.73 SS.hold @16:05:05.47 Intake @13.1psia, vacuum from 7.6 to 10.6psi @16:05:05.49 SS.reconfigure :inhaling 1.43ml/s @16:05:05.53 SS.seek 25ml @16:05:11.76 Vacuum too high -- waiting up to 40 seconds @16:05:11.77 SS.reconfigure :exhale @16:05:11.82 SS.hold @16:05:21.77 Intake @13.4psia, vacuum from 7.9 to 10.9psi @16:05:21.79 SS.reconfigure :inhaling 1.29ml/s @16:05:21.83 SS.seek 25ml @16:05:24.03 Stroke completed -- waiting for pressure to equalize ... @16:05:24.04 SS.reconfigure :exhale @16:05:24.08 SS.hold @16:05:29.14 Intake.select :close @16:05:29.30 SSV.dial :exhaust @16:05:30.06 Exhaust.select :open @16:05:30.18 SS.seek 0ml @16:05:39.40 Exhaust.select :close @16:05:39.51 SSV.dial CBV @16:05:39.53
CBV.dial SSV @16:05:40.34 Allowing 130s for 25.0ml of remaining 525.0ml @16:05:40.35 Intake.select :open @16:05:42.52 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:05:42.53 SS.reconfigure :inhaling 1.29ml/s @16:05:42.58 SS.seek 25ml @16:05:53.32 Vacuum too high -- waiting up to 40 seconds @16:05:53.33 SS.reconfigure :exhale @16:05:53.38 SS.hold @16:06:00.02 Intake @13.6psia, vacuum from 8.1 to 11.1psi @16:06:00.04 SS.reconfigure :inhaling 1.16ml/s @16:06:00.09 SS.seek 25ml @16:06:06.97 Vacuum too high -- waiting up to 40 seconds @16:06:06.98 SS.reconfigure :exhale @16:06:07.04 SS.hold @16:06:16.04 Intake @13.8psia, vacuum from 8.3 to 11.3psi @16:06:16.06 SS.reconfigure :inhaling 1.04ml/s @16:06:16.11 SS.seek 25ml @16:06:22.60 Stroke completed -- waiting for pressure to equalize ... @16:06:22.62 SS.reconfigure :exhale @16:06:22.66 SS.hold @16:06:33.88 Intake.select :close @16:06:34.04 SSV.dial :exhaust @16:06:34.81 Exhaust.select :open @16:06:34.93 SS.seek 0ml @16:06:44.25 Exhaust.select :close @16:06:44.36 SSV.dial CBV @16:06:44.38
CBV.dial SSV @16:06:45.17 Allowing 130s for 25.0ml of remaining 500.0ml @16:06:45.18 Intake.select :open @16:06:47.34 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:06:47.36 SS.reconfigure :inhaling 1.04ml/s @16:06:47.41 SS.seek 25ml @16:07:01.88 Vacuum too high -- waiting up to 40 seconds @16:07:01.89 SS.reconfigure :exhale @16:07:01.94 SS.hold @16:07:09.51 Intake @13.6psia, vacuum from 8.1 to 11.1psi @16:07:09.53 SS.reconfigure :inhaling 0.94ml/s @16:07:09.58 SS.seek 25ml @16:07:19.41 Vacuum too high -- waiting up to 40 seconds @16:07:19.42 SS.reconfigure :exhale @16:07:19.48 SS.hold @16:07:29.47 Intake @14.2psia, vacuum from 8.7 to 11.7psi @16:07:29.48 SS.reconfigure :inhaling 0.85ml/s @16:07:29.53 SS.seek 25ml @16:07:33.12 Stroke completed -- waiting for pressure to equalize ... @16:07:33.13 SS.reconfigure :exhale @16:07:33.18 SS.hold @16:07:41.76 Intake.select :close @16:07:41.91 SSV.dial :exhaust @16:07:42.68 Exhaust.select :open @16:07:42.79 SS.seek 0ml @16:07:52.12 Exhaust.select :close @16:07:52.23 SSV.dial CBV @16:07:52.25
CBV.dial SSV @16:07:53.06 Allowing 130s for 25.0ml of remaining 475.0ml @16:07:53.07 Intake.select :open @16:07:55.23 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:07:55.25 SS.reconfigure :inhaling 0.85ml/s @16:07:55.30 SS.seek 25ml @16:08:16.10 Vacuum too high -- waiting up to 40 seconds @16:08:16.11 SS.reconfigure :exhale @16:08:16.16 SS.hold @16:08:24.84 Intake @13.7psia, vacuum from 8.2 to 11.2psi @16:08:24.86 SS.reconfigure :inhaling 0.77ml/s @16:08:24.91 SS.seek 25ml @16:08:36.48 Stroke completed -- waiting for pressure to equalize ... @16:08:36.49 SS.reconfigure :exhale @16:08:36.53 SS.hold @16:08:52.91 Intake.select :close @16:08:53.07 SSV.dial :exhaust @16:08:53.82 Exhaust.select :open @16:08:53.93 SS.seek 0ml @16:09:03.15 Exhaust.select :close @16:09:03.26 SSV.dial CBV @16:09:03.29
CBV.dial SSV @16:09:04.07 Allowing 130s for 25.0ml of remaining 450.0ml @16:09:04.08 Intake.select :open @16:09:06.24 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:09:06.26 SS.reconfigure :inhaling 0.77ml/s @16:09:06.31 SS.seek 25ml @16:09:19.75 Vacuum too high -- waiting up to 40 seconds @16:09:19.76 SS.reconfigure :exhale @16:09:19.82 SS.hold @16:09:26.43 Intake @13.7psia, vacuum from 8.2 to 11.2psi @16:09:26.45 SS.reconfigure :inhaling 0.70ml/s @16:09:26.50 SS.seek 25ml @16:09:35.69 Vacuum too high -- waiting up to 40 seconds @16:09:35.70 SS.reconfigure :exhale @16:09:36.18 SS.hold @16:09:44.84 Intake @13.9psia, vacuum from 8.4 to 11.4psi @16:09:44.86 SS.reconfigure :inhaling 0.62ml/s @16:09:44.91 SS.seek 25ml @16:09:55.70 Vacuum too high -- waiting up to 40 seconds @16:09:55.72 SS.reconfigure :exhale @16:09:55.77 SS.hold @16:10:04.48 Intake @14.0psia, vacuum from 8.5 to 11.5psi @16:10:04.50 SS.reconfigure :inhaling 0.56ml/s @16:10:04.54 SS.seek 25ml @16:10:10.97 Stroke completed -- waiting for pressure to equalize ... @16:10:10.99 SS.reconfigure :exhale @16:10:11.03 SS.hold @16:10:23.69 Intake.select :close @16:10:23.84 SSV.dial :exhaust @16:10:24.60 Exhaust.select :open @16:10:24.72 SS.seek 0ml @16:10:34.02 Exhaust.select :close @16:10:34.13 SSV.dial CBV @16:10:34.17
CBV.dial SSV @16:10:34.91 Allowing 130s for 25.0ml of remaining 425.0ml @16:10:34.92 Intake.select :open @16:10:37.08 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:10:37.10 SS.reconfigure :inhaling 0.56ml/s @16:10:37.15 SS.seek 25ml @16:10:54.31 Vacuum too high -- waiting up to 40 seconds @16:10:54.32 SS.reconfigure :exhale @16:10:54.38 SS.hold @16:11:06.05 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:11:06.07 SS.reconfigure :inhaling 0.51ml/s @16:11:06.12 SS.seek 25ml @16:11:18.81 Vacuum too high -- waiting up to 40 seconds @16:11:18.82 SS.reconfigure :exhale @16:11:18.88 SS.hold @16:11:32.08 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:11:32.10 SS.reconfigure :inhaling 0.46ml/s @16:11:32.14 SS.seek 25ml @16:11:48.59 Vacuum too high -- waiting up to 40 seconds @16:11:48.60 SS.reconfigure :exhale @16:11:48.66 SS.hold @16:12:03.21 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:12:03.23 SS.reconfigure :inhaling 0.42ml/s @16:12:03.28 SS.seek 25ml @16:12:10.10 Stroke completed -- waiting for pressure to equalize ... @16:12:10.11 SS.reconfigure :exhale @16:12:10.15 SS.hold @16:12:30.98 Intake.select :close @16:12:31.14 SSV.dial :exhaust @16:12:31.90 Exhaust.select :open @16:12:32.01 SS.seek 0ml @16:12:41.33 Exhaust.select :close @16:12:41.44 SSV.dial CBV @16:12:41.46
CBV.dial SSV @16:12:42.25 Allowing 130s for 25.0ml of remaining 400.0ml @16:12:42.27 Intake.select :open @16:12:44.43 Intake @15.2psia, vacuum from 9.7 to 12.7psi @16:12:44.45 SS.reconfigure :inhaling 0.42ml/s @16:12:44.50 SS.seek 25ml @16:13:02.25 Vacuum too high -- waiting up to 40 seconds @16:13:02.26 SS.reconfigure :exhale @16:13:02.31 SS.hold @16:13:37.21 SS.reconfigure :inhaling 0.38ml/s @16:13:37.25 SS.seek 25ml @16:13:48.18 Vacuum too high -- waiting up to 40 seconds @16:13:48.19 SS.reconfigure :exhale @16:13:48.25 SS.hold @16:14:27.26 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:14:27.27 SS.reconfigure :inhaling 0.33ml/s @16:14:27.32 SS.seek 25ml @16:14:39.06 Vacuum too high -- waiting up to 40 seconds @16:14:39.07 SS.reconfigure :exhale @16:14:39.12 SS.hold @16:14:52.27 SS.reconfigure :default @16:14:52.32 SS.hold @16:14:52.39 Intake.select :close @16:14:52.55 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 614.3ml @16:14:52.56 SSV.dial :exhaust @16:14:53.32 Exhaust.select :open @16:14:53.43 SS.reconfigure :exhale @16:14:53.48 SS.seek 0ml @16:14:59.99 Exhaust.select :close @16:15:00.10 Sampled 614.3ml @16:15:00.11 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @16:15:00.12 Intake.select :close @16:15:00.19 CSV.dial :waste @16:15:00.34 SSV.dial CBV,avoiding: :exhaust @16:15:00.37 CS.seek 0ml @16:15:00.40
CBV.dial SSV @16:15:00.54 CSV.dial :air @16:15:01.38 CS.seek 10ml @16:15:02.38
CTV.dial :air @16:15:03.16 delay 15 seconds @16:15:18.18 SSV.dial :spit,avoiding: :exhaust @16:15:19.51 CTV.dial CSV,avoiding: :intake @16:15:19.54
SS.seek 0ml @16:15:19.67 SSV.dial CBV,avoiding: :exhaust @16:15:25.32 SSV.dial between CBV and plug @16:15:25.82 CBV.dial between SSV and waste2 @16:15:26.21 CTV.dial CSV,avoiding: :air @16:15:26.23
CSV.dial CTV @16:15:26.73 CS.reconfigure :pressurize @16:15:26.77 CS.seek 0ml @16:15:50.54 BubblePoint: Intake @24.3psia, Outlet @0.1psia @16:15:50.55 CBV.dial SSV @16:15:51.05 SS.reconfigure :default @16:15:51.10 SS.seek 10ml @16:15:56.67 delay 15 seconds @16:16:11.80 Evacuated: Intake @23.7psia, Outlet @22.9psia @16:16:11.82 SSV.dial :spit,avoiding: :exhaust @16:16:12.87 SS.reconfigure :exhale @16:16:12.92 SS.seek 0ml @16:16:18.17 SS.reconfigure :fast @16:16:18.21 SS.seek 5ml @16:16:20.82 SSV.dial CBV,avoiding: :exhaust @16:16:20.84
CBV.dial SSV @16:16:22.22 CTV.dial :air,avoiding: :intake @16:16:23.60 CSV.dial CTV @16:16:23.63
SS.seek 10ml @16:16:24.77 CSV.dial :air @16:16:25.22 CS.reconfigure :fast @16:16:25.27 CS.seek 0ml @16:16:26.46
SSV.dial :spit,avoiding: :exhaust @16:16:27.80 SS.reconfigure :exhale @16:16:27.85 SS.seek 0ml @16:16:28.15 CS.stop @16:16:32.79
SS.reconfigure :fast @16:16:32.84 SS.seek 5ml @16:16:35.58 SSV.dial CBV,avoiding: :exhaust @16:16:35.62
CBV.dial SSV @16:16:37.98 SSV.dial :spit,avoiding: :exhaust @16:16:39.30 SS.reconfigure :default @16:16:39.35 SS.seek 0ml @16:16:42.76 SS.stop @16:16:42.81 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @16:16:42.82 Intake.select :close @16:16:42.88 CSV.dial :waste @16:16:43.02 SSV.dial CBV,avoiding: :exhaust @16:16:43.07
CBV.dial SSV @16:16:43.65 CS.seek 0ml @16:16:43.72 CSV.dial :air @16:16:44.41
CTV.dial :air @16:16:44.49 delay 15 seconds @16:16:44.57 CS.seek 10ml @16:16:59.50
SSV.dial :spit,avoiding: :exhaust @16:17:00.85 CTV.dial CSV,avoiding: :intake @16:17:00.88
SS.reconfigure :exhale @16:17:00.99 SS.seek 0ml @16:17:01.17 SSV.dial CBV,avoiding: :exhaust @16:17:08.44 SSV.dial between CBV and plug @16:17:08.94 CBV.dial between SSV and waste2 @16:17:09.32 CSV.dial between air and CTV @16:17:10.07 CS.seek 7.978ml @16:17:15.54 CTV.dial CSV,avoiding: :air @16:17:15.56
CSV.dial CTV @16:17:15.91 CS.reconfigure :pressurize @16:17:15.96 CS.seek 0ml @16:17:34.42 BubblePoint: Intake @24.1psia, Outlet @-0.6psia @16:17:34.43 CBV.dial SSV @16:17:34.77 SS.reconfigure :default @16:17:34.82 SS.seek 10ml @16:17:40.26 delay 15 seconds @16:17:55.40 Evacuated: Intake @22.6psia, Outlet @15.4psia @16:17:55.41 SSV.dial :spit,avoiding: :exhaust @16:17:56.46 SS.reconfigure :exhale @16:17:56.51 SS.seek 0ml @16:18:01.87 SS.reconfigure :fast @16:18:01.92 SS.seek 5ml @16:18:04.46 SSV.dial CBV,avoiding: :exhaust @16:18:04.48
CBV.dial SSV @16:18:05.86 CTV.dial :air,avoiding: :intake @16:18:07.22 CSV.dial CTV @16:18:07.26
SS.seek 10ml @16:18:08.38 CSV.dial :air @16:18:08.83 CS.reconfigure :fast @16:18:08.88 CS.seek 0ml @16:18:09.75
SSV.dial :spit,avoiding: :exhaust @16:18:11.08 SS.reconfigure :exhale @16:18:11.13 SS.seek 0ml @16:18:11.94 CS.stop @16:18:16.20
SS.reconfigure :fast @16:18:16.25 SS.seek 5ml @16:18:19.03 SSV.dial CBV,avoiding: :exhaust @16:18:19.06
CBV.dial SSV @16:18:21.43 SSV.dial :spit,avoiding: :exhaust @16:18:22.75 SS.reconfigure :default @16:18:22.81 SS.seek 0ml @16:18:26.01 SS.stop @16:18:26.05 Lysing: Adding 2.0ml AqMeOH @16:18:26.07 CBV.dial between CSV and waste1,avoiding: :DBV @16:18:27.56 CTV.dial CSV @16:18:28.91 CSV.dial CSR @16:18:29.70 Pulling 1ml of CSR.AqMeOH @16:18:29.71 CSR.select :AqMeOH @16:18:29.83 CS.reconfigure :default @16:18:29.88 CS.seek 1ml @16:18:33.22 delay 5 seconds @16:18:38.24 CSR.select :air @16:18:38.84 CS.seek 6ml @16:18:52.40 delay 2 seconds @16:18:54.42 CSV.dial :waste2 @16:18:54.86 CS.reconfigure :fast @16:18:54.91 CS.seek 0ml @16:19:09.34 CSV.dial CSR @16:19:09.79 Pulling 2ml of CSR.AqMeOH @16:19:09.80 CSR.select :AqMeOH @16:19:09.92 CS.reconfigure :default @16:19:09.97 CS.seek 2ml @16:19:15.97 delay 5 seconds @16:19:20.99 CSR.select :air @16:19:21.15 CS.seek 5ml @16:19:29.59 delay 2 seconds @16:19:31.61 CSV.dial CTV @16:19:32.05 CS.seek 1ml @16:19:43.39 Before lysing Collection Puck Heater at 17.4C/15.8psia @16:19:43.41 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @16:19:43.43 CH.seek 70C,8:00 @16:22:01.63 CH.hold 10:00,70C @16:22:01.92 Begun lysing Collection Puck Heater at 68.1C/22.2psia @16:32:01.89 Ended lysing Collection Puck Heater at 70C/20.0psia @16:32:01.90 CS.seek 0ml @16:32:05.30 CTV.dial between spare and air,avoiding: :intake @16:32:06.36 CSV.dial :air @16:32:06.81 CS.seek 5ml @16:32:20.49 CSV.dial CTV @16:32:20.94 delay 2 seconds @16:32:22.96 CSV.dial :air @16:32:23.41 delay 2 seconds @16:32:25.43 CSV.dial CTV @16:32:25.88 delay 2 seconds @16:32:27.89 CSV.dial :air @16:32:28.35 delay 2 seconds @16:32:30.37 CS.seek 0ml @16:32:44.07 Puck @27.6psia after final lysis pressurization @16:32:44.08 Recovering Lysate into Collection syringe @16:32:44.09 CSV.dial CBV @16:32:45.08 CBV.dial CSV @16:32:45.89 CS.seek 5ml @16:32:59.70 delay 5 seconds @16:33:04.72 CTV.dial :air @16:33:05.25 CS.seek 6.999ml @16:33:11.17 delay 5 seconds @16:33:16.19 CSV.dial :air2 @16:33:16.63 CS.seek 10ml @16:33:25.17 CSV.dial between air and CTV,avoiding: CBV @16:33:26.43 Lysing Complete @16:33:26.49 Storing sh1 puck in Collection Clamp to tube 1 @16:33:26.67 SC.reconfigure :tight @16:33:26.72 SC.seek 1 @16:33:30.28 SC.configure :limp @16:33:30.50 Elbow.reconfigure :tight @16:33:30.54 Elbow.seek 1 @16:33:32.29 Forearm.reconfigure :tight @16:33:32.34 Forearm.seek 1 + 800 counts @16:33:33.38 SE.reconfigure :pick @16:33:33.44 SE.seek :beamBreak @16:33:34.99 SE.configure :default @16:33:35.15 Elbow.reconfigure :limp @16:33:35.20 Forearm.reconfigure :loose @16:33:35.25 Forearm.seek 1 @16:33:35.62 Elbow.hold @16:33:35.68 Elbow.reconfigure :tight @16:33:35.78 SE.seek 15.09 Pucks below Top Plate @16:33:36.29 Forearm.force -20 @16:33:36.33 Forearm.seek 1 + 299 counts @16:33:36.69 Forearm.reconfigure :tight @16:33:36.74 Forearm.seek 1 + 800 counts @16:33:37.03 SE.seek 16.09 Pucks below Top Plate @16:33:37.72 Forearm.reconfigure :loose @16:33:37.76 Forearm.seek :retract @16:33:39.22 Elbow.seek CC @16:33:40.86 Elbow.reconfigure :loose @16:33:40.96 Forearm.seek CC @16:33:41.88 Hand.close @16:33:42.58 CC.seek :open @16:33:46.78 Forearm.seek :retract @16:33:47.89 Elbow.reconfigure :tight @16:33:47.94 Elbow.seek 1 @16:33:49.62 Elbow.reconfigure :limp @16:33:49.68 Forearm.seek 1 @16:33:50.77 Elbow.hold @16:33:50.84 Elbow.reconfigure :tight @16:33:50.89 Hand.open @16:33:51.70 Forearm.force -20 @16:33:51.75 Forearm.seek 1 + 318 counts @16:33:52.13 Forearm.reconfigure :tight @16:33:52.18 Forearm.seek 1 + 800 counts @16:33:52.52 SE.seek :down @16:33:53.66
Forearm.reconfigure :loose @16:33:53.73 Forearm.seek :retract @16:33:53.75 SE.stop @16:33:54.67
Tranferring DA @16:33:54.68 Beginning samplePassC @16:33:54.69 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:33:54.88 Elbow.seek FlushPuck::Garage @16:33:56.66 Elbow.reconfigure :loose @16:33:56.76 Forearm.seek :garage @16:33:57.81 Hand.close @16:33:59.13 Forearm.seek :retract @16:34:00.15 Elbow.reconfigure :tight @16:34:00.20 Elbow.seek CC @16:34:03.05 Elbow.reconfigure :loose @16:34:03.15 Forearm.reconfigure :tight @16:34:03.20 Forearm.seek CC @16:34:04.49 CC.seek :closed @16:34:08.50 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.05 @16:34:08.58 Hand.open @16:34:09.39 Forearm.reconfigure :loose @16:34:09.44 Forearm.seek :retract @16:34:10.40 PSV.dial PSR @16:34:11.76 Pulling 1.5ml of PSR.flush @16:34:11.77 PSR.select :flush @16:34:11.90 PS.seek 1.5ml @16:34:28.13 delay 5 seconds @16:34:33.15 PSV.dial :waste1 @16:34:33.62 PS.seek 0ml @16:34:49.94 PSV.dial PSR @16:34:50.41 PSR.select :air @16:34:50.53 PS.seek 2.5ml @16:35:16.88 PRV.dial CBV,avoiding: :ab2 @16:35:16.92
CBV.dial PRV @16:35:18.66 PSV.dial :mixloop @16:35:19.69 CTV.dial :air,avoiding: :intake @16:35:19.77 PTV.dial :PRVmixing @16:35:21.06 PS.seek 0ml @16:35:47.41 PSV.dial :air @16:35:48.31 PS.seek 2.5ml @16:36:14.63 PSV.dial :mixloop @16:36:15.09 PS.seek 0ml @16:36:41.44 PSV.dial :air @16:36:41.90 PS.seek 2.5ml @16:37:08.28 PSV.dial :mixloop @16:37:08.74 PS.seek 0ml @16:37:35.07 Garaging FlushPuck in Collection Clamp @16:37:35.14 Elbow.reconfigure :tight @16:37:35.18 Elbow.seek CC @16:37:35.49 Elbow.reconfigure :loose @16:37:35.59 Forearm.seek CC @16:37:36.62 Hand.close @16:37:37.32 CC.seek :open @16:37:41.42 Forearm.seek :retract @16:37:42.54 Elbow.reconfigure :tight @16:37:42.59 Elbow.seek :garage @16:37:45.41 Elbow.reconfigure :loose @16:37:45.51 Forearm.reconfigure :tight @16:37:45.56 Forearm.seek :garage @16:37:46.65 Hand.open @16:37:47.51 Forearm.reconfigure :loose @16:37:47.56 Forearm.seek :retract @16:37:48.55 PSV.dial :mixloop @16:37:48.56 PTV.dial :PRVmixing @16:37:48.64 PRV.dial CBV @16:37:48.67
CBV.dial PRV @16:37:48.88 CTV.dial :air,avoiding: :intake @16:37:48.96 Loading lyfil puck from tube 2 into Collection Clamp @16:37:49.20 SC.reconfigure :tight @16:37:49.24 SC.seek 2 @16:37:52.87 SC.configure :limp @16:37:53.09 Elbow.reconfigure :tight @16:37:53.56 Elbow.seek 2 @16:37:55.50 Forearm.reconfigure :tight @16:37:55.55 Forearm.seek 2 + 800 counts @16:37:56.62 SE.reconfigure :pick @16:37:56.67 SE.seek :beamBreak @16:37:59.32 SE.configure :default @16:37:59.48 Elbow.seek 2 @16:37:59.72 Forearm.seek 2 + 300 counts @16:37:59.90 Forearm.reconfigure :loose @16:37:59.94 Hand.close @16:38:00.71 SE.seek :down @16:38:00.91
Forearm.seek :retract @16:38:01.87 Elbow.seek CC @16:38:03.17 SE.stop @16:38:03.47
Elbow.reconfigure :loose @16:38:03.58 Forearm.reconfigure :tight @16:38:03.62 Forearm.seek CC @16:38:04.83 CC.seek :closed @16:38:08.81 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.01 @16:38:08.82 Hand.open @16:38:09.65 Forearm.reconfigure :loose @16:38:09.70 Forearm.seek :retract @16:38:10.65 CTV.dial CSV,avoiding: :intake @16:38:12.45 CSV.dial CTV @16:38:12.75 PS.seek 2.5ml @16:38:39.19 CS.seek 5ml @16:38:52.89 delay 8 seconds @16:39:00.91 PSV.dial :air @16:39:01.36 delay 2 seconds @16:39:03.38 PSV.dial :waste2 @16:39:04.12 PS.seek 0ml @16:39:30.47 CBV.dial between PRV and waste1 @16:39:30.80 CSV.dial :air @16:39:31.27 CS.seek 10ml @16:39:44.95 CSV.dial CTV @16:39:45.41 CS.seek 0ml @16:40:11.82 CBV.dial :waste1 @16:40:12.15 delay 5 seconds @16:40:17.17 CTV.dial :air,avoiding: :intake @16:40:18.59 Storing lyfil puck in Collection Clamp to tube 1 @16:40:18.76 SC.reconfigure :tight @16:40:18.81 SC.seek 1 @16:40:22.47 SC.configure :limp @16:40:22.69 Elbow.reconfigure :tight @16:40:22.74 Elbow.seek 1 @16:40:24.45 Forearm.reconfigure :tight @16:40:24.50 Forearm.seek 1 + 800 counts @16:40:25.61 SE.reconfigure :pick @16:40:25.67 SE.seek :beamBreak @16:40:27.19 SE.configure :default @16:40:27.35 Elbow.reconfigure :limp @16:40:27.40 Forearm.reconfigure :loose @16:40:27.45 Forearm.seek 1 @16:40:27.83 Elbow.hold @16:40:27.90 Elbow.reconfigure :tight @16:40:28.00 SE.seek 16.1 Pucks below Top Plate @16:40:28.89 Forearm.force -20 @16:40:28.94 Forearm.seek 1 + 317 counts @16:40:29.30 Forearm.reconfigure :tight @16:40:29.35 Forearm.seek 1 + 800 counts @16:40:29.58 SE.seek 17.1 Pucks below Top Plate @16:40:30.28 Forearm.reconfigure :loose @16:40:30.33 Forearm.seek :retract @16:40:31.24 Elbow.seek CC @16:40:32.88 Elbow.reconfigure :loose @16:40:32.99 Forearm.seek CC @16:40:34.00 Hand.close @16:40:34.70 CC.seek :open @16:40:38.93 Forearm.seek :retract @16:40:39.91 Elbow.reconfigure :tight @16:40:39.96 Elbow.seek 1 @16:40:41.69 Elbow.reconfigure :limp @16:40:41.74 Forearm.seek 1 @16:40:42.89 Elbow.hold @16:40:42.96 Elbow.reconfigure :tight @16:40:43.01 Hand.open @16:40:43.82 Forearm.force -20 @16:40:43.87 Forearm.seek 1 + 319 counts @16:40:44.25 Forearm.reconfigure :tight @16:40:44.30 Forearm.seek 1 + 800 counts @16:40:44.66 SE.seek :down @16:40:46.16 SE.stop @16:40:46.32
Forearm.reconfigure :loose @16:40:46.37 Forearm.seek :retract @16:40:47.27 PSV.dial :air @16:40:48.01 PS.seek 2.5ml @16:41:14.37 delay 1 second @16:41:15.39 PSV.dial :mixloop @16:41:15.85 delay 1 second @16:41:16.86 PSV.dial :air @16:41:17.33 delay 1 second @16:41:18.34 PSV.dial :mixloop @16:41:18.80 delay 1 second @16:41:19.81 PSV.dial :air @16:41:20.27 delay 1 second @16:41:21.29 PS.seek 0ml @16:41:47.63 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @16:41:47.81 Elbow.seek FlushPuck::Garage @16:41:49.59 Elbow.reconfigure :loose @16:41:49.70 Forearm.seek :garage @16:41:50.74 Hand.close @16:41:51.56 Forearm.seek :retract @16:41:52.55 Elbow.reconfigure :tight @16:41:52.60 Elbow.seek PC @16:41:53.87 Elbow.reconfigure :loose @16:41:53.98 Forearm.reconfigure :tight @16:41:54.02 Forearm.seek PC @16:41:55.40 PC.seek :closed @16:41:59.84 Processing Clamp onSpring/offSpring for 12/26 samples, on/off torque 1.55 @16:42:00.34 Hand.open @16:42:01.13 Forearm.reconfigure :loose @16:42:01.18 Forearm.seek :retract @16:42:02.28 PRV.dial :air @16:42:02.75 PSV.dial :mixloop @16:42:03.27 PS.seek 0.3ml @16:42:07.19 PSV.dial PSR @16:42:08.22 Pulling 0.4ml of PSR.air @16:42:08.23 PSR.select :air @16:42:08.25 PS.seek 0.7ml @16:42:13.12 delay 3 seconds @16:42:16.14 PSR.select :air @16:42:16.16 PS.seek 2.5ml @16:42:35.37 delay 3 seconds @16:42:38.39 PSV.dial :mixloop @16:42:39.42 PTV.dial :mixing @16:42:40.16 PBV.dial :waste2 @16:42:41.13 PS.seek 0ml @16:43:07.50 Garaging FlushPuck in Processing Clamp @16:43:07.57 Elbow.reconfigure :tight @16:43:07.62 Elbow.seek PC @16:43:07.78 Elbow.reconfigure :loose @16:43:07.88 Forearm.seek PC @16:43:08.94 Hand.close @16:43:09.70 PC.seek :open @16:43:14.18 Forearm.seek :retract @16:43:15.32 Elbow.reconfigure :tight @16:43:15.36 Elbow.seek :garage @16:43:16.73 Elbow.reconfigure :loose @16:43:16.83 Forearm.reconfigure :tight @16:43:16.88 Forearm.seek :garage @16:43:17.98 Hand.open @16:43:18.75 Forearm.reconfigure :loose @16:43:18.80 Forearm.seek :retract @16:43:19.80 Loading da puck from tube 2 into Processing Clamp @16:43:20.04 SC.reconfigure :tight @16:43:20.09 SC.seek 2 @16:43:23.70 SC.configure :limp @16:43:24.36 Elbow.reconfigure :tight @16:43:24.41 Elbow.seek 2 @16:43:26.28 Forearm.reconfigure :tight @16:43:26.33 Forearm.seek 2 + 800 counts @16:43:27.48 SE.reconfigure :pick @16:43:27.54 SE.seek :beamBreak @16:43:30.33 SE.configure :default @16:43:30.49 Elbow.seek 2 @16:43:30.68 Forearm.seek 2 + 300 counts @16:43:30.94 Forearm.reconfigure :loose @16:43:30.99 Hand.close @16:43:31.72 SE.seek :down @16:43:31.92
Forearm.seek :retract @16:43:32.97 Elbow.seek PC @16:43:34.23 SE.stop @16:43:35.54
Elbow.reconfigure :loose @16:43:35.65 Forearm.reconfigure :tight @16:43:35.69 Forearm.seek PC @16:43:37.08 PC.seek :closed @16:43:41.50 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.58 @16:43:41.52 Hand.open @16:43:42.25 Forearm.reconfigure :loose @16:43:42.30 Forearm.seek :retract @16:43:43.76 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @16:43:43.78 PH.seek 28C,5:00 @16:44:36.57 PH.hold 2:00:00,28C @16:44:36.62 delay 1:30 @16:46:06.66 PSR.select :air @16:46:06.68 PSV.dial PSR @16:46:07.72 Pulling 0.5ml of PSR.wash @16:46:07.73 PSR.select :wash @16:46:08.30 PS.seek 0.5ml @16:46:14.22 delay 5 seconds @16:46:19.23 PSR.select :air @16:46:19.36 PS.seek 2ml @16:46:35.56 PTV.dial :puck @16:46:36.29 PSV.dial PTV @16:46:37.04 PBV.dial :waste1 @16:46:38.46 PS.seek 1.5ml @16:46:44.40 delay 3 seconds @16:46:47.42 PBV.dial between PSV and waste1 @16:46:47.80 PS.seek 0ml @16:47:04.03 delay 5:00 @16:52:04.05 recoverPSRreagent08 @16:52:04.06 PTV.dial :puck @16:52:04.13 PBV.dial :waste1 @16:52:04.53 delay 30 seconds @16:52:34.55 PBV.dial between PSV and waste1 @16:52:34.93 PSR.select :air @16:52:34.95 PSV.dial PSR @16:52:35.70 PS.reconfigure :fast @16:52:35.75 PS.seek 2.5ml @16:52:59.12 PSV.dial PTV @16:52:59.88 delay 5 seconds @16:53:04.90 PSV.dial PSR @16:53:05.65 delay 5 seconds @16:53:10.67 PSV.dial PTV @16:53:11.43 delay 5 seconds @16:53:16.45 PSV.dial :air @16:53:17.22 PS.seek 0ml @16:53:40.31 PTV.dial :PRVmixing,via: PRV @16:53:41.62 PSV.dial :mixloop @16:53:42.09 PRV.dial :ab1 @16:53:42.82 PS.reconfigure :slow @16:53:42.87 PS.seek 0.15ml @16:53:46.52 delay 2 seconds @16:53:48.54 CBV.dial PRV @16:53:48.58
PRV.dial CBV @16:53:49.13 PS.seek 0.34ml @16:53:53.68 delay 2 seconds @16:53:55.70 CBV.dial between PRV and PSV @16:53:56.02 PRV.dial :air @16:53:56.48 PS.seek 0.9ml @16:54:08.43 PRV.dial :waste2 @16:54:09.17 PS.seek 0.5ml @16:54:17.84 addPMLReagent08 @16:54:17.85 PSV.dial :air @16:54:18.31 PS.reconfigure :default @16:54:18.36 PS.seek 2ml @16:54:34.49 PSV.dial :PML @16:54:34.96 PTV.dial :mixing @16:54:36.13 PBV.dial :waste1 @16:54:36.57 PS.seek 1.85ml @16:54:38.87 PBV.dial between PSV and waste1 @16:54:39.25 PS.seek 0ml @16:54:58.99 delay 2 seconds @16:55:01.01 Beginning samplePassC @16:55:01.02 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:55:01.31 Elbow.reconfigure :tight @16:55:01.36 Elbow.seek FlushPuck::Garage @16:55:02.74 Elbow.reconfigure :loose @16:55:02.84 Forearm.seek :garage @16:55:03.86 Hand.close @16:55:04.68 Forearm.seek :retract @16:55:05.74 Elbow.reconfigure :tight @16:55:05.79 Elbow.seek CC @16:55:08.56 Elbow.reconfigure :loose @16:55:08.67 Forearm.reconfigure :tight @16:55:08.71 Forearm.seek CC @16:55:10.04 CC.seek :closed @16:55:14.05 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.08 @16:55:14.13 Hand.open @16:55:14.88 Forearm.reconfigure :loose @16:55:14.93 Forearm.seek :retract @16:55:15.91 PSV.dial PSR @16:55:16.94 Pulling 1.5ml of PSR.flush @16:55:16.95 PSR.select :flush @16:55:17.53 PS.seek 1.5ml @16:55:33.71 delay 5 seconds @16:55:38.73 PSV.dial :waste1 @16:55:39.20 PS.seek 0ml @16:55:55.33 PSV.dial PSR @16:55:55.80 PSR.select :air @16:55:55.92 PS.seek 2.5ml @16:56:22.24 PRV.dial CBV,avoiding: :ab2 @16:56:22.27
CBV.dial PRV @16:56:23.90 PSV.dial :mixloop @16:56:24.93 CTV.dial :air,avoiding: :intake @16:56:25.00 PTV.dial :PRVmixing @16:56:25.75 PS.seek 0ml @16:56:52.14 PSV.dial :air @16:56:52.60 PS.seek 2.5ml @16:57:18.96 PSV.dial :mixloop @16:57:19.42 PS.seek 0ml @16:57:45.86 PSV.dial :air @16:57:46.32 PS.seek 2.5ml @16:58:12.71 PSV.dial :mixloop @16:58:13.17 PS.seek 0ml @16:58:39.74 Garaging FlushPuck in Collection Clamp @16:58:39.81 Elbow.reconfigure :tight @16:58:39.86 Elbow.seek CC @16:58:39.98 Elbow.reconfigure :loose @16:58:40.08 Forearm.seek CC @16:58:41.03 Hand.close @16:58:41.80 CC.seek :open @16:58:45.90 Forearm.seek :retract @16:58:46.97 Elbow.reconfigure :tight @16:58:47.02 Elbow.seek :garage @16:58:49.89 Elbow.reconfigure :loose @16:58:49.99 Forearm.reconfigure :tight @16:58:50.04 Forearm.seek :garage @16:58:51.13 Hand.open @16:58:51.99 Forearm.reconfigure :loose @16:58:52.03 Forearm.seek :retract @16:58:53.52 delayUntil "17:03:01.00" @16:58:53.55 Whole Cell Archive sampling 1000ml @16:58:53.57 Loading wcr puck from tube 2 into Collection Clamp @16:58:54.05 Elbow.reconfigure :tight @16:58:54.10 Elbow.seek 2 @16:58:56.00 Forearm.reconfigure :tight @16:58:56.06 Forearm.seek 2 + 800 counts @16:58:57.17 SE.reconfigure :pick @16:58:57.24 SE.seek :beamBreak @16:58:59.99 SE.configure :default @16:59:00.16 Elbow.seek 2 @16:59:00.35 Forearm.seek 2 + 300 counts @16:59:00.58 Forearm.reconfigure :loose @16:59:00.63 Hand.close @16:59:01.44 SE.seek :down @16:59:01.65 Forearm.seek :retract @16:59:02.61 Elbow.seek CC @16:59:04.17 Elbow.reconfigure :loose @16:59:04.29 SE.stop @16:59:04.45 Forearm.reconfigure :tight @16:59:04.51 Forearm.seek CC @16:59:05.78 CC.seek :closed @16:59:10.25 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.06 @16:59:10.27 Hand.open @16:59:11.11 Forearm.reconfigure :loose @16:59:11.16 Forearm.seek :retract @16:59:12.19 collectSample 1000ml w/bubble point of 35psi @16:59:12.21 Priming 7ml @16:59:12.22 Intake.select :close @16:59:12.23 Exhaust.select :close @16:59:12.24 SS.hold @16:59:12.46 Calibrating pressure sensors @16:59:12.48 CTV.dial :air @16:59:12.58 SSV.dial :spit,avoiding: :exhaust @16:59:12.66 SS.reconfigure :fast @16:59:12.72 SS.seek 5ml @16:59:15.39 SSV.dial CBV @16:59:15.43 CBV.dial SSV @16:59:16.78 delay 3.8 seconds @16:59:20.71 Sensor limits: Intake max 108.8psia; Outlet max 112.5psia @16:59:20.73 SSV.dial :spit @16:59:22.07 SS.seek 0ml @16:59:24.78 SSV.dial CBV @16:59:26.12 CTV.dial :intake @16:59:26.89 SSV.dial CBV @16:59:26.93 CBV.dial SSV @16:59:27.07 Allowing 40s for 7.0ml of remaining 7.0ml @16:59:27.08 Intake.select :open @16:59:29.25 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:59:29.27 SS.reconfigure :inhaling 2.17ml/s @16:59:29.32 SS.seek 7ml @16:59:35.76 Stroke completed -- waiting for pressure to equalize ... @16:59:35.77 SS.reconfigure :exhale @16:59:35.82 SS.hold @17:00:07.09 SS.reconfigure :default @17:00:07.14 SS.hold @17:00:07.21 Intake.select :close @17:00:07.37 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 7.0ml @17:00:07.39 SSV.dial :exhaust @17:00:08.15 Exhaust.select :open @17:00:08.66 SS.reconfigure :exhale @17:00:08.78 SS.seek 0ml @17:00:13.01 Exhaust.select :close @17:00:13.13 Sampling 1000.0ml @17:00:13.14 Intake.select :close @17:00:13.15 Exhaust.select :close @17:00:13.16 SS.reconfigure :default @17:00:13.21 SS.hold @17:00:13.43 CTV.dial :intake @17:00:13.52 SSV.dial CBV @17:00:13.54 CBV.dial SSV @17:00:14.34 Allowing 130s for 25.0ml of remaining 1000.0ml @17:00:14.35 Intake.select :open @17:00:16.52 Intake @14.4psia, vacuum from 8.9 to 11.9psi @17:00:16.54 SS.reconfigure :inhaling 2.17ml/s @17:00:16.58 SS.seek 25ml @17:00:21.88 Vacuum too high -- waiting up to 40 seconds @17:00:21.90 SS.reconfigure :exhale @17:00:21.95 SS.hold @17:01:02.60 SS.reconfigure :default @17:01:02.65 SS.hold @17:01:02.72 Intake.select :close @17:01:02.88 ShallowSampler::Clogged in WCR -- Intake @14.3psia, & Outlet @2.3psia -- no progress after 6.1ml @17:01:02.89 SSV.dial :exhaust @17:01:03.67 Exhaust.select :open @17:01:03.78 SS.reconfigure :exhale @17:01:03.83 SS.seek 0ml @17:01:07.93 Exhaust.select :close @17:01:08.04 Sampled 6.1ml @17:01:08.06 Drying puck before fixing for 4:00 @17:01:08.07 CBV.dial :waste2b @17:01:08.79 delay 1:00 @17:02:08.82 CSV.dial :air @17:02:09.28 CS.reconfigure :fast @17:02:09.34 CS.seek 10ml @17:02:33.10 Pumping wcr puck from tube 2 up to 35psi... @17:02:33.12 CBV.dial SSV @17:02:34.38 CBV.dial between CSV and waste2b @17:02:35.36 CSV.dial between air and CTV @17:02:36.14 CS.seek 7.317ml @17:02:43.06 CTV.dial CSV,avoiding: :air @17:02:43.09 CSV.dial CTV @17:02:43.91 CS.reconfigure :pressurize @17:02:43.96 CS.seek 0ml @17:03:01.01 recoverPRVreagent08 @17:03:01.02 PTV.dial :mixing @17:03:01.75 PBV.dial :waste1 @17:03:02.13 delay 30 seconds @17:03:02.74 CBV.dial :waste2b @17:03:03.09 CS.reconfigure :default @17:03:03.15 CS.seek 0ml @17:03:04.28 delay 1:00 @17:03:32.15 PBV.dial between PSV and waste1 @17:03:32.53 PSV.dial :air @17:03:32.99 PS.reconfigure :fast @17:03:33.04 PS.seek 2.5ml @17:03:56.48 PSV.dial :mixloop @17:03:56.94 delay 2 seconds @17:03:58.96 PSV.dial :air @17:03:59.41 delay 2 seconds @17:04:01.43 PSV.dial :mixloop @17:04:01.88 delay 2 seconds @17:04:03.89 PSV.dial :air @17:04:04.30 CTV.dial :air,avoiding: :intake @17:04:04.37 PS.seek 0ml @17:04:05.69 Fix #1 of 1 (with RNAlater) for 4:00 @17:04:05.70 CSV.dial CSR @17:04:06.13 Pulling 2ml of CSR.RNAlater @17:04:06.15 CSR.select :RNAlater @17:04:06.27 CS.seek 2ml @17:04:12.15 delay 5 seconds @17:04:17.17 CSR.select :air @17:04:17.29 CS.reconfigure :fast @17:04:17.34 CS.seek 10ml @17:04:27.67 washCycleDA #1 of 2 @17:04:27.68 PSR.select :air @17:04:27.70 PSV.dial PSR @17:04:29.01 Pulling 0.5ml of PSR.wash @17:04:29.03 PSR.select :wash @17:04:29.14 PS.reconfigure :default @17:04:29.19 PS.seek 0.5ml @17:04:35.11 delay 5 seconds @17:04:36.53 Pumping wcr puck from tube 2 up to 35psi... @17:04:36.55 CBV.dial SSV @17:04:37.78 CBV.dial between CSV and waste2b @17:04:38.74 CSV.dial between air and CTV @17:04:39.35 CS.seek 7.257ml @17:04:40.12 PSR.select :air @17:04:40.70 PS.seek 2ml @17:04:46.44 CTV.dial CSV,avoiding: :air @17:04:46.47 CSV.dial CTV @17:04:47.86 CS.reconfigure :pressurize @17:04:47.91 CS.seek 0ml @17:04:56.89 PTV.dial :puck @17:04:57.62 PSV.dial PTV @17:04:58.36 PBV.dial :waste1 @17:04:58.80 PS.seek 1.5ml @17:05:04.52 delay 4:00 @17:05:04.80 delay 3 seconds @17:05:07.82 PBV.dial between PSV and waste1 @17:05:08.19 PS.seek 0ml @17:05:24.30 delay 2:00 @17:07:24.33 recoverPSRreagent08 @17:07:24.34 PTV.dial :puck @17:07:24.42 PBV.dial :waste1 @17:07:24.80 delay 30 seconds @17:07:54.82 PBV.dial between PSV and waste1 @17:07:55.20 PSR.select :air @17:07:55.22 PSV.dial PSR @17:07:55.97 PS.reconfigure :fast @17:07:56.02 PS.seek 2.5ml @17:08:19.57 PSV.dial PTV @17:08:20.33 delay 5 seconds @17:08:25.35 PSV.dial PSR @17:08:26.09 delay 5 seconds @17:08:31.11 PSV.dial PTV @17:08:31.86 delay 5 seconds @17:08:36.88 PSV.dial :air @17:08:37.64 PS.seek 0ml @17:09:00.76 washCycleDA #2 of 2 @17:09:00.77 PSR.select :air @17:09:00.79 PSV.dial PSR @17:09:02.11 Pulling 0.5ml of PSR.wash @17:09:02.12 PSR.select :wash @17:09:02.24 PS.reconfigure :default @17:09:02.29 PS.seek 0.5ml @17:09:04.54 CBV.dial :waste2b @17:09:04.87 CS.reconfigure :default @17:09:04.92 CS.seek 0ml @17:09:08.11 delay 1:00 @17:09:08.20 delay 5 seconds @17:09:13.22 PSR.select :air @17:09:13.34 PS.seek 2ml @17:09:29.54 PTV.dial :puck @17:09:29.56 PSV.dial PTV @17:09:30.29 PBV.dial :waste1 @17:09:30.73 PS.seek 1.5ml @17:09:36.73 delay 3 seconds @17:09:39.75 PBV.dial between PSV and waste1 @17:09:40.13 PS.seek 0ml @17:09:56.26 delay 2:00 @17:10:08.14 CTV.dial :air,avoiding: :intake @17:11:56.35 recoverPSRreagent08 @17:11:56.36 PTV.dial :puck @17:11:56.37 PBV.dial :waste1 @17:11:56.74 delay 30 seconds @17:12:26.77 PBV.dial between PSV and waste1 @17:12:27.15 PSR.select :air @17:12:27.17 PSV.dial PSR @17:12:28.03 PS.reconfigure :fast @17:12:28.08 PS.seek 2.5ml @17:12:51.82 PSV.dial PTV @17:12:52.56 delay 5 seconds @17:12:57.58 PSV.dial PSR @17:12:58.32 delay 5 seconds @17:13:03.34 PSV.dial PTV @17:13:04.09 delay 5 seconds @17:13:09.11 PSV.dial :air @17:13:09.88 PS.seek 0ml @17:13:33.08 getPMLReagent08 @17:13:33.10 PRV.dial :air @17:13:33.54 delay 5 seconds @17:13:38.56 PTV.dial :PRVmixing @17:13:39.87 delay 5 seconds @17:13:44.89 PRV.dial :ab2 @17:13:45.34 PTV.dial :PRVmixing @17:13:45.41 PSV.dial :mixloop @17:13:45.89 PS.reconfigure :default @17:13:45.94 PS.seek 0.3ml @17:13:50.02 delay 2 seconds @17:13:52.03 PRV.dial :air @17:13:52.55 PS.seek 0.55ml @17:13:55.88 addPMLReagent08 @17:13:55.90 PSV.dial :air @17:13:56.37 PS.seek 2ml @17:14:11.97 PSV.dial :PML @17:14:12.55 PTV.dial :mixing @17:14:13.29 PBV.dial :waste1 @17:14:13.72 PS.seek 1.85ml @17:14:16.07 PBV.dial between PSV and waste1 @17:14:16.45 PS.seek 0ml @17:14:36.13 delay 2 seconds @17:14:38.15 delay 8:00 @17:22:38.18 recoverPRVreagent08 @17:22:38.19 PTV.dial :mixing @17:22:38.38 PBV.dial :waste1 @17:22:38.77 delay 30 seconds @17:23:08.80 PBV.dial between PSV and waste1 @17:23:09.19 PSV.dial :air @17:23:09.65 PS.reconfigure :fast @17:23:09.70 PS.seek 2.5ml @17:23:33.22 PSV.dial :mixloop @17:23:33.69 delay 2 seconds @17:23:35.71 PSV.dial :air @17:23:36.17 delay 2 seconds @17:23:38.19 PSV.dial :mixloop @17:23:38.66 delay 2 seconds @17:23:40.68 PSV.dial :air @17:23:41.15 PS.seek 0ml @17:24:04.47 washCycleDA #1 of 3 @17:24:04.48 PSR.select :air @17:24:04.50 PSV.dial PSR @17:24:05.83 Pulling 0.5ml of PSR.wash @17:24:05.84 PSR.select :wash @17:24:05.96 PS.reconfigure :default @17:24:06.48 PS.seek 0.5ml @17:24:12.47 delay 5 seconds @17:24:17.49 PSR.select :air @17:24:17.61 PS.seek 2ml @17:24:33.75 PTV.dial :puck @17:24:34.50 PSV.dial PTV @17:24:35.23 PBV.dial :waste1 @17:24:35.69 PS.seek 1.5ml @17:24:41.66 delay 3 seconds @17:24:44.68 PBV.dial between PSV and waste1 @17:24:45.07 PS.seek 0ml @17:25:01.16 delay 2:00 @17:27:01.19 recoverPSRreagent08 @17:27:01.20 PTV.dial :puck @17:27:01.26 PBV.dial :waste1 @17:27:01.66 delay 30 seconds @17:27:31.68 PBV.dial between PSV and waste1 @17:27:32.06 PSR.select :air @17:27:32.08 PSV.dial PSR @17:27:32.83 PS.reconfigure :fast @17:27:32.88 PS.seek 2.5ml @17:27:56.28 PSV.dial PTV @17:27:57.03 delay 5 seconds @17:28:02.05 PSV.dial PSR @17:28:02.91 delay 5 seconds @17:28:07.93 PSV.dial PTV @17:28:08.66 delay 5 seconds @17:28:13.68 PSV.dial :air @17:28:14.50 PS.seek 0ml @17:28:37.69 washCycleDA #2 of 3 @17:28:37.70 PSR.select :air @17:28:37.72 PSV.dial PSR @17:28:39.17 Pulling 0.5ml of PSR.wash @17:28:39.18 PSR.select :wash @17:28:39.30 PS.reconfigure :default @17:28:39.35 PS.seek 0.5ml @17:28:45.31 delay 5 seconds @17:28:50.33 PSR.select :air @17:28:50.45 PS.seek 2ml @17:29:06.62 PTV.dial :puck @17:29:06.64 PSV.dial PTV @17:29:07.37 PBV.dial :waste1 @17:29:07.81 PS.seek 1.5ml @17:29:13.81 delay 3 seconds @17:29:16.83 PBV.dial between PSV and waste1 @17:29:17.21 PS.seek 0ml @17:29:33.31 delay 2:00 @17:31:33.34 recoverPSRreagent08 @17:31:33.35 PTV.dial :puck @17:31:33.36 PBV.dial :waste1 @17:31:33.73 delay 30 seconds @17:32:03.76 PBV.dial between PSV and waste1 @17:32:04.13 PSR.select :air @17:32:04.15 PSV.dial PSR @17:32:04.91 PS.reconfigure :fast @17:32:04.96 PS.seek 2.5ml @17:32:28.40 PSV.dial PTV @17:32:29.15 delay 5 seconds @17:32:34.17 PSV.dial PSR @17:32:34.92 delay 5 seconds @17:32:39.94 PSV.dial PTV @17:32:40.68 delay 5 seconds @17:32:45.70 PSV.dial :air @17:32:46.95 PS.seek 0ml @17:33:10.15 washCycleDA #3 of 3 @17:33:10.16 PSR.select :air @17:33:10.18 PSV.dial PSR @17:33:11.51 Pulling 0.5ml of PSR.wash @17:33:11.52 PSR.select :wash @17:33:11.64 PS.reconfigure :default @17:33:11.69 PS.seek 0.5ml @17:33:17.56 delay 5 seconds @17:33:22.58 PSR.select :air @17:33:22.70 PS.seek 2ml @17:33:38.87 PTV.dial :puck @17:33:38.88 PSV.dial PTV @17:33:39.62 PBV.dial :waste1 @17:33:40.06 PS.seek 1.5ml @17:33:46.02 delay 3 seconds @17:33:49.04 PBV.dial between PSV and waste1 @17:33:49.42 PS.seek 0ml @17:34:05.62 delay 2:00 @17:36:05.65 recoverPSRreagent08 @17:36:05.66 PTV.dial :puck @17:36:05.67 PBV.dial :waste1 @17:36:06.04 delay 30 seconds @17:36:36.07 PBV.dial between PSV and waste1 @17:36:36.44 PSR.select :air @17:36:36.46 PSV.dial PSR @17:36:37.33 PS.reconfigure :fast @17:36:37.37 PS.seek 2.5ml @17:37:00.80 PSV.dial PTV @17:37:01.54 delay 5 seconds @17:37:06.56 PSV.dial PSR @17:37:07.43 delay 5 seconds @17:37:12.45 PSV.dial PTV @17:37:13.18 delay 5 seconds @17:37:18.20 PSV.dial :air @17:37:18.98 PS.seek 0ml @17:37:42.24 Gate.power :camera,:ON @17:37:42.26 Mixing DA Substrates @17:37:42.27 PSR.select :air @17:37:42.29 PSV.dial PSR @17:37:43.65 Pulling 0.25ml of PSR.sub1 @17:37:43.69 PSR.select :sub1 @17:37:43.89 PS.reconfigure :default @17:37:44.02 PS.seek 0.25ml @17:37:47.72 delay 5 seconds @17:37:52.74 Pulling 0.25ml of PSR.sub2 @17:37:52.75 PSR.select :sub2 @17:37:52.87 PS.seek 0.5ml @17:37:56.30 delay 5 seconds @17:38:01.32 PSR.select :air @17:38:01.44 PS.seek 2ml @17:38:17.55 PTV.dial :puck @17:38:17.57 PSV.dial PTV @17:38:18.32 PBV.dial :waste1 @17:38:18.77 PS.seek 1.5ml @17:38:24.65 delay 3 seconds @17:38:27.67 PBV.dial between PSV and waste1 @17:38:28.06 PS.seek 0ml @17:38:44.24 delay 10 seconds @17:38:54.27 recoverPSRreagent08 @17:38:54.28 PTV.dial :puck @17:38:54.29 PBV.dial :waste1 @17:38:54.68 delay 30 seconds @17:39:24.71 PBV.dial between PSV and waste1 @17:39:25.10 PSR.select :air @17:39:25.12 PSV.dial PSR @17:39:25.87 PS.reconfigure :fast @17:39:26.40 PS.seek 2.5ml @17:39:50.11 PSV.dial PTV @17:39:50.87 delay 5 seconds @17:39:55.89 PSV.dial PSR @17:39:56.63 delay 5 seconds @17:40:01.65 PSV.dial PTV @17:40:02.41 delay 5 seconds @17:40:07.43 PSV.dial :air @17:40:08.20 PS.seek 0ml @17:40:31.46 Additional air clear @17:40:31.47 PSR.select :air @17:40:31.49 PSV.dial PSR @17:40:32.82 Pulling 2ml of PSR.air @17:40:32.83 PSR.select :air @17:40:32.85 PS.reconfigure :default @17:40:32.90 PS.seek 2ml @17:40:54.11 delay 5 seconds @17:40:59.13 PTV.dial :puck @17:40:59.16 PSV.dial PTV @17:40:59.89 PBV.dial :waste1 @17:41:00.35 PS.seek 1.5ml @17:41:06.33 delay 3 seconds @17:41:09.35 PBV.dial between PSV and waste1 @17:41:09.74 delay 1 second @17:41:10.76 PSV.dial :waste1 @17:41:11.81 PS.seek 0ml @17:41:28.04 Processing Puck Heater at 27.8C before imaging @17:41:28.05 PH.coast @17:41:28.17 Imaging da puck in Processing Clamp while storing it to tube 1 @17:41:28.34 SC.reconfigure :tight @17:41:28.39 SC.seek 1 @17:41:32.02 SC.configure :limp @17:41:32.23 Elbow.reconfigure :tight @17:41:32.28 Elbow.seek 1 @17:41:34.00 Forearm.reconfigure :tight @17:41:34.05 Forearm.seek 1 + 800 counts @17:41:35.13 SE.reconfigure :pick @17:41:35.19 SE.seek :beamBreak @17:41:36.63 SE.configure :default @17:41:36.79 Elbow.reconfigure :limp @17:41:36.84 Forearm.reconfigure :loose @17:41:36.89 Forearm.seek 1 @17:41:37.30 Elbow.hold @17:41:37.37 Elbow.reconfigure :tight @17:41:37.47 SE.seek 17.1 Pucks below Top Plate @17:41:38.49 Forearm.force -20 @17:41:38.60 Forearm.seek 1 + 316 counts @17:41:39.03 Forearm.reconfigure :tight @17:41:39.08 Forearm.seek 1 + 800 counts @17:41:39.36 SE.seek 18.1 Pucks below Top Plate @17:41:39.99 Forearm.reconfigure :loose @17:41:40.04 Forearm.seek :retract @17:41:41.02 Elbow.seek PC @17:41:43.53 Elbow.reconfigure :loose @17:41:43.63 Forearm.seek PC @17:41:44.75 Hand.close @17:41:45.51 PC.seek :open @17:41:50.07 Forearm.seek :retract @17:41:51.16 Elbow.reconfigure :tight @17:41:51.21 Elbow.seek 1 @17:41:53.81 Forearm.reconfigure :tight @17:41:53.86 Forearm.seek 1 + 800 counts @17:41:54.79 Hand.open @17:41:55.58 SE.seek 17.86 Pucks below Top Plate @17:41:55.86 SE.reconfigure :seal @17:41:55.91 SE.seek 17.2 Pucks below Top Plate @17:41:56.46 Lights, camera, ACTION! @17:41:56.50 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da25oct0117h614ml.tif @17:42:15.97 Waiting >20 seconds for da to relinquish FlushPuck @17:42:16.76 SX HX9/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 16.963 seconds /var/log/esp/da25oct0117h614ml.tif: TIFF Upload Complete @17:42:16.79 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da25oct0117h614ml15s.tif @17:42:32.44 SX HX9/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da25oct0117h614ml15s.tif: TIFF Upload Complete @17:42:32.47 PuckCamera: snap -bin=2,2 21.0 /var/log/esp/midres/da25oct0117h614ml21s.tif @17:42:54.12 SX HX9/16 (USB:05): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21 seconds /var/log/esp/midres/da25oct0117h614ml21s.tif: TIFF Upload Complete @17:42:54.14 Gate.power :camera,:OFF @17:42:54.16 DAfans.email "16.963 sec auto-exposure",Subject: "da25oct0117h614ml images!" @17:42:54.58 Elbow.seek 1 @17:42:55.00 Forearm.seek 1 + 800 counts @17:42:55.14 SE.reconfigure :gently @17:42:55.21 SE.seek 17.91 Pucks below Top Plate @17:42:56.35 Elbow.reconfigure :limp @17:42:56.42 Forearm.reconfigure :loose @17:42:56.46 Forearm.seek 1 @17:42:56.86 Elbow.hold @17:42:56.93 Elbow.reconfigure :tight @17:42:56.99 SE.reconfigure :default @17:42:57.03 SE.seek 18.1 Pucks below Top Plate @17:42:57.81 Forearm.force -20 @17:42:57.96 Forearm.seek 1 + 314 counts @17:42:58.48 Forearm.reconfigure :tight @17:42:58.60 Forearm.seek 1 + 800 counts @17:42:58.97 SE.seek :down @17:42:59.97 SE.stop @17:43:00.31 Forearm.reconfigure :loose @17:43:00.41 Forearm.seek :retract @17:43:01.56 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:43:02.07 Elbow.seek FlushPuck::Garage @17:43:03.94 Elbow.reconfigure :loose @17:43:04.15 Forearm.seek :garage @17:43:05.26 Hand.close @17:43:06.27 Forearm.seek :retract @17:43:07.32 Elbow.reconfigure :tight @17:43:07.41 Elbow.seek PC @17:43:08.89 Elbow.reconfigure :loose @17:43:09.10 Forearm.reconfigure :tight @17:43:09.20 Forearm.seek PC @17:43:10.93 PC.seek :closed @17:43:15.51 Processing Clamp onSpring/offSpring for 12/27 samples, on/off torque 1.59 @17:43:15.65 Hand.open @17:43:16.53 Forearm.reconfigure :loose @17:43:16.62 Forearm.seek :retract @17:43:17.66 PSR.select :air @17:43:17.72 Storing wcr puck in Collection Clamp to tube 1 @17:43:17.76 PSV.dial PSR @17:43:18.45 Pulling 0.5ml of PSR.flush @17:43:18.49 PSR.select :flush @17:43:18.84 PS.seek 0.5ml @17:43:19.00 Elbow.reconfigure :tight @17:43:19.11 Elbow.seek 1 @17:43:21.93 Forearm.reconfigure :tight @17:43:22.05 Forearm.seek 1 + 800 counts @17:43:23.19 SE.reconfigure :pick @17:43:23.31 SE.seek :beamBreak @17:43:24.94 delay 5 seconds @17:43:25.03 SE.configure :default @17:43:25.36 Elbow.reconfigure :limp @17:43:25.46 Forearm.reconfigure :loose @17:43:25.56 Forearm.seek 1 @17:43:26.03 Elbow.hold @17:43:26.17 Elbow.reconfigure :tight @17:43:26.37 SE.seek 18.1 Pucks below Top Plate @17:43:27.13 Forearm.force -20 @17:43:27.23 Forearm.seek 1 + 297 counts @17:43:28.70 Forearm.reconfigure :tight @17:43:28.80 Forearm.seek 1 + 800 counts @17:43:29.14 SE.seek 19.1 Pucks below Top Plate @17:43:29.96 Forearm.reconfigure :loose @17:43:30.07 Forearm.seek :retract @17:43:30.17 PSR.select :air @17:43:30.35 PS.seek 2.5ml @17:43:30.99 Elbow.seek CC @17:43:31.35 upsync @17:43:32.56 Elbow.reconfigure :loose @17:43:32.67 Forearm.seek CC @17:43:33.69 Hand.close @17:43:34.38 CC.seek :open @17:43:38.61 Forearm.seek :retract @17:43:39.66 Elbow.reconfigure :tight @17:43:39.72 Elbow.seek 1 @17:43:41.45 Elbow.reconfigure :limp @17:43:41.51 Forearm.seek 1 @17:43:42.64 Elbow.hold @17:43:42.71 Elbow.reconfigure :tight @17:43:42.76 Hand.open @17:43:43.63 Forearm.force -20 @17:43:43.68 Forearm.seek 1 + 319 counts @17:43:43.97 Forearm.reconfigure :tight @17:43:44.03 Forearm.seek 1 + 800 counts @17:43:44.39 SE.seek :down @17:43:45.54 Forearm.reconfigure :loose @17:43:45.59 Forearm.seek :retract @17:43:45.72 SE.stop @17:43:46.57
Starting finalPurge @17:43:51.64 delay 1 second @17:43:52.66 PRV.dial :waste2,avoiding: :ab2 @17:43:54.53 PTV.dial :PRVmixing @17:43:55.81 PSV.dial :mixloop @17:43:56.86 PS.seek :empty @17:44:05.77
Waiting >20 seconds for psFlush to relinquish FlushPuck @17:44:23.23 PSR.select :air @17:44:23.25 PSV.dial PSR @17:44:24.30 Pulling 0.5ml of PSR.flush @17:44:24.31 PSR.select :flush @17:44:24.43 PS.seek 0.5ml @17:44:30.30 delay 5 seconds @17:44:35.32 PSR.select :air @17:44:35.44 PS.seek 2.5ml @17:44:56.74 delay 1 second @17:44:57.76 PBV.dial :waste1 @17:44:58.14 PTV.dial :mixing @17:44:58.88 PSV.dial :mixloop @17:44:59.93 PS.seek :empty @17:45:26.42 PSV.dial PSR @17:45:27.45 PSR.select :flush @17:45:27.57 PS.seek 1ml @17:45:38.67 PSR.select :air @17:45:38.79 PS.seek 2.5ml @17:45:55.20 PSV.dial PTV @17:45:55.95 PTV.dial :puck @17:45:56.69 PBV.dial :waste1 @17:45:56.77 PS.seek 0ml @17:46:23.14 PSV.dial PSR @17:46:23.87 PSR.select :flush @17:46:23.99 PS.seek 0.5ml @17:46:29.95 PSR.select :air @17:46:30.07 PS.seek 2.5ml @17:46:51.26 PSV.dial PTV @17:46:52.12 PBV.dial PSV @17:46:52.71 PS.seek 0ml @17:47:19.04 PSV.dial PBV @17:47:19.50 PS.seek 2.5ml @17:47:45.89 PSV.dial :waste1 @17:47:46.63 PS.seek 0ml @17:48:12.98 PSV.dial PSR @17:48:13.45 PSR.select :air @17:48:13.48 PS.seek 2.5ml @17:48:40.33 PSV.dial PTV @17:48:41.06 PBV.dial PSV @17:48:41.15 PS.seek 0ml @17:49:07.70 PSV.dial PBV @17:49:08.29 PS.seek 2.5ml @17:49:34.70 PSV.dial :waste1 @17:49:35.46 PS.seek 0ml @17:50:01.92 PSV.dial PSR @17:50:02.39 PSR.select :flush @17:50:02.51 PS.seek 1ml @17:50:13.57 PSR.select :air @17:50:13.69 PS.seek 2.5ml @17:50:29.92 PSV.dial PTV @17:50:30.65 PTV.dial :puck @17:50:30.73 PBV.dial :waste1 @17:50:31.32 PS.seek 0ml @17:50:57.89 PSV.dial PSR @17:50:58.64 PSR.select :flush @17:50:58.76 PS.seek 0.5ml @17:51:04.63 PSR.select :air @17:51:04.75 PS.seek 2.5ml @17:51:26.07 PSV.dial PTV @17:51:26.82 PBV.dial PSV @17:51:27.41 PS.seek 0ml @17:51:53.79 PSV.dial PBV @17:51:54.25 PS.seek 2.5ml @17:52:20.63 PSV.dial :waste1 @17:52:21.38 PS.seek 0ml @17:52:47.76 PSV.dial PSR @17:52:48.23 PSR.select :air @17:52:48.26 PS.seek 2.5ml @17:53:14.57 PSV.dial PTV @17:53:15.31 PBV.dial PSV @17:53:15.39 PS.seek 0ml @17:53:41.67 PSV.dial PBV @17:53:42.25 PS.seek 2.5ml @17:54:08.66 PSV.dial :waste1 @17:54:09.42 PS.seek 0ml @17:54:35.73
Loading FlushPuck into Collection Clamp from Processing Clamp @17:54:35.92 Elbow.seek PC @17:54:38.44 Elbow.reconfigure :loose @17:54:38.54 Forearm.seek PC @17:54:39.65 Hand.close @17:54:40.37 PC.seek :open @17:54:44.88 Forearm.seek :retract @17:54:45.89 Elbow.reconfigure :tight @17:54:45.93 Elbow.seek CC @17:54:49.47 Elbow.reconfigure :loose @17:54:49.57 Forearm.reconfigure :tight @17:54:49.62 Forearm.seek CC @17:54:50.90 CC.seek :closed @17:54:54.92 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.08 @17:54:54.99 Hand.open @17:54:55.81 Forearm.reconfigure :loose @17:54:55.86 Forearm.seek :retract @17:54:56.87 Flushing the Collection Valve::Manifold @17:54:56.99 CSV.dial CSR @17:54:57.42 Pulling 5ml of CSR.flush @17:54:57.43 CSR.select :flush @17:54:57.54 CS.seek 5ml @17:55:11.11 delay 5 seconds @17:55:16.13 CSR.select :air @17:55:16.30 CS.reconfigure :fast @17:55:16.34 CS.seek 7.5ml @17:55:22.86 CSV.dial :waste @17:55:24.13 CS.reconfigure :default @17:55:24.18 CS.seek 0ml @17:55:44.17 Flushing lower manifold's waste1 port @17:55:44.29 CSV.dial :flush @17:55:44.74 CS.reconfigure :fast @17:55:44.79 CS.seek 5ml @17:55:57.23 delay 5 seconds @17:56:02.69 CSV.dial :air @17:56:03.22 CS.seek 6.999ml @17:56:08.64 CSV.dial CTV @17:56:08.68
CTV.dial CSV @17:56:10.09 CBV.dial :waste1 @17:56:10.86 CS.seek 0ml @17:56:27.45 Flushing lower manifold's waste2 port @17:56:27.57 CSV.dial :flush @17:56:28.32 CS.seek 5ml @17:56:40.54 delay 5 seconds @17:56:45.56 CSV.dial :air @17:56:46.09 CS.seek 7ml @17:56:51.61 CSV.dial CTV @17:56:51.64
CTV.dial CSV @17:56:52.09 CBV.dial :waste2 @17:56:53.13 CS.seek 0ml @17:57:09.76 Flushing lower manifold's waste2b port @17:57:09.89 CSV.dial :flush @17:57:10.63 CS.seek 5ml @17:57:22.89 delay 5 seconds @17:57:27.91 CSV.dial :air @17:57:28.43 CS.seek 7ml @17:57:33.92 CSV.dial CTV @17:57:33.95
CTV.dial CSV @17:57:34.40 CBV.dial :waste2b @17:57:35.44 CS.seek 0ml @17:57:52.04 Disinfecting external Intake with kill @17:57:52.17 CSV.dial CSR @17:57:52.61 Pulling 8ml of CSR.kill CSR.select :kill @17:57:52.73 CS.seek 8ml @17:58:11.86 delay 5 seconds @17:58:16.87 CSR.select :air @17:58:16.99 CS.seek 10ml @17:58:22.49 CSV.dial CBV @17:58:22.52
CBV.dial CSV @17:58:23.56 CTV.dial :intake @17:58:24.34 Intake.select :open @17:58:24.45 CS.seek 2ml @17:58:43.48 Intake.select :close @17:58:43.58 CTV.dial :air @17:58:44.36 CS.reconfigure :default @17:58:44.41 CS.seek 0ml @17:58:50.38 CSV.dial CSR @17:58:51.39 Pulling 5ml of CSR.flush @17:58:51.40 CSR.select :flush @17:58:51.96 CS.seek 5ml @17:59:05.64 delay 5 seconds @17:59:10.65 CSR.select :air @17:59:10.78 CS.seek 10ml @17:59:24.38 CSV.dial CBV @17:59:25.39 CS.seek 0ml @17:59:51.79 Garaging FlushPuck in Collection Clamp @17:59:51.86 Elbow.reconfigure :tight @17:59:51.91 Elbow.seek CC @17:59:52.03 Elbow.reconfigure :loose @17:59:52.13 Forearm.seek CC @17:59:53.16 Hand.close @17:59:53.87 CC.seek :open @17:59:57.97 Forearm.seek :retract @17:59:58.94 Elbow.reconfigure :tight @17:59:58.99 Elbow.seek :garage @18:00:01.89 Elbow.reconfigure :loose @18:00:01.99 Forearm.reconfigure :tight @18:00:02.04 Forearm.seek :garage @18:00:03.15 Hand.open @18:00:03.92 Forearm.reconfigure :loose @18:00:03.97 Forearm.seek :retract @18:00:04.97 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 15:47:38 @18:00:05.03 Asleep after completing DA_WCR_100125 assays @18:00:05.04 GoodNews.email "{1 => 20,\n 2 => 2,\n 3 => 22,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@17:57:07, 39% flow, 20.8C, 18% humidity, 14.6psia, 13.74V, 0.378A avg, 72.731Ah, 5.20W\nSampled 6.1ml",Subject: "Asleep after completing DA_WCR_100125 assays" @18:00:08.79 upsync + ESPwhich DA_WCR_100825.rb + fn=DA_WCR_100825.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_100825.rb + [ -f /home/esp/esp2/mission/DA_WCR_100825.rb ] + realpath /home/esp/esp2/mission/DA_WCR_100825.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_100825.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_100825.rb @17:17:55.44UTC08-Oct-25
ESP2 v3.42 I2C Gateway v3.20 for core bus @17:17:58.13 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @17:18:01.81 Polling Can every 10:00 @17:18:01.90 Cleanly exited at 17:17:32UTC08-Oct-25 @17:18:01.96 Loading /home/esp/esp2local/mission/DA_WCR_100825.rb mission @17:18:02.01 GoodNews.email "Starting DA_WCR_100825 w/pucks in tube 2\n{1 => 20,\n 2 => 2,\n 3 => 22,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@17:18:01, 40% flow, 21.5C, 19% humidity, 14.7psia, 13.78V, 0.221A avg, 111.930Ah, 3.04W",Subject: "DA_WCR_100825 begun" @17:18:02.06
Preparing to sleep until + 00:00:05 @17:18:02.09 Asleep til + 00:00:05 @17:18:02.10 Continuing at 17:18 @17:18:02.43 Gate.power :core,:OFF @17:18:02.44 Gate.power :raw,:OFF @17:18:02.45 Shutdown @17:18:04.66 GoodNews.email "Then, in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_100825.rb:14\n{1 => 20,\n 2 => 2,\n 3 => 22,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@17:18:02, 40% flow, 21.5C, 19% humidity, 14.7psia, 13.78V, 0.227A avg, 111.930Ah, 3.13W",Subject: "Asleep until 17:18" @17:18:06.07 upsync @17:18:07.03
Powering core bus @17:18:08.58 upsync @17:18:12.50
Gate.power :raw,:ON @17:18:13.11 CS.configure :default @17:18:13.16 PS.configure :default @17:18:13.20 SS.configure :default @17:18:13.25 CC.configure :default @17:18:13.30 PC.configure :default @17:18:13.37 Forearm.configure :loose @17:18:13.42 Elbow.configure :loose @17:18:13.49 SC.configure :loose @17:18:13.53 SE.configure :default @17:18:13.58 CH.configure :default @17:18:13.62 PH.configure :default @17:18:13.66 All dwarf controllers running firmware version 3.85 @17:18:13.77 Valve.reset @17:18:13.99 Intake.select :close @17:18:14.05 Exhaust.select :close @17:18:14.09 CTV.dial :air @17:18:14.20 PSV.dial :air @17:18:14.26 CSV.dial :waste1 @17:18:14.37
Forearm.home.seek :retract @17:18:14.75 SSV.dial :spit,avoiding: :exhaust @17:18:15.15 Hand.close @17:18:15.19
Elbow.reconfigure :loose @17:18:15.28 Elbow.jog 1500 @17:18:15.35 PS.home.seek 0ml @17:18:15.61 CS.reconfigure :fast @17:18:15.64 SS.home.seek 0ml @17:18:15.72 CS.home.seek 0ml @17:18:15.80
Elbow.reconfigure :loose @17:18:15.85 Elbow.home.seek :clear @17:18:19.04 SE.home.seek :down @17:18:19.69 SC.reconfigure :tight @17:18:19.74 SC.home.seek :initial @17:18:24.53 PS.stop @17:18:48.43
SC.configure :limp @17:18:48.49 Forearm.seek :retract @17:18:48.56 Elbow.seek :hammer @17:18:49.61 Forearm.reconfigure :tight @17:18:49.66 Forearm.seek retracted @17:18:49.78 Forearm.coast @17:18:50.20 Arm::StretchError in MAIN -- Holding position retracted requires 36mA @17:18:50.21 Retry #1 of 5 @17:18:50.29 Hammering Forearm against inboard stop to recenter bearing races @17:18:50.38 Forearm.reconfigure :loose @17:18:50.43 Forearm.seek between 4 and retracted @17:18:51.04 Forearm.force 100 @17:18:51.64 Forearm.stop @17:18:52.05 Forearm.reconfigure :tight @17:18:52.10 Forearm.seek retracted @17:18:52.66 PC.home.seek :open @17:18:52.67
CC.home.seek :open @17:18:55.85 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @17:18:55.86 Notice.email "At 17:18:55 began in tube 2: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_100825.rb:14\n{1 => 20,\n 2 => 2,\n 3 => 22,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@17:18:55, 40% flow, 21.5C, 19% humidity, 14.7psia, 13.77V, 0.252A avg, 111.937Ah, 3.47W",Subject: "Started DA_WCR_100825 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @17:18:55.88
Starting initialPurge @17:18:55.91 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:18:55.97 Hand.open @17:18:56.87 Elbow.reconfigure :tight @17:18:56.93 Elbow.seek FlushPuck::Garage @17:18:58.76 Elbow.reconfigure :loose @17:18:58.88 Forearm.reconfigure :loose @17:18:58.93 Forearm.seek :garage @17:18:59.66 upsync @17:18:59.87
Hand.close @17:19:00.72 Forearm.seek :retract @17:19:01.68 Elbow.reconfigure :tight @17:19:01.73 Elbow.seek CC @17:19:04.59 Elbow.reconfigure :loose @17:19:04.69 Forearm.reconfigure :tight @17:19:04.74 Forearm.seek CC @17:19:06.09 CC.seek :closed @17:19:10.07 Collection Clamp onSpring/offSpring for 7/26 samples, on/off torque 1.99 @17:19:10.13 Hand.open @17:19:10.91 Forearm.reconfigure :loose @17:19:10.96 Forearm.seek :retract @17:19:12.03 Flushing the Collection Valve::Manifold @17:19:12.16 CSV.dial CSR @17:19:13.41 Pulling 5ml of CSR.flush @17:19:13.42 CSR.select :flush @17:19:13.54 CS.reconfigure :default @17:19:13.59 CS.seek 5ml @17:19:27.65 delay 5 seconds @17:19:32.67 CSR.select :air @17:19:32.84 CS.reconfigure :fast @17:19:32.89 CS.seek 7.5ml @17:19:39.52 CSV.dial :waste @17:19:40.79 CS.reconfigure :default @17:19:40.84 CS.seek 0ml @17:20:00.77 Purging external Intake @17:20:00.90 CSV.dial :flush @17:20:01.34 CS.reconfigure :fast @17:20:01.39 CS.seek 10ml @17:20:25.27 delay 5 seconds @17:20:30.29 CSV.dial CBV @17:20:30.33
CBV.dial CSV @17:20:31.05 CTV.dial :intake @17:20:31.82 Intake.select :open @17:20:31.93 CS.seek 0ml @17:20:55.86 Intake.select :close @17:20:55.97 CTV.dial :air @17:20:56.74 Flushing upper manifold @17:20:56.86 CSV.dial :flush @17:20:57.59 CS.seek 5ml @17:21:09.93 delay 5 seconds @17:21:14.95 CSV.dial :air @17:21:15.46 CS.seek 7ml @17:21:20.96 CSV.dial CBV @17:21:20.99
CBV.dial CSV @17:21:22.13 CTV.dial :air @17:21:22.22 CS.seek 0ml @17:21:38.93 delay 7 seconds @17:21:45.96 CS.seek 3ml @17:21:53.71 CSV.dial :waste @17:21:54.15 CS.seek 0ml @17:22:01.98 CSV.dial :flush @17:22:02.42 CS.seek 3ml @17:22:10.18 delay 5 seconds @17:22:15.20 CSV.dial :air @17:22:15.71 CS.seek 4.999ml @17:22:21.11 CSV.dial :air @17:22:21.20 CS.seek 0ml @17:22:33.46 delay 7 seconds @17:22:40.49 CS.seek 3ml @17:22:48.33 CSV.dial :waste @17:22:49.06 CS.seek 0ml @17:22:56.74 Garaging FlushPuck in Collection Clamp @17:22:56.81 Elbow.reconfigure :tight @17:22:56.85 Elbow.seek CC @17:22:57.08 Elbow.reconfigure :loose @17:22:57.18 Forearm.seek CC @17:22:58.24 Hand.close @17:22:58.96 CC.seek :open @17:23:03.15 Forearm.seek :retract @17:23:04.13 Elbow.reconfigure :tight @17:23:04.18 Elbow.seek :garage @17:23:07.01 Elbow.reconfigure :loose @17:23:07.12 Forearm.reconfigure :tight @17:23:07.16 Forearm.seek :garage @17:23:08.29 Hand.open @17:23:09.14 Forearm.reconfigure :loose @17:23:09.19 Forearm.seek :retract @17:23:10.19 da sampling at most 1000ml, wcr at most 1000ml @17:23:10.23 DAfans.email "Started da in tube 2 at 17:23:10UTC08-Oct-25\n{1 => 20,\n 2 => 2,\n 3 => 22,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@17:18:55, 40% flow, 21.5C, 19% humidity, 14.7psia, 13.77V, 0.252A avg, 111.937Ah, 3.47W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @17:23:10.27
Loading sh1 puck from tube 2 into Collection Clamp @17:23:10.53 SC.reconfigure :tight @17:23:10.58 SC.seek 2 @17:23:14.10 upsync @17:23:17.39
SC.configure :limp @17:23:17.62 Elbow.reconfigure :tight @17:23:17.68 Elbow.seek 2 @17:23:19.59 Forearm.reconfigure :tight @17:23:19.64 Forearm.seek 2 + 800 counts @17:23:20.74 SE.reconfigure :pick @17:23:20.80 SE.seek :beamBreak @17:23:23.64 SE.configure :default @17:23:23.81 Elbow.seek 2 @17:23:24.00 Forearm.seek 2 + 300 counts @17:23:24.32 Forearm.reconfigure :loose @17:23:24.36 Hand.close @17:23:25.09 SE.seek :down @17:23:25.74
Forearm.seek :retract @17:23:26.69 Elbow.seek CC @17:23:27.84 SE.stop @17:23:28.25
Elbow.reconfigure :loose @17:23:28.35 Forearm.reconfigure :tight @17:23:28.40 Forearm.seek CC @17:23:29.67 CC.seek :closed @17:23:33.65 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.03 @17:23:33.66 Hand.open @17:23:34.52 Forearm.reconfigure :loose @17:23:34.57 Forearm.seek :retract @17:23:35.56 collectSample 1000ml w/bubble point of 24psi @17:23:35.57 Priming 7ml @17:23:35.58 Intake.select :close @17:23:35.59 Exhaust.select :close @17:23:35.61 SS.hold @17:23:35.82 Calibrating pressure and temperature sensors @17:23:35.85 CTV.dial :air @17:23:35.93 SSV.dial :spit,avoiding: :exhaust @17:23:35.95 SS.reconfigure :fast @17:23:36.00 SS.seek 5ml @17:23:38.89 SSV.dial CBV @17:23:38.91
CBV.dial SSV @17:23:40.29 delay 3.8 seconds @17:23:44.22 Sensor limits: Intake max 103.6psia; Outlet max 112.5psia @17:23:44.23 SSV.dial :spit @17:23:45.58 SS.seek 0ml @17:23:48.14 SSV.dial CBV @17:23:49.48 CTV.dial :intake @17:23:50.24 SSV.dial CBV @17:23:50.28
CBV.dial SSV @17:23:50.49 Allowing 40s for 7.0ml of remaining 7.0ml @17:23:50.50 Intake.select :open @17:23:52.66 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:23:52.68 SS.reconfigure :inhaling 2.17ml/s @17:23:52.73 SS.seek 7ml @17:23:59.26 Stroke completed -- waiting for pressure to equalize ... @17:23:59.27 SS.reconfigure :exhale @17:23:59.32 SS.hold @17:24:01.52 Intake.select :close @17:24:01.67 SSV.dial :exhaust @17:24:02.45 Exhaust.select :open @17:24:02.57 SS.seek 0ml @17:24:06.52 Exhaust.select :close @17:24:06.64 Sampling 1000.0ml @17:24:06.65 Intake.select :close @17:24:06.66 Exhaust.select :close @17:24:06.67 SS.reconfigure :default @17:24:06.72 SS.hold @17:24:06.92 CTV.dial :intake @17:24:07.01 SSV.dial CBV @17:24:07.03
CBV.dial SSV @17:24:07.81 Allowing 130s for 25.0ml of remaining 1000.0ml @17:24:07.82 Intake.select :open @17:24:09.98 Intake @14.3psia, vacuum from 8.8 to 11.8psi @17:24:10.00 SS.reconfigure :inhaling 2.17ml/s @17:24:10.05 SS.seek 25ml @17:24:25.10 Stroke completed -- waiting for pressure to equalize ... @17:24:25.11 SS.reconfigure :exhale @17:24:25.16 SS.hold @17:24:31.66 Intake.select :close @17:24:31.82 SSV.dial :exhaust @17:24:32.57 Exhaust.select :open @17:24:32.68 SS.seek 0ml @17:24:41.79 Exhaust.select :close @17:24:41.90 SSV.dial CBV @17:24:41.94
CBV.dial SSV @17:24:42.68 Allowing 130s for 25.0ml of remaining 975.0ml @17:24:42.69 Intake.select :open @17:24:44.85 Intake @14.1psia, vacuum from 8.6 to 11.6psi @17:24:44.87 SS.reconfigure :inhaling 2.17ml/s @17:24:44.92 SS.seek 25ml @17:24:59.52 Stroke completed -- waiting for pressure to equalize ... @17:24:59.53 SS.reconfigure :exhale @17:24:59.58 SS.hold @17:25:06.37 Intake.select :close @17:25:06.53 SSV.dial :exhaust @17:25:07.29 Exhaust.select :open @17:25:07.40 SS.seek 0ml @17:25:16.57 Exhaust.select :close @17:25:16.68 SSV.dial CBV @17:25:16.71
CBV.dial SSV @17:25:17.48 Allowing 130s for 25.0ml of remaining 950.0ml @17:25:17.49 Intake.select :open @17:25:19.66 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:25:19.67 SS.reconfigure :inhaling 2.17ml/s @17:25:19.72 SS.seek 25ml @17:25:34.68 Stroke completed -- waiting for pressure to equalize ... @17:25:34.69 SS.reconfigure :exhale @17:25:34.73 SS.hold @17:25:41.24 Intake.select :close @17:25:41.75 SSV.dial :exhaust @17:25:42.59 Exhaust.select :open @17:25:42.70 SS.seek 0ml @17:25:51.66 Exhaust.select :close @17:25:51.77 SSV.dial CBV @17:25:51.80
CBV.dial SSV @17:25:52.57 Allowing 130s for 25.0ml of remaining 925.0ml @17:25:52.58 Intake.select :open @17:25:54.74 Intake @14.3psia, vacuum from 8.8 to 11.8psi @17:25:54.76 SS.reconfigure :inhaling 2.17ml/s @17:25:54.81 SS.seek 25ml @17:26:09.68 Stroke completed -- waiting for pressure to equalize ... @17:26:09.69 SS.reconfigure :exhale @17:26:09.73 SS.hold @17:26:16.24 Intake.select :close @17:26:16.39 SSV.dial :exhaust @17:26:17.17 Exhaust.select :open @17:26:17.28 SS.seek 0ml @17:26:26.51 Exhaust.select :close @17:26:26.62 SSV.dial CBV @17:26:26.64
CBV.dial SSV @17:26:27.41 Allowing 130s for 25.0ml of remaining 900.0ml @17:26:27.43 Intake.select :open @17:26:29.60 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:26:29.61 SS.reconfigure :inhaling 2.17ml/s @17:26:29.66 SS.seek 25ml @17:26:44.11 Stroke completed -- waiting for pressure to equalize ... @17:26:44.12 SS.reconfigure :exhale @17:26:44.17 SS.hold @17:26:51.70 Intake.select :close @17:26:51.85 SSV.dial :exhaust @17:26:52.62 Exhaust.select :open @17:26:52.73 SS.seek 0ml @17:27:01.61 Exhaust.select :close @17:27:01.72 SSV.dial CBV @17:27:01.75
CBV.dial SSV @17:27:02.52 Allowing 130s for 25.0ml of remaining 875.0ml @17:27:02.53 Intake.select :open @17:27:04.69 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:27:04.71 SS.reconfigure :inhaling 2.17ml/s @17:27:04.76 SS.seek 25ml @17:27:19.50 Stroke completed -- waiting for pressure to equalize ... @17:27:19.51 SS.reconfigure :exhale @17:27:19.56 SS.hold @17:27:28.15 Intake.select :close @17:27:28.30 SSV.dial :exhaust @17:27:29.07 Exhaust.select :open @17:27:29.19 SS.seek 0ml @17:27:38.44 Exhaust.select :close @17:27:38.55 SSV.dial CBV @17:27:38.93
CBV.dial SSV @17:27:39.77 Allowing 130s for 25.0ml of remaining 850.0ml @17:27:39.78 Intake.select :open @17:27:41.95 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:27:41.97 SS.reconfigure :inhaling 2.17ml/s @17:27:42.02 SS.seek 25ml @17:27:56.36 Stroke completed -- waiting for pressure to equalize ... @17:27:56.37 SS.reconfigure :exhale @17:27:56.42 SS.hold @17:28:04.89 Intake.select :close @17:28:05.05 SSV.dial :exhaust @17:28:05.80 Exhaust.select :open @17:28:05.91 SS.seek 0ml @17:28:15.16 Exhaust.select :close @17:28:15.27 SSV.dial CBV @17:28:15.31
CBV.dial SSV @17:28:16.05 Allowing 130s for 25.0ml of remaining 825.0ml @17:28:16.06 Intake.select :open @17:28:18.22 Intake @14.6psia, vacuum from 9.1 to 12.1psi @17:28:18.24 SS.reconfigure :inhaling 2.17ml/s @17:28:18.29 SS.seek 25ml @17:28:32.96 Stroke completed -- waiting for pressure to equalize ... @17:28:32.97 SS.reconfigure :exhale @17:28:33.01 SS.hold @17:28:43.14 Intake.select :close @17:28:43.30 SSV.dial :exhaust @17:28:44.07 Exhaust.select :open @17:28:44.18 SS.seek 0ml @17:28:53.44 Exhaust.select :close @17:28:53.55 SSV.dial CBV @17:28:53.57
CBV.dial SSV @17:28:54.35 Allowing 130s for 25.0ml of remaining 800.0ml @17:28:54.36 Intake.select :open @17:28:56.53 Intake @14.6psia, vacuum from 9.1 to 12.1psi @17:28:56.54 SS.reconfigure :inhaling 2.17ml/s @17:28:56.59 SS.seek 25ml @17:29:10.83 Stroke completed -- waiting for pressure to equalize ... @17:29:10.84 SS.reconfigure :exhale @17:29:10.89 SS.hold @17:29:22.03 Intake.select :close @17:29:22.62 SSV.dial :exhaust @17:29:23.38 Exhaust.select :open @17:29:23.49 SS.seek 0ml @17:29:32.88 Exhaust.select :close @17:29:32.99 SSV.dial CBV @17:29:33.01
CBV.dial SSV @17:29:33.80 Allowing 130s for 25.0ml of remaining 775.0ml @17:29:33.81 Intake.select :open @17:29:35.97 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:29:35.99 SS.reconfigure :inhaling 2.17ml/s @17:29:36.04 SS.seek 25ml @17:29:50.58 Stroke completed -- waiting for pressure to equalize ... @17:29:50.59 SS.reconfigure :exhale @17:29:50.64 SS.hold @17:30:01.82 Intake.select :close @17:30:01.98 SSV.dial :exhaust @17:30:02.74 Exhaust.select :open @17:30:02.85 SS.seek 0ml @17:30:12.14 Exhaust.select :close @17:30:12.25 SSV.dial CBV @17:30:12.29
CBV.dial SSV @17:30:13.04 Allowing 130s for 25.0ml of remaining 750.0ml @17:30:13.05 Intake.select :open @17:30:15.22 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:30:15.23 SS.reconfigure :inhaling 2.17ml/s @17:30:15.28 SS.seek 25ml @17:30:29.80 Stroke completed -- waiting for pressure to equalize ... @17:30:29.81 SS.reconfigure :exhale @17:30:29.85 SS.hold @17:30:42.83 Intake.select :close @17:30:42.99 SSV.dial :exhaust @17:30:43.75 Exhaust.select :open @17:30:43.86 SS.seek 0ml @17:30:53.13 Exhaust.select :close @17:30:53.24 SSV.dial CBV @17:30:53.26
CBV.dial SSV @17:30:54.03 Allowing 130s for 25.0ml of remaining 725.0ml @17:30:54.05 Intake.select :open @17:30:56.21 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:30:56.23 SS.reconfigure :inhaling 2.17ml/s @17:30:56.28 SS.seek 25ml @17:31:11.12 Stroke completed -- waiting for pressure to equalize ... @17:31:11.13 SS.reconfigure :exhale @17:31:11.18 SS.hold @17:31:25.56 Intake.select :close @17:31:25.71 SSV.dial :exhaust @17:31:26.49 Exhaust.select :open @17:31:26.60 SS.seek 0ml @17:31:35.83 Exhaust.select :close @17:31:35.94 SSV.dial CBV @17:31:35.97
CBV.dial SSV @17:31:36.75 Allowing 130s for 25.0ml of remaining 700.0ml @17:31:36.76 Intake.select :open @17:31:38.92 Intake @14.8psia, vacuum from 9.3 to 12.3psi @17:31:38.94 SS.reconfigure :inhaling 2.17ml/s @17:31:38.99 SS.seek 25ml @17:31:53.44 Stroke completed -- waiting for pressure to equalize ... @17:31:53.45 SS.reconfigure :exhale @17:31:53.49 SS.hold @17:32:09.78 Intake.select :close @17:32:09.94 SSV.dial :exhaust @17:32:10.70 Exhaust.select :open @17:32:10.82 SS.seek 0ml @17:32:20.23 Exhaust.select :close @17:32:20.34 SSV.dial CBV @17:32:20.37
CBV.dial SSV @17:32:21.14 Allowing 130s for 25.0ml of remaining 675.0ml @17:32:21.15 Intake.select :open @17:32:23.32 Intake @14.8psia, vacuum from 9.3 to 12.3psi @17:32:23.33 SS.reconfigure :inhaling 2.17ml/s @17:32:23.38 SS.seek 25ml @17:32:37.23 Vacuum too high -- waiting up to 40 seconds @17:32:37.24 SS.reconfigure :exhale @17:32:37.29 SS.hold @17:32:38.28 Intake @12.0psia, vacuum from 6.5 to 9.5psi @17:32:38.29 SS.reconfigure :inhaling 1.95ml/s @17:32:38.34 SS.seek 25ml @17:32:39.06 Stroke completed -- waiting for pressure to equalize ... @17:32:39.07 SS.reconfigure :exhale @17:32:39.12 SS.hold @17:32:53.95 Intake.select :close @17:32:54.11 SSV.dial :exhaust @17:32:54.87 Exhaust.select :open @17:32:54.99 SS.seek 0ml @17:33:03.94 Exhaust.select :close @17:33:04.05 SSV.dial CBV @17:33:04.07
CBV.dial SSV @17:33:04.84 Allowing 130s for 25.0ml of remaining 650.0ml @17:33:04.86 Intake.select :open @17:33:07.02 Intake @15.0psia, vacuum from 9.5 to 12.5psi @17:33:07.04 SS.reconfigure :inhaling 1.95ml/s @17:33:07.09 SS.seek 25ml @17:33:21.19 Vacuum too high -- waiting up to 40 seconds @17:33:21.21 SS.reconfigure :exhale @17:33:21.26 SS.hold @17:33:21.89 Intake @12.3psia, vacuum from 6.8 to 9.8psi @17:33:21.91 SS.reconfigure :inhaling 1.77ml/s @17:33:21.96 SS.seek 25ml @17:33:22.13 Vacuum too high -- waiting up to 40 seconds @17:33:22.15 SS.reconfigure :exhale @17:33:22.20 SS.hold @17:33:36.74 Intake @13.8psia, vacuum from 8.3 to 11.3psi @17:33:36.76 SS.reconfigure :inhaling 1.59ml/s @17:33:36.81 SS.seek 25ml @17:33:39.80 Stroke completed -- waiting for pressure to equalize ... @17:33:39.81 SS.reconfigure :exhale @17:33:39.86 SS.hold @17:33:43.32 Intake.select :close @17:33:43.48 SSV.dial :exhaust @17:33:44.25 Exhaust.select :open @17:33:44.36 SS.seek 0ml @17:33:53.62 Exhaust.select :close @17:33:53.73 SSV.dial CBV @17:33:53.77
CBV.dial SSV @17:33:54.53 Allowing 130s for 25.0ml of remaining 625.0ml @17:33:54.54 Intake.select :open @17:33:56.70 Intake @15.0psia, vacuum from 9.5 to 12.5psi @17:33:56.72 SS.reconfigure :inhaling 1.59ml/s @17:33:56.77 SS.seek 25ml @17:34:11.60 Vacuum too high -- waiting up to 40 seconds @17:34:11.61 SS.reconfigure :exhale @17:34:11.66 SS.hold @17:34:19.37 Intake @12.9psia, vacuum from 7.4 to 10.4psi @17:34:19.39 SS.reconfigure :inhaling 1.43ml/s @17:34:19.44 SS.seek 25ml @17:34:24.01 Stroke completed -- waiting for pressure to equalize ... @17:34:24.45 SS.reconfigure :exhale @17:34:24.50 SS.hold @17:34:34.30 Intake.select :close @17:34:34.46 SSV.dial :exhaust @17:34:35.22 Exhaust.select :open @17:34:35.33 SS.seek 0ml @17:34:44.64 Exhaust.select :close @17:34:44.75 SSV.dial CBV @17:34:44.77
CBV.dial SSV @17:34:45.56 Allowing 130s for 25.0ml of remaining 600.0ml @17:34:45.57 Intake.select :open @17:34:47.73 Intake @15.0psia, vacuum from 9.5 to 12.5psi @17:34:47.75 SS.reconfigure :inhaling 1.43ml/s @17:34:47.80 SS.seek 25ml @17:35:00.63 Vacuum too high -- waiting up to 40 seconds @17:35:00.64 SS.reconfigure :exhale @17:35:00.69 SS.hold @17:35:07.50 Intake @13.1psia, vacuum from 7.6 to 10.6psi @17:35:07.52 SS.reconfigure :inhaling 1.29ml/s @17:35:07.57 SS.seek 25ml @17:35:13.91 Vacuum too high -- waiting up to 40 seconds @17:35:13.92 SS.reconfigure :exhale @17:35:13.98 SS.hold @17:35:21.59 Intake @13.9psia, vacuum from 8.4 to 11.4psi @17:35:21.60 SS.reconfigure :inhaling 1.16ml/s @17:35:21.65 SS.seek 25ml @17:35:24.12 Stroke completed -- waiting for pressure to equalize ... @17:35:24.13 SS.reconfigure :exhale @17:35:24.18 SS.hold @17:35:29.06 Intake.select :close @17:35:29.23 SSV.dial :exhaust @17:35:30.00 Exhaust.select :open @17:35:30.11 SS.seek 0ml @17:35:39.32 Exhaust.select :close @17:35:39.44 SSV.dial CBV @17:35:39.46
CBV.dial SSV @17:35:40.23 Allowing 130s for 25.0ml of remaining 575.0ml @17:35:40.24 Intake.select :open @17:35:42.40 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:35:42.42 SS.reconfigure :inhaling 1.16ml/s @17:35:42.47 SS.seek 25ml @17:35:57.19 Vacuum too high -- waiting up to 40 seconds @17:35:57.20 SS.reconfigure :exhale @17:35:57.26 SS.hold @17:36:02.22 Intake @13.2psia, vacuum from 7.7 to 10.7psi @17:36:02.24 SS.reconfigure :inhaling 1.04ml/s @17:36:02.29 SS.seek 25ml @17:36:07.48 Vacuum too high -- waiting up to 40 seconds @17:36:07.49 SS.reconfigure :exhale @17:36:07.54 SS.hold @17:36:13.72 Intake @13.6psia, vacuum from 8.1 to 11.1psi @17:36:13.74 SS.reconfigure :inhaling 0.94ml/s @17:36:13.79 SS.seek 25ml @17:36:20.40 Stroke completed -- waiting for pressure to equalize ... @17:36:20.41 SS.reconfigure :exhale @17:36:20.46 SS.hold @17:36:27.93 Intake.select :close @17:36:28.09 SSV.dial :exhaust @17:36:28.85 Exhaust.select :open @17:36:28.97 SS.seek 0ml @17:36:38.34 Exhaust.select :close @17:36:38.45 SSV.dial CBV @17:36:38.47
CBV.dial SSV @17:36:39.26 Allowing 130s for 25.0ml of remaining 550.0ml @17:36:39.27 Intake.select :open @17:36:41.43 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:36:41.45 SS.reconfigure :inhaling 0.94ml/s @17:36:41.50 SS.seek 25ml @17:37:05.66 Vacuum too high -- waiting up to 40 seconds @17:37:05.67 SS.reconfigure :exhale @17:37:05.72 SS.hold @17:37:10.88 Intake @13.1psia, vacuum from 7.6 to 10.6psi @17:37:10.90 SS.reconfigure :inhaling 0.85ml/s @17:37:10.95 SS.seek 25ml @17:37:16.48 Stroke completed -- waiting for pressure to equalize ... @17:37:16.49 SS.reconfigure :exhale @17:37:16.53 SS.hold @17:37:29.20 Intake.select :close @17:37:29.35 SSV.dial :exhaust @17:37:30.12 Exhaust.select :open @17:37:30.23 SS.seek 0ml @17:37:39.43 Exhaust.select :close @17:37:39.54 SSV.dial CBV @17:37:39.58
CBV.dial SSV @17:37:40.32 Allowing 130s for 25.0ml of remaining 525.0ml @17:37:40.33 Intake.select :open @17:37:42.93 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:37:42.95 SS.reconfigure :inhaling 0.85ml/s @17:37:43.00 SS.seek 25ml @17:38:02.10 Vacuum too high -- waiting up to 40 seconds @17:38:02.11 SS.reconfigure :exhale @17:38:02.16 SS.hold @17:38:08.79 Intake @14.1psia, vacuum from 8.6 to 11.6psi @17:38:08.81 SS.reconfigure :inhaling 0.77ml/s @17:38:08.85 SS.seek 25ml @17:38:22.54 Stroke completed -- waiting for pressure to equalize ... @17:38:22.55 SS.reconfigure :exhale @17:38:22.60 SS.hold @17:38:35.29 Intake.select :close @17:38:35.45 SSV.dial :exhaust @17:38:36.21 Exhaust.select :open @17:38:36.32 SS.seek 0ml @17:38:45.24 Exhaust.select :close @17:38:45.35 SSV.dial CBV @17:38:45.38
CBV.dial SSV @17:38:46.15 Allowing 130s for 25.0ml of remaining 500.0ml @17:38:46.16 Intake.select :open @17:38:48.33 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:38:48.34 SS.reconfigure :inhaling 0.77ml/s @17:38:48.39 SS.seek 25ml @17:39:10.47 Vacuum too high -- waiting up to 40 seconds @17:39:10.48 SS.reconfigure :exhale @17:39:10.53 SS.hold @17:39:14.83 Intake @13.5psia, vacuum from 8.0 to 11.0psi @17:39:14.85 SS.reconfigure :inhaling 0.70ml/s @17:39:14.89 SS.seek 25ml @17:39:26.34 Vacuum too high -- waiting up to 40 seconds @17:39:26.36 SS.reconfigure :exhale @17:39:26.41 SS.hold @17:39:35.16 Intake @14.4psia, vacuum from 8.9 to 11.9psi @17:39:35.17 SS.reconfigure :inhaling 0.62ml/s @17:39:35.22 SS.seek 25ml @17:39:39.82 Stroke completed -- waiting for pressure to equalize ... @17:39:39.83 SS.reconfigure :exhale @17:39:39.88 SS.hold @17:39:47.35 Intake.select :close @17:39:47.50 SSV.dial :exhaust @17:39:48.27 Exhaust.select :open @17:39:48.38 SS.seek 0ml @17:39:57.66 Exhaust.select :close @17:39:57.77 SSV.dial CBV @17:39:57.79
CBV.dial SSV @17:39:58.59 Allowing 130s for 25.0ml of remaining 475.0ml @17:39:58.60 Intake.select :open @17:40:00.76 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:40:00.78 SS.reconfigure :inhaling 0.62ml/s @17:40:00.83 SS.seek 25ml @17:40:35.59 Vacuum too high -- waiting up to 40 seconds @17:40:35.60 SS.reconfigure :exhale @17:40:35.66 SS.hold @17:40:41.67 Intake @14.1psia, vacuum from 8.6 to 11.6psi @17:40:41.69 SS.reconfigure :inhaling 0.56ml/s @17:40:41.74 SS.seek 25ml @17:40:50.10 Stroke completed -- waiting for pressure to equalize ... @17:40:50.11 SS.reconfigure :exhale @17:40:50.15 SS.hold @17:41:03.26 Intake.select :close @17:41:03.41 SSV.dial :exhaust @17:41:04.18 Exhaust.select :open @17:41:04.29 SS.seek 0ml @17:41:13.60 Exhaust.select :close @17:41:13.71 SSV.dial CBV @17:41:13.75
CBV.dial SSV @17:41:14.52 Allowing 130s for 25.0ml of remaining 450.0ml @17:41:14.53 Intake.select :open @17:41:16.70 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:41:16.71 SS.reconfigure :inhaling 0.56ml/s @17:41:16.76 SS.seek 25ml @17:41:48.53 Vacuum too high -- waiting up to 40 seconds @17:41:48.54 SS.reconfigure :exhale @17:41:48.59 SS.hold @17:41:54.33 Intake @14.6psia, vacuum from 9.1 to 12.1psi @17:41:54.35 SS.reconfigure :inhaling 0.51ml/s @17:41:54.40 SS.seek 25ml @17:42:11.28 Stroke completed -- waiting for pressure to equalize ... @17:42:11.29 SS.reconfigure :exhale @17:42:11.34 SS.hold @17:42:24.08 Intake.select :close @17:42:24.23 SSV.dial :exhaust @17:42:25.00 Exhaust.select :open @17:42:25.12 SS.seek 0ml @17:42:34.42 Exhaust.select :close @17:42:34.53 SSV.dial CBV @17:42:34.56
CBV.dial SSV @17:42:35.32 Allowing 130s for 25.0ml of remaining 425.0ml @17:42:35.33 Intake.select :open @17:42:37.49 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:42:37.51 SS.reconfigure :inhaling 0.51ml/s @17:42:37.56 SS.seek 25ml @17:43:24.37 Vacuum too high -- waiting up to 40 seconds @17:43:24.38 SS.reconfigure :exhale @17:43:24.43 SS.hold @17:43:32.01 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:43:32.03 SS.reconfigure :inhaling 0.46ml/s @17:43:32.07 SS.seek 25ml @17:43:37.83 Stroke completed -- waiting for pressure to equalize ... @17:43:37.84 SS.reconfigure :exhale @17:43:37.90 SS.hold @17:43:49.05 Intake.select :close @17:43:49.21 SSV.dial :exhaust @17:43:49.97 Exhaust.select :open @17:43:50.08 SS.seek 0ml @17:43:59.11 Exhaust.select :close @17:43:59.22 SSV.dial CBV @17:43:59.26
CBV.dial SSV @17:44:00.00 Allowing 130s for 25.0ml of remaining 400.0ml @17:44:00.01 Intake.select :open @17:44:02.18 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:44:02.20 SS.reconfigure :inhaling 0.46ml/s @17:44:02.25 SS.seek 25ml @17:44:58.54 Stroke completed -- waiting for pressure to equalize ... @17:44:58.56 SS.reconfigure :exhale @17:44:58.60 SS.hold @17:45:18.96 Intake.select :close @17:45:19.12 SSV.dial :exhaust @17:45:19.89 Exhaust.select :open @17:45:20.06 SS.seek 0ml @17:45:29.56 Exhaust.select :close @17:45:29.67 SSV.dial CBV @17:45:29.70
CBV.dial SSV @17:45:30.49 Allowing 130s for 25.0ml of remaining 375.0ml @17:45:30.50 Intake.select :open @17:45:32.66 Intake @15.6psia, vacuum from 10.0 to 13.0psi @17:45:32.68 SS.reconfigure :inhaling 0.46ml/s @17:45:32.73 SS.seek 25ml @17:46:07.49 Vacuum too high -- waiting up to 40 seconds @17:46:07.50 SS.reconfigure :exhale @17:46:07.56 SS.hold @17:46:13.48 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:46:13.50 SS.reconfigure :inhaling 0.42ml/s @17:46:13.55 SS.seek 25ml @17:46:37.89 Stroke completed -- waiting for pressure to equalize ... @17:46:37.90 SS.reconfigure :exhale @17:46:37.95 SS.hold @17:46:54.28 Intake.select :close @17:46:54.43 SSV.dial :exhaust @17:46:55.20 Exhaust.select :open @17:46:55.31 SS.seek 0ml @17:47:04.67 Exhaust.select :close @17:47:04.78 SSV.dial CBV @17:47:04.80
CBV.dial SSV @17:47:05.59 Allowing 130s for 25.0ml of remaining 350.0ml @17:47:05.60 Intake.select :open @17:47:07.77 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:47:07.79 SS.reconfigure :inhaling 0.42ml/s @17:47:07.84 SS.seek 25ml @17:47:47.77 Vacuum too high -- waiting up to 40 seconds @17:47:47.78 SS.reconfigure :exhale @17:47:47.84 SS.hold @17:47:53.22 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:47:53.24 SS.reconfigure :inhaling 0.38ml/s @17:47:53.29 SS.seek 25ml @17:48:18.56 Stroke completed -- waiting for pressure to equalize ... @17:48:18.57 SS.reconfigure :exhale @17:48:18.61 SS.hold @17:48:34.93 Intake.select :close @17:48:35.09 SSV.dial :exhaust @17:48:35.86 Exhaust.select :open @17:48:35.97 SS.seek 0ml @17:48:45.29 Exhaust.select :close @17:48:45.40 SSV.dial CBV @17:48:45.42
CBV.dial SSV @17:48:46.20 Allowing 130s for 25.0ml of remaining 325.0ml @17:48:46.21 Intake.select :open @17:48:48.38 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:48:48.39 SS.reconfigure :inhaling 0.38ml/s @17:48:48.44 SS.seek 25ml @17:49:58.21 Stroke completed -- waiting for pressure to equalize ... @17:49:58.22 SS.reconfigure :exhale @17:49:58.27 SS.hold @17:50:18.67 Intake.select :close @17:50:18.83 SSV.dial :exhaust @17:50:19.60 Exhaust.select :open @17:50:19.71 SS.seek 0ml @17:50:29.24 Exhaust.select :close @17:50:29.35 SSV.dial CBV @17:50:29.38
CBV.dial SSV @17:50:30.17 Allowing 130s for 25.0ml of remaining 300.0ml @17:50:30.18 Intake.select :open @17:50:32.35 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:50:32.37 SS.reconfigure :inhaling 0.38ml/s @17:50:32.41 SS.seek 25ml @17:51:19.98 Vacuum too high -- waiting up to 40 seconds @17:51:20.00 SS.reconfigure :exhale @17:51:20.05 SS.hold @17:51:29.94 Intake @15.7psia, vacuum from 10.0 to 13.0psi @17:51:29.96 SS.reconfigure :inhaling 0.33ml/s @17:51:30.01 SS.seek 25ml @17:51:54.66 Stroke completed -- waiting for pressure to equalize ... @17:51:54.67 SS.reconfigure :exhale @17:51:54.72 SS.hold @17:52:19.33 Intake.select :close @17:52:19.49 SSV.dial :exhaust @17:52:20.26 Exhaust.select :open @17:52:20.37 SS.seek 0ml @17:52:29.77 Exhaust.select :close @17:52:29.88 SSV.dial CBV @17:52:29.92
CBV.dial SSV @17:52:30.66 Allowing 130s for 25.0ml of remaining 275.0ml @17:52:30.68 Intake.select :open @17:52:32.84 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:52:32.86 SS.reconfigure :inhaling 0.33ml/s @17:52:32.91 SS.seek 25ml @17:53:53.00 Stroke completed -- waiting for pressure to equalize ... @17:53:53.01 SS.reconfigure :exhale @17:53:53.06 SS.hold @17:54:19.58 Intake.select :close @17:54:19.73 SSV.dial :exhaust @17:54:20.50 Exhaust.select :open @17:54:20.61 SS.seek 0ml @17:54:30.13 Exhaust.select :close @17:54:30.24 SSV.dial CBV @17:54:30.27
CBV.dial SSV @17:54:31.05 Allowing 130s for 25.0ml of remaining 250.0ml @17:54:31.06 Intake.select :open @17:54:33.23 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:54:33.25 SS.reconfigure :inhaling 0.33ml/s @17:54:33.30 SS.seek 25ml @17:54:54.73 Vacuum too high -- waiting up to 40 seconds @17:54:54.74 SS.reconfigure :exhale @17:54:54.80 SS.hold @17:55:13.28 Intake @16.1psia, vacuum from 10.0 to 13.0psi @17:55:13.30 SS.reconfigure :inhaling 0.30ml/s @17:55:13.35 SS.seek 25ml @17:55:24.60 Vacuum too high -- waiting up to 40 seconds @17:55:24.62 SS.reconfigure :exhale @17:55:24.67 SS.hold @17:55:45.30 SS.reconfigure :inhaling 0.28ml/s @17:55:45.35 SS.seek 25ml @17:55:58.79 Vacuum too high -- waiting up to 40 seconds @17:55:58.80 SS.reconfigure :exhale @17:55:58.86 SS.hold @17:56:19.40 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:56:19.42 SS.reconfigure :inhaling 0.25ml/s @17:56:19.46 SS.seek 25ml @17:56:39.60 Vacuum too high -- waiting up to 40 seconds @17:56:39.62 SS.reconfigure :exhale @17:56:39.67 SS.hold @17:56:41.07 SS.reconfigure :default @17:56:41.12 SS.hold @17:56:41.19 Intake.select :close @17:56:41.35 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 767.4ml @17:56:41.36 SSV.dial :exhaust @17:56:42.14 Exhaust.select :open @17:56:42.26 SS.reconfigure :exhale @17:56:42.31 SS.seek 0ml @17:56:49.86 Exhaust.select :close @17:56:49.97 Sampled 767.4ml @17:56:49.99 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @17:56:50.06 CSV.dial :waste @17:56:50.20 SSV.dial CBV,avoiding: :exhaust @17:56:50.24 CS.seek 0ml @17:56:50.26
CBV.dial SSV @17:56:50.36 CSV.dial :air @17:56:51.09 CS.seek 10ml @17:56:52.19
CTV.dial :air @17:56:52.98 delay 15 seconds @17:57:08.00 SSV.dial :spit,avoiding: :exhaust @17:57:09.35 CTV.dial CSV,avoiding: :intake @17:57:09.39
SS.seek 0ml @17:57:09.51 SSV.dial CBV,avoiding: :exhaust @17:57:15.18 SSV.dial between CBV and plug @17:57:15.68 CBV.dial between SSV and waste2 @17:57:16.06 CSV.dial between air and CTV @17:57:16.37 CS.seek 8.203ml @17:57:21.31 CTV.dial CSV,avoiding: :air @17:57:21.33
CSV.dial CTV @17:57:21.68 CS.reconfigure :pressurize @17:57:21.73 CS.seek 0ml @17:57:40.27 BubblePoint: Intake @23.9psia, Outlet @0.4psia @17:57:40.28 CBV.dial SSV @17:57:40.60 SS.reconfigure :default @17:57:40.65 SS.seek 10ml @17:57:46.16 delay 15 seconds @17:58:01.29 Evacuated: Intake @23.4psia, Outlet @21.7psia @17:58:01.30 SSV.dial :spit,avoiding: :exhaust @17:58:02.35 SS.reconfigure :exhale @17:58:02.40 SS.seek 0ml @17:58:07.40 SS.reconfigure :fast @17:58:07.88 SS.seek 5ml @17:58:10.69 SSV.dial CBV,avoiding: :exhaust @17:58:10.72
CBV.dial SSV @17:58:12.10 CTV.dial :air,avoiding: :intake @17:58:13.46 CSV.dial CTV @17:58:13.51
SS.seek 10ml @17:58:14.62 CSV.dial :air @17:58:15.07 CS.reconfigure :fast @17:58:15.12 CS.seek 0ml @17:58:16.02
SSV.dial :spit,avoiding: :exhaust @17:58:17.36 SS.reconfigure :exhale @17:58:17.41 SS.seek 0ml @17:58:18.67 CS.stop @17:58:22.37
SS.reconfigure :fast @17:58:22.43 SS.seek 5ml @17:58:25.40 SSV.dial CBV,avoiding: :exhaust @17:58:25.42
CBV.dial SSV @17:58:27.79 SSV.dial :spit,avoiding: :exhaust @17:58:29.15 SS.reconfigure :default @17:58:29.19 SS.seek 0ml @17:58:32.19 SS.stop @17:58:32.23 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @17:58:32.30 CSV.dial :waste @17:58:32.43 SSV.dial CBV,avoiding: :exhaust @17:58:32.46
CBV.dial SSV @17:58:33.07 CS.seek 0ml @17:58:33.14 CSV.dial :air @17:58:33.83
CTV.dial :air @17:58:33.91 CS.seek 10ml @17:58:33.97
delay 15 seconds @17:58:48.99 SSV.dial :spit,avoiding: :exhaust @17:58:50.33 CTV.dial CSV,avoiding: :intake @17:58:50.37
SS.reconfigure :exhale @17:58:50.47 SS.seek 0ml @17:58:50.54 SSV.dial CBV,avoiding: :exhaust @17:58:57.87 SSV.dial between CBV and plug @17:58:58.36 CBV.dial between SSV and waste2 @17:58:58.77 CSV.dial between air and CTV @17:58:59.08 CS.seek 7.538ml @17:59:05.59 CTV.dial CSV,avoiding: :air @17:59:05.61
CSV.dial CTV @17:59:05.96 CS.reconfigure :pressurize @17:59:06.01 CS.seek 0ml @17:59:23.22 BubblePoint: Intake @24.0psia, Outlet @-0.2psia @17:59:23.23 CBV.dial SSV @17:59:23.57 SS.reconfigure :default @17:59:23.62 SS.seek 10ml @17:59:29.16 delay 15 seconds @17:59:44.29 Evacuated: Intake @22.8psia, Outlet @14.9psia @17:59:44.30 SSV.dial :spit,avoiding: :exhaust @17:59:45.35 SS.reconfigure :exhale @17:59:45.40 SS.seek 0ml @17:59:50.90 SS.reconfigure :fast @17:59:50.95 SS.seek 5ml @17:59:53.52 SSV.dial CBV,avoiding: :exhaust @17:59:53.54
CBV.dial SSV @17:59:54.92 CTV.dial :air,avoiding: :intake @17:59:56.29 CSV.dial CTV @17:59:56.32
SS.seek 10ml @17:59:57.45 CSV.dial :air @17:59:57.91 CS.reconfigure :fast @17:59:57.96 CS.seek 0ml @17:59:58.81
SSV.dial :spit,avoiding: :exhaust @18:00:00.15 SS.reconfigure :exhale @18:00:00.19 SS.seek 0ml @18:00:01.53 CS.stop @18:00:05.57
SS.reconfigure :fast @18:00:05.62 SS.seek 5ml @18:00:08.16 SSV.dial CBV,avoiding: :exhaust @18:00:08.20
CBV.dial SSV @18:00:10.54 SSV.dial :spit,avoiding: :exhaust @18:00:11.86 SS.reconfigure :default @18:00:11.91 SS.seek 0ml @18:00:15.12 SS.stop @18:00:15.16 Lysing: Adding 2.0ml AqMeOH @18:00:15.18 CBV.dial between CSV and waste1,avoiding: :DBV @18:00:16.68 CTV.dial CSV @18:00:18.02 CSV.dial CSR @18:00:18.81 Pulling 1ml of CSR.AqMeOH @18:00:18.82 CSR.select :AqMeOH @18:00:18.94 CS.reconfigure :default @18:00:18.99 CS.seek 1ml @18:00:22.46 delay 5 seconds @18:00:27.48 CSR.select :air @18:00:27.65 CS.seek 6ml @18:00:41.33 delay 2 seconds @18:00:43.35 CSV.dial :waste2 @18:00:43.79 CS.reconfigure :fast @18:00:43.84 CS.seek 0ml @18:00:58.36 CSV.dial CSR @18:00:58.81 Pulling 2ml of CSR.AqMeOH @18:00:58.82 CSR.select :AqMeOH @18:00:59.31 CS.reconfigure :default @18:00:59.42 CS.seek 2ml @18:01:05.33 delay 5 seconds @18:01:10.35 CSR.select :air @18:01:10.53 CS.seek 5ml @18:01:19.05 delay 2 seconds @18:01:21.07 CSV.dial CTV @18:01:21.51 CS.seek 1ml @18:01:32.76 Before lysing Collection Puck Heater at 18.7C/17.4psia @18:01:32.78 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @18:01:32.79 CH.seek 70C,8:00 @18:04:02.38 CH.hold 10:00,70C @18:04:02.58 Begun lysing Collection Puck Heater at 68.1C/23.8psia @18:14:02.59 Ended lysing Collection Puck Heater at 70C/21.3psia @18:14:02.60 CS.seek 0ml @18:14:05.92 CTV.dial between spare and air,avoiding: :intake @18:14:06.99 CSV.dial :air @18:14:07.44 CS.seek 5ml @18:14:21.04 CSV.dial CTV @18:14:21.49 delay 2 seconds @18:14:23.51 CSV.dial :air @18:14:23.96 delay 2 seconds @18:14:25.98 CSV.dial CTV @18:14:26.44 delay 2 seconds @18:14:28.45 CSV.dial :air @18:14:28.90 delay 2 seconds @18:14:30.92 CS.seek 0ml @18:14:44.65 Puck @29.2psia after final lysis pressurization @18:14:44.67 Recovering Lysate into Collection syringe @18:14:45.06 CSV.dial CBV @18:14:46.13 CBV.dial CSV @18:14:46.52 CS.seek 5ml @18:15:00.23 delay 5 seconds @18:15:05.24 CTV.dial :air @18:15:05.79 CS.seek 7ml @18:15:11.69 delay 5 seconds @18:15:16.71 CSV.dial :air2 @18:15:17.17 CS.seek 10ml @18:15:25.60 CSV.dial between air and CTV,avoiding: CBV @18:15:26.74 Lysing Complete @18:15:26.82 Storing sh1 puck in Collection Clamp to tube 1 @18:15:27.00 SC.reconfigure :tight @18:15:27.05 SC.seek 1 @18:15:30.57 SC.configure :limp @18:15:30.79 Elbow.reconfigure :tight @18:15:30.84 Elbow.seek 1 @18:15:32.54 Forearm.reconfigure :tight @18:15:32.59 Forearm.seek 1 + 800 counts @18:15:33.68 SE.reconfigure :pick @18:15:33.74 SE.seek :beamBreak @18:15:34.98 SE.configure :default @18:15:35.14 Elbow.reconfigure :limp @18:15:35.19 Forearm.reconfigure :loose @18:15:35.24 Forearm.seek 1 @18:15:37.55 Slide::Error in MAIN -- Forearm trajectoryErr at 1 @18:15:37.57 Retry #1 of 3 @18:15:37.65 Forearm.seek :retract @18:15:38.72 Elbow.reconfigure :loose @18:15:38.78 Elbow.seek 1 @18:15:38.85 Elbow.reconfigure :limp @18:15:38.91 Forearm.seek 1 @18:15:41.95 Slide::Error in MAIN -- Forearm trajectoryErr at CC @18:15:41.97 Retry #2 of 3 @18:15:42.05 Forearm.seek :retract @18:15:43.13 Elbow.reconfigure :loose @18:15:43.18 Elbow.seek 1 @18:15:43.25 Elbow.reconfigure :limp @18:15:43.32 Forearm.seek 1 @18:15:46.83 Slide::Error in MAIN -- Forearm trajectoryErr at CC @18:15:46.84 Retry #3 of 3 @18:15:46.93 Forearm.seek :retract @18:15:47.98 Elbow.reconfigure :loose @18:15:48.03 Elbow.seek 1 @18:15:48.10 Elbow.reconfigure :limp @18:15:48.17 Forearm.seek 1 @18:15:51.21 Slide::Error in MAIN -- Forearm trajectoryErr at 1 @18:15:51.22 Retry #1 of 4 @18:15:51.25 SE.seek :down @18:15:52.31 Elbow.reconfigure :tight @18:15:52.37 Elbow.seek 1 @18:15:52.57 Forearm.reconfigure :tight @18:15:52.63 Forearm.seek 1 + 800 counts @18:15:53.01 SE.reconfigure :pick @18:15:53.07 SE.seek :beamBreak @18:15:54.26 SE.configure :default @18:15:54.44 Elbow.reconfigure :limp @18:15:54.50 Forearm.reconfigure :loose @18:15:54.56 Forearm.seek 1 @18:15:56.86 Slide::Error in MAIN -- Forearm trajectoryErr at CC @18:15:56.87 Retry #1 of 3 @18:15:56.96 Forearm.seek :retract @18:15:57.98 Elbow.reconfigure :loose @18:15:58.03 Elbow.seek 1 @18:15:58.10 Elbow.reconfigure :limp @18:15:58.16 Forearm.seek 1 @18:16:01.17 Slide::Error in MAIN -- Forearm trajectoryErr at CC @18:16:01.19 Retry #2 of 3 @18:16:01.27 Forearm.seek :retract @18:16:02.29 Elbow.reconfigure :loose @18:16:02.34 Elbow.seek 1 @18:16:02.41 Elbow.reconfigure :limp @18:16:02.47 Forearm.seek 1 @18:16:05.49 Slide::Error in MAIN -- Forearm trajectoryErr at CC @18:16:05.51 Retry #3 of 3 @18:16:05.60 Forearm.seek :retract @18:16:06.61 Elbow.reconfigure :loose @18:16:06.67 Elbow.seek 1 @18:16:06.74 Elbow.reconfigure :limp @18:16:06.80 Forearm.seek 1 @18:16:10.28 Slide::Error in MAIN -- Forearm trajectoryErr at 1 @18:16:10.30 Retry #2 of 4 @18:16:10.32 SE.seek :down @18:16:11.36 Elbow.reconfigure :tight @18:16:11.41 Elbow.seek 1 @18:16:11.62 Forearm.reconfigure :tight @18:16:11.68 Forearm.seek 1 + 800 counts @18:16:12.06 SE.reconfigure :pick @18:16:12.12 SE.seek :beamBreak @18:16:13.26 SE.configure :default @18:16:13.44 Elbow.reconfigure :limp @18:16:13.51 Forearm.reconfigure :loose @18:16:13.56 Forearm.seek 1 @18:16:15.92 Slide::Error in MAIN -- Forearm trajectoryErr at 1 @18:16:15.94 Retry #1 of 3 @18:16:16.04 Forearm.seek :retract @18:16:17.10 Elbow.reconfigure :loose @18:16:17.15 Elbow.seek 1 @18:16:17.22 Elbow.reconfigure :limp @18:16:17.29 Forearm.seek 1 @18:16:20.33 Slide::Error in MAIN -- Forearm trajectoryErr at CC @18:16:20.34 Retry #2 of 3 @18:16:20.42 Forearm.seek :retract @18:16:21.48 Elbow.reconfigure :loose @18:16:21.53 Elbow.seek 1 @18:16:21.61 Elbow.reconfigure :limp @18:16:21.67 Forearm.seek 1 @18:16:24.70 Slide::Error in MAIN -- Forearm trajectoryErr at 1 @18:16:24.72 Retry #3 of 3 @18:16:24.81 Forearm.seek :retract @18:16:25.81 Elbow.reconfigure :loose @18:16:25.87 Elbow.seek 1 @18:16:25.94 Elbow.reconfigure :limp @18:16:26.01 Forearm.seek 1 @18:16:29.05 Slide::Error in MAIN -- Forearm trajectoryErr at 1 @18:16:29.07 Retry #3 of 4 @18:16:29.09 SE.seek :down @18:16:30.12 Elbow.reconfigure :tight @18:16:30.18 Elbow.seek 1 @18:16:30.84 Forearm.reconfigure :tight @18:16:30.89 Forearm.seek 1 + 800 counts @18:16:31.29 SE.reconfigure :pick @18:16:31.35 SE.seek :beamBreak @18:16:32.53 SE.configure :default @18:16:32.70 Elbow.reconfigure :limp @18:16:32.77 Forearm.reconfigure :loose @18:16:32.82 Forearm.seek 1 @18:16:35.22 Slide::Error in MAIN -- Forearm trajectoryErr at CC @18:16:35.23 Retry #1 of 3 @18:16:35.32 Forearm.seek :retract @18:16:36.41 Elbow.reconfigure :loose @18:16:36.46 Elbow.seek 1 @18:16:36.53 Elbow.reconfigure :limp @18:16:36.60 Forearm.seek 1 @18:16:39.64 Slide::Error in MAIN -- Forearm trajectoryErr at 1 @18:16:39.65 Retry #2 of 3 @18:16:39.74 Forearm.seek :retract @18:16:40.76 Elbow.reconfigure :loose @18:16:40.81 Elbow.seek 1 @18:16:40.88 Elbow.reconfigure :limp @18:16:40.94 Forearm.seek 1 @18:16:43.99 Slide::Error in MAIN -- Forearm trajectoryErr at 1 @18:16:44.00 Retry #3 of 3 @18:16:44.09 Forearm.seek :retract @18:16:45.16 Elbow.reconfigure :loose @18:16:45.21 Elbow.seek 1 @18:16:45.28 Elbow.reconfigure :limp @18:16:45.35 Forearm.seek 1 @18:16:48.39 Slide::Error in MAIN -- Forearm trajectoryErr at CC @18:16:48.41 Retry #4 of 4 @18:16:48.43 SE.seek :down @18:16:49.45 Elbow.reconfigure :tight @18:16:49.50 Elbow.seek 1 @18:16:49.71 Forearm.reconfigure :tight @18:16:49.76 Forearm.seek 1 + 800 counts @18:16:50.13 SE.reconfigure :pick @18:16:50.19 SE.seek :beamBreak @18:16:51.36 SE.configure :default @18:16:51.99 Elbow.reconfigure :limp @18:16:52.06 Forearm.reconfigure :loose @18:16:52.11 Forearm.seek 1 @18:16:54.45 Slide::Error in MAIN -- Forearm trajectoryErr at 1 @18:16:54.47 Retry #1 of 3 @18:16:54.56 Forearm.seek :retract @18:16:55.58 Elbow.reconfigure :loose @18:16:55.63 Elbow.seek 1 @18:16:55.71 Elbow.reconfigure :limp @18:16:55.77 Forearm.seek 1 @18:16:58.81 Slide::Error in MAIN -- Forearm trajectoryErr at CC @18:16:58.83 Retry #2 of 3 @18:16:58.91 Forearm.seek :retract @18:16:59.91 Elbow.reconfigure :loose @18:16:59.96 Elbow.seek 1 @18:17:00.04 Elbow.reconfigure :limp @18:17:00.10 Forearm.seek 1 @18:17:03.12 Slide::Error in MAIN -- Forearm trajectoryErr at CC @18:17:03.14 Retry #3 of 3 @18:17:03.22 Forearm.seek :retract @18:17:04.24 Elbow.reconfigure :loose @18:17:04.29 Elbow.seek 1 @18:17:04.36 Elbow.reconfigure :limp @18:17:04.43 Forearm.seek 1 @18:17:07.48 SE.seek :down @18:17:08.39 SE.stop @18:17:08.51 Forearm.seek :retract @18:17:09.50 Slide::Error in MAIN -- Forearm trajectoryErr at 1 @18:17:09.51 Waiting to Resume from Checkpoint "Forearm trajectoryErr at 1" @18:17:09.53 BadNews.email "Forearm trajectoryErr at 1",Subject: "Slide::Error in MAIN" @18:17:13.45 upsync @18:21:21.49 Session Begun @18:21:36.22 -> Arm Elbow at 1, Forearm retracted, Hand is opened @18:22:01.15 -> Flush FlushPuck is in FlushPuck::Garage @18:22:03.50 -> CC Collection Clamp contains sh1 puck from tube 2 @18:22:08.45 -> PC Processing Clamp open @18:22:32.97 -> Forearm Forearm retracted @18:22:41.48 -> Forearm.legend {-12875 => 4, -12860 => 5, -12852 => 3, -12845 => 2, -12755 => 6, -12744 => 7, -12741 => 1, -12673 => [CC,:collection,Collection], -12635 => [PC,:processing,Processing], -12550 => [:garage,FlushPuck,FlushPuck::Garage], 0 => :home, 2500 => ["retracted",:retract,:clear]} @18:23:11.23 -> SC Storage Carousel at 1 @18:23:14.83 -> SE Storage Elevator down @18:23:30.81 -> MainThread.resume @18:23:30.83
Resuming Thread after Forearm trajectoryErr at 1 at 18:17:07 # @18:23:30.88 Forearm.seek 1 @18:23:31.96 Elbow.hold @18:23:32.05 Elbow.reconfigure :tight @18:23:32.17 NoMethodError in MAIN -- undefined method `amount' for nil:NilClass @18:23:32.19 Waiting to Resume from Checkpoint "Forearm trajectoryErr at 1" @18:23:32.22 BadNews.email "undefined method `amount' for nil:NilClass",Subject: "NoMethodError in MAIN" @18:23:36.45 upsync @18:24:24.09 -> Arm.home Arm at elevator, Hand is opened @18:24:33.06 -> Arm.ready! @18:24:33.09 Forearm.reconfigure :loose @18:24:33.17 Forearm.home.seek :retract @18:24:34.74 Hand.close @18:24:34.76 Elbow.jog 1500 @18:24:35.36 Elbow.reconfigure :tight @18:24:35.44 Elbow.home.seek :clear Elbow at clear, Forearm retracted, Hand is closed @18:24:50.27 -> Thread.resume @18:24:50.30 Thread::CannotResume in dave -- No checkpoints available @18:26:01.28 -> Forearm.seek 1 @18:26:01.29 Forearm.seek 1 @18:26:02.03 Slide::Error in dave -- Elbow positionErr at clear during Forearm move @18:27:39.84 -> Arm.ready! @18:27:39.86 Forearm.reconfigure :loose @18:27:39.94 Forearm.home.seek :retract @18:27:41.16 Elbow.jog 1500 @18:27:41.70 Elbow.reconfigure :tight @18:27:41.77 Elbow.home.seek :clear Elbow at clear, Forearm retracted, Hand is closed @18:27:53.01 -> Hand.open @18:27:53.03 Hand.open Hand is opened @18:27:57.48 -> Hand.close @18:27:57.49 Hand.close Hand is closed @18:28:01.85 -> Hand.open @18:28:01.86 Hand.open Hand is opened @18:28:04.66 -> Hand.close @18:28:04.67 Hand.close Hand is closed @18:30:01.64 -> Elbow.reconfigure "limp @18:30:01.66 SyntaxError in dave -- compile error dave:19: unterminated string meets end of file @18:30:19.14 -> Forearm.seek 1 @18:30:19.15 Forearm.seek 1 @18:30:19.87 Slide::Error in dave -- Elbow positionErr at clear during Forearm move @18:31:53.41 -> Forearm.retract @18:31:53.43 Forearm.seek :retract Forearm retracted @18:32:02.11 -> Forearm.stretch! @18:32:02.13 NoMethodError in dave -- undefined method `stretch!' for Forearm:Slide @18:34:37.04 -> Arm.ready! @18:34:37.06 Forearm.reconfigure :loose @18:34:37.14 Forearm.home.seek :retract @18:34:38.04 Elbow.jog 1500 @18:34:38.67 Elbow.reconfigure :tight @18:34:38.73 Elbow.home.seek :clear Elbow at clear, Forearm retracted, Hand is closed @18:34:47.22 -> Hand.open @18:34:47.23 Hand.open Hand is opened @18:34:54.01 -> Hand.close @18:34:54.02 Hand.close Hand is closed @18:36:08.83 -> Elbow.reconfigure :tight Elbow at clear @18:36:43.25 -> Forearm Forearm retracted @18:37:02.77 -> Forearm. to 1 @18:37:02.79 Forearm.seek 1 @18:37:03.47 Slide::Error in dave -- Elbow positionErr at clear during Forearm move @18:41:19.21 -> CC.unloadPuck 1,1 @18:41:19.29 Puck::Warning in dave -- Unloading Puck.from 2, :sh1 to Storage Carousel @18:41:19.32 Storing 1 puck in Collection Clamp to tube 1 @18:41:19.33 Hand.open @18:41:20.19 Forearm.seek :retract @18:41:21.29 Elbow.seek 1 @18:41:22.48 Forearm.reconfigure :tight @18:41:22.56 Forearm.seek 1 + 800 counts @18:41:23.66 SE.reconfigure :pick @18:41:23.74 SE.seek :beamBreak @18:41:24.98 SE.configure :default @18:41:25.16 Elbow.reconfigure :limp @18:41:25.24 Forearm.reconfigure :loose @18:41:25.30 Forearm.seek 1 @18:41:27.69 Slide::Error in dave -- Forearm trajectoryErr at 1 @18:41:27.71 Retry #1 of 3 @18:41:27.83 Forearm.seek :retract @18:41:28.87 Elbow.reconfigure :loose @18:41:28.95 Elbow.seek 1 @18:41:29.04 Elbow.reconfigure :limp @18:41:29.12 Forearm.seek 1 @18:41:32.18 Slide::Error in dave -- Forearm trajectoryErr at 1 @18:41:32.20 Retry #2 of 3 @18:41:32.32 Forearm.seek :retract @18:41:33.35 Elbow.reconfigure :loose @18:41:33.43 Elbow.seek 1 @18:41:33.51 Elbow.reconfigure :limp @18:41:33.59 Forearm.seek 1 @18:41:36.63 Slide::Error in dave -- Forearm trajectoryErr at 1 @18:41:36.65 Retry #3 of 3 @18:41:36.76 Forearm.seek :retract @18:41:37.81 Elbow.reconfigure :loose @18:41:37.89 Elbow.seek 1 @18:41:37.98 Elbow.reconfigure :limp @18:41:38.06 Forearm.seek 1 @18:41:41.09 Slide::Error in dave -- Forearm trajectoryErr at 1 @18:41:41.11 Retry #1 of 4 @18:41:41.14 SE.seek :down @18:41:42.24 Elbow.reconfigure :tight @18:41:42.31 Elbow.seek 1 @18:41:43.06 Forearm.reconfigure :tight @18:41:43.14 Forearm.seek 1 + 800 counts @18:41:43.52 SE.reconfigure :pick @18:41:43.61 SE.seek :beamBreak @18:41:44.78 SE.configure :default @18:41:44.98 Elbow.reconfigure :limp @18:41:45.06 Forearm.reconfigure :loose @18:41:45.13 Forearm.seek 1 @18:41:47.52 Slide::Error in dave -- Forearm trajectoryErr at 1 @18:41:47.55 Retry #1 of 3 @18:41:47.66 Forearm.seek :retract @18:41:48.71 Elbow.reconfigure :loose @18:41:48.79 Elbow.seek 1 @18:41:48.88 Elbow.reconfigure :limp @18:41:48.96 Forearm.seek 1 @18:41:52.01 Slide::Error in dave -- Forearm trajectoryErr at CC @18:41:52.02 Retry #2 of 3 @18:41:52.14 Forearm.seek :retract @18:41:53.38 Elbow.reconfigure :loose @18:41:53.45 Elbow.seek 1 @18:41:53.55 Elbow.reconfigure :limp @18:41:53.63 Forearm.seek 1 @18:41:56.71 Slide::Error in dave -- Forearm trajectoryErr at 1 @18:41:56.73 Retry #3 of 3 @18:41:56.85 Forearm.seek :retract @18:41:57.88 Elbow.reconfigure :loose @18:41:57.96 Elbow.seek 1 @18:41:58.05 Elbow.reconfigure :limp @18:41:58.13 Forearm.seek 1 @18:42:01.20 Slide::Error in dave -- Forearm trajectoryErr at 1 @18:42:01.22 Retry #2 of 4 @18:42:01.26 SE.seek :down @18:42:02.31 Elbow.reconfigure :tight @18:42:02.39 Elbow.seek 1 @18:42:02.66 Forearm.reconfigure :tight @18:42:02.74 Forearm.seek 1 + 800 counts @18:42:03.13 SE.reconfigure :pick @18:42:03.21 SE.seek :beamBreak @18:42:04.39 SE.configure :default @18:42:04.58 Elbow.reconfigure :limp @18:42:04.66 Forearm.reconfigure :loose @18:42:04.73 Forearm.seek 1 @18:42:07.08 Slide::Error in dave -- Forearm trajectoryErr at CC @18:42:07.11 Retry #1 of 3 @18:42:07.21 Forearm.seek :retract @18:42:08.77 Elbow.reconfigure :loose @18:42:08.86 Elbow.seek 1 @18:42:08.95 Elbow.reconfigure :limp @18:42:09.03 Forearm.seek 1 @18:42:12.10 Slide::Error in dave -- Forearm trajectoryErr at 1 @18:42:12.12 Retry #2 of 3 @18:42:12.24 Forearm.seek :retract @18:42:13.27 Elbow.reconfigure :loose @18:42:13.35 Elbow.seek 1 @18:42:13.44 Elbow.reconfigure :limp @18:42:13.52 Forearm.seek 1 @18:42:16.61 Slide::Error in dave -- Forearm trajectoryErr at CC @18:42:16.63 Retry #3 of 3 @18:42:16.75 Forearm.seek :retract @18:42:17.84 Elbow.reconfigure :loose @18:42:17.92 Elbow.seek 1 @18:42:18.01 Elbow.reconfigure :limp @18:42:18.09 Forearm.seek 1 @18:42:21.17 Slide::Error in dave -- Forearm trajectoryErr at 1 @18:42:21.19 Retry #3 of 4 @18:42:21.23 SE.seek :down @18:42:22.29 Elbow.reconfigure :tight @18:42:22.37 Elbow.seek 1 @18:42:22.65 Forearm.reconfigure :tight @18:42:22.73 Forearm.seek 1 + 800 counts @18:42:23.12 SE.reconfigure :pick @18:42:23.20 SE.seek :beamBreak @18:42:24.42 SE.configure :default @18:42:24.61 Elbow.reconfigure :limp @18:42:24.70 Forearm.reconfigure :loose @18:42:24.77 Forearm.seek 1 @18:42:27.19 Slide::Error in dave -- Forearm trajectoryErr at CC @18:42:27.21 Retry #1 of 3 @18:42:27.32 Forearm.seek :retract @18:42:28.49 Elbow.reconfigure :loose @18:42:28.57 Elbow.seek 1 @18:42:28.65 Elbow.reconfigure :limp @18:42:28.73 Forearm.seek 1 @18:42:31.75 Slide::Error in dave -- Forearm trajectoryErr at 1 @18:42:31.77 Retry #2 of 3 @18:42:31.88 Forearm.seek :retract @18:42:32.92 Elbow.reconfigure :loose @18:42:33.00 Elbow.seek 1 @18:42:33.08 Elbow.reconfigure :limp @18:42:33.16 Forearm.seek 1 @18:42:36.22 Slide::Error in dave -- Forearm trajectoryErr at CC @18:42:36.24 Retry #3 of 3 @18:42:36.93 Forearm.seek :retract @18:42:37.99 Elbow.reconfigure :loose @18:42:38.06 Elbow.seek 1 @18:42:38.16 Elbow.reconfigure :limp @18:42:38.24 Forearm.seek 1 @18:42:41.31 Slide::Error in dave -- Forearm trajectoryErr at 1 @18:42:41.33 Retry #4 of 4 @18:42:41.36 SE.seek :down @18:42:42.40 Elbow.reconfigure :tight @18:42:42.48 Elbow.seek 1 @18:42:42.70 Forearm.reconfigure :tight @18:42:42.78 Forearm.seek 1 + 800 counts @18:42:43.16 SE.reconfigure :pick @18:42:43.25 SE.seek :beamBreak @18:42:44.46 SE.configure :default @18:42:44.64 Elbow.reconfigure :limp @18:42:44.73 Forearm.reconfigure :loose @18:42:44.79 Forearm.seek 1 @18:42:47.21 Slide::Error in dave -- Forearm trajectoryErr at CC @18:42:47.23 Retry #1 of 3 @18:42:47.34 Forearm.seek :retract @18:42:48.47 Elbow.reconfigure :loose @18:42:48.54 Elbow.seek 1 @18:42:48.63 Elbow.reconfigure :limp @18:42:48.72 Forearm.seek 1 @18:42:51.77 Slide::Error in dave -- Forearm trajectoryErr at CC @18:42:51.79 Retry #2 of 3 @18:42:51.90 Forearm.seek :retract @18:42:52.96 Elbow.reconfigure :loose @18:42:53.03 Elbow.seek 1 @18:42:53.13 Elbow.reconfigure :limp @18:42:53.21 Forearm.seek 1 @18:42:56.27 Slide::Error in dave -- Forearm trajectoryErr at 1 @18:42:56.29 Retry #3 of 3 @18:42:56.40 Forearm.seek :retract @18:42:57.42 Elbow.reconfigure :loose @18:42:57.50 Elbow.seek 1 @18:42:57.58 Elbow.reconfigure :limp @18:42:57.66 Forearm.seek 1 @18:43:00.75 SE.seek :down @18:43:01.79 SE.stop @18:43:01.92 Forearm.seek :retract @18:43:02.89 Slide::Error in dave -- Forearm trajectoryErr at 1 @18:44:01.98 -> CC Collection Clamp contains sh1 puck from tube 2 @18:44:07.38 -> Arm.ready! @18:44:07.41 Forearm.reconfigure :loose @18:44:07.48 Forearm.home.seek :retract @18:44:08.29 Hand.close @18:44:08.92 Elbow.jog 1500 @18:44:12.08 Elbow.reconfigure :limp @18:44:12.14 Elbow.home.seek :clear @18:44:15.25 Slide::Error in dave -- Elbow trajectoryErr at 4 @18:44:42.71 -> Arm.ready! @18:44:42.73 Forearm.reconfigure :loose @18:44:42.80 Forearm.home.seek :retract @18:44:43.58 Elbow.reconfigure :limp @18:44:43.65 Elbow.jog 1500 @18:44:46.75 Elbow.reconfigure :limp @18:44:46.82 Elbow.home.seek :clear @18:44:49.94 Slide::Error in dave -- Elbow trajectoryErr at 4 @18:46:30.08 -> Forearm Forearm retracted @18:46:32.55 -> Arm Elbow at 4, Forearm retracted, Hand is closed @18:47:05.03 -> Thread.recover @18:47:05.04 Resuming after Elbow trajectoryErr at 4 at 18:44:49 @18:47:05.07 Elbow.reconfigure :limp @18:47:05.15 Elbow.seek :clear @18:47:08.27 Slide::Error in dave -- Elbow trajectoryErr at 4 @18:48:28.07 -> Arm Elbow at 4, Forearm retracted, Hand is closed @18:48:34.06 -> Arm.pick @18:48:34.22 Elbow.reconfigure :tight @18:48:34.29 Elbow.seek 1 @18:48:34.50 Forearm.reconfigure :tight @18:48:34.57 Forearm.seek 1 + 800 counts Elbow at 4, Forearm at garage + 568 counts, Hand is closed @18:48:50.30 -> Arm.retract @18:48:50.32 Hand.open @18:48:51.18 Forearm.reconfigure :loose @18:48:51.24 Forearm.seek :retract Elbow at 4, Forearm retracted, Hand is opened @18:49:10.04 -> Arm.pick @18:49:10.19 Elbow.seek 1 @18:49:10.39 Forearm.reconfigure :tight @18:49:10.46 Forearm.seek 1 + 800 counts Elbow at 4, Forearm at garage + 590 counts, Hand is opened @18:49:47.05 -> Arm.ready! @18:49:47.08 Forearm.reconfigure :loose @18:49:47.15 Forearm.home.seek :retract @18:49:48.60 Hand.close @18:49:48.65 Elbow.jog 1500 @18:49:49.20 Elbow.reconfigure :tight @18:49:49.27 Elbow.home.seek :clear Elbow at clear, Forearm retracted, Hand is closed @18:50:04.40 -> Arm.pick @18:50:04.61 Elbow.seek 1 @18:50:05.72 Forearm.reconfigure :tight @18:50:05.79 Forearm.seek 1 + 800 counts Elbow at 1, Forearm at garage + 628 counts, Hand is closed @18:50:21.42 -> Thread.resume @18:50:21.43 Resuming after Elbow trajectoryErr at 4 at 18:47:08 @18:50:21.46 Elbow.seek :clear @18:50:22.04 Slide::Error in dave -- Elbow speedErr at 5 - 2338 counts @18:51:07.96 -> Thread.recover @18:51:07.97 Resuming after Elbow speedErr at 5 - 2338 counts at 18:50:21 @18:51:08.00 Elbow.seek :clear @18:51:08.40 Slide::Error in dave -- Elbow speedErr at 5 - 2324 counts @18:51:25.15 -> Arm.ready! @18:51:25.17 Forearm.reconfigure :loose @18:51:25.25 Forearm.home.seek :retract @18:51:26.65 Elbow.jog 1500 @18:51:27.16 Elbow.reconfigure :tight @18:51:27.23 Elbow.home.seek :clear Elbow at clear, Forearm retracted, Hand is closed @18:53:14.78 -> Arm.place @18:53:14.98 Elbow.seek 1 @18:53:16.15 Elbow.reconfigure :limp @18:53:16.22 Forearm.seek 1 @18:53:17.25 Elbow.hold @18:53:17.34 Elbow.reconfigure :tight Arm at elevator, Hand is closed @18:53:45.93 -> Arm.ready! @18:53:45.96 Forearm.reconfigure :loose @18:53:46.03 Forearm.home.seek :retract @18:53:47.63 Elbow.jog 1500 @18:53:48.05 Elbow.reconfigure :tight @18:53:48.12 Elbow.home.seek :clear Elbow at clear, Forearm retracted, Hand is closed @18:53:55.62 -> CC Collection Clamp contains sh1 puck from tube 2 @18:54:17.70 -> Arm.CC @18:54:17.85 Elbow.seek :CC @18:54:19.92 Elbow.reconfigure :loose @18:54:20.04 Forearm.reconfigure :tight @18:54:20.10 Forearm.seek CC @18:54:20.90 Slide::Error in dave -- Forearm speedErr at garage + 4509 counts @18:56:40.12 -> Arm.reset 0 @18:56:54.86 -> Thread.recover @18:56:54.87 Resuming after Forearm speedErr at garage + 4509 counts at 18:54:20 @18:56:54.89 Forearm.seek CC @18:56:55.24 Slide::Error in dave -- Forearm speedErr at garage + 4469 counts @18:57:09.03 -> Arm Elbow at CC, Forearm at garage + 4668 counts, Hand is closed @18:57:34.10 -> Arm.garage @18:57:34.24 Forearm.reconfigure :loose @18:57:34.31 Forearm.seek :retract @18:57:35.05 Elbow.reconfigure :tight @18:57:35.12 Elbow.seek :garage @18:57:37.96 Elbow.reconfigure :loose @18:57:38.08 Forearm.reconfigure :tight @18:57:38.15 Forearm.seek :garage @18:57:38.95 Slide::Error in dave -- Forearm speedErr at garage + 4704 counts @18:58:04.65 -> Arm.ready! @18:58:04.68 Forearm.reconfigure :loose @18:58:04.75 Forearm.home.seek :retract @18:58:06.07 Elbow.jog 1500 @18:58:06.51 Elbow.reconfigure :loose @18:58:06.58 Elbow.home.seek :clear Elbow at clear, Forearm retracted, Hand is closed @18:58:22.28 -> Hand.open @18:58:22.29 Hand.open @18:58:29.37 I2C::Request::Timeout in dave -- No Response to I2C::Servo::Release3Request[68:->26] @18:58:29.39 Retry #1 of 3 @18:58:29.47 I2C::UnexpectedReply in core -- I2C::Servo::Release3Reply[68:26->20] aborted @18:58:36.51 I2C::Request::Timeout in dave -- No Response to I2C::Servo::Release3Request[44:->26] @18:58:36.53 Retry #2 of 3 @18:58:36.60 I2C::UnexpectedReply in core -- I2C::Servo::Release3Reply[44:26->20] aborted @18:58:43.63 I2C::Request::Timeout in dave -- No Response to I2C::Servo::Release3Request[4E:->26] @18:58:43.65 Retry #3 of 3 @18:58:43.72 I2C::UnexpectedReply in core -- I2C::Servo::Release3Reply[4E:26->20] aborted @18:58:50.77 I2C::Request::Timeout in dave -- Hand could not finish opening because No Response to I2C::Servo::Release3Request[3D:->26] @19:00:07.97 -> Arm.garage @19:00:08.12 Elbow.reconfigure :tight @19:00:08.19 Elbow.seek :garage @19:00:09.54 Elbow.reconfigure :loose @19:00:09.66 Forearm.reconfigure :tight @19:00:09.72 Forearm.seek :garage @19:00:10.54 Slide::Error in dave -- Forearm speedErr at garage + 4714 counts @19:00:27.46 -> Hand.open @19:00:27.48 Hand.open @19:00:27.56 I2C::UnexpectedReply in core -- I2C::Servo::Release3Reply[3D:26->20] aborted @19:00:34.59 I2C::Request::Timeout in dave -- No Response to I2C::Servo::Release3Request[11:->26] @19:00:34.61 Retry #1 of 3 @19:00:34.68 I2C::UnexpectedReply in core -- I2C::Servo::Release3Reply[11:26->20] aborted @19:00:41.72 I2C::Request::Timeout in dave -- No Response to I2C::Servo::Release3Request[42:->26] @19:00:41.74 Retry #2 of 3 @19:00:41.81 I2C::UnexpectedReply in core -- I2C::Servo::Release3Reply[42:26->20] aborted @19:00:48.85 I2C::Request::Timeout in dave -- No Response to I2C::Servo::Release3Request[06:->26] @19:00:48.87 Retry #3 of 3 @19:00:48.94 I2C::UnexpectedReply in core -- I2C::Servo::Release3Reply[06:26->20] aborted @19:00:56.01 I2C::Request::Timeout in dave -- Hand could not finish opening because No Response to I2C::Servo::Release3Request[40:->26] @19:01:05.63 -> Flush FlushPuck is in FlushPuck::Garage @19:01:08.03 -> CC Collection Clamp contains sh1 puck from tube 2 @19:01:09.86 -> PC Processing Clamp open @19:01:44.58 -> MainThread.recover @19:01:44.60
Recovering Thread after undefined method `amount' for nil:NilClass at 18:23:32 # @19:01:44.86 Forearm.reconfigure :loose @19:01:44.93 Forearm.seek :retract @19:01:45.71 Elbow.reconfigure :tight @19:01:45.79 I2C::UnexpectedReply in core -- I2C::Servo::Release3Reply[40:26->20] @19:01:45.82 Elbow.seek 1 @19:01:47.79 Forearm.reconfigure :tight @19:01:47.87 Forearm.seek 1 + 800 counts @19:01:49.00 SE.reconfigure :pick @19:01:49.09 SE.seek :beamBreak @19:01:50.30 SE.configure :default @19:01:50.49 Elbow.reconfigure :limp @19:01:50.57 Forearm.reconfigure :loose @19:01:50.64 Forearm.seek 1 @19:01:52.97 Slide::Error in MAIN -- Forearm trajectoryErr at 1 @19:01:52.98 Retry #1 of 3 @19:01:53.10 Forearm.seek :retract @19:01:54.17 Elbow.reconfigure :loose @19:01:54.25 Elbow.seek 1 @19:01:54.46 Elbow.reconfigure :limp @19:01:54.54 Forearm.seek 1 @19:01:58.30 Slide::Error in MAIN -- Forearm trajectoryErr at 1 @19:01:58.31 Retry #2 of 3 @19:01:58.44 Forearm.seek :retract @19:01:59.49 Elbow.reconfigure :loose @19:01:59.56 Elbow.seek 1 @19:01:59.66 Elbow.reconfigure :limp @19:01:59.74 Forearm.seek 1 @19:02:02.84 Slide::Error in MAIN -- Forearm trajectoryErr at 1 @19:02:02.86 Retry #3 of 3 @19:02:02.98 Forearm.seek :retract @19:02:04.07 Elbow.reconfigure :loose @19:02:04.14 Elbow.seek 1 @19:02:04.23 Elbow.reconfigure :limp @19:02:04.32 Forearm.seek 1 @19:02:07.38 Slide::Error in MAIN -- Forearm trajectoryErr at 1 @19:02:07.39 Waiting to Resume from Checkpoint "Forearm trajectoryErr at 1" @19:02:07.43 BadNews.email "Forearm trajectoryErr at 1",Subject: "Slide::Error in MAIN" @19:02:11.42 upsync @19:02:16.36 -> Hand Hand is opening @19:02:19.98 -> Arm Arm at elevator, Hand is opening @19:02:27.53 -> Hand.open @19:02:27.54 Hand.open Hand is opened @19:02:32.27 -> Arm Arm at elevator, Hand is opened @19:02:42.86 -> MainThread.recover @19:02:42.89
Recovering Thread after Forearm trajectoryErr at 1 at 19:02:07 # @19:02:43.10 Elbow.reconfigure :tight @19:02:43.18 Elbow.seek 1 @19:02:43.40 Forearm.reconfigure :tight @19:02:43.48 Forearm.seek 1 + 800 counts @19:02:43.94 SE.reconfigure :pick @19:02:44.03 SE.seek :beamBreak @19:02:44.43 Slide::Error in MAIN -- Storage Elevator overCurrent at 17.95 Pucks below Top Plate @19:02:44.45 Waiting to Resume from Checkpoint "Storage Elevator overCurrent at 17.95 Pucks below Top Plate" @19:02:44.48 BadNews.email "Storage Elevator overCurrent at 17.95 Pucks below Top Plate",Subject: "Slide::Error in MAIN" @19:02:48.86 upsync @19:03:15.06 -> SE Storage Elevator at 20.76 Pucks below Top Plate @19:03:18.38 -> SC Storage Carousel at 1 @19:03:28.27 -> Puck.count @19:03:28.37 SE.reconfigure :default @19:03:28.46 SE.seek :down @19:03:29.11 SE.stop @19:03:29.52 Forearm.reconfigure :loose @19:03:29.59 Forearm.seek :retract @19:03:30.51 SC.reconfigure :tight @19:03:30.59 SC.seek 1 @19:03:30.67 SC.configure :limp @19:03:30.86 Elbow.seek 1 @19:03:31.08 Forearm.reconfigure :tight @19:03:31.16 Forearm.seek 1 + 800 counts @19:03:32.21 SE.reconfigure :place @19:03:32.29 SE.seek :beamBreak @19:03:33.81 SE.configure :default @19:03:33.95 SE.seek :down @19:03:34.74 SE.stop @19:03:35.08 Forearm.reconfigure :loose @19:03:35.16 Forearm.seek :retract @19:03:36.11 SC.reconfigure :tight @19:03:36.18 SC.seek 2 @19:03:39.80 SC.configure :limp @19:03:39.99 Elbow.seek 2 @19:03:40.29 Forearm.reconfigure :tight @19:03:40.37 Forearm.seek 2 + 800 counts @19:03:41.40 SE.reconfigure :place @19:03:41.48 SE.seek :beamBreak @19:03:44.62 SE.configure :default @19:03:44.75 SE.seek :down @19:03:45.89 Forearm.reconfigure :loose @19:03:45.96 Forearm.seek :retract @19:03:47.38 SE.stop @19:03:47.43 SC.reconfigure :tight @19:03:48.08 SC.seek 3 @19:03:51.72 SC.configure :limp @19:03:51.91 Elbow.seek 3 @19:03:52.30 Forearm.reconfigure :tight @19:03:52.43 Forearm.seek 3 + 800 counts @19:03:53.48 SE.reconfigure :place @19:03:53.56 SE.seek :beamBreak @19:03:54.79 SE.configure :default @19:03:54.92 SE.seek :down @19:03:55.52 SE.stop @19:03:56.06 Forearm.reconfigure :loose @19:03:56.13 Forearm.seek :retract @19:03:57.09 SC.reconfigure :tight @19:03:57.16 SC.seek 4 @19:04:00.82 SC.configure :limp @19:04:01.00 Elbow.seek 4 @19:04:01.21 Forearm.reconfigure :tight @19:04:01.29 Forearm.seek 4 + 800 counts @19:04:02.33 SE.reconfigure :place @19:04:02.41 SE.seek :beamBreak @19:04:03.68 SE.configure :default @19:04:03.81 SE.seek :down @19:04:04.41 SE.stop @19:04:04.95 Forearm.reconfigure :loose @19:04:05.02 Forearm.seek :retract @19:04:06.00 SC.reconfigure :tight @19:04:06.07 SC.seek 5 @19:04:09.66 SC.configure :limp @19:04:09.84 Elbow.seek 5 @19:04:10.21 Forearm.reconfigure :tight @19:04:10.28 Forearm.seek 5 + 800 counts @19:04:11.33 SE.reconfigure :place @19:04:11.42 SE.seek :beamBreak @19:04:12.76 SE.configure :default @19:04:12.89 SE.seek :down @19:04:13.56 SE.stop @19:04:14.37 Forearm.reconfigure :loose @19:04:14.44 Forearm.seek :retract @19:04:15.47 SC.reconfigure :tight @19:04:15.54 SC.seek 6 @19:04:19.47 SC.configure :limp @19:04:19.65 Elbow.seek 6 @19:04:20.13 Forearm.reconfigure :tight @19:04:20.20 Forearm.seek 6 + 800 counts @19:04:21.21 SE.reconfigure :place @19:04:21.29 SE.seek :beamBreak @19:04:22.59 SE.configure :default @19:04:22.71 SE.seek :down @19:04:23.28 SE.stop @19:04:23.86 Forearm.reconfigure :loose @19:04:23.93 Forearm.seek :retract @19:04:24.90 SC.reconfigure :tight @19:04:24.97 SC.seek 7 @19:04:28.66 SC.configure :limp @19:04:28.84 Elbow.seek 7 @19:04:29.06 Forearm.reconfigure :tight @19:04:29.14 Forearm.seek 7 + 800 counts @19:04:30.10 SE.reconfigure :place @19:04:30.18 SE.seek :beamBreak @19:04:31.49 SE.configure :default @19:04:31.62 SE.seek :down @19:04:32.20 SE.stop @19:04:32.76 Forearm.reconfigure :loose @19:04:32.83 Forearm.seek :retract {1 => 20, 2 => 1, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22} @19:04:45.64 -> Arm Elbow at 7, Forearm retracted, Hand is opened @19:04:53.92 -> Thread.resume @19:04:53.94 Resuming after Hand could not finish opening because No Response to I2C::Servo::Release3Request[40:->26] at 19:00:55 @19:04:53.96 Hand.open Hand is opened @19:05:01.26 -> Hand.open Hand is opened @19:05:12.33 -> Thread.resume @19:05:12.34 Resuming after Hand could not finish opening because No Response to I2C::Servo::Release3Request[40:->26] at 19:00:55 @19:05:12.36 Hand.open Hand is opened @19:05:18.55 -> MainThread.resume @19:05:18.57
Resuming Thread after Storage Elevator overCurrent at 17.95 Pucks below Top Plate at 19:02:44 # @19:05:18.61 SE.reconfigure :pick @19:05:18.76 SE.seek :beamBreak @19:05:19.70 SE.configure :default @19:05:19.78 Puck::Error in MAIN -- 22 pucks loaded in tube 1 when expecting 20 @19:05:19.80 Waiting to Resume from Checkpoint "Storage Elevator overCurrent at 17.95 Pucks below Top Plate" @19:05:19.82 BadNews.email "22 pucks loaded in tube 1 when expecting 20",Subject: "Puck::Error in MAIN" @19:05:24.16 upsync @19:06:13.15 -> CC.unloadPuck @19:06:13.25 Storing sh1 puck in Collection Clamp to tube 1 @19:06:13.42 SE.seek :down @19:06:14.20 SC.reconfigure :tight @19:06:14.27 SC.seek 1 @19:06:33.88 SC.configure :limp @19:06:34.06 Elbow.seek 1 @19:06:34.49 Forearm.reconfigure :tight @19:06:34.56 Forearm.seek 1 + 800 counts @19:06:35.65 SE.reconfigure :pick @19:06:35.73 SE.seek :beamBreak @19:06:36.90 SE.configure :default @19:06:37.08 Elbow.reconfigure :limp @19:06:37.15 Forearm.reconfigure :loose @19:06:37.22 Forearm.seek 1 @19:06:39.58 Slide::Error in dave -- Forearm trajectoryErr at 1 @19:06:39.60 Retry #1 of 3 @19:06:39.71 Forearm.seek :retract @19:06:40.76 Elbow.reconfigure :loose @19:06:40.84 Elbow.seek 1 @19:06:41.07 Elbow.reconfigure :limp @19:06:41.15 Forearm.seek 1 @19:06:44.21 Slide::Error in dave -- Forearm trajectoryErr at 1 @19:06:44.23 Retry #2 of 3 @19:06:44.35 Forearm.seek :retract @19:06:45.40 Elbow.reconfigure :loose @19:06:45.48 Elbow.seek 1 @19:06:45.57 Elbow.reconfigure :limp @19:06:45.65 Forearm.seek 1 @19:06:48.70 Slide::Error in dave -- Forearm trajectoryErr at 1 @19:06:48.72 Retry #3 of 3 @19:06:48.83 Forearm.seek :retract @19:06:49.87 Elbow.reconfigure :loose @19:06:49.94 Elbow.seek 1 @19:06:50.04 Elbow.reconfigure :limp @19:06:50.12 Forearm.seek 1 @19:06:53.19 Slide::Error in dave -- Forearm trajectoryErr at 1 @19:06:53.85 Retry #1 of 4 @19:06:53.89 SE.seek :down @19:06:54.96 Elbow.reconfigure :tight @19:06:55.04 Elbow.seek 1 @19:06:55.32 Forearm.reconfigure :tight @19:06:55.40 Forearm.seek 1 + 800 counts @19:06:55.76 SE.reconfigure :pick @19:06:55.85 SE.seek :beamBreak @19:06:57.06 SE.configure :default @19:06:57.24 Elbow.reconfigure :limp @19:06:57.32 Forearm.reconfigure :loose @19:06:57.40 Forearm.seek 1 @19:06:59.78 Slide::Error in dave -- Forearm trajectoryErr at 1 @19:06:59.79 Retry #1 of 3 @19:06:59.91 Forearm.seek :retract @19:07:00.93 Elbow.reconfigure :loose @19:07:01.01 Elbow.seek 1 @19:07:01.10 Elbow.reconfigure :limp @19:07:01.18 Forearm.seek 1 @19:07:02.30 Elbow.hold @19:07:02.39 Elbow.reconfigure :tight @19:07:02.52 SE.seek 19.09 Pucks below Top Plate @19:07:03.12 Forearm.force -20 @19:07:03.19 Forearm.seek 1 + 317 counts @19:07:03.50 Forearm.reconfigure :tight @19:07:03.57 Forearm.seek 1 + 800 counts @19:07:03.91 SE.seek 20.09 Pucks below Top Plate @19:07:04.63 Forearm.reconfigure :loose @19:07:04.70 Forearm.seek :retract @19:07:05.71 Elbow.seek CC @19:07:07.34 Elbow.reconfigure :loose @19:07:07.46 Forearm.seek CC @19:07:08.52 Hand.close @19:07:09.23 CC.seek :open @19:07:13.44 Forearm.seek :retract @19:07:14.62 Elbow.reconfigure :tight @19:07:14.69 Elbow.seek 1 @19:07:16.41 Elbow.reconfigure :limp @19:07:17.06 Forearm.seek 1 @19:07:18.25 Elbow.hold @19:07:18.34 Elbow.reconfigure :tight @19:07:18.41 Hand.open @19:07:19.34 Forearm.force -20 @19:07:19.40 Forearm.seek 1 + 314 counts @19:07:19.80 Forearm.reconfigure :tight @19:07:19.88 Forearm.seek 1 + 800 counts @19:07:20.18 SE.seek :down @19:07:20.98 SE.stop @19:07:21.32 Forearm.reconfigure :loose @19:07:21.39 Forearm.seek :retract @19:08:20.10 -> CC Collection Clamp open @19:08:22.53 -> PC Processing Clamp open @19:08:31.37 -> puckCount 1 @19:08:31.39 NoMethodError in dave -- undefined method `puckCount' for CmdServer::Client:Class @19:09:43.17 -> Puck.Count 1 @19:09:43.19 NoMethodError in dave -- undefined method `Count' for Puck:Module @19:10:29.65 -> Puck.count 1 @19:10:29.73 SE.seek :down @19:10:29.89 SE.stop @19:10:30.01 SC.reconfigure :tight @19:10:30.07 SC.seek 1 @19:10:30.16 SC.configure :limp @19:10:30.34 Elbow.seek 1 @19:10:30.62 Forearm.reconfigure :tight @19:10:30.70 Forearm.seek 1 + 800 counts @19:10:31.81 SE.reconfigure :place @19:10:31.89 SE.seek :beamBreak @19:10:33.24 SE.configure :default @19:10:33.36 SE.seek :down @19:10:34.04 SE.stop @19:10:34.50 Forearm.reconfigure :loose @19:10:34.57 Forearm.seek :retract {1 => 21, 2 => 1, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22} @19:10:44.79 -> Thread.resume @19:10:44.80 Resuming after Forearm trajectoryErr at 1 at 19:06:58 @19:10:44.83 Forearm.seek 1 @19:10:45.83 Elbow.hold @19:10:45.98 NoMethodError in dave -- undefined method `amount' for nil:NilClass @19:10:53.30 -> MainTthread.resume @19:10:53.32 NameError in dave -- uninitialized constant MainTthread @19:11:02.98 -> MainThread.resume @19:11:03.01
Resuming Thread after Storage Elevator overCurrent at 17.95 Pucks below Top Plate at 19:02:44 # @19:11:03.05 SE.reconfigure :pick @19:11:03.19 SE.seek :beamBreak @19:11:03.84 Slide::Error in MAIN -- Storage Elevator speedErr at 20.63 Pucks below Top Plate @19:11:03.85 Waiting to Resume from Checkpoint "Storage Elevator speedErr at 20.63 Pucks below Top Plate" @19:11:03.89 BadNews.email "Storage Elevator speedErr at 20.63 Pucks below Top Plate",Subject: "Slide::Error in MAIN" @19:11:08.23 upsync @19:11:20.15 -> CC Collection Clamp open @19:12:11.26 -> Thread.recover @19:12:11.29 Resuming after undefined method `amount' for nil:NilClass at 19:10:45 @19:12:11.48 Elbow.seek 1 @19:12:11.72 Forearm.reconfigure :tight @19:12:11.79 Forearm.seek 1 + 800 counts @19:12:12.21 SE.seek :beamBreak @19:12:13.16 SE.configure :default @19:12:13.35 Elbow.reconfigure :limp @19:12:13.44 Forearm.reconfigure :loose @19:12:13.51 Forearm.seek 1 @19:12:13.95 Elbow.hold @19:12:14.05 Elbow.reconfigure :tight @19:12:14.18 SE.seek 20.09 Pucks below Top Plate @19:12:14.75 Forearm.force -20 @19:12:14.83 Forearm.seek 1 + 298 counts @19:12:15.17 Forearm.reconfigure :tight @19:12:15.24 Forearm.seek 1 + 800 counts @19:12:15.67 SE.seek 21.09 Pucks below Top Plate @19:12:16.39 Forearm.reconfigure :loose @19:12:16.46 Forearm.seek :retract @19:12:17.45 Elbow.seek CC @19:12:19.07 Elbow.reconfigure :loose @19:12:19.20 Forearm.seek CC @19:12:20.20 Hand.close @19:12:20.97 CC.seek :open @19:12:21.32 Forearm.seek :retract @19:12:22.34 Elbow.reconfigure :tight @19:12:22.41 Elbow.seek 1 @19:12:24.19 Elbow.reconfigure :limp @19:12:24.26 Forearm.seek 1 @19:12:25.92 Elbow.hold @19:12:26.01 Elbow.reconfigure :tight @19:12:26.08 Hand.open @19:12:26.97 Forearm.force -20 @19:12:27.03 Forearm.seek 1 + 310 counts @19:12:27.50 Forearm.reconfigure :tight @19:12:27.57 Forearm.seek 1 + 800 counts @19:12:27.98 SE.seek :down @19:12:28.68 SE.stop @19:12:29.12 Forearm.reconfigure :loose @19:12:29.20 Forearm.seek :retract @19:12:56.91 -> MainThread.recover @19:12:56.93
Recovering Thread after Storage Elevator speedErr at 20.63 Pucks below Top Plate at 19:11:03 # @19:12:57.13 Elbow.seek 1 @19:12:57.36 Forearm.reconfigure :tight @19:12:57.43 Forearm.seek 1 + 800 counts @19:12:58.62 SE.reconfigure :pick @19:12:58.70 SE.seek :beamBreak @19:12:59.78 SE.configure :default @19:12:59.86 Puck::Error in MAIN -- 21 pucks loaded in tube 1 when expecting 22 @19:12:59.88 Waiting to Resume from Checkpoint "Storage Elevator speedErr at 20.63 Pucks below Top Plate" @19:12:59.90 BadNews.email "21 pucks loaded in tube 1 when expecting 22",Subject: "Puck::Error in MAIN" @19:13:03.67 upsync @19:13:33.92 -> MainThread.resume @19:13:33.94
Resuming Thread after Storage Elevator speedErr at 20.63 Pucks below Top Plate at 19:11:03 # @19:13:33.98 SE.reconfigure :pick @19:13:34.13 SE.seek :beamBreak @19:13:34.52 Slide::Error in MAIN -- Storage Elevator overCurrent at 19 Pucks below Top Plate @19:13:34.53 Waiting to Resume from Checkpoint "Storage Elevator overCurrent at 19 Pucks below Top Plate" @19:13:34.57 BadNews.email "Storage Elevator overCurrent at 19 Pucks below Top Plate",Subject: "Slide::Error in MAIN" @19:13:38.99 upsync @19:14:02.42 -> SE Storage Elevator at 20.76 Pucks below Top Plate @19:14:04.57 -> SC Storage Carousel at 1 @19:14:36.14 -> Puck.count 1 @19:14:36.24 SE.reconfigure :default @19:14:36.34 SE.seek :down @19:14:36.98 SE.stop @19:14:37.38 Forearm.reconfigure :loose @19:14:37.45 Forearm.seek :retract @19:14:38.44 SC.reconfigure :tight @19:14:38.51 SC.seek 1 @19:14:38.59 SC.configure :limp @19:14:38.78 Elbow.seek 1 @19:14:38.99 Forearm.reconfigure :tight @19:14:39.07 Forearm.seek 1 + 800 counts @19:14:40.25 SE.reconfigure :place @19:14:40.33 SE.seek :beamBreak @19:14:41.70 SE.configure :default @19:14:41.77 Puck::Error in dave -- 21 pucks loaded in tube 1 when expecting 22 @19:14:41.87 SE.seek :down @19:14:42.55 SE.stop @19:14:43.00 Forearm.reconfigure :loose @19:14:43.07 Forearm.seek :retract {1 => 21, 2 => 1, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22} @19:16:45.94 -> CC.loadPuck 2,2 @19:16:45.97 Loading 2 puck from tube 2 into Collection Clamp @19:16:46.24 SC.reconfigure :tight @19:16:46.31 SC.seek 2 @19:16:49.99 SC.configure :limp @19:16:50.17 Elbow.seek 2 @19:16:50.47 Forearm.reconfigure :tight @19:16:50.54 Forearm.seek 2 + 800 counts @19:16:51.60 SE.reconfigure :pick @19:16:51.68 SE.seek :beamBreak @19:16:54.57 SE.configure :default @19:16:54.75 Elbow.seek 2 @19:16:54.96 Forearm.seek 2 + 300 counts @19:16:55.21 Forearm.reconfigure :loose @19:16:55.28 Hand.close @19:16:56.10 SE.seek :down @19:16:57.00 Forearm.seek :retract @19:16:58.01 Elbow.seek CC @19:16:59.60 SE.stop @19:16:59.73 Elbow.reconfigure :loose @19:16:59.86 Forearm.reconfigure :tight @19:16:59.93 Forearm.seek CC @19:17:01.18 CC.seek :closed @19:17:05.18 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.97 @19:17:05.20 Hand.open @19:17:06.02 Forearm.reconfigure :loose @19:17:06.09 Forearm.seek :retract @19:17:10.75 -> CC Collection Clamp contains 2 puck from tube 2 @19:17:19.92 -> MainThread.resume @19:17:19.94
Resuming Thread after Storage Elevator overCurrent at 19 Pucks below Top Plate at 19:13:34 # @19:17:19.99 SE.reconfigure :pick @19:17:20.13 SE.seek :beamBreak @19:17:22.97 SE.configure :default @19:17:23.06 SE.seek :place @19:17:23.43 Puck::Error in MAIN -- 0 pucks loaded in tube 1 when expecting 21 @19:17:23.45 Waiting to Resume from Checkpoint "Storage Elevator overCurrent at 19 Pucks below Top Plate" @19:17:23.48 BadNews.email "0 pucks loaded in tube 1 when expecting 21",Subject: "Puck::Error in MAIN" @19:17:27.36 upsync @19:17:49.54 -> Puck.count @19:17:49.64 SE.seek :down @19:17:52.36 SE.stop @19:17:52.43 SC.reconfigure :tight @19:17:52.50 SC.seek 1 @19:17:56.11 SC.configure :limp @19:17:56.36 Elbow.reconfigure :tight @19:17:56.44 Elbow.seek 1 @19:17:58.84 Forearm.reconfigure :tight @19:17:58.92 Forearm.seek 1 + 800 counts @19:17:59.90 SE.reconfigure :place @19:17:59.98 SE.seek :beamBreak @19:18:01.39 SE.configure :default @19:18:01.52 SE.seek :down @19:18:02.22 SE.stop @19:18:02.66 Forearm.reconfigure :loose @19:18:02.73 Forearm.seek :retract @19:18:03.75 SC.reconfigure :tight @19:18:03.83 SC.seek 2 @19:18:07.66 SC.configure :limp @19:18:07.85 Elbow.seek 2 @19:18:08.14 Forearm.reconfigure :tight @19:18:08.22 Forearm.seek 2 + 800 counts @19:18:09.26 SE.reconfigure :place @19:18:09.34 SE.seek :beamBreak @19:18:12.17 SE.configure :default @19:18:12.29 SE.seek :down @19:18:13.43 Forearm.reconfigure :loose @19:18:13.50 Forearm.seek :retract @19:18:15.06 SE.stop @19:18:15.13 SC.reconfigure :tight @19:18:15.20 SC.seek 3 @19:18:18.79 SC.configure :limp @19:18:18.97 Elbow.seek 3 @19:18:19.35 Forearm.reconfigure :tight @19:18:19.42 Forearm.seek 3 + 800 counts @19:18:20.47 SE.reconfigure :place @19:18:20.55 SE.seek :beamBreak @19:18:21.81 SE.configure :default @19:18:21.93 SE.seek :down @19:18:22.52 SE.stop @19:18:23.07 Forearm.reconfigure :loose @19:18:23.14 Forearm.seek :retract @19:18:24.12 SC.reconfigure :tight @19:18:24.84 SC.seek 4 @19:18:28.46 SC.configure :limp @19:18:28.64 Elbow.seek 4 @19:18:28.86 Forearm.reconfigure :tight @19:18:28.94 Forearm.seek 4 + 800 counts @19:18:29.98 SE.reconfigure :place @19:18:30.06 SE.seek :beamBreak @19:18:31.35 SE.configure :default @19:18:31.48 SE.seek :down @19:18:32.08 SE.stop @19:18:32.62 Forearm.reconfigure :loose @19:18:32.69 Forearm.seek :retract @19:18:33.79 SC.reconfigure :tight @19:18:33.86 SC.seek 5 @19:18:37.39 SC.configure :limp @19:18:37.57 Elbow.seek 5 @19:18:37.91 Forearm.reconfigure :tight @19:18:37.98 Forearm.seek 5 + 800 counts @19:18:38.99 SE.reconfigure :place @19:18:39.07 SE.seek :beamBreak @19:18:40.31 SE.configure :default @19:18:40.44 SE.seek :down @19:18:41.02 SE.stop @19:18:41.58 Forearm.reconfigure :loose @19:18:41.65 Forearm.seek :retract @19:18:42.77 SC.reconfigure :tight @19:18:42.85 SC.seek 6 @19:18:46.80 SC.configure :limp @19:18:46.98 Elbow.seek 6 @19:18:47.36 Forearm.reconfigure :tight @19:18:47.43 Forearm.seek 6 + 800 counts @19:18:48.49 SE.reconfigure :place @19:18:48.58 SE.seek :beamBreak @19:18:49.81 SE.configure :default @19:18:49.93 SE.seek :down @19:18:51.09 SE.stop @19:18:51.34 Forearm.reconfigure :loose @19:18:51.41 Forearm.seek :retract @19:18:52.38 SC.reconfigure :tight @19:18:52.45 SC.seek 7 @19:18:56.10 SC.configure :limp @19:18:56.28 Elbow.seek 7 @19:18:56.49 Forearm.reconfigure :tight @19:18:56.57 Forearm.seek 7 + 800 counts @19:18:57.71 SE.reconfigure :place @19:18:57.79 SE.seek :beamBreak @19:18:59.10 SE.configure :default @19:18:59.23 SE.seek :down @19:18:59.83 SE.stop @19:19:00.36 Forearm.reconfigure :loose @19:19:00.44 Forearm.seek :retract {1 => 21, 2 => 0, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22} @19:19:10.31 -> Thread.recover @19:19:10.33 Resuming after undefined method `amount' for nil:NilClass at 19:10:45 @19:19:10.50 Elbow.seek 7 @19:19:10.73 Forearm.reconfigure :tight @19:19:10.80 Forearm.seek 7 + 800 counts @19:19:11.86 SE.reconfigure :pick @19:19:11.94 SE.seek :beamBreak @19:19:12.95 SE.configure :default @19:19:13.14 Elbow.reconfigure :limp @19:19:13.22 Forearm.reconfigure :loose @19:19:13.29 Forearm.seek 7 @19:19:13.68 Elbow.hold @19:19:13.77 Elbow.reconfigure :tight @19:19:13.90 SE.seek 21.14 Pucks below Top Plate @19:19:14.46 Forearm.force -20 @19:19:14.53 Forearm.seek 7 + 318 counts @19:19:14.93 Forearm.reconfigure :tight @19:19:15.01 Forearm.seek 7 + 800 counts @19:19:15.33 SE.seek 22.14 Pucks below Top Plate @19:19:16.06 Forearm.reconfigure :loose @19:19:16.13 Forearm.seek :retract @19:19:17.12 Elbow.seek CC @19:19:19.36 Elbow.reconfigure :loose @19:19:19.48 Forearm.seek CC @19:19:20.47 Hand.close @19:19:21.21 CC.seek :open @19:19:25.44 Forearm.seek :retract @19:19:26.46 Elbow.reconfigure :tight @19:19:26.54 Elbow.seek 7 @19:19:28.28 Elbow.reconfigure :limp @19:19:28.35 Forearm.seek 7 @19:19:29.52 Elbow.hold @19:19:29.60 Elbow.reconfigure :tight @19:19:29.67 Hand.open @19:19:30.59 Forearm.force -20 @19:19:30.65 Forearm.seek 7 + 318 counts @19:19:30.96 Forearm.reconfigure :tight @19:19:31.03 Forearm.seek 7 + 800 counts @19:19:31.39 SE.seek :down @19:19:32.00 SE.stop @19:19:32.53 Forearm.reconfigure :loose @19:19:32.61 Forearm.seek :retract @19:19:40.68 -> MainTthread.resume @19:19:40.71 NameError in dave -- uninitialized constant MainTthread @19:19:51.23 -> MainThread.resume @19:19:51.26
Resuming Thread after Storage Elevator overCurrent at 19 Pucks below Top Plate at 19:13:34 # @19:19:51.30 SE.reconfigure :pick @19:19:51.45 SE.seek :beamBreak @19:19:52.43 SE.configure :default @19:19:52.51 Puck::Error in MAIN -- 23 pucks loaded in tube 1 when expecting 22 @19:19:52.53 Waiting to Resume from Checkpoint "Storage Elevator overCurrent at 19 Pucks below Top Plate" @19:19:52.56 BadNews.email "23 pucks loaded in tube 1 when expecting 22",Subject: "Puck::Error in MAIN" @19:19:56.35 upsync @19:21:30.72 -> CC.unloadPuck 2,2 @19:21:30.81 Puck::Error in dave -- Collection Clamp is NOT CLOSED @19:21:35.46 -> CC Collection Clamp open @19:21:48.76 -> Thread.resume @19:21:48.78 Resuming (suspended since 19:16:45) @19:21:48.80 Loading 2 puck from tube 2 into Collection Clamp @19:21:49.02 SE.seek :down @19:21:50.38 SC.reconfigure :tight @19:21:50.45 SC.seek 2 @19:22:06.91 SC.configure :limp @19:22:07.09 Elbow.seek 2 @19:22:07.39 Forearm.reconfigure :tight @19:22:07.46 Forearm.seek 2 + 800 counts @19:22:08.52 SE.reconfigure :pick @19:22:08.60 SE.seek :beamBreak @19:22:11.46 SE.configure :default @19:22:11.53 SE.seek :pick @19:22:11.87 Puck::Error in dave -- Tube 2 is Empty @19:22:21.28 -> Arm.CC @19:22:21.46 Forearm.reconfigure :loose @19:22:21.55 Forearm.seek :retract @19:22:22.62 Elbow.seek :CC @19:22:24.29 Elbow.reconfigure :loose @19:22:24.43 Forearm.seek CC Arm at CC, Hand is opened @19:23:02.22 -> CC Collection Clamp open @19:23:05.57 -> CC.close @19:23:05.65 Arm::Error in dave -- Claim Arm, then retry closing Collection Clamp @19:23:21.13 -> Arm.ready! @19:23:21.18 Forearm.reconfigure :loose @19:23:21.26 Forearm.home.seek :retract @19:23:22.76 Hand.close @19:23:22.82 Elbow.jog 1500 @19:23:23.41 Elbow.reconfigure :loose @19:23:23.50 Elbow.home.seek :clear Elbow at clear, Forearm retracted, Hand is closed @19:23:34.96 -> CC.close @19:23:35.12 Arm::Error in dave -- Cannot hold puck in Collection Clamp because Hand is closed @19:23:43.93 -> Hand Hand is closed @19:24:20.39 -> Flush FlushPuck is in FlushPuck::Garage @19:24:28.74 -> Flush.CC /home/esp/esp2/lib/thread.rb:534: [BUG] rb_gc_mark(): unknown data type 0x19(0x165270) non object ruby 1.8.9 (2021-11-29 MBARI8esp8/0x8770 on patchlevel 352) [arm-linux-eabi] + ESPwhich DA_WCR_100825.rb + fn=DA_WCR_100825.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_100825.rb + [ -f /home/esp/esp2/mission/DA_WCR_100825.rb ] + realpath /home/esp/esp2/mission/DA_WCR_100825.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_100825.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_100825.rb @20:14:52.82UTC08-Oct-25
ESP2 v3.42 I2C Gateway v3.20 for core bus @20:14:55.56 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @20:14:59.37 Polling Can every 10:00 @20:14:59.42 Loading /home/esp/esp2local/mission/DA_WCR_100825.rb mission @20:14:59.48 Preparing to sleep until + 00:00:05 @20:14:59.50 GoodNews.email "Starting DA_WCR_100825 w/pucks in tube 3\n{}\nCTD data unavailable\nCan@20:14:59, 40% flow, 21.9C, 20% humidity, 14.7psia, 13.79V, 0.215A avg, 113.117Ah, 2.96W",Subject: "DA_WCR_100825 begun" @20:14:59.57
Asleep til + 00:00:05 @20:14:59.58 Continuing at 20:15 @20:14:59.84 Gate.power :core,:OFF @20:14:59.85 Gate.power :raw,:OFF @20:14:59.86 Shutdown @20:15:02.02 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_100825.rb:14\n{1 => 22,\n 2 => 2,\n 3 => 20,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@20:14:59, 40% flow, 21.9C, 20% humidity, 14.7psia, 13.79V, 0.208A avg, 113.117Ah, 2.87W",Subject: "Asleep until 20:15" @20:15:03.57 upsync @20:15:04.48
Powering core bus @20:15:06.03 upsync @20:15:09.93
Gate.power :raw,:ON @20:15:10.98 CS.configure :default @20:15:11.03 PS.configure :default @20:15:11.08 SS.configure :default @20:15:11.13 CC.configure :default @20:15:11.17 PC.configure :default @20:15:11.24 Forearm.configure :loose @20:15:11.29 Elbow.configure :loose @20:15:11.36 SC.configure :loose @20:15:11.41 SE.configure :default @20:15:11.45 CH.configure :default @20:15:11.49 PH.configure :default @20:15:11.53 All dwarf controllers running firmware version 3.85 @20:15:11.63 Valve.reset @20:15:11.86 Intake.select :close @20:15:11.91 Exhaust.select :close @20:15:11.94 CTV.dial :air @20:15:12.03 PSV.dial :air @20:15:12.07 CSV.dial :waste1 @20:15:12.21
Forearm.home.seek :retract @20:15:12.85 PS.home.seek 0ml @20:15:12.98 Hand.close @20:15:13.02
Elbow.reconfigure :loose @20:15:13.12 SSV.dial :spit,avoiding: :exhaust @20:15:13.14
Elbow.jog 1500 @20:15:13.37 CS.reconfigure :fast @20:15:13.41 SS.home.seek 0ml @20:15:13.48 CS.home.seek 0ml @20:15:13.65
Elbow.reconfigure :loose @20:15:13.70 Elbow.home.seek :clear @20:15:18.10 SE.home.seek :down @20:15:18.72 SC.reconfigure :tight @20:15:18.77 SC.home.seek :initial @20:15:22.03 PS.stop @20:15:47.46
SC.configure :limp @20:15:47.51 Forearm.seek :retract @20:15:47.57 Elbow.seek :hammer @20:15:48.63 Forearm.reconfigure :tight @20:15:48.69 Forearm.seek retracted @20:15:48.95 CC.home.seek :open @20:15:48.96 PC.home.seek :open @20:15:52.50
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @20:15:52.52 Notice.email "At 20:15:52 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_100825.rb:14\n{1 => 22,\n 2 => 2,\n 3 => 20,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@20:15:52, 39% flow, 21.8C, 19% humidity, 14.7psia, 13.71V, 0.284A avg, 113.124Ah, 3.89W",Subject: "Started DA_WCR_100825 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @20:15:52.54
Starting initialPurge @20:15:52.57 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:15:52.63 Hand.open @20:15:53.44 Elbow.reconfigure :tight @20:15:53.49 Elbow.seek FlushPuck::Garage @20:15:55.43 Elbow.reconfigure :loose @20:15:55.55 Forearm.reconfigure :loose @20:15:55.60 Forearm.seek :garage @20:15:56.59 Hand.close @20:15:56.61 upsync @20:15:57.42
Forearm.seek :retract @20:15:58.43 Elbow.reconfigure :tight @20:15:58.48 Elbow.seek CC @20:16:01.28 Elbow.reconfigure :loose @20:16:01.39 Forearm.reconfigure :tight @20:16:01.44 Forearm.seek CC @20:16:02.75 CC.seek :closed @20:16:06.77 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.08 @20:16:06.84 Hand.open @20:16:07.61 Forearm.reconfigure :loose @20:16:07.66 Forearm.seek :retract @20:16:08.69 Flushing the Collection Valve::Manifold @20:16:08.81 CSV.dial CSR @20:16:10.10 Pulling 4.999ml of CSR.flush @20:16:10.12 CSR.select :flush @20:16:10.30 CS.reconfigure :default @20:16:10.39 CS.seek 5ml @20:16:24.49 delay 5 seconds @20:16:29.50 CSR.select :air @20:16:29.67 CS.reconfigure :fast @20:16:29.72 CS.seek 7.5ml @20:16:36.45 CSV.dial :waste @20:16:37.72 CS.reconfigure :default @20:16:37.77 CS.seek 0ml @20:16:57.89 Purging external Intake @20:16:58.01 CSV.dial :flush @20:16:58.46 CS.reconfigure :fast @20:16:58.51 CS.seek 10ml @20:17:22.30 delay 5 seconds @20:17:27.32 CSV.dial CBV @20:17:27.35
CBV.dial CSV @20:17:28.15 CTV.dial :intake @20:17:28.91 Intake.select :open @20:17:29.02 CS.seek 0ml @20:17:52.70 Intake.select :close @20:17:52.81 CTV.dial :air @20:17:53.57 Flushing upper manifold @20:17:53.68 CSV.dial :flush @20:17:54.40 CS.seek 5ml @20:18:06.70 delay 5 seconds @20:18:11.72 CSV.dial :air @20:18:12.22 CS.seek 7ml @20:18:17.70 CSV.dial CBV @20:18:17.73
CBV.dial CSV @20:18:18.75 CTV.dial :air @20:18:18.83 CS.seek 0ml @20:18:35.55 delay 7 seconds @20:18:42.57 CS.seek 3ml @20:18:50.36 CSV.dial :waste @20:18:50.82 CS.seek 0ml @20:18:58.62 CSV.dial :flush @20:18:59.07 CS.seek 3ml @20:19:06.80 delay 5 seconds @20:19:11.82 CSV.dial :air @20:19:12.33 CS.seek 4.999ml @20:19:17.80 CSV.dial :air @20:19:17.97 CS.seek 0ml @20:19:30.20 delay 7 seconds @20:19:37.23 CS.seek 3ml @20:19:44.95 CSV.dial :waste @20:19:45.72 CS.seek 0ml @20:19:53.42 Garaging FlushPuck in Collection Clamp @20:19:53.49 Elbow.reconfigure :tight @20:19:53.54 Elbow.seek CC @20:19:53.66 Elbow.reconfigure :loose @20:19:53.76 Forearm.seek CC @20:19:54.75 Hand.close @20:19:55.55 CC.seek :open @20:20:00.04 Forearm.seek :retract @20:20:01.10 Elbow.reconfigure :tight @20:20:01.14 Elbow.seek :garage @20:20:04.02 Elbow.reconfigure :loose @20:20:04.12 Forearm.reconfigure :tight @20:20:04.17 Forearm.seek :garage @20:20:05.32 Hand.open @20:20:06.11 Forearm.reconfigure :loose @20:20:06.15 Forearm.seek :retract @20:20:07.21 da sampling at most 1000ml, wcr at most 1000ml @20:20:07.24 DAfans.email "Started da in tube 3 at 20:20:07UTC08-Oct-25\n{1 => 22,\n 2 => 2,\n 3 => 20,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@20:15:52, 39% flow, 21.8C, 19% humidity, 14.7psia, 13.71V, 0.284A avg, 113.124Ah, 3.89W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @20:20:07.27
Loading sh1 puck from tube 3 into Collection Clamp @20:20:07.53 SC.reconfigure :tight @20:20:07.58 SC.seek 3 @20:20:11.13 upsync @20:20:11.23
SC.configure :limp @20:20:11.46 Elbow.reconfigure :tight @20:20:11.51 Elbow.seek 3 @20:20:13.48 Forearm.reconfigure :tight @20:20:13.53 Forearm.seek 3 + 800 counts @20:20:14.50 SE.reconfigure :pick @20:20:14.56 SE.seek :beamBreak @20:20:15.76 SE.configure :default @20:20:15.92 Elbow.seek 3 @20:20:16.22 Forearm.seek 3 + 300 counts @20:20:16.48 Forearm.reconfigure :loose @20:20:16.53 Hand.close @20:20:17.31 SE.seek :down @20:20:17.95
Forearm.seek :retract @20:20:18.28 SE.stop @20:20:18.97
Elbow.seek CC @20:20:20.59 Elbow.reconfigure :loose @20:20:20.69 Forearm.reconfigure :tight @20:20:20.73 Forearm.seek CC @20:20:22.06 CC.seek :closed @20:20:26.08 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 2.00 @20:20:26.09 Hand.open @20:20:26.86 Forearm.reconfigure :loose @20:20:26.91 Forearm.seek :retract @20:20:27.95 collectSample 1000ml w/bubble point of 24psi @20:20:27.96 Priming 7ml @20:20:27.97 Intake.select :close @20:20:27.99 Exhaust.select :close @20:20:28.00 SS.hold @20:20:28.20 Calibrating pressure and temperature sensors @20:20:28.23 CTV.dial :air @20:20:28.31 SSV.dial :spit,avoiding: :exhaust @20:20:28.33 SS.reconfigure :fast @20:20:28.38 SS.seek 5ml @20:20:31.26 SSV.dial CBV @20:20:31.29
CBV.dial SSV @20:20:32.66 delay 3.8 seconds @20:20:36.59 Sensor limits: Intake max 104.9psia; Outlet max 112.0psia @20:20:36.60 SSV.dial :spit @20:20:37.93 SS.seek 0ml @20:20:40.93 SSV.dial CBV @20:20:42.27 CTV.dial :intake @20:20:43.04 SSV.dial CBV @20:20:43.06
CBV.dial SSV @20:20:43.29 Allowing 40s for 7.0ml of remaining 7.0ml @20:20:43.30 Intake.select :open @20:20:45.46 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:20:45.48 SS.reconfigure :inhaling 2.17ml/s @20:20:45.53 SS.seek 7ml @20:20:51.13 Stroke completed -- waiting for pressure to equalize ... @20:20:51.14 SS.reconfigure :exhale @20:20:51.18 SS.hold @20:20:52.85 Intake.select :close @20:20:53.00 SSV.dial :exhaust @20:20:53.77 Exhaust.select :open @20:20:53.89 SS.seek 0ml @20:20:57.88 Exhaust.select :close @20:20:57.99 Sampling 1000.0ml @20:20:58.00 Intake.select :close @20:20:58.01 Exhaust.select :close @20:20:58.03 SS.reconfigure :default @20:20:58.07 SS.hold @20:20:58.27 CTV.dial :intake @20:20:58.42 SSV.dial CBV @20:20:58.46
CBV.dial SSV @20:20:59.22 Allowing 130s for 25.0ml of remaining 1000.0ml @20:20:59.23 Intake.select :open @20:21:01.39 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:21:01.41 SS.reconfigure :inhaling 2.17ml/s @20:21:01.46 SS.seek 25ml @20:21:16.78 Stroke completed -- waiting for pressure to equalize ... @20:21:16.79 SS.reconfigure :exhale @20:21:16.84 SS.hold @20:21:23.35 Intake.select :close @20:21:23.50 SSV.dial :exhaust @20:21:24.27 Exhaust.select :open @20:21:24.38 SS.seek 0ml @20:21:33.28 Exhaust.select :close @20:21:33.39 SSV.dial CBV @20:21:33.41
CBV.dial SSV @20:21:34.19 Allowing 130s for 25.0ml of remaining 975.0ml @20:21:34.20 Intake.select :open @20:21:36.37 Intake @14.0psia, vacuum from 8.5 to 11.5psi @20:21:36.38 SS.reconfigure :inhaling 2.17ml/s @20:21:36.43 SS.seek 25ml @20:21:51.67 Stroke completed -- waiting for pressure to equalize ... @20:21:51.68 SS.reconfigure :exhale @20:21:51.73 SS.hold @20:21:58.11 Intake.select :close @20:21:58.26 SSV.dial :exhaust @20:21:59.02 Exhaust.select :open @20:21:59.13 SS.seek 0ml @20:22:07.99 Exhaust.select :close @20:22:08.10 SSV.dial CBV @20:22:08.12
CBV.dial SSV @20:22:08.91 Allowing 130s for 25.0ml of remaining 950.0ml @20:22:08.92 Intake.select :open @20:22:11.08 Intake @14.3psia, vacuum from 8.8 to 11.8psi @20:22:11.10 SS.reconfigure :inhaling 2.17ml/s @20:22:11.15 SS.seek 25ml @20:22:26.20 Stroke completed -- waiting for pressure to equalize ... @20:22:26.21 SS.reconfigure :exhale @20:22:26.26 SS.hold @20:22:33.71 Intake.select :close @20:22:33.86 SSV.dial :exhaust @20:22:35.07 Exhaust.select :open @20:22:35.18 SS.seek 0ml @20:22:44.02 Exhaust.select :close @20:22:44.13 SSV.dial CBV @20:22:44.15
CBV.dial SSV @20:22:44.96 Allowing 130s for 25.0ml of remaining 925.0ml @20:22:44.97 Intake.select :open @20:22:47.13 Intake @14.4psia, vacuum from 8.9 to 11.9psi @20:22:47.15 SS.reconfigure :inhaling 2.17ml/s @20:22:47.20 SS.seek 25ml @20:23:02.27 Stroke completed -- waiting for pressure to equalize ... @20:23:02.28 SS.reconfigure :exhale @20:23:02.32 SS.hold @20:23:09.69 Intake.select :close @20:23:09.84 SSV.dial :exhaust @20:23:10.63 Exhaust.select :open @20:23:10.74 SS.seek 0ml @20:23:19.89 Exhaust.select :close @20:23:20.00 SSV.dial CBV @20:23:20.02
CBV.dial SSV @20:23:20.82 Allowing 130s for 25.0ml of remaining 900.0ml @20:23:20.83 Intake.select :open @20:23:22.99 Intake @14.3psia, vacuum from 8.8 to 11.8psi @20:23:23.01 SS.reconfigure :inhaling 2.17ml/s @20:23:23.05 SS.seek 25ml @20:23:37.45 Stroke completed -- waiting for pressure to equalize ... @20:23:37.46 SS.reconfigure :exhale @20:23:37.51 SS.hold @20:23:45.10 Intake.select :close @20:23:45.25 SSV.dial :exhaust @20:23:46.03 Exhaust.select :open @20:23:46.14 SS.seek 0ml @20:23:54.94 Exhaust.select :close @20:23:55.05 SSV.dial CBV @20:23:55.07
CBV.dial SSV @20:23:55.88 Allowing 130s for 25.0ml of remaining 875.0ml @20:23:55.89 Intake.select :open @20:23:58.05 Intake @14.0psia, vacuum from 8.5 to 11.5psi @20:23:58.07 SS.reconfigure :inhaling 2.17ml/s @20:23:58.12 SS.seek 25ml @20:24:12.34 Stroke completed -- waiting for pressure to equalize ... @20:24:12.35 SS.reconfigure :exhale @20:24:12.40 SS.hold @20:24:22.14 Intake.select :close @20:24:22.72 SSV.dial :exhaust @20:24:23.51 Exhaust.select :open @20:24:23.62 SS.seek 0ml @20:24:32.49 Exhaust.select :close @20:24:32.60 SSV.dial CBV @20:24:32.62
CBV.dial SSV @20:24:33.41 Allowing 130s for 25.0ml of remaining 850.0ml @20:24:33.42 Intake.select :open @20:24:35.58 Intake @14.4psia, vacuum from 8.9 to 11.9psi @20:24:35.60 SS.reconfigure :inhaling 2.17ml/s @20:24:35.65 SS.seek 25ml @20:24:50.33 Stroke completed -- waiting for pressure to equalize ... @20:24:50.34 SS.reconfigure :exhale @20:24:50.38 SS.hold @20:25:00.06 Intake.select :close @20:25:00.20 SSV.dial :exhaust @20:25:00.97 Exhaust.select :open @20:25:01.08 SS.seek 0ml @20:25:09.88 Exhaust.select :close @20:25:09.99 SSV.dial CBV @20:25:10.01
CBV.dial SSV @20:25:10.78 Allowing 130s for 25.0ml of remaining 825.0ml @20:25:10.79 Intake.select :open @20:25:12.95 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:25:12.97 SS.reconfigure :inhaling 2.17ml/s @20:25:13.02 SS.seek 25ml @20:25:27.29 Stroke completed -- waiting for pressure to equalize ... @20:25:27.30 SS.reconfigure :exhale @20:25:27.35 SS.hold @20:25:37.54 Intake.select :close @20:25:37.69 SSV.dial :exhaust @20:25:38.45 Exhaust.select :open @20:25:38.56 SS.seek 0ml @20:25:47.42 Exhaust.select :close @20:25:47.53 SSV.dial CBV @20:25:47.55
CBV.dial SSV @20:25:48.33 Allowing 130s for 25.0ml of remaining 800.0ml @20:25:48.34 Intake.select :open @20:25:50.50 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:25:50.52 SS.reconfigure :inhaling 2.17ml/s @20:25:50.57 SS.seek 25ml @20:26:05.51 Stroke completed -- waiting for pressure to equalize ... @20:26:05.52 SS.reconfigure :exhale @20:26:05.57 SS.hold @20:26:16.70 Intake.select :close @20:26:16.85 SSV.dial :exhaust @20:26:17.61 Exhaust.select :open @20:26:17.72 SS.seek 0ml @20:26:26.58 Exhaust.select :close @20:26:26.69 SSV.dial CBV @20:26:26.71
CBV.dial SSV @20:26:27.48 Allowing 130s for 25.0ml of remaining 775.0ml @20:26:27.49 Intake.select :open @20:26:29.65 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:26:29.67 SS.reconfigure :inhaling 2.17ml/s @20:26:29.72 SS.seek 25ml @20:26:44.42 Stroke completed -- waiting for pressure to equalize ... @20:26:44.43 SS.reconfigure :exhale @20:26:44.47 SS.hold @20:26:57.04 Intake.select :close @20:26:57.19 SSV.dial :exhaust @20:26:57.95 Exhaust.select :open @20:26:58.06 SS.seek 0ml @20:27:07.68 Exhaust.select :close @20:27:07.79 SSV.dial CBV @20:27:07.81
CBV.dial SSV @20:27:08.59 Allowing 130s for 25.0ml of remaining 750.0ml @20:27:08.60 Intake.select :open @20:27:10.76 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:27:10.78 SS.reconfigure :inhaling 2.17ml/s @20:27:10.83 SS.seek 25ml @20:27:25.42 Stroke completed -- waiting for pressure to equalize ... @20:27:25.43 SS.reconfigure :exhale @20:27:25.47 SS.hold @20:27:39.77 Intake.select :close @20:27:39.92 SSV.dial :exhaust @20:27:40.68 Exhaust.select :open @20:27:40.79 SS.seek 0ml @20:27:49.72 Exhaust.select :close @20:27:49.83 SSV.dial CBV @20:27:49.85
CBV.dial SSV @20:27:50.62 Allowing 130s for 25.0ml of remaining 725.0ml @20:27:50.63 Intake.select :open @20:27:52.79 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:27:52.81 SS.reconfigure :inhaling 2.17ml/s @20:27:52.86 SS.seek 25ml @20:28:07.31 Stroke completed -- waiting for pressure to equalize ... @20:28:07.32 SS.reconfigure :exhale @20:28:07.36 SS.hold @20:28:23.98 Intake.select :close @20:28:24.14 SSV.dial :exhaust @20:28:24.90 Exhaust.select :open @20:28:25.02 SS.seek 0ml @20:28:33.87 Exhaust.select :close @20:28:33.98 SSV.dial CBV @20:28:34.01
CBV.dial SSV @20:28:34.78 Allowing 130s for 25.0ml of remaining 700.0ml @20:28:34.79 Intake.select :open @20:28:36.95 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:28:36.97 SS.reconfigure :inhaling 2.17ml/s @20:28:37.02 SS.seek 25ml @20:28:50.06 Vacuum too high -- waiting up to 40 seconds @20:28:50.08 SS.reconfigure :exhale @20:28:50.13 SS.hold @20:28:52.10 Intake @12.1psia, vacuum from 6.6 to 9.6psi @20:28:52.12 SS.reconfigure :inhaling 1.95ml/s @20:28:52.17 SS.seek 25ml @20:28:55.36 Stroke completed -- waiting for pressure to equalize ... @20:28:55.37 SS.reconfigure :exhale @20:28:55.85 SS.hold @20:29:10.23 Intake.select :close @20:29:10.38 SSV.dial :exhaust @20:29:11.15 Exhaust.select :open @20:29:11.27 SS.seek 0ml @20:29:20.53 Exhaust.select :close @20:29:20.63 SSV.dial CBV @20:29:20.66
CBV.dial SSV @20:29:21.43 Allowing 130s for 25.0ml of remaining 675.0ml @20:29:21.44 Intake.select :open @20:29:23.60 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:29:23.62 SS.reconfigure :inhaling 1.95ml/s @20:29:23.67 SS.seek 25ml @20:29:35.56 Vacuum too high -- waiting up to 40 seconds @20:29:35.58 SS.reconfigure :exhale @20:29:35.63 SS.hold @20:29:39.55 Intake @12.4psia, vacuum from 6.9 to 9.9psi @20:29:39.57 SS.reconfigure :inhaling 1.77ml/s @20:29:39.61 SS.seek 25ml @20:29:44.93 Stroke completed -- waiting for pressure to equalize ... @20:29:44.94 SS.reconfigure :exhale @20:29:44.99 SS.hold @20:30:01.28 Intake.select :close @20:30:01.43 SSV.dial :exhaust @20:30:02.20 Exhaust.select :open @20:30:02.31 SS.seek 0ml @20:30:11.46 Exhaust.select :close @20:30:11.57 SSV.dial CBV @20:30:11.60
CBV.dial SSV @20:30:12.38 Allowing 130s for 25.0ml of remaining 650.0ml @20:30:12.40 Intake.select :open @20:30:14.56 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:30:14.58 SS.reconfigure :inhaling 1.77ml/s @20:30:14.62 SS.seek 25ml @20:30:25.34 Vacuum too high -- waiting up to 40 seconds @20:30:25.35 SS.reconfigure :exhale @20:30:25.82 SS.hold @20:30:33.26 Intake @13.5psia, vacuum from 8.0 to 11.0psi @20:30:33.27 SS.reconfigure :inhaling 1.59ml/s @20:30:33.32 SS.seek 25ml @20:30:39.47 Vacuum too high -- waiting up to 40 seconds @20:30:39.48 SS.reconfigure :exhale @20:30:39.53 SS.hold @20:30:48.31 Intake @14.1psia, vacuum from 8.6 to 11.6psi @20:30:48.32 SS.reconfigure :inhaling 1.43ml/s @20:30:48.37 SS.seek 25ml @20:30:49.64 Stroke completed -- waiting for pressure to equalize ... @20:30:49.65 SS.reconfigure :exhale @20:30:49.70 SS.hold @20:30:53.22 Intake.select :close @20:30:53.38 SSV.dial :exhaust @20:30:54.13 Exhaust.select :open @20:30:54.24 SS.seek 0ml @20:31:03.02 Exhaust.select :close @20:31:03.13 SSV.dial CBV @20:31:03.15
CBV.dial SSV @20:31:03.93 Allowing 130s for 25.0ml of remaining 625.0ml @20:31:03.94 Intake.select :open @20:31:06.10 Intake @15.0psia, vacuum from 9.5 to 12.5psi @20:31:06.12 SS.reconfigure :inhaling 1.43ml/s @20:31:06.17 SS.seek 25ml @20:31:18.81 Vacuum too high -- waiting up to 40 seconds @20:31:18.82 SS.reconfigure :exhale @20:31:18.87 SS.hold @20:31:23.91 Intake @12.8psia, vacuum from 7.3 to 10.3psi @20:31:23.92 SS.reconfigure :inhaling 1.29ml/s @20:31:23.97 SS.seek 25ml @20:31:29.69 Vacuum too high -- waiting up to 40 seconds @20:31:29.70 SS.reconfigure :exhale @20:31:29.75 SS.hold @20:31:38.71 Intake @13.7psia, vacuum from 8.2 to 11.2psi @20:31:38.73 SS.reconfigure :inhaling 1.16ml/s @20:31:38.78 SS.seek 25ml @20:31:42.36 Stroke completed -- waiting for pressure to equalize ... @20:31:42.37 SS.reconfigure :exhale @20:31:42.42 SS.hold @20:31:49.88 Intake.select :close @20:31:50.03 SSV.dial :exhaust @20:31:50.79 Exhaust.select :open @20:31:50.90 SS.seek 0ml @20:31:59.83 Exhaust.select :close @20:31:59.94 SSV.dial CBV @20:31:59.96
CBV.dial SSV @20:32:00.74 Allowing 130s for 25.0ml of remaining 600.0ml @20:32:00.75 Intake.select :open @20:32:02.91 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:32:02.93 SS.reconfigure :inhaling 1.16ml/s @20:32:02.98 SS.seek 25ml @20:32:19.22 Vacuum too high -- waiting up to 40 seconds @20:32:19.23 SS.reconfigure :exhale @20:32:19.28 SS.hold @20:32:26.85 Intake @13.3psia, vacuum from 7.8 to 10.8psi @20:32:26.86 SS.reconfigure :inhaling 1.04ml/s @20:32:26.91 SS.seek 25ml @20:32:36.30 Stroke completed -- waiting for pressure to equalize ... @20:32:36.31 SS.reconfigure :exhale @20:32:36.36 SS.hold @20:32:50.74 Intake.select :close @20:32:50.89 SSV.dial :exhaust @20:32:51.68 Exhaust.select :open @20:32:51.79 SS.seek 0ml @20:33:01.01 Exhaust.select :close @20:33:01.11 SSV.dial CBV @20:33:01.14
CBV.dial SSV @20:33:01.93 Allowing 130s for 25.0ml of remaining 575.0ml @20:33:01.94 Intake.select :open @20:33:04.11 Intake @15.0psia, vacuum from 9.5 to 12.5psi @20:33:04.13 SS.reconfigure :inhaling 1.04ml/s @20:33:04.17 SS.seek 25ml @20:33:21.09 Vacuum too high -- waiting up to 40 seconds @20:33:21.10 SS.reconfigure :exhale @20:33:21.16 SS.hold @20:33:28.74 Intake @13.6psia, vacuum from 8.1 to 11.1psi @20:33:28.75 SS.reconfigure :inhaling 0.94ml/s @20:33:28.80 SS.seek 25ml @20:33:39.18 Vacuum too high -- waiting up to 40 seconds @20:33:39.19 SS.reconfigure :exhale @20:33:39.68 SS.hold @20:33:49.40 Intake @13.8psia, vacuum from 8.3 to 11.3psi @20:33:49.42 SS.reconfigure :inhaling 0.85ml/s @20:33:49.47 SS.seek 25ml @20:33:49.82 Stroke completed -- waiting for pressure to equalize ... @20:33:49.83 SS.reconfigure :exhale @20:33:49.87 SS.hold @20:33:56.27 Intake.select :close @20:33:56.85 SSV.dial :exhaust @20:33:57.63 Exhaust.select :open @20:33:57.75 SS.seek 0ml @20:34:07.08 Exhaust.select :close @20:34:07.19 SSV.dial CBV @20:34:07.22
CBV.dial SSV @20:34:08.01 Allowing 130s for 25.0ml of remaining 550.0ml @20:34:08.02 Intake.select :open @20:34:10.18 Intake @15.0psia, vacuum from 9.5 to 12.5psi @20:34:10.20 SS.reconfigure :inhaling 0.85ml/s @20:34:10.25 SS.seek 25ml @20:34:30.78 Vacuum too high -- waiting up to 40 seconds @20:34:30.79 SS.reconfigure :exhale @20:34:30.84 SS.hold @20:34:36.57 Intake @13.5psia, vacuum from 8.0 to 11.0psi @20:34:36.59 SS.reconfigure :inhaling 0.77ml/s @20:34:36.63 SS.seek 25ml @20:34:49.33 Stroke completed -- waiting for pressure to equalize ... @20:34:49.34 SS.reconfigure :exhale @20:34:49.38 SS.hold @20:35:05.69 Intake.select :close @20:35:05.84 SSV.dial :exhaust @20:35:06.63 Exhaust.select :open @20:35:06.74 SS.seek 0ml @20:35:16.05 Exhaust.select :close @20:35:16.16 SSV.dial CBV @20:35:16.18
CBV.dial SSV @20:35:16.96 Allowing 130s for 25.0ml of remaining 525.0ml @20:35:16.97 Intake.select :open @20:35:19.13 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:35:19.15 SS.reconfigure :inhaling 0.77ml/s @20:35:19.20 SS.seek 25ml @20:35:37.99 Vacuum too high -- waiting up to 40 seconds @20:35:38.00 SS.reconfigure :exhale @20:35:38.06 SS.hold @20:35:45.05 Intake @14.0psia, vacuum from 8.5 to 11.5psi @20:35:45.10 SS.reconfigure :inhaling 0.70ml/s @20:35:45.14 SS.seek 25ml @20:35:58.62 Vacuum too high -- waiting up to 40 seconds @20:35:58.63 SS.reconfigure :exhale @20:35:58.68 SS.hold @20:36:06.29 Intake @14.1psia, vacuum from 8.6 to 11.6psi @20:36:06.30 SS.reconfigure :inhaling 0.62ml/s @20:36:06.35 SS.seek 25ml @20:36:11.25 Stroke completed -- waiting for pressure to equalize ... @20:36:11.26 SS.reconfigure :exhale @20:36:11.31 SS.hold @20:36:20.28 Intake.select :close @20:36:20.44 SSV.dial :exhaust @20:36:21.21 Exhaust.select :open @20:36:21.32 SS.seek 0ml @20:36:30.27 Exhaust.select :close @20:36:30.38 SSV.dial CBV @20:36:30.40
CBV.dial SSV @20:36:31.20 Allowing 130s for 25.0ml of remaining 500.0ml @20:36:31.22 Intake.select :open @20:36:33.38 Intake @15.1psia, vacuum from 9.6 to 12.6psi @20:36:33.40 SS.reconfigure :inhaling 0.62ml/s @20:36:33.45 SS.seek 25ml @20:36:58.34 Vacuum too high -- waiting up to 40 seconds @20:36:58.35 SS.reconfigure :exhale @20:36:58.40 SS.hold @20:37:03.36 Intake @13.9psia, vacuum from 8.4 to 11.4psi @20:37:03.38 SS.reconfigure :inhaling 0.56ml/s @20:37:03.42 SS.seek 25ml @20:37:23.00 Stroke completed -- waiting for pressure to equalize ... @20:37:23.01 SS.reconfigure :exhale @20:37:23.05 SS.hold @20:37:37.84 Intake.select :close @20:37:37.99 SSV.dial :exhaust @20:37:38.77 Exhaust.select :open @20:37:38.88 SS.seek 0ml @20:37:48.28 Exhaust.select :close @20:37:48.39 SSV.dial CBV @20:37:48.41
CBV.dial SSV @20:37:49.20 Allowing 130s for 25.0ml of remaining 475.0ml @20:37:49.21 Intake.select :open @20:37:51.37 Intake @15.1psia, vacuum from 9.6 to 12.6psi @20:37:51.39 SS.reconfigure :inhaling 0.56ml/s @20:37:51.44 SS.seek 25ml @20:38:28.71 Vacuum too high -- waiting up to 40 seconds @20:38:28.72 SS.reconfigure :exhale @20:38:28.77 SS.hold @20:38:36.76 Intake @14.4psia, vacuum from 8.9 to 11.9psi @20:38:36.77 SS.reconfigure :inhaling 0.51ml/s @20:38:36.82 SS.seek 25ml @20:38:47.73 Stroke completed -- waiting for pressure to equalize ... @20:38:47.74 SS.reconfigure :exhale @20:38:47.78 SS.hold @20:39:02.15 Intake.select :close @20:39:02.31 SSV.dial :exhaust @20:39:03.08 Exhaust.select :open @20:39:03.19 SS.seek 0ml @20:39:12.51 Exhaust.select :close @20:39:12.62 SSV.dial CBV @20:39:12.64
CBV.dial SSV @20:39:13.42 Allowing 130s for 25.0ml of remaining 450.0ml @20:39:13.43 Intake.select :open @20:39:15.59 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:39:15.61 SS.reconfigure :inhaling 0.51ml/s @20:39:15.66 SS.seek 25ml @20:40:04.21 Vacuum too high -- waiting up to 40 seconds @20:40:04.22 SS.reconfigure :exhale @20:40:04.27 SS.hold @20:40:12.07 Intake @14.1psia, vacuum from 8.6 to 11.6psi @20:40:12.09 SS.reconfigure :inhaling 0.46ml/s @20:40:12.14 SS.seek 25ml @20:40:15.51 Stroke completed -- waiting for pressure to equalize ... @20:40:15.52 SS.reconfigure :exhale @20:40:15.57 SS.hold @20:40:28.65 Intake.select :close @20:40:28.80 SSV.dial :exhaust @20:40:29.56 Exhaust.select :open @20:40:29.67 SS.seek 0ml @20:40:39.12 Exhaust.select :close @20:40:39.23 SSV.dial CBV @20:40:39.27
CBV.dial SSV @20:40:40.01 Allowing 130s for 25.0ml of remaining 425.0ml @20:40:40.02 Intake.select :open @20:40:42.18 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:40:42.20 SS.reconfigure :inhaling 0.46ml/s @20:40:42.25 SS.seek 25ml @20:41:37.88 Stroke completed -- waiting for pressure to equalize ... @20:41:37.89 SS.reconfigure :exhale @20:41:37.94 SS.hold @20:41:58.36 Intake.select :close @20:41:58.52 SSV.dial :exhaust @20:41:59.29 Exhaust.select :open @20:41:59.77 SS.seek 0ml @20:42:09.20 Exhaust.select :close @20:42:09.31 SSV.dial CBV @20:42:09.34
CBV.dial SSV @20:42:10.11 Allowing 130s for 25.0ml of remaining 400.0ml @20:42:10.12 Intake.select :open @20:42:12.28 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:42:12.30 SS.reconfigure :inhaling 0.46ml/s @20:42:12.35 SS.seek 25ml @20:42:50.17 Vacuum too high -- waiting up to 40 seconds @20:42:50.19 SS.reconfigure :exhale @20:42:50.24 SS.hold @20:42:57.81 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:42:57.82 SS.reconfigure :inhaling 0.42ml/s @20:42:57.87 SS.seek 25ml @20:43:18.12 Stroke completed -- waiting for pressure to equalize ... @20:43:18.13 SS.reconfigure :exhale @20:43:18.17 SS.hold @20:43:34.79 Intake.select :close @20:43:34.94 SSV.dial :exhaust @20:43:35.73 Exhaust.select :open @20:43:35.84 SS.seek 0ml @20:43:45.18 Exhaust.select :close @20:43:45.29 SSV.dial CBV @20:43:45.31
CBV.dial SSV @20:43:46.10 Allowing 130s for 25.0ml of remaining 375.0ml @20:43:46.11 Intake.select :open @20:43:48.27 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:43:48.29 SS.reconfigure :inhaling 0.42ml/s @20:43:48.34 SS.seek 25ml @20:44:38.92 Vacuum too high -- waiting up to 40 seconds @20:44:38.93 SS.reconfigure :exhale @20:44:38.98 SS.hold @20:44:48.10 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:44:48.12 SS.reconfigure :inhaling 0.38ml/s @20:44:48.17 SS.seek 25ml @20:45:01.65 Stroke completed -- waiting for pressure to equalize ... @20:45:01.66 SS.reconfigure :exhale @20:45:01.70 SS.hold @20:45:17.98 Intake.select :close @20:45:18.13 SSV.dial :exhaust @20:45:18.91 Exhaust.select :open @20:45:19.02 SS.seek 0ml @20:45:28.61 Exhaust.select :close @20:45:28.72 SSV.dial CBV @20:45:28.74
CBV.dial SSV @20:45:29.54 Allowing 130s for 25.0ml of remaining 350.0ml @20:45:29.55 Intake.select :open @20:45:31.71 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:45:31.73 SS.reconfigure :inhaling 0.38ml/s @20:45:31.78 SS.seek 25ml @20:46:40.60 Stroke completed -- waiting for pressure to equalize ... @20:46:40.61 SS.reconfigure :exhale @20:46:40.65 SS.hold @20:46:59.25 Intake.select :close @20:46:59.40 SSV.dial :exhaust @20:47:00.18 Exhaust.select :open @20:47:00.29 SS.seek 0ml @20:47:09.66 Exhaust.select :close @20:47:09.77 SSV.dial CBV @20:47:09.79
CBV.dial SSV @20:47:10.58 Allowing 130s for 25.0ml of remaining 325.0ml @20:47:10.59 Intake.select :open @20:47:12.80 Intake @15.5psia, vacuum from 10.0 to 13.0psi @20:47:12.82 SS.reconfigure :inhaling 0.38ml/s @20:47:12.87 SS.seek 25ml @20:48:08.63 Vacuum too high -- waiting up to 40 seconds @20:48:08.65 SS.reconfigure :exhale @20:48:08.70 SS.hold @20:48:17.64 Intake @15.0psia, vacuum from 9.5 to 12.5psi @20:48:17.66 SS.reconfigure :inhaling 0.33ml/s @20:48:17.71 SS.seek 25ml @20:48:34.25 Stroke completed -- waiting for pressure to equalize ... @20:48:34.26 SS.reconfigure :exhale @20:48:34.31 SS.hold @20:48:50.68 Intake.select :close @20:48:50.84 SSV.dial :exhaust @20:48:51.61 Exhaust.select :open @20:48:51.72 SS.seek 0ml @20:49:01.27 Exhaust.select :close @20:49:01.38 SSV.dial CBV @20:49:01.41
CBV.dial SSV @20:49:02.17 Allowing 130s for 25.0ml of remaining 300.0ml @20:49:02.18 Intake.select :open @20:49:04.34 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:49:04.36 SS.reconfigure :inhaling 0.33ml/s @20:49:04.41 SS.seek 25ml @20:49:50.29 Vacuum too high -- waiting up to 40 seconds @20:49:50.30 SS.reconfigure :exhale @20:49:50.35 SS.hold @20:50:01.62 Intake @15.0psia, vacuum from 9.5 to 12.5psi @20:50:01.63 SS.reconfigure :inhaling 0.30ml/s @20:50:01.68 SS.seek 25ml @20:50:40.76 Stroke completed -- waiting for pressure to equalize ... @20:50:40.77 SS.reconfigure :exhale @20:50:40.82 SS.hold @20:51:12.19 SS.reconfigure :default @20:51:12.24 SS.hold @20:51:12.30 Intake.select :close @20:51:12.45 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 725.0ml @20:51:12.47 SSV.dial :exhaust @20:51:13.23 Exhaust.select :open @20:51:13.34 SS.reconfigure :exhale @20:51:13.39 SS.seek 0ml @20:51:22.84 Exhaust.select :close @20:51:22.95 Sampled 725.0ml @20:51:22.97 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @20:51:22.98 Intake.select :close @20:51:23.04 CSV.dial :waste @20:51:23.18 SSV.dial CBV,avoiding: :exhaust @20:51:23.23 CS.seek 0ml @20:51:23.24
CBV.dial SSV @20:51:23.88 CSV.dial :air @20:51:24.63 CS.seek 10ml @20:51:25.61
CTV.dial :air @20:51:26.38 delay 15 seconds @20:51:41.40 SSV.dial :spit,avoiding: :exhaust @20:51:42.74 CTV.dial CSV,avoiding: :intake @20:51:42.79
SS.seek 0ml @20:51:42.90 SSV.dial CBV,avoiding: :exhaust @20:51:48.61 SSV.dial between CBV and plug @20:51:49.11 CBV.dial between SSV and waste2 @20:51:49.51 CSV.dial between air and CTV @20:51:49.82 CS.seek 8.487ml @20:51:54.21 CTV.dial CSV,avoiding: :air @20:51:54.23
CSV.dial CTV @20:51:54.58 CS.reconfigure :pressurize @20:51:54.63 CS.seek 0ml @20:52:13.87 BubblePoint: Intake @24.2psia, Outlet @0.2psia @20:52:13.88 CBV.dial SSV @20:52:14.22 SS.reconfigure :default @20:52:14.27 SS.seek 10ml @20:52:19.89 delay 15 seconds @20:52:35.02 Evacuated: Intake @23.7psia, Outlet @22.6psia @20:52:35.03 SSV.dial :spit,avoiding: :exhaust @20:52:36.08 SS.reconfigure :exhale @20:52:36.13 SS.seek 0ml @20:52:41.55 SS.reconfigure :fast @20:52:41.60 SS.seek 5ml @20:52:44.19 SSV.dial CBV,avoiding: :exhaust @20:52:44.22
CBV.dial SSV @20:52:45.59 CTV.dial :air,avoiding: :intake @20:52:46.96 CSV.dial CTV @20:52:46.99
SS.seek 10ml @20:52:48.19 CSV.dial :air @20:52:48.75 CS.reconfigure :fast @20:52:48.80 CS.seek 0ml @20:52:49.50
SSV.dial :spit,avoiding: :exhaust @20:52:50.85 SS.reconfigure :exhale @20:52:50.89 SS.seek 0ml @20:52:52.30 CS.stop @20:52:55.90
SS.reconfigure :fast @20:52:55.95 SS.seek 5ml @20:52:58.74 SSV.dial CBV,avoiding: :exhaust @20:52:58.76
CBV.dial SSV @20:53:01.57 SSV.dial :spit,avoiding: :exhaust @20:53:02.89 SS.reconfigure :default @20:53:02.94 SS.seek 0ml @20:53:06.18 SS.stop @20:53:06.23 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @20:53:06.24 Intake.select :close @20:53:06.30 CSV.dial :waste @20:53:06.44 SSV.dial CBV,avoiding: :exhaust @20:53:06.46
CBV.dial SSV @20:53:07.08 CS.seek 0ml @20:53:07.14 CSV.dial :air @20:53:07.84
CTV.dial :air @20:53:07.92 delay 15 seconds @20:53:07.99 CS.seek 10ml @20:53:22.94
SSV.dial :spit,avoiding: :exhaust @20:53:24.27 CTV.dial CSV,avoiding: :intake @20:53:24.30
SS.reconfigure :exhale @20:53:24.40 SS.seek 0ml @20:53:24.90 SSV.dial CBV,avoiding: :exhaust @20:53:31.86 SSV.dial between CBV and plug @20:53:32.36 CBV.dial between SSV and waste2 @20:53:32.75 CSV.dial between air and CTV @20:53:33.20 CS.seek 7.791ml @20:53:39.11 CTV.dial CSV,avoiding: :air @20:53:39.14
CSV.dial CTV @20:53:39.49 CS.reconfigure :pressurize @20:53:39.54 CS.seek 0ml @20:53:57.05 BubblePoint: Intake @24.0psia, Outlet @0.5psia @20:53:57.06 CBV.dial SSV @20:53:57.41 SS.reconfigure :default @20:53:57.46 SS.seek 10ml @20:54:03.06 delay 15 seconds @20:54:18.19 Evacuated: Intake @23.8psia, Outlet @10.9psia @20:54:18.20 SSV.dial :spit,avoiding: :exhaust @20:54:19.25 SS.reconfigure :exhale @20:54:19.30 SS.seek 0ml @20:54:24.37 SS.reconfigure :fast @20:54:24.42 SS.seek 5ml @20:54:27.20 SSV.dial CBV,avoiding: :exhaust @20:54:27.24
CBV.dial SSV @20:54:28.58 CTV.dial :air,avoiding: :intake @20:54:29.94 CSV.dial CTV @20:54:29.99
SS.seek 10ml @20:54:31.54 CSV.dial :air @20:54:31.99 CS.reconfigure :fast @20:54:32.04 CS.seek 0ml @20:54:32.50
SSV.dial :spit,avoiding: :exhaust @20:54:33.85 SS.reconfigure :exhale @20:54:33.90 SS.seek 0ml @20:54:35.58 CS.stop @20:54:39.02
SS.reconfigure :fast @20:54:39.07 SS.seek 5ml @20:54:42.08 SSV.dial CBV,avoiding: :exhaust @20:54:42.10
CBV.dial SSV @20:54:44.46 SSV.dial :spit,avoiding: :exhaust @20:54:45.80 SS.reconfigure :default @20:54:45.84 SS.seek 0ml @20:54:48.89 SS.stop @20:54:48.92 Lysing: Adding 2.0ml AqMeOH @20:54:48.94 CBV.dial between CSV and waste1,avoiding: :DBV @20:54:50.42 CTV.dial CSV @20:54:51.76 CSV.dial CSR @20:54:52.55 Pulling 1ml of CSR.AqMeOH @20:54:52.56 CSR.select :AqMeOH @20:54:52.68 CS.reconfigure :default @20:54:52.73 CS.seek 1ml @20:54:56.17 delay 5 seconds @20:55:01.19 CSR.select :air @20:55:01.36 CS.seek 6ml @20:55:14.95 delay 2 seconds @20:55:16.97 CSV.dial :waste2 @20:55:17.41 CS.reconfigure :fast @20:55:17.46 CS.seek 0ml @20:55:31.83 CSV.dial CSR @20:55:32.28 Pulling 2ml of CSR.AqMeOH @20:55:32.29 CSR.select :AqMeOH @20:55:32.40 CS.reconfigure :default @20:55:32.45 CS.seek 2ml @20:55:38.45 delay 5 seconds @20:55:43.47 CSR.select :air @20:55:43.64 CS.seek 5ml @20:55:52.14 delay 2 seconds @20:55:54.15 CSV.dial CTV @20:55:54.60 CS.seek 1ml @20:56:05.78 Before lysing Collection Puck Heater at 19C/16.4psia @20:56:05.80 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @20:56:05.82 CH.seek 70C,8:00 @20:58:24.16 CH.hold 10:00,70C @20:58:24.36 Begun lysing Collection Puck Heater at 68.1C/22.5psia @21:08:24.37 Ended lysing Collection Puck Heater at 70C/20.4psia @21:08:24.38 CS.seek 0ml @21:08:27.76 CTV.dial between spare and air,avoiding: :intake @21:08:28.82 CSV.dial :air @21:08:29.27 CS.seek 5ml @21:08:42.91 CSV.dial CTV @21:08:43.36 delay 2 seconds @21:08:45.38 CSV.dial :air @21:08:45.83 delay 2 seconds @21:08:47.85 CSV.dial CTV @21:08:48.31 delay 2 seconds @21:08:50.33 CSV.dial :air @21:08:50.79 delay 2 seconds @21:08:52.81 CS.seek 0ml @21:09:06.46 Puck @28.2psia after final lysis pressurization @21:09:06.47 Recovering Lysate into Collection syringe @21:09:06.48 CSV.dial CBV @21:09:07.49 CBV.dial CSV @21:09:07.86 CS.seek 5ml @21:09:21.53 delay 5 seconds @21:09:26.55 CTV.dial :air @21:09:27.08 CS.seek 7ml @21:09:33.00 delay 5 seconds @21:09:38.02 CSV.dial :air2 @21:09:38.46 CS.seek 10ml @21:09:46.94 CSV.dial between air and CTV,avoiding: CBV @21:09:48.30 Lysing Complete @21:09:48.37 Storing sh1 puck in Collection Clamp to tube 2 @21:09:48.54 SC.reconfigure :tight @21:09:48.59 SC.seek 2 @21:09:52.12 SC.configure :limp @21:09:52.33 Elbow.reconfigure :tight @21:09:52.38 Elbow.seek 2 @21:09:54.07 Forearm.reconfigure :tight @21:09:54.11 Forearm.seek 2 + 800 counts @21:09:55.67 SE.reconfigure :pick @21:09:55.73 SE.seek :beamBreak @21:09:58.57 SE.configure :default @21:09:58.73 Elbow.reconfigure :limp @21:09:58.78 Forearm.reconfigure :loose @21:09:58.83 Forearm.seek 2 @21:09:59.21 Elbow.hold @21:09:59.27 Elbow.reconfigure :tight @21:09:59.38 SE.seek 1.09 Pucks below Top Plate @21:09:59.75 Forearm.force -20 @21:09:59.80 Forearm.seek 2 + 320 counts @21:10:00.08 Forearm.reconfigure :tight @21:10:00.13 Forearm.seek 2 + 800 counts @21:10:00.41 SE.seek 2.09 Pucks below Top Plate @21:10:00.94 Forearm.reconfigure :loose @21:10:00.98 Forearm.seek :retract @21:10:01.89 Elbow.seek CC @21:10:03.49 Elbow.reconfigure :loose @21:10:03.60 Forearm.seek CC @21:10:04.54 Hand.close @21:10:05.25 CC.seek :open @21:10:09.45 Forearm.seek :retract @21:10:10.43 Elbow.reconfigure :tight @21:10:10.48 Elbow.seek 2 @21:10:12.20 Elbow.reconfigure :limp @21:10:12.25 Forearm.seek 2 @21:10:13.38 Elbow.hold @21:10:13.46 Elbow.reconfigure :tight @21:10:13.50 Hand.open @21:10:14.75 Forearm.force -20 @21:10:14.86 Forearm.seek 2 + 316 counts @21:10:15.22 Forearm.reconfigure :tight @21:10:15.27 Forearm.seek 2 + 800 counts @21:10:15.64 SE.seek :down @21:10:16.78
Forearm.reconfigure :loose @21:10:16.82 Forearm.seek :retract @21:10:18.19 SE.stop @21:10:18.23
Tranferring DA @21:10:18.24 Beginning samplePassC @21:10:18.25 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:10:18.44 Elbow.seek FlushPuck::Garage @21:10:20.22 Elbow.reconfigure :loose @21:10:20.32 Forearm.seek :garage @21:10:21.37 Hand.close @21:10:22.24 Forearm.seek :retract @21:10:23.28 Elbow.reconfigure :tight @21:10:23.32 Elbow.seek CC @21:10:26.14 Elbow.reconfigure :loose @21:10:26.24 Forearm.reconfigure :tight @21:10:26.29 Forearm.seek CC @21:10:27.56 CC.seek :closed @21:10:31.54 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.04 @21:10:31.60 Hand.open @21:10:32.49 Forearm.reconfigure :loose @21:10:32.54 Forearm.seek :retract @21:10:33.55 PSV.dial PSR @21:10:34.92 Pulling 1.5ml of PSR.flush @21:10:34.93 PSR.select :flush @21:10:35.06 PS.seek 1.5ml @21:10:51.24 delay 5 seconds @21:10:56.26 PSV.dial :waste1 @21:10:56.75 PS.seek 0ml @21:11:12.83 PSV.dial PSR @21:11:13.30 PSR.select :air @21:11:13.42 PS.seek 2.5ml @21:11:39.87 PRV.dial CBV,avoiding: :ab2 @21:11:39.89
CBV.dial PRV @21:11:40.69 PSV.dial :mixloop @21:11:41.72 CTV.dial :air,avoiding: :intake @21:11:41.80 PTV.dial :PRVmixing @21:11:42.53 PS.seek 0ml @21:12:08.86 PSV.dial :air @21:12:09.32 PS.seek 2.5ml @21:12:35.67 PSV.dial :mixloop @21:12:36.13 PS.seek 0ml @21:13:02.71 PSV.dial :air @21:13:03.16 PS.seek 2.5ml @21:13:29.55 PSV.dial :mixloop @21:13:30.01 PS.seek 0ml @21:13:56.33 Garaging FlushPuck in Collection Clamp @21:13:56.40 Elbow.reconfigure :tight @21:13:56.45 Elbow.seek CC @21:13:56.57 Elbow.reconfigure :loose @21:13:56.67 Forearm.seek CC @21:13:57.68 Hand.close @21:13:58.37 CC.seek :open @21:14:02.49 Forearm.seek :retract @21:14:03.46 Elbow.reconfigure :tight @21:14:03.51 Elbow.seek :garage @21:14:06.80 Elbow.reconfigure :loose @21:14:06.90 Forearm.reconfigure :tight @21:14:06.95 Forearm.seek :garage @21:14:08.08 Hand.open @21:14:08.93 Forearm.reconfigure :loose @21:14:08.97 Forearm.seek :retract @21:14:10.16 PSV.dial :mixloop @21:14:10.24 PTV.dial :PRVmixing @21:14:10.25 PRV.dial CBV @21:14:10.29
CBV.dial PRV @21:14:10.42 CTV.dial :air,avoiding: :intake @21:14:10.50 Loading lyfil puck from tube 3 into Collection Clamp @21:14:10.74 SC.reconfigure :tight @21:14:10.79 SC.seek 3 @21:14:14.39 SC.configure :limp @21:14:14.61 Elbow.reconfigure :tight @21:14:14.66 Elbow.seek 3 @21:14:16.55 Forearm.reconfigure :tight @21:14:16.60 Forearm.seek 3 + 800 counts @21:14:17.62 SE.reconfigure :pick @21:14:17.67 SE.seek :beamBreak @21:14:18.99 SE.configure :default @21:14:19.15 Elbow.seek 3 @21:14:19.33 Forearm.seek 3 + 300 counts @21:14:19.61 Forearm.reconfigure :loose @21:14:19.66 Hand.close @21:14:20.38 SE.seek :down @21:14:20.57
Forearm.seek :retract @21:14:21.43 SE.stop @21:14:21.93
Elbow.seek CC @21:14:23.55 Elbow.reconfigure :loose @21:14:23.65 Forearm.reconfigure :tight @21:14:23.70 Forearm.seek CC @21:14:25.02 CC.seek :closed @21:14:29.03 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.17 @21:14:29.04 Hand.open @21:14:29.81 Forearm.reconfigure :loose @21:14:29.86 Forearm.seek :retract @21:14:31.02 CTV.dial CSV,avoiding: :intake @21:14:32.39 CSV.dial CTV @21:14:32.83 PS.seek 2.5ml @21:14:59.20 CS.seek 5ml @21:15:12.81 delay 8 seconds @21:15:20.83 PSV.dial :air @21:15:21.28 delay 2 seconds @21:15:23.30 PSV.dial :waste2 @21:15:24.04 PS.seek 0ml @21:15:50.45 CBV.dial between PRV and waste1 @21:15:50.78 CSV.dial :air @21:15:51.22 CS.seek 10ml @21:16:04.78 CSV.dial CTV @21:16:05.24 CS.seek 0ml @21:16:31.62 CBV.dial :waste1 @21:16:31.93 delay 5 seconds @21:16:36.96 CTV.dial :air,avoiding: :intake @21:16:38.35 Storing lyfil puck in Collection Clamp to tube 2 @21:16:38.52 SC.reconfigure :tight @21:16:38.57 SC.seek 2 @21:16:42.23 SC.configure :limp @21:16:42.45 Elbow.reconfigure :tight @21:16:42.50 Elbow.seek 2 @21:16:44.22 Forearm.reconfigure :tight @21:16:44.27 Forearm.seek 2 + 800 counts @21:16:45.28 SE.reconfigure :pick @21:16:45.34 SE.seek :beamBreak @21:16:48.08 SE.configure :default @21:16:48.24 Elbow.reconfigure :limp @21:16:48.29 Forearm.reconfigure :loose @21:16:48.33 Forearm.seek 2 @21:16:48.70 Elbow.hold @21:16:48.77 Elbow.reconfigure :tight @21:16:48.88 SE.seek 2.1 Pucks below Top Plate @21:16:49.32 Forearm.force -20 @21:16:49.37 Forearm.seek 2 + 318 counts @21:16:49.64 Forearm.reconfigure :tight @21:16:49.69 Forearm.seek 2 + 800 counts @21:16:49.90 SE.seek 3.1 Pucks below Top Plate @21:16:50.43 Forearm.reconfigure :loose @21:16:50.48 Forearm.seek :retract @21:16:51.43 Elbow.seek CC @21:16:53.22 Elbow.reconfigure :loose @21:16:53.32 Forearm.seek CC @21:16:54.28 Hand.close @21:16:54.99 CC.seek :open @21:16:59.67 Forearm.seek :retract @21:17:00.66 Elbow.reconfigure :tight @21:17:00.71 Elbow.seek 2 @21:17:02.37 Elbow.reconfigure :limp @21:17:02.42 Forearm.seek 2 @21:17:03.55 Elbow.hold @21:17:03.62 Elbow.reconfigure :tight @21:17:03.66 Hand.open @21:17:04.49 Forearm.force -20 @21:17:04.54 Forearm.seek 2 + 318 counts @21:17:04.92 Forearm.reconfigure :tight @21:17:04.97 Forearm.seek 2 + 800 counts @21:17:05.31 SE.seek :down @21:17:06.45
Forearm.reconfigure :loose @21:17:06.50 Forearm.seek :retract @21:17:07.70 SE.stop @21:17:07.74
PSV.dial :air @21:17:08.47 PS.seek 2.5ml @21:17:34.86 delay 1 second @21:17:35.87 PSV.dial :mixloop @21:17:36.33 delay 1 second @21:17:37.34 PSV.dial :air @21:17:37.81 delay 1 second @21:17:38.83 PSV.dial :mixloop @21:17:39.28 delay 1 second @21:17:40.30 PSV.dial :air @21:17:40.75 delay 1 second @21:17:41.77 PS.seek 0ml @21:18:08.11 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @21:18:08.29 Elbow.seek FlushPuck::Garage @21:18:10.05 Elbow.reconfigure :loose @21:18:10.15 Forearm.seek :garage @21:18:11.22 Hand.close @21:18:12.55 Forearm.seek :retract @21:18:13.68 Elbow.reconfigure :tight @21:18:13.73 Elbow.seek PC @21:18:15.05 Elbow.reconfigure :loose @21:18:15.15 Forearm.reconfigure :tight @21:18:15.20 Forearm.seek PC @21:18:16.53 PC.seek :closed @21:18:20.95 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.53 @21:18:21.02 Hand.open @21:18:21.80 Forearm.reconfigure :loose @21:18:21.84 Forearm.seek :retract @21:18:22.86 PRV.dial :air @21:18:23.33 PSV.dial :mixloop @21:18:23.97 PS.seek 0.3ml @21:18:27.98 PSV.dial PSR @21:18:29.06 Pulling 0.4ml of PSR.air @21:18:29.07 PSR.select :air @21:18:29.09 PS.seek 0.7ml @21:18:34.08 delay 3 seconds @21:18:37.10 PSR.select :air @21:18:37.12 PS.seek 2.5ml @21:18:56.36 delay 3 seconds @21:18:59.37 PSV.dial :mixloop @21:19:00.42 PTV.dial :mixing @21:19:01.15 PBV.dial :waste2 @21:19:02.13 PS.seek 0ml @21:19:28.51 Garaging FlushPuck in Processing Clamp @21:19:28.59 Elbow.reconfigure :tight @21:19:28.63 Elbow.seek PC @21:19:28.75 Elbow.reconfigure :loose @21:19:29.28 Forearm.seek PC @21:19:30.39 Hand.close @21:19:31.11 PC.seek :open @21:19:35.60 Forearm.seek :retract @21:19:36.58 Elbow.reconfigure :tight @21:19:36.63 Elbow.seek :garage @21:19:38.04 Elbow.reconfigure :loose @21:19:38.14 Forearm.reconfigure :tight @21:19:38.18 Forearm.seek :garage @21:19:39.28 Hand.open @21:19:40.04 Forearm.reconfigure :loose @21:19:40.09 Forearm.seek :retract @21:19:41.20 Loading da puck from tube 3 into Processing Clamp @21:19:41.44 SC.reconfigure :tight @21:19:41.49 SC.seek 3 @21:19:45.12 SC.configure :limp @21:19:45.33 Elbow.reconfigure :tight @21:19:45.38 Elbow.seek 3 @21:19:47.27 Forearm.reconfigure :tight @21:19:47.32 Forearm.seek 3 + 800 counts @21:19:48.34 SE.reconfigure :pick @21:19:48.39 SE.seek :beamBreak @21:19:49.79 SE.configure :default @21:19:49.95 Elbow.seek 3 @21:19:50.20 Forearm.seek 3 + 300 counts @21:19:50.37 Forearm.reconfigure :loose @21:19:50.42 Hand.close @21:19:51.12 SE.seek :down @21:19:51.32
Forearm.seek :retract @21:19:52.32 SE.stop @21:19:52.78
Elbow.seek PC @21:19:55.34 Elbow.reconfigure :loose @21:19:55.44 Forearm.reconfigure :tight @21:19:55.48 Forearm.seek PC @21:19:56.88 PC.seek :closed @21:20:01.30 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.56 @21:20:01.31 Hand.open @21:20:02.17 Forearm.reconfigure :loose @21:20:02.22 Forearm.seek :retract @21:20:03.32 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @21:20:03.34 PH.seek 28C,5:00 @21:20:48.12 PH.hold 2:00:00,28C @21:20:48.17 delay 1:30 @21:22:18.21 PSR.select :air @21:22:18.22 PSV.dial PSR @21:22:19.25 Pulling 0.5ml of PSR.wash PSR.select :wash @21:22:19.37 PS.seek 0.5ml @21:22:25.42 delay 5 seconds @21:22:30.44 PSR.select :air @21:22:30.56 PS.seek 2ml @21:22:46.67 PTV.dial :puck @21:22:47.40 PSV.dial PTV @21:22:48.15 PBV.dial :waste1 @21:22:49.57 PS.seek 1.5ml @21:22:55.51 delay 3 seconds @21:22:58.53 PBV.dial between PSV and waste1 @21:22:58.89 PS.seek 0ml @21:23:15.20 delay 5:00 @21:28:15.22 recoverPSRreagent08 @21:28:15.23 PTV.dial :puck @21:28:15.30 PBV.dial :waste1 @21:28:15.68 delay 30 seconds @21:28:45.70 PBV.dial between PSV and waste1 @21:28:46.07 PSR.select :air @21:28:46.09 PSV.dial PSR @21:28:46.84 PS.reconfigure :fast @21:28:46.89 PS.seek 2.5ml @21:29:10.16 PSV.dial PTV @21:29:10.91 delay 5 seconds @21:29:15.93 PSV.dial PSR @21:29:16.79 delay 5 seconds @21:29:21.81 PSV.dial PTV @21:29:22.55 delay 5 seconds @21:29:27.57 PSV.dial :air @21:29:28.34 PS.seek 0ml @21:29:51.35 PTV.dial :PRVmixing,via: PRV @21:29:52.66 PSV.dial :mixloop @21:29:53.13 PRV.dial :ab1 @21:29:53.89 PS.reconfigure :slow @21:29:54.38 PS.seek 0.15ml @21:29:57.91 delay 2 seconds @21:29:59.93 CBV.dial PRV @21:29:59.97
PRV.dial CBV @21:30:00.52 PS.seek 0.34ml @21:30:05.01 delay 2 seconds @21:30:07.02 CBV.dial between PRV and PSV @21:30:07.34 PRV.dial :air @21:30:07.79 PS.seek 0.9ml @21:30:19.72 PRV.dial :waste2 @21:30:20.47 PS.seek 0.5ml @21:30:29.19 addPMLReagent08 @21:30:29.20 PSV.dial :air @21:30:29.67 PS.reconfigure :default @21:30:29.71 PS.seek 2ml @21:30:45.85 PSV.dial :PML @21:30:46.32 PTV.dial :mixing @21:30:47.05 PBV.dial :waste1 @21:30:47.49 PS.seek 1.85ml @21:30:49.85 PBV.dial between PSV and waste1 @21:30:50.23 PS.seek 0ml @21:31:09.94 delay 2 seconds @21:31:11.96 Beginning samplePassC @21:31:11.97 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:31:12.16 Elbow.reconfigure :tight @21:31:12.21 Elbow.seek FlushPuck::Garage @21:31:13.57 Elbow.reconfigure :loose @21:31:13.67 Forearm.seek :garage @21:31:14.75 Hand.close @21:31:15.59 Forearm.seek :retract @21:31:16.74 Elbow.reconfigure :tight @21:31:16.79 Elbow.seek CC @21:31:19.59 Elbow.reconfigure :loose @21:31:19.69 Forearm.reconfigure :tight @21:31:19.74 Forearm.seek CC @21:31:21.42 CC.seek :closed @21:31:25.40 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 2.01 @21:31:25.46 Hand.open @21:31:26.28 Forearm.reconfigure :loose @21:31:26.33 Forearm.seek :retract @21:31:27.31 PSV.dial PSR @21:31:28.35 Pulling 1.5ml of PSR.flush @21:31:28.36 PSR.select :flush @21:31:28.48 PS.seek 1.5ml @21:31:44.66 delay 5 seconds @21:31:49.68 PSV.dial :waste1 @21:31:50.15 PS.seek 0ml @21:32:06.25 PSV.dial PSR @21:32:06.72 PSR.select :air @21:32:06.84 PS.seek 2.5ml @21:32:33.19 PRV.dial CBV,avoiding: :ab2 @21:32:33.22
CBV.dial PRV @21:32:34.85 PSV.dial :mixloop @21:32:35.88 CTV.dial :air,avoiding: :intake @21:32:35.95 PTV.dial :PRVmixing @21:32:36.68 PS.seek 0ml @21:33:03.00 PSV.dial :air @21:33:03.46 PS.seek 2.5ml @21:33:29.81 PSV.dial :mixloop @21:33:30.29 PS.seek 0ml @21:33:56.66 PSV.dial :air @21:33:57.13 PS.seek 2.5ml @21:34:23.53 PSV.dial :mixloop @21:34:24.01 PS.seek 0ml @21:34:50.35 Garaging FlushPuck in Collection Clamp @21:34:50.42 Elbow.reconfigure :tight @21:34:50.46 Elbow.seek CC @21:34:50.58 Elbow.reconfigure :loose @21:34:50.68 Forearm.seek CC @21:34:52.11 Hand.close @21:34:52.85 CC.seek :open @21:34:56.94 Forearm.seek :retract @21:34:57.92 Elbow.reconfigure :tight @21:34:57.97 Elbow.seek :garage @21:35:00.84 Elbow.reconfigure :loose @21:35:00.94 Forearm.reconfigure :tight @21:35:00.98 Forearm.seek :garage @21:35:02.06 Hand.open @21:35:02.90 Forearm.reconfigure :loose @21:35:02.95 Forearm.seek :retract @21:35:03.95 delayUntil "21:39:11.95" @21:35:03.98 Whole Cell Archive sampling 1000ml @21:35:04.00 Loading wcr puck from tube 3 into Collection Clamp @21:35:04.24 SC.reconfigure :tight @21:35:04.29 SC.seek 3 @21:35:04.38 SC.configure :limp @21:35:04.61 Elbow.reconfigure :tight @21:35:04.66 Elbow.seek 3 @21:35:06.57 Forearm.reconfigure :tight @21:35:06.62 Forearm.seek 3 + 800 counts @21:35:07.75 SE.reconfigure :pick @21:35:07.81 SE.seek :beamBreak @21:35:09.36 SE.configure :default @21:35:09.52 Elbow.seek 3 @21:35:09.79 Forearm.seek 3 + 300 counts @21:35:10.09 Forearm.reconfigure :loose @21:35:10.55 Hand.close @21:35:11.36 SE.seek :down @21:35:11.57 Forearm.seek :retract @21:35:12.61 Elbow.seek CC @21:35:12.68 SE.stop @21:35:14.20 Elbow.reconfigure :loose @21:35:14.30 Forearm.reconfigure :tight @21:35:14.35 Forearm.seek CC @21:35:15.67 CC.seek :closed @21:35:19.65 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.97 @21:35:19.66 Hand.open @21:35:20.41 Forearm.reconfigure :loose @21:35:20.46 Forearm.seek :retract @21:35:21.52 collectSample 1000ml w/bubble point of 35psi @21:35:21.53 Priming 7ml @21:35:21.54 Intake.select :close @21:35:21.55 Exhaust.select :close @21:35:21.56 SS.hold @21:35:21.78 Calibrating pressure sensors @21:35:21.79 CTV.dial :air @21:35:21.87 SSV.dial :spit,avoiding: :exhaust @21:35:21.95 SS.reconfigure :fast @21:35:22.01 SS.seek 5ml @21:35:24.73 SSV.dial CBV @21:35:24.76 CBV.dial SSV @21:35:26.12 delay 3.8 seconds @21:35:30.05 Sensor limits: Intake max 108.9psia; Outlet max 111.8psia @21:35:30.06 SSV.dial :spit @21:35:31.39 SS.seek 0ml @21:35:34.82 SSV.dial CBV @21:35:36.15 CTV.dial :intake @21:35:36.90 SSV.dial CBV @21:35:36.93 CBV.dial SSV @21:35:37.08 Allowing 40s for 7.0ml of remaining 7.0ml @21:35:37.09 Intake.select :open @21:35:39.26 Intake @14.9psia, vacuum from 9.4 to 12.4psi @21:35:39.28 SS.reconfigure :inhaling 2.17ml/s @21:35:39.33 SS.seek 7ml @21:35:44.91 Stroke completed -- waiting for pressure to equalize ... @21:35:44.93 SS.reconfigure :exhale @21:35:44.97 SS.hold @21:35:56.13 Intake.select :close @21:35:56.29 SSV.dial :exhaust @21:35:57.07 Exhaust.select :open @21:35:57.18 SS.seek 0ml @21:36:02.02 Exhaust.select :close @21:36:02.20 Sampling 1000.0ml @21:36:02.21 Intake.select :close @21:36:02.22 Exhaust.select :close @21:36:02.24 SS.reconfigure :default @21:36:02.28 SS.hold @21:36:02.49 CTV.dial :intake @21:36:02.51 SSV.dial CBV @21:36:02.55 CBV.dial SSV @21:36:03.31 Allowing 130s for 25.0ml of remaining 1000.0ml @21:36:03.32 Intake.select :open @21:36:05.48 Intake @14.1psia, vacuum from 8.6 to 11.6psi @21:36:05.50 SS.reconfigure :inhaling 2.17ml/s @21:36:05.55 SS.seek 25ml @21:36:12.42 Vacuum too high -- waiting up to 40 seconds @21:36:12.44 SS.reconfigure :exhale @21:36:12.49 SS.hold @21:36:24.06 Intake @13.3psia, vacuum from 7.8 to 10.8psi @21:36:24.08 SS.reconfigure :inhaling 1.95ml/s @21:36:24.13 SS.seek 25ml @21:36:28.58 Vacuum too high -- waiting up to 40 seconds @21:36:29.02 SS.reconfigure :exhale @21:36:29.10 SS.hold @21:36:38.99 Intake @13.4psia, vacuum from 7.9 to 10.9psi @21:36:39.01 SS.reconfigure :inhaling 1.77ml/s @21:36:39.06 SS.seek 25ml @21:36:44.24 Vacuum too high -- waiting up to 40 seconds @21:36:44.26 SS.reconfigure :exhale @21:36:44.31 SS.hold @21:36:52.20 Intake @13.3psia, vacuum from 7.8 to 10.8psi @21:36:52.22 SS.reconfigure :inhaling 1.59ml/s @21:36:52.27 SS.seek 25ml @21:36:52.33 Stroke completed -- waiting for pressure to equalize ... @21:36:52.35 SS.reconfigure :exhale @21:36:52.39 SS.hold @21:36:57.16 Intake.select :close @21:36:57.32 SSV.dial :exhaust @21:36:58.10 Exhaust.select :open @21:36:58.21 SS.seek 0ml @21:37:07.07 Exhaust.select :close @21:37:07.18 SSV.dial CBV @21:37:07.22 CBV.dial SSV @21:37:07.99 Allowing 130s for 25.0ml of remaining 975.0ml @21:37:08.01 Intake.select :open @21:37:10.17 Intake @14.0psia, vacuum from 8.5 to 11.5psi @21:37:10.19 SS.reconfigure :inhaling 1.59ml/s @21:37:10.70 SS.seek 25ml @21:37:17.79 Vacuum too high -- waiting up to 40 seconds @21:37:17.81 SS.reconfigure :exhale @21:37:17.86 SS.hold @21:37:23.72 Intake @13.2psia, vacuum from 7.7 to 10.7psi @21:37:23.73 SS.reconfigure :inhaling 1.43ml/s @21:37:23.78 SS.seek 25ml @21:37:28.20 Vacuum too high -- waiting up to 40 seconds @21:37:28.22 SS.reconfigure :exhale @21:37:28.27 SS.hold @21:37:34.96 SS.reconfigure :inhaling 1.29ml/s @21:37:35.01 SS.seek 25ml @21:37:40.29 Vacuum too high -- waiting up to 40 seconds @21:37:40.31 SS.reconfigure :exhale @21:37:40.36 SS.hold @21:37:48.00 Intake @13.4psia, vacuum from 7.9 to 10.9psi @21:37:48.02 SS.reconfigure :inhaling 1.16ml/s @21:37:48.07 SS.seek 25ml @21:37:54.80 Stroke completed -- waiting for pressure to equalize ... @21:37:54.81 SS.reconfigure :exhale @21:37:54.86 SS.hold @21:38:03.88 Intake.select :close @21:38:04.04 SSV.dial :exhaust @21:38:04.80 Exhaust.select :open @21:38:04.92 SS.seek 0ml @21:38:14.27 Exhaust.select :close @21:38:14.38 SSV.dial CBV @21:38:14.41 CBV.dial SSV @21:38:15.19 Allowing 130s for 25.0ml of remaining 950.0ml @21:38:15.21 Intake.select :open @21:38:17.38 Intake @14.3psia, vacuum from 8.8 to 11.8psi @21:38:17.39 SS.reconfigure :inhaling 1.16ml/s @21:38:17.44 SS.seek 25ml @21:38:27.54 Vacuum too high -- waiting up to 40 seconds @21:38:27.56 SS.reconfigure :exhale @21:38:27.61 SS.hold @21:38:33.39 Intake @12.9psia, vacuum from 7.4 to 10.4psi @21:38:33.41 SS.reconfigure :inhaling 1.04ml/s @21:38:33.46 SS.seek 25ml @21:38:40.48 Vacuum too high -- waiting up to 40 seconds @21:38:40.50 SS.reconfigure :exhale @21:38:40.55 SS.hold @21:38:49.25 SS.reconfigure :inhaling 0.94ml/s @21:38:49.30 SS.seek 25ml @21:38:57.48 Vacuum too high -- waiting up to 40 seconds @21:38:57.49 SS.reconfigure :exhale @21:38:57.55 SS.hold @21:39:06.69 Intake @12.5psia, vacuum from 7.0 to 10.0psi @21:39:06.71 SS.reconfigure :inhaling 0.85ml/s @21:39:06.76 SS.seek 25ml @21:39:09.27 Stroke completed -- waiting for pressure to equalize ... @21:39:09.28 SS.reconfigure :exhale @21:39:09.33 SS.hold @21:39:11.96 recoverPRVreagent08 @21:39:11.97 PTV.dial :mixing @21:39:12.71 PBV.dial :waste1 @21:39:13.11 delay 30 seconds @21:39:18.43 Intake.select :close @21:39:18.60 SSV.dial :exhaust @21:39:19.38 Exhaust.select :open @21:39:19.49 SS.seek 0ml @21:39:28.89 Exhaust.select :close @21:39:29.01 SSV.dial CBV @21:39:29.03 CBV.dial SSV @21:39:29.82 Allowing 130s for 25.0ml of remaining 925.0ml @21:39:29.83 Intake.select :open @21:39:31.99 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:39:32.01 SS.reconfigure :inhaling 0.85ml/s @21:39:32.06 SS.seek 25ml @21:39:43.13 PBV.dial between PSV and waste1 @21:39:43.53 PSV.dial :air @21:39:44.00 PS.reconfigure :fast @21:39:44.05 PS.seek 2.5ml @21:39:45.98 Vacuum too high -- waiting up to 40 seconds @21:39:45.99 SS.reconfigure :exhale @21:39:46.05 SS.hold @21:39:54.74 Intake @13.2psia, vacuum from 7.7 to 10.7psi @21:39:54.76 SS.reconfigure :inhaling 0.77ml/s @21:39:54.81 SS.seek 25ml @21:40:03.23 Vacuum too high -- waiting up to 40 seconds @21:40:03.24 SS.reconfigure :exhale @21:40:03.30 SS.hold @21:40:07.53 PSV.dial :mixloop @21:40:08.00 delay 2 seconds @21:40:10.01 PSV.dial :air @21:40:10.48 delay 2 seconds @21:40:12.50 PSV.dial :mixloop @21:40:12.97 delay 2 seconds @21:40:13.19 Intake @13.1psia, vacuum from 7.6 to 10.6psi @21:40:13.21 SS.reconfigure :inhaling 0.70ml/s @21:40:13.26 SS.seek 25ml @21:40:14.98 PSV.dial :air @21:40:15.46 PS.seek 0ml @21:40:25.62 Stroke completed -- waiting for pressure to equalize ... @21:40:25.63 SS.reconfigure :exhale @21:40:25.68 SS.hold @21:40:39.12 washCycleDA #1 of 2 @21:40:39.13 PSR.select :air @21:40:39.15 PSV.dial PSR @21:40:40.48 Pulling 0.5ml of PSR.wash @21:40:40.49 PSR.select :wash @21:40:40.61 PS.reconfigure :default @21:40:40.66 PS.seek 0.5ml @21:40:41.98 Intake.select :close @21:40:42.14 SSV.dial :exhaust @21:40:42.92 Exhaust.select :open @21:40:43.04 SS.seek 0ml @21:40:46.56 delay 5 seconds @21:40:51.58 PSR.select :air @21:40:52.16 PS.seek 2ml @21:40:52.42 Exhaust.select :close @21:40:52.54 SSV.dial CBV @21:40:52.57 CBV.dial SSV @21:40:53.36 Allowing 130s for 25.0ml of remaining 900.0ml @21:40:53.38 Intake.select :open @21:40:55.54 Intake @14.5psia, vacuum from 9.0 to 12.0psi @21:40:55.56 SS.reconfigure :inhaling 0.70ml/s @21:40:55.61 SS.seek 25ml @21:41:06.60 Vacuum too high -- waiting up to 40 seconds @21:41:06.62 SS.reconfigure :exhale @21:41:06.67 SS.hold @21:41:08.26 PTV.dial :puck @21:41:09.01 PSV.dial PTV @21:41:09.78 PBV.dial :waste1 @21:41:10.23 PS.seek 1.5ml @21:41:16.53 delay 3 seconds @21:41:19.99 PBV.dial between PSV and waste1 @21:41:20.41 PS.seek 0ml @21:41:25.31 Intake @14.0psia, vacuum from 8.5 to 11.5psi @21:41:25.33 SS.reconfigure :inhaling 0.62ml/s @21:41:25.38 SS.seek 25ml @21:41:30.98 Vacuum too high -- waiting up to 40 seconds @21:41:30.99 SS.reconfigure :exhale @21:41:31.05 SS.hold @21:41:36.57 delay 2:00 @21:41:51.62 Intake @14.1psia, vacuum from 8.6 to 11.6psi @21:41:51.64 SS.reconfigure :inhaling 0.56ml/s @21:41:51.69 SS.seek 25ml @21:41:59.17 Vacuum too high -- waiting up to 40 seconds @21:41:59.18 SS.reconfigure :exhale @21:41:59.24 SS.hold @21:42:23.92 Intake @14.3psia, vacuum from 8.8 to 11.8psi @21:42:23.94 SS.reconfigure :inhaling 0.51ml/s @21:42:23.99 SS.seek 25ml @21:42:32.88 Vacuum too high -- waiting up to 40 seconds @21:42:32.90 SS.reconfigure :exhale @21:42:32.95 SS.hold @21:43:01.70 Intake @14.3psia, vacuum from 8.8 to 11.8psi @21:43:01.72 SS.reconfigure :inhaling 0.46ml/s @21:43:01.77 SS.seek 25ml @21:43:03.39 SS.reconfigure :default @21:43:03.45 SS.hold @21:43:03.61 Intake.select :close @21:43:03.77 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 118.7ml @21:43:03.79 SSV.dial :exhaust @21:43:04.56 Exhaust.select :open @21:43:04.68 SS.reconfigure :exhale @21:43:04.73 SS.seek 0ml @21:43:12.56 Exhaust.select :close @21:43:12.67 Sampled 118.7ml @21:43:12.69 Drying puck before fixing for 4:00 @21:43:12.70 CBV.dial :waste2b @21:43:13.44 delay 1:00 @21:43:36.59 recoverPSRreagent08 @21:43:36.60 PTV.dial :puck @21:43:36.67 PBV.dial :waste1 @21:43:37.04 delay 30 seconds @21:44:07.07 PBV.dial between PSV and waste1 @21:44:07.45 PSR.select :air @21:44:07.47 PSV.dial PSR @21:44:08.22 PS.reconfigure :fast @21:44:08.27 PS.seek 2.5ml @21:44:13.46 CSV.dial :air @21:44:13.92 CS.reconfigure :fast @21:44:13.97 CS.seek 10ml @21:44:31.77 PSV.dial PTV @21:44:32.53 delay 5 seconds @21:44:37.55 PSV.dial PSR @21:44:37.72 Pumping wcr puck from tube 3 up to 35psi... @21:44:37.74 CBV.dial SSV @21:44:38.29 delay 5 seconds @21:44:39.01 CBV.dial between CSV and waste2b @21:44:39.99 CSV.dial between air and CTV @21:44:40.31 CS.seek 7.237ml @21:44:43.31 PSV.dial PTV @21:44:44.06 delay 5 seconds @21:44:47.44 CTV.dial CSV,avoiding: :air @21:44:47.47 CSV.dial CTV @21:44:48.73 CS.reconfigure :pressurize @21:44:48.80 CS.seek 0ml @21:44:49.07 PSV.dial :air @21:44:49.84 PS.seek 0ml @21:45:07.25 CBV.dial :waste2b @21:45:07.60 CS.reconfigure :default @21:45:07.65 CS.seek 0ml @21:45:09.03 delay 1:00 @21:45:13.40 washCycleDA #2 of 2 @21:45:13.41 PSR.select :air @21:45:13.43 PSV.dial PSR @21:45:14.74 Pulling 0.5ml of PSR.wash @21:45:14.75 PSR.select :wash @21:45:14.87 PS.reconfigure :default @21:45:14.92 PS.seek 0.5ml @21:45:20.90 delay 5 seconds @21:45:25.92 PSR.select :air @21:45:26.04 PS.seek 2ml @21:45:42.15 PTV.dial :puck @21:45:42.23 PSV.dial PTV @21:45:42.96 PBV.dial :waste1 @21:45:43.40 PS.seek 1.5ml @21:45:49.30 delay 3 seconds @21:45:52.32 PBV.dial between PSV and waste1 @21:45:52.70 PS.seek 0ml @21:46:08.96 delay 2:00 @21:46:09.05 CTV.dial :air,avoiding: :intake @21:46:10.41 Fix #1 of 1 (with RNAlater) for 4:00 @21:46:10.43 CSV.dial CSR @21:46:10.86 Pulling 2ml of CSR.RNAlater @21:46:10.87 CSR.select :RNAlater @21:46:11.00 CS.seek 2ml @21:46:17.00 delay 5 seconds @21:46:22.02 CSR.select :air @21:46:22.14 CS.reconfigure :fast @21:46:22.19 CS.seek 10ml @21:46:41.35 Pumping wcr puck from tube 3 up to 35psi... @21:46:41.37 CBV.dial SSV @21:46:42.60 CBV.dial between CSV and waste2b @21:46:43.56 CSV.dial between air and CTV @21:46:44.17 CS.seek 7.075ml @21:46:51.76 CTV.dial CSV,avoiding: :air @21:46:51.78 CSV.dial CTV @21:46:53.18 CS.reconfigure :pressurize @21:46:53.23 CS.seek 0ml @21:47:08.84 delay 4:00 @21:48:08.98 recoverPSRreagent08 @21:48:08.99 PTV.dial :puck @21:48:09.54 PBV.dial :waste1 @21:48:09.91 delay 30 seconds @21:48:39.94 PBV.dial between PSV and waste1 @21:48:40.32 PSR.select :air @21:48:40.34 PSV.dial PSR @21:48:41.09 PS.reconfigure :fast @21:48:41.14 PS.seek 2.5ml @21:49:04.68 PSV.dial PTV @21:49:05.43 delay 5 seconds @21:49:10.45 PSV.dial PSR @21:49:11.19 delay 5 seconds @21:49:16.21 PSV.dial PTV @21:49:16.96 delay 5 seconds @21:49:21.98 PSV.dial :air @21:49:22.75 PS.seek 0ml @21:49:45.96 getPMLReagent08 @21:49:45.97 PRV.dial :air @21:49:46.41 delay 5 seconds @21:49:51.43 PTV.dial :PRVmixing @21:49:52.76 delay 5 seconds @21:49:57.78 PRV.dial :ab2 @21:49:58.22 PTV.dial :PRVmixing @21:49:58.30 PSV.dial :mixloop @21:49:58.77 PS.reconfigure :default @21:49:58.82 PS.seek 0.3ml @21:50:02.77 delay 2 seconds @21:50:04.80 PRV.dial :air @21:50:05.30 PS.seek 0.55ml @21:50:08.65 addPMLReagent08 @21:50:08.66 PSV.dial :air @21:50:09.14 PS.seek 2ml @21:50:24.77 PSV.dial :PML @21:50:25.24 PTV.dial :mixing @21:50:25.97 PBV.dial :waste1 @21:50:26.41 PS.seek 1.85ml @21:50:28.74 PBV.dial between PSV and waste1 @21:50:29.12 PS.seek 0ml @21:50:48.89 delay 2 seconds @21:50:50.91 delay 8:00 @21:51:08.86 CBV.dial :waste2b @21:51:09.19 CS.reconfigure :default @21:51:09.24 CS.seek 0ml @21:51:12.97 delay 1:00 @21:52:13.00 CTV.dial :air,avoiding: :intake @21:58:50.94 recoverPRVreagent08 @21:58:50.95 PTV.dial :mixing @21:58:51.12 PBV.dial :waste1 @21:58:51.51 delay 30 seconds @21:59:21.54 PBV.dial between PSV and waste1 @21:59:21.92 PSV.dial :air @21:59:22.39 PS.reconfigure :fast @21:59:22.44 PS.seek 2.5ml @21:59:45.95 PSV.dial :mixloop @21:59:46.42 delay 2 seconds @21:59:48.44 PSV.dial :air @21:59:48.90 delay 2 seconds @21:59:50.92 PSV.dial :mixloop @21:59:51.46 delay 2 seconds @21:59:53.48 PSV.dial :air @21:59:53.96 PS.seek 0ml @22:00:17.08 washCycleDA #1 of 3 @22:00:17.09 PSR.select :air @22:00:17.10 PSV.dial PSR @22:00:18.42 Pulling 0.5ml of PSR.wash @22:00:18.43 PSR.select :wash @22:00:18.55 PS.reconfigure :default @22:00:18.60 PS.seek 0.5ml @22:00:24.57 delay 5 seconds @22:00:29.59 PSR.select :air @22:00:29.72 PS.seek 2ml @22:00:46.20 PTV.dial :puck @22:00:46.95 PSV.dial PTV @22:00:47.68 PBV.dial :waste1 @22:00:48.12 PS.seek 1.5ml @22:00:54.04 delay 3 seconds @22:00:57.06 PBV.dial between PSV and waste1 @22:00:57.44 PS.seek 0ml @22:01:13.61 delay 2:00 @22:03:13.64 recoverPSRreagent08 @22:03:13.65 PTV.dial :puck @22:03:13.71 PBV.dial :waste1 @22:03:14.09 delay 30 seconds @22:03:44.11 PBV.dial between PSV and waste1 @22:03:44.49 PSR.select :air @22:03:44.51 PSV.dial PSR @22:03:45.27 PS.reconfigure :fast @22:03:45.31 PS.seek 2.5ml @22:04:08.70 PSV.dial PTV @22:04:09.45 delay 5 seconds @22:04:14.47 PSV.dial PSR @22:04:15.20 delay 5 seconds @22:04:20.22 PSV.dial PTV @22:04:20.96 delay 5 seconds @22:04:25.98 PSV.dial :air @22:04:26.75 PS.seek 0ml @22:04:49.92 washCycleDA #2 of 3 @22:04:49.93 PSR.select :air @22:04:49.94 PSV.dial PSR @22:04:51.26 Pulling 0.5ml of PSR.wash @22:04:51.27 PSR.select :wash @22:04:51.39 PS.reconfigure :default @22:04:51.44 PS.seek 0.5ml @22:04:57.35 delay 5 seconds @22:05:02.37 PSR.select :air @22:05:02.49 PS.seek 2ml @22:05:18.63 PTV.dial :puck @22:05:18.71 PSV.dial PTV @22:05:19.45 PBV.dial :waste1 @22:05:19.88 PS.seek 1.5ml @22:05:25.88 delay 3 seconds @22:05:28.90 PBV.dial between PSV and waste1 @22:05:29.28 PS.seek 0ml @22:05:45.42 delay 2:00 @22:07:45.44 recoverPSRreagent08 @22:07:45.45 PTV.dial :puck @22:07:45.52 PBV.dial :waste1 @22:07:45.90 delay 30 seconds @22:08:15.92 PBV.dial between PSV and waste1 @22:08:16.30 PSR.select :air @22:08:16.32 PSV.dial PSR @22:08:17.56 PS.reconfigure :fast @22:08:17.61 PS.seek 2.5ml @22:08:40.85 PSV.dial PTV @22:08:41.60 delay 5 seconds @22:08:46.62 PSV.dial PSR @22:08:47.38 delay 5 seconds @22:08:52.40 PSV.dial PTV @22:08:53.15 delay 5 seconds @22:08:58.17 PSV.dial :air @22:08:58.93 PS.seek 0ml @22:09:22.04 washCycleDA #3 of 3 @22:09:22.05 PSR.select :air @22:09:22.07 PSV.dial PSR @22:09:23.38 Pulling 0.5ml of PSR.wash @22:09:23.39 PSR.select :wash @22:09:23.51 PS.reconfigure :default @22:09:23.56 PS.seek 0.5ml @22:09:29.50 delay 5 seconds @22:09:34.52 PSR.select :air @22:09:34.65 PS.seek 2ml @22:09:50.85 PTV.dial :puck @22:09:50.93 PSV.dial PTV @22:09:51.66 PBV.dial :waste1 @22:09:52.10 PS.seek 1.5ml @22:09:58.00 delay 3 seconds @22:10:01.02 PBV.dial between PSV and waste1 @22:10:01.40 PS.seek 0ml @22:10:17.60 delay 2:00 @22:12:17.63 recoverPSRreagent08 @22:12:17.64 PTV.dial :puck @22:12:17.71 PBV.dial :waste1 @22:12:18.08 delay 30 seconds @22:12:48.11 PBV.dial between PSV and waste1 @22:12:48.49 PSR.select :air @22:12:48.51 PSV.dial PSR @22:12:49.37 PS.reconfigure :fast @22:12:49.42 PS.seek 2.5ml @22:13:12.78 PSV.dial PTV @22:13:13.52 delay 5 seconds @22:13:18.54 PSV.dial PSR @22:13:19.27 delay 5 seconds @22:13:24.29 PSV.dial PTV @22:13:25.03 delay 5 seconds @22:13:30.05 PSV.dial :air @22:13:30.82 PS.seek 0ml @22:13:53.94 Gate.power :camera,:ON @22:13:53.95 Mixing DA Substrates @22:13:53.96 PSR.select :air @22:13:53.98 PSV.dial PSR @22:13:55.37 Pulling 0.25ml of PSR.sub1 @22:13:55.40 PSR.select :sub1 @22:13:55.60 PS.reconfigure :default @22:13:55.72 PS.seek 0.25ml @22:13:59.33 delay 5 seconds @22:14:04.35 Pulling 0.25ml of PSR.sub2 @22:14:04.36 PSR.select :sub2 @22:14:04.48 PS.seek 0.5ml @22:14:07.94 delay 5 seconds @22:14:12.96 PSR.select :air @22:14:13.07 PS.seek 2ml @22:14:29.19 PTV.dial :puck @22:14:29.27 PSV.dial PTV @22:14:30.00 PBV.dial :waste1 @22:14:30.95 PS.seek 1.5ml @22:14:37.09 delay 3 seconds @22:14:40.11 PBV.dial between PSV and waste1 @22:14:40.51 PS.seek 0ml @22:14:56.59 delay 10 seconds @22:15:06.62 recoverPSRreagent08 @22:15:06.63 PTV.dial :puck @22:15:06.70 PBV.dial :waste1 @22:15:07.10 delay 30 seconds @22:15:37.12 PBV.dial between PSV and waste1 @22:15:37.52 PSR.select :air @22:15:37.53 PSV.dial PSR @22:15:38.29 PS.reconfigure :fast @22:15:38.34 PS.seek 2.5ml @22:16:01.87 PSV.dial PTV @22:16:02.63 delay 5 seconds @22:16:07.65 PSV.dial PSR @22:16:08.41 delay 5 seconds @22:16:13.43 PSV.dial PTV @22:16:14.17 delay 5 seconds @22:16:19.19 PSV.dial :air @22:16:19.96 PS.seek 0ml @22:16:43.50 Additional air clear @22:16:43.51 PSR.select :air @22:16:43.53 PSV.dial PSR @22:16:44.86 Pulling 2ml of PSR.air @22:16:44.87 PSR.select :air @22:16:44.89 PS.reconfigure :default @22:16:44.94 PS.seek 2ml @22:17:06.19 delay 5 seconds @22:17:11.21 PTV.dial :puck @22:17:11.30 PSV.dial PTV @22:17:12.03 PBV.dial :waste1 @22:17:12.48 PS.seek 1.5ml @22:17:18.34 delay 3 seconds @22:17:21.36 PBV.dial between PSV and waste1 @22:17:21.75 delay 1 second @22:17:22.77 PSV.dial :waste1 @22:17:23.81 PS.seek 0ml @22:17:40.02 Processing Puck Heater at 27.9C before imaging @22:17:40.03 PH.coast @22:17:40.14 Imaging da puck in Processing Clamp while storing it to tube 2 @22:17:40.32 SC.reconfigure :tight @22:17:40.37 SC.seek 2 @22:17:44.01 SC.configure :limp @22:17:44.23 Elbow.reconfigure :tight @22:17:44.28 Elbow.seek 2 @22:17:46.01 Forearm.reconfigure :tight @22:17:46.06 Forearm.seek 2 + 800 counts @22:17:47.11 SE.reconfigure :pick @22:17:47.16 SE.seek :beamBreak @22:17:50.31 SE.configure :default @22:17:50.53 Elbow.reconfigure :limp @22:17:50.59 Forearm.reconfigure :loose @22:17:50.64 Forearm.seek 2 @22:17:51.05 Elbow.hold @22:17:51.12 Elbow.reconfigure :tight @22:17:51.22 SE.seek 3.1 Pucks below Top Plate @22:17:51.69 Forearm.force -20 @22:17:51.73 Forearm.seek 2 + 315 counts @22:17:52.15 Forearm.reconfigure :tight @22:17:52.20 Forearm.seek 2 + 800 counts @22:17:52.55 SE.seek 4.1 Pucks below Top Plate @22:17:53.12 Forearm.reconfigure :loose @22:17:53.17 Forearm.seek :retract @22:17:54.11 Elbow.seek PC @22:17:56.68 Elbow.reconfigure :loose @22:17:56.78 Forearm.seek PC @22:17:57.89 Hand.close @22:17:58.67 PC.seek :open @22:18:03.23 Forearm.seek :retract @22:18:04.25 Elbow.reconfigure :tight @22:18:04.30 Elbow.seek 2 @22:18:06.91 Forearm.reconfigure :tight @22:18:06.96 Forearm.seek 2 + 800 counts @22:18:08.01 Hand.open @22:18:08.86 SE.seek 3.85 Pucks below Top Plate @22:18:09.11 SE.reconfigure :seal @22:18:09.16 SE.seek 3.2 Pucks below Top Plate @22:18:09.73 Lights, camera, ACTION! @22:18:09.77 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da25oct0822h725ml.tif @22:18:29.22 Waiting >20 seconds for da to relinquish FlushPuck @22:18:35.57 SX HX9/16 (USB:06): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 22.26 seconds /var/log/esp/da25oct0822h725ml.tif: TIFF Upload Complete @22:18:35.60 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da25oct0822h725ml15s.tif @22:18:51.24 SX HX9/16 (USB:06): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da25oct0822h725ml15s.tif: TIFF Upload Complete @22:18:51.27 PuckCamera: snap -bin=2,2 21.0 /var/log/esp/midres/da25oct0822h725ml21s.tif @22:19:12.92 SX HX9/16 (USB:06): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21 seconds /var/log/esp/midres/da25oct0822h725ml21s.tif: TIFF Upload Complete @22:19:12.94 Gate.power :camera,:OFF @22:19:12.96 DAfans.email "22.26 sec auto-exposure",Subject: "da25oct0822h725ml images!" @22:19:13.99 Elbow.seek 2 @22:19:14.25 Forearm.seek 2 + 800 counts @22:19:14.40 SE.reconfigure :gently @22:19:14.46 SE.seek 3.91 Pucks below Top Plate @22:19:15.20 Elbow.reconfigure :limp @22:19:15.26 Forearm.reconfigure :loose @22:19:15.31 Forearm.seek 2 @22:19:15.73 Elbow.hold @22:19:15.80 Elbow.reconfigure :tight @22:19:15.85 SE.reconfigure :default @22:19:15.90 SE.seek 4.1 Pucks below Top Plate @22:19:16.49 Forearm.force -20 @22:19:16.67 Forearm.seek 2 + 312 counts @22:19:16.97 Forearm.reconfigure :tight @22:19:17.03 Forearm.seek 2 + 800 counts @22:19:17.45 SE.seek :down @22:19:18.73 Forearm.reconfigure :loose @22:19:18.83 Forearm.seek :retract @22:19:19.87 SE.stop @22:19:20.11 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @22:19:20.68 Elbow.seek FlushPuck::Garage @22:19:22.66 Elbow.reconfigure :loose @22:19:22.88 Forearm.seek :garage @22:19:24.00 Hand.close @22:19:25.07 Forearm.seek :retract @22:19:26.20 Elbow.reconfigure :tight @22:19:26.30 Elbow.seek PC @22:19:28.77 Elbow.reconfigure :loose @22:19:28.99 Forearm.reconfigure :tight @22:19:29.09 Forearm.seek PC @22:19:30.76 PC.seek :closed @22:19:35.39 Processing Clamp onSpring/offSpring for 12/26 samples, on/off torque 1.56 @22:19:35.56 Hand.open @22:19:36.45 Forearm.reconfigure :loose @22:19:36.54 Forearm.seek :retract @22:19:37.67 PSR.select :air @22:19:37.73 Storing wcr puck in Collection Clamp to tube 2 @22:19:37.78 PSV.dial PSR @22:19:38.40 Pulling 0.5ml of PSR.flush @22:19:38.47 PSR.select :flush @22:19:38.91 PS.seek 0.5ml @22:19:39.05 Elbow.reconfigure :tight @22:19:39.22 Elbow.seek 2 @22:19:42.05 Forearm.reconfigure :tight @22:19:42.14 Forearm.seek 2 + 800 counts @22:19:43.39 SE.reconfigure :pick @22:19:43.51 SE.seek :beamBreak @22:19:44.92 delay 5 seconds @22:19:46.29 SE.configure :default @22:19:46.61 Elbow.reconfigure :limp @22:19:47.68 Forearm.reconfigure :loose @22:19:47.81 Forearm.seek 2 @22:19:48.29 Elbow.hold @22:19:48.42 Elbow.reconfigure :tight @22:19:48.64 SE.seek 4.09 Pucks below Top Plate @22:19:49.24 Forearm.force -20 @22:19:49.33 Forearm.seek 2 + 312 counts @22:19:49.88 Forearm.reconfigure :tight @22:19:49.99 Forearm.seek 2 + 800 counts @22:19:50.05 PSR.select :air @22:19:50.27 PS.seek 2.5ml @22:19:50.51 SE.seek 5.09 Pucks below Top Plate @22:19:51.36 Forearm.reconfigure :loose @22:19:51.46 Forearm.seek :retract @22:19:52.20 upsync @22:19:52.47 Elbow.seek CC @22:19:54.07 Elbow.reconfigure :loose @22:19:54.17 Forearm.seek CC @22:19:55.15 Hand.close @22:19:55.92 CC.seek :open @22:20:00.11 Forearm.seek :retract @22:20:01.13 Elbow.reconfigure :tight @22:20:01.18 Elbow.seek 2 @22:20:02.89 Elbow.reconfigure :limp @22:20:02.95 Forearm.seek 2 @22:20:04.09 Elbow.hold @22:20:04.16 Elbow.reconfigure :tight @22:20:04.21 Hand.open @22:20:05.11 Forearm.force -20 @22:20:05.16 Forearm.seek 2 + 318 counts @22:20:05.45 Forearm.reconfigure :tight @22:20:05.50 Forearm.seek 2 + 800 counts @22:20:05.90 SE.seek :down @22:20:07.04 Forearm.reconfigure :loose @22:20:07.09 Forearm.seek :retract @22:20:08.13 SE.stop @22:20:08.17
Starting finalPurge @22:20:11.59 delay 1 second @22:20:12.61 PRV.dial :waste2,avoiding: :ab2 @22:20:14.48 PTV.dial :PRVmixing @22:20:15.78 PSV.dial :mixloop @22:20:16.82 PS.seek :empty @22:20:28.06
Waiting >20 seconds for psFlush to relinquish FlushPuck @22:20:43.24 PSR.select :air @22:20:43.26 PSV.dial PSR @22:20:44.31 Pulling 0.5ml of PSR.flush @22:20:44.32 PSR.select :flush @22:20:44.44 PS.seek 0.5ml @22:20:50.43 delay 5 seconds @22:20:55.45 PSR.select :air @22:20:55.57 PS.seek 2.5ml @22:21:17.00 delay 1 second @22:21:18.01 PBV.dial :waste1 @22:21:18.40 PTV.dial :mixing @22:21:19.14 PSV.dial :mixloop @22:21:20.19 PS.seek :empty @22:21:46.53 PSV.dial PSR @22:21:47.56 PSR.select :flush @22:21:47.68 PS.seek 1ml @22:21:58.77 PSR.select :air @22:21:58.89 PS.seek 2.5ml @22:22:15.09 PSV.dial PTV @22:22:15.84 PTV.dial :puck @22:22:16.57 PBV.dial :waste1 @22:22:16.66 PS.seek 0ml @22:22:43.06 PSV.dial PSR @22:22:43.79 PSR.select :flush @22:22:43.91 PS.seek 0.5ml @22:22:49.90 PSR.select :air @22:22:50.47 PS.seek 2.5ml @22:23:11.74 PSV.dial PTV @22:23:12.49 PBV.dial PSV @22:23:13.08 PS.seek 0ml @22:23:39.65 PSV.dial PBV @22:23:40.11 PS.seek 2.5ml @22:24:06.49 PSV.dial :waste1 @22:24:07.23 PS.seek 0ml @22:24:33.62 PSV.dial PSR @22:24:34.20 PSR.select :air @22:24:34.22 PS.seek 2.5ml @22:25:00.77 PSV.dial PTV @22:25:01.52 PBV.dial PSV @22:25:01.61 PS.seek 0ml @22:25:28.18 PSV.dial PBV @22:25:28.64 PS.seek 2.5ml @22:25:54.93 PSV.dial :waste1 @22:25:55.67 PS.seek 0ml @22:26:22.02 PSV.dial PSR @22:26:22.52 PSR.select :flush @22:26:22.66 PS.seek 1ml @22:26:33.80 PSR.select :air @22:26:33.93 PS.seek 2.5ml @22:26:50.24 PSV.dial PTV @22:26:50.98 PTV.dial :puck @22:26:51.05 PBV.dial :waste1 @22:26:51.62 PS.seek 0ml @22:27:17.96 PSV.dial PSR @22:27:18.82 PSR.select :flush @22:27:18.94 PS.seek 0.5ml @22:27:24.93 PSR.select :air @22:27:25.04 PS.seek 2.5ml @22:27:46.33 PSV.dial PTV @22:27:47.07 PBV.dial PSV @22:27:47.64 PS.seek 0ml @22:28:14.02 PSV.dial PBV @22:28:14.48 PS.seek 2.5ml @22:28:40.80 PSV.dial :waste1 @22:28:41.55 PS.seek 0ml @22:29:07.89 PSV.dial PSR @22:29:08.36 PSR.select :air @22:29:08.39 PS.seek 2.5ml @22:29:34.74 PSV.dial PTV @22:29:35.91 PBV.dial PSV @22:29:36.00 PS.seek 0ml @22:30:02.36 PSV.dial PBV @22:30:02.95 PS.seek 2.5ml @22:30:29.24 PSV.dial :waste1 @22:30:30.00 PS.seek 0ml @22:30:56.43
Loading FlushPuck into Collection Clamp from Processing Clamp @22:30:56.62 Elbow.seek PC @22:30:59.13 Elbow.reconfigure :loose @22:30:59.23 Forearm.seek PC @22:31:00.34 Hand.close @22:31:01.04 PC.seek :open @22:31:05.54 Forearm.seek :retract @22:31:06.71 Elbow.reconfigure :tight @22:31:06.75 Elbow.seek CC @22:31:10.32 Elbow.reconfigure :loose @22:31:10.42 Forearm.reconfigure :tight @22:31:10.48 Forearm.seek CC @22:31:11.74 CC.seek :closed @22:31:15.72 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.00 @22:31:15.78 Hand.open @22:31:16.59 Forearm.reconfigure :loose @22:31:16.64 Forearm.seek :retract @22:31:17.66 Flushing the Collection Valve::Manifold @22:31:17.78 CSV.dial CSR @22:31:18.21 Pulling 5ml of CSR.flush @22:31:18.22 CSR.select :flush @22:31:18.34 CS.seek 5ml @22:31:32.40 delay 5 seconds @22:31:37.42 CSR.select :air @22:31:37.59 CS.reconfigure :fast @22:31:37.64 CS.seek 7.5ml @22:31:44.15 CSV.dial :waste @22:31:45.42 CS.reconfigure :default @22:31:45.47 CS.seek 0ml @22:32:05.46 Flushing lower manifold's waste1 port @22:32:05.58 CSV.dial :flush @22:32:06.03 CS.reconfigure :fast @22:32:06.08 CS.seek 5ml @22:32:18.27 delay 5 seconds @22:32:23.29 CSV.dial :air @22:32:23.81 CS.seek 7ml @22:32:29.27 CSV.dial CTV @22:32:29.30
CTV.dial CSV @22:32:30.68 CBV.dial :waste1 @22:32:31.45 CS.seek 0ml @22:32:48.06 Flushing lower manifold's waste2 port @22:32:48.18 CSV.dial :flush @22:32:48.92 CS.seek 5ml @22:33:01.12 delay 5 seconds @22:33:06.14 CSV.dial :air @22:33:06.66 CS.seek 6.999ml @22:33:12.15 CSV.dial CTV @22:33:12.18
CTV.dial CSV @22:33:12.62 CBV.dial :waste2 @22:33:13.67 CS.seek 0ml @22:33:30.37 Flushing lower manifold's waste2b port @22:33:30.49 CSV.dial :flush @22:33:31.23 CS.seek 5ml @22:33:43.43 delay 5 seconds @22:33:48.45 CSV.dial :air @22:33:48.97 CS.seek 7ml @22:33:54.46 CSV.dial CTV @22:33:54.50
CTV.dial CSV @22:33:54.93 CBV.dial :waste2b @22:33:55.97 CS.seek 0ml @22:34:12.52 Disinfecting external Intake with kill @22:34:12.65 CSV.dial CSR @22:34:13.09 Pulling 8ml of CSR.kill CSR.select :kill @22:34:13.21 CS.seek 8ml @22:34:32.40 delay 5 seconds @22:34:37.41 CSR.select :air @22:34:37.54 CS.seek 10ml @22:34:43.38 CSV.dial CBV @22:34:43.41
CBV.dial CSV @22:34:44.44 CTV.dial :intake @22:34:45.19 Intake.select :open @22:34:45.30 CS.seek 2ml @22:35:04.18 Intake.select :close @22:35:04.28 CTV.dial :air @22:35:05.06 CS.reconfigure :default @22:35:05.10 CS.seek 0ml @22:35:11.02 CSV.dial CSR @22:35:12.02 Pulling 5ml of CSR.flush @22:35:12.03 CSR.select :flush @22:35:12.15 CS.seek 5ml @22:35:25.86 delay 5 seconds @22:35:30.88 CSR.select :air @22:35:31.00 CS.seek 10ml @22:35:44.61 CSV.dial CBV @22:35:45.62 CS.seek 0ml @22:36:11.99 Garaging FlushPuck in Collection Clamp @22:36:12.06 Elbow.reconfigure :tight @22:36:12.11 Elbow.seek CC @22:36:12.22 Elbow.reconfigure :loose @22:36:12.32 Forearm.seek CC @22:36:13.31 Hand.close @22:36:14.03 CC.seek :open @22:36:18.10 Forearm.seek :retract @22:36:19.08 Elbow.reconfigure :tight @22:36:19.12 Elbow.seek :garage @22:36:21.99 Elbow.reconfigure :loose @22:36:22.09 Forearm.reconfigure :tight @22:36:22.13 Forearm.seek :garage @22:36:23.27 Hand.open @22:36:24.09 Forearm.reconfigure :loose @22:36:24.13 Forearm.seek :retract @22:36:25.12 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 20:15:52 @22:36:25.17 Asleep after completing DA_WCR_100825 assays @22:36:25.19 GoodNews.email "{1 => 22,\n 2 => 6,\n 3 => 16,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@22:35:02, 40% flow, 22.0C, 20% humidity, 14.7psia, 13.70V, 0.466A avg, 114.342Ah, 6.39W\nSampled 118.7ml",Subject: "Asleep after completing DA_WCR_100825 assays" @22:36:29.34 upsync + ESPwhich DA_WCR_101625.rb + fn=DA_WCR_101625.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_101625.rb + [ -f /home/esp/esp2/mission/DA_WCR_101625.rb ] + realpath /home/esp/esp2/mission/DA_WCR_101625.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_101625.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_101625.rb @12:39:01.48UTC16-Oct-25
ESP2 v3.42 I2C Gateway v3.20 for core bus @12:39:04.12 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @12:39:07.80 Polling Can every 10:00 @12:39:07.83 Cleanly exited at 12:36:49UTC16-Oct-25 @12:39:07.88 Loading /home/esp/esp2local/mission/DA_WCR_101625.rb mission @12:39:07.93 GoodNews.email "Starting DA_WCR_101625 w/pucks in tube 3\n{1 => 22,\n 2 => 6,\n 3 => 16,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@12:39:07, 41% flow, 19.1C, 20% humidity, 14.6psia, 13.74V, 0.340A avg, 151.349Ah, 4.68W",Subject: "DA_WCR_101625 begun" @12:39:07.97
Preparing to sleep until + 00:00:05 @12:39:08.00 Asleep til + 00:00:05 @12:39:08.02 Continuing at 12:39 @12:39:08.31 Gate.power :core,:OFF @12:39:08.33 Gate.power :raw,:OFF @12:39:08.34 Shutdown @12:39:10.56 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_101625.rb:14\n{1 => 22,\n 2 => 6,\n 3 => 16,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@12:39:08, 41% flow, 19.1C, 20% humidity, 14.6psia, 13.74V, 0.334A avg, 151.349Ah, 4.59W",Subject: "Asleep until 12:39" @12:39:11.96 upsync @12:39:12.92
Powering core bus @12:39:14.39 upsync @12:39:18.37
Gate.power :raw,:ON @12:39:19.38 CS.configure :default @12:39:19.42 PS.configure :default @12:39:19.47 SS.configure :default @12:39:19.52 CC.configure :default @12:39:19.57 PC.configure :default @12:39:19.64 Forearm.configure :loose @12:39:19.68 Elbow.configure :loose @12:39:19.75 SC.configure :loose @12:39:19.80 SE.configure :default @12:39:19.86 CH.configure :default @12:39:19.89 PH.configure :default @12:39:19.93 All dwarf controllers running firmware version 3.85 @12:39:20.04 Valve.reset @12:39:20.30 Intake.select :close @12:39:20.34 Exhaust.select :close @12:39:20.36 CTV.dial :air @12:39:20.43 PSV.dial :air @12:39:20.56 CSV.dial :waste1 @12:39:20.61
Forearm.home.seek :retract @12:39:21.03 SSV.dial :spit,avoiding: :exhaust @12:39:21.39 Hand.close @12:39:21.42
Elbow.reconfigure :loose @12:39:21.51 Elbow.jog 1500 @12:39:21.58 PS.home.seek 0ml @12:39:21.91 CS.reconfigure :fast @12:39:21.95 SS.home.seek 0ml @12:39:22.03 CS.home.seek 0ml @12:39:22.10
Elbow.reconfigure :loose @12:39:22.15 Elbow.home.seek :clear @12:39:25.32 SE.home.seek :down @12:39:26.01 SC.reconfigure :tight @12:39:26.06 SC.home.seek :initial @12:39:30.68 PS.stop @12:39:38.78
SC.configure :limp @12:39:38.84 Forearm.seek :retract @12:39:38.91 Elbow.seek :hammer @12:39:39.94 Forearm.reconfigure :tight @12:39:39.99 Forearm.seek retracted @12:39:40.55 PC.home.seek :open @12:39:40.63
CC.home.seek :open @12:39:43.77 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @12:39:43.80 Notice.email "At 12:39:43 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_101625.rb:14\n{1 => 22,\n 2 => 6,\n 3 => 16,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@12:39:43, 41% flow, 19.1C, 20% humidity, 14.6psia, 13.75V, 0.303A avg, 151.353Ah, 4.16W",Subject: "Started DA_WCR_101625 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @12:39:43.83
Starting initialPurge @12:39:43.84 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:39:43.91 Hand.open @12:39:44.75 Elbow.reconfigure :tight @12:39:44.80 Elbow.seek FlushPuck::Garage @12:39:46.64 Elbow.reconfigure :loose @12:39:46.75 Forearm.reconfigure :loose @12:39:46.80 Forearm.seek :garage @12:39:47.65 upsync @12:39:47.83
Hand.close @12:39:48.73 Forearm.seek :retract @12:39:49.89 Elbow.reconfigure :tight @12:39:49.94 Elbow.seek CC @12:39:52.78 Elbow.reconfigure :loose @12:39:52.88 Forearm.reconfigure :tight @12:39:52.93 Forearm.seek CC @12:39:54.19 CC.seek :closed @12:39:58.18 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.03 @12:39:58.26 Hand.open @12:39:59.05 Forearm.reconfigure :loose @12:39:59.10 Forearm.seek :retract @12:40:00.19 Flushing the Collection Valve::Manifold @12:40:00.31 CSV.dial CSR @12:40:01.70 Pulling 5ml of CSR.flush @12:40:01.71 CSR.select :flush @12:40:01.83 CS.reconfigure :default @12:40:01.87 CS.seek 5ml @12:40:15.92 delay 5 seconds @12:40:20.94 CSR.select :air @12:40:21.11 CS.reconfigure :fast @12:40:21.15 CS.seek 7.5ml @12:40:27.76 CSV.dial :waste @12:40:29.03 CS.reconfigure :default @12:40:29.08 CS.seek 0ml @12:40:49.14 Purging external Intake @12:40:49.26 CSV.dial :flush @12:40:49.72 CS.reconfigure :fast @12:40:49.77 CS.seek 10ml @12:41:13.73 delay 5 seconds @12:41:18.75 CSV.dial CBV @12:41:18.79
CBV.dial CSV @12:41:19.53 CTV.dial :intake @12:41:20.31 Intake.select :open @12:41:20.42 CS.seek 0ml @12:41:44.14 Intake.select :close @12:41:44.24 CTV.dial :air @12:41:45.03 Flushing upper manifold @12:41:45.15 CSV.dial :flush @12:41:45.89 CS.seek 5ml @12:41:58.26 delay 5 seconds @12:42:03.28 CSV.dial :air @12:42:03.79 CS.seek 7ml @12:42:09.33 CSV.dial CBV @12:42:09.35
CBV.dial CSV @12:42:10.50 CTV.dial :air @12:42:10.58 CS.seek 0ml @12:42:27.45 delay 7 seconds @12:42:34.48 CS.seek 3ml @12:42:42.29 CSV.dial :waste @12:42:42.74 CS.seek 0ml @12:42:50.59 CSV.dial :flush @12:42:51.03 CS.seek 3ml @12:42:58.85 delay 5 seconds @12:43:03.87 CSV.dial :air @12:43:04.37 CS.seek 5ml @12:43:09.86 CSV.dial :air @12:43:10.03 CS.seek 0ml @12:43:22.32 delay 7 seconds @12:43:29.35 CS.seek 3ml @12:43:37.42 CSV.dial :waste @12:43:38.14 CS.seek 0ml @12:43:45.95 Garaging FlushPuck in Collection Clamp @12:43:46.02 Elbow.reconfigure :tight @12:43:46.06 Elbow.seek CC @12:43:46.18 Elbow.reconfigure :loose @12:43:46.28 Forearm.seek CC @12:43:47.28 Hand.close @12:43:47.98 CC.seek :open @12:43:52.10 Forearm.seek :retract @12:43:53.14 Elbow.reconfigure :tight @12:43:53.19 Elbow.seek :garage @12:43:56.07 Elbow.reconfigure :loose @12:43:56.16 Forearm.reconfigure :tight @12:43:56.21 Forearm.seek :garage @12:43:57.25 Hand.open @12:43:58.04 Forearm.reconfigure :loose @12:43:58.08 Forearm.seek :retract @12:43:59.11 da sampling at most 1000ml, wcr at most 1000ml @12:43:59.14 DAfans.email "Started da in tube 3 at 12:43:59UTC16-Oct-25\n{1 => 22,\n 2 => 6,\n 3 => 16,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@12:39:43, 41% flow, 19.1C, 20% humidity, 14.6psia, 13.75V, 0.303A avg, 151.353Ah, 4.16W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @12:43:59.18
Loading sh1 puck from tube 3 into Collection Clamp @12:43:59.45 SC.reconfigure :tight @12:43:59.50 SC.seek 3 @12:44:02.98 upsync @12:44:03.21
SC.configure :limp @12:44:03.44 Elbow.reconfigure :tight @12:44:03.49 Elbow.seek 3 @12:44:05.42 Forearm.reconfigure :tight @12:44:05.46 Forearm.seek 3 + 800 counts @12:44:06.48 SE.reconfigure :pick @12:44:06.54 SE.seek :beamBreak @12:44:08.13 SE.configure :default @12:44:08.29 Elbow.seek 3 @12:44:08.60 Forearm.seek 3 + 300 counts @12:44:08.87 Forearm.reconfigure :loose @12:44:08.92 Hand.close @12:44:09.62 SE.seek :down @12:44:10.19
Forearm.seek :retract @12:44:11.02 SE.stop @12:44:11.23
Elbow.seek CC @12:44:12.83 Elbow.reconfigure :loose @12:44:12.93 Forearm.reconfigure :tight @12:44:12.98 Forearm.seek CC @12:44:14.88 CC.seek :closed @12:44:18.86 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.97 @12:44:18.87 Hand.open @12:44:19.66 Forearm.reconfigure :loose @12:44:19.71 Forearm.seek :retract @12:44:20.65 collectSample 1000ml w/bubble point of 24psi @12:44:20.66 Priming 7ml @12:44:20.68 Intake.select :close @12:44:20.69 Exhaust.select :close @12:44:20.70 SS.hold @12:44:20.90 Calibrating pressure and temperature sensors @12:44:20.93 CTV.dial :air @12:44:21.01 SSV.dial :spit,avoiding: :exhaust @12:44:21.09 SS.reconfigure :fast @12:44:21.13 SS.seek 5ml @12:44:24.32 SSV.dial CBV @12:44:24.34
CBV.dial SSV @12:44:25.71 delay 3.8 seconds @12:44:29.63 Sensor limits: Intake max 100.7psia; Outlet max 111.5psia @12:44:29.64 SSV.dial :spit @12:44:30.98 SS.seek 0ml @12:44:33.78 SSV.dial CBV @12:44:35.13 CTV.dial :intake @12:44:35.90 SSV.dial CBV @12:44:35.94
CBV.dial SSV @12:44:36.16 Allowing 40s for 7.0ml of remaining 7.0ml @12:44:36.17 Intake.select :open @12:44:38.33 Intake @14.3psia, vacuum from 8.8 to 11.8psi @12:44:38.35 SS.reconfigure :inhaling 2.17ml/s @12:44:38.40 SS.seek 7ml @12:44:44.00 Stroke completed -- waiting for pressure to equalize ... @12:44:44.01 SS.reconfigure :exhale @12:44:44.05 SS.hold @12:44:44.66 Intake.select :close @12:44:44.81 SSV.dial :exhaust @12:44:45.65 Exhaust.select :open @12:44:45.76 SS.seek 0ml @12:44:50.03 Exhaust.select :close @12:44:50.14 Sampling 1000.0ml @12:44:50.16 Intake.select :close @12:44:50.17 Exhaust.select :close @12:44:50.18 SS.reconfigure :default @12:44:50.22 SS.hold @12:44:50.43 CTV.dial :intake @12:44:50.61 SSV.dial CBV @12:44:50.63
CBV.dial SSV @12:44:51.42 Allowing 130s for 25.0ml of remaining 1000.0ml @12:44:51.43 Intake.select :open @12:44:53.60 Intake @14.3psia, vacuum from 8.8 to 11.8psi @12:44:53.62 SS.reconfigure :inhaling 2.17ml/s @12:44:53.66 SS.seek 25ml @12:45:07.62 Stroke completed -- waiting for pressure to equalize ... @12:45:07.63 SS.reconfigure :exhale @12:45:07.68 SS.hold @12:45:14.16 Intake.select :close @12:45:14.31 SSV.dial :exhaust @12:45:15.08 Exhaust.select :open @12:45:15.19 SS.seek 0ml @12:45:24.47 Exhaust.select :close @12:45:24.58 SSV.dial CBV @12:45:24.60
CBV.dial SSV @12:45:25.41 Allowing 130s for 25.0ml of remaining 975.0ml @12:45:25.42 Intake.select :open @12:45:27.58 Intake @13.9psia, vacuum from 8.4 to 11.4psi @12:45:27.60 SS.reconfigure :inhaling 2.17ml/s @12:45:27.65 SS.seek 25ml @12:45:41.75 Stroke completed -- waiting for pressure to equalize ... @12:45:41.76 SS.reconfigure :exhale @12:45:41.80 SS.hold @12:45:48.69 Intake.select :close @12:45:48.84 SSV.dial :exhaust @12:45:49.63 Exhaust.select :open @12:45:49.75 SS.seek 0ml @12:45:59.08 Exhaust.select :close @12:45:59.19 SSV.dial CBV @12:45:59.21
CBV.dial SSV @12:46:00.01 Allowing 130s for 25.0ml of remaining 950.0ml @12:46:00.03 Intake.select :open @12:46:02.19 Intake @13.9psia, vacuum from 8.4 to 11.4psi @12:46:02.21 SS.reconfigure :inhaling 2.17ml/s @12:46:02.25 SS.seek 25ml @12:46:17.33 Stroke completed -- waiting for pressure to equalize ... @12:46:17.34 SS.reconfigure :exhale @12:46:17.38 SS.hold @12:46:24.78 Intake.select :close @12:46:24.93 SSV.dial :exhaust @12:46:25.70 Exhaust.select :open @12:46:26.23 SS.seek 0ml @12:46:35.48 Exhaust.select :close @12:46:35.59 SSV.dial CBV @12:46:35.61
CBV.dial SSV @12:46:36.44 Allowing 130s for 25.0ml of remaining 925.0ml @12:46:36.45 Intake.select :open @12:46:38.61 Intake @13.9psia, vacuum from 8.4 to 11.4psi @12:46:38.63 SS.reconfigure :inhaling 2.17ml/s @12:46:38.68 SS.seek 25ml @12:46:52.82 Stroke completed -- waiting for pressure to equalize ... @12:46:52.83 SS.reconfigure :exhale @12:46:52.88 SS.hold @12:47:00.28 Intake.select :close @12:47:00.43 SSV.dial :exhaust @12:47:01.22 Exhaust.select :open @12:47:01.33 SS.seek 0ml @12:47:10.20 Exhaust.select :close @12:47:10.31 SSV.dial CBV @12:47:10.33
CBV.dial SSV @12:47:11.12 Allowing 130s for 25.0ml of remaining 900.0ml @12:47:11.13 Intake.select :open @12:47:13.29 Intake @13.9psia, vacuum from 8.4 to 11.4psi @12:47:13.31 SS.reconfigure :inhaling 2.17ml/s @12:47:13.36 SS.seek 25ml @12:47:27.71 Stroke completed -- waiting for pressure to equalize ... @12:47:27.72 SS.reconfigure :exhale @12:47:27.77 SS.hold @12:47:35.34 Intake.select :close @12:47:35.49 SSV.dial :exhaust @12:47:36.28 Exhaust.select :open @12:47:36.40 SS.seek 0ml @12:47:45.15 Exhaust.select :close @12:47:45.26 SSV.dial CBV @12:47:45.29
CBV.dial SSV @12:47:46.09 Allowing 130s for 25.0ml of remaining 875.0ml @12:47:46.10 Intake.select :open @12:47:48.26 Intake @13.8psia, vacuum from 8.3 to 11.3psi @12:47:48.28 SS.reconfigure :inhaling 2.17ml/s @12:47:48.33 SS.seek 25ml @12:48:02.37 Stroke completed -- waiting for pressure to equalize ... @12:48:02.38 SS.reconfigure :exhale @12:48:02.43 SS.hold @12:48:10.95 Intake.select :close @12:48:11.10 SSV.dial :exhaust @12:48:11.89 Exhaust.select :open @12:48:12.00 SS.seek 0ml @12:48:21.79 Exhaust.select :close @12:48:21.90 SSV.dial CBV @12:48:21.92
CBV.dial SSV @12:48:22.72 Allowing 130s for 25.0ml of remaining 850.0ml @12:48:22.73 Intake.select :open @12:48:24.89 Intake @14.0psia, vacuum from 8.5 to 11.5psi @12:48:24.91 SS.reconfigure :inhaling 2.17ml/s @12:48:24.96 SS.seek 25ml @12:48:38.85 Stroke completed -- waiting for pressure to equalize ... @12:48:38.86 SS.reconfigure :exhale @12:48:38.91 SS.hold @12:48:48.66 Intake.select :close @12:48:48.81 SSV.dial :exhaust @12:48:49.60 Exhaust.select :open @12:48:49.72 SS.seek 0ml @12:48:59.12 Exhaust.select :close @12:48:59.23 SSV.dial CBV @12:48:59.27
CBV.dial SSV @12:49:00.03 Allowing 130s for 25.0ml of remaining 825.0ml @12:49:00.04 Intake.select :open @12:49:02.20 Intake @13.8psia, vacuum from 8.3 to 11.3psi @12:49:02.22 SS.reconfigure :inhaling 2.17ml/s @12:49:02.27 SS.seek 25ml @12:49:16.35 Stroke completed -- waiting for pressure to equalize ... @12:49:16.36 SS.reconfigure :exhale @12:49:16.41 SS.hold @12:49:26.34 Intake.select :close @12:49:26.50 SSV.dial :exhaust @12:49:27.28 Exhaust.select :open @12:49:27.39 SS.seek 0ml @12:49:36.53 Exhaust.select :close @12:49:36.63 SSV.dial CBV @12:49:36.66
CBV.dial SSV @12:49:37.45 Allowing 130s for 25.0ml of remaining 800.0ml @12:49:37.46 Intake.select :open @12:49:39.62 Intake @14.1psia, vacuum from 8.6 to 11.6psi @12:49:39.64 SS.reconfigure :inhaling 2.17ml/s @12:49:39.69 SS.seek 25ml @12:49:53.77 Stroke completed -- waiting for pressure to equalize ... @12:49:53.78 SS.reconfigure :exhale @12:49:53.83 SS.hold @12:50:03.62 Intake.select :close @12:50:03.78 SSV.dial :exhaust @12:50:04.56 Exhaust.select :open @12:50:04.67 SS.seek 0ml @12:50:13.93 Exhaust.select :close @12:50:14.04 SSV.dial CBV @12:50:14.07
CBV.dial SSV @12:50:14.85 Allowing 130s for 25.0ml of remaining 775.0ml @12:50:14.86 Intake.select :open @12:50:17.03 Intake @13.9psia, vacuum from 8.4 to 11.4psi @12:50:17.05 SS.reconfigure :inhaling 2.17ml/s @12:50:17.10 SS.seek 25ml @12:50:31.10 Stroke completed -- waiting for pressure to equalize ... @12:50:31.11 SS.reconfigure :exhale @12:50:31.16 SS.hold @12:50:42.27 Intake.select :close @12:50:42.42 SSV.dial :exhaust @12:50:43.18 Exhaust.select :open @12:50:43.29 SS.seek 0ml @12:50:52.35 Exhaust.select :close @12:50:52.46 SSV.dial CBV @12:50:52.49
CBV.dial SSV @12:50:53.27 Allowing 130s for 25.0ml of remaining 750.0ml @12:50:53.29 Intake.select :open @12:50:55.44 Intake @14.0psia, vacuum from 8.5 to 11.5psi @12:50:55.46 SS.reconfigure :inhaling 2.17ml/s @12:50:55.51 SS.seek 25ml @12:51:10.16 Stroke completed -- waiting for pressure to equalize ... @12:51:10.17 SS.reconfigure :exhale @12:51:10.22 SS.hold @12:51:22.88 Intake.select :close @12:51:23.03 SSV.dial :exhaust @12:51:23.81 Exhaust.select :open @12:51:23.92 SS.seek 0ml @12:51:33.26 Exhaust.select :close @12:51:33.37 SSV.dial CBV @12:51:33.39
CBV.dial SSV @12:51:34.18 Allowing 130s for 25.0ml of remaining 725.0ml @12:51:34.19 Intake.select :open @12:51:36.35 Intake @14.0psia, vacuum from 8.5 to 11.5psi @12:51:36.37 SS.reconfigure :inhaling 2.17ml/s @12:51:36.42 SS.seek 25ml @12:51:50.83 Stroke completed -- waiting for pressure to equalize ... @12:51:50.84 SS.reconfigure :exhale @12:51:50.89 SS.hold @12:52:03.55 Intake.select :close @12:52:03.70 SSV.dial :exhaust @12:52:04.46 Exhaust.select :open @12:52:04.57 SS.seek 0ml @12:52:13.80 Exhaust.select :close @12:52:13.91 SSV.dial CBV @12:52:13.93
CBV.dial SSV @12:52:14.73 Allowing 130s for 25.0ml of remaining 700.0ml @12:52:14.74 Intake.select :open @12:52:16.90 Intake @14.1psia, vacuum from 8.6 to 11.6psi @12:52:16.92 SS.reconfigure :inhaling 2.17ml/s @12:52:16.97 SS.seek 25ml @12:52:31.21 Stroke completed -- waiting for pressure to equalize ... @12:52:31.22 SS.reconfigure :exhale @12:52:31.26 SS.hold @12:52:47.46 Intake.select :close @12:52:47.61 SSV.dial :exhaust @12:52:48.38 Exhaust.select :open @12:52:48.49 SS.seek 0ml @12:52:57.65 Exhaust.select :close @12:52:57.76 SSV.dial CBV @12:52:57.78
CBV.dial SSV @12:52:58.57 Allowing 130s for 25.0ml of remaining 675.0ml @12:52:58.58 Intake.select :open @12:53:00.74 Intake @13.9psia, vacuum from 8.4 to 11.4psi @12:53:00.76 SS.reconfigure :inhaling 2.17ml/s @12:53:00.81 SS.seek 25ml @12:53:15.11 Stroke completed -- waiting for pressure to equalize ... @12:53:15.12 SS.reconfigure :exhale @12:53:15.17 SS.hold @12:53:31.88 Intake.select :close @12:53:32.04 SSV.dial :exhaust @12:53:32.82 Exhaust.select :open @12:53:32.94 SS.seek 0ml @12:53:42.27 Exhaust.select :close @12:53:42.38 SSV.dial CBV @12:53:42.42
CBV.dial SSV @12:53:43.18 Allowing 130s for 25.0ml of remaining 650.0ml @12:53:43.19 Intake.select :open @12:53:45.35 Intake @14.4psia, vacuum from 8.9 to 11.9psi @12:53:45.37 SS.reconfigure :inhaling 2.17ml/s @12:53:45.42 SS.seek 25ml @12:53:59.85 Stroke completed -- waiting for pressure to equalize ... @12:53:59.86 SS.reconfigure :exhale @12:53:59.90 SS.hold @12:54:18.23 Intake.select :close @12:54:18.38 SSV.dial :exhaust @12:54:19.16 Exhaust.select :open @12:54:19.27 SS.seek 0ml @12:54:28.19 Exhaust.select :close @12:54:28.30 SSV.dial CBV @12:54:28.32
CBV.dial SSV @12:54:29.55 Allowing 130s for 25.0ml of remaining 625.0ml @12:54:29.56 Intake.select :open @12:54:31.72 Intake @14.2psia, vacuum from 8.7 to 11.7psi @12:54:31.74 SS.reconfigure :inhaling 2.17ml/s @12:54:31.79 SS.seek 25ml @12:54:43.12 Vacuum too high -- waiting up to 40 seconds @12:54:43.13 SS.reconfigure :exhale @12:54:43.19 SS.hold @12:54:46.50 Intake @11.4psia, vacuum from 5.9 to 8.9psi @12:54:46.51 SS.reconfigure :inhaling 1.95ml/s @12:54:46.56 SS.seek 25ml @12:54:51.17 Stroke completed -- waiting for pressure to equalize ... @12:54:51.18 SS.reconfigure :exhale @12:54:51.23 SS.hold @12:55:07.51 Intake.select :close @12:55:07.67 SSV.dial :exhaust @12:55:08.44 Exhaust.select :open @12:55:08.56 SS.seek 0ml @12:55:17.42 Exhaust.select :close @12:55:17.53 SSV.dial CBV @12:55:17.56
CBV.dial SSV @12:55:18.35 Allowing 130s for 25.0ml of remaining 600.0ml @12:55:18.36 Intake.select :open @12:55:20.52 Intake @14.3psia, vacuum from 8.8 to 11.8psi @12:55:20.54 SS.reconfigure :inhaling 1.95ml/s @12:55:20.59 SS.seek 25ml @12:55:31.18 Vacuum too high -- waiting up to 40 seconds @12:55:31.20 SS.reconfigure :exhale @12:55:31.25 SS.hold @12:55:32.15 Intake @11.6psia, vacuum from 6.1 to 9.1psi @12:55:32.17 SS.reconfigure :inhaling 1.77ml/s @12:55:32.21 SS.seek 25ml @12:55:35.28 Vacuum too high -- waiting up to 40 seconds @12:55:35.29 SS.reconfigure :exhale @12:55:35.34 SS.hold @12:55:50.41 Intake @12.7psia, vacuum from 7.2 to 10.2psi @12:55:50.42 SS.reconfigure :inhaling 1.59ml/s @12:55:50.47 SS.seek 25ml @12:55:55.05 Stroke completed -- waiting for pressure to equalize ... @12:55:55.06 SS.reconfigure :exhale @12:55:55.11 SS.hold @12:56:01.65 Intake.select :close @12:56:01.80 SSV.dial :exhaust @12:56:02.58 Exhaust.select :open @12:56:02.70 SS.seek 0ml @12:56:12.08 Exhaust.select :close @12:56:12.19 SSV.dial CBV @12:56:12.21
CBV.dial SSV @12:56:13.00 Allowing 130s for 25.0ml of remaining 575.0ml @12:56:13.01 Intake.select :open @12:56:15.18 Intake @14.2psia, vacuum from 8.7 to 11.7psi @12:56:15.19 SS.reconfigure :inhaling 1.59ml/s @12:56:15.24 SS.seek 25ml @12:56:23.52 Vacuum too high -- waiting up to 40 seconds @12:56:23.54 SS.reconfigure :exhale @12:56:23.59 SS.hold @12:56:28.42 Intake @12.2psia, vacuum from 6.7 to 9.7psi @12:56:28.44 SS.reconfigure :inhaling 1.43ml/s @12:56:28.48 SS.seek 25ml @12:56:34.74 Vacuum too high -- waiting up to 40 seconds @12:56:34.76 SS.reconfigure :exhale @12:56:34.81 SS.hold @12:56:43.52 Intake @13.1psia, vacuum from 7.6 to 10.6psi @12:56:43.54 SS.reconfigure :inhaling 1.29ml/s @12:56:43.59 SS.seek 25ml @12:56:49.84 Stroke completed -- waiting for pressure to equalize ... @12:56:49.85 SS.reconfigure :exhale @12:56:49.90 SS.hold @12:57:00.12 Intake.select :close @12:57:00.27 SSV.dial :exhaust @12:57:01.03 Exhaust.select :open @12:57:01.14 SS.seek 0ml @12:57:10.44 Exhaust.select :close @12:57:10.55 SSV.dial CBV @12:57:10.57
CBV.dial SSV @12:57:11.38 Allowing 130s for 25.0ml of remaining 550.0ml @12:57:11.39 Intake.select :open @12:57:13.55 Intake @14.4psia, vacuum from 8.9 to 11.9psi @12:57:13.57 SS.reconfigure :inhaling 1.29ml/s @12:57:13.62 SS.seek 25ml @12:57:25.02 Vacuum too high -- waiting up to 40 seconds @12:57:25.04 SS.reconfigure :exhale @12:57:25.09 SS.hold @12:57:30.09 Intake @12.2psia, vacuum from 6.7 to 9.7psi @12:57:30.10 SS.reconfigure :inhaling 1.16ml/s @12:57:30.15 SS.seek 25ml @12:57:35.02 Vacuum too high -- waiting up to 40 seconds @12:57:35.04 SS.reconfigure :exhale @12:57:35.10 SS.hold @12:57:42.76 Intake @12.8psia, vacuum from 7.3 to 10.3psi @12:57:42.77 SS.reconfigure :inhaling 1.04ml/s @12:57:42.82 SS.seek 25ml @12:57:50.45 Stroke completed -- waiting for pressure to equalize ... @12:57:50.46 SS.reconfigure :exhale @12:57:50.51 SS.hold @12:58:01.62 Intake.select :close @12:58:01.77 SSV.dial :exhaust @12:58:02.53 Exhaust.select :open @12:58:02.64 SS.seek 0ml @12:58:12.00 Exhaust.select :close @12:58:12.11 SSV.dial CBV @12:58:12.13
CBV.dial SSV @12:58:12.94 Allowing 130s for 25.0ml of remaining 525.0ml @12:58:12.95 Intake.select :open @12:58:15.54 Intake @14.4psia, vacuum from 8.9 to 11.9psi @12:58:15.56 SS.reconfigure :inhaling 1.04ml/s @12:58:15.61 SS.seek 25ml @12:58:30.65 Vacuum too high -- waiting up to 40 seconds @12:58:30.66 SS.reconfigure :exhale @12:58:30.71 SS.hold @12:58:39.36 Intake @13.3psia, vacuum from 7.8 to 10.8psi @12:58:39.38 SS.reconfigure :inhaling 0.94ml/s @12:58:39.42 SS.seek 25ml @12:58:49.08 Vacuum too high -- waiting up to 40 seconds @12:58:49.10 SS.reconfigure :exhale @12:58:49.15 SS.hold @12:58:58.28 Intake @13.6psia, vacuum from 8.1 to 11.1psi @12:58:58.30 SS.reconfigure :inhaling 0.85ml/s @12:58:58.35 SS.seek 25ml @12:59:01.65 Stroke completed -- waiting for pressure to equalize ... @12:59:01.66 SS.reconfigure :exhale @12:59:01.71 SS.hold @12:59:09.16 Intake.select :close @12:59:09.31 SSV.dial :exhaust @12:59:10.09 Exhaust.select :open @12:59:10.20 SS.seek 0ml @12:59:19.56 Exhaust.select :close @12:59:19.67 SSV.dial CBV @12:59:19.69
CBV.dial SSV @12:59:20.48 Allowing 130s for 25.0ml of remaining 500.0ml @12:59:20.49 Intake.select :open @12:59:22.65 Intake @14.4psia, vacuum from 8.9 to 11.9psi @12:59:22.67 SS.reconfigure :inhaling 0.85ml/s @12:59:22.72 SS.seek 25ml @12:59:38.36 Vacuum too high -- waiting up to 40 seconds @12:59:38.38 SS.reconfigure :exhale @12:59:38.43 SS.hold @12:59:45.44 Intake @13.3psia, vacuum from 7.8 to 10.8psi @12:59:45.46 SS.reconfigure :inhaling 0.77ml/s @12:59:45.51 SS.seek 25ml @12:59:55.30 Vacuum too high -- waiting up to 40 seconds @12:59:55.31 SS.reconfigure :exhale @12:59:55.37 SS.hold @13:00:02.96 Intake @13.7psia, vacuum from 8.2 to 11.2psi @13:00:02.97 SS.reconfigure :inhaling 0.70ml/s @13:00:03.02 SS.seek 25ml @13:00:12.11 Stroke completed -- waiting for pressure to equalize ... @13:00:12.12 SS.reconfigure :exhale @13:00:12.16 SS.hold @13:00:22.26 Intake.select :close @13:00:22.41 SSV.dial :exhaust @13:00:23.19 Exhaust.select :open @13:00:23.30 SS.seek 0ml @13:00:32.69 Exhaust.select :close @13:00:32.79 SSV.dial CBV @13:00:32.82
CBV.dial SSV @13:00:33.61 Allowing 130s for 25.0ml of remaining 475.0ml @13:00:33.62 Intake.select :open @13:00:35.78 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:00:35.80 SS.reconfigure :inhaling 0.70ml/s @13:00:35.85 SS.seek 25ml @13:00:59.21 Vacuum too high -- waiting up to 40 seconds @13:00:59.22 SS.reconfigure :exhale @13:00:59.27 SS.hold @13:01:07.94 Intake @13.6psia, vacuum from 8.1 to 11.1psi @13:01:07.95 SS.reconfigure :inhaling 0.62ml/s @13:01:08.00 SS.seek 25ml @13:01:24.01 Stroke completed -- waiting for pressure to equalize ... @13:01:24.02 SS.reconfigure :exhale @13:01:24.07 SS.hold @13:01:38.42 Intake.select :close @13:01:38.57 SSV.dial :exhaust @13:01:39.34 Exhaust.select :open @13:01:39.45 SS.seek 0ml @13:01:48.85 Exhaust.select :close @13:01:48.96 SSV.dial CBV @13:01:48.99
CBV.dial SSV @13:01:49.78 Allowing 130s for 25.0ml of remaining 450.0ml @13:01:49.79 Intake.select :open @13:01:51.95 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:01:51.97 SS.reconfigure :inhaling 0.62ml/s @13:01:52.02 SS.seek 25ml @13:02:19.29 Vacuum too high -- waiting up to 40 seconds @13:02:19.30 SS.reconfigure :exhale @13:02:19.35 SS.hold @13:02:25.93 Intake @13.0psia, vacuum from 7.5 to 10.5psi @13:02:25.95 SS.reconfigure :inhaling 0.56ml/s @13:02:25.99 SS.seek 25ml @13:02:42.24 Stroke completed -- waiting for pressure to equalize ... @13:02:42.25 SS.reconfigure :exhale @13:02:42.30 SS.hold @13:02:58.59 Intake.select :close @13:02:58.75 SSV.dial :exhaust @13:02:59.53 Exhaust.select :open @13:02:59.64 SS.seek 0ml @13:03:09.15 Exhaust.select :close @13:03:09.26 SSV.dial CBV @13:03:09.28
CBV.dial SSV @13:03:10.09 Allowing 130s for 25.0ml of remaining 425.0ml @13:03:10.10 Intake.select :open @13:03:12.26 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:03:12.28 SS.reconfigure :inhaling 0.56ml/s @13:03:12.33 SS.seek 25ml @13:03:38.39 Vacuum too high -- waiting up to 40 seconds @13:03:38.40 SS.reconfigure :exhale @13:03:38.46 SS.hold @13:03:48.36 Intake @13.6psia, vacuum from 8.1 to 11.1psi @13:03:48.38 SS.reconfigure :inhaling 0.51ml/s @13:03:48.43 SS.seek 25ml @13:04:08.08 Vacuum too high -- waiting up to 40 seconds @13:04:08.09 SS.reconfigure :exhale @13:04:08.14 SS.hold @13:04:22.91 Intake @13.7psia, vacuum from 8.2 to 11.2psi @13:04:22.93 SS.reconfigure :inhaling 0.46ml/s @13:04:22.98 SS.seek 25ml @13:04:26.41 Stroke completed -- waiting for pressure to equalize ... @13:04:26.42 SS.reconfigure :exhale @13:04:26.47 SS.hold @13:04:37.69 Intake.select :close @13:04:37.84 SSV.dial :exhaust @13:04:38.62 Exhaust.select :open @13:04:38.73 SS.seek 0ml @13:04:48.18 Exhaust.select :close @13:04:48.29 SSV.dial CBV @13:04:48.33
CBV.dial SSV @13:04:49.09 Allowing 130s for 25.0ml of remaining 400.0ml @13:04:49.10 Intake.select :open @13:04:51.26 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:04:51.28 SS.reconfigure :inhaling 0.46ml/s @13:04:51.33 SS.seek 25ml @13:05:16.29 Vacuum too high -- waiting up to 40 seconds @13:05:16.31 SS.reconfigure :exhale @13:05:16.36 SS.hold @13:05:31.26 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:05:31.28 SS.reconfigure :inhaling 0.42ml/s @13:05:31.33 SS.seek 25ml @13:05:49.67 Vacuum too high -- waiting up to 40 seconds @13:05:49.68 SS.reconfigure :exhale @13:05:49.74 SS.hold @13:06:06.14 Intake @14.1psia, vacuum from 8.6 to 11.6psi @13:06:06.16 SS.reconfigure :inhaling 0.38ml/s @13:06:06.21 SS.seek 25ml @13:06:24.10 Stroke completed -- waiting for pressure to equalize ... @13:06:24.11 SS.reconfigure :exhale @13:06:24.15 SS.hold @13:06:54.81 Intake.select :close @13:06:54.96 SSV.dial :exhaust @13:06:55.72 Exhaust.select :open @13:06:55.84 SS.seek 0ml @13:07:05.40 Exhaust.select :close @13:07:05.51 SSV.dial CBV @13:07:05.53
CBV.dial SSV @13:07:06.32 Allowing 130s for 25.0ml of remaining 375.0ml @13:07:06.33 Intake.select :open @13:07:08.49 Intake @14.9psia, vacuum from 9.4 to 12.4psi @13:07:08.51 SS.reconfigure :inhaling 0.38ml/s @13:07:08.56 SS.seek 25ml @13:07:43.26 Vacuum too high -- waiting up to 40 seconds @13:07:43.27 SS.reconfigure :exhale @13:07:43.32 SS.hold @13:07:58.22 Intake @14.1psia, vacuum from 8.6 to 11.6psi @13:07:58.24 SS.reconfigure :inhaling 0.33ml/s @13:07:58.29 SS.seek 25ml @13:08:24.70 Vacuum too high -- waiting up to 40 seconds @13:08:24.71 SS.reconfigure :exhale @13:08:24.76 SS.hold @13:09:04.95 SS.reconfigure :default @13:09:05.00 SS.hold @13:09:05.07 Intake.select :close @13:09:05.22 ShallowSampler::Clogged in MAIN -- Intake @14.8psia, & Outlet @4.8psia -- no progress after 645.9ml @13:09:05.24 SSV.dial :exhaust @13:09:06.00 Exhaust.select :open @13:09:06.11 SS.reconfigure :exhale @13:09:06.16 SS.seek 0ml @13:09:14.74 Exhaust.select :close @13:09:14.85 Sampled 645.9ml @13:09:14.86 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:09:14.87 Intake.select :close @13:09:14.93 CSV.dial :waste @13:09:15.08 SSV.dial CBV,avoiding: :exhaust @13:09:15.12 CS.seek 0ml @13:09:15.19
CBV.dial SSV @13:09:15.29 CSV.dial :air @13:09:16.02 CS.seek 10ml @13:09:17.07
CTV.dial :air @13:09:17.83 delay 15 seconds @13:09:32.85 SSV.dial :spit,avoiding: :exhaust @13:09:34.19 CTV.dial CSV,avoiding: :intake @13:09:34.23
SS.seek 0ml @13:09:34.35 SSV.dial CBV,avoiding: :exhaust @13:09:40.24 SSV.dial between CBV and plug @13:09:40.72 CBV.dial between SSV and waste2 @13:09:41.57 CTV.dial CSV,avoiding: :air @13:09:41.59
CSV.dial CTV @13:09:42.04 CS.reconfigure :pressurize @13:09:42.09 CS.seek 0ml @13:10:05.96 BubblePoint: Intake @24.0psia, Outlet @0.1psia @13:10:05.97 CBV.dial SSV @13:10:06.32 SS.reconfigure :default @13:10:06.37 SS.seek 10ml @13:10:11.96 delay 15 seconds @13:10:27.09 Evacuated: Intake @23.7psia, Outlet @23.3psia @13:10:27.10 SSV.dial :spit,avoiding: :exhaust @13:10:28.16 SS.reconfigure :exhale @13:10:28.21 SS.seek 0ml @13:10:33.24 SS.reconfigure :fast @13:10:33.29 SS.seek 5ml @13:10:36.26 SSV.dial CBV,avoiding: :exhaust @13:10:36.28
CBV.dial SSV @13:10:37.68 CTV.dial :air,avoiding: :intake @13:10:39.05 CSV.dial CTV @13:10:39.09
SS.seek 10ml @13:10:40.29 CSV.dial :air @13:10:40.85 CS.reconfigure :fast @13:10:40.90 CS.seek 0ml @13:10:41.96
SSV.dial :spit,avoiding: :exhaust @13:10:43.30 SS.reconfigure :exhale @13:10:43.35 SS.seek 0ml @13:10:43.67 CS.stop @13:10:48.65
SS.reconfigure :fast @13:10:48.70 SS.seek 5ml @13:10:51.30 SSV.dial CBV,avoiding: :exhaust @13:10:51.34
CBV.dial SSV @13:10:53.70 SSV.dial :spit,avoiding: :exhaust @13:10:55.05 SS.reconfigure :default @13:10:55.10 SS.seek 0ml @13:10:58.30 SS.stop @13:10:58.34 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @13:10:58.41 CSV.dial :waste @13:10:58.53 SSV.dial CBV,avoiding: :exhaust @13:10:58.56
CBV.dial SSV @13:10:59.18 CS.seek 0ml @13:10:59.25 CSV.dial :air @13:10:59.94
CTV.dial :air @13:11:00.02 CS.seek 10ml @13:11:00.13
delay 15 seconds @13:11:15.15 SSV.dial :spit,avoiding: :exhaust @13:11:16.47 CTV.dial CSV,avoiding: :intake @13:11:16.87
SS.reconfigure :exhale @13:11:17.03 SS.seek 0ml @13:11:17.09 SSV.dial CBV,avoiding: :exhaust @13:11:24.08 SSV.dial between CBV and plug @13:11:24.58 CBV.dial between SSV and waste2 @13:11:24.97 CSV.dial between air and CTV @13:11:25.28 CS.seek 8.022ml @13:11:30.68 CTV.dial CSV,avoiding: :air @13:11:30.72
CSV.dial CTV @13:11:31.05 CS.reconfigure :pressurize @13:11:31.10 CS.seek 0ml @13:11:49.77 BubblePoint: Intake @24.0psia, Outlet @-0.5psia @13:11:49.78 CBV.dial SSV @13:11:50.13 SS.reconfigure :default @13:11:50.18 SS.seek 10ml @13:11:55.67 delay 15 seconds @13:12:10.80 Evacuated: Intake @22.6psia, Outlet @15.6psia @13:12:10.81 SSV.dial :spit,avoiding: :exhaust @13:12:11.86 SS.reconfigure :exhale @13:12:11.91 SS.seek 0ml @13:12:16.99 SS.reconfigure :fast @13:12:17.04 SS.seek 5ml @13:12:20.11 SSV.dial CBV,avoiding: :exhaust @13:12:20.14
CBV.dial SSV @13:12:21.51 CTV.dial :air,avoiding: :intake @13:12:22.88 CSV.dial CTV @13:12:22.91
SS.seek 10ml @13:12:24.04 CSV.dial :air @13:12:24.49 CS.reconfigure :fast @13:12:24.54 CS.seek 0ml @13:12:25.81
SSV.dial :spit,avoiding: :exhaust @13:12:27.14 SS.reconfigure :exhale @13:12:27.19 SS.seek 0ml @13:12:27.58 CS.stop @13:12:32.15
SS.reconfigure :fast @13:12:32.21 SS.seek 5ml @13:12:34.96 SSV.dial CBV,avoiding: :exhaust @13:12:34.98
CBV.dial SSV @13:12:37.35 SSV.dial :spit,avoiding: :exhaust @13:12:38.69 SS.reconfigure :default @13:12:38.74 SS.seek 0ml @13:12:42.17 SS.stop @13:12:42.21 Lysing: Adding 2.0ml AqMeOH @13:12:42.22 CBV.dial between CSV and waste1,avoiding: :DBV @13:12:44.16 CTV.dial CSV @13:12:45.50 CSV.dial CSR @13:12:46.29 Pulling 1ml of CSR.AqMeOH @13:12:46.30 CSR.select :AqMeOH @13:12:46.42 CS.reconfigure :default @13:12:46.46 CS.seek 1ml @13:12:49.86 delay 5 seconds @13:12:54.88 CSR.select :air @13:12:55.05 CS.seek 6ml @13:13:08.67 delay 2 seconds @13:13:10.69 CSV.dial :waste2 @13:13:11.13 CS.reconfigure :fast @13:13:11.18 CS.seek 0ml @13:13:25.58 CSV.dial CSR @13:13:26.03 Pulling 2ml of CSR.AqMeOH @13:13:26.04 CSR.select :AqMeOH @13:13:26.16 CS.reconfigure :default @13:13:26.20 CS.seek 2ml @13:13:32.08 delay 5 seconds @13:13:37.10 CSR.select :air @13:13:37.27 CS.seek 4.999ml @13:13:45.77 delay 2 seconds @13:13:47.78 CSV.dial CTV @13:13:48.23 CS.seek 1ml @13:13:59.37 Before lysing Collection Puck Heater at 16C/15.6psia @13:13:59.39 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @13:13:59.41 CH.seek 70C,8:00 @13:16:21.46 CH.hold 10:00,70C @13:16:21.66 Begun lysing Collection Puck Heater at 68C/22.2psia @13:26:21.67 Ended lysing Collection Puck Heater at 70C/19.8psia @13:26:21.68 CS.seek 0ml @13:26:25.07 CTV.dial between spare and air,avoiding: :intake @13:26:26.13 CSV.dial :air @13:26:27.02 CS.seek 5ml @13:26:40.66 CSV.dial CTV @13:26:41.12 delay 2 seconds @13:26:43.13 CSV.dial :air @13:26:43.58 delay 2 seconds @13:26:45.60 CSV.dial CTV @13:26:46.05 delay 2 seconds @13:26:48.07 CSV.dial :air @13:26:48.52 delay 2 seconds @13:26:50.54 CS.seek 0ml @13:27:04.15 Puck @27.4psia after final lysis pressurization @13:27:04.16 Recovering Lysate into Collection syringe @13:27:04.17 CSV.dial CBV @13:27:05.16 CBV.dial CSV @13:27:05.55 CS.seek 5ml @13:27:19.22 delay 5 seconds @13:27:24.24 CTV.dial :air @13:27:24.77 CS.seek 7ml @13:27:30.72 delay 5 seconds @13:27:35.74 CSV.dial :air2 @13:27:36.18 CS.seek 10ml @13:27:44.72 CSV.dial between air and CTV,avoiding: CBV @13:27:45.87 Lysing Complete @13:27:45.94 Storing sh1 puck in Collection Clamp to tube 2 @13:27:46.11 SC.reconfigure :tight @13:27:46.15 SC.seek 2 @13:27:49.90 SC.configure :limp @13:27:50.11 Elbow.reconfigure :tight @13:27:50.15 Elbow.seek 2 @13:27:51.86 Forearm.reconfigure :tight @13:27:51.91 Forearm.seek 2 + 800 counts @13:27:53.09 SE.reconfigure :pick @13:27:53.14 SE.seek :beamBreak @13:27:55.63 SE.configure :default @13:27:55.78 Elbow.reconfigure :limp @13:27:55.83 Forearm.reconfigure :loose @13:27:55.88 Forearm.seek 2 @13:27:58.24 Slide::Error in MAIN -- Forearm trajectoryErr at 6 @13:27:58.25 Retry #1 of 3 @13:27:58.34 Forearm.seek :retract @13:27:59.42 Elbow.reconfigure :loose @13:27:59.91 Elbow.seek 2 @13:27:59.98 Elbow.reconfigure :limp @13:28:00.05 Forearm.seek 2 @13:28:03.08 Slide::Error in MAIN -- Forearm trajectoryErr at 2 @13:28:03.09 Retry #2 of 3 @13:28:03.18 Forearm.seek :retract @13:28:04.20 Elbow.reconfigure :loose @13:28:04.25 Elbow.seek 2 @13:28:04.32 Elbow.reconfigure :limp @13:28:04.38 Forearm.seek 2 @13:28:07.42 Slide::Error in MAIN -- Forearm trajectoryErr at 6 @13:28:07.44 Retry #3 of 3 @13:28:07.53 Forearm.seek :retract @13:28:08.53 Elbow.reconfigure :loose @13:28:08.58 Elbow.seek 2 @13:28:08.65 Elbow.reconfigure :limp @13:28:08.72 Forearm.seek 2 @13:28:11.77 Slide::Error in MAIN -- Forearm trajectoryErr at 6 @13:28:11.78 Retry #1 of 4 @13:28:11.81 SE.seek :down @13:28:14.22 Elbow.reconfigure :tight @13:28:14.27 Elbow.seek 2 @13:28:14.48 Forearm.reconfigure :tight @13:28:14.53 Forearm.seek 2 + 800 counts @13:28:14.85 SE.reconfigure :pick @13:28:14.91 SE.seek :beamBreak @13:28:17.50 SE.configure :default @13:28:17.67 Elbow.reconfigure :limp @13:28:17.73 Forearm.reconfigure :loose @13:28:17.78 Forearm.seek 2 @13:28:20.09 Slide::Error in MAIN -- Forearm trajectoryErr at 6 @13:28:20.11 Retry #1 of 3 @13:28:20.19 Forearm.seek :retract @13:28:21.19 Elbow.reconfigure :loose @13:28:21.24 Elbow.seek 2 @13:28:21.31 Elbow.reconfigure :limp @13:28:21.38 Forearm.seek 2 @13:28:24.42 Slide::Error in MAIN -- Forearm trajectoryErr at 6 @13:28:24.44 Retry #2 of 3 @13:28:24.52 Forearm.seek :retract @13:28:25.61 Elbow.reconfigure :loose @13:28:26.10 Elbow.seek 2 @13:28:26.17 Elbow.reconfigure :limp @13:28:26.24 Forearm.seek 2 @13:28:29.25 Slide::Error in MAIN -- Forearm trajectoryErr at 6 @13:28:29.27 Retry #3 of 3 @13:28:29.36 Forearm.seek :retract @13:28:30.40 Elbow.reconfigure :loose @13:28:30.45 Elbow.seek 2 @13:28:30.53 Elbow.reconfigure :limp @13:28:30.60 Forearm.seek 2 @13:28:32.32 Elbow.hold @13:28:32.40 Elbow.reconfigure :tight @13:28:32.52 SE.seek 5.08 Pucks below Top Plate @13:28:32.98 Forearm.force -20 @13:28:33.04 Forearm.seek 2 + 267 counts @13:28:33.43 Forearm.reconfigure :tight @13:28:33.48 Forearm.seek 2 + 800 counts @13:28:33.73 SE.seek 6.08 Pucks below Top Plate @13:28:34.33 Forearm.reconfigure :loose @13:28:34.37 Forearm.seek :retract @13:28:35.40 Elbow.seek CC @13:28:37.07 Elbow.reconfigure :loose @13:28:37.16 Forearm.seek CC @13:28:38.17 Hand.close @13:28:38.94 CC.seek :open @13:28:43.14 Forearm.seek :retract @13:28:44.12 Elbow.reconfigure :tight @13:28:44.17 Elbow.seek 2 @13:28:45.89 Elbow.reconfigure :limp @13:28:45.94 Forearm.seek 2 @13:28:47.09 Elbow.hold @13:28:47.15 Elbow.reconfigure :tight @13:28:47.62 Hand.open @13:28:48.56 Forearm.force -20 @13:28:48.60 Forearm.seek 2 + 319 counts @13:28:48.98 Forearm.reconfigure :tight @13:28:49.03 Forearm.seek 2 + 800 counts @13:28:49.31 SE.seek :down @13:28:50.44
Forearm.reconfigure :loose @13:28:50.49 Forearm.seek :retract @13:28:51.46 SE.stop @13:28:51.50
Tranferring DA @13:28:51.51 Beginning samplePassC @13:28:51.52 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:28:51.71 Elbow.seek FlushPuck::Garage @13:28:53.47 Elbow.reconfigure :loose @13:28:53.57 Forearm.seek :garage @13:28:54.54 Hand.close @13:28:55.43 Forearm.seek :retract @13:28:56.39 Elbow.reconfigure :tight @13:28:56.44 Elbow.seek CC @13:28:59.25 Elbow.reconfigure :loose @13:28:59.35 Forearm.reconfigure :tight @13:28:59.40 Forearm.seek CC @13:29:00.67 CC.seek :closed @13:29:05.22 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.94 @13:29:05.29 Hand.open @13:29:06.12 Forearm.reconfigure :loose @13:29:06.17 Forearm.seek :retract @13:29:07.15 PSV.dial PSR @13:29:08.66 Pulling 1.5ml of PSR.flush @13:29:08.67 PSR.select :flush @13:29:08.78 PS.seek 1.5ml @13:29:24.93 delay 5 seconds @13:29:29.95 PSV.dial :waste1 @13:29:30.44 PS.seek 0ml @13:29:46.52 PSV.dial PSR @13:29:46.99 PSR.select :air @13:29:47.11 PS.seek 2.5ml @13:30:13.46 PRV.dial CBV,avoiding: :ab2 @13:30:13.49
CBV.dial PRV @13:30:15.24 PSV.dial :mixloop @13:30:16.27 CTV.dial :air,avoiding: :intake @13:30:16.35 PTV.dial :PRVmixing @13:30:17.65 PS.seek 0ml @13:30:43.96 PSV.dial :air @13:30:44.43 PS.seek 2.5ml @13:31:10.83 PSV.dial :mixloop @13:31:11.31 PS.seek 0ml @13:31:37.68 PSV.dial :air @13:31:38.15 PS.seek 2.5ml @13:32:04.50 PSV.dial :mixloop @13:32:04.98 PS.seek 0ml @13:32:31.55 Garaging FlushPuck in Collection Clamp @13:32:31.63 Elbow.reconfigure :tight @13:32:31.67 Elbow.seek CC @13:32:31.79 Elbow.reconfigure :loose @13:32:31.89 Forearm.seek CC @13:32:32.91 Hand.close @13:32:33.68 CC.seek :open @13:32:37.77 Forearm.seek :retract @13:32:39.28 Elbow.reconfigure :tight @13:32:39.32 Elbow.seek :garage @13:32:42.21 Elbow.reconfigure :loose @13:32:42.31 Forearm.reconfigure :tight @13:32:42.36 Forearm.seek :garage @13:32:43.30 Hand.open @13:32:44.12 Forearm.reconfigure :loose @13:32:44.16 Forearm.seek :retract @13:32:45.15 PSV.dial :mixloop @13:32:45.22 PTV.dial :PRVmixing @13:32:45.24 PRV.dial CBV @13:32:45.26
CBV.dial PRV @13:32:45.48 CTV.dial :air,avoiding: :intake @13:32:45.55 Loading lyfil puck from tube 3 into Collection Clamp @13:32:45.78 SC.reconfigure :tight @13:32:45.83 SC.seek 3 @13:32:49.41 SC.configure :limp @13:32:49.62 Elbow.reconfigure :tight @13:32:49.67 Elbow.seek 3 @13:32:51.59 Forearm.reconfigure :tight @13:32:51.64 Forearm.seek 3 + 800 counts @13:32:52.63 SE.reconfigure :pick @13:32:52.69 SE.seek :beamBreak @13:32:54.38 SE.configure :default @13:32:54.55 Elbow.seek 3 @13:32:54.88 Forearm.seek 3 + 300 counts @13:32:55.23 Forearm.reconfigure :loose @13:32:55.27 Hand.close @13:32:56.07 SE.seek :down @13:32:56.71
Forearm.seek :retract @13:32:57.52 SE.stop @13:32:57.64
Elbow.seek CC @13:32:59.25 Elbow.reconfigure :loose @13:32:59.35 Forearm.reconfigure :tight @13:32:59.40 Forearm.seek CC @13:33:00.65 CC.seek :closed @13:33:04.66 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 2.01 @13:33:04.67 Hand.open @13:33:05.49 Forearm.reconfigure :loose @13:33:05.54 Forearm.seek :retract @13:33:06.52 CTV.dial CSV,avoiding: :intake @13:33:07.89 CSV.dial CTV @13:33:08.33 PS.seek 2.5ml @13:33:34.74 CS.seek 5ml @13:33:48.37 delay 8 seconds @13:33:56.40 PSV.dial :air @13:33:56.86 delay 2 seconds @13:33:58.88 PSV.dial :waste2 @13:33:59.62 PS.seek 0ml @13:34:25.99 CBV.dial between PRV and waste1 @13:34:26.33 CSV.dial :air @13:34:26.77 CS.seek 10ml @13:34:40.43 CSV.dial CTV @13:34:40.90 CS.seek 0ml @13:35:07.28 CBV.dial :waste1 @13:35:07.59 delay 5 seconds @13:35:12.62 CTV.dial :air,avoiding: :intake @13:35:14.04 Storing lyfil puck in Collection Clamp to tube 2 @13:35:14.66 SC.reconfigure :tight @13:35:14.71 SC.seek 2 @13:35:18.31 SC.configure :limp @13:35:18.53 Elbow.reconfigure :tight @13:35:18.58 Elbow.seek 2 @13:35:20.27 Forearm.reconfigure :tight @13:35:20.32 Forearm.seek 2 + 800 counts @13:35:21.40 SE.reconfigure :pick @13:35:21.45 SE.seek :beamBreak @13:35:23.85 SE.configure :default @13:35:24.01 Elbow.reconfigure :limp @13:35:24.06 Forearm.reconfigure :loose @13:35:24.11 Forearm.seek 2 @13:35:24.49 Elbow.hold @13:35:24.56 Elbow.reconfigure :tight @13:35:24.66 SE.seek 6.08 Pucks below Top Plate @13:35:25.22 Forearm.force -20 @13:35:25.25 Forearm.seek 2 + 305 counts @13:35:25.64 Forearm.reconfigure :tight @13:35:25.69 Forearm.seek 2 + 800 counts @13:35:25.90 SE.seek 7.08 Pucks below Top Plate @13:35:26.53 Forearm.reconfigure :loose @13:35:26.57 Forearm.seek :retract @13:35:27.55 Elbow.seek CC @13:35:29.25 Elbow.reconfigure :loose @13:35:29.35 Forearm.seek CC @13:35:30.38 Hand.close @13:35:31.06 CC.seek :open @13:35:35.71 Forearm.seek :retract @13:35:36.90 Elbow.reconfigure :tight @13:35:36.95 Elbow.seek 2 @13:35:38.66 Elbow.reconfigure :limp @13:35:38.71 Forearm.seek 2 @13:35:39.80 Elbow.hold @13:35:39.87 Elbow.reconfigure :tight @13:35:39.92 Hand.open @13:35:40.81 Forearm.force -20 @13:35:40.84 Forearm.seek 2 + 319 counts @13:35:41.23 Forearm.reconfigure :tight @13:35:41.28 Forearm.seek 2 + 800 counts @13:35:41.58 SE.seek :down @13:35:42.72
Forearm.reconfigure :loose @13:35:42.77 Forearm.seek :retract @13:35:43.64 SE.stop @13:35:43.76
PSV.dial :air @13:35:44.49 PS.seek 2.5ml @13:36:10.86 delay 1 second @13:36:11.88 PSV.dial :mixloop @13:36:12.33 delay 1 second @13:36:13.35 PSV.dial :air @13:36:13.82 delay 1 second @13:36:14.83 PSV.dial :mixloop @13:36:15.33 delay 1 second @13:36:16.35 PSV.dial :air @13:36:16.82 delay 1 second @13:36:17.84 PS.seek 0ml @13:36:44.18 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:36:44.36 Elbow.seek FlushPuck::Garage @13:36:46.10 Elbow.reconfigure :loose @13:36:46.64 Forearm.seek :garage @13:36:47.68 Hand.close @13:36:48.55 Forearm.seek :retract @13:36:49.56 Elbow.reconfigure :tight @13:36:49.61 Elbow.seek PC @13:36:50.90 Elbow.reconfigure :loose @13:36:51.00 Forearm.reconfigure :tight @13:36:51.05 Forearm.seek PC @13:36:52.44 PC.seek :closed @13:36:56.87 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.53 @13:36:56.93 Hand.open @13:36:57.74 Forearm.reconfigure :loose @13:36:57.79 Forearm.seek :retract @13:36:58.86 PRV.dial :air @13:36:59.33 PSV.dial :mixloop @13:36:59.87 PS.seek 0.3ml @13:37:03.80 PSV.dial PSR @13:37:04.84 Pulling 0.4ml of PSR.air @13:37:04.85 PSR.select :air @13:37:04.87 PS.seek 0.7ml @13:37:10.09 delay 3 seconds @13:37:13.11 PSR.select :air @13:37:13.13 PS.seek 2.5ml @13:37:32.42 delay 3 seconds @13:37:35.44 PSV.dial :mixloop @13:37:36.49 PTV.dial :mixing @13:37:37.22 PBV.dial :waste2 @13:37:38.21 PS.seek 0ml @13:38:04.74 Garaging FlushPuck in Processing Clamp @13:38:04.81 Elbow.reconfigure :tight @13:38:05.23 Elbow.seek PC @13:38:05.48 Elbow.reconfigure :loose @13:38:05.59 Forearm.seek PC @13:38:06.69 Hand.close @13:38:07.43 PC.seek :open @13:38:11.94 Forearm.seek :retract @13:38:12.97 Elbow.reconfigure :tight @13:38:13.02 Elbow.seek :garage @13:38:14.45 Elbow.reconfigure :loose @13:38:14.55 Forearm.reconfigure :tight @13:38:14.59 Forearm.seek :garage @13:38:15.67 Hand.open @13:38:16.49 Forearm.reconfigure :loose @13:38:16.53 Forearm.seek :retract @13:38:17.62 Loading da puck from tube 3 into Processing Clamp @13:38:17.85 SC.reconfigure :tight @13:38:17.90 SC.seek 3 @13:38:21.56 SC.configure :limp @13:38:21.77 Elbow.reconfigure :tight @13:38:21.82 Elbow.seek 3 @13:38:23.71 Forearm.reconfigure :tight @13:38:23.76 Forearm.seek 3 + 800 counts @13:38:24.80 SE.reconfigure :pick @13:38:24.86 SE.seek :beamBreak @13:38:26.58 SE.configure :default @13:38:26.73 Elbow.seek 3 @13:38:27.05 Forearm.seek 3 + 300 counts @13:38:27.22 Forearm.reconfigure :loose @13:38:27.27 Hand.close @13:38:28.44 SE.seek :down @13:38:28.72
Forearm.seek :retract @13:38:29.69 Elbow.seek PC @13:38:30.07 SE.stop @13:38:32.21
Elbow.reconfigure :loose @13:38:32.32 Forearm.reconfigure :tight @13:38:32.36 Forearm.seek PC @13:38:33.75 PC.seek :closed @13:38:38.18 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.55 @13:38:38.19 Hand.open @13:38:38.99 Forearm.reconfigure :loose @13:38:39.03 Forearm.seek :retract @13:38:40.01 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @13:38:40.03 PH.seek 28C,5:00 @13:39:29.05 PH.hold 2:00:00,28C @13:39:29.10 delay 1:30 @13:40:59.15 PSR.select :air @13:40:59.16 PSV.dial PSR @13:41:00.20 Pulling 0.5ml of PSR.wash @13:41:00.21 PSR.select :wash @13:41:00.33 PS.seek 0.5ml @13:41:06.45 delay 5 seconds @13:41:11.47 PSR.select :air @13:41:11.59 PS.seek 2ml @13:41:27.83 PTV.dial :puck @13:41:28.56 PSV.dial PTV @13:41:29.31 PBV.dial :waste1 @13:41:30.75 PS.seek 1.5ml @13:41:36.73 delay 3 seconds @13:41:39.75 PBV.dial between PSV and waste1 @13:41:40.13 PS.seek 0ml @13:41:56.33 delay 5:00 @13:46:56.35 recoverPSRreagent08 @13:46:56.36 PTV.dial :puck @13:46:56.43 PBV.dial :waste1 @13:46:56.82 delay 30 seconds @13:47:26.85 PBV.dial between PSV and waste1 @13:47:27.24 PSR.select :air @13:47:27.26 PSV.dial PSR @13:47:28.02 PS.reconfigure :fast @13:47:28.06 PS.seek 2.5ml @13:47:51.45 PSV.dial PTV @13:47:52.20 delay 5 seconds @13:47:57.22 PSV.dial PSR @13:47:57.96 delay 5 seconds @13:48:02.98 PSV.dial PTV @13:48:03.73 delay 5 seconds @13:48:09.18 PSV.dial :air @13:48:09.95 PS.seek 0ml @13:48:32.98 PTV.dial :PRVmixing,via: PRV @13:48:34.29 PSV.dial :mixloop @13:48:34.74 PRV.dial :ab1 @13:48:35.48 PS.reconfigure :slow @13:48:35.53 PS.seek 0.15ml @13:48:39.10 delay 2 seconds @13:48:41.12 CBV.dial PRV @13:48:41.16
PRV.dial CBV @13:48:41.71 PS.seek 0.34ml @13:48:46.04 delay 2 seconds @13:48:48.06 CBV.dial between PRV and PSV @13:48:48.38 PRV.dial :air @13:48:48.81 PS.seek 0.9ml @13:49:00.76 PRV.dial :waste2 @13:49:01.52 PS.seek 0.5ml @13:49:10.13 addPMLReagent08 @13:49:10.14 PSV.dial :air @13:49:10.59 PS.reconfigure :default @13:49:10.64 PS.seek 2ml @13:49:26.76 PSV.dial :PML @13:49:27.21 PTV.dial :mixing @13:49:27.95 PBV.dial :waste1 @13:49:28.40 PS.seek 1.85ml @13:49:30.79 PBV.dial between PSV and waste1 @13:49:31.17 PS.seek 0ml @13:49:50.82 delay 2 seconds @13:49:52.84 Beginning samplePassC @13:49:52.85 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:49:53.03 Elbow.reconfigure :tight @13:49:53.08 Elbow.seek FlushPuck::Garage @13:49:54.48 Elbow.reconfigure :loose @13:49:54.58 Forearm.seek :garage @13:49:55.63 Hand.close @13:49:56.53 Forearm.seek :retract @13:49:57.52 Elbow.reconfigure :tight @13:49:57.57 Elbow.seek CC @13:50:00.41 Elbow.reconfigure :loose @13:50:00.94 Forearm.reconfigure :tight @13:50:00.99 Forearm.seek CC @13:50:02.28 CC.seek :closed @13:50:06.30 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.07 @13:50:06.37 Hand.open @13:50:07.23 Forearm.reconfigure :loose @13:50:07.28 Forearm.seek :retract @13:50:08.38 PSV.dial PSR @13:50:09.43 Pulling 1.5ml of PSR.flush @13:50:09.44 PSR.select :flush @13:50:09.56 PS.seek 1.5ml @13:50:25.66 delay 5 seconds @13:50:30.68 PSV.dial :waste1 @13:50:31.14 PS.seek 0ml @13:50:47.23 PSV.dial PSR @13:50:47.69 PSR.select :air @13:50:47.81 PS.seek 2.5ml @13:51:14.20 PRV.dial CBV,avoiding: :ab2 @13:51:14.22
CBV.dial PRV @13:51:15.85 PSV.dial :mixloop @13:51:16.88 CTV.dial :air,avoiding: :intake @13:51:16.96 PTV.dial :PRVmixing @13:51:17.72 PS.seek 0ml @13:51:44.07 PSV.dial :air @13:51:44.53 PS.seek 2.5ml @13:52:11.07 PSV.dial :mixloop @13:52:11.54 PS.seek 0ml @13:52:37.85 PSV.dial :air @13:52:38.32 PS.seek 2.5ml @13:53:04.66 PSV.dial :mixloop @13:53:05.14 PS.seek 0ml @13:53:31.94 Garaging FlushPuck in Collection Clamp @13:53:32.02 Elbow.reconfigure :tight @13:53:32.07 Elbow.seek CC @13:53:32.23 Elbow.reconfigure :loose @13:53:32.33 Forearm.seek CC @13:53:33.28 Hand.close @13:53:34.04 CC.seek :open @13:53:38.13 Forearm.seek :retract @13:53:39.18 Elbow.reconfigure :tight @13:53:39.23 Elbow.seek :garage @13:53:42.09 Elbow.reconfigure :loose @13:53:42.19 Forearm.reconfigure :tight @13:53:42.23 Forearm.seek :garage @13:53:43.34 Hand.open @13:53:44.10 Forearm.reconfigure :loose @13:53:44.14 Forearm.seek :retract @13:53:45.12 delayUntil "13:57:52.83" @13:53:45.14 Whole Cell Archive sampling 1000ml @13:53:45.16 Loading wcr puck from tube 3 into Collection Clamp @13:53:45.39 SC.reconfigure :tight @13:53:45.44 SC.seek 3 @13:53:45.51 SC.configure :limp @13:53:45.73 Elbow.reconfigure :tight @13:53:45.79 Elbow.seek 3 @13:53:47.69 Forearm.reconfigure :tight @13:53:47.74 Forearm.seek 3 + 800 counts @13:53:48.79 SE.reconfigure :pick @13:53:48.85 SE.seek :beamBreak @13:53:50.75 SE.configure :default @13:53:51.36 Elbow.seek 3 @13:53:51.55 Forearm.seek 3 + 300 counts @13:53:51.72 Forearm.reconfigure :loose @13:53:51.77 Hand.close @13:53:52.55 SE.seek :down @13:53:52.75 Forearm.seek :retract @13:53:53.86 Elbow.seek CC @13:53:54.22 SE.stop @13:53:55.43 Elbow.reconfigure :loose @13:53:55.54 Forearm.reconfigure :tight @13:53:55.59 Forearm.seek CC @13:53:56.92 CC.seek :closed @13:54:00.90 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.86 @13:54:00.91 Hand.open @13:54:01.72 Forearm.reconfigure :loose @13:54:01.77 Forearm.seek :retract @13:54:02.77 collectSample 1000ml w/bubble point of 35psi @13:54:02.78 Priming 7ml @13:54:02.80 Intake.select :close @13:54:02.81 Exhaust.select :close @13:54:02.82 SS.hold @13:54:03.02 Calibrating pressure sensors @13:54:03.04 CTV.dial :air @13:54:03.11 SSV.dial :spit,avoiding: :exhaust @13:54:03.13 SS.reconfigure :fast @13:54:03.18 SS.seek 5ml @13:54:06.09 SSV.dial CBV @13:54:06.12 CBV.dial SSV @13:54:07.97 delay 3.8 seconds @13:54:11.90 Sensor limits: Intake max 106.0psia; Outlet max 111.2psia @13:54:11.92 SSV.dial :spit @13:54:13.29 SS.seek 0ml @13:54:16.09 SSV.dial CBV @13:54:17.44 CTV.dial :intake @13:54:18.20 SSV.dial CBV @13:54:18.23 CBV.dial SSV @13:54:18.47 Allowing 40s for 7.0ml of remaining 7.0ml @13:54:18.48 Intake.select :open @13:54:20.65 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:54:20.67 SS.reconfigure :inhaling 2.17ml/s @13:54:20.72 SS.seek 7ml @13:54:26.73 Stroke completed -- waiting for pressure to equalize ... @13:54:26.74 SS.reconfigure :exhale @13:54:26.79 SS.hold @13:54:36.56 Intake.select :close @13:54:37.11 SSV.dial :exhaust @13:54:37.97 Exhaust.select :open @13:54:38.08 SS.seek 0ml @13:54:42.43 Exhaust.select :close @13:54:42.55 Sampling 1000.0ml @13:54:42.56 Intake.select :close @13:54:42.57 Exhaust.select :close @13:54:42.59 SS.reconfigure :default @13:54:42.63 SS.hold @13:54:42.85 CTV.dial :intake @13:54:42.87 SSV.dial CBV @13:54:42.90 CBV.dial SSV @13:54:43.70 Allowing 130s for 25.0ml of remaining 1000.0ml @13:54:43.71 Intake.select :open @13:54:45.87 Intake @13.8psia, vacuum from 8.3 to 11.3psi @13:54:45.89 SS.reconfigure :inhaling 2.17ml/s @13:54:45.94 SS.seek 25ml @13:54:53.61 Vacuum too high -- waiting up to 40 seconds @13:54:53.63 SS.reconfigure :exhale @13:54:53.68 SS.hold @13:55:02.64 Intake @12.2psia, vacuum from 6.7 to 9.7psi @13:55:02.66 SS.reconfigure :inhaling 1.95ml/s @13:55:03.17 SS.seek 25ml @13:55:07.18 Vacuum too high -- waiting up to 40 seconds @13:55:07.19 SS.reconfigure :exhale @13:55:07.25 SS.hold @13:55:14.18 Intake @12.1psia, vacuum from 6.6 to 9.6psi @13:55:14.20 SS.reconfigure :inhaling 1.77ml/s @13:55:14.25 SS.seek 25ml @13:55:19.02 Vacuum too high -- waiting up to 40 seconds @13:55:19.04 SS.reconfigure :exhale @13:55:19.10 SS.hold @13:55:26.34 Intake @12.2psia, vacuum from 6.7 to 9.7psi @13:55:26.36 SS.reconfigure :inhaling 1.59ml/s @13:55:26.41 SS.seek 25ml @13:55:26.96 Stroke completed -- waiting for pressure to equalize ... @13:55:26.98 SS.reconfigure :exhale @13:55:27.02 SS.hold @13:55:29.52 Intake.select :close @13:55:29.68 SSV.dial :exhaust @13:55:30.45 Exhaust.select :open @13:55:30.56 SS.seek 0ml @13:55:39.87 Exhaust.select :close @13:55:39.98 SSV.dial CBV @13:55:40.01 CBV.dial SSV @13:55:40.81 Allowing 130s for 25.0ml of remaining 975.0ml @13:55:40.82 Intake.select :open @13:55:42.99 Intake @13.5psia, vacuum from 8.0 to 11.0psi @13:55:43.01 SS.reconfigure :inhaling 1.59ml/s @13:55:43.06 SS.seek 25ml @13:55:50.02 Vacuum too high -- waiting up to 40 seconds @13:55:50.03 SS.reconfigure :exhale @13:55:50.09 SS.hold @13:55:56.13 Intake @12.3psia, vacuum from 6.8 to 9.8psi @13:55:56.15 SS.reconfigure :inhaling 1.43ml/s @13:55:56.20 SS.seek 25ml @13:56:01.45 Vacuum too high -- waiting up to 40 seconds @13:56:01.47 SS.reconfigure :exhale @13:56:01.52 SS.hold @13:56:08.28 Intake @12.2psia, vacuum from 6.7 to 9.7psi @13:56:08.30 SS.reconfigure :inhaling 1.29ml/s @13:56:08.35 SS.seek 25ml @13:56:14.89 Vacuum too high -- waiting up to 40 seconds @13:56:14.91 SS.reconfigure :exhale @13:56:14.96 SS.hold @13:56:21.19 Intake @12.0psia, vacuum from 6.5 to 9.5psi @13:56:21.21 SS.reconfigure :inhaling 1.16ml/s @13:56:21.26 SS.seek 25ml @13:56:24.02 Stroke completed -- waiting for pressure to equalize ... @13:56:24.04 SS.reconfigure :exhale @13:56:24.08 SS.hold @13:56:26.56 Intake.select :close @13:56:26.72 SSV.dial :exhaust @13:56:27.49 Exhaust.select :open @13:56:27.61 SS.seek 0ml @13:56:36.96 Exhaust.select :close @13:56:37.07 SSV.dial CBV @13:56:37.11 CBV.dial SSV @13:56:37.87 Allowing 130s for 25.0ml of remaining 950.0ml @13:56:37.88 Intake.select :open @13:56:40.45 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:56:40.47 SS.reconfigure :inhaling 1.16ml/s @13:56:40.52 SS.seek 25ml @13:56:51.05 Vacuum too high -- waiting up to 40 seconds @13:56:51.06 SS.reconfigure :exhale @13:56:51.12 SS.hold @13:56:54.77 Intake @12.3psia, vacuum from 6.8 to 9.8psi @13:56:54.79 SS.reconfigure :inhaling 1.04ml/s @13:56:54.84 SS.seek 25ml @13:57:02.14 Vacuum too high -- waiting up to 40 seconds @13:57:02.16 SS.reconfigure :exhale @13:57:02.21 SS.hold @13:57:08.42 Intake @12.2psia, vacuum from 6.7 to 9.7psi @13:57:08.44 SS.reconfigure :inhaling 0.94ml/s @13:57:08.49 SS.seek 25ml @13:57:16.93 Stroke completed -- waiting for pressure to equalize ... @13:57:16.94 SS.reconfigure :exhale @13:57:16.99 SS.hold @13:57:23.49 Intake.select :close @13:57:23.65 SSV.dial :exhaust @13:57:24.43 Exhaust.select :open @13:57:24.54 SS.seek 0ml @13:57:33.54 Exhaust.select :close @13:57:33.65 SSV.dial CBV @13:57:33.67 CBV.dial SSV @13:57:34.48 Allowing 130s for 25.0ml of remaining 925.0ml @13:57:34.49 Intake.select :open @13:57:36.66 Intake @13.7psia, vacuum from 8.2 to 11.2psi @13:57:36.68 SS.reconfigure :inhaling 0.94ml/s @13:57:36.73 SS.seek 25ml @13:57:52.61 Vacuum too high -- waiting up to 40 seconds @13:57:52.63 SS.reconfigure :exhale @13:57:52.68 SS.hold @13:57:52.85 recoverPRVreagent08 @13:57:52.87 PTV.dial :mixing @13:57:53.81 PBV.dial :waste1 @13:57:54.19 delay 30 seconds @13:57:57.32 Intake @12.2psia, vacuum from 6.7 to 9.7psi @13:57:57.34 SS.reconfigure :inhaling 0.85ml/s @13:57:57.39 SS.seek 25ml @13:58:11.74 Stroke completed -- waiting for pressure to equalize ... @13:58:11.75 SS.reconfigure :exhale @13:58:11.80 SS.hold @13:58:20.36 Intake.select :close @13:58:20.99 SSV.dial :exhaust @13:58:21.77 Exhaust.select :open @13:58:21.89 SS.seek 0ml @13:58:24.21 PBV.dial between PSV and waste1 @13:58:24.58 PSV.dial :air @13:58:25.05 PS.reconfigure :fast @13:58:25.10 PS.seek 2.5ml @13:58:31.38 Exhaust.select :close @13:58:31.50 SSV.dial CBV @13:58:31.53 CBV.dial SSV @13:58:32.32 Allowing 130s for 25.0ml of remaining 900.0ml @13:58:32.33 Intake.select :open @13:58:34.50 Intake @13.9psia, vacuum from 8.4 to 11.4psi @13:58:34.52 SS.reconfigure :inhaling 0.85ml/s @13:58:34.57 SS.seek 25ml @13:58:48.78 PSV.dial :mixloop @13:58:49.25 delay 2 seconds @13:58:51.27 PSV.dial :air @13:58:51.74 delay 2 seconds @13:58:52.61 Vacuum too high -- waiting up to 40 seconds @13:58:52.63 SS.reconfigure :exhale @13:58:52.68 SS.hold @13:58:53.75 PSV.dial :mixloop @13:58:54.22 delay 2 seconds @13:58:56.33 PSV.dial :air @13:58:56.40 Intake @12.3psia, vacuum from 6.8 to 9.8psi @13:58:56.42 SS.reconfigure :inhaling 0.77ml/s @13:58:56.46 SS.seek 25ml @13:58:56.80 PS.seek 0ml @13:59:11.43 Stroke completed -- waiting for pressure to equalize ... @13:59:11.44 SS.reconfigure :exhale @13:59:11.49 SS.hold @13:59:19.34 Intake.select :close @13:59:19.50 SSV.dial :exhaust @13:59:20.06 washCycleDA #1 of 2 @13:59:20.08 PSR.select :air @13:59:20.09 PSV.dial PSR @13:59:20.29 Exhaust.select :open @13:59:20.41 SS.seek 0ml @13:59:21.42 Pulling 0.5ml of PSR.wash @13:59:21.43 PSR.select :wash @13:59:21.55 PS.reconfigure :default @13:59:21.60 PS.seek 0.5ml @13:59:27.59 delay 5 seconds @13:59:29.79 Exhaust.select :close @13:59:29.90 SSV.dial CBV @13:59:29.92 CBV.dial SSV @13:59:30.72 Allowing 130s for 25.0ml of remaining 875.0ml @13:59:30.74 Intake.select :open @13:59:32.61 PSR.select :air @13:59:32.73 PS.seek 2ml @13:59:32.90 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:59:32.92 SS.reconfigure :inhaling 0.77ml/s @13:59:32.97 SS.seek 25ml @13:59:48.91 PTV.dial :puck @13:59:49.64 PSV.dial PTV @13:59:50.39 PBV.dial :waste1 @13:59:50.83 PS.seek 1.5ml @13:59:56.90 delay 3 seconds @13:59:59.92 PBV.dial between PSV and waste1 @14:00:00.30 PS.seek 0ml @14:00:04.45 Vacuum too high -- waiting up to 40 seconds @14:00:04.47 SS.reconfigure :exhale @14:00:04.52 SS.hold @14:00:07.61 Intake @12.4psia, vacuum from 6.9 to 9.9psi @14:00:07.63 SS.reconfigure :inhaling 0.70ml/s @14:00:07.68 SS.seek 25ml @14:00:11.33 Stroke completed -- waiting for pressure to equalize ... @14:00:11.34 SS.reconfigure :exhale @14:00:11.39 SS.hold @14:00:16.38 delay 2:00 @14:00:18.29 Intake.select :close @14:00:18.51 SSV.dial :exhaust @14:00:19.30 Exhaust.select :open @14:00:19.42 SS.seek 0ml @14:00:28.38 Exhaust.select :close @14:00:28.49 SSV.dial CBV @14:00:28.53 CBV.dial SSV @14:00:29.32 Allowing 130s for 25.0ml of remaining 850.0ml @14:00:29.33 Intake.select :open @14:00:31.49 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:00:31.51 SS.reconfigure :inhaling 0.70ml/s @14:00:31.56 SS.seek 25ml @14:01:09.46 Stroke completed -- waiting for pressure to equalize ... @14:01:09.47 SS.reconfigure :exhale @14:01:09.52 SS.hold @14:01:16.99 Intake.select :close @14:01:17.15 SSV.dial :exhaust @14:01:17.93 Exhaust.select :open @14:01:18.04 SS.seek 0ml @14:01:27.46 Exhaust.select :close @14:01:27.57 SSV.dial CBV @14:01:27.60 CBV.dial SSV @14:01:28.85 Allowing 130s for 25.0ml of remaining 825.0ml @14:01:28.86 Intake.select :open @14:01:31.03 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:01:31.05 SS.reconfigure :inhaling 0.70ml/s @14:01:31.10 SS.seek 25ml @14:02:08.41 Stroke completed -- waiting for pressure to equalize ... @14:02:08.42 SS.reconfigure :exhale @14:02:08.47 SS.hold @14:02:16.40 recoverPSRreagent08 @14:02:16.41 PTV.dial :puck @14:02:16.48 PBV.dial :waste1 @14:02:16.86 delay 30 seconds @14:02:18.27 Intake.select :close @14:02:18.43 SSV.dial :exhaust @14:02:19.20 Exhaust.select :open @14:02:19.31 SS.seek 0ml @14:02:28.25 Exhaust.select :close @14:02:28.36 SSV.dial CBV @14:02:28.39 CBV.dial SSV @14:02:29.26 Allowing 130s for 25.0ml of remaining 800.0ml @14:02:29.27 Intake.select :open @14:02:31.44 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:02:31.46 SS.reconfigure :inhaling 0.70ml/s @14:02:31.51 SS.seek 25ml @14:02:46.88 PBV.dial between PSV and waste1 @14:02:47.25 PSR.select :air @14:02:47.27 PSV.dial PSR @14:02:48.14 PS.reconfigure :fast @14:02:48.20 PS.seek 2.5ml @14:03:09.05 Stroke completed -- waiting for pressure to equalize ... @14:03:09.06 SS.reconfigure :exhale @14:03:09.11 SS.hold @14:03:11.68 PSV.dial PTV @14:03:12.42 delay 5 seconds @14:03:17.44 PSV.dial PSR @14:03:18.19 delay 5 seconds @14:03:18.90 Intake.select :close @14:03:19.06 SSV.dial :exhaust @14:03:19.85 Exhaust.select :open @14:03:19.97 SS.seek 0ml @14:03:23.20 PSV.dial PTV @14:03:24.42 delay 5 seconds @14:03:29.39 Exhaust.select :close @14:03:29.45 PSV.dial :air @14:03:29.51 SSV.dial CBV @14:03:29.54 CBV.dial SSV @14:03:30.22 PS.seek 0ml @14:03:30.35 Allowing 130s for 25.0ml of remaining 775.0ml @14:03:30.36 Intake.select :open @14:03:32.52 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:03:32.54 SS.reconfigure :inhaling 0.70ml/s @14:03:32.59 SS.seek 25ml @14:03:53.53 washCycleDA #2 of 2 @14:03:53.54 PSR.select :air @14:03:53.56 PSV.dial PSR @14:03:54.89 Pulling 0.5ml of PSR.wash @14:03:54.90 PSR.select :wash @14:03:55.02 PS.reconfigure :default @14:03:55.07 PS.seek 0.5ml @14:04:00.97 delay 5 seconds @14:04:05.99 PSR.select :air @14:04:06.10 Vacuum too high -- waiting up to 40 seconds @14:04:06.12 SS.reconfigure :exhale @14:04:06.18 SS.hold @14:04:06.24 PS.seek 2ml @14:04:10.34 Intake @12.8psia, vacuum from 7.3 to 10.3psi @14:04:10.36 SS.reconfigure :inhaling 0.62ml/s @14:04:10.40 SS.seek 25ml @14:04:15.20 Stroke completed -- waiting for pressure to equalize ... @14:04:15.21 SS.reconfigure :exhale @14:04:15.26 SS.hold @14:04:21.18 Intake.select :close @14:04:21.34 SSV.dial :exhaust @14:04:22.11 Exhaust.select :open @14:04:22.22 SS.seek 0ml @14:04:22.40 PTV.dial :puck @14:04:22.42 PSV.dial PTV @14:04:23.15 PBV.dial :waste1 @14:04:23.59 PS.seek 1.5ml @14:04:29.56 delay 3 seconds @14:04:31.27 Exhaust.select :close @14:04:31.38 SSV.dial CBV @14:04:31.42 CBV.dial SSV @14:04:32.19 Allowing 130s for 25.0ml of remaining 750.0ml @14:04:32.20 Intake.select :open @14:04:32.58 PBV.dial between PSV and waste1 @14:04:32.96 PS.seek 0ml @14:04:34.36 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:04:34.38 SS.reconfigure :inhaling 0.62ml/s @14:04:34.43 SS.seek 25ml @14:04:49.09 delay 2:00 @14:05:16.37 Stroke completed -- waiting for pressure to equalize ... @14:05:16.39 SS.reconfigure :exhale @14:05:16.43 SS.hold @14:05:25.47 Intake.select :close @14:05:25.63 SSV.dial :exhaust @14:05:26.39 Exhaust.select :open @14:05:26.50 SS.seek 0ml @14:05:35.84 Exhaust.select :close @14:05:35.96 SSV.dial CBV @14:05:35.98 CBV.dial SSV @14:05:36.78 Allowing 130s for 25.0ml of remaining 725.0ml @14:05:36.79 Intake.select :open @14:05:38.96 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:05:38.98 SS.reconfigure :inhaling 0.62ml/s @14:05:39.03 SS.seek 25ml @14:06:20.71 Stroke completed -- waiting for pressure to equalize ... @14:06:20.73 SS.reconfigure :exhale @14:06:20.78 SS.hold @14:06:30.55 Intake.select :close @14:06:30.71 SSV.dial :exhaust @14:06:31.50 Exhaust.select :open @14:06:31.62 SS.seek 0ml @14:06:41.00 Exhaust.select :close @14:06:41.11 SSV.dial CBV @14:06:41.14 CBV.dial SSV @14:06:41.93 Allowing 130s for 25.0ml of remaining 700.0ml @14:06:41.95 Intake.select :open @14:06:44.12 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:06:44.14 SS.reconfigure :inhaling 0.62ml/s @14:06:44.19 SS.seek 25ml @14:06:49.11 recoverPSRreagent08 @14:06:49.12 PTV.dial :puck @14:06:49.13 PBV.dial :waste1 @14:06:49.51 delay 30 seconds @14:07:19.54 PBV.dial between PSV and waste1 @14:07:19.93 PSR.select :air @14:07:19.95 PSV.dial PSR @14:07:20.70 PS.reconfigure :fast @14:07:20.75 PS.seek 2.5ml @14:07:26.04 Stroke completed -- waiting for pressure to equalize ... @14:07:26.05 SS.reconfigure :exhale @14:07:26.10 SS.hold @14:07:37.74 Intake.select :close @14:07:37.89 SSV.dial :exhaust @14:07:38.68 Exhaust.select :open @14:07:38.80 SS.seek 0ml @14:07:44.21 PSV.dial PTV @14:07:44.98 delay 5 seconds @14:07:48.28 Exhaust.select :close @14:07:48.39 SSV.dial CBV @14:07:48.41 CBV.dial SSV @14:07:49.22 Allowing 130s for 25.0ml of remaining 675.0ml @14:07:49.24 Intake.select :open @14:07:50.00 PSV.dial PSR @14:07:50.75 delay 5 seconds @14:07:51.40 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:07:51.42 SS.reconfigure :inhaling 0.62ml/s @14:07:51.47 SS.seek 25ml @14:07:55.76 PSV.dial PTV @14:07:56.51 delay 5 seconds @14:08:01.53 PSV.dial :air @14:08:02.31 PS.seek 0ml @14:08:25.93 getPMLReagent08 @14:08:25.94 PRV.dial :air @14:08:26.40 delay 5 seconds @14:08:31.42 PTV.dial :PRVmixing @14:08:32.71 delay 5 seconds @14:08:33.52 Stroke completed -- waiting for pressure to equalize ... @14:08:33.54 SS.reconfigure :exhale @14:08:33.59 SS.hold @14:08:37.73 PRV.dial :ab2 @14:08:38.22 PTV.dial :PRVmixing @14:08:38.31 PSV.dial :mixloop @14:08:38.78 PS.reconfigure :default @14:08:38.83 PS.seek 0.3ml @14:08:42.74 delay 2 seconds @14:08:44.76 PRV.dial :air @14:08:45.38 PS.seek 0.55ml @14:08:46.81 Intake.select :close @14:08:46.98 SSV.dial :exhaust @14:08:47.75 Exhaust.select :open @14:08:47.87 SS.seek 0ml @14:08:48.75 addPMLReagent08 @14:08:48.76 PSV.dial :air @14:08:49.23 PS.seek 2ml @14:08:57.26 Exhaust.select :close @14:08:57.37 SSV.dial CBV @14:08:57.40 CBV.dial SSV @14:08:58.20 Allowing 130s for 25.0ml of remaining 650.0ml @14:08:58.21 Intake.select :open @14:09:00.38 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:09:00.40 SS.reconfigure :inhaling 0.62ml/s @14:09:00.45 SS.seek 25ml @14:09:04.93 PSV.dial :PML @14:09:05.40 PTV.dial :mixing @14:09:06.16 PBV.dial :waste1 @14:09:06.62 PS.seek 1.85ml @14:09:08.93 PBV.dial between PSV and waste1 @14:09:09.31 PS.seek 0ml @14:09:28.96 delay 2 seconds @14:09:30.98 delay 8:00 @14:09:35.91 Vacuum too high -- waiting up to 40 seconds @14:09:35.93 SS.reconfigure :exhale @14:09:35.98 SS.hold @14:09:41.74 Intake @13.5psia, vacuum from 8.0 to 11.0psi @14:09:41.76 SS.reconfigure :inhaling 0.56ml/s @14:09:41.80 SS.seek 25ml @14:09:49.48 Stroke completed -- waiting for pressure to equalize ... @14:09:49.49 SS.reconfigure :exhale @14:09:49.54 SS.hold @14:09:57.17 Intake.select :close @14:09:57.33 SSV.dial :exhaust @14:09:58.10 Exhaust.select :open @14:09:58.22 SS.seek 0ml @14:10:07.62 Exhaust.select :close @14:10:07.73 SSV.dial CBV @14:10:07.77 CBV.dial SSV @14:10:08.53 Allowing 130s for 25.0ml of remaining 625.0ml @14:10:08.54 Intake.select :open @14:10:10.71 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:10:10.73 SS.reconfigure :inhaling 0.56ml/s @14:10:10.77 SS.seek 25ml @14:10:56.90 Stroke completed -- waiting for pressure to equalize ... @14:10:56.91 SS.reconfigure :exhale @14:10:56.96 SS.hold @14:11:10.10 Intake.select :close @14:11:10.26 SSV.dial :exhaust @14:11:11.02 Exhaust.select :open @14:11:11.14 SS.seek 0ml @14:11:20.52 Exhaust.select :close @14:11:20.64 SSV.dial CBV @14:11:20.66 CBV.dial SSV @14:11:21.45 Allowing 130s for 25.0ml of remaining 600.0ml @14:11:21.46 Intake.select :open @14:11:23.62 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:11:23.64 SS.reconfigure :inhaling 0.56ml/s @14:11:23.69 SS.seek 25ml @14:12:05.06 Vacuum too high -- waiting up to 40 seconds @14:12:05.08 SS.reconfigure :exhale @14:12:05.13 SS.hold @14:12:10.93 Intake @13.7psia, vacuum from 8.2 to 11.2psi @14:12:10.95 SS.reconfigure :inhaling 0.51ml/s @14:12:11.00 SS.seek 25ml @14:12:16.15 Stroke completed -- waiting for pressure to equalize ... @14:12:16.16 SS.reconfigure :exhale @14:12:16.21 SS.hold @14:12:24.78 Intake.select :close @14:12:24.95 SSV.dial :exhaust @14:12:25.71 Exhaust.select :open @14:12:25.82 SS.seek 0ml @14:12:34.86 Exhaust.select :close @14:12:34.98 SSV.dial CBV @14:12:35.00 CBV.dial SSV @14:12:35.79 Allowing 130s for 25.0ml of remaining 575.0ml @14:12:35.80 Intake.select :open @14:12:37.96 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:12:37.98 SS.reconfigure :inhaling 0.51ml/s @14:12:38.03 SS.seek 25ml @14:13:29.11 Stroke completed -- waiting for pressure to equalize ... @14:13:29.12 SS.reconfigure :exhale @14:13:29.17 SS.hold @14:13:44.01 Intake.select :close @14:13:44.17 SSV.dial :exhaust @14:13:44.96 Exhaust.select :open @14:13:45.08 SS.seek 0ml @14:13:54.54 Exhaust.select :close @14:13:54.65 SSV.dial CBV @14:13:54.67 CBV.dial SSV @14:13:55.46 Allowing 130s for 25.0ml of remaining 550.0ml @14:13:55.47 Intake.select :open @14:13:57.63 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:13:57.65 SS.reconfigure :inhaling 0.51ml/s @14:13:57.70 SS.seek 25ml @14:14:48.58 Stroke completed -- waiting for pressure to equalize ... @14:14:48.59 SS.reconfigure :exhale @14:14:48.64 SS.hold @14:15:04.07 Intake.select :close @14:15:04.23 SSV.dial :exhaust @14:15:05.02 Exhaust.select :open @14:15:05.13 SS.seek 0ml @14:15:14.63 Exhaust.select :close @14:15:14.74 SSV.dial CBV @14:15:14.78 CBV.dial SSV @14:15:15.55 Allowing 130s for 25.0ml of remaining 525.0ml @14:15:15.56 Intake.select :open @14:15:17.73 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:15:17.75 SS.reconfigure :inhaling 0.51ml/s @14:15:17.79 SS.seek 25ml @14:16:00.93 Vacuum too high -- waiting up to 40 seconds @14:16:00.95 SS.reconfigure :exhale @14:16:01.41 SS.hold @14:16:07.21 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:16:07.23 SS.reconfigure :inhaling 0.46ml/s @14:16:07.28 SS.seek 25ml @14:16:15.89 Stroke completed -- waiting for pressure to equalize ... @14:16:15.90 SS.reconfigure :exhale @14:16:15.95 SS.hold @14:16:27.61 Intake.select :close @14:16:27.78 SSV.dial :exhaust @14:16:28.55 Exhaust.select :open @14:16:28.66 SS.seek 0ml @14:16:37.64 Exhaust.select :close @14:16:37.75 SSV.dial CBV @14:16:37.77 CBV.dial SSV @14:16:38.56 Allowing 130s for 25.0ml of remaining 500.0ml @14:16:38.58 Intake.select :open @14:16:40.74 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:16:40.76 SS.reconfigure :inhaling 0.46ml/s @14:16:40.81 SS.seek 25ml @14:17:31.01 recoverPRVreagent08 @14:17:31.02 PTV.dial :mixing @14:17:31.21 PBV.dial :waste1 @14:17:31.58 delay 30 seconds @14:17:36.66 Stroke completed -- waiting for pressure to equalize ... @14:17:36.67 SS.reconfigure :exhale @14:17:36.72 SS.hold @14:17:57.14 Intake.select :close @14:17:57.30 SSV.dial :exhaust @14:17:58.07 Exhaust.select :open @14:17:58.18 SS.seek 0ml @14:18:01.60 PBV.dial between PSV and waste1 @14:18:01.99 PSV.dial :air @14:18:02.51 PS.reconfigure :fast @14:18:02.56 PS.seek 2.5ml @14:18:07.75 Exhaust.select :close @14:18:07.86 SSV.dial CBV @14:18:07.88 CBV.dial SSV @14:18:08.67 Allowing 130s for 25.0ml of remaining 475.0ml @14:18:08.69 Intake.select :open @14:18:10.86 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:18:10.87 SS.reconfigure :inhaling 0.46ml/s @14:18:10.92 SS.seek 25ml @14:18:26.30 PSV.dial :mixloop @14:18:26.77 delay 2 seconds @14:18:28.79 PSV.dial :air @14:18:29.25 delay 2 seconds @14:18:31.27 PSV.dial :mixloop @14:18:31.74 delay 2 seconds @14:18:33.75 PSV.dial :air @14:18:34.23 PS.seek 0ml @14:18:57.58 washCycleDA #1 of 3 @14:18:57.59 PSR.select :air @14:18:57.61 PSV.dial PSR @14:18:58.94 Pulling 0.5ml of PSR.wash @14:18:58.95 PSR.select :wash @14:18:59.07 PS.reconfigure :default @14:18:59.11 PS.seek 0.5ml @14:19:02.59 Vacuum too high -- waiting up to 40 seconds @14:19:02.61 SS.reconfigure :exhale @14:19:02.66 SS.hold @14:19:05.04 delay 5 seconds @14:19:10.09 PSR.select :air @14:19:10.20 PS.seek 2ml @14:19:13.00 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:19:13.02 SS.reconfigure :inhaling 0.42ml/s @14:19:13.07 SS.seek 25ml @14:19:17.89 Stroke completed -- waiting for pressure to equalize ... @14:19:17.91 SS.reconfigure :exhale @14:19:17.95 SS.hold @14:19:26.36 PTV.dial :puck @14:19:27.11 PSV.dial PTV @14:19:27.97 PBV.dial :waste1 @14:19:28.41 PS.seek 1.5ml @14:19:30.71 Intake.select :close @14:19:30.86 SSV.dial :exhaust @14:19:31.62 Exhaust.select :open @14:19:31.74 SS.seek 0ml @14:19:34.40 delay 3 seconds @14:19:37.42 PBV.dial between PSV and waste1 @14:19:37.80 PS.seek 0ml @14:19:41.22 Exhaust.select :close @14:19:41.33 SSV.dial CBV @14:19:41.37 CBV.dial SSV @14:19:42.14 Allowing 130s for 25.0ml of remaining 450.0ml @14:19:42.15 Intake.select :open @14:19:44.31 Intake @15.6psia, vacuum from 10.0 to 13.0psi @14:19:44.33 SS.reconfigure :inhaling 0.42ml/s @14:19:44.38 SS.seek 25ml @14:19:53.99 delay 2:00 @14:20:39.71 Vacuum too high -- waiting up to 40 seconds @14:20:39.73 SS.reconfigure :exhale @14:20:39.78 SS.hold @14:20:48.58 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:20:48.60 SS.reconfigure :inhaling 0.38ml/s @14:20:48.65 SS.seek 25ml @14:20:56.17 Stroke completed -- waiting for pressure to equalize ... @14:20:56.18 SS.reconfigure :exhale @14:20:56.23 SS.hold @14:21:09.41 Intake.select :close @14:21:09.57 SSV.dial :exhaust @14:21:10.34 Exhaust.select :open @14:21:10.45 SS.seek 0ml @14:21:19.93 Exhaust.select :close @14:21:20.05 SSV.dial CBV @14:21:20.07 CBV.dial SSV @14:21:20.86 Allowing 130s for 25.0ml of remaining 425.0ml @14:21:20.87 Intake.select :open @14:21:23.03 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:21:23.05 SS.reconfigure :inhaling 0.38ml/s @14:21:23.10 SS.seek 25ml @14:21:54.01 recoverPSRreagent08 @14:21:54.03 PTV.dial :puck @14:21:54.04 PBV.dial :waste1 @14:21:54.41 delay 30 seconds @14:22:24.43 PBV.dial between PSV and waste1 @14:22:24.83 PSR.select :air @14:22:24.85 PSV.dial PSR @14:22:25.72 PS.reconfigure :fast @14:22:25.77 PS.seek 2.5ml @14:22:31.88 Stroke completed -- waiting for pressure to equalize ... @14:22:31.90 SS.reconfigure :exhale @14:22:31.94 SS.hold @14:22:49.67 PSV.dial PTV @14:22:50.42 delay 5 seconds @14:22:55.44 PSV.dial PSR @14:22:56.20 delay 5 seconds @14:23:01.22 PSV.dial PTV @14:23:02.00 delay 5 seconds @14:23:02.62 Intake.select :close @14:23:02.77 SSV.dial :exhaust @14:23:03.54 Exhaust.select :open @14:23:03.66 SS.seek 0ml @14:23:07.02 PSV.dial :air @14:23:07.80 PS.seek 0ml @14:23:13.17 Exhaust.select :close @14:23:13.28 SSV.dial CBV @14:23:13.32 CBV.dial SSV @14:23:14.09 Allowing 130s for 25.0ml of remaining 400.0ml @14:23:14.10 Intake.select :open @14:23:16.26 Intake @15.9psia, vacuum from 10.0 to 13.0psi @14:23:16.28 SS.reconfigure :inhaling 0.38ml/s @14:23:16.33 SS.seek 25ml @14:23:31.87 washCycleDA #2 of 3 @14:23:31.88 PSR.select :air @14:23:31.90 PSV.dial PSR @14:23:33.25 Pulling 0.5ml of PSR.wash @14:23:33.26 PSR.select :wash @14:23:33.38 PS.reconfigure :default @14:23:33.43 PS.seek 0.5ml @14:23:39.39 delay 5 seconds @14:23:44.41 PSR.select :air @14:23:44.53 PS.seek 2ml @14:24:00.89 PTV.dial :puck @14:24:00.90 PSV.dial PTV @14:24:01.65 PBV.dial :waste1 @14:24:02.11 PS.seek 1.5ml @14:24:08.08 delay 3 seconds @14:24:11.10 PBV.dial between PSV and waste1 @14:24:11.49 PS.seek 0ml @14:24:25.26 Stroke completed -- waiting for pressure to equalize ... @14:24:25.27 SS.reconfigure :exhale @14:24:25.32 SS.hold @14:24:27.58 delay 2:00 @14:24:47.78 Intake.select :close @14:24:47.93 SSV.dial :exhaust @14:24:48.70 Exhaust.select :open @14:24:48.81 SS.seek 0ml @14:24:58.32 Exhaust.select :close @14:24:58.43 SSV.dial CBV @14:24:58.45 CBV.dial SSV @14:24:59.24 Allowing 130s for 25.0ml of remaining 375.0ml @14:24:59.26 Intake.select :open @14:25:01.42 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:25:01.44 SS.reconfigure :inhaling 0.38ml/s @14:25:01.49 SS.seek 25ml @14:26:02.57 Vacuum too high -- waiting up to 40 seconds @14:26:02.59 SS.reconfigure :exhale @14:26:02.64 SS.hold @14:26:12.59 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:26:12.61 SS.reconfigure :inhaling 0.33ml/s @14:26:12.66 SS.seek 25ml @14:26:21.55 Stroke completed -- waiting for pressure to equalize ... @14:26:21.56 SS.reconfigure :exhale @14:26:21.61 SS.hold @14:26:27.60 recoverPSRreagent08 @14:26:27.61 PTV.dial :puck @14:26:27.62 PBV.dial :waste1 @14:26:28.01 delay 30 seconds @14:26:42.56 Intake.select :close @14:26:42.74 SSV.dial :exhaust @14:26:43.51 Exhaust.select :open @14:26:43.63 SS.seek 0ml @14:26:53.18 Exhaust.select :close @14:26:53.29 SSV.dial CBV @14:26:53.33 CBV.dial SSV @14:26:54.10 Allowing 130s for 25.0ml of remaining 350.0ml @14:26:54.11 Intake.select :open @14:26:56.28 Intake @16.0psia, vacuum from 10.0 to 13.0psi @14:26:56.30 SS.reconfigure :inhaling 0.33ml/s @14:26:56.35 SS.seek 25ml @14:26:58.03 PBV.dial between PSV and waste1 @14:26:58.42 PSR.select :air @14:26:58.44 PSV.dial PSR @14:26:59.21 PS.reconfigure :fast @14:26:59.26 PS.seek 2.5ml @14:27:22.92 PSV.dial PTV @14:27:23.70 delay 5 seconds @14:27:28.72 PSV.dial PSR @14:27:29.50 delay 5 seconds @14:27:34.52 PSV.dial PTV @14:27:35.28 delay 5 seconds @14:27:40.30 PSV.dial :air @14:27:41.06 PS.seek 0ml @14:28:00.36 Vacuum too high -- waiting up to 40 seconds @14:28:00.37 SS.reconfigure :exhale @14:28:00.43 SS.hold @14:28:04.48 washCycleDA #3 of 3 @14:28:04.49 PSR.select :air @14:28:04.51 PSV.dial PSR @14:28:05.84 Pulling 0.5ml of PSR.wash @14:28:05.85 PSR.select :wash @14:28:06.53 PS.reconfigure :default @14:28:06.58 PS.seek 0.5ml @14:28:08.42 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:28:08.44 SS.reconfigure :inhaling 0.30ml/s @14:28:08.49 SS.seek 25ml @14:28:12.48 delay 5 seconds @14:28:17.50 PSR.select :air @14:28:17.62 PS.seek 2ml @14:28:25.16 Stroke completed -- waiting for pressure to equalize ... @14:28:25.17 SS.reconfigure :exhale @14:28:25.22 SS.hold @14:28:33.79 PTV.dial :puck @14:28:33.83 PSV.dial PTV @14:28:34.59 PBV.dial :waste1 @14:28:35.09 PS.seek 1.5ml @14:28:41.20 delay 3 seconds @14:28:44.21 PBV.dial between PSV and waste1 @14:28:44.61 PS.seek 0ml @14:28:47.68 Intake.select :close @14:28:47.84 SSV.dial :exhaust @14:28:48.60 Exhaust.select :open @14:28:48.71 SS.seek 0ml @14:28:58.24 Exhaust.select :close @14:28:58.35 SSV.dial CBV @14:28:58.39 CBV.dial SSV @14:28:59.16 Allowing 130s for 25.0ml of remaining 325.0ml @14:28:59.17 Intake.select :open @14:29:00.70 delay 2:00 @14:29:01.33 Intake @16.2psia, vacuum from 10.0 to 13.0psi @14:29:01.35 SS.reconfigure :inhaling 0.30ml/s @14:29:01.40 SS.seek 25ml @14:30:15.29 Vacuum too high -- waiting up to 40 seconds @14:30:15.31 SS.reconfigure :exhale @14:30:15.36 SS.hold @14:30:23.95 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:30:23.97 SS.reconfigure :inhaling 0.28ml/s @14:30:24.02 SS.seek 25ml @14:30:38.15 Stroke completed -- waiting for pressure to equalize ... @14:30:38.17 SS.reconfigure :exhale @14:30:38.22 SS.hold @14:31:00.72 recoverPSRreagent08 @14:31:00.73 PTV.dial :puck @14:31:00.74 PBV.dial :waste1 @14:31:01.13 delay 30 seconds @14:31:09.19 SS.reconfigure :default @14:31:09.24 SS.hold @14:31:09.30 Intake.select :close @14:31:09.46 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 700.0ml @14:31:09.48 SSV.dial :exhaust @14:31:10.25 Exhaust.select :open @14:31:10.36 SS.reconfigure :exhale @14:31:10.41 SS.seek 0ml @14:31:19.94 Exhaust.select :close @14:31:20.05 Sampled 700.0ml @14:31:20.07 Drying puck before fixing for 4:00 @14:31:20.08 CBV.dial :waste2b @14:31:20.84 delay 1:00 @14:31:31.15 PBV.dial between PSV and waste1 @14:31:31.54 PSR.select :air @14:31:31.56 PSV.dial PSR @14:31:32.33 PS.reconfigure :fast @14:31:32.38 PS.seek 2.5ml @14:31:56.32 PSV.dial PTV @14:31:57.07 delay 5 seconds @14:32:02.09 PSV.dial PSR @14:32:02.84 delay 5 seconds @14:32:07.86 PSV.dial PTV @14:32:08.60 delay 5 seconds @14:32:13.62 PSV.dial :air @14:32:14.40 PS.seek 0ml @14:32:20.86 CSV.dial :air @14:32:21.44 CS.reconfigure :fast @14:32:21.49 CS.seek 10ml @14:32:37.76 Gate.power :camera,:ON @14:32:37.77 Mixing DA Substrates @14:32:37.78 PSR.select :air @14:32:37.80 PSV.dial PSR @14:32:39.27 Pulling 0.25ml of PSR.sub1 @14:32:39.30 PSR.select :sub1 @14:32:39.50 PS.reconfigure :default @14:32:39.61 PS.seek 0.25ml @14:32:43.04 delay 5 seconds @14:32:45.45 Pumping wcr puck from tube 3 up to 35psi... @14:32:45.47 CBV.dial SSV @14:32:46.75 CBV.dial between CSV and waste2b @14:32:48.06 Pulling 0.25ml of PSR.sub2 @14:32:48.07 PSR.select :sub2 @14:32:48.24 CSV.dial between air and CTV @14:32:48.31 PS.seek 0.5ml @14:32:48.55 CS.seek 6.653ml @14:32:51.73 delay 5 seconds @14:32:56.75 PSR.select :air @14:32:56.86 PS.seek 2ml @14:32:57.17 CTV.dial CSV,avoiding: :air @14:32:57.20 CSV.dial CTV @14:32:58.03 CS.reconfigure :pressurize @14:32:58.09 CS.seek 0ml @14:33:12.98 PTV.dial :puck @14:33:12.99 PSV.dial PTV @14:33:13.76 PBV.dial :waste1 @14:33:13.91 CBV.dial :waste2b @14:33:14.21 PS.seek 1.5ml @14:33:14.28 CS.reconfigure :default @14:33:14.34 CS.seek 0ml @14:33:16.82 delay 1:00 @14:33:20.13 delay 3 seconds @14:33:23.15 PBV.dial between PSV and waste1 @14:33:23.54 PS.seek 0ml @14:33:39.63 delay 10 seconds @14:33:49.66 recoverPSRreagent08 @14:33:49.67 PTV.dial :puck @14:33:49.68 PBV.dial :waste1 @14:33:50.07 delay 30 seconds @14:34:16.85 CTV.dial :air,avoiding: :intake @14:34:18.24 Fix #1 of 1 (with RNAlater) for 4:00 @14:34:18.25 CSV.dial CSR @14:34:18.68 Pulling 2ml of CSR.RNAlater @14:34:18.69 CSR.select :RNAlater @14:34:18.81 CS.seek 2ml @14:34:20.09 PBV.dial between PSV and waste1 @14:34:20.47 PSR.select :air @14:34:20.49 PSV.dial PSR @14:34:21.25 PS.reconfigure :fast @14:34:21.30 PS.seek 2.5ml @14:34:24.82 delay 5 seconds @14:34:29.85 CSR.select :air @14:34:29.96 CS.reconfigure :fast @14:34:30.01 CS.seek 10ml @14:34:44.76 PSV.dial PTV @14:34:45.51 delay 5 seconds @14:34:49.33 Pumping wcr puck from tube 3 up to 35psi... @14:34:49.34 CBV.dial SSV @14:34:50.52 PSV.dial PSR @14:34:50.59 CBV.dial between CSV and waste2b @14:34:51.27 delay 5 seconds @14:34:52.07 CSV.dial between air and CTV @14:34:52.68 CS.seek 6.553ml @14:34:56.29 PSV.dial PTV @14:34:57.04 delay 5 seconds @14:35:01.40 CTV.dial CSV,avoiding: :air @14:35:01.43 CSV.dial CTV @14:35:02.06 PSV.dial :air @14:35:02.85 PS.seek 0ml @14:35:02.92 CS.reconfigure :pressurize @14:35:02.97 CS.seek 0ml @14:35:16.88 delay 4:00 @14:35:26.19 Additional air clear @14:35:26.20 PSR.select :air @14:35:26.22 PSV.dial PSR @14:35:27.55 Pulling 2ml of PSR.air @14:35:27.56 PSR.select :air @14:35:27.58 PS.reconfigure :default @14:35:27.63 PS.seek 2ml @14:35:48.82 delay 5 seconds @14:35:53.84 PTV.dial :puck @14:35:53.85 PSV.dial PTV @14:35:54.60 PBV.dial :waste1 @14:35:55.05 PS.seek 1.5ml @14:36:01.07 delay 3 seconds @14:36:04.09 PBV.dial between PSV and waste1 @14:36:04.47 delay 1 second @14:36:05.49 PSV.dial :waste1 @14:36:06.55 PS.seek 0ml @14:36:22.71 Processing Puck Heater at 27.9C before imaging @14:36:22.72 PH.coast @14:36:22.84 Imaging da puck in Processing Clamp while storing it to tube 2 @14:36:23.02 SC.reconfigure :tight @14:36:23.07 SC.seek 2 @14:36:26.69 SC.configure :limp @14:36:26.90 Elbow.reconfigure :tight @14:36:26.95 Elbow.seek 2 @14:36:28.66 Forearm.reconfigure :tight @14:36:28.71 Forearm.seek 2 + 800 counts @14:36:29.71 SE.reconfigure :pick @14:36:29.76 SE.seek :beamBreak @14:36:32.13 SE.configure :default @14:36:32.80 Elbow.reconfigure :limp @14:36:32.85 Forearm.reconfigure :loose @14:36:32.90 Forearm.seek 2 @14:36:33.30 Elbow.hold @14:36:33.37 Elbow.reconfigure :tight @14:36:33.47 SE.seek 7.09 Pucks below Top Plate @14:36:34.00 Forearm.force -20 @14:36:34.05 Forearm.seek 2 + 310 counts @14:36:34.39 Forearm.reconfigure :tight @14:36:34.44 Forearm.seek 2 + 800 counts @14:36:34.73 SE.seek 8.09 Pucks below Top Plate @14:36:35.31 Forearm.reconfigure :loose @14:36:35.36 Forearm.seek :retract @14:36:36.26 Elbow.seek PC @14:36:38.78 Elbow.reconfigure :loose @14:36:38.88 Forearm.seek PC @14:36:39.97 Hand.close @14:36:40.74 PC.seek :open @14:36:45.30 Forearm.seek :retract @14:36:46.38 Elbow.reconfigure :tight @14:36:46.43 Elbow.seek 2 @14:36:49.06 Forearm.reconfigure :tight @14:36:49.11 Forearm.seek 2 + 800 counts @14:36:50.10 Hand.open @14:36:50.93 SE.seek 7.85 Pucks below Top Plate @14:36:51.22 SE.reconfigure :seal @14:36:51.27 SE.seek 7.19 Pucks below Top Plate @14:36:51.80 Lights, camera, ACTION! @14:36:51.83 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da25oct1614h646ml.tif @14:37:11.32 Waiting >20 seconds for da to relinquish FlushPuck @14:37:12.28 SX HX9/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 17.15 seconds /var/log/esp/da25oct1614h646ml.tif: TIFF Upload Complete @14:37:12.31 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da25oct1614h646ml15s.tif @14:37:27.92 SX HX9/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da25oct1614h646ml15s.tif: TIFF Upload Complete @14:37:27.95 PuckCamera: snap -bin=2,2 21.0 /var/log/esp/midres/da25oct1614h646ml21s.tif @14:37:49.56 SX HX9/16 (USB:07): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21 seconds /var/log/esp/midres/da25oct1614h646ml21s.tif: TIFF Upload Complete @14:37:49.58 Gate.power :camera,:OFF @14:37:49.60 DAfans.email "17.15 sec auto-exposure",Subject: "da25oct1614h646ml images!" @14:37:49.89 Elbow.seek 2 @14:37:50.82 Forearm.seek 2 + 800 counts @14:37:50.92 SE.reconfigure :gently @14:37:50.98 SE.seek 7.91 Pucks below Top Plate @14:37:51.74 Elbow.reconfigure :limp @14:37:51.81 Forearm.reconfigure :loose @14:37:51.85 Forearm.seek 2 @14:37:52.24 Elbow.hold @14:37:52.33 Elbow.reconfigure :tight @14:37:52.41 SE.reconfigure :default @14:37:52.46 SE.seek 8.09 Pucks below Top Plate @14:37:52.86 Forearm.force -20 @14:37:52.90 Forearm.seek 2 + 307 counts @14:37:53.51 Forearm.reconfigure :tight @14:37:53.62 Forearm.seek 2 + 800 counts @14:37:53.95 SE.seek :down @14:37:55.22 Forearm.reconfigure :loose @14:37:55.32 Forearm.seek :retract @14:37:55.94 SE.stop @14:37:56.51 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:37:56.91 Elbow.seek FlushPuck::Garage @14:37:58.84 Elbow.reconfigure :loose @14:37:59.05 Forearm.seek :garage @14:38:00.10 Hand.close @14:38:01.13 Forearm.seek :retract @14:38:02.30 Elbow.reconfigure :tight @14:38:02.40 Elbow.seek PC @14:38:03.81 Elbow.reconfigure :loose @14:38:05.10 Forearm.reconfigure :tight @14:38:05.21 Forearm.seek PC @14:38:06.88 PC.seek :closed @14:38:11.48 Processing Clamp onSpring/offSpring for 12/26 samples, on/off torque 1.54 @14:38:11.61 Hand.open @14:38:12.50 Forearm.reconfigure :loose @14:38:12.60 Forearm.seek :retract @14:38:13.69 PSR.select :air @14:38:13.73 PSV.dial PSR @14:38:14.24 Pulling 0.5ml of PSR.flush @14:38:14.26 PSR.select :flush @14:38:14.43 PS.seek 0.5ml @14:38:20.40 delay 5 seconds @14:38:24.49 upsync @14:38:25.43 PSR.select :air @14:38:25.56 PS.seek 2.5ml @14:38:46.88 delay 1 second @14:38:47.90 PRV.dial :waste2,avoiding: :ab2 @14:38:49.77 PTV.dial :PRVmixing @14:38:51.10 PSV.dial :mixloop @14:38:52.17 PS.seek :empty @14:39:16.90 CBV.dial :waste2b @14:39:17.25 CS.reconfigure :default @14:39:17.30 CS.seek 0ml @14:39:18.63 PSR.select :air @14:39:18.65 PSV.dial PSR @14:39:19.72 Pulling 0.5ml of PSR.flush @14:39:19.73 PSR.select :flush @14:39:19.85 PS.seek 0.5ml @14:39:21.39 delay 1:00 @14:39:25.81 delay 5 seconds @14:39:30.83 PSR.select :air @14:39:30.95 PS.seek 2.5ml @14:39:52.25 delay 1 second @14:39:53.27 PBV.dial :waste1 @14:39:53.66 PTV.dial :mixing @14:39:54.41 PSV.dial :mixloop @14:39:55.51 PS.seek :empty @14:40:21.41 CTV.dial :air,avoiding: :intake @14:40:21.88 PSV.dial PSR @14:40:22.90 Storing wcr puck in Collection Clamp to tube 2 @14:40:22.99 PSR.select :flush @14:40:23.29 PS.seek 1ml @14:40:23.48 Elbow.reconfigure :tight @14:40:23.53 Elbow.seek 2 @14:40:26.11 Forearm.reconfigure :tight @14:40:26.16 Forearm.seek 2 + 800 counts @14:40:27.25 SE.reconfigure :pick @14:40:27.31 SE.seek :beamBreak @14:40:29.63 SE.configure :default @14:40:29.79 Elbow.reconfigure :limp @14:40:29.85 Forearm.reconfigure :loose @14:40:29.90 Forearm.seek 2 @14:40:30.30 Elbow.hold @14:40:30.37 Elbow.reconfigure :tight @14:40:30.47 SE.seek 8.08 Pucks below Top Plate @14:40:31.03 Forearm.force -20 @14:40:31.07 Forearm.seek 2 + 307 counts @14:40:31.37 Forearm.reconfigure :tight @14:40:31.42 Forearm.seek 2 + 800 counts @14:40:31.74 SE.seek 9.08 Pucks below Top Plate @14:40:32.43 Forearm.reconfigure :loose @14:40:32.48 Forearm.seek :retract @14:40:33.44 Elbow.seek CC @14:40:34.37 PSR.select :air @14:40:35.01 Elbow.reconfigure :loose @14:40:35.08 PS.seek 2.5ml @14:40:35.20 Forearm.seek CC @14:40:36.23 Hand.close @14:40:36.99 CC.seek :open @14:40:41.21 Forearm.seek :retract @14:40:42.27 Elbow.reconfigure :tight @14:40:42.32 Elbow.seek 2 @14:40:44.09 Elbow.reconfigure :limp @14:40:44.14 Forearm.seek 2 @14:40:45.27 Elbow.hold @14:40:45.34 Elbow.reconfigure :tight @14:40:45.39 Hand.open @14:40:46.24 Forearm.force -20 @14:40:46.29 Forearm.seek 2 + 319 counts @14:40:46.67 Forearm.reconfigure :tight @14:40:46.72 Forearm.seek 2 + 800 counts @14:40:47.04 SE.seek :down @14:40:48.18 Forearm.reconfigure :loose @14:40:48.23 Forearm.seek :retract @14:40:48.90 SE.stop @14:40:49.30
Starting finalPurge @14:40:51.23 PSV.dial PTV @14:40:51.99 PTV.dial :puck @14:40:52.74 PBV.dial :waste1 @14:40:52.82 PS.seek 0ml @14:41:08.95
Waiting >20 seconds for psFlush to relinquish FlushPuck @14:41:19.59 PSV.dial PSR @14:41:20.34 PSR.select :flush @14:41:20.47 PS.seek 0.5ml @14:41:26.40 PSR.select :air @14:41:26.52 PS.seek 2.5ml @14:41:47.84 PSV.dial PTV @14:41:48.61 PBV.dial PSV @14:41:49.19 PS.seek 0ml @14:42:15.65 PSV.dial PBV @14:42:16.24 PS.seek 2.5ml @14:42:42.59 PSV.dial :waste1 @14:42:43.35 PS.seek 0ml @14:43:09.65 PSV.dial PSR @14:43:10.11 PSR.select :air @14:43:10.13 PS.seek 2.5ml @14:43:36.50 PSV.dial PTV @14:43:37.25 PBV.dial PSV @14:43:37.33 PS.seek 0ml @14:44:03.65 PSV.dial PBV @14:44:04.13 PS.seek 2.5ml @14:44:30.84 PSV.dial :waste1 @14:44:31.58 PS.seek 0ml @14:44:58.03 PSV.dial PSR @14:44:58.50 PSR.select :flush @14:44:58.62 PS.seek 1ml @14:45:09.65 PSR.select :air @14:45:09.77 PS.seek 2.5ml @14:45:25.93 PSV.dial PTV @14:45:26.67 PTV.dial :puck @14:45:26.75 PBV.dial :waste1 @14:45:27.35 PS.seek 0ml @14:45:53.71 PSV.dial PSR @14:45:54.47 PSR.select :flush @14:45:54.59 PS.seek 0.5ml @14:46:00.59 PSR.select :air @14:46:00.71 PS.seek 2.5ml @14:46:22.03 PSV.dial PTV @14:46:22.79 PBV.dial PSV @14:46:23.38 PS.seek 0ml @14:46:49.93 PSV.dial PBV @14:46:50.41 PS.seek 2.5ml @14:47:16.84 PSV.dial :waste1 @14:47:18.06 PS.seek 0ml @14:47:44.40 PSV.dial PSR @14:47:44.87 PSR.select :air @14:47:44.90 PS.seek 2.5ml @14:48:11.34 PSV.dial PTV @14:48:12.09 PBV.dial PSV @14:48:12.17 PS.seek 0ml @14:48:38.56 PSV.dial PBV @14:48:39.03 PS.seek 2.5ml @14:49:05.49 PSV.dial :waste1 @14:49:06.25 PS.seek 0ml @14:49:32.81
Loading FlushPuck into Collection Clamp from Processing Clamp @14:49:33.00 Elbow.seek PC @14:49:35.53 Elbow.reconfigure :loose @14:49:35.63 Forearm.seek PC @14:49:36.71 Hand.close @14:49:37.48 PC.seek :open @14:49:42.02 Forearm.seek :retract @14:49:43.07 Elbow.reconfigure :tight @14:49:43.12 Elbow.seek CC @14:49:46.72 Elbow.reconfigure :loose @14:49:46.82 Forearm.reconfigure :tight @14:49:46.86 Forearm.seek CC @14:49:48.11 CC.seek :closed @14:49:52.10 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.03 @14:49:52.18 Hand.open @14:49:53.05 Forearm.reconfigure :loose @14:49:53.09 Forearm.seek :retract @14:49:54.53 Flushing the Collection Valve::Manifold @14:49:54.66 CSV.dial CSR @14:49:55.20 Pulling 5ml of CSR.flush @14:49:55.21 CSR.select :flush @14:49:55.33 CS.seek 5ml @14:50:08.97 delay 5 seconds @14:50:13.99 CSR.select :air @14:50:14.16 CS.reconfigure :fast @14:50:14.20 CS.seek 7.5ml @14:50:20.81 CSV.dial :waste @14:50:22.08 CS.reconfigure :default @14:50:22.13 CS.seek 0ml @14:50:42.12 Flushing lower manifold's waste1 port @14:50:42.25 CSV.dial :flush @14:50:42.71 CS.reconfigure :fast @14:50:42.75 CS.seek 5ml @14:50:55.12 delay 5 seconds @14:51:00.14 CSV.dial :air @14:51:00.65 CS.seek 7ml @14:51:06.06 CSV.dial CTV @14:51:06.10
CTV.dial CSV @14:51:07.48 CBV.dial :waste1 @14:51:08.27 CS.seek 0ml @14:51:25.06 Flushing lower manifold's waste2 port @14:51:25.18 CSV.dial :flush @14:51:25.93 CS.seek 5ml @14:51:38.40 delay 5 seconds @14:51:43.42 CSV.dial :air @14:51:43.93 CS.seek 7ml @14:51:49.34 CSV.dial CTV @14:51:49.38
CTV.dial CSV @14:51:49.81 CBV.dial :waste2 @14:51:50.86 CS.seek 0ml @14:52:07.62 Flushing lower manifold's waste2b port @14:52:07.74 CSV.dial :flush @14:52:08.47 CS.seek 5ml @14:52:20.78 delay 5 seconds @14:52:25.80 CSV.dial :air @14:52:26.31 CS.seek 7ml @14:52:31.81 CSV.dial CTV @14:52:31.85
CTV.dial CSV @14:52:32.28 CBV.dial :waste2b @14:52:33.32 CS.seek 0ml @14:52:49.93 Disinfecting external Intake with kill @14:52:50.06 CSV.dial CSR @14:52:50.50 Pulling 8ml of CSR.kill @14:52:50.51 CSR.select :kill @14:52:51.09 CS.seek 8ml @14:53:10.21 delay 5 seconds @14:53:15.23 CSR.select :air @14:53:15.35 CS.seek 10ml @14:53:20.82 CSV.dial CBV @14:53:20.84
CBV.dial CSV @14:53:21.90 CTV.dial :intake @14:53:22.67 Intake.select :open @14:53:22.78 CS.seek 2ml @14:53:41.84 Intake.select :close @14:53:41.94 CTV.dial :air @14:53:42.72 CS.reconfigure :default @14:53:42.77 CS.seek 0ml @14:53:48.87 CSV.dial CSR @14:53:49.87 Pulling 5ml of CSR.flush @14:53:49.88 CSR.select :flush @14:53:50.00 CS.seek 5ml @14:54:03.56 delay 5 seconds @14:54:08.58 CSR.select :air @14:54:08.69 CS.seek 10ml @14:54:22.31 CSV.dial CBV @14:54:23.31 CS.seek 0ml @14:54:49.71 Garaging FlushPuck in Collection Clamp @14:54:49.78 Elbow.reconfigure :tight @14:54:49.83 Elbow.seek CC @14:54:49.95 Elbow.reconfigure :loose @14:54:50.05 Forearm.seek CC @14:54:51.09 Hand.close @14:54:51.85 CC.seek :open @14:54:55.98 Forearm.seek :retract @14:54:56.94 Elbow.reconfigure :tight @14:54:56.99 Elbow.seek :garage @14:54:59.83 Elbow.reconfigure :loose @14:54:59.93 Forearm.reconfigure :tight @14:54:59.98 Forearm.seek :garage @14:55:00.99 Hand.open @14:55:01.78 Forearm.reconfigure :loose @14:55:01.83 Forearm.seek :retract @14:55:02.80 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 12:39:43 @14:55:02.85 Asleep after completing DA_WCR_101625 assays @14:55:02.87 GoodNews.email "{1 => 22,\n 2 => 10,\n 3 => 12,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@14:49:09, 40% flow, 19.9C, 20% humidity, 14.6psia, 13.78V, 0.284A avg, 152.615Ah, 3.91W\nSampled 700.0ml",Subject: "Asleep after completing DA_WCR_101625 assays" @14:55:07.13 upsync + ESPwhich DA_WCR_102225.rb + fn=DA_WCR_102225.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_102225.rb + [ -f /home/esp/esp2/mission/DA_WCR_102225.rb ] + realpath /home/esp/esp2/mission/DA_WCR_102225.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_102225.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_102225.rb @16:26:32.53UTC22-Oct-25
ESP2 v3.42 I2C Gateway v3.20 for core bus @16:26:35.08 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @16:26:38.95 Polling Can every 10:00 @16:26:38.99 Cleanly exited at 16:22:27UTC22-Oct-25 @16:26:39.04 Loading /home/esp/esp2local/mission/DA_WCR_102225.rb mission @16:26:39.09 Preparing to sleep until + 00:00:05 @16:26:39.13 GoodNews.email "Starting DA_WCR_102225 w/pucks in tube 3\n{1 => 22,\n 2 => 10,\n 3 => 12,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@16:26:38, 40% flow, 20.2C, 22% humidity, 14.6psia, 13.73V, 0.277A avg, 180.378Ah, 3.81W",Subject: "DA_WCR_102225 begun" @16:26:39.16
Asleep til + 00:00:05 @16:26:39.19 Continuing at 16:26 @16:26:39.43 Gate.power :core,:OFF @16:26:39.44 Gate.power :raw,:OFF @16:26:39.45 Shutdown @16:26:41.64 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_102225.rb:14\n{1 => 22,\n 2 => 10,\n 3 => 12,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@16:26:39, 40% flow, 20.2C, 22% humidity, 14.6psia, 13.73V, 0.277A avg, 180.378Ah, 3.81W",Subject: "Asleep until 16:26" @16:26:43.07 upsync @16:26:44.19
Powering core bus @16:26:45.55 upsync @16:26:49.41
Gate.power :raw,:ON @16:26:50.22 CS.configure :default @16:26:50.27 PS.configure :default @16:26:50.32 SS.configure :default @16:26:50.37 CC.configure :default @16:26:50.42 PC.configure :default @16:26:50.48 Forearm.configure :loose @16:26:50.53 Elbow.configure :loose @16:26:50.60 SC.configure :loose @16:26:50.65 SE.configure :default @16:26:50.70 CH.configure :default @16:26:50.73 PH.configure :default @16:26:50.77 All dwarf controllers running firmware version 3.85 @16:26:50.88 Valve.reset @16:26:51.52 Intake.select :close @16:26:51.56 Exhaust.select :close @16:26:51.57 CTV.dial :air @16:26:51.65 PSV.dial :air @16:26:51.71 CSV.dial :waste1 @16:26:51.84
Forearm.home.seek :retract @16:26:52.20 SSV.dial :spit,avoiding: :exhaust @16:26:52.62 Hand.close @16:26:52.65
Elbow.reconfigure :loose @16:26:52.75 Elbow.jog 1500 @16:26:52.82 PS.home.seek 0ml @16:26:53.09 CS.reconfigure :fast @16:26:53.13 SS.home.seek 0ml @16:26:53.23 CS.home.seek 0ml @16:26:53.29
Elbow.reconfigure :loose @16:26:53.34 Elbow.home.seek :clear @16:26:56.57 SE.home.seek :down @16:26:57.24 SC.reconfigure :tight @16:26:57.29 SC.home.seek :initial @16:27:01.95 PS.stop @16:27:09.95
SC.configure :limp @16:27:10.01 Forearm.seek :retract @16:27:10.08 Elbow.seek :hammer @16:27:11.11 Forearm.reconfigure :tight @16:27:11.16 Forearm.seek retracted @16:27:11.40 PC.home.seek :open @16:27:11.42
CC.home.seek :open @16:27:14.62 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @16:27:14.63 Notice.email "At 16:27:14 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_102225.rb:14\n{1 => 22,\n 2 => 10,\n 3 => 12,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@16:27:14, 40% flow, 20.3C, 22% humidity, 14.6psia, 13.66V, 0.290A avg, 180.382Ah, 3.96W",Subject: "Started DA_WCR_102225 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @16:27:14.65
Starting initialPurge @16:27:14.68 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:27:14.74 Hand.open @16:27:15.63 Elbow.reconfigure :tight @16:27:15.68 Elbow.seek FlushPuck::Garage @16:27:17.67 Elbow.reconfigure :loose @16:27:17.90 Forearm.reconfigure :loose @16:27:17.95 Forearm.seek :garage @16:27:18.98 Hand.close @16:27:19.04 upsync @16:27:19.87
Forearm.seek :retract @16:27:20.87 Elbow.reconfigure :tight @16:27:20.92 Elbow.seek CC @16:27:23.73 Elbow.reconfigure :loose @16:27:23.83 Forearm.reconfigure :tight @16:27:23.87 Forearm.seek CC @16:27:25.19 CC.seek :closed @16:27:29.21 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.02 @16:27:29.28 Hand.open @16:27:30.06 Forearm.reconfigure :loose @16:27:30.11 Forearm.seek :retract @16:27:31.17 Flushing the Collection Valve::Manifold @16:27:31.29 CSV.dial CSR @16:27:32.57 Pulling 5ml of CSR.flush @16:27:32.58 CSR.select :flush @16:27:32.70 CS.reconfigure :default @16:27:32.75 CS.seek 5ml @16:27:46.84 delay 5 seconds @16:27:51.86 CSR.select :air @16:27:52.03 CS.reconfigure :fast @16:27:52.07 CS.seek 7.5ml @16:27:58.71 CSV.dial :waste @16:28:00.00 CS.reconfigure :default @16:28:00.05 CS.seek 0ml @16:28:20.03 Purging external Intake @16:28:20.15 CSV.dial :flush @16:28:20.59 CS.reconfigure :fast @16:28:20.64 CS.seek 10ml @16:28:44.71 delay 5 seconds @16:28:49.73 CSV.dial CBV @16:28:49.77
CBV.dial CSV @16:28:50.51 CTV.dial :intake @16:28:51.29 Intake.select :open @16:28:51.41 CS.seek 0ml @16:29:15.12 Intake.select :close @16:29:15.22 CTV.dial :air @16:29:16.00 Flushing upper manifold @16:29:16.12 CSV.dial :flush @16:29:16.97 CS.seek 5ml @16:29:29.31 delay 5 seconds @16:29:34.33 CSV.dial :air @16:29:34.98 CS.seek 7ml @16:29:40.46 CSV.dial CBV @16:29:40.49
CBV.dial CSV @16:29:41.51 CTV.dial :air @16:29:41.59 CS.seek 0ml @16:29:58.52 delay 7 seconds @16:30:05.55 CS.seek 3ml @16:30:13.33 CSV.dial :waste @16:30:13.80 CS.seek 0ml @16:30:21.69 CSV.dial :flush @16:30:22.14 CS.seek 3ml @16:30:29.93 delay 5 seconds @16:30:34.95 CSV.dial :air @16:30:35.48 CS.seek 4.999ml @16:30:41.18 CSV.dial :air @16:30:41.27 CS.seek 0ml @16:30:53.58 delay 7 seconds @16:31:00.61 CS.seek 3ml @16:31:08.43 CSV.dial :waste @16:31:09.17 CS.seek 0ml @16:31:16.93 Garaging FlushPuck in Collection Clamp @16:31:17.01 Elbow.reconfigure :tight @16:31:17.05 Elbow.seek CC @16:31:17.17 Elbow.reconfigure :loose @16:31:17.28 Forearm.seek CC @16:31:18.26 Hand.close @16:31:19.00 CC.seek :open @16:31:23.49 Forearm.seek :retract @16:31:24.46 Elbow.reconfigure :tight @16:31:24.51 Elbow.seek :garage @16:31:27.37 Elbow.reconfigure :loose @16:31:27.47 Forearm.reconfigure :tight @16:31:27.52 Forearm.seek :garage @16:31:28.58 Hand.open @16:31:29.43 Forearm.reconfigure :loose @16:31:29.47 Forearm.seek :retract @16:31:30.45 da sampling at most 1000ml, wcr at most 1000ml @16:31:30.49 Loading sh1 puck from tube 3 into Collection Clamp @16:31:30.50 DAfans.email "Started da in tube 3 at 16:31:30UTC22-Oct-25\n{1 => 22,\n 2 => 10,\n 3 => 12,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@16:27:14, 40% flow, 20.3C, 22% humidity, 14.6psia, 13.66V, 0.290A avg, 180.382Ah, 3.96W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @16:31:30.79
SC.reconfigure :tight @16:31:30.84 SC.seek 3 @16:31:34.43 upsync @16:31:34.55
SC.configure :limp @16:31:34.78 Elbow.reconfigure :tight @16:31:34.83 Elbow.seek 3 @16:31:36.76 Forearm.reconfigure :tight @16:31:36.81 Forearm.seek 3 + 800 counts @16:31:37.84 SE.reconfigure :pick @16:31:37.89 SE.seek :beamBreak @16:31:39.90 SE.configure :default @16:31:40.07 Elbow.seek 3 @16:31:40.26 Forearm.seek 3 + 300 counts @16:31:40.43 Forearm.reconfigure :loose @16:31:40.48 Hand.close @16:31:41.25 SE.seek :down @16:31:41.89
Forearm.seek :retract @16:31:42.84 Elbow.seek CC @16:31:43.01 SE.stop @16:31:44.41
Elbow.reconfigure :loose @16:31:44.51 Forearm.reconfigure :tight @16:31:44.56 Forearm.seek CC @16:31:45.79 CC.seek :closed @16:31:49.78 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.07 @16:31:49.80 Hand.open @16:31:50.56 Forearm.reconfigure :loose @16:31:50.60 Forearm.seek :retract @16:31:51.59 collectSample 1000ml w/bubble point of 24psi @16:31:51.60 Priming 7ml @16:31:51.61 Intake.select :close @16:31:51.62 Exhaust.select :close @16:31:51.63 SS.hold @16:31:51.84 Calibrating pressure and temperature sensors @16:31:51.87 CTV.dial :air @16:31:51.89 SSV.dial :spit,avoiding: :exhaust @16:31:51.97 SS.reconfigure :fast @16:31:52.02 SS.seek 5ml @16:31:54.98 SSV.dial CBV @16:31:55.01
CBV.dial SSV @16:31:56.39 delay 3.8 seconds @16:32:00.32 Sensor limits: Intake max 99.0psia; Outlet max 111.1psia @16:32:00.33 SSV.dial :spit @16:32:01.68 SS.seek 0ml @16:32:04.22 SSV.dial CBV @16:32:05.55 CTV.dial :intake @16:32:06.32 SSV.dial CBV @16:32:06.35
CBV.dial SSV @16:32:06.44 Allowing 40s for 7.0ml of remaining 7.0ml @16:32:06.45 Intake.select :open @16:32:08.62 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:32:08.63 SS.reconfigure :inhaling 2.17ml/s @16:32:08.68 SS.seek 7ml @16:32:15.14 Stroke completed -- waiting for pressure to equalize ... @16:32:15.15 SS.reconfigure :exhale @16:32:15.19 SS.hold @16:32:15.45 Intake.select :close @16:32:15.60 SSV.dial :exhaust @16:32:16.36 Exhaust.select :open @16:32:16.48 SS.seek 0ml @16:32:20.40 Exhaust.select :close @16:32:20.93 Sampling 1000.0ml @16:32:20.94 Intake.select :close @16:32:20.96 Exhaust.select :close @16:32:20.97 SS.reconfigure :default @16:32:21.01 SS.hold @16:32:21.23 CTV.dial :intake @16:32:21.40 SSV.dial CBV @16:32:21.43
CBV.dial SSV @16:32:22.19 Allowing 130s for 25.0ml of remaining 1000.0ml @16:32:22.20 Intake.select :open @16:32:24.36 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:32:24.38 SS.reconfigure :inhaling 2.17ml/s @16:32:24.43 SS.seek 25ml @16:32:39.53 Stroke completed -- waiting for pressure to equalize ... @16:32:39.54 SS.reconfigure :exhale @16:32:39.58 SS.hold @16:32:45.19 Intake.select :close @16:32:45.35 SSV.dial :exhaust @16:32:46.12 Exhaust.select :open @16:32:46.24 SS.seek 0ml @16:32:55.56 Exhaust.select :close @16:32:55.67 SSV.dial CBV @16:32:55.69
CBV.dial SSV @16:32:56.47 Allowing 130s for 25.0ml of remaining 975.0ml @16:32:56.48 Intake.select :open @16:32:58.64 Intake @13.8psia, vacuum from 8.3 to 11.3psi @16:32:58.66 SS.reconfigure :inhaling 2.17ml/s @16:32:58.71 SS.seek 25ml @16:33:12.68 Stroke completed -- waiting for pressure to equalize ... @16:33:12.69 SS.reconfigure :exhale @16:33:12.74 SS.hold @16:33:19.29 Intake.select :close @16:33:19.45 SSV.dial :exhaust @16:33:20.22 Exhaust.select :open @16:33:20.33 SS.seek 0ml @16:33:29.61 Exhaust.select :close @16:33:29.72 SSV.dial CBV @16:33:29.74
CBV.dial SSV @16:33:30.51 Allowing 130s for 25.0ml of remaining 950.0ml @16:33:30.52 Intake.select :open @16:33:32.69 Intake @13.8psia, vacuum from 8.3 to 11.3psi @16:33:32.71 SS.reconfigure :inhaling 2.17ml/s @16:33:32.76 SS.seek 25ml @16:33:46.90 Stroke completed -- waiting for pressure to equalize ... @16:33:46.91 SS.reconfigure :exhale @16:33:46.96 SS.hold @16:33:53.44 Intake.select :close @16:33:53.59 SSV.dial :exhaust @16:33:54.37 Exhaust.select :open @16:33:54.49 SS.seek 0ml @16:34:03.85 Exhaust.select :close @16:34:03.96 SSV.dial CBV @16:34:03.98
CBV.dial SSV @16:34:04.76 Allowing 130s for 25.0ml of remaining 925.0ml @16:34:04.77 Intake.select :open @16:34:06.93 Intake @13.7psia, vacuum from 8.2 to 11.2psi @16:34:06.95 SS.reconfigure :inhaling 2.17ml/s @16:34:07.00 SS.seek 25ml @16:34:21.32 Stroke completed -- waiting for pressure to equalize ... @16:34:21.33 SS.reconfigure :exhale @16:34:21.38 SS.hold @16:34:28.13 Intake.select :close @16:34:28.29 SSV.dial :exhaust @16:34:29.06 Exhaust.select :open @16:34:29.17 SS.seek 0ml @16:34:38.46 Exhaust.select :close @16:34:38.57 SSV.dial CBV @16:34:38.59
CBV.dial SSV @16:34:39.39 Allowing 130s for 25.0ml of remaining 900.0ml @16:34:39.40 Intake.select :open @16:34:41.56 Intake @14.0psia, vacuum from 8.5 to 11.5psi @16:34:41.58 SS.reconfigure :inhaling 2.17ml/s @16:34:41.63 SS.seek 25ml @16:34:55.73 Stroke completed -- waiting for pressure to equalize ... @16:34:55.74 SS.reconfigure :exhale @16:34:55.78 SS.hold @16:35:04.23 Intake.select :close @16:35:04.38 SSV.dial :exhaust @16:35:05.17 Exhaust.select :open @16:35:05.28 SS.seek 0ml @16:35:14.20 Exhaust.select :close @16:35:14.31 SSV.dial CBV @16:35:14.33
CBV.dial SSV @16:35:15.12 Allowing 130s for 25.0ml of remaining 875.0ml @16:35:15.13 Intake.select :open @16:35:17.30 Intake @14.0psia, vacuum from 8.5 to 11.5psi @16:35:17.31 SS.reconfigure :inhaling 2.17ml/s @16:35:17.36 SS.seek 25ml @16:35:31.82 Stroke completed -- waiting for pressure to equalize ... @16:35:31.83 SS.reconfigure :exhale @16:35:31.88 SS.hold @16:35:40.41 Intake.select :close @16:35:40.57 SSV.dial :exhaust @16:35:41.34 Exhaust.select :open @16:35:41.45 SS.seek 0ml @16:35:50.81 Exhaust.select :close @16:35:50.91 SSV.dial CBV @16:35:50.94
CBV.dial SSV @16:35:51.73 Allowing 130s for 25.0ml of remaining 850.0ml @16:35:51.74 Intake.select :open @16:35:53.91 Intake @14.0psia, vacuum from 8.5 to 11.5psi @16:35:53.92 SS.reconfigure :inhaling 2.17ml/s @16:35:53.97 SS.seek 25ml @16:36:07.82 Stroke completed -- waiting for pressure to equalize ... @16:36:07.83 SS.reconfigure :exhale @16:36:07.88 SS.hold @16:36:16.87 Intake.select :close @16:36:17.03 SSV.dial :exhaust @16:36:17.81 Exhaust.select :open @16:36:17.92 SS.seek 0ml @16:36:27.10 Exhaust.select :close @16:36:27.21 SSV.dial CBV @16:36:27.25
CBV.dial SSV @16:36:28.01 Allowing 130s for 25.0ml of remaining 825.0ml @16:36:28.02 Intake.select :open @16:36:30.18 Intake @14.0psia, vacuum from 8.5 to 11.5psi @16:36:30.20 SS.reconfigure :inhaling 2.17ml/s @16:36:30.25 SS.seek 25ml @16:36:44.99 Stroke completed -- waiting for pressure to equalize ... @16:36:45.00 SS.reconfigure :exhale @16:36:45.05 SS.hold @16:36:55.25 Intake.select :close @16:36:55.41 SSV.dial :exhaust @16:36:56.18 Exhaust.select :open @16:36:56.29 SS.seek 0ml @16:37:05.49 Exhaust.select :close @16:37:05.60 SSV.dial CBV @16:37:05.64
CBV.dial SSV @16:37:06.38 Allowing 130s for 25.0ml of remaining 800.0ml @16:37:06.39 Intake.select :open @16:37:08.55 Intake @13.8psia, vacuum from 8.3 to 11.3psi @16:37:08.57 SS.reconfigure :inhaling 2.17ml/s @16:37:08.62 SS.seek 25ml @16:37:22.99 Stroke completed -- waiting for pressure to equalize ... @16:37:23.00 SS.reconfigure :exhale @16:37:23.05 SS.hold @16:37:34.17 Intake.select :close @16:37:34.32 SSV.dial :exhaust @16:37:35.09 Exhaust.select :open @16:37:35.20 SS.seek 0ml @16:37:44.41 Exhaust.select :close @16:37:44.52 SSV.dial CBV @16:37:44.57
CBV.dial SSV @16:37:45.30 Allowing 130s for 25.0ml of remaining 775.0ml @16:37:45.32 Intake.select :open @16:37:47.47 Intake @14.0psia, vacuum from 8.5 to 11.5psi @16:37:47.49 SS.reconfigure :inhaling 2.17ml/s @16:37:47.97 SS.seek 25ml @16:38:02.25 Stroke completed -- waiting for pressure to equalize ... @16:38:02.26 SS.reconfigure :exhale @16:38:02.31 SS.hold @16:38:14.87 Intake.select :close @16:38:15.02 SSV.dial :exhaust @16:38:15.80 Exhaust.select :open @16:38:15.91 SS.seek 0ml @16:38:25.13 Exhaust.select :close @16:38:25.24 SSV.dial CBV @16:38:25.27
CBV.dial SSV @16:38:26.05 Allowing 130s for 25.0ml of remaining 750.0ml @16:38:26.06 Intake.select :open @16:38:28.22 Intake @14.0psia, vacuum from 8.5 to 11.5psi @16:38:28.24 SS.reconfigure :inhaling 2.17ml/s @16:38:28.29 SS.seek 25ml @16:38:42.75 Stroke completed -- waiting for pressure to equalize ... @16:38:42.76 SS.reconfigure :exhale @16:38:42.81 SS.hold @16:38:57.26 Intake.select :close @16:38:57.41 SSV.dial :exhaust @16:38:58.19 Exhaust.select :open @16:38:58.30 SS.seek 0ml @16:39:07.60 Exhaust.select :close @16:39:07.71 SSV.dial CBV @16:39:07.74
CBV.dial SSV @16:39:08.52 Allowing 130s for 25.0ml of remaining 725.0ml @16:39:08.53 Intake.select :open @16:39:10.70 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:39:10.71 SS.reconfigure :inhaling 2.17ml/s @16:39:10.76 SS.seek 25ml @16:39:24.83 Stroke completed -- waiting for pressure to equalize ... @16:39:24.84 SS.reconfigure :exhale @16:39:24.89 SS.hold @16:39:39.68 Intake.select :close @16:39:39.83 SSV.dial :exhaust @16:39:40.61 Exhaust.select :open @16:39:40.73 SS.seek 0ml @16:39:50.00 Exhaust.select :close @16:39:50.11 SSV.dial CBV @16:39:50.13
CBV.dial SSV @16:39:50.91 Allowing 130s for 25.0ml of remaining 700.0ml @16:39:50.92 Intake.select :open @16:39:53.08 Intake @14.0psia, vacuum from 8.5 to 11.5psi @16:39:53.10 SS.reconfigure :inhaling 2.17ml/s @16:39:53.15 SS.seek 25ml @16:40:07.16 Stroke completed -- waiting for pressure to equalize ... @16:40:07.17 SS.reconfigure :exhale @16:40:07.21 SS.hold @16:40:23.88 Intake.select :close @16:40:24.45 SSV.dial :exhaust @16:40:25.24 Exhaust.select :open @16:40:25.35 SS.seek 0ml @16:40:34.21 Exhaust.select :close @16:40:34.32 SSV.dial CBV @16:40:34.34
CBV.dial SSV @16:40:35.13 Allowing 130s for 25.0ml of remaining 675.0ml @16:40:35.14 Intake.select :open @16:40:37.31 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:40:37.33 SS.reconfigure :inhaling 2.17ml/s @16:40:37.37 SS.seek 25ml @16:40:51.61 Stroke completed -- waiting for pressure to equalize ... @16:40:51.62 SS.reconfigure :exhale @16:40:51.67 SS.hold @16:41:09.98 Intake.select :close @16:41:10.13 SSV.dial :exhaust @16:41:10.91 Exhaust.select :open @16:41:11.02 SS.seek 0ml @16:41:20.24 Exhaust.select :close @16:41:20.35 SSV.dial CBV @16:41:20.37
CBV.dial SSV @16:41:21.16 Allowing 130s for 25.0ml of remaining 650.0ml @16:41:21.17 Intake.select :open @16:41:23.33 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:41:23.70 SS.reconfigure :inhaling 2.17ml/s @16:41:23.87 SS.seek 25ml @16:41:38.10 Stroke completed -- waiting for pressure to equalize ... @16:41:38.11 SS.reconfigure :exhale @16:41:38.15 SS.hold @16:41:58.45 Intake.select :close @16:41:58.61 SSV.dial :exhaust @16:41:59.40 Exhaust.select :open @16:41:59.51 SS.seek 0ml @16:42:08.74 Exhaust.select :close @16:42:08.85 SSV.dial CBV @16:42:08.87
CBV.dial SSV @16:42:09.64 Allowing 130s for 25.0ml of remaining 625.0ml @16:42:09.65 Intake.select :open @16:42:11.81 Intake @14.2psia, vacuum from 8.7 to 11.7psi @16:42:11.83 SS.reconfigure :inhaling 2.17ml/s @16:42:11.88 SS.seek 25ml @16:42:26.16 Stroke completed -- waiting for pressure to equalize ... @16:42:26.17 SS.reconfigure :exhale @16:42:26.21 SS.hold @16:42:48.79 Intake.select :close @16:42:48.95 SSV.dial :exhaust @16:42:49.72 Exhaust.select :open @16:42:49.84 SS.seek 0ml @16:42:59.02 Exhaust.select :close @16:42:59.13 SSV.dial CBV @16:42:59.15
CBV.dial SSV @16:42:59.92 Allowing 130s for 25.0ml of remaining 600.0ml @16:42:59.93 Intake.select :open @16:43:02.10 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:43:02.11 SS.reconfigure :inhaling 2.17ml/s @16:43:02.16 SS.seek 25ml @16:43:11.88 Vacuum too high -- waiting up to 40 seconds @16:43:11.89 SS.reconfigure :exhale @16:43:11.95 SS.hold @16:43:23.68 Intake @12.6psia, vacuum from 7.1 to 10.1psi @16:43:23.70 SS.reconfigure :inhaling 1.95ml/s @16:43:23.75 SS.seek 25ml @16:43:28.66 Vacuum too high -- waiting up to 40 seconds @16:43:28.68 SS.reconfigure :exhale @16:43:28.73 SS.hold @16:43:38.83 Intake @13.2psia, vacuum from 7.7 to 10.7psi @16:43:38.85 SS.reconfigure :inhaling 1.77ml/s @16:43:38.90 SS.seek 25ml @16:43:39.76 Stroke completed -- waiting for pressure to equalize ... @16:43:39.77 SS.reconfigure :exhale @16:43:39.82 SS.hold @16:43:42.56 Intake.select :close @16:43:42.72 SSV.dial :exhaust @16:43:43.48 Exhaust.select :open @16:43:43.59 SS.seek 0ml @16:43:52.45 Exhaust.select :close @16:43:52.56 SSV.dial CBV @16:43:52.59
CBV.dial SSV @16:43:53.36 Allowing 130s for 25.0ml of remaining 575.0ml @16:43:53.37 Intake.select :open @16:43:55.53 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:43:55.55 SS.reconfigure :inhaling 1.77ml/s @16:43:55.60 SS.seek 25ml @16:44:06.50 Vacuum too high -- waiting up to 40 seconds @16:44:06.52 SS.reconfigure :exhale @16:44:06.57 SS.hold @16:44:17.97 Intake @13.2psia, vacuum from 7.7 to 10.7psi @16:44:17.99 SS.reconfigure :inhaling 1.59ml/s @16:44:18.04 SS.seek 25ml @16:44:23.32 Vacuum too high -- waiting up to 40 seconds @16:44:23.69 SS.reconfigure :exhale @16:44:23.81 SS.hold @16:44:32.45 Intake @13.3psia, vacuum from 7.8 to 10.8psi @16:44:32.47 SS.reconfigure :inhaling 1.43ml/s @16:44:32.52 SS.seek 25ml @16:44:34.71 Stroke completed -- waiting for pressure to equalize ... @16:44:34.72 SS.reconfigure :exhale @16:44:34.77 SS.hold @16:44:41.17 Intake.select :close @16:44:41.32 SSV.dial :exhaust @16:44:42.52 Exhaust.select :open @16:44:42.63 SS.seek 0ml @16:44:51.53 Exhaust.select :close @16:44:51.64 SSV.dial CBV @16:44:51.67
CBV.dial SSV @16:44:52.44 Allowing 130s for 25.0ml of remaining 550.0ml @16:44:52.45 Intake.select :open @16:44:54.61 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:44:54.63 SS.reconfigure :inhaling 1.43ml/s @16:44:54.68 SS.seek 25ml @16:45:05.60 Vacuum too high -- waiting up to 40 seconds @16:45:05.61 SS.reconfigure :exhale @16:45:05.66 SS.hold @16:45:12.31 Intake @12.5psia, vacuum from 7.0 to 10.0psi @16:45:12.33 SS.reconfigure :inhaling 1.29ml/s @16:45:12.38 SS.seek 25ml @16:45:18.60 Vacuum too high -- waiting up to 40 seconds @16:45:18.61 SS.reconfigure :exhale @16:45:18.66 SS.hold @16:45:30.13 Intake @13.0psia, vacuum from 7.5 to 10.5psi @16:45:30.14 SS.reconfigure :inhaling 1.16ml/s @16:45:30.19 SS.seek 25ml @16:45:34.79 Stroke completed -- waiting for pressure to equalize ... @16:45:34.80 SS.reconfigure :exhale @16:45:34.85 SS.hold @16:45:44.60 Intake.select :close @16:45:44.75 SSV.dial :exhaust @16:45:45.52 Exhaust.select :open @16:45:45.63 SS.seek 0ml @16:45:55.07 Exhaust.select :close @16:45:55.18 SSV.dial CBV @16:45:55.21
CBV.dial SSV @16:45:56.00 Allowing 130s for 25.0ml of remaining 525.0ml @16:45:56.01 Intake.select :open @16:45:58.17 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:45:58.19 SS.reconfigure :inhaling 1.16ml/s @16:45:58.24 SS.seek 25ml @16:46:11.43 Vacuum too high -- waiting up to 40 seconds @16:46:11.44 SS.reconfigure :exhale @16:46:11.49 SS.hold @16:46:20.15 Intake @13.2psia, vacuum from 7.7 to 10.7psi @16:46:20.17 SS.reconfigure :inhaling 1.04ml/s @16:46:20.21 SS.seek 25ml @16:46:27.56 Vacuum too high -- waiting up to 40 seconds @16:46:27.58 SS.reconfigure :exhale @16:46:27.63 SS.hold @16:46:36.28 Intake @13.6psia, vacuum from 8.1 to 11.1psi @16:46:36.30 SS.reconfigure :inhaling 0.94ml/s @16:46:36.34 SS.seek 25ml @16:46:41.40 Stroke completed -- waiting for pressure to equalize ... @16:46:41.41 SS.reconfigure :exhale @16:46:41.46 SS.hold @16:46:52.59 Intake.select :close @16:46:52.74 SSV.dial :exhaust @16:46:53.51 Exhaust.select :open @16:46:53.62 SS.seek 0ml @16:47:03.01 Exhaust.select :close @16:47:03.12 SSV.dial CBV @16:47:03.16
CBV.dial SSV @16:47:03.90 Allowing 130s for 25.0ml of remaining 500.0ml @16:47:03.91 Intake.select :open @16:47:06.07 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:47:06.09 SS.reconfigure :inhaling 0.94ml/s @16:47:06.14 SS.seek 25ml @16:47:18.22 Vacuum too high -- waiting up to 40 seconds @16:47:18.23 SS.reconfigure :exhale @16:47:18.28 SS.hold @16:47:25.07 Intake @13.4psia, vacuum from 7.9 to 10.9psi @16:47:25.09 SS.reconfigure :inhaling 0.85ml/s @16:47:25.13 SS.seek 25ml @16:47:33.63 Vacuum too high -- waiting up to 40 seconds @16:47:33.64 SS.reconfigure :exhale @16:47:33.69 SS.hold @16:47:41.25 Intake @13.5psia, vacuum from 8.0 to 11.0psi @16:47:41.27 SS.reconfigure :inhaling 0.77ml/s @16:47:41.32 SS.seek 25ml @16:47:51.09 Vacuum too high -- waiting up to 40 seconds @16:47:51.11 SS.reconfigure :exhale @16:47:51.16 SS.hold @16:47:58.93 Intake @13.9psia, vacuum from 8.4 to 11.4psi @16:47:58.95 SS.reconfigure :inhaling 0.70ml/s @16:47:58.99 SS.seek 25ml @16:48:00.43 Stroke completed -- waiting for pressure to equalize ... @16:48:00.44 SS.reconfigure :exhale @16:48:00.49 SS.hold @16:48:06.11 Intake.select :close @16:48:06.26 SSV.dial :exhaust @16:48:07.03 Exhaust.select :open @16:48:07.14 SS.seek 0ml @16:48:16.46 Exhaust.select :close @16:48:16.57 SSV.dial CBV @16:48:16.60
CBV.dial SSV @16:48:17.38 Allowing 130s for 25.0ml of remaining 475.0ml @16:48:17.40 Intake.select :open @16:48:19.56 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:48:19.58 SS.reconfigure :inhaling 0.70ml/s @16:48:19.63 SS.seek 25ml @16:48:39.00 Vacuum too high -- waiting up to 40 seconds @16:48:39.01 SS.reconfigure :exhale @16:48:39.48 SS.hold @16:48:44.44 Intake @13.3psia, vacuum from 7.8 to 10.8psi @16:48:44.45 SS.reconfigure :inhaling 0.62ml/s @16:48:44.50 SS.seek 25ml @16:48:54.56 Vacuum too high -- waiting up to 40 seconds @16:48:54.57 SS.reconfigure :exhale @16:48:54.63 SS.hold @16:49:02.19 Intake @13.5psia, vacuum from 8.0 to 11.0psi @16:49:02.21 SS.reconfigure :inhaling 0.56ml/s @16:49:02.26 SS.seek 25ml @16:49:13.96 Stroke completed -- waiting for pressure to equalize ... @16:49:13.97 SS.reconfigure :exhale @16:49:14.01 SS.hold @16:49:25.45 Intake.select :close @16:49:25.60 SSV.dial :exhaust @16:49:26.37 Exhaust.select :open @16:49:26.48 SS.seek 0ml @16:49:35.93 Exhaust.select :close @16:49:36.04 SSV.dial CBV @16:49:36.06
CBV.dial SSV @16:49:36.88 Allowing 130s for 25.0ml of remaining 450.0ml @16:49:36.89 Intake.select :open @16:49:39.05 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:49:39.07 SS.reconfigure :inhaling 0.56ml/s @16:49:39.12 SS.seek 25ml @16:50:07.78 Vacuum too high -- waiting up to 40 seconds @16:50:07.79 SS.reconfigure :exhale @16:50:07.84 SS.hold @16:50:13.57 Intake @13.3psia, vacuum from 7.8 to 10.8psi @16:50:13.59 SS.reconfigure :inhaling 0.51ml/s @16:50:13.64 SS.seek 25ml @16:50:33.28 Stroke completed -- waiting for pressure to equalize ... @16:50:33.30 SS.reconfigure :exhale @16:50:33.34 SS.hold @16:50:47.71 Intake.select :close @16:50:47.86 SSV.dial :exhaust @16:50:48.65 Exhaust.select :open @16:50:48.76 SS.seek 0ml @16:50:58.24 Exhaust.select :close @16:50:58.35 SSV.dial CBV @16:50:58.37
CBV.dial SSV @16:50:59.16 Allowing 130s for 25.0ml of remaining 425.0ml @16:50:59.17 Intake.select :open @16:51:01.33 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:51:01.35 SS.reconfigure :inhaling 0.51ml/s @16:51:01.40 SS.seek 25ml @16:51:39.62 Vacuum too high -- waiting up to 40 seconds @16:51:39.63 SS.reconfigure :exhale @16:51:40.09 SS.hold @16:51:45.83 Intake @13.5psia, vacuum from 8.0 to 11.0psi @16:51:45.85 SS.reconfigure :inhaling 0.46ml/s @16:51:45.90 SS.seek 25ml @16:52:00.05 Stroke completed -- waiting for pressure to equalize ... @16:52:00.06 SS.reconfigure :exhale @16:52:00.11 SS.hold @16:52:16.39 Intake.select :close @16:52:16.55 SSV.dial :exhaust @16:52:17.32 Exhaust.select :open @16:52:17.43 SS.seek 0ml @16:52:26.85 Exhaust.select :close @16:52:26.96 SSV.dial CBV @16:52:26.99
CBV.dial SSV @16:52:27.77 Allowing 130s for 25.0ml of remaining 400.0ml @16:52:27.78 Intake.select :open @16:52:29.95 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:52:29.97 SS.reconfigure :inhaling 0.46ml/s @16:52:30.02 SS.seek 25ml @16:53:00.96 Vacuum too high -- waiting up to 40 seconds @16:53:00.97 SS.reconfigure :exhale @16:53:01.03 SS.hold @16:53:07.63 Intake @13.8psia, vacuum from 8.3 to 11.3psi @16:53:07.64 SS.reconfigure :inhaling 0.42ml/s @16:53:07.69 SS.seek 25ml @16:53:31.96 Vacuum too high -- waiting up to 40 seconds @16:53:31.97 SS.reconfigure :exhale @16:53:32.03 SS.hold @16:53:40.78 Intake @14.0psia, vacuum from 8.5 to 11.5psi @16:53:40.80 SS.reconfigure :inhaling 0.38ml/s @16:53:40.85 SS.seek 25ml @16:53:45.14 Stroke completed -- waiting for pressure to equalize ... @16:53:45.15 SS.reconfigure :exhale @16:53:45.20 SS.hold @16:53:55.00 Intake.select :close @16:53:55.15 SSV.dial :exhaust @16:53:55.92 Exhaust.select :open @16:53:56.03 SS.seek 0ml @16:54:05.39 Exhaust.select :close @16:54:05.50 SSV.dial CBV @16:54:05.52
CBV.dial SSV @16:54:06.31 Allowing 130s for 25.0ml of remaining 375.0ml @16:54:06.33 Intake.select :open @16:54:08.92 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:54:08.93 SS.reconfigure :inhaling 0.38ml/s @16:54:08.98 SS.seek 25ml @16:55:10.74 Vacuum too high -- waiting up to 40 seconds @16:55:10.75 SS.reconfigure :exhale @16:55:10.81 SS.hold @16:55:17.39 Intake @13.9psia, vacuum from 8.4 to 11.4psi @16:55:17.41 SS.reconfigure :inhaling 0.33ml/s @16:55:17.46 SS.seek 25ml @16:55:26.61 Stroke completed -- waiting for pressure to equalize ... @16:55:26.62 SS.reconfigure :exhale @16:55:26.66 SS.hold @16:55:39.22 Intake.select :close @16:55:39.37 SSV.dial :exhaust @16:55:40.16 Exhaust.select :open @16:55:40.27 SS.seek 0ml @16:55:49.61 Exhaust.select :close @16:55:49.72 SSV.dial CBV @16:55:49.74
CBV.dial SSV @16:55:50.52 Allowing 130s for 25.0ml of remaining 350.0ml @16:55:50.53 Intake.select :open @16:55:52.70 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:55:52.71 SS.reconfigure :inhaling 0.33ml/s @16:55:52.76 SS.seek 25ml @16:57:11.99 Stroke completed -- waiting for pressure to equalize ... @16:57:12.01 SS.reconfigure :exhale @16:57:12.05 SS.hold @16:57:30.72 Intake.select :close @16:57:30.88 SSV.dial :exhaust @16:57:31.64 Exhaust.select :open @16:57:31.75 SS.seek 0ml @16:57:41.37 Exhaust.select :close @16:57:41.48 SSV.dial CBV @16:57:41.52
CBV.dial SSV @16:57:42.26 Allowing 130s for 25.0ml of remaining 325.0ml @16:57:42.28 Intake.select :open @16:57:44.44 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:57:44.46 SS.reconfigure :inhaling 0.33ml/s @16:57:44.51 SS.seek 25ml @16:59:02.90 Stroke completed -- waiting for pressure to equalize ... @16:59:02.91 SS.reconfigure :exhale @16:59:02.96 SS.hold @16:59:23.35 Intake.select :close @16:59:23.50 SSV.dial :exhaust @16:59:24.27 Exhaust.select :open @16:59:24.75 SS.seek 0ml @16:59:33.81 Exhaust.select :close @16:59:33.92 SSV.dial CBV @16:59:33.94
CBV.dial SSV @16:59:34.71 Allowing 130s for 25.0ml of remaining 300.0ml @16:59:34.73 Intake.select :open @16:59:36.89 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:59:36.91 SS.reconfigure :inhaling 0.33ml/s @16:59:36.96 SS.seek 25ml @17:00:56.15 Stroke completed -- waiting for pressure to equalize ... @17:00:56.16 SS.reconfigure :exhale @17:00:56.20 SS.hold @17:01:20.68 Intake.select :close @17:01:20.83 SSV.dial :exhaust @17:01:21.60 Exhaust.select :open @17:01:21.71 SS.seek 0ml @17:01:31.44 Exhaust.select :close @17:01:31.55 SSV.dial CBV @17:01:31.58
CBV.dial SSV @17:01:32.34 Allowing 130s for 25.0ml of remaining 275.0ml @17:01:32.35 Intake.select :open @17:01:34.52 Intake @15.2psia, vacuum from 9.7 to 12.7psi @17:01:34.53 SS.reconfigure :inhaling 0.33ml/s @17:01:34.58 SS.seek 25ml @17:02:54.05 Stroke completed -- waiting for pressure to equalize ... @17:02:54.06 SS.reconfigure :exhale @17:02:54.11 SS.hold @17:03:16.60 Intake.select :close @17:03:16.76 SSV.dial :exhaust @17:03:17.54 Exhaust.select :open @17:03:17.65 SS.seek 0ml @17:03:27.14 Exhaust.select :close @17:03:27.25 SSV.dial CBV @17:03:27.28
CBV.dial SSV @17:03:28.05 Allowing 130s for 25.0ml of remaining 250.0ml @17:03:28.41 Intake.select :open @17:03:30.65 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:03:30.66 SS.reconfigure :inhaling 0.33ml/s @17:03:30.71 SS.seek 25ml @17:04:30.76 Vacuum too high -- waiting up to 40 seconds @17:04:30.77 SS.reconfigure :exhale @17:04:30.83 SS.hold @17:04:42.09 Intake @14.8psia, vacuum from 9.3 to 12.3psi @17:04:42.11 SS.reconfigure :inhaling 0.30ml/s @17:04:42.15 SS.seek 25ml @17:05:03.78 Stroke completed -- waiting for pressure to equalize ... @17:05:03.79 SS.reconfigure :exhale @17:05:03.84 SS.hold @17:05:22.60 Intake.select :close @17:05:22.75 SSV.dial :exhaust @17:05:23.53 Exhaust.select :open @17:05:23.64 SS.seek 0ml @17:05:33.06 Exhaust.select :close @17:05:33.17 SSV.dial CBV @17:05:33.19
CBV.dial SSV @17:05:33.97 Allowing 130s for 25.0ml of remaining 225.0ml @17:05:33.98 Intake.select :open @17:05:36.14 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:05:36.16 SS.reconfigure :inhaling 0.30ml/s @17:05:36.21 SS.seek 25ml @17:07:00.79 Vacuum too high -- waiting up to 40 seconds @17:07:00.80 SS.reconfigure :exhale @17:07:00.85 SS.hold @17:07:13.77 Intake @14.2psia, vacuum from 8.7 to 11.7psi @17:07:13.79 SS.reconfigure :inhaling 0.28ml/s @17:07:13.83 SS.seek 25ml @17:07:18.59 Stroke completed -- waiting for pressure to equalize ... @17:07:18.60 SS.reconfigure :exhale @17:07:18.65 SS.hold @17:07:35.00 Intake.select :close @17:07:35.16 SSV.dial :exhaust @17:07:35.94 Exhaust.select :open @17:07:36.05 SS.seek 0ml @17:07:45.50 Exhaust.select :close @17:07:45.61 SSV.dial CBV @17:07:45.63
CBV.dial SSV @17:07:46.42 Allowing 130s for 25.0ml of remaining 200.0ml @17:07:46.43 Intake.select :open @17:07:49.02 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:07:49.04 SS.reconfigure :inhaling 0.28ml/s @17:07:49.09 SS.seek 25ml @17:09:26.89 Stroke completed -- waiting for pressure to equalize ... @17:09:26.90 SS.reconfigure :exhale @17:09:26.94 SS.hold @17:09:55.52 Intake.select :close @17:09:55.68 SSV.dial :exhaust @17:09:56.45 Exhaust.select :open @17:09:56.56 SS.seek 0ml @17:10:06.07 Exhaust.select :close @17:10:06.18 SSV.dial CBV @17:10:06.59
CBV.dial SSV @17:10:07.42 Allowing 130s for 25.0ml of remaining 175.0ml @17:10:07.43 Intake.select :open @17:10:09.60 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:10:09.61 SS.reconfigure :inhaling 0.28ml/s @17:10:09.66 SS.seek 25ml @17:11:48.27 Stroke completed -- waiting for pressure to equalize ... @17:11:48.28 SS.reconfigure :exhale @17:11:48.33 SS.hold @17:12:16.92 Intake.select :close @17:12:17.08 SSV.dial :exhaust @17:12:17.86 Exhaust.select :open @17:12:17.97 SS.seek 0ml @17:12:27.88 Exhaust.select :close @17:12:27.99 SSV.dial CBV @17:12:28.01
CBV.dial SSV @17:12:28.81 Allowing 130s for 25.0ml of remaining 150.0ml @17:12:28.82 Intake.select :open @17:12:30.99 Intake @15.5psia, vacuum from 10.0 to 13.0psi @17:12:31.01 SS.reconfigure :inhaling 0.28ml/s @17:12:31.06 SS.seek 25ml @17:14:11.16 Stroke completed -- waiting for pressure to equalize ... @17:14:11.17 SS.reconfigure :exhale @17:14:11.22 SS.hold @17:14:38.83 SS.reconfigure :default @17:14:38.88 SS.hold @17:14:38.95 Intake.select :close @17:14:39.11 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 875.0ml @17:14:39.48 SSV.dial :exhaust @17:14:40.32 Exhaust.select :open @17:14:40.43 SS.reconfigure :exhale @17:14:40.48 SS.seek 0ml @17:14:50.10 Exhaust.select :close @17:14:50.21 Sampled 875.0ml @17:14:50.23 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @17:14:50.30 CSV.dial :waste @17:14:50.45 SSV.dial CBV,avoiding: :exhaust @17:14:50.48 CS.seek 0ml @17:14:50.51
CBV.dial SSV @17:14:50.60 CSV.dial :air @17:14:51.35 CS.seek 10ml @17:14:52.44
CTV.dial :air @17:14:53.22 delay 15 seconds @17:15:08.24 SSV.dial :spit,avoiding: :exhaust @17:15:09.60 CTV.dial CSV,avoiding: :intake @17:15:09.64
SS.seek 0ml @17:15:09.76 SSV.dial CBV,avoiding: :exhaust @17:15:15.80 SSV.dial between CBV and plug @17:15:16.29 CBV.dial between SSV and waste2 @17:15:16.68 CSV.dial between air and CTV @17:15:16.99 CS.seek 8.385ml @17:15:21.52 CTV.dial CSV,avoiding: :air @17:15:21.54
CSV.dial CTV @17:15:21.85 CS.reconfigure :pressurize @17:15:21.90 CS.seek 0ml @17:15:40.14 BubblePoint: Intake @24.2psia, Outlet @0.6psia @17:15:40.15 CBV.dial SSV @17:15:40.50 SS.reconfigure :default @17:15:40.55 SS.seek 10ml @17:15:46.07 delay 15 seconds @17:16:01.20 Evacuated: Intake @22.9psia, Outlet @21.9psia @17:16:01.21 SSV.dial :spit,avoiding: :exhaust @17:16:02.28 SS.reconfigure :exhale @17:16:02.32 SS.seek 0ml @17:16:07.35 SS.reconfigure :fast @17:16:07.40 SS.seek 5ml @17:16:10.20 SSV.dial CBV,avoiding: :exhaust @17:16:10.22
CBV.dial SSV @17:16:11.62 CTV.dial :air,avoiding: :intake @17:16:13.00 CSV.dial CTV @17:16:13.05
SS.seek 10ml @17:16:14.16 CSV.dial :air @17:16:14.62 CS.reconfigure :fast @17:16:14.67 CS.seek 0ml @17:16:15.57
SSV.dial :spit,avoiding: :exhaust @17:16:16.92 SS.reconfigure :exhale @17:16:16.96 SS.seek 0ml @17:16:19.32 CS.stop @17:16:22.26
SS.reconfigure :fast @17:16:22.31 SS.seek 5ml @17:16:24.98 SSV.dial CBV,avoiding: :exhaust @17:16:25.00
CBV.dial SSV @17:16:27.40 SSV.dial :spit,avoiding: :exhaust @17:16:28.73 SS.reconfigure :default @17:16:28.77 SS.seek 0ml @17:16:31.99 SS.stop @17:16:32.03 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @17:16:32.04 Intake.select :close @17:16:32.11 CSV.dial :waste @17:16:32.23 SSV.dial CBV,avoiding: :exhaust @17:16:32.27
CBV.dial SSV @17:16:32.86 CS.seek 0ml @17:16:32.92 CSV.dial :air @17:16:33.66
CTV.dial :air @17:16:33.74 CS.seek 10ml @17:16:33.80
delay 15 seconds @17:16:48.82 SSV.dial :spit,avoiding: :exhaust @17:16:50.16 CTV.dial CSV,avoiding: :intake @17:16:50.20
SS.reconfigure :exhale @17:16:50.30 SS.seek 0ml @17:16:50.36 SSV.dial CBV,avoiding: :exhaust @17:16:57.76 SSV.dial between CBV and plug @17:16:58.26 CBV.dial between SSV and waste2 @17:16:58.65 CSV.dial between air and CTV @17:16:58.96 CS.seek 7.811ml @17:17:04.83 CTV.dial CSV,avoiding: :air @17:17:04.85
CSV.dial CTV @17:17:05.17 CS.reconfigure :pressurize @17:17:05.22 CS.seek 0ml @17:17:22.89 BubblePoint: Intake @24.2psia, Outlet @1.0psia @17:17:22.90 CBV.dial SSV @17:17:23.25 SS.reconfigure :default @17:17:23.30 SS.seek 10ml @17:17:28.80 delay 15 seconds @17:17:43.93 Evacuated: Intake @23.8psia, Outlet @11.2psia @17:17:43.94 SSV.dial :spit,avoiding: :exhaust @17:17:45.01 SS.reconfigure :exhale @17:17:45.05 SS.seek 0ml @17:17:50.43 SS.reconfigure :fast @17:17:50.48 SS.seek 5ml @17:17:53.05 SSV.dial CBV,avoiding: :exhaust @17:17:53.08
CBV.dial SSV @17:17:54.46 CTV.dial :air,avoiding: :intake @17:17:55.84 CSV.dial CTV @17:17:55.89
SS.seek 10ml @17:17:57.00 CSV.dial :air @17:17:57.45 CS.reconfigure :fast @17:17:57.50 CS.seek 0ml @17:17:58.41
SSV.dial :spit,avoiding: :exhaust @17:17:59.76 SS.reconfigure :exhale @17:17:59.81 SS.seek 0ml @17:18:00.95 CS.stop @17:18:04.92
SS.reconfigure :fast @17:18:04.97 SS.seek 5ml @17:18:07.74 SSV.dial CBV,avoiding: :exhaust @17:18:07.77
CBV.dial SSV @17:18:10.15 SSV.dial :spit,avoiding: :exhaust @17:18:11.49 SS.reconfigure :default @17:18:11.54 SS.seek 0ml @17:18:14.77 SS.stop @17:18:14.80 Lysing: Adding 2.0ml AqMeOH @17:18:14.81 CBV.dial between CSV and waste1,avoiding: :DBV @17:18:16.32 CTV.dial CSV @17:18:17.68 CSV.dial CSR @17:18:18.47 Pulling 1ml of CSR.AqMeOH @17:18:18.48 CSR.select :AqMeOH @17:18:18.60 CS.reconfigure :default @17:18:18.64 CS.seek 1ml @17:18:22.04 delay 5 seconds @17:18:27.06 CSR.select :air @17:18:27.23 CS.seek 6ml @17:18:40.79 delay 2 seconds @17:18:42.81 CSV.dial :waste2 @17:18:43.25 CS.reconfigure :fast @17:18:43.30 CS.seek 0ml @17:18:57.86 CSV.dial CSR @17:18:58.31 Pulling 2ml of CSR.AqMeOH @17:18:58.32 CSR.select :AqMeOH @17:18:58.43 CS.reconfigure :default @17:18:58.48 CS.seek 2ml @17:19:04.42 delay 5 seconds @17:19:09.43 CSR.select :air @17:19:09.61 CS.seek 4.999ml @17:19:18.17 delay 2 seconds @17:19:20.18 CSV.dial CTV @17:19:20.62 CS.seek 1ml @17:19:32.18 Before lysing Collection Puck Heater at 17.1C/16.4psia @17:19:32.20 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @17:19:32.22 CH.seek 70C,8:00 @17:21:57.57 CH.hold 10:00,70C @17:21:57.77 Begun lysing Collection Puck Heater at 68.1C/22.8psia @17:31:57.78 Ended lysing Collection Puck Heater at 70C/20.0psia @17:31:57.80 CS.seek 0ml @17:32:01.26 CTV.dial between spare and air,avoiding: :intake @17:32:02.31 CSV.dial :air @17:32:02.76 CS.seek 5ml @17:32:16.44 CSV.dial CTV @17:32:16.89 delay 2 seconds @17:32:18.91 CSV.dial :air @17:32:19.36 delay 2 seconds @17:32:21.38 CSV.dial CTV @17:32:21.83 delay 2 seconds @17:32:23.85 CSV.dial :air @17:32:24.30 delay 2 seconds @17:32:26.32 CS.seek 0ml @17:32:40.02 Puck @28.2psia after final lysis pressurization @17:32:40.04 Recovering Lysate into Collection syringe @17:32:40.05 CSV.dial CBV @17:32:41.05 CBV.dial CSV @17:32:41.44 CS.seek 5ml @17:32:55.09 delay 5 seconds @17:33:00.11 CTV.dial :air @17:33:00.65 CS.seek 7.001ml @17:33:06.69 delay 5 seconds @17:33:11.71 CSV.dial :air2 @17:33:12.16 CS.seek 10ml @17:33:20.72 CSV.dial between air and CTV,avoiding: CBV @17:33:21.86 Lysing Complete @17:33:21.93 Storing sh1 puck in Collection Clamp to tube 2 @17:33:22.56 SC.reconfigure :tight @17:33:22.61 SC.seek 2 @17:33:26.36 SC.configure :limp @17:33:26.58 Elbow.reconfigure :tight @17:33:26.62 Elbow.seek 2 @17:33:28.32 Forearm.reconfigure :tight @17:33:28.37 Forearm.seek 2 + 800 counts @17:33:29.44 SE.reconfigure :pick @17:33:29.50 SE.seek :beamBreak @17:33:31.68 SE.configure :default @17:33:31.84 Elbow.reconfigure :limp @17:33:31.89 Forearm.reconfigure :loose @17:33:31.94 Forearm.seek 2 @17:33:32.38 Elbow.hold @17:33:32.45 Elbow.reconfigure :tight @17:33:32.55 SE.seek 9.08 Pucks below Top Plate @17:33:33.11 Forearm.force -20 @17:33:33.15 Forearm.seek 2 + 314 counts @17:33:33.41 Forearm.reconfigure :tight @17:33:33.46 Forearm.seek 2 + 800 counts @17:33:33.70 SE.seek 10.08 Pucks below Top Plate @17:33:34.39 Forearm.reconfigure :loose @17:33:34.43 Forearm.seek :retract @17:33:35.30 Elbow.seek CC @17:33:36.96 Elbow.reconfigure :loose @17:33:37.06 Forearm.seek CC @17:33:38.03 Hand.close @17:33:38.80 CC.seek :open @17:33:43.46 Forearm.seek :retract @17:33:44.44 Elbow.reconfigure :tight @17:33:44.49 Elbow.seek 2 @17:33:46.19 Elbow.reconfigure :limp @17:33:46.24 Forearm.seek 2 @17:33:47.38 Elbow.hold @17:33:47.44 Elbow.reconfigure :tight @17:33:47.49 Hand.open @17:33:48.29 Forearm.force -20 @17:33:48.33 Forearm.seek 2 + 317 counts @17:33:48.71 Forearm.reconfigure :tight @17:33:48.76 Forearm.seek 2 + 800 counts @17:33:49.05 SE.seek :down @17:33:50.19
Forearm.reconfigure :loose @17:33:50.23 Forearm.seek :retract @17:33:50.79 SE.stop @17:33:51.18
Tranferring DA @17:33:51.19 Beginning samplePassC @17:33:51.20 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:33:51.39 Elbow.seek FlushPuck::Garage @17:33:53.17 Elbow.reconfigure :loose @17:33:53.28 Forearm.seek :garage @17:33:54.22 Hand.close @17:33:55.04 Forearm.seek :retract @17:33:56.02 Elbow.reconfigure :tight @17:33:56.07 Elbow.seek CC @17:33:58.91 Elbow.reconfigure :loose @17:33:59.01 Forearm.reconfigure :tight @17:33:59.06 Forearm.seek CC @17:34:00.84 CC.seek :closed @17:34:04.82 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.96 @17:34:04.88 Hand.open @17:34:05.74 Forearm.reconfigure :loose @17:34:05.79 Forearm.seek :retract @17:34:06.75 PSV.dial PSR @17:34:08.19 Pulling 1.5ml of PSR.flush PSR.select :flush @17:34:08.31 PS.seek 1.5ml @17:34:24.39 delay 5 seconds @17:34:29.41 PSV.dial :waste1 @17:34:29.89 PS.seek 0ml @17:34:45.99 PSV.dial PSR @17:34:46.46 PSR.select :air @17:34:46.57 PS.seek 2.5ml @17:35:13.05 PRV.dial CBV,avoiding: :ab2 @17:35:13.08
CBV.dial PRV @17:35:14.83 PSV.dial :mixloop @17:35:15.86 CTV.dial :air,avoiding: :intake @17:35:15.94 PTV.dial :PRVmixing @17:35:17.23 PS.seek 0ml @17:35:43.55 PSV.dial :air @17:35:44.03 PS.seek 2.5ml @17:36:10.39 PSV.dial :mixloop @17:36:10.87 PS.seek 0ml @17:36:37.20 PSV.dial :air @17:36:37.68 PS.seek 2.5ml @17:37:04.02 PSV.dial :mixloop @17:37:04.49 PS.seek 0ml @17:37:30.86 Garaging FlushPuck in Collection Clamp @17:37:30.93 Elbow.reconfigure :tight @17:37:30.98 Elbow.seek CC @17:37:31.55 Elbow.reconfigure :loose @17:37:31.66 Forearm.seek CC @17:37:32.67 Hand.close @17:37:33.43 CC.seek :open @17:37:37.52 Forearm.seek :retract @17:37:38.50 Elbow.reconfigure :tight @17:37:38.55 Elbow.seek :garage @17:37:41.43 Elbow.reconfigure :loose @17:37:41.53 Forearm.reconfigure :tight @17:37:41.58 Forearm.seek :garage @17:37:42.73 Hand.open @17:37:43.48 Forearm.reconfigure :loose @17:37:43.53 Forearm.seek :retract @17:37:44.59 PSV.dial :mixloop @17:37:44.67 PTV.dial :PRVmixing @17:37:44.69 PRV.dial CBV @17:37:44.71
CBV.dial PRV @17:37:44.93 CTV.dial :air,avoiding: :intake @17:37:45.00 Loading lyfil puck from tube 3 into Collection Clamp @17:37:45.24 SC.reconfigure :tight @17:37:45.28 SC.seek 3 @17:37:48.84 SC.configure :limp @17:37:49.05 Elbow.reconfigure :tight @17:37:49.10 Elbow.seek 3 @17:37:51.10 Forearm.reconfigure :tight @17:37:51.15 Forearm.seek 3 + 800 counts @17:37:52.19 SE.reconfigure :pick @17:37:52.24 SE.seek :beamBreak @17:37:54.32 SE.configure :default @17:37:54.92 Elbow.seek 3 @17:37:55.11 Forearm.seek 3 + 300 counts @17:37:55.39 Forearm.reconfigure :loose @17:37:55.44 Hand.close @17:37:56.18 SE.seek :down @17:37:56.38
Forearm.seek :retract @17:37:57.36 Elbow.seek CC @17:37:58.06 SE.stop @17:37:59.01
Elbow.reconfigure :loose @17:37:59.11 Forearm.reconfigure :tight @17:37:59.16 Forearm.seek CC @17:38:00.41 CC.seek :closed @17:38:04.38 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.01 @17:38:04.39 Hand.open @17:38:05.24 Forearm.reconfigure :loose @17:38:05.29 Forearm.seek :retract @17:38:06.27 CTV.dial CSV,avoiding: :intake @17:38:07.62 CSV.dial CTV @17:38:08.07 PS.seek 2.5ml @17:38:34.55 CS.seek 5.001ml @17:38:48.12 delay 8 seconds @17:38:56.14 PSV.dial :air @17:38:56.61 delay 2 seconds @17:38:58.63 PSV.dial :waste2 @17:38:59.37 PS.seek 0ml @17:39:25.73 CBV.dial between PRV and waste1 @17:39:26.07 CSV.dial :air @17:39:26.95 CS.seek 10ml @17:39:40.56 CSV.dial CTV @17:39:41.02 CS.seek 0ml @17:40:07.37 CBV.dial :waste1 @17:40:07.68 delay 5 seconds @17:40:12.70 CTV.dial :air,avoiding: :intake @17:40:14.13 Storing lyfil puck in Collection Clamp to tube 2 @17:40:14.30 SC.reconfigure :tight @17:40:14.35 SC.seek 2 @17:40:18.16 SC.configure :limp @17:40:18.38 Elbow.reconfigure :tight @17:40:18.42 Elbow.seek 2 @17:40:20.13 Forearm.reconfigure :tight @17:40:20.18 Forearm.seek 2 + 800 counts @17:40:21.29 SE.reconfigure :pick @17:40:21.35 SE.seek :beamBreak @17:40:23.41 SE.configure :default @17:40:23.57 Elbow.reconfigure :limp @17:40:23.62 Forearm.reconfigure :loose @17:40:23.66 Forearm.seek 2 @17:40:24.04 Elbow.hold @17:40:24.11 Elbow.reconfigure :tight @17:40:24.21 SE.seek 10.09 Pucks below Top Plate @17:40:24.74 Forearm.force -20 @17:40:24.79 Forearm.seek 2 + 311 counts @17:40:25.05 Forearm.reconfigure :tight @17:40:25.10 Forearm.seek 2 + 800 counts @17:40:25.46 SE.seek 11.09 Pucks below Top Plate @17:40:26.41 Forearm.reconfigure :loose @17:40:26.52 Forearm.seek :retract @17:40:27.48 Elbow.seek CC @17:40:29.13 Elbow.reconfigure :loose @17:40:29.23 Forearm.seek CC @17:40:30.23 Hand.close @17:40:30.92 CC.seek :open @17:40:35.13 Forearm.seek :retract @17:40:36.11 Elbow.reconfigure :tight @17:40:36.16 Elbow.seek 2 @17:40:37.86 Elbow.reconfigure :limp @17:40:37.91 Forearm.seek 2 @17:40:39.04 Elbow.hold @17:40:39.11 Elbow.reconfigure :tight @17:40:39.16 Hand.open @17:40:40.04 Forearm.force -20 @17:40:40.09 Forearm.seek 2 + 319 counts @17:40:40.35 Forearm.reconfigure :tight @17:40:40.40 Forearm.seek 2 + 800 counts @17:40:40.70 SE.seek :down @17:40:41.84
Forearm.reconfigure :loose @17:40:41.89 Forearm.seek :retract @17:40:42.35 SE.stop @17:40:42.81
PSV.dial :air @17:40:43.57 PS.seek 2.5ml @17:41:09.86 delay 1 second @17:41:10.87 PSV.dial :mixloop @17:41:11.33 delay 1 second @17:41:12.35 PSV.dial :air @17:41:12.81 delay 1 second @17:41:13.83 PSV.dial :mixloop @17:41:14.74 delay 1 second @17:41:15.75 PSV.dial :air @17:41:16.22 delay 1 second @17:41:17.24 PS.seek 0ml @17:41:43.58 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:41:43.77 Elbow.seek FlushPuck::Garage @17:41:45.57 Elbow.reconfigure :loose @17:41:45.67 Forearm.seek :garage @17:41:46.67 Hand.close @17:41:47.54 Forearm.seek :retract @17:41:48.58 Elbow.reconfigure :tight @17:41:48.63 Elbow.seek PC @17:41:49.90 Elbow.reconfigure :loose @17:41:50.01 Forearm.reconfigure :tight @17:41:50.05 Forearm.seek PC @17:41:51.43 PC.seek :closed @17:41:55.87 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.56 @17:41:55.94 Hand.open @17:41:56.73 Forearm.reconfigure :loose @17:41:56.78 Forearm.seek :retract @17:41:57.78 PRV.dial :air @17:41:58.25 PSV.dial :mixloop @17:41:58.79 PS.seek 0.3ml @17:42:02.77 PSV.dial PSR @17:42:03.82 Pulling 0.4ml of PSR.air @17:42:03.83 PSR.select :air @17:42:03.85 PS.seek 0.7ml @17:42:09.27 delay 3 seconds @17:42:12.28 PSR.select :air @17:42:12.31 PS.seek 2.5ml @17:42:31.55 delay 3 seconds @17:42:34.56 PSV.dial :mixloop @17:42:35.64 PTV.dial :mixing @17:42:36.37 PBV.dial :waste2 @17:42:37.37 PS.seek 0ml @17:43:03.70 Garaging FlushPuck in Processing Clamp @17:43:03.78 Elbow.reconfigure :tight @17:43:03.82 Elbow.seek PC @17:43:03.94 Elbow.reconfigure :loose @17:43:04.05 Forearm.seek PC @17:43:05.07 Hand.close @17:43:05.80 PC.seek :open @17:43:10.29 Forearm.seek :retract @17:43:11.30 Elbow.reconfigure :tight @17:43:11.34 Elbow.seek :garage @17:43:12.74 Elbow.reconfigure :loose @17:43:12.84 Forearm.reconfigure :tight @17:43:12.88 Forearm.seek :garage @17:43:13.98 Hand.open @17:43:14.73 Forearm.reconfigure :loose @17:43:14.77 Forearm.seek :retract @17:43:15.80 Loading da puck from tube 3 into Processing Clamp @17:43:16.04 SC.reconfigure :tight @17:43:16.09 SC.seek 3 @17:43:19.69 SC.configure :limp @17:43:19.91 Elbow.reconfigure :tight @17:43:19.95 Elbow.seek 3 @17:43:21.85 Forearm.reconfigure :tight @17:43:21.90 Forearm.seek 3 + 800 counts @17:43:22.93 SE.reconfigure :pick @17:43:22.99 SE.seek :beamBreak @17:43:25.07 SE.configure :default @17:43:25.23 Elbow.seek 3 @17:43:25.42 Forearm.seek 3 + 300 counts @17:43:25.73 Forearm.reconfigure :loose @17:43:25.78 Hand.close @17:43:26.55 SE.seek :down @17:43:26.77
Forearm.seek :retract @17:43:27.76 Elbow.seek PC @17:43:28.47 SE.stop @17:43:30.36
Elbow.reconfigure :loose @17:43:30.46 Forearm.reconfigure :tight @17:43:30.51 Forearm.seek PC @17:43:31.90 PC.seek :closed @17:43:36.34 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.58 @17:43:36.35 Hand.open @17:43:37.11 Forearm.reconfigure :loose @17:43:37.15 Forearm.seek :retract @17:43:38.21 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @17:43:38.23 PH.seek 28C,5:00 @17:44:23.68 PH.hold 2:00:00,28C @17:44:23.73 delay 1:30 @17:45:53.76 PSR.select :air @17:45:53.78 PSV.dial PSR @17:45:54.86 Pulling 0.5ml of PSR.wash @17:45:54.87 PSR.select :wash @17:45:54.99 PS.seek 0.5ml @17:46:00.95 delay 5 seconds @17:46:05.97 PSR.select :air @17:46:06.09 PS.seek 2ml @17:46:22.26 PTV.dial :puck @17:46:23.01 PSV.dial PTV @17:46:23.78 PBV.dial :waste1 @17:46:25.25 PS.seek 1.5ml @17:46:31.14 delay 3 seconds @17:46:34.16 PBV.dial between PSV and waste1 @17:46:34.53 PS.seek 0ml @17:46:50.86 delay 5:00 @17:51:50.88 recoverPSRreagent08 @17:51:50.89 PTV.dial :puck @17:51:50.96 PBV.dial :waste1 @17:51:51.35 delay 30 seconds @17:52:21.38 PBV.dial between PSV and waste1 @17:52:21.77 PSR.select :air @17:52:22.17 PSV.dial PSR @17:52:23.00 PS.reconfigure :fast @17:52:23.05 PS.seek 2.5ml @17:52:46.85 PSV.dial PTV @17:52:47.64 delay 5 seconds @17:52:52.65 PSV.dial PSR @17:52:53.43 delay 5 seconds @17:52:58.45 PSV.dial PTV @17:52:59.21 delay 5 seconds @17:53:04.23 PSV.dial :air @17:53:05.02 PS.seek 0ml @17:53:28.51 PTV.dial :PRVmixing,via: PRV @17:53:29.84 PSV.dial :mixloop @17:53:30.32 PRV.dial :ab1 @17:53:31.09 PS.reconfigure :slow @17:53:31.14 PS.seek 0.15ml @17:53:34.66 delay 2 seconds @17:53:36.68 CBV.dial PRV @17:53:36.71
PRV.dial CBV @17:53:37.29 PS.seek 0.34ml @17:53:41.70 delay 2 seconds @17:53:43.71 CBV.dial between PRV and PSV @17:53:44.05 PRV.dial :air @17:53:44.52 PS.seek 0.9ml @17:53:56.51 PRV.dial :waste2 @17:53:57.27 PS.seek 0.5ml @17:54:05.98 addPMLReagent08 @17:54:05.99 PSV.dial :air @17:54:06.47 PS.reconfigure :default @17:54:06.51 PS.seek 2ml @17:54:22.72 PSV.dial :PML @17:54:23.21 PTV.dial :mixing @17:54:23.95 PBV.dial :waste1 @17:54:24.38 PS.seek 1.85ml @17:54:26.76 PBV.dial between PSV and waste1 @17:54:27.14 PS.seek 0ml @17:54:46.82 delay 2 seconds @17:54:48.84 Beginning samplePassC @17:54:48.85 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:54:49.04 Elbow.reconfigure :tight @17:54:49.08 Elbow.seek FlushPuck::Garage @17:54:50.51 Elbow.reconfigure :loose @17:54:50.61 Forearm.seek :garage @17:54:51.69 Hand.close @17:54:52.55 Forearm.seek :retract @17:54:53.53 Elbow.reconfigure :tight @17:54:53.58 Elbow.seek CC @17:54:56.40 Elbow.reconfigure :loose @17:54:56.50 Forearm.reconfigure :tight @17:54:56.55 Forearm.seek CC @17:54:57.83 CC.seek :closed @17:55:01.81 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.96 @17:55:01.87 Hand.open @17:55:02.73 Forearm.reconfigure :loose @17:55:02.77 Forearm.seek :retract @17:55:03.78 PSV.dial PSR @17:55:04.82 Pulling 1.5ml of PSR.flush @17:55:04.83 PSR.select :flush @17:55:04.95 PS.seek 1.5ml @17:55:21.13 delay 5 seconds @17:55:26.15 PSV.dial :waste1 @17:55:26.62 PS.seek 0ml @17:55:42.76 PSV.dial PSR @17:55:43.23 PSR.select :air @17:55:43.35 PS.seek 2.5ml @17:56:09.73 PRV.dial CBV,avoiding: :ab2 @17:56:09.75
CBV.dial PRV @17:56:11.41 PSV.dial :mixloop @17:56:12.45 CTV.dial :air,avoiding: :intake @17:56:12.53 PTV.dial :PRVmixing @17:56:13.28 PS.seek 0ml @17:56:39.69 PSV.dial :air @17:56:40.17 PS.seek 2.5ml @17:57:06.47 PSV.dial :mixloop @17:57:06.95 PS.seek 0ml @17:57:33.29 PSV.dial :air @17:57:33.76 PS.seek 2.5ml @17:58:00.13 PSV.dial :mixloop @17:58:00.61 PS.seek 0ml @17:58:27.01 Garaging FlushPuck in Collection Clamp @17:58:27.08 Elbow.reconfigure :tight @17:58:27.12 Elbow.seek CC @17:58:27.25 Elbow.reconfigure :loose @17:58:27.35 Forearm.seek CC @17:58:28.31 Hand.close @17:58:29.03 CC.seek :open @17:58:33.16 Forearm.seek :retract @17:58:34.20 Elbow.reconfigure :tight @17:58:34.25 Elbow.seek :garage @17:58:37.08 Elbow.reconfigure :loose @17:58:37.18 Forearm.reconfigure :tight @17:58:37.23 Forearm.seek :garage @17:58:38.36 Hand.open @17:58:39.21 Forearm.reconfigure :loose @17:58:39.26 Forearm.seek :retract @17:58:40.33 delayUntil "18:02:48.83" @17:58:40.36 Whole Cell Archive sampling 1000ml @17:58:40.37 Loading wcr puck from tube 3 into Collection Clamp @17:58:40.61 SC.reconfigure :tight @17:58:40.66 SC.seek 3 @17:58:40.74 SC.configure :limp @17:58:40.96 Elbow.reconfigure :tight @17:58:41.01 Elbow.seek 3 @17:58:42.96 Forearm.reconfigure :tight @17:58:43.01 Forearm.seek 3 + 800 counts @17:58:44.43 SE.reconfigure :pick @17:58:44.49 SE.seek :beamBreak @17:58:46.76 SE.configure :default @17:58:46.93 Elbow.seek 3 @17:58:47.26 Forearm.seek 3 + 300 counts @17:58:47.55 Forearm.reconfigure :loose @17:58:47.59 Hand.close @17:58:48.29 SE.seek :down @17:58:48.51 Forearm.seek :retract @17:58:49.47 Elbow.seek CC @17:58:50.34 SE.stop @17:58:51.07 Elbow.reconfigure :loose @17:58:51.18 Forearm.reconfigure :tight @17:58:51.23 Forearm.seek CC @17:58:52.50 CC.seek :closed @17:58:56.50 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.91 @17:58:56.51 Hand.open @17:58:57.34 Forearm.reconfigure :loose @17:58:57.39 Forearm.seek :retract @17:58:58.41 collectSample 1000ml w/bubble point of 35psi @17:58:58.42 Priming 7ml @17:58:58.43 Intake.select :close @17:58:58.44 Exhaust.select :close @17:58:58.45 SS.hold @17:58:59.13 Calibrating pressure sensors @17:58:59.15 CTV.dial :air @17:58:59.16 SSV.dial :spit,avoiding: :exhaust @17:58:59.25 SS.reconfigure :fast @17:58:59.30 SS.seek 5ml @17:59:01.96 SSV.dial CBV @17:59:02.00 CBV.dial SSV @17:59:03.38 delay 3.8 seconds @17:59:07.31 Sensor limits: Intake max 104.1psia; Outlet max 110.7psia @17:59:07.33 SSV.dial :spit @17:59:08.68 SS.seek 0ml @17:59:11.55 SSV.dial CBV @17:59:12.90 CTV.dial :intake @17:59:13.67 SSV.dial CBV @17:59:13.71 CBV.dial SSV @17:59:13.84 Allowing 40s for 7.0ml of remaining 7.0ml @17:59:13.85 Intake.select :open @17:59:16.02 Intake @14.2psia, vacuum from 8.7 to 11.7psi @17:59:16.04 SS.reconfigure :inhaling 2.17ml/s @17:59:16.09 SS.seek 7ml @17:59:21.92 Stroke completed -- waiting for pressure to equalize ... @17:59:21.94 SS.reconfigure :exhale @17:59:21.98 SS.hold @17:59:33.65 Intake.select :close @17:59:33.81 SSV.dial :exhaust @17:59:34.58 Exhaust.select :open @17:59:34.70 SS.seek 0ml @17:59:39.08 Exhaust.select :close @17:59:39.19 Sampling 1000.0ml @17:59:39.21 Intake.select :close @17:59:39.22 Exhaust.select :close @17:59:39.23 SS.reconfigure :default @17:59:39.28 SS.hold @17:59:39.49 CTV.dial :intake @17:59:39.51 SSV.dial CBV @17:59:39.54 CBV.dial SSV @17:59:40.32 Allowing 130s for 25.0ml of remaining 1000.0ml @17:59:40.33 Intake.select :open @17:59:42.50 Intake @13.9psia, vacuum from 8.4 to 11.4psi @17:59:42.52 SS.reconfigure :inhaling 2.17ml/s @17:59:42.57 SS.seek 25ml @17:59:54.04 Vacuum too high -- waiting up to 40 seconds @17:59:54.06 SS.reconfigure :exhale @17:59:54.11 SS.hold @18:00:00.13 Intake @11.9psia, vacuum from 6.4 to 9.4psi @18:00:00.14 SS.reconfigure :inhaling 1.95ml/s @18:00:00.19 SS.seek 25ml @18:00:03.56 Stroke completed -- waiting for pressure to equalize ... @18:00:03.58 SS.reconfigure :exhale @18:00:03.62 SS.hold @18:00:13.41 Intake.select :close @18:00:13.56 SSV.dial :exhaust @18:00:14.34 Exhaust.select :open @18:00:14.45 SS.seek 0ml @18:00:23.69 Exhaust.select :close @18:00:23.80 SSV.dial CBV @18:00:23.84 CBV.dial SSV @18:00:24.61 Allowing 130s for 25.0ml of remaining 975.0ml @18:00:24.63 Intake.select :open @18:00:26.79 Intake @13.7psia, vacuum from 8.2 to 11.2psi @18:00:26.81 SS.reconfigure :inhaling 1.95ml/s @18:00:26.86 SS.seek 25ml @18:00:34.20 Vacuum too high -- waiting up to 40 seconds @18:00:34.21 SS.reconfigure :exhale @18:00:34.27 SS.hold @18:00:35.18 Intake @11.5psia, vacuum from 6.0 to 9.0psi @18:00:35.20 SS.reconfigure :inhaling 1.77ml/s @18:00:35.25 SS.seek 25ml @18:00:38.26 Vacuum too high -- waiting up to 40 seconds @18:00:38.28 SS.reconfigure :exhale @18:00:38.33 SS.hold @18:00:44.25 Intake @12.5psia, vacuum from 7.0 to 10.0psi @18:00:44.27 SS.reconfigure :inhaling 1.59ml/s @18:00:44.31 SS.seek 25ml @18:00:50.82 Vacuum too high -- waiting up to 40 seconds @18:00:50.84 SS.reconfigure :exhale @18:00:50.89 SS.hold @18:00:55.09 Intake @12.3psia, vacuum from 6.8 to 9.8psi @18:00:55.11 SS.reconfigure :inhaling 1.43ml/s @18:00:55.16 SS.seek 25ml @18:00:57.22 Stroke completed -- waiting for pressure to equalize ... @18:00:57.23 SS.reconfigure :exhale @18:00:57.28 SS.hold @18:01:01.45 Intake.select :close @18:01:01.61 SSV.dial :exhaust @18:01:02.38 Exhaust.select :open @18:01:02.50 SS.seek 0ml @18:01:11.85 Exhaust.select :close @18:01:11.96 SSV.dial CBV @18:01:12.00 CBV.dial SSV @18:01:12.77 Allowing 130s for 25.0ml of remaining 950.0ml @18:01:12.78 Intake.select :open @18:01:14.94 Intake @13.9psia, vacuum from 8.4 to 11.4psi @18:01:14.96 SS.reconfigure :inhaling 1.43ml/s @18:01:15.01 SS.seek 25ml @18:01:25.32 Vacuum too high -- waiting up to 40 seconds @18:01:25.34 SS.reconfigure :exhale @18:01:25.39 SS.hold @18:01:26.74 Intake @11.6psia, vacuum from 6.1 to 9.1psi @18:01:26.76 SS.reconfigure :inhaling 1.29ml/s @18:01:26.80 SS.seek 25ml @18:01:30.23 Vacuum too high -- waiting up to 40 seconds @18:01:30.24 SS.reconfigure :exhale @18:01:30.30 SS.hold @18:01:35.32 Intake @12.5psia, vacuum from 7.0 to 10.0psi @18:01:35.34 SS.reconfigure :inhaling 1.16ml/s @18:01:35.39 SS.seek 25ml @18:01:43.73 Stroke completed -- waiting for pressure to equalize ... @18:01:43.75 SS.reconfigure :exhale @18:01:43.80 SS.hold @18:01:51.27 Intake.select :close @18:01:51.43 SSV.dial :exhaust @18:01:52.19 Exhaust.select :open @18:01:52.30 SS.seek 0ml @18:02:01.22 Exhaust.select :close @18:02:01.33 SSV.dial CBV @18:02:01.36 CBV.dial SSV @18:02:02.16 Allowing 130s for 25.0ml of remaining 925.0ml @18:02:02.17 Intake.select :open @18:02:04.34 Intake @13.9psia, vacuum from 8.4 to 11.4psi @18:02:04.35 SS.reconfigure :inhaling 1.16ml/s @18:02:04.40 SS.seek 25ml @18:02:20.35 Vacuum too high -- waiting up to 40 seconds @18:02:20.37 SS.reconfigure :exhale @18:02:20.42 SS.hold @18:02:22.53 Intake @11.8psia, vacuum from 6.3 to 9.3psi @18:02:22.55 SS.reconfigure :inhaling 1.04ml/s @18:02:22.60 SS.seek 25ml @18:02:31.64 Stroke completed -- waiting for pressure to equalize ... @18:02:31.65 SS.reconfigure :exhale @18:02:31.70 SS.hold @18:02:39.39 Intake.select :close @18:02:39.55 SSV.dial :exhaust @18:02:40.31 Exhaust.select :open @18:02:40.43 SS.seek 0ml @18:02:48.84 recoverPRVreagent08 @18:02:48.85 PTV.dial :mixing @18:02:49.60 PBV.dial :waste1 @18:02:49.91 Exhaust.select :close @18:02:49.99 delay 30 seconds @18:02:50.02 SSV.dial CBV @18:02:50.06 CBV.dial SSV @18:02:50.84 Allowing 130s for 25.0ml of remaining 900.0ml @18:02:50.86 Intake.select :open @18:02:53.02 Intake @13.7psia, vacuum from 8.2 to 11.2psi @18:02:53.04 SS.reconfigure :inhaling 1.04ml/s @18:02:53.09 SS.seek 25ml @18:03:08.98 Vacuum too high -- waiting up to 40 seconds @18:03:08.99 SS.reconfigure :exhale @18:03:09.04 SS.hold @18:03:12.07 Intake @12.0psia, vacuum from 6.5 to 9.5psi @18:03:12.09 SS.reconfigure :inhaling 0.94ml/s @18:03:12.14 SS.seek 25ml @18:03:20.01 PBV.dial between PSV and waste1 @18:03:20.39 PSV.dial :air @18:03:20.88 PS.reconfigure :fast @18:03:20.93 PS.seek 2.5ml @18:03:23.31 Stroke completed -- waiting for pressure to equalize ... @18:03:23.32 SS.reconfigure :exhale @18:03:23.37 SS.hold @18:03:29.88 Intake.select :close @18:03:30.04 SSV.dial :exhaust @18:03:30.82 Exhaust.select :open @18:03:30.93 SS.seek 0ml @18:03:40.16 Exhaust.select :close @18:03:40.27 SSV.dial CBV @18:03:40.30 CBV.dial SSV @18:03:41.11 Allowing 130s for 25.0ml of remaining 875.0ml @18:03:41.12 Intake.select :open @18:03:43.28 Intake @14.0psia, vacuum from 8.5 to 11.5psi @18:03:43.30 SS.reconfigure :inhaling 0.94ml/s @18:03:43.35 SS.seek 25ml @18:03:45.32 PSV.dial :mixloop @18:03:45.80 delay 2 seconds @18:03:47.82 PSV.dial :air @18:03:48.72 delay 2 seconds @18:03:50.74 PSV.dial :mixloop @18:03:51.21 delay 2 seconds @18:03:53.22 PSV.dial :air @18:03:53.70 PS.seek 0ml @18:04:08.10 Vacuum too high -- waiting up to 40 seconds @18:04:08.12 SS.reconfigure :exhale @18:04:08.17 SS.hold @18:04:10.74 Intake @12.5psia, vacuum from 7.0 to 10.0psi @18:04:10.76 SS.reconfigure :inhaling 0.85ml/s @18:04:10.81 SS.seek 25ml @18:04:15.20 Stroke completed -- waiting for pressure to equalize ... @18:04:15.21 SS.reconfigure :exhale @18:04:15.26 SS.hold @18:04:17.94 washCycleDA #1 of 2 @18:04:17.95 PSR.select :air @18:04:17.97 PSV.dial PSR @18:04:19.31 Pulling 0.5ml of PSR.wash @18:04:19.32 PSR.select :wash @18:04:19.51 PS.reconfigure :default @18:04:19.56 PS.seek 0.5ml @18:04:22.30 Intake.select :close @18:04:22.46 SSV.dial :exhaust @18:04:23.22 Exhaust.select :open @18:04:23.34 SS.seek 0ml @18:04:25.53 delay 5 seconds @18:04:30.55 PSR.select :air @18:04:30.67 PS.seek 2ml @18:04:32.26 Exhaust.select :close @18:04:32.38 SSV.dial CBV @18:04:32.41 CBV.dial SSV @18:04:33.20 Allowing 130s for 25.0ml of remaining 850.0ml @18:04:33.22 Intake.select :open @18:04:35.39 Intake @13.9psia, vacuum from 8.4 to 11.4psi @18:04:35.40 SS.reconfigure :inhaling 0.85ml/s @18:04:35.45 SS.seek 25ml @18:04:46.78 PTV.dial :puck @18:04:47.53 PSV.dial PTV @18:04:48.28 PBV.dial :waste1 @18:04:48.73 PS.seek 1.5ml @18:04:54.72 delay 3 seconds @18:04:57.74 PBV.dial between PSV and waste1 @18:04:58.13 PS.seek 0ml @18:05:06.65 Stroke completed -- waiting for pressure to equalize ... @18:05:06.67 SS.reconfigure :exhale @18:05:06.71 SS.hold @18:05:14.25 delay 2:00 @18:05:15.31 Intake.select :close @18:05:15.47 SSV.dial :exhaust @18:05:16.23 Exhaust.select :open @18:05:16.35 SS.seek 0ml @18:05:25.72 Exhaust.select :close @18:05:25.83 SSV.dial CBV @18:05:25.87 CBV.dial SSV @18:05:26.64 Allowing 130s for 25.0ml of remaining 825.0ml @18:05:26.65 Intake.select :open @18:05:28.82 Intake @14.0psia, vacuum from 8.5 to 11.5psi @18:05:28.84 SS.reconfigure :inhaling 0.85ml/s @18:05:28.89 SS.seek 25ml @18:05:50.57 Vacuum too high -- waiting up to 40 seconds @18:05:50.58 SS.reconfigure :exhale @18:05:50.64 SS.hold @18:05:52.80 Intake @12.1psia, vacuum from 6.6 to 9.6psi @18:05:52.82 SS.reconfigure :inhaling 0.77ml/s @18:05:52.87 SS.seek 25ml @18:06:03.10 Stroke completed -- waiting for pressure to equalize ... @18:06:03.12 SS.reconfigure :exhale @18:06:03.17 SS.hold @18:06:08.99 Intake.select :close @18:06:09.15 SSV.dial :exhaust @18:06:09.92 Exhaust.select :open @18:06:10.04 SS.seek 0ml @18:06:19.44 Exhaust.select :close @18:06:19.55 SSV.dial CBV @18:06:19.58 CBV.dial SSV @18:06:20.39 Allowing 130s for 25.0ml of remaining 800.0ml @18:06:20.40 Intake.select :open @18:06:22.57 Intake @14.3psia, vacuum from 8.8 to 11.8psi @18:06:22.59 SS.reconfigure :inhaling 0.77ml/s @18:06:22.64 SS.seek 25ml @18:06:56.59 Stroke completed -- waiting for pressure to equalize ... @18:06:56.60 SS.reconfigure :exhale @18:06:56.65 SS.hold @18:07:07.83 Intake.select :close @18:07:08.00 SSV.dial :exhaust @18:07:08.78 Exhaust.select :open @18:07:08.90 SS.seek 0ml @18:07:14.27 recoverPSRreagent08 @18:07:14.28 PTV.dial :puck @18:07:14.36 PBV.dial :waste1 @18:07:14.75 delay 30 seconds @18:07:18.26 Exhaust.select :close @18:07:18.37 SSV.dial CBV @18:07:18.41 CBV.dial SSV @18:07:19.17 Allowing 130s for 25.0ml of remaining 775.0ml @18:07:19.18 Intake.select :open @18:07:21.34 Intake @14.5psia, vacuum from 9.0 to 12.0psi @18:07:21.36 SS.reconfigure :inhaling 0.77ml/s @18:07:21.41 SS.seek 25ml @18:07:44.77 PBV.dial between PSV and waste1 @18:07:45.15 PSR.select :air @18:07:45.17 PSV.dial PSR @18:07:46.05 PS.reconfigure :fast @18:07:46.10 PS.seek 2.5ml @18:07:49.72 Vacuum too high -- waiting up to 40 seconds @18:07:49.74 SS.reconfigure :exhale @18:07:49.80 SS.hold @18:07:53.89 Intake @13.2psia, vacuum from 7.7 to 10.7psi @18:07:53.91 SS.reconfigure :inhaling 0.70ml/s @18:07:53.96 SS.seek 25ml @18:08:00.54 Stroke completed -- waiting for pressure to equalize ... @18:08:00.55 SS.reconfigure :exhale @18:08:00.60 SS.hold @18:08:08.08 Intake.select :close @18:08:08.24 SSV.dial :exhaust @18:08:09.01 Exhaust.select :open @18:08:09.13 SS.seek 0ml @18:08:09.53 PSV.dial PTV @18:08:10.28 delay 5 seconds @18:08:15.30 PSV.dial PSR @18:08:16.06 delay 5 seconds @18:08:18.15 Exhaust.select :close @18:08:18.27 SSV.dial CBV @18:08:18.30 CBV.dial SSV @18:08:19.09 Allowing 130s for 25.0ml of remaining 750.0ml @18:08:19.10 Intake.select :open @18:08:21.08 PSV.dial PTV @18:08:21.26 Intake @14.4psia, vacuum from 8.9 to 11.9psi @18:08:21.28 SS.reconfigure :inhaling 0.70ml/s @18:08:21.33 SS.seek 25ml @18:08:21.86 delay 5 seconds @18:08:26.88 PSV.dial :air @18:08:27.68 PS.seek 0ml @18:08:51.09 washCycleDA #2 of 2 @18:08:51.10 PSR.select :air @18:08:51.12 PSV.dial PSR @18:08:52.45 Pulling 0.5ml of PSR.wash @18:08:52.46 PSR.select :wash @18:08:52.58 PS.reconfigure :default @18:08:52.63 PS.seek 0.5ml @18:08:58.53 delay 5 seconds @18:08:58.98 Stroke completed -- waiting for pressure to equalize ... @18:08:58.99 SS.reconfigure :exhale @18:08:59.04 SS.hold @18:09:03.54 PSR.select :air @18:09:03.67 PS.seek 2ml @18:09:11.72 Intake.select :close @18:09:11.88 SSV.dial :exhaust @18:09:12.65 Exhaust.select :open @18:09:12.76 SS.seek 0ml @18:09:19.85 PTV.dial :puck @18:09:19.86 PSV.dial PTV @18:09:20.61 PBV.dial :waste1 @18:09:21.06 PS.seek 1.5ml @18:09:22.23 Exhaust.select :close @18:09:22.34 SSV.dial CBV @18:09:22.38 CBV.dial SSV @18:09:23.17 Allowing 130s for 25.0ml of remaining 725.0ml @18:09:23.18 Intake.select :open @18:09:25.35 Intake @14.6psia, vacuum from 9.1 to 12.1psi @18:09:25.37 SS.reconfigure :inhaling 0.70ml/s @18:09:25.42 SS.seek 25ml @18:09:26.93 delay 3 seconds @18:09:29.95 PBV.dial between PSV and waste1 @18:09:30.35 PS.seek 0ml @18:09:46.50 delay 2:00 @18:10:02.65 Stroke completed -- waiting for pressure to equalize ... @18:10:02.66 SS.reconfigure :exhale @18:10:02.71 SS.hold @18:10:17.18 Intake.select :close @18:10:17.34 SSV.dial :exhaust @18:10:18.13 Exhaust.select :open @18:10:18.25 SS.seek 0ml @18:10:27.73 Exhaust.select :close @18:10:27.84 SSV.dial CBV @18:10:27.88 CBV.dial SSV @18:10:28.66 Allowing 130s for 25.0ml of remaining 700.0ml @18:10:28.68 Intake.select :open @18:10:30.85 Intake @14.8psia, vacuum from 9.3 to 12.3psi @18:10:30.87 SS.reconfigure :inhaling 0.70ml/s @18:10:30.92 SS.seek 25ml @18:10:58.56 Vacuum too high -- waiting up to 40 seconds @18:10:58.58 SS.reconfigure :exhale @18:10:58.63 SS.hold @18:11:03.62 Intake @13.5psia, vacuum from 8.0 to 11.0psi @18:11:03.64 SS.reconfigure :inhaling 0.62ml/s @18:11:03.69 SS.seek 25ml @18:11:14.83 Stroke completed -- waiting for pressure to equalize ... @18:11:14.85 SS.reconfigure :exhale @18:11:14.89 SS.hold @18:11:25.14 Intake.select :close @18:11:25.30 SSV.dial :exhaust @18:11:26.07 Exhaust.select :open @18:11:26.18 SS.seek 0ml @18:11:35.41 Exhaust.select :close @18:11:35.53 SSV.dial CBV @18:11:35.55 CBV.dial SSV @18:11:36.35 Allowing 130s for 25.0ml of remaining 675.0ml @18:11:36.36 Intake.select :open @18:11:38.52 Intake @14.5psia, vacuum from 9.0 to 12.0psi @18:11:38.54 SS.reconfigure :inhaling 0.62ml/s @18:11:38.59 SS.seek 25ml @18:11:46.52 recoverPSRreagent08 @18:11:46.54 PTV.dial :puck @18:11:46.55 PBV.dial :waste1 @18:11:46.92 delay 30 seconds @18:12:13.25 Vacuum too high -- waiting up to 40 seconds @18:12:13.27 SS.reconfigure :exhale @18:12:13.32 SS.hold @18:12:16.93 PBV.dial between PSV and waste1 @18:12:17.36 PSR.select :air @18:12:17.38 PSV.dial PSR @18:12:18.27 PS.reconfigure :fast @18:12:18.32 PS.seek 2.5ml @18:12:18.80 Intake @13.6psia, vacuum from 8.1 to 11.1psi @18:12:18.82 SS.reconfigure :inhaling 0.56ml/s @18:12:18.87 SS.seek 25ml @18:12:26.97 Stroke completed -- waiting for pressure to equalize ... @18:12:26.98 SS.reconfigure :exhale @18:12:27.03 SS.hold @18:12:36.10 Intake.select :close @18:12:36.26 SSV.dial :exhaust @18:12:37.02 Exhaust.select :open @18:12:37.14 SS.seek 0ml @18:12:41.84 PSV.dial PTV @18:12:42.59 delay 5 seconds @18:12:46.16 Exhaust.select :close @18:12:46.27 SSV.dial CBV @18:12:46.31 CBV.dial SSV @18:12:47.07 Allowing 130s for 25.0ml of remaining 650.0ml @18:12:47.09 Intake.select :open @18:12:47.61 PSV.dial PSR @18:12:48.35 delay 5 seconds @18:12:49.26 Intake @14.9psia, vacuum from 9.4 to 12.4psi @18:12:49.27 SS.reconfigure :inhaling 0.56ml/s @18:12:49.32 SS.seek 25ml @18:12:53.37 PSV.dial PTV @18:12:54.14 delay 5 seconds @18:12:59.16 PSV.dial :air @18:12:59.92 PS.seek 0ml @18:13:23.31 getPMLReagent08 @18:13:23.32 PRV.dial :air @18:13:23.76 delay 5 seconds @18:13:28.78 PTV.dial :PRVmixing @18:13:30.10 delay 5 seconds @18:13:34.96 Stroke completed -- waiting for pressure to equalize ... @18:13:34.97 SS.reconfigure :exhale @18:13:35.02 SS.hold @18:13:35.12 PRV.dial :ab2 @18:13:35.63 PTV.dial :PRVmixing @18:13:35.65 PSV.dial :mixloop @18:13:36.15 PS.reconfigure :default @18:13:36.20 PS.seek 0.3ml @18:13:40.41 delay 2 seconds @18:13:42.42 PRV.dial :air @18:13:42.96 PS.seek 0.55ml @18:13:46.30 addPMLReagent08 @18:13:46.32 PSV.dial :air @18:13:46.82 PS.seek 2ml @18:13:51.95 Intake.select :close @18:13:52.11 SSV.dial :exhaust @18:13:52.88 Exhaust.select :open @18:13:52.99 SS.seek 0ml @18:14:02.35 Exhaust.select :close @18:14:02.43 PSV.dial :PML @18:14:02.49 SSV.dial CBV @18:14:02.51 CBV.dial SSV @18:14:02.90 PTV.dial :mixing @18:14:03.35 Allowing 130s for 25.0ml of remaining 625.0ml @18:14:03.36 Intake.select :open @18:14:03.65 PBV.dial :waste1 @18:14:04.09 PS.seek 1.85ml @18:14:05.52 Intake @15.0psia, vacuum from 9.5 to 12.5psi @18:14:05.54 SS.reconfigure :inhaling 0.56ml/s @18:14:05.59 SS.seek 25ml @18:14:06.46 PBV.dial between PSV and waste1 @18:14:06.84 PS.seek 0ml @18:14:26.59 delay 2 seconds @18:14:28.61 delay 8:00 @18:14:35.59 Vacuum too high -- waiting up to 40 seconds @18:14:35.61 SS.reconfigure :exhale @18:14:35.66 SS.hold @18:14:41.09 Intake @14.1psia, vacuum from 8.6 to 11.6psi @18:14:41.11 SS.reconfigure :inhaling 0.51ml/s @18:14:41.16 SS.seek 25ml @18:14:58.86 Stroke completed -- waiting for pressure to equalize ... @18:14:58.87 SS.reconfigure :exhale @18:14:58.92 SS.hold @18:15:11.63 Intake.select :close @18:15:11.79 SSV.dial :exhaust @18:15:12.58 Exhaust.select :open @18:15:12.69 SS.seek 0ml @18:15:21.95 Exhaust.select :close @18:15:22.07 SSV.dial CBV @18:15:22.10 CBV.dial SSV @18:15:22.91 Allowing 130s for 25.0ml of remaining 600.0ml @18:15:22.92 Intake.select :open @18:15:25.09 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:15:25.10 SS.reconfigure :inhaling 0.51ml/s @18:15:25.15 SS.seek 25ml @18:16:06.09 Vacuum too high -- waiting up to 40 seconds @18:16:06.10 SS.reconfigure :exhale @18:16:06.16 SS.hold @18:16:13.75 Intake @14.5psia, vacuum from 9.0 to 12.0psi @18:16:13.77 SS.reconfigure :inhaling 0.46ml/s @18:16:14.28 SS.seek 25ml @18:16:25.58 Stroke completed -- waiting for pressure to equalize ... @18:16:25.59 SS.reconfigure :exhale @18:16:25.64 SS.hold @18:16:39.95 Intake.select :close @18:16:40.11 SSV.dial :exhaust @18:16:40.88 Exhaust.select :open @18:16:40.99 SS.seek 0ml @18:16:50.33 Exhaust.select :close @18:16:50.44 SSV.dial CBV @18:16:50.46 CBV.dial SSV @18:16:51.28 Allowing 130s for 25.0ml of remaining 575.0ml @18:16:51.29 Intake.select :open @18:16:53.93 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:16:53.94 SS.reconfigure :inhaling 0.46ml/s @18:16:53.99 SS.seek 25ml @18:17:30.52 Vacuum too high -- waiting up to 40 seconds @18:17:30.54 SS.reconfigure :exhale @18:17:30.59 SS.hold @18:17:37.22 Intake @14.7psia, vacuum from 9.2 to 12.2psi @18:17:37.24 SS.reconfigure :inhaling 0.42ml/s @18:17:37.29 SS.seek 25ml @18:17:58.28 Stroke completed -- waiting for pressure to equalize ... @18:17:58.29 SS.reconfigure :exhale @18:17:58.34 SS.hold @18:18:13.21 Intake.select :close @18:18:13.37 SSV.dial :exhaust @18:18:14.14 Exhaust.select :open @18:18:14.25 SS.seek 0ml @18:18:23.65 Exhaust.select :close @18:18:23.77 SSV.dial CBV @18:18:23.79 CBV.dial SSV @18:18:24.62 Allowing 130s for 25.0ml of remaining 550.0ml @18:18:24.64 Intake.select :open @18:18:26.81 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:18:26.82 SS.reconfigure :inhaling 0.42ml/s @18:18:26.87 SS.seek 25ml @18:19:27.40 Vacuum too high -- waiting up to 40 seconds @18:19:27.41 SS.reconfigure :exhale @18:19:27.47 SS.hold @18:19:38.77 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:19:38.79 SS.reconfigure :inhaling 0.38ml/s @18:19:38.84 SS.seek 25ml @18:19:40.10 Stroke completed -- waiting for pressure to equalize ... @18:19:40.12 SS.reconfigure :exhale @18:19:40.17 SS.hold @18:19:54.70 Intake.select :close @18:19:54.86 SSV.dial :exhaust @18:19:55.62 Exhaust.select :open @18:19:55.74 SS.seek 0ml @18:20:05.15 Exhaust.select :close @18:20:05.27 SSV.dial CBV @18:20:05.29 CBV.dial SSV @18:20:06.09 Allowing 130s for 25.0ml of remaining 525.0ml @18:20:06.10 Intake.select :open @18:20:08.27 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:20:08.29 SS.reconfigure :inhaling 0.38ml/s @18:20:08.34 SS.seek 25ml @18:21:15.36 Vacuum too high -- waiting up to 40 seconds @18:21:15.38 SS.reconfigure :exhale @18:21:15.43 SS.hold @18:21:25.21 Intake @14.8psia, vacuum from 9.3 to 12.3psi @18:21:25.23 SS.reconfigure :inhaling 0.33ml/s @18:21:25.28 SS.seek 25ml @18:21:27.43 Stroke completed -- waiting for pressure to equalize ... @18:21:27.44 SS.reconfigure :exhale @18:21:27.49 SS.hold @18:21:45.88 Intake.select :close @18:21:46.03 SSV.dial :exhaust @18:21:47.27 Exhaust.select :open @18:21:47.39 SS.seek 0ml @18:21:56.93 Exhaust.select :close @18:21:57.05 SSV.dial CBV @18:21:57.08 CBV.dial SSV @18:21:57.87 Allowing 130s for 25.0ml of remaining 500.0ml @18:21:57.88 Intake.select :open @18:22:00.05 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:22:00.06 SS.reconfigure :inhaling 0.33ml/s @18:22:00.11 SS.seek 25ml @18:22:28.63 recoverPRVreagent08 @18:22:28.64 PTV.dial :mixing @18:22:28.82 PBV.dial :waste1 @18:22:29.21 delay 30 seconds @18:22:59.23 PBV.dial between PSV and waste1 @18:22:59.63 PSV.dial :air @18:23:00.10 PS.reconfigure :fast @18:23:00.15 PS.seek 2.5ml @18:23:18.76 Stroke completed -- waiting for pressure to equalize ... @18:23:18.77 SS.reconfigure :exhale @18:23:18.82 SS.hold @18:23:23.89 PSV.dial :mixloop @18:23:24.36 delay 2 seconds @18:23:26.38 PSV.dial :air @18:23:26.85 delay 2 seconds @18:23:28.86 PSV.dial :mixloop @18:23:29.33 delay 2 seconds @18:23:31.35 PSV.dial :air @18:23:31.82 PS.seek 0ml @18:23:45.41 Intake.select :close @18:23:45.57 SSV.dial :exhaust @18:23:46.34 Exhaust.select :open @18:23:46.46 SS.seek 0ml @18:23:55.24 washCycleDA #1 of 3 @18:23:55.25 PSR.select :air @18:23:55.27 PSV.dial PSR @18:23:55.85 Exhaust.select :close @18:23:55.96 SSV.dial CBV @18:23:56.00 CBV.dial SSV @18:23:56.59 Pulling 0.5ml of PSR.wash @18:23:56.61 PSR.select :wash @18:23:56.72 PS.reconfigure :default @18:23:56.79 Allowing 130s for 25.0ml of remaining 475.0ml @18:23:56.81 PS.seek 0.5ml @18:23:56.83 Intake.select :open @18:23:59.05 Intake @15.7psia, vacuum from 10.0 to 13.0psi @18:23:59.07 SS.reconfigure :inhaling 0.33ml/s @18:23:59.11 SS.seek 25ml @18:24:02.74 delay 5 seconds @18:24:07.76 PSR.select :air @18:24:07.88 PS.seek 2ml @18:24:24.05 PTV.dial :puck @18:24:24.81 PSV.dial PTV @18:24:25.56 PBV.dial :waste1 @18:24:26.02 PS.seek 1.5ml @18:24:31.95 delay 3 seconds @18:24:34.97 PBV.dial between PSV and waste1 @18:24:35.83 PS.seek 0ml @18:24:51.99 delay 2:00 @18:25:13.30 Vacuum too high -- waiting up to 40 seconds @18:25:13.31 SS.reconfigure :exhale @18:25:13.37 SS.hold @18:25:22.04 Intake @15.0psia, vacuum from 9.5 to 12.5psi @18:25:22.06 SS.reconfigure :inhaling 0.30ml/s @18:25:22.11 SS.seek 25ml @18:25:27.61 Stroke completed -- waiting for pressure to equalize ... @18:25:27.63 SS.reconfigure :exhale @18:25:27.67 SS.hold @18:25:48.58 Intake.select :close @18:25:48.74 SSV.dial :exhaust @18:25:49.53 Exhaust.select :open @18:25:49.64 SS.seek 0ml @18:25:59.22 Exhaust.select :close @18:25:59.34 SSV.dial CBV @18:25:59.36 CBV.dial SSV @18:26:00.16 Allowing 130s for 25.0ml of remaining 450.0ml @18:26:00.17 Intake.select :open @18:26:02.34 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:26:02.36 SS.reconfigure :inhaling 0.30ml/s @18:26:02.41 SS.seek 25ml @18:26:52.01 recoverPSRreagent08 @18:26:52.03 PTV.dial :puck @18:26:52.09 PBV.dial :waste1 @18:26:52.49 delay 30 seconds @18:27:22.51 PBV.dial between PSV and waste1 @18:27:22.91 PSR.select :air @18:27:22.92 PSV.dial PSR @18:27:23.68 PS.reconfigure :fast @18:27:23.73 PS.seek 2.5ml @18:27:25.36 Vacuum too high -- waiting up to 40 seconds @18:27:25.37 SS.reconfigure :exhale @18:27:25.43 SS.hold @18:27:35.81 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:27:35.82 SS.reconfigure :inhaling 0.28ml/s @18:27:35.87 SS.seek 25ml @18:27:40.78 Stroke completed -- waiting for pressure to equalize ... @18:27:40.79 SS.reconfigure :exhale @18:27:40.84 SS.hold @18:27:47.20 PSV.dial PTV @18:27:47.95 delay 5 seconds @18:27:52.97 PSV.dial PSR @18:27:53.72 delay 5 seconds @18:27:58.74 PSV.dial PTV @18:27:59.48 delay 5 seconds @18:28:03.41 Intake.select :close @18:28:03.60 SSV.dial :exhaust @18:28:04.42 Exhaust.select :open @18:28:04.50 PSV.dial :air @18:28:04.55 SS.seek 0ml @18:28:05.27 PS.seek 0ml @18:28:14.08 Exhaust.select :close @18:28:14.19 SSV.dial CBV @18:28:14.22 CBV.dial SSV @18:28:15.02 Allowing 130s for 25.0ml of remaining 425.0ml @18:28:15.03 Intake.select :open @18:28:17.20 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:28:17.22 SS.reconfigure :inhaling 0.28ml/s @18:28:17.27 SS.seek 25ml @18:28:28.86 washCycleDA #2 of 3 @18:28:28.87 PSR.select :air @18:28:28.89 PSV.dial PSR @18:28:30.23 Pulling 0.5ml of PSR.wash @18:28:30.24 PSR.select :wash @18:28:30.84 PS.reconfigure :default @18:28:30.89 PS.seek 0.5ml @18:28:36.76 delay 5 seconds @18:28:41.78 PSR.select :air @18:28:41.91 PS.seek 2ml @18:28:58.01 PTV.dial :puck @18:28:58.09 PSV.dial PTV @18:28:58.84 PBV.dial :waste1 @18:28:59.29 PS.seek 1.5ml @18:29:05.20 delay 3 seconds @18:29:08.22 PBV.dial between PSV and waste1 @18:29:08.61 PS.seek 0ml @18:29:24.73 delay 2:00 @18:29:54.55 Stroke completed -- waiting for pressure to equalize ... @18:29:54.56 SS.reconfigure :exhale @18:29:54.61 SS.hold @18:30:25.04 SS.reconfigure :default @18:30:25.09 SS.hold @18:30:25.17 Intake.select :close @18:30:25.33 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 600.0ml @18:30:25.34 SSV.dial :exhaust @18:30:26.11 Exhaust.select :open @18:30:26.22 SS.reconfigure :exhale @18:30:26.28 SS.seek 0ml @18:30:35.95 Exhaust.select :close @18:30:36.07 Sampled 600.0ml @18:30:36.08 Drying puck before fixing for 4:00 @18:30:36.09 CBV.dial :waste2b @18:30:36.86 delay 1:00 @18:31:24.76 recoverPSRreagent08 @18:31:24.77 PTV.dial :puck @18:31:24.84 PBV.dial :waste1 @18:31:25.23 delay 30 seconds @18:31:36.88 CSV.dial :air @18:31:37.35 CS.reconfigure :fast @18:31:37.40 CS.seek 10ml @18:31:55.25 PBV.dial between PSV and waste1 @18:31:55.64 PSR.select :air @18:31:55.66 PSV.dial PSR @18:31:56.41 PS.reconfigure :fast @18:31:56.46 PS.seek 2.5ml @18:32:01.27 Pumping wcr puck from tube 3 up to 35psi... @18:32:01.29 CBV.dial SSV @18:32:02.55 CBV.dial between CSV and waste2b @18:32:03.52 CSV.dial between air and CTV @18:32:03.98 CS.seek 6.629ml @18:32:12.52 CTV.dial CSV,avoiding: :air @18:32:12.55 CSV.dial CTV @18:32:13.38 CS.reconfigure :pressurize @18:32:13.43 CS.seek 0ml @18:32:20.04 PSV.dial PTV @18:32:20.79 delay 5 seconds @18:32:25.81 PSV.dial PSR @18:32:26.56 delay 5 seconds @18:32:27.82 CBV.dial :waste2b @18:32:28.16 CS.reconfigure :default @18:32:28.21 CS.seek 0ml @18:32:31.58 PSV.dial PTV @18:32:32.34 delay 5 seconds @18:32:32.49 delay 1:00 @18:32:37.36 PSV.dial :air @18:32:38.13 PS.seek 0ml @18:33:01.45 washCycleDA #3 of 3 @18:33:01.46 PSR.select :air @18:33:01.48 PSV.dial PSR @18:33:02.81 Pulling 0.5ml of PSR.wash @18:33:02.82 PSR.select :wash @18:33:02.94 PS.reconfigure :default @18:33:02.99 PS.seek 0.5ml @18:33:08.95 delay 5 seconds @18:33:13.97 PSR.select :air @18:33:14.09 PS.seek 2ml @18:33:30.20 PTV.dial :puck @18:33:30.28 PSV.dial PTV @18:33:31.01 PBV.dial :waste1 @18:33:31.46 PS.seek 1.5ml @18:33:32.51 CTV.dial :air,avoiding: :intake @18:33:33.87 Fix #1 of 1 (with RNAlater) for 4:00 @18:33:33.88 CSV.dial CSR @18:33:34.31 Pulling 2ml of CSR.RNAlater @18:33:34.32 CSR.select :RNAlater @18:33:34.44 CS.seek 2ml @18:33:37.35 delay 3 seconds @18:33:40.33 delay 5 seconds @18:33:40.37 PBV.dial between PSV and waste1 @18:33:40.77 PS.seek 0ml @18:33:45.35 CSR.select :air @18:33:45.46 CS.reconfigure :fast @18:33:45.51 CS.seek 10ml @18:33:57.08 delay 2:00 @18:34:04.83 Pumping wcr puck from tube 3 up to 35psi... @18:34:04.85 CBV.dial SSV @18:34:06.10 CBV.dial between CSV and waste2b @18:34:07.07 CSV.dial between air and CTV @18:34:07.81 CS.seek 6.563ml @18:34:16.58 CTV.dial CSV,avoiding: :air @18:34:16.61 CSV.dial CTV @18:34:18.46 CS.reconfigure :pressurize @18:34:18.55 CS.seek 0ml @18:34:32.47 delay 4:00 @18:35:57.09 recoverPSRreagent08 @18:35:57.11 PTV.dial :puck @18:35:57.18 PBV.dial :waste1 @18:35:57.57 delay 30 seconds @18:36:27.60 PBV.dial between PSV and waste1 @18:36:27.98 PSR.select :air @18:36:28.00 PSV.dial PSR @18:36:28.75 PS.reconfigure :fast @18:36:28.80 PS.seek 2.5ml @18:36:52.38 PSV.dial PTV @18:36:53.13 delay 5 seconds @18:36:58.16 PSV.dial PSR @18:36:58.90 delay 5 seconds @18:37:03.92 PSV.dial PTV @18:37:04.67 delay 5 seconds @18:37:09.69 PSV.dial :air @18:37:10.47 PS.seek 0ml @18:37:33.63 Gate.power :camera,:ON @18:37:33.65 Mixing DA Substrates @18:37:33.66 PSR.select :air @18:37:33.67 PSV.dial PSR @18:37:35.05 Pulling 0.25ml of PSR.sub1 @18:37:35.10 PSR.select :sub1 @18:37:35.28 PS.reconfigure :default @18:37:35.43 PS.seek 0.25ml @18:37:38.98 delay 5 seconds @18:37:44.00 Pulling 0.25ml of PSR.sub2 @18:37:44.01 PSR.select :sub2 @18:37:44.13 PS.seek 0.5ml @18:37:47.54 delay 5 seconds @18:37:52.56 PSR.select :air @18:37:52.67 PS.seek 2ml @18:38:08.82 PTV.dial :puck @18:38:08.90 PSV.dial PTV @18:38:09.66 PBV.dial :waste1 @18:38:10.10 PS.seek 1.5ml @18:38:16.07 delay 3 seconds @18:38:19.09 PBV.dial between PSV and waste1 @18:38:19.47 PS.seek 0ml @18:38:32.50 CBV.dial :waste2b @18:38:32.84 CS.reconfigure :default @18:38:32.90 CS.seek 0ml @18:38:35.60 delay 10 seconds @18:38:37.04 delay 1:00 @18:38:45.63 recoverPSRreagent08 @18:38:45.64 PTV.dial :puck @18:38:45.71 PBV.dial :waste1 @18:38:46.09 delay 30 seconds @18:39:16.11 PBV.dial between PSV and waste1 @18:39:16.49 PSR.select :air @18:39:16.51 PSV.dial PSR @18:39:17.39 PS.reconfigure :fast @18:39:17.44 PS.seek 2.5ml @18:39:37.07 CTV.dial :air,avoiding: :intake @18:39:41.07 PSV.dial PTV @18:39:41.82 delay 5 seconds @18:39:46.84 PSV.dial PSR @18:39:47.71 delay 5 seconds @18:39:52.73 PSV.dial PTV @18:39:53.91 delay 5 seconds @18:39:58.99 PSV.dial :air @18:39:59.78 PS.seek 0ml @18:40:23.29 Additional air clear @18:40:23.30 PSR.select :air @18:40:23.32 PSV.dial PSR @18:40:25.40 Pulling 2ml of PSR.air @18:40:25.41 PSR.select :air @18:40:25.43 PS.reconfigure :default @18:40:25.48 PS.seek 2ml @18:40:46.72 delay 5 seconds @18:40:51.75 PTV.dial :puck @18:40:51.83 PSV.dial PTV @18:40:52.58 PBV.dial :waste1 @18:40:53.02 PS.seek 1.5ml @18:40:59.01 delay 3 seconds @18:41:02.03 PBV.dial between PSV and waste1 @18:41:02.40 delay 1 second @18:41:03.42 PSV.dial :waste1 @18:41:04.60 PS.seek 0ml @18:41:20.87 Processing Puck Heater at 28C before imaging @18:41:20.88 PH.coast @18:41:20.99 Imaging da puck in Processing Clamp while storing it to tube 2 @18:41:21.17 SC.reconfigure :tight @18:41:21.22 SC.seek 2 @18:41:24.87 SC.configure :limp @18:41:25.09 Elbow.reconfigure :tight @18:41:25.14 Elbow.seek 2 @18:41:26.89 Forearm.reconfigure :tight @18:41:26.94 Forearm.seek 2 + 800 counts @18:41:27.95 SE.reconfigure :pick @18:41:28.01 SE.seek :beamBreak @18:41:30.02 SE.configure :default @18:41:30.18 Elbow.reconfigure :limp @18:41:30.23 Forearm.reconfigure :loose @18:41:30.27 Forearm.seek 2 @18:41:30.64 Elbow.hold @18:41:30.71 Elbow.reconfigure :tight @18:41:30.80 SE.seek 11.09 Pucks below Top Plate @18:41:31.28 Forearm.force -20 @18:41:31.32 Forearm.seek 2 + 311 counts @18:41:31.66 Forearm.reconfigure :tight @18:41:32.15 Forearm.seek 2 + 800 counts @18:41:32.55 SE.seek 12.09 Pucks below Top Plate @18:41:33.18 Forearm.reconfigure :loose @18:41:33.23 Forearm.seek :retract @18:41:34.14 Elbow.seek PC @18:41:36.68 Elbow.reconfigure :loose @18:41:36.78 Forearm.seek PC @18:41:37.86 Hand.close @18:41:38.61 PC.seek :open @18:41:43.19 Forearm.seek :retract @18:41:44.25 Elbow.reconfigure :tight @18:41:44.30 Elbow.seek 2 @18:41:46.93 Forearm.reconfigure :tight @18:41:46.98 Forearm.seek 2 + 800 counts @18:41:48.01 Hand.open @18:41:48.79 SE.seek 11.85 Pucks below Top Plate @18:41:49.03 SE.reconfigure :seal @18:41:49.08 SE.seek 11.19 Pucks below Top Plate @18:41:49.61 Lights, camera, ACTION! @18:41:49.65 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da25oct2218h875ml.tif @18:42:09.14 Waiting >20 seconds for da to relinquish FlushPuck @18:42:13.16 SX HX9/16 (USB:08): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20.204 seconds /var/log/esp/da25oct2218h875ml.tif: TIFF Upload Complete @18:42:13.19 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da25oct2218h875ml15s.tif @18:42:28.80 SX HX9/16 (USB:08): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da25oct2218h875ml15s.tif: TIFF Upload Complete @18:42:28.83 PuckCamera: snap -bin=2,2 21.0 /var/log/esp/midres/da25oct2218h875ml21s.tif @18:42:50.44 SX HX9/16 (USB:08): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21 seconds /var/log/esp/midres/da25oct2218h875ml21s.tif: TIFF Upload Complete @18:42:50.46 Gate.power :camera,:OFF @18:42:50.48 DAfans.email "20.204 sec auto-exposure",Subject: "da25oct2218h875ml images!" @18:42:50.90 Elbow.seek 2 @18:42:51.13 Forearm.seek 2 + 800 counts @18:42:51.22 SE.reconfigure :gently @18:42:51.27 SE.seek 11.91 Pucks below Top Plate @18:42:51.98 Elbow.reconfigure :limp @18:42:52.05 Forearm.reconfigure :loose @18:42:52.10 Forearm.seek 2 @18:42:53.05 Elbow.hold @18:42:53.13 Elbow.reconfigure :tight @18:42:53.19 SE.reconfigure :default @18:42:53.24 SE.seek 12.09 Pucks below Top Plate @18:42:53.68 Forearm.force -20 @18:42:53.72 Forearm.seek 2 + 306 counts @18:42:54.34 Forearm.reconfigure :tight @18:42:54.39 Forearm.seek 2 + 800 counts @18:42:54.71 SE.seek :down @18:42:55.99 Forearm.reconfigure :loose @18:42:56.08 Forearm.seek :retract @18:42:56.28 SE.stop @18:42:57.16 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:42:57.77 Elbow.seek FlushPuck::Garage @18:42:59.68 Elbow.reconfigure :loose @18:42:59.92 Forearm.seek :garage @18:43:01.03 Hand.close @18:43:02.16 Forearm.seek :retract @18:43:03.22 Elbow.reconfigure :tight @18:43:03.32 Elbow.seek PC @18:43:04.77 Elbow.reconfigure :loose @18:43:04.97 Forearm.reconfigure :tight @18:43:05.09 Forearm.seek PC @18:43:06.62 PC.seek :closed @18:43:11.51 Clamp::VelocityError in psFlush -- Processing Clamp did not close properly @18:43:11.55 Retry #1 of 2 @18:43:12.00 PC.seek :open @18:43:16.63 PC.seek :closed @18:43:17.23 Waiting >20 seconds for psFlush to relinquish Arm @18:43:21.19 Processing Clamp onSpring/offSpring for 12/26 samples, on/off torque 1.56 @18:43:21.31 Hand.open @18:43:22.35 Forearm.reconfigure :loose @18:43:22.47 Forearm.seek :retract @18:43:23.47 PSR.select :air @18:43:23.52 Storing wcr puck in Collection Clamp to tube 2 @18:43:23.57 PSV.dial PSR @18:43:24.06 SC.reconfigure :tight @18:43:24.16 SC.seek 2 @18:43:24.32 Pulling 0.5ml of PSR.flush @18:43:24.34 PSR.select :flush @18:43:24.45 SC.configure :limp @18:43:24.78 PS.seek 0.5ml @18:43:25.04 Elbow.reconfigure :tight @18:43:25.14 Elbow.seek 2 @18:43:27.40 upsync @18:43:27.79 Forearm.reconfigure :tight @18:43:27.84 Forearm.seek 2 + 800 counts @18:43:28.92 SE.reconfigure :pick @18:43:28.98 SE.seek :beamBreak @18:43:30.83 SE.configure :default @18:43:30.90 delay 5 seconds @18:43:31.51 Elbow.reconfigure :limp @18:43:31.56 Forearm.reconfigure :loose @18:43:31.61 Forearm.seek 2 @18:43:31.97 Elbow.hold @18:43:32.04 Elbow.reconfigure :tight @18:43:32.15 SE.seek 12.09 Pucks below Top Plate @18:43:32.70 Forearm.force -20 @18:43:32.74 Forearm.seek 2 + 312 counts @18:43:33.03 Forearm.reconfigure :tight @18:43:33.08 Forearm.seek 2 + 800 counts @18:43:33.47 SE.seek 13.09 Pucks below Top Plate @18:43:34.01 Forearm.reconfigure :loose @18:43:34.06 Forearm.seek :retract @18:43:35.03 Elbow.seek CC @18:43:35.93 PSR.select :air @18:43:36.04 PS.seek 2.5ml @18:43:36.67 Elbow.reconfigure :loose @18:43:36.77 Forearm.seek CC @18:43:37.77 Hand.close @18:43:38.49 CC.seek :open @18:43:42.71 Forearm.seek :retract @18:43:43.72 Elbow.reconfigure :tight @18:43:43.77 Elbow.seek 2 @18:43:45.47 Elbow.reconfigure :limp @18:43:45.53 Forearm.seek 2 @18:43:46.69 Elbow.hold @18:43:46.76 Elbow.reconfigure :tight @18:43:47.25 Hand.open @18:43:48.10 Forearm.force -20 @18:43:48.15 Forearm.seek 2 + 315 counts @18:43:48.45 Forearm.reconfigure :tight @18:43:48.50 Forearm.seek 2 + 800 counts @18:43:48.81 SE.seek :down @18:43:49.96 Forearm.reconfigure :loose @18:43:50.01 Forearm.seek :retract @18:43:50.26 SE.stop @18:43:51.06
Starting finalPurge @18:43:57.22 delay 1 second @18:43:58.24 PRV.dial :waste2,avoiding: :ab2 @18:44:00.11 PTV.dial :PRVmixing @18:44:01.41 PSV.dial :mixloop @18:44:02.48 PS.seek :empty @18:44:10.31
Waiting >20 seconds for psFlush to relinquish FlushPuck @18:44:28.84 PSR.select :air @18:44:28.86 PSV.dial PSR @18:44:29.91 Pulling 0.5ml of PSR.flush @18:44:29.92 PSR.select :flush @18:44:30.04 PS.seek 0.5ml @18:44:35.94 delay 5 seconds @18:44:40.96 PSR.select :air @18:44:41.08 PS.seek 2.5ml @18:45:02.38 delay 1 second @18:45:03.40 PBV.dial :waste1 @18:45:03.77 PTV.dial :mixing @18:45:04.50 PSV.dial :mixloop @18:45:05.55 PS.seek :empty @18:45:31.94 PSV.dial PSR @18:45:32.99 PSR.select :flush @18:45:33.11 PS.seek 1ml @18:45:44.25 PSR.select :air @18:45:44.37 PS.seek 2.5ml @18:46:00.53 PSV.dial PTV @18:46:01.30 PTV.dial :puck @18:46:02.03 PBV.dial :waste1 @18:46:02.12 PS.seek 0ml @18:46:28.50 PSV.dial PSR @18:46:29.25 PSR.select :flush @18:46:29.82 PS.seek 0.5ml @18:46:35.69 PSR.select :air @18:46:35.81 PS.seek 2.5ml @18:46:57.06 PSV.dial PTV @18:46:57.81 PBV.dial PSV @18:46:58.40 PS.seek 0ml @18:47:24.75 PSV.dial PBV @18:47:25.21 PS.seek 2.5ml @18:47:51.66 PSV.dial :waste1 @18:47:52.40 PS.seek 0ml @18:48:18.78 PSV.dial PSR @18:48:19.25 PSR.select :air @18:48:19.28 PS.seek 2.5ml @18:48:45.65 PSV.dial PTV @18:48:46.39 PBV.dial PSV @18:48:46.47 PS.seek 0ml @18:49:12.81 PSV.dial PBV @18:49:13.40 PS.seek 2.5ml @18:49:39.78 PSV.dial :waste1 @18:49:40.54 PS.seek 0ml @18:50:06.84 PSV.dial PSR @18:50:07.31 PSR.select :flush @18:50:07.43 PS.seek 1ml @18:50:18.43 PSR.select :air @18:50:18.55 PS.seek 2.5ml @18:50:34.72 PSV.dial PTV @18:50:35.45 PTV.dial :puck @18:50:35.53 PBV.dial :waste1 @18:50:36.11 PS.seek 0ml @18:51:02.44 PSV.dial PSR @18:51:03.18 PSR.select :flush @18:51:03.30 PS.seek 0.5ml @18:51:09.18 PSR.select :air @18:51:09.30 PS.seek 2.5ml @18:51:30.53 PSV.dial PTV @18:51:31.28 PBV.dial PSV @18:51:31.86 PS.seek 0ml @18:51:58.22 PSV.dial PBV @18:51:58.67 PS.seek 2.5ml @18:52:25.06 PSV.dial :waste1 @18:52:25.80 PS.seek 0ml @18:52:52.40 PSV.dial PSR @18:52:52.87 PSR.select :air @18:52:53.34 PS.seek 2.5ml @18:53:19.71 PSV.dial PTV @18:53:20.45 PBV.dial PSV @18:53:20.53 PS.seek 0ml @18:53:46.93 PSV.dial PBV @18:53:47.52 PS.seek 2.5ml @18:54:13.96 PSV.dial :waste1 @18:54:14.74 PS.seek 0ml @18:54:41.10
Loading FlushPuck into Collection Clamp from Processing Clamp @18:54:41.29 Elbow.seek PC @18:54:43.79 Elbow.reconfigure :loose @18:54:43.89 Forearm.seek PC @18:54:44.95 Hand.close @18:54:45.72 PC.seek :open @18:54:50.24 Forearm.seek :retract @18:54:51.30 Elbow.reconfigure :tight @18:54:51.35 Elbow.seek CC @18:54:54.92 Elbow.reconfigure :loose @18:54:55.02 Forearm.reconfigure :tight @18:54:55.06 Forearm.seek CC @18:54:56.36 CC.seek :closed @18:55:00.37 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.04 @18:55:00.44 Hand.open @18:55:01.29 Forearm.reconfigure :loose @18:55:01.34 Forearm.seek :retract @18:55:02.49 Flushing the Collection Valve::Manifold @18:55:02.61 CSV.dial CSR @18:55:03.04 Pulling 5ml of CSR.flush @18:55:03.47 CSR.select :flush @18:55:03.64 CS.seek 5ml @18:55:17.31 delay 5 seconds @18:55:22.33 CSR.select :air @18:55:22.50 CS.reconfigure :fast @18:55:22.55 CS.seek 7.5ml @18:55:29.18 CSV.dial :waste @18:55:30.45 CS.reconfigure :default @18:55:30.50 CS.seek 0ml @18:55:50.44 Flushing lower manifold's waste1 port @18:55:50.56 CSV.dial :flush @18:55:51.00 CS.reconfigure :fast @18:55:51.05 CS.seek 5ml @18:56:03.37 delay 5 seconds @18:56:08.39 CSV.dial :air @18:56:08.89 CS.seek 7ml @18:56:14.37 CSV.dial CTV @18:56:14.40
CTV.dial CSV @18:56:15.82 CBV.dial :waste1 @18:56:16.60 CS.seek 0ml @18:56:33.31 Flushing lower manifold's waste2 port @18:56:33.43 CSV.dial :flush @18:56:34.17 CS.seek 5ml @18:56:46.53 delay 5 seconds @18:56:51.55 CSV.dial :air @18:56:52.06 CS.seek 7ml @18:56:57.56 CSV.dial CTV @18:56:57.59
CTV.dial CSV @18:56:58.04 CBV.dial :waste2 @18:56:59.10 CS.seek 0ml @18:57:15.87 Flushing lower manifold's waste2b port @18:57:15.99 CSV.dial :flush @18:57:16.74 CS.seek 5ml @18:57:29.00 delay 5 seconds @18:57:34.01 CSV.dial :air @18:57:34.53 CS.seek 7ml @18:57:39.97 CSV.dial CTV @18:57:39.99
CTV.dial CSV @18:57:40.49 CBV.dial :waste2b @18:57:41.52 CS.seek 0ml @18:57:58.21 Disinfecting external Intake with kill @18:57:58.34 CSV.dial CSR @18:57:58.78 Pulling 7.999ml of CSR.kill @18:57:58.79 CSR.select :kill @18:57:58.90 CS.seek 8ml @18:58:18.50 delay 5 seconds @18:58:23.52 CSR.select :air @18:58:23.64 CS.seek 10ml @18:58:29.09 CSV.dial CBV @18:58:29.12
CBV.dial CSV @18:58:30.17 CTV.dial :intake @18:58:30.93 Intake.select :open @18:58:31.04 CS.seek 2ml @18:58:50.02 Intake.select :close @18:58:50.13 CTV.dial :air @18:58:50.89 CS.reconfigure :default @18:58:50.94 CS.seek 0ml @18:58:56.90 CSV.dial CSR @18:58:57.92 Pulling 5ml of CSR.flush CSR.select :flush @18:58:58.04 CS.seek 5ml @18:59:11.74 delay 5 seconds @18:59:16.76 CSR.select :air @18:59:16.88 CS.seek 10ml @18:59:30.80 CSV.dial CBV @18:59:31.83 CS.seek 0ml @18:59:58.31 Garaging FlushPuck in Collection Clamp @18:59:58.38 Elbow.reconfigure :tight @18:59:58.43 Elbow.seek CC @18:59:58.71 Elbow.reconfigure :loose @18:59:58.81 Forearm.seek CC @18:59:59.75 Hand.close @19:00:00.47 CC.seek :open @19:00:04.55 Forearm.seek :retract @19:00:05.53 Elbow.reconfigure :tight @19:00:05.58 Elbow.seek :garage @19:00:08.37 Elbow.reconfigure :loose @19:00:08.47 Forearm.reconfigure :tight @19:00:08.51 Forearm.seek :garage @19:00:09.53 Hand.open @19:00:10.28 Forearm.reconfigure :loose @19:00:10.32 Forearm.seek :retract @19:00:11.34 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 16:27:14 @19:00:11.40 Asleep after completing DA_WCR_102225 assays @19:00:11.41 GoodNews.email "{1 => 22,\n 2 => 14,\n 3 => 8,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@18:56:42, 40% flow, 21.0C, 22% humidity, 14.7psia, 13.71V, 0.365A avg, 181.801Ah, 5.01W\nSampled 600.0ml",Subject: "Asleep after completing DA_WCR_102225 assays" @19:00:15.70 upsync + ESPwhich DA_WCR_102925.rb + fn=DA_WCR_102925.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_102925.rb + [ -f /home/esp/esp2/mission/DA_WCR_102925.rb ] + realpath /home/esp/esp2/mission/DA_WCR_102925.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_102925.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_102925.rb @16:23:08.80UTC29-Oct-25
ESP2 v3.42 I2C Gateway v3.20 for core bus @16:23:11.44 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @16:23:15.11 Polling Can every 10:00 @16:23:15.17 Cleanly exited at 15:29:38UTC29-Oct-25 @16:23:15.22 Loading /home/esp/esp2local/mission/DA_WCR_102925.rb mission @16:23:15.27 GoodNews.email "Starting DA_WCR_102925 w/pucks in tube 3\n{1 => 22,\n 2 => 14,\n 3 => 8,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@16:23:15, 41% flow, 19.0C, 23% humidity, 14.6psia, 13.69V, 0.359A avg, 217.578Ah, 4.92W",Subject: "DA_WCR_102925 begun" @16:23:15.31
Preparing to sleep until + 00:00:05 @16:23:15.35 Asleep til + 00:00:05 @16:23:15.36 Continuing at 16:23 @16:23:15.66 Gate.power :core,:OFF @16:23:15.67 Gate.power :raw,:OFF @16:23:15.68 Shutdown @16:23:17.83 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_102925.rb:14\n{1 => 22,\n 2 => 14,\n 3 => 8,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@16:23:15, 41% flow, 19.0C, 23% humidity, 14.6psia, 13.69V, 0.359A avg, 217.578Ah, 4.92W",Subject: "Asleep until 16:23" @16:23:19.24 upsync @16:23:20.26
Powering core bus @16:23:21.73 upsync @16:23:25.91
Gate.power :raw,:ON @16:23:26.80 CS.configure :default @16:23:26.85 PS.configure :default @16:23:26.90 SS.configure :default @16:23:26.95 CC.configure :default @16:23:27.00 PC.configure :default @16:23:27.07 Forearm.configure :loose @16:23:27.12 Elbow.configure :loose @16:23:27.19 SC.configure :loose @16:23:27.24 SE.configure :default @16:23:27.29 CH.configure :default @16:23:27.33 PH.configure :default @16:23:27.37 All dwarf controllers running firmware version 3.85 @16:23:27.48 Valve.reset @16:23:27.70 Intake.select :close @16:23:27.73 Exhaust.select :close @16:23:27.77 CTV.dial :air @16:23:27.90 PSV.dial :air @16:23:27.95 CSV.dial :waste1 @16:23:28.06
Forearm.home.seek :retract @16:23:28.42 SSV.dial :spit,avoiding: :exhaust @16:23:28.81 Hand.close @16:23:28.85
Elbow.reconfigure :loose @16:23:28.93 Elbow.jog 1500 @16:23:29.02 PS.home.seek 0ml @16:23:29.30 CS.reconfigure :fast @16:23:29.34 SS.home.seek 0ml @16:23:29.42 CS.home.seek 0ml @16:23:29.50
Elbow.reconfigure :loose @16:23:29.56 Elbow.home.seek :clear @16:23:32.76 SE.home.seek :down @16:23:33.46 SC.reconfigure :tight @16:23:33.51 SC.home.seek :initial @16:23:38.21 PS.stop @16:23:46.22
SC.configure :limp @16:23:46.28 Forearm.seek :retract @16:23:46.34 Elbow.seek :hammer @16:23:47.43 Forearm.reconfigure :tight @16:23:47.48 Forearm.seek retracted @16:23:48.06 CC.home.seek :open @16:23:48.13 PC.home.seek :open @16:23:51.31
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @16:23:51.33 Notice.email "At 16:23:51 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_102925.rb:14\n{1 => 22,\n 2 => 14,\n 3 => 8,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@16:23:51, 41% flow, 19.1C, 23% humidity, 14.6psia, 13.68V, 0.309A avg, 217.582Ah, 4.23W",Subject: "Started DA_WCR_102925 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @16:23:51.35
Starting initialPurge @16:23:51.38 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:23:51.44 Hand.open @16:23:52.35 Elbow.reconfigure :tight @16:23:52.41 Elbow.seek FlushPuck::Garage @16:23:54.31 Elbow.reconfigure :loose @16:23:54.43 Forearm.reconfigure :loose @16:23:54.49 Forearm.seek :garage @16:23:55.44 upsync @16:23:55.54
Hand.close @16:23:56.46 Forearm.seek :retract @16:23:57.53 Elbow.reconfigure :tight @16:23:57.58 Elbow.seek CC @16:24:00.41 Elbow.reconfigure :loose @16:24:00.52 Forearm.reconfigure :tight @16:24:00.57 Forearm.seek CC @16:24:01.90 CC.seek :closed @16:24:05.87 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.94 @16:24:05.93 Hand.open @16:24:06.77 Forearm.reconfigure :loose @16:24:06.82 Forearm.seek :retract @16:24:07.94 Flushing the Collection Valve::Manifold @16:24:08.06 CSV.dial CSR @16:24:09.46 Pulling 4.999ml of CSR.flush @16:24:09.47 CSR.select :flush @16:24:09.59 CS.reconfigure :default @16:24:09.64 CS.seek 5ml @16:24:23.73 delay 5 seconds @16:24:28.75 CSR.select :air @16:24:28.92 CS.reconfigure :fast @16:24:28.97 CS.seek 7.5ml @16:24:35.64 CSV.dial :waste @16:24:36.92 CS.reconfigure :default @16:24:36.97 CS.seek 0ml @16:24:57.04 Purging external Intake @16:24:57.16 CSV.dial :flush @16:24:57.63 CS.reconfigure :fast @16:24:57.68 CS.seek 10ml @16:25:21.76 delay 5 seconds @16:25:26.78 CSV.dial CBV @16:25:26.83
CBV.dial CSV @16:25:27.54 CTV.dial :intake @16:25:28.32 Intake.select :open @16:25:28.44 CS.seek 0ml @16:25:52.20 Intake.select :close @16:25:52.31 CTV.dial :air @16:25:53.09 Flushing upper manifold @16:25:53.21 CSV.dial :flush @16:25:53.95 CS.seek 5ml @16:26:06.39 delay 5 seconds @16:26:11.40 CSV.dial :air @16:26:11.92 CS.seek 7ml @16:26:17.32 CSV.dial CBV @16:26:17.35
CBV.dial CSV @16:26:18.38 CTV.dial :air @16:26:18.40 CS.seek 0ml @16:26:35.32 delay 7 seconds @16:26:42.35 CS.seek 3ml @16:26:50.13 CSV.dial :waste @16:26:50.59 CS.seek 0ml @16:26:58.46 CSV.dial :flush @16:26:58.91 CS.seek 3ml @16:27:06.67 delay 5 seconds @16:27:11.68 CSV.dial :air @16:27:12.33 CS.seek 5ml @16:27:17.88 CSV.dial :air @16:27:17.97 CS.seek 0ml @16:27:30.48 delay 7 seconds @16:27:37.51 CS.seek 3ml @16:27:45.48 CSV.dial :waste @16:27:46.20 CS.seek 0ml @16:27:53.95 Garaging FlushPuck in Collection Clamp @16:27:54.02 Elbow.reconfigure :tight @16:27:54.07 Elbow.seek CC @16:27:54.18 Elbow.reconfigure :loose @16:27:54.29 Forearm.seek CC @16:27:55.26 Hand.close @16:27:55.95 CC.seek :open @16:28:00.44 Forearm.seek :retract @16:28:01.46 Elbow.reconfigure :tight @16:28:01.51 Elbow.seek :garage @16:28:04.39 Elbow.reconfigure :loose @16:28:04.50 Forearm.reconfigure :tight @16:28:04.55 Forearm.seek :garage @16:28:05.59 Hand.open @16:28:06.39 Forearm.reconfigure :loose @16:28:06.44 Forearm.seek :retract @16:28:07.43 da sampling at most 1000ml, wcr at most 1000ml @16:28:07.47 Loading sh1 puck from tube 3 into Collection Clamp @16:28:07.49 DAfans.email "Started da in tube 3 at 16:28:07UTC29-Oct-25\n{1 => 22,\n 2 => 14,\n 3 => 8,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@16:23:51, 41% flow, 19.1C, 23% humidity, 14.6psia, 13.68V, 0.309A avg, 217.582Ah, 4.23W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @16:28:07.78
SC.reconfigure :tight @16:28:07.83 SC.seek 3 @16:28:11.45 upsync @16:28:11.50
SC.configure :limp @16:28:11.74 Elbow.reconfigure :tight @16:28:11.79 Elbow.seek 3 @16:28:13.73 Forearm.reconfigure :tight @16:28:13.78 Forearm.seek 3 + 800 counts @16:28:14.85 SE.reconfigure :pick @16:28:14.92 SE.seek :beamBreak @16:28:17.21 SE.configure :default @16:28:17.38 Elbow.seek 3 @16:28:17.63 Forearm.seek 3 + 300 counts @16:28:17.91 Forearm.reconfigure :loose @16:28:17.96 Hand.close @16:28:18.71 SE.seek :down @16:28:19.28
Forearm.seek :retract @16:28:20.31 Elbow.seek CC @16:28:20.84 SE.stop @16:28:21.88
Elbow.reconfigure :loose @16:28:21.98 Forearm.reconfigure :tight @16:28:22.03 Forearm.seek CC @16:28:23.43 CC.seek :closed @16:28:27.45 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.09 @16:28:27.46 Hand.open @16:28:28.27 Forearm.reconfigure :loose @16:28:28.31 Forearm.seek :retract @16:28:29.26 collectSample 1000ml w/bubble point of 24psi @16:28:29.27 Priming 7ml @16:28:29.28 Intake.select :close @16:28:29.29 Exhaust.select :close @16:28:29.31 SS.hold @16:28:29.51 Calibrating pressure and temperature sensors @16:28:29.54 CTV.dial :air @16:28:29.56 SSV.dial :spit,avoiding: :exhaust @16:28:29.57 SS.reconfigure :fast @16:28:29.63 SS.seek 5ml @16:28:32.58 SSV.dial CBV @16:28:32.62
CBV.dial SSV @16:28:33.97 delay 3.8 seconds @16:28:37.90 Sensor limits: Intake max 97.1psia; Outlet max 110.2psia @16:28:37.91 SSV.dial :spit @16:28:39.26 SS.seek 0ml @16:28:41.95 SSV.dial CBV @16:28:43.30 CTV.dial :intake @16:28:44.06 SSV.dial CBV @16:28:44.08
CBV.dial SSV @16:28:44.22 Allowing 40s for 7.0ml of remaining 7.0ml @16:28:44.23 Intake.select :open @16:28:46.40 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:28:46.42 SS.reconfigure :inhaling 2.17ml/s @16:28:46.47 SS.seek 7ml @16:28:53.84 Stroke completed -- waiting for pressure to equalize ... @16:28:53.85 SS.reconfigure :exhale @16:28:53.90 SS.hold @16:28:54.24 Intake.select :close @16:28:54.40 SSV.dial :exhaust @16:28:55.16 Exhaust.select :open @16:28:55.27 SS.seek 0ml @16:28:59.01 Exhaust.select :close @16:28:59.12 Sampling 1000.0ml @16:28:59.13 Intake.select :close @16:28:59.14 Exhaust.select :close @16:28:59.15 SS.reconfigure :default @16:28:59.63 SS.hold @16:28:59.84 CTV.dial :intake @16:28:59.86 SSV.dial CBV @16:28:59.88
CBV.dial SSV @16:29:00.69 Allowing 130s for 25.0ml of remaining 1000.0ml @16:29:00.70 Intake.select :open @16:29:02.87 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:29:02.88 SS.reconfigure :inhaling 2.17ml/s @16:29:02.93 SS.seek 25ml @16:29:18.28 Stroke completed -- waiting for pressure to equalize ... @16:29:18.29 SS.reconfigure :exhale @16:29:18.34 SS.hold @16:29:23.96 Intake.select :close @16:29:24.11 SSV.dial :exhaust @16:29:24.87 Exhaust.select :open @16:29:24.98 SS.seek 0ml @16:29:33.86 Exhaust.select :close @16:29:33.97 SSV.dial CBV @16:29:34.00
CBV.dial SSV @16:29:34.78 Allowing 130s for 25.0ml of remaining 975.0ml @16:29:34.79 Intake.select :open @16:29:36.96 Intake @13.7psia, vacuum from 8.2 to 11.2psi @16:29:36.98 SS.reconfigure :inhaling 2.17ml/s @16:29:37.03 SS.seek 25ml @16:29:51.53 Stroke completed -- waiting for pressure to equalize ... @16:29:51.54 SS.reconfigure :exhale @16:29:51.59 SS.hold @16:29:58.15 Intake.select :close @16:29:58.31 SSV.dial :exhaust @16:29:59.06 Exhaust.select :open @16:29:59.18 SS.seek 0ml @16:30:08.08 Exhaust.select :close @16:30:08.19 SSV.dial CBV @16:30:08.21
CBV.dial SSV @16:30:09.01 Allowing 130s for 25.0ml of remaining 950.0ml @16:30:09.02 Intake.select :open @16:30:11.18 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:30:11.20 SS.reconfigure :inhaling 2.17ml/s @16:30:11.25 SS.seek 25ml @16:30:25.61 Stroke completed -- waiting for pressure to equalize ... @16:30:25.62 SS.reconfigure :exhale @16:30:25.66 SS.hold @16:30:33.12 Intake.select :close @16:30:33.28 SSV.dial :exhaust @16:30:34.06 Exhaust.select :open @16:30:34.17 SS.seek 0ml @16:30:43.36 Exhaust.select :close @16:30:43.47 SSV.dial CBV @16:30:43.49
CBV.dial SSV @16:30:44.28 Allowing 130s for 25.0ml of remaining 925.0ml @16:30:44.29 Intake.select :open @16:30:46.81 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:30:46.89 SS.reconfigure :inhaling 2.17ml/s @16:30:46.94 SS.seek 25ml @16:31:01.09 Stroke completed -- waiting for pressure to equalize ... @16:31:01.10 SS.reconfigure :exhale @16:31:01.15 SS.hold @16:31:08.64 Intake.select :close @16:31:08.80 SSV.dial :exhaust @16:31:09.58 Exhaust.select :open @16:31:09.69 SS.seek 0ml @16:31:18.53 Exhaust.select :close @16:31:18.64 SSV.dial CBV @16:31:18.66
CBV.dial SSV @16:31:19.47 Allowing 130s for 25.0ml of remaining 900.0ml @16:31:19.48 Intake.select :open @16:31:21.64 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:31:21.66 SS.reconfigure :inhaling 2.17ml/s @16:31:21.71 SS.seek 25ml @16:31:35.85 Stroke completed -- waiting for pressure to equalize ... @16:31:35.86 SS.reconfigure :exhale @16:31:35.91 SS.hold @16:31:44.82 Intake.select :close @16:31:44.98 SSV.dial :exhaust @16:31:45.76 Exhaust.select :open @16:31:45.87 SS.seek 0ml @16:31:55.14 Exhaust.select :close @16:31:55.25 SSV.dial CBV @16:31:55.27
CBV.dial SSV @16:31:56.07 Allowing 130s for 25.0ml of remaining 875.0ml @16:31:56.09 Intake.select :open @16:31:58.25 Intake @14.2psia, vacuum from 8.7 to 11.7psi @16:31:58.27 SS.reconfigure :inhaling 2.17ml/s @16:31:58.32 SS.seek 25ml @16:32:12.23 Stroke completed -- waiting for pressure to equalize ... @16:32:12.24 SS.reconfigure :exhale @16:32:12.29 SS.hold @16:32:21.34 Intake.select :close @16:32:21.49 SSV.dial :exhaust @16:32:22.26 Exhaust.select :open @16:32:22.38 SS.seek 0ml @16:32:31.64 Exhaust.select :close @16:32:31.75 SSV.dial CBV @16:32:31.77
CBV.dial SSV @16:32:32.56 Allowing 130s for 25.0ml of remaining 850.0ml @16:32:32.57 Intake.select :open @16:32:35.11 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:32:35.12 SS.reconfigure :inhaling 2.17ml/s @16:32:35.17 SS.seek 25ml @16:32:49.07 Stroke completed -- waiting for pressure to equalize ... @16:32:49.08 SS.reconfigure :exhale @16:32:49.13 SS.hold @16:32:58.86 Intake.select :close @16:32:59.01 SSV.dial :exhaust @16:32:59.79 Exhaust.select :open @16:32:59.90 SS.seek 0ml @16:33:09.15 Exhaust.select :close @16:33:09.26 SSV.dial CBV @16:33:09.30
CBV.dial SSV @16:33:10.06 Allowing 130s for 25.0ml of remaining 825.0ml @16:33:10.07 Intake.select :open @16:33:12.24 Intake @14.2psia, vacuum from 8.7 to 11.7psi @16:33:12.26 SS.reconfigure :inhaling 2.17ml/s @16:33:12.31 SS.seek 25ml @16:33:26.23 Stroke completed -- waiting for pressure to equalize ... @16:33:26.24 SS.reconfigure :exhale @16:33:26.28 SS.hold @16:33:36.10 Intake.select :close @16:33:36.26 SSV.dial :exhaust @16:33:37.05 Exhaust.select :open @16:33:37.16 SS.seek 0ml @16:33:46.38 Exhaust.select :close @16:33:46.49 SSV.dial CBV @16:33:46.51
CBV.dial SSV @16:33:47.32 Allowing 130s for 25.0ml of remaining 800.0ml @16:33:47.33 Intake.select :open @16:33:49.49 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:33:49.51 SS.reconfigure :inhaling 2.17ml/s @16:33:49.56 SS.seek 25ml @16:34:03.79 Stroke completed -- waiting for pressure to equalize ... @16:34:03.80 SS.reconfigure :exhale @16:34:03.85 SS.hold @16:34:15.39 Intake.select :close @16:34:15.55 SSV.dial :exhaust @16:34:16.31 Exhaust.select :open @16:34:16.42 SS.seek 0ml @16:34:25.68 Exhaust.select :close @16:34:25.79 SSV.dial CBV @16:34:25.83
CBV.dial SSV @16:34:26.59 Allowing 130s for 25.0ml of remaining 775.0ml @16:34:26.60 Intake.select :open @16:34:28.78 Intake @14.2psia, vacuum from 8.7 to 11.7psi @16:34:28.79 SS.reconfigure :inhaling 2.17ml/s @16:34:28.84 SS.seek 25ml @16:34:42.79 Stroke completed -- waiting for pressure to equalize ... @16:34:42.80 SS.reconfigure :exhale @16:34:42.85 SS.hold @16:34:54.01 Intake.select :close @16:34:54.17 SSV.dial :exhaust @16:34:54.93 Exhaust.select :open @16:34:55.04 SS.seek 0ml @16:35:03.91 Exhaust.select :close @16:35:04.02 SSV.dial CBV @16:35:04.04
CBV.dial SSV @16:35:04.84 Allowing 130s for 25.0ml of remaining 750.0ml @16:35:04.85 Intake.select :open @16:35:07.02 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:35:07.03 SS.reconfigure :inhaling 2.17ml/s @16:35:07.08 SS.seek 25ml @16:35:20.97 Stroke completed -- waiting for pressure to equalize ... @16:35:20.98 SS.reconfigure :exhale @16:35:21.03 SS.hold @16:35:33.96 Intake.select :close @16:35:34.12 SSV.dial :exhaust @16:35:34.90 Exhaust.select :open @16:35:35.01 SS.seek 0ml @16:35:43.90 Exhaust.select :close @16:35:44.01 SSV.dial CBV @16:35:44.03
CBV.dial SSV @16:35:44.84 Allowing 130s for 25.0ml of remaining 725.0ml @16:35:44.85 Intake.select :open @16:35:47.01 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:35:47.03 SS.reconfigure :inhaling 2.17ml/s @16:35:47.08 SS.seek 25ml @16:36:00.44 Vacuum too high -- waiting up to 40 seconds @16:36:00.45 SS.reconfigure :exhale @16:36:00.50 SS.hold @16:36:01.19 Intake @11.3psia, vacuum from 5.8 to 8.8psi @16:36:01.21 SS.reconfigure :inhaling 1.95ml/s @16:36:01.26 SS.seek 25ml @16:36:02.08 Stroke completed -- waiting for pressure to equalize ... @16:36:02.09 SS.reconfigure :exhale @16:36:02.14 SS.hold @16:36:16.63 Intake.select :close @16:36:16.78 SSV.dial :exhaust @16:36:17.57 Exhaust.select :open @16:36:17.68 SS.seek 0ml @16:36:26.99 Exhaust.select :close @16:36:27.10 SSV.dial CBV @16:36:27.13
CBV.dial SSV @16:36:27.91 Allowing 130s for 25.0ml of remaining 700.0ml @16:36:27.92 Intake.select :open @16:36:30.09 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:36:30.11 SS.reconfigure :inhaling 1.95ml/s @16:36:30.16 SS.seek 25ml @16:36:45.13 Stroke completed -- waiting for pressure to equalize ... @16:36:45.14 SS.reconfigure :exhale @16:36:45.19 SS.hold @16:37:01.48 Intake.select :close @16:37:01.64 SSV.dial :exhaust @16:37:02.41 Exhaust.select :open @16:37:02.53 SS.seek 0ml @16:37:11.80 Exhaust.select :close @16:37:11.91 SSV.dial CBV @16:37:11.94
CBV.dial SSV @16:37:12.72 Allowing 130s for 25.0ml of remaining 675.0ml @16:37:12.74 Intake.select :open @16:37:14.90 Intake @14.3psia, vacuum from 8.8 to 11.8psi @16:37:14.92 SS.reconfigure :inhaling 1.95ml/s @16:37:14.97 SS.seek 25ml @16:37:27.21 Vacuum too high -- waiting up to 40 seconds @16:37:27.23 SS.reconfigure :exhale @16:37:27.28 SS.hold @16:37:28.84 Intake @11.8psia, vacuum from 6.3 to 9.3psi @16:37:28.85 SS.reconfigure :inhaling 1.77ml/s @16:37:28.90 SS.seek 25ml @16:37:31.25 Vacuum too high -- waiting up to 40 seconds @16:37:31.26 SS.reconfigure :exhale @16:37:31.31 SS.hold @16:37:43.17 Intake @12.8psia, vacuum from 7.3 to 10.3psi @16:37:43.19 SS.reconfigure :inhaling 1.59ml/s @16:37:43.24 SS.seek 25ml @16:37:45.13 Stroke completed -- waiting for pressure to equalize ... @16:37:45.14 SS.reconfigure :exhale @16:37:45.19 SS.hold @16:37:48.16 Intake.select :close @16:37:48.32 SSV.dial :exhaust @16:37:49.10 Exhaust.select :open @16:37:49.21 SS.seek 0ml @16:37:58.41 Exhaust.select :close @16:37:58.52 SSV.dial CBV @16:37:58.55
CBV.dial SSV @16:37:59.33 Allowing 130s for 25.0ml of remaining 650.0ml @16:37:59.34 Intake.select :open @16:38:01.50 Intake @14.1psia, vacuum from 8.6 to 11.6psi @16:38:01.52 SS.reconfigure :inhaling 1.59ml/s @16:38:01.57 SS.seek 25ml @16:38:13.21 Vacuum too high -- waiting up to 40 seconds @16:38:13.23 SS.reconfigure :exhale @16:38:13.28 SS.hold @16:38:20.08 Intake @12.5psia, vacuum from 7.0 to 10.0psi @16:38:20.10 SS.reconfigure :inhaling 1.43ml/s @16:38:20.15 SS.seek 25ml @16:38:26.90 Vacuum too high -- waiting up to 40 seconds @16:38:26.92 SS.reconfigure :exhale @16:38:26.97 SS.hold @16:38:33.58 Intake @12.7psia, vacuum from 7.2 to 10.2psi @16:38:33.60 SS.reconfigure :inhaling 1.29ml/s @16:38:33.65 SS.seek 25ml @16:38:34.28 Stroke completed -- waiting for pressure to equalize ... @16:38:34.29 SS.reconfigure :exhale @16:38:34.34 SS.hold @16:38:36.72 Intake.select :close @16:38:36.88 SSV.dial :exhaust @16:38:37.65 Exhaust.select :open @16:38:38.15 SS.seek 0ml @16:38:47.27 Exhaust.select :close @16:38:47.38 SSV.dial CBV @16:38:47.41
CBV.dial SSV @16:38:48.21 Allowing 130s for 25.0ml of remaining 625.0ml @16:38:48.22 Intake.select :open @16:38:50.39 Intake @14.5psia, vacuum from 9.0 to 12.0psi @16:38:50.40 SS.reconfigure :inhaling 1.29ml/s @16:38:50.45 SS.seek 25ml @16:39:05.37 Vacuum too high -- waiting up to 40 seconds @16:39:05.38 SS.reconfigure :exhale @16:39:05.44 SS.hold @16:39:06.17 Intake @11.5psia, vacuum from 6.0 to 9.0psi @16:39:06.19 SS.reconfigure :inhaling 1.16ml/s @16:39:06.24 SS.seek 25ml @16:39:08.37 Vacuum too high -- waiting up to 40 seconds @16:39:08.38 SS.reconfigure :exhale @16:39:08.44 SS.hold @16:39:18.35 Intake @12.8psia, vacuum from 7.3 to 10.3psi @16:39:18.36 SS.reconfigure :inhaling 1.04ml/s @16:39:18.41 SS.seek 25ml @16:39:25.34 Stroke completed -- waiting for pressure to equalize ... @16:39:25.35 SS.reconfigure :exhale @16:39:25.40 SS.hold @16:39:31.99 Intake.select :close @16:39:32.15 SSV.dial :exhaust @16:39:32.91 Exhaust.select :open @16:39:33.02 SS.seek 0ml @16:39:42.28 Exhaust.select :close @16:39:42.39 SSV.dial CBV @16:39:42.41
CBV.dial SSV @16:39:43.21 Allowing 130s for 25.0ml of remaining 600.0ml @16:39:43.22 Intake.select :open @16:39:45.39 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:39:45.40 SS.reconfigure :inhaling 1.04ml/s @16:39:45.45 SS.seek 25ml @16:40:03.93 Vacuum too high -- waiting up to 40 seconds @16:40:03.94 SS.reconfigure :exhale @16:40:04.00 SS.hold @16:40:09.74 Intake @13.0psia, vacuum from 7.5 to 10.5psi @16:40:09.76 SS.reconfigure :inhaling 0.94ml/s @16:40:09.81 SS.seek 25ml @16:40:17.92 Stroke completed -- waiting for pressure to equalize ... @16:40:17.93 SS.reconfigure :exhale @16:40:17.98 SS.hold @16:40:29.40 Intake.select :close @16:40:29.55 SSV.dial :exhaust @16:40:30.33 Exhaust.select :open @16:40:30.45 SS.seek 0ml @16:40:39.61 Exhaust.select :close @16:40:39.72 SSV.dial CBV @16:40:39.74
CBV.dial SSV @16:40:40.53 Allowing 130s for 25.0ml of remaining 575.0ml @16:40:40.54 Intake.select :open @16:40:42.70 Intake @14.6psia, vacuum from 9.1 to 12.1psi @16:40:42.72 SS.reconfigure :inhaling 0.94ml/s @16:40:42.77 SS.seek 25ml @16:40:58.12 Vacuum too high -- waiting up to 40 seconds @16:40:58.13 SS.reconfigure :exhale @16:40:58.18 SS.hold @16:41:03.93 Intake @13.6psia, vacuum from 8.1 to 11.1psi @16:41:03.95 SS.reconfigure :inhaling 0.85ml/s @16:41:04.00 SS.seek 25ml @16:41:14.18 Vacuum too high -- waiting up to 40 seconds @16:41:14.19 SS.reconfigure :exhale @16:41:14.25 SS.hold @16:41:19.99 Intake @13.5psia, vacuum from 8.0 to 11.0psi @16:41:20.01 SS.reconfigure :inhaling 0.77ml/s @16:41:20.06 SS.seek 25ml @16:41:24.88 Stroke completed -- waiting for pressure to equalize ... @16:41:24.89 SS.reconfigure :exhale @16:41:24.94 SS.hold @16:41:31.43 Intake.select :close @16:41:31.59 SSV.dial :exhaust @16:41:32.34 Exhaust.select :open @16:41:32.45 SS.seek 0ml @16:41:41.34 Exhaust.select :close @16:41:41.45 SSV.dial CBV @16:41:41.47
CBV.dial SSV @16:41:42.27 Allowing 130s for 25.0ml of remaining 550.0ml @16:41:42.28 Intake.select :open @16:41:44.45 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:41:44.46 SS.reconfigure :inhaling 0.77ml/s @16:41:44.51 SS.seek 25ml @16:42:07.18 Vacuum too high -- waiting up to 40 seconds @16:42:07.19 SS.reconfigure :exhale @16:42:07.25 SS.hold @16:42:12.99 Intake @13.3psia, vacuum from 7.8 to 10.8psi @16:42:13.00 SS.reconfigure :inhaling 0.70ml/s @16:42:13.05 SS.seek 25ml @16:42:26.14 Stroke completed -- waiting for pressure to equalize ... @16:42:26.15 SS.reconfigure :exhale @16:42:26.20 SS.hold @16:42:37.38 Intake.select :close @16:42:37.54 SSV.dial :exhaust @16:42:38.31 Exhaust.select :open @16:42:38.42 SS.seek 0ml @16:42:47.83 Exhaust.select :close @16:42:47.94 SSV.dial CBV @16:42:47.96
CBV.dial SSV @16:42:48.78 Allowing 130s for 25.0ml of remaining 525.0ml @16:42:48.79 Intake.select :open @16:42:50.96 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:42:50.98 SS.reconfigure :inhaling 0.70ml/s @16:42:51.03 SS.seek 25ml @16:43:11.33 Vacuum too high -- waiting up to 40 seconds @16:43:11.35 SS.reconfigure :exhale @16:43:11.40 SS.hold @16:43:17.13 Intake @13.8psia, vacuum from 8.3 to 11.3psi @16:43:17.15 SS.reconfigure :inhaling 0.62ml/s @16:43:17.20 SS.seek 25ml @16:43:35.48 Vacuum too high -- waiting up to 40 seconds @16:43:35.49 SS.reconfigure :exhale @16:43:35.55 SS.hold @16:43:42.16 Intake @13.9psia, vacuum from 8.4 to 11.4psi @16:43:42.18 SS.reconfigure :inhaling 0.56ml/s @16:43:42.23 SS.seek 25ml @16:43:43.89 Stroke completed -- waiting for pressure to equalize ... @16:43:43.90 SS.reconfigure :exhale @16:43:43.94 SS.hold @16:43:48.80 Intake.select :close @16:43:48.96 SSV.dial :exhaust @16:43:49.73 Exhaust.select :open @16:43:49.84 SS.seek 0ml @16:43:59.15 Exhaust.select :close @16:43:59.26 SSV.dial CBV @16:43:59.30
CBV.dial SSV @16:44:00.06 Allowing 130s for 25.0ml of remaining 500.0ml @16:44:00.07 Intake.select :open @16:44:02.24 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:44:02.26 SS.reconfigure :inhaling 0.56ml/s @16:44:02.31 SS.seek 25ml @16:44:32.43 Vacuum too high -- waiting up to 40 seconds @16:44:32.44 SS.reconfigure :exhale @16:44:32.49 SS.hold @16:44:38.13 Intake @13.6psia, vacuum from 8.1 to 11.1psi @16:44:38.15 SS.reconfigure :inhaling 0.51ml/s @16:44:38.20 SS.seek 25ml @16:44:55.96 Stroke completed -- waiting for pressure to equalize ... @16:44:55.97 SS.reconfigure :exhale @16:44:56.02 SS.hold @16:45:08.60 Intake.select :close @16:45:08.76 SSV.dial :exhaust @16:45:09.54 Exhaust.select :open @16:45:09.65 SS.seek 0ml @16:45:18.90 Exhaust.select :close @16:45:19.01 SSV.dial CBV @16:45:19.04
CBV.dial SSV @16:45:19.82 Allowing 130s for 25.0ml of remaining 475.0ml @16:45:19.83 Intake.select :open @16:45:22.00 Intake @14.9psia, vacuum from 9.4 to 12.4psi @16:45:22.01 SS.reconfigure :inhaling 0.51ml/s @16:45:22.06 SS.seek 25ml @16:45:59.08 Vacuum too high -- waiting up to 40 seconds @16:45:59.09 SS.reconfigure :exhale @16:45:59.15 SS.hold @16:46:05.76 Intake @13.6psia, vacuum from 8.1 to 11.1psi @16:46:05.78 SS.reconfigure :inhaling 0.46ml/s @16:46:05.82 SS.seek 25ml @16:46:21.46 Stroke completed -- waiting for pressure to equalize ... @16:46:21.47 SS.reconfigure :exhale @16:46:21.52 SS.hold @16:46:36.09 Intake.select :close @16:46:36.24 SSV.dial :exhaust @16:46:37.01 Exhaust.select :open @16:46:37.13 SS.seek 0ml @16:46:46.48 Exhaust.select :close @16:46:46.59 SSV.dial CBV @16:46:46.61
CBV.dial SSV @16:46:47.43 Allowing 130s for 25.0ml of remaining 450.0ml @16:46:47.44 Intake.select :open @16:46:49.60 Intake @15.0psia, vacuum from 9.5 to 12.5psi @16:46:49.62 SS.reconfigure :inhaling 0.46ml/s @16:46:49.67 SS.seek 25ml @16:47:32.30 Vacuum too high -- waiting up to 40 seconds @16:47:32.31 SS.reconfigure :exhale @16:47:32.36 SS.hold @16:47:38.91 Intake @13.8psia, vacuum from 8.3 to 11.3psi @16:47:38.92 SS.reconfigure :inhaling 0.42ml/s @16:47:38.97 SS.seek 25ml @16:47:53.32 Stroke completed -- waiting for pressure to equalize ... @16:47:53.33 SS.reconfigure :exhale @16:47:53.38 SS.hold @16:48:09.73 Intake.select :close @16:48:09.88 SSV.dial :exhaust @16:48:10.65 Exhaust.select :open @16:48:10.76 SS.seek 0ml @16:48:20.27 Exhaust.select :close @16:48:20.38 SSV.dial CBV @16:48:20.41
CBV.dial SSV @16:48:21.19 Allowing 130s for 25.0ml of remaining 425.0ml @16:48:21.21 Intake.select :open @16:48:23.79 Intake @15.1psia, vacuum from 9.6 to 12.6psi @16:48:23.82 SS.reconfigure :inhaling 0.42ml/s @16:48:23.87 SS.seek 25ml @16:49:02.76 Vacuum too high -- waiting up to 40 seconds @16:49:02.78 SS.reconfigure :exhale @16:49:02.83 SS.hold @16:49:10.41 Intake @14.4psia, vacuum from 8.9 to 11.9psi @16:49:10.43 SS.reconfigure :inhaling 0.38ml/s @16:49:10.48 SS.seek 25ml @16:49:35.68 Stroke completed -- waiting for pressure to equalize ... @16:49:35.69 SS.reconfigure :exhale @16:49:35.73 SS.hold @16:49:52.56 Intake.select :close @16:49:52.72 SSV.dial :exhaust @16:49:53.47 Exhaust.select :open @16:49:53.59 SS.seek 0ml @16:50:03.05 Exhaust.select :close @16:50:03.16 SSV.dial CBV @16:50:03.20
CBV.dial SSV @16:50:03.96 Allowing 130s for 25.0ml of remaining 400.0ml @16:50:03.97 Intake.select :open @16:50:06.14 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:50:06.15 SS.reconfigure :inhaling 0.38ml/s @16:50:06.20 SS.seek 25ml @16:50:47.10 Vacuum too high -- waiting up to 40 seconds @16:50:47.12 SS.reconfigure :exhale @16:50:47.17 SS.hold @16:51:01.55 Intake @14.8psia, vacuum from 9.3 to 12.3psi @16:51:01.57 SS.reconfigure :inhaling 0.33ml/s @16:51:01.62 SS.seek 25ml @16:51:26.67 Vacuum too high -- waiting up to 40 seconds @16:51:26.68 SS.reconfigure :exhale @16:51:26.74 SS.hold @16:51:45.16 Intake @14.7psia, vacuum from 9.2 to 12.2psi @16:51:45.18 SS.reconfigure :inhaling 0.30ml/s @16:51:45.22 SS.seek 25ml @16:51:52.58 Stroke completed -- waiting for pressure to equalize ... @16:51:52.59 SS.reconfigure :exhale @16:51:52.64 SS.hold @16:52:13.98 SS.reconfigure :default @16:52:14.03 SS.hold @16:52:14.10 Intake.select :close @16:52:14.26 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 625.0ml @16:52:14.27 SSV.dial :exhaust @16:52:15.05 Exhaust.select :open @16:52:15.16 SS.reconfigure :exhale @16:52:15.21 SS.seek 0ml @16:52:24.21 Exhaust.select :close @16:52:24.31 Sampled 625.0ml @16:52:24.33 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @16:52:24.34 Intake.select :close @16:52:24.41 CSV.dial :waste @16:52:24.56 SSV.dial CBV,avoiding: :exhaust @16:52:24.59 CS.seek 0ml @16:52:24.61
CBV.dial SSV @16:52:24.76 CSV.dial :air @16:52:25.51 CS.seek 10ml @16:52:26.57
CTV.dial :air @16:52:27.35 delay 15 seconds @16:52:42.37 SSV.dial :spit,avoiding: :exhaust @16:52:43.71 CTV.dial CSV,avoiding: :intake @16:52:43.75
SS.seek 0ml @16:52:43.88 SSV.dial CBV,avoiding: :exhaust @16:52:49.77 SSV.dial between CBV and plug @16:52:50.68 CBV.dial between SSV and waste2 @16:52:51.07 CTV.dial CSV,avoiding: :air @16:52:51.10
CSV.dial CTV @16:52:51.59 CS.reconfigure :pressurize @16:52:51.64 CS.seek 0ml @16:53:15.03 BubblePoint: Intake @24.2psia, Outlet @0.1psia @16:53:15.04 CBV.dial SSV @16:53:15.38 SS.reconfigure :default @16:53:15.44 SS.seek 10ml @16:53:21.02 delay 15 seconds @16:53:36.15 Evacuated: Intake @23.9psia, Outlet @23.2psia @16:53:36.16 SSV.dial :spit,avoiding: :exhaust @16:53:37.23 SS.reconfigure :exhale @16:53:37.27 SS.seek 0ml @16:53:42.39 SS.reconfigure :fast @16:53:42.49 SS.seek 5ml @16:53:45.44 SSV.dial CBV,avoiding: :exhaust @16:53:45.47
CBV.dial SSV @16:53:46.85 CTV.dial :air,avoiding: :intake @16:53:48.24 CSV.dial CTV @16:53:48.28
SS.seek 10ml @16:53:49.40 CSV.dial :air @16:53:49.87 CS.reconfigure :fast @16:53:49.92 CS.seek 0ml @16:53:50.92
SSV.dial :spit,avoiding: :exhaust @16:53:52.28 SS.reconfigure :exhale @16:53:52.33 SS.seek 0ml @16:53:53.23 CS.stop @16:53:57.21
SS.reconfigure :fast @16:53:57.26 SS.seek 5ml @16:54:00.18 SSV.dial CBV,avoiding: :exhaust @16:54:00.20
CBV.dial SSV @16:54:02.59 SSV.dial :spit,avoiding: :exhaust @16:54:03.93 SS.reconfigure :default @16:54:03.98 SS.seek 0ml @16:54:06.98 SS.stop @16:54:07.03 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @16:54:07.04 Intake.select :close @16:54:07.11 CSV.dial :waste @16:54:07.24 SSV.dial CBV,avoiding: :exhaust @16:54:07.27
CBV.dial SSV @16:54:07.87 CS.seek 0ml @16:54:07.95 CSV.dial :air @16:54:08.63
CTV.dial :air @16:54:08.71 CS.seek 10ml @16:54:09.20
delay 15 seconds @16:54:24.22 SSV.dial :spit,avoiding: :exhaust @16:54:25.57 CTV.dial CSV,avoiding: :intake @16:54:25.61
SS.reconfigure :exhale @16:54:25.71 SS.seek 0ml @16:54:26.22 SSV.dial CBV,avoiding: :exhaust @16:54:32.80 SSV.dial between CBV and plug @16:54:33.30 CBV.dial between SSV and waste2 @16:54:33.68 CSV.dial between air and CTV @16:54:33.99 CS.seek 7.949ml @16:54:39.55 CTV.dial CSV,avoiding: :air @16:54:39.58
CSV.dial CTV @16:54:39.93 CS.reconfigure :pressurize @16:54:39.98 CS.seek 0ml @16:54:58.46 BubblePoint: Intake @24.1psia, Outlet @-0.2psia @16:54:58.47 CBV.dial SSV @16:54:58.82 SS.reconfigure :default @16:54:58.87 SS.seek 10ml @16:55:04.42 delay 15 seconds @16:55:19.56 Evacuated: Intake @22.9psia, Outlet @16.8psia @16:55:19.57 SSV.dial :spit,avoiding: :exhaust @16:55:20.61 SS.reconfigure :exhale @16:55:20.66 SS.seek 0ml @16:55:25.74 SS.reconfigure :fast @16:55:25.79 SS.seek 5ml @16:55:28.72 SSV.dial CBV,avoiding: :exhaust @16:55:28.76
CBV.dial SSV @16:55:30.10 CTV.dial :air,avoiding: :intake @16:55:31.47 CSV.dial CTV @16:55:31.51
SS.seek 10ml @16:55:32.64 CSV.dial :air @16:55:33.10 CS.reconfigure :fast @16:55:33.15 CS.seek 0ml @16:55:34.20
SSV.dial :spit,avoiding: :exhaust @16:55:35.55 SS.reconfigure :exhale @16:55:35.60 SS.seek 0ml @16:55:36.14 CS.stop @16:55:40.59
SS.reconfigure :fast @16:55:40.64 SS.seek 5ml @16:55:43.44 SSV.dial CBV,avoiding: :exhaust @16:55:43.47
CBV.dial SSV @16:55:45.85 SSV.dial :spit,avoiding: :exhaust @16:55:47.19 SS.reconfigure :default @16:55:47.24 SS.seek 0ml @16:55:50.64 SS.stop @16:55:50.68 Lysing: Adding 2.0ml AqMeOH @16:55:50.69 CBV.dial between CSV and waste1,avoiding: :DBV @16:55:52.20 CTV.dial CSV @16:55:53.55 CSV.dial CSR @16:55:54.34 Pulling 1ml of CSR.AqMeOH @16:55:54.35 CSR.select :AqMeOH @16:55:54.46 CS.reconfigure :default @16:55:54.51 CS.seek 1ml @16:55:57.87 delay 5 seconds @16:56:02.89 CSR.select :air @16:56:03.06 CS.seek 6ml @16:56:16.70 delay 2 seconds @16:56:18.72 CSV.dial :waste2 @16:56:19.16 CS.reconfigure :fast @16:56:19.21 CS.seek 0ml @16:56:33.67 CSV.dial CSR @16:56:34.25 Pulling 2ml of CSR.AqMeOH @16:56:34.26 CSR.select :AqMeOH @16:56:34.38 CS.reconfigure :default @16:56:34.43 CS.seek 2ml @16:56:40.36 delay 5 seconds @16:56:45.38 CSR.select :air @16:56:45.55 CS.seek 5ml @16:56:54.33 delay 2 seconds @16:56:56.34 CSV.dial CTV @16:56:56.82 CS.seek 1ml @16:57:08.16 Before lysing Collection Puck Heater at 15.7C/15.8psia @16:57:08.18 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @16:57:08.19 CH.seek 70C,8:00 @16:59:31.51 CH.hold 10:00,70C @16:59:31.72 Begun lysing Collection Puck Heater at 68C/22.4psia @17:09:31.73 Ended lysing Collection Puck Heater at 70C/19.4psia @17:09:31.74 CS.seek 0ml @17:09:35.27 CTV.dial between spare and air,avoiding: :intake @17:09:36.35 CSV.dial :air @17:09:36.80 CS.seek 5ml @17:09:50.38 CSV.dial CTV @17:09:50.83 delay 2 seconds @17:09:52.85 CSV.dial :air @17:09:53.30 delay 2 seconds @17:09:55.32 CSV.dial CTV @17:09:55.78 delay 2 seconds @17:09:57.80 CSV.dial :air @17:09:58.26 delay 2 seconds @17:10:00.28 CS.seek 0ml @17:10:13.99 Puck @27.8psia after final lysis pressurization @17:10:14.01 Recovering Lysate into Collection syringe @17:10:14.02 CSV.dial CBV @17:10:15.02 CBV.dial CSV @17:10:15.41 CS.seek 5ml @17:10:29.19 delay 5 seconds @17:10:34.21 CTV.dial :air @17:10:34.75 CS.seek 7ml @17:10:40.75 delay 5 seconds @17:10:45.77 CSV.dial :air2 @17:10:46.23 CS.seek 10ml @17:10:54.72 CSV.dial between air and CTV,avoiding: CBV @17:10:55.87 Lysing Complete @17:10:55.94 Storing sh1 puck in Collection Clamp to tube 2 @17:10:56.11 SC.reconfigure :tight @17:10:56.16 SC.seek 2 @17:10:59.69 SC.configure :limp @17:10:59.92 Elbow.reconfigure :tight @17:10:59.97 Elbow.seek 2 @17:11:01.67 Forearm.reconfigure :tight @17:11:01.72 Forearm.seek 2 + 800 counts @17:11:02.85 SE.reconfigure :pick @17:11:02.91 SE.seek :beamBreak @17:11:04.64 SE.configure :default @17:11:04.81 Elbow.reconfigure :limp @17:11:04.86 Forearm.reconfigure :loose @17:11:04.90 Forearm.seek 2 @17:11:05.26 Elbow.hold @17:11:05.77 Elbow.reconfigure :tight @17:11:05.87 SE.seek 13.09 Pucks below Top Plate @17:11:06.30 Forearm.force -20 @17:11:06.34 Forearm.seek 2 + 313 counts @17:11:06.70 Forearm.reconfigure :tight @17:11:06.75 Forearm.seek 2 + 800 counts @17:11:07.06 SE.seek 14.09 Pucks below Top Plate @17:11:07.77 Forearm.reconfigure :loose @17:11:07.82 Forearm.seek :retract @17:11:08.80 Elbow.seek CC @17:11:10.46 Elbow.reconfigure :loose @17:11:10.56 Forearm.seek CC @17:11:11.54 Hand.close @17:11:12.28 CC.seek :open @17:11:16.52 Forearm.seek :retract @17:11:17.58 Elbow.reconfigure :tight @17:11:17.62 Elbow.seek 2 @17:11:19.34 Elbow.reconfigure :limp @17:11:19.40 Forearm.seek 2 @17:11:20.56 Elbow.hold @17:11:20.62 Elbow.reconfigure :tight @17:11:20.67 Hand.open @17:11:21.53 Forearm.force -20 @17:11:21.57 Forearm.seek 2 + 313 counts @17:11:21.93 Forearm.reconfigure :tight @17:11:21.98 Forearm.seek 2 + 800 counts @17:11:22.37 SE.seek :down @17:11:23.96 SE.stop @17:11:23.99
Forearm.reconfigure :loose @17:11:24.08 Forearm.seek :retract @17:11:25.03 Tranferring DA @17:11:25.05 Beginning samplePassC @17:11:25.06 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:11:25.25 Elbow.seek FlushPuck::Garage @17:11:27.01 Elbow.reconfigure :loose @17:11:27.12 Forearm.seek :garage @17:11:28.17 Hand.close @17:11:29.02 Forearm.seek :retract @17:11:30.01 Elbow.reconfigure :tight @17:11:30.07 Elbow.seek CC @17:11:32.92 Elbow.reconfigure :loose @17:11:33.02 Forearm.reconfigure :tight @17:11:33.07 Forearm.seek CC @17:11:34.41 CC.seek :closed @17:11:38.38 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.96 @17:11:38.45 Hand.open @17:11:39.22 Forearm.reconfigure :loose @17:11:39.27 Forearm.seek :retract @17:11:40.35 PSV.dial PSR @17:11:41.74 Pulling 1.5ml of PSR.flush @17:11:41.75 PSR.select :flush @17:11:41.87 PS.seek 1.5ml @17:11:58.05 delay 5 seconds @17:12:03.07 PSV.dial :waste1 @17:12:03.56 PS.seek 0ml @17:12:20.15 PSV.dial PSR @17:12:20.62 PSR.select :air @17:12:20.73 PS.seek 2.5ml @17:12:47.03 PRV.dial CBV,avoiding: :ab2 @17:12:47.05
CBV.dial PRV @17:12:48.84 PSV.dial :mixloop @17:12:49.87 CTV.dial :air,avoiding: :intake @17:12:49.94 PTV.dial :PRVmixing @17:12:51.26 PS.seek 0ml @17:13:17.61 PSV.dial :air @17:13:18.09 PS.seek 2.5ml @17:13:44.39 PSV.dial :mixloop @17:13:44.87 PS.seek 0ml @17:14:11.39 PSV.dial :air @17:14:11.87 PS.seek 2.5ml @17:14:38.24 PSV.dial :mixloop @17:14:38.71 PS.seek 0ml @17:15:05.05 Garaging FlushPuck in Collection Clamp @17:15:05.13 Elbow.reconfigure :tight @17:15:05.18 Elbow.seek CC @17:15:05.30 Elbow.reconfigure :loose @17:15:05.41 Forearm.seek CC @17:15:06.34 Hand.close @17:15:07.03 CC.seek :open @17:15:11.12 Forearm.seek :retract @17:15:12.10 Elbow.reconfigure :tight @17:15:12.15 Elbow.seek :garage @17:15:15.03 Elbow.reconfigure :loose @17:15:15.14 Forearm.reconfigure :tight @17:15:15.19 Forearm.seek :garage @17:15:16.32 Hand.open @17:15:17.08 Forearm.reconfigure :loose @17:15:17.13 Forearm.seek :retract @17:15:18.13 PSV.dial :mixloop @17:15:18.59 PTV.dial :PRVmixing @17:15:18.73 PRV.dial CBV @17:15:18.75
CBV.dial PRV @17:15:18.98 CTV.dial :air,avoiding: :intake @17:15:19.00 Loading lyfil puck from tube 3 into Collection Clamp @17:15:19.26 SC.reconfigure :tight @17:15:19.31 SC.seek 3 @17:15:22.95 SC.configure :limp @17:15:23.18 Elbow.reconfigure :tight @17:15:23.23 Elbow.seek 3 @17:15:25.11 Forearm.reconfigure :tight @17:15:25.16 Forearm.seek 3 + 800 counts @17:15:26.30 SE.reconfigure :pick @17:15:26.36 SE.seek :beamBreak @17:15:28.73 SE.configure :default @17:15:28.90 Elbow.seek 3 @17:15:29.08 Forearm.seek 3 + 300 counts @17:15:29.37 Forearm.reconfigure :loose @17:15:29.41 Hand.close @17:15:30.16 SE.seek :down @17:15:30.38
Forearm.seek :retract @17:15:31.42 Elbow.seek CC @17:15:32.39 SE.stop @17:15:33.00
Elbow.reconfigure :loose @17:15:33.11 Forearm.reconfigure :tight @17:15:33.16 Forearm.seek CC @17:15:34.89 CC.seek :closed @17:15:38.91 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.95 @17:15:38.92 Hand.open @17:15:39.71 Forearm.reconfigure :loose @17:15:39.76 Forearm.seek :retract @17:15:40.74 CTV.dial CSV,avoiding: :intake @17:15:42.11 CSV.dial CTV @17:15:42.43 PS.seek 2.5ml @17:16:08.89 CS.seek 4.999ml @17:16:22.47 delay 8 seconds @17:16:30.49 PSV.dial :air @17:16:30.96 delay 2 seconds @17:16:32.97 PSV.dial :waste2 @17:16:33.84 PS.seek 0ml @17:17:00.24 CBV.dial between PRV and waste1 @17:17:00.56 CSV.dial :air @17:17:01.02 CS.seek 10ml @17:17:14.65 CSV.dial CTV @17:17:15.11 CS.seek 0ml @17:17:41.56 CBV.dial :waste1 @17:17:41.87 delay 5 seconds @17:17:46.90 CTV.dial :air,avoiding: :intake @17:17:48.31 Storing lyfil puck in Collection Clamp to tube 2 @17:17:48.48 SC.reconfigure :tight @17:17:48.53 SC.seek 2 @17:17:52.19 SC.configure :limp @17:17:52.41 Elbow.reconfigure :tight @17:17:52.46 Elbow.seek 2 @17:17:54.33 Forearm.reconfigure :tight @17:17:54.38 Forearm.seek 2 + 800 counts @17:17:55.39 SE.reconfigure :pick @17:17:55.45 SE.seek :beamBreak @17:17:57.16 SE.configure :default @17:17:57.34 Elbow.reconfigure :limp @17:17:57.39 Forearm.reconfigure :loose @17:17:57.44 Forearm.seek 2 @17:17:57.82 Elbow.hold @17:17:57.88 Elbow.reconfigure :tight @17:17:57.99 SE.seek 14.09 Pucks below Top Plate @17:17:58.55 Forearm.force -20 @17:17:58.60 Forearm.seek 2 + 306 counts @17:17:58.90 Forearm.reconfigure :tight @17:17:58.94 Forearm.seek 2 + 800 counts @17:17:59.23 SE.seek 15.09 Pucks below Top Plate @17:17:59.94 Forearm.reconfigure :loose @17:17:59.99 Forearm.seek :retract @17:18:00.90 Elbow.seek CC @17:18:02.52 Elbow.reconfigure :loose @17:18:02.63 Forearm.seek CC @17:18:03.58 Hand.close @17:18:04.27 CC.seek :open @17:18:08.49 Forearm.seek :retract @17:18:09.45 Elbow.reconfigure :tight @17:18:09.50 Elbow.seek 2 @17:18:11.21 Elbow.reconfigure :limp @17:18:11.26 Forearm.seek 2 @17:18:12.38 Elbow.hold @17:18:12.88 Elbow.reconfigure :tight @17:18:12.94 Hand.open @17:18:13.76 Forearm.force -20 @17:18:13.81 Forearm.seek 2 + 315 counts @17:18:14.10 Forearm.reconfigure :tight @17:18:14.15 Forearm.seek 2 + 800 counts @17:18:14.56 SE.seek :down @17:18:15.70
Forearm.reconfigure :loose @17:18:15.75 Forearm.seek :retract @17:18:15.94 SE.stop @17:18:16.66
PSV.dial :air @17:18:17.40 PS.seek 2.5ml @17:18:43.80 delay 1 second @17:18:44.81 PSV.dial :mixloop @17:18:45.25 delay 1 second @17:18:46.27 PSV.dial :air @17:18:46.72 delay 1 second @17:18:47.74 PSV.dial :mixloop @17:18:48.19 delay 1 second @17:18:49.21 PSV.dial :air @17:18:49.66 delay 1 second @17:18:50.68 PS.seek 0ml @17:19:17.02 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @17:19:17.20 Elbow.seek FlushPuck::Garage @17:19:18.98 Elbow.reconfigure :loose @17:19:19.09 Forearm.seek :garage @17:19:20.16 Hand.close @17:19:21.02 Forearm.seek :retract @17:19:22.03 Elbow.reconfigure :tight @17:19:22.08 Elbow.seek PC @17:19:23.40 Elbow.reconfigure :loose @17:19:23.51 Forearm.reconfigure :tight @17:19:23.55 Forearm.seek PC @17:19:24.79 PC.seek :closed @17:19:29.22 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.54 @17:19:29.28 Hand.open @17:19:30.08 Forearm.reconfigure :loose @17:19:30.13 Forearm.seek :retract @17:19:31.08 PRV.dial :air @17:19:31.55 PSV.dial :mixloop @17:19:32.08 PS.seek 0.3ml @17:19:36.02 PSV.dial PSR @17:19:37.05 Pulling 0.4ml of PSR.air @17:19:37.06 PSR.select :air @17:19:37.08 PS.seek 0.7ml @17:19:41.92 delay 3 seconds @17:19:44.94 PSR.select :air @17:19:44.96 PS.seek 2.5ml @17:20:04.08 delay 3 seconds @17:20:07.10 PSV.dial :mixloop @17:20:08.14 PTV.dial :mixing @17:20:08.89 PBV.dial :waste2 @17:20:09.88 PS.seek 0ml @17:20:36.24 Garaging FlushPuck in Processing Clamp @17:20:36.31 Elbow.reconfigure :tight @17:20:36.35 Elbow.seek PC @17:20:36.48 Elbow.reconfigure :loose @17:20:36.58 Forearm.seek PC @17:20:37.64 Hand.close @17:20:38.40 PC.seek :open @17:20:42.92 Forearm.seek :retract @17:20:43.93 Elbow.reconfigure :tight @17:20:43.97 Elbow.seek :garage @17:20:45.79 Elbow.reconfigure :loose @17:20:45.90 Forearm.reconfigure :tight @17:20:45.95 Forearm.seek :garage @17:20:47.06 Hand.open @17:20:47.87 Forearm.reconfigure :loose @17:20:47.92 Forearm.seek :retract @17:20:48.91 Loading da puck from tube 3 into Processing Clamp @17:20:49.15 SC.reconfigure :tight @17:20:49.20 SC.seek 3 @17:20:52.85 SC.configure :limp @17:20:53.06 Elbow.reconfigure :tight @17:20:53.11 Elbow.seek 3 @17:20:55.03 Forearm.reconfigure :tight @17:20:55.08 Forearm.seek 3 + 800 counts @17:20:56.17 SE.reconfigure :pick @17:20:56.23 SE.seek :beamBreak @17:20:58.70 SE.configure :default @17:20:58.86 Elbow.seek 3 @17:20:59.16 Forearm.seek 3 + 300 counts @17:20:59.33 Forearm.reconfigure :loose @17:20:59.38 Hand.close @17:21:00.15 SE.seek :down @17:21:00.35
Forearm.seek :retract @17:21:01.43 Elbow.seek PC @17:21:02.49 SE.stop @17:21:03.93
Elbow.reconfigure :loose @17:21:04.04 Forearm.reconfigure :tight @17:21:04.08 Forearm.seek PC @17:21:05.89 PC.seek :closed @17:21:10.31 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.56 @17:21:10.32 Hand.open @17:21:11.08 Forearm.reconfigure :loose @17:21:11.13 Forearm.seek :retract @17:21:12.21 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @17:21:12.23 PH.seek 28C,5:00 @17:22:03.40 PH.hold 2:00:00,28C @17:22:03.45 delay 1:30 @17:23:33.48 PSR.select :air @17:23:33.50 PSV.dial PSR @17:23:34.55 Pulling 0.5ml of PSR.wash @17:23:34.56 PSR.select :wash @17:23:34.67 PS.seek 0.5ml @17:23:40.64 delay 5 seconds @17:23:45.66 PSR.select :air @17:23:45.78 PS.seek 2ml @17:24:01.95 PTV.dial :puck @17:24:02.69 PSV.dial PTV @17:24:03.44 PBV.dial :waste1 @17:24:04.87 PS.seek 1.5ml @17:24:10.76 delay 3 seconds @17:24:13.78 PBV.dial between PSV and waste1 @17:24:14.16 PS.seek 0ml @17:24:30.26 delay 5:00 @17:29:30.29 recoverPSRreagent08 @17:29:30.30 PTV.dial :puck @17:29:30.37 PBV.dial :waste1 @17:29:30.76 delay 30 seconds @17:30:00.79 PBV.dial between PSV and waste1 @17:30:01.17 PSR.select :air @17:30:01.19 PSV.dial PSR @17:30:01.94 PS.reconfigure :fast @17:30:01.99 PS.seek 2.5ml @17:30:25.29 PSV.dial PTV @17:30:26.04 delay 5 seconds @17:30:31.06 PSV.dial PSR @17:30:31.81 delay 5 seconds @17:30:36.82 PSV.dial PTV @17:30:37.57 delay 5 seconds @17:30:42.59 PSV.dial :air @17:30:43.37 PS.seek 0ml @17:31:06.51 PTV.dial :PRVmixing,via: PRV @17:31:07.82 PSV.dial :mixloop @17:31:08.29 PRV.dial :ab1 @17:31:09.05 PS.reconfigure :slow @17:31:09.09 PS.seek 0.15ml @17:31:12.73 delay 2 seconds @17:31:14.75 CBV.dial PRV @17:31:14.77
PRV.dial CBV @17:31:15.81 PS.seek 0.34ml @17:31:20.20 delay 2 seconds @17:31:22.21 CBV.dial between PRV and PSV @17:31:22.55 PRV.dial :air @17:31:23.00 PS.seek 0.9ml @17:31:34.98 PRV.dial :waste2 @17:31:35.73 PS.seek 0.5ml @17:31:44.45 addPMLReagent08 @17:31:44.46 PSV.dial :air @17:31:44.92 PS.reconfigure :default @17:31:44.97 PS.seek 2ml @17:32:01.19 PSV.dial :PML @17:32:01.66 PTV.dial :mixing @17:32:02.40 PBV.dial :waste1 @17:32:02.84 PS.seek 1.85ml @17:32:05.16 PBV.dial between PSV and waste1 @17:32:05.55 PS.seek 0ml @17:32:25.35 delay 2 seconds @17:32:27.37 Beginning samplePassC @17:32:27.38 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:32:27.56 Elbow.reconfigure :tight @17:32:27.61 Elbow.seek FlushPuck::Garage @17:32:28.97 Elbow.reconfigure :loose @17:32:29.07 Forearm.seek :garage @17:32:30.12 Hand.close @17:32:31.00 Forearm.seek :retract @17:32:32.10 Elbow.reconfigure :tight @17:32:32.15 Elbow.seek CC @17:32:34.94 Elbow.reconfigure :loose @17:32:35.04 Forearm.reconfigure :tight @17:32:35.09 Forearm.seek CC @17:32:36.36 CC.seek :closed @17:32:40.35 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.05 @17:32:40.43 Hand.open @17:32:41.21 Forearm.reconfigure :loose @17:32:41.25 Forearm.seek :retract @17:32:42.27 PSV.dial PSR @17:32:43.32 Pulling 1.5ml of PSR.flush @17:32:43.33 PSR.select :flush @17:32:43.45 PS.seek 1.5ml @17:32:59.57 delay 5 seconds @17:33:04.59 PSV.dial :waste1 @17:33:05.06 PS.seek 0ml @17:33:21.19 PSV.dial PSR @17:33:21.66 PSR.select :air @17:33:21.78 PS.seek 2.5ml @17:33:48.17 PRV.dial CBV,avoiding: :ab2 @17:33:48.20
CBV.dial PRV @17:33:49.82 PSV.dial :mixloop @17:33:50.87 CTV.dial :air,avoiding: :intake @17:33:50.95 PTV.dial :PRVmixing @17:33:51.70 PS.seek 0ml @17:34:18.07 PSV.dial :air @17:34:18.53 PS.seek 2.5ml @17:34:44.94 PSV.dial :mixloop @17:34:45.42 PS.seek 0ml @17:35:11.72 PSV.dial :air @17:35:12.20 PS.seek 2.5ml @17:35:38.53 PSV.dial :mixloop @17:35:39.01 PS.seek 0ml @17:36:05.35 Garaging FlushPuck in Collection Clamp @17:36:05.42 Elbow.reconfigure :tight @17:36:05.47 Elbow.seek CC @17:36:05.59 Elbow.reconfigure :loose @17:36:05.70 Forearm.seek CC @17:36:06.72 Hand.close @17:36:07.51 CC.seek :open @17:36:12.05 Forearm.seek :retract @17:36:13.15 Elbow.reconfigure :tight @17:36:13.20 Elbow.seek :garage @17:36:16.06 Elbow.reconfigure :loose @17:36:16.16 Forearm.reconfigure :tight @17:36:16.21 Forearm.seek :garage @17:36:17.22 Hand.open @17:36:18.07 Forearm.reconfigure :loose @17:36:18.11 Forearm.seek :retract @17:36:19.21 delayUntil "17:40:27.36" @17:36:19.23 Whole Cell Archive sampling 1000ml @17:36:19.25 Loading wcr puck from tube 3 into Collection Clamp @17:36:20.14 Elbow.reconfigure :tight @17:36:20.20 Elbow.seek 3 @17:36:22.08 Forearm.reconfigure :tight @17:36:22.14 Forearm.seek 3 + 800 counts @17:36:23.22 SE.reconfigure :pick @17:36:23.29 SE.seek :beamBreak @17:36:25.93 SE.configure :default @17:36:26.10 Elbow.seek 3 @17:36:26.29 Forearm.seek 3 + 300 counts @17:36:26.47 Forearm.reconfigure :loose @17:36:26.52 Hand.close @17:36:27.26 SE.seek :down @17:36:27.47 Forearm.seek :retract @17:36:28.46 Elbow.seek CC @17:36:29.73 SE.stop @17:36:30.48 Elbow.reconfigure :loose @17:36:30.60 Forearm.reconfigure :tight @17:36:30.65 Forearm.seek CC @17:36:31.99 CC.seek :closed @17:36:35.96 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.90 @17:36:35.97 Hand.open @17:36:36.81 Forearm.reconfigure :loose @17:36:36.86 Forearm.seek :retract @17:36:37.89 collectSample 1000ml w/bubble point of 35psi @17:36:37.91 Priming 7ml @17:36:37.92 Intake.select :close @17:36:37.93 Exhaust.select :close @17:36:37.94 SS.hold @17:36:38.18 Calibrating pressure sensors @17:36:38.19 CTV.dial :air @17:36:38.21 SSV.dial :spit,avoiding: :exhaust @17:36:38.29 SS.reconfigure :fast @17:36:38.34 SS.seek 5ml @17:36:41.05 SSV.dial CBV @17:36:41.08 CBV.dial SSV @17:36:42.45 delay 3.8 seconds @17:36:46.38 Sensor limits: Intake max 102.6psia; Outlet max 110.2psia @17:36:46.40 SSV.dial :spit @17:36:47.76 SS.seek 0ml @17:36:50.31 SSV.dial CBV @17:36:51.68 CTV.dial :intake @17:36:52.47 SSV.dial CBV @17:36:52.49 CBV.dial SSV @17:36:52.64 Allowing 40s for 7.0ml of remaining 7.0ml @17:36:52.65 Intake.select :open @17:36:55.25 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:36:55.29 SS.reconfigure :inhaling 2.17ml/s @17:36:55.34 SS.seek 7ml @17:37:01.75 Stroke completed -- waiting for pressure to equalize ... @17:37:01.76 SS.reconfigure :exhale @17:37:01.81 SS.hold @17:37:12.98 Intake.select :close @17:37:13.14 SSV.dial :exhaust @17:37:13.94 Exhaust.select :open @17:37:14.05 SS.seek 0ml @17:37:18.05 Exhaust.select :close @17:37:18.17 Sampling 1000.0ml @17:37:18.18 Intake.select :close @17:37:18.19 Exhaust.select :close @17:37:18.20 SS.reconfigure :default @17:37:18.25 SS.hold @17:37:18.46 CTV.dial :intake @17:37:18.55 SSV.dial CBV @17:37:18.58 CBV.dial SSV @17:37:19.83 Allowing 130s for 25.0ml of remaining 1000.0ml @17:37:19.84 Intake.select :open @17:37:22.01 Intake @14.2psia, vacuum from 8.7 to 11.7psi @17:37:22.03 SS.reconfigure :inhaling 2.17ml/s @17:37:22.08 SS.seek 25ml @17:37:35.11 Vacuum too high -- waiting up to 40 seconds @17:37:35.13 SS.reconfigure :exhale @17:37:35.19 SS.hold @17:37:35.87 Intake @11.4psia, vacuum from 5.9 to 8.9psi @17:37:35.89 SS.reconfigure :inhaling 1.95ml/s @17:37:35.94 SS.seek 25ml @17:37:36.02 Vacuum too high -- waiting up to 40 seconds @17:37:36.03 SS.reconfigure :exhale @17:37:36.09 SS.hold @17:37:47.34 Intake @12.1psia, vacuum from 6.6 to 9.6psi @17:37:47.36 SS.reconfigure :inhaling 1.77ml/s @17:37:47.41 SS.seek 25ml @17:37:50.04 Stroke completed -- waiting for pressure to equalize ... @17:37:50.05 SS.reconfigure :exhale @17:37:50.52 SS.hold @17:37:53.41 Intake.select :close @17:37:53.57 SSV.dial :exhaust @17:37:54.34 Exhaust.select :open @17:37:54.45 SS.seek 0ml @17:38:03.68 Exhaust.select :close @17:38:03.80 SSV.dial CBV @17:38:03.83 CBV.dial SSV @17:38:04.61 Allowing 130s for 25.0ml of remaining 975.0ml @17:38:04.62 Intake.select :open @17:38:06.79 Intake @13.9psia, vacuum from 8.4 to 11.4psi @17:38:06.81 SS.reconfigure :inhaling 1.77ml/s @17:38:06.85 SS.seek 25ml @17:38:17.21 Vacuum too high -- waiting up to 40 seconds @17:38:17.22 SS.reconfigure :exhale @17:38:17.28 SS.hold @17:38:19.18 Intake @11.3psia, vacuum from 5.8 to 8.8psi @17:38:19.20 SS.reconfigure :inhaling 1.59ml/s @17:38:19.25 SS.seek 25ml @17:38:21.33 Vacuum too high -- waiting up to 40 seconds @17:38:21.35 SS.reconfigure :exhale @17:38:21.41 SS.hold @17:38:26.09 Intake @12.5psia, vacuum from 7.0 to 10.0psi @17:38:26.11 SS.reconfigure :inhaling 1.43ml/s @17:38:26.16 SS.seek 25ml @17:38:32.28 Stroke completed -- waiting for pressure to equalize ... @17:38:32.29 SS.reconfigure :exhale @17:38:32.34 SS.hold @17:38:37.10 Intake.select :close @17:38:37.26 SSV.dial :exhaust @17:38:38.03 Exhaust.select :open @17:38:38.15 SS.seek 0ml @17:38:47.34 Exhaust.select :close @17:38:47.45 SSV.dial CBV @17:38:47.47 CBV.dial SSV @17:38:48.26 Allowing 130s for 25.0ml of remaining 950.0ml @17:38:48.28 Intake.select :open @17:38:50.90 Intake @13.5psia, vacuum from 8.0 to 11.0psi @17:38:50.92 SS.reconfigure :inhaling 1.43ml/s @17:38:50.97 SS.seek 25ml @17:39:07.52 Vacuum too high -- waiting up to 40 seconds @17:39:07.54 SS.reconfigure :exhale @17:39:07.59 SS.hold @17:39:11.32 Intake @11.6psia, vacuum from 6.1 to 9.1psi @17:39:11.34 SS.reconfigure :inhaling 1.29ml/s @17:39:11.39 SS.seek 25ml @17:39:15.26 Stroke completed -- waiting for pressure to equalize ... @17:39:15.27 SS.reconfigure :exhale @17:39:15.32 SS.hold @17:39:21.43 Intake.select :close @17:39:21.59 SSV.dial :exhaust @17:39:22.35 Exhaust.select :open @17:39:22.47 SS.seek 0ml @17:39:31.34 Exhaust.select :close @17:39:31.45 SSV.dial CBV @17:39:31.48 CBV.dial SSV @17:39:32.29 Allowing 130s for 25.0ml of remaining 925.0ml @17:39:32.31 Intake.select :open @17:39:34.48 Intake @13.9psia, vacuum from 8.4 to 11.4psi @17:39:34.50 SS.reconfigure :inhaling 1.29ml/s @17:39:34.55 SS.seek 25ml @17:39:51.70 Vacuum too high -- waiting up to 40 seconds @17:39:51.72 SS.reconfigure :exhale @17:39:51.78 SS.hold @17:39:53.17 Intake @11.4psia, vacuum from 5.9 to 8.9psi @17:39:53.19 SS.reconfigure :inhaling 1.16ml/s @17:39:53.24 SS.seek 25ml @17:39:58.46 Stroke completed -- waiting for pressure to equalize ... @17:39:58.47 SS.reconfigure :exhale @17:39:58.52 SS.hold @17:40:05.47 Intake.select :close @17:40:05.64 SSV.dial :exhaust @17:40:06.40 Exhaust.select :open @17:40:06.52 SS.seek 0ml @17:40:15.84 Exhaust.select :close @17:40:15.95 SSV.dial CBV @17:40:15.98 CBV.dial SSV @17:40:16.78 Allowing 130s for 25.0ml of remaining 900.0ml @17:40:16.79 Intake.select :open @17:40:18.96 Intake @14.0psia, vacuum from 8.5 to 11.5psi @17:40:18.98 SS.reconfigure :inhaling 1.16ml/s @17:40:19.02 SS.seek 25ml @17:40:27.37 recoverPRVreagent08 @17:40:27.38 PTV.dial :mixing @17:40:28.13 PBV.dial :waste1 @17:40:28.52 delay 30 seconds @17:40:36.99 Vacuum too high -- waiting up to 40 seconds @17:40:37.00 SS.reconfigure :exhale @17:40:37.06 SS.hold @17:40:38.41 Intake @11.7psia, vacuum from 6.2 to 9.2psi @17:40:38.43 SS.reconfigure :inhaling 1.04ml/s @17:40:38.48 SS.seek 25ml @17:40:45.02 Stroke completed -- waiting for pressure to equalize ... @17:40:45.03 SS.reconfigure :exhale @17:40:45.08 SS.hold @17:40:50.76 Intake.select :close @17:40:50.92 SSV.dial :exhaust @17:40:51.68 Exhaust.select :open @17:40:51.80 SS.seek 0ml @17:40:58.54 PBV.dial between PSV and waste1 @17:40:58.94 PSV.dial :air @17:40:59.41 PS.reconfigure :fast @17:40:59.47 PS.seek 2.5ml @17:41:00.96 Exhaust.select :close @17:41:01.07 SSV.dial CBV @17:41:01.10 CBV.dial SSV @17:41:01.88 Allowing 130s for 25.0ml of remaining 875.0ml @17:41:01.90 Intake.select :open @17:41:04.07 Intake @13.7psia, vacuum from 8.2 to 11.2psi @17:41:04.09 SS.reconfigure :inhaling 1.04ml/s @17:41:04.14 SS.seek 25ml @17:41:23.19 PSV.dial :mixloop @17:41:23.66 delay 2 seconds @17:41:25.68 PSV.dial :air @17:41:26.14 delay 2 seconds @17:41:28.16 PSV.dial :mixloop @17:41:28.63 delay 2 seconds @17:41:29.93 Stroke completed -- waiting for pressure to equalize ... @17:41:29.94 SS.reconfigure :exhale @17:41:29.99 SS.hold @17:41:30.64 PSV.dial :air @17:41:31.11 PS.seek 0ml @17:41:37.52 Intake.select :close @17:41:37.69 SSV.dial :exhaust @17:41:38.46 Exhaust.select :open @17:41:38.59 SS.seek 0ml @17:41:47.84 Exhaust.select :close @17:41:47.95 SSV.dial CBV @17:41:47.99 CBV.dial SSV @17:41:48.77 Allowing 130s for 25.0ml of remaining 850.0ml @17:41:48.79 Intake.select :open @17:41:50.96 Intake @14.2psia, vacuum from 8.7 to 11.7psi @17:41:50.98 SS.reconfigure :inhaling 1.04ml/s @17:41:51.03 SS.seek 25ml @17:41:54.50 washCycleDA #1 of 2 @17:41:54.51 PSR.select :air @17:41:54.53 PSV.dial PSR @17:41:55.86 Pulling 0.5ml of PSR.wash @17:41:55.87 PSR.select :wash @17:41:55.99 PS.reconfigure :default @17:41:56.04 PS.seek 0.5ml @17:42:01.94 delay 5 seconds @17:42:06.96 PSR.select :air @17:42:07.08 PS.seek 2ml @17:42:12.87 Vacuum too high -- waiting up to 40 seconds @17:42:12.89 SS.reconfigure :exhale @17:42:12.94 SS.hold @17:42:13.38 Intake @11.6psia, vacuum from 6.1 to 9.1psi @17:42:13.40 SS.reconfigure :inhaling 0.94ml/s @17:42:13.45 SS.seek 25ml @17:42:18.03 Stroke completed -- waiting for pressure to equalize ... @17:42:18.04 SS.reconfigure :exhale @17:42:18.09 SS.hold @17:42:23.22 PTV.dial :puck @17:42:23.97 PSV.dial PTV @17:42:24.68 Intake.select :close @17:42:24.75 PBV.dial :waste1 @17:42:24.86 SSV.dial :exhaust @17:42:25.20 PS.seek 1.5ml @17:42:25.63 Exhaust.select :open @17:42:25.75 SS.seek 0ml @17:42:31.09 delay 3 seconds @17:42:34.11 PBV.dial between PSV and waste1 @17:42:34.51 PS.seek 0ml @17:42:34.93 Exhaust.select :close @17:42:35.04 SSV.dial CBV @17:42:35.07 CBV.dial SSV @17:42:35.87 Allowing 130s for 25.0ml of remaining 825.0ml @17:42:35.88 Intake.select :open @17:42:38.05 Intake @14.0psia, vacuum from 8.5 to 11.5psi @17:42:38.06 SS.reconfigure :inhaling 0.94ml/s @17:42:38.11 SS.seek 25ml @17:42:50.81 delay 2:00 @17:43:06.32 Stroke completed -- waiting for pressure to equalize ... @17:43:06.33 SS.reconfigure :exhale @17:43:06.38 SS.hold @17:43:13.87 Intake.select :close @17:43:14.04 SSV.dial :exhaust @17:43:14.80 Exhaust.select :open @17:43:14.92 SS.seek 0ml @17:43:24.38 Exhaust.select :close @17:43:24.49 SSV.dial CBV @17:43:24.53 CBV.dial SSV @17:43:25.31 Allowing 130s for 25.0ml of remaining 800.0ml @17:43:25.32 Intake.select :open @17:43:27.49 Intake @14.4psia, vacuum from 8.9 to 11.9psi @17:43:27.51 SS.reconfigure :inhaling 0.94ml/s @17:43:27.55 SS.seek 25ml @17:43:55.72 Stroke completed -- waiting for pressure to equalize ... @17:43:55.73 SS.reconfigure :exhale @17:43:55.78 SS.hold @17:44:04.39 Intake.select :close @17:44:04.56 SSV.dial :exhaust @17:44:05.32 Exhaust.select :open @17:44:05.44 SS.seek 0ml @17:44:14.71 Exhaust.select :close @17:44:14.82 SSV.dial CBV @17:44:14.85 CBV.dial SSV @17:44:15.65 Allowing 130s for 25.0ml of remaining 775.0ml @17:44:15.66 Intake.select :open @17:44:17.83 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:44:17.84 SS.reconfigure :inhaling 0.94ml/s @17:44:17.89 SS.seek 25ml @17:44:45.91 Stroke completed -- waiting for pressure to equalize ... @17:44:45.92 SS.reconfigure :exhale @17:44:45.97 SS.hold @17:44:50.85 recoverPSRreagent08 @17:44:50.86 PTV.dial :puck @17:44:50.94 PBV.dial :waste1 @17:44:51.33 delay 30 seconds @17:44:57.12 Intake.select :close @17:44:57.28 SSV.dial :exhaust @17:44:58.05 Exhaust.select :open @17:44:58.17 SS.seek 0ml @17:45:07.49 Exhaust.select :close @17:45:07.60 SSV.dial CBV @17:45:07.63 CBV.dial SSV @17:45:08.43 Allowing 130s for 25.0ml of remaining 750.0ml @17:45:08.44 Intake.select :open @17:45:10.61 Intake @14.4psia, vacuum from 8.9 to 11.9psi @17:45:10.63 SS.reconfigure :inhaling 0.94ml/s @17:45:10.67 SS.seek 25ml @17:45:21.35 PBV.dial between PSV and waste1 @17:45:21.73 PSR.select :air @17:45:21.75 PSV.dial PSR @17:45:22.52 PS.reconfigure :fast @17:45:22.57 PS.seek 2.5ml @17:45:38.77 Stroke completed -- waiting for pressure to equalize ... @17:45:38.78 SS.reconfigure :exhale @17:45:38.83 SS.hold @17:45:46.18 PSV.dial PTV @17:45:46.95 delay 5 seconds @17:45:48.65 Intake.select :close @17:45:48.82 SSV.dial :exhaust @17:45:49.58 Exhaust.select :open @17:45:49.70 SS.seek 0ml @17:45:51.97 PSV.dial PSR @17:45:52.72 delay 5 seconds @17:45:57.74 PSV.dial PTV @17:45:58.48 delay 5 seconds @17:45:59.08 Exhaust.select :close @17:45:59.19 SSV.dial CBV @17:45:59.23 CBV.dial SSV @17:46:00.00 Allowing 130s for 25.0ml of remaining 725.0ml @17:46:00.02 Intake.select :open @17:46:02.19 Intake @14.6psia, vacuum from 9.1 to 12.1psi @17:46:02.21 SS.reconfigure :inhaling 0.94ml/s @17:46:02.26 SS.seek 25ml @17:46:03.50 PSV.dial :air @17:46:04.27 PS.seek 0ml @17:46:26.17 Vacuum too high -- waiting up to 40 seconds @17:46:26.18 SS.reconfigure :exhale @17:46:26.24 SS.hold @17:46:27.54 washCycleDA #2 of 2 @17:46:27.56 PSR.select :air @17:46:27.59 PSV.dial PSR @17:46:28.92 Pulling 0.5ml of PSR.wash @17:46:28.93 PSR.select :wash @17:46:29.05 PS.reconfigure :default @17:46:29.10 PS.seek 0.5ml @17:46:30.51 Intake @13.0psia, vacuum from 7.5 to 10.5psi @17:46:30.53 SS.reconfigure :inhaling 0.85ml/s @17:46:31.04 SS.seek 25ml @17:46:35.06 delay 5 seconds @17:46:35.94 Stroke completed -- waiting for pressure to equalize ... @17:46:35.95 SS.reconfigure :exhale @17:46:36.00 SS.hold @17:46:40.14 PSR.select :air @17:46:40.34 PS.seek 2ml @17:46:43.50 Intake.select :close @17:46:43.67 SSV.dial :exhaust @17:46:44.44 Exhaust.select :open @17:46:44.56 SS.seek 0ml @17:46:53.92 Exhaust.select :close @17:46:54.04 SSV.dial CBV @17:46:54.07 CBV.dial SSV @17:46:54.85 Allowing 130s for 25.0ml of remaining 700.0ml @17:46:54.86 Intake.select :open @17:46:56.53 PTV.dial :puck @17:46:56.54 PSV.dial PTV @17:46:57.03 Intake @14.3psia, vacuum from 8.8 to 11.8psi @17:46:57.05 SS.reconfigure :inhaling 0.85ml/s @17:46:57.10 SS.seek 25ml @17:46:57.29 PBV.dial :waste1 @17:46:57.73 PS.seek 1.5ml @17:47:03.72 delay 3 seconds @17:47:06.73 PBV.dial between PSV and waste1 @17:47:07.58 PS.seek 0ml @17:47:23.78 delay 2:00 @17:47:27.78 Stroke completed -- waiting for pressure to equalize ... @17:47:27.79 SS.reconfigure :exhale @17:47:27.84 SS.hold @17:47:40.52 Intake.select :close @17:47:40.68 SSV.dial :exhaust @17:47:41.45 Exhaust.select :open @17:47:41.58 SS.seek 0ml @17:47:50.55 Exhaust.select :close @17:47:50.66 SSV.dial CBV @17:47:50.70 CBV.dial SSV @17:47:51.47 Allowing 130s for 25.0ml of remaining 675.0ml @17:47:51.49 Intake.select :open @17:47:53.66 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:47:53.67 SS.reconfigure :inhaling 0.85ml/s @17:47:53.72 SS.seek 25ml @17:48:18.04 Vacuum too high -- waiting up to 40 seconds @17:48:18.06 SS.reconfigure :exhale @17:48:18.11 SS.hold @17:48:22.39 Intake @13.7psia, vacuum from 8.2 to 11.2psi @17:48:22.41 SS.reconfigure :inhaling 0.77ml/s @17:48:22.46 SS.seek 25ml @17:48:30.72 Stroke completed -- waiting for pressure to equalize ... @17:48:30.73 SS.reconfigure :exhale @17:48:30.78 SS.hold @17:48:39.24 Intake.select :close @17:48:39.41 SSV.dial :exhaust @17:48:40.17 Exhaust.select :open @17:48:40.29 SS.seek 0ml @17:48:49.59 Exhaust.select :close @17:48:49.71 SSV.dial CBV @17:48:49.74 CBV.dial SSV @17:48:50.52 Allowing 130s for 25.0ml of remaining 650.0ml @17:48:50.53 Intake.select :open @17:48:52.70 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:48:52.72 SS.reconfigure :inhaling 0.77ml/s @17:48:52.77 SS.seek 25ml @17:49:23.81 recoverPSRreagent08 @17:49:23.82 PTV.dial :puck @17:49:23.83 PBV.dial :waste1 @17:49:24.22 delay 30 seconds @17:49:26.65 Stroke completed -- waiting for pressure to equalize ... @17:49:26.67 SS.reconfigure :exhale @17:49:26.72 SS.hold @17:49:37.90 Intake.select :close @17:49:38.06 SSV.dial :exhaust @17:49:38.83 Exhaust.select :open @17:49:38.95 SS.seek 0ml @17:49:47.86 Exhaust.select :close @17:49:47.97 SSV.dial CBV @17:49:48.00 CBV.dial SSV @17:49:48.81 Allowing 130s for 25.0ml of remaining 625.0ml @17:49:48.83 Intake.select :open @17:49:51.00 Intake @15.1psia, vacuum from 9.6 to 12.6psi @17:49:51.01 SS.reconfigure :inhaling 0.77ml/s @17:49:51.06 SS.seek 25ml @17:49:54.23 PBV.dial between PSV and waste1 @17:49:54.60 PSR.select :air @17:49:54.62 PSV.dial PSR @17:49:55.39 PS.reconfigure :fast @17:49:55.44 PS.seek 2.5ml @17:50:18.99 PSV.dial PTV @17:50:19.76 delay 5 seconds @17:50:21.44 Vacuum too high -- waiting up to 40 seconds @17:50:21.46 SS.reconfigure :exhale @17:50:21.52 SS.hold @17:50:24.78 PSV.dial PSR @17:50:25.53 delay 5 seconds @17:50:25.80 Intake @13.5psia, vacuum from 8.0 to 11.0psi @17:50:25.82 SS.reconfigure :inhaling 0.70ml/s @17:50:25.87 SS.seek 25ml @17:50:30.40 Stroke completed -- waiting for pressure to equalize ... @17:50:30.41 SS.reconfigure :exhale @17:50:30.46 SS.hold @17:50:31.00 PSV.dial PTV @17:50:31.76 delay 5 seconds @17:50:36.78 PSV.dial :air @17:50:37.55 PS.seek 0ml @17:50:40.61 Intake.select :close @17:50:40.77 SSV.dial :exhaust @17:50:41.53 Exhaust.select :open @17:50:41.65 SS.seek 0ml @17:50:50.95 Exhaust.select :close @17:50:51.06 SSV.dial CBV @17:50:51.09 CBV.dial SSV @17:50:51.87 Allowing 130s for 25.0ml of remaining 600.0ml @17:50:51.89 Intake.select :open @17:50:54.06 Intake @15.3psia, vacuum from 9.8 to 12.8psi @17:50:54.08 SS.reconfigure :inhaling 0.70ml/s @17:50:54.12 SS.seek 25ml @17:51:01.15 getPMLReagent08 @17:51:01.16 PRV.dial :air @17:51:01.62 delay 5 seconds @17:51:06.64 PTV.dial :PRVmixing @17:51:07.99 delay 5 seconds @17:51:13.02 PRV.dial :ab2 @17:51:13.93 PTV.dial :PRVmixing @17:51:14.01 PSV.dial :mixloop @17:51:14.48 PS.reconfigure :default @17:51:14.53 PS.seek 0.3ml @17:51:18.41 delay 2 seconds @17:51:20.43 PRV.dial :air @17:51:20.96 PS.seek 0.55ml @17:51:24.31 addPMLReagent08 @17:51:24.32 PSV.dial :air @17:51:24.80 PS.seek 2ml @17:51:31.32 Stroke completed -- waiting for pressure to equalize ... @17:51:31.34 SS.reconfigure :exhale @17:51:31.39 SS.hold @17:51:40.49 PSV.dial :PML @17:51:40.96 PTV.dial :mixing @17:51:41.71 PBV.dial :waste1 @17:51:42.16 PS.seek 1.85ml @17:51:44.86 PBV.dial between PSV and waste1 @17:51:45.31 PS.seek 0ml @17:51:45.77 Intake.select :close @17:51:45.94 SSV.dial :exhaust @17:51:46.71 Exhaust.select :open @17:51:46.84 SS.seek 0ml @17:51:56.26 Exhaust.select :close @17:51:56.37 SSV.dial CBV @17:51:56.40 CBV.dial SSV @17:51:57.18 Allowing 130s for 25.0ml of remaining 575.0ml @17:51:57.20 Intake.select :open @17:51:59.37 Intake @14.7psia, vacuum from 9.2 to 12.2psi @17:51:59.39 SS.reconfigure :inhaling 0.70ml/s @17:51:59.44 SS.seek 25ml @17:52:05.05 delay 2 seconds @17:52:07.07 delay 8:00 @17:52:29.91 Vacuum too high -- waiting up to 40 seconds @17:52:29.93 SS.reconfigure :exhale @17:52:29.99 SS.hold @17:52:35.75 Intake @13.9psia, vacuum from 8.4 to 11.4psi @17:52:35.77 SS.reconfigure :inhaling 0.62ml/s @17:52:35.82 SS.seek 25ml @17:52:43.82 Stroke completed -- waiting for pressure to equalize ... @17:52:43.83 SS.reconfigure :exhale @17:52:43.88 SS.hold @17:52:54.03 Intake.select :close @17:52:54.19 SSV.dial :exhaust @17:52:54.95 Exhaust.select :open @17:52:55.06 SS.seek 0ml @17:53:04.43 Exhaust.select :close @17:53:04.54 SSV.dial CBV @17:53:04.56 CBV.dial SSV @17:53:05.35 Allowing 130s for 25.0ml of remaining 550.0ml @17:53:05.37 Intake.select :open @17:53:07.54 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:53:07.56 SS.reconfigure :inhaling 0.62ml/s @17:53:07.61 SS.seek 25ml @17:53:40.56 Vacuum too high -- waiting up to 40 seconds @17:53:40.58 SS.reconfigure :exhale @17:53:40.64 SS.hold @17:53:45.13 Intake @14.0psia, vacuum from 8.5 to 11.5psi @17:53:45.15 SS.reconfigure :inhaling 0.56ml/s @17:53:45.20 SS.seek 25ml @17:53:54.80 Stroke completed -- waiting for pressure to equalize ... @17:53:54.82 SS.reconfigure :exhale @17:53:54.87 SS.hold @17:54:06.01 Intake.select :close @17:54:06.58 SSV.dial :exhaust @17:54:07.42 Exhaust.select :open @17:54:07.54 SS.seek 0ml @17:54:17.01 Exhaust.select :close @17:54:17.12 SSV.dial CBV @17:54:17.15 CBV.dial SSV @17:54:17.99 Allowing 130s for 25.0ml of remaining 525.0ml @17:54:18.01 Intake.select :open @17:54:20.18 Intake @15.4psia, vacuum from 9.9 to 12.9psi @17:54:20.20 SS.reconfigure :inhaling 0.56ml/s @17:54:20.25 SS.seek 25ml @17:55:03.78 Vacuum too high -- waiting up to 40 seconds @17:55:03.80 SS.reconfigure :exhale @17:55:03.86 SS.hold @17:55:10.48 Intake @14.2psia, vacuum from 8.7 to 11.7psi @17:55:10.50 SS.reconfigure :inhaling 0.51ml/s @17:55:10.54 SS.seek 25ml @17:55:13.02 Stroke completed -- waiting for pressure to equalize ... @17:55:13.03 SS.reconfigure :exhale @17:55:13.08 SS.hold @17:55:24.46 Intake.select :close @17:55:24.62 SSV.dial :exhaust @17:55:25.38 Exhaust.select :open @17:55:25.50 SS.seek 0ml @17:55:34.94 Exhaust.select :close @17:55:35.06 SSV.dial CBV @17:55:35.09 CBV.dial SSV @17:55:35.87 Allowing 130s for 25.0ml of remaining 500.0ml @17:55:35.88 Intake.select :open @17:55:38.05 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:55:38.06 SS.reconfigure :inhaling 0.51ml/s @17:55:38.11 SS.seek 25ml @17:56:28.69 Stroke completed -- waiting for pressure to equalize ... @17:56:28.70 SS.reconfigure :exhale @17:56:28.75 SS.hold @17:56:51.73 Intake.select :close @17:56:51.89 SSV.dial :exhaust @17:56:52.66 Exhaust.select :open @17:56:52.78 SS.seek 0ml @17:57:02.19 Exhaust.select :close @17:57:02.30 SSV.dial CBV @17:57:02.34 CBV.dial SSV @17:57:03.12 Allowing 130s for 25.0ml of remaining 475.0ml @17:57:03.13 Intake.select :open @17:57:05.30 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:57:05.32 SS.reconfigure :inhaling 0.51ml/s @17:57:05.37 SS.seek 25ml @17:57:41.15 Vacuum too high -- waiting up to 40 seconds @17:57:41.17 SS.reconfigure :exhale @17:57:41.22 SS.hold @17:57:47.17 Intake @14.6psia, vacuum from 9.1 to 12.1psi @17:57:47.19 SS.reconfigure :inhaling 0.46ml/s @17:57:47.24 SS.seek 25ml @17:58:03.74 Stroke completed -- waiting for pressure to equalize ... @17:58:03.75 SS.reconfigure :exhale @17:58:03.80 SS.hold @17:58:22.22 Intake.select :close @17:58:22.38 SSV.dial :exhaust @17:58:23.14 Exhaust.select :open @17:58:23.26 SS.seek 0ml @17:58:32.74 Exhaust.select :close @17:58:32.85 SSV.dial CBV @17:58:32.89 CBV.dial SSV @17:58:33.66 Allowing 130s for 25.0ml of remaining 450.0ml @17:58:33.68 Intake.select :open @17:58:35.84 Intake @16.0psia, vacuum from 10.0 to 13.0psi @17:58:35.86 SS.reconfigure :inhaling 0.46ml/s @17:58:35.91 SS.seek 25ml @17:59:21.77 Vacuum too high -- waiting up to 40 seconds @17:59:21.79 SS.reconfigure :exhale @17:59:21.85 SS.hold @17:59:32.27 Intake @15.8psia, vacuum from 10.0 to 13.0psi @17:59:32.29 SS.reconfigure :inhaling 0.42ml/s @17:59:32.34 SS.seek 25ml @17:59:43.00 Stroke completed -- waiting for pressure to equalize ... @17:59:43.01 SS.reconfigure :exhale @17:59:43.07 SS.hold @18:00:05.53 Intake.select :close @18:00:05.70 SSV.dial :exhaust @18:00:06.47 Exhaust.select :open @18:00:06.59 SS.seek 0ml @18:00:07.09 recoverPRVreagent08 @18:00:07.10 PTV.dial :mixing @18:00:07.17 PBV.dial :waste1 @18:00:07.57 delay 30 seconds @18:00:15.94 Exhaust.select :close @18:00:16.05 SSV.dial CBV @18:00:16.10 CBV.dial SSV @18:00:16.86 Allowing 130s for 25.0ml of remaining 425.0ml @18:00:16.88 Intake.select :open @18:00:19.04 Intake @16.1psia, vacuum from 10.0 to 13.0psi @18:00:19.07 SS.reconfigure :inhaling 0.42ml/s @18:00:19.12 SS.seek 25ml @18:00:37.58 PBV.dial between PSV and waste1 @18:00:37.97 PSV.dial :air @18:00:38.57 PS.reconfigure :fast @18:00:38.62 PS.seek 2.5ml @18:01:00.18 Vacuum too high -- waiting up to 40 seconds @18:01:00.20 SS.reconfigure :exhale @18:01:00.26 SS.hold @18:01:02.19 PSV.dial :mixloop @18:01:02.66 delay 2 seconds @18:01:04.73 PSV.dial :air @18:01:05.21 delay 2 seconds @18:01:07.23 PSV.dial :mixloop @18:01:07.63 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:01:07.65 SS.reconfigure :inhaling 0.38ml/s @18:01:07.73 SS.seek 25ml @18:01:07.75 delay 2 seconds @18:01:09.77 PSV.dial :air @18:01:10.25 PS.seek 0ml @18:01:30.65 Stroke completed -- waiting for pressure to equalize ... @18:01:30.67 SS.reconfigure :exhale @18:01:30.72 SS.hold @18:01:33.67 washCycleDA #1 of 3 @18:01:33.69 PSR.select :air @18:01:33.74 PSV.dial PSR @18:01:35.11 Pulling 0.5ml of PSR.wash @18:01:35.12 PSR.select :wash @18:01:35.24 PS.reconfigure :default @18:01:35.29 PS.seek 0.5ml @18:01:41.24 delay 5 seconds @18:01:46.26 PSR.select :air @18:01:46.39 PS.seek 2ml @18:01:53.27 Intake.select :close @18:01:53.44 SSV.dial :exhaust @18:01:54.20 Exhaust.select :open @18:01:54.32 SS.seek 0ml @18:02:02.61 PTV.dial :puck @18:02:03.35 Exhaust.select :close @18:02:03.42 PSV.dial PTV @18:02:03.47 SSV.dial CBV @18:02:03.50 CBV.dial SSV @18:02:04.17 PBV.dial :waste1 @18:02:04.31 Allowing 130s for 25.0ml of remaining 400.0ml @18:02:04.33 Intake.select :open @18:02:04.63 PS.seek 1.5ml @18:02:06.50 Intake @16.2psia, vacuum from 10.0 to 13.0psi @18:02:06.52 SS.reconfigure :inhaling 0.38ml/s @18:02:06.56 SS.seek 25ml @18:02:10.61 delay 3 seconds @18:02:13.63 PBV.dial between PSV and waste1 @18:02:14.02 PS.seek 0ml @18:02:30.11 delay 2:00 @18:02:52.53 Vacuum too high -- waiting up to 40 seconds @18:02:52.54 SS.reconfigure :exhale @18:02:52.60 SS.hold @18:03:00.67 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:03:00.69 SS.reconfigure :inhaling 0.33ml/s @18:03:00.74 SS.seek 25ml @18:03:26.60 Stroke completed -- waiting for pressure to equalize ... @18:03:26.62 SS.reconfigure :exhale @18:03:26.67 SS.hold @18:03:51.09 Intake.select :close @18:03:51.25 SSV.dial :exhaust @18:03:52.01 Exhaust.select :open @18:03:52.13 SS.seek 0ml @18:04:01.64 Exhaust.select :close @18:04:01.75 SSV.dial CBV @18:04:01.79 CBV.dial SSV @18:04:02.56 Allowing 130s for 25.0ml of remaining 375.0ml @18:04:02.57 Intake.select :open @18:04:04.75 Intake @16.3psia, vacuum from 10.0 to 13.0psi @18:04:04.77 SS.reconfigure :inhaling 0.33ml/s @18:04:04.82 SS.seek 25ml @18:04:30.13 recoverPSRreagent08 @18:04:30.14 PTV.dial :puck @18:04:30.22 PBV.dial :waste1 @18:04:30.59 delay 30 seconds @18:05:00.62 PBV.dial between PSV and waste1 @18:05:01.53 PSR.select :air @18:05:01.55 PSV.dial PSR @18:05:02.43 PS.reconfigure :fast @18:05:02.48 PS.seek 2.5ml @18:05:07.99 Vacuum too high -- waiting up to 40 seconds @18:05:08.01 SS.reconfigure :exhale @18:05:08.06 SS.hold @18:05:15.66 Intake @15.6psia, vacuum from 10.0 to 13.0psi @18:05:15.68 SS.reconfigure :inhaling 0.30ml/s @18:05:15.73 SS.seek 25ml @18:05:25.98 PSV.dial PTV @18:05:26.72 delay 5 seconds @18:05:31.74 PSV.dial PSR @18:05:32.50 delay 5 seconds @18:05:32.57 Stroke completed -- waiting for pressure to equalize ... @18:05:32.58 SS.reconfigure :exhale @18:05:32.63 SS.hold @18:05:37.52 PSV.dial PTV @18:05:38.28 delay 5 seconds @18:05:43.31 PSV.dial :air @18:05:44.57 PS.seek 0ml @18:05:57.31 Intake.select :close @18:05:57.47 SSV.dial :exhaust @18:05:58.23 Exhaust.select :open @18:05:58.35 SS.seek 0ml @18:06:07.76 Exhaust.select :close @18:06:07.83 washCycleDA #2 of 3 @18:06:07.84 PSR.select :air @18:06:07.88 PSV.dial PSR @18:06:07.92 SSV.dial CBV @18:06:07.95 CBV.dial SSV @18:06:08.74 Allowing 130s for 25.0ml of remaining 350.0ml @18:06:08.76 Intake.select :open @18:06:09.34 Pulling 0.5ml of PSR.wash @18:06:09.35 PSR.select :wash @18:06:09.47 PS.reconfigure :default @18:06:09.52 PS.seek 0.5ml @18:06:10.93 Intake @16.6psia, vacuum from 10.0 to 13.0psi @18:06:10.95 SS.reconfigure :inhaling 0.30ml/s @18:06:11.00 SS.seek 25ml @18:06:15.42 delay 5 seconds @18:06:20.44 PSR.select :air @18:06:20.56 PS.seek 2ml @18:06:36.67 PTV.dial :puck @18:06:36.68 PSV.dial PTV @18:06:37.43 PBV.dial :waste1 @18:06:37.87 PS.seek 1.5ml @18:06:43.86 delay 3 seconds @18:06:46.88 PBV.dial between PSV and waste1 @18:06:47.25 PS.seek 0ml @18:07:03.36 delay 2:00 @18:07:29.95 Vacuum too high -- waiting up to 40 seconds @18:07:29.97 SS.reconfigure :exhale @18:07:30.03 SS.hold @18:07:36.95 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:07:36.97 SS.reconfigure :inhaling 0.28ml/s @18:07:37.02 SS.seek 25ml @18:07:45.60 Stroke completed -- waiting for pressure to equalize ... @18:07:45.61 SS.reconfigure :exhale @18:07:45.66 SS.hold @18:08:06.07 Intake.select :close @18:08:06.24 SSV.dial :exhaust @18:08:07.03 Exhaust.select :open @18:08:07.14 SS.seek 0ml @18:08:16.54 Exhaust.select :close @18:08:16.65 SSV.dial CBV @18:08:16.67 CBV.dial SSV @18:08:17.46 Allowing 130s for 25.0ml of remaining 325.0ml @18:08:17.48 Intake.select :open @18:08:19.64 Intake @16.6psia, vacuum from 10.0 to 13.0psi @18:08:19.66 SS.reconfigure :inhaling 0.28ml/s @18:08:19.71 SS.seek 25ml @18:09:03.38 recoverPSRreagent08 @18:09:03.39 PTV.dial :puck @18:09:03.40 PBV.dial :waste1 @18:09:03.78 delay 30 seconds @18:09:33.80 PBV.dial between PSV and waste1 @18:09:34.18 PSR.select :air @18:09:34.20 PSV.dial PSR @18:09:35.08 PS.reconfigure :fast @18:09:35.13 PS.seek 2.5ml @18:09:56.94 Stroke completed -- waiting for pressure to equalize ... @18:09:56.95 SS.reconfigure :exhale @18:09:57.00 SS.hold @18:09:58.65 PSV.dial PTV @18:09:59.91 delay 5 seconds @18:10:04.94 PSV.dial PSR @18:10:05.70 delay 5 seconds @18:10:10.72 PSV.dial PTV @18:10:11.46 delay 5 seconds @18:10:16.48 PSV.dial :air @18:10:17.25 PS.seek 0ml @18:10:27.49 SS.reconfigure :default @18:10:27.54 SS.hold @18:10:27.60 Intake.select :close @18:10:27.77 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 700.0ml @18:10:27.78 SSV.dial :exhaust @18:10:28.54 Exhaust.select :open @18:10:28.65 SS.reconfigure :exhale @18:10:28.70 SS.seek 0ml @18:10:38.27 Exhaust.select :close @18:10:38.38 Sampled 700.0ml @18:10:38.40 Drying puck before fixing for 4:00 @18:10:38.41 CBV.dial :waste2b @18:10:39.17 delay 1:00 @18:10:40.60 washCycleDA #3 of 3 @18:10:40.62 PSR.select :air @18:10:40.63 PSV.dial PSR @18:10:41.96 Pulling 0.5ml of PSR.wash @18:10:41.97 PSR.select :wash @18:10:42.10 PS.reconfigure :default @18:10:42.15 PS.seek 0.5ml @18:10:48.13 delay 5 seconds @18:10:53.16 PSR.select :air @18:10:53.28 PS.seek 2ml @18:11:09.38 PTV.dial :puck @18:11:09.40 PSV.dial PTV @18:11:10.13 PBV.dial :waste1 @18:11:10.57 PS.seek 1.5ml @18:11:16.57 delay 3 seconds @18:11:19.59 PBV.dial between PSV and waste1 @18:11:19.97 PS.seek 0ml @18:11:36.10 delay 2:00 @18:11:39.19 CSV.dial :air @18:11:39.68 CS.reconfigure :fast @18:11:39.73 CS.seek 10ml @18:12:03.92 Pumping wcr puck from tube 3 up to 35psi... @18:12:03.94 CBV.dial SSV @18:12:05.21 CBV.dial between CSV and waste2b @18:12:06.19 CSV.dial between air and CTV @18:12:06.52 CS.seek 6.555ml @18:12:15.36 CTV.dial CSV,avoiding: :air @18:12:15.39 CSV.dial CTV @18:12:16.22 CS.reconfigure :pressurize @18:12:16.28 CS.seek 0ml @18:12:30.47 CBV.dial :waste2b @18:12:30.83 CS.reconfigure :default @18:12:30.88 CS.seek 0ml @18:12:34.83 delay 1:00 @18:13:34.86 CTV.dial :air,avoiding: :intake @18:13:36.12 recoverPSRreagent08 @18:13:36.14 PTV.dial :puck @18:13:36.15 PBV.dial :waste1 @18:13:36.22 Fix #1 of 1 (with RNAlater) for 4:00 @18:13:36.24 CSV.dial CSR @18:13:36.53 delay 30 seconds @18:13:36.67 Pulling 2ml of CSR.RNAlater @18:13:36.68 CSR.select :RNAlater @18:13:36.80 CS.seek 2ml @18:13:43.04 delay 5 seconds @18:13:48.53 CSR.select :air @18:13:48.67 CS.reconfigure :fast @18:13:48.72 CS.seek 10ml @18:14:06.55 PBV.dial between PSV and waste1 @18:14:06.93 PSR.select :air @18:14:06.95 PSV.dial PSR @18:14:07.70 PS.reconfigure :fast @18:14:07.75 PS.seek 2.5ml @18:14:08.08 Pumping wcr puck from tube 3 up to 35psi... @18:14:08.10 CBV.dial SSV @18:14:09.36 CBV.dial between CSV and waste2b @18:14:10.35 CSV.dial between air and CTV @18:14:10.96 CS.seek 6.471ml @18:14:19.99 CTV.dial CSV,avoiding: :air @18:14:20.01 CSV.dial CTV @18:14:21.43 CS.reconfigure :pressurize @18:14:21.48 CS.seek 0ml @18:14:31.38 PSV.dial PTV @18:14:32.13 delay 5 seconds @18:14:35.19 delay 4:00 @18:14:37.15 PSV.dial PSR @18:14:37.90 delay 5 seconds @18:14:42.92 PSV.dial PTV @18:14:43.66 delay 5 seconds @18:14:48.69 PSV.dial :air @18:14:49.47 PS.seek 0ml @18:15:12.57 Gate.power :camera,:ON @18:15:12.58 Mixing DA Substrates @18:15:12.64 PSR.select :air @18:15:12.66 PSV.dial PSR @18:15:14.04 Pulling 0.25ml of PSR.sub1 @18:15:14.06 PSR.select :sub1 @18:15:14.30 PS.reconfigure :default @18:15:14.42 PS.seek 0.25ml @18:15:17.80 delay 5 seconds @18:15:22.82 Pulling 0.25ml of PSR.sub2 @18:15:22.83 PSR.select :sub2 @18:15:22.95 PS.seek 0.5ml @18:15:26.38 delay 5 seconds @18:15:31.40 PSR.select :air @18:15:31.52 PS.seek 2ml @18:15:47.66 PTV.dial :puck @18:15:47.67 PSV.dial PTV @18:15:48.43 PBV.dial :waste1 @18:15:48.88 PS.seek 1.5ml @18:15:54.76 delay 3 seconds @18:15:57.78 PBV.dial between PSV and waste1 @18:15:58.17 PS.seek 0ml @18:16:14.38 delay 10 seconds @18:16:24.41 recoverPSRreagent08 @18:16:24.42 PTV.dial :puck @18:16:24.43 PBV.dial :waste1 @18:16:24.82 delay 30 seconds @18:16:54.85 PBV.dial between PSV and waste1 @18:16:55.24 PSR.select :air @18:16:55.26 PSV.dial PSR @18:16:56.51 PS.reconfigure :fast @18:16:56.56 PS.seek 2.5ml @18:17:20.07 PSV.dial PTV @18:17:20.87 delay 5 seconds @18:17:25.89 PSV.dial PSR @18:17:26.76 delay 5 seconds @18:17:31.78 PSV.dial PTV @18:17:32.52 delay 5 seconds @18:17:37.54 PSV.dial :air @18:17:38.31 PS.seek 0ml @18:18:01.50 Additional air clear @18:18:01.51 PSR.select :air @18:18:01.53 PSV.dial PSR @18:18:02.89 Pulling 2ml of PSR.air @18:18:02.91 PSR.select :air @18:18:02.93 PS.reconfigure :default @18:18:02.98 PS.seek 2ml @18:18:24.22 delay 5 seconds @18:18:29.24 PTV.dial :puck @18:18:29.26 PSV.dial PTV @18:18:30.02 PBV.dial :waste1 @18:18:30.47 PS.seek 1.5ml @18:18:35.21 CBV.dial :waste2b @18:18:35.56 CS.reconfigure :default @18:18:35.61 CS.seek 0ml @18:18:36.41 delay 3 seconds @18:18:39.43 PBV.dial between PSV and waste1 @18:18:39.73 delay 1:00 @18:18:39.82 delay 1 second @18:18:40.84 PSV.dial :waste1 @18:18:41.89 PS.seek 0ml @18:18:58.03 Processing Puck Heater at 27.9C before imaging @18:18:58.04 PH.coast @18:18:58.15 Imaging da puck in Processing Clamp while storing it to tube 2 @18:18:58.33 SC.reconfigure :tight @18:18:58.39 SC.seek 2 @18:19:02.21 SC.configure :limp @18:19:02.43 Elbow.reconfigure :tight @18:19:02.48 Elbow.seek 2 @18:19:04.17 Forearm.reconfigure :tight @18:19:04.22 Forearm.seek 2 + 800 counts @18:19:05.35 SE.reconfigure :pick @18:19:05.41 SE.seek :beamBreak @18:19:07.04 SE.configure :default @18:19:07.20 Elbow.reconfigure :limp @18:19:07.25 Forearm.reconfigure :loose @18:19:07.30 Forearm.seek 2 @18:19:07.88 Elbow.hold @18:19:07.95 Elbow.reconfigure :tight @18:19:08.05 SE.seek 15.09 Pucks below Top Plate @18:19:08.54 Forearm.force -20 @18:19:08.59 Forearm.seek 2 + 297 counts @18:19:08.97 Forearm.reconfigure :tight @18:19:09.02 Forearm.seek 2 + 800 counts @18:19:09.26 SE.seek 16.09 Pucks below Top Plate @18:19:09.81 Forearm.reconfigure :loose @18:19:09.86 Forearm.seek :retract @18:19:10.79 Elbow.seek PC @18:19:13.28 Elbow.reconfigure :loose @18:19:13.38 Forearm.seek PC @18:19:14.50 Hand.close @18:19:15.21 PC.seek :open @18:19:19.78 Forearm.seek :retract @18:19:20.85 Elbow.reconfigure :tight @18:19:20.90 Elbow.seek 2 @18:19:23.56 Forearm.reconfigure :tight @18:19:23.61 Forearm.seek 2 + 800 counts @18:19:24.75 Hand.open @18:19:25.52 SE.seek 15.85 Pucks below Top Plate @18:19:25.79 SE.reconfigure :seal @18:19:25.84 SE.seek 15.19 Pucks below Top Plate @18:19:26.36 Lights, camera, ACTION! @18:19:26.40 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da25oct2918h625ml.tif @18:19:39.75 CTV.dial :air,avoiding: :intake @18:19:45.89 Waiting >20 seconds for da to relinquish FlushPuck @18:19:49.09 SX HX9/16 (USB:09): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 19.377 seconds /var/log/esp/da25oct2918h625ml.tif: TIFF Upload Complete @18:19:49.11 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da25oct2918h625ml15s.tif @18:20:04.72 SX HX9/16 (USB:09): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da25oct2918h625ml15s.tif: TIFF Upload Complete @18:20:04.75 PuckCamera: snap -bin=2,2 21.0 /var/log/esp/midres/da25oct2918h625ml21s.tif @18:20:26.37 SX HX9/16 (USB:09): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21 seconds /var/log/esp/midres/da25oct2918h625ml21s.tif: TIFF Upload Complete @18:20:26.38 Gate.power :camera,:OFF @18:20:26.42 DAfans.email "19.377 sec auto-exposure",Subject: "da25oct2918h625ml images!" @18:20:26.83 Elbow.seek 2 @18:20:27.71 Forearm.seek 2 + 800 counts @18:20:27.83 SE.reconfigure :gently @18:20:27.89 SE.seek 15.91 Pucks below Top Plate @18:20:28.66 Elbow.reconfigure :limp @18:20:28.72 Forearm.reconfigure :loose @18:20:28.77 Forearm.seek 2 @18:20:29.19 Elbow.hold @18:20:29.30 Elbow.reconfigure :tight @18:20:29.36 SE.reconfigure :default @18:20:29.41 SE.seek 16.09 Pucks below Top Plate @18:20:29.77 Forearm.force -20 @18:20:29.82 Forearm.seek 2 + 308 counts @18:20:30.44 Forearm.reconfigure :tight @18:20:30.56 Forearm.seek 2 + 800 counts @18:20:30.91 SE.seek :down @18:20:32.18 SE.stop @18:20:32.26 Forearm.reconfigure :loose @18:20:32.43 Forearm.seek :retract @18:20:33.62 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:20:34.33 Elbow.seek FlushPuck::Garage @18:20:36.24 Elbow.reconfigure :loose @18:20:36.45 Forearm.seek :garage @18:20:37.55 Hand.close @18:20:38.70 Forearm.seek :retract @18:20:39.74 Elbow.reconfigure :tight @18:20:39.83 Elbow.seek PC @18:20:41.24 Elbow.reconfigure :loose @18:20:42.42 Forearm.reconfigure :tight @18:20:42.59 Forearm.seek PC @18:20:44.46 PC.seek :closed @18:20:49.04 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.58 @18:20:49.17 Hand.open @18:20:50.11 Forearm.reconfigure :loose @18:20:50.20 Forearm.seek :retract @18:20:51.40 PSR.select :air @18:20:51.46 Storing wcr puck in Collection Clamp to tube 2 @18:20:51.50 PSV.dial PSR @18:20:51.96 SC.reconfigure :tight @18:20:52.08 SC.seek 2 @18:20:52.23 Pulling 0.5ml of PSR.flush @18:20:52.26 PSR.select :flush @18:20:52.38 SC.configure :limp @18:20:52.77 PS.seek 0.5ml @18:20:53.04 Elbow.reconfigure :tight @18:20:53.16 Elbow.seek 2 @18:20:55.93 Forearm.reconfigure :tight @18:20:56.04 Forearm.seek 2 + 800 counts @18:20:57.25 SE.reconfigure :pick @18:20:57.38 SE.seek :beamBreak @18:20:58.77 delay 5 seconds @18:20:59.12 SE.configure :default @18:20:59.47 Elbow.reconfigure :limp @18:20:59.58 Forearm.reconfigure :loose @18:21:00.63 Forearm.seek 2 @18:21:01.16 Elbow.hold @18:21:01.29 Elbow.reconfigure :tight @18:21:01.52 SE.seek 16.09 Pucks below Top Plate @18:21:02.17 Forearm.force -20 @18:21:02.27 Forearm.seek 2 + 293 counts @18:21:02.86 Forearm.reconfigure :tight @18:21:02.97 Forearm.seek 2 + 800 counts @18:21:03.34 SE.seek 17.09 Pucks below Top Plate @18:21:03.80 PSR.select :air @18:21:03.86 upsync @18:21:03.98 PS.seek 2.5ml @18:21:04.12 Forearm.reconfigure :loose @18:21:04.17 Forearm.seek :retract @18:21:05.15 Elbow.seek CC @18:21:06.76 Elbow.reconfigure :loose @18:21:06.88 Forearm.seek CC @18:21:07.85 Hand.close @18:21:08.58 CC.seek :open @18:21:12.81 Forearm.seek :retract @18:21:13.83 Elbow.reconfigure :tight @18:21:13.89 Elbow.seek 2 @18:21:15.60 Elbow.reconfigure :limp @18:21:15.66 Forearm.seek 2 @18:21:16.80 Elbow.hold @18:21:16.87 Elbow.reconfigure :tight @18:21:16.92 Hand.open @18:21:18.29 Forearm.force -20 @18:21:18.34 Forearm.seek 2 + 314 counts @18:21:18.71 Forearm.reconfigure :tight @18:21:18.76 Forearm.seek 2 + 800 counts @18:21:19.12 SE.seek :down @18:21:20.27 SE.stop @18:21:20.30 Forearm.reconfigure :loose @18:21:20.39 Forearm.seek :retract @18:21:21.29 Starting finalPurge @18:21:25.28 delay 1 second @18:21:26.30 PRV.dial :waste2,avoiding: :ab2 @18:21:28.16 PTV.dial :PRVmixing @18:21:29.44 PSV.dial :mixloop @18:21:30.48 PS.seek :empty @18:21:40.45
Waiting >20 seconds for psFlush to relinquish FlushPuck @18:21:56.91 PSR.select :air @18:21:56.93 PSV.dial PSR @18:21:57.97 Pulling 0.5ml of PSR.flush @18:21:57.98 PSR.select :flush @18:21:58.10 PS.seek 0.5ml @18:22:04.06 delay 5 seconds @18:22:09.08 PSR.select :air @18:22:09.21 PS.seek 2.5ml @18:22:30.50 delay 1 second @18:22:31.52 PBV.dial :waste1 @18:22:31.91 PTV.dial :mixing @18:22:32.66 PSV.dial :mixloop @18:22:33.71 PS.seek :empty @18:23:00.28 PSV.dial PSR @18:23:01.33 PSR.select :flush @18:23:01.45 PS.seek 1ml @18:23:12.53 PSR.select :air @18:23:12.65 PS.seek 2.5ml @18:23:28.75 PSV.dial PTV @18:23:29.50 PTV.dial :puck @18:23:30.23 PBV.dial :waste1 @18:23:30.32 PS.seek 0ml @18:23:56.72 PSV.dial PSR @18:23:57.47 PSR.select :flush @18:23:57.59 PS.seek 0.5ml @18:24:03.59 PSR.select :air @18:24:04.16 PS.seek 2.5ml @18:24:25.40 PSV.dial PTV @18:24:26.16 PBV.dial PSV @18:24:26.74 PS.seek 0ml @18:24:53.15 PSV.dial PBV @18:24:53.63 PS.seek 2.5ml @18:25:20.03 PSV.dial :waste1 @18:25:20.77 PS.seek 0ml @18:25:47.09 PSV.dial PSR @18:25:47.56 PSR.select :air @18:25:47.59 PS.seek 2.5ml @18:26:14.25 PSV.dial PTV @18:26:14.98 PBV.dial PSV @18:26:15.18 PS.seek 0ml @18:26:41.53 PSV.dial PBV @18:26:42.00 PS.seek 2.5ml @18:27:08.37 PSV.dial :waste1 @18:27:09.11 PS.seek 0ml @18:27:35.46 PSV.dial PSR @18:27:35.93 PSR.select :flush @18:27:36.05 PS.seek 1ml @18:27:47.18 PSR.select :air @18:27:47.30 PS.seek 2.5ml @18:28:03.50 PSV.dial PTV @18:28:04.23 PTV.dial :puck @18:28:04.31 PBV.dial :waste1 @18:28:04.86 PS.seek 0ml @18:28:31.25 PSV.dial PSR @18:28:32.00 PSR.select :flush @18:28:32.12 PS.seek 0.5ml @18:28:38.12 PSR.select :air @18:28:38.24 PS.seek 2.5ml @18:28:59.56 PSV.dial PTV @18:29:00.31 PBV.dial PSV @18:29:00.88 PS.seek 0ml @18:29:27.46 PSV.dial PBV @18:29:28.05 PS.seek 2.5ml @18:29:54.37 PSV.dial :waste1 @18:29:55.13 PS.seek 0ml @18:30:21.43 PSV.dial PSR @18:30:21.88 PSR.select :air @18:30:21.91 PS.seek 2.5ml @18:30:48.21 PSV.dial PTV @18:30:49.33 PBV.dial PSV @18:30:49.49 PS.seek 0ml @18:31:16.06 PSV.dial PBV @18:31:16.64 PS.seek 2.5ml @18:31:42.99 PSV.dial :waste1 @18:31:43.75 PS.seek 0ml @18:32:10.06
Loading FlushPuck into Collection Clamp from Processing Clamp @18:32:10.25 Elbow.seek PC @18:32:12.78 Elbow.reconfigure :loose @18:32:12.89 Forearm.seek PC @18:32:13.97 Hand.close @18:32:14.70 PC.seek :open @18:32:19.21 Forearm.seek :retract @18:32:20.21 Elbow.reconfigure :tight @18:32:20.26 Elbow.seek CC @18:32:23.80 Elbow.reconfigure :loose @18:32:23.90 Forearm.reconfigure :tight @18:32:23.95 Forearm.seek CC @18:32:25.27 CC.seek :closed @18:32:29.28 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.04 @18:32:29.35 Hand.open @18:32:30.14 Forearm.reconfigure :loose @18:32:30.19 Forearm.seek :retract @18:32:31.18 Flushing the Collection Valve::Manifold @18:32:31.31 CSV.dial CSR @18:32:31.75 Pulling 5ml of CSR.flush @18:32:31.76 CSR.select :flush @18:32:31.88 CS.seek 5ml @18:32:45.68 delay 5 seconds @18:32:51.16 CSR.select :air @18:32:51.32 CS.reconfigure :fast @18:32:51.37 CS.seek 7.499ml @18:32:57.90 CSV.dial :waste @18:32:59.19 CS.reconfigure :default @18:32:59.24 CS.seek 0ml @18:33:19.18 Flushing lower manifold's waste1 port @18:33:19.31 CSV.dial :flush @18:33:19.75 CS.reconfigure :fast @18:33:19.80 CS.seek 5ml @18:33:32.18 delay 5 seconds @18:33:37.20 CSV.dial :air @18:33:37.72 CS.seek 7ml @18:33:43.40 CSV.dial CTV @18:33:43.43
CTV.dial CSV @18:33:44.84 CBV.dial :waste1 @18:33:45.61 CS.seek 0ml @18:34:02.40 Flushing lower manifold's waste2 port @18:34:02.52 CSV.dial :flush @18:34:03.27 CS.seek 5ml @18:34:15.78 delay 5 seconds @18:34:20.80 CSV.dial :air @18:34:21.32 CS.seek 7ml @18:34:26.75 CSV.dial CTV @18:34:26.77
CTV.dial CSV @18:34:27.23 CBV.dial :waste2 @18:34:28.28 CS.seek 0ml @18:34:45.06 Flushing lower manifold's waste2b port @18:34:45.18 CSV.dial :flush @18:34:45.92 CS.seek 5ml @18:34:58.43 delay 5 seconds @18:35:03.45 CSV.dial :air @18:35:03.98 CS.seek 7ml @18:35:09.40 CSV.dial CTV @18:35:09.43
CTV.dial CSV @18:35:09.88 CBV.dial :waste2b @18:35:10.92 CS.seek 0ml @18:35:27.68 Disinfecting external Intake with kill @18:35:27.80 CSV.dial CSR @18:35:28.24 Pulling 8ml of CSR.kill @18:35:28.25 CSR.select :kill @18:35:28.37 CS.seek 8ml @18:35:47.56 delay 5 seconds @18:35:52.58 CSR.select :air @18:35:52.70 CS.seek 10ml @18:35:58.68 CSV.dial CBV @18:35:58.71
CBV.dial CSV @18:35:59.76 CTV.dial :intake @18:36:00.52 Intake.select :open @18:36:00.64 CS.seek 2ml @18:36:19.59 Intake.select :close @18:36:19.69 CTV.dial :air @18:36:20.47 CS.reconfigure :default @18:36:20.52 CS.seek 0ml @18:36:26.49 CSV.dial CSR @18:36:27.51 Pulling 5ml of CSR.flush @18:36:27.52 CSR.select :flush @18:36:27.64 CS.seek 5ml @18:36:41.24 delay 5 seconds @18:36:46.26 CSR.select :air @18:36:46.38 CS.seek 10ml @18:37:00.02 CSV.dial CBV @18:37:01.16 CS.seek 0ml @18:37:27.59 Garaging FlushPuck in Collection Clamp @18:37:27.66 Elbow.reconfigure :tight @18:37:27.71 Elbow.seek CC @18:37:27.83 Elbow.reconfigure :loose @18:37:27.94 Forearm.seek CC @18:37:28.89 Hand.close @18:37:29.69 CC.seek :open @18:37:33.80 Forearm.seek :retract @18:37:34.78 Elbow.reconfigure :tight @18:37:34.83 Elbow.seek :garage @18:37:37.66 Elbow.reconfigure :loose @18:37:37.76 Forearm.reconfigure :tight @18:37:37.81 Forearm.seek :garage @18:37:38.92 Hand.open @18:37:39.75 Forearm.reconfigure :loose @18:37:39.80 Forearm.seek :retract @18:37:40.80 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 16:23:51 @18:37:40.85 Asleep after completing DA_WCR_102925 assays @18:37:40.87 GoodNews.email "{1 => 22,\n 2 => 18,\n 3 => 4,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@18:33:18, 40% flow, 20.7C, 23% humidity, 14.6psia, 13.71V, 0.384A avg, 218.855Ah, 5.27W\nSampled 700.0ml",Subject: "Asleep after completing DA_WCR_102925 assays" @18:37:44.83 upsync + ESPwhich DA_WCR_110525.rb + fn=DA_WCR_110525.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_110525.rb + [ -f /home/esp/esp2/mission/DA_WCR_110525.rb ] + realpath /home/esp/esp2/mission/DA_WCR_110525.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_110525.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_110525.rb @18:38:03.00UTC05-Nov-25
ESP2 v3.42 I2C Gateway v3.20 for core bus @18:38:05.65 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @18:38:09.27 Polling Can every 10:00 @18:38:09.35 Cleanly exited at 13:43:04UTC05-Nov-25 @18:38:09.39 Loading /home/esp/esp2local/mission/DA_WCR_110525.rb mission @18:38:09.45 Preparing to sleep until + 00:00:05 @18:38:09.47 GoodNews.email "Starting DA_WCR_110525 w/pucks in tube 3\n{1 => 22,\n 2 => 18,\n 3 => 4,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@18:38:09, 41% flow, 19.1C, 25% humidity, 14.5psia, 13.74V, 0.252A avg, 254.895Ah, 3.47W",Subject: "DA_WCR_110525 begun" @18:38:09.53
Asleep til + 00:00:05 @18:38:09.55 Continuing at 18:38 @18:38:09.80 Gate.power :core,:OFF @18:38:09.81 Gate.power :raw,:OFF @18:38:09.82 Shutdown @18:38:12.14 GoodNews.email "Then, in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_110525.rb:14\n{1 => 22,\n 2 => 18,\n 3 => 4,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@18:38:09, 41% flow, 19.1C, 25% humidity, 14.5psia, 13.74V, 0.252A avg, 254.895Ah, 3.47W",Subject: "Asleep until 18:38" @18:38:13.55 upsync @18:38:14.44
Powering core bus @18:38:16.00 upsync @18:38:19.78
Gate.power :raw,:ON @18:38:20.64 CS.configure :default @18:38:20.69 PS.configure :default @18:38:20.74 SS.configure :default @18:38:20.79 CC.configure :default @18:38:20.83 PC.configure :default @18:38:20.90 Forearm.configure :loose @18:38:20.95 Elbow.configure :loose @18:38:21.02 SC.configure :loose @18:38:21.07 SE.configure :default @18:38:21.12 CH.configure :default @18:38:21.15 PH.configure :default @18:38:21.19 All dwarf controllers running firmware version 3.85 @18:38:21.30 Valve.reset @18:38:21.59 Intake.select :close @18:38:21.63 Exhaust.select :close @18:38:21.64 CTV.dial :air @18:38:21.75 PSV.dial :air @18:38:21.86 CSV.dial :waste1 @18:38:21.89
Forearm.home.seek :retract @18:38:22.36 SSV.dial :spit,avoiding: :exhaust @18:38:22.63 Hand.close @18:38:22.67
Elbow.reconfigure :loose @18:38:22.76 Elbow.jog 1500 @18:38:22.83 PS.home.seek 0ml @18:38:23.16
Elbow.reconfigure :loose @18:38:23.21 Elbow.home.seek :clear @18:38:23.29 CS.reconfigure :fast @18:38:23.33 SS.home.seek 0ml @18:38:23.41 CS.home.seek 0ml @18:38:26.45
SE.home.seek :down @18:38:27.12 SC.reconfigure :tight @18:38:27.17 SC.home.seek :initial @18:38:31.89 PS.stop @18:38:39.90
SC.configure :limp @18:38:39.95 Forearm.seek :retract @18:38:40.02 Elbow.seek :hammer @18:38:41.04 Forearm.reconfigure :tight @18:38:41.09 Forearm.seek retracted @18:38:41.81 CC.home.seek :open @18:38:41.89 PC.home.seek :open @18:38:45.05
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @18:38:45.07 Notice.email "At 18:38:44 began in tube 3: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_110525.rb:14\n{1 => 22,\n 2 => 18,\n 3 => 4,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@18:38:45, 41% flow, 19.0C, 25% humidity, 14.6psia, 13.74V, 0.259A avg, 254.899Ah, 3.55W",Subject: "Started DA_WCR_110525 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @18:38:45.10
Starting initialPurge @18:38:45.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:38:45.18 Hand.open @18:38:46.02 Elbow.reconfigure :tight @18:38:46.07 Elbow.seek FlushPuck::Garage @18:38:47.92 Elbow.reconfigure :loose @18:38:48.04 Forearm.reconfigure :loose @18:38:48.09 Forearm.seek :garage @18:38:48.91 upsync @18:38:49.07
Hand.close @18:38:49.88 Forearm.seek :retract @18:38:50.93 Elbow.reconfigure :tight @18:38:50.98 Elbow.seek CC @18:38:53.88 Elbow.reconfigure :loose @18:38:53.98 Forearm.reconfigure :tight @18:38:54.03 Forearm.seek CC @18:38:55.26 CC.seek :closed @18:38:59.23 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.93 @18:38:59.30 Hand.open @18:39:00.06 Forearm.reconfigure :loose @18:39:00.11 Forearm.seek :retract @18:39:01.08 Flushing the Collection Valve::Manifold @18:39:01.21 CSV.dial CSR @18:39:02.49 Pulling 5ml of CSR.flush CSR.select :flush @18:39:02.61 CS.reconfigure :default @18:39:02.66 CS.seek 5ml @18:39:16.91 delay 5 seconds @18:39:21.93 CSR.select :air @18:39:22.10 CS.reconfigure :fast @18:39:22.15 CS.seek 7.5ml @18:39:28.81 CSV.dial :waste @18:39:30.10 CS.reconfigure :default @18:39:30.15 CS.seek 0ml @18:39:50.19 Purging external Intake @18:39:50.31 CSV.dial :flush @18:39:50.76 CS.reconfigure :fast @18:39:50.80 CS.seek 10ml @18:40:14.75 delay 5 seconds @18:40:19.77 CSV.dial CBV @18:40:19.81
CBV.dial CSV @18:40:20.56 CTV.dial :intake @18:40:21.34 Intake.select :open @18:40:21.45 CS.seek 0ml @18:40:45.25 Intake.select :close @18:40:45.35 CTV.dial :air @18:40:46.16 Flushing upper manifold @18:40:46.28 CSV.dial :flush @18:40:47.02 CS.seek 5ml @18:40:59.37 delay 5 seconds @18:41:04.39 CSV.dial :air @18:41:04.89 CS.seek 7ml @18:41:10.47 CSV.dial CBV @18:41:10.50
CBV.dial CSV @18:41:11.53 CTV.dial :air @18:41:11.62 CS.seek 0ml @18:41:28.44 delay 7 seconds @18:41:35.47 CS.seek 3ml @18:41:43.28 CSV.dial :waste @18:41:43.74 CS.seek 0ml @18:41:51.55 CSV.dial :flush @18:41:51.99 CS.seek 3ml @18:41:59.81 delay 5 seconds @18:42:04.84 CSV.dial :air @18:42:05.35 CS.seek 5ml @18:42:10.83 CSV.dial :air @18:42:10.91 CS.seek 0ml @18:42:23.25 delay 7 seconds @18:42:30.28 CS.seek 3ml @18:42:38.06 CSV.dial :waste @18:42:38.80 CS.seek 0ml @18:42:46.63 Garaging FlushPuck in Collection Clamp @18:42:46.70 Elbow.reconfigure :tight @18:42:46.75 Elbow.seek CC @18:42:46.94 Elbow.reconfigure :loose @18:42:47.05 Forearm.seek CC @18:42:48.00 Hand.close @18:42:48.75 CC.seek :open @18:42:52.90 Forearm.seek :retract @18:42:53.89 Elbow.reconfigure :tight @18:42:53.94 Elbow.seek :garage @18:42:56.80 Elbow.reconfigure :loose @18:42:56.91 Forearm.reconfigure :tight @18:42:56.96 Forearm.seek :garage @18:42:58.00 Hand.open @18:42:58.81 Forearm.reconfigure :loose @18:42:58.86 Forearm.seek :retract @18:42:59.86 da sampling at most 1000ml, wcr at most 1000ml @18:42:59.90 Loading sh1 puck from tube 3 into Collection Clamp @18:42:59.92 DAfans.email "Started da in tube 3 at 18:42:59UTC05-Nov-25\n{1 => 22,\n 2 => 18,\n 3 => 4,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@18:38:45, 41% flow, 19.0C, 25% humidity, 14.6psia, 13.74V, 0.259A avg, 254.899Ah, 3.55W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @18:43:00.20
SC.reconfigure :tight @18:43:00.25 SC.seek 3 @18:43:03.93 SC.configure :limp @18:43:04.18 upsync @18:43:04.21
Elbow.reconfigure :tight @18:43:04.26 Elbow.seek 3 @18:43:06.28 Forearm.reconfigure :tight @18:43:06.33 Forearm.seek 3 + 800 counts @18:43:07.41 SE.reconfigure :pick @18:43:07.47 SE.seek :beamBreak @18:43:10.15 SE.configure :default @18:43:10.32 Elbow.seek 3 @18:43:10.53 Forearm.seek 3 + 300 counts @18:43:10.70 Forearm.reconfigure :loose @18:43:10.75 Hand.close @18:43:11.52 SE.seek :down @18:43:12.16
Forearm.seek :retract @18:43:13.13 Elbow.seek CC @18:43:14.03 SE.stop @18:43:14.72
Elbow.reconfigure :loose @18:43:14.82 Forearm.reconfigure :tight @18:43:14.87 Forearm.seek CC @18:43:16.26 CC.seek :closed @18:43:20.26 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.05 @18:43:20.27 Hand.open @18:43:21.07 Forearm.reconfigure :loose @18:43:21.11 Forearm.seek :retract @18:43:22.20 collectSample 1000ml w/bubble point of 24psi @18:43:22.21 Priming 7ml @18:43:22.22 Intake.select :close @18:43:22.24 Exhaust.select :close @18:43:22.25 SS.hold @18:43:22.45 Calibrating pressure and temperature sensors @18:43:22.48 CTV.dial :air @18:43:22.56 SSV.dial :spit,avoiding: :exhaust @18:43:22.65 SS.reconfigure :fast @18:43:22.70 SS.seek 5ml @18:43:25.73 SSV.dial CBV @18:43:25.75
CBV.dial SSV @18:43:27.13 delay 3.8 seconds @18:43:31.06 Sensor limits: Intake max 95.7psia; Outlet max 109.9psia @18:43:31.07 SSV.dial :spit @18:43:32.43 SS.seek 0ml @18:43:35.24 SSV.dial CBV @18:43:36.58 CTV.dial :intake @18:43:37.36 SSV.dial CBV @18:43:37.39
CBV.dial SSV @18:43:37.60 Allowing 40s for 7.0ml of remaining 7.0ml @18:43:37.62 Intake.select :open @18:43:39.78 Intake @14.5psia, vacuum from 9.0 to 12.0psi @18:43:39.80 SS.reconfigure :inhaling 2.17ml/s @18:43:39.85 SS.seek 7ml @18:43:45.50 Stroke completed -- waiting for pressure to equalize ... @18:43:45.51 SS.reconfigure :exhale @18:43:45.56 SS.hold @18:43:47.60 Intake.select :close @18:43:47.77 SSV.dial :exhaust @18:43:48.54 Exhaust.select :open @18:43:48.65 SS.seek 0ml @18:43:52.52 Exhaust.select :close @18:43:52.63 Sampling 1000.0ml @18:43:52.64 Intake.select :close @18:43:52.65 Exhaust.select :close @18:43:52.66 SS.reconfigure :default @18:43:52.71 SS.hold @18:43:52.92 CTV.dial :intake @18:43:53.09 SSV.dial CBV @18:43:53.12
CBV.dial SSV @18:43:53.93 Allowing 130s for 25.0ml of remaining 1000.0ml @18:43:53.94 Intake.select :open @18:43:56.11 Intake @14.2psia, vacuum from 8.7 to 11.7psi @18:43:56.12 SS.reconfigure :inhaling 2.17ml/s @18:43:56.17 SS.seek 25ml @18:44:11.24 Stroke completed -- waiting for pressure to equalize ... @18:44:11.25 SS.reconfigure :exhale @18:44:11.29 SS.hold @18:44:19.38 Intake.select :close @18:44:19.54 SSV.dial :exhaust @18:44:20.30 Exhaust.select :open @18:44:20.42 SS.seek 0ml @18:44:29.71 Exhaust.select :close @18:44:29.82 SSV.dial CBV @18:44:29.84
CBV.dial SSV @18:44:30.65 Allowing 130s for 25.0ml of remaining 975.0ml @18:44:30.66 Intake.select :open @18:44:32.82 Intake @13.9psia, vacuum from 8.4 to 11.4psi @18:44:32.84 SS.reconfigure :inhaling 2.17ml/s @18:44:32.89 SS.seek 25ml @18:44:46.83 Stroke completed -- waiting for pressure to equalize ... @18:44:46.84 SS.reconfigure :exhale @18:44:47.31 SS.hold @18:44:55.88 Intake.select :close @18:44:56.04 SSV.dial :exhaust @18:44:56.80 Exhaust.select :open @18:44:56.91 SS.seek 0ml @18:45:06.08 Exhaust.select :close @18:45:06.19 SSV.dial CBV @18:45:06.22
CBV.dial SSV @18:45:07.00 Allowing 130s for 25.0ml of remaining 950.0ml @18:45:07.01 Intake.select :open @18:45:09.18 Intake @13.6psia, vacuum from 8.1 to 11.1psi @18:45:09.19 SS.reconfigure :inhaling 2.17ml/s @18:45:09.24 SS.seek 25ml @18:45:23.97 Stroke completed -- waiting for pressure to equalize ... @18:45:23.98 SS.reconfigure :exhale @18:45:24.03 SS.hold @18:45:32.83 Intake.select :close @18:45:32.98 SSV.dial :exhaust @18:45:33.77 Exhaust.select :open @18:45:33.89 SS.seek 0ml @18:45:43.21 Exhaust.select :close @18:45:43.32 SSV.dial CBV @18:45:43.34
CBV.dial SSV @18:45:44.14 Allowing 130s for 25.0ml of remaining 925.0ml @18:45:44.16 Intake.select :open @18:45:46.32 Intake @14.0psia, vacuum from 8.5 to 11.5psi @18:45:46.34 SS.reconfigure :inhaling 2.17ml/s @18:45:46.39 SS.seek 25ml @18:46:00.28 Stroke completed -- waiting for pressure to equalize ... @18:46:00.29 SS.reconfigure :exhale @18:46:00.34 SS.hold @18:46:10.11 Intake.select :close @18:46:10.25 SSV.dial :exhaust @18:46:11.04 Exhaust.select :open @18:46:11.15 SS.seek 0ml @18:46:20.41 Exhaust.select :close @18:46:20.52 SSV.dial CBV @18:46:20.54
CBV.dial SSV @18:46:21.35 Allowing 130s for 25.0ml of remaining 900.0ml @18:46:21.36 Intake.select :open @18:46:23.53 Intake @13.9psia, vacuum from 8.4 to 11.4psi @18:46:23.54 SS.reconfigure :inhaling 2.17ml/s @18:46:23.59 SS.seek 25ml @18:46:36.15 Vacuum too high -- waiting up to 40 seconds @18:46:36.17 SS.reconfigure :exhale @18:46:36.22 SS.hold @18:46:36.93 Intake @10.0psia, vacuum from 4.5 to 7.5psi @18:46:36.94 SS.reconfigure :inhaling 1.95ml/s @18:46:36.99 SS.seek 25ml @18:46:39.16 Stroke completed -- waiting for pressure to equalize ... @18:46:39.17 SS.reconfigure :exhale @18:46:39.21 SS.hold @18:46:47.79 Intake.select :close @18:46:47.94 SSV.dial :exhaust @18:46:48.72 Exhaust.select :open @18:46:48.83 SS.seek 0ml @18:46:58.05 Exhaust.select :close @18:46:58.16 SSV.dial CBV @18:46:58.18
CBV.dial SSV @18:46:58.99 Allowing 130s for 25.0ml of remaining 875.0ml @18:46:59.00 Intake.select :open @18:47:01.16 Intake @13.9psia, vacuum from 8.4 to 11.4psi @18:47:01.18 SS.reconfigure :inhaling 1.95ml/s @18:47:01.23 SS.seek 25ml @18:47:16.59 Stroke completed -- waiting for pressure to equalize ... @18:47:16.60 SS.reconfigure :exhale @18:47:16.65 SS.hold @18:47:26.87 Intake.select :close @18:47:27.03 SSV.dial :exhaust @18:47:27.80 Exhaust.select :open @18:47:27.91 SS.seek 0ml @18:47:36.77 Exhaust.select :close @18:47:36.87 SSV.dial CBV @18:47:36.90
CBV.dial SSV @18:47:37.70 Allowing 130s for 25.0ml of remaining 850.0ml @18:47:37.71 Intake.select :open @18:47:39.88 Intake @14.1psia, vacuum from 8.6 to 11.6psi @18:47:39.89 SS.reconfigure :inhaling 1.95ml/s @18:47:39.94 SS.seek 25ml @18:47:54.98 Stroke completed -- waiting for pressure to equalize ... @18:47:54.99 SS.reconfigure :exhale @18:47:55.04 SS.hold @18:48:06.25 Intake.select :close @18:48:06.41 SSV.dial :exhaust @18:48:07.19 Exhaust.select :open @18:48:07.30 SS.seek 0ml @18:48:16.56 Exhaust.select :close @18:48:16.67 SSV.dial CBV @18:48:16.71
CBV.dial SSV @18:48:17.48 Allowing 130s for 25.0ml of remaining 825.0ml @18:48:17.49 Intake.select :open @18:48:19.66 Intake @13.9psia, vacuum from 8.4 to 11.4psi @18:48:19.67 SS.reconfigure :inhaling 1.95ml/s @18:48:19.72 SS.seek 25ml @18:48:34.68 Stroke completed -- waiting for pressure to equalize ... @18:48:34.70 SS.reconfigure :exhale @18:48:34.74 SS.hold @18:48:47.33 Intake.select :close @18:48:47.49 SSV.dial :exhaust @18:48:48.26 Exhaust.select :open @18:48:48.38 SS.seek 0ml @18:48:57.67 Exhaust.select :close @18:48:57.78 SSV.dial CBV @18:48:58.25
CBV.dial SSV @18:48:59.02 Allowing 130s for 25.0ml of remaining 800.0ml @18:48:59.03 Intake.select :open @18:49:01.20 Intake @14.3psia, vacuum from 8.8 to 11.8psi @18:49:01.21 SS.reconfigure :inhaling 1.95ml/s @18:49:01.26 SS.seek 25ml @18:49:16.20 Stroke completed -- waiting for pressure to equalize ... @18:49:16.21 SS.reconfigure :exhale @18:49:16.26 SS.hold @18:49:28.97 Intake.select :close @18:49:29.12 SSV.dial :exhaust @18:49:29.90 Exhaust.select :open @18:49:30.02 SS.seek 0ml @18:49:39.25 Exhaust.select :close @18:49:39.36 SSV.dial CBV @18:49:39.38
CBV.dial SSV @18:49:40.19 Allowing 130s for 25.0ml of remaining 775.0ml @18:49:40.20 Intake.select :open @18:49:42.36 Intake @13.8psia, vacuum from 8.3 to 11.3psi @18:49:42.38 SS.reconfigure :inhaling 1.95ml/s @18:49:42.43 SS.seek 25ml @18:49:57.40 Stroke completed -- waiting for pressure to equalize ... @18:49:57.41 SS.reconfigure :exhale @18:49:57.46 SS.hold @18:50:10.56 Intake.select :close @18:50:10.72 SSV.dial :exhaust @18:50:11.50 Exhaust.select :open @18:50:11.61 SS.seek 0ml @18:50:20.89 Exhaust.select :close @18:50:21.00 SSV.dial CBV @18:50:21.02
CBV.dial SSV @18:50:21.84 Allowing 130s for 25.0ml of remaining 750.0ml @18:50:21.85 Intake.select :open @18:50:24.02 Intake @14.2psia, vacuum from 8.7 to 11.7psi @18:50:24.04 SS.reconfigure :inhaling 1.95ml/s @18:50:24.09 SS.seek 25ml @18:50:39.33 Vacuum too high -- waiting up to 40 seconds @18:50:39.34 SS.reconfigure :exhale @18:50:39.40 SS.hold @18:50:39.83 Intake @11.1psia, vacuum from 5.6 to 8.6psi @18:50:39.84 SS.reconfigure :inhaling 1.77ml/s @18:50:39.89 SS.seek 25ml @18:50:40.03 Stroke completed -- waiting for pressure to equalize ... @18:50:40.04 SS.reconfigure :exhale @18:50:40.08 SS.hold @18:50:52.76 Intake.select :close @18:50:52.92 SSV.dial :exhaust @18:50:53.68 Exhaust.select :open @18:50:53.80 SS.seek 0ml @18:51:03.11 Exhaust.select :close @18:51:03.22 SSV.dial CBV @18:51:03.24
CBV.dial SSV @18:51:04.04 Allowing 130s for 25.0ml of remaining 725.0ml @18:51:04.05 Intake.select :open @18:51:06.22 Intake @14.2psia, vacuum from 8.7 to 11.7psi @18:51:06.23 SS.reconfigure :inhaling 1.77ml/s @18:51:06.28 SS.seek 25ml @18:51:22.43 Stroke completed -- waiting for pressure to equalize ... @18:51:22.44 SS.reconfigure :exhale @18:51:22.49 SS.hold @18:51:36.88 Intake.select :close @18:51:37.04 SSV.dial :exhaust @18:51:37.82 Exhaust.select :open @18:51:37.93 SS.seek 0ml @18:51:47.23 Exhaust.select :close @18:51:47.34 SSV.dial CBV @18:51:47.37
CBV.dial SSV @18:51:48.15 Allowing 130s for 25.0ml of remaining 700.0ml @18:51:48.16 Intake.select :open @18:51:50.33 Intake @14.2psia, vacuum from 8.7 to 11.7psi @18:51:50.34 SS.reconfigure :inhaling 1.77ml/s @18:51:50.39 SS.seek 25ml @18:52:06.52 Stroke completed -- waiting for pressure to equalize ... @18:52:06.53 SS.reconfigure :exhale @18:52:06.58 SS.hold @18:52:22.90 Intake.select :close @18:52:23.05 SSV.dial :exhaust @18:52:24.26 Exhaust.select :open @18:52:24.37 SS.seek 0ml @18:52:33.18 Exhaust.select :close @18:52:33.29 SSV.dial CBV @18:52:33.32
CBV.dial SSV @18:52:34.12 Allowing 130s for 25.0ml of remaining 675.0ml @18:52:34.13 Intake.select :open @18:52:36.30 Intake @14.2psia, vacuum from 8.7 to 11.7psi @18:52:36.32 SS.reconfigure :inhaling 1.77ml/s @18:52:36.37 SS.seek 25ml @18:52:48.14 Vacuum too high -- waiting up to 40 seconds @18:52:48.16 SS.reconfigure :exhale @18:52:48.21 SS.hold @18:52:49.09 Intake @11.6psia, vacuum from 6.1 to 9.1psi @18:52:49.11 SS.reconfigure :inhaling 1.59ml/s @18:52:49.16 SS.seek 25ml @18:52:52.86 Vacuum too high -- waiting up to 40 seconds @18:52:52.87 SS.reconfigure :exhale @18:52:52.93 SS.hold @18:53:02.98 Intake @12.3psia, vacuum from 6.8 to 9.8psi @18:53:02.99 SS.reconfigure :inhaling 1.43ml/s @18:53:03.04 SS.seek 25ml @18:53:06.82 Stroke completed -- waiting for pressure to equalize ... @18:53:06.83 SS.reconfigure :exhale @18:53:06.88 SS.hold @18:53:12.20 Intake.select :close @18:53:12.36 SSV.dial :exhaust @18:53:13.13 Exhaust.select :open @18:53:13.25 SS.seek 0ml @18:53:22.37 Exhaust.select :close @18:53:22.48 SSV.dial CBV @18:53:22.50
CBV.dial SSV @18:53:23.31 Allowing 130s for 25.0ml of remaining 650.0ml @18:53:23.32 Intake.select :open @18:53:25.49 Intake @14.2psia, vacuum from 8.7 to 11.7psi @18:53:25.50 SS.reconfigure :inhaling 1.43ml/s @18:53:25.55 SS.seek 25ml @18:53:40.80 Vacuum too high -- waiting up to 40 seconds @18:53:40.81 SS.reconfigure :exhale @18:53:40.86 SS.hold @18:53:41.35 Intake @11.2psia, vacuum from 5.7 to 8.7psi @18:53:41.37 SS.reconfigure :inhaling 1.29ml/s @18:53:41.42 SS.seek 25ml @18:53:41.52 Vacuum too high -- waiting up to 40 seconds @18:53:41.53 SS.reconfigure :exhale @18:53:41.59 SS.hold @18:53:51.36 Intake @12.7psia, vacuum from 7.2 to 10.2psi @18:53:51.38 SS.reconfigure :inhaling 1.16ml/s @18:53:51.43 SS.seek 25ml @18:53:56.94 Stroke completed -- waiting for pressure to equalize ... @18:53:56.95 SS.reconfigure :exhale @18:53:57.00 SS.hold @18:54:03.42 Intake.select :close @18:54:03.57 SSV.dial :exhaust @18:54:04.34 Exhaust.select :open @18:54:04.45 SS.seek 0ml @18:54:13.66 Exhaust.select :close @18:54:13.77 SSV.dial CBV @18:54:13.79
CBV.dial SSV @18:54:14.60 Allowing 130s for 25.0ml of remaining 625.0ml @18:54:14.61 Intake.select :open @18:54:16.77 Intake @14.3psia, vacuum from 8.8 to 11.8psi @18:54:16.79 SS.reconfigure :inhaling 1.16ml/s @18:54:16.84 SS.seek 25ml @18:54:33.86 Vacuum too high -- waiting up to 40 seconds @18:54:33.87 SS.reconfigure :exhale @18:54:33.92 SS.hold @18:54:38.37 Intake @12.3psia, vacuum from 6.8 to 9.8psi @18:54:38.39 SS.reconfigure :inhaling 1.04ml/s @18:54:38.44 SS.seek 25ml @18:54:45.61 Stroke completed -- waiting for pressure to equalize ... @18:54:45.63 SS.reconfigure :exhale @18:54:45.67 SS.hold @18:54:55.46 Intake.select :close @18:54:55.61 SSV.dial :exhaust @18:54:56.41 Exhaust.select :open @18:54:56.52 SS.seek 0ml @18:55:06.57 Exhaust.select :close @18:55:06.68 SSV.dial CBV @18:55:06.71
CBV.dial SSV @18:55:07.51 Allowing 130s for 25.0ml of remaining 600.0ml @18:55:07.52 Intake.select :open @18:55:09.68 Intake @14.2psia, vacuum from 8.7 to 11.7psi @18:55:09.70 SS.reconfigure :inhaling 1.04ml/s @18:55:09.75 SS.seek 25ml @18:55:26.23 Vacuum too high -- waiting up to 40 seconds @18:55:26.24 SS.reconfigure :exhale @18:55:26.30 SS.hold @18:55:30.75 Intake @12.5psia, vacuum from 7.0 to 10.0psi @18:55:30.77 SS.reconfigure :inhaling 0.94ml/s @18:55:30.82 SS.seek 25ml @18:55:41.10 Stroke completed -- waiting for pressure to equalize ... @18:55:41.11 SS.reconfigure :exhale @18:55:41.16 SS.hold @18:55:49.72 Intake.select :close @18:55:49.88 SSV.dial :exhaust @18:55:50.66 Exhaust.select :open @18:55:50.77 SS.seek 0ml @18:55:59.65 Exhaust.select :close @18:55:59.76 SSV.dial CBV @18:55:59.78
CBV.dial SSV @18:56:00.58 Allowing 130s for 25.0ml of remaining 575.0ml @18:56:00.60 Intake.select :open @18:56:02.76 Intake @14.7psia, vacuum from 9.2 to 12.2psi @18:56:02.78 SS.reconfigure :inhaling 0.94ml/s @18:56:02.83 SS.seek 25ml @18:56:18.51 Vacuum too high -- waiting up to 40 seconds @18:56:18.53 SS.reconfigure :exhale @18:56:18.58 SS.hold @18:56:20.96 Intake @12.5psia, vacuum from 7.0 to 10.0psi @18:56:20.98 SS.reconfigure :inhaling 0.85ml/s @18:56:21.03 SS.seek 25ml @18:56:33.04 Vacuum too high -- waiting up to 40 seconds @18:56:33.06 SS.reconfigure :exhale @18:56:33.11 SS.hold @18:56:39.76 Intake @13.0psia, vacuum from 7.5 to 10.5psi @18:56:39.78 SS.reconfigure :inhaling 0.77ml/s @18:56:39.83 SS.seek 25ml @18:56:42.03 Stroke completed -- waiting for pressure to equalize ... @18:56:42.04 SS.reconfigure :exhale @18:56:42.09 SS.hold @18:56:46.26 Intake.select :close @18:56:46.42 SSV.dial :exhaust @18:56:47.20 Exhaust.select :open @18:56:47.33 SS.seek 0ml @18:56:56.54 Exhaust.select :close @18:56:56.65 SSV.dial CBV @18:56:56.67
CBV.dial SSV @18:56:57.47 Allowing 130s for 25.0ml of remaining 550.0ml @18:56:57.49 Intake.select :open @18:56:59.66 Intake @14.5psia, vacuum from 9.0 to 12.0psi @18:56:59.67 SS.reconfigure :inhaling 0.77ml/s @18:56:59.72 SS.seek 25ml @18:57:26.76 Vacuum too high -- waiting up to 40 seconds @18:57:26.78 SS.reconfigure :exhale @18:57:26.83 SS.hold @18:57:31.84 Intake @13.3psia, vacuum from 7.8 to 10.8psi @18:57:31.86 SS.reconfigure :inhaling 0.70ml/s @18:57:31.90 SS.seek 25ml @18:57:39.71 Stroke completed -- waiting for pressure to equalize ... @18:57:39.72 SS.reconfigure :exhale @18:57:39.76 SS.hold @18:57:47.24 Intake.select :close @18:57:47.40 SSV.dial :exhaust @18:57:48.18 Exhaust.select :open @18:57:48.30 SS.seek 0ml @18:57:57.53 Exhaust.select :close @18:57:57.64 SSV.dial CBV @18:57:57.67
CBV.dial SSV @18:57:58.47 Allowing 130s for 25.0ml of remaining 525.0ml @18:57:58.48 Intake.select :open @18:58:00.65 Intake @14.7psia, vacuum from 9.2 to 12.2psi @18:58:00.66 SS.reconfigure :inhaling 0.70ml/s @18:58:00.71 SS.seek 25ml @18:58:37.99 Stroke completed -- waiting for pressure to equalize ... @18:58:38.00 SS.reconfigure :exhale @18:58:38.04 SS.hold @18:58:51.06 Intake.select :close @18:58:51.29 SSV.dial :exhaust @18:58:52.07 Exhaust.select :open @18:58:52.18 SS.seek 0ml @18:59:01.03 Exhaust.select :close @18:59:01.14 SSV.dial CBV @18:59:01.18
CBV.dial SSV @18:59:01.94 Allowing 130s for 25.0ml of remaining 500.0ml @18:59:01.95 Intake.select :open @18:59:04.11 Intake @14.7psia, vacuum from 9.2 to 12.2psi @18:59:04.13 SS.reconfigure :inhaling 0.70ml/s @18:59:04.18 SS.seek 25ml @18:59:32.32 Vacuum too high -- waiting up to 40 seconds @18:59:32.34 SS.reconfigure :exhale @18:59:32.39 SS.hold @18:59:38.14 Intake @13.5psia, vacuum from 8.0 to 11.0psi @18:59:38.16 SS.reconfigure :inhaling 0.62ml/s @18:59:38.21 SS.seek 25ml @18:59:48.66 Stroke completed -- waiting for pressure to equalize ... @18:59:48.67 SS.reconfigure :exhale @18:59:48.71 SS.hold @18:59:58.56 Intake.select :close @18:59:58.71 SSV.dial :exhaust @18:59:59.49 Exhaust.select :open @18:59:59.60 SS.seek 0ml @19:00:08.91 Exhaust.select :close @19:00:09.02 SSV.dial CBV @19:00:09.05
CBV.dial SSV @19:00:09.83 Allowing 130s for 25.0ml of remaining 475.0ml @19:00:09.84 Intake.select :open @19:00:12.00 Intake @14.8psia, vacuum from 9.3 to 12.3psi @19:00:12.02 SS.reconfigure :inhaling 0.62ml/s @19:00:12.07 SS.seek 25ml @19:00:46.85 Vacuum too high -- waiting up to 40 seconds @19:00:46.87 SS.reconfigure :exhale @19:00:46.92 SS.hold @19:00:53.55 Intake @13.8psia, vacuum from 8.3 to 11.3psi @19:00:53.56 SS.reconfigure :inhaling 0.56ml/s @19:00:53.61 SS.seek 25ml @19:01:01.19 Stroke completed -- waiting for pressure to equalize ... @19:01:01.20 SS.reconfigure :exhale @19:01:01.24 SS.hold @19:01:10.03 Intake.select :close @19:01:10.19 SSV.dial :exhaust @19:01:10.95 Exhaust.select :open @19:01:11.06 SS.seek 0ml @19:01:19.95 Exhaust.select :close @19:01:20.06 SSV.dial CBV @19:01:20.09
CBV.dial SSV @19:01:20.88 Allowing 130s for 25.0ml of remaining 450.0ml @19:01:20.89 Intake.select :open @19:01:23.06 Intake @14.9psia, vacuum from 9.4 to 12.4psi @19:01:23.07 SS.reconfigure :inhaling 0.56ml/s @19:01:23.12 SS.seek 25ml @19:02:08.44 Vacuum too high -- waiting up to 40 seconds @19:02:08.46 SS.reconfigure :exhale @19:02:08.51 SS.hold @19:02:14.69 Intake @13.5psia, vacuum from 8.0 to 11.0psi @19:02:14.71 SS.reconfigure :inhaling 0.51ml/s @19:02:14.76 SS.seek 25ml @19:02:15.09 Stroke completed -- waiting for pressure to equalize ... @19:02:15.10 SS.reconfigure :exhale @19:02:15.15 SS.hold @19:02:24.93 Intake.select :close @19:02:25.09 SSV.dial :exhaust @19:02:25.86 Exhaust.select :open @19:02:25.97 SS.seek 0ml @19:02:34.94 Exhaust.select :close @19:02:35.05 SSV.dial CBV @19:02:35.07
CBV.dial SSV @19:02:35.87 Allowing 130s for 25.0ml of remaining 425.0ml @19:02:35.88 Intake.select :open @19:02:38.05 Intake @14.9psia, vacuum from 9.4 to 12.4psi @19:02:38.07 SS.reconfigure :inhaling 0.51ml/s @19:02:38.12 SS.seek 25ml @19:03:23.57 Vacuum too high -- waiting up to 40 seconds @19:03:23.58 SS.reconfigure :exhale @19:03:23.63 SS.hold @19:03:29.02 Intake @13.7psia, vacuum from 8.2 to 11.2psi @19:03:29.04 SS.reconfigure :inhaling 0.46ml/s @19:03:29.09 SS.seek 25ml @19:03:35.42 Stroke completed -- waiting for pressure to equalize ... @19:03:35.43 SS.reconfigure :exhale @19:03:35.48 SS.hold @19:03:45.25 Intake.select :close @19:03:45.41 SSV.dial :exhaust @19:03:46.19 Exhaust.select :open @19:03:46.30 SS.seek 0ml @19:03:55.61 Exhaust.select :close @19:03:55.72 SSV.dial CBV @19:03:55.74
CBV.dial SSV @19:03:56.53 Allowing 130s for 25.0ml of remaining 400.0ml @19:03:56.54 Intake.select :open @19:03:58.70 Intake @14.9psia, vacuum from 9.4 to 12.4psi @19:03:59.07 SS.reconfigure :inhaling 0.46ml/s @19:03:59.19 SS.seek 25ml @19:04:44.82 Vacuum too high -- waiting up to 40 seconds @19:04:44.83 SS.reconfigure :exhale @19:04:44.88 SS.hold @19:04:49.84 Intake @13.8psia, vacuum from 8.3 to 11.3psi @19:04:49.86 SS.reconfigure :inhaling 0.42ml/s @19:04:49.91 SS.seek 25ml @19:05:01.10 Stroke completed -- waiting for pressure to equalize ... @19:05:01.11 SS.reconfigure :exhale @19:05:01.16 SS.hold @19:05:12.65 Intake.select :close @19:05:12.80 SSV.dial :exhaust @19:05:13.59 Exhaust.select :open @19:05:13.71 SS.seek 0ml @19:05:23.06 Exhaust.select :close @19:05:23.17 SSV.dial CBV @19:05:23.19
CBV.dial SSV @19:05:24.00 Allowing 130s for 25.0ml of remaining 375.0ml @19:05:24.01 Intake.select :open @19:05:26.18 Intake @15.1psia, vacuum from 9.6 to 12.6psi @19:05:26.19 SS.reconfigure :inhaling 0.42ml/s @19:05:26.24 SS.seek 25ml @19:06:27.34 Stroke completed -- waiting for pressure to equalize ... @19:06:27.35 SS.reconfigure :exhale @19:06:27.39 SS.hold @19:06:43.90 Intake.select :close @19:06:44.06 SSV.dial :exhaust @19:06:44.84 Exhaust.select :open @19:06:44.95 SS.seek 0ml @19:06:54.35 Exhaust.select :close @19:06:54.46 SSV.dial CBV @19:06:55.03
CBV.dial SSV @19:06:55.79 Allowing 130s for 25.0ml of remaining 350.0ml @19:06:55.80 Intake.select :open @19:06:57.97 Intake @15.2psia, vacuum from 9.7 to 12.7psi @19:06:57.98 SS.reconfigure :inhaling 0.42ml/s @19:06:58.03 SS.seek 25ml @19:07:59.10 Stroke completed -- waiting for pressure to equalize ... @19:07:59.11 SS.reconfigure :exhale @19:07:59.16 SS.hold @19:08:19.57 Intake.select :close @19:08:19.72 SSV.dial :exhaust @19:08:20.50 Exhaust.select :open @19:08:20.61 SS.seek 0ml @19:08:30.05 Exhaust.select :close @19:08:30.16 SSV.dial CBV @19:08:30.18
CBV.dial SSV @19:08:30.99 Allowing 130s for 25.0ml of remaining 325.0ml @19:08:31.00 Intake.select :open @19:08:33.51 Intake @15.3psia, vacuum from 9.8 to 12.8psi @19:08:33.60 SS.reconfigure :inhaling 0.42ml/s @19:08:33.65 SS.seek 25ml @19:09:32.31 Vacuum too high -- waiting up to 40 seconds @19:09:32.32 SS.reconfigure :exhale @19:09:32.38 SS.hold @19:09:42.30 Intake @14.7psia, vacuum from 9.2 to 12.2psi @19:09:42.33 SS.reconfigure :inhaling 0.38ml/s @19:09:42.38 SS.seek 25ml @19:09:45.41 Stroke completed -- waiting for pressure to equalize ... @19:09:45.42 SS.reconfigure :exhale @19:09:45.47 SS.hold @19:09:58.57 Intake.select :close @19:09:58.73 SSV.dial :exhaust @19:09:59.51 Exhaust.select :open @19:09:59.62 SS.seek 0ml @19:10:09.11 Exhaust.select :close @19:10:09.22 SSV.dial CBV @19:10:09.25
CBV.dial SSV @19:10:10.04 Allowing 130s for 25.0ml of remaining 300.0ml @19:10:10.05 Intake.select :open @19:10:12.22 Intake @15.3psia, vacuum from 9.8 to 12.8psi @19:10:12.23 SS.reconfigure :inhaling 0.38ml/s @19:10:12.28 SS.seek 25ml @19:11:14.96 Vacuum too high -- waiting up to 40 seconds @19:11:14.98 SS.reconfigure :exhale @19:11:15.03 SS.hold @19:11:23.70 Intake @14.6psia, vacuum from 9.1 to 12.1psi @19:11:23.72 SS.reconfigure :inhaling 0.33ml/s @19:11:23.77 SS.seek 25ml @19:11:30.67 Stroke completed -- waiting for pressure to equalize ... @19:11:30.69 SS.reconfigure :exhale @19:11:31.09 SS.hold @19:11:42.66 Intake.select :close @19:11:42.82 SSV.dial :exhaust @19:11:43.60 Exhaust.select :open @19:11:43.71 SS.seek 0ml @19:11:53.19 Exhaust.select :close @19:11:53.30 SSV.dial CBV @19:11:53.33
CBV.dial SSV @19:11:54.13 Allowing 130s for 25.0ml of remaining 275.0ml @19:11:54.14 Intake.select :open @19:11:56.31 Intake @15.4psia, vacuum from 9.9 to 12.9psi @19:11:56.32 SS.reconfigure :inhaling 0.33ml/s @19:11:56.37 SS.seek 25ml @19:13:14.08 Stroke completed -- waiting for pressure to equalize ... @19:13:14.09 SS.reconfigure :exhale @19:13:14.13 SS.hold @19:13:34.66 Intake.select :close @19:13:34.82 SSV.dial :exhaust @19:13:35.61 Exhaust.select :open @19:13:35.73 SS.seek 0ml @19:13:45.17 Exhaust.select :close @19:13:45.28 SSV.dial CBV @19:13:45.32
CBV.dial SSV @19:13:46.08 Allowing 130s for 25.0ml of remaining 250.0ml @19:13:46.09 Intake.select :open @19:13:48.26 Intake @15.5psia, vacuum from 10.0 to 13.0psi @19:13:48.27 SS.reconfigure :inhaling 0.33ml/s @19:13:48.32 SS.seek 25ml @19:15:06.51 Stroke completed -- waiting for pressure to equalize ... @19:15:06.52 SS.reconfigure :exhale @19:15:06.57 SS.hold @19:15:28.94 Intake.select :close @19:15:29.10 SSV.dial :exhaust @19:15:29.87 Exhaust.select :open @19:15:29.98 SS.seek 0ml @19:15:39.59 Exhaust.select :close @19:15:39.70 SSV.dial CBV @19:15:39.74
CBV.dial SSV @19:15:40.50 Allowing 130s for 25.0ml of remaining 225.0ml @19:15:40.51 Intake.select :open @19:15:42.68 Intake @15.9psia, vacuum from 10.0 to 13.0psi @19:15:42.69 SS.reconfigure :inhaling 0.33ml/s @19:15:42.74 SS.seek 25ml @19:16:54.30 Vacuum too high -- waiting up to 40 seconds @19:16:54.31 SS.reconfigure :exhale @19:16:54.37 SS.hold @19:17:03.12 Intake @14.9psia, vacuum from 9.4 to 12.4psi @19:17:03.13 SS.reconfigure :inhaling 0.30ml/s @19:17:03.18 SS.seek 25ml @19:17:10.95 Stroke completed -- waiting for pressure to equalize ... @19:17:10.96 SS.reconfigure :exhale @19:17:11.01 SS.hold @19:17:29.41 Intake.select :close @19:17:29.56 SSV.dial :exhaust @19:17:30.34 Exhaust.select :open @19:17:30.45 SS.seek 0ml @19:17:39.96 Exhaust.select :close @19:17:40.08 SSV.dial CBV @19:17:40.10
CBV.dial SSV @19:17:41.32 Allowing 130s for 25.0ml of remaining 200.0ml @19:17:41.34 Intake.select :open @19:17:43.51 Intake @15.4psia, vacuum from 9.9 to 12.9psi @19:17:43.52 SS.reconfigure :inhaling 0.30ml/s @19:17:43.57 SS.seek 25ml @19:19:10.52 Stroke completed -- waiting for pressure to equalize ... @19:19:10.54 SS.reconfigure :exhale @19:19:10.58 SS.hold @19:19:33.31 Intake.select :close @19:19:33.46 SSV.dial :exhaust @19:19:34.24 Exhaust.select :open @19:19:34.35 SS.seek 0ml @19:19:43.88 Exhaust.select :close @19:19:43.99 SSV.dial CBV @19:19:44.01
CBV.dial SSV @19:19:44.82 Allowing 130s for 25.0ml of remaining 175.0ml @19:19:44.83 Intake.select :open @19:19:47.42 Intake @15.8psia, vacuum from 10.0 to 13.0psi @19:19:47.44 SS.reconfigure :inhaling 0.30ml/s @19:19:47.49 SS.seek 25ml @19:21:09.64 Vacuum too high -- waiting up to 40 seconds @19:21:09.65 SS.reconfigure :exhale @19:21:09.71 SS.hold @19:21:21.01 Intake @15.1psia, vacuum from 9.6 to 12.6psi @19:21:21.03 SS.reconfigure :inhaling 0.28ml/s @19:21:21.08 SS.seek 25ml @19:21:27.58 Stroke completed -- waiting for pressure to equalize ... @19:21:27.59 SS.reconfigure :exhale @19:21:27.64 SS.hold @19:21:46.01 Intake.select :close @19:21:46.16 SSV.dial :exhaust @19:21:46.93 Exhaust.select :open @19:21:47.04 SS.seek 0ml @19:21:56.51 Exhaust.select :close @19:21:56.62 SSV.dial CBV @19:21:56.64
CBV.dial SSV @19:21:57.43 Allowing 130s for 25.0ml of remaining 150.0ml @19:21:57.44 Intake.select :open @19:21:59.61 Intake @16.0psia, vacuum from 10.0 to 13.0psi @19:21:59.62 SS.reconfigure :inhaling 0.28ml/s @19:21:59.67 SS.seek 25ml @19:23:36.55 Stroke completed -- waiting for pressure to equalize ... @19:23:36.56 SS.reconfigure :exhale @19:23:36.61 SS.hold @19:24:05.29 Intake.select :close @19:24:05.44 SSV.dial :exhaust @19:24:06.22 Exhaust.select :open @19:24:06.33 SS.seek 0ml @19:24:16.02 Exhaust.select :close @19:24:16.13 SSV.dial CBV @19:24:16.16
CBV.dial SSV @19:24:16.96 Allowing 130s for 25.0ml of remaining 125.0ml @19:24:16.97 Intake.select :open @19:24:19.14 Intake @16.1psia, vacuum from 10.0 to 13.0psi @19:24:19.15 SS.reconfigure :inhaling 0.28ml/s @19:24:19.21 SS.seek 25ml @19:25:55.81 Stroke completed -- waiting for pressure to equalize ... @19:25:55.82 SS.reconfigure :exhale @19:25:55.87 SS.hold @19:26:26.91 Intake.select :close @19:26:27.06 SSV.dial :exhaust @19:26:27.84 Exhaust.select :open @19:26:27.96 SS.seek 0ml @19:26:37.44 Exhaust.select :close @19:26:37.55 SSV.dial CBV @19:26:37.58
CBV.dial SSV @19:26:38.36 Allowing 130s for 25.0ml of remaining 100.0ml @19:26:38.37 Intake.select :open @19:26:40.54 Intake @15.9psia, vacuum from 10.0 to 13.0psi @19:26:40.55 SS.reconfigure :inhaling 0.28ml/s @19:26:40.60 SS.seek 25ml @19:27:47.69 Vacuum too high -- waiting up to 40 seconds @19:27:47.71 SS.reconfigure :exhale @19:27:47.76 SS.hold @19:27:59.03 Intake @15.6psia, vacuum from 10.0 to 13.0psi @19:27:59.05 SS.reconfigure :inhaling 0.25ml/s @19:27:59.10 SS.seek 25ml @19:28:33.36 Stroke completed -- waiting for pressure to equalize ... @19:28:33.37 SS.reconfigure :exhale @19:28:33.42 SS.hold @19:28:48.38 SS.reconfigure :default @19:28:48.43 SS.hold @19:28:48.50 Intake.select :close @19:28:48.65 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 925.0ml @19:28:48.67 SSV.dial :exhaust @19:28:49.87 Exhaust.select :open @19:28:49.98 SS.reconfigure :exhale @19:28:50.03 SS.seek 0ml @19:28:59.06 Exhaust.select :close @19:28:59.17 Sampled 925.0ml @19:28:59.19 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @19:28:59.20 Intake.select :close @19:28:59.26 CSV.dial :waste @19:28:59.44 SSV.dial CBV,avoiding: :exhaust @19:28:59.47 CS.seek 0ml @19:28:59.50
CBV.dial SSV @19:28:59.65 CSV.dial :air @19:29:00.39 CS.seek 10ml @19:29:01.45
CTV.dial :air @19:29:02.24 delay 15 seconds @19:29:17.26 SSV.dial :spit,avoiding: :exhaust @19:29:18.63 CTV.dial CSV,avoiding: :intake @19:29:18.67
SS.seek 0ml @19:29:18.79 SSV.dial CBV,avoiding: :exhaust @19:29:24.56 SSV.dial between CBV and plug @19:29:25.05 CBV.dial between SSV and waste2 @19:29:25.46 CSV.dial between air and CTV @19:29:25.89 CS.seek 8.202ml @19:29:30.87 CTV.dial CSV,avoiding: :air @19:29:30.90
CSV.dial CTV @19:29:31.26 CS.reconfigure :pressurize @19:29:31.31 CS.seek 0ml @19:29:49.43 BubblePoint: Intake @24.3psia, Outlet @0.6psia @19:29:49.44 CBV.dial SSV @19:29:49.79 SS.reconfigure :default @19:29:49.84 SS.seek 10ml @19:29:55.31 delay 15 seconds @19:30:10.44 Evacuated: Intake @23.9psia, Outlet @22.7psia @19:30:10.46 SSV.dial :spit,avoiding: :exhaust @19:30:11.52 SS.reconfigure :exhale @19:30:11.57 SS.seek 0ml @19:30:16.58 SS.reconfigure :fast @19:30:16.63 SS.seek 5ml @19:30:19.42 SSV.dial CBV,avoiding: :exhaust @19:30:19.45
CBV.dial SSV @19:30:20.84 CTV.dial :air,avoiding: :intake @19:30:22.23 CSV.dial CTV @19:30:22.26
SS.seek 10ml @19:30:23.48 CSV.dial :air @19:30:24.27 CS.reconfigure :fast @19:30:24.39 CS.seek 0ml @19:30:24.76
SSV.dial :spit,avoiding: :exhaust @19:30:26.13 SS.reconfigure :exhale @19:30:26.18 SS.seek 0ml @19:30:28.43 CS.stop @19:30:31.24
SS.reconfigure :fast @19:30:31.29 SS.seek 5ml @19:30:34.28 SSV.dial CBV,avoiding: :exhaust @19:30:34.31
CBV.dial SSV @19:30:36.69 SSV.dial :spit,avoiding: :exhaust @19:30:38.03 SS.reconfigure :default @19:30:38.08 SS.seek 0ml @19:30:41.17 SS.stop @19:30:41.22 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @19:30:41.29 CSV.dial :waste @19:30:41.41 SSV.dial CBV,avoiding: :exhaust @19:30:41.44
CBV.dial SSV @19:30:42.04 CS.seek 0ml @19:30:42.11 CSV.dial :air @19:30:42.83
CTV.dial :air @19:30:42.91 CS.seek 10ml @19:30:42.98
delay 15 seconds @19:30:58.00 SSV.dial :spit,avoiding: :exhaust @19:30:59.36 CTV.dial CSV,avoiding: :intake @19:30:59.40
SS.reconfigure :exhale @19:30:59.50 SS.seek 0ml @19:30:59.57 SSV.dial CBV,avoiding: :exhaust @19:31:06.96 SSV.dial between CBV and plug @19:31:07.45 CBV.dial between SSV and waste2 @19:31:07.86 CSV.dial between air and CTV @19:31:08.17 CS.seek 7.544ml @19:31:14.62 CTV.dial CSV,avoiding: :air @19:31:14.64
CSV.dial CTV @19:31:14.99 CS.reconfigure :pressurize @19:31:15.04 CS.seek 0ml @19:31:31.90 BubblePoint: Intake @24.0psia, Outlet @1.1psia @19:31:31.91 CBV.dial SSV @19:31:32.26 SS.reconfigure :default @19:31:32.31 SS.seek 10ml @19:31:37.95 delay 15 seconds @19:31:53.08 Evacuated: Intake @23.8psia, Outlet @10.7psia @19:31:53.09 SSV.dial :spit,avoiding: :exhaust @19:31:54.17 SS.reconfigure :exhale @19:31:54.22 SS.seek 0ml @19:31:59.77 SS.reconfigure :fast @19:31:59.82 SS.seek 5ml @19:32:02.56 SSV.dial CBV,avoiding: :exhaust @19:32:02.59
CBV.dial SSV @19:32:03.98 CTV.dial :air,avoiding: :intake @19:32:05.37 CSV.dial CTV @19:32:05.40
SS.seek 10ml @19:32:06.53 CSV.dial :air @19:32:07.04 CS.reconfigure :fast @19:32:07.09 CS.seek 0ml @19:32:07.89
SSV.dial :spit,avoiding: :exhaust @19:32:09.25 SS.reconfigure :exhale @19:32:09.30 SS.seek 0ml @19:32:10.62 CS.stop @19:32:14.32
SS.reconfigure :fast @19:32:14.37 SS.seek 5ml @19:32:17.33 SSV.dial CBV,avoiding: :exhaust @19:32:17.35
CBV.dial SSV @19:32:19.75 SSV.dial :spit,avoiding: :exhaust @19:32:21.09 SS.reconfigure :default @19:32:21.14 SS.seek 0ml @19:32:24.40 SS.stop @19:32:24.44 Lysing: Adding 2.0ml AqMeOH @19:32:24.45 CBV.dial between CSV and waste1,avoiding: :DBV @19:32:25.99 CTV.dial CSV @19:32:27.35 CSV.dial CSR @19:32:28.14 Pulling 1ml of CSR.AqMeOH @19:32:28.15 CSR.select :AqMeOH @19:32:28.26 CS.reconfigure :default @19:32:28.31 CS.seek 1ml @19:32:31.71 delay 5 seconds @19:32:36.72 CSR.select :air @19:32:36.90 CS.seek 6ml @19:32:50.46 delay 2 seconds @19:32:52.47 CSV.dial :waste2 @19:32:52.91 CS.reconfigure :fast @19:32:52.96 CS.seek 0ml @19:33:07.58 CSV.dial CSR @19:33:08.16 Pulling 2ml of CSR.AqMeOH @19:33:08.17 CSR.select :AqMeOH @19:33:08.28 CS.reconfigure :default @19:33:08.33 CS.seek 2ml @19:33:14.36 delay 5 seconds @19:33:19.38 CSR.select :air @19:33:19.55 CS.seek 5.001ml @19:33:28.02 delay 2 seconds @19:33:30.03 CSV.dial CTV @19:33:30.51 CS.seek 1ml @19:33:42.15 Before lysing Collection Puck Heater at 15.5C/16.7psia @19:33:42.18 Heating Puck.from 3, :sh1 in Collection Clamp to 70C for 10:00 @19:33:42.20 CH.seek 70C,8:00 @19:36:10.89 CH.hold 10:00,70C @19:36:11.09 Begun lysing Collection Puck Heater at 68C/23.3psia @19:46:11.10 Ended lysing Collection Puck Heater at 70C/19.8psia @19:46:11.11 CS.seek 0ml @19:46:14.61 CTV.dial between spare and air,avoiding: :intake @19:46:15.66 CSV.dial :air @19:46:16.11 CS.seek 5ml @19:46:29.73 CSV.dial CTV @19:46:30.18 delay 2 seconds @19:46:32.20 CSV.dial :air @19:46:32.65 delay 2 seconds @19:46:34.67 CSV.dial CTV @19:46:35.12 delay 2 seconds @19:46:37.13 CSV.dial :air @19:46:37.59 delay 2 seconds @19:46:39.61 CS.seek 0ml @19:46:53.25 Puck @30.0psia after final lysis pressurization @19:46:53.26 Recovering Lysate into Collection syringe @19:46:53.27 CSV.dial CBV @19:46:54.27 CBV.dial CSV @19:46:54.66 CS.seek 5ml @19:47:08.23 delay 5 seconds @19:47:13.24 CTV.dial :air @19:47:13.79 CS.seek 6.999ml @19:47:19.73 delay 5 seconds @19:47:24.74 CSV.dial :air2 @19:47:25.18 CS.seek 10ml @19:47:34.13 CSV.dial between air and CTV,avoiding: CBV @19:47:35.27 Lysing Complete @19:47:35.35 Storing sh1 puck in Collection Clamp to tube 2 @19:47:35.53 SC.reconfigure :tight @19:47:35.58 SC.seek 2 @19:47:39.15 SC.configure :limp @19:47:39.38 Elbow.reconfigure :tight @19:47:39.42 Elbow.seek 2 @19:47:41.12 Forearm.reconfigure :tight @19:47:41.17 Forearm.seek 2 + 800 counts @19:47:42.25 SE.reconfigure :pick @19:47:42.31 SE.seek :beamBreak @19:47:43.73 SE.configure :default @19:47:43.89 Elbow.reconfigure :limp @19:47:43.95 Forearm.reconfigure :loose @19:47:43.99 Forearm.seek 2 @19:47:44.31 Elbow.hold @19:47:44.38 Elbow.reconfigure :tight @19:47:44.48 SE.seek 17.08 Pucks below Top Plate @19:47:45.07 Forearm.force -20 @19:47:45.12 Forearm.seek 2 + 288 counts @19:47:45.46 Forearm.reconfigure :tight @19:47:45.51 Forearm.seek 2 + 800 counts @19:47:45.81 SE.seek 18.08 Pucks below Top Plate @19:47:46.44 Forearm.reconfigure :loose @19:47:46.49 Forearm.seek :retract @19:47:47.37 Elbow.seek CC @19:47:49.04 Elbow.reconfigure :loose @19:47:49.14 Forearm.seek CC @19:47:50.17 Hand.close @19:47:50.94 CC.seek :open @19:47:55.17 Forearm.seek :retract @19:47:56.14 Elbow.reconfigure :tight @19:47:56.19 Elbow.seek 2 @19:47:57.91 Elbow.reconfigure :limp @19:47:57.96 Forearm.seek 2 @19:47:59.09 Elbow.hold @19:47:59.15 Elbow.reconfigure :tight @19:47:59.20 Hand.open @19:48:00.07 Forearm.force -20 @19:48:00.11 Forearm.seek 2 + 317 counts @19:48:00.40 Forearm.reconfigure :tight @19:48:00.45 Forearm.seek 2 + 800 counts @19:48:00.78 SE.seek :down @19:48:01.77 SE.stop @19:48:01.93
Forearm.reconfigure :loose @19:48:01.98 Forearm.seek :retract @19:48:02.87 Tranferring DA @19:48:02.88 Beginning samplePassC @19:48:02.90 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:48:03.09 Elbow.seek FlushPuck::Garage @19:48:04.86 Elbow.reconfigure :loose @19:48:04.97 Forearm.seek :garage @19:48:05.98 Hand.close @19:48:06.80 Forearm.seek :retract @19:48:07.78 Elbow.reconfigure :tight @19:48:07.83 Elbow.seek CC @19:48:10.65 Elbow.reconfigure :loose @19:48:11.19 Forearm.reconfigure :tight @19:48:11.25 Forearm.seek CC @19:48:12.55 CC.seek :closed @19:48:16.57 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.96 @19:48:16.64 Hand.open @19:48:17.50 Forearm.reconfigure :loose @19:48:17.54 Forearm.seek :retract @19:48:18.67 PSV.dial PSR @19:48:20.16 Pulling 1.5ml of PSR.flush @19:48:20.17 PSR.select :flush @19:48:20.29 PS.seek 1.5ml @19:48:36.78 delay 5 seconds @19:48:41.80 PSV.dial :waste1 @19:48:42.28 PS.seek 0ml @19:48:58.46 PSV.dial PSR @19:48:58.93 PSR.select :air @19:48:59.05 PS.seek 2.5ml @19:49:25.47 PRV.dial CBV,avoiding: :ab2 @19:49:25.49
CBV.dial PRV @19:49:27.26 PSV.dial :mixloop @19:49:28.30 CTV.dial :air,avoiding: :intake @19:49:28.49 PTV.dial :PRVmixing @19:49:29.78 PS.seek 0ml @19:49:56.18 PSV.dial :air @19:49:56.64 PS.seek 2.5ml @19:50:22.93 PSV.dial :mixloop @19:50:23.52 PS.seek 0ml @19:50:49.87 PSV.dial :air @19:50:50.33 PS.seek 2.5ml @19:51:16.65 PSV.dial :mixloop @19:51:17.58 PS.seek 0ml @19:51:44.12 Garaging FlushPuck in Collection Clamp @19:51:44.25 Elbow.reconfigure :tight @19:51:44.30 Elbow.seek CC @19:51:44.47 Elbow.reconfigure :loose @19:51:44.57 Forearm.seek CC @19:51:45.49 Hand.close @19:51:46.19 CC.seek :open @19:51:50.31 Forearm.seek :retract @19:51:51.35 Elbow.reconfigure :tight @19:51:51.40 Elbow.seek :garage @19:51:54.27 Elbow.reconfigure :loose @19:51:54.37 Forearm.reconfigure :tight @19:51:54.42 Forearm.seek :garage @19:51:55.53 Hand.open @19:51:56.37 Forearm.reconfigure :loose @19:51:56.42 Forearm.seek :retract @19:51:57.44 PSV.dial :mixloop @19:51:57.53 PTV.dial :PRVmixing @19:51:57.61 PRV.dial CBV @19:51:57.63
CBV.dial PRV @19:51:57.78 CTV.dial :air,avoiding: :intake @19:51:57.85 Loading lyfil puck from tube 3 into Collection Clamp @19:51:58.09 SC.reconfigure :tight @19:51:58.14 SC.seek 3 @19:52:01.77 SC.configure :limp @19:52:01.99 Elbow.reconfigure :tight @19:52:02.04 Elbow.seek 3 @19:52:03.86 Forearm.reconfigure :tight @19:52:03.91 Forearm.seek 3 + 800 counts @19:52:05.10 SE.reconfigure :pick @19:52:05.60 SE.seek :beamBreak @19:52:08.42 SE.configure :default @19:52:08.58 Elbow.seek 3 @19:52:08.77 Forearm.seek 3 + 300 counts @19:52:09.13 Forearm.reconfigure :loose @19:52:09.18 Hand.close @19:52:09.94 SE.seek :down @19:52:10.15
Forearm.seek :retract @19:52:11.14 Elbow.seek CC @19:52:12.58 SE.stop @19:52:12.75
Elbow.reconfigure :loose @19:52:12.86 Forearm.reconfigure :tight @19:52:12.91 Forearm.seek CC @19:52:14.19 CC.seek :closed @19:52:18.20 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.13 @19:52:18.21 Hand.open @19:52:19.00 Forearm.reconfigure :loose @19:52:19.05 Forearm.seek :retract @19:52:20.02 CTV.dial CSV,avoiding: :intake @19:52:21.39 CSV.dial CTV @19:52:21.71 PS.seek 2.5ml @19:52:48.18 CS.seek 5ml @19:53:01.81 delay 8 seconds @19:53:09.83 PSV.dial :air @19:53:10.69 delay 2 seconds @19:53:12.77 PSV.dial :waste2 @19:53:13.52 PS.seek 0ml @19:53:39.90 CBV.dial between PRV and waste1 @19:53:40.24 CSV.dial :air @19:53:40.70 CS.seek 10ml @19:53:54.28 CSV.dial CTV @19:53:54.74 CS.seek 0ml @19:54:21.19 CBV.dial :waste1 @19:54:21.52 delay 5 seconds @19:54:26.54 CTV.dial :air,avoiding: :intake @19:54:27.97 Storing lyfil puck in Collection Clamp to tube 2 @19:54:28.15 SC.reconfigure :tight @19:54:28.20 SC.seek 2 @19:54:31.79 SC.configure :limp @19:54:32.00 Elbow.reconfigure :tight @19:54:32.05 Elbow.seek 2 @19:54:33.75 Forearm.reconfigure :tight @19:54:33.80 Forearm.seek 2 + 800 counts @19:54:35.08 SE.reconfigure :pick @19:54:35.14 SE.seek :beamBreak @19:54:36.40 SE.configure :default @19:54:36.56 Elbow.reconfigure :limp @19:54:36.61 Forearm.reconfigure :loose @19:54:36.66 Forearm.seek 2 @19:54:37.08 Elbow.hold @19:54:37.15 Elbow.reconfigure :tight @19:54:37.25 SE.seek 18.09 Pucks below Top Plate @19:54:37.82 Forearm.force -20 @19:54:37.87 Forearm.seek 2 + 311 counts @19:54:38.16 Forearm.reconfigure :tight @19:54:38.21 Forearm.seek 2 + 800 counts @19:54:38.98 SE.seek 19.09 Pucks below Top Plate @19:54:39.60 Forearm.reconfigure :loose @19:54:39.64 Forearm.seek :retract @19:54:40.55 Elbow.seek CC @19:54:42.15 Elbow.reconfigure :loose @19:54:42.25 Forearm.seek CC @19:54:43.27 Hand.close @19:54:44.06 CC.seek :open @19:54:48.27 Forearm.seek :retract @19:54:49.26 Elbow.reconfigure :tight @19:54:49.31 Elbow.seek 2 @19:54:51.03 Elbow.reconfigure :limp @19:54:51.08 Forearm.seek 2 @19:54:52.19 Elbow.hold @19:54:52.26 Elbow.reconfigure :tight @19:54:52.31 Hand.open @19:54:53.18 Forearm.force -20 @19:54:53.23 Forearm.seek 2 + 315 counts @19:54:53.60 Forearm.reconfigure :tight @19:54:53.65 Forearm.seek 2 + 800 counts @19:54:54.05 SE.seek :down @19:54:54.92 SE.stop @19:54:55.19
Forearm.reconfigure :loose @19:54:55.24 Forearm.seek :retract @19:54:56.24 PSV.dial :air @19:54:56.98 PS.seek 2.5ml @19:55:23.80 delay 1 second @19:55:24.82 PSV.dial :mixloop @19:55:25.28 delay 1 second @19:55:26.29 PSV.dial :air @19:55:26.76 delay 1 second @19:55:27.78 PSV.dial :mixloop @19:55:28.24 delay 1 second @19:55:29.26 PSV.dial :air @19:55:29.73 delay 1 second @19:55:30.75 PS.seek 0ml @19:55:57.12 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:55:57.31 Elbow.seek FlushPuck::Garage @19:55:59.11 Elbow.reconfigure :loose @19:55:59.21 Forearm.seek :garage @19:56:00.25 Hand.close @19:56:01.17 Forearm.seek :retract @19:56:02.29 Elbow.reconfigure :tight @19:56:02.35 Elbow.seek PC @19:56:03.65 Elbow.reconfigure :loose @19:56:03.76 Forearm.reconfigure :tight @19:56:03.81 Forearm.seek PC @19:56:05.17 PC.seek :closed @19:56:09.60 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.53 @19:56:09.66 Hand.open @19:56:10.49 Forearm.reconfigure :loose @19:56:10.54 Forearm.seek :retract @19:56:11.60 PRV.dial :air @19:56:12.05 PSV.dial :mixloop @19:56:12.64 PS.seek 0.3ml @19:56:16.52 PSV.dial PSR @19:56:17.55 Pulling 0.4ml of PSR.air @19:56:17.57 PSR.select :air @19:56:17.60 PS.seek 0.7ml @19:56:22.55 delay 3 seconds @19:56:25.58 PSR.select :air @19:56:25.60 PS.seek 2.5ml @19:56:44.83 delay 3 seconds @19:56:47.85 PSV.dial :mixloop @19:56:48.90 PTV.dial :mixing @19:56:49.63 PBV.dial :waste2 @19:56:50.62 PS.seek 0ml @19:57:16.99 Garaging FlushPuck in Processing Clamp @19:57:17.07 Elbow.reconfigure :tight @19:57:17.11 Elbow.seek PC @19:57:17.29 Elbow.reconfigure :loose @19:57:17.40 Forearm.seek PC @19:57:18.49 Hand.close @19:57:19.18 PC.seek :open @19:57:23.67 Forearm.seek :retract @19:57:24.69 Elbow.reconfigure :tight @19:57:24.74 Elbow.seek :garage @19:57:26.17 Elbow.reconfigure :loose @19:57:26.27 Forearm.reconfigure :tight @19:57:26.32 Forearm.seek :garage @19:57:27.39 Hand.open @19:57:28.24 Forearm.reconfigure :loose @19:57:28.28 Forearm.seek :retract @19:57:29.40 Loading da puck from tube 3 into Processing Clamp @19:57:29.63 SC.reconfigure :tight @19:57:29.68 SC.seek 3 @19:57:33.34 SC.configure :limp @19:57:33.56 Elbow.reconfigure :tight @19:57:33.61 Elbow.seek 3 @19:57:35.89 Forearm.reconfigure :tight @19:57:35.96 Forearm.seek 3 + 800 counts @19:57:36.99 SE.reconfigure :pick @19:57:37.05 SE.seek :beamBreak @19:57:39.97 SE.configure :default @19:57:40.13 Elbow.seek 3 @19:57:40.32 Forearm.seek 3 + 300 counts @19:57:40.61 Forearm.reconfigure :loose @19:57:40.66 Hand.close @19:57:41.45 SE.seek :down @19:57:41.65
Forearm.seek :retract @19:57:42.60 Elbow.seek PC @19:57:44.12 SE.stop @19:57:45.14
Elbow.reconfigure :loose @19:57:45.25 Forearm.reconfigure :tight @19:57:45.29 Forearm.seek PC @19:57:46.63 PC.seek :closed @19:57:51.06 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.54 @19:57:51.07 Hand.open @19:57:51.86 Forearm.reconfigure :loose @19:57:51.91 Forearm.seek :retract @19:57:53.03 Heating Puck.from 3, :da in Processing Clamp to 28C for 2:00:00 @19:57:53.04 PH.seek 28C,5:00 @19:58:36.83 PH.hold 2:00:00,28C @19:58:36.88 delay 1:30 @20:00:06.92 PSR.select :air @20:00:06.94 PSV.dial PSR @20:00:07.99 Pulling 0.5ml of PSR.wash @20:00:08.00 PSR.select :wash @20:00:08.12 PS.seek 0.5ml @20:00:14.08 delay 5 seconds @20:00:19.10 PSR.select :air @20:00:19.22 PS.seek 2ml @20:00:35.46 PTV.dial :puck @20:00:36.19 PSV.dial PTV @20:00:36.94 PBV.dial :waste1 @20:00:38.36 PS.seek 1.5ml @20:00:44.36 delay 3 seconds @20:00:47.38 PBV.dial between PSV and waste1 @20:00:47.76 PS.seek 0ml @20:01:03.90 delay 5:00 @20:06:03.91 recoverPSRreagent08 @20:06:03.92 PTV.dial :puck @20:06:04.00 PBV.dial :waste1 @20:06:04.83 delay 30 seconds @20:06:34.86 PBV.dial between PSV and waste1 @20:06:35.23 PSR.select :air @20:06:35.25 PSV.dial PSR @20:06:36.01 PS.reconfigure :fast @20:06:36.06 PS.seek 2.5ml @20:06:59.42 PSV.dial PTV @20:07:00.17 delay 5 seconds @20:07:05.19 PSV.dial PSR @20:07:05.92 delay 5 seconds @20:07:10.94 PSV.dial PTV @20:07:11.69 delay 5 seconds @20:07:16.71 PSV.dial :air @20:07:17.49 PS.seek 0ml @20:07:40.55 PTV.dial :PRVmixing,via: PRV @20:07:41.86 PSV.dial :mixloop @20:07:42.33 PRV.dial :ab1 @20:07:43.07 PS.reconfigure :slow @20:07:43.12 PS.seek 0.15ml @20:07:46.64 delay 2 seconds @20:07:48.66 CBV.dial PRV @20:07:48.69
PRV.dial CBV @20:07:49.26 PS.seek 0.34ml @20:07:53.67 delay 2 seconds @20:07:55.69 CBV.dial between PRV and PSV @20:07:56.04 PRV.dial :air @20:07:56.49 PS.seek 0.9ml @20:08:08.48 PRV.dial :waste2 @20:08:09.22 PS.seek 0.5ml @20:08:17.95 addPMLReagent08 @20:08:17.96 PSV.dial :air @20:08:18.43 PS.reconfigure :default @20:08:18.48 PS.seek 2ml @20:08:34.61 PSV.dial :PML @20:08:35.08 PTV.dial :mixing @20:08:35.81 PBV.dial :waste1 @20:08:36.26 PS.seek 1.85ml @20:08:38.67 PBV.dial between PSV and waste1 @20:08:39.05 PS.seek 0ml @20:08:58.70 delay 2 seconds @20:09:00.72 Beginning samplePassC @20:09:00.73 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:09:00.92 Elbow.reconfigure :tight @20:09:00.97 Elbow.seek FlushPuck::Garage @20:09:02.34 Elbow.reconfigure :loose @20:09:02.88 Forearm.seek :garage @20:09:03.90 Hand.close @20:09:04.79 Forearm.seek :retract @20:09:05.79 Elbow.reconfigure :tight @20:09:05.84 Elbow.seek CC @20:09:08.61 Elbow.reconfigure :loose @20:09:08.72 Forearm.reconfigure :tight @20:09:08.77 Forearm.seek CC @20:09:10.05 CC.seek :closed @20:09:14.03 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.99 @20:09:14.10 Hand.open @20:09:14.86 Forearm.reconfigure :loose @20:09:14.91 Forearm.seek :retract @20:09:15.91 PSV.dial PSR @20:09:17.06 Pulling 1.5ml of PSR.flush @20:09:17.07 PSR.select :flush @20:09:17.20 PS.seek 1.5ml @20:09:33.42 delay 5 seconds @20:09:38.44 PSV.dial :waste1 @20:09:38.93 PS.seek 0ml @20:09:55.04 PSV.dial PSR @20:09:55.51 PSR.select :air @20:09:55.63 PS.seek 2.5ml @20:10:21.96 PRV.dial CBV,avoiding: :ab2 @20:10:21.98
CBV.dial PRV @20:10:23.62 PSV.dial :mixloop @20:10:24.66 CTV.dial :air,avoiding: :intake @20:10:24.74 PTV.dial :PRVmixing @20:10:25.49 PS.seek 0ml @20:10:52.33 PSV.dial :air @20:10:52.80 PS.seek 2.5ml @20:11:19.11 PSV.dial :mixloop @20:11:19.58 PS.seek 0ml @20:11:45.89 PSV.dial :air @20:11:46.36 PS.seek 2.5ml @20:12:12.70 PSV.dial :mixloop @20:12:13.17 PS.seek 0ml @20:12:39.61 Garaging FlushPuck in Collection Clamp @20:12:39.68 Elbow.reconfigure :tight @20:12:39.73 Elbow.seek CC @20:12:39.85 Elbow.reconfigure :loose @20:12:39.95 Forearm.seek CC @20:12:40.91 Hand.close @20:12:41.66 CC.seek :open @20:12:45.76 Forearm.seek :retract @20:12:46.74 Elbow.reconfigure :tight @20:12:46.79 Elbow.seek :garage @20:12:49.61 Elbow.reconfigure :loose @20:12:49.71 Forearm.reconfigure :tight @20:12:49.76 Forearm.seek :garage @20:12:50.87 Hand.open @20:12:51.72 Forearm.reconfigure :loose @20:12:51.77 Forearm.seek :retract @20:12:52.80 delayUntil "20:17:00.71" @20:12:52.83 Whole Cell Archive sampling 1000ml @20:12:52.85 Loading wcr puck from tube 3 into Collection Clamp @20:12:53.33 Elbow.reconfigure :tight @20:12:53.38 Elbow.seek 3 @20:12:55.33 Forearm.reconfigure :tight @20:12:55.83 Forearm.seek 3 + 800 counts @20:12:56.94 SE.reconfigure :pick @20:12:57.01 SE.seek :beamBreak @20:12:59.86 SE.configure :default @20:13:00.03 Elbow.seek 3 @20:13:00.30 Forearm.seek 3 + 300 counts @20:13:00.47 Forearm.reconfigure :loose @20:13:00.52 Hand.close @20:13:01.31 SE.seek :down @20:13:01.52 Forearm.seek :retract @20:13:02.55 Elbow.seek CC @20:13:04.13 Elbow.reconfigure :loose @20:13:04.21 SE.stop @20:13:04.31 Forearm.reconfigure :tight @20:13:04.36 Forearm.seek CC @20:13:05.71 CC.seek :closed @20:13:09.68 Collection Clamp onSpring/offSpring for 6/26 samples, on/off torque 1.96 @20:13:09.70 Hand.open @20:13:10.47 Forearm.reconfigure :loose @20:13:10.52 Forearm.seek :retract @20:13:11.56 collectSample 1000ml w/bubble point of 35psi @20:13:11.57 Priming 7ml @20:13:11.59 Intake.select :close @20:13:11.60 Exhaust.select :close @20:13:11.61 SS.hold @20:13:12.29 Calibrating pressure sensors @20:13:12.31 CTV.dial :air @20:13:12.32 SSV.dial :spit,avoiding: :exhaust @20:13:12.41 SS.reconfigure :fast @20:13:12.46 SS.seek 5ml @20:13:15.09 SSV.dial CBV @20:13:15.11 CBV.dial SSV @20:13:16.51 delay 3.8 seconds @20:13:20.44 Sensor limits: Intake max 101.1psia; Outlet max 109.4psia @20:13:20.46 SSV.dial :spit @20:13:21.80 SS.seek 0ml @20:13:24.35 SSV.dial CBV @20:13:25.71 CTV.dial :intake @20:13:26.50 SSV.dial CBV @20:13:26.53 CBV.dial SSV @20:13:26.68 Allowing 40s for 7.0ml of remaining 7.0ml @20:13:26.69 Intake.select :open @20:13:28.87 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:13:28.89 SS.reconfigure :inhaling 2.17ml/s @20:13:28.94 SS.seek 7ml @20:13:35.37 Stroke completed -- waiting for pressure to equalize ... @20:13:35.39 SS.reconfigure :exhale @20:13:35.43 SS.hold @20:13:42.40 Intake.select :close @20:13:42.57 SSV.dial :exhaust @20:13:43.35 Exhaust.select :open @20:13:43.47 SS.seek 0ml @20:13:47.32 Exhaust.select :close @20:13:47.43 Sampling 1000.0ml @20:13:47.45 Intake.select :close @20:13:47.46 Exhaust.select :close @20:13:47.47 SS.reconfigure :default @20:13:47.52 SS.hold @20:13:47.72 CTV.dial :intake @20:13:47.90 SSV.dial CBV @20:13:47.93 CBV.dial SSV @20:13:48.76 Allowing 130s for 25.0ml of remaining 1000.0ml @20:13:48.77 Intake.select :open @20:13:50.94 Intake @14.0psia, vacuum from 8.5 to 11.5psi @20:13:50.96 SS.reconfigure :inhaling 2.17ml/s @20:13:51.01 SS.seek 25ml @20:14:03.46 Vacuum too high -- waiting up to 40 seconds @20:14:03.47 SS.reconfigure :exhale @20:14:03.52 SS.hold @20:14:04.23 Intake @11.2psia, vacuum from 5.7 to 8.7psi @20:14:04.25 SS.reconfigure :inhaling 1.95ml/s @20:14:04.30 SS.seek 25ml @20:14:07.87 Stroke completed -- waiting for pressure to equalize ... @20:14:07.88 SS.reconfigure :exhale @20:14:07.93 SS.hold @20:14:21.10 Intake.select :close @20:14:21.26 SSV.dial :exhaust @20:14:22.02 Exhaust.select :open @20:14:22.14 SS.seek 0ml @20:14:31.46 Exhaust.select :close @20:14:31.57 SSV.dial CBV @20:14:31.60 CBV.dial SSV @20:14:32.40 Allowing 130s for 25.0ml of remaining 975.0ml @20:14:32.41 Intake.select :open @20:14:34.58 Intake @14.1psia, vacuum from 8.6 to 11.6psi @20:14:34.60 SS.reconfigure :inhaling 1.95ml/s @20:14:34.65 SS.seek 25ml @20:14:42.89 Vacuum too high -- waiting up to 40 seconds @20:14:42.91 SS.reconfigure :exhale @20:14:42.97 SS.hold @20:14:44.90 Intake @11.0psia, vacuum from 5.5 to 8.5psi @20:14:44.91 SS.reconfigure :inhaling 1.77ml/s @20:14:44.97 SS.seek 25ml @20:14:48.02 Vacuum too high -- waiting up to 40 seconds @20:14:48.04 SS.reconfigure :exhale @20:14:48.09 SS.hold @20:14:53.71 Intake @11.7psia, vacuum from 6.2 to 9.2psi @20:14:53.73 SS.reconfigure :inhaling 1.59ml/s @20:14:53.78 SS.seek 25ml @20:14:59.51 Vacuum too high -- waiting up to 40 seconds @20:14:59.53 SS.reconfigure :exhale @20:14:59.58 SS.hold @20:15:01.79 Intake @10.7psia, vacuum from 5.2 to 8.2psi @20:15:01.81 SS.reconfigure :inhaling 1.43ml/s @20:15:01.86 SS.seek 25ml @20:15:02.65 Stroke completed -- waiting for pressure to equalize ... @20:15:02.66 SS.reconfigure :exhale @20:15:02.71 SS.hold @20:15:05.69 Intake.select :close @20:15:05.85 SSV.dial :exhaust @20:15:06.62 Exhaust.select :open @20:15:06.74 SS.seek 0ml @20:15:15.65 Exhaust.select :close @20:15:15.76 SSV.dial CBV @20:15:15.79 CBV.dial SSV @20:15:16.60 Allowing 130s for 25.0ml of remaining 950.0ml @20:15:16.62 Intake.select :open @20:15:18.78 Intake @13.2psia, vacuum from 7.7 to 10.7psi @20:15:18.81 SS.reconfigure :inhaling 1.43ml/s @20:15:18.86 SS.seek 25ml @20:15:28.58 Vacuum too high -- waiting up to 40 seconds @20:15:28.59 SS.reconfigure :exhale @20:15:28.64 SS.hold @20:15:30.65 Intake @11.7psia, vacuum from 6.2 to 9.2psi @20:15:30.67 SS.reconfigure :inhaling 1.29ml/s @20:15:30.72 SS.seek 25ml @20:15:35.17 Vacuum too high -- waiting up to 40 seconds @20:15:35.19 SS.reconfigure :exhale @20:15:35.24 SS.hold @20:15:38.36 SS.reconfigure :inhaling 1.16ml/s @20:15:38.41 SS.seek 25ml @20:15:46.59 Stroke completed -- waiting for pressure to equalize ... @20:15:46.61 SS.reconfigure :exhale @20:15:46.65 SS.hold @20:15:52.30 Intake.select :close @20:15:52.46 SSV.dial :exhaust @20:15:53.23 Exhaust.select :open @20:15:53.34 SS.seek 0ml @20:16:02.72 Exhaust.select :close @20:16:02.84 SSV.dial CBV @20:16:02.86 CBV.dial SSV @20:16:03.66 Allowing 130s for 25.0ml of remaining 925.0ml @20:16:03.68 Intake.select :open @20:16:05.85 Intake @13.8psia, vacuum from 8.3 to 11.3psi @20:16:05.86 SS.reconfigure :inhaling 1.16ml/s @20:16:05.91 SS.seek 25ml @20:16:20.14 Vacuum too high -- waiting up to 40 seconds @20:16:20.15 SS.reconfigure :exhale @20:16:20.21 SS.hold @20:16:20.66 Intake @10.9psia, vacuum from 5.4 to 8.4psi @20:16:20.67 SS.reconfigure :inhaling 1.04ml/s @20:16:20.72 SS.seek 25ml @20:16:29.01 Vacuum too high -- waiting up to 40 seconds @20:16:29.03 SS.reconfigure :exhale @20:16:29.08 SS.hold @20:16:33.69 Intake @12.0psia, vacuum from 6.5 to 9.5psi @20:16:33.71 SS.reconfigure :inhaling 0.94ml/s @20:16:33.76 SS.seek 25ml @20:16:36.08 Stroke completed -- waiting for pressure to equalize ... @20:16:36.09 SS.reconfigure :exhale @20:16:36.14 SS.hold @20:16:39.13 Intake.select :close @20:16:39.29 SSV.dial :exhaust @20:16:40.05 Exhaust.select :open @20:16:40.17 SS.seek 0ml @20:16:49.07 Exhaust.select :close @20:16:49.18 SSV.dial CBV @20:16:49.21 CBV.dial SSV @20:16:50.04 Allowing 130s for 25.0ml of remaining 900.0ml @20:16:50.05 Intake.select :open @20:16:52.22 Intake @13.4psia, vacuum from 7.9 to 10.9psi @20:16:52.24 SS.reconfigure :inhaling 0.94ml/s @20:16:52.75 SS.seek 25ml @20:17:00.72 recoverPRVreagent08 @20:17:00.73 PTV.dial :mixing @20:17:01.48 PBV.dial :waste1 @20:17:01.87 delay 30 seconds @20:17:21.19 Stroke completed -- waiting for pressure to equalize ... @20:17:21.20 SS.reconfigure :exhale @20:17:21.25 SS.hold @20:17:27.79 Intake.select :close @20:17:27.96 SSV.dial :exhaust @20:17:28.74 Exhaust.select :open @20:17:28.85 SS.seek 0ml @20:17:31.89 PBV.dial between PSV and waste1 @20:17:32.27 PSV.dial :air @20:17:32.75 PS.reconfigure :fast @20:17:32.80 PS.seek 2.5ml @20:17:38.18 Exhaust.select :close @20:17:38.29 SSV.dial CBV @20:17:38.32 CBV.dial SSV @20:17:39.13 Allowing 130s for 25.0ml of remaining 875.0ml @20:17:39.14 Intake.select :open @20:17:41.31 Intake @13.9psia, vacuum from 8.4 to 11.4psi @20:17:41.33 SS.reconfigure :inhaling 0.94ml/s @20:17:41.38 SS.seek 25ml @20:17:56.47 PSV.dial :mixloop @20:17:56.95 delay 2 seconds @20:17:58.96 PSV.dial :air @20:17:59.45 delay 2 seconds @20:18:01.46 PSV.dial :mixloop @20:18:01.93 delay 2 seconds @20:18:02.20 Vacuum too high -- waiting up to 40 seconds @20:18:02.22 SS.reconfigure :exhale @20:18:02.28 SS.hold @20:18:02.99 Intake @11.6psia, vacuum from 6.1 to 9.1psi @20:18:03.01 SS.reconfigure :inhaling 0.85ml/s @20:18:03.07 SS.seek 25ml @20:18:03.95 PSV.dial :air @20:18:04.42 PS.seek 0ml @20:18:11.27 Stroke completed -- waiting for pressure to equalize ... @20:18:11.28 SS.reconfigure :exhale @20:18:11.33 SS.hold @20:18:16.09 Intake.select :close @20:18:16.26 SSV.dial :exhaust @20:18:17.03 Exhaust.select :open @20:18:17.16 SS.seek 0ml @20:18:26.33 Exhaust.select :close @20:18:26.44 SSV.dial CBV @20:18:26.47 CBV.dial SSV @20:18:27.27 Allowing 130s for 25.0ml of remaining 850.0ml @20:18:27.28 Intake.select :open @20:18:27.73 washCycleDA #1 of 2 @20:18:27.74 PSR.select :air @20:18:27.76 PSV.dial PSR @20:18:29.09 Pulling 0.5ml of PSR.wash @20:18:29.10 PSR.select :wash @20:18:29.66 Intake @13.8psia, vacuum from 8.3 to 11.3psi @20:18:29.68 SS.reconfigure :inhaling 0.85ml/s @20:18:29.75 SS.seek 25ml @20:18:29.77 PS.reconfigure :default @20:18:29.88 PS.seek 0.5ml @20:18:35.85 delay 5 seconds @20:18:40.87 PSR.select :air @20:18:40.99 PS.seek 2ml @20:18:57.16 PTV.dial :puck @20:18:57.90 PSV.dial PTV @20:18:58.63 PBV.dial :waste1 @20:18:59.08 PS.seek 1.5ml @20:19:00.72 Stroke completed -- waiting for pressure to equalize ... @20:19:00.73 SS.reconfigure :exhale @20:19:00.78 SS.hold @20:19:04.99 delay 3 seconds @20:19:06.43 Intake.select :close @20:19:06.60 SSV.dial :exhaust @20:19:07.37 Exhaust.select :open @20:19:07.48 SS.seek 0ml @20:19:08.01 PBV.dial between PSV and waste1 @20:19:08.40 PS.seek 0ml @20:19:16.41 Exhaust.select :close @20:19:16.52 SSV.dial CBV @20:19:16.54 CBV.dial SSV @20:19:17.36 Allowing 130s for 25.0ml of remaining 825.0ml @20:19:17.38 Intake.select :open @20:19:20.00 Intake @14.1psia, vacuum from 8.6 to 11.6psi @20:19:20.02 SS.reconfigure :inhaling 0.85ml/s @20:19:20.07 SS.seek 25ml @20:19:24.76 delay 2:00 @20:19:51.02 Stroke completed -- waiting for pressure to equalize ... @20:19:51.03 SS.reconfigure :exhale @20:19:51.08 SS.hold @20:19:57.49 Intake.select :close @20:19:57.66 SSV.dial :exhaust @20:19:58.44 Exhaust.select :open @20:19:58.55 SS.seek 0ml @20:20:07.41 Exhaust.select :close @20:20:07.52 SSV.dial CBV @20:20:07.56 CBV.dial SSV @20:20:08.35 Allowing 130s for 25.0ml of remaining 800.0ml @20:20:08.36 Intake.select :open @20:20:10.53 Intake @14.1psia, vacuum from 8.6 to 11.6psi @20:20:10.55 SS.reconfigure :inhaling 0.85ml/s @20:20:10.60 SS.seek 25ml @20:20:41.58 Stroke completed -- waiting for pressure to equalize ... @20:20:41.59 SS.reconfigure :exhale @20:20:41.64 SS.hold @20:20:49.42 Intake.select :close @20:20:49.58 SSV.dial :exhaust @20:20:50.34 Exhaust.select :open @20:20:50.46 SS.seek 0ml @20:20:59.84 Exhaust.select :close @20:20:59.96 SSV.dial CBV @20:20:59.98 CBV.dial SSV @20:21:00.78 Allowing 130s for 25.0ml of remaining 775.0ml @20:21:00.80 Intake.select :open @20:21:02.97 Intake @14.3psia, vacuum from 8.8 to 11.8psi @20:21:02.99 SS.reconfigure :inhaling 0.85ml/s @20:21:03.04 SS.seek 25ml @20:21:24.78 recoverPSRreagent08 @20:21:24.80 PTV.dial :puck @20:21:24.86 PBV.dial :waste1 @20:21:25.24 delay 30 seconds @20:21:33.62 Stroke completed -- waiting for pressure to equalize ... @20:21:33.64 SS.reconfigure :exhale @20:21:33.69 SS.hold @20:21:41.35 Intake.select :close @20:21:41.51 SSV.dial :exhaust @20:21:42.28 Exhaust.select :open @20:21:42.40 SS.seek 0ml @20:21:51.30 Exhaust.select :close @20:21:51.41 SSV.dial CBV @20:21:51.44 CBV.dial SSV @20:21:52.25 Allowing 130s for 25.0ml of remaining 750.0ml @20:21:52.27 Intake.select :open @20:21:54.44 Intake @14.8psia, vacuum from 9.3 to 12.3psi @20:21:54.45 SS.reconfigure :inhaling 0.85ml/s @20:21:54.50 SS.seek 25ml @20:21:55.26 PBV.dial between PSV and waste1 @20:21:55.64 PSR.select :air @20:21:55.66 PSV.dial PSR @20:21:56.55 PS.reconfigure :fast @20:21:56.60 PS.seek 2.5ml @20:22:20.03 PSV.dial PTV @20:22:20.77 delay 5 seconds @20:22:23.41 Vacuum too high -- waiting up to 40 seconds @20:22:23.43 SS.reconfigure :exhale @20:22:23.48 SS.hold @20:22:24.19 Intake @12.4psia, vacuum from 6.9 to 9.9psi @20:22:24.21 SS.reconfigure :inhaling 0.77ml/s @20:22:24.26 SS.seek 25ml @20:22:25.79 PSV.dial PSR @20:22:26.45 Stroke completed -- waiting for pressure to equalize ... @20:22:26.46 SS.reconfigure :exhale @20:22:26.51 SS.hold @20:22:27.05 delay 5 seconds @20:22:32.08 PSV.dial PTV @20:22:32.82 delay 5 seconds @20:22:34.34 Intake.select :close @20:22:34.51 SSV.dial :exhaust @20:22:35.28 Exhaust.select :open @20:22:35.41 SS.seek 0ml @20:22:37.83 PSV.dial :air @20:22:38.60 PS.seek 0ml @20:22:44.75 Exhaust.select :close @20:22:44.86 SSV.dial CBV @20:22:44.89 CBV.dial SSV @20:22:45.70 Allowing 130s for 25.0ml of remaining 725.0ml @20:22:45.72 Intake.select :open @20:22:47.89 Intake @14.0psia, vacuum from 8.5 to 11.5psi @20:22:47.91 SS.reconfigure :inhaling 0.77ml/s @20:22:47.95 SS.seek 25ml @20:23:02.07 washCycleDA #2 of 2 @20:23:02.08 PSR.select :air @20:23:02.10 PSV.dial PSR @20:23:03.43 Pulling 0.5ml of PSR.wash @20:23:03.44 PSR.select :wash @20:23:03.56 PS.reconfigure :default @20:23:03.61 PS.seek 0.5ml @20:23:09.57 delay 5 seconds @20:23:14.59 PSR.select :air @20:23:14.71 PS.seek 2ml @20:23:21.86 Stroke completed -- waiting for pressure to equalize ... @20:23:21.87 SS.reconfigure :exhale @20:23:21.92 SS.hold @20:23:31.38 PTV.dial :puck @20:23:31.46 PSV.dial PTV @20:23:31.62 Intake.select :close @20:23:31.78 SSV.dial :exhaust @20:23:32.21 PBV.dial :waste1 @20:23:32.57 Exhaust.select :open @20:23:32.71 PS.seek 1.5ml @20:23:32.77 SS.seek 0ml @20:23:38.69 delay 3 seconds @20:23:41.71 PBV.dial between PSV and waste1 @20:23:42.06 Exhaust.select :close @20:23:42.14 PS.seek 0ml @20:23:42.20 SSV.dial CBV @20:23:42.23 CBV.dial SSV @20:23:43.04 Allowing 130s for 25.0ml of remaining 700.0ml @20:23:43.45 Intake.select :open @20:23:45.69 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:23:45.71 SS.reconfigure :inhaling 0.77ml/s @20:23:45.76 SS.seek 25ml @20:23:58.32 delay 2:00 @20:24:19.48 Stroke completed -- waiting for pressure to equalize ... @20:24:19.49 SS.reconfigure :exhale @20:24:19.54 SS.hold @20:24:29.34 Intake.select :close @20:24:29.50 SSV.dial :exhaust @20:24:30.30 Exhaust.select :open @20:24:30.41 SS.seek 0ml @20:24:39.71 Exhaust.select :close @20:24:39.83 SSV.dial CBV @20:24:39.87 CBV.dial SSV @20:24:40.65 Allowing 130s for 25.0ml of remaining 675.0ml @20:24:40.67 Intake.select :open @20:24:42.84 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:24:42.85 SS.reconfigure :inhaling 0.77ml/s @20:24:42.90 SS.seek 25ml @20:25:13.03 Vacuum too high -- waiting up to 40 seconds @20:25:13.05 SS.reconfigure :exhale @20:25:13.11 SS.hold @20:25:16.21 Intake @13.4psia, vacuum from 7.9 to 10.9psi @20:25:16.23 SS.reconfigure :inhaling 0.70ml/s @20:25:16.28 SS.seek 25ml @20:25:21.18 Stroke completed -- waiting for pressure to equalize ... @20:25:21.19 SS.reconfigure :exhale @20:25:21.24 SS.hold @20:25:28.74 Intake.select :close @20:25:29.37 SSV.dial :exhaust @20:25:30.14 Exhaust.select :open @20:25:30.26 SS.seek 0ml @20:25:39.46 Exhaust.select :close @20:25:39.58 SSV.dial CBV @20:25:39.61 CBV.dial SSV @20:25:40.42 Allowing 130s for 25.0ml of remaining 650.0ml @20:25:40.43 Intake.select :open @20:25:42.61 Intake @15.0psia, vacuum from 9.5 to 12.5psi @20:25:42.63 SS.reconfigure :inhaling 0.70ml/s @20:25:42.68 SS.seek 25ml @20:25:58.34 recoverPSRreagent08 @20:25:58.35 PTV.dial :puck @20:25:58.42 PBV.dial :waste1 @20:25:58.82 delay 30 seconds @20:26:20.34 Stroke completed -- waiting for pressure to equalize ... @20:26:20.35 SS.reconfigure :exhale @20:26:20.40 SS.hold @20:26:28.83 PBV.dial between PSV and waste1 @20:26:29.22 PSR.select :air @20:26:29.24 PSV.dial PSR @20:26:30.01 PS.reconfigure :fast @20:26:30.06 PS.seek 2.5ml @20:26:30.69 Intake.select :close @20:26:30.85 SSV.dial :exhaust @20:26:31.62 Exhaust.select :open @20:26:31.74 SS.seek 0ml @20:26:41.12 Exhaust.select :close @20:26:41.23 SSV.dial CBV @20:26:41.26 CBV.dial SSV @20:26:42.06 Allowing 130s for 25.0ml of remaining 625.0ml @20:26:42.07 Intake.select :open @20:26:44.24 Intake @14.6psia, vacuum from 9.1 to 12.1psi @20:26:44.26 SS.reconfigure :inhaling 0.70ml/s @20:26:44.31 SS.seek 25ml @20:26:54.03 PSV.dial PTV @20:26:54.78 delay 5 seconds @20:26:59.80 PSV.dial PSR @20:27:00.56 delay 5 seconds @20:27:05.59 PSV.dial PTV @20:27:06.35 delay 5 seconds @20:27:11.37 PSV.dial :air @20:27:12.13 PS.seek 0ml @20:27:21.52 Stroke completed -- waiting for pressure to equalize ... @20:27:21.54 SS.reconfigure :exhale @20:27:21.58 SS.hold @20:27:34.28 Intake.select :close @20:27:34.45 SSV.dial :exhaust @20:27:35.21 Exhaust.select :open @20:27:35.28 getPMLReagent08 @20:27:35.30 PRV.dial :air @20:27:35.35 SS.seek 0ml @20:27:35.75 delay 5 seconds @20:27:40.77 PTV.dial :PRVmixing @20:27:42.08 delay 5 seconds @20:27:44.71 Exhaust.select :close @20:27:44.82 SSV.dial CBV @20:27:44.86 CBV.dial SSV @20:27:45.62 Allowing 130s for 25.0ml of remaining 600.0ml @20:27:45.63 Intake.select :open @20:27:47.10 PRV.dial :ab2 @20:27:47.56 PTV.dial :PRVmixing @20:27:47.64 PSV.dial :mixloop @20:27:47.80 Intake @15.2psia, vacuum from 9.7 to 12.7psi @20:27:47.82 SS.reconfigure :inhaling 0.70ml/s @20:27:47.86 SS.seek 25ml @20:27:48.12 PS.reconfigure :default @20:27:48.17 PS.seek 0.3ml @20:27:52.06 delay 2 seconds @20:27:54.08 PRV.dial :air @20:27:54.61 PS.seek 0.55ml @20:27:57.94 addPMLReagent08 @20:27:57.95 PSV.dial :air @20:27:58.43 PS.seek 2ml @20:28:14.06 PSV.dial :PML @20:28:14.53 PTV.dial :mixing @20:28:15.28 PBV.dial :waste1 @20:28:15.78 Vacuum too high -- waiting up to 40 seconds @20:28:15.80 PS.seek 1.85ml @20:28:15.82 SS.reconfigure :exhale @20:28:15.92 SS.hold @20:28:17.94 Intake @13.3psia, vacuum from 7.8 to 10.8psi @20:28:17.96 SS.reconfigure :inhaling 0.62ml/s @20:28:18.01 SS.seek 25ml @20:28:18.19 PBV.dial between PSV and waste1 @20:28:18.57 PS.seek 0ml @20:28:28.65 Stroke completed -- waiting for pressure to equalize ... @20:28:28.66 SS.reconfigure :exhale @20:28:28.71 SS.hold @20:28:38.25 delay 2 seconds @20:28:38.40 Intake.select :close @20:28:38.56 SSV.dial :exhaust @20:28:39.34 Exhaust.select :open @20:28:39.46 SS.seek 0ml @20:28:40.27 delay 8:00 @20:28:48.80 Exhaust.select :close @20:28:48.92 SSV.dial CBV @20:28:48.94 CBV.dial SSV @20:28:49.74 Allowing 130s for 25.0ml of remaining 575.0ml @20:28:49.75 Intake.select :open @20:28:52.34 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:28:52.36 SS.reconfigure :inhaling 0.62ml/s @20:28:52.41 SS.seek 25ml @20:29:34.21 Stroke completed -- waiting for pressure to equalize ... @20:29:34.22 SS.reconfigure :exhale @20:29:34.27 SS.hold @20:29:47.01 Intake.select :close @20:29:47.17 SSV.dial :exhaust @20:29:47.94 Exhaust.select :open @20:29:48.06 SS.seek 0ml @20:29:57.05 Exhaust.select :close @20:29:57.16 SSV.dial CBV @20:29:57.20 CBV.dial SSV @20:29:57.97 Allowing 130s for 25.0ml of remaining 550.0ml @20:29:57.99 Intake.select :open @20:30:00.63 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:30:00.65 SS.reconfigure :inhaling 0.62ml/s @20:30:00.70 SS.seek 25ml @20:30:32.87 Vacuum too high -- waiting up to 40 seconds @20:30:32.89 SS.reconfigure :exhale @20:30:32.94 SS.hold @20:30:35.38 Intake @13.7psia, vacuum from 8.2 to 11.2psi @20:30:35.40 SS.reconfigure :inhaling 0.56ml/s @20:30:35.45 SS.seek 25ml @20:30:46.17 Stroke completed -- waiting for pressure to equalize ... @20:30:46.19 SS.reconfigure :exhale @20:30:46.23 SS.hold @20:30:57.89 Intake.select :close @20:30:58.05 SSV.dial :exhaust @20:30:58.82 Exhaust.select :open @20:30:58.93 SS.seek 0ml @20:31:08.39 Exhaust.select :close @20:31:08.51 SSV.dial CBV @20:31:08.53 CBV.dial SSV @20:31:09.33 Allowing 130s for 25.0ml of remaining 525.0ml @20:31:09.35 Intake.select :open @20:31:11.52 Intake @15.5psia, vacuum from 10.0 to 13.0psi @20:31:11.54 SS.reconfigure :inhaling 0.56ml/s @20:31:11.59 SS.seek 25ml @20:31:51.40 Vacuum too high -- waiting up to 40 seconds @20:31:51.42 SS.reconfigure :exhale @20:31:51.47 SS.hold @20:31:54.56 Intake @13.9psia, vacuum from 8.4 to 11.4psi @20:31:54.57 SS.reconfigure :inhaling 0.51ml/s @20:31:54.63 SS.seek 25ml @20:32:01.53 Stroke completed -- waiting for pressure to equalize ... @20:32:01.54 SS.reconfigure :exhale @20:32:01.59 SS.hold @20:32:12.97 Intake.select :close @20:32:13.14 SSV.dial :exhaust @20:32:13.91 Exhaust.select :open @20:32:14.02 SS.seek 0ml @20:32:23.47 Exhaust.select :close @20:32:23.58 SSV.dial CBV @20:32:23.62 CBV.dial SSV @20:32:24.39 Allowing 130s for 25.0ml of remaining 500.0ml @20:32:24.41 Intake.select :open @20:32:26.58 Intake @15.7psia, vacuum from 10.0 to 13.0psi @20:32:26.60 SS.reconfigure :inhaling 0.51ml/s @20:32:26.65 SS.seek 25ml @20:33:15.99 Vacuum too high -- waiting up to 40 seconds @20:33:16.01 SS.reconfigure :exhale @20:33:16.06 SS.hold @20:33:19.88 Intake @14.3psia, vacuum from 8.8 to 11.8psi @20:33:19.90 SS.reconfigure :inhaling 0.46ml/s @20:33:19.95 SS.seek 25ml @20:33:21.62 Stroke completed -- waiting for pressure to equalize ... @20:33:21.63 SS.reconfigure :exhale @20:33:21.68 SS.hold @20:33:32.88 Intake.select :close @20:33:33.05 SSV.dial :exhaust @20:33:33.81 Exhaust.select :open @20:33:33.93 SS.seek 0ml @20:33:42.92 Exhaust.select :close @20:33:43.04 SSV.dial CBV @20:33:43.53 CBV.dial SSV @20:33:44.33 Allowing 130s for 25.0ml of remaining 475.0ml @20:33:44.34 Intake.select :open @20:33:46.52 Intake @15.8psia, vacuum from 10.0 to 13.0psi @20:33:46.54 SS.reconfigure :inhaling 0.46ml/s @20:33:46.63 SS.seek 25ml @20:34:42.19 Stroke completed -- waiting for pressure to equalize ... @20:34:42.20 SS.reconfigure :exhale @20:34:42.25 SS.hold @20:35:00.54 Intake.select :close @20:35:00.70 SSV.dial :exhaust @20:35:01.94 Exhaust.select :open @20:35:02.05 SS.seek 0ml @20:35:11.05 Exhaust.select :close @20:35:11.16 SSV.dial CBV @20:35:11.19 CBV.dial SSV @20:35:12.00 Allowing 130s for 25.0ml of remaining 450.0ml @20:35:12.01 Intake.select :open @20:35:14.18 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:35:14.20 SS.reconfigure :inhaling 0.46ml/s @20:35:14.25 SS.seek 25ml @20:35:51.93 Vacuum too high -- waiting up to 40 seconds @20:35:51.95 SS.reconfigure :exhale @20:35:52.05 SS.hold @20:35:57.04 Intake @15.3psia, vacuum from 9.8 to 12.8psi @20:35:57.06 SS.reconfigure :inhaling 0.42ml/s @20:35:57.11 SS.seek 25ml @20:36:17.21 Stroke completed -- waiting for pressure to equalize ... @20:36:17.23 SS.reconfigure :exhale @20:36:17.28 SS.hold @20:36:33.76 Intake.select :close @20:36:33.93 SSV.dial :exhaust @20:36:34.70 Exhaust.select :open @20:36:34.82 SS.seek 0ml @20:36:40.29 recoverPRVreagent08 @20:36:40.30 PTV.dial :mixing @20:36:40.46 PBV.dial :waste1 @20:36:40.85 delay 30 seconds @20:36:44.15 Exhaust.select :close @20:36:44.27 SSV.dial CBV @20:36:44.30 CBV.dial SSV @20:36:45.09 Allowing 130s for 25.0ml of remaining 425.0ml @20:36:45.10 Intake.select :open @20:36:47.28 Intake @15.8psia, vacuum from 10.0 to 13.0psi @20:36:47.29 SS.reconfigure :inhaling 0.42ml/s @20:36:47.34 SS.seek 25ml @20:37:10.87 PBV.dial between PSV and waste1 @20:37:11.26 PSV.dial :air @20:37:11.73 PS.reconfigure :fast @20:37:11.78 PS.seek 2.5ml @20:37:31.61 Vacuum too high -- waiting up to 40 seconds @20:37:31.63 SS.reconfigure :exhale @20:37:31.68 SS.hold @20:37:35.30 PSV.dial :mixloop @20:37:35.77 delay 2 seconds @20:37:37.76 Intake @15.5psia, vacuum from 10.0 to 13.0psi @20:37:37.78 SS.reconfigure :inhaling 0.38ml/s @20:37:37.82 SS.seek 25ml @20:37:37.88 PSV.dial :air @20:37:38.35 delay 2 seconds @20:37:40.37 PSV.dial :mixloop @20:37:40.84 delay 2 seconds @20:37:42.86 PSV.dial :air @20:37:43.33 PS.seek 0ml @20:37:57.18 Stroke completed -- waiting for pressure to equalize ... @20:37:57.19 SS.reconfigure :exhale @20:37:57.24 SS.hold @20:38:06.55 washCycleDA #1 of 3 @20:38:06.57 PSR.select :air @20:38:06.58 PSV.dial PSR @20:38:07.91 Pulling 0.5ml of PSR.wash @20:38:07.92 PSR.select :wash @20:38:08.04 PS.reconfigure :default @20:38:08.09 PS.seek 0.5ml @20:38:13.99 delay 5 seconds @20:38:19.01 PSR.select :air @20:38:19.13 PS.seek 2ml @20:38:23.88 Intake.select :close @20:38:24.05 SSV.dial :exhaust @20:38:24.82 Exhaust.select :open @20:38:24.94 SS.seek 0ml @20:38:34.43 Exhaust.select :close @20:38:34.55 SSV.dial CBV @20:38:34.57 CBV.dial SSV @20:38:35.33 PTV.dial :puck @20:38:35.41 Allowing 130s for 25.0ml of remaining 400.0ml @20:38:35.42 Intake.select :open @20:38:36.09 PSV.dial PTV @20:38:36.84 PBV.dial :waste1 @20:38:37.76 PS.seek 1.5ml @20:38:37.87 Intake @16.0psia, vacuum from 10.0 to 13.0psi @20:38:37.89 SS.reconfigure :inhaling 0.38ml/s @20:38:37.94 SS.seek 25ml @20:38:43.77 delay 3 seconds @20:38:46.79 PBV.dial between PSV and waste1 @20:38:47.19 PS.seek 0ml @20:39:03.34 delay 2:00 @20:39:46.46 Stroke completed -- waiting for pressure to equalize ... @20:39:46.47 SS.reconfigure :exhale @20:39:46.52 SS.hold @20:40:26.72 SS.reconfigure :default @20:40:26.77 SS.hold @20:40:26.85 Intake.select :close @20:40:27.01 ShallowSampler::Clogged in WCR -- Intake @16psia & Outlet @14psia won't equalize after 625.0ml @20:40:27.02 SSV.dial :exhaust @20:40:27.79 Exhaust.select :open @20:40:27.90 SS.reconfigure :exhale @20:40:27.96 SS.seek 0ml @20:40:37.45 Exhaust.select :close @20:40:37.56 Sampled 625.0ml @20:40:37.58 Drying puck before fixing for 4:00 @20:40:37.59 CBV.dial :waste2b @20:40:38.34 delay 1:00 @20:41:03.36 recoverPSRreagent08 @20:41:03.37 PTV.dial :puck @20:41:03.44 PBV.dial :waste1 @20:41:03.84 delay 30 seconds @20:41:33.86 PBV.dial between PSV and waste1 @20:41:34.26 PSR.select :air @20:41:34.28 PSV.dial PSR @20:41:35.03 PS.reconfigure :fast @20:41:35.08 PS.seek 2.5ml @20:41:38.36 CSV.dial :air @20:41:38.81 CS.reconfigure :fast @20:41:38.87 CS.seek 10ml @20:41:58.52 PSV.dial PTV @20:41:59.27 delay 5 seconds @20:42:02.78 Pumping wcr puck from tube 3 up to 35psi... @20:42:02.80 CBV.dial SSV @20:42:04.07 CBV.dial between CSV and waste2b @20:42:04.29 PSV.dial PSR @20:42:05.06 delay 5 seconds @20:42:05.55 CSV.dial between air and CTV @20:42:05.89 CS.seek 6.607ml @20:42:10.55 PSV.dial PTV @20:42:11.30 delay 5 seconds @20:42:14.62 CTV.dial CSV,avoiding: :air @20:42:14.65 CSV.dial CTV @20:42:15.50 CS.reconfigure :pressurize @20:42:15.55 CS.seek 0ml @20:42:16.32 PSV.dial :air @20:42:17.09 PS.seek 0ml @20:42:29.27 CBV.dial :waste2b @20:42:29.61 CS.reconfigure :default @20:42:29.66 CS.seek 0ml @20:42:34.09 delay 1:00 @20:42:40.27 washCycleDA #2 of 3 @20:42:40.28 PSR.select :air @20:42:40.30 PSV.dial PSR @20:42:41.63 Pulling 0.5ml of PSR.wash @20:42:41.64 PSR.select :wash @20:42:41.76 PS.reconfigure :default @20:42:41.81 PS.seek 0.5ml @20:42:47.80 delay 5 seconds @20:42:52.82 PSR.select :air @20:42:52.94 PS.seek 2ml @20:43:09.08 PTV.dial :puck @20:43:09.10 PSV.dial PTV @20:43:09.96 PBV.dial :waste1 @20:43:10.41 PS.seek 1.5ml @20:43:16.36 delay 3 seconds @20:43:19.38 PBV.dial between PSV and waste1 @20:43:19.78 PS.seek 0ml @20:43:34.11 CTV.dial :air,avoiding: :intake @20:43:35.50 Fix #1 of 1 (with RNAlater) for 4:00 @20:43:35.52 CSV.dial CSR @20:43:35.90 delay 2:00 @20:43:35.95 Pulling 2ml of CSR.RNAlater @20:43:35.96 CSR.select :RNAlater @20:43:36.08 CS.seek 2ml @20:43:41.99 delay 5 seconds @20:43:47.01 CSR.select :air @20:43:47.14 CS.reconfigure :fast @20:43:47.19 CS.seek 10ml @20:44:06.56 Pumping wcr puck from tube 3 up to 35psi... @20:44:06.58 CBV.dial SSV @20:44:07.83 CBV.dial between CSV and waste2b @20:44:08.81 CSV.dial between air and CTV @20:44:09.41 CS.seek 6.509ml @20:44:18.78 CTV.dial CSV,avoiding: :air @20:44:18.81 CSV.dial CTV @20:44:20.22 CS.reconfigure :pressurize @20:44:20.27 CS.seek 0ml @20:44:33.83 delay 4:00 @20:45:35.92 recoverPSRreagent08 @20:45:35.93 PTV.dial :puck @20:45:35.94 PBV.dial :waste1 @20:45:36.33 delay 30 seconds @20:46:06.36 PBV.dial between PSV and waste1 @20:46:06.74 PSR.select :air @20:46:06.76 PSV.dial PSR @20:46:07.49 PS.reconfigure :fast @20:46:07.55 PS.seek 2.5ml @20:46:30.96 PSV.dial PTV @20:46:31.71 delay 5 seconds @20:46:36.73 PSV.dial PSR @20:46:37.49 delay 5 seconds @20:46:42.51 PSV.dial PTV @20:46:43.25 delay 5 seconds @20:46:48.27 PSV.dial :air @20:46:49.04 PS.seek 0ml @20:47:12.27 washCycleDA #3 of 3 @20:47:12.28 PSR.select :air @20:47:12.30 PSV.dial PSR @20:47:13.63 Pulling 0.5ml of PSR.wash @20:47:13.64 PSR.select :wash @20:47:13.76 PS.reconfigure :default @20:47:13.81 PS.seek 0.5ml @20:47:19.83 delay 5 seconds @20:47:24.85 PSR.select :air @20:47:24.97 PS.seek 2ml @20:47:41.11 PTV.dial :puck @20:47:41.12 PSV.dial PTV @20:47:41.86 PBV.dial :waste1 @20:47:42.30 PS.seek 1.5ml @20:47:48.27 delay 3 seconds @20:47:51.29 PBV.dial between PSV and waste1 @20:47:51.66 PS.seek 0ml @20:48:07.80 delay 2:00 @20:48:33.86 CBV.dial :waste2b @20:48:34.21 CS.reconfigure :default @20:48:34.27 CS.seek 0ml @20:48:38.49 delay 1:00 @20:49:38.52 CTV.dial :air,avoiding: :intake @20:50:07.83 recoverPSRreagent08 @20:50:07.84 PTV.dial :puck @20:50:07.85 PBV.dial :waste1 @20:50:08.24 delay 30 seconds @20:50:38.26 PBV.dial between PSV and waste1 @20:50:38.66 PSR.select :air @20:50:38.68 PSV.dial PSR @20:50:39.43 PS.reconfigure :fast @20:50:39.48 PS.seek 2.5ml @20:51:02.86 PSV.dial PTV @20:51:03.61 delay 5 seconds @20:51:08.63 PSV.dial PSR @20:51:09.39 delay 5 seconds @20:51:14.83 PSV.dial PTV @20:51:15.64 delay 5 seconds @20:51:20.66 PSV.dial :air @20:51:21.44 PS.seek 0ml @20:51:44.73 Gate.power :camera,:ON @20:51:44.75 Mixing DA Substrates @20:51:44.76 PSR.select :air @20:51:44.77 PSV.dial PSR @20:51:46.12 Pulling 0.25ml of PSR.sub1 @20:51:46.16 PSR.select :sub1 @20:51:46.37 PS.reconfigure :default @20:51:46.51 PS.seek 0.25ml @20:51:49.89 delay 5 seconds @20:51:54.91 Pulling 0.25ml of PSR.sub2 @20:51:54.93 PSR.select :sub2 @20:51:55.05 PS.seek 0.5ml @20:51:58.36 delay 5 seconds @20:52:03.38 PSR.select :air @20:52:03.49 PS.seek 2ml @20:52:19.64 PTV.dial :puck @20:52:19.65 PSV.dial PTV @20:52:20.40 PBV.dial :waste1 @20:52:20.86 PS.seek 1.5ml @20:52:26.83 delay 3 seconds @20:52:29.85 PBV.dial between PSV and waste1 @20:52:30.22 PS.seek 0ml @20:52:46.36 delay 10 seconds @20:52:56.39 recoverPSRreagent08 @20:52:56.40 PTV.dial :puck @20:52:56.41 PBV.dial :waste1 @20:52:56.80 delay 30 seconds @20:53:26.82 PBV.dial between PSV and waste1 @20:53:27.22 PSR.select :air @20:53:27.24 PSV.dial PSR @20:53:27.99 PS.reconfigure :fast @20:53:28.04 PS.seek 2.5ml @20:53:51.61 PSV.dial PTV @20:53:52.37 delay 5 seconds @20:53:57.40 PSV.dial PSR @20:53:58.14 delay 5 seconds @20:54:03.16 PSV.dial PTV @20:54:03.90 delay 5 seconds @20:54:08.93 PSV.dial :air @20:54:09.69 PS.seek 0ml @20:54:33.05 Additional air clear @20:54:33.06 PSR.select :air @20:54:33.08 PSV.dial PSR @20:54:34.42 Pulling 2ml of PSR.air @20:54:34.43 PSR.select :air @20:54:34.45 PS.reconfigure :default @20:54:34.50 PS.seek 2ml @20:54:55.70 delay 5 seconds @20:55:00.72 PTV.dial :puck @20:55:00.74 PSV.dial PTV @20:55:01.48 PBV.dial :waste1 @20:55:01.93 PS.seek 1.5ml @20:55:07.89 delay 3 seconds @20:55:10.91 PBV.dial between PSV and waste1 @20:55:11.30 delay 1 second @20:55:12.31 PSV.dial :waste1 @20:55:13.37 PS.seek 0ml @20:55:29.60 Processing Puck Heater at 28.1C before imaging @20:55:29.61 PH.coast @20:55:29.73 Imaging da puck in Processing Clamp while storing it to tube 2 @20:55:29.90 SC.reconfigure :tight @20:55:30.44 SC.seek 2 @20:55:34.05 SC.configure :limp @20:55:34.28 Elbow.reconfigure :tight @20:55:34.33 Elbow.seek 2 @20:55:36.05 Forearm.reconfigure :tight @20:55:36.10 Forearm.seek 2 + 800 counts @20:55:37.26 SE.reconfigure :pick @20:55:37.32 SE.seek :beamBreak @20:55:38.62 SE.configure :default @20:55:38.79 Elbow.reconfigure :limp @20:55:38.84 Forearm.reconfigure :loose @20:55:38.89 Forearm.seek 2 @20:55:39.26 Elbow.hold @20:55:39.32 Elbow.reconfigure :tight @20:55:39.43 SE.seek 19.08 Pucks below Top Plate @20:55:39.99 Forearm.force -20 @20:55:40.03 Forearm.seek 2 + 291 counts @20:55:40.44 Forearm.reconfigure :tight @20:55:40.49 Forearm.seek 2 + 800 counts @20:55:40.78 SE.seek 20.08 Pucks below Top Plate @20:55:41.46 Forearm.reconfigure :loose @20:55:41.51 Forearm.seek :retract @20:55:42.42 Elbow.seek PC @20:55:44.91 Elbow.reconfigure :loose @20:55:45.01 Forearm.seek PC @20:55:46.07 Hand.close @20:55:46.87 PC.seek :open @20:55:51.47 Forearm.seek :retract @20:55:52.47 Elbow.reconfigure :tight @20:55:52.52 Elbow.seek 2 @20:55:55.60 Forearm.reconfigure :tight @20:55:55.72 Forearm.seek 2 + 800 counts @20:55:56.71 Hand.open @20:55:57.57 SE.seek 19.84 Pucks below Top Plate @20:55:57.84 SE.reconfigure :seal @20:55:57.89 SE.seek 19.18 Pucks below Top Plate @20:55:58.43 Lights, camera, ACTION! @20:55:58.47 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da25nov0520h925ml.tif @20:56:17.95 Waiting >20 seconds for da to relinquish FlushPuck @20:56:23.24 SX HX9/16 (USB:10): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21.502 seconds /var/log/esp/da25nov0520h925ml.tif: TIFF Upload Complete @20:56:23.28 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da25nov0520h925ml15s.tif @20:56:38.92 SX HX9/16 (USB:10): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da25nov0520h925ml15s.tif: TIFF Upload Complete @20:56:38.95 PuckCamera: snap -bin=2,2 21.0 /var/log/esp/midres/da25nov0520h925ml21s.tif @20:57:00.60 SX HX9/16 (USB:10): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21 seconds /var/log/esp/midres/da25nov0520h925ml21s.tif: TIFF Upload Complete @20:57:00.62 Gate.power :camera,:OFF @20:57:00.64 DAfans.email "21.502 sec auto-exposure",Subject: "da25nov0520h925ml images!" @20:57:01.08 Elbow.seek 2 @20:57:01.42 Forearm.seek 2 + 800 counts @20:57:01.51 SE.reconfigure :gently @20:57:01.58 SE.seek 19.9 Pucks below Top Plate @20:57:02.36 Elbow.reconfigure :limp @20:57:02.43 Forearm.reconfigure :loose @20:57:02.48 Forearm.seek 2 @20:57:02.89 Elbow.hold @20:57:03.01 Elbow.reconfigure :tight @20:57:03.06 SE.reconfigure :default @20:57:03.12 SE.seek 20.08 Pucks below Top Plate @20:57:03.55 Forearm.force -20 @20:57:03.61 Forearm.seek 2 + 287 counts @20:57:04.13 Forearm.reconfigure :tight @20:57:04.19 Forearm.seek 2 + 800 counts @20:57:04.71 SE.seek :down @20:57:05.54 SE.stop @20:57:05.97 Forearm.reconfigure :loose @20:57:06.09 Forearm.seek :retract @20:57:08.47 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @20:57:09.13 Elbow.seek FlushPuck::Garage @20:57:11.15 Elbow.reconfigure :loose @20:57:11.43 Forearm.seek :garage @20:57:12.55 Hand.close @20:57:13.63 Forearm.seek :retract @20:57:14.74 Elbow.reconfigure :tight @20:57:14.85 Elbow.seek PC @20:57:16.28 Elbow.reconfigure :loose @20:57:16.48 Forearm.reconfigure :tight @20:57:16.58 Forearm.seek PC @20:57:18.35 PC.seek :closed @20:57:22.95 Processing Clamp onSpring/offSpring for 12/27 samples, on/off torque 1.54 @20:57:23.08 Hand.open @20:57:24.01 Forearm.reconfigure :loose @20:57:24.11 Forearm.seek :retract @20:57:25.24 PSR.select :air @20:57:25.30 Storing wcr puck in Collection Clamp to tube 2 @20:57:25.34 PSV.dial PSR @20:57:26.08 Pulling 0.5ml of PSR.flush @20:57:26.16 PSR.select :flush @20:57:26.52 Elbow.reconfigure :tight @20:57:26.63 Elbow.seek 2 @20:57:26.70 PS.seek 0.5ml @20:57:29.53 Forearm.reconfigure :tight @20:57:29.63 Forearm.seek 2 + 800 counts @20:57:31.01 SE.reconfigure :pick @20:57:31.15 SE.seek :beamBreak @20:57:32.42 SE.configure :default @20:57:32.76 Elbow.reconfigure :limp @20:57:32.86 Forearm.reconfigure :loose @20:57:32.99 Forearm.seek 2 @20:57:33.46 Elbow.hold @20:57:33.71 Elbow.reconfigure :tight @20:57:33.85 delay 5 seconds @20:57:34.09 SE.seek 20.08 Pucks below Top Plate @20:57:34.76 Forearm.force -20 @20:57:34.85 Forearm.seek 2 + 293 counts @20:57:35.41 Forearm.reconfigure :tight @20:57:35.51 Forearm.seek 2 + 800 counts @20:57:35.89 SE.seek 21.08 Pucks below Top Plate @20:57:36.62 Forearm.reconfigure :loose @20:57:36.74 Forearm.seek :retract @20:57:37.71 Elbow.seek CC @20:57:37.99 upsync @20:57:38.91 PSR.select :air @20:57:39.03 PS.seek 2.5ml @20:57:39.52 Elbow.reconfigure :loose @20:57:39.62 Forearm.seek CC @20:57:40.58 Hand.close @20:57:41.37 CC.seek :open @20:57:46.06 Forearm.seek :retract @20:57:47.01 Elbow.reconfigure :tight @20:57:47.06 Elbow.seek 2 @20:57:48.78 Elbow.reconfigure :limp @20:57:48.84 Forearm.seek 2 @20:57:49.98 Elbow.hold @20:57:50.05 Elbow.reconfigure :tight @20:57:50.10 Hand.open @20:57:50.99 Forearm.force -20 @20:57:51.04 Forearm.seek 2 + 310 counts @20:57:51.33 Forearm.reconfigure :tight @20:57:51.38 Forearm.seek 2 + 800 counts @20:57:51.80 SE.seek :down @20:57:52.44 SE.stop @20:57:52.93 Forearm.reconfigure :loose @20:57:52.98 Forearm.seek :retract @20:57:53.96 Starting finalPurge @20:58:00.23 delay 1 second @20:58:01.25 PRV.dial :waste2,avoiding: :ab2 @20:58:03.12 PTV.dial :PRVmixing @20:58:04.42 PSV.dial :mixloop @20:58:05.46 PS.seek :empty @20:58:13.04
Waiting >20 seconds for psFlush to relinquish FlushPuck @20:58:31.85 PSR.select :air @20:58:31.87 PSV.dial PSR @20:58:32.92 Pulling 0.5ml of PSR.flush @20:58:32.93 PSR.select :flush @20:58:33.05 PS.seek 0.5ml @20:58:38.95 delay 5 seconds @20:58:43.97 PSR.select :air @20:58:44.09 PS.seek 2.5ml @20:59:05.38 delay 1 second @20:59:06.40 PBV.dial :waste1 @20:59:06.79 PTV.dial :mixing @20:59:07.54 PSV.dial :mixloop @20:59:08.59 PS.seek :empty @20:59:35.46 PSV.dial PSR @20:59:36.51 PSR.select :flush @20:59:36.64 PS.seek 1ml @20:59:47.69 PSR.select :air @20:59:47.81 PS.seek 2.5ml @21:00:03.95 PSV.dial PTV @21:00:04.70 PTV.dial :puck @21:00:05.43 PBV.dial :waste1 @21:00:05.52 PS.seek 0ml @21:00:32.01 PSV.dial PSR @21:00:32.76 PSR.select :flush @21:00:32.88 PS.seek 0.5ml @21:00:38.88 PSR.select :air @21:00:39.00 PS.seek 2.5ml @21:01:00.26 PSV.dial PTV @21:01:01.01 PBV.dial PSV @21:01:01.59 PS.seek 0ml @21:01:27.95 PSV.dial PBV @21:01:28.41 PS.seek 2.5ml @21:01:54.82 PSV.dial :waste1 @21:01:55.56 PS.seek 0ml @21:02:21.91 PSV.dial PSR @21:02:22.38 PSR.select :air @21:02:22.41 PS.seek 2.5ml @21:02:48.76 PSV.dial PTV @21:02:49.49 PBV.dial PSV @21:02:49.69 PS.seek 0ml @21:03:16.04 PSV.dial PBV @21:03:16.50 PS.seek 2.5ml @21:03:42.94 PSV.dial :waste1 @21:03:43.68 PS.seek 0ml @21:04:10.26 PSV.dial PSR @21:04:10.72 PSR.select :flush @21:04:10.85 PS.seek 1ml @21:04:21.91 PSR.select :air @21:04:22.03 PS.seek 2.5ml @21:04:38.16 PSV.dial PTV @21:04:38.90 PTV.dial :puck @21:04:38.97 PBV.dial :waste1 @21:04:39.54 PS.seek 0ml @21:05:06.04 PSV.dial PSR @21:05:06.79 PSR.select :flush @21:05:06.91 PS.seek 0.5ml @21:05:13.29 PSR.select :air @21:05:13.40 PS.seek 2.5ml @21:05:34.60 PSV.dial PTV @21:05:35.35 PBV.dial PSV @21:05:35.93 PS.seek 0ml @21:06:02.25 PSV.dial PBV @21:06:02.84 PS.seek 2.5ml @21:06:29.54 PSV.dial :waste1 @21:06:30.29 PS.seek 0ml @21:06:56.82 PSV.dial PSR @21:06:57.27 PSR.select :air @21:06:57.30 PS.seek 2.5ml @21:07:23.63 PSV.dial PTV @21:07:24.36 PBV.dial PSV @21:07:24.45 PS.seek 0ml @21:07:50.75 PSV.dial PBV @21:07:51.21 PS.seek 2.5ml @21:08:17.60 PSV.dial :waste1 @21:08:18.34 PS.seek 0ml @21:08:44.70
Loading FlushPuck into Collection Clamp from Processing Clamp @21:08:44.89 Elbow.seek PC @21:08:47.40 Elbow.reconfigure :loose @21:08:47.50 Forearm.seek PC @21:08:48.62 Hand.close @21:08:49.36 PC.seek :open @21:08:53.87 Forearm.seek :retract @21:08:54.95 Elbow.reconfigure :tight @21:08:55.00 Elbow.seek CC @21:08:58.53 Elbow.reconfigure :loose @21:08:58.63 Forearm.reconfigure :tight @21:08:58.68 Forearm.seek CC @21:08:59.97 CC.seek :closed @21:09:03.95 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.98 @21:09:04.02 Hand.open @21:09:04.80 Forearm.reconfigure :loose @21:09:04.85 Forearm.seek :retract @21:09:05.84 Flushing the Collection Valve::Manifold @21:09:05.97 CSV.dial CSR @21:09:06.41 Pulling 5ml of CSR.flush @21:09:06.42 CSR.select :flush @21:09:06.54 CS.seek 5ml @21:09:20.22 delay 5 seconds @21:09:25.24 CSR.select :air @21:09:25.41 CS.reconfigure :fast @21:09:25.46 CS.seek 7.5ml @21:09:32.07 CSV.dial :waste @21:09:33.46 CS.reconfigure :default @21:09:33.51 CS.seek 0ml @21:09:53.47 Flushing lower manifold's waste1 port @21:09:53.60 CSV.dial :flush @21:09:54.06 CS.reconfigure :fast @21:09:54.11 CS.seek 5ml @21:10:06.35 delay 5 seconds @21:10:11.37 CSV.dial :air @21:10:11.88 CS.seek 7ml @21:10:17.32 CSV.dial CTV @21:10:17.34
CTV.dial CSV @21:10:18.74 CBV.dial :waste1 @21:10:19.51 CS.seek 0ml @21:10:36.29 Flushing lower manifold's waste2 port @21:10:36.41 CSV.dial :flush @21:10:37.15 CS.seek 5ml @21:10:49.44 delay 5 seconds @21:10:54.46 CSV.dial :air @21:10:54.97 CS.seek 7ml @21:11:00.44 CSV.dial CTV @21:11:00.48
CTV.dial CSV @21:11:00.91 CBV.dial :waste2 @21:11:01.96 CS.seek 0ml @21:11:18.78 Flushing lower manifold's waste2b port @21:11:18.91 CSV.dial :flush @21:11:19.65 CS.seek 5ml @21:11:32.42 delay 5 seconds @21:11:37.44 CSV.dial :air @21:11:37.96 CS.seek 7ml @21:11:43.35 CSV.dial CTV @21:11:43.38
CTV.dial CSV @21:11:43.82 CBV.dial :waste2b @21:11:44.85 CS.seek 0ml @21:12:01.66 Disinfecting external Intake with kill @21:12:01.78 CSV.dial CSR @21:12:02.22 Pulling 8ml of CSR.kill @21:12:02.23 CSR.select :kill @21:12:02.35 CS.seek 8ml @21:12:21.47 delay 5 seconds @21:12:26.49 CSR.select :air @21:12:26.61 CS.seek 10ml @21:12:32.03 CSV.dial CBV @21:12:32.06
CBV.dial CSV @21:12:33.11 CTV.dial :intake @21:12:33.88 Intake.select :open @21:12:33.99 CS.seek 2ml @21:12:53.03 Intake.select :close @21:12:53.14 CTV.dial :air @21:12:53.91 CS.reconfigure :default @21:12:53.96 CS.seek 0ml @21:12:59.88 CSV.dial CSR @21:13:00.89 Pulling 5ml of CSR.flush @21:13:00.90 CSR.select :flush @21:13:01.02 CS.seek 5ml @21:13:14.66 delay 5 seconds @21:13:19.68 CSR.select :air @21:13:19.80 CS.seek 10ml @21:13:33.41 CSV.dial CBV @21:13:34.41 CS.seek 0ml @21:14:00.88 Garaging FlushPuck in Collection Clamp @21:14:00.95 Elbow.reconfigure :tight @21:14:00.99 Elbow.seek CC @21:14:01.11 Elbow.reconfigure :loose @21:14:01.22 Forearm.seek CC @21:14:02.22 Hand.close @21:14:02.98 CC.seek :open @21:14:07.05 Forearm.seek :retract @21:14:08.04 Elbow.reconfigure :tight @21:14:08.09 Elbow.seek :garage @21:14:10.97 Elbow.reconfigure :loose @21:14:11.07 Forearm.reconfigure :tight @21:14:11.12 Forearm.seek :garage @21:14:12.22 Hand.open @21:14:13.04 Forearm.reconfigure :loose @21:14:13.09 Forearm.seek :retract @21:14:14.05 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 18:38:44 @21:14:14.11 Asleep after completing DA_WCR_110525 assays @21:14:14.12 GoodNews.email "{1 => 22,\n 2 => 22,\n 3 => 0,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@21:08:12, 41% flow, 19.6C, 24% humidity, 14.6psia, 13.76V, 0.321A avg, 256.312Ah, 4.42W\nSampled 625.0ml",Subject: "Asleep after completing DA_WCR_110525 assays" @21:14:18.08 upsync + ESPwhich DA_WCR_111225.rb + fn=DA_WCR_111225.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_111225.rb + [ -f /home/esp/esp2/mission/DA_WCR_111225.rb ] + realpath /home/esp/esp2/mission/DA_WCR_111225.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_111225.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_111225.rb @17:45:05.71UTC12-Nov-25
ESP2 v3.42 I2C Gateway v3.20 for core bus @17:45:08.38 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @17:45:11.97 Polling Can every 10:00 @17:45:12.06 Cleanly exited at 21:14:18UTC05-Nov-25 @17:45:12.11 Loading /home/esp/esp2local/mission/DA_WCR_111225.rb mission @17:45:12.16 GoodNews.email "Starting DA_WCR_111225 w/pucks in tube 4\n{1 => 22,\n 2 => 22,\n 3 => 0,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@17:45:11, 41% flow, 18.9C, 25% humidity, 14.5psia, 13.75V, 0.233A avg, 291.543Ah, 3.21W",Subject: "DA_WCR_111225 begun" @17:45:12.19
Preparing to sleep until + 00:00:05 @17:45:12.24 Asleep til + 00:00:05 @17:45:12.26 Continuing at 17:45 @17:45:12.54 Gate.power :core,:OFF @17:45:12.56 Gate.power :raw,:OFF @17:45:12.57 Shutdown @17:45:15.11 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_111225.rb:14\n{1 => 22,\n 2 => 22,\n 3 => 0,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@17:45:12, 41% flow, 18.9C, 25% humidity, 14.5psia, 13.75V, 0.227A avg, 291.543Ah, 3.12W",Subject: "Asleep until 17:45" @17:45:16.50 upsync @17:45:17.14
Powering core bus @17:45:19.02 upsync @17:45:22.74
Gate.power :raw,:ON @17:45:23.50 CS.configure :default @17:45:23.54 PS.configure :default @17:45:23.59 SS.configure :default @17:45:23.64 CC.configure :default @17:45:23.68 PC.configure :default @17:45:23.75 Forearm.configure :loose @17:45:23.80 Elbow.configure :loose @17:45:23.86 SC.configure :loose @17:45:23.91 SE.configure :default @17:45:23.96 CH.configure :default @17:45:23.99 PH.configure :default @17:45:24.03 All dwarf controllers running firmware version 3.85 @17:45:24.14 Valve.reset @17:45:24.39 Intake.select :close @17:45:24.46 Exhaust.select :close @17:45:24.47 CTV.dial :air @17:45:24.55 PSV.dial :air @17:45:24.60 CSV.dial :waste1 @17:45:24.73
Forearm.home.seek :retract @17:45:25.10 SSV.dial :spit,avoiding: :exhaust @17:45:25.53 Hand.close @17:45:25.55
Elbow.reconfigure :loose @17:45:25.64 Elbow.jog 1500 @17:45:25.71 PS.home.seek 0ml @17:45:25.97 CS.reconfigure :fast @17:45:26.00 SS.home.seek 0ml @17:45:26.11 CS.home.seek 0ml @17:45:26.17
Elbow.reconfigure :loose @17:45:26.22 Elbow.home.seek :clear @17:45:29.39 SE.home.seek :down @17:45:30.02 SC.reconfigure :tight @17:45:30.07 SC.home.seek :initial @17:45:35.04 PS.stop @17:45:42.73
SC.configure :limp @17:45:42.78 Forearm.seek :retract @17:45:42.85 Elbow.seek :hammer @17:45:43.85 Forearm.reconfigure :tight @17:45:43.90 Forearm.seek retracted @17:45:44.54 PC.home.seek :open @17:45:44.55
CC.home.seek :open @17:45:47.67 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @17:45:47.68 Notice.email "At 17:45:47 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_111225.rb:14\n{1 => 22,\n 2 => 22,\n 3 => 0,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@17:45:47, 41% flow, 19.0C, 25% humidity, 14.5psia, 13.64V, 0.296A avg, 291.547Ah, 4.04W",Subject: "Started DA_WCR_111225 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @17:45:47.70
Starting initialPurge @17:45:47.73 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:45:47.80 Hand.open @17:45:48.72 Elbow.reconfigure :tight @17:45:48.80 Elbow.seek FlushPuck::Garage @17:45:50.64 Elbow.reconfigure :loose @17:45:50.76 Forearm.reconfigure :loose @17:45:50.81 Forearm.seek :garage @17:45:51.68 upsync @17:45:51.85
Hand.close @17:45:52.71 Forearm.seek :retract @17:45:53.73 Elbow.reconfigure :tight @17:45:53.78 Elbow.seek CC @17:45:56.63 Elbow.reconfigure :loose @17:45:56.73 Forearm.reconfigure :tight @17:45:56.78 Forearm.seek CC @17:45:58.07 CC.seek :closed @17:46:02.09 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.04 @17:46:02.15 Hand.open @17:46:02.91 Forearm.reconfigure :loose @17:46:02.95 Forearm.seek :retract @17:46:03.93 Flushing the Collection Valve::Manifold @17:46:04.05 CSV.dial CSR @17:46:05.34 Pulling 4.999ml of CSR.flush @17:46:05.35 CSR.select :flush @17:46:05.46 CS.reconfigure :default @17:46:05.51 CS.seek 5ml @17:46:19.55 delay 5 seconds @17:46:24.57 CSR.select :air @17:46:24.74 CS.reconfigure :fast @17:46:24.78 CS.seek 7.5ml @17:46:31.37 CSV.dial :waste @17:46:32.65 CS.reconfigure :default @17:46:32.70 CS.seek 0ml @17:46:52.65 Purging external Intake @17:46:52.77 CSV.dial :flush @17:46:53.20 CS.reconfigure :fast @17:46:53.25 CS.seek 10ml @17:47:17.40 delay 5 seconds @17:47:22.42 CSV.dial CBV @17:47:22.45
CBV.dial CSV @17:47:23.21 CTV.dial :intake @17:47:23.99 Intake.select :open @17:47:24.10 CS.seek 0ml @17:47:48.02 Intake.select :close @17:47:48.13 CTV.dial :air @17:47:48.98 Flushing upper manifold @17:47:49.09 CSV.dial :flush @17:47:49.83 CS.seek 5ml @17:48:02.30 delay 5 seconds @17:48:07.32 CSV.dial :air @17:48:07.82 CS.seek 7ml @17:48:13.34 CSV.dial CBV @17:48:13.36
CBV.dial CSV @17:48:14.40 CTV.dial :air @17:48:14.48 CS.seek 0ml @17:48:31.36 delay 7 seconds @17:48:38.39 CS.seek 3ml @17:48:46.21 CSV.dial :waste @17:48:46.67 CS.seek 0ml @17:48:54.72 CSV.dial :flush @17:48:55.17 CS.seek 3ml @17:49:02.99 delay 5 seconds @17:49:08.01 CSV.dial :air @17:49:08.63 CS.seek 5ml @17:49:14.21 CSV.dial :air @17:49:14.29 CS.seek 0ml @17:49:26.65 delay 7 seconds @17:49:33.67 CS.seek 3ml @17:49:41.43 CSV.dial :waste @17:49:42.15 CS.seek 0ml @17:49:49.96 Garaging FlushPuck in Collection Clamp @17:49:50.03 Elbow.reconfigure :tight @17:49:50.08 Elbow.seek CC @17:49:50.19 Elbow.reconfigure :loose @17:49:50.35 Forearm.seek CC @17:49:51.35 Hand.close @17:49:52.09 CC.seek :open @17:49:56.58 Forearm.seek :retract @17:49:57.54 Elbow.reconfigure :tight @17:49:57.59 Elbow.seek :garage @17:50:00.50 Elbow.reconfigure :loose @17:50:00.59 Forearm.reconfigure :tight @17:50:00.64 Forearm.seek :garage @17:50:01.70 Hand.open @17:50:02.52 Forearm.reconfigure :loose @17:50:02.56 Forearm.seek :retract @17:50:03.54 da sampling at most 1000ml, wcr at most 1000ml @17:50:03.58 Loading sh1 puck from tube 4 into Collection Clamp @17:50:03.60 DAfans.email "Started da in tube 4 at 17:50:03UTC12-Nov-25\n{1 => 22,\n 2 => 22,\n 3 => 0,\n 4 => 22,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@17:45:47, 41% flow, 19.0C, 25% humidity, 14.5psia, 13.64V, 0.296A avg, 291.547Ah, 4.04W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @17:50:03.87
SC.reconfigure :tight @17:50:03.93 SC.seek 4 @17:50:03.99 SC.configure :limp @17:50:04.25 Elbow.reconfigure :tight @17:50:04.31 Elbow.seek 4 @17:50:06.34 Forearm.reconfigure :tight @17:50:06.44 Forearm.seek 4 + 800 counts @17:50:07.59 SE.reconfigure :pick @17:50:07.64 SE.seek :beamBreak @17:50:07.74 upsync @17:50:08.60
SE.configure :default @17:50:08.76 Elbow.seek 4 @17:50:08.94 Forearm.seek 4 + 300 counts @17:50:09.21 Forearm.reconfigure :loose @17:50:09.26 Hand.close @17:50:09.97 SE.seek :down @17:50:10.61
Forearm.seek :retract @17:50:10.70 SE.stop @17:50:11.60
Elbow.seek CC @17:50:13.18 Elbow.reconfigure :loose @17:50:13.29 Forearm.reconfigure :tight @17:50:13.33 Forearm.seek CC @17:50:14.65 CC.seek :closed @17:50:18.62 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.98 @17:50:18.63 Hand.open @17:50:19.39 Forearm.reconfigure :loose @17:50:19.43 Forearm.seek :retract @17:50:20.43 collectSample 1000ml w/bubble point of 24psi @17:50:20.44 Priming 7ml @17:50:20.45 Intake.select :close @17:50:20.46 Exhaust.select :close @17:50:20.47 SS.hold @17:50:20.68 Calibrating pressure and temperature sensors @17:50:20.71 CTV.dial :air @17:50:20.72 SSV.dial :spit,avoiding: :exhaust @17:50:20.74 SS.reconfigure :fast @17:50:20.78 SS.seek 5ml @17:50:23.75 SSV.dial CBV @17:50:23.78
CBV.dial SSV @17:50:25.17 delay 3.8 seconds @17:50:29.09 Sensor limits: Intake max 94.5psia; Outlet max 109.5psia @17:50:29.10 SSV.dial :spit @17:50:30.46 SS.seek 0ml @17:50:33.20 SSV.dial CBV @17:50:34.55 CTV.dial :intake @17:50:35.33 SSV.dial CBV @17:50:35.36
CBV.dial SSV @17:50:35.50 Allowing 40s for 7.0ml of remaining 7.0ml @17:50:35.51 Intake.select :open @17:50:37.66 Intake @14.4psia, vacuum from 8.9 to 11.9psi @17:50:37.68 SS.reconfigure :inhaling 2.17ml/s @17:50:37.73 SS.seek 7ml @17:50:44.32 Stroke completed -- waiting for pressure to equalize ... @17:50:44.33 SS.reconfigure :exhale @17:50:44.38 SS.hold @17:50:46.81 Intake.select :close @17:50:46.96 SSV.dial :exhaust @17:50:47.73 Exhaust.select :open @17:50:47.84 SS.seek 0ml @17:50:52.12 Exhaust.select :close @17:50:52.23 Sampling 1000.0ml @17:50:52.24 Intake.select :close @17:50:52.25 Exhaust.select :close @17:50:52.26 SS.reconfigure :default @17:50:52.31 SS.hold @17:50:52.51 CTV.dial :intake @17:50:52.69 SSV.dial CBV @17:50:52.72
CBV.dial SSV @17:50:53.51 Allowing 130s for 25.0ml of remaining 1000.0ml @17:50:53.52 Intake.select :open @17:50:55.68 Intake @14.3psia, vacuum from 8.8 to 11.8psi @17:50:55.70 SS.reconfigure :inhaling 2.17ml/s @17:50:55.75 SS.seek 25ml @17:51:10.18 Stroke completed -- waiting for pressure to equalize ... @17:51:10.19 SS.reconfigure :exhale @17:51:10.23 SS.hold @17:51:18.77 Intake.select :close @17:51:18.92 SSV.dial :exhaust @17:51:19.68 Exhaust.select :open @17:51:19.79 SS.seek 0ml @17:51:29.13 Exhaust.select :close @17:51:29.24 SSV.dial CBV @17:51:29.26
CBV.dial SSV @17:51:30.07 Allowing 130s for 25.0ml of remaining 975.0ml @17:51:30.08 Intake.select :open @17:51:32.25 Intake @13.9psia, vacuum from 8.4 to 11.4psi @17:51:32.26 SS.reconfigure :inhaling 2.17ml/s @17:51:32.74 SS.seek 25ml @17:51:46.71 Stroke completed -- waiting for pressure to equalize ... @17:51:46.72 SS.reconfigure :exhale @17:51:46.77 SS.hold @17:51:55.30 Intake.select :close @17:51:55.45 SSV.dial :exhaust @17:51:56.23 Exhaust.select :open @17:51:56.34 SS.seek 0ml @17:52:05.51 Exhaust.select :close @17:52:05.62 SSV.dial CBV @17:52:05.64
CBV.dial SSV @17:52:06.45 Allowing 130s for 25.0ml of remaining 950.0ml @17:52:06.46 Intake.select :open @17:52:08.62 Intake @13.9psia, vacuum from 8.4 to 11.4psi @17:52:08.64 SS.reconfigure :inhaling 2.17ml/s @17:52:08.69 SS.seek 25ml @17:52:22.79 Stroke completed -- waiting for pressure to equalize ... @17:52:22.80 SS.reconfigure :exhale @17:52:22.84 SS.hold @17:52:32.51 Intake.select :close @17:52:32.66 SSV.dial :exhaust @17:52:33.42 Exhaust.select :open @17:52:33.53 SS.seek 0ml @17:52:42.76 Exhaust.select :close @17:52:42.87 SSV.dial CBV @17:52:42.89
CBV.dial SSV @17:52:43.70 Allowing 130s for 25.0ml of remaining 925.0ml @17:52:43.71 Intake.select :open @17:52:45.87 Intake @13.8psia, vacuum from 8.3 to 11.3psi @17:52:45.89 SS.reconfigure :inhaling 2.17ml/s @17:52:45.94 SS.seek 25ml @17:52:59.77 Vacuum too high -- waiting up to 40 seconds @17:52:59.78 SS.reconfigure :exhale @17:52:59.83 SS.hold @17:53:00.23 Intake @9.7psia, vacuum from 4.2 to 7.2psi @17:53:00.25 SS.reconfigure :inhaling 1.95ml/s @17:53:00.30 SS.seek 25ml @17:53:00.57 Stroke completed -- waiting for pressure to equalize ... @17:53:00.58 SS.reconfigure :exhale @17:53:00.62 SS.hold @17:53:09.54 Intake.select :close @17:53:09.69 SSV.dial :exhaust @17:53:10.46 Exhaust.select :open @17:53:10.57 SS.seek 0ml @17:53:19.76 Exhaust.select :close @17:53:19.87 SSV.dial CBV @17:53:19.91
CBV.dial SSV @17:53:20.66 Allowing 130s for 25.0ml of remaining 900.0ml @17:53:20.68 Intake.select :open @17:53:22.84 Intake @13.5psia, vacuum from 8.0 to 11.0psi @17:53:22.86 SS.reconfigure :inhaling 1.95ml/s @17:53:22.90 SS.seek 25ml @17:53:37.87 Stroke completed -- waiting for pressure to equalize ... @17:53:37.88 SS.reconfigure :exhale @17:53:37.92 SS.hold @17:53:47.58 Intake.select :close @17:53:47.73 SSV.dial :exhaust @17:53:48.51 Exhaust.select :open @17:53:48.62 SS.seek 0ml @17:53:57.48 Exhaust.select :close @17:53:57.58 SSV.dial CBV @17:53:57.61
CBV.dial SSV @17:53:58.40 Allowing 130s for 25.0ml of remaining 875.0ml @17:53:58.41 Intake.select :open @17:54:00.57 Intake @13.9psia, vacuum from 8.4 to 11.4psi @17:54:00.59 SS.reconfigure :inhaling 1.95ml/s @17:54:00.64 SS.seek 25ml @17:54:15.93 Stroke completed -- waiting for pressure to equalize ... @17:54:15.94 SS.reconfigure :exhale @17:54:15.98 SS.hold @17:54:26.02 Intake.select :close @17:54:26.17 SSV.dial :exhaust @17:54:26.93 Exhaust.select :open @17:54:27.04 SS.seek 0ml @17:54:35.99 Exhaust.select :close @17:54:36.10 SSV.dial CBV @17:54:36.12
CBV.dial SSV @17:54:36.93 Allowing 130s for 25.0ml of remaining 850.0ml @17:54:36.94 Intake.select :open @17:54:39.10 Intake @13.8psia, vacuum from 8.3 to 11.3psi @17:54:39.12 SS.reconfigure :inhaling 1.95ml/s @17:54:39.17 SS.seek 25ml @17:54:54.55 Stroke completed -- waiting for pressure to equalize ... @17:54:54.56 SS.reconfigure :exhale @17:54:54.61 SS.hold @17:55:04.36 Intake.select :close @17:55:04.51 SSV.dial :exhaust @17:55:05.29 Exhaust.select :open @17:55:05.41 SS.seek 0ml @17:55:14.69 Exhaust.select :close @17:55:14.80 SSV.dial CBV @17:55:14.84
CBV.dial SSV @17:55:15.60 Allowing 130s for 25.0ml of remaining 825.0ml @17:55:15.61 Intake.select :open @17:55:17.77 Intake @13.7psia, vacuum from 8.2 to 11.2psi @17:55:17.79 SS.reconfigure :inhaling 1.95ml/s @17:55:17.84 SS.seek 25ml @17:55:32.72 Stroke completed -- waiting for pressure to equalize ... @17:55:32.73 SS.reconfigure :exhale @17:55:32.78 SS.hold @17:55:43.90 Intake.select :close @17:55:44.06 SSV.dial :exhaust @17:55:44.82 Exhaust.select :open @17:55:44.93 SS.seek 0ml @17:55:54.10 Exhaust.select :close @17:55:54.58 SSV.dial CBV @17:55:54.60
CBV.dial SSV @17:55:55.40 Allowing 130s for 25.0ml of remaining 800.0ml @17:55:55.41 Intake.select :open @17:55:57.57 Intake @14.1psia, vacuum from 8.6 to 11.6psi @17:55:57.59 SS.reconfigure :inhaling 1.95ml/s @17:55:57.64 SS.seek 25ml @17:56:12.77 Stroke completed -- waiting for pressure to equalize ... @17:56:12.78 SS.reconfigure :exhale @17:56:12.82 SS.hold @17:56:25.46 Intake.select :close @17:56:25.62 SSV.dial :exhaust @17:56:26.38 Exhaust.select :open @17:56:26.49 SS.seek 0ml @17:56:35.72 Exhaust.select :close @17:56:35.83 SSV.dial CBV @17:56:35.86
CBV.dial SSV @17:56:36.64 Allowing 130s for 25.0ml of remaining 775.0ml @17:56:36.66 Intake.select :open @17:56:38.82 Intake @13.9psia, vacuum from 8.4 to 11.4psi @17:56:38.84 SS.reconfigure :inhaling 1.95ml/s @17:56:38.88 SS.seek 25ml @17:56:53.72 Stroke completed -- waiting for pressure to equalize ... @17:56:53.73 SS.reconfigure :exhale @17:56:53.77 SS.hold @17:57:06.64 Intake.select :close @17:57:06.79 SSV.dial :exhaust @17:57:07.55 Exhaust.select :open @17:57:07.66 SS.seek 0ml @17:57:16.89 Exhaust.select :close @17:57:17.00 SSV.dial CBV @17:57:17.02
CBV.dial SSV @17:57:17.82 Allowing 130s for 25.0ml of remaining 750.0ml @17:57:17.83 Intake.select :open @17:57:19.99 Intake @13.8psia, vacuum from 8.3 to 11.3psi @17:57:20.01 SS.reconfigure :inhaling 1.95ml/s @17:57:20.05 SS.seek 25ml @17:57:32.70 Vacuum too high -- waiting up to 40 seconds @17:57:32.71 SS.reconfigure :exhale @17:57:32.76 SS.hold @17:57:33.40 Intake @11.1psia, vacuum from 5.6 to 8.6psi @17:57:33.42 SS.reconfigure :inhaling 1.77ml/s @17:57:33.47 SS.seek 25ml @17:57:33.55 Vacuum too high -- waiting up to 40 seconds @17:57:33.56 SS.reconfigure :exhale @17:57:33.61 SS.hold @17:57:43.54 Intake @12.5psia, vacuum from 7.0 to 10.0psi @17:57:43.56 SS.reconfigure :inhaling 1.59ml/s @17:57:43.61 SS.seek 25ml @17:57:47.27 Stroke completed -- waiting for pressure to equalize ... @17:57:47.28 SS.reconfigure :exhale @17:57:47.32 SS.hold @17:57:50.75 Intake.select :close @17:57:50.90 SSV.dial :exhaust @17:57:51.66 Exhaust.select :open @17:57:51.77 SS.seek 0ml @17:58:00.53 Exhaust.select :close @17:58:00.64 SSV.dial CBV @17:58:00.67
CBV.dial SSV @17:58:01.46 Allowing 130s for 25.0ml of remaining 725.0ml @17:58:01.47 Intake.select :open @17:58:03.63 Intake @14.1psia, vacuum from 8.6 to 11.6psi @17:58:03.65 SS.reconfigure :inhaling 1.59ml/s @17:58:03.69 SS.seek 25ml @17:58:20.66 Vacuum too high -- waiting up to 40 seconds @17:58:20.68 SS.reconfigure :exhale @17:58:20.73 SS.hold @17:58:21.26 Intake @11.2psia, vacuum from 5.7 to 8.7psi @17:58:21.27 SS.reconfigure :inhaling 1.43ml/s @17:58:21.32 SS.seek 25ml @17:58:23.09 Stroke completed -- waiting for pressure to equalize ... @17:58:23.10 SS.reconfigure :exhale @17:58:23.15 SS.hold @17:58:34.60 Intake.select :close @17:58:34.75 SSV.dial :exhaust @17:58:35.52 Exhaust.select :open @17:58:35.63 SS.seek 0ml @17:58:45.00 Exhaust.select :close @17:58:45.11 SSV.dial CBV @17:58:45.14
CBV.dial SSV @17:58:45.92 Allowing 130s for 25.0ml of remaining 700.0ml @17:58:45.93 Intake.select :open @17:58:48.09 Intake @14.1psia, vacuum from 8.6 to 11.6psi @17:58:48.11 SS.reconfigure :inhaling 1.43ml/s @17:58:48.15 SS.seek 25ml @17:59:07.30 Stroke completed -- waiting for pressure to equalize ... @17:59:07.31 SS.reconfigure :exhale @17:59:07.35 SS.hold @17:59:20.34 Intake.select :close @17:59:20.49 SSV.dial :exhaust @17:59:21.25 Exhaust.select :open @17:59:21.36 SS.seek 0ml @17:59:30.61 Exhaust.select :close @17:59:30.72 SSV.dial CBV @17:59:30.75
CBV.dial SSV @17:59:31.53 Allowing 130s for 25.0ml of remaining 675.0ml @17:59:31.54 Intake.select :open @17:59:33.70 Intake @14.0psia, vacuum from 8.5 to 11.5psi @17:59:33.72 SS.reconfigure :inhaling 1.43ml/s @17:59:33.77 SS.seek 25ml @17:59:50.88 Vacuum too high -- waiting up to 40 seconds @17:59:50.90 SS.reconfigure :exhale @17:59:50.95 SS.hold @17:59:56.73 Intake @11.8psia, vacuum from 6.3 to 9.3psi @17:59:56.75 SS.reconfigure :inhaling 1.29ml/s @17:59:56.79 SS.seek 25ml @17:59:59.47 Stroke completed -- waiting for pressure to equalize ... @17:59:59.48 SS.reconfigure :exhale @17:59:59.52 SS.hold @18:00:07.14 Intake.select :close @18:00:07.29 SSV.dial :exhaust @18:00:08.05 Exhaust.select :open @18:00:08.16 SS.seek 0ml @18:00:17.28 Exhaust.select :close @18:00:17.39 SSV.dial CBV @18:00:17.42
CBV.dial SSV @18:00:18.20 Allowing 130s for 25.0ml of remaining 650.0ml @18:00:18.21 Intake.select :open @18:00:20.37 Intake @14.0psia, vacuum from 8.5 to 11.5psi @18:00:20.39 SS.reconfigure :inhaling 1.29ml/s @18:00:20.44 SS.seek 25ml @18:00:34.76 Vacuum too high -- waiting up to 40 seconds @18:00:34.77 SS.reconfigure :exhale @18:00:34.82 SS.hold @18:00:39.81 Intake @12.1psia, vacuum from 6.6 to 9.6psi @18:00:39.83 SS.reconfigure :inhaling 1.16ml/s @18:00:39.87 SS.seek 25ml @18:00:46.16 Vacuum too high -- waiting up to 40 seconds @18:00:46.18 SS.reconfigure :exhale @18:00:46.23 SS.hold @18:00:50.72 Intake @12.5psia, vacuum from 7.0 to 10.0psi @18:00:50.74 SS.reconfigure :inhaling 1.04ml/s @18:00:50.78 SS.seek 25ml @18:00:52.79 Stroke completed -- waiting for pressure to equalize ... @18:00:52.80 SS.reconfigure :exhale @18:00:52.85 SS.hold @18:00:56.37 Intake.select :close @18:00:56.52 SSV.dial :exhaust @18:00:57.30 Exhaust.select :open @18:00:57.41 SS.seek 0ml @18:01:06.27 Exhaust.select :close @18:01:06.37 SSV.dial CBV @18:01:06.40
CBV.dial SSV @18:01:07.19 Allowing 130s for 25.0ml of remaining 625.0ml @18:01:07.20 Intake.select :open @18:01:09.36 Intake @14.3psia, vacuum from 8.8 to 11.8psi @18:01:09.38 SS.reconfigure :inhaling 1.04ml/s @18:01:09.42 SS.seek 25ml @18:01:27.66 Vacuum too high -- waiting up to 40 seconds @18:01:27.67 SS.reconfigure :exhale @18:01:27.72 SS.hold @18:01:30.47 Intake @12.1psia, vacuum from 6.6 to 9.6psi @18:01:30.49 SS.reconfigure :inhaling 0.94ml/s @18:01:30.53 SS.seek 25ml @18:01:39.15 Stroke completed -- waiting for pressure to equalize ... @18:01:39.16 SS.reconfigure :exhale @18:01:39.21 SS.hold @18:01:47.78 Intake.select :close @18:01:47.93 SSV.dial :exhaust @18:01:48.70 Exhaust.select :open @18:01:48.81 SS.seek 0ml @18:01:58.08 Exhaust.select :close @18:01:58.18 SSV.dial CBV @18:01:58.21
CBV.dial SSV @18:01:59.00 Allowing 130s for 25.0ml of remaining 600.0ml @18:01:59.01 Intake.select :open @18:02:01.17 Intake @14.6psia, vacuum from 9.1 to 12.1psi @18:02:01.19 SS.reconfigure :inhaling 0.94ml/s @18:02:01.24 SS.seek 25ml @18:02:23.63 Vacuum too high -- waiting up to 40 seconds @18:02:23.64 SS.reconfigure :exhale @18:02:23.69 SS.hold @18:02:27.02 Intake @12.3psia, vacuum from 6.8 to 9.8psi @18:02:27.03 SS.reconfigure :inhaling 0.85ml/s @18:02:27.08 SS.seek 25ml @18:02:33.34 Stroke completed -- waiting for pressure to equalize ... @18:02:33.35 SS.reconfigure :exhale @18:02:33.39 SS.hold @18:02:41.93 Intake.select :close @18:02:42.09 SSV.dial :exhaust @18:02:42.86 Exhaust.select :open @18:02:42.97 SS.seek 0ml @18:02:52.12 Exhaust.select :close @18:02:52.23 SSV.dial CBV @18:02:52.26
CBV.dial SSV @18:02:53.04 Allowing 130s for 25.0ml of remaining 575.0ml @18:02:53.05 Intake.select :open @18:02:55.22 Intake @14.4psia, vacuum from 8.9 to 11.9psi @18:02:55.24 SS.reconfigure :inhaling 0.85ml/s @18:02:55.28 SS.seek 25ml @18:03:21.56 Vacuum too high -- waiting up to 40 seconds @18:03:21.58 SS.reconfigure :exhale @18:03:21.63 SS.hold @18:03:25.68 Intake @12.4psia, vacuum from 6.9 to 9.9psi @18:03:25.69 SS.reconfigure :inhaling 0.77ml/s @18:03:25.74 SS.seek 25ml @18:03:31.42 Stroke completed -- waiting for pressure to equalize ... @18:03:31.43 SS.reconfigure :exhale @18:03:31.47 SS.hold @18:03:38.83 Intake.select :close @18:03:38.98 SSV.dial :exhaust @18:03:39.75 Exhaust.select :open @18:03:39.86 SS.seek 0ml @18:03:48.82 Exhaust.select :close @18:03:48.93 SSV.dial CBV @18:03:48.95
CBV.dial SSV @18:03:49.75 Allowing 130s for 25.0ml of remaining 550.0ml @18:03:49.76 Intake.select :open @18:03:51.92 Intake @14.4psia, vacuum from 8.9 to 11.9psi @18:03:51.94 SS.reconfigure :inhaling 0.77ml/s @18:03:51.98 SS.seek 25ml @18:04:20.13 Vacuum too high -- waiting up to 40 seconds @18:04:20.14 SS.reconfigure :exhale @18:04:20.19 SS.hold @18:04:24.27 Intake @12.7psia, vacuum from 7.2 to 10.2psi @18:04:24.29 SS.reconfigure :inhaling 0.70ml/s @18:04:24.34 SS.seek 25ml @18:04:31.56 Stroke completed -- waiting for pressure to equalize ... @18:04:31.57 SS.reconfigure :exhale @18:04:31.61 SS.hold @18:04:39.06 Intake.select :close @18:04:39.21 SSV.dial :exhaust @18:04:39.98 Exhaust.select :open @18:04:40.09 SS.seek 0ml @18:04:49.48 Exhaust.select :close @18:04:49.59 SSV.dial CBV @18:04:49.61
CBV.dial SSV @18:04:50.40 Allowing 130s for 25.0ml of remaining 525.0ml @18:04:50.41 Intake.select :open @18:04:52.57 Intake @14.6psia, vacuum from 9.1 to 12.1psi @18:04:52.59 SS.reconfigure :inhaling 0.70ml/s @18:04:52.64 SS.seek 25ml @18:05:29.80 Stroke completed -- waiting for pressure to equalize ... @18:05:29.81 SS.reconfigure :exhale @18:05:29.86 SS.hold @18:05:42.50 Intake.select :close @18:05:42.65 SSV.dial :exhaust @18:05:43.42 Exhaust.select :open @18:05:43.53 SS.seek 0ml @18:05:52.84 Exhaust.select :close @18:05:52.94 SSV.dial CBV @18:05:52.97
CBV.dial SSV @18:05:53.76 Allowing 130s for 25.0ml of remaining 500.0ml @18:05:53.77 Intake.select :open @18:05:55.93 Intake @14.5psia, vacuum from 9.0 to 12.0psi @18:05:55.95 SS.reconfigure :inhaling 0.70ml/s @18:05:56.00 SS.seek 25ml @18:06:21.12 Vacuum too high -- waiting up to 40 seconds @18:06:21.14 SS.reconfigure :exhale @18:06:21.19 SS.hold @18:06:26.28 Intake @13.2psia, vacuum from 7.7 to 10.7psi @18:06:26.30 SS.reconfigure :inhaling 0.62ml/s @18:06:26.35 SS.seek 25ml @18:06:39.97 Stroke completed -- waiting for pressure to equalize ... @18:06:39.98 SS.reconfigure :exhale @18:06:40.03 SS.hold @18:06:48.58 Intake.select :close @18:06:48.73 SSV.dial :exhaust @18:06:49.49 Exhaust.select :open @18:06:49.60 SS.seek 0ml @18:06:58.91 Exhaust.select :close @18:06:59.02 SSV.dial CBV @18:06:59.05
CBV.dial SSV @18:06:59.84 Allowing 130s for 25.0ml of remaining 475.0ml @18:06:59.85 Intake.select :open @18:07:02.01 Intake @14.9psia, vacuum from 9.4 to 12.4psi @18:07:02.03 SS.reconfigure :inhaling 0.62ml/s @18:07:02.07 SS.seek 25ml @18:07:31.12 Vacuum too high -- waiting up to 40 seconds @18:07:31.13 SS.reconfigure :exhale @18:07:31.18 SS.hold @18:07:35.06 Intake @13.3psia, vacuum from 7.8 to 10.8psi @18:07:35.07 SS.reconfigure :inhaling 0.56ml/s @18:07:35.12 SS.seek 25ml @18:07:49.32 Stroke completed -- waiting for pressure to equalize ... @18:07:49.33 SS.reconfigure :exhale @18:07:49.37 SS.hold @18:07:59.31 Intake.select :close @18:07:59.46 SSV.dial :exhaust @18:08:00.23 Exhaust.select :open @18:08:00.34 SS.seek 0ml @18:08:09.57 Exhaust.select :close @18:08:09.68 SSV.dial CBV @18:08:09.70
CBV.dial SSV @18:08:10.49 Allowing 130s for 25.0ml of remaining 450.0ml @18:08:10.50 Intake.select :open @18:08:12.67 Intake @14.8psia, vacuum from 9.3 to 12.3psi @18:08:12.68 SS.reconfigure :inhaling 0.56ml/s @18:08:12.73 SS.seek 25ml @18:08:50.15 Vacuum too high -- waiting up to 40 seconds @18:08:50.17 SS.reconfigure :exhale @18:08:50.22 SS.hold @18:08:56.48 Intake @13.5psia, vacuum from 8.0 to 11.0psi @18:08:56.50 SS.reconfigure :inhaling 0.51ml/s @18:08:56.54 SS.seek 25ml @18:09:05.77 Stroke completed -- waiting for pressure to equalize ... @18:09:05.78 SS.reconfigure :exhale @18:09:05.82 SS.hold @18:09:15.59 Intake.select :close @18:09:15.74 SSV.dial :exhaust @18:09:16.51 Exhaust.select :open @18:09:16.63 SS.seek 0ml @18:09:25.88 Exhaust.select :close @18:09:25.99 SSV.dial CBV @18:09:26.01
CBV.dial SSV @18:09:26.82 Allowing 130s for 25.0ml of remaining 425.0ml @18:09:26.83 Intake.select :open @18:09:28.99 Intake @14.8psia, vacuum from 9.3 to 12.3psi @18:09:29.01 SS.reconfigure :inhaling 0.51ml/s @18:09:29.06 SS.seek 25ml @18:10:11.28 Vacuum too high -- waiting up to 40 seconds @18:10:11.29 SS.reconfigure :exhale @18:10:11.34 SS.hold @18:10:17.92 Intake @13.8psia, vacuum from 8.3 to 11.3psi @18:10:17.93 SS.reconfigure :inhaling 0.46ml/s @18:10:17.98 SS.seek 25ml @18:10:27.49 Stroke completed -- waiting for pressure to equalize ... @18:10:27.50 SS.reconfigure :exhale @18:10:27.54 SS.hold @18:10:37.31 Intake.select :close @18:10:37.46 SSV.dial :exhaust @18:10:38.23 Exhaust.select :open @18:10:38.34 SS.seek 0ml @18:10:47.27 Exhaust.select :close @18:10:47.38 SSV.dial CBV @18:10:47.40
CBV.dial SSV @18:10:48.21 Allowing 130s for 25.0ml of remaining 400.0ml @18:10:48.22 Intake.select :open @18:10:50.38 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:10:50.40 SS.reconfigure :inhaling 0.46ml/s @18:10:50.45 SS.seek 25ml @18:11:40.21 Vacuum too high -- waiting up to 40 seconds @18:11:40.22 SS.reconfigure :exhale @18:11:40.28 SS.hold @18:11:46.00 Intake @13.7psia, vacuum from 8.2 to 11.2psi @18:11:46.02 SS.reconfigure :inhaling 0.42ml/s @18:11:46.06 SS.seek 25ml @18:11:52.45 Stroke completed -- waiting for pressure to equalize ... @18:11:52.46 SS.reconfigure :exhale @18:11:52.94 SS.hold @18:12:02.73 Intake.select :close @18:12:02.88 SSV.dial :exhaust @18:12:03.64 Exhaust.select :open @18:12:03.75 SS.seek 0ml @18:12:12.94 Exhaust.select :close @18:12:13.05 SSV.dial CBV @18:12:13.07
CBV.dial SSV @18:12:13.86 Allowing 130s for 25.0ml of remaining 375.0ml @18:12:13.87 Intake.select :open @18:12:16.03 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:12:16.05 SS.reconfigure :inhaling 0.42ml/s @18:12:16.10 SS.seek 25ml @18:13:17.90 Stroke completed -- waiting for pressure to equalize ... @18:13:17.91 SS.reconfigure :exhale @18:13:17.96 SS.hold @18:13:34.66 Intake.select :close @18:13:34.81 SSV.dial :exhaust @18:13:35.58 Exhaust.select :open @18:13:35.69 SS.seek 0ml @18:13:45.14 Exhaust.select :close @18:13:45.25 SSV.dial CBV @18:13:45.27
CBV.dial SSV @18:13:46.08 Allowing 130s for 25.0ml of remaining 350.0ml @18:13:46.09 Intake.select :open @18:13:48.25 Intake @15.1psia, vacuum from 9.6 to 12.6psi @18:13:48.27 SS.reconfigure :inhaling 0.42ml/s @18:13:48.32 SS.seek 25ml @18:14:49.71 Stroke completed -- waiting for pressure to equalize ... @18:14:49.72 SS.reconfigure :exhale @18:14:49.77 SS.hold @18:15:10.14 Intake.select :close @18:15:10.29 SSV.dial :exhaust @18:15:11.07 Exhaust.select :open @18:15:11.18 SS.seek 0ml @18:15:20.51 Exhaust.select :close @18:15:20.62 SSV.dial CBV @18:15:20.65
CBV.dial SSV @18:15:21.44 Allowing 130s for 25.0ml of remaining 325.0ml @18:15:21.45 Intake.select :open @18:15:23.61 Intake @14.9psia, vacuum from 9.4 to 12.4psi @18:15:23.63 SS.reconfigure :inhaling 0.42ml/s @18:15:23.67 SS.seek 25ml @18:16:02.96 Vacuum too high -- waiting up to 40 seconds @18:16:02.97 SS.reconfigure :exhale @18:16:03.02 SS.hold @18:16:11.01 Intake @15.0psia, vacuum from 9.5 to 12.5psi @18:16:11.03 SS.reconfigure :inhaling 0.38ml/s @18:16:11.07 SS.seek 25ml @18:16:36.09 Stroke completed -- waiting for pressure to equalize ... @18:16:36.10 SS.reconfigure :exhale @18:16:36.14 SS.hold @18:16:52.35 Intake.select :close @18:16:52.50 SSV.dial :exhaust @18:16:53.26 Exhaust.select :open @18:16:53.37 SS.seek 0ml @18:17:02.81 Exhaust.select :close @18:17:02.92 SSV.dial CBV @18:17:02.94
CBV.dial SSV @18:17:03.73 Allowing 130s for 25.0ml of remaining 300.0ml @18:17:03.74 Intake.select :open @18:17:05.90 Intake @15.4psia, vacuum from 9.9 to 12.9psi @18:17:05.92 SS.reconfigure :inhaling 0.38ml/s @18:17:05.97 SS.seek 25ml @18:18:03.30 Vacuum too high -- waiting up to 40 seconds @18:18:03.31 SS.reconfigure :exhale @18:18:03.36 SS.hold @18:18:11.23 Intake @14.3psia, vacuum from 8.8 to 11.8psi @18:18:11.25 SS.reconfigure :inhaling 0.33ml/s @18:18:11.30 SS.seek 25ml @18:18:23.85 Stroke completed -- waiting for pressure to equalize ... @18:18:23.86 SS.reconfigure :exhale @18:18:23.91 SS.hold @18:18:38.27 Intake.select :close @18:18:38.43 SSV.dial :exhaust @18:18:39.20 Exhaust.select :open @18:18:39.31 SS.seek 0ml @18:18:48.34 Exhaust.select :close @18:18:48.45 SSV.dial CBV @18:18:48.47
CBV.dial SSV @18:18:49.27 Allowing 130s for 25.0ml of remaining 275.0ml @18:18:49.28 Intake.select :open @18:18:51.44 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:18:51.46 SS.reconfigure :inhaling 0.33ml/s @18:18:51.51 SS.seek 25ml @18:20:09.75 Stroke completed -- waiting for pressure to equalize ... @18:20:09.76 SS.reconfigure :exhale @18:20:09.81 SS.hold @18:20:32.66 Intake.select :close @18:20:32.81 SSV.dial :exhaust @18:20:33.59 Exhaust.select :open @18:20:33.70 SS.seek 0ml @18:20:43.18 Exhaust.select :close @18:20:43.29 SSV.dial CBV @18:20:43.31
CBV.dial SSV @18:20:44.12 Allowing 130s for 25.0ml of remaining 250.0ml @18:20:44.13 Intake.select :open @18:20:46.29 Intake @15.5psia, vacuum from 10.0 to 13.0psi @18:20:46.31 SS.reconfigure :inhaling 0.33ml/s @18:20:46.36 SS.seek 25ml @18:21:48.29 Vacuum too high -- waiting up to 40 seconds @18:21:48.31 SS.reconfigure :exhale @18:21:48.36 SS.hold @18:21:57.90 Intake @14.8psia, vacuum from 9.3 to 12.3psi @18:21:57.92 SS.reconfigure :inhaling 0.30ml/s @18:21:57.97 SS.seek 25ml @18:22:16.58 Stroke completed -- waiting for pressure to equalize ... @18:22:16.59 SS.reconfigure :exhale @18:22:16.63 SS.hold @18:22:33.41 Intake.select :close @18:22:33.56 SSV.dial :exhaust @18:22:34.32 Exhaust.select :open @18:22:34.43 SS.seek 0ml @18:22:43.80 Exhaust.select :close @18:22:43.91 SSV.dial CBV @18:22:43.94
CBV.dial SSV @18:22:44.72 Allowing 130s for 25.0ml of remaining 225.0ml @18:22:44.73 Intake.select :open @18:22:46.89 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:22:46.91 SS.reconfigure :inhaling 0.30ml/s @18:22:46.96 SS.seek 25ml @18:23:58.79 Vacuum too high -- waiting up to 40 seconds @18:23:58.80 SS.reconfigure :exhale @18:23:58.85 SS.hold @18:24:07.50 Intake @14.9psia, vacuum from 9.4 to 12.4psi @18:24:07.51 SS.reconfigure :inhaling 0.28ml/s @18:24:07.56 SS.seek 25ml @18:24:24.39 Stroke completed -- waiting for pressure to equalize ... @18:24:24.40 SS.reconfigure :exhale @18:24:24.45 SS.hold @18:24:42.79 Intake.select :close @18:24:42.94 SSV.dial :exhaust @18:24:43.70 Exhaust.select :open @18:24:43.81 SS.seek 0ml @18:24:53.38 Exhaust.select :close @18:24:53.85 SSV.dial CBV @18:24:53.88
CBV.dial SSV @18:24:54.67 Allowing 130s for 25.0ml of remaining 200.0ml @18:24:54.68 Intake.select :open @18:24:56.84 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:24:56.86 SS.reconfigure :inhaling 0.28ml/s @18:24:56.91 SS.seek 25ml @18:26:33.62 Stroke completed -- waiting for pressure to equalize ... @18:26:33.63 SS.reconfigure :exhale @18:26:33.68 SS.hold @18:27:00.22 Intake.select :close @18:27:00.38 SSV.dial :exhaust @18:27:01.15 Exhaust.select :open @18:27:01.26 SS.seek 0ml @18:27:10.77 Exhaust.select :close @18:27:10.88 SSV.dial CBV @18:27:10.92
CBV.dial SSV @18:27:11.69 Allowing 130s for 25.0ml of remaining 175.0ml @18:27:11.70 Intake.select :open @18:27:13.86 Intake @15.9psia, vacuum from 10.0 to 13.0psi @18:27:13.88 SS.reconfigure :inhaling 0.28ml/s @18:27:13.93 SS.seek 25ml @18:28:46.66 Vacuum too high -- waiting up to 40 seconds @18:28:46.67 SS.reconfigure :exhale @18:28:46.73 SS.hold @18:28:57.96 Intake @15.8psia, vacuum from 10.0 to 13.0psi @18:28:57.98 SS.reconfigure :inhaling 0.25ml/s @18:28:58.03 SS.seek 25ml @18:29:04.63 Stroke completed -- waiting for pressure to equalize ... @18:29:04.64 SS.reconfigure :exhale @18:29:04.69 SS.hold @18:29:19.15 Intake.select :close @18:29:19.30 SSV.dial :exhaust @18:29:20.06 Exhaust.select :open @18:29:20.17 SS.seek 0ml @18:29:29.73 Exhaust.select :close @18:29:29.84 SSV.dial CBV @18:29:29.86
CBV.dial SSV @18:29:30.67 Allowing 130s for 25.0ml of remaining 150.0ml @18:29:30.68 Intake.select :open @18:29:32.84 Intake @16.1psia, vacuum from 10.0 to 13.0psi @18:29:32.86 SS.reconfigure :inhaling 0.25ml/s @18:29:32.91 SS.seek 25ml @18:31:23.85 Stroke completed -- waiting for pressure to equalize ... @18:31:23.86 SS.reconfigure :exhale @18:31:23.91 SS.hold @18:31:40.69 SS.reconfigure :default @18:31:40.73 SS.hold @18:31:40.79 Intake.select :close @18:31:40.94 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 875.0ml @18:31:40.96 SSV.dial :exhaust @18:31:41.71 Exhaust.select :open @18:31:41.82 SS.reconfigure :exhale @18:31:41.87 SS.seek 0ml @18:31:51.57 Exhaust.select :close @18:31:51.68 Sampled 875.0ml @18:31:51.69 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @18:31:51.70 Intake.select :close @18:31:51.77 CSV.dial :waste @18:31:51.93 SSV.dial CBV,avoiding: :exhaust @18:31:51.95 CS.seek 0ml @18:31:51.98
CBV.dial SSV @18:31:52.11 CSV.dial :air @18:31:52.87 CS.seek 10ml @18:31:53.92
CTV.dial :air @18:31:54.70 delay 15 seconds @18:32:09.72 SSV.dial :spit,avoiding: :exhaust @18:32:11.08 CTV.dial CSV,avoiding: :intake @18:32:11.11
SS.seek 0ml @18:32:11.23 SSV.dial CBV,avoiding: :exhaust @18:32:17.11 SSV.dial between CBV and plug @18:32:17.61 CBV.dial between SSV and waste2 @18:32:17.99 CSV.dial between air and CTV @18:32:18.42 CS.seek 8.085ml @18:32:23.68 CTV.dial CSV,avoiding: :air @18:32:23.70
CSV.dial CTV @18:32:24.07 CS.reconfigure :pressurize @18:32:24.11 CS.seek 0ml @18:32:39.27 BubblePoint: Intake @24.1psia, Outlet @0.9psia @18:32:39.28 CBV.dial SSV @18:32:39.63 SS.reconfigure :default @18:32:40.10 SS.seek 10ml @18:32:45.74 delay 15 seconds @18:33:00.87 Evacuated: Intake @23.2psia, Outlet @22.0psia @18:33:00.89 SSV.dial :spit,avoiding: :exhaust @18:33:01.95 SS.reconfigure :exhale @18:33:02.00 SS.seek 0ml @18:33:07.14 SS.reconfigure :fast @18:33:07.19 SS.seek 5ml @18:33:09.93 SSV.dial CBV,avoiding: :exhaust @18:33:09.96
CBV.dial SSV @18:33:11.34 CTV.dial :air,avoiding: :intake @18:33:12.72 CSV.dial CTV @18:33:12.77
SS.seek 10ml @18:33:13.94 CSV.dial :air @18:33:14.38 CS.reconfigure :fast @18:33:14.43 CS.seek 0ml @18:33:15.26
SSV.dial :spit,avoiding: :exhaust @18:33:16.62 SS.reconfigure :exhale @18:33:16.67 SS.seek 0ml @18:33:20.70 CS.stop @18:33:21.63
SS.reconfigure :fast @18:33:21.67 SS.seek 5ml @18:33:24.42 SSV.dial CBV,avoiding: :exhaust @18:33:24.44
CBV.dial SSV @18:33:26.86 SSV.dial :spit,avoiding: :exhaust @18:33:28.23 SS.reconfigure :default @18:33:28.28 SS.seek 0ml @18:33:31.48 SS.stop @18:33:31.52 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @18:33:31.53 Intake.select :close @18:33:31.60 CSV.dial :waste @18:33:31.73 SSV.dial CBV,avoiding: :exhaust @18:33:31.75
CBV.dial SSV @18:33:32.36 CS.seek 0ml @18:33:32.43 CSV.dial :air @18:33:33.13
CTV.dial :air @18:33:33.21 CS.seek 10ml @18:33:33.27
delay 15 seconds @18:33:48.29 SSV.dial :spit,avoiding: :exhaust @18:33:49.65 CTV.dial CSV,avoiding: :intake @18:33:49.69
SS.reconfigure :exhale @18:33:49.78 SS.seek 0ml @18:33:50.32 SSV.dial CBV,avoiding: :exhaust @18:33:57.30 SSV.dial between CBV and plug @18:33:57.79 CBV.dial between SSV and waste2 @18:33:58.18 CSV.dial between air and CTV @18:33:58.68 CS.seek 7.34ml @18:34:05.64 CTV.dial CSV,avoiding: :air @18:34:05.67
CSV.dial CTV @18:34:05.97 CS.reconfigure :pressurize @18:34:06.02 CS.seek 0ml @18:34:20.33 BubblePoint: Intake @24.0psia, Outlet @2.7psia @18:34:20.34 CBV.dial SSV @18:34:20.69 SS.reconfigure :default @18:34:20.74 SS.seek 10ml @18:34:26.29 delay 15 seconds @18:34:41.41 Evacuated: Intake @24.0psia, Outlet @22.7psia @18:34:41.43 SSV.dial :spit,avoiding: :exhaust @18:34:42.50 SS.reconfigure :exhale @18:34:42.54 SS.seek 0ml @18:34:47.59 SS.reconfigure :fast @18:34:47.64 SS.seek 5ml @18:34:50.43 SSV.dial CBV,avoiding: :exhaust @18:34:50.46
CBV.dial SSV @18:34:51.84 CTV.dial :air,avoiding: :intake @18:34:53.22 CSV.dial CTV @18:34:53.26
SS.seek 10ml @18:34:54.38 CSV.dial :air @18:34:54.83 CS.reconfigure :fast @18:34:54.88 CS.seek 0ml @18:34:55.76
SSV.dial :spit,avoiding: :exhaust @18:34:57.12 SS.reconfigure :exhale @18:34:57.17 SS.seek 0ml @18:35:00.23 CS.stop @18:35:02.08
SS.reconfigure :fast @18:35:02.12 SS.seek 5ml @18:35:04.90 SSV.dial CBV,avoiding: :exhaust @18:35:04.93
CBV.dial SSV @18:35:07.31 SSV.dial :spit,avoiding: :exhaust @18:35:08.67 SS.reconfigure :default @18:35:08.71 SS.seek 0ml @18:35:11.93 SS.stop @18:35:11.97 Lysing: Adding 2.0ml AqMeOH @18:35:11.98 CBV.dial between CSV and waste1,avoiding: :DBV @18:35:13.50 CTV.dial CSV @18:35:14.86 CSV.dial CSR @18:35:15.64 Pulling 1ml of CSR.AqMeOH @18:35:15.65 CSR.select :AqMeOH @18:35:15.77 CS.reconfigure :default @18:35:15.81 CS.seek 1ml @18:35:19.42 delay 5 seconds @18:35:24.44 CSR.select :air @18:35:24.60 CS.seek 6ml @18:35:38.70 delay 2 seconds @18:35:40.72 CSV.dial :waste2 @18:35:41.28 CS.reconfigure :fast @18:35:41.33 CS.seek 0ml @18:35:55.82 CSV.dial CSR @18:35:56.26 Pulling 2ml of CSR.AqMeOH @18:35:56.27 CSR.select :AqMeOH @18:35:56.39 CS.reconfigure :default @18:35:56.43 CS.seek 2ml @18:36:02.36 delay 5 seconds @18:36:07.37 CSR.select :air @18:36:07.54 CS.seek 5ml @18:36:16.07 delay 2 seconds @18:36:18.09 CSV.dial CTV @18:36:18.55 CS.seek 1ml @18:36:29.71 Before lysing Collection Puck Heater at 14.9C/20.2psia @18:36:29.74 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @18:36:29.76 CH.seek 70C,8:00 @18:38:58.05 CH.hold 10:00,70C @18:38:58.23 Begun lysing Collection Puck Heater at 68C/26.3psia @18:48:58.26 Ended lysing Collection Puck Heater at 70C/23.1psia @18:48:58.28 CS.seek 0ml @18:49:01.85 CTV.dial between spare and air,avoiding: :intake @18:49:02.91 CSV.dial :air @18:49:03.36 CS.seek 5ml @18:49:17.03 CSV.dial CTV @18:49:17.49 delay 2 seconds @18:49:19.50 CSV.dial :air @18:49:19.95 delay 2 seconds @18:49:21.97 CSV.dial CTV @18:49:22.50 delay 2 seconds @18:49:24.52 CSV.dial :air @18:49:24.97 delay 2 seconds @18:49:27.00 CS.seek 0ml @18:49:40.62 Puck @33.4psia after final lysis pressurization @18:49:40.63 Recovering Lysate into Collection syringe @18:49:40.64 CSV.dial CBV @18:49:41.64 CBV.dial CSV @18:49:42.03 CS.seek 5ml @18:49:55.69 delay 5 seconds @18:50:00.71 CTV.dial :air @18:50:01.24 CS.seek 7ml @18:50:07.16 delay 5 seconds @18:50:12.18 CSV.dial :air2 @18:50:12.62 CS.seek 10ml @18:50:21.19 CSV.dial between air and CTV,avoiding: CBV @18:50:22.33 Lysing Complete @18:50:22.40 Storing sh1 puck in Collection Clamp to tube 3 @18:50:22.57 SC.reconfigure :tight @18:50:22.62 SC.seek 3 @18:50:26.30 SC.configure :limp @18:50:26.44 SE.reconfigure :place @18:50:26.53
Elbow.reconfigure :tight @18:50:26.57 SE.seek :beamBreak @18:50:26.60
Elbow.seek CC @18:50:26.77 Elbow.reconfigure :loose @18:50:26.87 Forearm.seek CC @18:50:27.90 Hand.close @18:50:28.65 CC.seek :open @18:50:29.44 SE.configure :default @18:50:29.49 SE.seek :place @18:50:32.85
Forearm.seek :retract @18:50:33.90 Elbow.reconfigure :tight @18:50:33.95 Elbow.seek 3 @18:50:35.63 Elbow.reconfigure :limp @18:50:35.68 Forearm.seek 3 @18:50:37.26 Elbow.hold @18:50:37.33 Elbow.reconfigure :tight @18:50:37.38 Hand.open @18:50:38.26 Forearm.force -20 @18:50:38.31 Forearm.seek 3 + 318 counts @18:50:38.60 Forearm.reconfigure :tight @18:50:38.64 Forearm.seek 3 + 800 counts @18:50:38.91 SE.seek :down @18:50:40.04
Forearm.reconfigure :loose @18:50:40.09 Forearm.seek :retract @18:50:41.62 SE.stop @18:50:41.66
Tranferring DA @18:50:41.67 Beginning samplePassC @18:50:41.69 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:50:41.87 Elbow.seek FlushPuck::Garage @18:50:43.68 Elbow.reconfigure :loose @18:50:43.77 Forearm.seek :garage @18:50:44.82 Hand.close @18:50:45.64 Forearm.seek :retract @18:50:46.62 Elbow.reconfigure :tight @18:50:46.66 Elbow.seek CC @18:50:49.51 Elbow.reconfigure :loose @18:50:49.61 Forearm.reconfigure :tight @18:50:49.65 Forearm.seek CC @18:50:50.96 CC.seek :closed @18:50:54.97 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.94 @18:50:55.04 Hand.open @18:50:55.83 Forearm.reconfigure :loose @18:50:55.88 Forearm.seek :retract @18:50:56.86 PSV.dial PSR @18:50:58.25 Pulling 1.5ml of PSR.flush @18:50:58.26 PSR.select :flush @18:50:58.38 PS.seek 1.5ml @18:51:14.49 delay 5 seconds @18:51:19.51 PSV.dial :waste1 @18:51:20.07 PS.seek 0ml @18:51:36.24 PSV.dial PSR @18:51:36.69 PSR.select :air @18:51:36.81 PS.seek 2.5ml @18:52:03.09 PRV.dial CBV,avoiding: :ab2 @18:52:03.11
CBV.dial PRV @18:52:04.87 PSV.dial :mixloop @18:52:05.90 CTV.dial :air,avoiding: :intake @18:52:05.97 PTV.dial :PRVmixing @18:52:07.26 PS.seek 0ml @18:52:33.55 PSV.dial :air @18:52:34.01 PS.seek 2.5ml @18:53:00.43 PSV.dial :mixloop @18:53:00.90 PS.seek 0ml @18:53:27.27 PSV.dial :air @18:53:27.74 PS.seek 2.5ml @18:53:54.36 PSV.dial :mixloop @18:53:54.84 PS.seek 0ml @18:54:21.15 Garaging FlushPuck in Collection Clamp @18:54:21.22 Elbow.reconfigure :tight @18:54:21.26 Elbow.seek CC @18:54:21.38 Elbow.reconfigure :loose @18:54:21.48 Forearm.seek CC @18:54:22.52 Hand.close @18:54:23.27 CC.seek :open @18:54:27.74 Forearm.seek :retract @18:54:28.93 Elbow.reconfigure :tight @18:54:28.97 Elbow.seek :garage @18:54:31.82 Elbow.reconfigure :loose @18:54:31.92 Forearm.reconfigure :tight @18:54:31.97 Forearm.seek :garage @18:54:33.09 Hand.open @18:54:33.83 Forearm.reconfigure :loose @18:54:33.87 Forearm.seek :retract @18:54:34.94 PSV.dial :mixloop @18:54:35.11 PTV.dial :PRVmixing @18:54:35.19 PRV.dial CBV @18:54:35.22
CBV.dial PRV @18:54:35.43 CTV.dial :air,avoiding: :intake @18:54:35.50 Loading lyfil puck from tube 4 into Collection Clamp @18:54:35.73 SC.reconfigure :tight @18:54:35.78 SC.seek 4 @18:54:39.37 SC.configure :limp @18:54:39.58 Elbow.reconfigure :tight @18:54:39.63 Elbow.seek 4 @18:54:41.51 Forearm.reconfigure :tight @18:54:41.56 Forearm.seek 4 + 800 counts @18:54:42.62 SE.reconfigure :pick @18:54:42.68 SE.seek :beamBreak @18:54:43.80 SE.configure :default @18:54:43.95 Elbow.seek 4 @18:54:44.14 Forearm.seek 4 + 300 counts @18:54:44.31 Forearm.reconfigure :loose @18:54:44.36 Hand.close @18:54:45.60 SE.seek :down @18:54:45.80
Forearm.seek :retract @18:54:46.46 SE.stop @18:54:46.75
Elbow.seek CC @18:54:48.35 Elbow.reconfigure :loose @18:54:48.45 Forearm.reconfigure :tight @18:54:48.50 Forearm.seek CC @18:54:49.84 CC.seek :closed @18:54:53.81 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 2.06 @18:54:53.83 Hand.open @18:54:54.58 Forearm.reconfigure :loose @18:54:54.63 Forearm.seek :retract @18:54:55.61 CTV.dial CSV,avoiding: :intake @18:54:56.97 CSV.dial CTV @18:54:57.29 PS.seek 2.5ml @18:55:23.80 CS.seek 5ml @18:55:37.43 delay 8 seconds @18:55:45.46 PSV.dial :air @18:55:45.91 delay 2 seconds @18:55:47.93 PSV.dial :waste2 @18:55:48.67 PS.seek 0ml @18:56:14.99 CBV.dial between PRV and waste1 @18:56:15.31 CSV.dial :air @18:56:15.77 CS.seek 10ml @18:56:29.40 CSV.dial CTV @18:56:29.86 CS.seek 0ml @18:56:56.28 CBV.dial :waste1 @18:56:56.61 delay 5 seconds @18:57:01.63 CTV.dial :air,avoiding: :intake @18:57:03.50 Storing lyfil puck in Collection Clamp to tube 3 @18:57:03.67 SC.reconfigure :tight @18:57:03.72 SC.seek 3 @18:57:07.37 SC.configure :limp @18:57:07.58 Elbow.reconfigure :tight @18:57:07.63 Elbow.seek 3 @18:57:09.32 Forearm.reconfigure :tight @18:57:09.37 Forearm.seek 3 + 800 counts @18:57:10.39 SE.reconfigure :pick @18:57:10.44 SE.seek :beamBreak @18:57:13.31 SE.configure :default @18:57:13.47 Elbow.reconfigure :limp @18:57:13.52 Forearm.reconfigure :loose @18:57:13.56 Forearm.seek 3 @18:57:13.95 Elbow.hold @18:57:14.02 Elbow.reconfigure :tight @18:57:14.11 SE.seek 0.11 Pucks below Top Plate @18:57:14.55 Forearm.force -20 @18:57:14.59 Forearm.seek 3 + 319 counts @18:57:14.88 Forearm.reconfigure :tight @18:57:14.93 Forearm.seek 3 + 800 counts @18:57:15.21 SE.seek 1.11 Pucks below Top Plate @18:57:15.72 Forearm.reconfigure :loose @18:57:15.77 Forearm.seek :retract @18:57:16.67 Elbow.seek CC @18:57:18.30 Elbow.reconfigure :loose @18:57:18.40 Forearm.seek CC @18:57:19.34 Hand.close @18:57:20.08 CC.seek :open @18:57:24.29 Forearm.seek :retract @18:57:25.28 Elbow.reconfigure :tight @18:57:25.33 Elbow.seek 3 @18:57:27.05 Elbow.reconfigure :limp @18:57:27.10 Forearm.seek 3 @18:57:28.22 Elbow.hold @18:57:28.29 Elbow.reconfigure :tight @18:57:28.33 Hand.open @18:57:29.14 Forearm.force -20 @18:57:29.18 Forearm.seek 3 + 319 counts @18:57:29.46 Forearm.reconfigure :tight @18:57:29.51 Forearm.seek 3 + 800 counts @18:57:29.97 SE.seek :down @18:57:31.11
Forearm.reconfigure :loose @18:57:31.15 Forearm.seek :retract @18:57:32.58 SE.stop @18:57:32.63
PSV.dial :air @18:57:33.37 PS.seek 2.5ml @18:57:59.74 delay 1 second @18:58:00.75 PSV.dial :mixloop @18:58:01.20 delay 1 second @18:58:02.22 PSV.dial :air @18:58:02.69 delay 1 second @18:58:03.70 PSV.dial :mixloop @18:58:04.17 delay 1 second @18:58:05.19 PSV.dial :air @18:58:05.66 delay 1 second @18:58:06.68 PS.seek 0ml @18:58:33.08 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:58:33.26 Elbow.seek FlushPuck::Garage @18:58:35.05 Elbow.reconfigure :loose @18:58:35.15 Forearm.seek :garage @18:58:36.17 Hand.close @18:58:37.01 Forearm.seek :retract @18:58:38.02 Elbow.reconfigure :tight @18:58:38.06 Elbow.seek PC @18:58:39.42 Elbow.reconfigure :loose @18:58:39.51 Forearm.reconfigure :tight @18:58:39.56 Forearm.seek PC @18:58:40.94 PC.seek :closed @18:58:45.37 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.52 @18:58:45.43 Hand.open @18:58:46.20 Forearm.reconfigure :loose @18:58:46.24 Forearm.seek :retract @18:58:47.25 PRV.dial :air @18:58:47.72 PSV.dial :mixloop @18:58:48.26 PS.seek 0.3ml @18:58:52.30 PSV.dial PSR @18:58:53.35 Pulling 0.4ml of PSR.air @18:58:53.36 PSR.select :air @18:58:53.38 PS.seek 0.7ml @18:58:58.33 delay 3 seconds @18:59:01.35 PSR.select :air @18:59:01.37 PS.seek 2.5ml @18:59:20.61 delay 3 seconds @18:59:23.63 PSV.dial :mixloop @18:59:24.67 PTV.dial :mixing @18:59:25.42 PBV.dial :waste2 @18:59:26.43 PS.seek 0ml @18:59:52.80 Garaging FlushPuck in Processing Clamp @18:59:53.32 Elbow.reconfigure :tight @18:59:53.37 Elbow.seek PC @18:59:53.55 Elbow.reconfigure :loose @18:59:53.66 Forearm.seek PC @18:59:54.75 Hand.close @18:59:55.52 PC.seek :open @19:00:00.01 Forearm.seek :retract @19:00:01.08 Elbow.reconfigure :tight @19:00:01.12 Elbow.seek :garage @19:00:02.49 Elbow.reconfigure :loose @19:00:02.59 Forearm.reconfigure :tight @19:00:02.63 Forearm.seek :garage @19:00:03.73 Hand.open @19:00:04.57 Forearm.reconfigure :loose @19:00:04.61 Forearm.seek :retract @19:00:05.62 Loading da puck from tube 4 into Processing Clamp @19:00:05.85 SC.reconfigure :tight @19:00:05.90 SC.seek 4 @19:00:09.55 SC.configure :limp @19:00:09.76 Elbow.reconfigure :tight @19:00:09.81 Elbow.seek 4 @19:00:11.64 Forearm.reconfigure :tight @19:00:11.69 Forearm.seek 4 + 800 counts @19:00:12.81 SE.reconfigure :pick @19:00:12.86 SE.seek :beamBreak @19:00:14.06 SE.configure :default @19:00:14.21 Elbow.seek 4 @19:00:14.39 Forearm.seek 4 + 300 counts @19:00:14.67 Forearm.reconfigure :loose @19:00:15.16 Hand.close @19:00:15.90 SE.seek :down @19:00:16.10
Forearm.seek :retract @19:00:16.87 SE.stop @19:00:17.20
Elbow.seek PC @19:00:19.71 Elbow.reconfigure :loose @19:00:19.81 Forearm.reconfigure :tight @19:00:19.85 Forearm.seek PC @19:00:21.20 PC.seek :closed @19:00:25.63 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.48 @19:00:25.64 Hand.open @19:00:26.45 Forearm.reconfigure :loose @19:00:26.49 Forearm.seek :retract @19:00:27.53 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @19:00:27.55 PH.seek 28C,5:00 @19:01:10.19 PH.hold 2:00:00,28C @19:01:10.25 delay 1:30 @19:02:40.28 PSR.select :air @19:02:40.30 PSV.dial PSR @19:02:41.34 Pulling 0.5ml of PSR.wash @19:02:41.35 PSR.select :wash @19:02:41.47 PS.seek 0.5ml @19:02:47.39 delay 5 seconds @19:02:52.41 PSR.select :air @19:02:52.53 PS.seek 2ml @19:03:08.64 PTV.dial :puck @19:03:09.37 PSV.dial PTV @19:03:10.12 PBV.dial :waste1 @19:03:11.56 PS.seek 1.5ml @19:03:17.45 delay 3 seconds @19:03:20.47 PBV.dial between PSV and waste1 @19:03:20.85 PS.seek 0ml @19:03:36.98 delay 5:00 @19:08:37.00 recoverPSRreagent08 @19:08:37.01 PTV.dial :puck @19:08:37.09 PBV.dial :waste1 @19:08:37.48 delay 30 seconds @19:09:07.50 PBV.dial between PSV and waste1 @19:09:07.90 PSR.select :air @19:09:07.92 PSV.dial PSR @19:09:08.67 PS.reconfigure :fast @19:09:08.72 PS.seek 2.5ml @19:09:32.07 PSV.dial PTV @19:09:32.82 delay 5 seconds @19:09:37.85 PSV.dial PSR @19:09:38.59 delay 5 seconds @19:09:43.61 PSV.dial PTV @19:09:44.35 delay 5 seconds @19:09:49.37 PSV.dial :air @19:09:50.14 PS.seek 0ml @19:10:13.26 PTV.dial :PRVmixing,via: PRV @19:10:15.01 PSV.dial :mixloop @19:10:15.48 PRV.dial :ab1 @19:10:16.22 PS.reconfigure :slow @19:10:16.26 PS.seek 0.15ml @19:10:19.79 delay 2 seconds @19:10:21.81 CBV.dial PRV @19:10:21.83
PRV.dial CBV @19:10:22.41 PS.seek 0.34ml @19:10:26.85 delay 2 seconds @19:10:28.87 CBV.dial between PRV and PSV @19:10:29.22 PRV.dial :air @19:10:29.67 PS.seek 0.9ml @19:10:41.63 PRV.dial :waste2 @19:10:42.37 PS.seek 0.5ml @19:10:51.10 addPMLReagent08 @19:10:51.11 PSV.dial :air @19:10:51.58 PS.reconfigure :default @19:10:51.62 PS.seek 2ml @19:11:07.76 PSV.dial :PML @19:11:08.23 PTV.dial :mixing @19:11:08.98 PBV.dial :waste1 @19:11:09.43 PS.seek 1.85ml @19:11:11.88 PBV.dial between PSV and waste1 @19:11:12.26 PS.seek 0ml @19:11:32.04 delay 2 seconds @19:11:34.06 Beginning samplePassC @19:11:34.07 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:11:34.25 Elbow.reconfigure :tight @19:11:34.30 Elbow.seek FlushPuck::Garage @19:11:35.60 Elbow.reconfigure :loose @19:11:35.70 Forearm.seek :garage @19:11:36.72 Hand.close @19:11:37.62 Forearm.seek :retract @19:11:38.64 Elbow.reconfigure :tight @19:11:38.69 Elbow.seek CC @19:11:41.52 Elbow.reconfigure :loose @19:11:41.62 Forearm.reconfigure :tight @19:11:41.67 Forearm.seek CC @19:11:43.30 CC.seek :closed @19:11:47.28 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.97 @19:11:47.34 Hand.open @19:11:48.19 Forearm.reconfigure :loose @19:11:48.23 Forearm.seek :retract @19:11:49.27 PSV.dial PSR @19:11:50.30 Pulling 1.5ml of PSR.flush @19:11:50.31 PSR.select :flush @19:11:50.43 PS.seek 1.5ml @19:12:06.63 delay 5 seconds @19:12:11.65 PSV.dial :waste1 @19:12:12.11 PS.seek 0ml @19:12:28.23 PSV.dial PSR @19:12:28.69 PSR.select :air @19:12:28.81 PS.seek 2.5ml @19:12:55.10 PRV.dial CBV,avoiding: :ab2 @19:12:55.13
CBV.dial PRV @19:12:56.76 PSV.dial :mixloop @19:12:57.79 CTV.dial :air,avoiding: :intake @19:12:57.87 PTV.dial :PRVmixing @19:12:58.64 PS.seek 0ml @19:13:25.00 PSV.dial :air @19:13:25.48 PS.seek 2.5ml @19:13:51.88 PSV.dial :mixloop @19:13:52.35 PS.seek 0ml @19:14:18.63 PSV.dial :air @19:14:19.10 PS.seek 2.5ml @19:14:45.41 PSV.dial :mixloop @19:14:45.89 PS.seek 0ml @19:15:12.22 Garaging FlushPuck in Collection Clamp @19:15:12.29 Elbow.reconfigure :tight @19:15:12.34 Elbow.seek CC @19:15:12.91 Elbow.reconfigure :loose @19:15:13.01 Forearm.seek CC @19:15:14.04 Hand.close @19:15:14.75 CC.seek :open @19:15:18.85 Forearm.seek :retract @19:15:19.91 Elbow.reconfigure :tight @19:15:19.95 Elbow.seek :garage @19:15:22.76 Elbow.reconfigure :loose @19:15:22.85 Forearm.reconfigure :tight @19:15:22.90 Forearm.seek :garage @19:15:24.01 Hand.open @19:15:24.81 Forearm.reconfigure :loose @19:15:24.85 Forearm.seek :retract @19:15:25.95 delayUntil "19:19:34.05" @19:15:25.98 Whole Cell Archive sampling 1000ml @19:15:26.00 Loading wcr puck from tube 4 into Collection Clamp @19:15:26.23 SC.reconfigure :tight @19:15:26.28 SC.seek 4 @19:15:26.34 SC.configure :limp @19:15:26.56 Elbow.reconfigure :tight @19:15:26.61 Elbow.seek 4 @19:15:28.46 Forearm.reconfigure :tight @19:15:28.51 Forearm.seek 4 + 800 counts @19:15:29.52 SE.reconfigure :pick @19:15:29.62 SE.seek :beamBreak @19:15:30.97 SE.configure :default @19:15:31.13 Elbow.seek 4 @19:15:31.75 Forearm.seek 4 + 300 counts @19:15:32.15 Forearm.reconfigure :loose @19:15:32.20 Hand.close @19:15:33.01 SE.seek :down @19:15:33.21 Forearm.seek :retract @19:15:34.07 SE.stop @19:15:34.14 Elbow.seek CC @19:15:35.69 Elbow.reconfigure :loose @19:15:35.80 Forearm.reconfigure :tight @19:15:35.85 Forearm.seek CC @19:15:37.13 CC.seek :closed @19:15:41.14 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.96 @19:15:41.15 Hand.open @19:15:41.93 Forearm.reconfigure :loose @19:15:41.98 Forearm.seek :retract @19:15:42.97 collectSample 1000ml w/bubble point of 35psi @19:15:42.98 Priming 7ml @19:15:42.99 Intake.select :close @19:15:43.00 Exhaust.select :close @19:15:43.02 SS.hold @19:15:43.21 Calibrating pressure sensors @19:15:43.23 CTV.dial :air @19:15:43.24 SSV.dial :spit,avoiding: :exhaust @19:15:43.32 SS.reconfigure :fast @19:15:43.37 SS.seek 5ml @19:15:46.01 SSV.dial CBV @19:15:46.03 CBV.dial SSV @19:15:47.44 delay 3.8 seconds @19:15:51.83 Sensor limits: Intake max 99.8psia; Outlet max 109.0psia @19:15:51.84 SSV.dial :spit @19:15:53.20 SS.seek 0ml @19:15:55.80 SSV.dial CBV @19:15:57.15 CTV.dial :intake @19:15:57.94 SSV.dial CBV @19:15:57.96 CBV.dial SSV @19:15:58.11 Allowing 40s for 7.0ml of remaining 7.0ml @19:15:58.13 Intake.select :open @19:16:00.29 Intake @14.4psia, vacuum from 8.9 to 11.9psi @19:16:00.31 SS.reconfigure :inhaling 2.17ml/s @19:16:00.36 SS.seek 7ml @19:16:06.75 Stroke completed -- waiting for pressure to equalize ... @19:16:06.76 SS.reconfigure :exhale @19:16:06.81 SS.hold @19:16:25.17 Intake.select :close @19:16:25.32 SSV.dial :exhaust @19:16:26.10 Exhaust.select :open @19:16:26.22 SS.seek 0ml @19:16:30.24 Exhaust.select :close @19:16:30.35 Sampling 1000.0ml @19:16:30.36 Intake.select :close @19:16:30.37 Exhaust.select :close @19:16:30.39 SS.reconfigure :default @19:16:30.43 SS.hold @19:16:30.64 CTV.dial :intake @19:16:30.80 SSV.dial CBV @19:16:30.82 CBV.dial SSV @19:16:31.65 Allowing 130s for 25.0ml of remaining 1000.0ml @19:16:31.66 Intake.select :open @19:16:33.82 Intake @14.0psia, vacuum from 8.5 to 11.5psi @19:16:33.84 SS.reconfigure :inhaling 2.17ml/s @19:16:33.89 SS.seek 25ml @19:16:45.01 Vacuum too high -- waiting up to 40 seconds @19:16:45.03 SS.reconfigure :exhale @19:16:45.08 SS.hold @19:16:47.07 Intake @11.7psia, vacuum from 6.2 to 9.2psi @19:16:47.09 SS.reconfigure :inhaling 1.95ml/s @19:16:47.14 SS.seek 25ml @19:16:47.29 Vacuum too high -- waiting up to 40 seconds @19:16:47.31 SS.reconfigure :exhale @19:16:47.36 SS.hold @19:16:56.18 Intake @13.0psia, vacuum from 7.5 to 10.5psi @19:16:56.20 SS.reconfigure :inhaling 1.77ml/s @19:16:56.25 SS.seek 25ml @19:17:01.27 Stroke completed -- waiting for pressure to equalize ... @19:17:01.28 SS.reconfigure :exhale @19:17:01.33 SS.hold @19:17:09.27 Intake.select :close @19:17:09.43 SSV.dial :exhaust @19:17:10.21 Exhaust.select :open @19:17:10.33 SS.seek 0ml @19:17:19.63 Exhaust.select :close @19:17:19.74 SSV.dial CBV @19:17:19.78 CBV.dial SSV @19:17:20.54 Allowing 130s for 25.0ml of remaining 975.0ml @19:17:20.55 Intake.select :open @19:17:22.72 Intake @13.6psia, vacuum from 8.1 to 11.1psi @19:17:22.74 SS.reconfigure :inhaling 1.77ml/s @19:17:22.79 SS.seek 25ml @19:17:29.63 Vacuum too high -- waiting up to 40 seconds @19:17:29.65 SS.reconfigure :exhale @19:17:29.70 SS.hold @19:17:30.92 Intake @11.2psia, vacuum from 5.7 to 8.7psi @19:17:30.93 SS.reconfigure :inhaling 1.59ml/s @19:17:30.98 SS.seek 25ml @19:17:33.67 Vacuum too high -- waiting up to 40 seconds @19:17:33.68 SS.reconfigure :exhale @19:17:33.74 SS.hold @19:17:38.21 Intake @12.1psia, vacuum from 6.6 to 9.6psi @19:17:38.23 SS.reconfigure :inhaling 1.43ml/s @19:17:38.28 SS.seek 25ml @19:17:44.70 Vacuum too high -- waiting up to 40 seconds @19:17:44.72 SS.reconfigure :exhale @19:17:44.77 SS.hold @19:17:47.35 Intake @11.6psia, vacuum from 6.1 to 9.1psi @19:17:47.37 SS.reconfigure :inhaling 1.29ml/s @19:17:47.41 SS.seek 25ml @19:17:52.02 Stroke completed -- waiting for pressure to equalize ... @19:17:52.03 SS.reconfigure :exhale @19:17:52.08 SS.hold @19:17:56.94 Intake.select :close @19:17:57.10 SSV.dial :exhaust @19:17:57.86 Exhaust.select :open @19:17:57.97 SS.seek 0ml @19:18:07.28 Exhaust.select :close @19:18:07.40 SSV.dial CBV @19:18:07.42 CBV.dial SSV @19:18:08.24 Allowing 130s for 25.0ml of remaining 950.0ml @19:18:08.25 Intake.select :open @19:18:10.41 Intake @12.9psia, vacuum from 7.4 to 10.4psi @19:18:10.43 SS.reconfigure :inhaling 1.29ml/s @19:18:10.48 SS.seek 25ml @19:18:20.95 Vacuum too high -- waiting up to 40 seconds @19:18:20.96 SS.reconfigure :exhale @19:18:21.02 SS.hold @19:18:22.08 Intake @10.2psia, vacuum from 4.7 to 7.7psi @19:18:22.10 SS.reconfigure :inhaling 1.16ml/s @19:18:22.14 SS.seek 25ml @19:18:26.11 Vacuum too high -- waiting up to 40 seconds @19:18:26.12 SS.reconfigure :exhale @19:18:26.18 SS.hold @19:18:29.85 Intake @10.2psia, vacuum from 4.7 to 7.7psi @19:18:29.87 SS.reconfigure :inhaling 1.04ml/s @19:18:29.92 SS.seek 25ml @19:18:39.50 Stroke completed -- waiting for pressure to equalize ... @19:18:39.51 SS.reconfigure :exhale @19:18:39.56 SS.hold @19:18:45.25 Intake.select :close @19:18:45.41 SSV.dial :exhaust @19:18:46.17 Exhaust.select :open @19:18:46.29 SS.seek 0ml @19:18:55.67 Exhaust.select :close @19:18:55.78 SSV.dial CBV @19:18:55.82 CBV.dial SSV @19:18:56.58 Allowing 130s for 25.0ml of remaining 925.0ml @19:18:56.59 Intake.select :open @19:18:58.75 Intake @13.4psia, vacuum from 7.9 to 10.9psi @19:18:58.77 SS.reconfigure :inhaling 1.04ml/s @19:18:58.82 SS.seek 25ml @19:19:10.82 Vacuum too high -- waiting up to 40 seconds @19:19:10.84 SS.reconfigure :exhale @19:19:10.89 SS.hold @19:19:13.01 Intake @11.7psia, vacuum from 6.2 to 9.2psi @19:19:13.03 SS.reconfigure :inhaling 0.94ml/s @19:19:13.08 SS.seek 25ml @19:19:19.88 Vacuum too high -- waiting up to 40 seconds @19:19:19.90 SS.reconfigure :exhale @19:19:19.95 SS.hold @19:19:23.30 Intake @12.3psia, vacuum from 6.8 to 9.8psi @19:19:23.32 SS.reconfigure :inhaling 0.85ml/s @19:19:23.37 SS.seek 25ml @19:19:32.95 Stroke completed -- waiting for pressure to equalize ... @19:19:32.96 SS.reconfigure :exhale @19:19:33.01 SS.hold @19:19:34.05 recoverPRVreagent08 @19:19:34.06 PTV.dial :mixing @19:19:34.81 PBV.dial :waste1 @19:19:35.20 delay 30 seconds @19:19:39.55 Intake.select :close @19:19:39.70 SSV.dial :exhaust @19:19:40.47 Exhaust.select :open @19:19:40.58 SS.seek 0ml @19:19:49.75 Exhaust.select :close @19:19:49.86 SSV.dial CBV @19:19:50.32 CBV.dial SSV @19:19:51.14 Allowing 130s for 25.0ml of remaining 900.0ml @19:19:51.15 Intake.select :open @19:19:53.32 Intake @13.8psia, vacuum from 8.3 to 11.3psi @19:19:53.34 SS.reconfigure :inhaling 0.85ml/s @19:19:53.39 SS.seek 25ml @19:20:04.88 Vacuum too high -- waiting up to 40 seconds @19:20:04.90 SS.reconfigure :exhale @19:20:04.95 SS.hold @19:20:05.30 PBV.dial between PSV and waste1 @19:20:05.70 PSV.dial :air @19:20:06.20 PS.reconfigure :fast @19:20:06.33 PS.seek 2.5ml @19:20:07.08 Intake @12.3psia, vacuum from 6.8 to 9.8psi @19:20:07.10 SS.reconfigure :inhaling 0.77ml/s @19:20:07.15 SS.seek 25ml @19:20:12.85 Vacuum too high -- waiting up to 40 seconds @19:20:12.87 SS.reconfigure :exhale @19:20:12.92 SS.hold @19:20:18.34 Intake @13.3psia, vacuum from 7.8 to 10.8psi @19:20:18.36 SS.reconfigure :inhaling 0.70ml/s @19:20:18.41 SS.seek 25ml @19:20:29.87 PSV.dial :mixloop @19:20:29.98 Vacuum too high -- waiting up to 40 seconds @19:20:29.99 SS.reconfigure :exhale @19:20:30.05 SS.hold @19:20:30.40 delay 2 seconds @19:20:32.43 PSV.dial :air @19:20:32.89 delay 2 seconds @19:20:34.97 PSV.dial :mixloop @19:20:35.44 delay 2 seconds @19:20:35.83 Intake @12.7psia, vacuum from 7.2 to 10.2psi @19:20:35.85 SS.reconfigure :inhaling 0.62ml/s @19:20:35.90 SS.seek 25ml @19:20:37.45 PSV.dial :air @19:20:37.93 PS.seek 0ml @19:20:43.92 Stroke completed -- waiting for pressure to equalize ... @19:20:43.93 SS.reconfigure :exhale @19:20:43.98 SS.hold @19:20:55.24 Intake.select :close @19:20:55.39 SSV.dial :exhaust @19:20:56.16 Exhaust.select :open @19:20:56.27 SS.seek 0ml @19:21:01.28 washCycleDA #1 of 2 @19:21:01.29 PSR.select :air @19:21:01.31 PSV.dial PSR @19:21:02.64 Pulling 0.5ml of PSR.wash @19:21:02.65 PSR.select :wash @19:21:02.77 PS.reconfigure :default @19:21:02.82 PS.seek 0.5ml @19:21:05.22 Exhaust.select :close @19:21:05.33 SSV.dial CBV @19:21:05.37 CBV.dial SSV @19:21:06.14 Allowing 130s for 25.0ml of remaining 875.0ml @19:21:06.15 Intake.select :open @19:21:08.31 Intake @14.1psia, vacuum from 8.6 to 11.6psi @19:21:08.33 SS.reconfigure :inhaling 0.62ml/s @19:21:08.38 SS.seek 25ml @19:21:08.78 delay 5 seconds @19:21:13.80 PSR.select :air @19:21:13.92 PS.seek 2ml @19:21:20.94 Vacuum too high -- waiting up to 40 seconds @19:21:20.96 SS.reconfigure :exhale @19:21:21.01 SS.hold @19:21:29.76 Intake @13.3psia, vacuum from 7.8 to 10.8psi @19:21:29.78 SS.reconfigure :inhaling 0.56ml/s @19:21:29.83 SS.seek 25ml @19:21:30.15 PTV.dial :puck @19:21:30.91 PSV.dial PTV @19:21:31.66 PBV.dial :waste1 @19:21:32.11 PS.seek 1.5ml @19:21:36.94 Vacuum too high -- waiting up to 40 seconds @19:21:36.96 SS.reconfigure :exhale @19:21:37.01 SS.hold @19:21:38.18 delay 3 seconds @19:21:41.63 PBV.dial between PSV and waste1 @19:21:42.04 PS.seek 0ml @19:21:46.81 Intake @13.3psia, vacuum from 7.8 to 10.8psi @19:21:46.83 SS.reconfigure :inhaling 0.51ml/s @19:21:46.88 SS.seek 25ml @19:21:55.88 Vacuum too high -- waiting up to 40 seconds @19:21:55.90 SS.reconfigure :exhale @19:21:55.95 SS.hold @19:21:58.34 delay 2:00 @19:22:05.91 Intake @13.4psia, vacuum from 7.9 to 10.9psi @19:22:06.36 SS.reconfigure :inhaling 0.46ml/s @19:22:06.43 SS.seek 25ml @19:22:17.54 Vacuum too high -- waiting up to 40 seconds @19:22:17.55 SS.reconfigure :exhale @19:22:17.61 SS.hold @19:22:38.14 Intake @13.6psia, vacuum from 8.1 to 11.1psi @19:22:38.16 SS.reconfigure :inhaling 0.42ml/s @19:22:38.20 SS.seek 25ml @19:22:50.53 Stroke completed -- waiting for pressure to equalize ... @19:22:50.54 SS.reconfigure :exhale @19:22:50.58 SS.hold @19:23:16.16 SS.reconfigure :default @19:23:16.21 SS.hold @19:23:16.28 Intake.select :close @19:23:16.43 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 150.0ml @19:23:16.45 SSV.dial :exhaust @19:23:17.22 Exhaust.select :open @19:23:17.79 SS.reconfigure :exhale @19:23:17.84 SS.seek 0ml @19:23:27.17 Exhaust.select :close @19:23:27.28 Sampled 150.0ml @19:23:27.30 Drying puck before fixing for 4:00 @19:23:27.31 CBV.dial :waste2b @19:23:28.05 delay 1:00 @19:23:58.36 recoverPSRreagent08 @19:23:58.37 PTV.dial :puck @19:23:58.45 PBV.dial :waste1 @19:23:58.84 delay 30 seconds @19:24:28.08 CSV.dial :air @19:24:28.54 CS.reconfigure :fast @19:24:28.59 CS.seek 10ml @19:24:28.86 PBV.dial between PSV and waste1 @19:24:29.23 PSR.select :air @19:24:29.25 PSV.dial PSR @19:24:30.00 PS.reconfigure :fast @19:24:30.05 PS.seek 2.5ml @19:24:52.35 Pumping wcr puck from tube 4 up to 35psi... @19:24:52.37 CBV.dial SSV @19:24:53.34 PSV.dial PTV @19:24:53.64 CBV.dial between CSV and waste2b @19:24:54.09 delay 5 seconds @19:24:54.61 CSV.dial between air and CTV @19:24:54.93 CS.seek 7.057ml @19:24:59.11 PSV.dial PSR @19:24:59.86 delay 5 seconds @19:25:02.48 CTV.dial CSV,avoiding: :air @19:25:02.51 CSV.dial CTV @19:25:03.34 CS.reconfigure :pressurize @19:25:03.39 CS.seek 0ml @19:25:04.87 PSV.dial PTV @19:25:05.62 delay 5 seconds @19:25:10.64 PSV.dial :air @19:25:11.43 PS.seek 0ml @19:25:21.09 CBV.dial :waste2b @19:25:21.43 CS.reconfigure :default @19:25:21.48 CS.seek 0ml @19:25:23.10 delay 1:00 @19:25:34.69 washCycleDA #2 of 2 @19:25:34.70 PSR.select :air @19:25:34.71 PSV.dial PSR @19:25:36.04 Pulling 0.5ml of PSR.wash @19:25:36.05 PSR.select :wash @19:25:36.17 PS.reconfigure :default @19:25:36.22 PS.seek 0.5ml @19:25:42.18 delay 5 seconds @19:25:47.20 PSR.select :air @19:25:47.32 PS.seek 2ml @19:26:03.46 PTV.dial :puck @19:26:03.91 PSV.dial PTV @19:26:04.67 PBV.dial :waste1 @19:26:05.10 PS.seek 1.5ml @19:26:11.06 delay 3 seconds @19:26:14.08 PBV.dial between PSV and waste1 @19:26:14.46 PS.seek 0ml @19:26:23.12 CTV.dial :air,avoiding: :intake @19:26:24.49 Fix #1 of 1 (with RNAlater) for 4:00 @19:26:24.51 CSV.dial CSR @19:26:24.94 Pulling 2ml of CSR.RNAlater @19:26:24.95 CSR.select :RNAlater @19:26:25.07 CS.seek 2ml @19:26:30.59 delay 2:00 @19:26:31.00 delay 5 seconds @19:26:36.02 CSR.select :air @19:26:36.14 CS.reconfigure :fast @19:26:36.19 CS.seek 10ml @19:26:55.38 Pumping wcr puck from tube 4 up to 35psi... @19:26:55.40 CBV.dial SSV @19:26:56.65 CBV.dial between CSV and waste2b @19:26:57.62 CSV.dial between air and CTV @19:26:58.23 CS.seek 7.024ml @19:27:05.94 CTV.dial CSV,avoiding: :air @19:27:05.97 CSV.dial CTV @19:27:07.40 CS.reconfigure :pressurize @19:27:07.45 CS.seek 0ml @19:27:23.06 delay 4:00 @19:28:30.62 recoverPSRreagent08 @19:28:30.63 PTV.dial :puck @19:28:30.64 PBV.dial :waste1 @19:28:31.01 delay 30 seconds @19:29:01.04 PBV.dial between PSV and waste1 @19:29:01.42 PSR.select :air @19:29:01.43 PSV.dial PSR @19:29:02.19 PS.reconfigure :fast @19:29:02.24 PS.seek 2.5ml @19:29:25.59 PSV.dial PTV @19:29:26.34 delay 5 seconds @19:29:31.36 PSV.dial PSR @19:29:32.10 delay 5 seconds @19:29:37.12 PSV.dial PTV @19:29:37.87 delay 5 seconds @19:29:42.89 PSV.dial :air @19:29:43.66 PS.seek 0ml @19:30:06.75 getPMLReagent08 @19:30:06.76 PRV.dial :air @19:30:07.21 delay 5 seconds @19:30:12.23 PTV.dial :PRVmixing @19:30:13.53 delay 5 seconds @19:30:18.55 PRV.dial :ab2 @19:30:19.47 PTV.dial :PRVmixing @19:30:19.54 PSV.dial :mixloop @19:30:20.01 PS.reconfigure :default @19:30:20.06 PS.seek 0.3ml @19:30:23.96 delay 2 seconds @19:30:25.98 PRV.dial :air @19:30:26.51 PS.seek 0.55ml @19:30:29.93 addPMLReagent08 @19:30:29.94 PSV.dial :air @19:30:30.41 PS.seek 2ml @19:30:46.02 PSV.dial :PML @19:30:46.49 PTV.dial :mixing @19:30:47.24 PBV.dial :waste1 @19:30:47.69 PS.seek 1.85ml @19:30:50.12 PBV.dial between PSV and waste1 @19:30:50.51 PS.seek 0ml @19:31:10.43 delay 2 seconds @19:31:12.45 delay 8:00 @19:31:23.07 CBV.dial :waste2b @19:31:23.41 CS.reconfigure :default @19:31:23.46 CS.seek 0ml @19:31:27.06 delay 1:00 @19:32:27.09 CTV.dial :air,avoiding: :intake @19:39:12.47 recoverPRVreagent08 @19:39:12.48 PTV.dial :mixing @19:39:12.66 PBV.dial :waste1 @19:39:13.05 delay 30 seconds @19:39:43.08 PBV.dial between PSV and waste1 @19:39:43.46 PSV.dial :air @19:39:43.93 PS.reconfigure :fast @19:39:43.98 PS.seek 2.5ml @19:40:07.45 PSV.dial :mixloop @19:40:07.92 delay 2 seconds @19:40:09.94 PSV.dial :air @19:40:10.41 delay 2 seconds @19:40:12.43 PSV.dial :mixloop @19:40:12.89 delay 2 seconds @19:40:14.91 PSV.dial :air @19:40:15.38 PS.seek 0ml @19:40:38.55 washCycleDA #1 of 3 @19:40:38.56 PSR.select :air @19:40:38.58 PSV.dial PSR @19:40:39.91 Pulling 0.5ml of PSR.wash @19:40:39.92 PSR.select :wash @19:40:40.04 PS.reconfigure :default @19:40:40.08 PS.seek 0.5ml @19:40:46.02 delay 5 seconds @19:40:51.04 PSR.select :air @19:40:51.16 PS.seek 2ml @19:41:07.26 PTV.dial :puck @19:41:08.02 PSV.dial PTV @19:41:08.75 PBV.dial :waste1 @19:41:09.19 PS.seek 1.5ml @19:41:15.20 delay 3 seconds @19:41:18.22 PBV.dial between PSV and waste1 @19:41:18.60 PS.seek 0ml @19:41:34.99 delay 2:00 @19:43:35.02 recoverPSRreagent08 PTV.dial :puck @19:43:35.09 PBV.dial :waste1 @19:43:35.47 delay 30 seconds @19:44:05.50 PBV.dial between PSV and waste1 @19:44:05.87 PSR.select :air @19:44:05.89 PSV.dial PSR @19:44:06.65 PS.reconfigure :fast @19:44:06.69 PS.seek 2.5ml @19:44:30.20 PSV.dial PTV @19:44:30.95 delay 5 seconds @19:44:35.97 PSV.dial PSR @19:44:36.83 delay 5 seconds @19:44:41.85 PSV.dial PTV @19:44:42.58 delay 5 seconds @19:44:47.60 PSV.dial :air @19:44:48.38 PS.seek 0ml @19:45:11.64 washCycleDA #2 of 3 @19:45:11.65 PSR.select :air @19:45:11.67 PSV.dial PSR @19:45:13.00 Pulling 0.5ml of PSR.wash @19:45:13.01 PSR.select :wash @19:45:13.13 PS.reconfigure :default @19:45:13.17 PS.seek 0.5ml @19:45:19.08 delay 5 seconds @19:45:24.10 PSR.select :air @19:45:24.22 PS.seek 2ml @19:45:40.32 PTV.dial :puck @19:45:40.34 PSV.dial PTV @19:45:41.08 PBV.dial :waste1 @19:45:41.51 PS.seek 1.5ml @19:45:47.76 delay 3 seconds @19:45:50.78 PBV.dial between PSV and waste1 @19:45:51.16 PS.seek 0ml @19:46:07.33 delay 2:00 @19:48:07.36 recoverPSRreagent08 @19:48:07.37 PTV.dial :puck @19:48:07.38 PBV.dial :waste1 @19:48:07.75 delay 30 seconds @19:48:37.77 PBV.dial between PSV and waste1 @19:48:38.15 PSR.select :air @19:48:38.17 PSV.dial PSR @19:48:38.92 PS.reconfigure :fast @19:48:38.97 PS.seek 2.5ml @19:49:02.51 PSV.dial PTV @19:49:03.26 delay 5 seconds @19:49:08.28 PSV.dial PSR @19:49:09.03 delay 5 seconds @19:49:14.05 PSV.dial PTV @19:49:14.79 delay 5 seconds @19:49:19.81 PSV.dial :air @19:49:20.58 PS.seek 0ml @19:49:43.70 washCycleDA #3 of 3 @19:49:43.71 PSR.select :air @19:49:43.73 PSV.dial PSR @19:49:45.06 Pulling 0.5ml of PSR.wash @19:49:45.07 PSR.select :wash @19:49:45.18 PS.reconfigure :default @19:49:45.23 PS.seek 0.5ml @19:49:51.17 delay 5 seconds @19:49:56.19 PSR.select :air @19:49:56.31 PS.seek 2ml @19:50:12.45 PTV.dial :puck @19:50:12.46 PSV.dial PTV @19:50:13.18 PBV.dial :waste1 @19:50:14.10 PS.seek 1.5ml @19:50:20.07 delay 3 seconds @19:50:23.09 PBV.dial between PSV and waste1 @19:50:23.47 PS.seek 0ml @19:50:39.60 delay 2:00 @19:52:39.63 recoverPSRreagent08 @19:52:39.64 PTV.dial :puck @19:52:39.65 PBV.dial :waste1 @19:52:40.02 delay 30 seconds @19:53:10.05 PBV.dial between PSV and waste1 @19:53:10.43 PSR.select :air @19:53:10.45 PSV.dial PSR @19:53:11.20 PS.reconfigure :fast @19:53:11.25 PS.seek 2.5ml @19:53:34.57 PSV.dial PTV @19:53:35.32 delay 5 seconds @19:53:40.34 PSV.dial PSR @19:53:41.09 delay 5 seconds @19:53:46.11 PSV.dial PTV @19:53:46.85 delay 5 seconds @19:53:51.87 PSV.dial :air @19:53:52.64 PS.seek 0ml @19:54:15.54 Gate.power :camera,:ON @19:54:15.55 Mixing DA Substrates @19:54:15.56 PSR.select :air @19:54:15.58 PSV.dial PSR @19:54:16.95 Pulling 0.25ml of PSR.sub1 @19:54:16.97 PSR.select :sub1 @19:54:17.17 PS.reconfigure :default @19:54:17.29 PS.seek 0.25ml @19:54:20.77 delay 5 seconds @19:54:25.79 Pulling 0.25ml of PSR.sub2 @19:54:25.80 PSR.select :sub2 @19:54:25.92 PS.seek 0.5ml @19:54:29.23 delay 5 seconds @19:54:34.25 PSR.select :air @19:54:34.36 PS.seek 2ml @19:54:50.51 PTV.dial :puck @19:54:50.52 PSV.dial PTV @19:54:51.27 PBV.dial :waste1 @19:54:51.72 PS.seek 1.5ml @19:54:57.73 delay 3 seconds @19:55:00.75 PBV.dial between PSV and waste1 @19:55:01.14 PS.seek 0ml @19:55:17.26 delay 10 seconds @19:55:27.29 recoverPSRreagent08 @19:55:27.30 PTV.dial :puck @19:55:27.31 PBV.dial :waste1 @19:55:27.69 delay 30 seconds @19:55:57.72 PBV.dial between PSV and waste1 @19:55:58.12 PSR.select :air @19:55:58.13 PSV.dial PSR @19:55:59.01 PS.reconfigure :fast @19:55:59.06 PS.seek 2.5ml @19:56:22.60 PSV.dial PTV @19:56:23.33 delay 5 seconds @19:56:28.35 PSV.dial PSR @19:56:29.10 delay 5 seconds @19:56:34.12 PSV.dial PTV @19:56:34.99 delay 5 seconds @19:56:40.01 PSV.dial :air @19:56:40.79 PS.seek 0ml @19:57:03.94 Additional air clear @19:57:03.95 PSR.select :air @19:57:03.97 PSV.dial PSR @19:57:05.29 Pulling 2ml of PSR.air @19:57:05.30 PSR.select :air @19:57:05.32 PS.reconfigure :default @19:57:05.36 PS.seek 2ml @19:57:27.12 delay 5 seconds @19:57:32.20 PTV.dial :puck @19:57:32.21 PSV.dial PTV @19:57:32.96 PBV.dial :waste1 @19:57:33.39 PS.seek 1.5ml @19:57:39.35 delay 3 seconds @19:57:42.37 PBV.dial between PSV and waste1 @19:57:42.74 delay 1 second @19:57:43.76 PSV.dial :waste1 @19:57:44.85 PS.seek 0ml @19:58:01.03 Processing Puck Heater at 28C before imaging @19:58:01.04 PH.coast @19:58:01.15 Imaging da puck in Processing Clamp while storing it to tube 3 @19:58:01.33 SC.reconfigure :tight @19:58:01.37 SC.seek 3 @19:58:05.03 SC.configure :limp @19:58:05.24 Elbow.reconfigure :tight @19:58:05.29 Elbow.seek 3 @19:58:07.01 Forearm.reconfigure :tight @19:58:07.06 Forearm.seek 3 + 800 counts @19:58:08.00 SE.reconfigure :pick @19:58:08.05 SE.seek :beamBreak @19:58:10.89 SE.configure :default @19:58:11.05 Elbow.reconfigure :limp @19:58:11.10 Forearm.reconfigure :loose @19:58:11.14 Forearm.seek 3 @19:58:11.53 Elbow.hold @19:58:11.59 Elbow.reconfigure :tight @19:58:11.69 SE.seek 1.1 Pucks below Top Plate @19:58:12.12 Forearm.force -20 @19:58:12.17 Forearm.seek 3 + 318 counts @19:58:12.44 Forearm.reconfigure :tight @19:58:12.49 Forearm.seek 3 + 800 counts @19:58:12.72 SE.seek 2.1 Pucks below Top Plate @19:58:13.27 Forearm.reconfigure :loose @19:58:13.32 Forearm.seek :retract @19:58:14.72 Elbow.seek PC @19:58:17.30 Elbow.reconfigure :loose @19:58:17.40 Forearm.seek PC @19:58:18.50 Hand.close @19:58:19.21 PC.seek :open @19:58:23.77 Forearm.seek :retract @19:58:24.77 Elbow.reconfigure :tight @19:58:24.82 Elbow.seek 3 @19:58:27.43 Forearm.reconfigure :tight @19:58:27.48 Forearm.seek 3 + 800 counts @19:58:28.50 Hand.open @19:58:29.26 SE.seek 1.86 Pucks below Top Plate @19:58:29.53 SE.reconfigure :seal @19:58:29.58 SE.seek 1.2 Pucks below Top Plate @19:58:30.13 Lights, camera, ACTION! @19:58:30.16 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da25nov1219h875ml.tif @19:58:49.64 Waiting >20 seconds for da to relinquish FlushPuck @19:58:54.60 SX HX9/16 (USB:11): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21.131 seconds /var/log/esp/da25nov1219h875ml.tif: TIFF Upload Complete @19:58:54.63 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da25nov1219h875ml15s.tif @19:59:10.36 SX HX9/16 (USB:11): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da25nov1219h875ml15s.tif: TIFF Upload Complete @19:59:10.39 PuckCamera: snap -bin=2,2 21.0 /var/log/esp/midres/da25nov1219h875ml21s.tif @19:59:32.04 SX HX9/16 (USB:11): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21 seconds /var/log/esp/midres/da25nov1219h875ml21s.tif: TIFF Upload Complete @19:59:32.06 Gate.power :camera,:OFF @19:59:32.09 DAfans.email "21.131 sec auto-exposure",Subject: "da25nov1219h875ml images!" @19:59:32.51 Elbow.seek 3 @19:59:32.83 Forearm.seek 3 + 800 counts @19:59:32.92 SE.reconfigure :gently @19:59:32.98 SE.seek 1.92 Pucks below Top Plate @19:59:33.65 Elbow.reconfigure :limp @19:59:33.72 Forearm.reconfigure :loose @19:59:33.77 Forearm.seek 3 @19:59:34.17 Elbow.hold @19:59:34.24 Elbow.reconfigure :tight @19:59:34.29 SE.reconfigure :default @19:59:34.34 SE.seek 2.1 Pucks below Top Plate @19:59:35.28 Forearm.force -20 @19:59:35.33 Forearm.seek 3 + 319 counts @19:59:35.96 Forearm.reconfigure :tight @19:59:36.02 Forearm.seek 3 + 800 counts @19:59:36.34 SE.seek :down @19:59:37.59 Forearm.reconfigure :loose @19:59:37.69 Forearm.seek :retract @19:59:38.91 SE.stop @19:59:39.14 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:59:39.67 Elbow.seek FlushPuck::Garage @19:59:41.56 Elbow.reconfigure :loose @19:59:41.76 Forearm.seek :garage @19:59:42.82 Hand.close @19:59:43.83 Forearm.seek :retract @19:59:44.94 Elbow.reconfigure :tight @19:59:45.04 Elbow.seek PC @19:59:46.43 Elbow.reconfigure :loose @19:59:46.63 Forearm.reconfigure :tight @19:59:46.74 Forearm.seek PC @19:59:48.46 PC.seek :closed @19:59:53.13 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.54 @19:59:53.27 Hand.open @19:59:54.23 Forearm.reconfigure :loose @19:59:55.40 Forearm.seek :retract @19:59:56.47 PSR.select :air @19:59:56.53 Storing wcr puck in Collection Clamp to tube 3 @19:59:56.59 PSV.dial PSR @19:59:57.07 SC.reconfigure :tight @19:59:57.17 SC.seek 3 @19:59:57.32 Pulling 0.5ml of PSR.flush @19:59:57.35 PSR.select :flush @19:59:57.47 SC.configure :limp @19:59:57.77 PS.seek 0.5ml @19:59:58.03 Elbow.reconfigure :tight @19:59:58.13 Elbow.seek 3 @20:00:01.01 Forearm.reconfigure :tight @20:00:01.11 Forearm.seek 3 + 800 counts @20:00:02.20 SE.reconfigure :pick @20:00:02.31 SE.seek :beamBreak @20:00:03.80 delay 5 seconds @20:00:05.28 SE.configure :default @20:00:05.61 Elbow.reconfigure :limp @20:00:05.72 Forearm.reconfigure :loose @20:00:05.81 Forearm.seek 3 @20:00:06.25 Elbow.hold @20:00:06.38 Elbow.reconfigure :tight @20:00:06.59 SE.seek 2.1 Pucks below Top Plate @20:00:07.54 Forearm.force -20 @20:00:07.63 Forearm.seek 3 + 315 counts @20:00:08.10 Forearm.reconfigure :tight @20:00:08.19 Forearm.seek 3 + 800 counts @20:00:08.55 SE.seek 3.1 Pucks below Top Plate @20:00:08.85 PSR.select :air @20:00:10.05 PS.seek 2.5ml @20:00:10.20 Forearm.reconfigure :loose @20:00:10.25 Forearm.seek :retract @20:00:10.35 upsync @20:00:11.16 Elbow.seek CC @20:00:12.72 Elbow.reconfigure :loose @20:00:12.83 Forearm.seek CC @20:00:13.86 Hand.close @20:00:14.58 CC.seek :open @20:00:18.80 Forearm.seek :retract @20:00:19.83 Elbow.reconfigure :tight @20:00:19.88 Elbow.seek 3 @20:00:21.60 Elbow.reconfigure :limp @20:00:21.65 Forearm.seek 3 @20:00:22.75 Elbow.hold @20:00:22.82 Elbow.reconfigure :tight @20:00:22.87 Hand.open @20:00:23.70 Forearm.force -20 @20:00:23.74 Forearm.seek 3 + 319 counts @20:00:24.04 Forearm.reconfigure :tight @20:00:24.09 Forearm.seek 3 + 800 counts @20:00:24.42 SE.seek :down @20:00:25.56 Forearm.reconfigure :loose @20:00:25.61 Forearm.seek :retract @20:00:26.87 SE.stop @20:00:26.91
Starting finalPurge @20:00:31.73 delay 1 second @20:00:32.75 PRV.dial :waste2,avoiding: :ab2 @20:00:34.63 PTV.dial :PRVmixing @20:00:35.91 PSV.dial :mixloop @20:00:36.94 PS.seek :empty @20:00:46.87
Waiting >20 seconds for psFlush to relinquish FlushPuck @20:01:03.47 PSR.select :air @20:01:03.49 PSV.dial PSR @20:01:04.53 Pulling 0.5ml of PSR.flush @20:01:04.55 PSR.select :flush @20:01:04.66 PS.seek 0.5ml @20:01:10.53 delay 5 seconds @20:01:15.55 PSR.select :air @20:01:15.67 PS.seek 2.5ml @20:01:36.97 delay 1 second @20:01:37.99 PBV.dial :waste1 @20:01:38.36 PTV.dial :mixing @20:01:39.10 PSV.dial :mixloop @20:01:40.15 PS.seek :empty @20:02:06.56 PSV.dial PSR @20:02:07.72 PSR.select :flush @20:02:07.84 PS.seek 1ml @20:02:18.84 PSR.select :air @20:02:18.96 PS.seek 2.5ml @20:02:35.12 PSV.dial PTV @20:02:35.86 PTV.dial :puck @20:02:36.61 PBV.dial :waste1 @20:02:36.63 PS.seek 0ml @20:03:02.97 PSV.dial PSR @20:03:03.72 PSR.select :flush @20:03:03.85 PS.seek 0.5ml @20:03:09.78 PSR.select :air @20:03:09.90 PS.seek 2.5ml @20:03:31.13 PSV.dial PTV @20:03:31.88 PBV.dial PSV @20:03:32.46 PS.seek 0ml @20:03:58.78 PSV.dial PBV @20:03:59.24 PS.seek 2.5ml @20:04:25.65 PSV.dial :waste1 @20:04:26.40 PS.seek 0ml @20:04:52.78 PSV.dial PSR @20:04:53.25 PSR.select :air @20:04:53.27 PS.seek 2.5ml @20:05:19.62 PSV.dial PTV @20:05:20.36 PBV.dial PSV @20:05:20.44 PS.seek 0ml @20:05:46.78 PSV.dial PBV @20:05:47.36 PS.seek 2.5ml @20:06:13.68 PSV.dial :waste1 @20:06:14.88 PS.seek 0ml @20:06:41.31 PSV.dial PSR @20:06:41.78 PSR.select :flush @20:06:41.90 PS.seek 1ml @20:06:52.93 PSR.select :air @20:06:53.05 PS.seek 2.5ml @20:07:09.25 PSV.dial PTV @20:07:09.98 PTV.dial :puck @20:07:10.06 PBV.dial :waste1 @20:07:10.64 PS.seek 0ml @20:07:37.03 PSV.dial PSR @20:07:37.78 PSR.select :flush @20:07:37.90 PS.seek 0.5ml @20:07:43.84 PSR.select :air @20:07:43.96 PS.seek 2.5ml @20:08:05.25 PSV.dial PTV @20:08:06.00 PBV.dial PSV @20:08:06.58 PS.seek 0ml @20:08:32.93 PSV.dial PBV @20:08:33.39 PS.seek 2.5ml @20:08:59.84 PSV.dial :waste1 @20:09:00.58 PS.seek 0ml @20:09:26.93 PSV.dial PSR @20:09:27.40 PSR.select :air @20:09:27.43 PS.seek 2.5ml @20:09:53.87 PSV.dial PTV @20:09:54.61 PBV.dial PSV @20:09:54.69 PS.seek 0ml @20:10:21.03 PSV.dial PBV @20:10:21.61 PS.seek 2.5ml @20:10:47.96 PSV.dial :waste1 @20:10:48.72 PS.seek 0ml @20:11:15.13
Loading FlushPuck into Collection Clamp from Processing Clamp @20:11:15.31 Elbow.seek PC @20:11:17.79 Elbow.reconfigure :loose @20:11:17.89 Forearm.seek PC @20:11:18.94 Hand.close @20:11:19.69 PC.seek :open @20:11:24.65 Forearm.seek :retract @20:11:25.66 Elbow.reconfigure :tight @20:11:25.71 Elbow.seek CC @20:11:29.30 Elbow.reconfigure :loose @20:11:29.40 Forearm.reconfigure :tight @20:11:29.44 Forearm.seek CC @20:11:30.77 CC.seek :closed @20:11:34.78 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.96 @20:11:34.84 Hand.open @20:11:35.63 Forearm.reconfigure :loose @20:11:35.67 Forearm.seek :retract @20:11:36.72 Flushing the Collection Valve::Manifold @20:11:36.84 CSV.dial CSR @20:11:37.28 Pulling 5ml of CSR.flush @20:11:37.29 CSR.select :flush @20:11:37.40 CS.seek 5ml @20:11:51.09 delay 5 seconds @20:11:56.11 CSR.select :air @20:11:56.28 CS.reconfigure :fast @20:11:56.32 CS.seek 7.5ml @20:12:02.96 CSV.dial :waste @20:12:04.36 CS.reconfigure :default @20:12:04.40 CS.seek 0ml @20:12:24.47 Flushing lower manifold's waste1 port @20:12:24.58 CSV.dial :flush @20:12:25.03 CS.reconfigure :fast @20:12:25.08 CS.seek 5ml @20:12:37.40 delay 5 seconds @20:12:42.42 CSV.dial :air @20:12:42.92 CS.seek 7ml @20:12:48.34 CSV.dial CTV @20:12:48.37
CTV.dial CSV @20:12:49.78 CBV.dial :waste1 @20:12:50.55 CS.seek 0ml @20:13:07.79 Flushing lower manifold's waste2 port @20:13:07.92 CSV.dial :flush @20:13:08.66 CS.seek 5ml @20:13:21.18 delay 5 seconds @20:13:26.20 CSV.dial :air @20:13:26.71 CS.seek 6.999ml @20:13:32.22 CSV.dial CTV @20:13:32.24
CTV.dial CSV @20:13:32.70 CBV.dial :waste2 @20:13:33.75 CS.seek 0ml @20:13:50.56 Flushing lower manifold's waste2b port @20:13:50.68 CSV.dial :flush @20:13:51.42 CS.seek 5ml @20:14:03.68 delay 5 seconds @20:14:08.70 CSV.dial :air @20:14:09.21 CS.seek 7ml @20:14:14.81 CSV.dial CTV @20:14:14.84
CTV.dial CSV @20:14:15.29 CBV.dial :waste2b @20:14:16.34 CS.seek 0ml @20:14:33.06 Disinfecting external Intake with kill @20:14:33.18 CSV.dial CSR @20:14:33.60 Pulling 8ml of CSR.kill @20:14:33.61 CSR.select :kill @20:14:33.73 CS.seek 8ml @20:14:52.96 delay 5 seconds @20:14:57.98 CSR.select :air @20:14:58.10 CS.seek 10ml @20:15:03.56 CSV.dial CBV @20:15:03.59
CBV.dial CSV @20:15:04.65 CTV.dial :intake @20:15:05.43 Intake.select :open @20:15:05.54 CS.seek 2ml @20:15:24.62 Intake.select :close @20:15:24.72 CTV.dial :air @20:15:25.48 CS.reconfigure :default @20:15:25.53 CS.seek 0ml @20:15:31.49 CSV.dial CSR @20:15:32.51 Pulling 5ml of CSR.flush @20:15:32.52 CSR.select :flush @20:15:32.63 CS.seek 5ml @20:15:46.31 delay 5 seconds @20:15:51.32 CSR.select :air @20:15:51.44 CS.seek 10ml @20:16:05.05 CSV.dial CBV @20:16:06.19 CS.seek 0ml @20:16:32.62 Garaging FlushPuck in Collection Clamp @20:16:32.69 Elbow.reconfigure :tight @20:16:33.17 Elbow.seek CC @20:16:33.35 Elbow.reconfigure :loose @20:16:33.45 Forearm.seek CC @20:16:34.48 Hand.close @20:16:35.19 CC.seek :open @20:16:39.26 Forearm.seek :retract @20:16:40.25 Elbow.reconfigure :tight @20:16:40.30 Elbow.seek :garage @20:16:43.15 Elbow.reconfigure :loose @20:16:43.24 Forearm.reconfigure :tight @20:16:43.29 Forearm.seek :garage @20:16:44.35 Hand.open @20:16:45.12 Forearm.reconfigure :loose @20:16:45.16 Forearm.seek :retract @20:16:46.14 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 17:45:47 @20:16:46.19 Asleep after completing DA_WCR_111225 assays @20:16:46.21 GoodNews.email "{1 => 22,\n 2 => 22,\n 3 => 4,\n 4 => 18,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@20:15:14, 41% flow, 19.4C, 26% humidity, 14.6psia, 13.70V, 0.453A avg, 292.885Ah, 6.21W\nSampled 150.0ml",Subject: "Asleep after completing DA_WCR_111225 assays" @20:16:50.12 upsync + ESPwhich DA_WCR_112025.rb + fn=DA_WCR_112025.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_112025.rb + [ -f /home/esp/esp2/mission/DA_WCR_112025.rb ] + realpath /home/esp/esp2/mission/DA_WCR_112025.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_112025.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_112025.rb @13:40:17.58UTC20-Nov-25
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:40:20.32 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @13:40:23.96 Polling Can every 10:00 @13:40:24.06 Cleanly exited at 13:39:54UTC20-Nov-25 @13:40:24.11 Loading /home/esp/esp2local/mission/DA_WCR_112025.rb mission @13:40:24.16 GoodNews.email "Starting DA_WCR_112025 w/pucks in tube 4\n{1 => 22,\n 2 => 22,\n 3 => 4,\n 4 => 18,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@13:40:23, 42% flow, 18.5C, 26% humidity, 14.5psia, 13.72V, 0.303A avg, 330.764Ah, 4.15W",Subject: "DA_WCR_112025 begun" @13:40:24.19
Preparing to sleep until + 00:00:05 @13:40:24.24 Asleep til + 00:00:05 @13:40:24.25 Continuing at 13:40 @13:40:24.54 Gate.power :core,:OFF @13:40:24.55 Gate.power :raw,:OFF @13:40:24.56 Shutdown @13:40:26.92 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112025.rb:14\n{1 => 22,\n 2 => 22,\n 3 => 4,\n 4 => 18,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@13:40:24, 42% flow, 18.5C, 26% humidity, 14.5psia, 13.72V, 0.303A avg, 330.764Ah, 4.15W",Subject: "Asleep until 13:40" @13:40:28.33 upsync @13:40:29.15
Powering core bus @13:40:30.98 upsync @13:40:34.78
Gate.power :raw,:ON @13:40:35.85 CS.configure :default @13:40:35.90 PS.configure :default @13:40:35.95 SS.configure :default @13:40:36.00 CC.configure :default @13:40:36.05 PC.configure :default @13:40:36.12 Forearm.configure :loose @13:40:36.17 Elbow.configure :loose @13:40:36.23 SC.configure :loose @13:40:36.28 SE.configure :default @13:40:36.33 CH.configure :default @13:40:36.37 PH.configure :default @13:40:36.40 All dwarf controllers running firmware version 3.85 @13:40:36.51 Valve.reset @13:40:36.82 PSV.dial :air @13:40:36.85 Intake.select :close @13:40:36.87 Exhaust.select :close @13:40:36.91 CTV.dial :air @13:40:37.03 CSV.dial :waste1 @13:40:37.10
Forearm.home.seek :retract @13:40:37.50 SSV.dial :spit,avoiding: :exhaust @13:40:37.89 PS.home.seek 0ml @13:40:37.96 Hand.close @13:40:38.00
Elbow.reconfigure :loose @13:40:38.09 Elbow.jog 1500 @13:40:38.37 CS.reconfigure :fast @13:40:38.41 SS.home.seek 0ml @13:40:38.51 CS.home.seek 0ml @13:40:38.58
Elbow.reconfigure :loose @13:40:38.63 Elbow.home.seek :clear @13:40:41.87 SE.home.seek :down @13:40:42.49 SC.reconfigure :tight @13:40:42.54 SC.home.seek :initial @13:40:47.01 PS.stop @13:40:58.44
SC.configure :limp @13:40:58.49 Forearm.seek :retract @13:40:58.56 Elbow.seek :hammer @13:40:59.61 Forearm.reconfigure :tight @13:40:59.66 Forearm.seek retracted @13:41:00.61 PC.home.seek :open @13:41:00.63
CC.home.seek :open @13:41:03.78 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:41:03.79 Notice.email "At 13:41:03 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112025.rb:14\n{1 => 22,\n 2 => 22,\n 3 => 4,\n 4 => 18,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@13:41:03, 42% flow, 18.6C, 26% humidity, 14.5psia, 13.74V, 0.259A avg, 330.769Ah, 3.55W",Subject: "Started DA_WCR_112025 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:41:03.81
Starting initialPurge @13:41:03.83 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:41:03.91 Hand.open @13:41:04.86 Elbow.reconfigure :tight @13:41:04.91 Elbow.seek FlushPuck::Garage @13:41:06.76 Elbow.reconfigure :loose @13:41:06.87 Forearm.reconfigure :loose @13:41:06.93 Forearm.seek :garage @13:41:07.77 upsync @13:41:07.95
Hand.close @13:41:08.85 Forearm.seek :retract @13:41:09.91 Elbow.reconfigure :tight @13:41:09.96 Elbow.seek CC @13:41:12.80 Elbow.reconfigure :loose @13:41:12.91 Forearm.reconfigure :tight @13:41:12.96 Forearm.seek CC @13:41:14.31 CC.seek :closed @13:41:18.29 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 2.01 @13:41:18.35 Hand.open @13:41:19.15 Forearm.reconfigure :loose @13:41:19.20 Forearm.seek :retract @13:41:20.23 Flushing the Collection Valve::Manifold @13:41:20.36 CSV.dial CSR @13:41:21.64 Pulling 5ml of CSR.flush CSR.select :flush @13:41:21.76 CS.reconfigure :default @13:41:21.81 CS.seek 5ml @13:41:35.87 delay 5 seconds @13:41:40.89 CSR.select :air @13:41:41.07 CS.reconfigure :fast @13:41:41.11 CS.seek 7.5ml @13:41:47.77 CSV.dial :waste @13:41:49.06 CS.reconfigure :default @13:41:49.11 CS.seek 0ml @13:42:09.31 Purging external Intake @13:42:09.43 CSV.dial :flush @13:42:09.87 CS.reconfigure :fast @13:42:09.92 CS.seek 10ml @13:42:33.90 delay 5 seconds @13:42:38.92 CSV.dial CBV @13:42:38.95
CBV.dial CSV @13:42:39.71 CTV.dial :intake @13:42:40.49 Intake.select :open @13:42:40.60 CS.seek 0ml @13:43:04.46 Intake.select :close @13:43:04.57 CTV.dial :air @13:43:05.34 Flushing upper manifold @13:43:05.46 CSV.dial :flush @13:43:06.20 CS.seek 5ml @13:43:18.77 delay 5 seconds @13:43:23.79 CSV.dial :air @13:43:24.30 CS.seek 6.999ml @13:43:29.78 CSV.dial CBV @13:43:29.80
CBV.dial CSV @13:43:30.84 CTV.dial :air @13:43:30.92 CS.seek 0ml @13:43:47.84 delay 7 seconds @13:43:54.86 CS.seek 3ml @13:44:02.71 CSV.dial :waste @13:44:03.17 CS.seek 0ml @13:44:11.04 CSV.dial :flush @13:44:11.48 CS.seek 3ml @13:44:19.34 delay 5 seconds @13:44:24.36 CSV.dial :air @13:44:24.87 CS.seek 5ml @13:44:30.40 CSV.dial :air @13:44:30.48 CS.seek 0ml @13:44:42.87 delay 7 seconds @13:44:49.89 CS.seek 3ml @13:44:57.71 CSV.dial :waste @13:44:58.45 CS.seek 0ml @13:45:06.24 Garaging FlushPuck in Collection Clamp @13:45:06.31 Elbow.reconfigure :tight @13:45:06.36 Elbow.seek CC @13:45:06.56 Elbow.reconfigure :loose @13:45:06.67 Forearm.seek CC @13:45:07.65 Hand.close @13:45:08.34 CC.seek :open @13:45:12.49 Forearm.seek :retract @13:45:13.46 Elbow.reconfigure :tight @13:45:13.51 Elbow.seek :garage @13:45:16.38 Elbow.reconfigure :loose @13:45:16.48 Forearm.reconfigure :tight @13:45:16.52 Forearm.seek :garage @13:45:17.58 Hand.open @13:45:18.40 Forearm.reconfigure :loose @13:45:18.44 Forearm.seek :retract @13:45:19.50 da sampling at most 1000ml, wcr at most 1000ml @13:45:19.53 DAfans.email "Started da in tube 4 at 13:45:19UTC20-Nov-25\n{1 => 22,\n 2 => 22,\n 3 => 4,\n 4 => 18,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@13:41:03, 42% flow, 18.6C, 26% humidity, 14.5psia, 13.74V, 0.259A avg, 330.769Ah, 3.55W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:45:19.56
Loading sh1 puck from tube 4 into Collection Clamp @13:45:19.84 SC.reconfigure :tight @13:45:19.89 SC.seek 4 @13:45:19.96 SC.configure :limp @13:45:20.22 Elbow.reconfigure :tight @13:45:20.27 Elbow.seek 4 @13:45:22.39 Forearm.reconfigure :tight @13:45:22.50 Forearm.seek 4 + 800 counts @13:45:23.58 SE.reconfigure :pick @13:45:23.64 SE.seek :beamBreak @13:45:23.68 upsync @13:45:25.07
SE.configure :default @13:45:25.24 Elbow.seek 4 @13:45:25.43 Forearm.seek 4 + 300 counts @13:45:25.60 Forearm.reconfigure :loose @13:45:25.65 Hand.close @13:45:26.35 SE.seek :down @13:45:26.99
Forearm.seek :retract @13:45:27.51 SE.stop @13:45:27.95
Elbow.seek CC @13:45:29.50 Elbow.reconfigure :loose @13:45:29.61 Forearm.reconfigure :tight @13:45:29.65 Forearm.seek CC @13:45:30.92 CC.seek :closed @13:45:34.90 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.98 @13:45:34.91 Hand.open @13:45:35.77 Forearm.reconfigure :loose @13:45:35.82 Forearm.seek :retract @13:45:36.81 collectSample 1000ml w/bubble point of 24psi @13:45:36.82 Priming 7ml @13:45:36.83 Intake.select :close @13:45:36.84 Exhaust.select :close @13:45:36.85 SS.hold @13:45:37.06 Calibrating pressure and temperature sensors @13:45:37.09 CTV.dial :air @13:45:37.10 SSV.dial :spit,avoiding: :exhaust @13:45:37.19 SS.reconfigure :fast @13:45:37.24 SS.seek 5ml @13:45:40.39 SSV.dial CBV @13:45:40.43
CBV.dial SSV @13:45:41.78 delay 3.8 seconds @13:45:45.72 Sensor limits: Intake max 92.8psia; Outlet max 109.0psia @13:45:45.73 SSV.dial :spit @13:45:47.07 SS.seek 0ml @13:45:49.61 SSV.dial CBV @13:45:50.97 CTV.dial :intake @13:45:51.74 SSV.dial CBV @13:45:51.77
CBV.dial SSV @13:45:51.90 Allowing 40s for 7.0ml of remaining 7.0ml @13:45:51.91 Intake.select :open @13:45:54.08 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:45:54.10 SS.reconfigure :inhaling 2.17ml/s @13:45:54.15 SS.seek 7ml @13:46:01.46 Stroke completed -- waiting for pressure to equalize ... @13:46:01.47 SS.reconfigure :exhale @13:46:01.52 SS.hold @13:46:03.22 Intake.select :close @13:46:03.38 SSV.dial :exhaust @13:46:04.16 Exhaust.select :open @13:46:04.27 SS.seek 0ml @13:46:08.36 Exhaust.select :close @13:46:08.47 Sampling 1000.0ml @13:46:08.48 Intake.select :close @13:46:08.49 Exhaust.select :close @13:46:08.50 SS.reconfigure :default @13:46:08.55 SS.hold @13:46:08.75 CTV.dial :intake @13:46:08.93 SSV.dial CBV @13:46:08.95
CBV.dial SSV @13:46:09.78 Allowing 130s for 25.0ml of remaining 1000.0ml @13:46:09.79 Intake.select :open @13:46:11.95 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:46:11.97 SS.reconfigure :inhaling 2.17ml/s @13:46:12.02 SS.seek 25ml @13:46:26.45 Stroke completed -- waiting for pressure to equalize ... @13:46:26.46 SS.reconfigure :exhale @13:46:26.51 SS.hold @13:46:35.07 Intake.select :close @13:46:35.23 SSV.dial :exhaust @13:46:36.00 Exhaust.select :open @13:46:36.11 SS.seek 0ml @13:46:45.45 Exhaust.select :close @13:46:45.56 SSV.dial CBV @13:46:45.58
CBV.dial SSV @13:46:46.39 Allowing 130s for 25.0ml of remaining 975.0ml @13:46:46.40 Intake.select :open @13:46:48.56 Intake @13.7psia, vacuum from 8.2 to 11.2psi @13:46:48.58 SS.reconfigure :inhaling 2.17ml/s @13:46:48.63 SS.seek 25ml @13:47:02.15 Vacuum too high -- waiting up to 40 seconds @13:47:02.16 SS.reconfigure :exhale @13:47:02.22 SS.hold @13:47:02.76 Intake @9.6psia, vacuum from 4.1 to 7.1psi @13:47:02.78 SS.reconfigure :inhaling 1.95ml/s @13:47:02.83 SS.seek 25ml @13:47:03.02 Vacuum too high -- waiting up to 40 seconds @13:47:03.04 SS.reconfigure :exhale @13:47:03.09 SS.hold @13:47:10.67 Intake @11.6psia, vacuum from 6.1 to 9.1psi @13:47:10.69 SS.reconfigure :inhaling 1.77ml/s @13:47:10.73 SS.seek 25ml @13:47:11.64 Stroke completed -- waiting for pressure to equalize ... @13:47:11.65 SS.reconfigure :exhale @13:47:11.69 SS.hold @13:47:12.03 Intake.select :close @13:47:12.20 SSV.dial :exhaust @13:47:12.97 Exhaust.select :open @13:47:13.08 SS.seek 0ml @13:47:22.28 Exhaust.select :close @13:47:22.39 SSV.dial CBV @13:47:22.41
CBV.dial SSV @13:47:23.20 Allowing 130s for 25.0ml of remaining 950.0ml @13:47:23.21 Intake.select :open @13:47:25.37 Intake @13.7psia, vacuum from 8.2 to 11.2psi @13:47:25.39 SS.reconfigure :inhaling 1.77ml/s @13:47:25.44 SS.seek 25ml @13:47:38.86 Vacuum too high -- waiting up to 40 seconds @13:47:38.87 SS.reconfigure :exhale @13:47:38.93 SS.hold @13:47:39.54 Intake @9.5psia, vacuum from 4.0 to 7.0psi @13:47:39.56 SS.reconfigure :inhaling 1.59ml/s @13:47:39.61 SS.seek 25ml @13:47:40.14 Vacuum too high -- waiting up to 40 seconds @13:47:40.15 SS.reconfigure :exhale @13:47:40.20 SS.hold @13:47:45.14 Intake @12.5psia, vacuum from 7.0 to 10.0psi @13:47:45.16 SS.reconfigure :inhaling 1.43ml/s @13:47:45.21 SS.seek 25ml @13:47:50.18 Stroke completed -- waiting for pressure to equalize ... @13:47:50.19 SS.reconfigure :exhale @13:47:50.24 SS.hold @13:47:53.12 Intake.select :close @13:47:53.27 SSV.dial :exhaust @13:47:54.04 Exhaust.select :open @13:47:54.15 SS.seek 0ml @13:48:03.03 Exhaust.select :close @13:48:03.14 SSV.dial CBV @13:48:03.16
CBV.dial SSV @13:48:03.95 Allowing 130s for 25.0ml of remaining 925.0ml @13:48:03.96 Intake.select :open @13:48:06.12 Intake @14.1psia, vacuum from 8.6 to 11.6psi @13:48:06.14 SS.reconfigure :inhaling 1.43ml/s @13:48:06.19 SS.seek 25ml @13:48:19.67 Vacuum too high -- waiting up to 40 seconds @13:48:19.69 SS.reconfigure :exhale @13:48:19.74 SS.hold @13:48:20.26 Intake @9.8psia, vacuum from 4.3 to 7.3psi @13:48:20.28 SS.reconfigure :inhaling 1.29ml/s @13:48:20.33 SS.seek 25ml @13:48:27.42 Stroke completed -- waiting for pressure to equalize ... @13:48:27.43 SS.reconfigure :exhale @13:48:27.48 SS.hold @13:48:31.66 Intake.select :close @13:48:31.81 SSV.dial :exhaust @13:48:32.60 Exhaust.select :open @13:48:32.71 SS.seek 0ml @13:48:41.99 Exhaust.select :close @13:48:42.10 SSV.dial CBV @13:48:42.12
CBV.dial SSV @13:48:42.93 Allowing 130s for 25.0ml of remaining 900.0ml @13:48:42.94 Intake.select :open @13:48:45.10 Intake @13.6psia, vacuum from 8.1 to 11.1psi @13:48:45.12 SS.reconfigure :inhaling 1.29ml/s @13:48:45.17 SS.seek 25ml @13:48:59.30 Vacuum too high -- waiting up to 40 seconds @13:48:59.31 SS.reconfigure :exhale @13:48:59.37 SS.hold @13:49:00.11 Intake @10.8psia, vacuum from 5.3 to 8.3psi @13:49:00.12 SS.reconfigure :inhaling 1.16ml/s @13:49:00.17 SS.seek 25ml @13:49:04.75 Vacuum too high -- waiting up to 40 seconds @13:49:04.76 SS.reconfigure :exhale @13:49:04.81 SS.hold @13:49:07.88 Intake @11.6psia, vacuum from 6.1 to 9.1psi @13:49:07.90 SS.reconfigure :inhaling 1.04ml/s @13:49:07.95 SS.seek 25ml @13:49:12.35 Stroke completed -- waiting for pressure to equalize ... @13:49:12.36 SS.reconfigure :exhale @13:49:12.41 SS.hold @13:49:14.32 Intake.select :close @13:49:14.48 SSV.dial :exhaust @13:49:15.24 Exhaust.select :open @13:49:15.35 SS.seek 0ml @13:49:24.13 Exhaust.select :close @13:49:24.24 SSV.dial CBV @13:49:24.26
CBV.dial SSV @13:49:25.06 Allowing 130s for 25.0ml of remaining 875.0ml @13:49:25.07 Intake.select :open @13:49:27.24 Intake @13.8psia, vacuum from 8.3 to 11.3psi @13:49:27.26 SS.reconfigure :inhaling 1.04ml/s @13:49:27.31 SS.seek 25ml @13:49:46.92 Vacuum too high -- waiting up to 40 seconds @13:49:46.93 SS.reconfigure :exhale @13:49:46.99 SS.hold @13:49:47.68 Intake @10.8psia, vacuum from 5.3 to 8.3psi @13:49:47.70 SS.reconfigure :inhaling 0.94ml/s @13:49:47.75 SS.seek 25ml @13:49:53.98 Vacuum too high -- waiting up to 40 seconds @13:49:54.00 SS.reconfigure :exhale @13:49:54.05 SS.hold @13:49:57.12 Intake @11.8psia, vacuum from 6.3 to 9.3psi @13:49:57.14 SS.reconfigure :inhaling 0.85ml/s @13:49:57.19 SS.seek 25ml @13:49:58.01 Stroke completed -- waiting for pressure to equalize ... @13:49:58.02 SS.reconfigure :exhale @13:49:58.06 SS.hold @13:49:59.32 Intake.select :close @13:49:59.89 SSV.dial :exhaust @13:50:00.67 Exhaust.select :open @13:50:00.78 SS.seek 0ml @13:50:09.95 Exhaust.select :close @13:50:10.06 SSV.dial CBV @13:50:10.08
CBV.dial SSV @13:50:10.88 Allowing 130s for 25.0ml of remaining 850.0ml @13:50:10.90 Intake.select :open @13:50:13.06 Intake @14.1psia, vacuum from 8.6 to 11.6psi @13:50:13.08 SS.reconfigure :inhaling 0.85ml/s @13:50:13.13 SS.seek 25ml @13:50:29.11 Vacuum too high -- waiting up to 40 seconds @13:50:29.12 SS.reconfigure :exhale @13:50:29.18 SS.hold @13:50:31.29 Intake @12.4psia, vacuum from 6.9 to 9.9psi @13:50:31.31 SS.reconfigure :inhaling 0.77ml/s @13:50:31.36 SS.seek 25ml @13:50:42.51 Vacuum too high -- waiting up to 40 seconds @13:50:42.53 SS.reconfigure :exhale @13:50:42.58 SS.hold @13:50:46.22 Intake @12.0psia, vacuum from 6.5 to 9.5psi @13:50:46.23 SS.reconfigure :inhaling 0.70ml/s @13:50:46.28 SS.seek 25ml @13:50:52.18 Stroke completed -- waiting for pressure to equalize ... @13:50:52.19 SS.reconfigure :exhale @13:50:52.23 SS.hold @13:50:55.86 Intake.select :close @13:50:56.02 SSV.dial :exhaust @13:50:56.79 Exhaust.select :open @13:50:56.90 SS.seek 0ml @13:51:06.19 Exhaust.select :close @13:51:06.30 SSV.dial CBV @13:51:06.32
CBV.dial SSV @13:51:07.15 Allowing 130s for 25.0ml of remaining 825.0ml @13:51:07.16 Intake.select :open @13:51:09.33 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:51:09.34 SS.reconfigure :inhaling 0.70ml/s @13:51:09.39 SS.seek 25ml @13:51:22.48 Vacuum too high -- waiting up to 40 seconds @13:51:22.50 SS.reconfigure :exhale @13:51:22.55 SS.hold @13:51:25.62 Intake @12.6psia, vacuum from 7.1 to 10.1psi @13:51:25.64 SS.reconfigure :inhaling 0.62ml/s @13:51:25.69 SS.seek 25ml @13:51:31.79 Vacuum too high -- waiting up to 40 seconds @13:51:31.81 SS.reconfigure :exhale @13:51:31.86 SS.hold @13:51:37.25 Intake @12.7psia, vacuum from 7.2 to 10.2psi @13:51:37.27 SS.reconfigure :inhaling 0.56ml/s @13:51:37.32 SS.seek 25ml @13:51:53.14 Vacuum too high -- waiting up to 40 seconds @13:51:53.15 SS.reconfigure :exhale @13:51:53.21 SS.hold @13:51:58.17 SS.reconfigure :inhaling 0.51ml/s @13:51:58.22 SS.seek 25ml @13:52:07.16 Stroke completed -- waiting for pressure to equalize ... @13:52:07.17 SS.reconfigure :exhale @13:52:07.22 SS.hold @13:52:12.85 Intake.select :close @13:52:13.01 SSV.dial :exhaust @13:52:13.77 Exhaust.select :open @13:52:13.88 SS.seek 0ml @13:52:23.15 Exhaust.select :close @13:52:23.25 SSV.dial CBV @13:52:23.28
CBV.dial SSV @13:52:24.07 Allowing 130s for 25.0ml of remaining 800.0ml @13:52:24.08 Intake.select :open @13:52:26.25 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:52:26.27 SS.reconfigure :inhaling 0.51ml/s @13:52:26.31 SS.seek 25ml @13:52:42.26 Vacuum too high -- waiting up to 40 seconds @13:52:42.28 SS.reconfigure :exhale @13:52:42.33 SS.hold @13:52:49.91 Intake @12.7psia, vacuum from 7.2 to 10.2psi @13:52:49.92 SS.reconfigure :inhaling 0.46ml/s @13:52:49.97 SS.seek 25ml @13:52:58.54 Vacuum too high -- waiting up to 40 seconds @13:52:58.56 SS.reconfigure :exhale @13:52:58.61 SS.hold @13:53:11.41 Intake @12.5psia, vacuum from 7.0 to 10.0psi @13:53:11.43 SS.reconfigure :inhaling 0.42ml/s @13:53:11.48 SS.seek 25ml @13:53:24.76 Vacuum too high -- waiting up to 40 seconds @13:53:24.77 SS.reconfigure :exhale @13:53:24.83 SS.hold @13:53:37.63 Intake @12.3psia, vacuum from 6.8 to 9.8psi @13:53:37.64 SS.reconfigure :inhaling 0.38ml/s @13:53:37.69 SS.seek 25ml @13:53:57.23 Vacuum too high -- waiting up to 40 seconds @13:53:57.24 SS.reconfigure :exhale @13:53:57.29 SS.hold @13:54:11.97 Intake @12.4psia, vacuum from 6.9 to 9.9psi @13:54:11.98 SS.reconfigure :inhaling 0.33ml/s @13:54:12.03 SS.seek 25ml @13:54:14.71 Stroke completed -- waiting for pressure to equalize ... @13:54:14.72 SS.reconfigure :exhale @13:54:14.76 SS.hold @13:54:22.17 Intake.select :close @13:54:22.33 SSV.dial :exhaust @13:54:23.10 Exhaust.select :open @13:54:23.21 SS.seek 0ml @13:54:32.61 Exhaust.select :close @13:54:33.14 SSV.dial CBV @13:54:33.17
CBV.dial SSV @13:54:33.96 Allowing 130s for 25.0ml of remaining 775.0ml @13:54:33.97 Intake.select :open @13:54:36.14 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:54:36.15 SS.reconfigure :inhaling 0.33ml/s @13:54:36.20 SS.seek 25ml @13:55:05.17 Vacuum too high -- waiting up to 40 seconds @13:55:05.18 SS.reconfigure :exhale @13:55:05.23 SS.hold @13:55:18.03 SS.reconfigure :inhaling 0.30ml/s @13:55:18.08 SS.seek 25ml @13:55:39.95 Vacuum too high -- waiting up to 40 seconds @13:55:39.96 SS.reconfigure :exhale @13:55:40.01 SS.hold @13:55:52.90 Intake @14.4psia, vacuum from 8.9 to 11.9psi @13:55:52.91 SS.reconfigure :inhaling 0.28ml/s @13:55:52.96 SS.seek 25ml @13:56:24.63 Vacuum too high -- waiting up to 40 seconds @13:56:24.65 SS.reconfigure :exhale @13:56:24.70 SS.hold @13:56:41.12 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:56:41.50 SS.reconfigure :inhaling 0.25ml/s @13:56:41.62 SS.seek 25ml @13:56:43.98 SS.reconfigure :default @13:56:44.03 SS.hold @13:56:44.19 Intake.select :close @13:56:44.35 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 249.7ml @13:56:44.36 SSV.dial :exhaust @13:56:45.13 Exhaust.select :open @13:56:45.24 SS.reconfigure :exhale @13:56:45.29 SS.seek 0ml @13:56:54.72 Exhaust.select :close @13:56:54.83 Sampled 249.7ml @13:56:54.85 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @13:56:54.92 CSV.dial :waste @13:56:55.05 SSV.dial CBV,avoiding: :exhaust @13:56:55.09
CBV.dial SSV @13:56:55.12 CS.seek 0ml @13:56:55.28 CSV.dial :air @13:56:56.14 CS.seek 10ml @13:56:57.07
CTV.dial :air @13:56:57.84 delay 15 seconds @13:57:12.86 SSV.dial :spit,avoiding: :exhaust @13:57:14.21 CTV.dial CSV,avoiding: :intake @13:57:14.25
SS.seek 0ml @13:57:14.37 SSV.dial CBV,avoiding: :exhaust @13:57:20.51 SSV.dial between CBV and plug @13:57:21.01 CBV.dial between SSV and waste2 @13:57:21.42 CTV.dial CSV,avoiding: :air @13:57:21.44
CSV.dial CTV @13:57:22.06 CS.reconfigure :pressurize @13:57:22.11 CS.seek 0ml @13:57:46.09 BubblePoint: Intake @24.2psia, Outlet @0.4psia @13:57:46.10 CBV.dial SSV @13:57:46.44 SS.reconfigure :default @13:57:46.49 SS.seek 10ml @13:57:51.91 delay 15 seconds @13:58:07.04 Evacuated: Intake @23.8psia, Outlet @23.4psia @13:58:07.05 SSV.dial :spit,avoiding: :exhaust @13:58:08.13 SS.reconfigure :exhale @13:58:08.18 SS.seek 0ml @13:58:13.45 SS.reconfigure :fast @13:58:13.49 SS.seek 5ml @13:58:16.05 SSV.dial CBV,avoiding: :exhaust @13:58:16.08
CBV.dial SSV @13:58:17.44 CTV.dial :air,avoiding: :intake @13:58:18.84 CSV.dial CTV @13:58:18.87
SS.seek 10ml @13:58:19.99 CSV.dial :air @13:58:20.99 CS.reconfigure :fast @13:58:21.05 CS.seek 0ml @13:58:21.36
SSV.dial :spit,avoiding: :exhaust @13:58:22.72 SS.reconfigure :exhale @13:58:22.78 SS.seek 0ml @13:58:23.83 CS.stop @13:58:27.73
SS.reconfigure :fast @13:58:27.78 SS.seek 5ml @13:58:30.60 SSV.dial CBV,avoiding: :exhaust @13:58:30.62
CBV.dial SSV @13:58:33.02 SSV.dial :spit,avoiding: :exhaust @13:58:34.36 SS.reconfigure :default @13:58:34.41 SS.seek 0ml @13:58:37.43 SS.stop @13:58:37.48 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:58:37.49 Intake.select :close @13:58:37.55 CSV.dial :waste @13:58:37.69 SSV.dial CBV,avoiding: :exhaust @13:58:37.71
CBV.dial SSV @13:58:38.34 CS.seek 0ml @13:58:38.42 CSV.dial :air @13:58:39.09
CTV.dial :air @13:58:39.18 CS.seek 10ml @13:58:39.24
delay 15 seconds @13:58:54.26 SSV.dial :spit,avoiding: :exhaust @13:58:55.61 CTV.dial CSV,avoiding: :intake @13:58:55.65
SS.reconfigure :exhale @13:58:55.75 SS.seek 0ml @13:58:55.81 SSV.dial CBV,avoiding: :exhaust @13:59:03.36 SSV.dial between CBV and plug @13:59:03.86 CBV.dial between SSV and waste2 @13:59:04.24 CSV.dial between air and CTV @13:59:04.53 CS.seek 8.074ml @13:59:09.80 CTV.dial CSV,avoiding: :air @13:59:09.82
CSV.dial CTV @13:59:10.17 CS.reconfigure :pressurize @13:59:10.22 CS.seek 0ml @13:59:29.24 BubblePoint: Intake @24.3psia, Outlet @-0.2psia @13:59:29.25 CBV.dial SSV @13:59:29.59 SS.reconfigure :default @13:59:29.64 SS.seek 10ml @13:59:35.23 delay 15 seconds @13:59:50.37 Evacuated: Intake @22.4psia, Outlet @16.3psia @13:59:50.38 SSV.dial :spit,avoiding: :exhaust @13:59:51.44 SS.reconfigure :exhale @13:59:51.92 SS.seek 0ml @13:59:56.84 SS.reconfigure :fast @13:59:56.89 SS.seek 5ml @13:59:59.81 SSV.dial CBV,avoiding: :exhaust @13:59:59.84
CBV.dial SSV @14:00:01.23 CTV.dial :air,avoiding: :intake @14:00:02.62 CSV.dial CTV @14:00:02.66
SS.seek 10ml @14:00:03.78 CSV.dial :air @14:00:04.23 CS.reconfigure :fast @14:00:04.28 CS.seek 0ml @14:00:05.48
SSV.dial :spit,avoiding: :exhaust @14:00:06.85 SS.reconfigure :exhale @14:00:06.90 SS.seek 0ml @14:00:07.10 CS.stop @14:00:11.88
SS.reconfigure :fast @14:00:11.93 SS.seek 5ml @14:00:14.63 SSV.dial CBV,avoiding: :exhaust @14:00:14.65
CBV.dial SSV @14:00:17.04 SSV.dial :spit,avoiding: :exhaust @14:00:18.38 SS.reconfigure :default @14:00:18.43 SS.seek 0ml @14:00:21.60 SS.stop @14:00:21.65 Lysing: Adding 2.0ml AqMeOH @14:00:21.66 CBV.dial between CSV and waste1,avoiding: :DBV @14:00:23.20 CTV.dial CSV @14:00:24.56 CSV.dial CSR @14:00:25.35 Pulling 1ml of CSR.AqMeOH @14:00:25.36 CSR.select :AqMeOH @14:00:25.47 CS.reconfigure :default @14:00:25.52 CS.seek 1ml @14:00:28.88 delay 5 seconds @14:00:33.90 CSR.select :air @14:00:34.07 CS.seek 6ml @14:00:47.60 delay 2 seconds @14:00:49.62 CSV.dial :waste2 @14:00:50.06 CS.reconfigure :fast @14:00:50.11 CS.seek 0ml @14:01:04.73 CSV.dial CSR @14:01:05.18 Pulling 2ml of CSR.AqMeOH @14:01:05.19 CSR.select :AqMeOH @14:01:05.30 CS.reconfigure :default @14:01:05.35 CS.seek 2ml @14:01:11.35 delay 5 seconds @14:01:16.37 CSR.select :air @14:01:16.54 CS.seek 5ml @14:01:25.07 delay 2 seconds @14:01:27.09 CSV.dial CTV @14:01:27.55 CS.seek 1ml @14:01:39.21 Before lysing Collection Puck Heater at 14C/15.4psia @14:01:39.24 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @14:01:39.26 CH.seek 70C,8:00 @14:04:12.22 CH.hold 10:00,70C @14:04:12.42 Begun lysing Collection Puck Heater at 68C/22.4psia @14:14:12.44 Ended lysing Collection Puck Heater at 70C/19.0psia @14:14:12.45 CS.seek 0ml @14:14:15.85 CTV.dial between spare and air,avoiding: :intake @14:14:16.94 CSV.dial :air @14:14:17.39 CS.seek 5ml @14:14:31.06 CSV.dial CTV @14:14:31.51 delay 2 seconds @14:14:33.53 CSV.dial :air @14:14:33.98 delay 2 seconds @14:14:36.00 CSV.dial CTV @14:14:36.45 delay 2 seconds @14:14:38.47 CSV.dial :air @14:14:38.94 delay 2 seconds @14:14:40.96 CS.seek 0ml @14:14:54.71 Puck @27.4psia after final lysis pressurization @14:14:54.72 Recovering Lysate into Collection syringe @14:14:54.73 CSV.dial CBV @14:14:55.73 CBV.dial CSV @14:14:56.12 CS.seek 5ml @14:15:09.78 delay 5 seconds @14:15:14.80 CTV.dial :air @14:15:15.79 CS.seek 7ml @14:15:21.75 delay 5 seconds @14:15:26.77 CSV.dial :air2 @14:15:27.33 CS.seek 10ml @14:15:35.84 CSV.dial between air and CTV,avoiding: CBV @14:15:37.00 Lysing Complete @14:15:37.07 Storing sh1 puck in Collection Clamp to tube 3 @14:15:37.25 SC.reconfigure :tight @14:15:37.30 SC.seek 3 @14:15:41.12 SC.configure :limp @14:15:41.33 Elbow.reconfigure :tight @14:15:41.38 Elbow.seek 3 @14:15:43.08 Forearm.reconfigure :tight @14:15:43.13 Forearm.seek 3 + 800 counts @14:15:44.23 SE.reconfigure :pick @14:15:44.28 SE.seek :beamBreak @14:15:47.01 SE.configure :default @14:15:47.17 Elbow.reconfigure :limp @14:15:47.22 Forearm.reconfigure :loose @14:15:47.26 Forearm.seek 3 @14:15:47.69 Elbow.hold @14:15:48.07 Elbow.reconfigure :tight @14:15:48.17 SE.seek 3.1 Pucks below Top Plate @14:15:49.52 Forearm.force -20 @14:15:49.56 Forearm.seek 3 + 318 counts @14:15:49.85 Forearm.reconfigure :tight @14:15:49.89 Forearm.seek 3 + 800 counts @14:15:50.17 SE.seek 4.1 Pucks below Top Plate @14:15:50.75 Forearm.reconfigure :loose @14:15:50.80 Forearm.seek :retract @14:15:51.78 Elbow.seek CC @14:15:53.38 Elbow.reconfigure :loose @14:15:53.90 Forearm.seek CC @14:15:54.94 Hand.close @14:15:55.68 CC.seek :open @14:15:59.89 Forearm.seek :retract @14:16:00.87 Elbow.reconfigure :tight @14:16:00.92 Elbow.seek 3 @14:16:02.65 Elbow.reconfigure :limp @14:16:02.71 Forearm.seek 3 @14:16:03.85 Elbow.hold @14:16:03.92 Elbow.reconfigure :tight @14:16:03.97 Hand.open @14:16:04.81 Forearm.force -20 @14:16:04.86 Forearm.seek 3 + 319 counts @14:16:05.14 Forearm.reconfigure :tight @14:16:05.19 Forearm.seek 3 + 800 counts @14:16:05.63 SE.seek :down @14:16:06.77
Forearm.reconfigure :loose @14:16:06.82 Forearm.seek :retract @14:16:07.97 SE.stop @14:16:08.00
Tranferring DA @14:16:08.02 Beginning samplePassC @14:16:08.03 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:16:08.22 Elbow.seek FlushPuck::Garage @14:16:10.00 Elbow.reconfigure :loose @14:16:10.11 Forearm.seek :garage @14:16:11.13 Hand.close @14:16:12.05 Forearm.seek :retract @14:16:13.17 Elbow.reconfigure :tight @14:16:13.21 Elbow.seek CC @14:16:16.46 Elbow.reconfigure :loose @14:16:16.56 Forearm.reconfigure :tight @14:16:16.61 Forearm.seek CC @14:16:17.88 CC.seek :closed @14:16:21.89 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.96 @14:16:21.96 Hand.open @14:16:22.74 Forearm.reconfigure :loose @14:16:22.78 Forearm.seek :retract @14:16:23.90 PSV.dial PSR @14:16:25.29 Pulling 1.5ml of PSR.flush @14:16:25.30 PSR.select :flush @14:16:25.42 PS.seek 1.5ml @14:16:41.64 delay 5 seconds @14:16:46.66 PSV.dial :waste1 @14:16:47.13 PS.seek 0ml @14:17:03.36 PSV.dial PSR @14:17:03.83 PSR.select :air @14:17:03.94 PS.seek 2.5ml @14:17:30.27 PRV.dial CBV,avoiding: :ab2 @14:17:30.29
CBV.dial PRV @14:17:32.08 PSV.dial :mixloop @14:17:33.11 CTV.dial :air,avoiding: :intake @14:17:33.18 PTV.dial :PRVmixing @14:17:34.50 PS.seek 0ml @14:18:00.82 PSV.dial :air @14:18:01.30 PS.seek 2.5ml @14:18:27.67 PSV.dial :mixloop @14:18:28.14 PS.seek 0ml @14:18:54.48 PSV.dial :air @14:18:55.40 PS.seek 2.5ml @14:19:21.79 PSV.dial :mixloop @14:19:22.27 PS.seek 0ml @14:19:48.70 Garaging FlushPuck in Collection Clamp @14:19:48.77 Elbow.reconfigure :tight @14:19:48.82 Elbow.seek CC @14:19:48.94 Elbow.reconfigure :loose @14:19:49.05 Forearm.seek CC @14:19:50.00 Hand.close @14:19:50.80 CC.seek :open @14:19:54.89 Forearm.seek :retract @14:19:55.88 Elbow.reconfigure :tight @14:19:55.93 Elbow.seek :garage @14:19:58.78 Elbow.reconfigure :loose @14:19:58.88 Forearm.reconfigure :tight @14:19:58.92 Forearm.seek :garage @14:20:00.03 Hand.open @14:20:00.86 Forearm.reconfigure :loose @14:20:00.91 Forearm.seek :retract @14:20:01.96 PSV.dial :mixloop @14:20:02.03 PTV.dial :PRVmixing @14:20:02.04 PRV.dial CBV @14:20:02.07
CBV.dial PRV @14:20:02.28 CTV.dial :air,avoiding: :intake @14:20:02.36 Loading lyfil puck from tube 4 into Collection Clamp @14:20:02.60 SC.reconfigure :tight @14:20:02.65 SC.seek 4 @14:20:06.32 SC.configure :limp @14:20:06.54 Elbow.reconfigure :tight @14:20:06.59 Elbow.seek 4 @14:20:08.53 Forearm.reconfigure :tight @14:20:08.58 Forearm.seek 4 + 800 counts @14:20:10.02 SE.reconfigure :pick @14:20:10.08 SE.seek :beamBreak @14:20:11.59 SE.configure :default @14:20:11.76 Elbow.seek 4 @14:20:12.00 Forearm.seek 4 + 300 counts @14:20:12.22 Forearm.reconfigure :loose @14:20:12.27 Hand.close @14:20:13.06 SE.seek :down @14:20:13.26
Forearm.seek :retract @14:20:14.19 Elbow.seek CC @14:20:14.31 SE.stop @14:20:15.78
Elbow.reconfigure :loose @14:20:15.88 Forearm.reconfigure :tight @14:20:15.93 Forearm.seek CC @14:20:17.21 CC.seek :closed @14:20:21.19 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.01 @14:20:21.20 Hand.open @14:20:22.00 Forearm.reconfigure :loose @14:20:22.05 Forearm.seek :retract @14:20:23.04 CTV.dial CSV,avoiding: :intake @14:20:24.41 CSV.dial CTV @14:20:24.73 PS.seek 2.5ml @14:20:51.17 CS.seek 5ml @14:21:05.27 delay 8 seconds @14:21:13.30 PSV.dial :air @14:21:13.76 delay 2 seconds @14:21:15.78 PSV.dial :waste2 @14:21:16.53 PS.seek 0ml @14:21:42.85 CBV.dial between PRV and waste1 @14:21:43.20 CSV.dial :air @14:21:43.66 CS.seek 10ml @14:21:57.27 CSV.dial CTV @14:21:57.73 CS.seek 0ml @14:22:24.15 CBV.dial :waste1 @14:22:24.46 delay 5 seconds @14:22:29.48 CTV.dial :air,avoiding: :intake @14:22:30.93 Storing lyfil puck in Collection Clamp to tube 3 @14:22:31.11 SC.reconfigure :tight @14:22:31.15 SC.seek 3 @14:22:34.83 SC.configure :limp @14:22:35.05 Elbow.reconfigure :tight @14:22:35.10 Elbow.seek 3 @14:22:36.79 Forearm.reconfigure :tight @14:22:36.84 Forearm.seek 3 + 800 counts @14:22:38.05 SE.reconfigure :pick @14:22:38.10 SE.seek :beamBreak @14:22:40.73 SE.configure :default @14:22:40.90 Elbow.reconfigure :limp @14:22:40.95 Forearm.reconfigure :loose @14:22:40.99 Forearm.seek 3 @14:22:41.41 Elbow.hold @14:22:41.47 Elbow.reconfigure :tight @14:22:41.57 SE.seek 4.1 Pucks below Top Plate @14:22:42.01 Forearm.force -20 @14:22:42.06 Forearm.seek 3 + 315 counts @14:22:42.89 Forearm.reconfigure :tight @14:22:42.94 Forearm.seek 3 + 800 counts @14:22:43.21 SE.seek 5.1 Pucks below Top Plate @14:22:43.78 Forearm.reconfigure :loose @14:22:43.83 Forearm.seek :retract @14:22:44.75 Elbow.seek CC @14:22:46.39 Elbow.reconfigure :loose @14:22:46.49 Forearm.seek CC @14:22:47.50 Hand.close @14:22:48.30 CC.seek :open @14:22:52.51 Forearm.seek :retract @14:22:53.55 Elbow.reconfigure :tight @14:22:53.60 Elbow.seek 3 @14:22:55.32 Elbow.reconfigure :limp @14:22:55.38 Forearm.seek 3 @14:22:56.55 Elbow.hold @14:22:56.62 Elbow.reconfigure :tight @14:22:56.67 Hand.open @14:22:57.55 Forearm.force -20 @14:22:57.60 Forearm.seek 3 + 319 counts @14:22:57.89 Forearm.reconfigure :tight @14:22:57.94 Forearm.seek 3 + 800 counts @14:22:58.29 SE.seek :down @14:22:59.43
Forearm.reconfigure :loose @14:22:59.48 Forearm.seek :retract @14:23:00.52 SE.stop @14:23:01.00
PSV.dial :air @14:23:01.75 PS.seek 2.5ml @14:23:28.13 delay 1 second @14:23:29.15 PSV.dial :mixloop @14:23:29.61 delay 1 second @14:23:30.62 PSV.dial :air @14:23:31.09 delay 1 second @14:23:32.11 PSV.dial :mixloop @14:23:32.57 delay 1 second @14:23:33.59 PSV.dial :air @14:23:34.06 delay 1 second @14:23:35.08 PS.seek 0ml @14:24:01.45 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:24:01.64 Elbow.seek FlushPuck::Garage @14:24:03.42 Elbow.reconfigure :loose @14:24:03.52 Forearm.seek :garage @14:24:04.56 Hand.close @14:24:05.42 Forearm.seek :retract @14:24:06.42 Elbow.reconfigure :tight @14:24:06.48 Elbow.seek PC @14:24:07.79 Elbow.reconfigure :loose @14:24:07.90 Forearm.reconfigure :tight @14:24:07.94 Forearm.seek PC @14:24:09.30 PC.seek :closed @14:24:13.82 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.53 @14:24:13.88 Hand.open @14:24:14.74 Forearm.reconfigure :loose @14:24:14.78 Forearm.seek :retract @14:24:15.78 PRV.dial :air @14:24:16.24 PSV.dial :mixloop @14:24:16.79 PS.seek 0.3ml @14:24:20.73 PSV.dial PSR @14:24:21.78 Pulling 0.4ml of PSR.air PSR.select :air @14:24:21.81 PS.seek 0.7ml @14:24:26.80 delay 3 seconds @14:24:29.82 PSR.select :air @14:24:29.84 PS.seek 2.5ml @14:24:49.10 delay 3 seconds @14:24:52.12 PSV.dial :mixloop @14:24:53.17 PTV.dial :mixing @14:24:53.92 PBV.dial :waste2 @14:24:54.91 PS.seek 0ml @14:25:21.23 Garaging FlushPuck in Processing Clamp @14:25:21.30 Elbow.reconfigure :tight @14:25:21.35 Elbow.seek PC @14:25:21.51 Elbow.reconfigure :loose @14:25:21.61 Forearm.seek PC @14:25:22.69 Hand.close @14:25:23.42 PC.seek :open @14:25:27.91 Forearm.seek :retract @14:25:28.91 Elbow.reconfigure :tight @14:25:28.95 Elbow.seek :garage @14:25:30.41 Elbow.reconfigure :loose @14:25:30.52 Forearm.reconfigure :tight @14:25:30.56 Forearm.seek :garage @14:25:31.62 Hand.open @14:25:32.48 Forearm.reconfigure :loose @14:25:32.53 Forearm.seek :retract @14:25:33.60 Loading da puck from tube 4 into Processing Clamp @14:25:33.84 SC.reconfigure :tight @14:25:33.89 SC.seek 4 @14:25:37.52 SC.configure :limp @14:25:37.74 Elbow.reconfigure :tight @14:25:37.79 Elbow.seek 4 @14:25:40.09 Forearm.reconfigure :tight @14:25:40.14 Forearm.seek 4 + 800 counts @14:25:41.17 SE.reconfigure :pick @14:25:41.23 SE.seek :beamBreak @14:25:42.76 SE.configure :default @14:25:42.93 Elbow.seek 4 @14:25:43.11 Forearm.seek 4 + 300 counts @14:25:43.39 Forearm.reconfigure :loose @14:25:43.44 Hand.close @14:25:44.18 SE.seek :down @14:25:44.39
Forearm.seek :retract @14:25:45.43 Elbow.seek PC @14:25:45.52 SE.stop @14:25:48.00
Elbow.reconfigure :loose @14:25:48.11 Forearm.reconfigure :tight @14:25:48.16 Forearm.seek PC @14:25:49.53 PC.seek :closed @14:25:53.96 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.53 @14:25:53.97 Hand.open @14:25:54.73 Forearm.reconfigure :loose @14:25:54.78 Forearm.seek :retract @14:25:55.80 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @14:25:55.82 PH.seek 28C,5:00 @14:26:41.70 PH.hold 2:00:00,28C @14:26:41.75 delay 1:30 @14:28:11.80 PSR.select :air @14:28:11.81 PSV.dial PSR @14:28:12.87 Pulling 0.5ml of PSR.wash @14:28:12.88 PSR.select :wash @14:28:13.00 PS.seek 0.5ml @14:28:18.97 delay 5 seconds @14:28:23.99 PSR.select :air @14:28:24.12 PS.seek 2ml @14:28:40.25 PTV.dial :puck @14:28:40.99 PSV.dial PTV @14:28:41.76 PBV.dial :waste1 @14:28:43.19 PS.seek 1.5ml @14:28:49.10 delay 3 seconds @14:28:52.12 PBV.dial between PSV and waste1 @14:28:52.95 PS.seek 0ml @14:29:09.19 delay 5:00 @14:34:09.22 recoverPSRreagent08 @14:34:09.23 PTV.dial :puck @14:34:09.30 PBV.dial :waste1 @14:34:09.69 delay 30 seconds @14:34:39.72 PBV.dial between PSV and waste1 @14:34:40.10 PSR.select :air @14:34:40.11 PSV.dial PSR @14:34:40.87 PS.reconfigure :fast @14:34:40.92 PS.seek 2.5ml @14:35:04.47 PSV.dial PTV @14:35:05.22 delay 5 seconds @14:35:10.24 PSV.dial PSR @14:35:11.11 delay 5 seconds @14:35:16.13 PSV.dial PTV @14:35:16.86 delay 5 seconds @14:35:21.88 PSV.dial :air @14:35:22.66 PS.seek 0ml @14:35:45.75 PTV.dial :PRVmixing,via: PRV @14:35:47.08 PSV.dial :mixloop @14:35:47.55 PRV.dial :ab1 @14:35:48.30 PS.reconfigure :slow @14:35:48.35 PS.seek 0.15ml @14:35:51.91 delay 2 seconds @14:35:53.93 CBV.dial PRV @14:35:53.95
PRV.dial CBV @14:35:54.54 PS.seek 0.34ml @14:35:58.91 delay 2 seconds @14:36:00.92 CBV.dial between PRV and PSV @14:36:01.25 PRV.dial :air @14:36:01.71 PS.seek 0.9ml @14:36:13.69 PRV.dial :waste2 @14:36:14.45 PS.seek 0.5ml @14:36:23.16 addPMLReagent08 @14:36:23.17 PSV.dial :air @14:36:23.63 PS.reconfigure :default @14:36:23.68 PS.seek 2ml @14:36:39.81 PSV.dial :PML @14:36:40.28 PTV.dial :mixing @14:36:41.02 PBV.dial :waste1 @14:36:41.45 PS.seek 1.85ml @14:36:43.84 PBV.dial between PSV and waste1 @14:36:44.23 PS.seek 0ml @14:37:03.94 delay 2 seconds @14:37:05.96 Beginning samplePassC @14:37:05.97 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:37:06.68 Elbow.reconfigure :tight @14:37:06.74 Elbow.seek FlushPuck::Garage @14:37:08.03 Elbow.reconfigure :loose @14:37:08.13 Forearm.seek :garage @14:37:09.18 Hand.close @14:37:10.03 Forearm.seek :retract @14:37:11.02 Elbow.reconfigure :tight @14:37:11.07 Elbow.seek CC @14:37:13.91 Elbow.reconfigure :loose @14:37:14.01 Forearm.reconfigure :tight @14:37:14.06 Forearm.seek CC @14:37:15.38 CC.seek :closed @14:37:19.39 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.06 @14:37:19.45 Hand.open @14:37:20.23 Forearm.reconfigure :loose @14:37:20.28 Forearm.seek :retract @14:37:21.24 PSV.dial PSR @14:37:22.41 Pulling 1.5ml of PSR.flush @14:37:22.42 PSR.select :flush @14:37:22.54 PS.seek 1.5ml @14:37:38.66 delay 5 seconds @14:37:43.68 PSV.dial :waste1 @14:37:44.29 PS.seek 0ml @14:38:00.37 PSV.dial PSR @14:38:00.83 PSR.select :air @14:38:00.94 PS.seek 2.5ml @14:38:27.28 PRV.dial CBV,avoiding: :ab2 @14:38:27.31
CBV.dial PRV @14:38:28.97 PSV.dial :mixloop @14:38:30.47 CTV.dial :air,avoiding: :intake @14:38:30.56 PTV.dial :PRVmixing @14:38:31.32 PS.seek 0ml @14:38:57.69 PSV.dial :air @14:38:58.16 PS.seek 2.5ml @14:39:24.56 PSV.dial :mixloop @14:39:25.04 PS.seek 0ml @14:39:51.37 PSV.dial :air @14:39:51.85 PS.seek 2.5ml @14:40:18.19 PSV.dial :mixloop @14:40:18.66 PS.seek 0ml @14:40:45.00 Garaging FlushPuck in Collection Clamp @14:40:45.07 Elbow.reconfigure :tight @14:40:45.12 Elbow.seek CC @14:40:45.24 Elbow.reconfigure :loose @14:40:45.35 Forearm.seek CC @14:40:46.30 Hand.close @14:40:47.03 CC.seek :open @14:40:51.16 Forearm.seek :retract @14:40:52.13 Elbow.reconfigure :tight @14:40:52.17 Elbow.seek :garage @14:40:55.06 Elbow.reconfigure :loose @14:40:55.16 Forearm.reconfigure :tight @14:40:55.21 Forearm.seek :garage @14:40:56.27 Hand.open @14:40:57.09 Forearm.reconfigure :loose @14:40:57.14 Forearm.seek :retract @14:40:58.13 delayUntil "14:45:05.94" @14:40:58.16 Whole Cell Archive sampling 1000ml @14:40:58.17 Loading wcr puck from tube 4 into Collection Clamp @14:40:58.41 SC.reconfigure :tight @14:40:58.47 SC.seek 4 @14:40:58.53 SC.configure :limp @14:40:59.20 Elbow.reconfigure :tight @14:40:59.25 Elbow.seek 4 @14:41:01.14 Forearm.reconfigure :tight @14:41:01.20 Forearm.seek 4 + 800 counts @14:41:02.23 SE.reconfigure :pick @14:41:02.29 SE.seek :beamBreak @14:41:03.97 SE.configure :default @14:41:04.14 Elbow.seek 4 @14:41:04.33 Forearm.seek 4 + 300 counts @14:41:04.51 Forearm.reconfigure :loose @14:41:04.56 Hand.close @14:41:05.30 SE.seek :down @14:41:05.51 Forearm.seek :retract @14:41:06.67 Elbow.seek CC @14:41:06.74 SE.stop @14:41:08.21 Elbow.reconfigure :loose @14:41:08.32 Forearm.reconfigure :tight @14:41:08.38 Forearm.seek CC @14:41:09.76 CC.seek :closed @14:41:13.73 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.96 @14:41:13.75 Hand.open @14:41:14.97 Forearm.reconfigure :loose @14:41:15.02 Forearm.seek :retract @14:41:16.02 collectSample 1000ml w/bubble point of 35psi @14:41:16.03 Priming 7ml @14:41:16.04 Intake.select :close @14:41:16.05 Exhaust.select :close @14:41:16.07 SS.hold @14:41:16.28 Calibrating pressure sensors @14:41:16.29 CTV.dial :air @14:41:16.37 SSV.dial :spit,avoiding: :exhaust @14:41:16.46 SS.reconfigure :fast @14:41:16.51 SS.seek 5ml @14:41:19.20 SSV.dial CBV @14:41:19.23 CBV.dial SSV @14:41:20.65 delay 3.8 seconds @14:41:24.60 Sensor limits: Intake max 99.3psia; Outlet max 108.6psia @14:41:24.61 SSV.dial :spit @14:41:25.97 SS.seek 0ml @14:41:28.75 SSV.dial CBV @14:41:30.11 CTV.dial :intake @14:41:30.90 SSV.dial CBV @14:41:30.93 CBV.dial SSV @14:41:31.08 Allowing 40s for 7.0ml of remaining 7.0ml @14:41:31.09 Intake.select :open @14:41:33.27 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:41:33.29 SS.reconfigure :inhaling 2.17ml/s @14:41:33.34 SS.seek 7ml @14:41:40.43 Stroke completed -- waiting for pressure to equalize ... @14:41:40.44 SS.reconfigure :exhale @14:41:40.49 SS.hold @14:41:48.43 Intake.select :close @14:41:48.59 SSV.dial :exhaust @14:41:49.39 Exhaust.select :open @14:41:49.50 SS.seek 0ml @14:41:53.74 Exhaust.select :close @14:41:53.86 Sampling 1000.0ml @14:41:53.87 Intake.select :close @14:41:53.88 Exhaust.select :close @14:41:53.90 SS.reconfigure :default @14:41:53.94 SS.hold @14:41:54.15 CTV.dial :intake @14:41:54.23 SSV.dial CBV @14:41:54.27 CBV.dial SSV @14:41:55.07 Allowing 130s for 25.0ml of remaining 1000.0ml @14:41:55.09 Intake.select :open @14:41:57.26 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:41:57.28 SS.reconfigure :inhaling 2.17ml/s @14:41:57.33 SS.seek 25ml @14:42:05.99 Vacuum too high -- waiting up to 40 seconds @14:42:06.00 SS.reconfigure :exhale @14:42:06.06 SS.hold @14:42:06.79 Intake @9.9psia, vacuum from 4.4 to 7.4psi @14:42:06.80 SS.reconfigure :inhaling 1.95ml/s @14:42:06.85 SS.seek 25ml @14:42:07.36 Vacuum too high -- waiting up to 40 seconds @14:42:07.38 SS.reconfigure :exhale @14:42:07.44 SS.hold @14:42:15.21 Intake @10.9psia, vacuum from 5.4 to 8.4psi @14:42:15.23 SS.reconfigure :inhaling 1.77ml/s @14:42:15.28 SS.seek 25ml @14:42:21.58 Vacuum too high -- waiting up to 40 seconds @14:42:21.60 SS.reconfigure :exhale @14:42:21.65 SS.hold @14:42:24.90 Intake @9.9psia, vacuum from 4.4 to 7.4psi @14:42:24.92 SS.reconfigure :inhaling 1.59ml/s @14:42:24.97 SS.seek 25ml @14:42:26.48 Stroke completed -- waiting for pressure to equalize ... @14:42:26.49 SS.reconfigure :exhale @14:42:26.54 SS.hold @14:42:31.07 Intake.select :close @14:42:31.23 SSV.dial :exhaust @14:42:32.03 Exhaust.select :open @14:42:32.14 SS.seek 0ml @14:42:41.40 Exhaust.select :close @14:42:41.51 SSV.dial CBV @14:42:41.55 CBV.dial SSV @14:42:42.32 Allowing 130s for 25.0ml of remaining 975.0ml @14:42:42.34 Intake.select :open @14:42:44.51 Intake @13.6psia, vacuum from 8.1 to 11.1psi @14:42:44.53 SS.reconfigure :inhaling 1.59ml/s @14:42:44.58 SS.seek 25ml @14:42:50.49 Vacuum too high -- waiting up to 40 seconds @14:42:50.50 SS.reconfigure :exhale @14:42:50.56 SS.hold @14:42:51.09 Intake @9.7psia, vacuum from 4.2 to 7.2psi @14:42:51.11 SS.reconfigure :inhaling 1.43ml/s @14:42:51.16 SS.seek 25ml @14:42:53.61 Vacuum too high -- waiting up to 40 seconds @14:42:53.63 SS.reconfigure :exhale @14:42:53.68 SS.hold @14:42:57.85 Intake @11.7psia, vacuum from 6.2 to 9.2psi @14:42:57.87 SS.reconfigure :inhaling 1.29ml/s @14:42:57.92 SS.seek 25ml @14:43:04.30 Vacuum too high -- waiting up to 40 seconds @14:43:04.31 SS.reconfigure :exhale @14:43:04.37 SS.hold @14:43:07.03 Intake @10.3psia, vacuum from 4.8 to 7.8psi @14:43:07.05 SS.reconfigure :inhaling 1.16ml/s @14:43:07.10 SS.seek 25ml @14:43:12.55 Vacuum too high -- waiting up to 40 seconds @14:43:12.56 SS.reconfigure :exhale @14:43:12.62 SS.hold @14:43:16.94 Intake @13.7psia, vacuum from 8.2 to 11.2psi @14:43:16.96 SS.reconfigure :inhaling 1.04ml/s @14:43:17.01 SS.seek 25ml @14:43:20.43 Stroke completed -- waiting for pressure to equalize ... @14:43:20.44 SS.reconfigure :exhale @14:43:20.49 SS.hold @14:43:23.96 Intake.select :close @14:43:24.13 SSV.dial :exhaust @14:43:24.92 Exhaust.select :open @14:43:25.04 SS.seek 0ml @14:43:34.20 Exhaust.select :close @14:43:34.31 SSV.dial CBV @14:43:34.33 CBV.dial SSV @14:43:35.15 Allowing 130s for 25.0ml of remaining 950.0ml @14:43:35.16 Intake.select :open @14:43:37.33 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:43:37.35 SS.reconfigure :inhaling 1.04ml/s @14:43:37.40 SS.seek 25ml @14:43:48.83 Vacuum too high -- waiting up to 40 seconds @14:43:48.84 SS.reconfigure :exhale @14:43:48.90 SS.hold @14:43:49.95 Intake @11.5psia, vacuum from 6.0 to 9.0psi @14:43:49.97 SS.reconfigure :inhaling 0.94ml/s @14:43:50.02 SS.seek 25ml @14:43:56.70 Vacuum too high -- waiting up to 40 seconds @14:43:56.72 SS.reconfigure :exhale @14:43:56.77 SS.hold @14:44:00.32 Intake @11.8psia, vacuum from 6.3 to 9.3psi @14:44:00.34 SS.reconfigure :inhaling 0.85ml/s @14:44:00.39 SS.seek 25ml @14:44:11.63 Stroke completed -- waiting for pressure to equalize ... @14:44:11.64 SS.reconfigure :exhale @14:44:11.69 SS.hold @14:44:16.73 Intake.select :close @14:44:16.89 SSV.dial :exhaust @14:44:17.66 Exhaust.select :open @14:44:17.78 SS.seek 0ml @14:44:27.09 Exhaust.select :close @14:44:27.20 SSV.dial CBV @14:44:27.23 CBV.dial SSV @14:44:28.04 Allowing 130s for 25.0ml of remaining 925.0ml @14:44:28.05 Intake.select :open @14:44:30.22 Intake @13.9psia, vacuum from 8.4 to 11.4psi @14:44:30.24 SS.reconfigure :inhaling 0.85ml/s @14:44:30.29 SS.seek 25ml @14:44:47.67 Vacuum too high -- waiting up to 40 seconds @14:44:47.69 SS.reconfigure :exhale @14:44:47.74 SS.hold @14:44:48.63 Intake @11.4psia, vacuum from 5.9 to 8.9psi @14:44:48.65 SS.reconfigure :inhaling 0.77ml/s @14:44:48.70 SS.seek 25ml @14:44:58.23 Vacuum too high -- waiting up to 40 seconds @14:44:58.25 SS.reconfigure :exhale @14:44:58.31 SS.hold @14:45:00.89 Intake @12.0psia, vacuum from 6.5 to 9.5psi @14:45:00.91 SS.reconfigure :inhaling 0.70ml/s @14:45:00.97 SS.seek 25ml @14:45:05.95 recoverPRVreagent08 @14:45:05.97 PTV.dial :mixing @14:45:06.71 PBV.dial :waste1 @14:45:07.10 delay 30 seconds @14:45:07.59 Stroke completed -- waiting for pressure to equalize ... @14:45:07.60 SS.reconfigure :exhale @14:45:07.65 SS.hold @14:45:10.53 Intake.select :close @14:45:10.68 SSV.dial :exhaust @14:45:11.47 Exhaust.select :open @14:45:11.59 SS.seek 0ml @14:45:20.85 Exhaust.select :close @14:45:20.96 SSV.dial CBV @14:45:20.99 CBV.dial SSV @14:45:22.24 Allowing 130s for 25.0ml of remaining 900.0ml @14:45:22.26 Intake.select :open @14:45:24.43 Intake @14.4psia, vacuum from 8.9 to 11.9psi @14:45:24.45 SS.reconfigure :inhaling 0.70ml/s @14:45:24.50 SS.seek 25ml @14:45:37.13 PBV.dial between PSV and waste1 @14:45:37.53 PSV.dial :air @14:45:38.00 PS.reconfigure :fast @14:45:38.05 PS.seek 2.5ml @14:45:49.02 Vacuum too high -- waiting up to 40 seconds @14:45:49.03 SS.reconfigure :exhale @14:45:49.09 SS.hold @14:45:50.04 Intake @13.2psia, vacuum from 7.7 to 10.7psi @14:45:50.06 SS.reconfigure :inhaling 0.62ml/s @14:45:50.11 SS.seek 25ml @14:45:58.67 Vacuum too high -- waiting up to 40 seconds @14:45:58.69 SS.reconfigure :exhale @14:45:58.74 SS.hold @14:46:01.79 PSV.dial :mixloop @14:46:02.29 delay 2 seconds @14:46:02.44 Intake @12.2psia, vacuum from 6.7 to 9.7psi @14:46:02.46 SS.reconfigure :inhaling 0.56ml/s @14:46:02.51 SS.seek 25ml @14:46:04.32 PSV.dial :air @14:46:04.79 delay 2 seconds @14:46:06.81 PSV.dial :mixloop @14:46:07.28 delay 2 seconds @14:46:09.22 Stroke completed -- waiting for pressure to equalize ... @14:46:09.24 SS.reconfigure :exhale @14:46:09.28 SS.hold @14:46:09.36 PSV.dial :air @14:46:10.36 PS.seek 0ml @14:46:14.48 Intake.select :close @14:46:14.64 SSV.dial :exhaust @14:46:15.43 Exhaust.select :open @14:46:15.54 SS.seek 0ml @14:46:24.93 Exhaust.select :close @14:46:25.04 SSV.dial CBV @14:46:25.08 CBV.dial SSV @14:46:25.87 Allowing 130s for 25.0ml of remaining 875.0ml @14:46:25.88 Intake.select :open @14:46:28.05 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:46:28.06 SS.reconfigure :inhaling 0.56ml/s @14:46:28.11 SS.seek 25ml @14:46:34.03 washCycleDA #1 of 2 @14:46:34.04 PSR.select :air @14:46:34.05 PSV.dial PSR @14:46:35.40 Pulling 0.5ml of PSR.wash @14:46:35.41 PSR.select :wash @14:46:35.53 PS.reconfigure :default @14:46:35.58 PS.seek 0.5ml @14:46:41.49 delay 5 seconds @14:46:45.42 Vacuum too high -- waiting up to 40 seconds @14:46:45.44 SS.reconfigure :exhale @14:46:45.49 SS.hold @14:46:46.51 PSR.select :air @14:46:46.63 PS.seek 2ml @14:46:50.68 Intake @13.6psia, vacuum from 8.1 to 11.1psi @14:46:50.70 SS.reconfigure :inhaling 0.51ml/s @14:46:50.76 SS.seek 25ml @14:47:02.81 PTV.dial :puck @14:47:03.56 PSV.dial PTV @14:47:03.65 Vacuum too high -- waiting up to 40 seconds @14:47:03.66 SS.reconfigure :exhale @14:47:03.72 SS.hold @14:47:04.39 PBV.dial :waste1 @14:47:04.83 PS.seek 1.5ml @14:47:10.36 SS.reconfigure :inhaling 0.46ml/s @14:47:10.41 SS.seek 25ml @14:47:10.81 delay 3 seconds @14:47:13.82 PBV.dial between PSV and waste1 @14:47:14.69 PS.seek 0ml @14:47:30.81 delay 2:00 @14:47:30.96 Stroke completed -- waiting for pressure to equalize ... @14:47:30.97 SS.reconfigure :exhale @14:47:31.02 SS.hold @14:47:49.43 Intake.select :close @14:47:49.59 SSV.dial :exhaust @14:47:50.36 Exhaust.select :open @14:47:50.48 SS.seek 0ml @14:47:59.78 Exhaust.select :close @14:48:00.30 SSV.dial CBV @14:48:00.33 CBV.dial SSV @14:48:01.14 Allowing 130s for 25.0ml of remaining 850.0ml @14:48:01.16 Intake.select :open @14:48:03.33 Intake @14.4psia, vacuum from 8.9 to 11.9psi @14:48:03.35 SS.reconfigure :inhaling 0.46ml/s @14:48:03.40 SS.seek 25ml @14:48:13.79 Vacuum too high -- waiting up to 40 seconds @14:48:13.81 SS.reconfigure :exhale @14:48:13.87 SS.hold @14:48:26.69 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:48:26.71 SS.reconfigure :inhaling 0.42ml/s @14:48:26.76 SS.seek 25ml @14:48:36.70 Vacuum too high -- waiting up to 40 seconds @14:48:36.71 SS.reconfigure :exhale @14:48:36.77 SS.hold @14:48:53.68 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:48:53.70 SS.reconfigure :inhaling 0.38ml/s @14:48:53.75 SS.seek 25ml @14:49:04.89 Vacuum too high -- waiting up to 40 seconds @14:49:04.90 SS.reconfigure :exhale @14:49:04.96 SS.hold @14:49:23.65 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:49:23.67 SS.reconfigure :inhaling 0.33ml/s @14:49:23.72 SS.seek 25ml @14:49:30.82 recoverPSRreagent08 @14:49:30.84 PTV.dial :puck @14:49:30.91 PBV.dial :waste1 @14:49:31.31 delay 30 seconds @14:49:38.32 Vacuum too high -- waiting up to 40 seconds @14:49:38.34 SS.reconfigure :exhale @14:49:38.40 SS.hold @14:50:01.18 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:50:01.20 SS.reconfigure :inhaling 0.30ml/s @14:50:01.24 SS.seek 25ml @14:50:01.32 PBV.dial between PSV and waste1 @14:50:01.71 PSR.select :air @14:50:01.73 PSV.dial PSR @14:50:02.50 PS.reconfigure :fast @14:50:02.55 PS.seek 2.5ml @14:50:11.18 SS.reconfigure :default @14:50:11.24 SS.hold @14:50:11.36 Intake.select :close @14:50:11.52 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 170.1ml @14:50:11.53 SSV.dial :exhaust @14:50:12.31 Exhaust.select :open @14:50:12.43 SS.reconfigure :exhale @14:50:12.48 SS.seek 0ml @14:50:20.59 Exhaust.select :close @14:50:20.71 Sampled 170.1ml @14:50:20.73 Drying puck before fixing for 4:00 @14:50:20.74 CBV.dial :waste2b @14:50:21.50 delay 1:00 @14:50:26.30 PSV.dial PTV @14:50:27.55 delay 5 seconds @14:50:32.58 PSV.dial PSR @14:50:33.32 delay 5 seconds @14:50:38.34 PSV.dial PTV @14:50:39.10 delay 5 seconds @14:50:44.12 PSV.dial :air @14:50:44.89 PS.seek 0ml @14:51:08.15 washCycleDA #2 of 2 @14:51:08.16 PSR.select :air @14:51:08.18 PSV.dial PSR @14:51:09.51 Pulling 0.5ml of PSR.wash @14:51:09.52 PSR.select :wash @14:51:09.64 PS.reconfigure :default @14:51:09.69 PS.seek 0.5ml @14:51:15.71 delay 5 seconds @14:51:20.73 PSR.select :air @14:51:20.85 PS.seek 2ml @14:51:21.52 CSV.dial :air @14:51:21.97 CS.reconfigure :fast @14:51:22.03 CS.seek 10ml @14:51:36.96 PTV.dial :puck @14:51:37.04 PSV.dial PTV @14:51:37.79 PBV.dial :waste1 @14:51:38.24 PS.seek 1.5ml @14:51:44.18 delay 3 seconds @14:51:45.97 Pumping wcr puck from tube 4 up to 35psi... @14:51:45.99 CBV.dial SSV @14:51:47.19 PBV.dial between PSV and waste1 @14:51:47.26 CBV.dial between CSV and waste2b @14:51:47.59 PS.seek 0ml @14:51:48.25 CSV.dial between air and CTV @14:51:48.57 CS.seek 7.091ml @14:51:56.13 CTV.dial CSV,avoiding: :air @14:51:56.16 CSV.dial CTV @14:51:56.99 CS.reconfigure :pressurize @14:51:57.04 CS.seek 0ml @14:52:03.71 delay 2:00 @14:52:15.02 CBV.dial :waste2b @14:52:15.38 CS.reconfigure :default @14:52:15.43 CS.seek 0ml @14:52:16.84 delay 1:00 @14:53:16.87 CTV.dial :air,avoiding: :intake @14:53:18.25 Fix #1 of 1 (with RNAlater) for 4:00 @14:53:18.27 CSV.dial CSR @14:53:18.83 Pulling 2ml of CSR.RNAlater @14:53:18.84 CSR.select :RNAlater @14:53:18.95 CS.seek 2ml @14:53:25.38 delay 5 seconds @14:53:30.41 CSR.select :air @14:53:30.53 CS.reconfigure :fast @14:53:30.59 CS.seek 10ml @14:53:49.87 Pumping wcr puck from tube 4 up to 35psi... @14:53:49.89 CBV.dial SSV @14:53:51.15 CBV.dial between CSV and waste2b @14:53:52.12 CSV.dial between air and CTV @14:53:52.71 CS.seek 6.923ml @14:54:00.60 CTV.dial CSV,avoiding: :air @14:54:00.63 CSV.dial CTV @14:54:02.05 CS.reconfigure :pressurize @14:54:02.10 CS.seek 0ml @14:54:03.73 recoverPSRreagent08 @14:54:03.74 PTV.dial :puck @14:54:03.82 PBV.dial :waste1 @14:54:04.21 delay 30 seconds @14:54:17.24 delay 4:00 @14:54:34.23 PBV.dial between PSV and waste1 @14:54:34.63 PSR.select :air @14:54:34.65 PSV.dial PSR @14:54:35.40 PS.reconfigure :fast @14:54:35.45 PS.seek 2.5ml @14:54:58.99 PSV.dial PTV @14:54:59.74 delay 5 seconds @14:55:04.76 PSV.dial PSR @14:55:05.52 delay 5 seconds @14:55:10.54 PSV.dial PTV @14:55:11.30 delay 5 seconds @14:55:16.32 PSV.dial :air @14:55:17.09 PS.seek 0ml @14:55:40.43 getPMLReagent08 @14:55:40.44 PRV.dial :air @14:55:40.91 delay 5 seconds @14:55:45.93 PTV.dial :PRVmixing @14:55:47.24 delay 5 seconds @14:55:52.26 PRV.dial :ab2 @14:55:52.71 PTV.dial :PRVmixing @14:55:52.78 PSV.dial :mixloop @14:55:53.26 PS.reconfigure :default @14:55:53.31 PS.seek 0.3ml @14:55:57.17 delay 2 seconds @14:55:59.19 PRV.dial :air @14:55:59.72 PS.seek 0.55ml @14:56:03.19 addPMLReagent08 @14:56:03.20 PSV.dial :air @14:56:03.66 PS.seek 2ml @14:56:19.33 PSV.dial :PML @14:56:19.80 PTV.dial :mixing @14:56:20.55 PBV.dial :waste1 @14:56:21.47 PS.seek 1.85ml @14:56:23.80 PBV.dial between PSV and waste1 @14:56:24.18 PS.seek 0ml @14:56:43.89 delay 2 seconds @14:56:45.91 delay 8:00 @14:58:17.26 CBV.dial :waste2b @14:58:17.61 CS.reconfigure :default @14:58:17.66 CS.seek 0ml @14:58:21.62 delay 1:00 @14:59:21.65 CTV.dial :air,avoiding: :intake @15:04:45.93 recoverPRVreagent08 @15:04:45.94 PTV.dial :mixing @15:04:46.01 PBV.dial :waste1 @15:04:46.40 delay 30 seconds @15:05:16.43 PBV.dial between PSV and waste1 @15:05:16.82 PSV.dial :air @15:05:17.30 PS.reconfigure :fast @15:05:17.35 PS.seek 2.5ml @15:05:40.92 PSV.dial :mixloop @15:05:41.39 delay 2 seconds @15:05:43.41 PSV.dial :air @15:05:43.87 delay 2 seconds @15:05:45.89 PSV.dial :mixloop @15:05:46.36 delay 2 seconds @15:05:48.37 PSV.dial :air @15:05:48.84 PS.seek 0ml @15:06:12.14 washCycleDA #1 of 3 @15:06:12.15 PSR.select :air @15:06:12.16 PSV.dial PSR @15:06:13.51 Pulling 0.5ml of PSR.wash @15:06:13.52 PSR.select :wash @15:06:13.64 PS.reconfigure :default @15:06:13.69 PS.seek 0.5ml @15:06:19.67 delay 5 seconds @15:06:24.69 PSR.select :air @15:06:24.81 PS.seek 2ml @15:06:40.91 PTV.dial :puck @15:06:41.67 PSV.dial PTV @15:06:42.40 PBV.dial :waste1 @15:06:42.85 PS.seek 1.5ml @15:06:48.85 delay 3 seconds @15:06:51.87 PBV.dial between PSV and waste1 @15:06:52.27 PS.seek 0ml @15:07:08.39 delay 2:00 @15:09:08.41 recoverPSRreagent08 @15:09:08.42 PTV.dial :puck @15:09:08.49 PBV.dial :waste1 @15:09:08.89 delay 30 seconds @15:09:38.91 PBV.dial between PSV and waste1 @15:09:39.30 PSR.select :air @15:09:39.32 PSV.dial PSR @15:09:40.09 PS.reconfigure :fast @15:09:40.14 PS.seek 2.5ml @15:10:03.66 PSV.dial PTV @15:10:04.43 delay 5 seconds @15:10:09.45 PSV.dial PSR @15:10:10.32 delay 5 seconds @15:10:15.34 PSV.dial PTV @15:10:16.07 delay 5 seconds @15:10:21.09 PSV.dial :air @15:10:22.33 PS.seek 0ml @15:10:45.57 washCycleDA #2 of 3 @15:10:45.58 PSR.select :air @15:10:45.60 PSV.dial PSR @15:10:46.93 Pulling 0.5ml of PSR.wash @15:10:46.94 PSR.select :wash @15:10:47.06 PS.reconfigure :default @15:10:47.11 PS.seek 0.5ml @15:10:53.10 delay 5 seconds @15:10:58.12 PSR.select :air @15:10:58.24 PS.seek 2ml @15:11:14.35 PTV.dial :puck @15:11:14.43 PSV.dial PTV @15:11:15.18 PBV.dial :waste1 @15:11:15.63 PS.seek 1.5ml @15:11:21.57 delay 3 seconds @15:11:24.59 PBV.dial between PSV and waste1 @15:11:24.98 PS.seek 0ml @15:11:41.13 delay 2:00 @15:13:41.16 recoverPSRreagent08 @15:13:41.17 PTV.dial :puck @15:13:41.18 PBV.dial :waste1 @15:13:41.57 delay 30 seconds @15:14:11.60 PBV.dial between PSV and waste1 @15:14:11.99 PSR.select :air @15:14:12.01 PSV.dial PSR @15:14:12.89 PS.reconfigure :fast @15:14:12.94 PS.seek 2.5ml @15:14:36.50 PSV.dial PTV @15:14:37.24 delay 5 seconds @15:14:42.26 PSV.dial PSR @15:14:43.01 delay 5 seconds @15:14:48.03 PSV.dial PTV @15:14:48.77 delay 5 seconds @15:14:53.79 PSV.dial :air @15:14:54.57 PS.seek 0ml @15:15:17.97 washCycleDA #3 of 3 @15:15:17.99 PSR.select :air @15:15:18.00 PSV.dial PSR @15:15:19.33 Pulling 0.5ml of PSR.wash @15:15:19.34 PSR.select :wash @15:15:19.46 PS.reconfigure :default @15:15:19.51 PS.seek 0.5ml @15:15:25.44 delay 5 seconds @15:15:30.46 PSR.select :air @15:15:30.58 PS.seek 2ml @15:15:46.69 PTV.dial :puck @15:15:46.70 PSV.dial PTV @15:15:47.44 PBV.dial :waste1 @15:15:47.89 PS.seek 1.5ml @15:15:53.88 delay 3 seconds @15:15:56.90 PBV.dial between PSV and waste1 @15:15:57.29 PS.seek 0ml @15:16:13.38 delay 2:00 @15:18:13.41 recoverPSRreagent08 @15:18:13.42 PTV.dial :puck @15:18:13.43 PBV.dial :waste1 @15:18:13.80 delay 30 seconds @15:18:43.82 PBV.dial between PSV and waste1 @15:18:44.19 PSR.select :air @15:18:44.21 PSV.dial PSR @15:18:44.96 PS.reconfigure :fast @15:18:45.01 PS.seek 2.5ml @15:19:08.53 PSV.dial PTV @15:19:09.70 delay 5 seconds @15:19:14.78 PSV.dial PSR @15:19:15.53 delay 5 seconds @15:19:20.55 PSV.dial PTV @15:19:21.30 delay 5 seconds @15:19:26.32 PSV.dial :air @15:19:27.10 PS.seek 0ml @15:19:50.31 Gate.power :camera,:ON @15:19:50.33 Mixing DA Substrates @15:19:50.34 PSR.select :air @15:19:50.35 PSV.dial PSR @15:19:51.74 Pulling 0.25ml of PSR.sub1 @15:19:51.76 PSR.select :sub1 @15:19:51.98 PS.reconfigure :default @15:19:52.10 PS.seek 0.25ml @15:19:55.57 delay 5 seconds @15:20:00.59 Pulling 0.25ml of PSR.sub2 @15:20:00.61 PSR.select :sub2 @15:20:00.73 PS.seek 0.5ml @15:20:04.06 delay 5 seconds @15:20:09.08 PSR.select :air @15:20:09.20 PS.seek 2ml @15:20:25.31 PTV.dial :puck @15:20:25.32 PSV.dial PTV @15:20:26.08 PBV.dial :waste1 @15:20:26.53 PS.seek 1.5ml @15:20:32.53 delay 3 seconds @15:20:35.55 PBV.dial between PSV and waste1 @15:20:35.94 PS.seek 0ml @15:20:52.06 delay 10 seconds @15:21:02.09 recoverPSRreagent08 @15:21:02.10 PTV.dial :puck @15:21:02.11 PBV.dial :waste1 @15:21:02.50 delay 30 seconds @15:21:32.53 PBV.dial between PSV and waste1 @15:21:32.92 PSR.select :air @15:21:32.94 PSV.dial PSR @15:21:33.71 PS.reconfigure :fast @15:21:33.76 PS.seek 2.5ml @15:21:57.87 PSV.dial PTV @15:21:58.64 delay 5 seconds @15:22:03.66 PSV.dial PSR @15:22:04.44 delay 5 seconds @15:22:09.46 PSV.dial PTV @15:22:10.22 delay 5 seconds @15:22:15.24 PSV.dial :air @15:22:16.02 PS.seek 0ml @15:22:39.72 Additional air clear @15:22:39.73 PSR.select :air @15:22:39.75 PSV.dial PSR @15:22:41.11 Pulling 2ml of PSR.air @15:22:41.12 PSR.select :air @15:22:41.14 PS.reconfigure :default @15:22:41.19 PS.seek 2ml @15:23:02.40 delay 5 seconds @15:23:07.42 PTV.dial :puck @15:23:07.45 PSV.dial PTV @15:23:08.19 PBV.dial :waste1 @15:23:08.64 PS.seek 1.5ml @15:23:14.53 delay 3 seconds @15:23:17.55 PBV.dial between PSV and waste1 @15:23:17.94 delay 1 second @15:23:18.96 PSV.dial :waste1 @15:23:20.02 PS.seek 0ml @15:23:36.42 Processing Puck Heater at 27.9C before imaging @15:23:36.43 PH.coast @15:23:36.55 Imaging da puck in Processing Clamp while storing it to tube 3 @15:23:37.33 SC.reconfigure :tight @15:23:37.38 SC.seek 3 @15:23:41.05 SC.configure :limp @15:23:41.27 Elbow.reconfigure :tight @15:23:41.32 Elbow.seek 3 @15:23:43.00 Forearm.reconfigure :tight @15:23:43.05 Forearm.seek 3 + 800 counts @15:23:44.09 SE.reconfigure :pick @15:23:44.15 SE.seek :beamBreak @15:23:46.71 SE.configure :default @15:23:46.87 Elbow.reconfigure :limp @15:23:46.93 Forearm.reconfigure :loose @15:23:46.98 Forearm.seek 3 @15:23:47.39 Elbow.hold @15:23:47.46 Elbow.reconfigure :tight @15:23:47.56 SE.seek 5.1 Pucks below Top Plate @15:23:48.02 Forearm.force -20 @15:23:48.06 Forearm.seek 3 + 316 counts @15:23:48.35 Forearm.reconfigure :tight @15:23:48.41 Forearm.seek 3 + 800 counts @15:23:48.70 SE.seek 6.1 Pucks below Top Plate @15:23:49.28 Forearm.reconfigure :loose @15:23:49.33 Forearm.seek :retract @15:23:50.29 Elbow.seek PC @15:23:52.80 Elbow.reconfigure :loose @15:23:52.98 Forearm.seek PC @15:23:54.06 Hand.close @15:23:54.87 PC.seek :open @15:23:59.42 Forearm.seek :retract @15:24:00.43 Elbow.reconfigure :tight @15:24:00.48 Elbow.seek 3 @15:24:03.66 Forearm.reconfigure :tight @15:24:03.71 Forearm.seek 3 + 800 counts @15:24:04.74 Hand.open @15:24:05.55 SE.seek 5.85 Pucks below Top Plate @15:24:05.83 SE.reconfigure :seal @15:24:05.89 SE.seek 5.2 Pucks below Top Plate @15:24:06.53 Lights, camera, ACTION! @15:24:06.57 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da25nov2015h250ml.tif @15:24:25.94 Waiting >20 seconds for da to relinquish FlushPuck @15:24:32.44 SX HX9/16 (USB:12): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 22.57 seconds /var/log/esp/da25nov2015h250ml.tif: TIFF Upload Complete @15:24:32.48 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da25nov2015h250ml15s.tif @15:24:48.12 SX HX9/16 (USB:12): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da25nov2015h250ml15s.tif: TIFF Upload Complete @15:24:48.15 PuckCamera: snap -bin=2,2 21.0 /var/log/esp/midres/da25nov2015h250ml21s.tif @15:25:09.80 SX HX9/16 (USB:12): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21 seconds /var/log/esp/midres/da25nov2015h250ml21s.tif: TIFF Upload Complete @15:25:09.82 Gate.power :camera,:OFF @15:25:09.84 DAfans.email "22.57 sec auto-exposure",Subject: "da25nov2015h250ml images!" @15:25:10.21 Elbow.seek 3 @15:25:10.64 Forearm.seek 3 + 800 counts @15:25:10.93 SE.reconfigure :gently @15:25:10.99 SE.seek 5.91 Pucks below Top Plate @15:25:11.79 Elbow.reconfigure :limp @15:25:11.85 Forearm.reconfigure :loose @15:25:11.90 Forearm.seek 3 @15:25:12.36 Elbow.hold @15:25:12.43 Elbow.reconfigure :tight @15:25:12.49 SE.reconfigure :default @15:25:12.54 SE.seek 6.1 Pucks below Top Plate @15:25:13.13 Forearm.force -20 @15:25:13.19 Forearm.seek 3 + 300 counts @15:25:13.61 Forearm.reconfigure :tight @15:25:13.67 Forearm.seek 3 + 800 counts @15:25:14.11 SE.seek :down @15:25:15.42 Forearm.reconfigure :loose @15:25:15.53 Forearm.seek :retract @15:25:16.77 SE.stop @15:25:17.88 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:25:18.54 Elbow.seek FlushPuck::Garage @15:25:20.42 Elbow.reconfigure :loose @15:25:20.62 Forearm.seek :garage @15:25:21.69 Hand.close @15:25:22.73 Forearm.seek :retract @15:25:23.89 Elbow.reconfigure :tight @15:25:24.01 Elbow.seek PC @15:25:25.49 Elbow.reconfigure :loose @15:25:25.71 Forearm.reconfigure :tight @15:25:25.81 Forearm.seek PC @15:25:27.51 PC.seek :closed @15:25:32.09 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.54 @15:25:32.24 Hand.open @15:25:33.13 Forearm.reconfigure :loose @15:25:33.23 Forearm.seek :retract @15:25:34.33 PSR.select :air @15:25:34.39 Storing wcr puck in Collection Clamp to tube 3 @15:25:34.44 PSV.dial PSR @15:25:34.92 SC.reconfigure :tight @15:25:35.03 SC.seek 3 @15:25:35.30 SC.configure :limp @15:25:35.35 Pulling 0.5ml of PSR.flush @15:25:35.39 PSR.select :flush @15:25:36.78 PS.seek 0.5ml @15:25:37.04 Elbow.reconfigure :tight @15:25:37.23 Elbow.seek 3 @15:25:40.30 Forearm.reconfigure :tight @15:25:40.40 Forearm.seek 3 + 800 counts @15:25:41.56 SE.reconfigure :pick @15:25:41.68 SE.seek :beamBreak @15:25:42.78 delay 5 seconds @15:25:44.21 SE.configure :default @15:25:44.39 Elbow.reconfigure :limp @15:25:44.44 Forearm.reconfigure :loose @15:25:44.50 Forearm.seek 3 @15:25:44.55 upsync @15:25:44.88 Elbow.hold @15:25:44.96 Elbow.reconfigure :tight @15:25:45.07 SE.seek 6.1 Pucks below Top Plate @15:25:45.54 Forearm.force -20 @15:25:45.58 Forearm.seek 3 + 315 counts @15:25:45.95 Forearm.reconfigure :tight @15:25:46.01 Forearm.seek 3 + 800 counts @15:25:46.24 SE.seek 7.1 Pucks below Top Plate @15:25:46.81 Forearm.reconfigure :loose @15:25:46.86 Forearm.seek :retract @15:25:47.80 Elbow.seek CC @15:25:47.86 PSR.select :air @15:25:47.98 PS.seek 2.5ml @15:25:49.37 Elbow.reconfigure :loose @15:25:49.48 Forearm.seek CC @15:25:50.47 Hand.close @15:25:51.63 CC.seek :open @15:25:55.91 Forearm.seek :retract @15:25:56.89 Elbow.reconfigure :tight @15:25:56.95 Elbow.seek 3 @15:25:58.70 Elbow.reconfigure :limp @15:25:58.75 Forearm.seek 3 @15:25:59.89 Elbow.hold @15:25:59.96 Elbow.reconfigure :tight @15:26:00.01 Hand.open @15:26:00.87 Forearm.force -20 @15:26:00.91 Forearm.seek 3 + 318 counts @15:26:01.20 Forearm.reconfigure :tight @15:26:01.26 Forearm.seek 3 + 800 counts @15:26:01.63 SE.seek :down @15:26:02.90 Forearm.reconfigure :loose @15:26:03.01 Forearm.seek :retract @15:26:03.67 SE.stop @15:26:03.93
Starting finalPurge @15:26:09.28 delay 1 second @15:26:10.30 PRV.dial :waste2,avoiding: :ab2 @15:26:12.18 PTV.dial :PRVmixing @15:26:13.50 PSV.dial :mixloop @15:26:14.55 PS.seek :empty @15:26:23.72
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:26:41.12 PSR.select :air @15:26:41.14 PSV.dial PSR @15:26:42.18 Pulling 0.5ml of PSR.flush @15:26:42.19 PSR.select :flush @15:26:42.31 PS.seek 0.5ml @15:26:48.18 delay 5 seconds @15:26:53.20 PSR.select :air @15:26:53.33 PS.seek 2.5ml @15:27:14.53 delay 1 second @15:27:15.55 PBV.dial :waste1 @15:27:16.39 PTV.dial :mixing @15:27:17.14 PSV.dial :mixloop @15:27:18.19 PS.seek :empty @15:27:44.49 PSV.dial PSR @15:27:45.54 PSR.select :flush @15:27:45.66 PS.seek 1ml @15:27:56.79 PSR.select :air @15:27:57.17 PS.seek 2.5ml @15:28:13.37 PSV.dial PTV @15:28:14.12 PTV.dial :puck @15:28:14.85 PBV.dial :waste1 @15:28:14.94 PS.seek 0ml @15:28:41.27 PSV.dial PSR @15:28:42.03 PSR.select :flush @15:28:42.15 PS.seek 0.5ml @15:28:48.02 PSR.select :air @15:28:48.15 PS.seek 2.5ml @15:29:09.37 PSV.dial PTV @15:29:10.12 PBV.dial PSV @15:29:10.70 PS.seek 0ml @15:29:37.02 PSV.dial PBV @15:29:37.61 PS.seek 2.5ml @15:30:03.99 PSV.dial :waste1 @15:30:04.75 PS.seek 0ml @15:30:31.15 PSV.dial PSR @15:30:31.62 PSR.select :air @15:30:31.64 PS.seek 2.5ml @15:30:58.05 PSV.dial PTV @15:30:58.79 PBV.dial PSV @15:30:58.87 PS.seek 0ml @15:31:25.27 PSV.dial PBV @15:31:25.73 PS.seek 2.5ml @15:31:52.12 PSV.dial :waste1 @15:31:52.86 PS.seek 0ml @15:32:19.24 PSV.dial PSR @15:32:19.71 PSR.select :flush @15:32:19.83 PS.seek 1ml @15:32:30.86 PSR.select :air @15:32:30.98 PS.seek 2.5ml @15:32:47.12 PSV.dial PTV @15:32:47.85 PTV.dial :puck @15:32:47.93 PBV.dial :waste1 @15:32:48.96 PS.seek 0ml @15:33:15.24 PSV.dial PSR @15:33:15.99 PSR.select :flush @15:33:16.12 PS.seek 0.5ml @15:33:22.02 PSR.select :air @15:33:22.14 PS.seek 2.5ml @15:33:43.40 PSV.dial PTV @15:33:44.15 PBV.dial PSV @15:33:44.73 PS.seek 0ml @15:34:11.30 PSV.dial PBV @15:34:11.76 PS.seek 2.5ml @15:34:38.05 PSV.dial :waste1 @15:34:38.79 PS.seek 0ml @15:35:05.18 PSV.dial PSR @15:35:05.65 PSR.select :air @15:35:05.67 PS.seek 2.5ml @15:35:32.08 PSV.dial PTV @15:35:32.82 PBV.dial PSV @15:35:32.90 PS.seek 0ml @15:35:59.33 PSV.dial PBV @15:35:59.79 PS.seek 2.5ml @15:36:26.14 PSV.dial :waste1 @15:36:26.89 PS.seek 0ml @15:36:53.34
Loading FlushPuck into Collection Clamp from Processing Clamp @15:36:53.53 Elbow.seek PC @15:36:56.03 Elbow.reconfigure :loose @15:36:56.13 Forearm.seek PC @15:36:57.22 Hand.close @15:36:57.98 PC.seek :open @15:37:02.48 Forearm.seek :retract @15:37:03.51 Elbow.reconfigure :tight @15:37:03.56 Elbow.seek CC @15:37:07.11 Elbow.reconfigure :loose @15:37:07.21 Forearm.reconfigure :tight @15:37:07.25 Forearm.seek CC @15:37:08.92 CC.seek :closed @15:37:12.93 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.04 @15:37:13.00 Hand.open @15:37:13.79 Forearm.reconfigure :loose @15:37:13.84 Forearm.seek :retract @15:37:14.91 Flushing the Collection Valve::Manifold @15:37:15.04 CSV.dial CSR @15:37:15.48 Pulling 5ml of CSR.flush @15:37:15.49 CSR.select :flush @15:37:15.60 CS.seek 5ml @15:37:29.24 delay 5 seconds @15:37:34.26 CSR.select :air @15:37:34.43 CS.reconfigure :fast @15:37:34.48 CS.seek 7.5ml @15:37:41.18 CSV.dial :waste @15:37:42.48 CS.reconfigure :default @15:37:42.53 CS.seek 0ml @15:38:02.49 Flushing lower manifold's waste1 port @15:38:02.62 CSV.dial :flush @15:38:03.06 CS.reconfigure :fast @15:38:03.11 CS.seek 5ml @15:38:15.55 delay 5 seconds @15:38:20.57 CSV.dial :air @15:38:21.21 CS.seek 7ml @15:38:26.77 CSV.dial CTV @15:38:26.80
CTV.dial CSV @15:38:28.23 CBV.dial :waste1 @15:38:29.01 CS.seek 0ml @15:38:45.93 Flushing lower manifold's waste2 port @15:38:46.05 CSV.dial :flush @15:38:46.92 CS.seek 5ml @15:38:59.37 delay 5 seconds @15:39:04.39 CSV.dial :air @15:39:04.90 CS.seek 7ml @15:39:10.34 CSV.dial CTV @15:39:10.36
CTV.dial CSV @15:39:10.82 CBV.dial :waste2 @15:39:11.89 CS.seek 0ml @15:39:29.22 Flushing lower manifold's waste2b port @15:39:29.35 CSV.dial :flush @15:39:30.11 CS.seek 5ml @15:39:42.71 delay 5 seconds @15:39:47.73 CSV.dial :air @15:39:48.38 CS.seek 6.999ml @15:39:53.87 CSV.dial CTV @15:39:53.89
CTV.dial CSV @15:39:54.35 CBV.dial :waste2b @15:39:55.40 CS.seek 0ml @15:40:12.27 Disinfecting external Intake with kill @15:40:12.40 CSV.dial CSR @15:40:12.96 Pulling 8ml of CSR.kill @15:40:12.97 CSR.select :kill @15:40:13.09 CS.seek 8ml @15:40:32.37 delay 5 seconds @15:40:37.39 CSR.select :air @15:40:37.51 CS.seek 10ml @15:40:42.99 CSV.dial CBV @15:40:43.03
CBV.dial CSV @15:40:44.08 CTV.dial :intake @15:40:44.86 Intake.select :open @15:40:44.98 CS.seek 2ml @15:41:04.15 Intake.select :close @15:41:04.25 CTV.dial :air @15:41:05.03 CS.reconfigure :default @15:41:05.07 CS.seek 0ml @15:41:11.05 CSV.dial CSR @15:41:12.07 Pulling 5ml of CSR.flush @15:41:12.08 CSR.select :flush @15:41:12.19 CS.seek 5ml @15:41:25.77 delay 5 seconds @15:41:30.79 CSR.select :air @15:41:30.91 CS.seek 10ml @15:41:44.55 CSV.dial CBV @15:41:45.57 CS.seek 0ml @15:42:12.02 Garaging FlushPuck in Collection Clamp @15:42:12.09 Elbow.reconfigure :tight @15:42:12.14 Elbow.seek CC @15:42:12.26 Elbow.reconfigure :loose @15:42:12.36 Forearm.seek CC @15:42:13.36 Hand.close @15:42:14.10 CC.seek :open @15:42:18.23 Forearm.seek :retract @15:42:19.22 Elbow.reconfigure :tight @15:42:19.27 Elbow.seek :garage @15:42:22.60 Elbow.reconfigure :loose @15:42:22.71 Forearm.reconfigure :tight @15:42:22.75 Forearm.seek :garage @15:42:23.84 Hand.open @15:42:24.66 Forearm.reconfigure :loose @15:42:24.71 Forearm.seek :retract @15:42:25.72 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:41:03 @15:42:25.77 Asleep after completing DA_WCR_112025 assays @15:42:25.79 GoodNews.email "{1 => 22,\n 2 => 22,\n 3 => 8,\n 4 => 14,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@15:40:26, 41% flow, 18.8C, 26% humidity, 14.5psia, 13.65V, 0.372A avg, 331.877Ah, 5.07W\nSampled 170.1ml",Subject: "Asleep after completing DA_WCR_112025 assays" @15:42:29.61 upsync + ESPwhich DA_WCR_112525.rb + fn=DA_WCR_112525.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_112525.rb + [ -f /home/esp/esp2/mission/DA_WCR_112525.rb ] + realpath /home/esp/esp2/mission/DA_WCR_112525.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_112525.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_112525.rb @13:43:52.10UTC25-Nov-25
ESP2 v3.42 I2C Gateway v3.20 for core bus @13:43:54.67 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @13:43:58.62 Polling Can every 10:00 @13:43:58.72 Cleanly exited at 13:43:35UTC25-Nov-25 @13:43:58.77 Loading /home/esp/esp2local/mission/DA_WCR_112525.rb mission @13:43:58.82 Preparing to sleep until + 00:00:05 @13:43:58.83 GoodNews.email "Starting DA_WCR_112525 w/pucks in tube 4\n{1 => 22,\n 2 => 22,\n 3 => 8,\n 4 => 14,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@13:43:58, 42% flow, 18.0C, 27% humidity, 14.5psia, 13.65V, 0.321A avg, 355.289Ah, 4.39W",Subject: "DA_WCR_112525 begun" @13:43:58.88
Asleep til + 00:00:05 @13:43:58.92 Continuing at 13:44 @13:43:59.16 Gate.power :core,:OFF @13:43:59.17 Gate.power :raw,:OFF @13:43:59.18 Shutdown @13:44:01.89 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112525.rb:14\n{1 => 22,\n 2 => 22,\n 3 => 8,\n 4 => 14,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@13:43:58, 42% flow, 18.0C, 27% humidity, 14.5psia, 13.65V, 0.328A avg, 355.289Ah, 4.47W",Subject: "Asleep until 13:44" @13:44:03.37 upsync @13:44:03.82
Powering core bus @13:44:06.16 upsync @13:44:09.00
Gate.power :raw,:ON @13:44:10.09 CS.configure :default @13:44:10.13 PS.configure :default @13:44:10.18 SS.configure :default @13:44:10.23 CC.configure :default @13:44:10.27 PC.configure :default @13:44:10.34 Forearm.configure :loose @13:44:10.39 Elbow.configure :loose @13:44:10.46 SC.configure :loose @13:44:10.51 SE.configure :default @13:44:10.56 CH.configure :default @13:44:10.59 PH.configure :default @13:44:10.63 All dwarf controllers running firmware version 3.85 @13:44:11.07 Valve.reset @13:44:11.36 Intake.select :close @13:44:11.39 Exhaust.select :close @13:44:11.42 CTV.dial :air @13:44:11.54 PSV.dial :air @13:44:11.59 CSV.dial :waste1 @13:44:11.69
Forearm.home.seek :retract @13:44:12.19 SSV.dial :spit,avoiding: :exhaust @13:44:12.42 Hand.close @13:44:12.45
Elbow.reconfigure :loose @13:44:12.55 Elbow.jog 1500 @13:44:12.66 PS.home.seek 0ml @13:44:13.05 CS.reconfigure :fast @13:44:13.08 SS.home.seek 0ml @13:44:13.17 CS.home.seek 0ml @13:44:13.24
Elbow.reconfigure :loose @13:44:13.31 Elbow.home.seek :clear @13:44:16.56 SE.home.seek :down @13:44:17.18 SC.reconfigure :tight @13:44:17.23 SC.home.seek :initial @13:44:21.82 PS.stop @13:44:33.11
SC.configure :limp @13:44:33.17 Forearm.seek :retract @13:44:33.23 Elbow.seek :hammer @13:44:34.26 Forearm.reconfigure :tight @13:44:34.31 Forearm.seek retracted @13:44:34.59 PC.home.seek :open @13:44:34.62
CC.home.seek :open @13:44:37.81 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @13:44:37.83 Notice.email "At 13:44:37 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_112525.rb:14\n{1 => 22,\n 2 => 22,\n 3 => 8,\n 4 => 14,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@13:44:37, 42% flow, 18.0C, 27% humidity, 14.5psia, 13.68V, 0.290A avg, 355.293Ah, 3.97W",Subject: "Started DA_WCR_112525 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @13:44:37.85
Starting initialPurge @13:44:37.88 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:44:37.94 Hand.open @13:44:38.78 Elbow.reconfigure :tight @13:44:38.83 Elbow.seek FlushPuck::Garage @13:44:40.78 Elbow.reconfigure :loose @13:44:40.99 Forearm.reconfigure :loose @13:44:41.07 Forearm.seek :garage @13:44:42.02 Hand.close @13:44:42.24 upsync @13:44:42.89
Forearm.seek :retract @13:44:43.86 Elbow.reconfigure :tight @13:44:43.91 Elbow.seek CC @13:44:46.74 Elbow.reconfigure :loose @13:44:46.85 Forearm.reconfigure :tight @13:44:46.89 Forearm.seek CC @13:44:48.22 CC.seek :closed @13:44:52.23 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.04 @13:44:52.30 Hand.open @13:44:53.08 Forearm.reconfigure :loose @13:44:53.12 Forearm.seek :retract @13:44:54.14 Flushing the Collection Valve::Manifold @13:44:54.26 CSV.dial CSR @13:44:55.56 Pulling 5ml of CSR.flush @13:44:55.57 CSR.select :flush @13:44:55.69 CS.reconfigure :default @13:44:55.74 CS.seek 5ml @13:45:09.83 delay 5 seconds @13:45:14.85 CSR.select :air @13:45:15.01 CS.reconfigure :fast @13:45:15.05 CS.seek 7.5ml @13:45:22.05 CSV.dial :waste @13:45:23.33 CS.reconfigure :default @13:45:23.38 CS.seek 0ml @13:45:43.33 Purging external Intake @13:45:43.45 CSV.dial :flush @13:45:43.91 CS.reconfigure :fast @13:45:43.96 CS.seek 10ml @13:46:08.08 delay 5 seconds @13:46:13.10 CSV.dial CBV @13:46:13.13
CBV.dial CSV @13:46:13.87 CTV.dial :intake @13:46:14.65 Intake.select :open @13:46:14.76 CS.seek 0ml @13:46:38.83 Intake.select :close @13:46:38.93 CTV.dial :air @13:46:39.72 Flushing upper manifold @13:46:39.84 CSV.dial :flush @13:46:40.57 CS.seek 5ml @13:46:53.14 delay 5 seconds @13:46:58.16 CSV.dial :air @13:46:58.69 CS.seek 7ml @13:47:04.14 CSV.dial CBV @13:47:04.17
CBV.dial CSV @13:47:05.33 CTV.dial :air @13:47:05.41 CS.seek 0ml @13:47:22.48 delay 7 seconds @13:47:29.51 CS.seek 3ml @13:47:37.26 CSV.dial :waste @13:47:37.71 CS.seek 0ml @13:47:45.55 CSV.dial :flush @13:47:45.99 CS.seek 3ml @13:47:53.82 delay 5 seconds @13:47:58.84 CSV.dial :air @13:47:59.35 CS.seek 5.001ml @13:48:04.83 CSV.dial :air @13:48:05.02 CS.seek 0ml @13:48:17.32 delay 7 seconds @13:48:24.35 CS.seek 3ml @13:48:32.10 CSV.dial :waste @13:48:32.88 CS.seek 0ml @13:48:40.51 Garaging FlushPuck in Collection Clamp @13:48:40.58 Elbow.reconfigure :tight @13:48:40.63 Elbow.seek CC @13:48:40.75 Elbow.reconfigure :loose @13:48:40.85 Forearm.seek CC @13:48:41.79 Hand.close @13:48:42.52 CC.seek :open @13:48:47.01 Forearm.seek :retract @13:48:48.09 Elbow.reconfigure :tight @13:48:48.14 Elbow.seek :garage @13:48:51.00 Elbow.reconfigure :loose @13:48:51.10 Forearm.reconfigure :tight @13:48:51.15 Forearm.seek :garage @13:48:52.17 Hand.open @13:48:52.95 Forearm.reconfigure :loose @13:48:52.99 Forearm.seek :retract @13:48:54.00 da sampling at most 1000ml, wcr at most 1000ml @13:48:54.04 Loading sh1 puck from tube 4 into Collection Clamp @13:48:54.05 DAfans.email "Started da in tube 4 at 13:48:54UTC25-Nov-25\n{1 => 22,\n 2 => 22,\n 3 => 8,\n 4 => 14,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@13:44:37, 42% flow, 18.0C, 27% humidity, 14.5psia, 13.68V, 0.290A avg, 355.293Ah, 3.97W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @13:48:54.34
SC.reconfigure :tight @13:48:54.38 SC.seek 4 @13:48:54.46 SC.configure :limp @13:48:54.70 Elbow.reconfigure :tight @13:48:54.76 Elbow.seek 4 @13:48:56.66 Forearm.reconfigure :tight @13:48:56.71 Forearm.seek 4 + 800 counts @13:48:57.75 SE.reconfigure :pick @13:48:57.82 SE.seek :beamBreak @13:48:58.37 upsync @13:48:59.54
SE.configure :default @13:48:59.71 Elbow.seek 4 @13:48:59.89 Forearm.seek 4 + 300 counts @13:49:00.34 Forearm.reconfigure :loose @13:49:00.39 Hand.close @13:49:01.15 SE.seek :down @13:49:01.79
Forearm.seek :retract @13:49:02.70 SE.stop @13:49:02.80
Elbow.seek CC @13:49:04.33 Elbow.reconfigure :loose @13:49:04.43 Forearm.reconfigure :tight @13:49:04.48 Forearm.seek CC @13:49:05.81 CC.seek :closed @13:49:09.80 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.11 @13:49:09.81 Hand.open @13:49:10.57 Forearm.reconfigure :loose @13:49:10.61 Forearm.seek :retract @13:49:11.58 collectSample 1000ml w/bubble point of 24psi @13:49:11.59 Priming 7ml @13:49:11.60 Intake.select :close @13:49:11.61 Exhaust.select :close @13:49:11.62 SS.hold @13:49:11.82 Calibrating pressure and temperature sensors @13:49:11.85 CTV.dial :air @13:49:11.93 SSV.dial :spit,avoiding: :exhaust @13:49:11.95 SS.reconfigure :fast @13:49:12.00 SS.seek 5ml @13:49:15.13 SSV.dial CBV @13:49:15.17
CBV.dial SSV @13:49:16.52 delay 3.8 seconds @13:49:20.45 Sensor limits: Intake max 91.6psia; Outlet max 108.5psia @13:49:20.46 SSV.dial :spit @13:49:21.82 SS.seek 0ml @13:49:24.38 SSV.dial CBV @13:49:25.75 CTV.dial :intake @13:49:26.53 SSV.dial CBV @13:49:26.55
CBV.dial SSV @13:49:26.79 Allowing 40s for 7.0ml of remaining 7.0ml @13:49:26.80 Intake.select :open @13:49:28.97 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:49:28.99 SS.reconfigure :inhaling 2.17ml/s @13:49:29.03 SS.seek 7ml @13:49:35.59 Stroke completed -- waiting for pressure to equalize ... @13:49:35.60 SS.reconfigure :exhale @13:49:35.65 SS.hold @13:49:36.26 Intake.select :close @13:49:36.42 SSV.dial :exhaust @13:49:37.21 Exhaust.select :open @13:49:37.74 SS.seek 0ml @13:49:41.69 Exhaust.select :close @13:49:41.80 Sampling 1000.0ml @13:49:41.81 Intake.select :close @13:49:41.82 Exhaust.select :close @13:49:41.84 SS.reconfigure :default @13:49:41.88 SS.hold @13:49:42.08 CTV.dial :intake @13:49:42.15 SSV.dial CBV @13:49:42.18
CBV.dial SSV @13:49:42.99 Allowing 130s for 25.0ml of remaining 1000.0ml @13:49:43.00 Intake.select :open @13:49:45.16 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:49:45.18 SS.reconfigure :inhaling 2.17ml/s @13:49:45.22 SS.seek 25ml @13:50:00.17 Stroke completed -- waiting for pressure to equalize ... @13:50:00.18 SS.reconfigure :exhale @13:50:00.23 SS.hold @13:50:08.77 Intake.select :close @13:50:08.92 SSV.dial :exhaust @13:50:09.69 Exhaust.select :open @13:50:09.80 SS.seek 0ml @13:50:18.67 Exhaust.select :close @13:50:18.78 SSV.dial CBV @13:50:18.81
CBV.dial SSV @13:50:19.59 Allowing 130s for 25.0ml of remaining 975.0ml @13:50:19.61 Intake.select :open @13:50:21.77 Intake @13.9psia, vacuum from 8.4 to 11.4psi @13:50:21.79 SS.reconfigure :inhaling 2.17ml/s @13:50:21.83 SS.seek 25ml @13:50:37.16 Stroke completed -- waiting for pressure to equalize ... @13:50:37.17 SS.reconfigure :exhale @13:50:37.21 SS.hold @13:50:46.11 Intake.select :close @13:50:46.27 SSV.dial :exhaust @13:50:47.03 Exhaust.select :open @13:50:47.14 SS.seek 0ml @13:50:56.47 Exhaust.select :close @13:50:56.58 SSV.dial CBV @13:50:56.60
CBV.dial SSV @13:50:57.41 Allowing 130s for 25.0ml of remaining 950.0ml @13:50:57.42 Intake.select :open @13:50:59.58 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:50:59.60 SS.reconfigure :inhaling 2.17ml/s @13:50:59.65 SS.seek 25ml @13:51:13.51 Stroke completed -- waiting for pressure to equalize ... @13:51:13.52 SS.reconfigure :exhale @13:51:13.57 SS.hold @13:51:23.47 Intake.select :close @13:51:23.62 SSV.dial :exhaust @13:51:24.40 Exhaust.select :open @13:51:24.51 SS.seek 0ml @13:51:33.84 Exhaust.select :close @13:51:33.95 SSV.dial CBV @13:51:33.97
CBV.dial SSV @13:51:34.77 Allowing 130s for 25.0ml of remaining 925.0ml @13:51:34.78 Intake.select :open @13:51:36.94 Intake @13.3psia, vacuum from 7.8 to 10.8psi @13:51:36.96 SS.reconfigure :inhaling 2.17ml/s @13:51:37.00 SS.seek 25ml @13:51:49.44 Vacuum too high -- waiting up to 40 seconds @13:51:49.46 SS.reconfigure :exhale @13:51:49.51 SS.hold @13:51:50.18 Intake @10.0psia, vacuum from 4.5 to 7.5psi @13:51:50.20 SS.reconfigure :inhaling 1.95ml/s @13:51:50.24 SS.seek 25ml @13:51:52.34 Stroke completed -- waiting for pressure to equalize ... @13:51:52.35 SS.reconfigure :exhale @13:51:52.40 SS.hold @13:52:02.21 Intake.select :close @13:52:02.37 SSV.dial :exhaust @13:52:03.13 Exhaust.select :open @13:52:03.67 SS.seek 0ml @13:52:12.73 Exhaust.select :close @13:52:12.84 SSV.dial CBV @13:52:12.86
CBV.dial SSV @13:52:13.69 Allowing 130s for 25.0ml of remaining 900.0ml @13:52:13.70 Intake.select :open @13:52:15.86 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:52:15.88 SS.reconfigure :inhaling 1.95ml/s @13:52:15.93 SS.seek 25ml @13:52:23.26 Vacuum too high -- waiting up to 40 seconds @13:52:23.27 SS.reconfigure :exhale @13:52:23.32 SS.hold @13:52:23.95 Intake @10.8psia, vacuum from 5.3 to 8.3psi @13:52:23.96 SS.reconfigure :inhaling 1.77ml/s @13:52:24.01 SS.seek 25ml @13:52:28.42 Vacuum too high -- waiting up to 40 seconds @13:52:28.43 SS.reconfigure :exhale @13:52:28.49 SS.hold @13:52:34.38 Intake @11.4psia, vacuum from 5.9 to 8.9psi @13:52:34.40 SS.reconfigure :inhaling 1.59ml/s @13:52:34.44 SS.seek 25ml @13:52:40.90 Stroke completed -- waiting for pressure to equalize ... @13:52:40.91 SS.reconfigure :exhale @13:52:41.01 SS.hold @13:52:46.63 Intake.select :close @13:52:46.78 SSV.dial :exhaust @13:52:47.55 Exhaust.select :open @13:52:47.66 SS.seek 0ml @13:52:56.90 Exhaust.select :close @13:52:57.01 SSV.dial CBV @13:52:57.03
CBV.dial SSV @13:52:57.83 Allowing 130s for 25.0ml of remaining 875.0ml @13:52:57.84 Intake.select :open @13:53:00.00 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:53:00.02 SS.reconfigure :inhaling 1.59ml/s @13:53:00.07 SS.seek 25ml @13:53:08.25 Vacuum too high -- waiting up to 40 seconds @13:53:08.27 SS.reconfigure :exhale @13:53:08.32 SS.hold @13:53:10.14 Intake @11.4psia, vacuum from 5.9 to 8.9psi @13:53:10.16 SS.reconfigure :inhaling 1.43ml/s @13:53:10.20 SS.seek 25ml @13:53:15.32 Vacuum too high -- waiting up to 40 seconds @13:53:15.33 SS.reconfigure :exhale @13:53:15.38 SS.hold @13:53:23.36 Intake @12.5psia, vacuum from 7.0 to 10.0psi @13:53:23.45 SS.reconfigure :inhaling 1.29ml/s @13:53:23.50 SS.seek 25ml @13:53:31.04 Stroke completed -- waiting for pressure to equalize ... @13:53:31.05 SS.reconfigure :exhale @13:53:31.10 SS.hold @13:53:37.64 Intake.select :close @13:53:37.79 SSV.dial :exhaust @13:53:38.56 Exhaust.select :open @13:53:38.67 SS.seek 0ml @13:53:47.90 Exhaust.select :close @13:53:48.01 SSV.dial CBV @13:53:48.05
CBV.dial SSV @13:53:48.81 Allowing 130s for 25.0ml of remaining 850.0ml @13:53:48.82 Intake.select :open @13:53:50.98 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:53:51.00 SS.reconfigure :inhaling 1.29ml/s @13:53:51.05 SS.seek 25ml @13:54:00.04 Vacuum too high -- waiting up to 40 seconds @13:54:00.05 SS.reconfigure :exhale @13:54:00.10 SS.hold @13:54:02.29 Intake @11.8psia, vacuum from 6.3 to 9.3psi @13:54:02.30 SS.reconfigure :inhaling 1.16ml/s @13:54:02.35 SS.seek 25ml @13:54:06.07 Vacuum too high -- waiting up to 40 seconds @13:54:06.08 SS.reconfigure :exhale @13:54:06.13 SS.hold @13:54:11.51 Intake @10.6psia, vacuum from 5.1 to 8.1psi @13:54:11.53 SS.reconfigure :inhaling 1.04ml/s @13:54:11.58 SS.seek 25ml @13:54:17.91 Vacuum too high -- waiting up to 40 seconds @13:54:17.92 SS.reconfigure :exhale @13:54:17.97 SS.hold @13:54:24.55 Intake @11.1psia, vacuum from 5.6 to 8.6psi @13:54:24.57 SS.reconfigure :inhaling 0.94ml/s @13:54:24.62 SS.seek 25ml @13:54:30.95 Stroke completed -- waiting for pressure to equalize ... @13:54:30.96 SS.reconfigure :exhale @13:54:31.00 SS.hold @13:54:36.99 Intake.select :close @13:54:37.14 SSV.dial :exhaust @13:54:37.90 Exhaust.select :open @13:54:38.01 SS.seek 0ml @13:54:46.95 Exhaust.select :close @13:54:47.11 SSV.dial CBV @13:54:47.14
CBV.dial SSV @13:54:47.93 Allowing 130s for 25.0ml of remaining 825.0ml @13:54:47.94 Intake.select :open @13:54:50.10 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:54:50.12 SS.reconfigure :inhaling 0.94ml/s @13:54:50.17 SS.seek 25ml @13:54:59.66 Vacuum too high -- waiting up to 40 seconds @13:54:59.67 SS.reconfigure :exhale @13:54:59.72 SS.hold @13:55:03.97 Intake @12.2psia, vacuum from 6.7 to 9.7psi @13:55:03.99 SS.reconfigure :inhaling 0.85ml/s @13:55:04.03 SS.seek 25ml @13:55:08.66 Vacuum too high -- waiting up to 40 seconds @13:55:08.67 SS.reconfigure :exhale @13:55:08.72 SS.hold @13:55:15.41 Intake @12.1psia, vacuum from 6.6 to 9.6psi @13:55:15.43 SS.reconfigure :inhaling 0.77ml/s @13:55:15.48 SS.seek 25ml @13:55:24.47 Vacuum too high -- waiting up to 40 seconds @13:55:24.48 SS.reconfigure :exhale @13:55:24.53 SS.hold @13:55:30.28 Intake @11.6psia, vacuum from 6.1 to 9.1psi @13:55:30.30 SS.reconfigure :inhaling 0.70ml/s @13:55:30.35 SS.seek 25ml @13:55:36.97 Vacuum too high -- waiting up to 40 seconds @13:55:36.98 SS.reconfigure :exhale @13:55:37.03 SS.hold @13:55:46.91 Intake @13.6psia, vacuum from 8.1 to 11.1psi @13:55:46.92 SS.reconfigure :inhaling 0.62ml/s @13:55:46.97 SS.seek 25ml @13:55:51.84 Stroke completed -- waiting for pressure to equalize ... @13:55:51.85 SS.reconfigure :exhale @13:55:51.89 SS.hold @13:56:00.44 Intake.select :close @13:56:00.59 SSV.dial :exhaust @13:56:01.35 Exhaust.select :open @13:56:01.47 SS.seek 0ml @13:56:10.31 Exhaust.select :close @13:56:10.42 SSV.dial CBV @13:56:10.44
CBV.dial SSV @13:56:11.25 Allowing 130s for 25.0ml of remaining 800.0ml @13:56:11.26 Intake.select :open @13:56:13.42 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:56:13.44 SS.reconfigure :inhaling 0.62ml/s @13:56:13.48 SS.seek 25ml @13:56:24.69 Vacuum too high -- waiting up to 40 seconds @13:56:24.70 SS.reconfigure :exhale @13:56:24.75 SS.hold @13:56:31.76 Intake @13.5psia, vacuum from 8.0 to 11.0psi @13:56:31.78 SS.reconfigure :inhaling 0.56ml/s @13:56:31.83 SS.seek 25ml @13:56:39.31 Vacuum too high -- waiting up to 40 seconds @13:56:39.33 SS.reconfigure :exhale @13:56:39.38 SS.hold @13:56:50.63 Intake @13.3psia, vacuum from 7.8 to 10.8psi @13:56:50.64 SS.reconfigure :inhaling 0.51ml/s @13:56:51.12 SS.seek 25ml @13:57:03.06 Vacuum too high -- waiting up to 40 seconds @13:57:03.07 SS.reconfigure :exhale @13:57:03.13 SS.hold @13:57:14.24 Intake @13.4psia, vacuum from 7.9 to 10.9psi @13:57:14.26 SS.reconfigure :inhaling 0.46ml/s @13:57:14.31 SS.seek 25ml @13:57:28.22 Vacuum too high -- waiting up to 40 seconds @13:57:28.23 SS.reconfigure :exhale @13:57:28.28 SS.hold @13:57:41.08 SS.reconfigure :inhaling 0.42ml/s @13:57:41.13 SS.seek 25ml @13:57:47.12 Stroke completed -- waiting for pressure to equalize ... @13:57:47.13 SS.reconfigure :exhale @13:57:47.17 SS.hold @13:57:56.93 Intake.select :close @13:57:57.08 SSV.dial :exhaust @13:57:57.84 Exhaust.select :open @13:57:57.95 SS.seek 0ml @13:58:07.26 Exhaust.select :close @13:58:07.37 SSV.dial CBV @13:58:07.39
CBV.dial SSV @13:58:08.18 Allowing 130s for 25.0ml of remaining 775.0ml @13:58:08.19 Intake.select :open @13:58:10.36 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:58:10.37 SS.reconfigure :inhaling 0.42ml/s @13:58:10.42 SS.seek 25ml @13:58:30.59 Vacuum too high -- waiting up to 40 seconds @13:58:30.60 SS.reconfigure :exhale @13:58:30.65 SS.hold @13:58:39.59 Intake @13.6psia, vacuum from 8.1 to 11.1psi @13:58:39.61 SS.reconfigure :inhaling 0.38ml/s @13:58:39.66 SS.seek 25ml @13:58:54.90 Vacuum too high -- waiting up to 40 seconds @13:58:54.92 SS.reconfigure :exhale @13:58:54.97 SS.hold @13:59:06.14 Intake @13.7psia, vacuum from 8.2 to 11.2psi @13:59:06.16 SS.reconfigure :inhaling 0.33ml/s @13:59:06.21 SS.seek 25ml @13:59:29.69 Vacuum too high -- waiting up to 40 seconds @13:59:29.70 SS.reconfigure :exhale @13:59:29.75 SS.hold @13:59:40.98 Intake @13.6psia, vacuum from 8.1 to 11.1psi @13:59:40.99 SS.reconfigure :inhaling 0.30ml/s @13:59:41.04 SS.seek 25ml @13:59:53.82 Stroke completed -- waiting for pressure to equalize ... @13:59:53.83 SS.reconfigure :exhale @13:59:53.87 SS.hold @14:00:06.43 Intake.select :close @14:00:06.59 SSV.dial :exhaust @14:00:07.35 Exhaust.select :open @14:00:07.46 SS.seek 0ml @14:00:16.73 Exhaust.select :close @14:00:16.83 SSV.dial CBV @14:00:16.86
CBV.dial SSV @14:00:17.65 Allowing 130s for 25.0ml of remaining 750.0ml @14:00:17.66 Intake.select :open @14:00:19.82 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:00:19.84 SS.reconfigure :inhaling 0.30ml/s @14:00:19.89 SS.seek 25ml @14:01:06.15 Vacuum too high -- waiting up to 40 seconds @14:01:06.16 SS.reconfigure :exhale @14:01:06.22 SS.hold @14:01:17.78 Intake @13.8psia, vacuum from 8.3 to 11.3psi @14:01:17.87 SS.reconfigure :inhaling 0.28ml/s @14:01:17.92 SS.seek 25ml @14:01:50.99 Vacuum too high -- waiting up to 40 seconds @14:01:51.01 SS.reconfigure :exhale @14:01:51.06 SS.hold @14:02:07.50 Intake @13.9psia, vacuum from 8.4 to 11.4psi @14:02:07.52 SS.reconfigure :inhaling 0.25ml/s @14:02:07.57 SS.seek 25ml @14:02:20.05 Stroke completed -- waiting for pressure to equalize ... @14:02:20.06 SS.reconfigure :exhale @14:02:20.10 SS.hold @14:02:27.67 SS.reconfigure :default @14:02:27.71 SS.hold @14:02:27.77 Intake.select :close @14:02:27.93 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 275.0ml @14:02:27.94 SSV.dial :exhaust @14:02:28.71 Exhaust.select :open @14:02:28.82 SS.reconfigure :exhale @14:02:28.87 SS.seek 0ml @14:02:38.18 Exhaust.select :close @14:02:38.28 Sampled 275.0ml @14:02:38.30 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @14:02:38.31 Intake.select :close @14:02:38.37 CSV.dial :waste @14:02:38.53 SSV.dial CBV,avoiding: :exhaust @14:02:38.56
CBV.dial SSV @14:02:38.59 CS.seek 0ml @14:02:38.75 CSV.dial :air @14:02:39.49 CS.seek 10ml @14:02:40.57
CTV.dial :air @14:02:41.35 delay 15 seconds @14:02:56.36 SSV.dial :spit,avoiding: :exhaust @14:02:57.73 CTV.dial CSV,avoiding: :intake @14:02:57.76
SS.seek 0ml @14:02:57.88 SSV.dial CBV,avoiding: :exhaust @14:03:03.69 SSV.dial between CBV and plug @14:03:04.19 CBV.dial between SSV and waste2 @14:03:04.59 CTV.dial CSV,avoiding: :air @14:03:04.62
CSV.dial CTV @14:03:05.07 CS.reconfigure :pressurize @14:03:05.12 CS.seek 0ml @14:03:28.91 BubblePoint: Intake @24.2psia, Outlet @0.2psia @14:03:28.92 CBV.dial SSV @14:03:29.25 SS.reconfigure :default @14:03:29.30 SS.seek 10ml @14:03:34.83 delay 15 seconds @14:03:49.96 Evacuated: Intake @23.7psia, Outlet @23.4psia @14:03:49.97 SSV.dial :spit,avoiding: :exhaust @14:03:51.05 SS.reconfigure :exhale @14:03:51.10 SS.seek 0ml @14:03:56.33 SS.reconfigure :fast @14:03:56.38 SS.seek 5ml @14:03:59.62 SSV.dial CBV,avoiding: :exhaust @14:03:59.64
CBV.dial SSV @14:04:01.02 CTV.dial :air,avoiding: :intake @14:04:02.41 CSV.dial CTV @14:04:02.44
SS.seek 10ml @14:04:03.58 CSV.dial :air @14:04:04.04 CS.reconfigure :fast @14:04:04.08 CS.seek 0ml @14:04:05.50
SSV.dial :spit,avoiding: :exhaust @14:04:06.87 SS.reconfigure :exhale @14:04:06.91 SS.seek 0ml @14:04:07.06 CS.stop @14:04:11.87
SS.reconfigure :fast @14:04:11.93 SS.seek 5ml @14:04:14.83 SSV.dial CBV,avoiding: :exhaust @14:04:14.86
CBV.dial SSV @14:04:17.24 SSV.dial :spit,avoiding: :exhaust @14:04:18.60 SS.reconfigure :default @14:04:18.65 SS.seek 0ml @14:04:21.91 SS.stop @14:04:21.95 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @14:04:22.02 CSV.dial :waste @14:04:22.14 SSV.dial CBV,avoiding: :exhaust @14:04:22.17
CBV.dial SSV @14:04:22.82 CS.seek 0ml @14:04:22.88 CSV.dial :air @14:04:23.55
CTV.dial :air @14:04:23.63 CS.seek 10ml @14:04:23.73
delay 15 seconds @14:04:38.75 SSV.dial :spit,avoiding: :exhaust @14:04:40.12 CTV.dial CSV,avoiding: :intake @14:04:40.15
SS.reconfigure :exhale @14:04:40.25 SS.seek 0ml @14:04:40.42 SSV.dial CBV,avoiding: :exhaust @14:04:47.72 SSV.dial between CBV and plug @14:04:48.21 CBV.dial between SSV and waste2 @14:04:48.61 CSV.dial between air and CTV @14:04:48.92 CS.seek 8.055ml @14:04:54.19 CTV.dial CSV,avoiding: :air @14:04:54.22
CSV.dial CTV @14:04:54.57 CS.reconfigure :pressurize @14:04:54.62 CS.seek 0ml @14:05:13.90 BubblePoint: Intake @24.0psia, Outlet @-0.4psia @14:05:13.91 CBV.dial SSV @14:05:14.27 SS.reconfigure :default @14:05:14.32 SS.seek 10ml @14:05:19.75 delay 15 seconds @14:05:34.88 Evacuated: Intake @22.6psia, Outlet @15.1psia @14:05:34.89 SSV.dial :spit,avoiding: :exhaust @14:05:35.98 SS.reconfigure :exhale @14:05:36.02 SS.seek 0ml @14:05:41.23 SS.reconfigure :fast @14:05:41.27 SS.seek 5ml @14:05:43.82 SSV.dial CBV,avoiding: :exhaust @14:05:43.84
CBV.dial SSV @14:05:45.22 CTV.dial :air,avoiding: :intake @14:05:46.60 CSV.dial CTV @14:05:46.63
SS.seek 10ml @14:05:47.85 CSV.dial :air @14:05:48.28 CS.reconfigure :fast @14:05:48.33 CS.seek 0ml @14:05:49.11
SSV.dial :spit,avoiding: :exhaust @14:05:50.47 SS.reconfigure :exhale @14:05:50.52 SS.seek 0ml @14:05:51.28 CS.stop @14:05:55.54
SS.reconfigure :fast @14:05:55.59 SS.seek 5ml @14:05:58.38 SSV.dial CBV,avoiding: :exhaust @14:05:58.42
CBV.dial SSV @14:06:00.76 SSV.dial :spit,avoiding: :exhaust @14:06:02.11 SS.reconfigure :default @14:06:02.16 SS.seek 0ml @14:06:05.34 SS.stop @14:06:05.38 Lysing: Adding 2.0ml AqMeOH @14:06:05.39 CBV.dial between CSV and waste1,avoiding: :DBV @14:06:06.90 CTV.dial CSV @14:06:08.26 CSV.dial CSR @14:06:09.05 Pulling 1ml of CSR.AqMeOH @14:06:09.06 CSR.select :AqMeOH @14:06:09.18 CS.reconfigure :default @14:06:09.22 CS.seek 1ml @14:06:12.57 delay 5 seconds @14:06:17.59 CSR.select :air @14:06:17.76 CS.seek 6ml @14:06:31.40 delay 2 seconds @14:06:33.42 CSV.dial :waste2 @14:06:33.86 CS.reconfigure :fast @14:06:33.91 CS.seek 0ml @14:06:48.43 CSV.dial CSR @14:06:48.89 Pulling 2ml of CSR.AqMeOH @14:06:48.90 CSR.select :AqMeOH @14:06:49.01 CS.reconfigure :default @14:06:49.06 CS.seek 2ml @14:06:55.37 delay 5 seconds @14:07:00.39 CSR.select :air @14:07:00.56 CS.seek 5ml @14:07:09.09 delay 2 seconds @14:07:11.11 CSV.dial CTV @14:07:11.55 CS.seek 1ml @14:07:22.67 Before lysing Collection Puck Heater at 13.5C/15.6psia @14:07:22.69 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @14:07:22.70 CH.seek 70C,8:00 @14:09:53.85 CH.hold 10:00,70C @14:09:54.04 Begun lysing Collection Puck Heater at 68.1C/22.4psia @14:19:54.05 Ended lysing Collection Puck Heater at 70C/18.6psia @14:19:54.06 CS.seek 0ml @14:19:57.46 CTV.dial between spare and air,avoiding: :intake @14:19:58.53 CSV.dial :air @14:19:59.00 CS.seek 5ml @14:20:12.71 CSV.dial CTV @14:20:13.16 delay 2 seconds @14:20:15.18 CSV.dial :air @14:20:15.63 delay 2 seconds @14:20:17.64 CSV.dial CTV @14:20:18.10 delay 2 seconds @14:20:20.11 CSV.dial :air @14:20:20.57 delay 2 seconds @14:20:22.59 CS.seek 0ml @14:20:36.85 Puck @27.1psia after final lysis pressurization @14:20:36.88 Recovering Lysate into Collection syringe @14:20:36.89 CSV.dial CBV @14:20:37.91 CBV.dial CSV @14:20:38.30 CS.seek 5ml @14:20:51.96 delay 5 seconds @14:20:56.97 CTV.dial :air @14:20:57.50 CS.seek 7ml @14:21:03.42 delay 5 seconds @14:21:08.44 CSV.dial :air2 @14:21:08.88 CS.seek 10ml @14:21:17.36 CSV.dial between air and CTV,avoiding: CBV @14:21:18.63 Lysing Complete @14:21:18.70 Storing sh1 puck in Collection Clamp to tube 3 @14:21:18.87 SC.reconfigure :tight @14:21:18.92 SC.seek 3 @14:21:22.56 SC.configure :limp @14:21:22.77 Elbow.reconfigure :tight @14:21:22.82 Elbow.seek 3 @14:21:24.50 Forearm.reconfigure :tight @14:21:24.55 Forearm.seek 3 + 800 counts @14:21:25.62 SE.reconfigure :pick @14:21:25.67 SE.seek :beamBreak @14:21:27.98 SE.configure :default @14:21:28.14 Elbow.reconfigure :limp @14:21:28.18 Forearm.reconfigure :loose @14:21:28.23 Forearm.seek 3 @14:21:28.62 Elbow.hold @14:21:28.68 Elbow.reconfigure :tight @14:21:28.78 SE.seek 7.1 Pucks below Top Plate @14:21:29.25 Forearm.force -20 @14:21:29.30 Forearm.seek 3 + 309 counts @14:21:29.58 Forearm.reconfigure :tight @14:21:29.63 Forearm.seek 3 + 800 counts @14:21:29.89 SE.seek 8.1 Pucks below Top Plate @14:21:30.91 Forearm.reconfigure :loose @14:21:30.96 Forearm.seek :retract @14:21:31.86 Elbow.seek CC @14:21:33.45 Elbow.reconfigure :loose @14:21:33.55 Forearm.seek CC @14:21:34.50 Hand.close @14:21:35.23 CC.seek :open @14:21:39.43 Forearm.seek :retract @14:21:40.39 Elbow.reconfigure :tight @14:21:40.44 Elbow.seek 3 @14:21:42.15 Elbow.reconfigure :limp @14:21:42.20 Forearm.seek 3 @14:21:43.35 Elbow.hold @14:21:43.42 Elbow.reconfigure :tight @14:21:43.47 Hand.open @14:21:44.36 Forearm.force -20 @14:21:44.39 Forearm.seek 3 + 318 counts @14:21:44.68 Forearm.reconfigure :tight @14:21:44.72 Forearm.seek 3 + 800 counts @14:21:45.08 SE.seek :down @14:21:46.21
Forearm.reconfigure :loose @14:21:46.26 Forearm.seek :retract @14:21:47.02 SE.stop @14:21:47.19
Tranferring DA @14:21:47.20 Beginning samplePassC @14:21:47.21 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:21:47.40 Elbow.seek FlushPuck::Garage @14:21:49.18 Elbow.reconfigure :loose @14:21:49.29 Forearm.seek :garage @14:21:50.66 Hand.close @14:21:51.48 Forearm.seek :retract @14:21:52.48 Elbow.reconfigure :tight @14:21:52.53 Elbow.seek CC @14:21:55.38 Elbow.reconfigure :loose @14:21:55.48 Forearm.reconfigure :tight @14:21:55.52 Forearm.seek CC @14:21:56.86 CC.seek :closed @14:22:00.86 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.96 @14:22:00.93 Hand.open @14:22:01.67 Forearm.reconfigure :loose @14:22:01.71 Forearm.seek :retract @14:22:02.71 PSV.dial PSR @14:22:04.11 Pulling 1.5ml of PSR.flush PSR.select :flush @14:22:04.24 PS.seek 1.5ml @14:22:20.35 delay 5 seconds @14:22:25.37 PSV.dial :waste1 @14:22:25.84 PS.seek 0ml @14:22:42.03 PSV.dial PSR @14:22:42.50 PSR.select :air @14:22:42.62 PS.seek 2.5ml @14:23:09.04 PRV.dial CBV,avoiding: :ab2 @14:23:09.06
CBV.dial PRV @14:23:10.85 PSV.dial :mixloop @14:23:11.90 CTV.dial :air,avoiding: :intake @14:23:11.97 PTV.dial :PRVmixing @14:23:13.27 PS.seek 0ml @14:23:40.04 PSV.dial :air @14:23:40.49 PS.seek 2.5ml @14:24:06.82 PSV.dial :mixloop @14:24:07.29 PS.seek 0ml @14:24:33.63 PSV.dial :air @14:24:34.10 PS.seek 2.5ml @14:25:00.50 PSV.dial :mixloop @14:25:00.98 PS.seek 0ml @14:25:27.32 Garaging FlushPuck in Collection Clamp @14:25:27.39 Elbow.reconfigure :tight @14:25:27.44 Elbow.seek CC @14:25:27.55 Elbow.reconfigure :loose @14:25:27.65 Forearm.seek CC @14:25:28.60 Hand.close @14:25:29.36 CC.seek :open @14:25:33.45 Forearm.seek :retract @14:25:34.42 Elbow.reconfigure :tight @14:25:34.46 Elbow.seek :garage @14:25:37.31 Elbow.reconfigure :loose @14:25:37.41 Forearm.reconfigure :tight @14:25:37.46 Forearm.seek :garage @14:25:38.61 Hand.open @14:25:39.41 Forearm.reconfigure :loose @14:25:39.46 Forearm.seek :retract @14:25:40.60 PSV.dial :mixloop @14:25:40.77 PTV.dial :PRVmixing @14:25:40.78 PRV.dial CBV @14:25:40.82
CBV.dial PRV @14:25:40.99 CTV.dial :air,avoiding: :intake @14:25:41.00 Loading lyfil puck from tube 4 into Collection Clamp @14:25:41.24 SC.reconfigure :tight @14:25:41.29 SC.seek 4 @14:25:44.91 SC.configure :limp @14:25:45.56 Elbow.reconfigure :tight @14:25:45.61 Elbow.seek 4 @14:25:47.55 Forearm.reconfigure :tight @14:25:47.59 Forearm.seek 4 + 800 counts @14:25:48.82 SE.reconfigure :pick @14:25:48.87 SE.seek :beamBreak @14:25:50.81 SE.configure :default @14:25:50.97 Elbow.seek 4 @14:25:51.22 Forearm.seek 4 + 300 counts @14:25:51.48 Forearm.reconfigure :loose @14:25:51.52 Hand.close @14:25:52.24 SE.seek :down @14:25:52.45
Forearm.seek :retract @14:25:53.49 Elbow.seek CC @14:25:53.91 SE.stop @14:25:55.11
Elbow.reconfigure :loose @14:25:55.21 Forearm.reconfigure :tight @14:25:55.26 Forearm.seek CC @14:25:56.59 CC.seek :closed @14:26:00.61 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.98 @14:26:00.62 Hand.open @14:26:01.41 Forearm.reconfigure :loose @14:26:01.46 Forearm.seek :retract @14:26:02.91 CTV.dial CSV,avoiding: :intake @14:26:04.28 CSV.dial CTV @14:26:04.58 PS.seek 2.5ml @14:26:31.00 CS.seek 4.999ml @14:26:44.64 delay 8 seconds @14:26:52.66 PSV.dial :air @14:26:53.11 delay 2 seconds @14:26:55.13 PSV.dial :waste2 @14:26:55.88 PS.seek 0ml @14:27:22.19 CBV.dial between PRV and waste1 @14:27:22.53 CSV.dial :air @14:27:23.00 CS.seek 10ml @14:27:36.57 CSV.dial CTV @14:27:37.03 CS.seek 0ml @14:28:03.51 CBV.dial :waste1 @14:28:03.84 delay 5 seconds @14:28:08.86 CTV.dial :air,avoiding: :intake @14:28:10.29 Storing lyfil puck in Collection Clamp to tube 3 @14:28:10.47 SC.reconfigure :tight @14:28:10.51 SC.seek 3 @14:28:14.18 SC.configure :limp @14:28:14.39 Elbow.reconfigure :tight @14:28:14.44 Elbow.seek 3 @14:28:16.13 Forearm.reconfigure :tight @14:28:16.18 Forearm.seek 3 + 800 counts @14:28:17.24 SE.reconfigure :pick @14:28:17.29 SE.seek :beamBreak @14:28:19.67 SE.configure :default @14:28:19.82 Elbow.reconfigure :limp @14:28:19.87 Forearm.reconfigure :loose @14:28:19.92 Forearm.seek 3 @14:28:20.29 Elbow.hold @14:28:20.35 Elbow.reconfigure :tight @14:28:20.89 SE.seek 8.1 Pucks below Top Plate @14:28:21.36 Forearm.force -20 @14:28:21.41 Forearm.seek 3 + 318 counts @14:28:21.77 Forearm.reconfigure :tight @14:28:21.81 Forearm.seek 3 + 800 counts @14:28:22.04 SE.seek 9.1 Pucks below Top Plate @14:28:22.64 Forearm.reconfigure :loose @14:28:22.68 Forearm.seek :retract @14:28:23.62 Elbow.seek CC @14:28:25.22 Elbow.reconfigure :loose @14:28:25.32 Forearm.seek CC @14:28:26.25 Hand.close @14:28:26.98 CC.seek :open @14:28:31.18 Forearm.seek :retract @14:28:32.27 Elbow.reconfigure :tight @14:28:32.32 Elbow.seek 3 @14:28:34.04 Elbow.reconfigure :limp @14:28:34.09 Forearm.seek 3 @14:28:35.16 Elbow.hold @14:28:35.22 Elbow.reconfigure :tight @14:28:35.27 Hand.open @14:28:36.10 Forearm.force -20 @14:28:36.15 Forearm.seek 3 + 319 counts @14:28:36.44 Forearm.reconfigure :tight @14:28:36.49 Forearm.seek 3 + 800 counts @14:28:37.28 SE.seek :down @14:28:38.42
Forearm.reconfigure :loose @14:28:38.47 Forearm.seek :retract @14:28:39.14 SE.stop @14:28:39.36
PSV.dial :air @14:28:40.12 PS.seek 2.5ml @14:29:06.50 delay 1 second @14:29:07.51 PSV.dial :mixloop @14:29:07.97 delay 1 second @14:29:08.98 PSV.dial :air @14:29:09.45 delay 1 second @14:29:10.47 PSV.dial :mixloop @14:29:10.94 delay 1 second @14:29:11.95 PSV.dial :air @14:29:12.42 delay 1 second @14:29:13.44 PS.seek 0ml @14:29:39.78 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:29:39.96 Elbow.seek FlushPuck::Garage @14:29:41.75 Elbow.reconfigure :loose @14:29:41.85 Forearm.seek :garage @14:29:42.85 Hand.close @14:29:43.72 Forearm.seek :retract @14:29:44.71 Elbow.reconfigure :tight @14:29:44.76 Elbow.seek PC @14:29:46.20 Elbow.reconfigure :loose @14:29:46.30 Forearm.reconfigure :tight @14:29:46.34 Forearm.seek PC @14:29:47.74 PC.seek :closed @14:29:52.70 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.53 @14:29:52.77 Hand.open @14:29:53.53 Forearm.reconfigure :loose @14:29:53.58 Forearm.seek :retract @14:29:54.66 PRV.dial :air @14:29:55.13 PSV.dial :mixloop @14:29:55.66 PS.seek 0.3ml @14:29:59.50 PSV.dial PSR @14:30:00.55 Pulling 0.4ml of PSR.air @14:30:00.56 PSR.select :air @14:30:00.59 PS.seek 0.7ml @14:30:05.47 delay 3 seconds @14:30:08.48 PSR.select :air @14:30:08.51 PS.seek 2.5ml @14:30:27.69 delay 3 seconds @14:30:30.70 PSV.dial :mixloop @14:30:31.75 PTV.dial :mixing @14:30:32.48 PBV.dial :waste2 @14:30:33.47 PS.seek 0ml @14:30:59.91 Garaging FlushPuck in Processing Clamp @14:30:59.98 Elbow.reconfigure :tight @14:31:00.02 Elbow.seek PC @14:31:00.15 Elbow.reconfigure :loose @14:31:00.25 Forearm.seek PC @14:31:01.35 Hand.close @14:31:02.10 PC.seek :open @14:31:06.61 Forearm.seek :retract @14:31:07.66 Elbow.reconfigure :tight @14:31:07.71 Elbow.seek :garage @14:31:09.01 Elbow.reconfigure :loose @14:31:09.11 Forearm.reconfigure :tight @14:31:09.16 Forearm.seek :garage @14:31:10.28 Hand.open @14:31:11.03 Forearm.reconfigure :loose @14:31:11.08 Forearm.seek :retract @14:31:12.23 Loading da puck from tube 4 into Processing Clamp @14:31:12.46 SC.reconfigure :tight @14:31:12.93 SC.seek 4 @14:31:16.54 SC.configure :limp @14:31:16.76 Elbow.reconfigure :tight @14:31:16.80 Elbow.seek 4 @14:31:18.65 Forearm.reconfigure :tight @14:31:18.70 Forearm.seek 4 + 800 counts @14:31:19.77 SE.reconfigure :pick @14:31:19.82 SE.seek :beamBreak @14:31:21.81 SE.configure :default @14:31:21.96 Elbow.seek 4 @14:31:22.15 Forearm.seek 4 + 300 counts @14:31:22.33 Forearm.reconfigure :loose @14:31:22.38 Hand.close @14:31:23.11 SE.seek :down @14:31:23.31
Forearm.seek :retract @14:31:24.41 Elbow.seek PC @14:31:24.82 SE.stop @14:31:26.98
Elbow.reconfigure :loose @14:31:27.08 Forearm.reconfigure :tight @14:31:27.13 Forearm.seek PC @14:31:28.46 PC.seek :closed @14:31:32.88 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.56 @14:31:32.89 Hand.open @14:31:33.66 Forearm.reconfigure :loose @14:31:34.09 Forearm.seek :retract @14:31:35.17 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @14:31:35.19 PH.seek 28C,5:00 @14:32:23.74 PH.hold 2:00:00,28C @14:32:23.79 delay 1:30 @14:33:53.83 PSR.select :air @14:33:53.84 PSV.dial PSR @14:33:54.90 Pulling 0.5ml of PSR.wash @14:33:54.91 PSR.select :wash @14:33:55.03 PS.seek 0.5ml @14:34:01.00 delay 5 seconds @14:34:06.01 PSR.select :air @14:34:06.14 PS.seek 2ml @14:34:22.28 PTV.dial :puck @14:34:23.03 PSV.dial PTV @14:34:23.79 PBV.dial :waste1 @14:34:25.25 PS.seek 1.5ml @14:34:31.15 delay 3 seconds @14:34:34.17 PBV.dial between PSV and waste1 @14:34:34.55 PS.seek 0ml @14:34:50.68 delay 5:00 @14:39:50.71 recoverPSRreagent08 @14:39:50.72 PTV.dial :puck @14:39:50.79 PBV.dial :waste1 @14:39:51.18 delay 30 seconds @14:40:21.21 PBV.dial between PSV and waste1 @14:40:21.59 PSR.select :air @14:40:21.60 PSV.dial PSR @14:40:22.36 PS.reconfigure :fast @14:40:22.40 PS.seek 2.5ml @14:40:46.12 PSV.dial PTV @14:40:46.87 delay 5 seconds @14:40:51.89 PSV.dial PSR @14:40:52.63 delay 5 seconds @14:40:57.65 PSV.dial PTV @14:40:58.41 delay 5 seconds @14:41:03.43 PSV.dial :air @14:41:04.22 PS.seek 0ml @14:41:27.46 PTV.dial :PRVmixing,via: PRV @14:41:28.77 PSV.dial :mixloop @14:41:29.24 PRV.dial :ab1 @14:41:30.00 PS.reconfigure :slow @14:41:30.04 PS.seek 0.15ml @14:41:33.58 delay 2 seconds @14:41:35.60 CBV.dial PRV @14:41:35.63
PRV.dial CBV @14:41:36.22 PS.seek 0.34ml @14:41:40.52 delay 2 seconds @14:41:42.54 CBV.dial between PRV and PSV @14:41:42.86 PRV.dial :air @14:41:43.31 PS.seek 0.9ml @14:41:55.18 PRV.dial :waste2 @14:41:55.94 PS.seek 0.5ml @14:42:04.68 addPMLReagent08 @14:42:04.69 PSV.dial :air @14:42:05.15 PS.reconfigure :default @14:42:05.20 PS.seek 2ml @14:42:21.43 PSV.dial :PML @14:42:21.90 PTV.dial :mixing @14:42:22.65 PBV.dial :waste1 @14:42:23.52 PS.seek 1.85ml @14:42:25.83 PBV.dial between PSV and waste1 @14:42:26.22 PS.seek 0ml @14:42:46.11 delay 2 seconds @14:42:48.13 Beginning samplePassC @14:42:48.15 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:42:48.33 Elbow.reconfigure :tight @14:42:48.37 Elbow.seek FlushPuck::Garage @14:42:49.67 Elbow.reconfigure :loose @14:42:49.77 Forearm.seek :garage @14:42:50.82 Hand.close @14:42:51.71 Forearm.seek :retract @14:42:52.71 Elbow.reconfigure :tight @14:42:52.76 Elbow.seek CC @14:42:55.55 Elbow.reconfigure :loose @14:42:55.65 Forearm.reconfigure :tight @14:42:55.69 Forearm.seek CC @14:42:57.20 CC.seek :closed @14:43:01.22 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.08 @14:43:01.29 Hand.open @14:43:02.03 Forearm.reconfigure :loose @14:43:02.08 Forearm.seek :retract @14:43:03.01 PSV.dial PSR @14:43:04.07 Pulling 1.5ml of PSR.flush @14:43:04.08 PSR.select :flush @14:43:04.19 PS.seek 1.5ml @14:43:20.30 delay 5 seconds @14:43:25.70 PSV.dial :waste1 @14:43:26.23 PS.seek 0ml @14:43:42.33 PSV.dial PSR @14:43:42.80 PSR.select :air @14:43:42.92 PS.seek 2.5ml @14:44:09.31 PRV.dial CBV,avoiding: :ab2 @14:44:09.33
CBV.dial PRV @14:44:10.99 PSV.dial :mixloop @14:44:12.04 CTV.dial :air,avoiding: :intake @14:44:12.11 PTV.dial :PRVmixing @14:44:12.85 PS.seek 0ml @14:44:39.24 PSV.dial :air @14:44:39.71 PS.seek 2.5ml @14:45:06.11 PSV.dial :mixloop @14:45:06.59 PS.seek 0ml @14:45:32.92 PSV.dial :air @14:45:33.40 PS.seek 2.5ml @14:45:59.80 PSV.dial :mixloop @14:46:00.28 PS.seek 0ml @14:46:26.71 Garaging FlushPuck in Collection Clamp @14:46:26.78 Elbow.reconfigure :tight @14:46:26.82 Elbow.seek CC @14:46:26.94 Elbow.reconfigure :loose @14:46:27.04 Forearm.seek CC @14:46:28.05 Hand.close @14:46:28.84 CC.seek :open @14:46:32.93 Forearm.seek :retract @14:46:33.97 Elbow.reconfigure :tight @14:46:34.02 Elbow.seek :garage @14:46:36.87 Elbow.reconfigure :loose @14:46:36.97 Forearm.reconfigure :tight @14:46:37.02 Forearm.seek :garage @14:46:38.07 Hand.open @14:46:38.89 Forearm.reconfigure :loose @14:46:38.94 Forearm.seek :retract @14:46:39.99 delayUntil "14:50:48.12" @14:46:40.02 Whole Cell Archive sampling 1000ml @14:46:40.47 Loading wcr puck from tube 4 into Collection Clamp @14:46:40.71 SC.reconfigure :tight @14:46:40.76 SC.seek 4 @14:46:40.83 SC.configure :limp @14:46:41.05 Elbow.reconfigure :tight @14:46:41.10 Elbow.seek 4 @14:46:43.05 Forearm.reconfigure :tight @14:46:43.11 Forearm.seek 4 + 800 counts @14:46:44.14 SE.reconfigure :pick @14:46:44.20 SE.seek :beamBreak @14:46:46.24 SE.configure :default @14:46:46.41 Elbow.seek 4 @14:46:46.70 Forearm.seek 4 + 300 counts @14:46:46.99 Forearm.reconfigure :loose @14:46:47.04 Hand.close @14:46:47.84 SE.seek :down @14:46:48.04 Forearm.seek :retract @14:46:49.01 Elbow.seek CC @14:46:49.68 SE.stop @14:46:50.59 Elbow.reconfigure :loose @14:46:50.70 Forearm.reconfigure :tight @14:46:50.75 Forearm.seek CC @14:46:52.44 CC.seek :closed @14:46:56.51 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.94 @14:46:56.53 Hand.open @14:46:57.27 Forearm.reconfigure :loose @14:46:57.32 Forearm.seek :retract @14:46:58.39 collectSample 1000ml w/bubble point of 35psi @14:46:58.41 Priming 7ml @14:46:58.42 Intake.select :close @14:46:58.43 Exhaust.select :close @14:46:58.44 SS.hold @14:46:58.66 Calibrating pressure sensors @14:46:58.67 CTV.dial :air @14:46:58.75 SSV.dial :spit,avoiding: :exhaust @14:46:58.77 SS.reconfigure :fast @14:46:58.82 SS.seek 5ml @14:47:01.49 SSV.dial CBV @14:47:01.52 CBV.dial SSV @14:47:02.91 delay 3.8 seconds @14:47:06.84 Sensor limits: Intake max 98.2psia; Outlet max 108.2psia @14:47:06.85 SSV.dial :spit @14:47:08.21 SS.seek 0ml @14:47:11.28 SSV.dial CBV @14:47:12.64 CTV.dial :intake @14:47:13.42 SSV.dial CBV @14:47:13.45 CBV.dial SSV @14:47:13.60 Allowing 40s for 7.0ml of remaining 7.0ml @14:47:13.61 Intake.select :open @14:47:15.77 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:47:15.79 SS.reconfigure :inhaling 2.17ml/s @14:47:15.84 SS.seek 7ml @14:47:21.09 Stroke completed -- waiting for pressure to equalize ... @14:47:21.10 SS.reconfigure :exhale @14:47:21.15 SS.hold @14:47:24.89 Intake.select :close @14:47:25.05 SSV.dial :exhaust @14:47:25.84 Exhaust.select :open @14:47:25.96 SS.seek 0ml @14:47:30.28 Exhaust.select :close @14:47:30.40 Sampling 1000.0ml @14:47:30.41 Intake.select :close @14:47:30.42 Exhaust.select :close @14:47:30.43 SS.reconfigure :default @14:47:30.48 SS.hold @14:47:30.69 CTV.dial :intake @14:47:30.86 SSV.dial CBV @14:47:30.88 CBV.dial SSV @14:47:31.70 Allowing 130s for 25.0ml of remaining 1000.0ml @14:47:31.72 Intake.select :open @14:47:33.88 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:47:33.90 SS.reconfigure :inhaling 2.17ml/s @14:47:33.95 SS.seek 25ml @14:47:45.85 Vacuum too high -- waiting up to 40 seconds @14:47:45.87 SS.reconfigure :exhale @14:47:45.92 SS.hold @14:47:48.29 Intake @11.5psia, vacuum from 6.0 to 9.0psi @14:47:48.31 SS.reconfigure :inhaling 1.95ml/s @14:47:48.35 SS.seek 25ml @14:47:48.80 Vacuum too high -- waiting up to 40 seconds @14:47:48.81 SS.reconfigure :exhale @14:47:48.87 SS.hold @14:47:59.22 Intake @11.8psia, vacuum from 6.3 to 9.3psi @14:47:59.24 SS.reconfigure :inhaling 1.77ml/s @14:47:59.29 SS.seek 25ml @14:48:03.03 Stroke completed -- waiting for pressure to equalize ... @14:48:03.04 SS.reconfigure :exhale @14:48:03.09 SS.hold @14:48:07.84 Intake.select :close @14:48:07.99 SSV.dial :exhaust @14:48:08.78 Exhaust.select :open @14:48:08.89 SS.seek 0ml @14:48:18.28 Exhaust.select :close @14:48:18.39 SSV.dial CBV @14:48:18.41 CBV.dial SSV @14:48:19.22 Allowing 130s for 25.0ml of remaining 975.0ml @14:48:19.23 Intake.select :open @14:48:21.40 Intake @13.9psia, vacuum from 8.4 to 11.4psi @14:48:21.42 SS.reconfigure :inhaling 1.77ml/s @14:48:21.47 SS.seek 25ml @14:48:26.41 Vacuum too high -- waiting up to 40 seconds @14:48:26.43 SS.reconfigure :exhale @14:48:26.48 SS.hold @14:48:27.65 Intake @11.3psia, vacuum from 5.8 to 8.8psi @14:48:27.67 SS.reconfigure :inhaling 1.59ml/s @14:48:27.72 SS.seek 25ml @14:48:30.41 Vacuum too high -- waiting up to 40 seconds @14:48:30.43 SS.reconfigure :exhale @14:48:30.48 SS.hold @14:48:33.78 SS.reconfigure :inhaling 1.43ml/s @14:48:33.83 SS.seek 25ml @14:48:38.14 Vacuum too high -- waiting up to 40 seconds @14:48:38.16 SS.reconfigure :exhale @14:48:38.21 SS.hold @14:48:40.66 SS.reconfigure :inhaling 1.29ml/s @14:48:40.71 SS.seek 25ml @14:48:44.94 Vacuum too high -- waiting up to 40 seconds @14:48:44.96 SS.reconfigure :exhale @14:48:45.01 SS.hold @14:48:47.61 Intake @11.1psia, vacuum from 5.6 to 8.6psi @14:48:47.63 SS.reconfigure :inhaling 1.16ml/s @14:48:47.68 SS.seek 25ml @14:48:52.38 Vacuum too high -- waiting up to 40 seconds @14:48:52.40 SS.reconfigure :exhale @14:48:52.45 SS.hold @14:48:56.21 Intake @11.9psia, vacuum from 6.4 to 9.4psi @14:48:56.23 SS.reconfigure :inhaling 1.04ml/s @14:48:56.28 SS.seek 25ml @14:48:58.03 Stroke completed -- waiting for pressure to equalize ... @14:48:58.04 SS.reconfigure :exhale @14:48:58.09 SS.hold @14:49:00.57 Intake.select :close @14:49:00.72 SSV.dial :exhaust @14:49:01.48 Exhaust.select :open @14:49:01.60 SS.seek 0ml @14:49:10.84 Exhaust.select :close @14:49:10.95 SSV.dial CBV @14:49:10.98 CBV.dial SSV @14:49:11.80 Allowing 130s for 25.0ml of remaining 950.0ml @14:49:11.81 Intake.select :open @14:49:13.97 Intake @13.2psia, vacuum from 7.7 to 10.7psi @14:49:13.99 SS.reconfigure :inhaling 1.04ml/s @14:49:14.04 SS.seek 25ml @14:49:22.47 Vacuum too high -- waiting up to 40 seconds @14:49:22.49 SS.reconfigure :exhale @14:49:22.54 SS.hold @14:49:24.83 Intake @12.6psia, vacuum from 7.1 to 10.1psi @14:49:24.85 SS.reconfigure :inhaling 0.94ml/s @14:49:24.89 SS.seek 25ml @14:49:35.00 Vacuum too high -- waiting up to 40 seconds @14:49:35.02 SS.reconfigure :exhale @14:49:35.07 SS.hold @14:49:37.77 Intake @11.6psia, vacuum from 6.1 to 9.1psi @14:49:37.79 SS.reconfigure :inhaling 0.85ml/s @14:49:37.84 SS.seek 25ml @14:49:48.37 Stroke completed -- waiting for pressure to equalize ... @14:49:48.38 SS.reconfigure :exhale @14:49:48.43 SS.hold @14:49:54.07 Intake.select :close @14:49:54.22 SSV.dial :exhaust @14:49:55.01 Exhaust.select :open @14:49:55.53 SS.seek 0ml @14:50:04.84 Exhaust.select :close @14:50:04.95 SSV.dial CBV @14:50:04.98 CBV.dial SSV @14:50:05.79 Allowing 130s for 25.0ml of remaining 925.0ml @14:50:05.81 Intake.select :open @14:50:07.98 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:50:07.99 SS.reconfigure :inhaling 0.85ml/s @14:50:08.04 SS.seek 25ml @14:50:26.82 Vacuum too high -- waiting up to 40 seconds @14:50:26.83 SS.reconfigure :exhale @14:50:26.89 SS.hold @14:50:27.32 Intake @11.2psia, vacuum from 5.7 to 8.7psi @14:50:27.34 SS.reconfigure :inhaling 0.77ml/s @14:50:27.39 SS.seek 25ml @14:50:35.82 Vacuum too high -- waiting up to 40 seconds @14:50:35.83 SS.reconfigure :exhale @14:50:35.89 SS.hold @14:50:37.22 Intake @8.6psia, vacuum from 3.1 to 6.1psi @14:50:37.24 SS.reconfigure :inhaling 0.70ml/s @14:50:37.29 SS.seek 25ml @14:50:43.09 Stroke completed -- waiting for pressure to equalize ... @14:50:43.10 SS.reconfigure :exhale @14:50:43.15 SS.hold @14:50:46.69 Intake.select :close @14:50:47.29 SSV.dial :exhaust @14:50:48.07 Exhaust.select :open @14:50:48.14 recoverPRVreagent08 @14:50:48.15 PTV.dial :mixing @14:50:48.20 SS.seek 0ml @14:50:48.89 PBV.dial :waste1 @14:50:49.28 delay 30 seconds @14:50:57.50 Exhaust.select :close @14:50:57.61 SSV.dial CBV @14:50:57.64 CBV.dial SSV @14:50:58.43 Allowing 130s for 25.0ml of remaining 900.0ml @14:50:58.45 Intake.select :open @14:51:00.61 Intake @13.3psia, vacuum from 7.8 to 10.8psi @14:51:00.63 SS.reconfigure :inhaling 0.70ml/s @14:51:00.68 SS.seek 25ml @14:51:19.30 PBV.dial between PSV and waste1 @14:51:19.69 PSV.dial :air @14:51:20.16 PS.reconfigure :fast @14:51:20.21 PS.seek 2.5ml @14:51:37.90 Stroke completed -- waiting for pressure to equalize ... @14:51:37.91 SS.reconfigure :exhale @14:51:37.96 SS.hold @14:51:42.82 Intake.select :close @14:51:42.98 SSV.dial :exhaust @14:51:43.76 Exhaust.select :open @14:51:43.87 SS.seek 0ml @14:51:43.94 PSV.dial :mixloop @14:51:44.41 delay 2 seconds @14:51:46.43 PSV.dial :air @14:51:46.92 delay 2 seconds @14:51:48.94 PSV.dial :mixloop @14:51:49.41 delay 2 seconds @14:51:51.42 PSV.dial :air @14:51:51.90 PS.seek 0ml @14:51:53.21 Exhaust.select :close @14:51:53.33 SSV.dial CBV @14:51:53.36 CBV.dial SSV @14:51:54.16 Allowing 130s for 25.0ml of remaining 875.0ml @14:51:54.17 Intake.select :open @14:51:56.33 Intake @13.4psia, vacuum from 7.9 to 10.9psi @14:51:56.35 SS.reconfigure :inhaling 0.70ml/s @14:51:56.40 SS.seek 25ml @14:52:15.70 washCycleDA #1 of 2 @14:52:15.71 PSR.select :air @14:52:15.73 PSV.dial PSR @14:52:17.09 Pulling 0.5ml of PSR.wash @14:52:17.10 PSR.select :wash @14:52:17.22 PS.reconfigure :default @14:52:17.27 PS.seek 0.5ml @14:52:23.14 delay 5 seconds @14:52:28.16 PSR.select :air @14:52:28.28 PS.seek 2ml @14:52:33.91 Stroke completed -- waiting for pressure to equalize ... @14:52:33.93 SS.reconfigure :exhale @14:52:33.98 SS.hold @14:52:39.64 Intake.select :close @14:52:39.80 SSV.dial :exhaust @14:52:40.59 Exhaust.select :open @14:52:40.70 SS.seek 0ml @14:52:44.83 PTV.dial :puck @14:52:45.67 PSV.dial PTV @14:52:46.44 PBV.dial :waste1 @14:52:46.89 PS.seek 1.5ml @14:52:50.00 Exhaust.select :close @14:52:50.12 SSV.dial CBV @14:52:50.15 CBV.dial SSV @14:52:50.96 Allowing 130s for 25.0ml of remaining 850.0ml @14:52:50.98 Intake.select :open @14:52:52.80 delay 3 seconds @14:52:53.15 Intake @13.9psia, vacuum from 8.4 to 11.4psi @14:52:53.17 SS.reconfigure :inhaling 0.70ml/s @14:52:53.22 SS.seek 25ml @14:52:55.82 PBV.dial between PSV and waste1 @14:52:56.22 PS.seek 0ml @14:53:12.36 delay 2:00 @14:53:30.30 Stroke completed -- waiting for pressure to equalize ... @14:53:30.32 SS.reconfigure :exhale @14:53:30.36 SS.hold @14:53:36.85 Intake.select :close @14:53:37.00 SSV.dial :exhaust @14:53:37.81 Exhaust.select :open @14:53:37.92 SS.seek 0ml @14:53:47.41 Exhaust.select :close @14:53:47.98 SSV.dial CBV @14:53:48.03 CBV.dial SSV @14:53:48.79 Allowing 130s for 25.0ml of remaining 825.0ml @14:53:48.80 Intake.select :open @14:53:50.97 Intake @14.4psia, vacuum from 8.9 to 11.9psi @14:53:50.98 SS.reconfigure :inhaling 0.70ml/s @14:53:51.03 SS.seek 25ml @14:54:28.15 Stroke completed -- waiting for pressure to equalize ... @14:54:28.16 SS.reconfigure :exhale @14:54:28.21 SS.hold @14:54:35.71 Intake.select :close @14:54:35.87 SSV.dial :exhaust @14:54:36.65 Exhaust.select :open @14:54:36.77 SS.seek 0ml @14:54:45.94 Exhaust.select :close @14:54:46.06 SSV.dial CBV @14:54:46.08 CBV.dial SSV @14:54:46.88 Allowing 130s for 25.0ml of remaining 800.0ml @14:54:46.90 Intake.select :open @14:54:49.06 Intake @14.4psia, vacuum from 8.9 to 11.9psi @14:54:49.08 SS.reconfigure :inhaling 0.70ml/s @14:54:49.13 SS.seek 25ml @14:55:12.38 recoverPSRreagent08 @14:55:12.39 PTV.dial :puck @14:55:12.92 PBV.dial :waste1 @14:55:13.31 delay 30 seconds @14:55:26.32 Stroke completed -- waiting for pressure to equalize ... @14:55:26.33 SS.reconfigure :exhale @14:55:26.38 SS.hold @14:55:35.01 Intake.select :close @14:55:35.16 SSV.dial :exhaust @14:55:35.94 Exhaust.select :open @14:55:36.06 SS.seek 0ml @14:55:43.33 PBV.dial between PSV and waste1 @14:55:43.71 PSR.select :air @14:55:43.73 PSV.dial PSR @14:55:44.50 PS.reconfigure :fast @14:55:44.55 PS.seek 2.5ml @14:55:45.40 Exhaust.select :close @14:55:45.51 SSV.dial CBV @14:55:45.54 CBV.dial SSV @14:55:46.33 Allowing 130s for 25.0ml of remaining 775.0ml @14:55:46.35 Intake.select :open @14:55:48.52 Intake @14.3psia, vacuum from 8.8 to 11.8psi @14:55:48.54 SS.reconfigure :inhaling 0.70ml/s @14:55:48.59 SS.seek 25ml @14:56:08.98 PSV.dial PTV @14:56:09.75 delay 5 seconds @14:56:14.77 PSV.dial PSR @14:56:15.54 delay 5 seconds @14:56:15.84 Vacuum too high -- waiting up to 40 seconds @14:56:15.86 SS.reconfigure :exhale @14:56:15.91 SS.hold @14:56:16.34 Intake @11.6psia, vacuum from 6.1 to 9.1psi @14:56:16.36 SS.reconfigure :inhaling 0.62ml/s @14:56:16.41 SS.seek 25ml @14:56:20.56 PSV.dial PTV @14:56:21.33 delay 5 seconds @14:56:26.35 PSV.dial :air @14:56:27.14 PS.seek 0ml @14:56:27.63 Stroke completed -- waiting for pressure to equalize ... @14:56:27.64 SS.reconfigure :exhale @14:56:27.69 SS.hold @14:56:34.19 Intake.select :close @14:56:34.35 SSV.dial :exhaust @14:56:35.13 Exhaust.select :open @14:56:35.24 SS.seek 0ml @14:56:44.19 Exhaust.select :close @14:56:44.30 SSV.dial CBV @14:56:44.34 CBV.dial SSV @14:56:45.57 Allowing 130s for 25.0ml of remaining 750.0ml @14:56:45.58 Intake.select :open @14:56:47.74 Intake @14.4psia, vacuum from 8.9 to 11.9psi @14:56:47.76 SS.reconfigure :inhaling 0.62ml/s @14:56:47.81 SS.seek 25ml @14:56:50.89 washCycleDA #2 of 2 @14:56:50.90 PSR.select :air @14:56:50.92 PSV.dial PSR @14:56:52.28 Pulling 0.5ml of PSR.wash @14:56:52.29 PSR.select :wash @14:56:52.41 PS.reconfigure :default @14:56:52.45 PS.seek 0.5ml @14:56:58.45 delay 5 seconds @14:57:03.47 PSR.select :air @14:57:03.59 PS.seek 2ml @14:57:19.70 PTV.dial :puck @14:57:19.78 PSV.dial PTV @14:57:20.56 PBV.dial :waste1 @14:57:20.99 PS.seek 1.5ml @14:57:26.92 delay 3 seconds @14:57:29.11 Stroke completed -- waiting for pressure to equalize ... @14:57:29.12 SS.reconfigure :exhale @14:57:29.17 SS.hold @14:57:29.93 PBV.dial between PSV and waste1 @14:57:30.33 PS.seek 0ml @14:57:39.48 Intake.select :close @14:57:39.64 SSV.dial :exhaust @14:57:40.43 Exhaust.select :open @14:57:40.54 SS.seek 0ml @14:57:46.54 delay 2:00 @14:57:49.89 Exhaust.select :close @14:57:50.01 SSV.dial CBV @14:57:50.03 CBV.dial SSV @14:57:50.85 Allowing 130s for 25.0ml of remaining 725.0ml @14:57:50.86 Intake.select :open @14:57:53.03 Intake @14.4psia, vacuum from 8.9 to 11.9psi @14:57:53.05 SS.reconfigure :inhaling 0.62ml/s @14:57:53.10 SS.seek 25ml @14:58:06.46 Vacuum too high -- waiting up to 40 seconds @14:58:06.48 SS.reconfigure :exhale @14:58:06.53 SS.hold @14:58:11.63 Intake @13.8psia, vacuum from 8.3 to 11.3psi @14:58:11.65 SS.reconfigure :inhaling 0.56ml/s @14:58:11.70 SS.seek 25ml @14:58:24.78 Vacuum too high -- waiting up to 40 seconds @14:58:24.79 SS.reconfigure :exhale @14:58:24.85 SS.hold @14:58:32.65 Intake @13.7psia, vacuum from 8.2 to 11.2psi @14:58:32.67 SS.reconfigure :inhaling 0.51ml/s @14:58:32.72 SS.seek 25ml @14:58:51.46 Vacuum too high -- waiting up to 40 seconds @14:58:51.48 SS.reconfigure :exhale @14:58:51.53 SS.hold @14:59:01.33 Intake @13.9psia, vacuum from 8.4 to 11.4psi @14:59:01.34 SS.reconfigure :inhaling 0.46ml/s @14:59:01.39 SS.seek 25ml @14:59:03.19 Stroke completed -- waiting for pressure to equalize ... @14:59:03.20 SS.reconfigure :exhale @14:59:03.25 SS.hold @14:59:10.74 Intake.select :close @14:59:10.89 SSV.dial :exhaust @14:59:11.69 Exhaust.select :open @14:59:11.80 SS.seek 0ml @14:59:21.14 Exhaust.select :close @14:59:21.25 SSV.dial CBV @14:59:21.29 CBV.dial SSV @14:59:22.06 Allowing 130s for 25.0ml of remaining 700.0ml @14:59:22.08 Intake.select :open @14:59:24.24 Intake @14.6psia, vacuum from 9.1 to 12.1psi @14:59:24.26 SS.reconfigure :inhaling 0.46ml/s @14:59:24.31 SS.seek 25ml @14:59:46.57 recoverPSRreagent08 @14:59:46.58 PTV.dial :puck @14:59:47.12 PBV.dial :waste1 @14:59:47.51 delay 30 seconds @15:00:17.54 PBV.dial between PSV and waste1 @15:00:17.93 PSR.select :air @15:00:17.95 PSV.dial PSR @15:00:18.72 PS.reconfigure :fast @15:00:18.77 PS.seek 2.5ml @15:00:19.42 Stroke completed -- waiting for pressure to equalize ... @15:00:19.43 SS.reconfigure :exhale @15:00:19.48 SS.hold @15:00:25.99 Intake.select :close @15:00:26.14 SSV.dial :exhaust @15:00:26.94 Exhaust.select :open @15:00:27.06 SS.seek 0ml @15:00:36.39 Exhaust.select :close @15:00:36.50 SSV.dial CBV @15:00:36.54 CBV.dial SSV @15:00:37.31 Allowing 130s for 25.0ml of remaining 675.0ml @15:00:37.33 Intake.select :open @15:00:39.50 Intake @14.9psia, vacuum from 9.4 to 12.4psi @15:00:39.51 SS.reconfigure :inhaling 0.46ml/s @15:00:39.56 SS.seek 25ml @15:00:42.82 PSV.dial PTV @15:00:43.59 delay 5 seconds @15:00:48.61 PSV.dial PSR @15:00:49.37 delay 5 seconds @15:00:54.39 PSV.dial PTV @15:00:55.60 delay 5 seconds @15:01:00.62 PSV.dial :air @15:01:01.41 PS.seek 0ml @15:01:24.95 getPMLReagent08 @15:01:24.96 PRV.dial :air @15:01:25.43 delay 5 seconds @15:01:30.45 PTV.dial :PRVmixing @15:01:31.81 delay 5 seconds @15:01:34.79 Stroke completed -- waiting for pressure to equalize ... @15:01:34.81 SS.reconfigure :exhale @15:01:34.85 SS.hold @15:01:36.88 PRV.dial :ab2 @15:01:37.35 PTV.dial :PRVmixing @15:01:37.43 PSV.dial :mixloop @15:01:37.90 PS.reconfigure :default @15:01:37.95 PS.seek 0.3ml @15:01:41.85 delay 2 seconds @15:01:42.31 Intake.select :close @15:01:42.47 SSV.dial :exhaust @15:01:43.25 Exhaust.select :open @15:01:43.36 SS.seek 0ml @15:01:43.87 PRV.dial :air @15:01:44.40 PS.seek 0.55ml @15:01:47.73 addPMLReagent08 @15:01:47.74 PSV.dial :air @15:01:48.20 PS.seek 2ml @15:01:52.79 Exhaust.select :close @15:01:52.90 SSV.dial CBV @15:01:52.92 CBV.dial SSV @15:01:53.73 Allowing 130s for 25.0ml of remaining 650.0ml @15:01:53.75 Intake.select :open @15:01:55.91 Intake @15.3psia, vacuum from 9.8 to 12.8psi @15:01:55.94 SS.reconfigure :inhaling 0.46ml/s @15:01:55.98 SS.seek 25ml @15:02:03.82 PSV.dial :PML @15:02:04.34 PTV.dial :mixing @15:02:05.09 PBV.dial :waste1 @15:02:05.54 PS.seek 1.85ml @15:02:07.91 PBV.dial between PSV and waste1 @15:02:08.31 PS.seek 0ml @15:02:28.07 delay 2 seconds @15:02:30.09 delay 8:00 @15:02:51.43 Stroke completed -- waiting for pressure to equalize ... @15:02:51.45 SS.reconfigure :exhale @15:02:51.50 SS.hold @15:02:58.95 Intake.select :close @15:02:59.10 SSV.dial :exhaust @15:02:59.89 Exhaust.select :open @15:03:00.01 SS.seek 0ml @15:03:09.27 Exhaust.select :close @15:03:09.38 SSV.dial CBV @15:03:09.42 CBV.dial SSV @15:03:10.19 Allowing 130s for 25.0ml of remaining 625.0ml @15:03:10.20 Intake.select :open @15:03:12.37 Intake @15.2psia, vacuum from 9.7 to 12.7psi @15:03:12.39 SS.reconfigure :inhaling 0.46ml/s @15:03:12.44 SS.seek 25ml @15:04:07.81 Stroke completed -- waiting for pressure to equalize ... @15:04:07.82 SS.reconfigure :exhale @15:04:07.87 SS.hold @15:04:14.37 Intake.select :close @15:04:14.53 SSV.dial :exhaust @15:04:15.31 Exhaust.select :open @15:04:15.42 SS.seek 0ml @15:04:24.79 Exhaust.select :close @15:04:24.91 SSV.dial CBV @15:04:24.93 CBV.dial SSV @15:04:25.75 Allowing 130s for 25.0ml of remaining 600.0ml @15:04:25.76 Intake.select :open @15:04:27.92 Intake @15.6psia, vacuum from 10.0 to 13.0psi @15:04:27.94 SS.reconfigure :inhaling 0.46ml/s @15:04:27.99 SS.seek 25ml @15:05:23.09 Stroke completed -- waiting for pressure to equalize ... @15:05:23.10 SS.reconfigure :exhale @15:05:23.55 SS.hold @15:05:30.93 Intake.select :close @15:05:31.08 SSV.dial :exhaust @15:05:31.87 Exhaust.select :open @15:05:31.98 SS.seek 0ml @15:05:41.40 Exhaust.select :close @15:05:41.51 SSV.dial CBV @15:05:41.55 CBV.dial SSV @15:05:42.34 Allowing 130s for 25.0ml of remaining 575.0ml @15:05:42.35 Intake.select :open @15:05:44.51 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:05:44.53 SS.reconfigure :inhaling 0.46ml/s @15:05:44.58 SS.seek 25ml @15:06:39.69 Stroke completed -- waiting for pressure to equalize ... @15:06:39.71 SS.reconfigure :exhale @15:06:39.75 SS.hold @15:06:47.52 Intake.select :close @15:06:47.68 SSV.dial :exhaust @15:06:48.46 Exhaust.select :open @15:06:48.58 SS.seek 0ml @15:06:57.95 Exhaust.select :close @15:06:58.06 SSV.dial CBV @15:06:58.09 CBV.dial SSV @15:06:58.90 Allowing 130s for 25.0ml of remaining 550.0ml @15:06:58.91 Intake.select :open @15:07:01.08 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:07:01.09 SS.reconfigure :inhaling 0.46ml/s @15:07:01.14 SS.seek 25ml @15:07:56.41 Stroke completed -- waiting for pressure to equalize ... @15:07:56.42 SS.reconfigure :exhale @15:07:56.47 SS.hold @15:08:06.27 Intake.select :close @15:08:06.43 SSV.dial :exhaust @15:08:07.22 Exhaust.select :open @15:08:07.33 SS.seek 0ml @15:08:16.84 Exhaust.select :close @15:08:16.95 SSV.dial CBV @15:08:16.99 CBV.dial SSV @15:08:17.76 Allowing 130s for 25.0ml of remaining 525.0ml @15:08:17.77 Intake.select :open @15:08:19.94 Intake @15.9psia, vacuum from 10.0 to 13.0psi @15:08:19.96 SS.reconfigure :inhaling 0.46ml/s @15:08:20.01 SS.seek 25ml @15:09:15.41 Stroke completed -- waiting for pressure to equalize ... @15:09:15.42 SS.reconfigure :exhale @15:09:15.47 SS.hold @15:09:28.16 Intake.select :close @15:09:28.31 SSV.dial :exhaust @15:09:29.10 Exhaust.select :open @15:09:29.21 SS.seek 0ml @15:09:38.63 Exhaust.select :close @15:09:38.74 SSV.dial CBV @15:09:38.77 CBV.dial SSV @15:09:39.58 Allowing 130s for 25.0ml of remaining 500.0ml @15:09:39.60 Intake.select :open @15:09:41.77 Intake @16.0psia, vacuum from 10.0 to 13.0psi @15:09:41.78 SS.reconfigure :inhaling 0.46ml/s @15:09:41.83 SS.seek 25ml @15:10:30.11 recoverPRVreagent08 @15:10:30.12 PTV.dial :mixing @15:10:30.28 PBV.dial :waste1 @15:10:30.67 delay 30 seconds @15:10:31.89 Vacuum too high -- waiting up to 40 seconds @15:10:31.91 SS.reconfigure :exhale @15:10:31.96 SS.hold @15:10:38.55 Intake @15.1psia, vacuum from 9.6 to 12.6psi @15:10:38.57 SS.reconfigure :inhaling 0.42ml/s @15:10:38.62 SS.seek 25ml @15:10:44.63 Stroke completed -- waiting for pressure to equalize ... @15:10:44.64 SS.reconfigure :exhale @15:10:44.69 SS.hold @15:10:59.08 Intake.select :close @15:10:59.69 SSV.dial :exhaust @15:11:00.49 Exhaust.select :open @15:11:00.60 SS.seek 0ml @15:11:00.69 PBV.dial between PSV and waste1 @15:11:01.08 PSV.dial :air @15:11:01.55 PS.reconfigure :fast @15:11:01.60 PS.seek 2.5ml @15:11:09.96 Exhaust.select :close @15:11:10.07 SSV.dial CBV @15:11:10.11 CBV.dial SSV @15:11:10.88 Allowing 130s for 25.0ml of remaining 475.0ml @15:11:10.89 Intake.select :open @15:11:13.05 Intake @16.2psia, vacuum from 10.0 to 13.0psi @15:11:13.07 SS.reconfigure :inhaling 0.42ml/s @15:11:13.12 SS.seek 25ml @15:11:25.72 PSV.dial :mixloop @15:11:26.19 delay 2 seconds @15:11:28.21 PSV.dial :air @15:11:28.67 delay 2 seconds @15:11:30.69 PSV.dial :mixloop @15:11:31.16 delay 2 seconds @15:11:33.18 PSV.dial :air @15:11:33.65 PS.seek 0ml @15:11:57.22 washCycleDA #1 of 3 @15:11:57.23 PSR.select :air @15:11:57.25 PSV.dial PSR @15:11:57.98 Vacuum too high -- waiting up to 40 seconds @15:11:58.00 SS.reconfigure :exhale @15:11:58.05 SS.hold @15:11:58.64 Pulling 0.5ml of PSR.wash @15:11:58.68 PSR.select :wash @15:11:58.86 PS.reconfigure :default @15:11:58.93 PS.seek 0.5ml @15:12:03.53 Intake @15.5psia, vacuum from 10.0 to 13.0psi @15:12:03.62 SS.reconfigure :inhaling 0.38ml/s @15:12:03.67 SS.seek 25ml @15:12:04.94 delay 5 seconds @15:12:09.96 PSR.select :air @15:12:10.07 PS.seek 2ml @15:12:21.63 Stroke completed -- waiting for pressure to equalize ... @15:12:21.64 SS.reconfigure :exhale @15:12:21.69 SS.hold @15:12:26.15 PTV.dial :puck @15:12:26.92 PSV.dial PTV @15:12:27.67 PBV.dial :waste1 @15:12:28.12 PS.seek 1.5ml @15:12:34.41 delay 3 seconds @15:12:34.49 Intake.select :close @15:12:34.65 SSV.dial :exhaust @15:12:35.44 Exhaust.select :open @15:12:35.55 SS.seek 0ml @15:12:37.44 PBV.dial between PSV and waste1 @15:12:37.84 PS.seek 0ml @15:12:45.45 Exhaust.select :close @15:12:45.57 SSV.dial CBV @15:12:45.61 CBV.dial SSV @15:12:46.39 Allowing 130s for 25.0ml of remaining 450.0ml @15:12:46.41 Intake.select :open @15:12:48.57 Intake @16.4psia, vacuum from 10.0 to 13.0psi @15:12:48.59 SS.reconfigure :inhaling 0.38ml/s @15:12:48.64 SS.seek 25ml @15:12:54.00 delay 2:00 @15:13:56.53 Stroke completed -- waiting for pressure to equalize ... @15:13:56.54 SS.reconfigure :exhale @15:13:56.59 SS.hold @15:14:11.01 Intake.select :close @15:14:11.64 SSV.dial :exhaust @15:14:12.42 Exhaust.select :open @15:14:12.54 SS.seek 0ml @15:14:21.97 Exhaust.select :close @15:14:22.08 SSV.dial CBV @15:14:22.10 CBV.dial SSV @15:14:22.91 Allowing 130s for 25.0ml of remaining 425.0ml @15:14:22.92 Intake.select :open @15:14:25.09 Intake @16.5psia, vacuum from 10.0 to 13.0psi @15:14:25.11 SS.reconfigure :inhaling 0.38ml/s @15:14:25.15 SS.seek 25ml @15:14:54.03 recoverPSRreagent08 @15:14:54.04 PTV.dial :puck @15:14:54.11 PBV.dial :waste1 @15:14:54.48 delay 30 seconds @15:15:24.51 PBV.dial between PSV and waste1 @15:15:24.91 PSR.select :air @15:15:24.93 PSV.dial PSR @15:15:25.69 PS.reconfigure :fast @15:15:25.74 PS.seek 2.5ml @15:15:33.04 Stroke completed -- waiting for pressure to equalize ... @15:15:33.06 SS.reconfigure :exhale @15:15:33.10 SS.hold @15:15:45.82 Intake.select :close @15:15:45.98 SSV.dial :exhaust @15:15:46.76 Exhaust.select :open @15:15:46.88 SS.seek 0ml @15:15:49.63 PSV.dial PTV @15:15:50.39 delay 5 seconds @15:15:55.41 PSV.dial PSR @15:15:56.29 delay 5 seconds @15:15:56.32 Exhaust.select :close @15:15:56.43 SSV.dial CBV @15:15:56.45 CBV.dial SSV @15:15:57.26 Allowing 130s for 25.0ml of remaining 400.0ml @15:15:57.28 Intake.select :open @15:15:59.45 Intake @16.7psia, vacuum from 10.0 to 13.0psi @15:15:59.46 SS.reconfigure :inhaling 0.38ml/s @15:15:59.51 SS.seek 25ml @15:16:01.30 PSV.dial PTV @15:16:02.05 delay 5 seconds @15:16:07.07 PSV.dial :air @15:16:07.85 PS.seek 0ml @15:16:31.44 washCycleDA #2 of 3 @15:16:31.45 PSR.select :air @15:16:31.47 PSV.dial PSR @15:16:32.83 Pulling 0.5ml of PSR.wash @15:16:32.84 PSR.select :wash @15:16:32.95 PS.reconfigure :default @15:16:33.00 PS.seek 0.5ml @15:16:38.94 delay 5 seconds @15:16:43.96 PSR.select :air @15:16:44.08 PS.seek 2ml @15:17:00.25 PTV.dial :puck @15:17:00.26 PSV.dial PTV @15:17:01.03 PBV.dial :waste1 @15:17:01.48 PS.seek 1.5ml @15:17:07.40 delay 3 seconds @15:17:07.62 Stroke completed -- waiting for pressure to equalize ... @15:17:07.63 SS.reconfigure :exhale @15:17:07.68 SS.hold @15:17:10.42 PBV.dial between PSV and waste1 @15:17:10.80 PS.seek 0ml @15:17:27.00 delay 2:00 @15:17:48.35 SS.reconfigure :default @15:17:48.41 SS.hold @15:17:48.48 Intake.select :close @15:17:48.64 ShallowSampler::Clogged in WCR -- Intake @16psia & Outlet @14psia won't equalize after 625.0ml @15:17:48.65 SSV.dial :exhaust @15:17:49.43 Exhaust.select :open @15:17:49.55 SS.reconfigure :exhale @15:17:49.60 SS.seek 0ml @15:17:58.56 Exhaust.select :close @15:17:58.67 Sampled 625.0ml @15:17:58.69 Drying puck before fixing for 4:00 @15:17:58.70 CBV.dial :waste2b @15:17:59.46 delay 1:00 @15:18:59.49 CSV.dial :air @15:18:59.95 CS.reconfigure :fast @15:19:00.00 CS.seek 10ml @15:19:24.35 Pumping wcr puck from tube 4 up to 35psi... @15:19:24.37 CBV.dial SSV @15:19:25.63 CBV.dial between CSV and waste2b @15:19:26.62 CSV.dial between air and CTV @15:19:26.95 CS.seek 6.407ml @15:19:27.02 recoverPSRreagent08 @15:19:27.03 PTV.dial :puck @15:19:27.04 PBV.dial :waste1 @15:19:27.43 delay 30 seconds @15:19:36.13 CTV.dial CSV,avoiding: :air @15:19:36.16 CSV.dial CTV @15:19:37.02 CS.reconfigure :pressurize @15:19:37.08 CS.seek 0ml @15:19:51.59 CBV.dial :waste2b @15:19:51.93 CS.reconfigure :default @15:19:51.98 CS.seek 0ml @15:19:55.13 delay 1:00 @15:19:57.45 PBV.dial between PSV and waste1 @15:19:57.84 PSR.select :air @15:19:57.86 PSV.dial PSR @15:19:58.63 PS.reconfigure :fast @15:19:58.67 PS.seek 2.5ml @15:20:22.37 PSV.dial PTV @15:20:23.14 delay 5 seconds @15:20:28.16 PSV.dial PSR @15:20:28.90 delay 5 seconds @15:20:33.92 PSV.dial PTV @15:20:34.68 delay 5 seconds @15:20:39.70 PSV.dial :air @15:20:40.50 PS.seek 0ml @15:20:55.15 CTV.dial :air,avoiding: :intake @15:20:57.02 Fix #1 of 1 (with RNAlater) for 4:00 @15:20:57.04 CSV.dial CSR @15:20:57.50 Pulling 2ml of CSR.RNAlater @15:20:57.51 CSR.select :RNAlater @15:20:57.63 CS.seek 2ml @15:21:03.56 delay 5 seconds @15:21:04.12 washCycleDA #3 of 3 @15:21:04.13 PSR.select :air @15:21:04.15 PSV.dial PSR @15:21:05.49 Pulling 0.5ml of PSR.wash @15:21:05.51 PSR.select :wash @15:21:05.62 PS.reconfigure :default @15:21:05.67 PS.seek 0.5ml @15:21:08.58 CSR.select :air @15:21:08.71 CS.reconfigure :fast @15:21:08.76 CS.seek 10ml @15:21:11.59 delay 5 seconds @15:21:16.61 PSR.select :air @15:21:16.72 PS.seek 2ml @15:21:28.32 Pumping wcr puck from tube 4 up to 35psi... @15:21:28.33 CBV.dial SSV @15:21:29.59 CBV.dial between CSV and waste2b @15:21:30.57 CSV.dial between air and CTV @15:21:31.20 CS.seek 6.3ml @15:21:32.77 PTV.dial :puck @15:21:32.79 PSV.dial PTV @15:21:33.55 PBV.dial :waste1 @15:21:33.99 PS.seek 1.5ml @15:21:39.96 delay 3 seconds @15:21:40.76 CTV.dial CSV,avoiding: :air @15:21:40.78 CSV.dial CTV @15:21:42.23 CS.reconfigure :pressurize @15:21:42.28 CS.seek 0ml @15:21:42.98 PBV.dial between PSV and waste1 @15:21:43.38 PS.seek 0ml @15:21:55.27 delay 4:00 @15:21:59.50 delay 2:00 @15:23:59.53 recoverPSRreagent08 PTV.dial :puck @15:23:59.54 PBV.dial :waste1 @15:23:59.93 delay 30 seconds @15:24:29.95 PBV.dial between PSV and waste1 @15:24:30.34 PSR.select :air @15:24:30.36 PSV.dial PSR @15:24:31.12 PS.reconfigure :fast @15:24:31.17 PS.seek 2.5ml @15:24:54.84 PSV.dial PTV @15:24:55.60 delay 5 seconds @15:25:00.62 PSV.dial PSR @15:25:01.38 delay 5 seconds @15:25:06.40 PSV.dial PTV @15:25:07.18 delay 5 seconds @15:25:12.20 PSV.dial :air @15:25:12.98 PS.seek 0ml @15:25:36.43 Gate.power :camera,:ON @15:25:36.44 Mixing DA Substrates @15:25:36.45 PSR.select :air @15:25:36.47 PSV.dial PSR @15:25:37.87 Pulling 0.25ml of PSR.sub1 @15:25:37.89 PSR.select :sub1 @15:25:38.08 PS.reconfigure :default @15:25:38.20 PS.seek 0.25ml @15:25:41.60 delay 5 seconds @15:25:46.62 Pulling 0.25ml of PSR.sub2 @15:25:46.64 PSR.select :sub2 @15:25:46.75 PS.seek 0.5ml @15:25:50.15 delay 5 seconds @15:25:55.17 PSR.select :air @15:25:55.28 PS.seek 2ml @15:25:55.33 CBV.dial :waste2b @15:25:55.67 CS.reconfigure :default @15:25:55.72 CS.seek 0ml @15:26:00.26 delay 1:00 @15:26:11.46 PTV.dial :puck @15:26:11.47 PSV.dial PTV @15:26:12.24 PBV.dial :waste1 @15:26:12.69 PS.seek 1.5ml @15:26:18.65 delay 3 seconds @15:26:21.67 PBV.dial between PSV and waste1 @15:26:22.06 PS.seek 0ml @15:26:38.24 delay 10 seconds @15:26:48.27 recoverPSRreagent08 @15:26:48.28 PTV.dial :puck @15:26:48.29 PBV.dial :waste1 @15:26:48.68 delay 30 seconds @15:27:00.28 CTV.dial :air,avoiding: :intake @15:27:18.70 PBV.dial between PSV and waste1 @15:27:19.10 PSR.select :air @15:27:19.12 PSV.dial PSR @15:27:19.89 PS.reconfigure :fast @15:27:19.93 PS.seek 2.5ml @15:27:43.87 PSV.dial PTV @15:27:44.63 delay 5 seconds @15:27:49.65 PSV.dial PSR @15:27:50.43 delay 5 seconds @15:27:55.45 PSV.dial PTV @15:27:56.21 delay 5 seconds @15:28:01.23 PSV.dial :air @15:28:02.03 PS.seek 0ml @15:28:25.68 Additional air clear @15:28:25.69 PSR.select :air @15:28:25.71 PSV.dial PSR @15:28:27.07 Pulling 2ml of PSR.air @15:28:27.08 PSR.select :air @15:28:27.10 PS.reconfigure :default @15:28:27.15 PS.seek 2ml @15:28:48.36 delay 5 seconds @15:28:53.39 PTV.dial :puck @15:28:53.41 PSV.dial PTV @15:28:54.16 PBV.dial :waste1 @15:28:54.61 PS.seek 1.5ml @15:29:00.96 delay 3 seconds @15:29:03.98 PBV.dial between PSV and waste1 @15:29:04.37 delay 1 second @15:29:05.38 PSV.dial :waste1 @15:29:06.46 PS.seek 0ml @15:29:23.12 Processing Puck Heater at 28C before imaging @15:29:23.13 PH.coast @15:29:23.24 Imaging da puck in Processing Clamp while storing it to tube 3 @15:29:23.42 SC.reconfigure :tight @15:29:23.47 SC.seek 3 @15:29:27.10 SC.configure :limp @15:29:27.31 Elbow.reconfigure :tight @15:29:27.36 Elbow.seek 3 @15:29:29.05 Forearm.reconfigure :tight @15:29:29.10 Forearm.seek 3 + 800 counts @15:29:30.18 SE.reconfigure :pick @15:29:30.24 SE.seek :beamBreak @15:29:32.35 SE.configure :default @15:29:32.51 Elbow.reconfigure :limp @15:29:32.56 Forearm.reconfigure :loose @15:29:32.61 Forearm.seek 3 @15:29:33.02 Elbow.hold @15:29:33.09 Elbow.reconfigure :tight @15:29:33.19 SE.seek 9.1 Pucks below Top Plate @15:29:33.68 Forearm.force -20 @15:29:33.72 Forearm.seek 3 + 314 counts @15:29:34.01 Forearm.reconfigure :tight @15:29:34.06 Forearm.seek 3 + 800 counts @15:29:34.42 SE.seek 10.1 Pucks below Top Plate @15:29:34.98 Forearm.reconfigure :loose @15:29:35.03 Forearm.seek :retract @15:29:35.93 Elbow.seek PC @15:29:38.48 Elbow.reconfigure :loose @15:29:38.58 Forearm.seek PC @15:29:39.72 Hand.close @15:29:40.42 PC.seek :open @15:29:45.47 Forearm.seek :retract @15:29:46.54 Elbow.reconfigure :tight @15:29:46.59 Elbow.seek 3 @15:29:49.20 Forearm.reconfigure :tight @15:29:49.25 Forearm.seek 3 + 800 counts @15:29:50.27 Hand.open @15:29:51.10 SE.seek 9.86 Pucks below Top Plate @15:29:51.37 SE.reconfigure :seal @15:29:51.42 SE.seek 9.2 Pucks below Top Plate @15:29:51.93 Lights, camera, ACTION! @15:29:51.97 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da25nov2515h275ml.tif @15:30:11.47 Waiting >20 seconds for da to relinquish FlushPuck @15:30:15.89 SX HX9/16 (USB:13): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 20.64 seconds /var/log/esp/da25nov2515h275ml.tif: TIFF Upload Complete @15:30:15.92 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da25nov2515h275ml15s.tif @15:30:31.56 SX HX9/16 (USB:13): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da25nov2515h275ml15s.tif: TIFF Upload Complete @15:30:31.59 PuckCamera: snap -bin=2,2 21.0 /var/log/esp/midres/da25nov2515h275ml21s.tif @15:30:53.24 SX HX9/16 (USB:13): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21 seconds /var/log/esp/midres/da25nov2515h275ml21s.tif: TIFF Upload Complete @15:30:53.26 Gate.power :camera,:OFF @15:30:53.28 DAfans.email "20.64 sec auto-exposure",Subject: "da25nov2515h275ml images!" @15:30:53.70 Elbow.seek 3 @15:30:54.10 Forearm.seek 3 + 800 counts @15:30:54.38 SE.reconfigure :gently @15:30:54.45 SE.seek 9.91 Pucks below Top Plate @15:30:55.24 Elbow.reconfigure :limp @15:30:55.30 Forearm.reconfigure :loose @15:30:55.35 Forearm.seek 3 @15:30:55.79 Elbow.hold @15:30:55.86 Elbow.reconfigure :tight @15:30:55.91 SE.reconfigure :default @15:30:55.96 SE.seek 10.1 Pucks below Top Plate @15:30:56.38 Forearm.force -20 @15:30:56.43 Forearm.seek 3 + 300 counts @15:30:56.96 Forearm.reconfigure :tight @15:30:57.09 Forearm.seek 3 + 800 counts @15:30:57.42 SE.seek :down @15:30:58.67 Forearm.reconfigure :loose @15:30:58.77 Forearm.seek :retract @15:30:59.87 SE.stop @15:31:00.12 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:31:00.62 Elbow.seek FlushPuck::Garage @15:31:02.53 Elbow.reconfigure :loose @15:31:02.72 Forearm.seek :garage @15:31:03.83 Hand.close @15:31:04.85 Forearm.seek :retract @15:31:05.90 Elbow.reconfigure :tight @15:31:05.99 Elbow.seek PC @15:31:07.36 Elbow.reconfigure :loose @15:31:07.57 Forearm.reconfigure :tight @15:31:07.66 Forearm.seek PC @15:31:09.46 PC.seek :closed @15:31:14.00 Processing Clamp onSpring/offSpring for 12/26 samples, on/off torque 1.53 @15:31:14.16 Hand.open @15:31:15.12 Forearm.reconfigure :loose @15:31:15.33 Forearm.seek :retract @15:31:16.38 PSR.select :air @15:31:16.44 Storing wcr puck in Collection Clamp to tube 3 @15:31:16.48 PSV.dial PSR @15:31:16.96 SC.reconfigure :tight @15:31:17.06 SC.seek 3 @15:31:17.18 Pulling 0.5ml of PSR.flush @15:31:17.24 PSR.select :flush @15:31:18.41 SC.configure :limp @15:31:18.67 PS.seek 0.5ml @15:31:19.03 Elbow.reconfigure :tight @15:31:19.13 Elbow.seek 3 @15:31:21.95 Forearm.reconfigure :tight @15:31:22.07 Forearm.seek 3 + 800 counts @15:31:23.24 SE.reconfigure :pick @15:31:23.37 SE.seek :beamBreak @15:31:24.77 delay 5 seconds @15:31:24.96 upsync @15:31:25.42 SE.configure :default @15:31:25.58 Elbow.reconfigure :limp @15:31:25.64 Forearm.reconfigure :loose @15:31:25.68 Forearm.seek 3 @15:31:26.08 Elbow.hold @15:31:26.15 Elbow.reconfigure :tight @15:31:26.26 SE.seek 10.11 Pucks below Top Plate @15:31:26.79 Forearm.force -20 @15:31:26.83 Forearm.seek 3 + 289 counts @15:31:27.13 Forearm.reconfigure :tight @15:31:27.18 Forearm.seek 3 + 800 counts @15:31:27.47 SE.seek 11.11 Pucks below Top Plate @15:31:28.03 Forearm.reconfigure :loose @15:31:28.08 Forearm.seek :retract @15:31:29.01 Elbow.seek CC @15:31:29.79 PSR.select :air @15:31:29.90 PS.seek 2.5ml @15:31:30.58 Elbow.reconfigure :loose @15:31:31.07 Forearm.seek CC @15:31:32.07 Hand.close @15:31:32.79 CC.seek :open @15:31:37.01 Forearm.seek :retract @15:31:37.99 Elbow.reconfigure :tight @15:31:38.04 Elbow.seek 3 @15:31:39.74 Elbow.reconfigure :limp @15:31:39.80 Forearm.seek 3 @15:31:40.93 Elbow.hold @15:31:40.99 Elbow.reconfigure :tight @15:31:41.04 Hand.open @15:31:41.92 Forearm.force -20 @15:31:41.96 Forearm.seek 3 + 319 counts @15:31:42.25 Forearm.reconfigure :tight @15:31:42.30 Forearm.seek 3 + 800 counts @15:31:42.71 SE.seek :down @15:31:43.85 Forearm.reconfigure :loose @15:31:43.90 Forearm.seek :retract @15:31:44.36 SE.stop @15:31:44.87
Starting finalPurge @15:31:51.08 delay 1 second @15:31:52.10 PRV.dial :waste2,avoiding: :ab2 @15:31:54.00 PTV.dial :PRVmixing @15:31:55.30 PSV.dial :mixloop @15:31:56.36 PS.seek :empty @15:32:04.41
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:32:22.77 PSR.select :air @15:32:22.79 PSV.dial PSR @15:32:23.85 Pulling 0.5ml of PSR.flush @15:32:23.86 PSR.select :flush @15:32:23.97 PS.seek 0.5ml @15:32:30.00 delay 5 seconds @15:32:35.02 PSR.select :air @15:32:35.15 PS.seek 2.5ml @15:32:56.33 delay 1 second @15:32:57.35 PBV.dial :waste1 @15:32:57.72 PTV.dial :mixing @15:32:58.47 PSV.dial :mixloop @15:32:59.54 PS.seek :empty @15:33:25.86 PSV.dial PSR @15:33:26.91 PSR.select :flush @15:33:27.03 PS.seek 1ml @15:33:38.11 PSR.select :air @15:33:38.23 PS.seek 2.5ml @15:33:54.36 PSV.dial PTV @15:33:55.13 PTV.dial :puck @15:33:55.88 PBV.dial :waste1 @15:33:55.90 PS.seek 0ml @15:34:22.27 PSV.dial PSR @15:34:23.02 PSR.select :flush @15:34:23.14 PS.seek 0.5ml @15:34:29.02 PSR.select :air @15:34:29.13 PS.seek 2.5ml @15:34:50.33 PSV.dial PTV @15:34:51.09 PBV.dial PSV @15:34:51.71 PS.seek 0ml @15:35:18.05 PSV.dial PBV @15:35:18.52 PS.seek 2.5ml @15:35:44.92 PSV.dial :waste1 @15:35:45.68 PS.seek 0ml @15:36:12.05 PSV.dial PSR @15:36:12.51 PSR.select :air @15:36:12.54 PS.seek 2.5ml @15:36:38.89 PSV.dial PTV @15:36:39.67 PBV.dial PSV @15:36:39.87 PS.seek 0ml @15:37:06.23 PSV.dial PBV @15:37:06.71 PS.seek 2.5ml @15:37:33.11 PSV.dial :waste1 @15:37:33.85 PS.seek 0ml @15:38:00.23 PSV.dial PSR @15:38:00.70 PSR.select :flush @15:38:00.82 PS.seek 1ml @15:38:11.82 PSR.select :air @15:38:11.95 PS.seek 2.5ml @15:38:28.23 PSV.dial PTV @15:38:29.35 PTV.dial :puck @15:38:29.48 PBV.dial :waste1 @15:38:30.05 PS.seek 0ml @15:38:56.42 PSV.dial PSR @15:38:57.17 PSR.select :flush @15:38:57.29 PS.seek 0.5ml @15:39:03.29 PSR.select :air @15:39:03.41 PS.seek 2.5ml @15:39:24.70 PSV.dial PTV @15:39:25.45 PBV.dial PSV @15:39:26.02 PS.seek 0ml @15:39:52.45 PSV.dial PBV @15:39:53.03 PS.seek 2.5ml @15:40:19.39 PSV.dial :waste1 @15:40:20.14 PS.seek 0ml @15:40:46.48 PSV.dial PSR @15:40:46.95 PSR.select :air @15:40:46.97 PS.seek 2.5ml @15:41:13.67 PSV.dial PTV @15:41:14.42 PBV.dial PSV @15:41:14.50 PS.seek 0ml @15:41:40.82 PSV.dial PBV @15:41:41.30 PS.seek 2.5ml @15:42:07.57 PSV.dial :waste1 @15:42:08.31 PS.seek 0ml @15:42:34.70
Loading FlushPuck into Collection Clamp from Processing Clamp @15:42:34.89 Elbow.seek PC @15:42:37.38 Elbow.reconfigure :loose @15:42:37.48 Forearm.seek PC @15:42:38.59 Hand.close @15:42:39.28 PC.seek :open @15:42:43.78 Forearm.seek :retract @15:42:44.84 Elbow.reconfigure :tight @15:42:44.89 Elbow.seek CC @15:42:48.46 Elbow.reconfigure :loose @15:42:48.55 Forearm.reconfigure :tight @15:42:48.60 Forearm.seek CC @15:42:50.37 CC.seek :closed @15:42:54.39 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.92 @15:42:54.45 Hand.open @15:42:55.21 Forearm.reconfigure :loose @15:42:55.25 Forearm.seek :retract @15:42:56.23 Flushing the Collection Valve::Manifold @15:42:56.35 CSV.dial CSR @15:42:56.78 Pulling 5ml of CSR.flush @15:42:56.79 CSR.select :flush @15:42:56.91 CS.seek 5ml @15:43:10.58 delay 5 seconds @15:43:15.60 CSR.select :air @15:43:15.77 CS.reconfigure :fast @15:43:15.81 CS.seek 7.5ml @15:43:22.48 CSV.dial :waste @15:43:23.77 CS.reconfigure :default @15:43:23.82 CS.seek 0ml @15:43:43.83 Flushing lower manifold's waste1 port @15:43:43.95 CSV.dial :flush @15:43:44.40 CS.reconfigure :fast @15:43:44.44 CS.seek 5ml @15:43:57.08 delay 5 seconds @15:44:02.09 CSV.dial :air @15:44:02.61 CS.seek 7ml @15:44:08.05 CSV.dial CTV @15:44:08.07
CTV.dial CSV @15:44:09.48 CBV.dial :waste1 @15:44:10.27 CS.seek 0ml @15:44:27.20 Flushing lower manifold's waste2 port @15:44:27.32 CSV.dial :flush @15:44:28.07 CS.seek 5ml @15:44:40.54 delay 5 seconds @15:44:45.56 CSV.dial :air @15:44:46.08 CS.seek 7ml @15:44:51.55 CSV.dial CTV @15:44:51.57
CTV.dial CSV @15:44:52.16 CBV.dial :waste2 @15:44:53.22 CS.seek 0ml @15:45:10.14 Flushing lower manifold's waste2b port @15:45:10.26 CSV.dial :flush @15:45:11.00 CS.seek 5ml @15:45:23.42 delay 5 seconds @15:45:28.44 CSV.dial :air @15:45:28.97 CS.seek 7ml @15:45:34.61 CSV.dial CTV @15:45:34.64
CTV.dial CSV @15:45:35.09 CBV.dial :waste2b @15:45:36.14 CS.seek 0ml @15:45:53.01 Disinfecting external Intake with kill @15:45:53.14 CSV.dial CSR @15:45:53.70 Pulling 7.999ml of CSR.kill @15:45:53.71 CSR.select :kill @15:45:53.83 CS.seek 8ml @15:46:13.29 delay 5 seconds @15:46:18.31 CSR.select :air @15:46:18.44 CS.seek 10ml @15:46:24.01 CSV.dial CBV @15:46:24.04
CBV.dial CSV @15:46:25.12 CTV.dial :intake @15:46:25.90 Intake.select :open @15:46:26.02 CS.seek 2ml @15:46:45.17 Intake.select :close @15:46:45.27 CTV.dial :air @15:46:46.03 CS.reconfigure :default @15:46:46.08 CS.seek 0ml @15:46:51.95 CSV.dial CSR @15:46:52.96 Pulling 5ml of CSR.flush @15:46:52.97 CSR.select :flush @15:46:53.10 CS.seek 5ml @15:47:06.76 delay 5 seconds @15:47:11.78 CSR.select :air @15:47:11.90 CS.seek 10ml @15:47:25.57 CSV.dial CBV @15:47:26.60 CS.seek 0ml @15:47:53.01 Garaging FlushPuck in Collection Clamp @15:47:53.13 Elbow.reconfigure :tight @15:47:53.18 Elbow.seek CC @15:47:53.30 Elbow.reconfigure :loose @15:47:53.39 Forearm.seek CC @15:47:54.35 Hand.close @15:47:55.03 CC.seek :open @15:47:59.50 Forearm.seek :retract @15:48:00.62 Elbow.reconfigure :tight @15:48:00.67 Elbow.seek :garage @15:48:03.52 Elbow.reconfigure :loose @15:48:03.61 Forearm.reconfigure :tight @15:48:03.66 Forearm.seek :garage @15:48:04.73 Hand.open @15:48:05.58 Forearm.reconfigure :loose @15:48:05.63 Forearm.seek :retract @15:48:06.74 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 13:44:37 @15:48:06.79 Asleep after completing DA_WCR_112525 assays @15:48:06.81 GoodNews.email "{1 => 22,\n 2 => 22,\n 3 => 12,\n 4 => 10,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@15:44:01, 42% flow, 18.1C, 28% humidity, 14.5psia, 13.65V, 0.353A avg, 356.485Ah, 4.81W\nSampled 625.0ml",Subject: "Asleep after completing DA_WCR_112525 assays" @15:48:10.81 upsync + ESPwhich DA_WCR_120325.rb + fn=DA_WCR_120325.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_120325.rb + [ -f /home/esp/esp2/mission/DA_WCR_120325.rb ] + realpath /home/esp/esp2/mission/DA_WCR_120325.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_120325.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_120325.rb @17:43:29.16UTC03-Dec-25
ESP2 v3.42 I2C Gateway v3.20 for core bus @17:43:31.88 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @17:43:35.66 Polling Can every 10:00 @17:43:35.79 Cleanly exited at 17:43:13UTC03-Dec-25 @17:43:35.83 Loading /home/esp/esp2local/mission/DA_WCR_120325.rb mission @17:43:35.89 Preparing to sleep until + 00:00:05 @17:43:35.91 GoodNews.email "Starting DA_WCR_120325 w/pucks in tube 4\n{1 => 22,\n 2 => 22,\n 3 => 12,\n 4 => 10,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@17:43:35, 42% flow, 18.6C, 29% humidity, 14.5psia, 13.64V, 0.277A avg, 393.074Ah, 3.78W",Subject: "DA_WCR_120325 begun" @17:43:35.95
Asleep til + 00:00:05 @17:43:35.98 Continuing at 17:43 @17:43:36.23 Gate.power :core,:OFF @17:43:36.24 Gate.power :raw,:OFF @17:43:36.25 Shutdown @17:43:39.12 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120325.rb:14\n{1 => 22,\n 2 => 22,\n 3 => 12,\n 4 => 10,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@17:43:36, 42% flow, 18.6C, 29% humidity, 14.5psia, 13.64V, 0.277A avg, 393.074Ah, 3.78W",Subject: "Asleep until 17:43" @17:43:40.61 upsync @17:43:40.89
Powering core bus @17:43:43.48 upsync @17:43:46.45
Gate.power :raw,:ON @17:43:47.46 CS.configure :default @17:43:47.51 PS.configure :default @17:43:47.55 SS.configure :default @17:43:47.60 CC.configure :default @17:43:47.65 PC.configure :default @17:43:47.71 Forearm.configure :loose @17:43:47.76 Elbow.configure :loose @17:43:47.83 SC.configure :loose @17:43:47.88 SE.configure :default @17:43:47.92 CH.configure :default @17:43:47.96 PH.configure :default @17:43:48.00 All dwarf controllers running firmware version 3.85 @17:43:48.11 Valve.reset @17:43:48.33 Intake.select :close @17:43:48.39 Exhaust.select :close @17:43:48.42 CTV.dial :air @17:43:48.52 PSV.dial :air @17:43:48.55 CSV.dial :waste1 @17:43:48.70
Forearm.home.seek :retract @17:43:49.19 SSV.dial :spit,avoiding: :exhaust @17:43:49.41 Hand.close @17:43:49.44
Elbow.reconfigure :loose @17:43:49.54 Elbow.jog 1500 @17:43:49.66 PS.home.seek 0ml @17:43:50.00
Elbow.reconfigure :loose @17:43:50.05 Elbow.home.seek :clear @17:43:50.13 CS.reconfigure :fast @17:43:50.16 SS.home.seek 0ml @17:43:50.24 CS.home.seek 0ml @17:43:53.23
SE.home.seek :down @17:43:53.98 SC.reconfigure :tight @17:43:54.03 SC.home.seek :initial @17:43:58.72 PS.stop @17:44:09.94
SC.configure :limp @17:44:10.00 Forearm.seek :retract @17:44:10.06 Elbow.seek :hammer @17:44:11.08 Forearm.reconfigure :tight @17:44:11.13 Forearm.seek retracted @17:44:11.95 PC.home.seek :open @17:44:11.97
CC.home.seek :open @17:44:15.11 Notice.email "At 17:44:15 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_120325.rb:14\n{1 => 22,\n 2 => 22,\n 3 => 12,\n 4 => 10,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@17:44:15, 42% flow, 18.5C, 29% humidity, 14.5psia, 13.56V, 0.259A avg, 393.079Ah, 3.51W",Subject: "Started DA_WCR_120325 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @17:44:15.14
Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @17:44:15.16 Starting initialPurge @17:44:15.17 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:44:15.24 Hand.open @17:44:16.09 Elbow.reconfigure :tight @17:44:16.16 Elbow.seek FlushPuck::Garage @17:44:18.02 Elbow.reconfigure :loose @17:44:18.13 Forearm.reconfigure :loose @17:44:18.18 Forearm.seek :garage @17:44:19.09 upsync @17:44:19.16
Hand.close @17:44:20.07 Forearm.seek :retract @17:44:21.07 Elbow.reconfigure :tight @17:44:21.11 Elbow.seek CC @17:44:23.94 Elbow.reconfigure :loose @17:44:24.05 Forearm.reconfigure :tight @17:44:24.09 Forearm.seek CC @17:44:25.39 CC.seek :closed @17:44:29.37 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.93 @17:44:29.43 Hand.open @17:44:30.27 Forearm.reconfigure :loose @17:44:30.32 Forearm.seek :retract @17:44:31.30 Flushing the Collection Valve::Manifold @17:44:31.43 CSV.dial CSR @17:44:32.72 Pulling 5ml of CSR.flush @17:44:32.73 CSR.select :flush @17:44:32.84 CS.reconfigure :default @17:44:32.89 CS.seek 5ml @17:44:46.98 delay 5 seconds @17:44:52.00 CSR.select :air @17:44:52.17 CS.reconfigure :fast @17:44:52.22 CS.seek 7.501ml @17:44:58.89 CSV.dial :waste @17:45:00.19 CS.reconfigure :default @17:45:00.24 CS.seek 0ml @17:45:20.27 Purging external Intake @17:45:20.39 CSV.dial :flush @17:45:20.85 CS.reconfigure :fast @17:45:20.89 CS.seek 10ml @17:45:45.01 delay 5 seconds @17:45:50.03 CSV.dial CBV @17:45:50.08
CBV.dial CSV @17:45:50.79 CTV.dial :intake @17:45:51.59 Intake.select :open @17:45:51.70 CS.seek 0ml @17:46:15.82 Intake.select :close @17:46:15.93 CTV.dial :air @17:46:16.72 Flushing upper manifold @17:46:16.84 CSV.dial :flush @17:46:17.59 CS.seek 5ml @17:46:30.14 delay 5 seconds @17:46:35.16 CSV.dial :air @17:46:35.67 CS.seek 7ml @17:46:41.23 CSV.dial CBV @17:46:41.27
CBV.dial CSV @17:46:42.31 CTV.dial :air @17:46:42.39 CS.seek 0ml @17:46:59.36 delay 7 seconds @17:47:06.38 CS.seek 3ml @17:47:14.17 CSV.dial :waste @17:47:14.63 CS.seek 0ml @17:47:22.56 CSV.dial :flush @17:47:23.00 CS.seek 3ml @17:47:30.82 delay 5 seconds @17:47:35.84 CSV.dial :air @17:47:36.35 CS.seek 5ml @17:47:41.88 CSV.dial :air @17:47:42.08 CS.seek 0ml @17:47:54.45 delay 7 seconds @17:48:01.48 CS.seek 3ml @17:48:09.29 CSV.dial :waste @17:48:10.02 CS.seek 0ml @17:48:17.79 Garaging FlushPuck in Collection Clamp @17:48:17.87 Elbow.reconfigure :tight @17:48:17.91 Elbow.seek CC @17:48:18.02 Elbow.reconfigure :loose @17:48:18.12 Forearm.seek CC @17:48:19.09 Hand.close @17:48:19.83 CC.seek :open @17:48:23.98 Forearm.seek :retract @17:48:25.03 Elbow.reconfigure :tight @17:48:25.08 Elbow.seek :garage @17:48:27.94 Elbow.reconfigure :loose @17:48:28.04 Forearm.reconfigure :tight @17:48:28.09 Forearm.seek :garage @17:48:29.05 Hand.open @17:48:29.88 Forearm.reconfigure :loose @17:48:29.93 Forearm.seek :retract @17:48:30.87 da sampling at most 1000ml, wcr at most 1000ml @17:48:30.92 Loading sh1 puck from tube 4 into Collection Clamp @17:48:30.93 DAfans.email "Started da in tube 4 at 17:48:30UTC03-Dec-25\n{1 => 22,\n 2 => 22,\n 3 => 12,\n 4 => 10,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@17:44:15, 42% flow, 18.5C, 29% humidity, 14.5psia, 13.56V, 0.259A avg, 393.079Ah, 3.51W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @17:48:31.21
SC.reconfigure :tight @17:48:31.25 SC.seek 4 @17:48:31.32 SC.configure :limp @17:48:31.58 Elbow.reconfigure :tight @17:48:31.63 Elbow.seek 4 @17:48:33.50 Forearm.reconfigure :tight @17:48:33.55 Forearm.seek 4 + 800 counts @17:48:34.64 SE.reconfigure :pick @17:48:34.70 SE.seek :beamBreak @17:48:35.10 upsync @17:48:36.81
SE.configure :default @17:48:36.97 Elbow.seek 4 @17:48:37.16 Forearm.seek 4 + 300 counts @17:48:37.57 Forearm.reconfigure :loose @17:48:37.62 Hand.close @17:48:38.34 SE.seek :down @17:48:38.92
Forearm.seek :retract @17:48:39.94 Elbow.seek CC @17:48:40.27 SE.stop @17:48:41.49
Elbow.reconfigure :loose @17:48:41.60 Forearm.reconfigure :tight @17:48:41.64 Forearm.seek CC @17:48:42.91 CC.seek :closed @17:48:46.92 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.13 @17:48:46.93 Hand.open @17:48:47.79 Forearm.reconfigure :loose @17:48:47.83 Forearm.seek :retract @17:48:48.98 collectSample 1000ml w/bubble point of 24psi @17:48:49.00 Priming 7ml @17:48:49.01 Intake.select :close @17:48:49.02 Exhaust.select :close @17:48:49.03 SS.hold @17:48:49.23 Calibrating pressure and temperature sensors @17:48:49.26 CTV.dial :air @17:48:49.28 SSV.dial :spit,avoiding: :exhaust @17:48:49.35 SS.reconfigure :fast @17:48:49.40 SS.seek 5ml @17:48:52.33 SSV.dial CBV @17:48:52.36
CBV.dial SSV @17:48:53.74 delay 3.8 seconds @17:48:57.67 Sensor limits: Intake max 90.1psia; Outlet max 108.1psia @17:48:57.68 SSV.dial :spit @17:48:59.03 SS.seek 0ml @17:49:01.77 SSV.dial CBV @17:49:03.13 CTV.dial :intake @17:49:03.90 SSV.dial CBV @17:49:03.93
CBV.dial SSV @17:49:04.02 Allowing 40s for 7.0ml of remaining 7.0ml @17:49:04.03 Intake.select :open @17:49:06.20 Intake @14.5psia, vacuum from 9.0 to 12.0psi @17:49:06.21 SS.reconfigure :inhaling 2.17ml/s @17:49:06.26 SS.seek 7ml @17:49:12.81 Stroke completed -- waiting for pressure to equalize ... @17:49:12.82 SS.reconfigure :exhale @17:49:12.87 SS.hold @17:49:14.66 Intake.select :close @17:49:14.82 SSV.dial :exhaust @17:49:15.60 Exhaust.select :open @17:49:15.71 SS.seek 0ml @17:49:20.03 Exhaust.select :close @17:49:20.14 Sampling 1000.0ml @17:49:20.15 Intake.select :close @17:49:20.16 Exhaust.select :close @17:49:20.17 SS.reconfigure :default @17:49:20.21 SS.hold @17:49:20.41 CTV.dial :intake @17:49:20.59 SSV.dial CBV @17:49:20.63
CBV.dial SSV @17:49:21.39 Allowing 130s for 25.0ml of remaining 1000.0ml @17:49:21.41 Intake.select :open @17:49:23.57 Intake @14.0psia, vacuum from 8.5 to 11.5psi @17:49:23.59 SS.reconfigure :inhaling 2.17ml/s @17:49:23.64 SS.seek 25ml @17:49:37.89 Stroke completed -- waiting for pressure to equalize ... @17:49:37.90 SS.reconfigure :exhale @17:49:37.95 SS.hold @17:49:46.54 Intake.select :close @17:49:46.69 SSV.dial :exhaust @17:49:47.46 Exhaust.select :open @17:49:47.57 SS.seek 0ml @17:49:56.84 Exhaust.select :close @17:49:56.95 SSV.dial CBV @17:49:56.97
CBV.dial SSV @17:49:57.75 Allowing 130s for 25.0ml of remaining 975.0ml @17:49:57.77 Intake.select :open @17:49:59.93 Intake @13.7psia, vacuum from 8.2 to 11.2psi @17:49:59.94 SS.reconfigure :inhaling 2.17ml/s @17:49:59.99 SS.seek 25ml @17:50:10.48 Vacuum too high -- waiting up to 40 seconds @17:50:10.49 SS.reconfigure :exhale @17:50:10.54 SS.hold @17:50:11.33 Intake @8.8psia, vacuum from 3.3 to 6.3psi @17:50:11.35 SS.reconfigure :inhaling 1.95ml/s @17:50:11.40 SS.seek 25ml @17:50:14.04 Vacuum too high -- waiting up to 40 seconds @17:50:14.05 SS.reconfigure :exhale @17:50:14.11 SS.hold @17:50:20.10 Intake @11.8psia, vacuum from 6.3 to 9.3psi @17:50:20.12 SS.reconfigure :inhaling 1.77ml/s @17:50:20.17 SS.seek 25ml @17:50:23.50 Stroke completed -- waiting for pressure to equalize ... @17:50:23.51 SS.reconfigure :exhale @17:50:23.56 SS.hold @17:50:24.80 Intake.select :close @17:50:24.95 SSV.dial :exhaust @17:50:25.72 Exhaust.select :open @17:50:25.83 SS.seek 0ml @17:50:34.99 Exhaust.select :close @17:50:35.09 SSV.dial CBV @17:50:35.12
CBV.dial SSV @17:50:35.91 Allowing 130s for 25.0ml of remaining 950.0ml @17:50:35.92 Intake.select :open @17:50:38.08 Intake @12.2psia, vacuum from 6.7 to 9.7psi @17:50:38.10 SS.reconfigure :inhaling 1.77ml/s @17:50:38.15 SS.seek 25ml @17:50:49.02 Vacuum too high -- waiting up to 40 seconds @17:50:49.04 SS.reconfigure :exhale @17:50:49.09 SS.hold @17:50:49.65 Intake @7.8psia, vacuum from 2.3 to 5.3psi @17:50:49.66 SS.reconfigure :inhaling 1.59ml/s @17:50:49.71 SS.seek 25ml @17:50:49.82 Vacuum too high -- waiting up to 40 seconds @17:50:49.84 SS.reconfigure :exhale @17:50:50.31 SS.hold @17:50:54.60 Intake @12.0psia, vacuum from 6.5 to 9.5psi @17:50:54.61 SS.reconfigure :inhaling 1.43ml/s @17:50:54.67 SS.seek 25ml @17:51:02.28 Stroke completed -- waiting for pressure to equalize ... @17:51:02.29 SS.reconfigure :exhale @17:51:02.34 SS.hold @17:51:05.30 Intake.select :close @17:51:05.46 SSV.dial :exhaust @17:51:06.22 Exhaust.select :open @17:51:06.33 SS.seek 0ml @17:51:15.59 Exhaust.select :close @17:51:15.70 SSV.dial CBV @17:51:15.72
CBV.dial SSV @17:51:16.50 Allowing 130s for 25.0ml of remaining 925.0ml @17:51:16.51 Intake.select :open @17:51:18.68 Intake @13.4psia, vacuum from 7.9 to 10.9psi @17:51:18.69 SS.reconfigure :inhaling 1.43ml/s @17:51:18.74 SS.seek 25ml @17:51:30.19 Vacuum too high -- waiting up to 40 seconds @17:51:30.21 SS.reconfigure :exhale @17:51:30.26 SS.hold @17:51:30.76 Intake @9.7psia, vacuum from 4.2 to 7.2psi @17:51:30.78 SS.reconfigure :inhaling 1.29ml/s @17:51:30.82 SS.seek 25ml @17:51:36.88 Vacuum too high -- waiting up to 40 seconds @17:51:36.89 SS.reconfigure :exhale @17:51:36.94 SS.hold @17:51:40.85 Intake @11.2psia, vacuum from 5.7 to 8.7psi @17:51:40.86 SS.reconfigure :inhaling 1.16ml/s @17:51:40.91 SS.seek 25ml @17:51:44.83 Stroke completed -- waiting for pressure to equalize ... @17:51:44.84 SS.reconfigure :exhale @17:51:44.88 SS.hold @17:51:46.50 Intake.select :close @17:51:46.65 SSV.dial :exhaust @17:51:47.41 Exhaust.select :open @17:51:47.52 SS.seek 0ml @17:51:56.70 Exhaust.select :close @17:51:56.81 SSV.dial CBV @17:51:56.84
CBV.dial SSV @17:51:57.63 Allowing 130s for 25.0ml of remaining 900.0ml @17:51:57.64 Intake.select :open @17:51:59.80 Intake @13.0psia, vacuum from 7.5 to 10.5psi @17:51:59.82 SS.reconfigure :inhaling 1.16ml/s @17:51:59.87 SS.seek 25ml @17:52:11.48 Vacuum too high -- waiting up to 40 seconds @17:52:11.49 SS.reconfigure :exhale @17:52:11.54 SS.hold @17:52:12.87 Intake @10.3psia, vacuum from 4.8 to 7.8psi @17:52:12.88 SS.reconfigure :inhaling 1.04ml/s @17:52:12.93 SS.seek 25ml @17:52:17.22 Vacuum too high -- waiting up to 40 seconds @17:52:17.24 SS.reconfigure :exhale @17:52:17.29 SS.hold @17:52:19.17 Intake @10.1psia, vacuum from 4.6 to 7.6psi @17:52:19.19 SS.reconfigure :inhaling 0.94ml/s @17:52:19.24 SS.seek 25ml @17:52:29.48 Stroke completed -- waiting for pressure to equalize ... @17:52:29.49 SS.reconfigure :exhale @17:52:29.54 SS.hold @17:52:33.07 Intake.select :close @17:52:33.23 SSV.dial :exhaust @17:52:33.98 Exhaust.select :open @17:52:34.09 SS.seek 0ml @17:52:43.34 Exhaust.select :close @17:52:43.45 SSV.dial CBV @17:52:43.47
CBV.dial SSV @17:52:44.25 Allowing 130s for 25.0ml of remaining 875.0ml @17:52:44.26 Intake.select :open @17:52:46.43 Intake @13.7psia, vacuum from 8.2 to 11.2psi @17:52:46.44 SS.reconfigure :inhaling 0.94ml/s @17:52:46.49 SS.seek 25ml @17:53:03.38 Vacuum too high -- waiting up to 40 seconds @17:53:03.39 SS.reconfigure :exhale @17:53:03.44 SS.hold @17:53:03.87 Intake @10.3psia, vacuum from 4.8 to 7.8psi @17:53:03.89 SS.reconfigure :inhaling 0.85ml/s @17:53:03.94 SS.seek 25ml @17:53:09.94 Vacuum too high -- waiting up to 40 seconds @17:53:09.95 SS.reconfigure :exhale @17:53:10.01 SS.hold @17:53:14.68 Intake @13.6psia, vacuum from 8.1 to 11.1psi @17:53:14.70 SS.reconfigure :inhaling 0.77ml/s @17:53:14.75 SS.seek 25ml @17:53:22.36 Stroke completed -- waiting for pressure to equalize ... @17:53:22.37 SS.reconfigure :exhale @17:53:22.41 SS.hold @17:53:24.80 Intake.select :close @17:53:24.95 SSV.dial :exhaust @17:53:25.72 Exhaust.select :open @17:53:25.83 SS.seek 0ml @17:53:34.62 Exhaust.select :close @17:53:34.73 SSV.dial CBV @17:53:34.77
CBV.dial SSV @17:53:35.53 Allowing 130s for 25.0ml of remaining 850.0ml @17:53:35.54 Intake.select :open @17:53:37.71 Intake @13.8psia, vacuum from 8.3 to 11.3psi @17:53:37.72 SS.reconfigure :inhaling 0.77ml/s @17:53:37.77 SS.seek 25ml @17:54:03.25 Vacuum too high -- waiting up to 40 seconds @17:54:03.27 SS.reconfigure :exhale @17:54:03.32 SS.hold @17:54:05.43 Intake @10.4psia, vacuum from 4.9 to 7.9psi @17:54:05.44 SS.reconfigure :inhaling 0.70ml/s @17:54:05.49 SS.seek 25ml @17:54:15.26 Stroke completed -- waiting for pressure to equalize ... @17:54:15.27 SS.reconfigure :exhale @17:54:15.32 SS.hold @17:54:19.51 Intake.select :close @17:54:19.66 SSV.dial :exhaust @17:54:20.43 Exhaust.select :open @17:54:20.54 SS.seek 0ml @17:54:29.43 Exhaust.select :close @17:54:29.54 SSV.dial CBV @17:54:29.56
CBV.dial SSV @17:54:30.34 Allowing 130s for 25.0ml of remaining 825.0ml @17:54:30.35 Intake.select :open @17:54:32.52 Intake @13.6psia, vacuum from 8.1 to 11.1psi @17:54:32.53 SS.reconfigure :inhaling 0.70ml/s @17:54:32.58 SS.seek 25ml @17:54:45.85 Vacuum too high -- waiting up to 40 seconds @17:54:45.86 SS.reconfigure :exhale @17:54:45.91 SS.hold @17:54:47.24 Intake @11.2psia, vacuum from 5.7 to 8.7psi @17:54:47.26 SS.reconfigure :inhaling 0.62ml/s @17:54:47.30 SS.seek 25ml @17:54:53.94 Vacuum too high -- waiting up to 40 seconds @17:54:53.95 SS.reconfigure :exhale @17:54:54.00 SS.hold @17:54:58.99 Intake @11.1psia, vacuum from 5.6 to 8.6psi @17:54:59.01 SS.reconfigure :inhaling 0.56ml/s @17:54:59.05 SS.seek 25ml @17:55:16.47 Vacuum too high -- waiting up to 40 seconds @17:55:16.48 SS.reconfigure :exhale @17:55:16.54 SS.hold @17:55:22.69 Intake @13.9psia, vacuum from 8.4 to 11.4psi @17:55:22.71 SS.reconfigure :inhaling 0.51ml/s @17:55:22.76 SS.seek 25ml @17:55:28.84 Stroke completed -- waiting for pressure to equalize ... @17:55:28.85 SS.reconfigure :exhale @17:55:28.90 SS.hold @17:55:32.42 Intake.select :close @17:55:32.58 SSV.dial :exhaust @17:55:33.34 Exhaust.select :open @17:55:33.45 SS.seek 0ml @17:55:42.31 Exhaust.select :close @17:55:42.42 SSV.dial CBV @17:55:42.44
CBV.dial SSV @17:55:43.25 Allowing 130s for 25.0ml of remaining 800.0ml @17:55:43.26 Intake.select :open @17:55:45.42 Intake @14.0psia, vacuum from 8.5 to 11.5psi @17:55:45.44 SS.reconfigure :inhaling 0.51ml/s @17:55:45.49 SS.seek 25ml @17:56:01.38 Vacuum too high -- waiting up to 40 seconds @17:56:01.39 SS.reconfigure :exhale @17:56:01.44 SS.hold @17:56:06.55 Intake @13.3psia, vacuum from 7.8 to 10.8psi @17:56:06.57 SS.reconfigure :inhaling 0.46ml/s @17:56:06.61 SS.seek 25ml @17:56:16.06 Vacuum too high -- waiting up to 40 seconds @17:56:16.08 SS.reconfigure :exhale @17:56:16.13 SS.hold @17:56:23.61 SS.reconfigure :inhaling 0.42ml/s @17:56:23.66 SS.seek 25ml @17:56:39.28 Vacuum too high -- waiting up to 40 seconds @17:56:39.29 SS.reconfigure :exhale @17:56:39.35 SS.hold @17:56:46.38 Intake @13.2psia, vacuum from 7.7 to 10.7psi @17:56:46.40 SS.reconfigure :inhaling 0.38ml/s @17:56:46.45 SS.seek 25ml @17:57:04.40 Stroke completed -- waiting for pressure to equalize ... @17:57:04.41 SS.reconfigure :exhale @17:57:04.45 SS.hold @17:57:33.92 Intake.select :close @17:57:34.07 SSV.dial :exhaust @17:57:34.85 Exhaust.select :open @17:57:34.96 SS.seek 0ml @17:57:44.46 Exhaust.select :close @17:57:44.57 SSV.dial CBV @17:57:44.61
CBV.dial SSV @17:57:45.38 Allowing 130s for 25.0ml of remaining 775.0ml @17:57:45.40 Intake.select :open @17:57:47.56 Intake @14.0psia, vacuum from 8.5 to 11.5psi @17:57:47.58 SS.reconfigure :inhaling 0.38ml/s @17:57:47.63 SS.seek 25ml @17:57:59.53 Vacuum too high -- waiting up to 40 seconds @17:57:59.54 SS.reconfigure :exhale @17:57:59.60 SS.hold @17:58:24.22 Intake @14.4psia, vacuum from 8.9 to 11.9psi @17:58:24.24 SS.reconfigure :inhaling 0.33ml/s @17:58:24.72 SS.seek 25ml @17:58:35.28 Vacuum too high -- waiting up to 40 seconds @17:58:35.29 SS.reconfigure :exhale @17:58:35.35 SS.hold @17:59:06.13 Intake @14.6psia, vacuum from 9.1 to 12.1psi @17:59:06.15 SS.reconfigure :inhaling 0.30ml/s @17:59:06.19 SS.seek 25ml @17:59:19.68 Vacuum too high -- waiting up to 40 seconds @17:59:19.70 SS.reconfigure :exhale @17:59:19.75 SS.hold @17:59:55.41 SS.reconfigure :default @17:59:55.45 SS.hold @17:59:55.51 Intake.select :close @17:59:55.66 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 236.4ml @17:59:55.68 SSV.dial :exhaust @17:59:56.46 Exhaust.select :open @17:59:56.57 SS.reconfigure :exhale @17:59:56.62 SS.seek 0ml @18:00:02.46 Exhaust.select :close @18:00:02.57 Sampled 236.4ml @18:00:02.58 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @18:00:02.59 Intake.select :close @18:00:02.65 CSV.dial :waste @18:00:03.26 SSV.dial CBV,avoiding: :exhaust @18:00:03.29 CS.seek 0ml @18:00:03.32
CBV.dial SSV @18:00:03.42 CSV.dial :air @18:00:04.15 CS.seek 10ml @18:00:05.26
CTV.dial :air @18:00:06.03 delay 15 seconds @18:00:21.06 SSV.dial :spit,avoiding: :exhaust @18:00:22.42 CTV.dial CSV,avoiding: :intake @18:00:22.45
SS.seek 0ml @18:00:22.57 SSV.dial CBV,avoiding: :exhaust @18:00:28.57 SSV.dial between CBV and plug @18:00:29.06 CBV.dial between SSV and waste2 @18:00:29.47 CTV.dial CSV,avoiding: :air @18:00:29.50
CSV.dial CTV @18:00:29.97 CS.reconfigure :pressurize @18:00:30.02 CS.seek 0ml @18:00:54.01 BubblePoint: Intake @24.1psia, Outlet @0.2psia @18:00:54.02 CBV.dial SSV @18:00:54.38 SS.reconfigure :default @18:00:54.43 SS.seek 10ml @18:00:59.84 delay 15 seconds @18:01:14.97 Evacuated: Intake @23.5psia, Outlet @23.3psia @18:01:14.98 SSV.dial :spit,avoiding: :exhaust @18:01:16.06 SS.reconfigure :exhale @18:01:16.11 SS.seek 0ml @18:01:21.15 SS.reconfigure :fast @18:01:21.19 SS.seek 5ml @18:01:24.10 SSV.dial CBV,avoiding: :exhaust @18:01:24.13
CBV.dial SSV @18:01:25.51 CTV.dial :air,avoiding: :intake @18:01:26.91 CSV.dial CTV @18:01:26.94
SS.seek 10ml @18:01:28.07 CSV.dial :air @18:01:28.53 CS.reconfigure :fast @18:01:28.58 CS.seek 0ml @18:01:29.74
SSV.dial :spit,avoiding: :exhaust @18:01:31.09 SS.reconfigure :exhale @18:01:31.15 SS.seek 0ml @18:01:31.34 CS.stop @18:01:36.11
SS.reconfigure :fast @18:01:36.18 SS.seek 5ml @18:01:39.23 SSV.dial CBV,avoiding: :exhaust @18:01:39.25
CBV.dial SSV @18:01:41.65 SSV.dial :spit,avoiding: :exhaust @18:01:43.01 SS.reconfigure :default @18:01:43.06 SS.seek 0ml @18:01:46.44 SS.stop @18:01:46.48 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @18:01:46.49 Intake.select :close @18:01:46.56 CSV.dial :waste @18:01:46.69 SSV.dial CBV,avoiding: :exhaust @18:01:46.72
CBV.dial SSV @18:01:47.35 CS.seek 0ml @18:01:47.41 CSV.dial :air @18:01:48.10
CTV.dial :air @18:01:48.18 CS.seek 10ml @18:01:48.24
delay 15 seconds @18:02:03.26 SSV.dial :spit,avoiding: :exhaust @18:02:04.62 CTV.dial CSV,avoiding: :intake @18:02:04.65
SS.reconfigure :exhale @18:02:04.75 SS.seek 0ml @18:02:05.18 SSV.dial CBV,avoiding: :exhaust @18:02:12.25 SSV.dial between CBV and plug @18:02:12.74 CBV.dial between SSV and waste2 @18:02:13.12 CSV.dial between air and CTV @18:02:13.43 CS.seek 8.06ml @18:02:18.78 CTV.dial CSV,avoiding: :air @18:02:18.81
CSV.dial CTV @18:02:19.13 CS.reconfigure :pressurize @18:02:19.18 CS.seek 0ml @18:02:38.20 BubblePoint: Intake @24.1psia, Outlet @-0.4psia @18:02:38.21 CBV.dial SSV @18:02:38.55 SS.reconfigure :default @18:02:38.60 SS.seek 10ml @18:02:44.27 delay 15 seconds @18:02:59.40 Evacuated: Intake @22.5psia, Outlet @16.1psia @18:02:59.41 SSV.dial :spit,avoiding: :exhaust @18:03:00.48 SS.reconfigure :exhale @18:03:00.52 SS.seek 0ml @18:03:05.90 SS.reconfigure :fast @18:03:05.95 SS.seek 5ml @18:03:08.55 SSV.dial CBV,avoiding: :exhaust @18:03:08.58
CBV.dial SSV @18:03:09.96 CTV.dial :air,avoiding: :intake @18:03:11.34 CSV.dial CTV @18:03:11.38
SS.seek 10ml @18:03:12.60 CSV.dial :air @18:03:13.03 CS.reconfigure :fast @18:03:13.08 CS.seek 0ml @18:03:14.27
SSV.dial :spit,avoiding: :exhaust @18:03:15.63 SS.reconfigure :exhale @18:03:16.14 SS.seek 0ml @18:03:16.16 CS.stop @18:03:21.43
SS.reconfigure :fast @18:03:21.48 SS.seek 5ml @18:03:24.26 SSV.dial CBV,avoiding: :exhaust @18:03:24.28
CBV.dial SSV @18:03:26.68 SSV.dial :spit,avoiding: :exhaust @18:03:28.02 SS.reconfigure :default @18:03:28.07 SS.seek 0ml @18:03:31.08 SS.stop @18:03:31.12 Lysing: Adding 2.0ml AqMeOH @18:03:31.13 CBV.dial between CSV and waste1,avoiding: :DBV @18:03:32.65 CTV.dial CSV @18:03:34.03 CSV.dial CSR @18:03:34.82 Pulling 1ml of CSR.AqMeOH @18:03:34.83 CSR.select :AqMeOH @18:03:34.94 CS.reconfigure :default @18:03:34.99 CS.seek 1ml @18:03:38.45 delay 5 seconds @18:03:43.47 CSR.select :air @18:03:43.64 CS.seek 6ml @18:03:57.28 delay 2 seconds @18:03:59.29 CSV.dial :waste2 @18:03:59.75 CS.reconfigure :fast @18:03:59.80 CS.seek 0ml @18:04:14.43 CSV.dial CSR @18:04:14.90 Pulling 2ml of CSR.AqMeOH @18:04:14.91 CSR.select :AqMeOH @18:04:15.03 CS.reconfigure :default @18:04:15.08 CS.seek 2ml @18:04:21.00 delay 5 seconds @18:04:26.02 CSR.select :air @18:04:26.19 CS.seek 5ml @18:04:34.68 delay 2 seconds @18:04:36.70 CSV.dial CTV @18:04:37.16 CS.seek 1ml @18:04:48.38 Before lysing Collection Puck Heater at 14.1C/15.5psia @18:04:48.40 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @18:04:48.42 CH.seek 70C,8:00 @18:07:20.14 CH.hold 10:00,70C @18:07:20.34 Begun lysing Collection Puck Heater at 68.1C/22.6psia @18:17:20.36 Ended lysing Collection Puck Heater at 70C/18.6psia @18:17:20.37 CS.seek 0ml @18:17:23.74 CTV.dial between spare and air,avoiding: :intake @18:17:24.80 CSV.dial :air @18:17:25.25 CS.seek 5ml @18:17:38.92 CSV.dial CTV @18:17:39.38 delay 2 seconds @18:17:41.39 CSV.dial :air @18:17:41.85 delay 2 seconds @18:17:43.86 CSV.dial CTV @18:17:44.32 delay 2 seconds @18:17:46.34 CSV.dial :air @18:17:46.78 delay 2 seconds @18:17:48.81 CS.seek 0ml @18:18:02.51 Puck @27.2psia after final lysis pressurization @18:18:02.52 Recovering Lysate into Collection syringe @18:18:02.53 CSV.dial CBV @18:18:03.53 CBV.dial CSV @18:18:03.92 CS.seek 4.999ml @18:18:17.58 delay 5 seconds @18:18:22.60 CTV.dial :air @18:18:23.12 CS.seek 6.999ml @18:18:29.05 delay 5 seconds @18:18:34.06 CSV.dial :air2 @18:18:34.51 CS.seek 10ml @18:18:43.02 CSV.dial between air and CTV,avoiding: CBV @18:18:44.38 Lysing Complete @18:18:44.45 Storing sh1 puck in Collection Clamp to tube 3 @18:18:44.63 SC.reconfigure :tight @18:18:44.68 SC.seek 3 @18:18:48.37 SC.configure :limp @18:18:49.02 Elbow.reconfigure :tight @18:18:49.08 Elbow.seek 3 @18:18:50.84 Forearm.reconfigure :tight @18:18:50.89 Forearm.seek 3 + 800 counts @18:18:51.84 SE.reconfigure :pick @18:18:51.90 SE.seek :beamBreak @18:18:53.80 SE.configure :default @18:18:53.96 Elbow.reconfigure :limp @18:18:54.01 Forearm.reconfigure :loose @18:18:54.06 Forearm.seek 3 @18:18:54.45 Elbow.hold @18:18:54.51 Elbow.reconfigure :tight @18:18:54.61 SE.seek 11.1 Pucks below Top Plate @18:18:55.02 Forearm.force -20 @18:18:55.07 Forearm.seek 3 + 292 counts @18:18:55.34 Forearm.reconfigure :tight @18:18:55.38 Forearm.seek 3 + 800 counts @18:18:55.74 SE.seek 12.1 Pucks below Top Plate @18:18:56.38 Forearm.reconfigure :loose @18:18:56.42 Forearm.seek :retract @18:18:57.37 Elbow.seek CC @18:18:59.02 Elbow.reconfigure :loose @18:18:59.12 Forearm.seek CC @18:19:00.09 Hand.close @18:19:00.80 CC.seek :open @18:19:05.00 Forearm.seek :retract @18:19:06.05 Elbow.reconfigure :tight @18:19:06.09 Elbow.seek 3 @18:19:07.80 Elbow.reconfigure :limp @18:19:07.85 Forearm.seek 3 @18:19:09.43 Elbow.hold @18:19:09.50 Elbow.reconfigure :tight @18:19:09.55 Hand.open @18:19:10.42 Forearm.force -20 @18:19:10.46 Forearm.seek 3 + 319 counts @18:19:10.75 Forearm.reconfigure :tight @18:19:10.79 Forearm.seek 3 + 800 counts @18:19:11.12 SE.seek :down @18:19:12.26
Forearm.reconfigure :loose @18:19:12.31 Forearm.seek :retract @18:19:12.68 SE.stop @18:19:13.23
Tranferring DA @18:19:13.25 Beginning samplePassC @18:19:13.26 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:19:13.45 Elbow.seek FlushPuck::Garage @18:19:15.22 Elbow.reconfigure :loose @18:19:15.33 Forearm.seek :garage @18:19:16.31 Hand.close @18:19:17.16 Forearm.seek :retract @18:19:18.33 Elbow.reconfigure :tight @18:19:18.37 Elbow.seek CC @18:19:21.24 Elbow.reconfigure :loose @18:19:21.34 Forearm.reconfigure :tight @18:19:21.39 Forearm.seek CC @18:19:22.66 CC.seek :closed @18:19:26.67 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.93 @18:19:26.75 Hand.open @18:19:27.60 Forearm.reconfigure :loose @18:19:27.66 Forearm.seek :retract @18:19:28.78 PSV.dial PSR @18:19:30.27 Pulling 1.5ml of PSR.flush @18:19:30.28 PSR.select :flush @18:19:30.40 PS.seek 1.5ml @18:19:46.63 delay 5 seconds @18:19:51.65 PSV.dial :waste1 @18:19:52.13 PS.seek 0ml @18:20:08.25 PSV.dial PSR @18:20:08.72 PSR.select :air @18:20:08.84 PS.seek 2.5ml @18:20:35.19 PRV.dial CBV,avoiding: :ab2 @18:20:35.22
CBV.dial PRV @18:20:36.97 PSV.dial :mixloop @18:20:38.00 CTV.dial :air,avoiding: :intake @18:20:38.08 PTV.dial :PRVmixing @18:20:39.38 PS.seek 0ml @18:21:05.88 PSV.dial :air @18:21:06.46 PS.seek 2.5ml @18:21:32.78 PSV.dial :mixloop @18:21:33.24 PS.seek 0ml @18:21:59.62 PSV.dial :air @18:22:00.10 PS.seek 2.5ml @18:22:26.44 PSV.dial :mixloop @18:22:26.91 PS.seek 0ml @18:22:53.35 Garaging FlushPuck in Collection Clamp @18:22:53.42 Elbow.reconfigure :tight @18:22:53.47 Elbow.seek CC @18:22:53.58 Elbow.reconfigure :loose @18:22:53.68 Forearm.seek CC @18:22:54.70 Hand.close @18:22:55.42 CC.seek :open @18:22:59.95 Forearm.seek :retract @18:23:00.95 Elbow.reconfigure :tight @18:23:01.00 Elbow.seek :garage @18:23:03.87 Elbow.reconfigure :loose @18:23:03.98 Forearm.reconfigure :tight @18:23:04.02 Forearm.seek :garage @18:23:05.15 Hand.open @18:23:05.98 Forearm.reconfigure :loose @18:23:06.02 Forearm.seek :retract @18:23:07.02 PSV.dial :mixloop @18:23:07.19 PTV.dial :PRVmixing @18:23:07.20 PRV.dial CBV @18:23:07.23
CBV.dial PRV @18:23:07.37 CTV.dial :air,avoiding: :intake @18:23:07.45 Loading lyfil puck from tube 4 into Collection Clamp @18:23:07.69 SC.reconfigure :tight @18:23:07.74 SC.seek 4 @18:23:11.36 SC.configure :limp @18:23:11.58 Elbow.reconfigure :tight @18:23:11.63 Elbow.seek 4 @18:23:13.50 Forearm.reconfigure :tight @18:23:13.55 Forearm.seek 4 + 800 counts @18:23:14.63 SE.reconfigure :pick @18:23:14.69 SE.seek :beamBreak @18:23:16.97 SE.configure :default @18:23:17.13 Elbow.seek 4 @18:23:17.32 Forearm.seek 4 + 300 counts @18:23:17.49 Forearm.reconfigure :loose @18:23:17.54 Hand.close @18:23:18.74 SE.seek :down @18:23:18.95
Forearm.seek :retract @18:23:19.92 Elbow.seek CC @18:23:20.77 SE.stop @18:23:21.46
Elbow.reconfigure :loose @18:23:21.56 Forearm.reconfigure :tight @18:23:21.61 Forearm.seek CC @18:23:22.83 CC.seek :closed @18:23:26.81 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.01 @18:23:26.82 Hand.open @18:23:27.61 Forearm.reconfigure :loose @18:23:27.66 Forearm.seek :retract @18:23:28.60 CTV.dial CSV,avoiding: :intake @18:23:29.98 CSV.dial CTV @18:23:30.43 PS.seek 2.5ml @18:23:56.87 CS.seek 5ml @18:24:10.64 delay 8 seconds @18:24:18.66 PSV.dial :air @18:24:19.11 delay 2 seconds @18:24:21.13 PSV.dial :waste2 @18:24:21.88 PS.seek 0ml @18:24:48.19 CBV.dial between PRV and waste1 @18:24:48.51 CSV.dial :air @18:24:48.95 CS.seek 10ml @18:25:02.51 CSV.dial CTV @18:25:02.97 CS.seek 0ml @18:25:29.57 CBV.dial :waste1 @18:25:29.90 delay 5 seconds @18:25:34.93 CTV.dial :air,avoiding: :intake @18:25:36.78 Storing lyfil puck in Collection Clamp to tube 3 @18:25:36.97 SC.reconfigure :tight @18:25:37.02 SC.seek 3 @18:25:40.68 SC.configure :limp @18:25:40.90 Elbow.reconfigure :tight @18:25:40.94 Elbow.seek 3 @18:25:42.67 Forearm.reconfigure :tight @18:25:42.72 Forearm.seek 3 + 800 counts @18:25:43.72 SE.reconfigure :pick @18:25:43.78 SE.seek :beamBreak @18:25:45.75 SE.configure :default @18:25:45.92 Elbow.reconfigure :limp @18:25:45.97 Forearm.reconfigure :loose @18:25:46.01 Forearm.seek 3 @18:25:46.38 Elbow.hold @18:25:46.45 Elbow.reconfigure :tight @18:25:46.55 SE.seek 12.1 Pucks below Top Plate @18:25:47.04 Forearm.force -20 @18:25:47.08 Forearm.seek 3 + 303 counts @18:25:47.34 Forearm.reconfigure :tight @18:25:47.39 Forearm.seek 3 + 800 counts @18:25:47.75 SE.seek 13.1 Pucks below Top Plate @18:25:48.34 Forearm.reconfigure :loose @18:25:48.39 Forearm.seek :retract @18:25:49.32 Elbow.seek CC @18:25:50.96 Elbow.reconfigure :loose @18:25:51.06 Forearm.seek CC @18:25:52.02 Hand.close @18:25:53.17 CC.seek :open @18:25:57.38 Forearm.seek :retract @18:25:58.41 Elbow.reconfigure :tight @18:25:58.46 Elbow.seek 3 @18:26:00.17 Elbow.reconfigure :limp @18:26:00.22 Forearm.seek 3 @18:26:01.36 Elbow.hold @18:26:01.43 Elbow.reconfigure :tight @18:26:01.48 Hand.open @18:26:02.28 Forearm.force -20 @18:26:02.33 Forearm.seek 3 + 319 counts @18:26:02.61 Forearm.reconfigure :tight @18:26:02.66 Forearm.seek 3 + 800 counts @18:26:02.99 SE.seek :down @18:26:04.13
Forearm.reconfigure :loose @18:26:04.18 Forearm.seek :retract @18:26:04.45 SE.stop @18:26:05.13
PSV.dial :air @18:26:05.88 PS.seek 2.5ml @18:26:32.22 delay 1 second @18:26:33.23 PSV.dial :mixloop @18:26:33.69 delay 1 second @18:26:34.70 PSV.dial :air @18:26:35.17 delay 1 second @18:26:36.19 PSV.dial :mixloop @18:26:36.66 delay 1 second @18:26:37.67 PSV.dial :air @18:26:38.14 delay 1 second @18:26:39.16 PS.seek 0ml @18:27:05.53 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @18:27:05.72 Elbow.seek FlushPuck::Garage @18:27:07.93 Elbow.reconfigure :loose @18:27:08.04 Forearm.seek :garage @18:27:09.11 Hand.close @18:27:10.03 Forearm.seek :retract @18:27:11.10 Elbow.reconfigure :tight @18:27:11.15 Elbow.seek PC @18:27:12.47 Elbow.reconfigure :loose @18:27:12.57 Forearm.reconfigure :tight @18:27:12.62 Forearm.seek PC @18:27:14.00 PC.seek :closed @18:27:18.47 Processing Clamp onSpring/offSpring for 12/27 samples, on/off torque 1.53 @18:27:18.54 Hand.open @18:27:19.35 Forearm.reconfigure :loose @18:27:19.39 Forearm.seek :retract @18:27:20.49 PRV.dial :air @18:27:20.93 PSV.dial :mixloop @18:27:21.48 PS.seek 0.3ml @18:27:25.31 PSV.dial PSR @18:27:26.36 Pulling 0.4ml of PSR.air @18:27:26.37 PSR.select :air @18:27:26.39 PS.seek 0.7ml @18:27:31.25 delay 3 seconds @18:27:34.26 PSR.select :air @18:27:34.29 PS.seek 2.5ml @18:27:53.56 delay 3 seconds @18:27:56.58 PSV.dial :mixloop @18:27:57.64 PTV.dial :mixing @18:27:58.39 PBV.dial :waste2 @18:27:59.38 PS.seek 0ml @18:28:25.79 Garaging FlushPuck in Processing Clamp @18:28:25.86 Elbow.reconfigure :tight @18:28:25.91 Elbow.seek PC @18:28:26.09 Elbow.reconfigure :loose @18:28:26.20 Forearm.seek PC @18:28:27.28 Hand.close @18:28:28.04 PC.seek :open @18:28:32.52 Forearm.seek :retract @18:28:33.54 Elbow.reconfigure :tight @18:28:33.58 Elbow.seek :garage @18:28:34.96 Elbow.reconfigure :loose @18:28:35.11 Forearm.reconfigure :tight @18:28:35.16 Forearm.seek :garage @18:28:36.24 Hand.open @18:28:37.10 Forearm.reconfigure :loose @18:28:37.15 Forearm.seek :retract @18:28:38.13 Loading da puck from tube 4 into Processing Clamp @18:28:38.36 SC.reconfigure :tight @18:28:38.41 SC.seek 4 @18:28:42.01 SC.configure :limp @18:28:42.23 Elbow.reconfigure :tight @18:28:42.27 Elbow.seek 4 @18:28:44.05 Forearm.reconfigure :tight @18:28:44.09 Forearm.seek 4 + 800 counts @18:28:45.10 SE.reconfigure :pick @18:28:45.16 SE.seek :beamBreak @18:28:47.48 SE.configure :default @18:28:47.64 Elbow.seek 4 @18:28:47.83 Forearm.seek 4 + 300 counts @18:28:48.34 Forearm.reconfigure :loose @18:28:48.39 Hand.close @18:28:49.17 SE.seek :down @18:28:49.38
Forearm.seek :retract @18:28:50.38 Elbow.seek PC @18:28:51.31 SE.stop @18:28:52.92
Elbow.reconfigure :loose @18:28:53.02 Forearm.reconfigure :tight @18:28:53.07 Forearm.seek PC @18:28:54.27 PC.seek :closed @18:28:58.69 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.52 @18:28:58.70 Hand.open @18:28:59.48 Forearm.reconfigure :loose @18:28:59.52 Forearm.seek :retract @18:29:00.51 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @18:29:00.53 PH.seek 28C,5:00 @18:29:45.32 PH.hold 2:00:00,28C @18:29:45.37 delay 1:30 @18:31:15.41 PSR.select :air @18:31:15.43 PSV.dial PSR @18:31:16.48 Pulling 0.5ml of PSR.wash @18:31:16.49 PSR.select :wash @18:31:16.61 PS.seek 0.5ml @18:31:22.56 delay 5 seconds @18:31:27.58 PSR.select :air @18:31:27.70 PS.seek 2ml @18:31:43.84 PTV.dial :puck @18:31:44.57 PSV.dial PTV @18:31:45.34 PBV.dial :waste1 @18:31:46.77 PS.seek 1.5ml @18:31:52.71 delay 3 seconds @18:31:55.73 PBV.dial between PSV and waste1 @18:31:56.11 PS.seek 0ml @18:32:12.28 delay 5:00 @18:37:12.29 recoverPSRreagent08 @18:37:12.31 PTV.dial :puck @18:37:12.38 PBV.dial :waste1 @18:37:12.77 delay 30 seconds @18:37:42.80 PBV.dial between PSV and waste1 @18:37:43.18 PSR.select :air @18:37:43.19 PSV.dial PSR @18:37:44.07 PS.reconfigure :fast @18:37:44.12 PS.seek 2.5ml @18:38:07.43 PSV.dial PTV @18:38:08.29 delay 5 seconds @18:38:13.31 PSV.dial PSR @18:38:14.04 delay 5 seconds @18:38:19.06 PSV.dial PTV @18:38:19.80 delay 5 seconds @18:38:24.82 PSV.dial :air @18:38:25.67 PS.seek 0ml @18:38:48.74 PTV.dial :PRVmixing,via: PRV @18:38:50.05 PSV.dial :mixloop @18:38:50.52 PRV.dial :ab1 @18:38:51.28 PS.reconfigure :slow @18:38:51.32 PS.seek 0.15ml @18:38:54.93 delay 2 seconds @18:38:56.95 CBV.dial PRV @18:38:56.97
PRV.dial CBV @18:38:57.56 PS.seek 0.34ml @18:39:01.90 delay 2 seconds @18:39:03.91 CBV.dial between PRV and PSV @18:39:04.23 PRV.dial :air @18:39:04.68 PS.seek 0.9ml @18:39:16.61 PRV.dial :waste2 @18:39:17.37 PS.seek 0.5ml @18:39:26.05 addPMLReagent08 @18:39:26.06 PSV.dial :air @18:39:26.52 PS.reconfigure :default @18:39:26.57 PS.seek 2ml @18:39:42.77 PSV.dial :PML @18:39:43.24 PTV.dial :mixing @18:39:43.99 PBV.dial :waste1 @18:39:44.43 PS.seek 1.85ml @18:39:46.77 PBV.dial between PSV and waste1 @18:39:47.15 PS.seek 0ml @18:40:06.86 delay 2 seconds @18:40:08.88 Beginning samplePassC @18:40:08.90 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:40:09.08 Elbow.reconfigure :tight @18:40:09.12 Elbow.seek FlushPuck::Garage @18:40:10.53 Elbow.reconfigure :loose @18:40:10.63 Forearm.seek :garage @18:40:11.69 Hand.close @18:40:12.64 Forearm.seek :retract @18:40:13.70 Elbow.reconfigure :tight @18:40:13.74 Elbow.seek CC @18:40:16.55 Elbow.reconfigure :loose @18:40:17.08 Forearm.reconfigure :tight @18:40:17.14 Forearm.seek CC @18:40:18.39 CC.seek :closed @18:40:22.40 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.06 @18:40:22.48 Hand.open @18:40:23.34 Forearm.reconfigure :loose @18:40:23.39 Forearm.seek :retract @18:40:24.37 PSV.dial PSR @18:40:25.41 Pulling 1.5ml of PSR.flush @18:40:25.42 PSR.select :flush @18:40:25.54 PS.seek 1.5ml @18:40:41.68 delay 5 seconds @18:40:46.69 PSV.dial :waste1 @18:40:47.15 PS.seek 0ml @18:41:03.30 PSV.dial PSR @18:41:03.77 PSR.select :air @18:41:03.88 PS.seek 2.5ml @18:41:30.27 PRV.dial CBV,avoiding: :ab2 @18:41:30.30
CBV.dial PRV @18:41:31.94 PSV.dial :mixloop @18:41:32.97 CTV.dial :air,avoiding: :intake @18:41:33.04 PTV.dial :PRVmixing @18:41:33.79 PS.seek 0ml @18:42:00.14 PSV.dial :air @18:42:00.60 PS.seek 2.5ml @18:42:27.11 PSV.dial :mixloop @18:42:27.59 PS.seek 0ml @18:42:53.92 PSV.dial :air @18:42:54.40 PS.seek 2.5ml @18:43:20.80 PSV.dial :mixloop @18:43:21.27 PS.seek 0ml @18:43:47.58 Garaging FlushPuck in Collection Clamp @18:43:48.11 Elbow.reconfigure :tight @18:43:48.15 Elbow.seek CC @18:43:48.28 Elbow.reconfigure :loose @18:43:48.38 Forearm.seek CC @18:43:49.33 Hand.close @18:43:50.02 CC.seek :open @18:43:54.14 Forearm.seek :retract @18:43:55.16 Elbow.reconfigure :tight @18:43:55.21 Elbow.seek :garage @18:43:58.07 Elbow.reconfigure :loose @18:43:58.18 Forearm.reconfigure :tight @18:43:58.22 Forearm.seek :garage @18:43:59.31 Hand.open @18:44:00.08 Forearm.reconfigure :loose @18:44:00.12 Forearm.seek :retract @18:44:01.10 delayUntil "18:48:08.87" @18:44:01.12 Whole Cell Archive sampling 1000ml @18:44:01.14 Loading wcr puck from tube 4 into Collection Clamp @18:44:01.38 SC.reconfigure :tight @18:44:01.43 SC.seek 4 @18:44:01.50 SC.configure :limp @18:44:01.72 Elbow.reconfigure :tight @18:44:01.77 Elbow.seek 4 @18:44:03.72 Forearm.reconfigure :tight @18:44:03.77 Forearm.seek 4 + 800 counts @18:44:04.78 SE.reconfigure :pick @18:44:04.85 SE.seek :beamBreak @18:44:07.22 SE.configure :default @18:44:07.80 Elbow.seek 4 @18:44:08.08 Forearm.seek 4 + 300 counts @18:44:08.36 Forearm.reconfigure :loose @18:44:08.41 Hand.close @18:44:09.16 SE.seek :down @18:44:09.37 Forearm.seek :retract @18:44:10.32 Elbow.seek CC @18:44:11.37 SE.stop @18:44:11.95 Elbow.reconfigure :loose @18:44:12.06 Forearm.reconfigure :tight @18:44:12.11 Forearm.seek CC @18:44:13.42 CC.seek :closed @18:44:17.42 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.05 @18:44:17.43 Hand.open @18:44:18.20 Forearm.reconfigure :loose @18:44:18.25 Forearm.seek :retract @18:44:19.30 collectSample 1000ml w/bubble point of 35psi @18:44:19.32 Priming 7ml @18:44:19.33 Intake.select :close @18:44:19.34 Exhaust.select :close @18:44:19.35 SS.hold @18:44:19.56 Calibrating pressure sensors @18:44:19.58 CTV.dial :air @18:44:19.65 SSV.dial :spit,avoiding: :exhaust @18:44:19.74 SS.reconfigure :fast @18:44:19.79 SS.seek 5ml @18:44:22.66 SSV.dial CBV @18:44:22.69 CBV.dial SSV @18:44:24.55 delay 3.8 seconds @18:44:28.48 Sensor limits: Intake max 96.9psia; Outlet max 107.9psia @18:44:28.50 SSV.dial :spit @18:44:29.87 SS.seek 0ml @18:44:32.50 SSV.dial CBV @18:44:33.85 CTV.dial :intake @18:44:34.62 SSV.dial CBV @18:44:34.66 CBV.dial SSV @18:44:34.81 Allowing 40s for 7.0ml of remaining 7.0ml @18:44:34.82 Intake.select :open @18:44:36.99 Intake @14.6psia, vacuum from 9.1 to 12.1psi @18:44:37.01 SS.reconfigure :inhaling 2.17ml/s @18:44:37.05 SS.seek 7ml @18:44:43.87 Stroke completed -- waiting for pressure to equalize ... @18:44:43.89 SS.reconfigure :exhale @18:44:43.93 SS.hold @18:44:51.46 Intake.select :close @18:44:51.62 SSV.dial :exhaust @18:44:52.38 Exhaust.select :open @18:44:52.49 SS.seek 0ml @18:44:57.17 Exhaust.select :close @18:44:57.29 Sampling 1000.0ml @18:44:57.30 Intake.select :close @18:44:57.31 Exhaust.select :close @18:44:57.32 SS.reconfigure :default @18:44:57.37 SS.hold @18:44:57.58 CTV.dial :intake @18:44:57.60 SSV.dial CBV @18:44:57.64 CBV.dial SSV @18:44:58.41 Allowing 130s for 25.0ml of remaining 1000.0ml @18:44:58.42 Intake.select :open @18:45:00.59 Intake @14.1psia, vacuum from 8.6 to 11.6psi @18:45:00.61 SS.reconfigure :inhaling 2.17ml/s @18:45:00.65 SS.seek 25ml @18:45:06.82 Vacuum too high -- waiting up to 40 seconds @18:45:06.84 SS.reconfigure :exhale @18:45:06.89 SS.hold @18:45:09.34 Intake @12.0psia, vacuum from 6.5 to 9.5psi @18:45:09.36 SS.reconfigure :inhaling 1.95ml/s @18:45:09.41 SS.seek 25ml @18:45:12.04 Vacuum too high -- waiting up to 40 seconds @18:45:12.06 SS.reconfigure :exhale @18:45:12.11 SS.hold @18:45:18.66 Intake @11.9psia, vacuum from 6.4 to 9.4psi @18:45:18.68 SS.reconfigure :inhaling 1.77ml/s @18:45:18.74 SS.seek 25ml @18:45:22.20 Vacuum too high -- waiting up to 40 seconds @18:45:22.22 SS.reconfigure :exhale @18:45:22.27 SS.hold @18:45:24.15 Intake @9.7psia, vacuum from 4.2 to 7.2psi @18:45:24.17 SS.reconfigure :inhaling 1.59ml/s @18:45:24.22 SS.seek 25ml @18:45:26.98 Vacuum too high -- waiting up to 40 seconds @18:45:26.99 SS.reconfigure :exhale @18:45:27.05 SS.hold @18:45:34.81 Intake @13.9psia, vacuum from 8.4 to 11.4psi @18:45:34.83 SS.reconfigure :inhaling 1.43ml/s @18:45:34.88 SS.seek 25ml @18:45:39.56 Stroke completed -- waiting for pressure to equalize ... @18:45:39.57 SS.reconfigure :exhale @18:45:39.62 SS.hold @18:45:45.27 Intake.select :close @18:45:45.43 SSV.dial :exhaust @18:45:46.19 Exhaust.select :open @18:45:46.30 SS.seek 0ml @18:45:55.67 Exhaust.select :close @18:45:55.78 SSV.dial CBV @18:45:55.80 CBV.dial SSV @18:45:56.61 Allowing 130s for 25.0ml of remaining 975.0ml @18:45:56.62 Intake.select :open @18:45:58.79 Intake @13.6psia, vacuum from 8.1 to 11.1psi @18:45:58.81 SS.reconfigure :inhaling 1.43ml/s @18:45:58.85 SS.seek 25ml @18:46:03.94 Vacuum too high -- waiting up to 40 seconds @18:46:03.96 SS.reconfigure :exhale @18:46:04.01 SS.hold @18:46:06.47 Intake @12.0psia, vacuum from 6.5 to 9.5psi @18:46:06.49 SS.reconfigure :inhaling 1.29ml/s @18:46:06.54 SS.seek 25ml @18:46:09.82 Vacuum too high -- waiting up to 40 seconds @18:46:09.84 SS.reconfigure :exhale @18:46:09.89 SS.hold @18:46:13.57 Intake @13.0psia, vacuum from 7.5 to 10.5psi @18:46:13.59 SS.reconfigure :inhaling 1.16ml/s @18:46:13.64 SS.seek 25ml @18:46:17.79 Vacuum too high -- waiting up to 40 seconds @18:46:17.80 SS.reconfigure :exhale @18:46:17.86 SS.hold @18:46:20.43 Intake @12.3psia, vacuum from 6.8 to 9.8psi @18:46:20.45 SS.reconfigure :inhaling 1.04ml/s @18:46:20.50 SS.seek 25ml @18:46:25.16 Vacuum too high -- waiting up to 40 seconds @18:46:25.18 SS.reconfigure :exhale @18:46:25.23 SS.hold @18:46:28.89 Intake @12.9psia, vacuum from 7.4 to 10.4psi @18:46:28.91 SS.reconfigure :inhaling 0.94ml/s @18:46:28.95 SS.seek 25ml @18:46:35.29 Vacuum too high -- waiting up to 40 seconds @18:46:35.30 SS.reconfigure :exhale @18:46:35.36 SS.hold @18:46:39.00 Intake @13.7psia, vacuum from 8.2 to 11.2psi @18:46:39.02 SS.reconfigure :inhaling 0.85ml/s @18:46:39.07 SS.seek 25ml @18:46:42.76 Stroke completed -- waiting for pressure to equalize ... @18:46:42.77 SS.reconfigure :exhale @18:46:42.82 SS.hold @18:46:46.82 Intake.select :close @18:46:46.98 SSV.dial :exhaust @18:46:47.75 Exhaust.select :open @18:46:47.88 SS.seek 0ml @18:46:57.15 Exhaust.select :close @18:46:57.27 SSV.dial CBV @18:46:57.30 CBV.dial SSV @18:46:58.09 Allowing 130s for 25.0ml of remaining 950.0ml @18:46:58.11 Intake.select :open @18:47:00.27 Intake @13.8psia, vacuum from 8.3 to 11.3psi @18:47:00.29 SS.reconfigure :inhaling 0.85ml/s @18:47:00.34 SS.seek 25ml @18:47:11.38 Vacuum too high -- waiting up to 40 seconds @18:47:11.40 SS.reconfigure :exhale @18:47:11.45 SS.hold @18:47:13.57 Intake @11.4psia, vacuum from 5.9 to 8.9psi @18:47:13.59 SS.reconfigure :inhaling 0.77ml/s @18:47:13.64 SS.seek 25ml @18:47:17.41 Vacuum too high -- waiting up to 40 seconds @18:47:17.43 SS.reconfigure :exhale @18:47:17.48 SS.hold @18:47:22.73 Intake @11.9psia, vacuum from 6.4 to 9.4psi @18:47:22.75 SS.reconfigure :inhaling 0.70ml/s @18:47:22.79 SS.seek 25ml @18:47:31.85 Vacuum too high -- waiting up to 40 seconds @18:47:31.87 SS.reconfigure :exhale @18:47:31.92 SS.hold @18:47:35.57 Intake @11.8psia, vacuum from 6.3 to 9.3psi @18:47:35.59 SS.reconfigure :inhaling 0.62ml/s @18:47:35.64 SS.seek 25ml @18:47:46.44 Vacuum too high -- waiting up to 40 seconds @18:47:46.46 SS.reconfigure :exhale @18:47:46.51 SS.hold @18:47:49.31 Intake @10.4psia, vacuum from 4.9 to 7.9psi @18:47:49.33 SS.reconfigure :inhaling 0.56ml/s @18:47:49.38 SS.seek 25ml @18:47:52.14 Stroke completed -- waiting for pressure to equalize ... @18:47:52.15 SS.reconfigure :exhale @18:47:52.20 SS.hold @18:47:56.35 Intake.select :close @18:47:56.51 SSV.dial :exhaust @18:47:57.28 Exhaust.select :open @18:47:57.40 SS.seek 0ml @18:48:06.73 Exhaust.select :close @18:48:06.84 SSV.dial CBV @18:48:06.87 CBV.dial SSV @18:48:07.68 Allowing 130s for 25.0ml of remaining 925.0ml @18:48:07.70 Intake.select :open @18:48:08.88 recoverPRVreagent08 @18:48:08.89 PTV.dial :mixing @18:48:09.62 PBV.dial :waste1 @18:48:09.86 Intake @12.0psia, vacuum from 6.5 to 9.5psi @18:48:09.89 SS.reconfigure :inhaling 0.56ml/s @18:48:09.94 SS.seek 25ml @18:48:10.01 delay 30 seconds @18:48:22.78 Vacuum too high -- waiting up to 40 seconds @18:48:22.80 SS.reconfigure :exhale @18:48:22.85 SS.hold @18:48:28.13 Intake @12.8psia, vacuum from 7.3 to 10.3psi @18:48:28.15 SS.reconfigure :inhaling 0.51ml/s @18:48:28.20 SS.seek 25ml @18:48:36.88 Vacuum too high -- waiting up to 40 seconds @18:48:36.89 SS.reconfigure :exhale @18:48:36.95 SS.hold @18:48:40.03 PBV.dial between PSV and waste1 @18:48:40.42 PSV.dial :air @18:48:40.90 PS.reconfigure :fast @18:48:40.94 PS.seek 2.5ml @18:48:42.44 Intake @12.7psia, vacuum from 7.2 to 10.2psi @18:48:42.46 SS.reconfigure :inhaling 0.46ml/s @18:48:42.51 SS.seek 25ml @18:48:53.69 Vacuum too high -- waiting up to 40 seconds @18:48:53.71 SS.reconfigure :exhale @18:48:53.76 SS.hold @18:48:59.38 Intake @12.8psia, vacuum from 7.3 to 10.3psi @18:48:59.40 SS.reconfigure :inhaling 0.42ml/s @18:48:59.45 SS.seek 25ml @18:49:04.46 PSV.dial :mixloop @18:49:04.92 delay 2 seconds @18:49:06.94 PSV.dial :air @18:49:07.40 delay 2 seconds @18:49:09.42 PSV.dial :mixloop @18:49:09.89 delay 2 seconds @18:49:11.91 PSV.dial :air @18:49:12.38 PS.seek 0ml @18:49:14.41 Vacuum too high -- waiting up to 40 seconds @18:49:14.43 SS.reconfigure :exhale @18:49:14.49 SS.hold @18:49:20.38 Intake @13.0psia, vacuum from 7.5 to 10.5psi @18:49:20.40 SS.reconfigure :inhaling 0.38ml/s @18:49:20.44 SS.seek 25ml @18:49:28.46 Stroke completed -- waiting for pressure to equalize ... @18:49:28.47 SS.reconfigure :exhale @18:49:28.52 SS.hold @18:49:35.51 washCycleDA #1 of 2 @18:49:35.52 PSR.select :air @18:49:35.54 PSV.dial PSR @18:49:36.87 Pulling 0.5ml of PSR.wash @18:49:36.88 PSR.select :wash @18:49:37.00 PS.reconfigure :default @18:49:37.09 PS.seek 0.5ml @18:49:37.25 Intake.select :close @18:49:37.80 SSV.dial :exhaust @18:49:38.64 Exhaust.select :open @18:49:38.76 SS.seek 0ml @18:49:43.04 delay 5 seconds @18:49:47.68 Exhaust.select :close @18:49:47.79 SSV.dial CBV @18:49:47.83 CBV.dial SSV @18:49:48.06 PSR.select :air @18:49:48.19 PS.seek 2ml @18:49:48.59 Allowing 130s for 25.0ml of remaining 900.0ml @18:49:48.61 Intake.select :open @18:49:50.77 Intake @14.1psia, vacuum from 8.6 to 11.6psi @18:49:50.79 SS.reconfigure :inhaling 0.38ml/s @18:49:50.84 SS.seek 25ml @18:50:04.39 PTV.dial :puck @18:50:05.14 PSV.dial PTV @18:50:05.87 PBV.dial :waste1 @18:50:06.31 PS.seek 1.5ml @18:50:12.23 delay 3 seconds @18:50:12.50 Vacuum too high -- waiting up to 40 seconds @18:50:12.52 SS.reconfigure :exhale @18:50:12.57 SS.hold @18:50:15.25 PBV.dial between PSV and waste1 @18:50:15.63 PS.seek 0ml @18:50:27.05 Intake @13.4psia, vacuum from 7.9 to 10.9psi @18:50:27.07 SS.reconfigure :inhaling 0.33ml/s @18:50:27.12 SS.seek 25ml @18:50:31.79 delay 2:00 @18:50:41.72 Vacuum too high -- waiting up to 40 seconds @18:50:41.74 SS.reconfigure :exhale @18:50:41.79 SS.hold @18:51:04.41 Intake @13.8psia, vacuum from 8.3 to 11.3psi @18:51:04.43 SS.reconfigure :inhaling 0.30ml/s @18:51:04.48 SS.seek 25ml @18:51:27.34 Vacuum too high -- waiting up to 40 seconds @18:51:27.36 SS.reconfigure :exhale @18:51:27.41 SS.hold @18:51:54.16 Intake @14.1psia, vacuum from 8.6 to 11.6psi @18:51:54.17 SS.reconfigure :inhaling 0.28ml/s @18:51:54.22 SS.seek 25ml @18:51:58.62 SS.reconfigure :default @18:51:58.67 SS.hold @18:51:58.83 Intake.select :close @18:51:59.00 ShallowSampler::Clogged in WCR -- Filtering too Slowly after 120.2ml @18:51:59.01 SSV.dial :exhaust @18:51:59.77 Exhaust.select :open @18:51:59.89 SS.reconfigure :exhale @18:51:59.94 SS.seek 0ml @18:52:08.13 Exhaust.select :close @18:52:08.24 Sampled 120.2ml @18:52:08.26 Drying puck before fixing for 4:00 @18:52:08.27 CBV.dial :waste2b @18:52:09.04 delay 1:00 @18:52:31.82 recoverPSRreagent08 @18:52:31.83 PTV.dial :puck @18:52:31.90 PBV.dial :waste1 @18:52:32.29 delay 30 seconds @18:53:02.31 PBV.dial between PSV and waste1 @18:53:02.70 PSR.select :air @18:53:02.72 PSV.dial PSR @18:53:03.47 PS.reconfigure :fast @18:53:03.52 PS.seek 2.5ml @18:53:09.06 CSV.dial :air @18:53:09.54 CS.reconfigure :fast @18:53:09.59 CS.seek 10ml @18:53:26.79 PSV.dial PTV @18:53:27.54 delay 5 seconds @18:53:32.56 PSV.dial PSR @18:53:33.31 delay 5 seconds @18:53:33.87 Pumping wcr puck from tube 4 up to 35psi... @18:53:33.89 CBV.dial SSV @18:53:35.17 CBV.dial between CSV and waste2b @18:53:36.15 CSV.dial between air and CTV @18:53:36.48 CS.seek 7.114ml @18:53:38.32 PSV.dial PTV @18:53:39.07 delay 5 seconds @18:53:44.02 CTV.dial CSV,avoiding: :air @18:53:44.51 CSV.dial CTV @18:53:44.62 PSV.dial :air @18:53:45.32 CS.reconfigure :pressurize @18:53:46.46 CS.seek 0ml @18:53:46.50 PS.seek 0ml @18:54:04.54 CBV.dial :waste2b @18:54:04.87 CS.reconfigure :default @18:54:04.92 CS.seek 0ml @18:54:06.30 delay 1:00 @18:54:09.62 washCycleDA #2 of 2 @18:54:09.63 PSR.select :air @18:54:09.64 PSV.dial PSR @18:54:10.96 Pulling 0.5ml of PSR.wash @18:54:10.97 PSR.select :wash @18:54:11.09 PS.reconfigure :default @18:54:11.14 PS.seek 0.5ml @18:54:17.01 delay 5 seconds @18:54:22.03 PSR.select :air @18:54:22.15 PS.seek 2ml @18:54:38.26 PTV.dial :puck @18:54:38.27 PSV.dial PTV @18:54:39.02 PBV.dial :waste1 @18:54:39.48 PS.seek 1.5ml @18:54:45.35 delay 3 seconds @18:54:48.37 PBV.dial between PSV and waste1 @18:54:48.77 PS.seek 0ml @18:55:04.88 delay 2:00 @18:55:06.32 CTV.dial :air,avoiding: :intake @18:55:07.71 Fix #1 of 1 (with RNAlater) for 4:00 @18:55:07.72 CSV.dial CSR @18:55:08.16 Pulling 2ml of CSR.RNAlater @18:55:08.17 CSR.select :RNAlater @18:55:08.29 CS.seek 2ml @18:55:14.20 delay 5 seconds @18:55:19.23 CSR.select :air @18:55:19.35 CS.reconfigure :fast @18:55:19.40 CS.seek 10ml @18:55:38.80 Pumping wcr puck from tube 4 up to 35psi... @18:55:38.82 CBV.dial SSV @18:55:40.07 CBV.dial between CSV and waste2b @18:55:41.05 CSV.dial between air and CTV @18:55:41.65 CS.seek 6.986ml @18:55:49.46 CTV.dial CSV,avoiding: :air @18:55:49.49 CSV.dial CTV @18:55:50.90 CS.reconfigure :pressurize @18:55:50.95 CS.seek 0ml @18:56:07.10 delay 4:00 @18:57:04.91 recoverPSRreagent08 @18:57:04.92 PTV.dial :puck @18:57:04.93 PBV.dial :waste1 @18:57:05.32 delay 30 seconds @18:57:35.35 PBV.dial between PSV and waste1 @18:57:35.73 PSR.select :air @18:57:35.75 PSV.dial PSR @18:57:36.50 PS.reconfigure :fast @18:57:36.55 PS.seek 2.5ml @18:57:59.79 PSV.dial PTV @18:58:00.54 delay 5 seconds @18:58:05.56 PSV.dial PSR @18:58:06.30 delay 5 seconds @18:58:11.32 PSV.dial PTV @18:58:12.08 delay 5 seconds @18:58:17.11 PSV.dial :air @18:58:17.87 PS.seek 0ml @18:58:40.91 getPMLReagent08 @18:58:40.92 PRV.dial :air @18:58:41.37 delay 5 seconds @18:58:46.39 PTV.dial :PRVmixing @18:58:47.69 delay 5 seconds @18:58:52.71 PRV.dial :ab2 @18:58:53.16 PTV.dial :PRVmixing @18:58:53.24 PSV.dial :mixloop @18:58:53.71 PS.reconfigure :default @18:58:53.76 PS.seek 0.3ml @18:58:57.60 delay 2 seconds @18:58:59.62 PRV.dial :air @18:59:00.14 PS.seek 0.55ml @18:59:03.52 addPMLReagent08 @18:59:03.53 PSV.dial :air @18:59:04.00 PS.seek 2ml @18:59:19.60 PSV.dial :PML @18:59:20.07 PTV.dial :mixing @18:59:20.82 PBV.dial :waste1 @18:59:21.25 PS.seek 1.85ml @18:59:23.60 PBV.dial between PSV and waste1 @18:59:23.98 PS.seek 0ml @18:59:43.72 delay 2 seconds @18:59:45.74 delay 8:00 @19:00:07.12 CBV.dial :waste2b @19:00:07.47 CS.reconfigure :default @19:00:07.52 CS.seek 0ml @19:00:10.86 delay 1:00 @19:01:10.89 CTV.dial :air,avoiding: :intake @19:07:45.76 recoverPRVreagent08 @19:07:45.77 PTV.dial :mixing @19:07:45.97 PBV.dial :waste1 @19:07:46.36 delay 30 seconds @19:08:16.38 PBV.dial between PSV and waste1 @19:08:16.82 PSV.dial :air @19:08:17.30 PS.reconfigure :fast @19:08:17.35 PS.seek 2.5ml @19:08:40.90 PSV.dial :mixloop @19:08:41.37 delay 2 seconds @19:08:43.39 PSV.dial :air @19:08:44.32 delay 2 seconds @19:08:46.34 PSV.dial :mixloop @19:08:46.81 delay 2 seconds @19:08:48.83 PSV.dial :air @19:08:49.30 PS.seek 0ml @19:09:12.40 washCycleDA #1 of 3 @19:09:12.41 PSR.select :air @19:09:12.43 PSV.dial PSR @19:09:13.89 Pulling 0.5ml of PSR.wash @19:09:13.90 PSR.select :wash @19:09:14.02 PS.reconfigure :default @19:09:14.07 PS.seek 0.5ml @19:09:19.94 delay 5 seconds @19:09:24.96 PSR.select :air @19:09:25.08 PS.seek 2ml @19:09:41.28 PTV.dial :puck @19:09:42.04 PSV.dial PTV @19:09:42.81 PBV.dial :waste1 @19:09:43.25 PS.seek 1.5ml @19:09:49.12 delay 3 seconds @19:09:52.14 PBV.dial between PSV and waste1 @19:09:52.52 PS.seek 0ml @19:10:08.75 delay 2:00 @19:12:08.78 recoverPSRreagent08 @19:12:08.79 PTV.dial :puck @19:12:08.85 PBV.dial :waste1 @19:12:09.25 delay 30 seconds @19:12:39.27 PBV.dial between PSV and waste1 @19:12:39.67 PSR.select :air @19:12:39.69 PSV.dial PSR @19:12:40.44 PS.reconfigure :fast @19:12:40.49 PS.seek 2.5ml @19:13:04.18 PSV.dial PTV @19:13:04.93 delay 5 seconds @19:13:09.95 PSV.dial PSR @19:13:10.70 delay 5 seconds @19:13:15.72 PSV.dial PTV @19:13:16.48 delay 5 seconds @19:13:21.50 PSV.dial :air @19:13:22.27 PS.seek 0ml @19:13:45.59 washCycleDA #2 of 3 @19:13:45.60 PSR.select :air @19:13:45.62 PSV.dial PSR @19:13:46.95 Pulling 0.5ml of PSR.wash @19:13:46.96 PSR.select :wash @19:13:47.08 PS.reconfigure :default @19:13:47.12 PS.seek 0.5ml @19:13:53.09 delay 5 seconds @19:13:58.11 PSR.select :air @19:13:58.23 PS.seek 2ml @19:14:14.40 PTV.dial :puck @19:14:14.41 PSV.dial PTV @19:14:15.15 PBV.dial :waste1 @19:14:15.60 PS.seek 1.5ml @19:14:21.59 delay 3 seconds @19:14:24.61 PBV.dial between PSV and waste1 @19:14:25.00 PS.seek 0ml @19:14:41.18 delay 2:00 @19:16:41.21 recoverPSRreagent08 @19:16:41.22 PTV.dial :puck @19:16:41.23 PBV.dial :waste1 @19:16:41.62 delay 30 seconds @19:17:11.64 PBV.dial between PSV and waste1 @19:17:12.02 PSR.select :air @19:17:12.46 PSV.dial PSR @19:17:13.28 PS.reconfigure :fast @19:17:13.33 PS.seek 2.5ml @19:17:36.71 PSV.dial PTV @19:17:37.46 delay 5 seconds @19:17:42.48 PSV.dial PSR @19:17:43.23 delay 5 seconds @19:17:48.25 PSV.dial PTV @19:17:48.99 delay 5 seconds @19:17:54.01 PSV.dial :air @19:17:54.78 PS.seek 0ml @19:18:17.87 washCycleDA #3 of 3 @19:18:17.88 PSR.select :air @19:18:17.90 PSV.dial PSR @19:18:19.23 Pulling 0.5ml of PSR.wash @19:18:19.24 PSR.select :wash @19:18:19.35 PS.reconfigure :default @19:18:19.40 PS.seek 0.5ml @19:18:25.30 delay 5 seconds @19:18:30.32 PSR.select :air @19:18:30.45 PS.seek 2ml @19:18:46.61 PTV.dial :puck @19:18:46.63 PSV.dial PTV @19:18:47.49 PBV.dial :waste1 @19:18:47.93 PS.seek 1.5ml @19:18:53.90 delay 3 seconds @19:18:56.92 PBV.dial between PSV and waste1 @19:18:57.29 PS.seek 0ml @19:19:13.49 delay 2:00 @19:21:13.52 recoverPSRreagent08 @19:21:13.53 PTV.dial :puck @19:21:13.54 PBV.dial :waste1 @19:21:13.93 delay 30 seconds @19:21:43.95 PBV.dial between PSV and waste1 @19:21:44.35 PSR.select :air @19:21:44.37 PSV.dial PSR @19:21:45.23 PS.reconfigure :fast @19:21:45.28 PS.seek 2.5ml @19:22:08.71 PSV.dial PTV @19:22:09.44 delay 5 seconds @19:22:14.46 PSV.dial PSR @19:22:15.21 delay 5 seconds @19:22:20.23 PSV.dial PTV @19:22:20.97 delay 5 seconds @19:22:25.99 PSV.dial :air @19:22:26.76 PS.seek 0ml @19:22:50.02 Gate.power :camera,:ON @19:22:50.03 Mixing DA Substrates @19:22:50.04 PSR.select :air @19:22:50.06 PSV.dial PSR @19:22:51.42 Pulling 0.25ml of PSR.sub1 @19:22:51.46 PSR.select :sub1 @19:22:51.65 PS.reconfigure :default @19:22:51.76 PS.seek 0.25ml @19:22:55.25 delay 5 seconds @19:23:00.27 Pulling 0.25ml of PSR.sub2 @19:23:00.29 PSR.select :sub2 @19:23:00.40 PS.seek 0.5ml @19:23:03.83 delay 5 seconds @19:23:08.85 PSR.select :air @19:23:08.97 PS.seek 2ml @19:23:25.11 PTV.dial :puck @19:23:25.12 PSV.dial PTV @19:23:25.86 PBV.dial :waste1 @19:23:26.31 PS.seek 1.5ml @19:23:32.30 delay 3 seconds @19:23:35.32 PBV.dial between PSV and waste1 @19:23:36.20 PS.seek 0ml @19:23:52.30 delay 10 seconds @19:24:02.33 recoverPSRreagent08 @19:24:02.34 PTV.dial :puck @19:24:02.35 PBV.dial :waste1 @19:24:02.72 delay 30 seconds @19:24:32.75 PBV.dial between PSV and waste1 @19:24:33.13 PSR.select :air @19:24:33.15 PSV.dial PSR @19:24:33.90 PS.reconfigure :fast @19:24:33.95 PS.seek 2.5ml @19:24:57.67 PSV.dial PTV @19:24:58.44 delay 5 seconds @19:25:03.46 PSV.dial PSR @19:25:04.21 delay 5 seconds @19:25:09.23 PSV.dial PTV @19:25:09.99 delay 5 seconds @19:25:15.01 PSV.dial :air @19:25:15.79 PS.seek 0ml @19:25:39.18 Additional air clear @19:25:39.19 PSR.select :air @19:25:39.20 PSV.dial PSR @19:25:40.55 Pulling 2ml of PSR.air @19:25:40.56 PSR.select :air @19:25:40.58 PS.reconfigure :default @19:25:40.63 PS.seek 2ml @19:26:01.95 delay 5 seconds @19:26:06.98 PTV.dial :puck @19:26:06.99 PSV.dial PTV @19:26:07.74 PBV.dial :waste1 @19:26:08.19 PS.seek 1.5ml @19:26:14.11 delay 3 seconds @19:26:17.13 PBV.dial between PSV and waste1 @19:26:17.52 delay 1 second @19:26:18.54 PSV.dial :waste1 @19:26:19.59 PS.seek 0ml @19:26:35.79 Processing Puck Heater at 28C before imaging @19:26:35.80 PH.coast @19:26:35.90 Imaging da puck in Processing Clamp while storing it to tube 3 @19:26:36.08 SC.reconfigure :tight @19:26:36.13 SC.seek 3 @19:26:39.77 SC.configure :limp @19:26:39.98 Elbow.reconfigure :tight @19:26:40.03 Elbow.seek 3 @19:26:41.72 Forearm.reconfigure :tight @19:26:41.77 Forearm.seek 3 + 800 counts @19:26:42.79 SE.reconfigure :pick @19:26:42.85 SE.seek :beamBreak @19:26:44.66 SE.configure :default @19:26:44.82 Elbow.reconfigure :limp @19:26:44.88 Forearm.reconfigure :loose @19:26:45.43 Forearm.seek 3 @19:26:45.80 Elbow.hold @19:26:45.87 Elbow.reconfigure :tight @19:26:45.97 SE.seek 13.11 Pucks below Top Plate @19:26:46.55 Forearm.force -20 @19:26:46.59 Forearm.seek 3 + 313 counts @19:26:46.89 Forearm.reconfigure :tight @19:26:46.94 Forearm.seek 3 + 800 counts @19:26:47.25 SE.seek 14.11 Pucks below Top Plate @19:26:47.95 Forearm.reconfigure :loose @19:26:48.00 Forearm.seek :retract @19:26:48.96 Elbow.seek PC @19:26:51.50 Elbow.reconfigure :loose @19:26:51.60 Forearm.seek PC @19:26:52.71 Hand.close @19:26:53.48 PC.seek :open @19:26:58.03 Forearm.seek :retract @19:26:59.04 Elbow.reconfigure :tight @19:26:59.09 Elbow.seek 3 @19:27:01.72 Forearm.reconfigure :tight @19:27:01.77 Forearm.seek 3 + 800 counts @19:27:02.80 Hand.open @19:27:03.75 SE.seek 13.87 Pucks below Top Plate @19:27:04.05 SE.reconfigure :seal @19:27:04.10 SE.seek 13.21 Pucks below Top Plate @19:27:04.65 Lights, camera, ACTION! @19:27:04.68 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da25dec0319h236ml.tif @19:27:24.16 Waiting >20 seconds for da to relinquish FlushPuck @19:27:30.29 SX HX9/16 (USB:14): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 22.276 seconds /var/log/esp/da25dec0319h236ml.tif: TIFF Upload Complete @19:27:30.31 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da25dec0319h236ml15s.tif @19:27:45.93 SX HX9/16 (USB:14): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da25dec0319h236ml15s.tif: TIFF Upload Complete @19:27:45.95 PuckCamera: snap -bin=2,2 21.0 /var/log/esp/midres/da25dec0319h236ml21s.tif @19:28:07.57 SX HX9/16 (USB:14): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21 seconds /var/log/esp/midres/da25dec0319h236ml21s.tif: TIFF Upload Complete @19:28:07.58 Gate.power :camera,:OFF @19:28:07.60 DAfans.email "22.276 sec auto-exposure",Subject: "da25dec0319h236ml images!" @19:28:08.02 Elbow.seek 3 @19:28:08.75 Forearm.seek 3 + 800 counts @19:28:08.91 SE.reconfigure :gently @19:28:08.97 SE.seek 13.93 Pucks below Top Plate @19:28:09.73 Elbow.reconfigure :limp @19:28:09.80 Forearm.reconfigure :loose @19:28:09.85 Forearm.seek 3 @19:28:10.24 Elbow.hold @19:28:10.32 Elbow.reconfigure :tight @19:28:10.38 SE.reconfigure :default @19:28:10.43 SE.seek 14.11 Pucks below Top Plate @19:28:10.80 Forearm.force -20 @19:28:10.84 Forearm.seek 3 + 301 counts @19:28:11.14 Forearm.reconfigure :tight @19:28:11.31 Forearm.seek 3 + 800 counts @19:28:11.73 SE.seek :down @19:28:13.01 Forearm.reconfigure :loose @19:28:13.11 Forearm.seek :retract @19:28:13.26 SE.stop @19:28:14.40 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:28:15.00 Elbow.seek FlushPuck::Garage @19:28:16.92 Elbow.reconfigure :loose @19:28:17.13 Forearm.seek :garage @19:28:18.20 Hand.close @19:28:19.32 Forearm.seek :retract @19:28:20.41 Elbow.reconfigure :tight @19:28:20.52 Elbow.seek PC @19:28:22.02 Elbow.reconfigure :loose @19:28:22.22 Forearm.reconfigure :tight @19:28:23.40 Forearm.seek PC @19:28:25.14 PC.seek :closed @19:28:29.70 Processing Clamp onSpring/offSpring for 12/27 samples, on/off torque 1.55 @19:28:29.84 Hand.open @19:28:30.74 Forearm.reconfigure :loose @19:28:30.84 Forearm.seek :retract @19:28:32.04 PSR.select :air @19:28:32.08 Storing wcr puck in Collection Clamp to tube 3 @19:28:32.13 PSV.dial PSR @19:28:32.62 SC.reconfigure :tight @19:28:32.76 Pulling 0.5ml of PSR.flush @19:28:32.80 SC.seek 3 @19:28:32.83 PSR.select :flush @19:28:33.11 SC.configure :limp @19:28:33.34 PS.seek 0.5ml @19:28:33.76 Elbow.reconfigure :tight @19:28:33.86 Elbow.seek 3 @19:28:36.74 Forearm.reconfigure :tight @19:28:36.85 Forearm.seek 3 + 800 counts @19:28:38.47 SE.reconfigure :pick @19:28:38.58 SE.seek :beamBreak @19:28:39.29 delay 5 seconds @19:28:40.31 SE.configure :default @19:28:40.64 Elbow.reconfigure :limp @19:28:40.79 Forearm.reconfigure :loose @19:28:40.90 Forearm.seek 3 @19:28:42.27 Elbow.hold @19:28:42.41 Elbow.reconfigure :tight @19:28:42.65 SE.seek 14.11 Pucks below Top Plate @19:28:43.31 Forearm.force -20 @19:28:43.41 Forearm.seek 3 + 295 counts @19:28:43.88 Forearm.reconfigure :tight @19:28:43.98 Forearm.seek 3 + 800 counts @19:28:44.34 SE.seek 15.11 Pucks below Top Plate @19:28:44.52 PSR.select :air @19:28:44.70 PS.seek 2.5ml @19:28:45.07 Forearm.reconfigure :loose @19:28:45.12 Forearm.seek :retract @19:28:45.31 upsync @19:28:46.05 Elbow.seek CC @19:28:47.70 Elbow.reconfigure :loose @19:28:47.81 Forearm.seek CC @19:28:48.77 Hand.close @19:28:49.48 CC.seek :open @19:28:53.70 Forearm.seek :retract @19:28:54.68 Elbow.reconfigure :tight @19:28:54.73 Elbow.seek 3 @19:28:56.43 Elbow.reconfigure :limp @19:28:56.49 Forearm.seek 3 @19:28:57.61 Elbow.hold @19:28:57.69 Elbow.reconfigure :tight @19:28:57.74 Hand.open @19:28:59.05 Forearm.force -20 @19:28:59.10 Forearm.seek 3 + 319 counts @19:28:59.39 Forearm.reconfigure :tight @19:28:59.45 Forearm.seek 3 + 800 counts @19:28:59.81 SE.seek :down @19:29:00.95 Forearm.reconfigure :loose @19:29:01.00 Forearm.seek :retract @19:29:01.08 SE.stop @19:29:01.90
Starting finalPurge @19:29:06.05 delay 1 second @19:29:07.07 PRV.dial :waste2,avoiding: :ab2 @19:29:08.95 PTV.dial :PRVmixing @19:29:10.25 PSV.dial :mixloop @19:29:11.31 PS.seek :empty @19:29:21.13
Waiting >20 seconds for psFlush to relinquish FlushPuck @19:29:37.70 PSR.select :air @19:29:37.72 PSV.dial PSR @19:29:38.78 Pulling 0.5ml of PSR.flush @19:29:38.79 PSR.select :flush @19:29:38.91 PS.seek 0.5ml @19:29:44.95 delay 5 seconds @19:29:49.97 PSR.select :air @19:29:50.09 PS.seek 2.5ml @19:30:11.51 delay 1 second @19:30:12.53 PBV.dial :waste1 @19:30:12.92 PTV.dial :mixing @19:30:13.67 PSV.dial :mixloop @19:30:14.75 PS.seek :empty @19:30:41.11 PSV.dial PSR @19:30:42.15 PSR.select :flush @19:30:42.27 PS.seek 1ml @19:30:53.61 PSR.select :air @19:30:53.72 PS.seek 2.5ml @19:31:09.95 PSV.dial PTV @19:31:10.70 PTV.dial :puck @19:31:11.43 PBV.dial :waste1 @19:31:11.52 PS.seek 0ml @19:31:37.89 PSV.dial PSR @19:31:38.64 PSR.select :flush @19:31:38.76 PS.seek 0.5ml @19:31:44.85 PSR.select :air @19:31:44.97 PS.seek 2.5ml @19:32:06.70 PSV.dial PTV @19:32:07.45 PBV.dial PSV @19:32:08.03 PS.seek 0ml @19:32:34.48 PSV.dial PBV @19:32:34.94 PS.seek 2.5ml @19:33:01.32 PSV.dial :waste1 @19:33:02.19 PS.seek 0ml @19:33:28.54 PSV.dial PSR @19:33:28.99 PSR.select :air @19:33:29.02 PS.seek 2.5ml @19:33:55.42 PSV.dial PTV @19:33:56.15 PBV.dial PSV @19:33:56.24 PS.seek 0ml @19:34:22.64 PSV.dial PBV @19:34:23.09 PS.seek 2.5ml @19:34:49.51 PSV.dial :waste1 @19:34:50.38 PS.seek 0ml @19:35:16.73 PSV.dial PSR @19:35:17.18 PSR.select :flush @19:35:17.30 PS.seek 1ml @19:35:28.29 PSR.select :air @19:35:28.41 PS.seek 2.5ml @19:35:44.51 PSV.dial PTV @19:35:45.24 PTV.dial :puck @19:35:45.32 PBV.dial :waste1 @19:35:45.91 PS.seek 0ml @19:36:12.26 PSV.dial PSR @19:36:13.01 PSR.select :flush @19:36:13.13 PS.seek 0.5ml @19:36:19.07 PSR.select :air @19:36:19.19 PS.seek 2.5ml @19:36:40.76 PSV.dial PTV @19:36:41.51 PBV.dial PSV @19:36:42.09 PS.seek 0ml @19:37:08.45 PSV.dial PBV @19:37:08.90 PS.seek 2.5ml @19:37:35.32 PSV.dial :waste1 @19:37:36.06 PS.seek 0ml @19:38:02.48 PSV.dial PSR @19:38:02.95 PSR.select :air @19:38:02.97 PS.seek 2.5ml @19:38:29.29 PSV.dial PTV @19:38:30.41 PBV.dial PSV @19:38:30.56 PS.seek 0ml @19:38:56.91 PSV.dial PBV @19:38:57.37 PS.seek 2.5ml @19:39:23.79 PSV.dial :waste1 @19:39:24.53 PS.seek 0ml @19:39:50.86
Loading FlushPuck into Collection Clamp from Processing Clamp @19:39:51.14 Elbow.seek PC @19:39:53.67 Elbow.reconfigure :loose @19:39:53.78 Forearm.seek PC @19:39:54.87 Hand.close @19:39:55.59 PC.seek :open @19:40:00.09 Forearm.seek :retract @19:40:01.11 Elbow.reconfigure :tight @19:40:01.15 Elbow.seek CC @19:40:04.75 Elbow.reconfigure :loose @19:40:04.85 Forearm.reconfigure :tight @19:40:04.90 Forearm.seek CC @19:40:06.20 CC.seek :closed @19:40:10.18 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.92 @19:40:10.24 Hand.open @19:40:11.02 Forearm.reconfigure :loose @19:40:11.07 Forearm.seek :retract @19:40:12.06 Flushing the Collection Valve::Manifold @19:40:12.18 CSV.dial CSR @19:40:12.62 Pulling 4.999ml of CSR.flush @19:40:12.63 CSR.select :flush @19:40:12.74 CS.seek 5ml @19:40:26.45 delay 5 seconds @19:40:31.92 CSR.select :air @19:40:32.10 CS.reconfigure :fast @19:40:32.15 CS.seek 7.501ml @19:40:38.70 CSV.dial :waste @19:40:39.98 CS.reconfigure :default @19:40:40.03 CS.seek 0ml @19:41:00.01 Flushing lower manifold's waste1 port @19:41:00.14 CSV.dial :flush @19:41:00.58 CS.reconfigure :fast @19:41:00.63 CS.seek 5ml @19:41:12.95 delay 5 seconds @19:41:17.97 CSV.dial :air @19:41:18.48 CS.seek 7ml @19:41:23.98 CSV.dial CTV @19:41:24.02
CTV.dial CSV @19:41:25.43 CBV.dial :waste1 @19:41:26.21 CS.seek 0ml @19:41:42.89 Flushing lower manifold's waste2 port @19:41:43.01 CSV.dial :flush @19:41:43.75 CS.seek 5ml @19:41:55.98 delay 5 seconds @19:42:01.00 CSV.dial :air @19:42:01.51 CS.seek 6.999ml @19:42:07.07 CSV.dial CTV @19:42:07.10
CTV.dial CSV @19:42:07.56 CBV.dial :waste2 @19:42:08.61 CS.seek 0ml @19:42:25.39 Flushing lower manifold's waste2b port @19:42:25.51 CSV.dial :flush @19:42:26.25 CS.seek 5ml @19:42:38.57 delay 5 seconds @19:42:43.59 CSV.dial :air @19:42:44.10 CS.seek 7ml @19:42:49.57 CSV.dial CTV @19:42:49.60
CTV.dial CSV @19:42:50.04 CBV.dial :waste2b @19:42:51.08 CS.seek 0ml @19:43:07.67 Disinfecting external Intake with kill @19:43:07.79 CSV.dial CSR @19:43:08.23 Pulling 8ml of CSR.kill @19:43:08.24 CSR.select :kill @19:43:08.35 CS.seek 8ml @19:43:27.51 delay 5 seconds @19:43:32.53 CSR.select :air @19:43:32.65 CS.seek 10ml @19:43:38.52 CSV.dial CBV @19:43:38.55
CBV.dial CSV @19:43:39.60 CTV.dial :intake @19:43:40.37 Intake.select :open @19:43:40.48 CS.seek 2ml @19:43:59.51 Intake.select :close @19:43:59.61 CTV.dial :air @19:44:00.37 CS.reconfigure :default @19:44:00.42 CS.seek 0ml @19:44:06.51 CSV.dial CSR @19:44:07.52 Pulling 5ml of CSR.flush @19:44:07.53 CSR.select :flush @19:44:07.65 CS.seek 5ml @19:44:21.35 delay 5 seconds @19:44:26.37 CSR.select :air @19:44:26.49 CS.seek 10ml @19:44:40.16 CSV.dial CBV @19:44:41.30 CS.seek 0ml @19:45:07.70 Garaging FlushPuck in Collection Clamp @19:45:07.77 Elbow.reconfigure :tight @19:45:07.81 Elbow.seek CC @19:45:08.10 Elbow.reconfigure :loose @19:45:08.20 Forearm.seek CC @19:45:09.14 Hand.close @19:45:09.83 CC.seek :open @19:45:13.90 Forearm.seek :retract @19:45:14.93 Elbow.reconfigure :tight @19:45:14.97 Elbow.seek :garage @19:45:17.79 Elbow.reconfigure :loose @19:45:17.89 Forearm.reconfigure :tight @19:45:17.94 Forearm.seek :garage @19:45:18.99 Hand.open @19:45:19.77 Forearm.reconfigure :loose @19:45:19.81 Forearm.seek :retract @19:45:20.86 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 17:44:15 @19:45:20.92 Asleep after completing DA_WCR_120325 assays @19:45:20.93 GoodNews.email "{1 => 22,\n 2 => 22,\n 3 => 16,\n 4 => 6,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@19:43:38, 40% flow, 19.8C, 28% humidity, 14.6psia, 13.04V, 1.905A avg, 394.177Ah, 24.84W\nSampled 120.2ml",Subject: "Asleep after completing DA_WCR_120325 assays" @19:45:25.41 upsync + ESPwhich DA_WCR_121025.rb + fn=DA_WCR_121025.rb + IFS=: + set /home/esp/esp2/mission . + fullPath=/home/esp/esp2/mission/DA_WCR_121025.rb + [ -f /home/esp/esp2/mission/DA_WCR_121025.rb ] + realpath /home/esp/esp2/mission/DA_WCR_121025.rb + return 0 + scriptPath=/home/esp/esp2local/mission/DA_WCR_121025.rb + exec ruby /home/esp/esp2/lib/esp.rb /home/esp/esp2local/mission/DA_WCR_121025.rb @18:33:23.58UTC10-Dec-25
ESP2 v3.42 I2C Gateway v3.20 for core bus @18:33:26.26 Configuration for dennis 2/3/21 brent@mbari.org /home/esp/esp2/type/shallow/dennis/configure.rb @18:33:29.94 Polling Can every 10:00 @18:33:30.08 Cleanly exited at 18:33:08UTC10-Dec-25 @18:33:30.13 Loading /home/esp/esp2local/mission/DA_WCR_121025.rb mission @18:33:30.18 GoodNews.email "Starting DA_WCR_121025 w/pucks in tube 4\n{1 => 22,\n 2 => 22,\n 3 => 16,\n 4 => 6,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@18:33:29, 42% flow, 18.3C, 30% humidity, 14.5psia, 13.74V, 0.290A avg, 430.872Ah, 3.98W",Subject: "DA_WCR_121025 begun" @18:33:30.22
Preparing to sleep until + 00:00:05 @18:33:30.26 Asleep til + 00:00:05 @18:33:30.27 Continuing at 18:33 @18:33:30.55 Gate.power :core,:OFF @18:33:30.57 Gate.power :raw,:OFF @18:33:30.58 Shutdown @18:33:33.14 GoodNews.email "Then, in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121025.rb:14\n{1 => 22,\n 2 => 22,\n 3 => 16,\n 4 => 6,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@18:33:30, 42% flow, 18.3C, 30% humidity, 14.5psia, 13.74V, 0.290A avg, 430.872Ah, 3.98W",Subject: "Asleep until 18:33" @18:33:34.62 upsync @18:33:35.17
Powering core bus @18:33:37.01 upsync @18:33:40.65
Gate.power :raw,:ON @18:33:41.68 CS.configure :default @18:33:41.73 PS.configure :default @18:33:41.77 SS.configure :default @18:33:41.82 CC.configure :default @18:33:41.87 PC.configure :default @18:33:41.94 Forearm.configure :loose @18:33:41.99 Elbow.configure :loose @18:33:42.05 SC.configure :loose @18:33:42.10 SE.configure :default @18:33:42.15 CH.configure :default @18:33:42.18 PH.configure :default @18:33:42.22 All dwarf controllers running firmware version 3.85 @18:33:42.33 Valve.reset @18:33:42.61 Intake.select :close @18:33:42.63 Exhaust.select :close @18:33:42.64 CTV.dial :air @18:33:42.71 PSV.dial :air @18:33:42.85 CSV.dial :waste1 @18:33:42.88
Forearm.home.seek :retract @18:33:43.47 SSV.dial :spit,avoiding: :exhaust @18:33:43.67 Hand.close @18:33:43.72
Elbow.reconfigure :loose @18:33:43.78 Elbow.jog 1500 @18:33:43.85 PS.home.seek 0ml @18:33:44.25
Elbow.reconfigure :loose @18:33:44.30 Elbow.home.seek :clear @18:33:44.37 CS.reconfigure :fast @18:33:44.40 SS.home.seek 0ml @18:33:44.51 CS.home.seek 0ml @18:33:47.49
SE.home.seek :down @18:33:48.12 SC.reconfigure :tight @18:33:48.17 SC.home.seek :initial @18:33:52.96 PS.stop @18:34:04.13
SC.configure :limp @18:34:04.18 Forearm.seek :retract @18:34:04.25 Elbow.seek :hammer @18:34:05.28 Forearm.reconfigure :tight @18:34:05.33 Forearm.seek retracted @18:34:05.46 Forearm.coast @18:34:05.87 Arm::StretchError in MAIN -- Holding position retracted requires 37mA @18:34:05.89 Retry #1 of 5 @18:34:05.96 Hammering Forearm against inboard stop to recenter bearing races @18:34:06.05 Forearm.reconfigure :loose @18:34:06.10 Forearm.seek between 4 and retracted @18:34:06.76 Forearm.force 100 @18:34:07.36 Forearm.stop @18:34:07.78 Forearm.reconfigure :tight @18:34:07.83 Forearm.seek retracted @18:34:08.19 PC.home.seek :open @18:34:08.20
CC.home.seek :open @18:34:11.38 Phase [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] @18:34:11.39 Notice.email "At 18:34:11 began in tube 4: initialPurge; da [1000, 1000] #da and wsr are run simultanelously\n/home/esp/esp2local/mission/DA_WCR_121025.rb:14\n{1 => 22,\n 2 => 22,\n 3 => 16,\n 4 => 6,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@18:34:11, 43% flow, 18.3C, 30% humidity, 14.5psia, 13.68V, 0.259A avg, 430.877Ah, 3.54W",Subject: "Started DA_WCR_121025 phase initialPurge; da [1000, 1000] #da and wsr are run simultanelously" @18:34:11.41
Starting initialPurge @18:34:11.44 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:34:11.50 Hand.open @18:34:12.37 Elbow.reconfigure :tight @18:34:12.42 Elbow.seek FlushPuck::Garage @18:34:14.32 Elbow.reconfigure :loose @18:34:14.43 Forearm.reconfigure :loose @18:34:14.49 Forearm.seek :garage @18:34:15.44 Hand.close @18:34:15.49 upsync @18:34:16.36
Forearm.seek :retract @18:34:17.33 Elbow.reconfigure :tight @18:34:17.38 Elbow.seek CC @18:34:20.18 Elbow.reconfigure :loose @18:34:20.28 Forearm.reconfigure :tight @18:34:20.33 Forearm.seek CC @18:34:21.63 CC.seek :closed @18:34:25.64 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.98 @18:34:25.71 Hand.open @18:34:26.55 Forearm.reconfigure :loose @18:34:26.60 Forearm.seek :retract @18:34:27.59 Flushing the Collection Valve::Manifold @18:34:27.72 CSV.dial CSR @18:34:29.00 Pulling 5ml of CSR.flush @18:34:29.01 CSR.select :flush @18:34:29.12 CS.reconfigure :default @18:34:29.17 CS.seek 5ml @18:34:43.20 delay 5 seconds @18:34:48.22 CSR.select :air @18:34:48.39 CS.reconfigure :fast @18:34:48.43 CS.seek 7.5ml @18:34:55.04 CSV.dial :waste @18:34:56.33 CS.reconfigure :default @18:34:56.38 CS.seek 0ml @18:35:16.45 Purging external Intake @18:35:16.57 CSV.dial :flush @18:35:17.03 CS.reconfigure :fast @18:35:17.13 CS.seek 10ml @18:35:41.04 delay 5 seconds @18:35:46.06 CSV.dial CBV @18:35:46.11
CBV.dial CSV @18:35:46.84 CTV.dial :intake @18:35:47.62 Intake.select :open @18:35:47.73 CS.seek 0ml @18:36:11.57 Intake.select :close @18:36:11.68 CTV.dial :air @18:36:12.45 Flushing upper manifold @18:36:12.57 CSV.dial :flush @18:36:13.31 CS.seek 5ml @18:36:25.79 delay 5 seconds @18:36:30.81 CSV.dial :air @18:36:31.31 CS.seek 7ml @18:36:36.86 CSV.dial CBV @18:36:36.88
CBV.dial CSV @18:36:37.90 CTV.dial :air @18:36:37.99 CS.seek 0ml @18:36:54.82 delay 7 seconds @18:37:01.85 CS.seek 3ml @18:37:09.66 CSV.dial :waste @18:37:10.13 CS.seek 0ml @18:37:17.96 CSV.dial :flush @18:37:18.41 CS.seek 3ml @18:37:26.23 delay 5 seconds @18:37:31.25 CSV.dial :air @18:37:31.87 CS.seek 5ml @18:37:37.42 CSV.dial :air @18:37:37.50 CS.seek 0ml @18:37:49.82 delay 7 seconds @18:37:56.85 CS.seek 3ml @18:38:04.66 CSV.dial :waste @18:38:05.39 CS.seek 0ml @18:38:13.14 Garaging FlushPuck in Collection Clamp @18:38:13.21 Elbow.reconfigure :tight @18:38:13.25 Elbow.seek CC @18:38:13.37 Elbow.reconfigure :loose @18:38:13.47 Forearm.seek CC @18:38:14.51 Hand.close @18:38:15.23 CC.seek :open @18:38:19.75 Forearm.seek :retract @18:38:20.76 Elbow.reconfigure :tight @18:38:20.81 Elbow.seek :garage @18:38:23.69 Elbow.reconfigure :loose @18:38:23.79 Forearm.reconfigure :tight @18:38:23.84 Forearm.seek :garage @18:38:24.78 Hand.open @18:38:25.54 Forearm.reconfigure :loose @18:38:25.58 Forearm.seek :retract @18:38:26.64 da sampling at most 1000ml, wcr at most 1000ml @18:38:26.67 DAfans.email "Started da in tube 4 at 18:38:26UTC10-Dec-25\n{1 => 22,\n 2 => 22,\n 3 => 16,\n 4 => 6,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@18:34:11, 43% flow, 18.3C, 30% humidity, 14.5psia, 13.68V, 0.259A avg, 430.877Ah, 3.54W",Subject: "da sampling at most 1000ml, wcr at most 1000ml" @18:38:26.70
Loading sh1 puck from tube 4 into Collection Clamp @18:38:27.24 Elbow.reconfigure :tight @18:38:27.29 Elbow.seek 4 @18:38:29.16 Forearm.reconfigure :tight @18:38:29.21 Forearm.seek 4 + 800 counts @18:38:30.25 SE.reconfigure :pick @18:38:30.33 SE.seek :beamBreak @18:38:30.81 upsync @18:38:32.80
SE.configure :default @18:38:32.97 Elbow.seek 4 @18:38:33.16 Forearm.seek 4 + 300 counts @18:38:33.41 Forearm.reconfigure :loose @18:38:33.46 Hand.close @18:38:34.24 SE.seek :down @18:38:34.45
Forearm.seek :retract @18:38:35.51 Elbow.seek CC @18:38:36.58 SE.stop @18:38:37.54
Elbow.reconfigure :loose @18:38:37.64 Forearm.reconfigure :tight @18:38:37.68 Forearm.seek CC @18:38:39.04 CC.seek :closed @18:38:43.07 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.00 @18:38:43.08 Hand.open @18:38:43.91 Forearm.reconfigure :loose @18:38:43.96 Forearm.seek :retract @18:38:44.99 collectSample 1000ml w/bubble point of 24psi @18:38:45.01 Priming 7ml @18:38:45.02 Intake.select :close @18:38:45.03 Exhaust.select :close @18:38:45.04 SS.hold @18:38:45.25 Calibrating pressure and temperature sensors @18:38:45.28 CTV.dial :air @18:38:45.29 SSV.dial :spit,avoiding: :exhaust @18:38:45.38 SS.reconfigure :fast @18:38:45.43 SS.seek 5ml @18:38:48.57 SSV.dial CBV @18:38:48.59
CBV.dial SSV @18:38:49.97 delay 3.8 seconds @18:38:53.90 Sensor limits: Intake max 89.3psia; Outlet max 107.7psia @18:38:53.91 SSV.dial :spit @18:38:55.26 SS.seek 0ml @18:38:58.12 SSV.dial CBV @18:38:59.49 CTV.dial :intake @18:39:00.26 SSV.dial CBV @18:39:00.30
CBV.dial SSV @18:39:00.43 Allowing 40s for 7.0ml of remaining 7.0ml @18:39:00.44 Intake.select :open @18:39:02.60 Intake @14.3psia, vacuum from 8.8 to 11.8psi @18:39:02.62 SS.reconfigure :inhaling 2.17ml/s @18:39:02.67 SS.seek 7ml @18:39:08.12 Stroke completed -- waiting for pressure to equalize ... @18:39:08.13 SS.reconfigure :exhale @18:39:08.18 SS.hold @18:39:10.79 Intake.select :close @18:39:10.95 SSV.dial :exhaust @18:39:11.74 Exhaust.select :open @18:39:11.85 SS.seek 0ml @18:39:16.16 Exhaust.select :close @18:39:16.27 Sampling 1000.0ml @18:39:16.28 Intake.select :close @18:39:16.29 Exhaust.select :close @18:39:16.30 SS.reconfigure :default @18:39:16.35 SS.hold @18:39:16.55 CTV.dial :intake @18:39:16.73 SSV.dial CBV @18:39:16.76
CBV.dial SSV @18:39:17.55 Allowing 130s for 25.0ml of remaining 1000.0ml @18:39:17.56 Intake.select :open @18:39:19.73 Intake @13.8psia, vacuum from 8.3 to 11.3psi @18:39:19.75 SS.reconfigure :inhaling 2.17ml/s @18:39:19.79 SS.seek 25ml @18:39:33.98 Vacuum too high -- waiting up to 40 seconds @18:39:33.99 SS.reconfigure :exhale @18:39:34.04 SS.hold @18:39:34.51 Intake @8.8psia, vacuum from 3.3 to 6.3psi @18:39:34.53 SS.reconfigure :inhaling 1.95ml/s @18:39:34.60 SS.seek 25ml @18:39:34.64 Vacuum too high -- waiting up to 40 seconds @18:39:34.65 SS.reconfigure :exhale @18:39:34.70 SS.hold @18:39:43.34 Intake @11.7psia, vacuum from 6.2 to 9.2psi @18:39:43.36 SS.reconfigure :inhaling 1.77ml/s @18:39:43.41 SS.seek 25ml @18:39:44.09 Stroke completed -- waiting for pressure to equalize ... @18:39:44.10 SS.reconfigure :exhale @18:39:44.15 SS.hold @18:39:44.87 Intake.select :close @18:39:45.03 SSV.dial :exhaust @18:39:45.78 Exhaust.select :open @18:39:45.90 SS.seek 0ml @18:39:54.64 Exhaust.select :close @18:39:54.75 SSV.dial CBV @18:39:54.79
CBV.dial SSV @18:39:55.55 Allowing 130s for 25.0ml of remaining 975.0ml @18:39:55.56 Intake.select :open @18:39:57.73 Intake @13.4psia, vacuum from 7.9 to 10.9psi @18:39:57.74 SS.reconfigure :inhaling 1.77ml/s @18:39:57.79 SS.seek 25ml @18:40:10.54 Vacuum too high -- waiting up to 40 seconds @18:40:10.55 SS.reconfigure :exhale @18:40:10.60 SS.hold @18:40:11.27 Intake @8.8psia, vacuum from 3.3 to 6.3psi @18:40:11.29 SS.reconfigure :inhaling 1.59ml/s @18:40:11.33 SS.seek 25ml @18:40:11.42 Vacuum too high -- waiting up to 40 seconds @18:40:11.43 SS.reconfigure :exhale @18:40:11.49 SS.hold @18:40:16.31 Intake @10.9psia, vacuum from 5.4 to 8.4psi @18:40:16.32 SS.reconfigure :inhaling 1.43ml/s @18:40:16.37 SS.seek 25ml @18:40:22.62 Stroke completed -- waiting for pressure to equalize ... @18:40:22.63 SS.reconfigure :exhale @18:40:22.68 SS.hold @18:40:25.58 Intake.select :close @18:40:25.74 SSV.dial :exhaust @18:40:26.52 Exhaust.select :open @18:40:26.63 SS.seek 0ml @18:40:35.83 Exhaust.select :close @18:40:35.94 SSV.dial CBV @18:40:35.96
CBV.dial SSV @18:40:36.77 Allowing 130s for 25.0ml of remaining 950.0ml @18:40:36.78 Intake.select :open @18:40:38.94 Intake @13.2psia, vacuum from 7.7 to 10.7psi @18:40:38.96 SS.reconfigure :inhaling 1.43ml/s @18:40:39.01 SS.seek 25ml @18:40:48.29 Vacuum too high -- waiting up to 40 seconds @18:40:48.30 SS.reconfigure :exhale @18:40:48.35 SS.hold @18:40:48.86 Intake @9.5psia, vacuum from 4.0 to 7.0psi @18:40:48.87 SS.reconfigure :inhaling 1.29ml/s @18:40:48.92 SS.seek 25ml @18:40:57.88 Vacuum too high -- waiting up to 40 seconds @18:40:57.89 SS.reconfigure :exhale @18:40:57.94 SS.hold @18:41:01.05 Intake @10.7psia, vacuum from 5.2 to 8.2psi @18:41:01.07 SS.reconfigure :inhaling 1.16ml/s @18:41:01.12 SS.seek 25ml @18:41:05.03 Stroke completed -- waiting for pressure to equalize ... @18:41:05.04 SS.reconfigure :exhale @18:41:05.08 SS.hold @18:41:07.46 Intake.select :close @18:41:07.61 SSV.dial :exhaust @18:41:08.39 Exhaust.select :open @18:41:08.50 SS.seek 0ml @18:41:17.72 Exhaust.select :close @18:41:17.82 SSV.dial CBV @18:41:18.28
CBV.dial SSV @18:41:19.08 Allowing 130s for 25.0ml of remaining 925.0ml @18:41:19.09 Intake.select :open @18:41:21.26 Intake @13.3psia, vacuum from 7.8 to 10.8psi @18:41:21.28 SS.reconfigure :inhaling 1.16ml/s @18:41:21.32 SS.seek 25ml @18:41:44.45 Stroke completed -- waiting for pressure to equalize ... @18:41:44.46 SS.reconfigure :exhale @18:41:44.50 SS.hold @18:41:48.67 Intake.select :close @18:41:48.82 SSV.dial :exhaust @18:41:49.61 Exhaust.select :open @18:41:49.73 SS.seek 0ml @18:41:58.61 Exhaust.select :close @18:41:58.72 SSV.dial CBV @18:41:58.74
CBV.dial SSV @18:41:59.53 Allowing 130s for 25.0ml of remaining 900.0ml @18:41:59.54 Intake.select :open @18:42:01.70 Intake @12.7psia, vacuum from 7.2 to 10.2psi @18:42:01.72 SS.reconfigure :inhaling 1.16ml/s @18:42:01.77 SS.seek 25ml @18:42:17.60 Vacuum too high -- waiting up to 40 seconds @18:42:17.61 SS.reconfigure :exhale @18:42:17.66 SS.hold @18:42:18.10 Intake @9.5psia, vacuum from 4.0 to 7.0psi @18:42:18.12 SS.reconfigure :inhaling 1.04ml/s @18:42:18.17 SS.seek 25ml @18:42:21.47 Vacuum too high -- waiting up to 40 seconds @18:42:21.48 SS.reconfigure :exhale @18:42:21.54 SS.hold @18:42:23.24 Intake @8.8psia, vacuum from 3.3 to 6.3psi @18:42:23.26 SS.reconfigure :inhaling 0.94ml/s @18:42:23.31 SS.seek 25ml @18:42:29.29 Stroke completed -- waiting for pressure to equalize ... @18:42:29.30 SS.reconfigure :exhale @18:42:29.35 SS.hold @18:42:30.07 Intake.select :close @18:42:30.22 SSV.dial :exhaust @18:42:31.01 Exhaust.select :open @18:42:31.12 SS.seek 0ml @18:42:40.03 Exhaust.select :close @18:42:40.14 SSV.dial CBV @18:42:40.16
CBV.dial SSV @18:42:40.98 Allowing 130s for 25.0ml of remaining 875.0ml @18:42:40.99 Intake.select :open @18:42:43.16 Intake @12.6psia, vacuum from 7.1 to 10.1psi @18:42:43.17 SS.reconfigure :inhaling 0.94ml/s @18:42:43.22 SS.seek 25ml @18:43:02.44 Vacuum too high -- waiting up to 40 seconds @18:43:02.45 SS.reconfigure :exhale @18:43:02.51 SS.hold @18:43:02.93 Intake @8.7psia, vacuum from 3.2 to 6.2psi @18:43:02.94 SS.reconfigure :inhaling 0.85ml/s @18:43:02.99 SS.seek 25ml @18:43:12.74 Stroke completed -- waiting for pressure to equalize ... @18:43:12.75 SS.reconfigure :exhale @18:43:12.80 SS.hold @18:43:15.30 Intake.select :close @18:43:15.45 SSV.dial :exhaust @18:43:16.21 Exhaust.select :open @18:43:16.32 SS.seek 0ml @18:43:25.23 Exhaust.select :close @18:43:25.34 SSV.dial CBV @18:43:25.36
CBV.dial SSV @18:43:26.22 Allowing 130s for 25.0ml of remaining 850.0ml @18:43:26.24 Intake.select :open @18:43:28.41 Intake @12.8psia, vacuum from 7.3 to 10.3psi @18:43:28.43 SS.reconfigure :inhaling 0.85ml/s @18:43:28.48 SS.seek 25ml @18:43:57.25 Vacuum too high -- waiting up to 40 seconds @18:43:57.26 SS.reconfigure :exhale @18:43:57.32 SS.hold @18:43:58.01 Intake @10.4psia, vacuum from 4.9 to 7.9psi @18:43:58.03 SS.reconfigure :inhaling 0.77ml/s @18:43:58.08 SS.seek 25ml @18:44:00.27 Stroke completed -- waiting for pressure to equalize ... @18:44:00.28 SS.reconfigure :exhale @18:44:00.33 SS.hold @18:44:02.56 Intake.select :close @18:44:02.72 SSV.dial :exhaust @18:44:03.48 Exhaust.select :open @18:44:03.59 SS.seek 0ml @18:44:12.85 Exhaust.select :close @18:44:12.96 SSV.dial CBV @18:44:12.98
CBV.dial SSV @18:44:13.78 Allowing 130s for 25.0ml of remaining 825.0ml @18:44:13.79 Intake.select :open @18:44:15.95 Intake @13.1psia, vacuum from 7.6 to 10.6psi @18:44:15.97 SS.reconfigure :inhaling 0.77ml/s @18:44:16.02 SS.seek 25ml @18:44:49.98 Stroke completed -- waiting for pressure to equalize ... @18:44:49.99 SS.reconfigure :exhale @18:44:50.03 SS.hold @18:44:54.09 Intake.select :close @18:44:54.24 SSV.dial :exhaust @18:44:55.03 Exhaust.select :open @18:44:55.15 SS.seek 0ml @18:45:04.45 Exhaust.select :close @18:45:04.56 SSV.dial CBV @18:45:04.58
CBV.dial SSV @18:45:05.37 Allowing 130s for 25.0ml of remaining 800.0ml @18:45:05.38 Intake.select :open @18:45:07.54 Intake @13.6psia, vacuum from 8.1 to 11.1psi @18:45:07.56 SS.reconfigure :inhaling 0.77ml/s @18:45:07.61 SS.seek 25ml @18:45:30.47 Vacuum too high -- waiting up to 40 seconds @18:45:30.48 SS.reconfigure :exhale @18:45:30.53 SS.hold @18:45:30.95 Intake @10.9psia, vacuum from 5.4 to 8.4psi @18:45:30.97 SS.reconfigure :inhaling 0.70ml/s @18:45:31.01 SS.seek 25ml @18:45:38.63 Vacuum too high -- waiting up to 40 seconds @18:45:38.65 SS.reconfigure :exhale @18:45:38.70 SS.hold @18:45:40.80 Intake @10.5psia, vacuum from 5.0 to 8.0psi @18:45:40.82 SS.reconfigure :inhaling 0.62ml/s @18:45:40.87 SS.seek 25ml @18:45:47.18 Stroke completed -- waiting for pressure to equalize ... @18:45:47.19 SS.reconfigure :exhale @18:45:47.23 SS.hold @18:45:49.70 Intake.select :close @18:45:49.85 SSV.dial :exhaust @18:45:50.63 Exhaust.select :open @18:45:50.74 SS.seek 0ml @18:45:59.91 Exhaust.select :close @18:46:00.02 SSV.dial CBV @18:46:00.04
CBV.dial SSV @18:46:00.84 Allowing 130s for 25.0ml of remaining 775.0ml @18:46:00.85 Intake.select :open @18:46:03.02 Intake @13.1psia, vacuum from 7.6 to 10.6psi @18:46:03.03 SS.reconfigure :inhaling 0.62ml/s @18:46:03.08 SS.seek 25ml @18:46:44.60 Stroke completed -- waiting for pressure to equalize ... @18:46:44.61 SS.reconfigure :exhale @18:46:44.65 SS.hold @18:46:48.62 Intake.select :close @18:46:48.78 SSV.dial :exhaust @18:46:49.54 Exhaust.select :open @18:46:49.65 SS.seek 0ml @18:46:58.91 Exhaust.select :close @18:46:59.02 SSV.dial CBV @18:46:59.05
CBV.dial SSV @18:46:59.84 Allowing 130s for 25.0ml of remaining 750.0ml @18:46:59.85 Intake.select :open @18:47:02.01 Intake @13.9psia, vacuum from 8.4 to 11.4psi @18:47:02.03 SS.reconfigure :inhaling 0.62ml/s @18:47:02.08 SS.seek 25ml @18:47:25.81 Vacuum too high -- waiting up to 40 seconds @18:47:25.82 SS.reconfigure :exhale @18:47:25.87 SS.hold @18:47:26.87 Intake @11.7psia, vacuum from 6.2 to 9.2psi @18:47:26.89 SS.reconfigure :inhaling 0.56ml/s @18:47:26.94 SS.seek 25ml @18:47:41.87 Vacuum too high -- waiting up to 40 seconds @18:47:41.89 SS.reconfigure :exhale @18:47:41.94 SS.hold @18:47:46.89 Intake @12.1psia, vacuum from 6.6 to 9.6psi @18:47:46.91 SS.reconfigure :inhaling 0.51ml/s @18:47:46.95 SS.seek 25ml @18:47:52.14 Stroke completed -- waiting for pressure to equalize ... @18:47:52.15 SS.reconfigure :exhale @18:47:52.19 SS.hold @18:47:56.79 Intake.select :close @18:47:56.95 SSV.dial :exhaust @18:47:57.72 Exhaust.select :open @18:47:57.83 SS.seek 0ml @18:48:06.68 Exhaust.select :close @18:48:06.79 SSV.dial CBV @18:48:06.81
CBV.dial SSV @18:48:07.60 Allowing 130s for 25.0ml of remaining 725.0ml @18:48:07.61 Intake.select :open @18:48:09.78 Intake @14.1psia, vacuum from 8.6 to 11.6psi @18:48:09.80 SS.reconfigure :inhaling 0.51ml/s @18:48:09.85 SS.seek 25ml @18:49:00.21 Stroke completed -- waiting for pressure to equalize ... @18:49:00.22 SS.reconfigure :exhale @18:49:00.26 SS.hold @18:49:07.64 Intake.select :close @18:49:07.79 SSV.dial :exhaust @18:49:08.57 Exhaust.select :open @18:49:08.68 SS.seek 0ml @18:49:17.88 Exhaust.select :close @18:49:17.99 SSV.dial CBV @18:49:18.01
CBV.dial SSV @18:49:18.80 Allowing 130s for 25.0ml of remaining 700.0ml @18:49:18.81 Intake.select :open @18:49:20.98 Intake @14.2psia, vacuum from 8.7 to 11.7psi @18:49:20.99 SS.reconfigure :inhaling 0.51ml/s @18:49:21.04 SS.seek 25ml @18:50:10.25 Vacuum too high -- waiting up to 40 seconds @18:50:10.38 SS.reconfigure :exhale @18:50:10.43 SS.hold @18:50:12.13 Intake @12.2psia, vacuum from 6.7 to 9.7psi @18:50:12.14 SS.reconfigure :inhaling 0.46ml/s @18:50:12.19 SS.seek 25ml @18:50:14.19 Stroke completed -- waiting for pressure to equalize ... @18:50:14.20 SS.reconfigure :exhale @18:50:14.24 SS.hold @18:50:20.75 Intake.select :close @18:50:20.90 SSV.dial :exhaust @18:50:21.68 Exhaust.select :open @18:50:21.79 SS.seek 0ml @18:50:31.19 Exhaust.select :close @18:50:31.30 SSV.dial CBV @18:50:31.32
CBV.dial SSV @18:50:32.14 Allowing 130s for 25.0ml of remaining 675.0ml @18:50:32.15 Intake.select :open @18:50:34.31 Intake @14.2psia, vacuum from 8.7 to 11.7psi @18:50:34.33 SS.reconfigure :inhaling 0.46ml/s @18:50:34.38 SS.seek 25ml @18:51:29.48 Stroke completed -- waiting for pressure to equalize ... @18:51:29.49 SS.reconfigure :exhale @18:51:29.54 SS.hold @18:51:37.23 Intake.select :close @18:51:37.38 SSV.dial :exhaust @18:51:38.16 Exhaust.select :open @18:51:38.27 SS.seek 0ml @18:51:47.53 Exhaust.select :close @18:51:47.64 SSV.dial CBV @18:51:47.68
CBV.dial SSV @18:51:48.44 Allowing 130s for 25.0ml of remaining 650.0ml @18:51:48.45 Intake.select :open @18:51:50.62 Intake @14.1psia, vacuum from 8.6 to 11.6psi @18:51:50.64 SS.reconfigure :inhaling 0.46ml/s @18:51:50.68 SS.seek 25ml @18:52:45.73 Stroke completed -- waiting for pressure to equalize ... @18:52:45.74 SS.reconfigure :exhale @18:52:45.79 SS.hold @18:52:55.56 Intake.select :close @18:52:55.72 SSV.dial :exhaust @18:52:56.78 Exhaust.select :open @18:52:56.89 SS.seek 0ml @18:53:06.14 Exhaust.select :close @18:53:06.25 SSV.dial CBV @18:53:06.28
CBV.dial SSV @18:53:07.08 Allowing 130s for 25.0ml of remaining 625.0ml @18:53:07.09 Intake.select :open @18:53:09.25 Intake @14.4psia, vacuum from 8.9 to 11.9psi @18:53:09.27 SS.reconfigure :inhaling 0.46ml/s @18:53:09.32 SS.seek 25ml @18:53:55.77 Vacuum too high -- waiting up to 40 seconds @18:53:55.78 SS.reconfigure :exhale @18:53:55.84 SS.hold @18:54:00.10 Intake @14.1psia, vacuum from 8.6 to 11.6psi @18:54:00.12 SS.reconfigure :inhaling 0.42ml/s @18:54:00.17 SS.seek 25ml @18:54:10.29 Stroke completed -- waiting for pressure to equalize ... @18:54:10.30 SS.reconfigure :exhale @18:54:10.35 SS.hold @18:54:17.87 Intake.select :close @18:54:18.03 SSV.dial :exhaust @18:54:18.80 Exhaust.select :open @18:54:18.91 SS.seek 0ml @18:54:27.79 Exhaust.select :close @18:54:27.90 SSV.dial CBV @18:54:27.92
CBV.dial SSV @18:54:28.73 Allowing 130s for 25.0ml of remaining 600.0ml @18:54:28.74 Intake.select :open @18:54:30.91 Intake @14.9psia, vacuum from 9.4 to 12.4psi @18:54:30.92 SS.reconfigure :inhaling 0.42ml/s @18:54:30.97 SS.seek 25ml @18:55:31.62 Stroke completed -- waiting for pressure to equalize ... @18:55:31.63 SS.reconfigure :exhale @18:55:31.68 SS.hold @18:55:38.17 Intake.select :close @18:55:38.32 SSV.dial :exhaust @18:55:39.09 Exhaust.select :open @18:55:39.20 SS.seek 0ml @18:55:48.11 Exhaust.select :close @18:55:48.21 SSV.dial CBV @18:55:48.24
CBV.dial SSV @18:55:49.11 Allowing 130s for 25.0ml of remaining 575.0ml @18:55:49.12 Intake.select :open @18:55:51.29 Intake @14.8psia, vacuum from 9.3 to 12.3psi @18:55:51.30 SS.reconfigure :inhaling 0.42ml/s @18:55:51.35 SS.seek 25ml @18:56:52.13 Stroke completed -- waiting for pressure to equalize ... @18:56:52.15 SS.reconfigure :exhale @18:56:52.19 SS.hold @18:57:03.34 Intake.select :close @18:57:03.49 SSV.dial :exhaust @18:57:04.26 Exhaust.select :open @18:57:04.37 SS.seek 0ml @18:57:13.74 Exhaust.select :close @18:57:13.86 SSV.dial CBV @18:57:13.88
CBV.dial SSV @18:57:14.68 Allowing 130s for 25.0ml of remaining 550.0ml @18:57:14.69 Intake.select :open @18:57:16.86 Intake @14.8psia, vacuum from 9.3 to 12.3psi @18:57:16.87 SS.reconfigure :inhaling 0.42ml/s @18:57:16.92 SS.seek 25ml @18:58:17.71 Stroke completed -- waiting for pressure to equalize ... @18:58:17.72 SS.reconfigure :exhale @18:58:17.77 SS.hold @18:58:30.86 Intake.select :close @18:58:31.01 SSV.dial :exhaust @18:58:31.77 Exhaust.select :open @18:58:31.88 SS.seek 0ml @18:58:41.26 Exhaust.select :close @18:58:41.37 SSV.dial CBV @18:58:41.39
CBV.dial SSV @18:58:42.18 Allowing 130s for 25.0ml of remaining 525.0ml @18:58:42.19 Intake.select :open @18:58:44.35 Intake @15.3psia, vacuum from 9.8 to 12.8psi @18:58:44.37 SS.reconfigure :inhaling 0.42ml/s @18:58:44.42 SS.seek 25ml @18:59:25.17 Vacuum too high -- waiting up to 40 seconds @18:59:25.18 SS.reconfigure :exhale @18:59:25.23 SS.hold @18:59:30.98 Intake @14.9psia, vacuum from 9.4 to 12.4psi @18:59:30.99 SS.reconfigure :inhaling 0.38ml/s @18:59:31.04 SS.seek 25ml @18:59:54.02 Stroke completed -- waiting for pressure to equalize ... @18:59:54.03 SS.reconfigure :exhale @18:59:54.08 SS.hold @19:00:06.75 Intake.select :close @19:00:06.91 SSV.dial :exhaust @19:00:07.68 Exhaust.select :open @19:00:07.79 SS.seek 0ml @19:00:17.15 Exhaust.select :close @19:00:17.25 SSV.dial CBV @19:00:17.28
CBV.dial SSV @19:00:18.07 Allowing 130s for 25.0ml of remaining 500.0ml @19:00:18.08 Intake.select :open @19:00:20.25 Intake @15.3psia, vacuum from 9.8 to 12.8psi @19:00:20.27 SS.reconfigure :inhaling 0.38ml/s @19:00:20.31 SS.seek 25ml @19:01:17.92 Vacuum too high -- waiting up to 40 seconds @19:01:17.93 SS.reconfigure :exhale @19:01:17.98 SS.hold @19:01:24.02 Intake @13.9psia, vacuum from 8.4 to 11.4psi @19:01:24.04 SS.reconfigure :inhaling 0.33ml/s @19:01:24.08 SS.seek 25ml @19:01:36.64 Stroke completed -- waiting for pressure to equalize ... @19:01:36.65 SS.reconfigure :exhale @19:01:36.70 SS.hold @19:01:51.41 Intake.select :close @19:01:51.56 SSV.dial :exhaust @19:01:52.33 Exhaust.select :open @19:01:52.44 SS.seek 0ml @19:02:01.83 Exhaust.select :close @19:02:01.94 SSV.dial CBV @19:02:01.99
CBV.dial SSV @19:02:02.74 Allowing 130s for 25.0ml of remaining 475.0ml @19:02:02.75 Intake.select :open @19:02:04.91 Intake @15.7psia, vacuum from 10.0 to 13.0psi @19:02:04.93 SS.reconfigure :inhaling 0.33ml/s @19:02:04.98 SS.seek 25ml @19:03:03.04 Vacuum too high -- waiting up to 40 seconds @19:03:03.06 SS.reconfigure :exhale @19:03:03.11 SS.hold @19:03:08.84 Intake @14.4psia, vacuum from 8.9 to 11.9psi @19:03:08.85 SS.reconfigure :inhaling 0.30ml/s @19:03:08.90 SS.seek 25ml @19:03:30.27 Stroke completed -- waiting for pressure to equalize ... @19:03:30.28 SS.reconfigure :exhale @19:03:30.32 SS.hold @19:03:54.74 Intake.select :close @19:03:54.89 SSV.dial :exhaust @19:03:55.66 Exhaust.select :open @19:03:55.78 SS.seek 0ml @19:04:05.30 Exhaust.select :close @19:04:05.41 SSV.dial CBV @19:04:05.43
CBV.dial SSV @19:04:06.24 Allowing 130s for 25.0ml of remaining 450.0ml @19:04:06.25 Intake.select :open @19:04:08.41 Intake @15.8psia, vacuum from 10.0 to 13.0psi @19:04:08.43 SS.reconfigure :inhaling 0.30ml/s @19:04:08.48 SS.seek 25ml @19:05:09.32 Vacuum too high -- waiting up to 40 seconds @19:05:09.33 SS.reconfigure :exhale @19:05:09.38 SS.hold @19:05:16.06 Intake @14.7psia, vacuum from 9.2 to 12.2psi @19:05:16.08 SS.reconfigure :inhaling 0.28ml/s @19:05:16.13 SS.seek 25ml @19:05:43.94 Stroke completed -- waiting for pressure to equalize ... @19:05:43.95 SS.reconfigure :exhale @19:05:43.99 SS.hold @19:06:04.29 Intake.select :close @19:06:04.44 SSV.dial :exhaust @19:06:05.23 Exhaust.select :open @19:06:05.34 SS.seek 0ml @19:06:14.70 Exhaust.select :close @19:06:14.81 SSV.dial CBV @19:06:14.83
CBV.dial SSV @19:06:16.69 Allowing 130s for 25.0ml of remaining 425.0ml @19:06:16.70 Intake.select :open @19:06:18.86 Intake @15.9psia, vacuum from 10.0 to 13.0psi @19:06:18.88 SS.reconfigure :inhaling 0.28ml/s @19:06:18.93 SS.seek 25ml @19:07:25.50 Vacuum too high -- waiting up to 40 seconds @19:07:25.52 SS.reconfigure :exhale @19:07:25.57 SS.hold @19:07:36.83 Intake @15.4psia, vacuum from 9.9 to 12.9psi @19:07:36.84 SS.reconfigure :inhaling 0.25ml/s @19:07:36.89 SS.seek 25ml @19:08:10.60 Stroke completed -- waiting for pressure to equalize ... @19:08:10.61 SS.reconfigure :exhale @19:08:10.66 SS.hold @19:08:26.71 SS.reconfigure :default @19:08:26.75 SS.hold @19:08:26.82 Intake.select :close @19:08:26.98 ShallowSampler::Clogged in MAIN -- Filtering too Slowly after 600.0ml @19:08:26.99 SSV.dial :exhaust @19:08:27.75 Exhaust.select :open @19:08:27.86 SS.reconfigure :exhale @19:08:27.91 SS.seek 0ml @19:08:37.39 Exhaust.select :close @19:08:37.50 Sampled 600.0ml @19:08:37.51 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @19:08:37.52 Intake.select :close @19:08:37.59 CSV.dial :waste @19:08:37.75 SSV.dial CBV,avoiding: :exhaust @19:08:37.78 CS.seek 0ml @19:08:37.81
CBV.dial SSV @19:08:37.94 CSV.dial :air @19:08:38.69 CS.seek 10ml @19:08:39.75
CTV.dial :air @19:08:40.53 delay 15 seconds @19:08:55.55 SSV.dial :spit,avoiding: :exhaust @19:08:56.89 CTV.dial CSV,avoiding: :intake @19:08:56.94
SS.seek 0ml @19:08:57.04 SSV.dial CBV,avoiding: :exhaust @19:09:02.98 SSV.dial between CBV and plug @19:09:03.48 CBV.dial between SSV and waste2 @19:09:03.88 CSV.dial between air and CTV @19:09:04.20 CS.seek 8.033ml @19:09:09.61 CTV.dial CSV,avoiding: :air @19:09:09.64
CSV.dial CTV @19:09:09.97 CS.reconfigure :pressurize @19:09:10.45 CS.seek 0ml @19:09:28.31 BubblePoint: Intake @24.0psia, Outlet @0.8psia @19:09:28.32 CBV.dial SSV @19:09:28.68 SS.reconfigure :default @19:09:28.72 SS.seek 10ml @19:09:34.20 delay 15 seconds @19:09:49.33 Evacuated: Intake @23.8psia, Outlet @22.3psia @19:09:49.35 SSV.dial :spit,avoiding: :exhaust @19:09:50.42 SS.reconfigure :exhale @19:09:50.47 SS.seek 0ml @19:09:55.66 SS.reconfigure :fast @19:09:55.71 SS.seek 5ml @19:09:58.28 SSV.dial CBV,avoiding: :exhaust @19:09:58.31
CBV.dial SSV @19:09:59.70 CTV.dial :air,avoiding: :intake @19:10:01.08 CSV.dial CTV @19:10:01.13
SS.seek 10ml @19:10:02.25 CSV.dial :air @19:10:02.70 CS.reconfigure :fast @19:10:02.75 CS.seek 0ml @19:10:03.64
SSV.dial :spit,avoiding: :exhaust @19:10:04.98 SS.reconfigure :exhale @19:10:05.03 SS.seek 0ml @19:10:06.52 CS.stop @19:10:09.96
SS.reconfigure :fast @19:10:10.00 SS.seek 5ml @19:10:13.00 SSV.dial CBV,avoiding: :exhaust @19:10:13.02
CBV.dial SSV @19:10:15.43 SSV.dial :spit,avoiding: :exhaust @19:10:16.76 SS.reconfigure :default @19:10:16.81 SS.seek 0ml @19:10:19.81 SS.stop @19:10:19.85 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @19:10:19.86 Intake.select :close @19:10:19.93 CSV.dial :waste @19:10:20.06 SSV.dial CBV,avoiding: :exhaust @19:10:20.08
CBV.dial SSV @19:10:20.69 CS.seek 0ml @19:10:20.76 CSV.dial :air @19:10:21.47
CTV.dial :air @19:10:21.54 CS.seek 10ml @19:10:21.61
delay 15 seconds @19:10:36.63 SSV.dial :spit,avoiding: :exhaust @19:10:37.98 CTV.dial CSV,avoiding: :intake @19:10:38.02
SS.reconfigure :exhale @19:10:38.10 SS.seek 0ml @19:10:38.53 SSV.dial CBV,avoiding: :exhaust @19:10:45.55 SSV.dial between CBV and plug @19:10:46.47 CBV.dial between SSV and waste2 @19:10:46.86 CSV.dial between air and CTV @19:10:47.16 CS.seek 7.399ml @19:10:53.95 CTV.dial CSV,avoiding: :air @19:10:53.98
CSV.dial CTV @19:10:54.31 CS.reconfigure :pressurize @19:10:54.36 CS.seek 0ml @19:11:10.68 BubblePoint: Intake @24.2psia, Outlet @1.3psia CBV.dial SSV @19:11:11.03 SS.reconfigure :default @19:11:11.08 SS.seek 10ml @19:11:16.63 delay 15 seconds @19:11:31.76 Evacuated: Intake @23.7psia, Outlet @12.5psia @19:11:31.77 SSV.dial :spit,avoiding: :exhaust @19:11:32.84 SS.reconfigure :exhale @19:11:32.89 SS.seek 0ml @19:11:37.77 SS.reconfigure :fast @19:11:37.82 SS.seek 5ml @19:11:40.54 SSV.dial CBV,avoiding: :exhaust @19:11:40.57
CBV.dial SSV @19:11:41.96 CTV.dial :air,avoiding: :intake @19:11:43.34 CSV.dial CTV @19:11:43.39
SS.seek 10ml @19:11:44.51 CSV.dial :air @19:11:44.97 CS.reconfigure :fast @19:11:45.02 CS.seek 0ml @19:11:45.88
SSV.dial :spit,avoiding: :exhaust @19:11:47.25 SS.reconfigure :exhale @19:11:47.30 SS.seek 0ml @19:11:48.71 CS.stop @19:11:52.16
SS.reconfigure :fast @19:11:52.20 SS.seek 5ml @19:11:54.82 SSV.dial CBV,avoiding: :exhaust @19:11:54.85
CBV.dial SSV @19:11:57.23 SSV.dial :spit,avoiding: :exhaust @19:11:58.58 SS.reconfigure :default @19:11:58.62 SS.seek 0ml @19:12:01.77 SS.stop @19:12:01.81 Lysing: Adding 2.0ml AqMeOH @19:12:01.83 CBV.dial between CSV and waste1,avoiding: :DBV @19:12:03.32 CTV.dial CSV @19:12:04.68 CSV.dial CSR @19:12:05.47 Pulling 1ml of CSR.AqMeOH @19:12:05.48 CSR.select :AqMeOH @19:12:05.97 CS.reconfigure :default @19:12:06.08 CS.seek 1ml @19:12:09.39 delay 5 seconds @19:12:14.40 CSR.select :air @19:12:14.58 CS.seek 6ml @19:12:28.23 delay 2 seconds @19:12:30.25 CSV.dial :waste2 @19:12:30.69 CS.reconfigure :fast @19:12:30.74 CS.seek 0ml @19:12:45.23 CSV.dial CSR @19:12:45.68 Pulling 2ml of CSR.AqMeOH @19:12:45.69 CSR.select :AqMeOH @19:12:45.81 CS.reconfigure :default @19:12:45.85 CS.seek 2ml @19:12:51.82 delay 5 seconds @19:12:56.84 CSR.select :air @19:12:57.00 CS.seek 5ml @19:13:05.51 delay 2 seconds @19:13:07.53 CSV.dial CTV @19:13:07.99 CS.seek 1ml @19:13:19.21 Before lysing Collection Puck Heater at 13.3C/17.7psia @19:13:19.23 Heating Puck.from 4, :sh1 in Collection Clamp to 70C for 10:00 @19:13:19.25 CH.seek 70C,8:00 @19:15:52.97 CH.hold 10:00,70C @19:15:53.16 Begun lysing Collection Puck Heater at 68C/24.7psia @19:25:53.63 Ended lysing Collection Puck Heater at 70C/19.6psia @19:25:53.65 CS.seek 0ml @19:25:57.10 CTV.dial between spare and air,avoiding: :intake @19:25:58.16 CSV.dial :air @19:25:58.60 CS.seek 5ml @19:26:12.15 CSV.dial CTV @19:26:12.61 delay 2 seconds @19:26:14.63 CSV.dial :air @19:26:15.08 delay 2 seconds @19:26:17.10 CSV.dial CTV @19:26:17.55 delay 2 seconds @19:26:19.57 CSV.dial :air @19:26:20.02 delay 2 seconds @19:26:22.04 CS.seek 0ml @19:26:35.68 Puck @27.1psia after final lysis pressurization @19:26:35.69 Recovering Lysate into Collection syringe @19:26:35.70 CSV.dial CBV @19:26:36.70 CBV.dial CSV @19:26:37.09 CS.seek 5.001ml @19:26:50.75 delay 5 seconds @19:26:55.77 CTV.dial :air @19:26:56.29 CS.seek 7.001ml @19:27:02.15 delay 5 seconds @19:27:07.17 CSV.dial :air2 @19:27:07.61 CS.seek 10ml @19:27:16.09 CSV.dial between air and CTV,avoiding: CBV @19:27:17.24 Lysing Complete @19:27:17.31 Storing sh1 puck in Collection Clamp to tube 3 @19:27:17.48 SC.reconfigure :tight @19:27:17.53 SC.seek 3 @19:27:21.20 SC.configure :limp @19:27:21.41 Elbow.reconfigure :tight @19:27:21.45 Elbow.seek 3 @19:27:23.14 Forearm.reconfigure :tight @19:27:23.19 Forearm.seek 3 + 800 counts @19:27:24.27 SE.reconfigure :pick @19:27:24.32 SE.seek :beamBreak @19:27:25.96 SE.configure :default @19:27:26.11 Elbow.reconfigure :limp @19:27:26.16 Forearm.reconfigure :loose @19:27:26.22 Forearm.seek 3 @19:27:26.97 Elbow.hold @19:27:27.03 Elbow.reconfigure :tight @19:27:27.13 SE.seek 15.1 Pucks below Top Plate @19:27:27.63 Forearm.force -20 @19:27:27.67 Forearm.seek 3 + 309 counts @19:27:28.08 Forearm.reconfigure :tight @19:27:28.13 Forearm.seek 3 + 800 counts @19:27:28.45 SE.seek 16.1 Pucks below Top Plate @19:27:29.16 Forearm.reconfigure :loose @19:27:29.20 Forearm.seek :retract @19:27:30.15 Elbow.seek CC @19:27:31.76 Elbow.reconfigure :loose @19:27:31.86 Forearm.seek CC @19:27:32.88 Hand.close @19:27:33.56 CC.seek :open @19:27:37.76 Forearm.seek :retract @19:27:38.74 Elbow.reconfigure :tight @19:27:38.79 Elbow.seek 3 @19:27:40.52 Elbow.reconfigure :limp @19:27:40.57 Forearm.seek 3 @19:27:41.69 Elbow.hold @19:27:41.77 Elbow.reconfigure :tight @19:27:41.82 Hand.open @19:27:42.67 Forearm.force -20 @19:27:42.71 Forearm.seek 3 + 320 counts @19:27:43.00 Forearm.reconfigure :tight @19:27:43.05 Forearm.seek 3 + 800 counts @19:27:43.46 SE.seek :down @19:27:44.60
Forearm.reconfigure :loose @19:27:44.65 Forearm.seek :retract @19:27:45.08 SE.stop @19:27:45.63
Tranferring DA @19:27:45.64 Beginning samplePassC @19:27:45.66 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:27:45.85 Elbow.seek FlushPuck::Garage @19:27:47.64 Elbow.reconfigure :loose @19:27:47.74 Forearm.seek :garage @19:27:48.79 Hand.close @19:27:49.67 Forearm.seek :retract @19:27:50.73 Elbow.reconfigure :tight @19:27:50.77 Elbow.seek CC @19:27:53.67 Elbow.reconfigure :loose @19:27:53.77 Forearm.reconfigure :tight @19:27:53.82 Forearm.seek CC @19:27:55.06 CC.seek :closed @19:27:59.04 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.97 @19:27:59.10 Hand.open @19:27:59.86 Forearm.reconfigure :loose @19:27:59.91 Forearm.seek :retract @19:28:00.94 PSV.dial PSR @19:28:02.32 Pulling 1.5ml of PSR.flush @19:28:02.33 PSR.select :flush @19:28:02.45 PS.seek 1.5ml @19:28:18.55 delay 5 seconds @19:28:24.01 PSV.dial :waste1 @19:28:24.48 PS.seek 0ml @19:28:40.71 PSV.dial PSR @19:28:41.17 PSR.select :air @19:28:41.30 PS.seek 2.5ml @19:29:07.71 PRV.dial CBV,avoiding: :ab2 @19:29:07.74
CBV.dial PRV @19:29:09.52 PSV.dial :mixloop @19:29:10.55 CTV.dial :air,avoiding: :intake @19:29:10.62 PTV.dial :PRVmixing @19:29:11.93 PS.seek 0ml @19:29:38.27 PSV.dial :air @19:29:38.74 PS.seek 2.5ml @19:30:05.08 PSV.dial :mixloop @19:30:05.55 PS.seek 0ml @19:30:31.92 PSV.dial :air @19:30:32.40 PS.seek 2.5ml @19:30:58.80 PSV.dial :mixloop @19:30:59.27 PS.seek 0ml @19:31:25.83 Garaging FlushPuck in Collection Clamp @19:31:25.90 Elbow.reconfigure :tight @19:31:25.95 Elbow.seek CC @19:31:26.31 Elbow.reconfigure :loose @19:31:26.41 Forearm.seek CC @19:31:27.39 Hand.close @19:31:28.18 CC.seek :open @19:31:32.27 Forearm.seek :retract @19:31:33.25 Elbow.reconfigure :tight @19:31:33.30 Elbow.seek :garage @19:31:36.12 Elbow.reconfigure :loose @19:31:36.22 Forearm.reconfigure :tight @19:31:36.27 Forearm.seek :garage @19:31:37.38 Hand.open @19:31:38.23 Forearm.reconfigure :loose @19:31:38.28 Forearm.seek :retract @19:31:39.77 PSV.dial :mixloop @19:31:39.84 PTV.dial :PRVmixing @19:31:39.93 PRV.dial CBV @19:31:39.95
CBV.dial PRV @19:31:40.15 CTV.dial :air,avoiding: :intake @19:31:40.23 Loading lyfil puck from tube 4 into Collection Clamp @19:31:40.47 SC.reconfigure :tight @19:31:40.52 SC.seek 4 @19:31:44.17 SC.configure :limp @19:31:44.38 Elbow.reconfigure :tight @19:31:44.43 Elbow.seek 4 @19:31:46.37 Forearm.reconfigure :tight @19:31:46.42 Forearm.seek 4 + 800 counts @19:31:47.47 SE.reconfigure :pick @19:31:47.53 SE.seek :beamBreak @19:31:50.16 SE.configure :default @19:31:50.32 Elbow.seek 4 @19:31:50.57 Forearm.seek 4 + 300 counts @19:31:50.74 Forearm.reconfigure :loose @19:31:50.78 Hand.close @19:31:51.56 SE.seek :down @19:31:51.76
Forearm.seek :retract @19:31:52.71 Elbow.seek CC @19:31:53.96 SE.stop @19:31:54.34
Elbow.reconfigure :loose @19:31:54.44 Forearm.reconfigure :tight @19:31:54.49 Forearm.seek CC @19:31:56.23 CC.seek :closed @19:32:00.25 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.98 @19:32:00.26 Hand.open @19:32:01.11 Forearm.reconfigure :loose @19:32:01.16 Forearm.seek :retract @19:32:02.14 CTV.dial CSV,avoiding: :intake @19:32:03.51 CSV.dial CTV @19:32:03.83 PS.seek 2.5ml @19:32:30.20 CS.seek 5ml @19:32:43.81 delay 8 seconds @19:32:51.82 PSV.dial :air @19:32:52.28 delay 2 seconds @19:32:54.30 PSV.dial :waste2 @19:32:55.04 PS.seek 0ml @19:33:21.42 CBV.dial between PRV and waste1 @19:33:21.74 CSV.dial :air @19:33:22.20 CS.seek 10ml @19:33:35.87 CSV.dial CTV @19:33:36.33 CS.seek 0ml @19:34:02.74 CBV.dial :waste1 @19:34:03.07 delay 5 seconds @19:34:08.09 CTV.dial :air,avoiding: :intake @19:34:09.51 Storing lyfil puck in Collection Clamp to tube 3 @19:34:09.68 SC.reconfigure :tight @19:34:09.73 SC.seek 3 @19:34:13.42 SC.configure :limp @19:34:13.63 Elbow.reconfigure :tight @19:34:13.68 Elbow.seek 3 @19:34:15.75 Forearm.reconfigure :tight @19:34:15.87 Forearm.seek 3 + 800 counts @19:34:17.01 SE.reconfigure :pick @19:34:17.07 SE.seek :beamBreak @19:34:18.66 SE.configure :default @19:34:18.83 Elbow.reconfigure :limp @19:34:18.88 Forearm.reconfigure :loose @19:34:18.93 Forearm.seek 3 @19:34:19.31 Elbow.hold @19:34:20.06 Elbow.reconfigure :tight @19:34:20.16 SE.seek 16.1 Pucks below Top Plate @19:34:20.64 Forearm.force -20 @19:34:20.68 Forearm.seek 3 + 289 counts @19:34:21.04 Forearm.reconfigure :tight @19:34:21.09 Forearm.seek 3 + 800 counts @19:34:21.50 SE.seek 17.1 Pucks below Top Plate @19:34:22.12 Forearm.reconfigure :loose @19:34:22.16 Forearm.seek :retract @19:34:23.11 Elbow.seek CC @19:34:24.73 Elbow.reconfigure :loose @19:34:24.83 Forearm.seek CC @19:34:25.78 Hand.close @19:34:26.55 CC.seek :open @19:34:30.76 Forearm.seek :retract @19:34:31.70 Elbow.reconfigure :tight @19:34:31.75 Elbow.seek 3 @19:34:33.47 Elbow.reconfigure :limp @19:34:33.90 Forearm.seek 3 @19:34:35.11 Elbow.hold @19:34:35.18 Elbow.reconfigure :tight @19:34:35.22 Hand.open @19:34:36.04 Forearm.force -20 @19:34:36.09 Forearm.seek 3 + 319 counts @19:34:36.37 Forearm.reconfigure :tight @19:34:36.42 Forearm.seek 3 + 800 counts @19:34:36.78 SE.seek :down @19:34:37.85 SE.stop @19:34:37.99
Forearm.reconfigure :loose @19:34:38.04 Forearm.seek :retract @19:34:38.94 PSV.dial :air @19:34:39.69 PS.seek 2.5ml @19:35:06.04 delay 1 second @19:35:07.06 PSV.dial :mixloop @19:35:07.62 delay 1 second @19:35:08.64 PSV.dial :air @19:35:09.09 delay 1 second @19:35:10.11 PSV.dial :mixloop @19:35:10.58 delay 1 second @19:35:11.59 PSV.dial :air @19:35:12.06 delay 1 second @19:35:13.08 PS.seek 0ml @19:35:39.42 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @19:35:39.60 Elbow.seek FlushPuck::Garage @19:35:41.37 Elbow.reconfigure :loose @19:35:41.47 Forearm.seek :garage @19:35:42.51 Hand.close @19:35:43.41 Forearm.seek :retract @19:35:44.43 Elbow.reconfigure :tight @19:35:44.48 Elbow.seek PC @19:35:45.80 Elbow.reconfigure :loose @19:35:46.28 Forearm.reconfigure :tight @19:35:46.39 Forearm.seek PC @19:35:47.78 PC.seek :closed @19:35:52.21 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.55 @19:35:52.27 Hand.open @19:35:53.11 Forearm.reconfigure :loose @19:35:53.16 Forearm.seek :retract @19:35:54.16 PRV.dial :air @19:35:54.63 PSV.dial :mixloop @19:35:55.15 PS.seek 0.3ml @19:35:59.04 PSV.dial PSR @19:36:00.09 Pulling 0.4ml of PSR.air @19:36:00.10 PSR.select :air @19:36:00.12 PS.seek 0.7ml @19:36:05.04 delay 3 seconds @19:36:08.06 PSR.select :air @19:36:08.08 PS.seek 2.5ml @19:36:27.32 delay 3 seconds @19:36:30.34 PSV.dial :mixloop @19:36:31.39 PTV.dial :mixing @19:36:32.12 PBV.dial :waste2 @19:36:33.13 PS.seek 0ml @19:36:59.67 Garaging FlushPuck in Processing Clamp @19:36:59.74 Elbow.reconfigure :tight @19:36:59.79 Elbow.seek PC @19:36:59.96 Elbow.reconfigure :loose @19:37:00.06 Forearm.seek PC @19:37:01.15 Hand.close @19:37:01.92 PC.seek :open @19:37:06.41 Forearm.seek :retract @19:37:07.82 Elbow.reconfigure :tight @19:37:07.93 Elbow.seek :garage @19:37:09.32 Elbow.reconfigure :loose @19:37:09.42 Forearm.reconfigure :tight @19:37:09.47 Forearm.seek :garage @19:37:10.59 Hand.open @19:37:11.36 Forearm.reconfigure :loose @19:37:11.41 Forearm.seek :retract @19:37:12.39 Loading da puck from tube 4 into Processing Clamp @19:37:12.63 SC.reconfigure :tight @19:37:12.68 SC.seek 4 @19:37:16.34 SC.configure :limp @19:37:16.56 Elbow.reconfigure :tight @19:37:16.61 Elbow.seek 4 @19:37:18.59 Forearm.reconfigure :tight @19:37:18.63 Forearm.seek 4 + 800 counts @19:37:19.70 SE.reconfigure :pick @19:37:19.76 SE.seek :beamBreak @19:37:22.48 SE.configure :default @19:37:22.64 Elbow.seek 4 @19:37:22.88 Forearm.seek 4 + 300 counts @19:37:23.04 Forearm.reconfigure :loose @19:37:23.09 Hand.close @19:37:23.80 SE.seek :down @19:37:24.00
Forearm.seek :retract @19:37:24.98 Elbow.seek PC @19:37:26.33 SE.stop @19:37:27.52
Elbow.reconfigure :loose @19:37:27.62 Forearm.reconfigure :tight @19:37:27.67 Forearm.seek PC @19:37:29.50 PC.seek :closed @19:37:33.92 Processing Clamp onSpring/offSpring for 11/26 samples, on/off torque 1.52 @19:37:33.93 Hand.open @19:37:34.73 Forearm.reconfigure :loose @19:37:34.77 Forearm.seek :retract @19:37:35.93 Heating Puck.from 4, :da in Processing Clamp to 28C for 2:00:00 @19:37:35.94 PH.seek 28C,5:00 @19:38:24.96 PH.hold 2:00:00,28C @19:38:25.01 delay 1:30 @19:39:55.06 PSR.select :air @19:39:55.08 PSV.dial PSR @19:39:56.14 Pulling 0.5ml of PSR.wash @19:39:56.15 PSR.select :wash @19:39:56.27 PS.seek 0.5ml @19:40:02.14 delay 5 seconds @19:40:07.16 PSR.select :air @19:40:07.28 PS.seek 2ml @19:40:23.39 PTV.dial :puck @19:40:24.14 PSV.dial PTV @19:40:24.90 PBV.dial :waste1 @19:40:26.34 PS.seek 1.5ml @19:40:32.26 delay 3 seconds @19:40:35.28 PBV.dial between PSV and waste1 @19:40:35.66 PS.seek 0ml @19:40:51.76 delay 5:00 @19:45:51.79 recoverPSRreagent08 @19:45:51.80 PTV.dial :puck @19:45:51.87 PBV.dial :waste1 @19:45:52.26 delay 30 seconds @19:46:22.28 PBV.dial between PSV and waste1 @19:46:22.68 PSR.select :air @19:46:22.70 PSV.dial PSR @19:46:23.45 PS.reconfigure :fast @19:46:23.50 PS.seek 2.5ml @19:46:46.85 PSV.dial PTV @19:46:47.60 delay 5 seconds @19:46:52.62 PSV.dial PSR @19:46:53.38 delay 5 seconds @19:46:58.40 PSV.dial PTV @19:46:59.15 delay 5 seconds @19:47:04.17 PSV.dial :air @19:47:04.93 PS.seek 0ml @19:47:28.07 PTV.dial :PRVmixing,via: PRV @19:47:29.38 PSV.dial :mixloop @19:47:29.85 PRV.dial :ab1 @19:47:30.61 PS.reconfigure :slow @19:47:30.65 PS.seek 0.15ml @19:47:34.19 delay 2 seconds @19:47:36.21 CBV.dial PRV @19:47:36.25
PRV.dial CBV @19:47:37.27 PS.seek 0.34ml @19:47:41.63 delay 2 seconds @19:47:43.65 CBV.dial between PRV and PSV @19:47:43.98 PRV.dial :air @19:47:44.43 PS.seek 0.9ml @19:47:56.44 PRV.dial :waste2 @19:47:57.20 PS.seek 0.5ml @19:48:05.85 addPMLReagent08 @19:48:05.86 PSV.dial :air @19:48:06.32 PS.reconfigure :default @19:48:06.37 PS.seek 2ml @19:48:22.47 PSV.dial :PML @19:48:22.94 PTV.dial :mixing @19:48:23.68 PBV.dial :waste1 @19:48:24.13 PS.seek 1.85ml @19:48:26.50 PBV.dial between PSV and waste1 @19:48:26.89 PS.seek 0ml @19:48:46.57 delay 2 seconds @19:48:48.59 Beginning samplePassC @19:48:48.60 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:48:48.79 Elbow.reconfigure :tight @19:48:48.83 Elbow.seek FlushPuck::Garage @19:48:50.15 Elbow.reconfigure :loose @19:48:50.25 Forearm.seek :garage @19:48:51.27 Hand.close @19:48:52.15 Forearm.seek :retract @19:48:53.16 Elbow.reconfigure :tight @19:48:53.21 Elbow.seek CC @19:48:56.00 Elbow.reconfigure :loose @19:48:56.10 Forearm.reconfigure :tight @19:48:56.14 Forearm.seek CC @19:48:57.47 CC.seek :closed @19:49:01.47 Collection Clamp onSpring/offSpring for 7/27 samples, on/off torque 1.94 @19:49:01.54 Hand.open @19:49:02.36 Forearm.reconfigure :loose @19:49:02.40 Forearm.seek :retract @19:49:03.44 PSV.dial PSR @19:49:04.49 Pulling 1.5ml of PSR.flush @19:49:04.50 PSR.select :flush @19:49:04.63 PS.seek 1.5ml @19:49:20.82 delay 5 seconds @19:49:25.84 PSV.dial :waste1 @19:49:26.29 PS.seek 0ml @19:49:42.44 PSV.dial PSR @19:49:42.91 PSR.select :air @19:49:43.03 PS.seek 2.5ml @19:50:09.38 PRV.dial CBV,avoiding: :ab2 @19:50:09.41
CBV.dial PRV @19:50:11.05 PSV.dial :mixloop @19:50:12.10 CTV.dial :air,avoiding: :intake @19:50:12.17 PTV.dial :PRVmixing @19:50:12.93 PS.seek 0ml @19:50:39.25 PSV.dial :air @19:50:39.84 PS.seek 2.5ml @19:51:06.16 PSV.dial :mixloop @19:51:06.62 PS.seek 0ml @19:51:32.94 PSV.dial :air @19:51:33.42 PS.seek 2.5ml @19:51:59.82 PSV.dial :mixloop @19:52:00.29 PS.seek 0ml @19:52:26.60 Garaging FlushPuck in Collection Clamp @19:52:26.67 Elbow.reconfigure :tight @19:52:26.71 Elbow.seek CC @19:52:26.84 Elbow.reconfigure :loose @19:52:26.94 Forearm.seek CC @19:52:27.97 Hand.close @19:52:28.66 CC.seek :open @19:52:33.21 Forearm.seek :retract @19:52:34.20 Elbow.reconfigure :tight @19:52:34.25 Elbow.seek :garage @19:52:37.12 Elbow.reconfigure :loose @19:52:37.22 Forearm.reconfigure :tight @19:52:37.27 Forearm.seek :garage @19:52:38.34 Hand.open @19:52:39.09 Forearm.reconfigure :loose @19:52:39.14 Forearm.seek :retract @19:52:40.13 delayUntil "19:56:48.58" @19:52:40.15 Whole Cell Archive sampling 1000ml @19:52:40.17 Loading wcr puck from tube 4 into Collection Clamp @19:52:40.41 SC.reconfigure :tight @19:52:40.46 SC.seek 4 @19:52:40.53 SC.configure :limp @19:52:40.75 Elbow.reconfigure :tight @19:52:40.80 Elbow.seek 4 @19:52:42.70 Forearm.reconfigure :tight @19:52:42.75 Forearm.seek 4 + 800 counts @19:52:43.74 SE.reconfigure :pick @19:52:43.80 SE.seek :beamBreak @19:52:46.61 SE.configure :default @19:52:46.77 Elbow.seek 4 @19:52:46.97 Forearm.seek 4 + 300 counts @19:52:47.20 Forearm.reconfigure :loose @19:52:47.25 Hand.close @19:52:48.04 SE.seek :down @19:52:48.69 Forearm.seek :retract @19:52:49.67 Elbow.seek CC @19:52:50.66 SE.stop @19:52:51.27 Elbow.reconfigure :loose @19:52:51.38 Forearm.reconfigure :tight @19:52:51.43 Forearm.seek CC @19:52:52.73 CC.seek :closed @19:52:56.71 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 1.96 @19:52:56.72 Hand.open @19:52:57.47 Forearm.reconfigure :loose @19:52:57.52 Forearm.seek :retract @19:52:58.52 collectSample 1000ml w/bubble point of 35psi @19:52:58.53 Priming 7ml @19:52:58.54 Intake.select :close @19:52:58.55 Exhaust.select :close @19:52:58.56 SS.hold @19:52:58.79 Calibrating pressure sensors @19:52:58.80 CTV.dial :air @19:52:58.81 SSV.dial :spit,avoiding: :exhaust @19:52:58.89 SS.reconfigure :fast @19:52:58.94 SS.seek 5ml @19:53:01.68 SSV.dial CBV @19:53:01.71 CBV.dial SSV @19:53:03.09 delay 3.8 seconds @19:53:07.02 Sensor limits: Intake max 95.6psia; Outlet max 106.8psia @19:53:07.03 SSV.dial :spit @19:53:08.37 SS.seek 0ml @19:53:11.16 SSV.dial CBV @19:53:12.53 CTV.dial :intake @19:53:13.31 SSV.dial CBV @19:53:13.34 CBV.dial SSV @19:53:13.93 Allowing 40s for 7.0ml of remaining 7.0ml @19:53:13.94 Intake.select :open @19:53:16.11 Intake @14.2psia, vacuum from 8.7 to 11.7psi @19:53:16.13 SS.reconfigure :inhaling 2.17ml/s @19:53:16.17 SS.seek 7ml @19:53:22.59 Stroke completed -- waiting for pressure to equalize ... @19:53:22.60 SS.reconfigure :exhale @19:53:22.65 SS.hold @19:53:29.05 Intake.select :close @19:53:29.21 SSV.dial :exhaust @19:53:29.99 Exhaust.select :open @19:53:30.11 SS.seek 0ml @19:53:34.15 Exhaust.select :close @19:53:34.26 Sampling 1000.0ml @19:53:34.27 Intake.select :close @19:53:34.28 Exhaust.select :close @19:53:34.30 SS.reconfigure :default @19:53:34.34 SS.hold @19:53:34.56 CTV.dial :intake @19:53:34.73 SSV.dial CBV @19:53:34.77 CBV.dial SSV @19:53:35.54 Allowing 130s for 25.0ml of remaining 1000.0ml @19:53:35.55 Intake.select :open @19:53:37.72 Intake @13.7psia, vacuum from 8.2 to 11.2psi @19:53:37.73 SS.reconfigure :inhaling 2.17ml/s @19:53:38.24 SS.seek 25ml @19:53:48.67 Vacuum too high -- waiting up to 40 seconds @19:53:48.69 SS.reconfigure :exhale @19:53:48.74 SS.hold @19:53:49.73 Intake @9.8psia, vacuum from 4.3 to 7.3psi @19:53:49.75 SS.reconfigure :inhaling 1.95ml/s @19:53:49.80 SS.seek 25ml @19:53:49.87 Vacuum too high -- waiting up to 40 seconds @19:53:49.89 SS.reconfigure :exhale @19:53:49.94 SS.hold @19:54:01.18 Intake @12.5psia, vacuum from 7.0 to 10.0psi @19:54:01.20 SS.reconfigure :inhaling 1.77ml/s @19:54:01.25 SS.seek 25ml @19:54:05.89 Vacuum too high -- waiting up to 40 seconds @19:54:05.91 SS.reconfigure :exhale @19:54:05.96 SS.hold @19:54:07.28 Intake @10.5psia, vacuum from 5.0 to 8.0psi @19:54:07.30 SS.reconfigure :inhaling 1.59ml/s @19:54:07.35 SS.seek 25ml @19:54:10.05 Stroke completed -- waiting for pressure to equalize ... @19:54:10.06 SS.reconfigure :exhale @19:54:10.11 SS.hold @19:54:15.76 Intake.select :close @19:54:15.92 SSV.dial :exhaust @19:54:16.69 Exhaust.select :open @19:54:16.81 SS.seek 0ml @19:54:26.19 Exhaust.select :close @19:54:26.31 SSV.dial CBV @19:54:26.33 CBV.dial SSV @19:54:27.13 Allowing 130s for 25.0ml of remaining 975.0ml @19:54:27.14 Intake.select :open @19:54:29.31 Intake @12.9psia, vacuum from 7.4 to 10.4psi @19:54:29.33 SS.reconfigure :inhaling 1.59ml/s @19:54:29.37 SS.seek 25ml @19:54:34.89 Vacuum too high -- waiting up to 40 seconds @19:54:34.90 SS.reconfigure :exhale @19:54:34.96 SS.hold @19:54:36.22 Intake @9.6psia, vacuum from 4.1 to 7.1psi @19:54:36.24 SS.reconfigure :inhaling 1.43ml/s @19:54:36.29 SS.seek 25ml @19:54:38.89 Vacuum too high -- waiting up to 40 seconds @19:54:38.91 SS.reconfigure :exhale @19:54:38.96 SS.hold @19:54:42.71 Intake @12.3psia, vacuum from 6.8 to 9.8psi @19:54:42.73 SS.reconfigure :inhaling 1.29ml/s @19:54:42.78 SS.seek 25ml @19:54:47.95 Vacuum too high -- waiting up to 40 seconds @19:54:47.97 SS.reconfigure :exhale @19:54:48.02 SS.hold @19:54:48.77 Intake @8.3psia, vacuum from 2.8 to 5.8psi @19:54:48.79 SS.reconfigure :inhaling 1.16ml/s @19:54:48.84 SS.seek 25ml @19:54:51.83 Vacuum too high -- waiting up to 40 seconds @19:54:51.84 SS.reconfigure :exhale @19:54:51.90 SS.hold @19:54:56.01 Intake @11.8psia, vacuum from 6.3 to 9.3psi @19:54:56.03 SS.reconfigure :inhaling 1.04ml/s @19:54:56.08 SS.seek 25ml @19:55:02.83 Vacuum too high -- waiting up to 40 seconds @19:55:02.84 SS.reconfigure :exhale @19:55:02.90 SS.hold @19:55:04.61 Intake @11.1psia, vacuum from 5.6 to 8.6psi @19:55:04.63 SS.reconfigure :inhaling 0.94ml/s @19:55:04.68 SS.seek 25ml @19:55:06.91 Stroke completed -- waiting for pressure to equalize ... @19:55:06.92 SS.reconfigure :exhale @19:55:06.97 SS.hold @19:55:10.53 Intake.select :close @19:55:10.69 SSV.dial :exhaust @19:55:11.46 Exhaust.select :open @19:55:11.57 SS.seek 0ml @19:55:20.83 Exhaust.select :close @19:55:20.95 SSV.dial CBV @19:55:20.97 CBV.dial SSV @19:55:22.24 Allowing 130s for 25.0ml of remaining 950.0ml @19:55:22.25 Intake.select :open @19:55:24.43 Intake @13.4psia, vacuum from 7.9 to 10.9psi @19:55:24.44 SS.reconfigure :inhaling 0.94ml/s @19:55:24.49 SS.seek 25ml @19:55:36.95 Vacuum too high -- waiting up to 40 seconds @19:55:36.97 SS.reconfigure :exhale @19:55:37.02 SS.hold @19:55:37.46 Intake @10.8psia, vacuum from 5.3 to 8.3psi @19:55:37.48 SS.reconfigure :inhaling 0.85ml/s @19:55:37.53 SS.seek 25ml @19:55:42.30 Vacuum too high -- waiting up to 40 seconds @19:55:42.32 SS.reconfigure :exhale @19:55:42.37 SS.hold @19:55:45.46 Intake @11.6psia, vacuum from 6.1 to 9.1psi @19:55:45.48 SS.reconfigure :inhaling 0.77ml/s @19:55:45.53 SS.seek 25ml @19:56:00.47 Stroke completed -- waiting for pressure to equalize ... @19:56:00.48 SS.reconfigure :exhale @19:56:00.53 SS.hold @19:56:05.90 Intake.select :close @19:56:06.06 SSV.dial :exhaust @19:56:06.82 Exhaust.select :open @19:56:06.94 SS.seek 0ml @19:56:16.21 Exhaust.select :close @19:56:16.32 SSV.dial CBV @19:56:16.36 CBV.dial SSV @19:56:17.13 Allowing 130s for 25.0ml of remaining 925.0ml @19:56:17.14 Intake.select :open @19:56:19.31 Intake @13.2psia, vacuum from 7.7 to 10.7psi @19:56:19.32 SS.reconfigure :inhaling 0.77ml/s @19:56:19.37 SS.seek 25ml @19:56:39.92 Vacuum too high -- waiting up to 40 seconds @19:56:39.93 SS.reconfigure :exhale @19:56:39.99 SS.hold @19:56:41.33 Intake @11.7psia, vacuum from 6.2 to 9.2psi @19:56:41.35 SS.reconfigure :inhaling 0.70ml/s @19:56:41.40 SS.seek 25ml @19:56:48.59 recoverPRVreagent08 @19:56:48.60 PTV.dial :mixing @19:56:49.34 PBV.dial :waste1 @19:56:49.73 delay 30 seconds @19:56:56.21 Stroke completed -- waiting for pressure to equalize ... @19:56:56.22 SS.reconfigure :exhale @19:56:56.27 SS.hold @19:57:01.26 Intake.select :close @19:57:01.42 SSV.dial :exhaust @19:57:02.19 Exhaust.select :open @19:57:02.30 SS.seek 0ml @19:57:11.55 Exhaust.select :close @19:57:11.66 SSV.dial CBV @19:57:11.69 CBV.dial SSV @19:57:12.49 Allowing 130s for 25.0ml of remaining 900.0ml @19:57:12.50 Intake.select :open @19:57:14.66 Intake @13.6psia, vacuum from 8.1 to 11.1psi @19:57:14.68 SS.reconfigure :inhaling 0.70ml/s @19:57:14.73 SS.seek 25ml @19:57:19.75 PBV.dial between PSV and waste1 @19:57:20.14 PSV.dial :air @19:57:20.61 PS.reconfigure :fast @19:57:20.66 PS.seek 2.5ml @19:57:44.15 PSV.dial :mixloop @19:57:44.62 delay 2 seconds @19:57:46.64 PSV.dial :air @19:57:47.11 delay 2 seconds @19:57:49.13 PSV.dial :mixloop @19:57:49.59 delay 2 seconds @19:57:51.61 PSV.dial :air @19:57:51.92 Stroke completed -- waiting for pressure to equalize ... @19:57:51.94 SS.reconfigure :exhale @19:57:51.98 SS.hold @19:57:52.08 PS.seek 0ml @19:57:58.53 Intake.select :close @19:57:58.69 SSV.dial :exhaust @19:57:59.47 Exhaust.select :open @19:57:59.58 SS.seek 0ml @19:58:08.89 Exhaust.select :close @19:58:09.00 SSV.dial CBV @19:58:09.04 CBV.dial SSV @19:58:09.82 Allowing 130s for 25.0ml of remaining 875.0ml @19:58:09.83 Intake.select :open @19:58:12.00 Intake @13.4psia, vacuum from 7.9 to 10.9psi @19:58:12.02 SS.reconfigure :inhaling 0.70ml/s @19:58:12.07 SS.seek 25ml @19:58:15.62 washCycleDA #1 of 2 @19:58:15.63 PSR.select :air @19:58:15.65 PSV.dial PSR @19:58:17.00 Pulling 0.5ml of PSR.wash @19:58:17.01 PSR.select :wash @19:58:17.13 PS.reconfigure :default @19:58:17.18 PS.seek 0.5ml @19:58:23.19 delay 5 seconds @19:58:28.21 PSR.select :air @19:58:28.33 PS.seek 2ml @19:58:40.10 Vacuum too high -- waiting up to 40 seconds @19:58:40.12 SS.reconfigure :exhale @19:58:40.17 SS.hold @19:58:40.60 Intake @10.5psia, vacuum from 5.0 to 8.0psi @19:58:40.62 SS.reconfigure :inhaling 0.62ml/s @19:58:40.67 SS.seek 25ml @19:58:44.44 PTV.dial :puck @19:58:45.21 PSV.dial PTV @19:58:45.95 PBV.dial :waste1 @19:58:46.40 PS.seek 1.5ml @19:58:51.02 Stroke completed -- waiting for pressure to equalize ... @19:58:51.03 SS.reconfigure :exhale @19:58:51.08 SS.hold @19:58:52.46 delay 3 seconds @19:58:55.48 PBV.dial between PSV and waste1 @19:58:55.88 PS.seek 0ml @19:58:57.24 Intake.select :close @19:58:57.40 SSV.dial :exhaust @19:58:58.17 Exhaust.select :open @19:58:58.29 SS.seek 0ml @19:59:07.53 Exhaust.select :close @19:59:07.64 SSV.dial CBV @19:59:07.68 CBV.dial SSV @19:59:08.49 Allowing 130s for 25.0ml of remaining 850.0ml @19:59:08.50 Intake.select :open @19:59:10.67 Intake @13.7psia, vacuum from 8.2 to 11.2psi @19:59:10.69 SS.reconfigure :inhaling 0.62ml/s @19:59:10.73 SS.seek 25ml @19:59:12.19 delay 2:00 @19:59:52.26 Stroke completed -- waiting for pressure to equalize ... @19:59:52.28 SS.reconfigure :exhale @19:59:52.33 SS.hold @19:59:58.07 Intake.select :close @19:59:58.23 SSV.dial :exhaust @19:59:58.98 Exhaust.select :open @19:59:59.10 SS.seek 0ml @20:00:08.44 Exhaust.select :close @20:00:08.55 SSV.dial CBV @20:00:08.59 CBV.dial SSV @20:00:09.36 Allowing 130s for 25.0ml of remaining 825.0ml @20:00:09.37 Intake.select :open @20:00:11.54 Intake @13.6psia, vacuum from 8.1 to 11.1psi @20:00:11.55 SS.reconfigure :inhaling 0.62ml/s @20:00:11.60 SS.seek 25ml @20:00:52.83 Stroke completed -- waiting for pressure to equalize ... @20:00:52.84 SS.reconfigure :exhale @20:00:52.89 SS.hold @20:00:59.43 Intake.select :close @20:01:00.04 SSV.dial :exhaust @20:01:00.81 Exhaust.select :open @20:01:00.93 SS.seek 0ml @20:01:10.25 Exhaust.select :close @20:01:10.36 SSV.dial CBV @20:01:10.39 CBV.dial SSV @20:01:11.22 Allowing 130s for 25.0ml of remaining 800.0ml @20:01:11.23 Intake.select :open @20:01:12.21 recoverPSRreagent08 @20:01:12.22 PTV.dial :puck @20:01:12.29 PBV.dial :waste1 @20:01:12.68 delay 30 seconds @20:01:13.40 Intake @14.0psia, vacuum from 8.5 to 11.5psi @20:01:13.42 SS.reconfigure :inhaling 0.62ml/s @20:01:13.46 SS.seek 25ml @20:01:42.71 PBV.dial between PSV and waste1 @20:01:43.11 PSR.select :air @20:01:43.13 PSV.dial PSR @20:01:43.88 PS.reconfigure :fast @20:01:43.93 PS.seek 2.5ml @20:01:54.68 Stroke completed -- waiting for pressure to equalize ... @20:01:54.70 SS.reconfigure :exhale @20:01:54.75 SS.hold @20:02:02.23 Intake.select :close @20:02:02.78 SSV.dial :exhaust @20:02:03.62 Exhaust.select :open @20:02:03.74 SS.seek 0ml @20:02:07.43 PSV.dial PTV @20:02:08.20 delay 5 seconds @20:02:13.04 Exhaust.select :close @20:02:13.16 SSV.dial CBV @20:02:13.18 CBV.dial SSV @20:02:13.28 PSV.dial PSR @20:02:13.98 Allowing 130s for 25.0ml of remaining 775.0ml @20:02:14.00 Intake.select :open @20:02:14.08 delay 5 seconds @20:02:16.17 Intake @13.8psia, vacuum from 8.3 to 11.3psi @20:02:16.19 SS.reconfigure :inhaling 0.62ml/s @20:02:16.23 SS.seek 25ml @20:02:19.09 PSV.dial PTV @20:02:19.84 delay 5 seconds @20:02:24.88 PSV.dial :air @20:02:25.64 PS.seek 0ml @20:02:42.91 Vacuum too high -- waiting up to 40 seconds @20:02:42.93 SS.reconfigure :exhale @20:02:42.98 SS.hold @20:02:45.11 Intake @12.1psia, vacuum from 6.6 to 9.6psi @20:02:45.13 SS.reconfigure :inhaling 0.56ml/s @20:02:45.18 SS.seek 25ml @20:02:48.97 washCycleDA #2 of 2 @20:02:48.98 PSR.select :air @20:02:48.99 PSV.dial PSR @20:02:50.35 Pulling 0.5ml of PSR.wash @20:02:50.36 PSR.select :wash @20:02:50.48 PS.reconfigure :default @20:02:50.53 PS.seek 0.5ml @20:02:56.43 delay 5 seconds @20:03:01.45 PSR.select :air @20:03:01.57 PS.seek 2ml @20:03:01.64 Stroke completed -- waiting for pressure to equalize ... @20:03:01.65 SS.reconfigure :exhale @20:03:01.70 SS.hold @20:03:09.55 Intake.select :close @20:03:09.72 SSV.dial :exhaust @20:03:10.48 Exhaust.select :open @20:03:10.59 SS.seek 0ml @20:03:17.74 PTV.dial :puck @20:03:17.82 PSV.dial PTV @20:03:18.59 PBV.dial :waste1 @20:03:19.04 PS.seek 1.5ml @20:03:19.89 Exhaust.select :close @20:03:20.00 SSV.dial CBV @20:03:20.04 CBV.dial SSV @20:03:20.83 Allowing 130s for 25.0ml of remaining 750.0ml @20:03:20.84 Intake.select :open @20:03:23.01 Intake @14.4psia, vacuum from 8.9 to 11.9psi @20:03:23.03 SS.reconfigure :inhaling 0.56ml/s @20:03:23.07 SS.seek 25ml @20:03:24.93 delay 3 seconds @20:03:27.95 PBV.dial between PSV and waste1 @20:03:28.35 PS.seek 0ml @20:03:44.57 delay 2:00 @20:04:08.63 Stroke completed -- waiting for pressure to equalize ... @20:04:08.65 SS.reconfigure :exhale @20:04:08.69 SS.hold @20:04:18.76 Intake.select :close @20:04:18.92 SSV.dial :exhaust @20:04:19.70 Exhaust.select :open @20:04:19.82 SS.seek 0ml @20:04:29.15 Exhaust.select :close @20:04:29.26 SSV.dial CBV @20:04:29.29 CBV.dial SSV @20:04:30.12 Allowing 130s for 25.0ml of remaining 725.0ml @20:04:30.13 Intake.select :open @20:04:32.30 Intake @14.5psia, vacuum from 9.0 to 12.0psi @20:04:32.32 SS.reconfigure :inhaling 0.56ml/s @20:04:32.37 SS.seek 25ml @20:04:51.41 Vacuum too high -- waiting up to 40 seconds @20:04:51.42 SS.reconfigure :exhale @20:04:51.48 SS.hold @20:04:54.24 Intake @13.2psia, vacuum from 7.7 to 10.7psi @20:04:54.26 SS.reconfigure :inhaling 0.51ml/s @20:04:54.30 SS.seek 25ml @20:05:04.82 Vacuum too high -- waiting up to 40 seconds @20:05:04.83 SS.reconfigure :exhale @20:05:04.89 SS.hold @20:05:09.15 Intake @13.3psia, vacuum from 7.8 to 10.8psi @20:05:09.17 SS.reconfigure :inhaling 0.46ml/s @20:05:09.22 SS.seek 25ml @20:05:30.13 Stroke completed -- waiting for pressure to equalize ... @20:05:30.14 SS.reconfigure :exhale @20:05:30.19 SS.hold @20:05:41.35 Intake.select :close @20:05:41.51 SSV.dial :exhaust @20:05:42.27 Exhaust.select :open @20:05:42.39 SS.seek 0ml @20:05:44.59 recoverPSRreagent08 @20:05:44.60 PTV.dial :puck @20:05:44.68 PBV.dial :waste1 @20:05:45.07 delay 30 seconds @20:05:51.73 Exhaust.select :close @20:05:51.84 SSV.dial CBV @20:05:51.88 CBV.dial SSV @20:05:52.65 Allowing 130s for 25.0ml of remaining 700.0ml @20:05:52.66 Intake.select :open @20:05:54.83 Intake @13.8psia, vacuum from 8.3 to 11.3psi @20:05:54.84 SS.reconfigure :inhaling 0.46ml/s @20:05:54.89 SS.seek 25ml @20:06:15.09 PBV.dial between PSV and waste1 @20:06:15.49 PSR.select :air @20:06:15.51 PSV.dial PSR @20:06:16.26 PS.reconfigure :fast @20:06:16.31 PS.seek 2.5ml @20:06:38.16 Vacuum too high -- waiting up to 40 seconds @20:06:38.17 SS.reconfigure :exhale @20:06:38.23 SS.hold @20:06:39.88 PSV.dial PTV @20:06:40.78 delay 5 seconds @20:06:43.39 Intake @13.6psia, vacuum from 8.1 to 11.1psi @20:06:43.41 SS.reconfigure :inhaling 0.42ml/s @20:06:43.46 SS.seek 25ml @20:06:45.80 PSV.dial PSR @20:06:46.57 delay 5 seconds @20:06:51.59 PSV.dial PTV @20:06:52.35 delay 5 seconds @20:06:56.58 Stroke completed -- waiting for pressure to equalize ... @20:06:56.60 SS.reconfigure :exhale @20:06:56.65 SS.hold @20:06:57.37 PSV.dial :air @20:06:58.14 PS.seek 0ml @20:07:07.91 Intake.select :close @20:07:08.08 SSV.dial :exhaust @20:07:08.84 Exhaust.select :open @20:07:08.96 SS.seek 0ml @20:07:18.23 Exhaust.select :close @20:07:18.34 SSV.dial CBV @20:07:18.38 CBV.dial SSV @20:07:19.15 Allowing 130s for 25.0ml of remaining 675.0ml @20:07:19.16 Intake.select :open @20:07:21.33 Intake @14.7psia, vacuum from 9.2 to 12.2psi @20:07:21.35 SS.reconfigure :inhaling 0.42ml/s @20:07:21.39 SS.seek 25ml @20:07:21.68 getPMLReagent08 @20:07:21.69 PRV.dial :air @20:07:22.15 delay 5 seconds @20:07:27.17 PTV.dial :PRVmixing @20:07:28.49 delay 5 seconds @20:07:33.51 PRV.dial :ab2 @20:07:33.98 PTV.dial :PRVmixing @20:07:33.99 PSV.dial :mixloop @20:07:34.47 PS.reconfigure :default @20:07:34.52 PS.seek 0.3ml @20:07:38.46 delay 2 seconds @20:07:40.48 PRV.dial :air @20:07:41.02 PS.seek 0.55ml @20:07:44.40 addPMLReagent08 @20:07:44.41 PSV.dial :air @20:07:44.89 PS.seek 2ml @20:08:00.55 PSV.dial :PML @20:08:01.02 PTV.dial :mixing @20:08:01.77 PBV.dial :waste1 @20:08:02.22 PS.seek 1.85ml @20:08:04.55 PBV.dial between PSV and waste1 @20:08:04.95 PS.seek 0ml @20:08:22.29 Stroke completed -- waiting for pressure to equalize ... @20:08:22.30 SS.reconfigure :exhale @20:08:22.35 SS.hold @20:08:24.65 delay 2 seconds @20:08:26.66 delay 8:00 @20:08:36.83 Intake.select :close @20:08:36.99 SSV.dial :exhaust @20:08:37.76 Exhaust.select :open @20:08:37.87 SS.seek 0ml @20:08:47.19 Exhaust.select :close @20:08:47.30 SSV.dial CBV @20:08:47.34 CBV.dial SSV @20:08:48.10 Allowing 130s for 25.0ml of remaining 650.0ml @20:08:48.11 Intake.select :open @20:08:50.28 Intake @14.9psia, vacuum from 9.4 to 12.4psi @20:08:50.29 SS.reconfigure :inhaling 0.42ml/s @20:08:50.34 SS.seek 25ml @20:09:41.68 Vacuum too high -- waiting up to 40 seconds @20:09:41.70 SS.reconfigure :exhale @20:09:41.75 SS.hold @20:09:46.90 Intake @14.0psia, vacuum from 8.5 to 11.5psi @20:09:46.92 SS.reconfigure :inhaling 0.38ml/s @20:09:46.97 SS.seek 25ml @20:09:57.60 Stroke completed -- waiting for pressure to equalize ... @20:09:57.61 SS.reconfigure :exhale @20:09:57.66 SS.hold @20:10:10.39 Intake.select :close @20:10:11.03 SSV.dial :exhaust @20:10:11.80 Exhaust.select :open @20:10:11.92 SS.seek 0ml @20:10:21.43 Exhaust.select :close @20:10:21.54 SSV.dial CBV @20:10:21.57 CBV.dial SSV @20:10:22.40 Allowing 130s for 25.0ml of remaining 625.0ml @20:10:22.41 Intake.select :open @20:10:24.58 Intake @15.4psia, vacuum from 9.9 to 12.9psi @20:10:24.60 SS.reconfigure :inhaling 0.38ml/s @20:10:24.65 SS.seek 25ml @20:11:32.47 Stroke completed -- waiting for pressure to equalize ... @20:11:32.48 SS.reconfigure :exhale @20:11:32.53 SS.hold @20:11:50.87 Intake.select :close @20:11:51.03 SSV.dial :exhaust @20:11:51.81 Exhaust.select :open @20:11:51.93 SS.seek 0ml @20:12:00.94 Exhaust.select :close @20:12:01.05 SSV.dial CBV @20:12:01.08 CBV.dial SSV @20:12:01.89 Allowing 130s for 25.0ml of remaining 600.0ml @20:12:01.91 Intake.select :open @20:12:04.08 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:12:04.10 SS.reconfigure :inhaling 0.38ml/s @20:12:04.14 SS.seek 25ml @20:13:12.27 Vacuum too high -- waiting up to 40 seconds @20:13:12.29 SS.reconfigure :exhale @20:13:12.34 SS.hold @20:13:15.99 Intake @13.6psia, vacuum from 8.1 to 11.1psi @20:13:16.01 SS.reconfigure :inhaling 0.33ml/s @20:13:16.06 SS.seek 25ml @20:13:16.45 Stroke completed -- waiting for pressure to equalize ... @20:13:16.46 SS.reconfigure :exhale @20:13:16.51 SS.hold @20:13:33.37 Intake.select :close @20:13:33.53 SSV.dial :exhaust @20:13:34.30 Exhaust.select :open @20:13:34.41 SS.seek 0ml @20:13:43.81 Exhaust.select :close @20:13:43.92 SSV.dial CBV @20:13:43.95 CBV.dial SSV @20:13:44.75 Allowing 130s for 25.0ml of remaining 575.0ml @20:13:44.76 Intake.select :open @20:13:46.93 Intake @15.6psia, vacuum from 10.0 to 13.0psi @20:13:46.94 SS.reconfigure :inhaling 0.33ml/s @20:13:46.99 SS.seek 25ml @20:15:04.12 Stroke completed -- waiting for pressure to equalize ... @20:15:04.13 SS.reconfigure :exhale @20:15:04.18 SS.hold @20:15:31.02 Intake.select :close @20:15:31.18 SSV.dial :exhaust @20:15:31.96 Exhaust.select :open @20:15:32.07 SS.seek 0ml @20:15:41.06 Exhaust.select :close @20:15:41.17 SSV.dial CBV @20:15:41.21 CBV.dial SSV @20:15:41.97 Allowing 130s for 25.0ml of remaining 550.0ml @20:15:41.98 Intake.select :open @20:15:44.15 Intake @16.3psia, vacuum from 10.0 to 13.0psi @20:15:44.17 SS.reconfigure :inhaling 0.33ml/s @20:15:44.23 SS.seek 25ml @20:16:26.69 recoverPRVreagent08 @20:16:26.70 PTV.dial :mixing @20:16:26.83 PBV.dial :waste1 @20:16:27.22 delay 30 seconds @20:16:28.24 Vacuum too high -- waiting up to 40 seconds @20:16:28.26 SS.reconfigure :exhale @20:16:28.31 SS.hold @20:16:34.08 Intake @15.1psia, vacuum from 9.6 to 12.6psi @20:16:34.10 SS.reconfigure :inhaling 0.30ml/s @20:16:34.15 SS.seek 25ml @20:16:57.24 PBV.dial between PSV and waste1 @20:16:57.63 PSV.dial :air @20:16:58.10 PS.reconfigure :fast @20:16:58.15 PS.seek 2.5ml @20:17:11.38 Stroke completed -- waiting for pressure to equalize ... @20:17:11.40 SS.reconfigure :exhale @20:17:11.45 SS.hold @20:17:22.08 PSV.dial :mixloop @20:17:22.55 delay 2 seconds @20:17:24.56 PSV.dial :air @20:17:25.03 delay 2 seconds @20:17:27.05 PSV.dial :mixloop @20:17:27.52 delay 2 seconds @20:17:29.54 PSV.dial :air @20:17:30.03 PS.seek 0ml @20:17:51.64 SS.reconfigure :default @20:17:51.70 SS.hold @20:17:51.77 Intake.select :close @20:17:51.93 ShallowSampler::Clogged in WCR -- Intake @16psia & Outlet @14psia won't equalize after 475.0ml @20:17:51.95 SSV.dial :exhaust @20:17:52.71 Exhaust.select :open @20:17:52.83 SS.reconfigure :exhale @20:17:52.88 SS.seek 0ml @20:17:53.33 washCycleDA #1 of 3 @20:17:53.34 PSR.select :air @20:17:53.36 PSV.dial PSR @20:17:54.70 Pulling 0.5ml of PSR.wash @20:17:54.71 PSR.select :wash @20:17:54.84 PS.reconfigure :default @20:17:54.88 PS.seek 0.5ml @20:18:00.89 delay 5 seconds @20:18:01.90 Exhaust.select :close @20:18:02.02 Sampled 475.0ml @20:18:02.04 Drying puck before fixing for 4:00 CBV.dial :waste2b @20:18:02.81 delay 1:00 @20:18:05.91 PSR.select :air @20:18:06.03 PS.seek 2ml @20:18:22.20 PTV.dial :puck @20:18:22.95 PSV.dial PTV @20:18:23.70 PBV.dial :waste1 @20:18:24.15 PS.seek 1.5ml @20:18:30.05 delay 3 seconds @20:18:33.07 PBV.dial between PSV and waste1 @20:18:33.46 PS.seek 0ml @20:18:49.61 delay 2:00 @20:19:02.83 CSV.dial :air @20:19:03.29 CS.reconfigure :fast @20:19:03.34 CS.seek 10ml @20:19:27.30 Pumping wcr puck from tube 4 up to 35psi... @20:19:27.32 CBV.dial SSV @20:19:28.59 CBV.dial between CSV and waste2b @20:19:29.56 CSV.dial between air and CTV @20:19:29.89 CS.seek 6.49ml @20:19:38.81 CTV.dial CSV,avoiding: :air @20:19:38.83 CSV.dial CTV @20:19:40.11 CS.reconfigure :pressurize @20:19:40.19 CS.seek 0ml @20:19:55.70 CBV.dial :waste2b @20:19:56.03 CS.reconfigure :default @20:19:56.08 CS.seek 0ml @20:19:58.36 delay 1:00 @20:20:49.63 recoverPSRreagent08 @20:20:49.64 PTV.dial :puck @20:20:49.72 PBV.dial :waste1 @20:20:50.11 delay 30 seconds @20:20:58.39 CTV.dial :air,avoiding: :intake @20:20:59.78 Fix #1 of 1 (with RNAlater) for 4:00 @20:20:59.79 CSV.dial CSR @20:21:00.35 Pulling 2ml of CSR.RNAlater @20:21:00.36 CSR.select :RNAlater @20:21:00.48 CS.seek 2ml @20:21:06.36 delay 5 seconds @20:21:11.38 CSR.select :air @20:21:11.51 CS.reconfigure :fast @20:21:11.56 CS.seek 10ml @20:21:20.13 PBV.dial between PSV and waste1 @20:21:20.53 PSR.select :air @20:21:20.55 PSV.dial PSR @20:21:21.30 PS.reconfigure :fast @20:21:21.35 PS.seek 2.5ml @20:21:30.84 Pumping wcr puck from tube 4 up to 35psi... @20:21:30.86 CBV.dial SSV @20:21:32.10 CBV.dial between CSV and waste2b @20:21:33.08 CSV.dial between air and CTV @20:21:33.67 CS.seek 6.303ml @20:21:43.18 CTV.dial CSV,avoiding: :air @20:21:43.21 CSV.dial CTV @20:21:44.62 CS.reconfigure :pressurize @20:21:44.67 CS.seek 0ml @20:21:45.11 PSV.dial PTV @20:21:45.86 delay 5 seconds @20:21:50.88 PSV.dial PSR @20:21:51.62 delay 5 seconds @20:21:56.64 PSV.dial PTV @20:21:57.40 delay 5 seconds @20:21:57.73 delay 4:00 @20:22:02.42 PSV.dial :air @20:22:03.21 PS.seek 0ml @20:22:26.36 washCycleDA #2 of 3 @20:22:26.37 PSR.select :air @20:22:26.39 PSV.dial PSR @20:22:27.72 Pulling 0.5ml of PSR.wash @20:22:27.73 PSR.select :wash @20:22:27.84 PS.reconfigure :default @20:22:27.89 PS.seek 0.5ml @20:22:34.33 delay 5 seconds @20:22:39.35 PSR.select :air @20:22:39.47 PS.seek 2ml @20:22:55.67 PTV.dial :puck @20:22:55.68 PSV.dial PTV @20:22:56.42 PBV.dial :waste1 @20:22:56.87 PS.seek 1.5ml @20:23:02.79 delay 3 seconds @20:23:05.81 PBV.dial between PSV and waste1 @20:23:06.21 PS.seek 0ml @20:23:22.39 delay 2:00 @20:25:22.42 recoverPSRreagent08 @20:25:22.43 PTV.dial :puck @20:25:22.44 PBV.dial :waste1 @20:25:22.82 delay 30 seconds @20:25:52.85 PBV.dial between PSV and waste1 @20:25:53.24 PSR.select :air @20:25:53.26 PSV.dial PSR @20:25:54.02 PS.reconfigure :fast @20:25:54.07 PS.seek 2.5ml @20:25:57.75 CBV.dial :waste2b @20:25:58.10 CS.reconfigure :default @20:25:58.15 CS.seek 0ml @20:26:02.48 delay 1:00 @20:26:17.82 PSV.dial PTV @20:26:18.57 delay 5 seconds @20:26:23.59 PSV.dial PSR @20:26:24.34 delay 5 seconds @20:26:29.36 PSV.dial PTV @20:26:30.11 delay 5 seconds @20:26:35.13 PSV.dial :air @20:26:35.89 PS.seek 0ml @20:26:59.17 washCycleDA #3 of 3 @20:26:59.18 PSR.select :air @20:26:59.19 PSV.dial PSR @20:27:00.52 Pulling 0.5ml of PSR.wash @20:27:00.54 PSR.select :wash @20:27:00.65 PS.reconfigure :default @20:27:00.70 PS.seek 0.5ml @20:27:02.51 CTV.dial :air,avoiding: :intake @20:27:06.66 delay 5 seconds @20:27:11.68 PSR.select :air @20:27:11.81 PS.seek 2ml @20:27:28.01 PTV.dial :puck @20:27:28.02 PSV.dial PTV @20:27:28.77 PBV.dial :waste1 @20:27:29.23 PS.seek 1.5ml @20:27:35.10 delay 3 seconds @20:27:38.12 PBV.dial between PSV and waste1 @20:27:38.52 PS.seek 0ml @20:27:54.63 delay 2:00 @20:29:54.66 recoverPSRreagent08 @20:29:54.67 PTV.dial :puck @20:29:54.68 PBV.dial :waste1 @20:29:55.05 delay 30 seconds @20:30:25.08 PBV.dial between PSV and waste1 @20:30:25.46 PSR.select :air @20:30:25.48 PSV.dial PSR @20:30:26.23 PS.reconfigure :fast @20:30:26.28 PS.seek 2.5ml @20:30:49.79 PSV.dial PTV @20:30:51.02 delay 5 seconds @20:30:56.04 PSV.dial PSR @20:30:56.79 delay 5 seconds @20:31:01.81 PSV.dial PTV @20:31:02.59 delay 5 seconds @20:31:07.60 PSV.dial :air @20:31:08.37 PS.seek 0ml @20:31:31.48 Gate.power :camera,:ON @20:31:31.49 Mixing DA Substrates @20:31:31.50 PSR.select :air @20:31:31.52 PSV.dial PSR @20:31:32.92 Pulling 0.25ml of PSR.sub1 @20:31:32.94 PSR.select :sub1 @20:31:33.13 PS.reconfigure :default @20:31:33.26 PS.seek 0.25ml @20:31:36.70 delay 5 seconds @20:31:41.72 Pulling 0.25ml of PSR.sub2 @20:31:41.74 PSR.select :sub2 @20:31:41.86 PS.seek 0.5ml @20:31:45.26 delay 5 seconds @20:31:50.28 PSR.select :air @20:31:50.39 PS.seek 2ml @20:32:06.47 PTV.dial :puck @20:32:06.49 PSV.dial PTV @20:32:07.24 PBV.dial :waste1 @20:32:07.69 PS.seek 1.5ml @20:32:13.66 delay 3 seconds @20:32:16.68 PBV.dial between PSV and waste1 @20:32:17.08 PS.seek 0ml @20:32:33.19 delay 10 seconds @20:32:43.22 recoverPSRreagent08 @20:32:43.23 PTV.dial :puck @20:32:43.24 PBV.dial :waste1 @20:32:43.63 delay 30 seconds @20:33:13.66 PBV.dial between PSV and waste1 @20:33:14.05 PSR.select :air @20:33:14.07 PSV.dial PSR @20:33:14.95 PS.reconfigure :fast @20:33:15.00 PS.seek 2.5ml @20:33:38.76 PSV.dial PTV @20:33:39.51 delay 5 seconds @20:33:44.53 PSV.dial PSR @20:33:45.29 delay 5 seconds @20:33:50.31 PSV.dial PTV @20:33:51.07 delay 5 seconds @20:33:56.09 PSV.dial :air @20:33:56.86 PS.seek 0ml @20:34:20.44 Additional air clear @20:34:20.45 PSR.select :air @20:34:20.47 PSV.dial PSR @20:34:21.82 Pulling 2ml of PSR.air @20:34:21.83 PSR.select :air @20:34:21.85 PS.reconfigure :default @20:34:21.90 PS.seek 2ml @20:34:43.13 delay 5 seconds @20:34:48.15 PTV.dial :puck @20:34:48.17 PSV.dial PTV @20:34:48.91 PBV.dial :waste1 @20:34:49.36 PS.seek 1.5ml @20:34:55.29 delay 3 seconds @20:34:58.30 PBV.dial between PSV and waste1 @20:34:58.69 delay 1 second @20:34:59.71 PSV.dial :waste1 @20:35:00.76 PS.seek 0ml @20:35:16.96 Processing Puck Heater at 28C before imaging @20:35:16.97 PH.coast @20:35:17.08 Imaging da puck in Processing Clamp while storing it to tube 3 @20:35:17.76 SC.reconfigure :tight @20:35:17.81 SC.seek 3 @20:35:21.45 SC.configure :limp @20:35:21.68 Elbow.reconfigure :tight @20:35:21.73 Elbow.seek 3 @20:35:23.44 Forearm.reconfigure :tight @20:35:23.49 Forearm.seek 3 + 800 counts @20:35:24.50 SE.reconfigure :pick @20:35:24.56 SE.seek :beamBreak @20:35:26.04 SE.configure :default @20:35:26.20 Elbow.reconfigure :limp @20:35:26.25 Forearm.reconfigure :loose @20:35:26.30 Forearm.seek 3 @20:35:26.71 Elbow.hold @20:35:26.77 Elbow.reconfigure :tight @20:35:26.87 SE.seek 17.11 Pucks below Top Plate @20:35:27.46 Forearm.force -20 @20:35:27.51 Forearm.seek 3 + 290 counts @20:35:27.79 Forearm.reconfigure :tight @20:35:27.84 Forearm.seek 3 + 800 counts @20:35:28.10 SE.seek 18.11 Pucks below Top Plate @20:35:28.79 Forearm.reconfigure :loose @20:35:28.84 Forearm.seek :retract @20:35:29.76 Elbow.seek PC @20:35:32.31 Elbow.reconfigure :loose @20:35:32.41 Forearm.seek PC @20:35:33.52 Hand.close @20:35:34.24 PC.seek :open @20:35:38.80 Forearm.seek :retract @20:35:39.82 Elbow.reconfigure :tight @20:35:40.30 Elbow.seek 3 @20:35:43.01 Forearm.reconfigure :tight @20:35:43.06 Forearm.seek 3 + 800 counts @20:35:44.06 Hand.open @20:35:44.93 SE.seek 17.87 Pucks below Top Plate @20:35:45.19 SE.reconfigure :seal @20:35:45.24 SE.seek 17.21 Pucks below Top Plate @20:35:45.79 Lights, camera, ACTION! @20:35:45.82 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da25dec1020h600ml.tif @20:36:05.30 Waiting >20 seconds for da to relinquish FlushPuck @20:36:13.56 SX HX9/16 (USB:15): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 24.406 seconds /var/log/esp/da25dec1020h600ml.tif: TIFF Upload Complete @20:36:13.60 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da25dec1020h600ml15s.tif @20:36:29.25 SX HX9/16 (USB:15): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da25dec1020h600ml15s.tif: TIFF Upload Complete @20:36:29.27 PuckCamera: snap -bin=2,2 21.0 /var/log/esp/midres/da25dec1020h600ml21s.tif @20:36:50.92 SX HX9/16 (USB:15): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21 seconds /var/log/esp/midres/da25dec1020h600ml21s.tif: TIFF Upload Complete @20:36:50.94 Gate.power :camera,:OFF @20:36:50.97 DAfans.email "24.406 sec auto-exposure",Subject: "da25dec1020h600ml images!" @20:36:51.40 Elbow.seek 3 @20:36:51.90 Forearm.seek 3 + 800 counts @20:36:52.13 SE.reconfigure :gently @20:36:52.20 SE.seek 17.92 Pucks below Top Plate @20:36:53.10 Elbow.reconfigure :limp @20:36:53.15 Forearm.reconfigure :loose @20:36:53.21 Forearm.seek 3 @20:36:53.64 Elbow.hold @20:36:53.73 Elbow.reconfigure :tight @20:36:53.86 SE.reconfigure :default @20:36:53.96 SE.seek 18.11 Pucks below Top Plate @20:36:54.38 Forearm.force -20 @20:36:54.43 Forearm.seek 3 + 280 counts @20:36:54.84 Forearm.reconfigure :tight @20:36:54.91 Forearm.seek 3 + 800 counts @20:36:55.36 SE.seek :down @20:36:56.38 SE.stop @20:36:57.73 Forearm.reconfigure :loose @20:36:57.94 Forearm.seek :retract @20:36:59.11 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @20:36:59.78 Elbow.seek FlushPuck::Garage @20:37:01.67 Elbow.reconfigure :loose @20:37:01.87 Forearm.seek :garage @20:37:02.97 Hand.close @20:37:04.05 Forearm.seek :retract @20:37:05.11 Elbow.reconfigure :tight @20:37:05.21 Elbow.seek PC @20:37:06.58 Elbow.reconfigure :loose @20:37:06.79 Forearm.reconfigure :tight @20:37:06.89 Forearm.seek PC @20:37:08.57 PC.seek :closed @20:37:13.11 Processing Clamp onSpring/offSpring for 12/26 samples, on/off torque 1.54 @20:37:13.29 Hand.open @20:37:14.25 Forearm.reconfigure :loose @20:37:14.35 Forearm.seek :retract @20:37:15.50 PSR.select :air @20:37:15.56 Storing wcr puck in Collection Clamp to tube 3 @20:37:15.60 PSV.dial PSR @20:37:16.10 SC.reconfigure :tight @20:37:16.24 SC.seek 3 @20:37:16.29 Pulling 0.5ml of PSR.flush @20:37:16.35 PSR.select :flush @20:37:16.57 SC.configure :limp @20:37:17.91 PS.seek 0.5ml @20:37:18.19 Elbow.reconfigure :tight @20:37:18.29 Elbow.seek 3 @20:37:21.12 Forearm.reconfigure :tight @20:37:21.24 Forearm.seek 3 + 800 counts @20:37:22.52 SE.reconfigure :pick @20:37:22.64 SE.seek :beamBreak @20:37:23.94 delay 5 seconds @20:37:24.12 SE.configure :default @20:37:24.46 Elbow.reconfigure :limp @20:37:24.56 Forearm.reconfigure :loose @20:37:24.66 Forearm.seek 3 @20:37:25.10 Elbow.hold @20:37:25.23 Elbow.reconfigure :tight @20:37:25.44 SE.seek 18.1 Pucks below Top Plate @20:37:26.09 Forearm.force -20 @20:37:26.18 Forearm.seek 3 + 293 counts @20:37:26.77 Forearm.reconfigure :tight @20:37:26.87 Forearm.seek 3 + 800 counts @20:37:27.25 SE.seek 19.1 Pucks below Top Plate @20:37:27.83 upsync @20:37:27.93 Forearm.reconfigure :loose @20:37:27.98 Forearm.seek :retract @20:37:28.88 Elbow.seek CC @20:37:28.97 PSR.select :air @20:37:29.09 PS.seek 2.5ml @20:37:30.44 Elbow.reconfigure :loose @20:37:30.54 Forearm.seek CC @20:37:31.56 Hand.close @20:37:32.30 CC.seek :open @20:37:36.53 Forearm.seek :retract @20:37:37.60 Elbow.reconfigure :tight @20:37:37.65 Elbow.seek 3 @20:37:39.38 Elbow.reconfigure :limp @20:37:39.44 Forearm.seek 3 @20:37:40.55 Elbow.hold @20:37:40.67 Elbow.reconfigure :tight @20:37:40.72 Hand.open @20:37:41.62 Forearm.force -20 @20:37:41.66 Forearm.seek 3 + 320 counts @20:37:41.95 Forearm.reconfigure :tight @20:37:42.00 Forearm.seek 3 + 800 counts @20:37:42.33 SE.seek :down @20:37:43.22 SE.stop @20:37:43.47 Forearm.reconfigure :loose @20:37:43.52 Forearm.seek :retract @20:37:44.44 Starting finalPurge @20:37:50.56 delay 1 second @20:37:51.58 PRV.dial :waste2,avoiding: :ab2 @20:37:53.45 PTV.dial :PRVmixing @20:37:54.77 PSV.dial :mixloop @20:37:55.80 PS.seek :empty @20:38:03.58
Waiting >20 seconds for psFlush to relinquish FlushPuck @20:38:22.37 PSR.select :air @20:38:22.39 PSV.dial PSR @20:38:23.44 Pulling 0.5ml of PSR.flush @20:38:23.45 PSR.select :flush @20:38:23.57 PS.seek 0.5ml @20:38:29.47 delay 5 seconds @20:38:34.49 PSR.select :air @20:38:34.61 PS.seek 2.5ml @20:38:55.91 delay 1 second @20:38:56.93 PBV.dial :waste1 @20:38:57.35 PTV.dial :mixing @20:38:58.10 PSV.dial :mixloop @20:38:59.15 PS.seek :empty @20:39:25.56 PSV.dial PSR @20:39:26.61 PSR.select :flush @20:39:26.74 PS.seek 1ml @20:39:37.78 PSR.select :air @20:39:37.90 PS.seek 2.5ml @20:39:54.09 PSV.dial PTV @20:39:54.84 PTV.dial :puck @20:39:55.58 PBV.dial :waste1 @20:39:55.66 PS.seek 0ml @20:40:22.03 PSV.dial PSR @20:40:22.78 PSR.select :flush @20:40:22.90 PS.seek 0.5ml @20:40:28.78 PSR.select :air @20:40:28.90 PS.seek 2.5ml @20:40:50.22 PSV.dial PTV @20:40:50.97 PBV.dial PSV @20:40:51.55 PS.seek 0ml @20:41:17.94 PSV.dial PBV @20:41:18.52 PS.seek 2.5ml @20:41:44.91 PSV.dial :waste1 @20:41:45.66 PS.seek 0ml @20:42:12.03 PSV.dial PSR @20:42:12.48 PSR.select :air @20:42:12.51 PS.seek 2.5ml @20:42:38.87 PSV.dial PTV @20:42:39.61 PBV.dial PSV @20:42:39.69 PS.seek 0ml @20:43:06.00 PSV.dial PBV @20:43:06.58 PS.seek 2.5ml @20:43:32.90 PSV.dial :waste1 @20:43:33.66 PS.seek 0ml @20:43:59.97 PSV.dial PSR @20:44:00.42 PSR.select :flush @20:44:00.54 PS.seek 1ml @20:44:11.56 PSR.select :air @20:44:11.67 PS.seek 2.5ml @20:44:27.81 PSV.dial PTV @20:44:28.54 PTV.dial :puck @20:44:28.62 PBV.dial :waste1 @20:44:29.27 PS.seek 0ml @20:44:55.62 PSV.dial PSR @20:44:56.37 PSR.select :flush @20:44:56.49 PS.seek 0.5ml @20:45:02.47 PSR.select :air @20:45:02.58 PS.seek 2.5ml @20:45:23.87 PSV.dial PTV @20:45:24.64 PBV.dial PSV @20:45:25.21 PS.seek 0ml @20:45:51.56 PSV.dial PBV @20:45:52.03 PS.seek 2.5ml @20:46:18.37 PSV.dial :waste1 @20:46:19.11 PS.seek 0ml @20:46:45.40 PSV.dial PSR @20:46:45.87 PSR.select :air @20:46:45.90 PS.seek 2.5ml @20:47:12.31 PSV.dial PTV @20:47:13.04 PBV.dial PSV @20:47:13.13 PS.seek 0ml @20:47:39.49 PSV.dial PBV @20:47:39.95 PS.seek 2.5ml @20:48:06.31 PSV.dial :waste1 @20:48:07.05 PS.seek 0ml @20:48:33.50
Loading FlushPuck into Collection Clamp from Processing Clamp @20:48:33.69 Elbow.seek PC @20:48:36.19 Elbow.reconfigure :loose @20:48:36.29 Forearm.seek PC @20:48:37.39 Hand.close @20:48:38.10 PC.seek :open @20:48:42.58 Forearm.seek :retract @20:48:43.60 Elbow.reconfigure :tight @20:48:43.65 Elbow.seek CC @20:48:47.26 Elbow.reconfigure :loose @20:48:47.36 Forearm.reconfigure :tight @20:48:47.41 Forearm.seek CC @20:48:49.11 CC.seek :closed @20:48:53.11 Collection Clamp onSpring/offSpring for 6/27 samples, on/off torque 2.04 @20:48:53.18 Hand.open @20:48:54.04 Forearm.reconfigure :loose @20:48:54.09 Forearm.seek :retract @20:48:55.07 Flushing the Collection Valve::Manifold @20:48:55.20 CSV.dial CSR @20:48:55.64 Pulling 5ml of CSR.flush @20:48:55.65 CSR.select :flush @20:48:55.76 CS.seek 5ml @20:49:09.43 delay 5 seconds @20:49:14.45 CSR.select :air @20:49:14.62 CS.reconfigure :fast @20:49:14.67 CS.seek 7.5ml @20:49:21.21 CSV.dial :waste @20:49:22.50 CS.reconfigure :default @20:49:22.55 CS.seek 0ml @20:49:42.50 Flushing lower manifold's waste1 port @20:49:42.62 CSV.dial :flush @20:49:43.06 CS.reconfigure :fast @20:49:43.11 CS.seek 5ml @20:49:55.37 delay 5 seconds @20:50:00.39 CSV.dial :air @20:50:00.91 CS.seek 7ml @20:50:06.40 CSV.dial CTV @20:50:06.43
CTV.dial CSV @20:50:07.85 CBV.dial :waste1 @20:50:08.64 CS.seek 0ml @20:50:25.47 Flushing lower manifold's waste2 port @20:50:25.59 CSV.dial :flush @20:50:26.31 CS.seek 5ml @20:50:38.65 delay 5 seconds @20:50:43.67 CSV.dial :air @20:50:44.18 CS.seek 7.001ml @20:50:49.59 CSV.dial CTV @20:50:49.62
CTV.dial CSV @20:50:50.07 CBV.dial :waste2 @20:50:51.13 CS.seek 0ml @20:51:08.31 Flushing lower manifold's waste2b port @20:51:08.43 CSV.dial :flush @20:51:09.17 CS.seek 5ml @20:51:21.49 delay 5 seconds @20:51:26.51 CSV.dial :air @20:51:27.04 CS.seek 7ml @20:51:32.50 CSV.dial CTV @20:51:32.52
CTV.dial CSV @20:51:32.98 CBV.dial :waste2b @20:51:34.03 CS.seek 0ml @20:51:50.78 Disinfecting external Intake with kill @20:51:50.90 CSV.dial CSR @20:51:51.34 Pulling 8ml of CSR.kill @20:51:51.35 CSR.select :kill @20:51:51.46 CS.seek 8ml @20:52:10.56 delay 5 seconds @20:52:15.58 CSR.select :air @20:52:15.70 CS.seek 10ml @20:52:21.15 CSV.dial CBV @20:52:21.19
CBV.dial CSV @20:52:22.23 CTV.dial :intake @20:52:23.01 Intake.select :open @20:52:23.12 CS.seek 2ml @20:52:42.12 Intake.select :close @20:52:42.22 CTV.dial :air @20:52:43.00 CS.reconfigure :default @20:52:43.05 CS.seek 0ml @20:52:49.09 CSV.dial CSR @20:52:50.10 Pulling 5ml of CSR.flush @20:52:50.11 CSR.select :flush @20:52:50.23 CS.seek 5ml @20:53:03.90 delay 5 seconds @20:53:08.92 CSR.select :air @20:53:09.04 CS.seek 10ml @20:53:22.65 CSV.dial CBV @20:53:23.67 CS.seek 0ml @20:53:50.15 Garaging FlushPuck in Collection Clamp @20:53:50.22 Elbow.reconfigure :tight @20:53:50.27 Elbow.seek CC @20:53:50.45 Elbow.reconfigure :loose @20:53:50.55 Forearm.seek CC @20:53:51.56 Hand.close @20:53:52.35 CC.seek :open @20:53:56.46 Forearm.seek :retract @20:53:57.46 Elbow.reconfigure :tight @20:53:57.95 Elbow.seek :garage @20:54:00.79 Elbow.reconfigure :loose @20:54:00.90 Forearm.reconfigure :tight @20:54:00.94 Forearm.seek :garage @20:54:02.02 Hand.open @20:54:02.78 Forearm.reconfigure :loose @20:54:02.83 Forearm.seek :retract @20:54:03.92 Completed [initialPurge; da [1000, 1000] #da and wsr are run simultanelously] begun 18:34:11 @20:54:03.98 Asleep after completing DA_WCR_121025 assays @20:54:03.99 GoodNews.email "{1 => 22,\n 2 => 22,\n 3 => 20,\n 4 => 2,\n 5 => 22,\n 6 => 22,\n 7 => 22}\nCTD data unavailable\nCan@20:53:32, 41% flow, 18.9C, 30% humidity, 14.5psia, 13.71V, 0.378A avg, 432.209Ah, 5.18W\nSampled 475.0ml",Subject: "Asleep after completing DA_WCR_121025 assays"